CNTLN cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	CNTLN
Entrez Gene ID	102403185
Full Name	centlein
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006067955.1	XP_006068017.1	centlein
XM_025281478.1	XP_025137263.1	centlein isoform X2
XM_025281480.1	XP_025137265.1	centlein isoform X4
XM_025281477.1	XP_025137262.1	centlein isoform X1
XM_025281479.1	XP_025137264.1	centlein isoform X3

Pathways from BioSystems
Gene Ontology

The following CNTLN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNTLN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu23036	XM_006067955.1 Latest version!	Bubalus bubalis centlein, centrosomal protein (CNTLN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu62687	XM_025281477.1 Latest version!	Bubalus bubalis centlein (CNTLN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu62688	XM_025281478.1 Latest version!	Bubalus bubalis centlein (CNTLN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu62689	XM_025281479.1 Latest version!	Bubalus bubalis centlein (CNTLN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu62690	XM_025281480.1 Latest version!	Bubalus bubalis centlein (CNTLN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CNTLN ( XM_006067955.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006068017.1 Bubalus bubalis centlein, centrosomal protein (CNTLN), mRNA.

Price & Availability↑

CloneID	OBu23036
Clone ID Related Accession (Same CDS sequence)	XM_006067955.1
Accession Version	XM_006067955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	centlein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785284.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC GGTCGCCGCC CTCACAGCTC CCTCCGCCCC CGGCGCGACA CTTGGGACCC 
TGGTCCCCAC GTGTTGGTCG GGGAGCTGTG GCACACGCAG TGCGCAGCGA GACCTCGGGT 
CTTGAGGGCG CAGCGCGGGA GGGCGTCGTG GACAAAAGCG ATTTACCCTG GCGGGGCGAA 
GAAGGGTCAG AGGGCCAGCG AGGGCCAGCG GGGACAGCTC CGGCTCAGGC TCCCCTCCTC 
AGCGCGCCCA TGGGGTCCAT GCGGCTAGAG GGCATCTCGG TAGAGGAGGC GATGGTGACC 
CGGACGCAGC TGCTGGAGGA AGAGCTGAGC AGCGTAAAGG AGGAGCTGGC CCTGTGTCAG 
GCTGATAAAG AATTCGTATG GTCTTTGTGG AAACGCCTCC AGGTGACAAA TCCAGATGTC 
ACACAAGCAG TCAGTTTGGT TGTGGAAAGA GAAAAACGGA AATCTGAAGC TAAAGACAGA 
AAAGTTTTAG AAATTCTGCA AGTCAAGGAT GCTAAAATAC AAGAATTGGA ACAGAGAGAA 
TCAGGACTAA CACAGGAAAT AAATGACCTT GTAAAACGGA AGATTGCAGT AGATGAAGAA 
AATGATTTCT TAAGGAAAGA ATTCGGTGAC TTGCAGAAGA AATTTAAAGA GAAAAGTCAA 
GAAGTTAAGG ACACTAAGGA GTGTGTGCAG ATTAAGGAAG AGCAAAACAG GCTGGTTATA 
AAAAATCTGA AGGAGGAAAA TGAGAAATTA AGTATCCACT GCGCTGACCT GCTAAATGAG 
CTGGAGAAAC TGAGGAAGCA GGAAGCACAT TGGAGAAAAG AAAAATTTAG CATTGATGCA 
AAAGTAAAGG CCTTTGAAGA CAATTTAATT GAAGCAAGGA AAGAAATTGA AGTATCACAG 
AGCAAATATA ATGCACTATC ATTACAGCTG AATAATAAAC AGACAGAACT TATGCAGAAG 
GATATGGATA TTACCTTGGT CAGGCAAGAG TTGCAGGAGC TACAGAATCT TTACAAACAG 
AACAGTGCAC ATACAGCACA GCAAGCCGAG CTGATCCAGC AGCTTCAGGT TCTGAATATG 
GACACACAGA AAGTACTGAG GAACCAGGAA GATGTTCACA CAGCTGAAAG TATATCGTAT 
CAAAAACTTT ACAATGAATT ACATATTTGT TTTGAAACCA CAAAATCAAG TGAAGCTATG 
CTCCGACAAA GTGTTGTTAC TCTTCAGGAT CAGCTATTTC AAAAAGAGCA AGAAAATGCT 
AAATTAAAAG AAAAGCTTCA GCAATCACAA GAAACACTCT ATCCTTTACC TCGAGAATGT 
GATTCAGACC GCTCAGAACA GGTTTCTCAA CAGCCATCTT TATCAAGCTT AGAAACCTTA 
ATGGTCTCAC AGAAGTCTGA AATTGAGTAT TTACAGGAGA AACTGAAAAT AGCAAATGAA 
AAACTGTCAG AGAACAGATC TACCAACAAG GGTTCCTCAG AGAAGAGCAT CTTGACAAGT 
ACTGAAGGGA AACGTAAGGA ACCACCTGTA AAACGTTCAA GGTCTTTGTC CCCAAAGAGC 
TCTTTCGCAG ACTCGGAGGA ACTTAAGAAG CTGAAGAAAG CTGAAAGAAG GATTGAAAAC 
TTAGAGAAGG CACTACAACT AAAGAACCAA GAAAATGATG AGCTAAGAGA TGCCCATGAA 
AAACGCAAAG AAAGGCTACA GATGTTACAG ACCAACTACA GAGCAGTAAA AGAGCAATTA 
AAACAGTGGG AAGAAGGCAG TGGCATGATG GAAATCAGGA AAGTAAAGAG AGCAGATCCT 
CAACAACTTC AACAAGAAGA TTCTGATGCT GTATGGAATG AACTGGCCTA TTTCAAAAGG 
GAGAACCAGG AGCTAATGAT TCAAAAAATG CATCTTCAAG AAGAACTGGA TAAACTTAAA 
GTACATATGT CTGTTGATAA AGCAACAATA CAAGAATTGA ATACATTTAT GGCAGAGAGA 
AGAGAAGAAC AACTCTTTAG ACACGATGAA GATGATGGGG TCAAGAAAAG TACTCCAGAG 
AAGAATTGGA AAGAAATGTC GAAGCAGACA TTACAGAAGG TCATTGAATT GGAAAATCGG 
CTAAAATCTT TTGAGAAAAG GTCAAGAAAA TTAAAAGAAG GGAATAAAAA TTTAATAAAA 
GAAAATGATT TCCTGAAATC TCTTGTTAAA GAGCAGCAAG AAGATGCAAA GACCAGAGAA 
AAAGAGCTGG AACAATTAAA AAAGGGAAGT AAAGGTGTGG AAAAAGACAA AGCTAAACTT 
GAGGCAAAAA TCAGTGAGCT GGAGATAGAA GTCATTTCCC TAAGGAGACA AGTGGCAGAA 
GCTAATGAGT TGAGAAATGA AAATGAAGAG CTGATGAATT CAATGGAGAA ATTACAGCAT 
TCAGCGGACA AAGCTAAATC TGAGATGGCC ACCACGGAAG CGCGATCTGG ATCTGGGCAA 
TCTGAGTGTA AGACAACCAC TACCAAGGTT AAATTTAAAA CAGCCAAGAA AAAGTGCTCT 
GTGGGTCGTT ACCACACTGT TCTCAATCAC TCCATCAAGG TCATGAGCAA TGTGTTTGAG 
AACCTCAACA AGGACGACTG GGAGGACGTG AGTGAAAGCA GTGATTCTGA AACACAGAAC 
TTACGAAATT TGGGAACAAT TATTGTAGAA ACATCCCAAA AAATAAGTCC AACAGAAGAC 
AGACAAGACC AGAAAGAACG TGACCAAACG GAGGACAGCC AAATGCAAGG AAAAGAAATA 
GTACAGATAT ATCCAAATGT AGAGGACAAG ACCCCAAAGA ATTATTTTCA CAAGAATGCC 
AAAAAATCAG CTCTTCAAAA GAAGAATAGC AGTATTCAAA AGACTTCACA TACAGCAGGT 
CCTACCAGAG TTAACAGAGA AAAGTGGAAA AATATAACTG CCCAGAAATC AAATAGTAAT 
GTTATTTTAT TACGAGAACG GATTGTATCC TTGCAACAGC AAAACAGTGT GCTTTTGAAT 
GCCAAAAAAG CAGCAGAACT GGCTGTTAAA GAATATAAAG AAGTCAACGA CAAGCTCCTG 
CATCAGCAGC AAGTGTCTGA TCAACGATTT CAAACAAGCA GACAGACAAT AAAGAAGCTA 
AATTCGGATT TGGCTGAGCT TCAAAAAGAA AAAGAAGATT TACTAAAGAG AGTAGAATCT 
TCATCTGAAA TCACATGTTT GACTGAAGAA GTTGCCCAGG TAACAGTTCC ACGGATACAG 
ATTACATCAC TTGGCCCTTC AAGGAGCATG GATTTGGAAA TGAAGCAATT GCAGTGTAAA 
CTAAAGAATG CAACTAATGA ACTCACTAAA CAATCTTCAA ACTTGAAGTC TCTGAAATTT 
GAACTCCTAG CAAAAGAAGA ACACATAAAG GAAATGCATG AAAAGATGTC TCGAATGGAG 
AGAGATATAA CCATGAAAAG ACATTTGATA GAGGACTTGA AATTTCGACA GAAAGTAAAT 
TTGGAAAGTA ATGAGAGTAT TAATGAAATG CTAGAAAATC TTGAAAAAAA GGTGAAGACA 
TTAACTGAGG AGTGTTCCAA CAAGAAAGTA TCAATTGATT CACTAAAGCA AAGACTTAGC 
GTTGCTGTAA AAGAGAAGTC TCAGTATGAA GAGATGTATC AGCAAACTAA AGAGGAGTTA 
GAAAAAAAGG ATGTCAAGCT TACTTTCCTC CTGTCAAAAA TAAATGAGAC TGAATCTGCC 
ATGGCAGAGA TTGAAACAGC AGCATCTAAG CATCTTCAAG GATTAGCAAT GCAAAGTGAG 
CAGGCCCTAG AAGGTGCACA GAAGAAACTG CTGCTAGCCA GTGAGAAAGT AGAAGAGTTC 
ACCATATTTG TGAAGGCCTT GGTCAGAGAG TTACAAAATG ACATCCATTT GATTAGGCGA 
CAAATCAGAG AGCTTAAAAA GATGCAGAAA AACAGGGATG CTTGTAAAAC CTCAACCCAT 
AAAGCCCAGA CCTTAGCAGC TTCTATCCTG AACATTTCAC GATCGGACCT AGAGGAAATA 
CTGGACACAG AAGATGAAGC GGAAATTGAA AGGGCAAAGA CTGAGGCCGA AAATGACAAA 
GAATGGCTGT TGTACATTCG GAAACTTCTT GAAGGACAGC TTCCCTTTGC CACATATTTA 
CTAGAAGCAA TAATGGAAAA AATAAATGAA AAAAAGAGAC TAGTTGAAGA ATATTTTACA 
GTTATGAAAG ATACTAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006068017.1
CDS	49..4269
Translation	MAARSPPSQLPPPPARHLGPWSPRVGRGAVAHAVRSETSGLEGAAREGVVDKSDLPWRGEEGSEGQRGPAGTAPAQAPLLSAPMGSMRLEGISVEEAMVTRTQLLEEELSSVKEELALCQADKEFVWSLWKRLQVTNPDVTQAVSLVVEREKRKSEAKDRKVLEILQVKDAKIQELEQRESGLTQEINDLVKRKIAVDEENDFLRKEFGDLQKKFKEKSQEVKDTKECVQIKEEQNRLVIKNLKEENEKLSIHCADLLNELEKLRKQEAHWRKEKFSIDAKVKAFEDNLIEARKEIEVSQSKYNALSLQLNNKQTELMQKDMDITLVRQELQELQNLYKQNSAHTAQQAELIQQLQVLNMDTQKVLRNQEDVHTAESISYQKLYNELHICFETTKSSEAMLRQSVVTLQDQLFQKEQENAKLKEKLQQSQETLYPLPRECDSDRSEQVSQQPSLSSLETLMVSQKSEIEYLQEKLKIANEKLSENRSTNKGSSEKSILTSTEGKRKEPPVKRSRSLSPKSSFADSEELKKLKKAERRIENLEKALQLKNQENDELRDAHEKRKERLQMLQTNYRAVKEQLKQWEEGSGMMEIRKVKRADPQQLQQEDSDAVWNELAYFKRENQELMIQKMHLQEELDKLKVHMSVDKATIQELNTFMAERREEQLFRHDEDDGVKKSTPEKNWKEMSKQTLQKVIELENRLKSFEKRSRKLKEGNKNLIKENDFLKSLVKEQQEDAKTREKELEQLKKGSKGVEKDKAKLEAKISELEIEVISLRRQVAEANELRNENEELMNSMEKLQHSADKAKSEMATTEARSGSGQSECKTTTTKVKFKTAKKKCSVGRYHTVLNHSIKVMSNVFENLNKDDWEDVSESSDSETQNLRNLGTIIVETSQKISPTEDRQDQKERDQTEDSQMQGKEIVQIYPNVEDKTPKNYFHKNAKKSALQKKNSSIQKTSHTAGPTRVNREKWKNITAQKSNSNVILLRERIVSLQQQNSVLLNAKKAAELAVKEYKEVNDKLLHQQQVSDQRFQTSRQTIKKLNSDLAELQKEKEDLLKRVESSSEITCLTEEVAQVTVPRIQITSLGPSRSMDLEMKQLQCKLKNATNELTKQSSNLKSLKFELLAKEEHIKEMHEKMSRMERDITMKRHLIEDLKFRQKVNLESNESINEMLENLEKKVKTLTEECSNKKVSIDSLKQRLSVAVKEKSQYEEMYQQTKEELEKKDVKLTFLLSKINETESAMAEIETAASKHLQGLAMQSEQALEGAQKKLLLASEKVEEFTIFVKALVRELQNDIHLIRRQIRELKKMQKNRDACKTSTHKAQTLAASILNISRSDLEEILDTEDEAEIERAKTEAENDKEWLLYIRKLLEGQLPFATYLLEAIMEKINEKKRLVEEYFTVMKDTR

Target ORF information:

RefSeq Version	XM_006067955.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centlein, centrosomal protein (CNTLN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006067955.1

ORF Insert Sequence:

