NLRP1 gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006060029.1	XP_006060091.1	NACHT, LRR and PYD domains-containing protein 1 isoform X5
XM_006060025.2	XP_006060087.2	NACHT, LRR and PYD domains-containing protein 1 isoform X2
XM_006060027.2	XP_006060089.2	NACHT, LRR and PYD domains-containing protein 1 isoform X3
XM_025280776.1	XP_025136561.1	NACHT, LRR and PYD domains-containing protein 1 isoform X6
XM_025280778.1	XP_025136563.1	NACHT, LRR and PYD domains-containing protein 1 isoform X8
XM_006060029.2	XP_006060091.2	NACHT, LRR and PYD domains-containing protein 1 isoform X8
XM_006060028.2	XP_006060090.2	NACHT, LRR and PYD domains-containing protein 1 isoform X8
XM_006060025.1	XP_006060087.1	NACHT, LRR and PYD domains-containing protein 1 isoform X1
XM_025280775.1	XP_025136560.1	NACHT, LRR and PYD domains-containing protein 1 isoform X4
XM_006060028.1	XP_006060090.1	NACHT, LRR and PYD domains-containing protein 1 isoform X4
XM_025280777.1	XP_025136562.1	NACHT, LRR and PYD domains-containing protein 1 isoform X7
XM_025280781.1	XP_025136566.1	NACHT, LRR and PYD domains-containing protein 1 isoform X8
XM_006060026.1	XP_006060088.1	NACHT, LRR and PYD domains-containing protein 1 isoform X2
XM_006060027.1	XP_006060089.1	NACHT, LRR and PYD domains-containing protein 1 isoform X3
XM_025280774.1	XP_025136559.1	NACHT, LRR and PYD domains-containing protein 1 isoform X1
XM_006060026.2	XP_006060088.2	NACHT, LRR and PYD domains-containing protein 1 isoform X5
XM_025280779.1	XP_025136564.1	NACHT, LRR and PYD domains-containing protein 1 isoform X8
XM_025280780.1	XP_025136565.1	NACHT, LRR and PYD domains-containing protein 1 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu16520	XM_006060025.1 Latest version!	Bubalus bubalis NLR family, pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu16521	XM_006060026.1 Latest version!	Bubalus bubalis NLR family, pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu16522	XM_006060027.1 Latest version!	Bubalus bubalis NLR family, pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu16523	XM_006060029.1 Latest version!	Bubalus bubalis NLR family, pyrin domain containing 1 (NLRP1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu16523	XM_006060028.1 Latest version!	Bubalus bubalis NLR family, pyrin domain containing 1 (NLRP1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu62101	XM_025280774.1 Latest version!	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu62102	XM_006060025.2 Latest version!	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu62103	XM_006060027.2 Latest version!	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu62104	XM_025280775.1 Latest version!	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu62105	XM_006060026.2 Latest version!	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu62106	XM_025280776.1 Latest version!	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu62107	XM_025280777.1 Latest version!	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu62108	XM_025280780.1 Latest version!	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu62108	XM_025280781.1 Latest version!	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu62108	XM_025280778.1 Latest version!	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu62108	XM_025280779.1 Latest version!	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu62108	XM_006060029.2 Latest version!	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu62108	XM_006060028.2 Latest version!	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

NLRP1 ( XM_006060025.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006060087.1 Bubalus bubalis NLR family, pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu16520
Clone ID Related Accession (Same CDS sequence)	XM_006060025.1
Accession Version	XM_006060025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784950.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATCC ACACCAAGGG ACCACACATG AACAAGCAAG GGGCATGCAC CAAGACCCTC 
GAAGAGACAG ACTTGAATTC AGCCCTGTAT CCCTCATTCT CCCACTCGCC AAGTGCACCC 
AACCTGGAAT CTCCCAGCTG GCCCACCTCC ACCAAAGTGT TGACATTCTG CAAGCCTAAG 
CAACCAGAGC CCAAGGCCTC ACTGAAACGG ATTTTGAGCC CCCTAGTTTT CGCATCTGGA 
TATAGGAAAG ACCTTTCCCC AAGCATTTAC CAAGGTTTTC CAAGTTCCCC ATATCATGAG 
TCACCAAGCC AAGAGTTACC CAATGACCCC ACATCCACCG CAGTCCTGAG AGTCTGTGAA 
ATCCCACGTC ATTCCTGTCC AGAGCCCAGA GAGAAGGAGG GTCCTGGGAC TGTATGGCCA 
CTGGTTGAAA CATCTGGAAA CAGCTGCACA GGAAGCAGAG AGAGATACCA GGATCAAAGG 
AAAGAGACAC CTTGCTCCAC CTGGTCATGG AAAAATGAAG ATTTGCACCC AAAATTCATG 
CAGTTGCTAC TTCTACACAC TCCTTACCAC AAAGACTATG AGTCCTTGAA CCGGGAAAGC 
TGGGATCACG GGGTGGTTGA GGGGCAAGGG CATTTGATTG AGGTCAGAGA CTTATTTGGC 
TCAGACCTGG GTCCCCAGGA AGGGCCTCAC ACAGTCATAT TGCATGGAGT TGCTGGAATT 
GGGAAGTCCA CACTGGCCAG GCACATAAGA AGAGCCTGGG AGGAGGGCCA GCTATACAGG 
AACCACTTCC AGCATGTCTT CTACTTCAAC TGCAGAGAGC TGGCCATGTC CAAGCTGACA 
AGTCTGGAGG AACTCATCAC AAAAGACCAG TCCACCACTG TGGCTCCCGC TGGGCAGGTC 
CAGTCTCAGC CGGAGCAGCT GCTCTTCATC CTCGATGGTT TAGATGAACT GGAATGGGTC 
TCAGAGGAGC AGAGGGCAGA GCTCTGTCTG CACCGGAGCC AGCAGCAGCC CGTGCAGACA 
CTGCTGGGCA GTTTGGTGGA GAAAACCATA TTTCCAGAGG CATCCTTGCT GATCACAGCT 
CGGACTGCAG CTCTGCAGAA GTTCATTCCT TCTTTGAAGC AGCCATGTTG GGTGGAGGTC 
CTAGGGTTCT CTGAGTCTTC CAGGAAGGAC TATTTCTATG AATATTTCAC AGAGGAAAGC 
CAAGCAACTA GAGCCTTTAA CGTGGTTGAA TCAAACCAAG CACTCTTGAC CATGTGTCTC 
ACGCCCTTGG TGTCCTGGCT GGCCTGCACT TGCCTGAAGC AGCAGATGGA GGCAGGGGAA 
GAACTCTCAC TGAGGTCACA GACCATGACA GCCCTCTATC TGCGCTACCT TGCCCAAGCT 
ATCCAAGCTC AGCCCCTGGG GACTCAGCTG AGGAGCTTCT GTACTCTTGC TGCTGAGGGT 
ATCTGGCAAG GAAAGACTCT GTTCACTCCA GGGGACCTCA AGAAGCACGG ATTAGATTGG 
GTCATCAACT CCACTCCCTT AAGGATGGAT GTTCTTCAAA AGCACCCCAC GTCTCTGAAC 
TACAGCTTCA TTCACCTGTG TTTCCAGGAG TTCTTTGCAG CAGTGTCCTT TGCCTTAGGG 
GACATGGGGG ACAAAAGTGA TCATCCTAAC AGCACTGGAA GCATGGAAAA GTTGGTAGAA 
GTATATGGGA TCAATAACCT GTTTGGGGCA CCAACCGTGC ATTTCCTATC CGGCCTTTTG 
AGTGAGCAAG GAGCGATAGA GATGGAGAAC ATTTTCCAAT GCAAGCTGTC TTGGGAGAGA 
AAGCGGGATT CACTGCTGCA GTGGGCTGAA TTGGAACTCG GGCGTGAGCA TTACTCTCTG 
CAATTGTTTC ACTGTTTGTA CGAGATTCAG GACGAGGAGT TCCTGACACA AGCGATGGCC 
CATTTCCAAG GAACAAGAGT GTGCATCCGA ACCAGCATGG AGCTGCTCGT GTTCACTTTC 
TGCATTAGAT TCTGCAGCCA CGTGCGGCAG CTTCAACTGA ATGAGGGCAG ACAACATCCA 
GGAGCGTGCA GGCCCACCGA CACAGTGCTG TTGAGCTGGG TCCCGGCCAC AGAGGCCTGC 
TGGCAGGTTC TCTGCTCCGT TCTCCAGGTC ACTGGAAACC TGAAGGAACT GGACCTGAGT 
GGAAACATCT TGAGCCGCTC TGCAGTACAG AGTCTTGATG AGGCCCTGAG ATGCCCTGGG 
TGTCAGCTAG AGACCCTGCG GCTGGCCAGC TGCGGCCTCA CAGCTGAGAG CTGCAAGGAC 
ATTGCCTCTG GGCTGAGCTC CAGCCAAACC CTGATGCAAT TGGAATTGAG CTTCAACACA 
CTCCTGGACG CAGGAGCTGA GCACCTTTGC CAGGGCCTGA GACAGCCAAC CTGCAAGCTG 
CAGCGACTGT TGCTGGCAGG CTGCGGCCTC ACATCTGGCT GCTGCCAGGA CCTGGCCTCC 
ATGCTCAGGG TCAGCCCCAG CCTGATGGAG CTAAACCTGC TGCAGAATGA CCTGGACAAC 
CTCGGCGTGA GGCTGCTCTG TGAAGGACTC AGGCACCCTT CCTGCCAGCT CATATGCCTA 
TGGCTGGACC AGACTCAGCT GAGCGAGGAG GTGATGGAGA TGCTGAAGGC CCTGGAGAGG 
GAGAAGCCTC AGCTGCTAGT CTCCGGGATA TGGAAGCCAA GTCTGAGGAT CCTTCACGAG 
GGCCCAGGTG GAGCAGAGAT GAGCGATACA TCCTCCCTCA AGCGGCAGAG ACAAGCATCA 
GAGGGCAGCT CTCCTCAAGC AGCACAGGTG AGACTCTTCT GTCCGTCTTC TCCTGCCCCT 
CTGGAGGACA AGCACATGGA GTCTCTGGGG ACCGAGAATG ACCTCTGGGG CCCCACAGGG 
CCTGTGGCTA CCCAGGCAGT TGACGAAGAG AGGAGCCTGT ACCGCGTTCA CTTGCCCGTG 
GCCGGCTTCT ACCACTGGCC CAACACGGGT CTCCGCTTTG AGGTGAGAGG GCCAGTGACC 
GTTGAAATTG AATTCTGTGC CTGGGACCAG TTCCTGAACA GGAATTTCCC ACAGCACAGC 
TGGCTGGCTG CAGGGCCTCT GTTTGACATC AAGGCTGAAC CAGGCGCTGT GGCAGCCGTG 
TTCCTCCCTC ACTTTGTGGC CTTCCGAGGG AACGATGTGG ACATCTCCCA GTTTCAAGTG 
GCCCACTTCA AAGAGGAGGG GATGCTCCTG GAGAAGCCAG CCAGGGTGAC GCCATGTTAC 
GCTGTCCTGG AAAACCCCAG CTTCTCGCCC ATGGGAGTTC TACTGAGGAT GGTCCAAACT 
GCCCTGCGCT TCATTCCCAT CACCTCCACT GTGTTGCTCT ACCATTACCT CCAGCCTGAA 
GAAGTTACCT TCCACCTCTA CCTGATCCCC AGCGATTGCT CCATTCAGAA GGCCATAGAC 
AATGAAGAAA AGAAGTTCCA GTTTGTGCGA ATTCACAAGC CACCCCCGCT GACCCCACTC 
TACATGGGCT CCCGCTACAT TGTGTCTGGT TCAGAGAAGC TGGAAATACT GCCCGAGGAA 
TTGGAGCTCT GCTATCGGAG CCCCAGAGAA TCTCAGCTGT TCTCTGAGTT CTACGTTCGC 
CATTTGAGGT CAGGGATCAG GTTGCAAATA AGAAGCAAGA AAGATGGGAC TGTGGTGTGG 
GAAGCCTTGG TGAAGCCAGG AGATCTCAGA CGGGCAGTTA CTCCAGTTCC TCCGGGCCTC 
AGAGCCTCAC CTGACTCCCC AGCTGCCCTG AGATTGAGGC ACTTTGTGGA CCGATATCGG 
GAGCAGCTGG TGGCTCGAGT GACATCCGTG GACCCTATCC TGGACAAGTT GCATGGACAG 
GTGCTGAGCG AAGAACAGTA TGAGGGGGTG CGGGCAGAGA CCACTGCACC AGGCCAGATG 
CGGAAGCTGT TTGGTTTCAG CCGATCCTGG AACTGGGCCT GCAAAGATCG CCTCTACCAA 
GCCCTGAAGG AGACCCATCC TCACCTAATC ATGGAACTCT GGGAGAAGTG GGGCAGTGAA 
GAGACCAGGC CGGGCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006060087.1
CDS	10..4050
Translation	MAIHTKGPHMNKQGACTKTLEETDLNSALYPSFSHSPSAPNLESPSWPTSTKVLTFCKPKQPEPKASLKRILSPLVFASGYRKDLSPSIYQGFPSSPYHESPSQELPNDPTSTAVLRVCEIPRHSCPEPREKEGPGTVWPLVETSGNSCTGSRERYQDQRKETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGPQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELAMSKLTSLEELITKDQSTTVAPAGQVQSQPEQLLFILDGLDELEWVSEEQRAELCLHRSQQQPVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFNVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLRSQTMTALYLRYLAQAIQAQPLGTQLRSFCTLAAEGIWQGKTLFTPGDLKKHGLDWVINSTPLRMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDMGDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAIEMENIFQCKLSWERKRDSLLQWAELELGREHYSLQLFHCLYEIQDEEFLTQAMAHFQGTRVCIRTSMELLVFTFCIRFCSHVRQLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSGNILSRSAVQSLDEALRCPGCQLETLRLASCGLTAESCKDIASGLSSSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDNLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVMEMLKALEREKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPLEDKHMESLGTENDLWGPTGPVATQAVDEERSLYRVHLPVAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQHSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKKFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDGTVVWEALVKPGDLRRAVTPVPPGLRASPDSPAALRLRHFVDRYREQLVARVTSVDPILDKLHGQVLSEEQYEGVRAETTAPGQMRKLFGFSRSWNWACKDRLYQALKETHPHLIMELWEKWGSEETRPGS

Target ORF information:

RefSeq Version	XM_006060025.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family, pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006060025.1

ORF Insert Sequence:

ATGGCCATCC ACACCAAGGG ACCACACATG AACAAGCAAG GGGCATGCAC CAAGACCCTC 
GAAGAGACAG ACTTGAATTC AGCCCTGTAT CCCTCATTCT CCCACTCGCC AAGTGCACCC 
AACCTGGAAT CTCCCAGCTG GCCCACCTCC ACCAAAGTGT TGACATTCTG CAAGCCTAAG 
CAACCAGAGC CCAAGGCCTC ACTGAAACGG ATTTTGAGCC CCCTAGTTTT CGCATCTGGA 
TATAGGAAAG ACCTTTCCCC AAGCATTTAC CAAGGTTTTC CAAGTTCCCC ATATCATGAG 
TCACCAAGCC AAGAGTTACC CAATGACCCC ACATCCACCG CAGTCCTGAG AGTCTGTGAA 
ATCCCACGTC ATTCCTGTCC AGAGCCCAGA GAGAAGGAGG GTCCTGGGAC TGTATGGCCA 
CTGGTTGAAA CATCTGGAAA CAGCTGCACA GGAAGCAGAG AGAGATACCA GGATCAAAGG 
AAAGAGACAC CTTGCTCCAC CTGGTCATGG AAAAATGAAG ATTTGCACCC AAAATTCATG 
CAGTTGCTAC TTCTACACAC TCCTTACCAC AAAGACTATG AGTCCTTGAA CCGGGAAAGC 
TGGGATCACG GGGTGGTTGA GGGGCAAGGG CATTTGATTG AGGTCAGAGA CTTATTTGGC 
TCAGACCTGG GTCCCCAGGA AGGGCCTCAC ACAGTCATAT TGCATGGAGT TGCTGGAATT 
GGGAAGTCCA CACTGGCCAG GCACATAAGA AGAGCCTGGG AGGAGGGCCA GCTATACAGG 
AACCACTTCC AGCATGTCTT CTACTTCAAC TGCAGAGAGC TGGCCATGTC CAAGCTGACA 
AGTCTGGAGG AACTCATCAC AAAAGACCAG TCCACCACTG TGGCTCCCGC TGGGCAGGTC 
CAGTCTCAGC CGGAGCAGCT GCTCTTCATC CTCGATGGTT TAGATGAACT GGAATGGGTC 
TCAGAGGAGC AGAGGGCAGA GCTCTGTCTG CACCGGAGCC AGCAGCAGCC CGTGCAGACA 
CTGCTGGGCA GTTTGGTGGA GAAAACCATA TTTCCAGAGG CATCCTTGCT GATCACAGCT 
CGGACTGCAG CTCTGCAGAA GTTCATTCCT TCTTTGAAGC AGCCATGTTG GGTGGAGGTC 
CTAGGGTTCT CTGAGTCTTC CAGGAAGGAC TATTTCTATG AATATTTCAC AGAGGAAAGC 
CAAGCAACTA GAGCCTTTAA CGTGGTTGAA TCAAACCAAG CACTCTTGAC CATGTGTCTC 
ACGCCCTTGG TGTCCTGGCT GGCCTGCACT TGCCTGAAGC AGCAGATGGA GGCAGGGGAA 
GAACTCTCAC TGAGGTCACA GACCATGACA GCCCTCTATC TGCGCTACCT TGCCCAAGCT 
ATCCAAGCTC AGCCCCTGGG GACTCAGCTG AGGAGCTTCT GTACTCTTGC TGCTGAGGGT 
ATCTGGCAAG GAAAGACTCT GTTCACTCCA GGGGACCTCA AGAAGCACGG ATTAGATTGG 
GTCATCAACT CCACTCCCTT AAGGATGGAT GTTCTTCAAA AGCACCCCAC GTCTCTGAAC 
TACAGCTTCA TTCACCTGTG TTTCCAGGAG TTCTTTGCAG CAGTGTCCTT TGCCTTAGGG 
GACATGGGGG ACAAAAGTGA TCATCCTAAC AGCACTGGAA GCATGGAAAA GTTGGTAGAA 
GTATATGGGA TCAATAACCT GTTTGGGGCA CCAACCGTGC ATTTCCTATC CGGCCTTTTG 
AGTGAGCAAG GAGCGATAGA GATGGAGAAC ATTTTCCAAT GCAAGCTGTC TTGGGAGAGA 
AAGCGGGATT CACTGCTGCA GTGGGCTGAA TTGGAACTCG GGCGTGAGCA TTACTCTCTG 
CAATTGTTTC ACTGTTTGTA CGAGATTCAG GACGAGGAGT TCCTGACACA AGCGATGGCC 
CATTTCCAAG GAACAAGAGT GTGCATCCGA ACCAGCATGG AGCTGCTCGT GTTCACTTTC 
TGCATTAGAT TCTGCAGCCA CGTGCGGCAG CTTCAACTGA ATGAGGGCAG ACAACATCCA 
GGAGCGTGCA GGCCCACCGA CACAGTGCTG TTGAGCTGGG TCCCGGCCAC AGAGGCCTGC 
TGGCAGGTTC TCTGCTCCGT TCTCCAGGTC ACTGGAAACC TGAAGGAACT GGACCTGAGT 
GGAAACATCT TGAGCCGCTC TGCAGTACAG AGTCTTGATG AGGCCCTGAG ATGCCCTGGG 
TGTCAGCTAG AGACCCTGCG GCTGGCCAGC TGCGGCCTCA CAGCTGAGAG CTGCAAGGAC 
ATTGCCTCTG GGCTGAGCTC CAGCCAAACC CTGATGCAAT TGGAATTGAG CTTCAACACA 
CTCCTGGACG CAGGAGCTGA GCACCTTTGC CAGGGCCTGA GACAGCCAAC CTGCAAGCTG 
CAGCGACTGT TGCTGGCAGG CTGCGGCCTC ACATCTGGCT GCTGCCAGGA CCTGGCCTCC 
ATGCTCAGGG TCAGCCCCAG CCTGATGGAG CTAAACCTGC TGCAGAATGA CCTGGACAAC 
CTCGGCGTGA GGCTGCTCTG TGAAGGACTC AGGCACCCTT CCTGCCAGCT CATATGCCTA 
TGGCTGGACC AGACTCAGCT GAGCGAGGAG GTGATGGAGA TGCTGAAGGC CCTGGAGAGG 
GAGAAGCCTC AGCTGCTAGT CTCCGGGATA TGGAAGCCAA GTCTGAGGAT CCTTCACGAG 
GGCCCAGGTG GAGCAGAGAT GAGCGATACA TCCTCCCTCA AGCGGCAGAG ACAAGCATCA 
GAGGGCAGCT CTCCTCAAGC AGCACAGGTG AGACTCTTCT GTCCGTCTTC TCCTGCCCCT 
CTGGAGGACA AGCACATGGA GTCTCTGGGG ACCGAGAATG ACCTCTGGGG CCCCACAGGG 
CCTGTGGCTA CCCAGGCAGT TGACGAAGAG AGGAGCCTGT ACCGCGTTCA CTTGCCCGTG 
GCCGGCTTCT ACCACTGGCC CAACACGGGT CTCCGCTTTG AGGTGAGAGG GCCAGTGACC 
GTTGAAATTG AATTCTGTGC CTGGGACCAG TTCCTGAACA GGAATTTCCC ACAGCACAGC 
TGGCTGGCTG CAGGGCCTCT GTTTGACATC AAGGCTGAAC CAGGCGCTGT GGCAGCCGTG 
TTCCTCCCTC ACTTTGTGGC CTTCCGAGGG AACGATGTGG ACATCTCCCA GTTTCAAGTG 
GCCCACTTCA AAGAGGAGGG GATGCTCCTG GAGAAGCCAG CCAGGGTGAC GCCATGTTAC 
GCTGTCCTGG AAAACCCCAG CTTCTCGCCC ATGGGAGTTC TACTGAGGAT GGTCCAAACT 
GCCCTGCGCT TCATTCCCAT CACCTCCACT GTGTTGCTCT ACCATTACCT CCAGCCTGAA 
GAAGTTACCT TCCACCTCTA CCTGATCCCC AGCGATTGCT CCATTCAGAA GGCCATAGAC 
AATGAAGAAA AGAAGTTCCA GTTTGTGCGA ATTCACAAGC CACCCCCGCT GACCCCACTC 
TACATGGGCT CCCGCTACAT TGTGTCTGGT TCAGAGAAGC TGGAAATACT GCCCGAGGAA 
TTGGAGCTCT GCTATCGGAG CCCCAGAGAA TCTCAGCTGT TCTCTGAGTT CTACGTTCGC 
CATTTGAGGT CAGGGATCAG GTTGCAAATA AGAAGCAAGA AAGATGGGAC TGTGGTGTGG 
GAAGCCTTGG TGAAGCCAGG AGATCTCAGA CGGGCAGTTA CTCCAGTTCC TCCGGGCCTC 
AGAGCCTCAC CTGACTCCCC AGCTGCCCTG AGATTGAGGC ACTTTGTGGA CCGATATCGG 
GAGCAGCTGG TGGCTCGAGT GACATCCGTG GACCCTATCC TGGACAAGTT GCATGGACAG 
GTGCTGAGCG AAGAACAGTA TGAGGGGGTG CGGGCAGAGA CCACTGCACC AGGCCAGATG 
CGGAAGCTGT TTGGTTTCAG CCGATCCTGG AACTGGGCCT GCAAAGATCG CCTCTACCAA 
GCCCTGAAGG AGACCCATCC TCACCTAATC ATGGAACTCT GGGAGAAGTG GGGCAGTGAA 
GAGACCAGGC CGGGCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_006060026.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006060088.1 Bubalus bubalis NLR family, pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu16521
Clone ID Related Accession (Same CDS sequence)	XM_006060026.1
Accession Version	XM_006060026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784950.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATCC ACACCAAGGG ACCACACATG AACAAGCAAG GGGCATGCAC CAAGACCCTC 
GAAGAGACAG ACTTGAATTC AGCCCTGTAT CCCTCATTCT CCCACTCGCC AAGTGCACCC 
AACCTGGAAT CTCCCAGCTG GCCCACCTCC ACCAAAGTGT TGACATTCTG CAAGCCTAAG 
CAACCAGAGC CCAAGGCCTC ACTGAAACGG ATTTTGAGCC CCCTAGTTTT CGCATCTGGA 
TATAGGAAAG ACCTTTCCCC AAGCATTTAC CAAGGTTTTC CAAGTTCCCC ATATCATGAG 
TCACCAAGCC AAGAGTTACC CAATGACCCC ACATCCACCG CAGTCCTGAG AGTCTGTGAA 
ATCCCACGTC ATTCCTGTCC AGAGCCCAGA GAGAAGGAGG GTCCTGGGAC TGTATGGCCA 
CTGGTTGAAA CATCTGGAAA CAGCTGCACA GGAAGCAGAG AGAGATACCA GGATCAAAGG 
AAAGAGACAC CTTGCTCCAC CTGGTCATGG AAAAATGAAG ATTTGCACCC AAAATTCATG 
CAGTTGCTAC TTCTACACAC TCCTTACCAC AAAGACTATG AGTCCTTGAA CCGGGAAAGC 
TGGGATCACG GGGTGGTTGA GGGGCAAGGG CATTTGATTG AGGTCAGAGA CTTATTTGGC 
TCAGACCTGG GTCCCCAGGA AGGGCCTCAC ACAGTCATAT TGCATGGAGT TGCTGGAATT 
GGGAAGTCCA CACTGGCCAG GCACATAAGA AGAGCCTGGG AGGAGGGCCA GCTATACAGG 
AACCACTTCC AGCATGTCTT CTACTTCAAC TGCAGAGAGC TGGCCATGTC CAAGCTGACA 
AGTCTGGAGG AACTCATCAC AAAAGACCAG TCCACCACTG TGGCTCCCGC TGGGCAGGTC 
CAGTCTCAGC CGGAGCAGCT GCTCTTCATC CTCGATGGTT TAGATGAACT GGAATGGGTC 
TCAGAGGAGC AGAGGGCAGA GCTCTGTCTG CACCGGAGCC AGCAGCAGCC CGTGCAGACA 
CTGCTGGGCA GTTTGGTGGA GAAAACCATA TTTCCAGAGG CATCCTTGCT GATCACAGCT 
CGGACTGCAG CTCTGCAGAA GTTCATTCCT TCTTTGAAGC AGCCATGTTG GGTGGAGGTC 
CTAGGGTTCT CTGAGTCTTC CAGGAAGGAC TATTTCTATG AATATTTCAC AGAGGAAAGC 
CAAGCAACTA GAGCCTTTAA CGTGGTTGAA TCAAACCAAG CACTCTTGAC CATGTGTCTC 
ACGCCCTTGG TGTCCTGGCT GGCCTGCACT TGCCTGAAGC AGCAGATGGA GGCAGGGGAA 
GAACTCTCAC TGAGGTCACA GACCATGACA GCCCTCTATC TGCGCTACCT TGCCCAAGCT 
ATCCAAGCTC AGCCCCTGGG GACTCAGCTG AGGAGCTTCT GTACTCTTGC TGCTGAGGGT 
ATCTGGCAAG GAAAGACTCT GTTCACTCCA GGGGACCTCA AGAAGCACGG ATTAGATTGG 
GTCATCAACT CCACTCCCTT AAGGATGGAT GTTCTTCAAA AGCACCCCAC GTCTCTGAAC 
TACAGCTTCA TTCACCTGTG TTTCCAGGAG TTCTTTGCAG CAGTGTCCTT TGCCTTAGGG 
GACATGGGGG ACAAAAGTGA TCATCCTAAC AGCACTGGAA GCATGGAAAA GTTGGTAGAA 
GTATATGGGA TCAATAACCT GTTTGGGGCA CCAACCGTGC ATTTCCTATC CGGCCTTTTG 
AGTGAGCAAG GAGCGATAGA GATGGAGAAC ATTTTCCAAT GCAAGCTGTC TTGGGAGAGA 
AAGCGGGATT CACTGCTGCA GTGGGCTGAA TTGGAACTCG GGCGTGAGCA TTACTCTCTG 
CAATTGTTTC ACTGTTTGTA CGAGATTCAG GACGAGGAGT TCCTGACACA AGCGATGGCC 
CATTTCCAAG GAACAAGAGT GTGCATCCGA ACCAGCATGG AGCTGCTCGT GTTCACTTTC 
TGCATTAGAT TCTGCAGCCA CGTGCGGCAG CTTCAACTGA ATGAGGGCAG ACAACATCCA 
GGAGCGTGCA GGCCCACCGA CACAGTGCTG TTGAGCTGGG TCCCGGCCAC AGAGGCCTGC 
TGGCAGGTTC TCTGCTCCGT TCTCCAGGTC ACTGGAAACC TGAAGGAACT GGACCTGAGT 
GGAAACATCT TGAGCCGCTC TGCAGTACAG AGTCTTGATG AGGCCCTGAG ATGCCCTGGG 
TGTCAGCTAG AGACCCTGCG GCTGGCCAGC TGCGGCCTCA CAGCTGAGAG CTGCAAGGAC 
ATTGCCTCTG GGCTGAGCTC CAGCCAAACC CTGATGCAAT TGGAATTGAG CTTCAACACA 
CTCCTGGACG CAGGAGCTGA GCACCTTTGC CAGGGCCTGA GACAGCCAAC CTGCAAGCTG 
CAGCGACTGT TGCTGGACCA GACTCAGCTG AGCGAGGAGG TGATGGAGAT GCTGAAGGCC 
CTGGAGAGGG AGAAGCCTCA GCTGCTAGTC TCCGGGATAT GGAAGCCAAG TCTGAGGATC 
CTTCACGAGG GCCCAGGTGG AGCAGAGATG AGCGATACAT CCTCCCTCAA GCGGCAGAGA 
CAAGCATCAG AGGGCAGCTC TCCTCAAGCA GCACAGGTGA GACTCTTCTG TCCGTCTTCT 
CCTGCCCCTC TGGAGGACAA GCACATGGAG TCTCTGGGGA CCGAGAATGA CCTCTGGGGC 
CCCACAGGGC CTGTGGCTAC CCAGGCAGTT GACGAAGAGA GGAGCCTGTA CCGCGTTCAC 
TTGCCCGTGG CCGGCTTCTA CCACTGGCCC AACACGGGTC TCCGCTTTGA GGTGAGAGGG 
CCAGTGACCG TTGAAATTGA ATTCTGTGCC TGGGACCAGT TCCTGAACAG GAATTTCCCA 
CAGCACAGCT GGCTGGCTGC AGGGCCTCTG TTTGACATCA AGGCTGAACC AGGCGCTGTG 
GCAGCCGTGT TCCTCCCTCA CTTTGTGGCC TTCCGAGGGA ACGATGTGGA CATCTCCCAG 
TTTCAAGTGG CCCACTTCAA AGAGGAGGGG ATGCTCCTGG AGAAGCCAGC CAGGGTGACG 
CCATGTTACG CTGTCCTGGA AAACCCCAGC TTCTCGCCCA TGGGAGTTCT ACTGAGGATG 
GTCCAAACTG CCCTGCGCTT CATTCCCATC ACCTCCACTG TGTTGCTCTA CCATTACCTC 
CAGCCTGAAG AAGTTACCTT CCACCTCTAC CTGATCCCCA GCGATTGCTC CATTCAGAAG 
GCCATAGACA ATGAAGAAAA GAAGTTCCAG TTTGTGCGAA TTCACAAGCC ACCCCCGCTG 
ACCCCACTCT ACATGGGCTC CCGCTACATT GTGTCTGGTT CAGAGAAGCT GGAAATACTG 
CCCGAGGAAT TGGAGCTCTG CTATCGGAGC CCCAGAGAAT CTCAGCTGTT CTCTGAGTTC 
TACGTTCGCC ATTTGAGGTC AGGGATCAGG TTGCAAATAA GAAGCAAGAA AGATGGGACT 
GTGGTGTGGG AAGCCTTGGT GAAGCCAGGA GATCTCAGAC GGGCAGTTAC TCCAGTTCCT 
CCGGGCCTCA GAGCCTCACC TGACTCCCCA GCTGCCCTGA GATTGAGGCA CTTTGTGGAC 
CGATATCGGG AGCAGCTGGT GGCTCGAGTG ACATCCGTGG ACCCTATCCT GGACAAGTTG 
CATGGACAGG TGCTGAGCGA AGAACAGTAT GAGGGGGTGC GGGCAGAGAC CACTGCACCA 
GGCCAGATGC GGAAGCTGTT TGGTTTCAGC CGATCCTGGA ACTGGGCCTG CAAAGATCGC 
CTCTACCAAG CCCTGAAGGA GACCCATCCT CACCTAATCA TGGAACTCTG GGAGAAGTGG 
GGCAGTGAAG AGACCAGGCC GGGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006060088.1
CDS	10..3879
Translation	MAIHTKGPHMNKQGACTKTLEETDLNSALYPSFSHSPSAPNLESPSWPTSTKVLTFCKPKQPEPKASLKRILSPLVFASGYRKDLSPSIYQGFPSSPYHESPSQELPNDPTSTAVLRVCEIPRHSCPEPREKEGPGTVWPLVETSGNSCTGSRERYQDQRKETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGPQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELAMSKLTSLEELITKDQSTTVAPAGQVQSQPEQLLFILDGLDELEWVSEEQRAELCLHRSQQQPVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFNVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLRSQTMTALYLRYLAQAIQAQPLGTQLRSFCTLAAEGIWQGKTLFTPGDLKKHGLDWVINSTPLRMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDMGDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAIEMENIFQCKLSWERKRDSLLQWAELELGREHYSLQLFHCLYEIQDEEFLTQAMAHFQGTRVCIRTSMELLVFTFCIRFCSHVRQLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSGNILSRSAVQSLDEALRCPGCQLETLRLASCGLTAESCKDIASGLSSSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLDQTQLSEEVMEMLKALEREKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPLEDKHMESLGTENDLWGPTGPVATQAVDEERSLYRVHLPVAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQHSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKKFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDGTVVWEALVKPGDLRRAVTPVPPGLRASPDSPAALRLRHFVDRYREQLVARVTSVDPILDKLHGQVLSEEQYEGVRAETTAPGQMRKLFGFSRSWNWACKDRLYQALKETHPHLIMELWEKWGSEETRPGS

Target ORF information:

RefSeq Version	XM_006060026.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family, pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006060026.1

ORF Insert Sequence:

ATGGCCATCC ACACCAAGGG ACCACACATG AACAAGCAAG GGGCATGCAC CAAGACCCTC 
GAAGAGACAG ACTTGAATTC AGCCCTGTAT CCCTCATTCT CCCACTCGCC AAGTGCACCC 
AACCTGGAAT CTCCCAGCTG GCCCACCTCC ACCAAAGTGT TGACATTCTG CAAGCCTAAG 
CAACCAGAGC CCAAGGCCTC ACTGAAACGG ATTTTGAGCC CCCTAGTTTT CGCATCTGGA 
TATAGGAAAG ACCTTTCCCC AAGCATTTAC CAAGGTTTTC CAAGTTCCCC ATATCATGAG 
TCACCAAGCC AAGAGTTACC CAATGACCCC ACATCCACCG CAGTCCTGAG AGTCTGTGAA 
ATCCCACGTC ATTCCTGTCC AGAGCCCAGA GAGAAGGAGG GTCCTGGGAC TGTATGGCCA 
CTGGTTGAAA CATCTGGAAA CAGCTGCACA GGAAGCAGAG AGAGATACCA GGATCAAAGG 
AAAGAGACAC CTTGCTCCAC CTGGTCATGG AAAAATGAAG ATTTGCACCC AAAATTCATG 
CAGTTGCTAC TTCTACACAC TCCTTACCAC AAAGACTATG AGTCCTTGAA CCGGGAAAGC 
TGGGATCACG GGGTGGTTGA GGGGCAAGGG CATTTGATTG AGGTCAGAGA CTTATTTGGC 
TCAGACCTGG GTCCCCAGGA AGGGCCTCAC ACAGTCATAT TGCATGGAGT TGCTGGAATT 
GGGAAGTCCA CACTGGCCAG GCACATAAGA AGAGCCTGGG AGGAGGGCCA GCTATACAGG 
AACCACTTCC AGCATGTCTT CTACTTCAAC TGCAGAGAGC TGGCCATGTC CAAGCTGACA 
AGTCTGGAGG AACTCATCAC AAAAGACCAG TCCACCACTG TGGCTCCCGC TGGGCAGGTC 
CAGTCTCAGC CGGAGCAGCT GCTCTTCATC CTCGATGGTT TAGATGAACT GGAATGGGTC 
TCAGAGGAGC AGAGGGCAGA GCTCTGTCTG CACCGGAGCC AGCAGCAGCC CGTGCAGACA 
CTGCTGGGCA GTTTGGTGGA GAAAACCATA TTTCCAGAGG CATCCTTGCT GATCACAGCT 
CGGACTGCAG CTCTGCAGAA GTTCATTCCT TCTTTGAAGC AGCCATGTTG GGTGGAGGTC 
CTAGGGTTCT CTGAGTCTTC CAGGAAGGAC TATTTCTATG AATATTTCAC AGAGGAAAGC 
CAAGCAACTA GAGCCTTTAA CGTGGTTGAA TCAAACCAAG CACTCTTGAC CATGTGTCTC 
ACGCCCTTGG TGTCCTGGCT GGCCTGCACT TGCCTGAAGC AGCAGATGGA GGCAGGGGAA 
GAACTCTCAC TGAGGTCACA GACCATGACA GCCCTCTATC TGCGCTACCT TGCCCAAGCT 
ATCCAAGCTC AGCCCCTGGG GACTCAGCTG AGGAGCTTCT GTACTCTTGC TGCTGAGGGT 
ATCTGGCAAG GAAAGACTCT GTTCACTCCA GGGGACCTCA AGAAGCACGG ATTAGATTGG 
GTCATCAACT CCACTCCCTT AAGGATGGAT GTTCTTCAAA AGCACCCCAC GTCTCTGAAC 
TACAGCTTCA TTCACCTGTG TTTCCAGGAG TTCTTTGCAG CAGTGTCCTT TGCCTTAGGG 
GACATGGGGG ACAAAAGTGA TCATCCTAAC AGCACTGGAA GCATGGAAAA GTTGGTAGAA 
GTATATGGGA TCAATAACCT GTTTGGGGCA CCAACCGTGC ATTTCCTATC CGGCCTTTTG 
AGTGAGCAAG GAGCGATAGA GATGGAGAAC ATTTTCCAAT GCAAGCTGTC TTGGGAGAGA 
AAGCGGGATT CACTGCTGCA GTGGGCTGAA TTGGAACTCG GGCGTGAGCA TTACTCTCTG 
CAATTGTTTC ACTGTTTGTA CGAGATTCAG GACGAGGAGT TCCTGACACA AGCGATGGCC 
CATTTCCAAG GAACAAGAGT GTGCATCCGA ACCAGCATGG AGCTGCTCGT GTTCACTTTC 
TGCATTAGAT TCTGCAGCCA CGTGCGGCAG CTTCAACTGA ATGAGGGCAG ACAACATCCA 
GGAGCGTGCA GGCCCACCGA CACAGTGCTG TTGAGCTGGG TCCCGGCCAC AGAGGCCTGC 
TGGCAGGTTC TCTGCTCCGT TCTCCAGGTC ACTGGAAACC TGAAGGAACT GGACCTGAGT 
GGAAACATCT TGAGCCGCTC TGCAGTACAG AGTCTTGATG AGGCCCTGAG ATGCCCTGGG 
TGTCAGCTAG AGACCCTGCG GCTGGCCAGC TGCGGCCTCA CAGCTGAGAG CTGCAAGGAC 
ATTGCCTCTG GGCTGAGCTC CAGCCAAACC CTGATGCAAT TGGAATTGAG CTTCAACACA 
CTCCTGGACG CAGGAGCTGA GCACCTTTGC CAGGGCCTGA GACAGCCAAC CTGCAAGCTG 
CAGCGACTGT TGCTGGACCA GACTCAGCTG AGCGAGGAGG TGATGGAGAT GCTGAAGGCC 
CTGGAGAGGG AGAAGCCTCA GCTGCTAGTC TCCGGGATAT GGAAGCCAAG TCTGAGGATC 
CTTCACGAGG GCCCAGGTGG AGCAGAGATG AGCGATACAT CCTCCCTCAA GCGGCAGAGA 
CAAGCATCAG AGGGCAGCTC TCCTCAAGCA GCACAGGTGA GACTCTTCTG TCCGTCTTCT 
CCTGCCCCTC TGGAGGACAA GCACATGGAG TCTCTGGGGA CCGAGAATGA CCTCTGGGGC 
CCCACAGGGC CTGTGGCTAC CCAGGCAGTT GACGAAGAGA GGAGCCTGTA CCGCGTTCAC 
TTGCCCGTGG CCGGCTTCTA CCACTGGCCC AACACGGGTC TCCGCTTTGA GGTGAGAGGG 
CCAGTGACCG TTGAAATTGA ATTCTGTGCC TGGGACCAGT TCCTGAACAG GAATTTCCCA 
CAGCACAGCT GGCTGGCTGC AGGGCCTCTG TTTGACATCA AGGCTGAACC AGGCGCTGTG 
GCAGCCGTGT TCCTCCCTCA CTTTGTGGCC TTCCGAGGGA ACGATGTGGA CATCTCCCAG 
TTTCAAGTGG CCCACTTCAA AGAGGAGGGG ATGCTCCTGG AGAAGCCAGC CAGGGTGACG 
CCATGTTACG CTGTCCTGGA AAACCCCAGC TTCTCGCCCA TGGGAGTTCT ACTGAGGATG 
GTCCAAACTG CCCTGCGCTT CATTCCCATC ACCTCCACTG TGTTGCTCTA CCATTACCTC 
CAGCCTGAAG AAGTTACCTT CCACCTCTAC CTGATCCCCA GCGATTGCTC CATTCAGAAG 
GCCATAGACA ATGAAGAAAA GAAGTTCCAG TTTGTGCGAA TTCACAAGCC ACCCCCGCTG 
ACCCCACTCT ACATGGGCTC CCGCTACATT GTGTCTGGTT CAGAGAAGCT GGAAATACTG 
CCCGAGGAAT TGGAGCTCTG CTATCGGAGC CCCAGAGAAT CTCAGCTGTT CTCTGAGTTC 
TACGTTCGCC ATTTGAGGTC AGGGATCAGG TTGCAAATAA GAAGCAAGAA AGATGGGACT 
GTGGTGTGGG AAGCCTTGGT GAAGCCAGGA GATCTCAGAC GGGCAGTTAC TCCAGTTCCT 
CCGGGCCTCA GAGCCTCACC TGACTCCCCA GCTGCCCTGA GATTGAGGCA CTTTGTGGAC 
CGATATCGGG AGCAGCTGGT GGCTCGAGTG ACATCCGTGG ACCCTATCCT GGACAAGTTG 
CATGGACAGG TGCTGAGCGA AGAACAGTAT GAGGGGGTGC GGGCAGAGAC CACTGCACCA 
GGCCAGATGC GGAAGCTGTT TGGTTTCAGC CGATCCTGGA ACTGGGCCTG CAAAGATCGC 
CTCTACCAAG CCCTGAAGGA GACCCATCCT CACCTAATCA TGGAACTCTG GGAGAAGTGG 
GGCAGTGAAG AGACCAGGCC GGGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_006060027.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006060089.1 Bubalus bubalis NLR family, pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.