ATGGCGGCGC GGTCGCCGCC CTCACAGCTC CCTCCGCCCC CGGCGCGACA CTTGGGACCC 
TGGTCCCCAC GTGTTGGTCG GGGAGCTGTG GCACACGCAG TGCGCAGCGA GACCTCGGGT 
CTTGAGGGCG CAGCGCGGGA GGGCGTCGTG GACAAAAGCG ATTTACCCTG GCGGGGCGAA 
GAAGGGTCAG AGGGCCAGCG AGGGCCAGCG GGGACAGCTC CGGCTCAGGC TCCCCTCCTC 
AGCGCGCCCA TGGGGTCCAT GCGGCTAGAG GGCATCTCGG TAGAGGAGGC GATGGTGACC 
CGGACGCAGC TGCTGGAGGA AGAGCTGAGC AGCGTAAAGG AGGAGCTGGC CCTGTGTCAG 
GCTGATAAAG AATTCGTATG GTCTTTGTGG AAACGCCTCC AGGTGACAAA TCCAGATGTC 
ACACAAGCAG TCAGTTTGGT TGTGGAAAGA GAAAAACGGA AATCTGAAGC TAAAGACAGA 
AAAGTTTTAG AAATTCTGCA AGTCAAGGAT GCTAAAATAC AAGAATTGGA ACAGAGAGAA 
TCAGGACTAA CACAGGAAAT AAATGACCTT GTAAAACGGA AGATTGCAGT AGATGAAGAA 
AATGATTTCT TAAGGAAAGA ATTCGGTGAC TTGCAGAAGA AATTTAAAGA GAAAAGTCAA 
GAAGTTAAGG ACACTAAGGA GTGTGTGCAG ATTAAGGAAG AGCAAAACAG GCTGGTTATA 
AAAAATCTGA AGGAGGAAAA TGAGAAATTA AGTATCCACT GCGCTGACCT GCTAAATGAG 
CTGGAGAAAC TGAGGAAGCA GGAAGCACAT TGGAGAAAAG AAAAATTTAG CATTGATGCA 
AAAGTAAAGG CCTTTGAAGA CAATTTAATT GAAGCAAGGA AAGAAATTGA AGTATCACAG 
AGCAAATATA ATGCACTATC ATTACAGCTG AATAATAAAC AGACAGAACT TATGCAGAAG 
GATATGGATA TTACCTTGGT CAGGCAAGAG TTGCAGGAGC TACAGAATCT TTACAAACAG 
AACAGTGCAC ATACAGCACA GCAAGCCGAG CTGATCCAGC AGCTTCAGGT TCTGAATATG 
GACACACAGA AAGTACTGAG GAACCAGGAA GATGTTCACA CAGCTGAAAG TATATCGTAT 
CAAAAACTTT ACAATGAATT ACATATTTGT TTTGAAACCA CAAAATCAAG TGAAGCTATG 
CTCCGACAAA GTGTTGTTAC TCTTCAGGAT CAGCTATTTC AAAAAGAGCA AGAAAATGCT 
AAATTAAAAG AAAAGCTTCA GCAATCACAA GAAACACTCT ATCCTTTACC TCGAGAATGT 
GATTCAGACC GCTCAGAACA GGTTTCTCAA CAGCCATCTT TATCAAGCTT AGAAACCTTA 
ATGGTCTCAC AGAAGTCTGA AATTGAGTAT TTACAGGAGA AACTGAAAAT AGCAAATGAA 
AAACTGTCAG AGAACAGATC TACCAACAAG GGTTCCTCAG AGAAGAGCAT CTTGACAAGT 
ACTGAAGGGA AACGTAAGGA ACCACCTGTA AAACGTTCAA GGTCTTTGTC CCCAAAGAGC 
TCTTTCGCAG ACTCGGAGGA ACTTAAGAAG CTGAAGAAAG CTGAAAGAAG GATTGAAAAC 
TTAGAGAAGG CACTACAACT AAAGAACCAA GAAAATGATG AGCTAAGAGA TGCCCATGAA 
AAACGCAAAG AAAGGCTACA GATGTTACAG ACCAACTACA GAGCAGTAAA AGAGCAATTA 
AAACAGTGGG AAGAAGGCAG TGGCATGATG GAAATCAGGA AAGTAAAGAG AGCAGATCCT 
CAACAACTTC AACAAGAAGA TTCTGATGCT GTATGGAATG AACTGGCCTA TTTCAAAAGG 
GAGAACCAGG AGCTAATGAT TCAAAAAATG CATCTTCAAG AAGAACTGGA TAAACTTAAA 
GTACATATGT CTGTTGATAA AGCAACAATA CAAGAATTGA ATACATTTAT GGCAGAGAGA 
AGAGAAGAAC AACTCTTTAG ACACGATGAA GATGATGGGG TCAAGAAAAG TACTCCAGAG 
AAGAATTGGA AAGAAATGTC GAAGCAGACA TTACAGAAGG TCATTGAATT GGAAAATCGG 
CTAAAATCTT TTGAGAAAAG GTCAAGAAAA TTAAAAGAAG GGAATAAAAA TTTAATAAAA 
GAAAATGATT TCCTGAAATC TCTTGTTAAA GAGCAGCAAG AAGATGCAAA GACCAGAGAA 
AAAGAGCTGG AACAATTAAA AAAGGGAAGT AAAGGTGTGG AAAAAGACAA AGCTAAACTT 
GAGGCAAAAA TCAGTGAGCT GGAGATAGAA GTCATTTCCC TAAGGAGACA AGTGGCAGAA 
GCTAATGAGT TGAGAAATGA AAATGAAGAG CTGATGAATT CAATGGAGAA ATTACAGCAT 
TCAGCGGACA AAGCTAAATC TGAGATGGCC ACCACGGAAG CGCGATCTGG ATCTGGGCAA 
TCTGAGTGTA AGACAACCAC TACCAAGGTT AAATTTAAAA CAGCCAAGAA AAAGTGCTCT 
GTGGGTCGTT ACCACACTGT TCTCAATCAC TCCATCAAGG TCATGAGCAA TGTGTTTGAG 
AACCTCAACA AGGACGACTG GGAGGACGTG AGTGAAAGCA GTGATTCTGA AACACAGAAC 
TTACGAAATT TGGGAACAAT TATTGTAGAA ACATCCCAAA AAATAAGTCC AACAGAAGAC 
AGACAAGACC AGAAAGAACG TGACCAAACG GAGGACAGCC AAATGCAAGG AAAAGAAATA 
GTACAGATAT ATCCAAATGT AGAGGACAAG ACCCCAAAGA ATTATTTTCA CAAGAATGCC 
AAAAAATCAG CTCTTCAAAA GAAGAATAGC AGTATTCAAA AGACTTCACA TACAGCAGGT 
CCTACCAGAG TTAACAGAGA AAAGTGGAAA AATATAACTG CCCAGAAATC AAATAGTAAT 
GTTATTTTAT TACGAGAACG GATTGTATCC TTGCAACAGC AAAACAGTGT GCTTTTGAAT 
GCCAAAAAAG CAGCAGAACT GGCTGTTAAA GAATATAAAG AAGTCAACGA CAAGCTCCTG 
CATCAGCAGC AAGTGTCTGA TCAACGATTT CAAACAAGCA GACAGACAAT AAAGAAGCTA 
AATTCGGATT TGGCTGAGCT TCAAAAAGAA AAAGAAGATT TACTAAAGAG AGTAGAATCT 
TCATCTGAAA TCACATGTTT GACTGAAGAA GTTGCCCAGG TAACAGTTCC ACGGATACAG 
ATTACATCAC TTGGCCCTTC AAGGAGCATG GATTTGGAAA TGAAGCAATT GCAGTGTAAA 
CTAAAGAATG CAACTAATGA ACTCACTAAA CAATCTTCAA ACTTGAAGTC TCTGAAATTT 
GAACTCCTAG CAAAAGAAGA ACACATAAAG GAAATGCATG AAAAGATGTC TCGAATGGAG 
AGAGATATAA CCATGAAAAG ACATTTGATA GAGGACTTGA AATTTCGACA GAAAGTAAAT 
TTGGAAAGTA ATGAGAGTAT TAATGAAATG CTAGAAAATC TTGAAAAAAA GGTGAAGACA 
TTAACTGAGG AGTGTTCCAA CAAGAAAGTA TCAATTGATT CACTAAAGCA AAGACTTAGC 
GTTGCTGTAA AAGAGAAGTC TCAGTATGAA GAGATGTATC AGCAAACTAA AGAGGAGTTA 
GAAAAAAAGG ATGTCAAGCT TACTTTCCTC CTGTCAAAAA TAAATGAGAC TGAATCTGCC 
ATGGCAGAGA TTGAAACAGC AGCATCTAAG CATCTTCAAG GATTAGCAAT GCAAAGTGAG 
CAGGCCCTAG AAGGTGCACA GAAGAAACTG CTGCTAGCCA GTGAGAAAGT AGAAGAGTTC 
ACCATATTTG TGAAGGCCTT GGTCAGAGAG TTACAAAATG ACATCCATTT GATTAGGCGA 
CAAATCAGAG AGCTTAAAAA GATGCAGAAA AACAGGGATG CTTGTAAAAC CTCAACCCAT 
AAAGCCCAGA CCTTAGCAGC TTCTATCCTG AACATTTCAC GATCGGACCT AGAGGAAATA 
CTGGACACAG AAGATGAAGC GGAAATTGAA AGGGCAAAGA CTGAGGCCGA AAATGACAAA 
GAATGGCTGT TGTACATTCG GAAACTTCTT GAAGGACAGC TTCCCTTTGC CACATATTTA 
CTAGAAGCAA TAATGGAAAA AATAAATGAA AAAAAGAGAC TAGTTGAAGA ATATTTTACA 
GTTATGAAAG ATACTAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTLN ( XM_025281477.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025137262.1 Bubalus bubalis centlein (CNTLN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu62687
Clone ID Related Accession (Same CDS sequence)	XM_025281477.1
Accession Version	XM_025281477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	centlein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC GGTCGCCGCC CTCACAGCTC CCTCCGCCCC CGGCGCGACA CTTGGGACCC 
TGGTCCCCAC GTGTTGGTCG GGGAGCTGTG GCACACGCAG TGCGCAGCGA GACCTCGGGT 
CTTGAGGGCG CAGCGCGGGA GGGCGTCGTG GACAAAAGCG ATTTACCCTG GCGGGGCGAA 
GAAGGGTCAG AGGGCCAGCG AGGGCCAGCG GGGACAGCTC CGGCTCAGGC TCCCCTCCTC 
AGCGCGCCCA TGGGGTCCAT GCGGCTAGAG GGCATCTCGG TAGAGGAGGC GATGGTGACC 
CGGACGCAGC TGCTGGAGGA AGAGCTGAGC AGCGTAAAGG AGGAGCTGGC CCTGTGTCAG 
GCTGATAAAG AATTCGTATG GTCTTTGTGG AAACGCCTCC AGGTGACAAA TCCAGATGTC 
ACACAAGCAG TCAGTTTGGT TGTGGAAAGA GAAAAACGGA AATCTGAAGC TAAAGACAGA 
AAAGTTTTAG AAATTCTGCA AGTCAAGGAT GCTAAAATAC AAGAATTGGA ACAGAGAGAA 
TCAGGACTAA CACAGGAAAT AAATGACCTT GTAAAACGGA AGATTGCAGT AGATGAAGAA 
AATGATTTCT TAAGGAAAGA ATTCGGTGAC TTGCAGAAGA AATTTAAAGA GAAAAGTCAA 
GAAGTTAAGG ACACTAAGGA GTGTGTGCAG ATTAAGGAAG AGCAAAACAG GCTGGTTATA 
AAAAATCTGA AGGAGGAAAA TGAGAAATTA AGTATCCACT GCGCTGACCT GCTAAATGAG 
CTGGAGAAAC TGAGGAAGCA GGAAGCACAT TGGAGAAAAG AAAAATTTAG CATTGATGCA 
AAAGTAAAGG CCTTTGAAGA CAATTTAATT GAAGCAAGGA AAGAAATTGA AGTATCACAG 
AGCAAATATA ATGCACTATC ATTACAGCTG AATAATAAAC AGACAGAACT TATGCAGAAG 
GATATGGATA TTACCTTGGT CAGGAAGGAG TTGCAGGAGC TACAGAATCT TTACAAACAG 
AACAGTGCAC ATACAGCACA GCAAGCCGAG CTGATCCAGC AGCTTCAGGT TCTGAATATG 
GACACACAGA AAGTACTGAG GAACCAGGAA GATGTTCACA CAGCTGAAAG TATATCGTAT 
CAAAAACTTT ACAATGAATT ACATATTTGT TTTGAAACCA CAAAATCAAG TGAAGCTATG 
CTCCGACAAA GTGTTGTTAC TCTTCAGGAT CAGCTATTTC AAAAAGAGCA AGAAAATGCT 
AAATTAAAAG AAAAGCTTCA GCAATCACAA GAAACACTCT ATCCTTTACC TCGAGAATGT 
GATTCAGACC GCTCAGAACA GGTTTCTCAA CAGCCATCTT TATCAAGCTT AGAAACCTTA 
ATGGTCTCAC AGAAGTCTGA AATTGAGTAT TTACAGGAGA AACTGAAAAT AGCAAATGAA 
AAACTGTCAG AGAACAGATC TACCAACAAG GGTTCCTCAG AGAAGAGCAT CTTGACAAGT 
ACTGAAGGGA AACGTAAGGA ACCACCTGTA AAACGTTCAA GGTCTTTGTC CCCAAAGAGC 
TCTTTCGCAG ACTCGGAGGA ACTTAAGAAG CTGAAGAAAG CTGAAAGAAG GATTGAAAAC 
TTAGAGAAGG CACTACAACT AAAGAACCAA GAAAATGATG AGCTAAGAGA TGCCCATGAA 
AAACGCAAAG AAAGGCTACA GATGTTACAG ACCAACTACA GAGCAGTAAA AGAGCAATTA 
AAACAGTGGG AAGAAGGCAG TGGCATGATG GAAATCAGGA AAGTAAAGAG AGCAGATCCT 
CAACAACTTC AACAAGAAGA TTCTGATGCT GTATGGAATG AACTGGCCTA TTTCAAAAGG 
GAGAACCAGG AGCTAATGAT TCAAAAAATG CATCTTCAAG AAGAACTGGA TAAACTTAAA 
GTACATATGT CTGTTGATAA AGCAACAATA CAAGAATTGA ATACATTTAT GGCAGAGAGA 
AGAGAAGAAC AACTCTTTAG ACACGATGAA GATGATGGGG TCAAGAAAAG TACTCCAGAG 
AAGAATTGGA AAGAAATGTC GAAGCAGACA TTACAGAAGG TCATTGAATT GGAAAATCGG 
CTAAAATCTT TTGAGAAAAG GTCAAGAAAA TTAAAAGAAG GGAATAAAAA TTTAATAAAA 
GAAAATGATT TCCTGAAATC TCTTGTTAAA GAGCAGCAAG AAGATGCAAA GACCAGAGAA 
AAAGAGCTGG AACAATTAAA AAAGGGAAGT AAAGGTGTGG AAAAAGACAA AGCTAAACTT 
GAGGCAAAAA TCAGTGAGCT GGAGATAGAA GTCATTTCCC TAAGGAGACA AGTGGCAGAA 
GCTAATGAGT TGAGAAATGA AAATGAAGAG CTGATGAATT CAATGGAGAA ATTACAGCAT 
TCAGCGGACA AAGCTAAATC TGAGATGGCC ACCACGGAAG CGCGATCTGG ATCTGGGCAA 
TCTGAGTGTA AGACAACCAC TACCAAGGTT AAATTTAAAA CAGCCAAGAA AAAGTGCTCT 
GTGGGTCGTT ACCACACTGT TCTCAATCAC TCCATCAAGG TCATGAGCAA TGTGTTTGAG 
AACCTCAACA AGGACGACTG GGAGGACGTG AGTGAAAGCA GTGATTCTGA AACACAGAAC 
TTACGAAATT TGGGAACAAT TATTGTAGAA ACATCCCAAA AAATAAGTCC AACAGAAGAC 
AGACAAGACC AGAAAGAACG TGACCAAACG GAGGACAGCC AAATGCAAGG AAAAGAAATA 
GTACAGATAT ATCCAAATGT AGAGGACAAG ACCCCAAAGA ATTATTTTCA CAAGAATGCC 
AAAAAATCAG CTCTTCAAAA GAAGAATAGC AGTATTCAAA AGACTTCACA TACAGCAGGT 
CCTACCAGAG TTAACAGAGA AAAGTGGAAA AATATAACTG CCCAGAAATC AAATAGTAAT 
GTTATTTTAT TACGAGAACG GATTGTATCC TTGCAACAGC AAAACAGTGT GCTTTTGAAT 
GCCAAAAAAG CAGCAGAACT GGCTGTTAAA GAATATAAAG AAGTCAACGA CAAGCTCCTG 
CATCAGCAGC AAGTGTCTGA TCAACGATTT CAAACAAGCA GACAGACAAT AAAGAAGCTA 
AATTCGGATT TGGCTGAGCT TCAAAAAGAA AAAGAAGATT TACTAAAGAG AGTAGAATCT 
TCATCTGAAA TCACATGTTT GACTGAAGAA GTTGCCCAGG TAACAGTTCC ACGGATACAG 
ATTACATCAC TTGGCCCTTC AAGGAGCATG GATTTGGAAA TGAAGCAATT GCAGTGTAAA 
CTAAAGAATG CAACTAATGA ACTCACTAAA CAATCTTCAA ACTTGAAGTC TCTGAAATTT 
GAACTCCTAG CAAAAGAAGA ACACATAAAG GAAATGCATG AAAAGATGTC TCGAATGGAG 
AGAGATATAA CCATGAAAAG ACATTTGATA GAGGACTTGA AATTTCGACA GAAAGTAAAT 
TTGGAAAGTA ATGAGAGTAT TAATGAAATG CTAGAAAATC TTGAAAAAAA GGTGAAGACA 
TTAACTGAGG AGTGTTCCAA CAAGAAAGTA TCAATTGATT CACTAAAGCA AAGACTTAGC 
GTTGCTGTAA AAGAGAAGTC TCAGTATGAA GAGATGTATC AGCAAACTAA AGAGGAGTTA 
GAAAAAAAGG ATGTCAAGCT TACTTTCCTC CTGTCAAAAA TAAATGAGAC TGAATCTGCC 
ATGGCAGAGA TTGAAACAGC AGCATCTAAG CATCTTCAAG GATTAGCAAT GCAAAGTGAG 
CAGGCCCTAG AAGGTGCACA GAAGAAACTG CTGCTAGCCA GTGAGAAAGT AGAAGAGTTC 
ACCATATTTG TGAAGGCCTT GGTCAGAGAG TTACAAAATG ACATCCATTT GATTAGGCGA 
CAAATCAGAG AGCTTAAAAA GATGCAGAAA AACAGGGATG CTTGTAAAAC CTCAACCCAT 
AAAGCCCAGA CCTTAGCAGC TTCTATCCTG AACATTTCAC GATCGGACCT AGAGGAAATA 
CTGGACACAG AAGATGAAGC GGAAATTGAA AGGGCAAAGA CTGAGGCCGA AAATGACAAA 
GAATGGCTGT TGTACATTCG GAAACTTCTT GAAGGACAGG TTTGGAGATA TAGCGGTGAA 
CAAGAGACAA AAATCACTGT CCTCATGGAG CTAATACTTC TGGTGATGGA GAACAATAAA 
CAGATAAAAT ATAAAAACTA TGAAAGTTTT TCAACAAACT TGAACGGAAT TATGGGTATG 
TATAATGAAA TATGTGTACT TATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025137262.1
CDS	77..4363
Translation	MAARSPPSQLPPPPARHLGPWSPRVGRGAVAHAVRSETSGLEGAAREGVVDKSDLPWRGEEGSEGQRGPAGTAPAQAPLLSAPMGSMRLEGISVEEAMVTRTQLLEEELSSVKEELALCQADKEFVWSLWKRLQVTNPDVTQAVSLVVEREKRKSEAKDRKVLEILQVKDAKIQELEQRESGLTQEINDLVKRKIAVDEENDFLRKEFGDLQKKFKEKSQEVKDTKECVQIKEEQNRLVIKNLKEENEKLSIHCADLLNELEKLRKQEAHWRKEKFSIDAKVKAFEDNLIEARKEIEVSQSKYNALSLQLNNKQTELMQKDMDITLVRKELQELQNLYKQNSAHTAQQAELIQQLQVLNMDTQKVLRNQEDVHTAESISYQKLYNELHICFETTKSSEAMLRQSVVTLQDQLFQKEQENAKLKEKLQQSQETLYPLPRECDSDRSEQVSQQPSLSSLETLMVSQKSEIEYLQEKLKIANEKLSENRSTNKGSSEKSILTSTEGKRKEPPVKRSRSLSPKSSFADSEELKKLKKAERRIENLEKALQLKNQENDELRDAHEKRKERLQMLQTNYRAVKEQLKQWEEGSGMMEIRKVKRADPQQLQQEDSDAVWNELAYFKRENQELMIQKMHLQEELDKLKVHMSVDKATIQELNTFMAERREEQLFRHDEDDGVKKSTPEKNWKEMSKQTLQKVIELENRLKSFEKRSRKLKEGNKNLIKENDFLKSLVKEQQEDAKTREKELEQLKKGSKGVEKDKAKLEAKISELEIEVISLRRQVAEANELRNENEELMNSMEKLQHSADKAKSEMATTEARSGSGQSECKTTTTKVKFKTAKKKCSVGRYHTVLNHSIKVMSNVFENLNKDDWEDVSESSDSETQNLRNLGTIIVETSQKISPTEDRQDQKERDQTEDSQMQGKEIVQIYPNVEDKTPKNYFHKNAKKSALQKKNSSIQKTSHTAGPTRVNREKWKNITAQKSNSNVILLRERIVSLQQQNSVLLNAKKAAELAVKEYKEVNDKLLHQQQVSDQRFQTSRQTIKKLNSDLAELQKEKEDLLKRVESSSEITCLTEEVAQVTVPRIQITSLGPSRSMDLEMKQLQCKLKNATNELTKQSSNLKSLKFELLAKEEHIKEMHEKMSRMERDITMKRHLIEDLKFRQKVNLESNESINEMLENLEKKVKTLTEECSNKKVSIDSLKQRLSVAVKEKSQYEEMYQQTKEELEKKDVKLTFLLSKINETESAMAEIETAASKHLQGLAMQSEQALEGAQKKLLLASEKVEEFTIFVKALVRELQNDIHLIRRQIRELKKMQKNRDACKTSTHKAQTLAASILNISRSDLEEILDTEDEAEIERAKTEAENDKEWLLYIRKLLEGQVWRYSGEQETKITVLMELILLVMENNKQIKYKNYESFSTNLNGIMGMYNEICVLI