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CloneID	OBu16522
Clone ID Related Accession (Same CDS sequence)	XM_006060027.1
Accession Version	XM_006060027.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784950.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATCC ACACCAAGGG ACCACACATG AACAAGCAAG GGGCATGCAC CAAGACCCTC 
GAAGAGACAG ACTTGAATTC AGCCCTGTAT CCCTCATTCT CCCACTCGCC AAGTGCACCC 
AACCTGGAAT CTCCCAGCTG GCCCACCTCC ACCAAAGTGT TGACATTCTG CAAGCCTAAG 
CAACCAGAGC CCAAGGCCTC ACTGAAACGG ATTTTGAGCC CCCTAGTTTT CGCATCTGGA 
TATAGGAAAG ACCTTTCCCC AAGCATTTAC CAAGGTTTTC CAAGTTCCCC ATATCATGAG 
TCACCAAGCC AAGAGTTACC CAATGACCCC ACATCCACCG CAGTCCTGAG AGTCTGTGAA 
ATCCCACGTC ATTCCTGTCC AGAGCCCAGA GAGAAGGAGG GTCCTGGGAC TGTATGGCCA 
CTGGTTGAAA CATCTGGAAA CAGCTGCACA GGAAGCAGAG AGAGATACCA GGATCAAAGG 
AAAGAGACAC CTTGCTCCAC CTGGTCATGG AAAAATGAAG ATTTGCACCC AAAATTCATG 
CAGTTGCTAC TTCTACACAC TCCTTACCAC AAAGACTATG AGTCCTTGAA CCGGGAAAGC 
TGGGATCACG GGGTGGTTGA GGGGCAAGGG CATTTGATTG AGGTCAGAGA CTTATTTGGC 
TCAGACCTGG GTCCCCAGGA AGGGCCTCAC ACAGTCATAT TGCATGGAGT TGCTGGAATT 
GGGAAGTCCA CACTGGCCAG GCACATAAGA AGAGCCTGGG AGGAGGGCCA GCTATACAGG 
AACCACTTCC AGCATGTCTT CTACTTCAAC TGCAGAGAGC TGGCCATGTC CAAGCTGACA 
AGTCTGGAGG AACTCATCAC AAAAGACCAG TCCACCACTG TGGCTCCCGC TGGGCAGGTC 
CAGTCTCAGC CGGAGCAGCT GCTCTTCATC CTCGATGGTT TAGATGAACT GGAATGGGTC 
TCAGAGGAGC AGAGGGCAGA GCTCTGTCTG CACCGGAGCC AGCAGCAGCC CGTGCAGACA 
CTGCTGGGCA GTTTGGTGGA GAAAACCATA TTTCCAGAGG CATCCTTGCT GATCACAGCT 
CGGACTGCAG CTCTGCAGAA GTTCATTCCT TCTTTGAAGC AGCCATGTTG GGTGGAGGTC 
CTAGGGTTCT CTGAGTCTTC CAGGAAGGAC TATTTCTATG AATATTTCAC AGAGGAAAGC 
CAAGCAACTA GAGCCTTTAA CGTGGTTGAA TCAAACCAAG CACTCTTGAC CATGTGTCTC 
ACGCCCTTGG TGTCCTGGCT GGCCTGCACT TGCCTGAAGC AGCAGATGGA GGCAGGGGAA 
GAACTCTCAC TGAGGTCACA GACCATGACA GCCCTCTATC TGCGCTACCT TGCCCAAGCT 
ATCCAAGCTC AGCCCCTGGG GACTCAGCTG AGGAGCTTCT GTACTCTTGC TGCTGAGGGT 
ATCTGGCAAG GAAAGACTCT GTTCACTCCA GGGGACCTCA AGAAGCACGG ATTAGATTGG 
GTCATCAACT CCACTCCCTT AAGGATGGAT GTTCTTCAAA AGCACCCCAC GTCTCTGAAC 
TACAGCTTCA TTCACCTGTG TTTCCAGGAG TTCTTTGCAG CAGTGTCCTT TGCCTTAGGG 
GACATGGGGG ACAAAAGTGA TCATCCTAAC AGCACTGGAA GCATGGAAAA GTTGGTAGAA 
GTATATGGGA TCAATAACCT GTTTGGGGCA CCAACCGTGC ATTTCCTATC CGGCCTTTTG 
AGTGAGCAAG GAGCGATAGA GATGGAGAAC ATTTTCCAAT GCAAGCTGTC TTGGGAGAGA 
AAGCGGGATT CACTGCTGCA GTGGGCTGAA TTGGAACTCG GGCGTGAGCA TTACTCTCTG 
CAATTGTTTC ACTGTTTGTA CGAGATTCAG GACGAGGAGT TCCTGACACA AGCGATGGCC 
CATTTCCAAG GAACAAGAGT GTGCATCCGA ACCAGCATGG AGCTGCTCGT GTTCACTTTC 
TGCATTAGAT TCTGCAGCCA CGTGCGGCAG CTTCAACTGA ATGAGGGCAG ACAACATCCA 
GGAGCGTGCA GGCCCACCGA CACAGTGCTG TTGAGCTGGG TCCCGGCCAC AGAGGCCTGC 
TGGCAGGTTC TCTGCTCCGT TCTCCAGGTC ACTGGAAACC TGAAGGAACT GGACCTGAGT 
GGAAACATCT TGAGCCGCTC TGCAGTACAG AGTCTTGATG AGGCCCTGAG ATGCCCTGGG 
TGTCAGCTAG AGACCCTGCG GCTGGCCAGC TGCGGCCTCA CAGCTGAGAG CTGCAAGGAC 
ATTGCCTCTG GGCTGAGCTC CAGCCAAACC CTGATGCAAT TGGAATTGAG CTTCAACACA 
CTCCTGGACG CAGGAGCTGA GCACCTTTGC CAGGGCCTGA GACAGCCAAC CTGCAAGCTG 
CAGCGACTGT TGCTGGCAGG CTGCGGCCTC ACATCTGGCT GCTGCCAGGA CCTGGCCTCC 
ATGCTCAGGG TCAGCCCCAG CCTGATGGAG CTAAACCTGC TGCAGAATGA CCTGGACAAC 
CTCGGCGTGA GGCTGCTCTG TGAAGGACTC AGGCACCCTT CCTGCCAGCT CATATGCCTA 
TGGCTGGACC AGACTCAGCT GAGCGAGGAG GTGATGGAGA TGCTGAAGGC CCTGGAGAGG 
GAGAAGCCTC AGCTGCTAGT CTCCGGGATA TGGAAGCCAA GTCTGAGGAT CCTTCACGAG 
GGCCCAGGTG GAGCAGAGAT GAGCGATACA TCCTCCCTCA AGCGGCAGAG ACAAGCATCA 
GAGGGCAGCT CTCCTCAAGC AGCACAGGTG AGACTCTTCT GTCCGTCTTC TCCTGCCCCT 
CTGGAGGACA AGCACATGGA GTCTCTGGGG ACCGAGAATG ACCTCTGGGG CCCCACAGGG 
CCTGTGGCTA CCCAGGCAGT TGACGAAGAG AGGAGCCTGT ACCGCGTTCA CTTGCCCGTG 
GCCGGCTTCT ACCACTGGCC CAACACGGGT CTCCGCTTTG AGGTGAGAGG GCCAGTGACC 
GTTGAAATTG AATTCTGTGC CTGGGACCAG TTCCTGAACA GGAATTTCCC ACAGCACAGC 
TGGCTGGCTG CAGGGCCTCT GTTTGACATC AAGGCTGAAC CAGGCGCTGT GGCAGCCGTG 
TTCCTCCCTC ACTTTGTGGC CTTCCGAGGG AACGATGTGG ACATCTCCCA GTTTCAAGTG 
GCCCACTTCA AAGAGGAGGG GATGCTCCTG GAGAAGCCAG CCAGGGTGAC GCCATGTTAC 
GCTGTCCTGG AAAACCCCAG CTTCTCGCCC ATGGGAGTTC TACTGAGGAT GGTCCAAACT 
GCCCTGCGCT TCATTCCCAT CACCTCCACT GTGTTGCTCT ACCATTACCT CCAGCCTGAA 
GAAGTTACCT TCCACCTCTA CCTGATCCCC AGCGATTGCT CCATTCAGAA GGCCATAGAC 
AATGAAGAAA AGAAGTTCCA GTTTGTGCGA ATTCACAAGC CACCCCCGCT GACCCCACTC 
TACATGGGCT CCCGCTACAT TGTGTCTGGT TCAGAGAAGC TGGAAATACT GCCCGAGGAG 
ATCTCAGACG GGCAGTTACT CCAGTTCCTC CGGGCCTCAG AGCCTCACCT GACTCCCCAG 
CTGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006060089.1
CDS	10..3618
Translation	MAIHTKGPHMNKQGACTKTLEETDLNSALYPSFSHSPSAPNLESPSWPTSTKVLTFCKPKQPEPKASLKRILSPLVFASGYRKDLSPSIYQGFPSSPYHESPSQELPNDPTSTAVLRVCEIPRHSCPEPREKEGPGTVWPLVETSGNSCTGSRERYQDQRKETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGPQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELAMSKLTSLEELITKDQSTTVAPAGQVQSQPEQLLFILDGLDELEWVSEEQRAELCLHRSQQQPVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFNVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLRSQTMTALYLRYLAQAIQAQPLGTQLRSFCTLAAEGIWQGKTLFTPGDLKKHGLDWVINSTPLRMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDMGDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAIEMENIFQCKLSWERKRDSLLQWAELELGREHYSLQLFHCLYEIQDEEFLTQAMAHFQGTRVCIRTSMELLVFTFCIRFCSHVRQLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSGNILSRSAVQSLDEALRCPGCQLETLRLASCGLTAESCKDIASGLSSSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDNLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVMEMLKALEREKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPLEDKHMESLGTENDLWGPTGPVATQAVDEERSLYRVHLPVAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQHSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKKFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEEISDGQLLQFLRASEPHLTPQLP

Target ORF information:

RefSeq Version	XM_006060027.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family, pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006060027.1

ORF Insert Sequence:

ATGGCCATCC ACACCAAGGG ACCACACATG AACAAGCAAG GGGCATGCAC CAAGACCCTC 
GAAGAGACAG ACTTGAATTC AGCCCTGTAT CCCTCATTCT CCCACTCGCC AAGTGCACCC 
AACCTGGAAT CTCCCAGCTG GCCCACCTCC ACCAAAGTGT TGACATTCTG CAAGCCTAAG 
CAACCAGAGC CCAAGGCCTC ACTGAAACGG ATTTTGAGCC CCCTAGTTTT CGCATCTGGA 
TATAGGAAAG ACCTTTCCCC AAGCATTTAC CAAGGTTTTC CAAGTTCCCC ATATCATGAG 
TCACCAAGCC AAGAGTTACC CAATGACCCC ACATCCACCG CAGTCCTGAG AGTCTGTGAA 
ATCCCACGTC ATTCCTGTCC AGAGCCCAGA GAGAAGGAGG GTCCTGGGAC TGTATGGCCA 
CTGGTTGAAA CATCTGGAAA CAGCTGCACA GGAAGCAGAG AGAGATACCA GGATCAAAGG 
AAAGAGACAC CTTGCTCCAC CTGGTCATGG AAAAATGAAG ATTTGCACCC AAAATTCATG 
CAGTTGCTAC TTCTACACAC TCCTTACCAC AAAGACTATG AGTCCTTGAA CCGGGAAAGC 
TGGGATCACG GGGTGGTTGA GGGGCAAGGG CATTTGATTG AGGTCAGAGA CTTATTTGGC 
TCAGACCTGG GTCCCCAGGA AGGGCCTCAC ACAGTCATAT TGCATGGAGT TGCTGGAATT 
GGGAAGTCCA CACTGGCCAG GCACATAAGA AGAGCCTGGG AGGAGGGCCA GCTATACAGG 
AACCACTTCC AGCATGTCTT CTACTTCAAC TGCAGAGAGC TGGCCATGTC CAAGCTGACA 
AGTCTGGAGG AACTCATCAC AAAAGACCAG TCCACCACTG TGGCTCCCGC TGGGCAGGTC 
CAGTCTCAGC CGGAGCAGCT GCTCTTCATC CTCGATGGTT TAGATGAACT GGAATGGGTC 
TCAGAGGAGC AGAGGGCAGA GCTCTGTCTG CACCGGAGCC AGCAGCAGCC CGTGCAGACA 
CTGCTGGGCA GTTTGGTGGA GAAAACCATA TTTCCAGAGG CATCCTTGCT GATCACAGCT 
CGGACTGCAG CTCTGCAGAA GTTCATTCCT TCTTTGAAGC AGCCATGTTG GGTGGAGGTC 
CTAGGGTTCT CTGAGTCTTC CAGGAAGGAC TATTTCTATG AATATTTCAC AGAGGAAAGC 
CAAGCAACTA GAGCCTTTAA CGTGGTTGAA TCAAACCAAG CACTCTTGAC CATGTGTCTC 
ACGCCCTTGG TGTCCTGGCT GGCCTGCACT TGCCTGAAGC AGCAGATGGA GGCAGGGGAA 
GAACTCTCAC TGAGGTCACA GACCATGACA GCCCTCTATC TGCGCTACCT TGCCCAAGCT 
ATCCAAGCTC AGCCCCTGGG GACTCAGCTG AGGAGCTTCT GTACTCTTGC TGCTGAGGGT 
ATCTGGCAAG GAAAGACTCT GTTCACTCCA GGGGACCTCA AGAAGCACGG ATTAGATTGG 
GTCATCAACT CCACTCCCTT AAGGATGGAT GTTCTTCAAA AGCACCCCAC GTCTCTGAAC 
TACAGCTTCA TTCACCTGTG TTTCCAGGAG TTCTTTGCAG CAGTGTCCTT TGCCTTAGGG 
GACATGGGGG ACAAAAGTGA TCATCCTAAC AGCACTGGAA GCATGGAAAA GTTGGTAGAA 
GTATATGGGA TCAATAACCT GTTTGGGGCA CCAACCGTGC ATTTCCTATC CGGCCTTTTG 
AGTGAGCAAG GAGCGATAGA GATGGAGAAC ATTTTCCAAT GCAAGCTGTC TTGGGAGAGA 
AAGCGGGATT CACTGCTGCA GTGGGCTGAA TTGGAACTCG GGCGTGAGCA TTACTCTCTG 
CAATTGTTTC ACTGTTTGTA CGAGATTCAG GACGAGGAGT TCCTGACACA AGCGATGGCC 
CATTTCCAAG GAACAAGAGT GTGCATCCGA ACCAGCATGG AGCTGCTCGT GTTCACTTTC 
TGCATTAGAT TCTGCAGCCA CGTGCGGCAG CTTCAACTGA ATGAGGGCAG ACAACATCCA 
GGAGCGTGCA GGCCCACCGA CACAGTGCTG TTGAGCTGGG TCCCGGCCAC AGAGGCCTGC 
TGGCAGGTTC TCTGCTCCGT TCTCCAGGTC ACTGGAAACC TGAAGGAACT GGACCTGAGT 
GGAAACATCT TGAGCCGCTC TGCAGTACAG AGTCTTGATG AGGCCCTGAG ATGCCCTGGG 
TGTCAGCTAG AGACCCTGCG GCTGGCCAGC TGCGGCCTCA CAGCTGAGAG CTGCAAGGAC 
ATTGCCTCTG GGCTGAGCTC CAGCCAAACC CTGATGCAAT TGGAATTGAG CTTCAACACA 
CTCCTGGACG CAGGAGCTGA GCACCTTTGC CAGGGCCTGA GACAGCCAAC CTGCAAGCTG 
CAGCGACTGT TGCTGGCAGG CTGCGGCCTC ACATCTGGCT GCTGCCAGGA CCTGGCCTCC 
ATGCTCAGGG TCAGCCCCAG CCTGATGGAG CTAAACCTGC TGCAGAATGA CCTGGACAAC 
CTCGGCGTGA GGCTGCTCTG TGAAGGACTC AGGCACCCTT CCTGCCAGCT CATATGCCTA 
TGGCTGGACC AGACTCAGCT GAGCGAGGAG GTGATGGAGA TGCTGAAGGC CCTGGAGAGG 
GAGAAGCCTC AGCTGCTAGT CTCCGGGATA TGGAAGCCAA GTCTGAGGAT CCTTCACGAG 
GGCCCAGGTG GAGCAGAGAT GAGCGATACA TCCTCCCTCA AGCGGCAGAG ACAAGCATCA 
GAGGGCAGCT CTCCTCAAGC AGCACAGGTG AGACTCTTCT GTCCGTCTTC TCCTGCCCCT 
CTGGAGGACA AGCACATGGA GTCTCTGGGG ACCGAGAATG ACCTCTGGGG CCCCACAGGG 
CCTGTGGCTA CCCAGGCAGT TGACGAAGAG AGGAGCCTGT ACCGCGTTCA CTTGCCCGTG 
GCCGGCTTCT ACCACTGGCC CAACACGGGT CTCCGCTTTG AGGTGAGAGG GCCAGTGACC 
GTTGAAATTG AATTCTGTGC CTGGGACCAG TTCCTGAACA GGAATTTCCC ACAGCACAGC 
TGGCTGGCTG CAGGGCCTCT GTTTGACATC AAGGCTGAAC CAGGCGCTGT GGCAGCCGTG 
TTCCTCCCTC ACTTTGTGGC CTTCCGAGGG AACGATGTGG ACATCTCCCA GTTTCAAGTG 
GCCCACTTCA AAGAGGAGGG GATGCTCCTG GAGAAGCCAG CCAGGGTGAC GCCATGTTAC 
GCTGTCCTGG AAAACCCCAG CTTCTCGCCC ATGGGAGTTC TACTGAGGAT GGTCCAAACT 
GCCCTGCGCT TCATTCCCAT CACCTCCACT GTGTTGCTCT ACCATTACCT CCAGCCTGAA 
GAAGTTACCT TCCACCTCTA CCTGATCCCC AGCGATTGCT CCATTCAGAA GGCCATAGAC 
AATGAAGAAA AGAAGTTCCA GTTTGTGCGA ATTCACAAGC CACCCCCGCT GACCCCACTC 
TACATGGGCT CCCGCTACAT TGTGTCTGGT TCAGAGAAGC TGGAAATACT GCCCGAGGAG 
ATCTCAGACG GGCAGTTACT CCAGTTCCTC CGGGCCTCAG AGCCTCACCT GACTCCCCAG 
CTGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_006060029.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006060091.1 Bubalus bubalis NLR family, pyrin domain containing 1 (NLRP1), transcript variant X5, mRNA.