Target ORF information:

RefSeq Version	XM_025281477.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centlein (CNTLN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025281477.1

ORF Insert Sequence:

ATGGCGGCGC GGTCGCCGCC CTCACAGCTC CCTCCGCCCC CGGCGCGACA CTTGGGACCC 
TGGTCCCCAC GTGTTGGTCG GGGAGCTGTG GCACACGCAG TGCGCAGCGA GACCTCGGGT 
CTTGAGGGCG CAGCGCGGGA GGGCGTCGTG GACAAAAGCG ATTTACCCTG GCGGGGCGAA 
GAAGGGTCAG AGGGCCAGCG AGGGCCAGCG GGGACAGCTC CGGCTCAGGC TCCCCTCCTC 
AGCGCGCCCA TGGGGTCCAT GCGGCTAGAG GGCATCTCGG TAGAGGAGGC GATGGTGACC 
CGGACGCAGC TGCTGGAGGA AGAGCTGAGC AGCGTAAAGG AGGAGCTGGC CCTGTGTCAG 
GCTGATAAAG AATTCGTATG GTCTTTGTGG AAACGCCTCC AGGTGACAAA TCCAGATGTC 
ACACAAGCAG TCAGTTTGGT TGTGGAAAGA GAAAAACGGA AATCTGAAGC TAAAGACAGA 
AAAGTTTTAG AAATTCTGCA AGTCAAGGAT GCTAAAATAC AAGAATTGGA ACAGAGAGAA 
TCAGGACTAA CACAGGAAAT AAATGACCTT GTAAAACGGA AGATTGCAGT AGATGAAGAA 
AATGATTTCT TAAGGAAAGA ATTCGGTGAC TTGCAGAAGA AATTTAAAGA GAAAAGTCAA 
GAAGTTAAGG ACACTAAGGA GTGTGTGCAG ATTAAGGAAG AGCAAAACAG GCTGGTTATA 
AAAAATCTGA AGGAGGAAAA TGAGAAATTA AGTATCCACT GCGCTGACCT GCTAAATGAG 
CTGGAGAAAC TGAGGAAGCA GGAAGCACAT TGGAGAAAAG AAAAATTTAG CATTGATGCA 
AAAGTAAAGG CCTTTGAAGA CAATTTAATT GAAGCAAGGA AAGAAATTGA AGTATCACAG 
AGCAAATATA ATGCACTATC ATTACAGCTG AATAATAAAC AGACAGAACT TATGCAGAAG 
GATATGGATA TTACCTTGGT CAGGAAGGAG TTGCAGGAGC TACAGAATCT TTACAAACAG 
AACAGTGCAC ATACAGCACA GCAAGCCGAG CTGATCCAGC AGCTTCAGGT TCTGAATATG 
GACACACAGA AAGTACTGAG GAACCAGGAA GATGTTCACA CAGCTGAAAG TATATCGTAT 
CAAAAACTTT ACAATGAATT ACATATTTGT TTTGAAACCA CAAAATCAAG TGAAGCTATG 
CTCCGACAAA GTGTTGTTAC TCTTCAGGAT CAGCTATTTC AAAAAGAGCA AGAAAATGCT 
AAATTAAAAG AAAAGCTTCA GCAATCACAA GAAACACTCT ATCCTTTACC TCGAGAATGT 
GATTCAGACC GCTCAGAACA GGTTTCTCAA CAGCCATCTT TATCAAGCTT AGAAACCTTA 
ATGGTCTCAC AGAAGTCTGA AATTGAGTAT TTACAGGAGA AACTGAAAAT AGCAAATGAA 
AAACTGTCAG AGAACAGATC TACCAACAAG GGTTCCTCAG AGAAGAGCAT CTTGACAAGT 
ACTGAAGGGA AACGTAAGGA ACCACCTGTA AAACGTTCAA GGTCTTTGTC CCCAAAGAGC 
TCTTTCGCAG ACTCGGAGGA ACTTAAGAAG CTGAAGAAAG CTGAAAGAAG GATTGAAAAC 
TTAGAGAAGG CACTACAACT AAAGAACCAA GAAAATGATG AGCTAAGAGA TGCCCATGAA 
AAACGCAAAG AAAGGCTACA GATGTTACAG ACCAACTACA GAGCAGTAAA AGAGCAATTA 
AAACAGTGGG AAGAAGGCAG TGGCATGATG GAAATCAGGA AAGTAAAGAG AGCAGATCCT 
CAACAACTTC AACAAGAAGA TTCTGATGCT GTATGGAATG AACTGGCCTA TTTCAAAAGG 
GAGAACCAGG AGCTAATGAT TCAAAAAATG CATCTTCAAG AAGAACTGGA TAAACTTAAA 
GTACATATGT CTGTTGATAA AGCAACAATA CAAGAATTGA ATACATTTAT GGCAGAGAGA 
AGAGAAGAAC AACTCTTTAG ACACGATGAA GATGATGGGG TCAAGAAAAG TACTCCAGAG 
AAGAATTGGA AAGAAATGTC GAAGCAGACA TTACAGAAGG TCATTGAATT GGAAAATCGG 
CTAAAATCTT TTGAGAAAAG GTCAAGAAAA TTAAAAGAAG GGAATAAAAA TTTAATAAAA 
GAAAATGATT TCCTGAAATC TCTTGTTAAA GAGCAGCAAG AAGATGCAAA GACCAGAGAA 
AAAGAGCTGG AACAATTAAA AAAGGGAAGT AAAGGTGTGG AAAAAGACAA AGCTAAACTT 
GAGGCAAAAA TCAGTGAGCT GGAGATAGAA GTCATTTCCC TAAGGAGACA AGTGGCAGAA 
GCTAATGAGT TGAGAAATGA AAATGAAGAG CTGATGAATT CAATGGAGAA ATTACAGCAT 
TCAGCGGACA AAGCTAAATC TGAGATGGCC ACCACGGAAG CGCGATCTGG ATCTGGGCAA 
TCTGAGTGTA AGACAACCAC TACCAAGGTT AAATTTAAAA CAGCCAAGAA AAAGTGCTCT 
GTGGGTCGTT ACCACACTGT TCTCAATCAC TCCATCAAGG TCATGAGCAA TGTGTTTGAG 
AACCTCAACA AGGACGACTG GGAGGACGTG AGTGAAAGCA GTGATTCTGA AACACAGAAC 
TTACGAAATT TGGGAACAAT TATTGTAGAA ACATCCCAAA AAATAAGTCC AACAGAAGAC 
AGACAAGACC AGAAAGAACG TGACCAAACG GAGGACAGCC AAATGCAAGG AAAAGAAATA 
GTACAGATAT ATCCAAATGT AGAGGACAAG ACCCCAAAGA ATTATTTTCA CAAGAATGCC 
AAAAAATCAG CTCTTCAAAA GAAGAATAGC AGTATTCAAA AGACTTCACA TACAGCAGGT 
CCTACCAGAG TTAACAGAGA AAAGTGGAAA AATATAACTG CCCAGAAATC AAATAGTAAT 
GTTATTTTAT TACGAGAACG GATTGTATCC TTGCAACAGC AAAACAGTGT GCTTTTGAAT 
GCCAAAAAAG CAGCAGAACT GGCTGTTAAA GAATATAAAG AAGTCAACGA CAAGCTCCTG 
CATCAGCAGC AAGTGTCTGA TCAACGATTT CAAACAAGCA GACAGACAAT AAAGAAGCTA 
AATTCGGATT TGGCTGAGCT TCAAAAAGAA AAAGAAGATT TACTAAAGAG AGTAGAATCT 
TCATCTGAAA TCACATGTTT GACTGAAGAA GTTGCCCAGG TAACAGTTCC ACGGATACAG 
ATTACATCAC TTGGCCCTTC AAGGAGCATG GATTTGGAAA TGAAGCAATT GCAGTGTAAA 
CTAAAGAATG CAACTAATGA ACTCACTAAA CAATCTTCAA ACTTGAAGTC TCTGAAATTT 
GAACTCCTAG CAAAAGAAGA ACACATAAAG GAAATGCATG AAAAGATGTC TCGAATGGAG 
AGAGATATAA CCATGAAAAG ACATTTGATA GAGGACTTGA AATTTCGACA GAAAGTAAAT 
TTGGAAAGTA ATGAGAGTAT TAATGAAATG CTAGAAAATC TTGAAAAAAA GGTGAAGACA 
TTAACTGAGG AGTGTTCCAA CAAGAAAGTA TCAATTGATT CACTAAAGCA AAGACTTAGC 
GTTGCTGTAA AAGAGAAGTC TCAGTATGAA GAGATGTATC AGCAAACTAA AGAGGAGTTA 
GAAAAAAAGG ATGTCAAGCT TACTTTCCTC CTGTCAAAAA TAAATGAGAC TGAATCTGCC 
ATGGCAGAGA TTGAAACAGC AGCATCTAAG CATCTTCAAG GATTAGCAAT GCAAAGTGAG 
CAGGCCCTAG AAGGTGCACA GAAGAAACTG CTGCTAGCCA GTGAGAAAGT AGAAGAGTTC 
ACCATATTTG TGAAGGCCTT GGTCAGAGAG TTACAAAATG ACATCCATTT GATTAGGCGA 
CAAATCAGAG AGCTTAAAAA GATGCAGAAA AACAGGGATG CTTGTAAAAC CTCAACCCAT 
AAAGCCCAGA CCTTAGCAGC TTCTATCCTG AACATTTCAC GATCGGACCT AGAGGAAATA 
CTGGACACAG AAGATGAAGC GGAAATTGAA AGGGCAAAGA CTGAGGCCGA AAATGACAAA 
GAATGGCTGT TGTACATTCG GAAACTTCTT GAAGGACAGG TTTGGAGATA TAGCGGTGAA 
CAAGAGACAA AAATCACTGT CCTCATGGAG CTAATACTTC TGGTGATGGA GAACAATAAA 
CAGATAAAAT ATAAAAACTA TGAAAGTTTT TCAACAAACT TGAACGGAAT TATGGGTATG 
TATAATGAAA TATGTGTACT TATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTLN ( XM_025281478.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025137263.1 Bubalus bubalis centlein (CNTLN), transcript variant X2, mRNA.

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CloneID	OBu62688
Clone ID Related Accession (Same CDS sequence)	XM_025281478.1
Accession Version	XM_025281478.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	centlein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC GGTCGCCGCC CTCACAGCTC CCTCCGCCCC CGGCGCGACA CTTGGGACCC 
TGGTCCCCAC GTGTTGGTCG GGGAGCTGTG GCACACGCAG TGCGCAGCGA GACCTCGGGT 
CTTGAGGGCG CAGCGCGGGA GGGCGTCGTG GACAAAAGCG ATTTACCCTG GCGGGGCGAA 
GAAGGGTCAG AGGGCCAGCG AGGGCCAGCG GGGACAGCTC CGGCTCAGGC TCCCCTCCTC 
AGCGCGCCCA TGGGGTCCAT GCGGCTAGAG GGCATCTCGG TAGAGGAGGC GATGGTGACC 
CGGACGCAGC TGCTGGAGGA AGAGCTGAGC AGCGTAAAGG AGGAGCTGGC CCTGTGTCAG 
GCTGATAAAG AATTCGTATG GTCTTTGTGG AAACGCCTCC AGGTGACAAA TCCAGATGTC 
ACACAAGCAG TCAGTTTGGT TGTGGAAAGA GAAAAACGGA AATCTGAAGC TAAAGACAGA 
AAAGTTTTAG AAATTCTGCA AGTCAAGGAT GCTAAAATAC AAGAATTGGA ACAGAGAGAA 
TCAGGACTAA CACAGGAAAT AAATGACCTT GTAAAACGGA AGATTGCAGT AGATGAAGAA 
AATGATTTCT TAAGGAAAGA ATTCGGTGAC TTGCAGAAGA AATTTAAAGA GAAAAGTCAA 
GAAGTTAAGG ACACTAAGGA GTGTGTGCAG ATTAAGGAAG AGCAAAACAG GCTGGTTATA 
AAAAATCTGA AGGAGGAAAA TGAGAAATTA AGTATCCACT GCGCTGACCT GCTAAATGAG 
CTGGAGAAAC TGAGGAAGCA GGAAGCACAT TGGAGAAAAG AAAAATTTAG CATTGATGCA 
AAAGTAAAGG CCTTTGAAGA CAATTTAATT GAAGCAAGGA AAGAAATTGA AGTATCACAG 
AGCAAATATA ATGCACTATC ATTACAGCTG AATAATAAAC AGACAGAACT TATGCAGAAG 
GATATGGATA TTACCTTGGT CAGGAAGGAG TTGCAGGAGC TACAGAATCT TTACAAACAG 
AACAGTGCAC ATACAGCACA GCAAGCCGAG CTGATCCAGC AGCTTCAGGT TCTGAATATG 
GACACACAGA AAGTACTGAG GAACCAGGAA GATGTTCACA CAGCTGAAAG TATATCGTAT 
CAAAAACTTT ACAATGAATT ACATATTTGT TTTGAAACCA CAAAATCAAG TGAAGCTATG 
CTCCGACAAA GTGTTGTTAC TCTTCAGGAT CAGCTATTTC AAAAAGAGCA AGAAAATGCT 
AAATTAAAAG AAAAGCTTCA GCAATCACAA GAAACACTCT ATCCTTTACC TCGAGAATGT 
GATTCAGACC GCTCAGAACA GGTTTCTCAA CAGCCATCTT TATCAAGCTT AGAAACCTTA 
ATGGTCTCAC AGAAGTCTGA AATTGAGTAT TTACAGGAGA AACTGAAAAT AGCAAATGAA 
AAACTGTCAG AGAACAGATC TACCAACAAG GGTTCCTCAG AGAAGAGCAT CTTGACAAGT 
ACTGAAGGGA AACGTAAGGA ACCACCTGTA AAACGTTCAA GGTCTTTGTC CCCAAAGAGC 
TCTTTCGCAG ACTCGGAGGA ACTTAAGAAG CTGAAGAAAG CTGAAAGAAG GATTGAAAAC 
TTAGAGAAGG CACTACAACT AAAGAACCAA GAAAATGATG AGCTAAGAGA TGCCCATGAA 
AAACGCAAAG AAAGGCTACA GATGTTACAG ACCAACTACA GAGCAGTAAA AGAGCAATTA 
AAACAGTGGG AAGAAGGCAG TGGCATGATG GAAATCAGGA AAGTAAAGAG AGCAGATCCT 
CAACAACTTC AACAAGAAGA TTCTGATGCT GTATGGAATG AACTGGCCTA TTTCAAAAGG 
GAGAACCAGG AGCTAATGAT TCAAAAAATG CATCTTCAAG AAGAACTGGA TAAACTTAAA 
GTACATATGT CTGTTGATAA AGCAACAATA CAAGAATTGA ATACATTTAT GGCAGAGAGA 
AGAGAAGAAC AACTCTTTAG ACACGATGAA GATGATGGGG TCAAGAAAAG TACTCCAGAG 
AAGAATTGGA AAGAAATGTC GAAGCAGACA TTACAGAAGG TCATTGAATT GGAAAATCGG 
CTAAAATCTT TTGAGAAAAG GTCAAGAAAA TTAAAAGAAG GGAATAAAAA TTTAATAAAA 
GAAAATGATT TCCTGAAATC TCTTGTTAAA GAGCAGCAAG AAGATGCAAA GACCAGAGAA 
AAAGAGCTGG AACAATTAAA AAAGGGAAGT AAAGGTGTGG AAAAAGACAA AGCTAAACTT 
GAGGCAAAAA TCAGTGAGCT GGAGATAGAA GTCATTTCCC TAAGGAGACA AGTGGCAGAA 
GCTAATGAGT TGAGAAATGA AAATGAAGAG CTGATGAATT CAATGGAGAA ATTACAGCAT 
TCAGCGGACA AAGCTAAATC TGAGATGGCC ACCACGGAAG CGCGATCTGG ATCTGGGCAA 
TCTGAGTGTA AGACAACCAC TACCAAGGTT AAATTTAAAA CAGCCAAGAA AAAGTGCTCT 
GTGGGTCGTT ACCACACTGT TCTCAATCAC TCCATCAAGG TCATGAGCAA TGTGTTTGAG 
AACCTCAACA AGGACGACTG GGAGGACGTG AGTGAAAGCA GTGATTCTGA AACACAGAAC 
TTACGAAATT TGGGAACAAT TATTGTAGAA ACATCCCAAA AAATAAGTCC AACAGAAGAC 
AGACAAGACC AGAAAGAACG TGACCAAACG GAGGACAGCC AAATGCAAGG AAAAGAAATA 
GTACAGATAT ATCCAAATGT AGAGGACAAG ACCCCAAAGA ATTATTTTCA CAAGAATGCC 
AAAAAATCAG CTCTTCAAAA GAAGAATAGC AGTATTCAAA AGACTTCACA TACAGCAGGT 
CCTACCAGAG TTAACAGAGA AAAGTGGAAA AATATAACTG CCCAGAAATC AAATAGTAAT 
GTTATTTTAT TACGAGAACG GATTGTATCC TTGCAACAGC AAAACAGTGT GCTTTTGAAT 
GCCAAAAAAG CAGCAGAACT GGCTGTTAAA GAATATAAAG AAGTCAACGA CAAGCTCCTG 
CATCAGCAGC AAGTGTCTGA TCAACGATTT CAAACAAGCA GACAGACAAT AAAGAAGCTA 
AATTCGGATT TGGCTGAGCT TCAAAAAGAA AAAGAAGATT TACTAAAGAG AGTAGAATCT 
TCATCTGAAA TCACATGTTT GACTGAAGAA GTTGCCCAGG TAACAGTTCC ACGGATACAG 
ATTACATCAC TTGGCCCTTC AAGGAGCATG GATTTGGAAA TGAAGCAATT GCAGTGTAAA 
CTAAAGAATG CAACTAATGA ACTCACTAAA CAATCTTCAA ACTTGAAGTC TCTGAAATTT 
GAACTCCTAG CAAAAGAAGA ACACATAAAG GAAATGCATG AAAAGATGTC TCGAATGGAG 
AGAGATATAA CCATGAAAAG ACATTTGATA GAGGACTTGA AATTTCGACA GAAAGTAAAT 
TTGGAAAGTA ATGAGAGTAT TAATGAAATG CTAGAAAATC TTGAAAAAAA GGTGAAGACA 
TTAACTGAGG AGTGTTCCAA CAAGAAAGTA TCAATTGATT CACTAAAGCA AAGACTTAGC 
GTTGCTGTAA AAGAGAAGTC TCAGTATGAA GAGATGTATC AGCAAACTAA AGAGGAGTTA 
GAAAAAAAGG ATGTCAAGCT TACTTTCCTC CTGTCAAAAA TAAATGAGAC TGAATCTGCC 
ATGGCAGAGA TTGAAACAGC AGCATCTAAG CATCTTCAAG GATTAGCAAT GCAAAGTGAG 
CAGGCCCTAG AAGGTGCACA GAAGAAACTG CTGCTAGCCA GTGAGAAAGT AGAAGAGTTC 
ACCATATTTG TGAAGGCCTT GGTCAGAGAG TTACAAAATG ACATCCATTT GATTAGGCGA 
CAAATCAGAG AGCTTAAAAA GATGCAGAAA AACAGGGATG CTTGTAAAAC CTCAACCCAT 
AAAGCCCAGA CCTTAGCAGC TTCTATCCTG AACATTTCAC GATCGGACCT AGAGGAAATA 
CTGGACACAG AAGATGAAGC GGAAATTGAA AGGGCAAAGA CTGAGGCCGA AAATGACAAA 
GAATGGCTGT TGTACATTCG GAAACTTCTT GAAGGACAGC TTCCCTTTGC CACATATTTA 
CTAGAAGCAA TAATGGAAAA AATAAATGAA AAAAAGAGAC TAGTTGAAGA ATATTTTACA 
GTTATGAAAG ATACTAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025137263.1
CDS	75..4295
Translation	MAARSPPSQLPPPPARHLGPWSPRVGRGAVAHAVRSETSGLEGAAREGVVDKSDLPWRGEEGSEGQRGPAGTAPAQAPLLSAPMGSMRLEGISVEEAMVTRTQLLEEELSSVKEELALCQADKEFVWSLWKRLQVTNPDVTQAVSLVVEREKRKSEAKDRKVLEILQVKDAKIQELEQRESGLTQEINDLVKRKIAVDEENDFLRKEFGDLQKKFKEKSQEVKDTKECVQIKEEQNRLVIKNLKEENEKLSIHCADLLNELEKLRKQEAHWRKEKFSIDAKVKAFEDNLIEARKEIEVSQSKYNALSLQLNNKQTELMQKDMDITLVRKELQELQNLYKQNSAHTAQQAELIQQLQVLNMDTQKVLRNQEDVHTAESISYQKLYNELHICFETTKSSEAMLRQSVVTLQDQLFQKEQENAKLKEKLQQSQETLYPLPRECDSDRSEQVSQQPSLSSLETLMVSQKSEIEYLQEKLKIANEKLSENRSTNKGSSEKSILTSTEGKRKEPPVKRSRSLSPKSSFADSEELKKLKKAERRIENLEKALQLKNQENDELRDAHEKRKERLQMLQTNYRAVKEQLKQWEEGSGMMEIRKVKRADPQQLQQEDSDAVWNELAYFKRENQELMIQKMHLQEELDKLKVHMSVDKATIQELNTFMAERREEQLFRHDEDDGVKKSTPEKNWKEMSKQTLQKVIELENRLKSFEKRSRKLKEGNKNLIKENDFLKSLVKEQQEDAKTREKELEQLKKGSKGVEKDKAKLEAKISELEIEVISLRRQVAEANELRNENEELMNSMEKLQHSADKAKSEMATTEARSGSGQSECKTTTTKVKFKTAKKKCSVGRYHTVLNHSIKVMSNVFENLNKDDWEDVSESSDSETQNLRNLGTIIVETSQKISPTEDRQDQKERDQTEDSQMQGKEIVQIYPNVEDKTPKNYFHKNAKKSALQKKNSSIQKTSHTAGPTRVNREKWKNITAQKSNSNVILLRERIVSLQQQNSVLLNAKKAAELAVKEYKEVNDKLLHQQQVSDQRFQTSRQTIKKLNSDLAELQKEKEDLLKRVESSSEITCLTEEVAQVTVPRIQITSLGPSRSMDLEMKQLQCKLKNATNELTKQSSNLKSLKFELLAKEEHIKEMHEKMSRMERDITMKRHLIEDLKFRQKVNLESNESINEMLENLEKKVKTLTEECSNKKVSIDSLKQRLSVAVKEKSQYEEMYQQTKEELEKKDVKLTFLLSKINETESAMAEIETAASKHLQGLAMQSEQALEGAQKKLLLASEKVEEFTIFVKALVRELQNDIHLIRRQIRELKKMQKNRDACKTSTHKAQTLAASILNISRSDLEEILDTEDEAEIERAKTEAENDKEWLLYIRKLLEGQLPFATYLLEAIMEKINEKKRLVEEYFTVMKDTR