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CloneID	OBu16523
Clone ID Related Accession (Same CDS sequence)	XM_006060029.1 , XM_006060028.1
Accession Version	XM_006060029.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784950.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ATGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTCCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAT GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGGCAG 
GTCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGG 
GTCTCAGAGG AGCAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GCCCGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTAGGGT TCTCTGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAACGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGT 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGCCTGA AGCAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAGGTC ACAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGGACTCAG CTGAGGAGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGGC AAGGAAAGAC TCTGTTCACT CCAGGGGACC TCAAGAAGCA CGGATTAGAT 
TGGGTCATCA ACTCCACTCC CTTAAGGATG GATGTTCTTC AAAAGCACCC CACGTCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATGG GGGACAAAAG TGATCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTGTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAT AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG ATTCACTGCT GCAGTGGGCT GAATTGGAAC TCGGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAACAAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGCGG CAGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCGT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAGGCC 
TGCTGGCAGG TTCTCTGCTC CGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTGGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGTCAGC TAGAGACCCT GCGGCTGGCC AGCTGCGGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CTCCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACATCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
AACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGATGG AGATGCTGAA GGCCCTGGAG 
AGGGAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGCGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGACTCT TCTGTCCGTC TTCTCCTGCC 
CCTCTGGAGG ACAAGCACAT GGAGTCTCTG GGGACCGAGA ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCCAGGC AGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
GTGGCCGGCT TCTACCACTG GCCCAACACG GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCAC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCTGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGAAGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGATGG GACTGTGGTG 
TGGGAAGCCT TGGTGAAGCC AGGAGATCTC AGACGGGCAG TTACTCCAGT TCCTCCGGGC 
CTCAGAGCCT CACCTGACTC CCCAGCTGCC CTGAGATTGA GGCACTTTGT GGACCGATAT 
CGGGAGCAGC TGGTGGCTCG AGTGACATCC GTGGACCCTA TCCTGGACAA GTTGCATGGA 
CAGGTGCTGA GCGAAGAACA GTATGAGGGG GTGCGGGCAG AGACCACTGC ACCAGGCCAG 
ATGCGGAAGC TGTTTGGTTT CAGCCGATCC TGGAACTGGG CCTGCAAAGA TCGCCTCTAC 
CAAGCCCTGA AGGAGACCCA TCCTCACCTA ATCATGGAAC TCTGGGAGAA GTGGGGCAGT 
GAAGAGACCA GGCCGGGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006060091.1
CDS	259..3762
Translation	MQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGPQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELAMSKLTSLEELITKDQSTTVAPAGQVQSQPEQLLFILDGLDELEWVSEEQRAELCLHRSQQQPVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFNVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLRSQTMTALYLRYLAQAIQAQPLGTQLRSFCTLAAEGIWQGKTLFTPGDLKKHGLDWVINSTPLRMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDMGDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAIEMENIFQCKLSWERKRDSLLQWAELELGREHYSLQLFHCLYEIQDEEFLTQAMAHFQGTRVCIRTSMELLVFTFCIRFCSHVRQLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSGNILSRSAVQSLDEALRCPGCQLETLRLASCGLTAESCKDIASGLSSSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDNLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVMEMLKALEREKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPLEDKHMESLGTENDLWGPTGPVATQAVDEERSLYRVHLPVAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQHSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKKFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDGTVVWEALVKPGDLRRAVTPVPPGLRASPDSPAALRLRHFVDRYREQLVARVTSVDPILDKLHGQVLSEEQYEGVRAETTAPGQMRKLFGFSRSWNWACKDRLYQALKETHPHLIMELWEKWGSEETRPGS

Target ORF information:

RefSeq Version	XM_006060029.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family, pyrin domain containing 1 (NLRP1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006060029.1

ORF Insert Sequence:

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ATGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTCCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAT GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGGCAG 
GTCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGG 
GTCTCAGAGG AGCAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GCCCGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTAGGGT TCTCTGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAACGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGT 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGCCTGA AGCAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAGGTC ACAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGGACTCAG CTGAGGAGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGGC AAGGAAAGAC TCTGTTCACT CCAGGGGACC TCAAGAAGCA CGGATTAGAT 
TGGGTCATCA ACTCCACTCC CTTAAGGATG GATGTTCTTC AAAAGCACCC CACGTCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATGG GGGACAAAAG TGATCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTGTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAT AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG ATTCACTGCT GCAGTGGGCT GAATTGGAAC TCGGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAACAAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGCGG CAGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCGT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAGGCC 
TGCTGGCAGG TTCTCTGCTC CGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTGGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGTCAGC TAGAGACCCT GCGGCTGGCC AGCTGCGGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CTCCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACATCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
AACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGATGG AGATGCTGAA GGCCCTGGAG 
AGGGAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGCGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGACTCT TCTGTCCGTC TTCTCCTGCC 
CCTCTGGAGG ACAAGCACAT GGAGTCTCTG GGGACCGAGA ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCCAGGC AGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
GTGGCCGGCT TCTACCACTG GCCCAACACG GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCAC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCTGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGAAGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGATGG GACTGTGGTG 
TGGGAAGCCT TGGTGAAGCC AGGAGATCTC AGACGGGCAG TTACTCCAGT TCCTCCGGGC 
CTCAGAGCCT CACCTGACTC CCCAGCTGCC CTGAGATTGA GGCACTTTGT GGACCGATAT 
CGGGAGCAGC TGGTGGCTCG AGTGACATCC GTGGACCCTA TCCTGGACAA GTTGCATGGA 
CAGGTGCTGA GCGAAGAACA GTATGAGGGG GTGCGGGCAG AGACCACTGC ACCAGGCCAG 
ATGCGGAAGC TGTTTGGTTT CAGCCGATCC TGGAACTGGG CCTGCAAAGA TCGCCTCTAC 
CAAGCCCTGA AGGAGACCCA TCCTCACCTA ATCATGGAAC TCTGGGAGAA GTGGGGCAGT 
GAAGAGACCA GGCCGGGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_006060028.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006060090.1 Bubalus bubalis NLR family, pyrin domain containing 1 (NLRP1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu16523
Clone ID Related Accession (Same CDS sequence)	XM_006060029.1 , XM_006060028.1
Accession Version	XM_006060028.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784950.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ATGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTCCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAT GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGGCAG 
GTCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGG 
GTCTCAGAGG AGCAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GCCCGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTAGGGT TCTCTGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAACGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGT 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGCCTGA AGCAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAGGTC ACAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGGACTCAG CTGAGGAGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGGC AAGGAAAGAC TCTGTTCACT CCAGGGGACC TCAAGAAGCA CGGATTAGAT 
TGGGTCATCA ACTCCACTCC CTTAAGGATG GATGTTCTTC AAAAGCACCC CACGTCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATGG GGGACAAAAG TGATCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTGTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAT AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG ATTCACTGCT GCAGTGGGCT GAATTGGAAC TCGGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAACAAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGCGG CAGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCGT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAGGCC 
TGCTGGCAGG TTCTCTGCTC CGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTGGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGTCAGC TAGAGACCCT GCGGCTGGCC AGCTGCGGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CTCCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACATCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
AACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGATGG AGATGCTGAA GGCCCTGGAG 
AGGGAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGCGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGACTCT TCTGTCCGTC TTCTCCTGCC 
CCTCTGGAGG ACAAGCACAT GGAGTCTCTG GGGACCGAGA ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCCAGGC AGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
GTGGCCGGCT TCTACCACTG GCCCAACACG GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCAC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCTGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGAAGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGATGG GACTGTGGTG 
TGGGAAGCCT TGGTGAAGCC AGGAGATCTC AGACGGGCAG TTACTCCAGT TCCTCCGGGC 
CTCAGAGCCT CACCTGACTC CCCAGCTGCC CTGAGATTGA GGCACTTTGT GGACCGATAT 
CGGGAGCAGC TGGTGGCTCG AGTGACATCC GTGGACCCTA TCCTGGACAA GTTGCATGGA 
CAGGTGCTGA GCGAAGAACA GTATGAGGGG GTGCGGGCAG AGACCACTGC ACCAGGCCAG 
ATGCGGAAGC TGTTTGGTTT CAGCCGATCC TGGAACTGGG CCTGCAAAGA TCGCCTCTAC 
CAAGCCCTGA AGGAGACCCA TCCTCACCTA ATCATGGAAC TCTGGGAGAA GTGGGGCAGT 
GAAGAGACCA GGCCGGGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006060090.1
CDS	336..3839
Translation	MQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGPQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELAMSKLTSLEELITKDQSTTVAPAGQVQSQPEQLLFILDGLDELEWVSEEQRAELCLHRSQQQPVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFNVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLRSQTMTALYLRYLAQAIQAQPLGTQLRSFCTLAAEGIWQGKTLFTPGDLKKHGLDWVINSTPLRMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDMGDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAIEMENIFQCKLSWERKRDSLLQWAELELGREHYSLQLFHCLYEIQDEEFLTQAMAHFQGTRVCIRTSMELLVFTFCIRFCSHVRQLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSGNILSRSAVQSLDEALRCPGCQLETLRLASCGLTAESCKDIASGLSSSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDNLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVMEMLKALEREKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPLEDKHMESLGTENDLWGPTGPVATQAVDEERSLYRVHLPVAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQHSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKKFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDGTVVWEALVKPGDLRRAVTPVPPGLRASPDSPAALRLRHFVDRYREQLVARVTSVDPILDKLHGQVLSEEQYEGVRAETTAPGQMRKLFGFSRSWNWACKDRLYQALKETHPHLIMELWEKWGSEETRPGS

Target ORF information:

RefSeq Version	XM_006060028.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family, pyrin domain containing 1 (NLRP1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006060028.1

ORF Insert Sequence:

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ATGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTCCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAT GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGGCAG 
GTCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGG 
GTCTCAGAGG AGCAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GCCCGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTAGGGT TCTCTGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAACGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGT 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGCCTGA AGCAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAGGTC ACAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGGACTCAG CTGAGGAGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGGC AAGGAAAGAC TCTGTTCACT CCAGGGGACC TCAAGAAGCA CGGATTAGAT 
TGGGTCATCA ACTCCACTCC CTTAAGGATG GATGTTCTTC AAAAGCACCC CACGTCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATGG GGGACAAAAG TGATCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTGTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAT AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG ATTCACTGCT GCAGTGGGCT GAATTGGAAC TCGGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAACAAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGCGG CAGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCGT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAGGCC 
TGCTGGCAGG TTCTCTGCTC CGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTGGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGTCAGC TAGAGACCCT GCGGCTGGCC AGCTGCGGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CTCCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACATCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
AACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGATGG AGATGCTGAA GGCCCTGGAG 
AGGGAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGCGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGACTCT TCTGTCCGTC TTCTCCTGCC 
CCTCTGGAGG ACAAGCACAT GGAGTCTCTG GGGACCGAGA ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCCAGGC AGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
GTGGCCGGCT TCTACCACTG GCCCAACACG GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCAC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCTGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGAAGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGATGG GACTGTGGTG 
TGGGAAGCCT TGGTGAAGCC AGGAGATCTC AGACGGGCAG TTACTCCAGT TCCTCCGGGC 
CTCAGAGCCT CACCTGACTC CCCAGCTGCC CTGAGATTGA GGCACTTTGT GGACCGATAT 
CGGGAGCAGC TGGTGGCTCG AGTGACATCC GTGGACCCTA TCCTGGACAA GTTGCATGGA 
CAGGTGCTGA GCGAAGAACA GTATGAGGGG GTGCGGGCAG AGACCACTGC ACCAGGCCAG 
ATGCGGAAGC TGTTTGGTTT CAGCCGATCC TGGAACTGGG CCTGCAAAGA TCGCCTCTAC 
CAAGCCCTGA AGGAGACCCA TCCTCACCTA ATCATGGAAC TCTGGGAGAA GTGGGGCAGT 
GAAGAGACCA GGCCGGGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_025280774.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025136559.1 Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.

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CloneID	OBu62101
Clone ID Related Accession (Same CDS sequence)	XM_025280774.1
Accession Version	XM_025280774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGCTC TGACAAGGTC TCCCAGCCCA GCAGCGATGG CCGGTGAAGC CCGGAGGCTG 
GAGGACTTGG AGGAAAAGAA GAAAGAGAAG CTGAAGGAGC TCCAGCTTTG GCTGCCTGAT 
GAGAAGCCCT GCTTGCAGTC TCCTAAGGTG GTCTATGCTC AACCAGAGAA GCTAAGGATC 
CGAAGGATCA TGATGATCCC CTCAGTGATC AAGGAGGTAC AGCACGGGTG CATGGCCATC 
CACACCAAGG GACCACACAT GAACAAGCAA GGGGCATGCA CCAAGACCCT CGAAGAGACA 
GACTTGAATT CAGCCCTGTA TCCCTCATTC TCCCACTCGC CAAGTGCACC CAACCTGGAA 
TCTCCCAGCT GGCCCACCTC CACCAAAGTG TTGACATTCT GCAAGCCTAA GCAACCAGAG 
CCCAAGGCCT CACTGAAACG GATTTTGAGC CCCCTAGTTT TCGCATCTGG ATATAGGAAA 
GACCTTTCCC CAAGCATTTA CCAAGGTTTT CCAAGTTCCC CATATCATGA GTCACCAAGC 
CAAGAGTTAC CCAATGACCC CACATCCACC GCAGTCCTGA GAGTCTGTGA AATCCCACGT 
CATTCCTGTC CAGAGCCCAG AGAGAAGGAG GGTCCTGGGA CTGTATGGCC ACTGGTTGAA 
ACATCTGGAA ACAGCTGCAC AGGAAGCAGA GAGAGATACC AGGATCAAAG GAAAGAGACA 
CCTTGCTCCA CCTGGTCATG GAAAAATGAA GATTTGCACC CAAAATTCAT GCAGTTGCTA 
CTTCTACACA CTCCTTACCA CAAAGACTAT GAGTCCTTGA ACCGGGAAAG CTGGGATCAC 
GGGGTGGTTG AGGGGCAAGG GCATTTGATT GAGGTCAGAG ACTTATTTGG CTCAGACCTG 
GGTCCCCAGG AAGGGCCTCA CACAGTCATA TTGCATGGAG TTGCTGGAAT TGGGAAGTCC 
ACACTGGCCA GGCACATAAG AAGAGCCTGG GAGGAGGGCC AGCTATACAG GAACCACTTC 
CAGCATGTCT TCTACTTCAA CTGCAGAGAG CTGGCCATGT CCAAGCTGAC AAGTCTGGAG 
GAACTCATCA CAAAAGACCA GTCCACCACT GTGGCTCCCG CTGGGCAGGT CCAGTCTCAG 
CCGGAGCAGC TGCTCTTCAT CCTCGATGGT TTAGATGAAC TGGAATGGGT CTCAGAGGAG 
CAGAGGGCAG AGCTCTGTCT GCACCGGAGC CAGCAGCAGC CCGTGCAGAC ACTGCTGGGC 
AGTTTGGTGG AGAAAACCAT ATTTCCAGAG GCATCCTTGC TGATCACAGC TCGGACTGCA 
GCTCTGCAGA AGTTCATTCC TTCTTTGAAG CAGCCATGTT GGGTGGAGGT CCTAGGGTTC 
TCTGAGTCTT CCAGGAAGGA CTATTTCTAT GAATATTTCA CAGAGGAAAG CCAAGCAACT 
AGAGCCTTTA ACGTGGTTGA ATCAAACCAA GCACTCTTGA CCATGTGTCT CACGCCCTTG 
GTGTCCTGGC TGGCCTGCAC TTGTCTGAAG CAGCAGATGG AGGCAGGGGA AGAACTCTCA 
CTGAGGTCAC AGACCATGAC AGCCCTCTAT CTGCGCTACC TTGCCCAAGC TATCCAAGCT 
CAGCCCCTGG GGACTCAGCT GAGGAGCTTC TGTACTCTTG CTGCTGAGGG TATCTGGCAA 
GGAAAGACTC TGTTCACTCC AGGGGACCTC AAGAAGCACG GATTAGATTG GGTCATCAAC 
TCCACTCCCT TAAGGATGGA TGTTCTTCAA AAGCACCCCA CGTCTCTGAA CTACAGCTTC 
ATTCACCTGT GTTTCCAGGA GTTCTTTGCA GCAGTGTCCT TTGCCTTAGG GGACATGGGG 
GACAAAAGTG ACCATCCTAA CAGCACTGGA AGCATGGAAA AGTTGGTAGA AGTATATGGG 
ATCAATAACC TGTTTGGGGC ACCAACCGTG CATTTCCTAT CCGGCCTTTT GAGTGAGCAA 
GGAGCGATAG AGATGGAGAA CATTTTCCAA TGCAAGCTGT CTTGGGAGAG AAAGCGGGAT 
TCACTGCTGC AGTGGGCTGA ATTGGAACTC GGGCGTGAGC ATTACTCTCT GCAATTGTTT 
CACTGTTTGT ACGAGATTCA GGACGAGGAG TTCCTGACAC AAGCGATGGC CCATTTCCAA 
GGAACAAGAG TGTGCATCCG AACCAGCATG GAGCTGCTCG TGTTCACTTT CTGCATTAGA 
TTCTGCAGCC ACGTGCGGCA GCTTCAACTG AATGAGGGCA GACAACATCC AGGAGCGTGC 
AGGCCCACCG ACACAGTGCT GTTGAGCTGG GTCCCGGCCA CAGAGGCCTG CTGGCAGGTT 
CTCTGCTCCG TTCTCCAGGT CACTGGAAAC CTGAAGGAAC TGGACCTGAG TGGAAACATC 
TTGAGCCGCT CTGCAGTACA GAGTCTTGAT GAGGCCCTGA GATGCCCTGG GTGTCAGCTA 
GAGACCCTGC GGCTGGCCAG CTGCGGCCTC ACAGCTGAGA GCTGCAAGGA CATTGCCTCT 
GGGCTGAGCT CCAGCCAAAC CCTGATGCAA TTGGAATTGA GCTTCAACAC ACTCCTGGAC 
GCAGGAGCTG AGCACCTTTG CCAGGGCCTG AGACAGCCAA CCTGCAAGCT GCAGCGACTG 
TTGCTGGCAG GCTGCGGCCT CACGTCTGGC TGCTGCCAGG ACCTGGCCTC CATGCTCAGG 
GTCAGCCCCA GCCTGATGGA GCTAAACCTG CTGCAGAATG ACCTGGACAA CCTCGGCGTG 
AGGCTGCTCT GTGAAGGACT CAGGCACCCT TCCTGCCAGC TCATATGCCT ATGGCTGGAC 
CAGACTCAGC TGAGCGAGGA GGTGATGGAG ATGCTGAAGG CCCTGGAGAG GGAGAAGCCT 
CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA AGTCTGAGGA TCCTTCACGA GGGCCCAGGT 
GGAGCAGAGA TGAGCGATAC ATCCTCCCTC AAGCGGCAGA GACAAGCATC AGAGGGCAGC 
TCTCCTCAAG CAGCACAGGT GAGACTCTTC TGTCCGTCTT CTCCTGCCCC TCTGGAGGAC 
AAGCACATGG AGTCTCTGGG GACCGAGAAT GACCTCTGGG GCCCCACAGG GCCTGTGGCT 
ACCCAGGCAG TTGACGAAGA GAGGAGCCTG TACCGCGTTC ACTTGCCCGT GGCCGGCTTC 
TACCACTGGC CCAACACGGG TCTCCGCTTT GAGGTGAGAG GGCCAGTGAC CGTTGAAATT 
GAATTCTGTG CCTGGGACCA GTTCCTGAAC AGGAATTTCC CACAGCACAG CTGGCTGGCT 
GCAGGGCCTC TGTTTGACAT CAAGGCTGAA CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT 
CACTTTGTGG CCTTCCGAGG GAACGATGTG GACATCTCCC AGTTTCAAGT GGCCCACTTC 
AAAGAGGAGG GGATGCTCCT GGAGAAGCCA GCCAGGGTGA CGCCATGTTA CGCTGTCCTG 
GAAAACCCCA GCTTCTCGCC CATGGGAGTT CTACTGAGGA TGGTCCAAAC TGCCCTGCGC 
TTCATTCCCA TCACCTCCAC TGTGTTGCTC TACCATTACC TCCAGCCTGA AGAAGTTACC 
TTCCACCTCT ACCTGATCCC CAGCGATTGC TCCATTCAGA AGGCCATAGA CAATGAAGAA 
AAGAAGTTCC AGTTTGTGCG AATTCACAAG CCACCCCCGC TGACCCCACT CTACATGGGC 
TCCCGCTACA TTGTGTCTGG TTCAGAGAAG CTGGAAATAC TGCCCGAGGA ATTGGAGCTC 
TGCTATCGGA GCCCCAGAGA ATCTCAGCTG TTCTCTGAGT TCTACGTTCG CCATTTGAGG 
TCAGGGATCA GGTTGCAAAT AAGAAGCAAG AAAGACTGGG ACTGTGGTGT GGGAAGCCTT 
GGTGAAGCCA GGAGATCTCA GACGGGCAGT TACTCCAGTT CCTCCAGGCC TCAGAGCCTC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025136559.1
CDS	244..4209
Translation	MPALTRSPSPAAMAGEARRLEDLEEKKKEKLKELQLWLPDEKPCLQSPKVVYAQPEKLRIRRIMMIPSVIKEVQHGCMAIHTKGPHMNKQGACTKTLEETDLNSALYPSFSHSPSAPNLESPSWPTSTKVLTFCKPKQPEPKASLKRILSPLVFASGYRKDLSPSIYQGFPSSPYHESPSQELPNDPTSTAVLRVCEIPRHSCPEPREKEGPGTVWPLVETSGNSCTGSRERYQDQRKETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGPQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELAMSKLTSLEELITKDQSTTVAPAGQVQSQPEQLLFILDGLDELEWVSEEQRAELCLHRSQQQPVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFNVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLRSQTMTALYLRYLAQAIQAQPLGTQLRSFCTLAAEGIWQGKTLFTPGDLKKHGLDWVINSTPLRMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDMGDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAIEMENIFQCKLSWERKRDSLLQWAELELGREHYSLQLFHCLYEIQDEEFLTQAMAHFQGTRVCIRTSMELLVFTFCIRFCSHVRQLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSGNILSRSAVQSLDEALRCPGCQLETLRLASCGLTAESCKDIASGLSSSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDNLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVMEMLKALEREKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPLEDKHMESLGTENDLWGPTGPVATQAVDEERSLYRVHLPVAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQHSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKKFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDWDCGVGSLGEARRSQTGSYSSSSRPQSLT