Target ORF information:

RefSeq Version	XM_025281478.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centlein (CNTLN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025281478.1

ORF Insert Sequence:

ATGGCGGCGC GGTCGCCGCC CTCACAGCTC CCTCCGCCCC CGGCGCGACA CTTGGGACCC 
TGGTCCCCAC GTGTTGGTCG GGGAGCTGTG GCACACGCAG TGCGCAGCGA GACCTCGGGT 
CTTGAGGGCG CAGCGCGGGA GGGCGTCGTG GACAAAAGCG ATTTACCCTG GCGGGGCGAA 
GAAGGGTCAG AGGGCCAGCG AGGGCCAGCG GGGACAGCTC CGGCTCAGGC TCCCCTCCTC 
AGCGCGCCCA TGGGGTCCAT GCGGCTAGAG GGCATCTCGG TAGAGGAGGC GATGGTGACC 
CGGACGCAGC TGCTGGAGGA AGAGCTGAGC AGCGTAAAGG AGGAGCTGGC CCTGTGTCAG 
GCTGATAAAG AATTCGTATG GTCTTTGTGG AAACGCCTCC AGGTGACAAA TCCAGATGTC 
ACACAAGCAG TCAGTTTGGT TGTGGAAAGA GAAAAACGGA AATCTGAAGC TAAAGACAGA 
AAAGTTTTAG AAATTCTGCA AGTCAAGGAT GCTAAAATAC AAGAATTGGA ACAGAGAGAA 
TCAGGACTAA CACAGGAAAT AAATGACCTT GTAAAACGGA AGATTGCAGT AGATGAAGAA 
AATGATTTCT TAAGGAAAGA ATTCGGTGAC TTGCAGAAGA AATTTAAAGA GAAAAGTCAA 
GAAGTTAAGG ACACTAAGGA GTGTGTGCAG ATTAAGGAAG AGCAAAACAG GCTGGTTATA 
AAAAATCTGA AGGAGGAAAA TGAGAAATTA AGTATCCACT GCGCTGACCT GCTAAATGAG 
CTGGAGAAAC TGAGGAAGCA GGAAGCACAT TGGAGAAAAG AAAAATTTAG CATTGATGCA 
AAAGTAAAGG CCTTTGAAGA CAATTTAATT GAAGCAAGGA AAGAAATTGA AGTATCACAG 
AGCAAATATA ATGCACTATC ATTACAGCTG AATAATAAAC AGACAGAACT TATGCAGAAG 
GATATGGATA TTACCTTGGT CAGGAAGGAG TTGCAGGAGC TACAGAATCT TTACAAACAG 
AACAGTGCAC ATACAGCACA GCAAGCCGAG CTGATCCAGC AGCTTCAGGT TCTGAATATG 
GACACACAGA AAGTACTGAG GAACCAGGAA GATGTTCACA CAGCTGAAAG TATATCGTAT 
CAAAAACTTT ACAATGAATT ACATATTTGT TTTGAAACCA CAAAATCAAG TGAAGCTATG 
CTCCGACAAA GTGTTGTTAC TCTTCAGGAT CAGCTATTTC AAAAAGAGCA AGAAAATGCT 
AAATTAAAAG AAAAGCTTCA GCAATCACAA GAAACACTCT ATCCTTTACC TCGAGAATGT 
GATTCAGACC GCTCAGAACA GGTTTCTCAA CAGCCATCTT TATCAAGCTT AGAAACCTTA 
ATGGTCTCAC AGAAGTCTGA AATTGAGTAT TTACAGGAGA AACTGAAAAT AGCAAATGAA 
AAACTGTCAG AGAACAGATC TACCAACAAG GGTTCCTCAG AGAAGAGCAT CTTGACAAGT 
ACTGAAGGGA AACGTAAGGA ACCACCTGTA AAACGTTCAA GGTCTTTGTC CCCAAAGAGC 
TCTTTCGCAG ACTCGGAGGA ACTTAAGAAG CTGAAGAAAG CTGAAAGAAG GATTGAAAAC 
TTAGAGAAGG CACTACAACT AAAGAACCAA GAAAATGATG AGCTAAGAGA TGCCCATGAA 
AAACGCAAAG AAAGGCTACA GATGTTACAG ACCAACTACA GAGCAGTAAA AGAGCAATTA 
AAACAGTGGG AAGAAGGCAG TGGCATGATG GAAATCAGGA AAGTAAAGAG AGCAGATCCT 
CAACAACTTC AACAAGAAGA TTCTGATGCT GTATGGAATG AACTGGCCTA TTTCAAAAGG 
GAGAACCAGG AGCTAATGAT TCAAAAAATG CATCTTCAAG AAGAACTGGA TAAACTTAAA 
GTACATATGT CTGTTGATAA AGCAACAATA CAAGAATTGA ATACATTTAT GGCAGAGAGA 
AGAGAAGAAC AACTCTTTAG ACACGATGAA GATGATGGGG TCAAGAAAAG TACTCCAGAG 
AAGAATTGGA AAGAAATGTC GAAGCAGACA TTACAGAAGG TCATTGAATT GGAAAATCGG 
CTAAAATCTT TTGAGAAAAG GTCAAGAAAA TTAAAAGAAG GGAATAAAAA TTTAATAAAA 
GAAAATGATT TCCTGAAATC TCTTGTTAAA GAGCAGCAAG AAGATGCAAA GACCAGAGAA 
AAAGAGCTGG AACAATTAAA AAAGGGAAGT AAAGGTGTGG AAAAAGACAA AGCTAAACTT 
GAGGCAAAAA TCAGTGAGCT GGAGATAGAA GTCATTTCCC TAAGGAGACA AGTGGCAGAA 
GCTAATGAGT TGAGAAATGA AAATGAAGAG CTGATGAATT CAATGGAGAA ATTACAGCAT 
TCAGCGGACA AAGCTAAATC TGAGATGGCC ACCACGGAAG CGCGATCTGG ATCTGGGCAA 
TCTGAGTGTA AGACAACCAC TACCAAGGTT AAATTTAAAA CAGCCAAGAA AAAGTGCTCT 
GTGGGTCGTT ACCACACTGT TCTCAATCAC TCCATCAAGG TCATGAGCAA TGTGTTTGAG 
AACCTCAACA AGGACGACTG GGAGGACGTG AGTGAAAGCA GTGATTCTGA AACACAGAAC 
TTACGAAATT TGGGAACAAT TATTGTAGAA ACATCCCAAA AAATAAGTCC AACAGAAGAC 
AGACAAGACC AGAAAGAACG TGACCAAACG GAGGACAGCC AAATGCAAGG AAAAGAAATA 
GTACAGATAT ATCCAAATGT AGAGGACAAG ACCCCAAAGA ATTATTTTCA CAAGAATGCC 
AAAAAATCAG CTCTTCAAAA GAAGAATAGC AGTATTCAAA AGACTTCACA TACAGCAGGT 
CCTACCAGAG TTAACAGAGA AAAGTGGAAA AATATAACTG CCCAGAAATC AAATAGTAAT 
GTTATTTTAT TACGAGAACG GATTGTATCC TTGCAACAGC AAAACAGTGT GCTTTTGAAT 
GCCAAAAAAG CAGCAGAACT GGCTGTTAAA GAATATAAAG AAGTCAACGA CAAGCTCCTG 
CATCAGCAGC AAGTGTCTGA TCAACGATTT CAAACAAGCA GACAGACAAT AAAGAAGCTA 
AATTCGGATT TGGCTGAGCT TCAAAAAGAA AAAGAAGATT TACTAAAGAG AGTAGAATCT 
TCATCTGAAA TCACATGTTT GACTGAAGAA GTTGCCCAGG TAACAGTTCC ACGGATACAG 
ATTACATCAC TTGGCCCTTC AAGGAGCATG GATTTGGAAA TGAAGCAATT GCAGTGTAAA 
CTAAAGAATG CAACTAATGA ACTCACTAAA CAATCTTCAA ACTTGAAGTC TCTGAAATTT 
GAACTCCTAG CAAAAGAAGA ACACATAAAG GAAATGCATG AAAAGATGTC TCGAATGGAG 
AGAGATATAA CCATGAAAAG ACATTTGATA GAGGACTTGA AATTTCGACA GAAAGTAAAT 
TTGGAAAGTA ATGAGAGTAT TAATGAAATG CTAGAAAATC TTGAAAAAAA GGTGAAGACA 
TTAACTGAGG AGTGTTCCAA CAAGAAAGTA TCAATTGATT CACTAAAGCA AAGACTTAGC 
GTTGCTGTAA AAGAGAAGTC TCAGTATGAA GAGATGTATC AGCAAACTAA AGAGGAGTTA 
GAAAAAAAGG ATGTCAAGCT TACTTTCCTC CTGTCAAAAA TAAATGAGAC TGAATCTGCC 
ATGGCAGAGA TTGAAACAGC AGCATCTAAG CATCTTCAAG GATTAGCAAT GCAAAGTGAG 
CAGGCCCTAG AAGGTGCACA GAAGAAACTG CTGCTAGCCA GTGAGAAAGT AGAAGAGTTC 
ACCATATTTG TGAAGGCCTT GGTCAGAGAG TTACAAAATG ACATCCATTT GATTAGGCGA 
CAAATCAGAG AGCTTAAAAA GATGCAGAAA AACAGGGATG CTTGTAAAAC CTCAACCCAT 
AAAGCCCAGA CCTTAGCAGC TTCTATCCTG AACATTTCAC GATCGGACCT AGAGGAAATA 
CTGGACACAG AAGATGAAGC GGAAATTGAA AGGGCAAAGA CTGAGGCCGA AAATGACAAA 
GAATGGCTGT TGTACATTCG GAAACTTCTT GAAGGACAGC TTCCCTTTGC CACATATTTA 
CTAGAAGCAA TAATGGAAAA AATAAATGAA AAAAAGAGAC TAGTTGAAGA ATATTTTACA 
GTTATGAAAG ATACTAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTLN ( XM_025281479.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025137264.1 Bubalus bubalis centlein (CNTLN), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu62689
Clone ID Related Accession (Same CDS sequence)	XM_025281479.1
Accession Version	XM_025281479.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	centlein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC GGTCGCCGCC CTCACAGCTC CCTCCGCCCC CGGCGCGACA CTTGGGACCC 
TGGTCCCCAC GTGTTGGTCG GGGAGCTGTG GCACACGCAG TGCGCAGCGA GACCTCGGGT 
CTTGAGGGCG CAGCGCGGGA GGGCGTCGTG GACAAAAGCG ATTTACCCTG GCGGGGCGAA 
GAAGGGTCAG AGGGCCAGCG AGGGCCAGCG GGGACAGCTC CGGCTCAGGC TCCCCTCCTC 
AGCGCGCCCA TGGGGTCCAT GCGGCTAGAG GGCATCTCGG TAGAGGAGGC GATGGTGACC 
CGGACGCAGC TGCTGGAGGA AGAGCTGAGC AGCGTAAAGG AGGAGCTGGC CCTGTGTCAG 
GCTGATAAAG AATTCGTATG GTCTTTGTGG AAACGCCTCC AGGTGACAAA TCCAGATGTC 
ACACAAGCAG TCAGTTTGGT TGTGGAAAGA GAAAAACGGA AATCTGAAGC TAAAGACAGA 
AAAGTTTTAG AAATTCTGCA AGTCAAGGAT GCTAAAATAC AAGAATTGGA ACAGAGAGAA 
TCAGGACTAA CACAGGAAAT AAATGACCTT GTAAAACGGA AGATTGCAGT AGATGAAGAA 
AATGATTTCT TAAGGAAAGA ATTCGGTGAC TTGCAGAAGA AATTTAAAGA GAAAAGTCAA 
GAAGTTAAGG ACACTAAGGA GTGTGTGCAG ATTAAGGAAG AGCAAAACAG GCTGGTTATA 
AAAAATCTGA AGGAGGAAAA TGAGAAATTA AGTATCCACT GCGCTGACCT GCTAAATGAG 
CTGGAGAAAC TGAGGAAGCA GGAAGCACAT TGGAGAAAAG AAAAATTTAG CATTGATGCA 
AAAGTAAAGG CCTTTGAAGA CAATTTAATT GAAGCAAGGA AAGAAATTGA AGTATCACAG 
AGCAAATATA ATGCACTATC ATTACAGCTG AATAATAAAC AGACAGAACT TATGCAGAAG 
GATATGGATA TTACCTTGGT CAGGAAGGAG TTGCAGGAGC TACAGAATCT TTACAAACAG 
AACAGTGCAC ATACAGCACA GCAAGCCGAG CTGATCCAGC AGCTTCAGGT TCTGAATATG 
GACACACAGA AAGTACTGAG GAACCAGGAA GATGTTCACA CAGCTGAAAG TATATCGTAT 
CAAAAACTTT ACAATGAATT ACATATTTGT TTTGAAACCA CAAAATCAAG TGAAGCTATG 
CTCCGACAAA GTGTTGTTAC TCTTCAGGAT CAGCTATTTC AAAAAGAGCA AGAAAATGCT 
AAATTAAAAG AAAAGCTTCA GCAATCACAA GAAACACTCT ATCCTTTACC TCGAGAATGT 
GATTCAGACC GCTCAGAACA GGTTTCTCAA CAGCCATCTT TATCAAGCTT AGAAACCTTA 
ATGGTCTCAC AGAAGTCTGA AATTGAGTAT TTACAGGAGA AACTGAAAAT AGCAAATGAA 
AAACTGTCAG AGAACAGATC TACCAACAAG GGTTCCTCAG AGAAGAGCAT CTTGACAAGT 
ACTGAAGGGA AACGTAAGGA ACCACCTGTA AAACGTTCAA GGTCTTTGTC CCCAAAGAGC 
TCTTTCGCAG ACTCGGAGGA ACTTAAGAAG CTGAAGAAAG CTGAAAGAAG GATTGAAAAC 
TTAGAGAAGG CACTACAACT AAAGAACCAA GAAAATGATG AGCTAAGAGA TGCCCATGAA 
AAACGCAAAG AAAGGCTACA GATGTTACAG ACCAACTACA GAGCAGTAAA AGAGCAATTA 
AAACAGTGGG AAGAAGGCAG TGGCATGATG GAAATCAGGA AAGTAAAGAG AGCAGATCCT 
CAACAACTTC AACAAGAAGA TTCTGATGCT GTATGGAATG AACTGGCCTA TTTCAAAAGG 
GAGAACCAGG AGCTAATGAT TCAAAAAATG CATCTTCAAG AAGAACTGGA TAAACTTAAA 
GTACATATGT CTGTTGATAA AGCAACAATA CAAGAATTGA ATACATTTAT GGCAGAGAGA 
AGAGAAGAAC AACTCTTTAG ACACGATGAA GATGATGGGG TCAAGAAAAG TACTCCAGAG 
AAGAATTGGA AAGAAATGTC GAAGCAGACA TTACAGAAGG TCATTGAATT GGAAAATCGG 
CTAAAATCTT TTGAGAAAAG GTCAAGAAAA TTAAAAGAAG GGAATAAAAA TTTAATAAAA 
GAAAATGATT TCCTGAAATC TCTTGTTAAA GAGCAGCAAG AAGATGCAAA GACCAGAGAA 
AAAGAGCTGG AACAATTAAA AAAGGGAAGT AAAGGTGTGG AAAAAGACAA AGCTAAACTT 
GAGGCAAAAA TCAGTGAGCT GGAGATAGAA GTCATTTCCC TAAGGAGACA AGTGGCAGAA 
GCTAATGAGT TGAGAAATGA AAATGAAGAG CTGATGAATT CAATGGAGAA ATTACAGCAT 
TCAGCGGACA AAGCTAAATC TGAGATGGCC ACCACGGAAG CGCGATCTGG ATCTGGGCAA 
TCTGAGTGTA AGACAACCAC TACCAAGGTT AAATTTAAAA CAGCCAAGAA AAAGTGCTCT 
GTGGGTCGTT ACCACACTGT TCTCAATCAC TCCATCAAGG TCATGAGCAA TGTGTTTGAG 
AACCTCAACA AGGACGACTG GGAGGACGTG AGTGAAAGCA GTGATTCTGA AACACAGAAC 
TTACGAAATT TGGGAACAAT TATTGTAGAA ACATCCCAAA AAATAAGTCC AACAGAAGAC 
AGACAAGACC AGAAAGAACG TGACCAAACG GAGGACAGCC AAATGCAAGG AAAAGAAATA 
GTACAGATAT ATCCAAATGT AGAGGACAAG ACCCCAAAGA ATTATTTTCA CAAGAATGCC 
AAAAAATCAG CTCTTCAAAA GAAGAATAGC AGTATTCAAA AGACTTCACA TACAGCAGGT 
CCTACCAGAG TTAACAGAGA AAAGTGGAAA AATATAACTG CCCAGAAATC AAATAGTAAT 
GTTATTTTAT TACGAGAACG GATTGTATCC TTGCAACAGC AAAACAGTGT GCTTTTGAAT 
GCCAAAAAAG CAGCAGAACT GGCTGTTAAA GAATATAAAG AAGTCAACGA CAAGCTCCTG 
CATCAGCAGC AAGTGTCTGA TCAACGATTT CAAACAAGCA GACAGACAAT AAAGAAGCTA 
AATTCGGATT TGGCTGAGCT TCAAAAAGAA AAAGAAGATT TACTAAAGAG AGTAGAATCT 
TCATCTGAAA TCACATGTTT GACTGAAGAA GTTGCCCAGG TAACAGTTCC ACGGATACAG 
ATTACATCAC TTGGCCCTTC AAGGAGCATG GATTTGGAAA TGAAGCAATT GCAGTGTAAA 
CTAAAGAATG CAACTAATGA ACTCACTAAA CAATCTTCAA ACTTGAAGTC TCTGAAATTT 
GAACTCCTAG CAAAAGAAGA ACACATAAAG GAAATGCATG AAAAGATGTC TCGAATGGAG 
AGAGATATAA CCATGAAAAG ACATTTGATA GAGGACTTGA AATTTCGACA GAAAGTAAAT 
TTGGAAAGTA ATGAGAGTAT TAATGAAATG CTAGAAAATC TTGAAAAAAA GGTGAAGACA 
TTAACTGAGG AGTGTTCCAA CAAGAAAGTA TCAATTGATT CACTAAAGCA AAGACTTAGC 
GTTGCTGTAA AAGAGAAGTC TCAGTATGAA GAGATGTATC AGCAAACTAA AGAGGAGTTA 
GAAAAAAAGG ATGTCAAGCT TACTTTCCTC CTGTCAAAAA TAAATGAGAC TGAATCTGCC 
ATGGCAGAGA TTGAAACAGC AGCATCTAAG CATCTTCAAG GATTAGCAAT GCAAAGTGAG 
CAGGCCCTAG AAGGTGCACA GAAGAAACTG CTGCTAGCCA GTGAGAAAGT AGAAGAGTTC 
ACCATATTTG TGAAGGCCTT GGTCAGAGAG TTACAAAATG ACATCCATTT GATTAGGCGA 
CAAATCAGAG AGCTTAAAAA GATGCAGAAA AACAGGGATG CTTGTAAAAC CTCAACCCAT 
AAAGCCCAGA CCTTAGCAGC TTCTATCCTG AACATTTCAC GATCGGACCT AGAGGAAATA 
CTGGACACAG AAGATGAAGC GGAAATTGAA AGGGCAAAGA CTGAGGCCGA AAATGACAAA 
GAATGGCTGT TGTACATTCG GAAACTTCTT GAAGGACAGC CTACTTTCAT GATACCAGGA 
AAAAGACATA TGTCATTAAA GGAAAAAGCT TCCCTTTGCC ACATATTTAC TAGAAGCAAT 
AATGGAAAAA ATAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025137264.1
CDS	77..4294
Translation	MAARSPPSQLPPPPARHLGPWSPRVGRGAVAHAVRSETSGLEGAAREGVVDKSDLPWRGEEGSEGQRGPAGTAPAQAPLLSAPMGSMRLEGISVEEAMVTRTQLLEEELSSVKEELALCQADKEFVWSLWKRLQVTNPDVTQAVSLVVEREKRKSEAKDRKVLEILQVKDAKIQELEQRESGLTQEINDLVKRKIAVDEENDFLRKEFGDLQKKFKEKSQEVKDTKECVQIKEEQNRLVIKNLKEENEKLSIHCADLLNELEKLRKQEAHWRKEKFSIDAKVKAFEDNLIEARKEIEVSQSKYNALSLQLNNKQTELMQKDMDITLVRKELQELQNLYKQNSAHTAQQAELIQQLQVLNMDTQKVLRNQEDVHTAESISYQKLYNELHICFETTKSSEAMLRQSVVTLQDQLFQKEQENAKLKEKLQQSQETLYPLPRECDSDRSEQVSQQPSLSSLETLMVSQKSEIEYLQEKLKIANEKLSENRSTNKGSSEKSILTSTEGKRKEPPVKRSRSLSPKSSFADSEELKKLKKAERRIENLEKALQLKNQENDELRDAHEKRKERLQMLQTNYRAVKEQLKQWEEGSGMMEIRKVKRADPQQLQQEDSDAVWNELAYFKRENQELMIQKMHLQEELDKLKVHMSVDKATIQELNTFMAERREEQLFRHDEDDGVKKSTPEKNWKEMSKQTLQKVIELENRLKSFEKRSRKLKEGNKNLIKENDFLKSLVKEQQEDAKTREKELEQLKKGSKGVEKDKAKLEAKISELEIEVISLRRQVAEANELRNENEELMNSMEKLQHSADKAKSEMATTEARSGSGQSECKTTTTKVKFKTAKKKCSVGRYHTVLNHSIKVMSNVFENLNKDDWEDVSESSDSETQNLRNLGTIIVETSQKISPTEDRQDQKERDQTEDSQMQGKEIVQIYPNVEDKTPKNYFHKNAKKSALQKKNSSIQKTSHTAGPTRVNREKWKNITAQKSNSNVILLRERIVSLQQQNSVLLNAKKAAELAVKEYKEVNDKLLHQQQVSDQRFQTSRQTIKKLNSDLAELQKEKEDLLKRVESSSEITCLTEEVAQVTVPRIQITSLGPSRSMDLEMKQLQCKLKNATNELTKQSSNLKSLKFELLAKEEHIKEMHEKMSRMERDITMKRHLIEDLKFRQKVNLESNESINEMLENLEKKVKTLTEECSNKKVSIDSLKQRLSVAVKEKSQYEEMYQQTKEELEKKDVKLTFLLSKINETESAMAEIETAASKHLQGLAMQSEQALEGAQKKLLLASEKVEEFTIFVKALVRELQNDIHLIRRQIRELKKMQKNRDACKTSTHKAQTLAASILNISRSDLEEILDTEDEAEIERAKTEAENDKEWLLYIRKLLEGQPTFMIPGKRHMSLKEKASLCHIFTRSNNGKNK