Target ORF information:

RefSeq Version	XM_025280774.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025280774.1

ORF Insert Sequence:

ATGCCTGCTC TGACAAGGTC TCCCAGCCCA GCAGCGATGG CCGGTGAAGC CCGGAGGCTG 
GAGGACTTGG AGGAAAAGAA GAAAGAGAAG CTGAAGGAGC TCCAGCTTTG GCTGCCTGAT 
GAGAAGCCCT GCTTGCAGTC TCCTAAGGTG GTCTATGCTC AACCAGAGAA GCTAAGGATC 
CGAAGGATCA TGATGATCCC CTCAGTGATC AAGGAGGTAC AGCACGGGTG CATGGCCATC 
CACACCAAGG GACCACACAT GAACAAGCAA GGGGCATGCA CCAAGACCCT CGAAGAGACA 
GACTTGAATT CAGCCCTGTA TCCCTCATTC TCCCACTCGC CAAGTGCACC CAACCTGGAA 
TCTCCCAGCT GGCCCACCTC CACCAAAGTG TTGACATTCT GCAAGCCTAA GCAACCAGAG 
CCCAAGGCCT CACTGAAACG GATTTTGAGC CCCCTAGTTT TCGCATCTGG ATATAGGAAA 
GACCTTTCCC CAAGCATTTA CCAAGGTTTT CCAAGTTCCC CATATCATGA GTCACCAAGC 
CAAGAGTTAC CCAATGACCC CACATCCACC GCAGTCCTGA GAGTCTGTGA AATCCCACGT 
CATTCCTGTC CAGAGCCCAG AGAGAAGGAG GGTCCTGGGA CTGTATGGCC ACTGGTTGAA 
ACATCTGGAA ACAGCTGCAC AGGAAGCAGA GAGAGATACC AGGATCAAAG GAAAGAGACA 
CCTTGCTCCA CCTGGTCATG GAAAAATGAA GATTTGCACC CAAAATTCAT GCAGTTGCTA 
CTTCTACACA CTCCTTACCA CAAAGACTAT GAGTCCTTGA ACCGGGAAAG CTGGGATCAC 
GGGGTGGTTG AGGGGCAAGG GCATTTGATT GAGGTCAGAG ACTTATTTGG CTCAGACCTG 
GGTCCCCAGG AAGGGCCTCA CACAGTCATA TTGCATGGAG TTGCTGGAAT TGGGAAGTCC 
ACACTGGCCA GGCACATAAG AAGAGCCTGG GAGGAGGGCC AGCTATACAG GAACCACTTC 
CAGCATGTCT TCTACTTCAA CTGCAGAGAG CTGGCCATGT CCAAGCTGAC AAGTCTGGAG 
GAACTCATCA CAAAAGACCA GTCCACCACT GTGGCTCCCG CTGGGCAGGT CCAGTCTCAG 
CCGGAGCAGC TGCTCTTCAT CCTCGATGGT TTAGATGAAC TGGAATGGGT CTCAGAGGAG 
CAGAGGGCAG AGCTCTGTCT GCACCGGAGC CAGCAGCAGC CCGTGCAGAC ACTGCTGGGC 
AGTTTGGTGG AGAAAACCAT ATTTCCAGAG GCATCCTTGC TGATCACAGC TCGGACTGCA 
GCTCTGCAGA AGTTCATTCC TTCTTTGAAG CAGCCATGTT GGGTGGAGGT CCTAGGGTTC 
TCTGAGTCTT CCAGGAAGGA CTATTTCTAT GAATATTTCA CAGAGGAAAG CCAAGCAACT 
AGAGCCTTTA ACGTGGTTGA ATCAAACCAA GCACTCTTGA CCATGTGTCT CACGCCCTTG 
GTGTCCTGGC TGGCCTGCAC TTGTCTGAAG CAGCAGATGG AGGCAGGGGA AGAACTCTCA 
CTGAGGTCAC AGACCATGAC AGCCCTCTAT CTGCGCTACC TTGCCCAAGC TATCCAAGCT 
CAGCCCCTGG GGACTCAGCT GAGGAGCTTC TGTACTCTTG CTGCTGAGGG TATCTGGCAA 
GGAAAGACTC TGTTCACTCC AGGGGACCTC AAGAAGCACG GATTAGATTG GGTCATCAAC 
TCCACTCCCT TAAGGATGGA TGTTCTTCAA AAGCACCCCA CGTCTCTGAA CTACAGCTTC 
ATTCACCTGT GTTTCCAGGA GTTCTTTGCA GCAGTGTCCT TTGCCTTAGG GGACATGGGG 
GACAAAAGTG ACCATCCTAA CAGCACTGGA AGCATGGAAA AGTTGGTAGA AGTATATGGG 
ATCAATAACC TGTTTGGGGC ACCAACCGTG CATTTCCTAT CCGGCCTTTT GAGTGAGCAA 
GGAGCGATAG AGATGGAGAA CATTTTCCAA TGCAAGCTGT CTTGGGAGAG AAAGCGGGAT 
TCACTGCTGC AGTGGGCTGA ATTGGAACTC GGGCGTGAGC ATTACTCTCT GCAATTGTTT 
CACTGTTTGT ACGAGATTCA GGACGAGGAG TTCCTGACAC AAGCGATGGC CCATTTCCAA 
GGAACAAGAG TGTGCATCCG AACCAGCATG GAGCTGCTCG TGTTCACTTT CTGCATTAGA 
TTCTGCAGCC ACGTGCGGCA GCTTCAACTG AATGAGGGCA GACAACATCC AGGAGCGTGC 
AGGCCCACCG ACACAGTGCT GTTGAGCTGG GTCCCGGCCA CAGAGGCCTG CTGGCAGGTT 
CTCTGCTCCG TTCTCCAGGT CACTGGAAAC CTGAAGGAAC TGGACCTGAG TGGAAACATC 
TTGAGCCGCT CTGCAGTACA GAGTCTTGAT GAGGCCCTGA GATGCCCTGG GTGTCAGCTA 
GAGACCCTGC GGCTGGCCAG CTGCGGCCTC ACAGCTGAGA GCTGCAAGGA CATTGCCTCT 
GGGCTGAGCT CCAGCCAAAC CCTGATGCAA TTGGAATTGA GCTTCAACAC ACTCCTGGAC 
GCAGGAGCTG AGCACCTTTG CCAGGGCCTG AGACAGCCAA CCTGCAAGCT GCAGCGACTG 
TTGCTGGCAG GCTGCGGCCT CACGTCTGGC TGCTGCCAGG ACCTGGCCTC CATGCTCAGG 
GTCAGCCCCA GCCTGATGGA GCTAAACCTG CTGCAGAATG ACCTGGACAA CCTCGGCGTG 
AGGCTGCTCT GTGAAGGACT CAGGCACCCT TCCTGCCAGC TCATATGCCT ATGGCTGGAC 
CAGACTCAGC TGAGCGAGGA GGTGATGGAG ATGCTGAAGG CCCTGGAGAG GGAGAAGCCT 
CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA AGTCTGAGGA TCCTTCACGA GGGCCCAGGT 
GGAGCAGAGA TGAGCGATAC ATCCTCCCTC AAGCGGCAGA GACAAGCATC AGAGGGCAGC 
TCTCCTCAAG CAGCACAGGT GAGACTCTTC TGTCCGTCTT CTCCTGCCCC TCTGGAGGAC 
AAGCACATGG AGTCTCTGGG GACCGAGAAT GACCTCTGGG GCCCCACAGG GCCTGTGGCT 
ACCCAGGCAG TTGACGAAGA GAGGAGCCTG TACCGCGTTC ACTTGCCCGT GGCCGGCTTC 
TACCACTGGC CCAACACGGG TCTCCGCTTT GAGGTGAGAG GGCCAGTGAC CGTTGAAATT 
GAATTCTGTG CCTGGGACCA GTTCCTGAAC AGGAATTTCC CACAGCACAG CTGGCTGGCT 
GCAGGGCCTC TGTTTGACAT CAAGGCTGAA CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT 
CACTTTGTGG CCTTCCGAGG GAACGATGTG GACATCTCCC AGTTTCAAGT GGCCCACTTC 
AAAGAGGAGG GGATGCTCCT GGAGAAGCCA GCCAGGGTGA CGCCATGTTA CGCTGTCCTG 
GAAAACCCCA GCTTCTCGCC CATGGGAGTT CTACTGAGGA TGGTCCAAAC TGCCCTGCGC 
TTCATTCCCA TCACCTCCAC TGTGTTGCTC TACCATTACC TCCAGCCTGA AGAAGTTACC 
TTCCACCTCT ACCTGATCCC CAGCGATTGC TCCATTCAGA AGGCCATAGA CAATGAAGAA 
AAGAAGTTCC AGTTTGTGCG AATTCACAAG CCACCCCCGC TGACCCCACT CTACATGGGC 
TCCCGCTACA TTGTGTCTGG TTCAGAGAAG CTGGAAATAC TGCCCGAGGA ATTGGAGCTC 
TGCTATCGGA GCCCCAGAGA ATCTCAGCTG TTCTCTGAGT TCTACGTTCG CCATTTGAGG 
TCAGGGATCA GGTTGCAAAT AAGAAGCAAG AAAGACTGGG ACTGTGGTGT GGGAAGCCTT 
GGTGAAGCCA GGAGATCTCA GACGGGCAGT TACTCCAGTT CCTCCAGGCC TCAGAGCCTC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_006060025.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006060087.2 Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu62102
Clone ID Related Accession (Same CDS sequence)	XM_006060025.2
Accession Version	XM_006060025.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006060025.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGTG AAGCCCGGAG GCTGGAGGAC TTGGAGGAAA AGAAGAAAGA GAAGCTGAAG 
GAGCTCCAGC TTTGGCTGCC TGATGAGAAG CCCTGCTTGC AGTCTCCTAA GGTGGTCTAT 
GCTCAACCAG AGAAGCTAAG GATCCGAAGG ATCATGATGA TCCCCTCAGT GATCAAGGAG 
GTACAGCACG GGTGCATGGC CATCCACACC AAGGGACCAC ACATGAACAA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATCCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAATCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGAGCCCAAG GCCTCACTGA AACGGATTTT GAGCCCCCTA 
GTTTTCGCAT CTGGATATAG GAAAGACCTT TCCCCAAGCA TTTACCAAGG TTTTCCAAGT 
TCCCCATATC ATGAGTCACC AAGCCAAGAG TTACCCAATG ACCCCACATC CACCGCAGTC 
CTGAGAGTCT GTGAAATCCC ACGTCATTCC TGTCCAGAGC CCAGAGAGAA GGAGGGTCCT 
GGGACTGTAT GGCCACTGGT TGAAACATCT GGAAACAGCT GCACAGGAAG CAGAGAGAGA 
TACCAGGATC AAAGGAAAGA GACACCTTGC TCCACCTGGT CATGGAAAAA TGAAGATTTG 
CACCCAAAAT TCATGCAGTT GCTACTTCTA CACACTCCTT ACCACAAAGA CTATGAGTCC 
TTGAACCGGG AAAGCTGGGA TCACGGGGTG GTTGAGGGGC AAGGGCATTT GATTGAGGTC 
AGAGACTTAT TTGGCTCAGA CCTGGGTCCC CAGGAAGGGC CTCACACAGT CATATTGCAT 
GGAGTTGCTG GAATTGGGAA GTCCACACTG GCCAGGCACA TAAGAAGAGC CTGGGAGGAG 
GGCCAGCTAT ACAGGAACCA CTTCCAGCAT GTCTTCTACT TCAACTGCAG AGAGCTGGCC 
ATGTCCAAGC TGACAAGTCT GGAGGAACTC ATCACAAAAG ACCAGTCCAC CACTGTGGCT 
CCCGCTGGGC AGGTCCAGTC TCAGCCGGAG CAGCTGCTCT TCATCCTCGA TGGTTTAGAT 
GAACTGGAAT GGGTCTCAGA GGAGCAGAGG GCAGAGCTCT GTCTGCACCG GAGCCAGCAG 
CAGCCCGTGC AGACACTGCT GGGCAGTTTG GTGGAGAAAA CCATATTTCC AGAGGCATCC 
TTGCTGATCA CAGCTCGGAC TGCAGCTCTG CAGAAGTTCA TTCCTTCTTT GAAGCAGCCA 
TGTTGGGTGG AGGTCCTAGG GTTCTCTGAG TCTTCCAGGA AGGACTATTT CTATGAATAT 
TTCACAGAGG AAAGCCAAGC AACTAGAGCC TTTAACGTGG TTGAATCAAA CCAAGCACTC 
TTGACCATGT GTCTCACGCC CTTGGTGTCC TGGCTGGCCT GCACTTGTCT GAAGCAGCAG 
ATGGAGGCAG GGGAAGAACT CTCACTGAGG TCACAGACCA TGACAGCCCT CTATCTGCGC 
TACCTTGCCC AAGCTATCCA AGCTCAGCCC CTGGGGACTC AGCTGAGGAG CTTCTGTACT 
CTTGCTGCTG AGGGTATCTG GCAAGGAAAG ACTCTGTTCA CTCCAGGGGA CCTCAAGAAG 
CACGGATTAG ATTGGGTCAT CAACTCCACT CCCTTAAGGA TGGATGTTCT TCAAAAGCAC 
CCCACGTCTC TGAACTACAG CTTCATTCAC CTGTGTTTCC AGGAGTTCTT TGCAGCAGTG 
TCCTTTGCCT TAGGGGACAT GGGGGACAAA AGTGACCATC CTAACAGCAC TGGAAGCATG 
GAAAAGTTGG TAGAAGTATA TGGGATCAAT AACCTGTTTG GGGCACCAAC CGTGCATTTC 
CTATCCGGCC TTTTGAGTGA GCAAGGAGCG ATAGAGATGG AGAACATTTT CCAATGCAAG 
CTGTCTTGGG AGAGAAAGCG GGATTCACTG CTGCAGTGGG CTGAATTGGA ACTCGGGCGT 
GAGCATTACT CTCTGCAATT GTTTCACTGT TTGTACGAGA TTCAGGACGA GGAGTTCCTG 
ACACAAGCGA TGGCCCATTT CCAAGGAACA AGAGTGTGCA TCCGAACCAG CATGGAGCTG 
CTCGTGTTCA CTTTCTGCAT TAGATTCTGC AGCCACGTGC GGCAGCTTCA ACTGAATGAG 
GGCAGACAAC ATCCAGGAGC GTGCAGGCCC ACCGACACAG TGCTGTTGAG CTGGGTCCCG 
GCCACAGAGG CCTGCTGGCA GGTTCTCTGC TCCGTTCTCC AGGTCACTGG AAACCTGAAG 
GAACTGGACC TGAGTGGAAA CATCTTGAGC CGCTCTGCAG TACAGAGTCT TGATGAGGCC 
CTGAGATGCC CTGGGTGTCA GCTAGAGACC CTGCGGCTGG CCAGCTGCGG CCTCACAGCT 
GAGAGCTGCA AGGACATTGC CTCTGGGCTG AGCTCCAGCC AAACCCTGAT GCAATTGGAA 
TTGAGCTTCA ACACACTCCT GGACGCAGGA GCTGAGCACC TTTGCCAGGG CCTGAGACAG 
CCAACCTGCA AGCTGCAGCG ACTGTTGCTG GCAGGCTGCG GCCTCACGTC TGGCTGCTGC 
CAGGACCTGG CCTCCATGCT CAGGGTCAGC CCCAGCCTGA TGGAGCTAAA CCTGCTGCAG 
AATGACCTGG ACAACCTCGG CGTGAGGCTG CTCTGTGAAG GACTCAGGCA CCCTTCCTGC 
CAGCTCATAT GCCTATGGCT GGACCAGACT CAGCTGAGCG AGGAGGTGAT GGAGATGCTG 
AAGGCCCTGG AGAGGGAGAA GCCTCAGCTG CTAGTCTCCG GGATATGGAA GCCAAGTCTG 
AGGATCCTTC ACGAGGGCCC AGGTGGAGCA GAGATGAGCG ATACATCCTC CCTCAAGCGG 
CAGAGACAAG CATCAGAGGG CAGCTCTCCT CAAGCAGCAC AGGTGAGACT CTTCTGTCCG 
TCTTCTCCTG CCCCTCTGGA GGACAAGCAC ATGGAGTCTC TGGGGACCGA GAATGACCTC 
TGGGGCCCCA CAGGGCCTGT GGCTACCCAG GCAGTTGACG AAGAGAGGAG CCTGTACCGC 
GTTCACTTGC CCGTGGCCGG CTTCTACCAC TGGCCCAACA CGGGTCTCCG CTTTGAGGTG 
AGAGGGCCAG TGACCGTTGA AATTGAATTC TGTGCCTGGG ACCAGTTCCT GAACAGGAAT 
TTCCCACAGC ACAGCTGGCT GGCTGCAGGG CCTCTGTTTG ACATCAAGGC TGAACCAGGC 
GCTGTGGCAG CCGTGTTCCT CCCTCACTTT GTGGCCTTCC GAGGGAACGA TGTGGACATC 
TCCCAGTTTC AAGTGGCCCA CTTCAAAGAG GAGGGGATGC TCCTGGAGAA GCCAGCCAGG 
GTGACGCCAT GTTACGCTGT CCTGGAAAAC CCCAGCTTCT CGCCCATGGG AGTTCTACTG 
AGGATGGTCC AAACTGCCCT GCGCTTCATT CCCATCACCT CCACTGTGTT GCTCTACCAT 
TACCTCCAGC CTGAAGAAGT TACCTTCCAC CTCTACCTGA TCCCCAGCGA TTGCTCCATT 
CAGAAGGCCA TAGACAATGA AGAAAAGAAG TTCCAGTTTG TGCGAATTCA CAAGCCACCC 
CCGCTGACCC CACTCTACAT GGGCTCCCGC TACATTGTGT CTGGTTCAGA GAAGCTGGAA 
ATACTGCCCG AGGAATTGGA GCTCTGCTAT CGGAGCCCCA GAGAATCTCA GCTGTTCTCT 
GAGTTCTACG TTCGCCATTT GAGGTCAGGG ATCAGGTTGC AAATAAGAAG CAAGAAAGAC 
TGGGACTGTG GTGTGGGAAG CCTTGGTGAA GCCAGGAGAT CTCAGACGGG CAGTTACTCC 
AGTTCCTCCA GGCCTCAGAG CCTCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006060087.2
CDS	458..4387
Translation	MAGEARRLEDLEEKKKEKLKELQLWLPDEKPCLQSPKVVYAQPEKLRIRRIMMIPSVIKEVQHGCMAIHTKGPHMNKQGACTKTLEETDLNSALYPSFSHSPSAPNLESPSWPTSTKVLTFCKPKQPEPKASLKRILSPLVFASGYRKDLSPSIYQGFPSSPYHESPSQELPNDPTSTAVLRVCEIPRHSCPEPREKEGPGTVWPLVETSGNSCTGSRERYQDQRKETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGPQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELAMSKLTSLEELITKDQSTTVAPAGQVQSQPEQLLFILDGLDELEWVSEEQRAELCLHRSQQQPVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFNVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLRSQTMTALYLRYLAQAIQAQPLGTQLRSFCTLAAEGIWQGKTLFTPGDLKKHGLDWVINSTPLRMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDMGDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAIEMENIFQCKLSWERKRDSLLQWAELELGREHYSLQLFHCLYEIQDEEFLTQAMAHFQGTRVCIRTSMELLVFTFCIRFCSHVRQLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSGNILSRSAVQSLDEALRCPGCQLETLRLASCGLTAESCKDIASGLSSSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDNLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVMEMLKALEREKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPLEDKHMESLGTENDLWGPTGPVATQAVDEERSLYRVHLPVAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQHSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKKFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDWDCGVGSLGEARRSQTGSYSSSSRPQSLT

Target ORF information:

RefSeq Version	XM_006060025.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006060025.2

ORF Insert Sequence:

ATGGCCGGTG AAGCCCGGAG GCTGGAGGAC TTGGAGGAAA AGAAGAAAGA GAAGCTGAAG 
GAGCTCCAGC TTTGGCTGCC TGATGAGAAG CCCTGCTTGC AGTCTCCTAA GGTGGTCTAT 
GCTCAACCAG AGAAGCTAAG GATCCGAAGG ATCATGATGA TCCCCTCAGT GATCAAGGAG 
GTACAGCACG GGTGCATGGC CATCCACACC AAGGGACCAC ACATGAACAA GCAAGGGGCA 
TGCACCAAGA CCCTCGAAGA GACAGACTTG AATTCAGCCC TGTATCCCTC ATTCTCCCAC 
TCGCCAAGTG CACCCAACCT GGAATCTCCC AGCTGGCCCA CCTCCACCAA AGTGTTGACA 
TTCTGCAAGC CTAAGCAACC AGAGCCCAAG GCCTCACTGA AACGGATTTT GAGCCCCCTA 
GTTTTCGCAT CTGGATATAG GAAAGACCTT TCCCCAAGCA TTTACCAAGG TTTTCCAAGT 
TCCCCATATC ATGAGTCACC AAGCCAAGAG TTACCCAATG ACCCCACATC CACCGCAGTC 
CTGAGAGTCT GTGAAATCCC ACGTCATTCC TGTCCAGAGC CCAGAGAGAA GGAGGGTCCT 
GGGACTGTAT GGCCACTGGT TGAAACATCT GGAAACAGCT GCACAGGAAG CAGAGAGAGA 
TACCAGGATC AAAGGAAAGA GACACCTTGC TCCACCTGGT CATGGAAAAA TGAAGATTTG 
CACCCAAAAT TCATGCAGTT GCTACTTCTA CACACTCCTT ACCACAAAGA CTATGAGTCC 
TTGAACCGGG AAAGCTGGGA TCACGGGGTG GTTGAGGGGC AAGGGCATTT GATTGAGGTC 
AGAGACTTAT TTGGCTCAGA CCTGGGTCCC CAGGAAGGGC CTCACACAGT CATATTGCAT 
GGAGTTGCTG GAATTGGGAA GTCCACACTG GCCAGGCACA TAAGAAGAGC CTGGGAGGAG 
GGCCAGCTAT ACAGGAACCA CTTCCAGCAT GTCTTCTACT TCAACTGCAG AGAGCTGGCC 
ATGTCCAAGC TGACAAGTCT GGAGGAACTC ATCACAAAAG ACCAGTCCAC CACTGTGGCT 
CCCGCTGGGC AGGTCCAGTC TCAGCCGGAG CAGCTGCTCT TCATCCTCGA TGGTTTAGAT 
GAACTGGAAT GGGTCTCAGA GGAGCAGAGG GCAGAGCTCT GTCTGCACCG GAGCCAGCAG 
CAGCCCGTGC AGACACTGCT GGGCAGTTTG GTGGAGAAAA CCATATTTCC AGAGGCATCC 
TTGCTGATCA CAGCTCGGAC TGCAGCTCTG CAGAAGTTCA TTCCTTCTTT GAAGCAGCCA 
TGTTGGGTGG AGGTCCTAGG GTTCTCTGAG TCTTCCAGGA AGGACTATTT CTATGAATAT 
TTCACAGAGG AAAGCCAAGC AACTAGAGCC TTTAACGTGG TTGAATCAAA CCAAGCACTC 
TTGACCATGT GTCTCACGCC CTTGGTGTCC TGGCTGGCCT GCACTTGTCT GAAGCAGCAG 
ATGGAGGCAG GGGAAGAACT CTCACTGAGG TCACAGACCA TGACAGCCCT CTATCTGCGC 
TACCTTGCCC AAGCTATCCA AGCTCAGCCC CTGGGGACTC AGCTGAGGAG CTTCTGTACT 
CTTGCTGCTG AGGGTATCTG GCAAGGAAAG ACTCTGTTCA CTCCAGGGGA CCTCAAGAAG 
CACGGATTAG ATTGGGTCAT CAACTCCACT CCCTTAAGGA TGGATGTTCT TCAAAAGCAC 
CCCACGTCTC TGAACTACAG CTTCATTCAC CTGTGTTTCC AGGAGTTCTT TGCAGCAGTG 
TCCTTTGCCT TAGGGGACAT GGGGGACAAA AGTGACCATC CTAACAGCAC TGGAAGCATG 
GAAAAGTTGG TAGAAGTATA TGGGATCAAT AACCTGTTTG GGGCACCAAC CGTGCATTTC 
CTATCCGGCC TTTTGAGTGA GCAAGGAGCG ATAGAGATGG AGAACATTTT CCAATGCAAG 
CTGTCTTGGG AGAGAAAGCG GGATTCACTG CTGCAGTGGG CTGAATTGGA ACTCGGGCGT 
GAGCATTACT CTCTGCAATT GTTTCACTGT TTGTACGAGA TTCAGGACGA GGAGTTCCTG 
ACACAAGCGA TGGCCCATTT CCAAGGAACA AGAGTGTGCA TCCGAACCAG CATGGAGCTG 
CTCGTGTTCA CTTTCTGCAT TAGATTCTGC AGCCACGTGC GGCAGCTTCA ACTGAATGAG 
GGCAGACAAC ATCCAGGAGC GTGCAGGCCC ACCGACACAG TGCTGTTGAG CTGGGTCCCG 
GCCACAGAGG CCTGCTGGCA GGTTCTCTGC TCCGTTCTCC AGGTCACTGG AAACCTGAAG 
GAACTGGACC TGAGTGGAAA CATCTTGAGC CGCTCTGCAG TACAGAGTCT TGATGAGGCC 
CTGAGATGCC CTGGGTGTCA GCTAGAGACC CTGCGGCTGG CCAGCTGCGG CCTCACAGCT 
GAGAGCTGCA AGGACATTGC CTCTGGGCTG AGCTCCAGCC AAACCCTGAT GCAATTGGAA 
TTGAGCTTCA ACACACTCCT GGACGCAGGA GCTGAGCACC TTTGCCAGGG CCTGAGACAG 
CCAACCTGCA AGCTGCAGCG ACTGTTGCTG GCAGGCTGCG GCCTCACGTC TGGCTGCTGC 
CAGGACCTGG CCTCCATGCT CAGGGTCAGC CCCAGCCTGA TGGAGCTAAA CCTGCTGCAG 
AATGACCTGG ACAACCTCGG CGTGAGGCTG CTCTGTGAAG GACTCAGGCA CCCTTCCTGC 
CAGCTCATAT GCCTATGGCT GGACCAGACT CAGCTGAGCG AGGAGGTGAT GGAGATGCTG 
AAGGCCCTGG AGAGGGAGAA GCCTCAGCTG CTAGTCTCCG GGATATGGAA GCCAAGTCTG 
AGGATCCTTC ACGAGGGCCC AGGTGGAGCA GAGATGAGCG ATACATCCTC CCTCAAGCGG 
CAGAGACAAG CATCAGAGGG CAGCTCTCCT CAAGCAGCAC AGGTGAGACT CTTCTGTCCG 
TCTTCTCCTG CCCCTCTGGA GGACAAGCAC ATGGAGTCTC TGGGGACCGA GAATGACCTC 
TGGGGCCCCA CAGGGCCTGT GGCTACCCAG GCAGTTGACG AAGAGAGGAG CCTGTACCGC 
GTTCACTTGC CCGTGGCCGG CTTCTACCAC TGGCCCAACA CGGGTCTCCG CTTTGAGGTG 
AGAGGGCCAG TGACCGTTGA AATTGAATTC TGTGCCTGGG ACCAGTTCCT GAACAGGAAT 
TTCCCACAGC ACAGCTGGCT GGCTGCAGGG CCTCTGTTTG ACATCAAGGC TGAACCAGGC 
GCTGTGGCAG CCGTGTTCCT CCCTCACTTT GTGGCCTTCC GAGGGAACGA TGTGGACATC 
TCCCAGTTTC AAGTGGCCCA CTTCAAAGAG GAGGGGATGC TCCTGGAGAA GCCAGCCAGG 
GTGACGCCAT GTTACGCTGT CCTGGAAAAC CCCAGCTTCT CGCCCATGGG AGTTCTACTG 
AGGATGGTCC AAACTGCCCT GCGCTTCATT CCCATCACCT CCACTGTGTT GCTCTACCAT 
TACCTCCAGC CTGAAGAAGT TACCTTCCAC CTCTACCTGA TCCCCAGCGA TTGCTCCATT 
CAGAAGGCCA TAGACAATGA AGAAAAGAAG TTCCAGTTTG TGCGAATTCA CAAGCCACCC 
CCGCTGACCC CACTCTACAT GGGCTCCCGC TACATTGTGT CTGGTTCAGA GAAGCTGGAA 
ATACTGCCCG AGGAATTGGA GCTCTGCTAT CGGAGCCCCA GAGAATCTCA GCTGTTCTCT 
GAGTTCTACG TTCGCCATTT GAGGTCAGGG ATCAGGTTGC AAATAAGAAG CAAGAAAGAC 
TGGGACTGTG GTGTGGGAAG CCTTGGTGAA GCCAGGAGAT CTCAGACGGG CAGTTACTCC 
AGTTCCTCCA GGCCTCAGAG CCTCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_006060027.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006060089.2 Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu62103
Clone ID Related Accession (Same CDS sequence)	XM_006060027.2
Accession Version	XM_006060027.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006060027.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGCTC TGACAAGGTC TCCCAGCCCA GCAGCGATGG CCGGTGAAGC CCGGAGGCTG 
GAGGACTTGG AGGAAAAGAA GAAAGAGAAG CTGAAGGAGC TCCAGCTTTG GCTGCCTGAT 
GAGAAGCCCT GCTTGCAGTC TCCTAAGGTG GTCTATGCTC AACCAGAGAA GCTAAGGATC 
CGAAGGATCA TGATGATCCC CTCAGTGATC AAGGAGGTAC AGCACGGGTG CATGGCCATC 
CACACCAAGG GACCACACAT GAACAAGCAA GGGGCATGCA CCAAGACCCT CGAAGAGACA 
GACTTGAATT CAGCCCTGTA TCCCTCATTC TCCCACTCGC CAAGTGCACC CAACCTGGAA 
TCTCCCAGCT GGCCCACCTC CACCAAAGTG TTGACATTCT GCAAGCCTAA GCAACCAGAG 
CCCAAGGCCT CACTGAAACG GATTTTGAGC CCCCTAGTTT TCGCATCTGG ATATAGGAAA 
GACCTTTCCC CAAGCATTTA CCAAGGTTTT CCAAGTTCCC CATATCATGA GTCACCAAGC 
CAAGAGTTAC CCAATGACCC CACATCCACC GCAGTCCTGA GAGTCTGTGA AATCCCACGT 
CATTCCTGTC CAGAGCCCAG AGAGAAGGAG GGTCCTGGGA CTGTATGGCC ACTGGTTGAA 
ACATCTGGAA ACAGCTGCAC AGGAAGCAGA GAGAGATACC AGGATCAAAG GAAAGAGACA 
CCTTGCTCCA CCTGGTCATG GAAAAATGAA GATTTGCACC CAAAATTCAT GCAGTTGCTA 
CTTCTACACA CTCCTTACCA CAAAGACTAT GAGTCCTTGA ACCGGGAAAG CTGGGATCAC 
GGGGTGGTTG AGGGGCAAGG GCATTTGATT GAGGTCAGAG ACTTATTTGG CTCAGACCTG 
GGTCCCCAGG AAGGGCCTCA CACAGTCATA TTGCATGGAG TTGCTGGAAT TGGGAAGTCC 
ACACTGGCCA GGCACATAAG AAGAGCCTGG GAGGAGGGCC AGCTATACAG GAACCACTTC 
CAGCATGTCT TCTACTTCAA CTGCAGAGAG CTGGCCATGT CCAAGCTGAC AAGTCTGGAG 
GAACTCATCA CAAAAGACCA GTCCACCACT GTGGCTCCCG CTGGGCAGGT CCAGTCTCAG 
CCGGAGCAGC TGCTCTTCAT CCTCGATGGT TTAGATGAAC TGGAATGGGT CTCAGAGGAG 
CAGAGGGCAG AGCTCTGTCT GCACCGGAGC CAGCAGCAGC CCGTGCAGAC ACTGCTGGGC 
AGTTTGGTGG AGAAAACCAT ATTTCCAGAG GCATCCTTGC TGATCACAGC TCGGACTGCA 
GCTCTGCAGA AGTTCATTCC TTCTTTGAAG CAGCCATGTT GGGTGGAGGT CCTAGGGTTC 
TCTGAGTCTT CCAGGAAGGA CTATTTCTAT GAATATTTCA CAGAGGAAAG CCAAGCAACT 
AGAGCCTTTA ACGTGGTTGA ATCAAACCAA GCACTCTTGA CCATGTGTCT CACGCCCTTG 
GTGTCCTGGC TGGCCTGCAC TTGTCTGAAG CAGCAGATGG AGGCAGGGGA AGAACTCTCA 
CTGAGGTCAC AGACCATGAC AGCCCTCTAT CTGCGCTACC TTGCCCAAGC TATCCAAGCT 
CAGCCCCTGG GGACTCAGCT GAGGAGCTTC TGTACTCTTG CTGCTGAGGG TATCTGGCAA 
GGAAAGACTC TGTTCACTCC AGGGGACCTC AAGAAGCACG GATTAGATTG GGTCATCAAC 
TCCACTCCCT TAAGGATGGA TGTTCTTCAA AAGCACCCCA CGTCTCTGAA CTACAGCTTC 
ATTCACCTGT GTTTCCAGGA GTTCTTTGCA GCAGTGTCCT TTGCCTTAGG GGACATGGGG 
GACAAAAGTG ACCATCCTAA CAGCACTGGA AGCATGGAAA AGTTGGTAGA AGTATATGGG 
ATCAATAACC TGTTTGGGGC ACCAACCGTG CATTTCCTAT CCGGCCTTTT GAGTGAGCAA 
GGAGCGATAG AGATGGAGAA CATTTTCCAA TGCAAGCTGT CTTGGGAGAG AAAGCGGGAT 
TCACTGCTGC AGTGGGCTGA ATTGGAACTC GGGCGTGAGC ATTACTCTCT GCAATTGTTT 
CACTGTTTGT ACGAGATTCA GGACGAGGAG TTCCTGACAC AAGCGATGGC CCATTTCCAA 
GGAACAAGAG TGTGCATCCG AACCAGCATG GAGCTGCTCG TGTTCACTTT CTGCATTAGA 
TTCTGCAGCC ACGTGCGGCA GCTTCAACTG AATGAGGGCA GACAACATCC AGGAGCGTGC 
AGGCCCACCG ACACAGTGCT GTTGAGCTGG GTCCCGGCCA CAGAGGCCTG CTGGCAGGTT 
CTCTGCTCCG TTCTCCAGGT CACTGGAAAC CTGAAGGAAC TGGACCTGAG TGGAAACATC 
TTGAGCCGCT CTGCAGTACA GAGTCTTGAT GAGGCCCTGA GATGCCCTGG GTGTCAGCTA 
GAGACCCTGC GGCTGGCCAG CTGCGGCCTC ACAGCTGAGA GCTGCAAGGA CATTGCCTCT 
GGGCTGAGCT CCAGCCAAAC CCTGATGCAA TTGGAATTGA GCTTCAACAC ACTCCTGGAC 
GCAGGAGCTG AGCACCTTTG CCAGGGCCTG AGACAGCCAA CCTGCAAGCT GCAGCGACTG 
TTGCTGGCAG GCTGCGGCCT CACGTCTGGC TGCTGCCAGG ACCTGGCCTC CATGCTCAGG 
GTCAGCCCCA GCCTGATGGA GCTAAACCTG CTGCAGAATG ACCTGGACAA CCTCGGCGTG 
AGGCTGCTCT GTGAAGGACT CAGGCACCCT TCCTGCCAGC TCATATGCCT ATGGCTGGAC 
CAGACTCAGC TGAGCGAGGA GGTGATGGAG ATGCTGAAGG CCCTGGAGAG GGAGAAGCCT 
CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA AGTCTGAGGA TCCTTCACGA GGGCCCAGGT 
GGAGCAGAGA TGAGCGATAC ATCCTCCCTC AAGCGGCAGA GACAAGCATC AGAGGGCAGC 
TCTCCTCAAG CAGCACAGGT GAGACTCTTC TGTCCGTCTT CTCCTGCCCC TCTGGAGGAC 
AAGCACATGG AGTCTCTGGG GACCGAGAAT GACCTCTGGG GCCCCACAGG GCCTGTGGCT 
ACCCAGGCAG TTGACGAAGA GAGGAGCCTG TACCGCGTTC ACTTGCCCGT GGCCGGCTTC 
TACCACTGGC CCAACACGGG TCTCCGCTTT GAGGTGAGAG GGCCAGTGAC CGTTGAAATT 
GAATTCTGTG CCTGGGACCA GTTCCTGAAC AGGAATTTCC CACAGCACAG CTGGCTGGCT 
GCAGGGCCTC TGTTTGACAT CAAGGCTGAA CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT 
CACTTTGTGG CCTTCCGAGG GAACGATGTG GACATCTCCC AGTTTCAAGT GGCCCACTTC 
AAAGAGGAGG GGATGCTCCT GGAGAAGCCA GCCAGGGTGA CGCCATGTTA CGCTGTCCTG 
GAAAACCCCA GCTTCTCGCC CATGGGAGTT CTACTGAGGA TGGTCCAAAC TGCCCTGCGC 
TTCATTCCCA TCACCTCCAC TGTGTTGCTC TACCATTACC TCCAGCCTGA AGAAGTTACC 
TTCCACCTCT ACCTGATCCC CAGCGATTGC TCCATTCAGA AGGCCATAGA CAATGAAGAA 
AAGAAGTTCC AGTTTGTGCG AATTCACAAG CCACCCCCGC TGACCCCACT CTACATGGGC 
TCCCGCTACA TTGTGTCTGG TTCAGAGAAG CTGGAAATAC TGCCCGAGGA GATCTCAGAC 
GGGCAGTTAC TCCAGTTCCT CCAGGCCTCA GAGCCTCACC TGACTCCCCA GCTGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006060089.2
CDS	245..4084
Translation	MPALTRSPSPAAMAGEARRLEDLEEKKKEKLKELQLWLPDEKPCLQSPKVVYAQPEKLRIRRIMMIPSVIKEVQHGCMAIHTKGPHMNKQGACTKTLEETDLNSALYPSFSHSPSAPNLESPSWPTSTKVLTFCKPKQPEPKASLKRILSPLVFASGYRKDLSPSIYQGFPSSPYHESPSQELPNDPTSTAVLRVCEIPRHSCPEPREKEGPGTVWPLVETSGNSCTGSRERYQDQRKETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGPQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELAMSKLTSLEELITKDQSTTVAPAGQVQSQPEQLLFILDGLDELEWVSEEQRAELCLHRSQQQPVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFNVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLRSQTMTALYLRYLAQAIQAQPLGTQLRSFCTLAAEGIWQGKTLFTPGDLKKHGLDWVINSTPLRMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDMGDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAIEMENIFQCKLSWERKRDSLLQWAELELGREHYSLQLFHCLYEIQDEEFLTQAMAHFQGTRVCIRTSMELLVFTFCIRFCSHVRQLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSGNILSRSAVQSLDEALRCPGCQLETLRLASCGLTAESCKDIASGLSSSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDNLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVMEMLKALEREKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPLEDKHMESLGTENDLWGPTGPVATQAVDEERSLYRVHLPVAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQHSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKKFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEEISDGQLLQFLQASEPHLTPQLP