Target ORF information:

RefSeq Version	XM_025281479.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centlein (CNTLN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025281479.1

ORF Insert Sequence:

ATGGCGGCGC GGTCGCCGCC CTCACAGCTC CCTCCGCCCC CGGCGCGACA CTTGGGACCC 
TGGTCCCCAC GTGTTGGTCG GGGAGCTGTG GCACACGCAG TGCGCAGCGA GACCTCGGGT 
CTTGAGGGCG CAGCGCGGGA GGGCGTCGTG GACAAAAGCG ATTTACCCTG GCGGGGCGAA 
GAAGGGTCAG AGGGCCAGCG AGGGCCAGCG GGGACAGCTC CGGCTCAGGC TCCCCTCCTC 
AGCGCGCCCA TGGGGTCCAT GCGGCTAGAG GGCATCTCGG TAGAGGAGGC GATGGTGACC 
CGGACGCAGC TGCTGGAGGA AGAGCTGAGC AGCGTAAAGG AGGAGCTGGC CCTGTGTCAG 
GCTGATAAAG AATTCGTATG GTCTTTGTGG AAACGCCTCC AGGTGACAAA TCCAGATGTC 
ACACAAGCAG TCAGTTTGGT TGTGGAAAGA GAAAAACGGA AATCTGAAGC TAAAGACAGA 
AAAGTTTTAG AAATTCTGCA AGTCAAGGAT GCTAAAATAC AAGAATTGGA ACAGAGAGAA 
TCAGGACTAA CACAGGAAAT AAATGACCTT GTAAAACGGA AGATTGCAGT AGATGAAGAA 
AATGATTTCT TAAGGAAAGA ATTCGGTGAC TTGCAGAAGA AATTTAAAGA GAAAAGTCAA 
GAAGTTAAGG ACACTAAGGA GTGTGTGCAG ATTAAGGAAG AGCAAAACAG GCTGGTTATA 
AAAAATCTGA AGGAGGAAAA TGAGAAATTA AGTATCCACT GCGCTGACCT GCTAAATGAG 
CTGGAGAAAC TGAGGAAGCA GGAAGCACAT TGGAGAAAAG AAAAATTTAG CATTGATGCA 
AAAGTAAAGG CCTTTGAAGA CAATTTAATT GAAGCAAGGA AAGAAATTGA AGTATCACAG 
AGCAAATATA ATGCACTATC ATTACAGCTG AATAATAAAC AGACAGAACT TATGCAGAAG 
GATATGGATA TTACCTTGGT CAGGAAGGAG TTGCAGGAGC TACAGAATCT TTACAAACAG 
AACAGTGCAC ATACAGCACA GCAAGCCGAG CTGATCCAGC AGCTTCAGGT TCTGAATATG 
GACACACAGA AAGTACTGAG GAACCAGGAA GATGTTCACA CAGCTGAAAG TATATCGTAT 
CAAAAACTTT ACAATGAATT ACATATTTGT TTTGAAACCA CAAAATCAAG TGAAGCTATG 
CTCCGACAAA GTGTTGTTAC TCTTCAGGAT CAGCTATTTC AAAAAGAGCA AGAAAATGCT 
AAATTAAAAG AAAAGCTTCA GCAATCACAA GAAACACTCT ATCCTTTACC TCGAGAATGT 
GATTCAGACC GCTCAGAACA GGTTTCTCAA CAGCCATCTT TATCAAGCTT AGAAACCTTA 
ATGGTCTCAC AGAAGTCTGA AATTGAGTAT TTACAGGAGA AACTGAAAAT AGCAAATGAA 
AAACTGTCAG AGAACAGATC TACCAACAAG GGTTCCTCAG AGAAGAGCAT CTTGACAAGT 
ACTGAAGGGA AACGTAAGGA ACCACCTGTA AAACGTTCAA GGTCTTTGTC CCCAAAGAGC 
TCTTTCGCAG ACTCGGAGGA ACTTAAGAAG CTGAAGAAAG CTGAAAGAAG GATTGAAAAC 
TTAGAGAAGG CACTACAACT AAAGAACCAA GAAAATGATG AGCTAAGAGA TGCCCATGAA 
AAACGCAAAG AAAGGCTACA GATGTTACAG ACCAACTACA GAGCAGTAAA AGAGCAATTA 
AAACAGTGGG AAGAAGGCAG TGGCATGATG GAAATCAGGA AAGTAAAGAG AGCAGATCCT 
CAACAACTTC AACAAGAAGA TTCTGATGCT GTATGGAATG AACTGGCCTA TTTCAAAAGG 
GAGAACCAGG AGCTAATGAT TCAAAAAATG CATCTTCAAG AAGAACTGGA TAAACTTAAA 
GTACATATGT CTGTTGATAA AGCAACAATA CAAGAATTGA ATACATTTAT GGCAGAGAGA 
AGAGAAGAAC AACTCTTTAG ACACGATGAA GATGATGGGG TCAAGAAAAG TACTCCAGAG 
AAGAATTGGA AAGAAATGTC GAAGCAGACA TTACAGAAGG TCATTGAATT GGAAAATCGG 
CTAAAATCTT TTGAGAAAAG GTCAAGAAAA TTAAAAGAAG GGAATAAAAA TTTAATAAAA 
GAAAATGATT TCCTGAAATC TCTTGTTAAA GAGCAGCAAG AAGATGCAAA GACCAGAGAA 
AAAGAGCTGG AACAATTAAA AAAGGGAAGT AAAGGTGTGG AAAAAGACAA AGCTAAACTT 
GAGGCAAAAA TCAGTGAGCT GGAGATAGAA GTCATTTCCC TAAGGAGACA AGTGGCAGAA 
GCTAATGAGT TGAGAAATGA AAATGAAGAG CTGATGAATT CAATGGAGAA ATTACAGCAT 
TCAGCGGACA AAGCTAAATC TGAGATGGCC ACCACGGAAG CGCGATCTGG ATCTGGGCAA 
TCTGAGTGTA AGACAACCAC TACCAAGGTT AAATTTAAAA CAGCCAAGAA AAAGTGCTCT 
GTGGGTCGTT ACCACACTGT TCTCAATCAC TCCATCAAGG TCATGAGCAA TGTGTTTGAG 
AACCTCAACA AGGACGACTG GGAGGACGTG AGTGAAAGCA GTGATTCTGA AACACAGAAC 
TTACGAAATT TGGGAACAAT TATTGTAGAA ACATCCCAAA AAATAAGTCC AACAGAAGAC 
AGACAAGACC AGAAAGAACG TGACCAAACG GAGGACAGCC AAATGCAAGG AAAAGAAATA 
GTACAGATAT ATCCAAATGT AGAGGACAAG ACCCCAAAGA ATTATTTTCA CAAGAATGCC 
AAAAAATCAG CTCTTCAAAA GAAGAATAGC AGTATTCAAA AGACTTCACA TACAGCAGGT 
CCTACCAGAG TTAACAGAGA AAAGTGGAAA AATATAACTG CCCAGAAATC AAATAGTAAT 
GTTATTTTAT TACGAGAACG GATTGTATCC TTGCAACAGC AAAACAGTGT GCTTTTGAAT 
GCCAAAAAAG CAGCAGAACT GGCTGTTAAA GAATATAAAG AAGTCAACGA CAAGCTCCTG 
CATCAGCAGC AAGTGTCTGA TCAACGATTT CAAACAAGCA GACAGACAAT AAAGAAGCTA 
AATTCGGATT TGGCTGAGCT TCAAAAAGAA AAAGAAGATT TACTAAAGAG AGTAGAATCT 
TCATCTGAAA TCACATGTTT GACTGAAGAA GTTGCCCAGG TAACAGTTCC ACGGATACAG 
ATTACATCAC TTGGCCCTTC AAGGAGCATG GATTTGGAAA TGAAGCAATT GCAGTGTAAA 
CTAAAGAATG CAACTAATGA ACTCACTAAA CAATCTTCAA ACTTGAAGTC TCTGAAATTT 
GAACTCCTAG CAAAAGAAGA ACACATAAAG GAAATGCATG AAAAGATGTC TCGAATGGAG 
AGAGATATAA CCATGAAAAG ACATTTGATA GAGGACTTGA AATTTCGACA GAAAGTAAAT 
TTGGAAAGTA ATGAGAGTAT TAATGAAATG CTAGAAAATC TTGAAAAAAA GGTGAAGACA 
TTAACTGAGG AGTGTTCCAA CAAGAAAGTA TCAATTGATT CACTAAAGCA AAGACTTAGC 
GTTGCTGTAA AAGAGAAGTC TCAGTATGAA GAGATGTATC AGCAAACTAA AGAGGAGTTA 
GAAAAAAAGG ATGTCAAGCT TACTTTCCTC CTGTCAAAAA TAAATGAGAC TGAATCTGCC 
ATGGCAGAGA TTGAAACAGC AGCATCTAAG CATCTTCAAG GATTAGCAAT GCAAAGTGAG 
CAGGCCCTAG AAGGTGCACA GAAGAAACTG CTGCTAGCCA GTGAGAAAGT AGAAGAGTTC 
ACCATATTTG TGAAGGCCTT GGTCAGAGAG TTACAAAATG ACATCCATTT GATTAGGCGA 
CAAATCAGAG AGCTTAAAAA GATGCAGAAA AACAGGGATG CTTGTAAAAC CTCAACCCAT 
AAAGCCCAGA CCTTAGCAGC TTCTATCCTG AACATTTCAC GATCGGACCT AGAGGAAATA 
CTGGACACAG AAGATGAAGC GGAAATTGAA AGGGCAAAGA CTGAGGCCGA AAATGACAAA 
GAATGGCTGT TGTACATTCG GAAACTTCTT GAAGGACAGC CTACTTTCAT GATACCAGGA 
AAAAGACATA TGTCATTAAA GGAAAAAGCT TCCCTTTGCC ACATATTTAC TAGAAGCAAT 
AATGGAAAAA ATAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTLN ( XM_025281480.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025137265.1 Bubalus bubalis centlein (CNTLN), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu62690
Clone ID Related Accession (Same CDS sequence)	XM_025281480.1
Accession Version	XM_025281480.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	centlein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC GGTCGCCGCC CTCACAGCTC CCTCCGCCCC CGGCGCGACA CTTGGGACCC 
TGGTCCCCAC GTGTTGGTCG GGGAGCTGTG GCACACGCAG TGCGCAGCGA GACCTCGGGT 
CTTGAGGGCG CAGCGCGGGA GGGCGTCGTG GACAAAAGCG ATTTACCCTG GCGGGGCGAA 
GAAGGGTCAG AGGGCCAGCG AGGGCCAGCG GGGACAGCTC CGGCTCAGGC TCCCCTCCTC 
AGCGCGCCCA TGGGGTCCAT GCGGCTAGAG GGCATCTCGG TAGAGGAGGC GATGGTGACC 
CGGACGCAGC TGCTGGAGGA AGAGCTGAGC AGCGTAAAGG AGGAGCTGGC CCTGTGTCAG 
GCTGATAAAG AATTCGTATG GTCTTTGTGG AAACGCCTCC AGGTGACAAA TCCAGATGTC 
ACACAAGCAG TCAGTTTGGT TGTGGAAAGA GAAAAACGGA AATCTGAAGC TAAAGACAGA 
AAAGTTTTAG AAATTCTGCA AGTCAAGGAT GCTAAAATAC AAGAATTGGA ACAGAGAGAA 
TCAGGACTAA CACAGGAAAT AAATGACCTT GTAAAACGGA AGATTGCAGT AGATGAAGAA 
AATGATTTCT TAAGGAAAGA ATTCGGTGAC TTGCAGAAGA AATTTAAAGA GAAAAGTCAA 
GAAGTTAAGG ACACTAAGGA GTGTGTGCAG ATTAAGGAAG AGCAAAACAG GCTGGTTATA 
AAAAATCTGA AGGAGGAAAA TGAGAAATTA AGTATCCACT GCGCTGACCT GCTAAATGAG 
CTGGAGAAAC TGAGGAAGCA GGAAGCACAT TGGAGAAAAG AAAAATTTAG CATTGATGCA 
AAAGTAAAGG CCTTTGAAGA CAATTTAATT GAAGCAAGGA AAGAAATTGA AGTATCACAG 
AGCAAATATA ATGCACTATC ATTACAGCTG AATAATAAAC AGACAGAACT TATGCAGAAG 
GATATGGATA TTACCTTGGT CAGGAAGGAG TTGCAGGAGC TACAGAATCT TTACAAACAG 
AACAGTGCAC ATACAGCACA GCAAGCCGAG CTGATCCAGC AGCTTCAGGT TCTGAATATG 
GACACACAGA AAGTACTGAG GAACCAGGAA GATGTTCACA CAGCTGAAAG TATATCGTAT 
CAAAAAGTTT CTCAACAGCC ATCTTTATCA AGCTTAGAAA CCTTAATGGT CTCACAGAAG 
TCTGAAATTG AGTATTTACA GGAGAAACTG AAAATAGCAA ATGAAAAACT GTCAGAGAAC 
AGATCTACCA ACAAGGGTTC CTCAGAGAAG AGCATCTTGA CAAGTACTGA AGGGAAACGT 
AAGGAACCAC CTGTAAAACG TTCAAGGTCT TTGTCCCCAA AGAGCTCTTT CGCAGACTCG 
GAGGAACTTA AGAAGCTGAA GAAAGCTGAA AGAAGGATTG AAAACTTAGA GAAGGCACTA 
CAACTAAAGA ACCAAGAAAA TGATGAGCTA AGAGATGCCC ATGAAAAACG CAAAGAAAGG 
CTACAGATGT TACAGACCAA CTACAGAGCA GTAAAAGAGC AATTAAAACA GTGGGAAGAA 
GGCAGTGGCA TGATGGAAAT CAGGAAAGTA AAGAGAGCAG ATCCTCAACA ACTTCAACAA 
GAAGATTCTG ATGCTGTATG GAATGAACTG GCCTATTTCA AAAGGGAGAA CCAGGAGCTA 
ATGATTCAAA AAATGCATCT TCAAGAAGAA CTGGATAAAC TTAAAGTACA TATGTCTGTT 
GATAAAGCAA CAATACAAGA ATTGAATACA TTTATGGCAG AGAGAAGAGA AGAACAACTC 
TTTAGACACG ATGAAGATGA TGGGGTCAAG AAAAGTACTC CAGAGAAGAA TTGGAAAGAA 
ATGTCGAAGC AGACATTACA GAAGGTCATT GAATTGGAAA ATCGGCTAAA ATCTTTTGAG 
AAAAGGTCAA GAAAATTAAA AGAAGGGAAT AAAAATTTAA TAAAAGAAAA TGATTTCCTG 
AAATCTCTTG TTAAAGAGCA GCAAGAAGAT GCAAAGACCA GAGAAAAAGA GCTGGAACAA 
TTAAAAAAGG GAAGTAAAGG TGTGGAAAAA GACAAAGCTA AACTTGAGGC AAAAATCAGT 
GAGCTGGAGA TAGAAGTCAT TTCCCTAAGG AGACAAGTGG CAGAAGCTAA TGAGTTGAGA 
AATGAAAATG AAGAGCTGAT GAATTCAATG GAGAAATTAC AGCATTCAGC GGACAAAGCT 
AAATCTGAGA TGGCCACCAC GGAAGCGCGA TCTGGATCTG GGCAATCTGA GTGTAAGACA 
ACCACTACCA AGGTTAAATT TAAAACAGCC AAGAAAAAGT GCTCTGTGGG TCGTTACCAC 
ACTGTTCTCA ATCACTCCAT CAAGGTCATG AGCAATGTGT TTGAGAACCT CAACAAGGAC 
GACTGGGAGG ACGTGAGTGA AAGCAGTGAT TCTGAAACAC AGAACTTACG AAATTTGGGA 
ACAATTATTG TAGAAACATC CCAAAAAATA AGTCCAACAG AAGACAGACA AGACCAGAAA 
GAACGTGACC AAACGGAGGA CAGCCAAATG CAAGGAAAAG AAATAGTACA GATATATCCA 
AATGTAGAGG ACAAGACCCC AAAGAATTAT TTTCACAAGA ATGCCAAAAA ATCAGCTCTT 
CAAAAGAAGA ATAGCAGTAT TCAAAAGACT TCACATACAG CAGGTCCTAC CAGAGTTAAC 
AGAGAAAAGT GGAAAAATAT AACTGCCCAG AAATCAAATA GTAATGTTAT TTTATTACGA 
GAACGGATTG TATCCTTGCA ACAGCAAAAC AGTGTGCTTT TGAATGCCAA AAAAGCAGCA 
GAACTGGCTG TTAAAGAATA TAAAGAAGTC AACGACAAGC TCCTGCATCA GCAGCAAGTG 
TCTGATCAAC GATTTCAAAC AAGCAGACAG ACAATAAAGA AGCTAAATTC GGATTTGGCT 
GAGCTTCAAA AAGAAAAAGA AGATTTACTA AAGAGAGTAG AATCTTCATC TGAAATCACA 
TGTTTGACTG AAGAAGTTGC CCAGGTAACA GTTCCACGGA TACAGATTAC ATCACTTGGC 
CCTTCAAGGA GCATGGATTT GGAAATGAAG CAATTGCAGT GTAAACTAAA GAATGCAACT 
AATGAACTCA CTAAACAATC TTCAAACTTG AAGTCTCTGA AATTTGAACT CCTAGCAAAA 
GAAGAACACA TAAAGGAAAT GCATGAAAAG ATGTCTCGAA TGGAGAGAGA TATAACCATG 
AAAAGACATT TGATAGAGGA CTTGAAATTT CGACAGAAAG TAAATTTGGA AAGTAATGAG 
AGTATTAATG AAATGCTAGA AAATCTTGAA AAAAAGGTGA AGACATTAAC TGAGGAGTGT 
TCCAACAAGA AAGTATCAAT TGATTCACTA AAGCAAAGAC TTAGCGTTGC TGTAAAAGAG 
AAGTCTCAGT ATGAAGAGAT GTATCAGCAA ACTAAAGAGG AGTTAGAAAA AAAGGATGTC 
AAGCTTACTT TCCTCCTGTC AAAAATAAAT GAGACTGAAT CTGCCATGGC AGAGATTGAA 
ACAGCAGCAT CTAAGCATCT TCAAGGATTA GCAATGCAAA GTGAGCAGGC CCTAGAAGGT 
GCACAGAAGA AACTGCTGCT AGCCAGTGAG AAAGTAGAAG AGTTCACCAT ATTTGTGAAG 
GCCTTGGTCA GAGAGTTACA AAATGACATC CATTTGATTA GGCGACAAAT CAGAGAGCTT 
AAAAAGATGC AGAAAAACAG GGATGCTTGT AAAACCTCAA CCCATAAAGC CCAGACCTTA 
GCAGCTTCTA TCCTGAACAT TTCACGATCG GACCTAGAGG AAATACTGGA CACAGAAGAT 
GAAGCGGAAA TTGAAAGGGC AAAGACTGAG GCCGAAAATG ACAAAGAATG GCTGTTGTAC 
ATTCGGAAAC TTCTTGAAGG ACAGGTTTGG AGATATAGCG GTGAACAAGA GACAAAAATC 
ACTGTCCTCA TGGAGCTAAT ACTTCTGGTG ATGGAGAACA ATAAACAGAT AAAATATAAA 
AACTATGAAA GTTTTTCAAC AAACTTGAAC GGAATTATGG GTATGTATAA TGAAATATGT 
GTACTTATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025137265.1
CDS	78..4169
Translation	MAARSPPSQLPPPPARHLGPWSPRVGRGAVAHAVRSETSGLEGAAREGVVDKSDLPWRGEEGSEGQRGPAGTAPAQAPLLSAPMGSMRLEGISVEEAMVTRTQLLEEELSSVKEELALCQADKEFVWSLWKRLQVTNPDVTQAVSLVVEREKRKSEAKDRKVLEILQVKDAKIQELEQRESGLTQEINDLVKRKIAVDEENDFLRKEFGDLQKKFKEKSQEVKDTKECVQIKEEQNRLVIKNLKEENEKLSIHCADLLNELEKLRKQEAHWRKEKFSIDAKVKAFEDNLIEARKEIEVSQSKYNALSLQLNNKQTELMQKDMDITLVRKELQELQNLYKQNSAHTAQQAELIQQLQVLNMDTQKVLRNQEDVHTAESISYQKVSQQPSLSSLETLMVSQKSEIEYLQEKLKIANEKLSENRSTNKGSSEKSILTSTEGKRKEPPVKRSRSLSPKSSFADSEELKKLKKAERRIENLEKALQLKNQENDELRDAHEKRKERLQMLQTNYRAVKEQLKQWEEGSGMMEIRKVKRADPQQLQQEDSDAVWNELAYFKRENQELMIQKMHLQEELDKLKVHMSVDKATIQELNTFMAERREEQLFRHDEDDGVKKSTPEKNWKEMSKQTLQKVIELENRLKSFEKRSRKLKEGNKNLIKENDFLKSLVKEQQEDAKTREKELEQLKKGSKGVEKDKAKLEAKISELEIEVISLRRQVAEANELRNENEELMNSMEKLQHSADKAKSEMATTEARSGSGQSECKTTTTKVKFKTAKKKCSVGRYHTVLNHSIKVMSNVFENLNKDDWEDVSESSDSETQNLRNLGTIIVETSQKISPTEDRQDQKERDQTEDSQMQGKEIVQIYPNVEDKTPKNYFHKNAKKSALQKKNSSIQKTSHTAGPTRVNREKWKNITAQKSNSNVILLRERIVSLQQQNSVLLNAKKAAELAVKEYKEVNDKLLHQQQVSDQRFQTSRQTIKKLNSDLAELQKEKEDLLKRVESSSEITCLTEEVAQVTVPRIQITSLGPSRSMDLEMKQLQCKLKNATNELTKQSSNLKSLKFELLAKEEHIKEMHEKMSRMERDITMKRHLIEDLKFRQKVNLESNESINEMLENLEKKVKTLTEECSNKKVSIDSLKQRLSVAVKEKSQYEEMYQQTKEELEKKDVKLTFLLSKINETESAMAEIETAASKHLQGLAMQSEQALEGAQKKLLLASEKVEEFTIFVKALVRELQNDIHLIRRQIRELKKMQKNRDACKTSTHKAQTLAASILNISRSDLEEILDTEDEAEIERAKTEAENDKEWLLYIRKLLEGQVWRYSGEQETKITVLMELILLVMENNKQIKYKNYESFSTNLNGIMGMYNEICVLI

Target ORF information:

RefSeq Version	XM_025281480.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centlein (CNTLN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025281480.1

ORF Insert Sequence:

ATGGCGGCGC GGTCGCCGCC CTCACAGCTC CCTCCGCCCC CGGCGCGACA CTTGGGACCC 
TGGTCCCCAC GTGTTGGTCG GGGAGCTGTG GCACACGCAG TGCGCAGCGA GACCTCGGGT 
CTTGAGGGCG CAGCGCGGGA GGGCGTCGTG GACAAAAGCG ATTTACCCTG GCGGGGCGAA 
GAAGGGTCAG AGGGCCAGCG AGGGCCAGCG GGGACAGCTC CGGCTCAGGC TCCCCTCCTC 
AGCGCGCCCA TGGGGTCCAT GCGGCTAGAG GGCATCTCGG TAGAGGAGGC GATGGTGACC 
CGGACGCAGC TGCTGGAGGA AGAGCTGAGC AGCGTAAAGG AGGAGCTGGC CCTGTGTCAG 
GCTGATAAAG AATTCGTATG GTCTTTGTGG AAACGCCTCC AGGTGACAAA TCCAGATGTC 
ACACAAGCAG TCAGTTTGGT TGTGGAAAGA GAAAAACGGA AATCTGAAGC TAAAGACAGA 
AAAGTTTTAG AAATTCTGCA AGTCAAGGAT GCTAAAATAC AAGAATTGGA ACAGAGAGAA 
TCAGGACTAA CACAGGAAAT AAATGACCTT GTAAAACGGA AGATTGCAGT AGATGAAGAA 
AATGATTTCT TAAGGAAAGA ATTCGGTGAC TTGCAGAAGA AATTTAAAGA GAAAAGTCAA 
GAAGTTAAGG ACACTAAGGA GTGTGTGCAG ATTAAGGAAG AGCAAAACAG GCTGGTTATA 
AAAAATCTGA AGGAGGAAAA TGAGAAATTA AGTATCCACT GCGCTGACCT GCTAAATGAG 
CTGGAGAAAC TGAGGAAGCA GGAAGCACAT TGGAGAAAAG AAAAATTTAG CATTGATGCA 
AAAGTAAAGG CCTTTGAAGA CAATTTAATT GAAGCAAGGA AAGAAATTGA AGTATCACAG 
AGCAAATATA ATGCACTATC ATTACAGCTG AATAATAAAC AGACAGAACT TATGCAGAAG 
GATATGGATA TTACCTTGGT CAGGAAGGAG TTGCAGGAGC TACAGAATCT TTACAAACAG 
AACAGTGCAC ATACAGCACA GCAAGCCGAG CTGATCCAGC AGCTTCAGGT TCTGAATATG 
GACACACAGA AAGTACTGAG GAACCAGGAA GATGTTCACA CAGCTGAAAG TATATCGTAT 
CAAAAAGTTT CTCAACAGCC ATCTTTATCA AGCTTAGAAA CCTTAATGGT CTCACAGAAG 
TCTGAAATTG AGTATTTACA GGAGAAACTG AAAATAGCAA ATGAAAAACT GTCAGAGAAC 
AGATCTACCA ACAAGGGTTC CTCAGAGAAG AGCATCTTGA CAAGTACTGA AGGGAAACGT 
AAGGAACCAC CTGTAAAACG TTCAAGGTCT TTGTCCCCAA AGAGCTCTTT CGCAGACTCG 
GAGGAACTTA AGAAGCTGAA GAAAGCTGAA AGAAGGATTG AAAACTTAGA GAAGGCACTA 
CAACTAAAGA ACCAAGAAAA TGATGAGCTA AGAGATGCCC ATGAAAAACG CAAAGAAAGG 
CTACAGATGT TACAGACCAA CTACAGAGCA GTAAAAGAGC AATTAAAACA GTGGGAAGAA 
GGCAGTGGCA TGATGGAAAT CAGGAAAGTA AAGAGAGCAG ATCCTCAACA ACTTCAACAA 
GAAGATTCTG ATGCTGTATG GAATGAACTG GCCTATTTCA AAAGGGAGAA CCAGGAGCTA 
ATGATTCAAA AAATGCATCT TCAAGAAGAA CTGGATAAAC TTAAAGTACA TATGTCTGTT 
GATAAAGCAA CAATACAAGA ATTGAATACA TTTATGGCAG AGAGAAGAGA AGAACAACTC 
TTTAGACACG ATGAAGATGA TGGGGTCAAG AAAAGTACTC CAGAGAAGAA TTGGAAAGAA 
ATGTCGAAGC AGACATTACA GAAGGTCATT GAATTGGAAA ATCGGCTAAA ATCTTTTGAG 
AAAAGGTCAA GAAAATTAAA AGAAGGGAAT AAAAATTTAA TAAAAGAAAA TGATTTCCTG 
AAATCTCTTG TTAAAGAGCA GCAAGAAGAT GCAAAGACCA GAGAAAAAGA GCTGGAACAA 
TTAAAAAAGG GAAGTAAAGG TGTGGAAAAA GACAAAGCTA AACTTGAGGC AAAAATCAGT 
GAGCTGGAGA TAGAAGTCAT TTCCCTAAGG AGACAAGTGG CAGAAGCTAA TGAGTTGAGA 
AATGAAAATG AAGAGCTGAT GAATTCAATG GAGAAATTAC AGCATTCAGC GGACAAAGCT 
AAATCTGAGA TGGCCACCAC GGAAGCGCGA TCTGGATCTG GGCAATCTGA GTGTAAGACA 
ACCACTACCA AGGTTAAATT TAAAACAGCC AAGAAAAAGT GCTCTGTGGG TCGTTACCAC 
ACTGTTCTCA ATCACTCCAT CAAGGTCATG AGCAATGTGT TTGAGAACCT CAACAAGGAC 
GACTGGGAGG ACGTGAGTGA AAGCAGTGAT TCTGAAACAC AGAACTTACG AAATTTGGGA 
ACAATTATTG TAGAAACATC CCAAAAAATA AGTCCAACAG AAGACAGACA AGACCAGAAA 
GAACGTGACC AAACGGAGGA CAGCCAAATG CAAGGAAAAG AAATAGTACA GATATATCCA 
AATGTAGAGG ACAAGACCCC AAAGAATTAT TTTCACAAGA ATGCCAAAAA ATCAGCTCTT 
CAAAAGAAGA ATAGCAGTAT TCAAAAGACT TCACATACAG CAGGTCCTAC CAGAGTTAAC 
AGAGAAAAGT GGAAAAATAT AACTGCCCAG AAATCAAATA GTAATGTTAT TTTATTACGA 
GAACGGATTG TATCCTTGCA ACAGCAAAAC AGTGTGCTTT TGAATGCCAA AAAAGCAGCA 
GAACTGGCTG TTAAAGAATA TAAAGAAGTC AACGACAAGC TCCTGCATCA GCAGCAAGTG 
TCTGATCAAC GATTTCAAAC AAGCAGACAG ACAATAAAGA AGCTAAATTC GGATTTGGCT 
GAGCTTCAAA AAGAAAAAGA AGATTTACTA AAGAGAGTAG AATCTTCATC TGAAATCACA 
TGTTTGACTG AAGAAGTTGC CCAGGTAACA GTTCCACGGA TACAGATTAC ATCACTTGGC 
CCTTCAAGGA GCATGGATTT GGAAATGAAG CAATTGCAGT GTAAACTAAA GAATGCAACT 
AATGAACTCA CTAAACAATC TTCAAACTTG AAGTCTCTGA AATTTGAACT CCTAGCAAAA 
GAAGAACACA TAAAGGAAAT GCATGAAAAG ATGTCTCGAA TGGAGAGAGA TATAACCATG 
AAAAGACATT TGATAGAGGA CTTGAAATTT CGACAGAAAG TAAATTTGGA AAGTAATGAG 
AGTATTAATG AAATGCTAGA AAATCTTGAA AAAAAGGTGA AGACATTAAC TGAGGAGTGT 
TCCAACAAGA AAGTATCAAT TGATTCACTA AAGCAAAGAC TTAGCGTTGC TGTAAAAGAG 
AAGTCTCAGT ATGAAGAGAT GTATCAGCAA ACTAAAGAGG AGTTAGAAAA AAAGGATGTC 
AAGCTTACTT TCCTCCTGTC AAAAATAAAT GAGACTGAAT CTGCCATGGC AGAGATTGAA 
ACAGCAGCAT CTAAGCATCT TCAAGGATTA GCAATGCAAA GTGAGCAGGC CCTAGAAGGT 
GCACAGAAGA AACTGCTGCT AGCCAGTGAG AAAGTAGAAG AGTTCACCAT ATTTGTGAAG 
GCCTTGGTCA GAGAGTTACA AAATGACATC CATTTGATTA GGCGACAAAT CAGAGAGCTT 
AAAAAGATGC AGAAAAACAG GGATGCTTGT AAAACCTCAA CCCATAAAGC CCAGACCTTA 
GCAGCTTCTA TCCTGAACAT TTCACGATCG GACCTAGAGG AAATACTGGA CACAGAAGAT 
GAAGCGGAAA TTGAAAGGGC AAAGACTGAG GCCGAAAATG ACAAAGAATG GCTGTTGTAC 
ATTCGGAAAC TTCTTGAAGG ACAGGTTTGG AGATATAGCG GTGAACAAGA GACAAAAATC 
ACTGTCCTCA TGGAGCTAAT ACTTCTGGTG ATGGAGAACA ATAAACAGAT AAAATATAAA 
AACTATGAAA GTTTTTCAAC AAACTTGAAC GGAATTATGG GTATGTATAA TGAAATATGT 
GTACTTATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CNTLN cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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