Target ORF information:

RefSeq Version	XM_006060027.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006060027.2

ORF Insert Sequence:

ATGCCTGCTC TGACAAGGTC TCCCAGCCCA GCAGCGATGG CCGGTGAAGC CCGGAGGCTG 
GAGGACTTGG AGGAAAAGAA GAAAGAGAAG CTGAAGGAGC TCCAGCTTTG GCTGCCTGAT 
GAGAAGCCCT GCTTGCAGTC TCCTAAGGTG GTCTATGCTC AACCAGAGAA GCTAAGGATC 
CGAAGGATCA TGATGATCCC CTCAGTGATC AAGGAGGTAC AGCACGGGTG CATGGCCATC 
CACACCAAGG GACCACACAT GAACAAGCAA GGGGCATGCA CCAAGACCCT CGAAGAGACA 
GACTTGAATT CAGCCCTGTA TCCCTCATTC TCCCACTCGC CAAGTGCACC CAACCTGGAA 
TCTCCCAGCT GGCCCACCTC CACCAAAGTG TTGACATTCT GCAAGCCTAA GCAACCAGAG 
CCCAAGGCCT CACTGAAACG GATTTTGAGC CCCCTAGTTT TCGCATCTGG ATATAGGAAA 
GACCTTTCCC CAAGCATTTA CCAAGGTTTT CCAAGTTCCC CATATCATGA GTCACCAAGC 
CAAGAGTTAC CCAATGACCC CACATCCACC GCAGTCCTGA GAGTCTGTGA AATCCCACGT 
CATTCCTGTC CAGAGCCCAG AGAGAAGGAG GGTCCTGGGA CTGTATGGCC ACTGGTTGAA 
ACATCTGGAA ACAGCTGCAC AGGAAGCAGA GAGAGATACC AGGATCAAAG GAAAGAGACA 
CCTTGCTCCA CCTGGTCATG GAAAAATGAA GATTTGCACC CAAAATTCAT GCAGTTGCTA 
CTTCTACACA CTCCTTACCA CAAAGACTAT GAGTCCTTGA ACCGGGAAAG CTGGGATCAC 
GGGGTGGTTG AGGGGCAAGG GCATTTGATT GAGGTCAGAG ACTTATTTGG CTCAGACCTG 
GGTCCCCAGG AAGGGCCTCA CACAGTCATA TTGCATGGAG TTGCTGGAAT TGGGAAGTCC 
ACACTGGCCA GGCACATAAG AAGAGCCTGG GAGGAGGGCC AGCTATACAG GAACCACTTC 
CAGCATGTCT TCTACTTCAA CTGCAGAGAG CTGGCCATGT CCAAGCTGAC AAGTCTGGAG 
GAACTCATCA CAAAAGACCA GTCCACCACT GTGGCTCCCG CTGGGCAGGT CCAGTCTCAG 
CCGGAGCAGC TGCTCTTCAT CCTCGATGGT TTAGATGAAC TGGAATGGGT CTCAGAGGAG 
CAGAGGGCAG AGCTCTGTCT GCACCGGAGC CAGCAGCAGC CCGTGCAGAC ACTGCTGGGC 
AGTTTGGTGG AGAAAACCAT ATTTCCAGAG GCATCCTTGC TGATCACAGC TCGGACTGCA 
GCTCTGCAGA AGTTCATTCC TTCTTTGAAG CAGCCATGTT GGGTGGAGGT CCTAGGGTTC 
TCTGAGTCTT CCAGGAAGGA CTATTTCTAT GAATATTTCA CAGAGGAAAG CCAAGCAACT 
AGAGCCTTTA ACGTGGTTGA ATCAAACCAA GCACTCTTGA CCATGTGTCT CACGCCCTTG 
GTGTCCTGGC TGGCCTGCAC TTGTCTGAAG CAGCAGATGG AGGCAGGGGA AGAACTCTCA 
CTGAGGTCAC AGACCATGAC AGCCCTCTAT CTGCGCTACC TTGCCCAAGC TATCCAAGCT 
CAGCCCCTGG GGACTCAGCT GAGGAGCTTC TGTACTCTTG CTGCTGAGGG TATCTGGCAA 
GGAAAGACTC TGTTCACTCC AGGGGACCTC AAGAAGCACG GATTAGATTG GGTCATCAAC 
TCCACTCCCT TAAGGATGGA TGTTCTTCAA AAGCACCCCA CGTCTCTGAA CTACAGCTTC 
ATTCACCTGT GTTTCCAGGA GTTCTTTGCA GCAGTGTCCT TTGCCTTAGG GGACATGGGG 
GACAAAAGTG ACCATCCTAA CAGCACTGGA AGCATGGAAA AGTTGGTAGA AGTATATGGG 
ATCAATAACC TGTTTGGGGC ACCAACCGTG CATTTCCTAT CCGGCCTTTT GAGTGAGCAA 
GGAGCGATAG AGATGGAGAA CATTTTCCAA TGCAAGCTGT CTTGGGAGAG AAAGCGGGAT 
TCACTGCTGC AGTGGGCTGA ATTGGAACTC GGGCGTGAGC ATTACTCTCT GCAATTGTTT 
CACTGTTTGT ACGAGATTCA GGACGAGGAG TTCCTGACAC AAGCGATGGC CCATTTCCAA 
GGAACAAGAG TGTGCATCCG AACCAGCATG GAGCTGCTCG TGTTCACTTT CTGCATTAGA 
TTCTGCAGCC ACGTGCGGCA GCTTCAACTG AATGAGGGCA GACAACATCC AGGAGCGTGC 
AGGCCCACCG ACACAGTGCT GTTGAGCTGG GTCCCGGCCA CAGAGGCCTG CTGGCAGGTT 
CTCTGCTCCG TTCTCCAGGT CACTGGAAAC CTGAAGGAAC TGGACCTGAG TGGAAACATC 
TTGAGCCGCT CTGCAGTACA GAGTCTTGAT GAGGCCCTGA GATGCCCTGG GTGTCAGCTA 
GAGACCCTGC GGCTGGCCAG CTGCGGCCTC ACAGCTGAGA GCTGCAAGGA CATTGCCTCT 
GGGCTGAGCT CCAGCCAAAC CCTGATGCAA TTGGAATTGA GCTTCAACAC ACTCCTGGAC 
GCAGGAGCTG AGCACCTTTG CCAGGGCCTG AGACAGCCAA CCTGCAAGCT GCAGCGACTG 
TTGCTGGCAG GCTGCGGCCT CACGTCTGGC TGCTGCCAGG ACCTGGCCTC CATGCTCAGG 
GTCAGCCCCA GCCTGATGGA GCTAAACCTG CTGCAGAATG ACCTGGACAA CCTCGGCGTG 
AGGCTGCTCT GTGAAGGACT CAGGCACCCT TCCTGCCAGC TCATATGCCT ATGGCTGGAC 
CAGACTCAGC TGAGCGAGGA GGTGATGGAG ATGCTGAAGG CCCTGGAGAG GGAGAAGCCT 
CAGCTGCTAG TCTCCGGGAT ATGGAAGCCA AGTCTGAGGA TCCTTCACGA GGGCCCAGGT 
GGAGCAGAGA TGAGCGATAC ATCCTCCCTC AAGCGGCAGA GACAAGCATC AGAGGGCAGC 
TCTCCTCAAG CAGCACAGGT GAGACTCTTC TGTCCGTCTT CTCCTGCCCC TCTGGAGGAC 
AAGCACATGG AGTCTCTGGG GACCGAGAAT GACCTCTGGG GCCCCACAGG GCCTGTGGCT 
ACCCAGGCAG TTGACGAAGA GAGGAGCCTG TACCGCGTTC ACTTGCCCGT GGCCGGCTTC 
TACCACTGGC CCAACACGGG TCTCCGCTTT GAGGTGAGAG GGCCAGTGAC CGTTGAAATT 
GAATTCTGTG CCTGGGACCA GTTCCTGAAC AGGAATTTCC CACAGCACAG CTGGCTGGCT 
GCAGGGCCTC TGTTTGACAT CAAGGCTGAA CCAGGCGCTG TGGCAGCCGT GTTCCTCCCT 
CACTTTGTGG CCTTCCGAGG GAACGATGTG GACATCTCCC AGTTTCAAGT GGCCCACTTC 
AAAGAGGAGG GGATGCTCCT GGAGAAGCCA GCCAGGGTGA CGCCATGTTA CGCTGTCCTG 
GAAAACCCCA GCTTCTCGCC CATGGGAGTT CTACTGAGGA TGGTCCAAAC TGCCCTGCGC 
TTCATTCCCA TCACCTCCAC TGTGTTGCTC TACCATTACC TCCAGCCTGA AGAAGTTACC 
TTCCACCTCT ACCTGATCCC CAGCGATTGC TCCATTCAGA AGGCCATAGA CAATGAAGAA 
AAGAAGTTCC AGTTTGTGCG AATTCACAAG CCACCCCCGC TGACCCCACT CTACATGGGC 
TCCCGCTACA TTGTGTCTGG TTCAGAGAAG CTGGAAATAC TGCCCGAGGA GATCTCAGAC 
GGGCAGTTAC TCCAGTTCCT CCAGGCCTCA GAGCCTCACC TGACTCCCCA GCTGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_025280775.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025136560.1 Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X4, mRNA.

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CloneID	OBu62104
Clone ID Related Accession (Same CDS sequence)	XM_025280775.1
Accession Version	XM_025280775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGCTC TGACAAGGTC TCCCAGCCCA GCAGCGATGG CCGGTGAAGC CCGGAGGCTG 
GAGGACTTGG AGGAAAAGAA GAAAGAGAAG CTGAAGGAGC TCCAGCTTTG GCTGCCTGAT 
GAGAAGCCCT GCTTGCAGTC TCCTAAGGTG GTCTATGCTC AACCAGAGAA GCTAAGGATC 
CGAAGGATCA TGATGATCCC CTCAGTGATC AAGGAGGTAC AGCACGGGTG CATGGCCATC 
CACACCAAGG GACCACACAT GAACAAGCAA GGGGCATGCA CCAAGACCCT CGAAGAGACA 
GACTTGAATT CAGACCTTTC CCCAAGCATT TACCAAGGTT TTCCAAGTTC CCCATATCAT 
GAGTCACCAA GCCAAGAGTT ACCCAATGAC CCCACATCCA CCGCAGTCCT GAGAGTCTGT 
GAAATCCCAC GTCATTCCTG TCCAGAGCCC AGAGAGAAGG AGGGTCCTGG GACTGTATGG 
CCACTGGTTG AAACATCTGG AAACAGCTGC ACAGGAAGCA GAGAGAGATA CCAGGATCAA 
AGGAAAGAGA CACCTTGCTC CACCTGGTCA TGGAAAAATG AAGATTTGCA CCCAAAATTC 
ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ATGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTCCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAT GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGGCAG 
GTCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGG 
GTCTCAGAGG AGCAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GCCCGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTAGGGT TCTCTGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAACGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGT 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGTCTGA AGCAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAGGTC ACAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGGACTCAG CTGAGGAGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGGC AAGGAAAGAC TCTGTTCACT CCAGGGGACC TCAAGAAGCA CGGATTAGAT 
TGGGTCATCA ACTCCACTCC CTTAAGGATG GATGTTCTTC AAAAGCACCC CACGTCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATGG GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTGTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAT AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG ATTCACTGCT GCAGTGGGCT GAATTGGAAC TCGGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAACAAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGCGG CAGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCGT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAGGCC 
TGCTGGCAGG TTCTCTGCTC CGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTGGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGTCAGC TAGAGACCCT GCGGCTGGCC AGCTGCGGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CTCCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
AACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGATGG AGATGCTGAA GGCCCTGGAG 
AGGGAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGCGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGACTCT TCTGTCCGTC TTCTCCTGCC 
CCTCTGGAGG ACAAGCACAT GGAGTCTCTG GGGACCGAGA ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCCAGGC AGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
GTGGCCGGCT TCTACCACTG GCCCAACACG GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCAC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCTGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGAAGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGACTG GGACTGTGGT 
GTGGGAAGCC TTGGTGAAGC CAGGAGATCT CAGACGGGCA GTTACTCCAG TTCCTCCAGG 
CCTCAGAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025136560.1
CDS	244..4041
Translation	MPALTRSPSPAAMAGEARRLEDLEEKKKEKLKELQLWLPDEKPCLQSPKVVYAQPEKLRIRRIMMIPSVIKEVQHGCMAIHTKGPHMNKQGACTKTLEETDLNSDLSPSIYQGFPSSPYHESPSQELPNDPTSTAVLRVCEIPRHSCPEPREKEGPGTVWPLVETSGNSCTGSRERYQDQRKETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGPQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELAMSKLTSLEELITKDQSTTVAPAGQVQSQPEQLLFILDGLDELEWVSEEQRAELCLHRSQQQPVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFNVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLRSQTMTALYLRYLAQAIQAQPLGTQLRSFCTLAAEGIWQGKTLFTPGDLKKHGLDWVINSTPLRMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDMGDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAIEMENIFQCKLSWERKRDSLLQWAELELGREHYSLQLFHCLYEIQDEEFLTQAMAHFQGTRVCIRTSMELLVFTFCIRFCSHVRQLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSGNILSRSAVQSLDEALRCPGCQLETLRLASCGLTAESCKDIASGLSSSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDNLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVMEMLKALEREKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPLEDKHMESLGTENDLWGPTGPVATQAVDEERSLYRVHLPVAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQHSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKKFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDWDCGVGSLGEARRSQTGSYSSSSRPQSLT

Target ORF information:

RefSeq Version	XM_025280775.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025280775.1

ORF Insert Sequence:

ATGCCTGCTC TGACAAGGTC TCCCAGCCCA GCAGCGATGG CCGGTGAAGC CCGGAGGCTG 
GAGGACTTGG AGGAAAAGAA GAAAGAGAAG CTGAAGGAGC TCCAGCTTTG GCTGCCTGAT 
GAGAAGCCCT GCTTGCAGTC TCCTAAGGTG GTCTATGCTC AACCAGAGAA GCTAAGGATC 
CGAAGGATCA TGATGATCCC CTCAGTGATC AAGGAGGTAC AGCACGGGTG CATGGCCATC 
CACACCAAGG GACCACACAT GAACAAGCAA GGGGCATGCA CCAAGACCCT CGAAGAGACA 
GACTTGAATT CAGACCTTTC CCCAAGCATT TACCAAGGTT TTCCAAGTTC CCCATATCAT 
GAGTCACCAA GCCAAGAGTT ACCCAATGAC CCCACATCCA CCGCAGTCCT GAGAGTCTGT 
GAAATCCCAC GTCATTCCTG TCCAGAGCCC AGAGAGAAGG AGGGTCCTGG GACTGTATGG 
CCACTGGTTG AAACATCTGG AAACAGCTGC ACAGGAAGCA GAGAGAGATA CCAGGATCAA 
AGGAAAGAGA CACCTTGCTC CACCTGGTCA TGGAAAAATG AAGATTTGCA CCCAAAATTC 
ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ATGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTCCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAT GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGGCAG 
GTCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGG 
GTCTCAGAGG AGCAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GCCCGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTAGGGT TCTCTGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAACGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGT 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGTCTGA AGCAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAGGTC ACAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGGACTCAG CTGAGGAGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGGC AAGGAAAGAC TCTGTTCACT CCAGGGGACC TCAAGAAGCA CGGATTAGAT 
TGGGTCATCA ACTCCACTCC CTTAAGGATG GATGTTCTTC AAAAGCACCC CACGTCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATGG GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTGTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAT AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG ATTCACTGCT GCAGTGGGCT GAATTGGAAC TCGGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAACAAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGCGG CAGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCGT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAGGCC 
TGCTGGCAGG TTCTCTGCTC CGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTGGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGTCAGC TAGAGACCCT GCGGCTGGCC AGCTGCGGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CTCCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
AACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGATGG AGATGCTGAA GGCCCTGGAG 
AGGGAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGCGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGACTCT TCTGTCCGTC TTCTCCTGCC 
CCTCTGGAGG ACAAGCACAT GGAGTCTCTG GGGACCGAGA ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCCAGGC AGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
GTGGCCGGCT TCTACCACTG GCCCAACACG GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCAC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCTGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGAAGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGACTG GGACTGTGGT 
GTGGGAAGCC TTGGTGAAGC CAGGAGATCT CAGACGGGCA GTTACTCCAG TTCCTCCAGG 
CCTCAGAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_006060026.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006060088.2 Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu62105
Clone ID Related Accession (Same CDS sequence)	XM_006060026.2
Accession Version	XM_006060026.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006060026.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGCTC TGACAAGGTC TCCCAGCCCA GCAGCGATGG CCGGTGAAGC CCGGAGGCTG 
GAGGACTTGG AGGAAAAGAA GAAAGAGAAG CTGAAGGAGC TCCAGCTTTG GCTGCCTGAT 
GAGAAGCCCT GCTTGCAGTC TCCTAAGGTG GTCTATGCTC AACCAGAGAA GCTAAGGATC 
CGAAGGATCA TGATGATCCC CTCAGTGATC AAGGAGGTAC AGCACGGGTG CATGGCCATC 
CACACCAAGG GACCACACAT GAACAAGCAA GGGGCATGCA CCAAGACCCT CGAAGAGACA 
GACTTGAATT CAGCCCTGTA TCCCTCATTC TCCCACTCGC CAAGTGCACC CAACCTGGAA 
TCTCCCAGCT GGCCCACCTC CACCAAAGTG TTGACATTCT GCAAGCCTAA GCAACCAGAG 
CCCAAGGCCT CACTGAAACG GATTTTGAGC CCCCTAGTTT TCGCATCTGG ATATAGGAAA 
GACCTTTCCC CAAGCATTTA CCAAGGTTTT CCAAGTTCCC CATATCATGA GTCACCAAGC 
CAAGAGTTAC CCAATGACCC CACATCCACC GCAGTCCTGA GAGTCTGTGA AATCCCACGT 
CATTCCTGTC CAGAGCCCAG AGAGAAGGAG GGTCCTGGGA CTGTATGGCC ACTGGTTGAA 
ACATCTGGAA ACAGCTGCAC AGGAAGCAGA GAGAGATACC AGGATCAAAG GAAAGAGACA 
CCTTGCTCCA CCTGGTCATG GAAAAATGAA GATTTGCACC CAAAATTCAT GCAGTTGCTA 
CTTCTACACA CTCCTTACCA CAAAGACTAT GAGTCCTTGA ACCGGGAAAG CTGGGATCAC 
GGGGTGGTTG AGGGGCAAGG GCATTTGATT GAGGTCAGAG ACTTATTTGG CTCAGACCTG 
GGTCCCCAGG AAGGGCCTCA CACAGTCATA TTGCATGGAG TTGCTGGAAT TGGGAAGTCC 
ACACTGGCCA GGCACATAAG AAGAGCCTGG GAGGAGGGCC AGCTATACAG GAACCACTTC 
CAGCATGTCT TCTACTTCAA CTGCAGAGAG CTGGCCATGT CCAAGCTGAC AAGTCTGGAG 
GAACTCATCA CAAAAGACCA GTCCACCACT GTGGCTCCCG CTGGGCAGGT CCAGTCTCAG 
CCGGAGCAGC TGCTCTTCAT CCTCGATGGT TTAGATGAAC TGGAATGGGT CTCAGAGGAG 
CAGAGGGCAG AGCTCTGTCT GCACCGGAGC CAGCAGCAGC CCGTGCAGAC ACTGCTGGGC 
AGTTTGGTGG AGAAAACCAT ATTTCCAGAG GCATCCTTGC TGATCACAGC TCGGACTGCA 
GCTCTGCAGA AGTTCATTCC TTCTTTGAAG CAGCCATGTT GGGTGGAGGT CCTAGGGTTC 
TCTGAGTCTT CCAGGAAGGA CTATTTCTAT GAATATTTCA CAGAGGAAAG CCAAGCAACT 
AGAGCCTTTA ACGTGGTTGA ATCAAACCAA GCACTCTTGA CCATGTGTCT CACGCCCTTG 
GTGTCCTGGC TGGCCTGCAC TTGTCTGAAG CAGCAGATGG AGGCAGGGGA AGAACTCTCA 
CTGAGGTCAC AGACCATGAC AGCCCTCTAT CTGCGCTACC TTGCCCAAGC TATCCAAGCT 
CAGCCCCTGG GGACTCAGCT GAGGAGCTTC TGTACTCTTG CTGCTGAGGG TATCTGGCAA 
GGAAAGACTC TGTTCACTCC AGGGGACCTC AAGAAGCACG GATTAGATTG GGTCATCAAC 
TCCACTCCCT TAAGGATGGA TGTTCTTCAA AAGCACCCCA CGTCTCTGAA CTACAGCTTC 
ATTCACCTGT GTTTCCAGGA GTTCTTTGCA GCAGTGTCCT TTGCCTTAGG GGACATGGGG 
GACAAAAGTG ACCATCCTAA CAGCACTGGA AGCATGGAAA AGTTGGTAGA AGTATATGGG 
ATCAATAACC TGTTTGGGGC ACCAACCGTG CATTTCCTAT CCGGCCTTTT GAGTGAGCAA 
GGAGCGATAG AGATGGAGAA CATTTTCCAA TGCAAGCTGT CTTGGGAGAG AAAGCGGGAT 
TCACTGCTGC AGTGGGCTGA ATTGGAACTC GGGCGTGAGC ATTACTCTCT GCAATTGTTT 
CACTGTTTGT ACGAGATTCA GGACGAGGAG TTCCTGACAC AAGCGATGGC CCATTTCCAA 
GGAACAAGAG TGTGCATCCG AACCAGCATG GAGCTGCTCG TGTTCACTTT CTGCATTAGA 
TTCTGCAGCC ACGTGCGGCA GCTTCAACTG AATGAGGGCA GACAACATCC AGGAGCGTGC 
AGGCCCACCG ACACAGTGCT GTTGAGCTGG GTCCCGGCCA CAGAGGCCTG CTGGCAGGTT 
CTCTGCTCCG TTCTCCAGGT CACTGGAAAC CTGAAGGAAC TGGACCTGAG TGGAAACATC 
TTGAGCCGCT CTGCAGTACA GAGTCTTGAT GAGGCCCTGA GATGCCCTGG GTGTCAGCTA 
GAGACCCTGC GGCTGGCCAG CTGCGGCCTC ACAGCTGAGA GCTGCAAGGA CATTGCCTCT 
GGGCTGAGCT CCAGCCAAAC CCTGATGCAA TTGGAATTGA GCTTCAACAC ACTCCTGGAC 
GCAGGAGCTG AGCACCTTTG CCAGGGCCTG AGACAGCCAA CCTGCAAGCT GCAGCGACTG 
TTGCTGGACC AGACTCAGCT GAGCGAGGAG GTGATGGAGA TGCTGAAGGC CCTGGAGAGG 
GAGAAGCCTC AGCTGCTAGT CTCCGGGATA TGGAAGCCAA GTCTGAGGAT CCTTCACGAG 
GGCCCAGGTG GAGCAGAGAT GAGCGATACA TCCTCCCTCA AGCGGCAGAG ACAAGCATCA 
GAGGGCAGCT CTCCTCAAGC AGCACAGGTG AGACTCTTCT GTCCGTCTTC TCCTGCCCCT 
CTGGAGGACA AGCACATGGA GTCTCTGGGG ACCGAGAATG ACCTCTGGGG CCCCACAGGG 
CCTGTGGCTA CCCAGGCAGT TGACGAAGAG AGGAGCCTGT ACCGCGTTCA CTTGCCCGTG 
GCCGGCTTCT ACCACTGGCC CAACACGGGT CTCCGCTTTG AGGTGAGAGG GCCAGTGACC 
GTTGAAATTG AATTCTGTGC CTGGGACCAG TTCCTGAACA GGAATTTCCC ACAGCACAGC 
TGGCTGGCTG CAGGGCCTCT GTTTGACATC AAGGCTGAAC CAGGCGCTGT GGCAGCCGTG 
TTCCTCCCTC ACTTTGTGGC CTTCCGAGGG AACGATGTGG ACATCTCCCA GTTTCAAGTG 
GCCCACTTCA AAGAGGAGGG GATGCTCCTG GAGAAGCCAG CCAGGGTGAC GCCATGTTAC 
GCTGTCCTGG AAAACCCCAG CTTCTCGCCC ATGGGAGTTC TACTGAGGAT GGTCCAAACT 
GCCCTGCGCT TCATTCCCAT CACCTCCACT GTGTTGCTCT ACCATTACCT CCAGCCTGAA 
GAAGTTACCT TCCACCTCTA CCTGATCCCC AGCGATTGCT CCATTCAGAA GGCCATAGAC 
AATGAAGAAA AGAAGTTCCA GTTTGTGCGA ATTCACAAGC CACCCCCGCT GACCCCACTC 
TACATGGGCT CCCGCTACAT TGTGTCTGGT TCAGAGAAGC TGGAAATACT GCCCGAGGAA 
TTGGAGCTCT GCTATCGGAG CCCCAGAGAA TCTCAGCTGT TCTCTGAGTT CTACGTTCGC 
CATTTGAGGT CAGGGATCAG GTTGCAAATA AGAAGCAAGA AAGACTGGGA CTGTGGTGTG 
GGAAGCCTTG GTGAAGCCAG GAGATCTCAG ACGGGCAGTT ACTCCAGTTC CTCCAGGCCT 
CAGAGCCTCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006060088.2
CDS	244..4038
Translation	MPALTRSPSPAAMAGEARRLEDLEEKKKEKLKELQLWLPDEKPCLQSPKVVYAQPEKLRIRRIMMIPSVIKEVQHGCMAIHTKGPHMNKQGACTKTLEETDLNSALYPSFSHSPSAPNLESPSWPTSTKVLTFCKPKQPEPKASLKRILSPLVFASGYRKDLSPSIYQGFPSSPYHESPSQELPNDPTSTAVLRVCEIPRHSCPEPREKEGPGTVWPLVETSGNSCTGSRERYQDQRKETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGPQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELAMSKLTSLEELITKDQSTTVAPAGQVQSQPEQLLFILDGLDELEWVSEEQRAELCLHRSQQQPVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFNVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLRSQTMTALYLRYLAQAIQAQPLGTQLRSFCTLAAEGIWQGKTLFTPGDLKKHGLDWVINSTPLRMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDMGDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAIEMENIFQCKLSWERKRDSLLQWAELELGREHYSLQLFHCLYEIQDEEFLTQAMAHFQGTRVCIRTSMELLVFTFCIRFCSHVRQLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSGNILSRSAVQSLDEALRCPGCQLETLRLASCGLTAESCKDIASGLSSSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLDQTQLSEEVMEMLKALEREKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPLEDKHMESLGTENDLWGPTGPVATQAVDEERSLYRVHLPVAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQHSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKKFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDWDCGVGSLGEARRSQTGSYSSSSRPQSLT

Target ORF information:

RefSeq Version	XM_006060026.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006060026.2

ORF Insert Sequence:

ATGCCTGCTC TGACAAGGTC TCCCAGCCCA GCAGCGATGG CCGGTGAAGC CCGGAGGCTG 
GAGGACTTGG AGGAAAAGAA GAAAGAGAAG CTGAAGGAGC TCCAGCTTTG GCTGCCTGAT 
GAGAAGCCCT GCTTGCAGTC TCCTAAGGTG GTCTATGCTC AACCAGAGAA GCTAAGGATC 
CGAAGGATCA TGATGATCCC CTCAGTGATC AAGGAGGTAC AGCACGGGTG CATGGCCATC 
CACACCAAGG GACCACACAT GAACAAGCAA GGGGCATGCA CCAAGACCCT CGAAGAGACA 
GACTTGAATT CAGCCCTGTA TCCCTCATTC TCCCACTCGC CAAGTGCACC CAACCTGGAA 
TCTCCCAGCT GGCCCACCTC CACCAAAGTG TTGACATTCT GCAAGCCTAA GCAACCAGAG 
CCCAAGGCCT CACTGAAACG GATTTTGAGC CCCCTAGTTT TCGCATCTGG ATATAGGAAA 
GACCTTTCCC CAAGCATTTA CCAAGGTTTT CCAAGTTCCC CATATCATGA GTCACCAAGC 
CAAGAGTTAC CCAATGACCC CACATCCACC GCAGTCCTGA GAGTCTGTGA AATCCCACGT 
CATTCCTGTC CAGAGCCCAG AGAGAAGGAG GGTCCTGGGA CTGTATGGCC ACTGGTTGAA 
ACATCTGGAA ACAGCTGCAC AGGAAGCAGA GAGAGATACC AGGATCAAAG GAAAGAGACA 
CCTTGCTCCA CCTGGTCATG GAAAAATGAA GATTTGCACC CAAAATTCAT GCAGTTGCTA 
CTTCTACACA CTCCTTACCA CAAAGACTAT GAGTCCTTGA ACCGGGAAAG CTGGGATCAC 
GGGGTGGTTG AGGGGCAAGG GCATTTGATT GAGGTCAGAG ACTTATTTGG CTCAGACCTG 
GGTCCCCAGG AAGGGCCTCA CACAGTCATA TTGCATGGAG TTGCTGGAAT TGGGAAGTCC 
ACACTGGCCA GGCACATAAG AAGAGCCTGG GAGGAGGGCC AGCTATACAG GAACCACTTC 
CAGCATGTCT TCTACTTCAA CTGCAGAGAG CTGGCCATGT CCAAGCTGAC AAGTCTGGAG 
GAACTCATCA CAAAAGACCA GTCCACCACT GTGGCTCCCG CTGGGCAGGT CCAGTCTCAG 
CCGGAGCAGC TGCTCTTCAT CCTCGATGGT TTAGATGAAC TGGAATGGGT CTCAGAGGAG 
CAGAGGGCAG AGCTCTGTCT GCACCGGAGC CAGCAGCAGC CCGTGCAGAC ACTGCTGGGC 
AGTTTGGTGG AGAAAACCAT ATTTCCAGAG GCATCCTTGC TGATCACAGC TCGGACTGCA 
GCTCTGCAGA AGTTCATTCC TTCTTTGAAG CAGCCATGTT GGGTGGAGGT CCTAGGGTTC 
TCTGAGTCTT CCAGGAAGGA CTATTTCTAT GAATATTTCA CAGAGGAAAG CCAAGCAACT 
AGAGCCTTTA ACGTGGTTGA ATCAAACCAA GCACTCTTGA CCATGTGTCT CACGCCCTTG 
GTGTCCTGGC TGGCCTGCAC TTGTCTGAAG CAGCAGATGG AGGCAGGGGA AGAACTCTCA 
CTGAGGTCAC AGACCATGAC AGCCCTCTAT CTGCGCTACC TTGCCCAAGC TATCCAAGCT 
CAGCCCCTGG GGACTCAGCT GAGGAGCTTC TGTACTCTTG CTGCTGAGGG TATCTGGCAA 
GGAAAGACTC TGTTCACTCC AGGGGACCTC AAGAAGCACG GATTAGATTG GGTCATCAAC 
TCCACTCCCT TAAGGATGGA TGTTCTTCAA AAGCACCCCA CGTCTCTGAA CTACAGCTTC 
ATTCACCTGT GTTTCCAGGA GTTCTTTGCA GCAGTGTCCT TTGCCTTAGG GGACATGGGG 
GACAAAAGTG ACCATCCTAA CAGCACTGGA AGCATGGAAA AGTTGGTAGA AGTATATGGG 
ATCAATAACC TGTTTGGGGC ACCAACCGTG CATTTCCTAT CCGGCCTTTT GAGTGAGCAA 
GGAGCGATAG AGATGGAGAA CATTTTCCAA TGCAAGCTGT CTTGGGAGAG AAAGCGGGAT 
TCACTGCTGC AGTGGGCTGA ATTGGAACTC GGGCGTGAGC ATTACTCTCT GCAATTGTTT 
CACTGTTTGT ACGAGATTCA GGACGAGGAG TTCCTGACAC AAGCGATGGC CCATTTCCAA 
GGAACAAGAG TGTGCATCCG AACCAGCATG GAGCTGCTCG TGTTCACTTT CTGCATTAGA 
TTCTGCAGCC ACGTGCGGCA GCTTCAACTG AATGAGGGCA GACAACATCC AGGAGCGTGC 
AGGCCCACCG ACACAGTGCT GTTGAGCTGG GTCCCGGCCA CAGAGGCCTG CTGGCAGGTT 
CTCTGCTCCG TTCTCCAGGT CACTGGAAAC CTGAAGGAAC TGGACCTGAG TGGAAACATC 
TTGAGCCGCT CTGCAGTACA GAGTCTTGAT GAGGCCCTGA GATGCCCTGG GTGTCAGCTA 
GAGACCCTGC GGCTGGCCAG CTGCGGCCTC ACAGCTGAGA GCTGCAAGGA CATTGCCTCT 
GGGCTGAGCT CCAGCCAAAC CCTGATGCAA TTGGAATTGA GCTTCAACAC ACTCCTGGAC 
GCAGGAGCTG AGCACCTTTG CCAGGGCCTG AGACAGCCAA CCTGCAAGCT GCAGCGACTG 
TTGCTGGACC AGACTCAGCT GAGCGAGGAG GTGATGGAGA TGCTGAAGGC CCTGGAGAGG 
GAGAAGCCTC AGCTGCTAGT CTCCGGGATA TGGAAGCCAA GTCTGAGGAT CCTTCACGAG 
GGCCCAGGTG GAGCAGAGAT GAGCGATACA TCCTCCCTCA AGCGGCAGAG ACAAGCATCA 
GAGGGCAGCT CTCCTCAAGC AGCACAGGTG AGACTCTTCT GTCCGTCTTC TCCTGCCCCT 
CTGGAGGACA AGCACATGGA GTCTCTGGGG ACCGAGAATG ACCTCTGGGG CCCCACAGGG 
CCTGTGGCTA CCCAGGCAGT TGACGAAGAG AGGAGCCTGT ACCGCGTTCA CTTGCCCGTG 
GCCGGCTTCT ACCACTGGCC CAACACGGGT CTCCGCTTTG AGGTGAGAGG GCCAGTGACC 
GTTGAAATTG AATTCTGTGC CTGGGACCAG TTCCTGAACA GGAATTTCCC ACAGCACAGC 
TGGCTGGCTG CAGGGCCTCT GTTTGACATC AAGGCTGAAC CAGGCGCTGT GGCAGCCGTG 
TTCCTCCCTC ACTTTGTGGC CTTCCGAGGG AACGATGTGG ACATCTCCCA GTTTCAAGTG 
GCCCACTTCA AAGAGGAGGG GATGCTCCTG GAGAAGCCAG CCAGGGTGAC GCCATGTTAC 
GCTGTCCTGG AAAACCCCAG CTTCTCGCCC ATGGGAGTTC TACTGAGGAT GGTCCAAACT 
GCCCTGCGCT TCATTCCCAT CACCTCCACT GTGTTGCTCT ACCATTACCT CCAGCCTGAA 
GAAGTTACCT TCCACCTCTA CCTGATCCCC AGCGATTGCT CCATTCAGAA GGCCATAGAC 
AATGAAGAAA AGAAGTTCCA GTTTGTGCGA ATTCACAAGC CACCCCCGCT GACCCCACTC 
TACATGGGCT CCCGCTACAT TGTGTCTGGT TCAGAGAAGC TGGAAATACT GCCCGAGGAA 
TTGGAGCTCT GCTATCGGAG CCCCAGAGAA TCTCAGCTGT TCTCTGAGTT CTACGTTCGC 
CATTTGAGGT CAGGGATCAG GTTGCAAATA AGAAGCAAGA AAGACTGGGA CTGTGGTGTG 
GGAAGCCTTG GTGAAGCCAG GAGATCTCAG ACGGGCAGTT ACTCCAGTTC CTCCAGGCCT 
CAGAGCCTCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_025280776.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025136561.1 Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBu62106
Clone ID Related Accession (Same CDS sequence)	XM_025280776.1
Accession Version	XM_025280776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGCTC TGACAAGGTC TCCCAGCCCA GCAGCGATGG CCGGTGAAGC CCGGAGGCTG 
GAGGACTTGG AGGAAAAGAA GAAAGAGAAG CTGAAGGAGC TCCAGCTTTG GCTGCCTGAT 
GAGAAGCCCT GCTTGCAGTC TCCTAAGGTG GTCTATGCTC AACCAGAGAA GCTAAGGATC 
CGAAGGATCA TGATGATCCC CTCAGTGATC AAGGAGGTAC AGCACGGGTG CATGGCCATC 
CACACCAAGG GACCACACAT GAACAAGCAA GGGGCATGCA CCAAGACCCT CGAAGAGACA 
GACTTGAATT CAGCCCTGTA TCCCTCATTC TCCCACTCGC CAAGTGCACC CAACCTGGAA 
TCTCCCAGCT GGCCCACCTC CACCAAAGTG TTGACATTCT GCAAGCCTAA GCAACCAGAG 
CCCAAGGCCT CACTGAAACG GATTTTGAGC CCCCTAGTTT TCGCATCTGG ATATAGGAAA 
GGAAGCAGAG AGAGATACCA GGATCAAAGG AAAGAGACAC CTTGCTCCAC CTGGTCATGG 
AAAAATGAAG ATTTGCACCC AAAATTCATG CAGTTGCTAC TTCTACACAC TCCTTACCAC 
AAAGACTATG AGTCCTTGAA CCGGGAAAGC TGGGATCACG GGGTGGTTGA GGGGCAAGGG 
CATTTGATTG AGGTCAGAGA CTTATTTGGC TCAGACCTGG GTCCCCAGGA AGGGCCTCAC 
ACAGTCATAT TGCATGGAGT TGCTGGAATT GGGAAGTCCA CACTGGCCAG GCACATAAGA 
AGAGCCTGGG AGGAGGGCCA GCTATACAGG AACCACTTCC AGCATGTCTT CTACTTCAAC 
TGCAGAGAGC TGGCCATGTC CAAGCTGACA AGTCTGGAGG AACTCATCAC AAAAGACCAG 
TCCACCACTG TGGCTCCCGC TGGGCAGGTC CAGTCTCAGC CGGAGCAGCT GCTCTTCATC 
CTCGATGGTT TAGATGAACT GGAATGGGTC TCAGAGGAGC AGAGGGCAGA GCTCTGTCTG 
CACCGGAGCC AGCAGCAGCC CGTGCAGACA CTGCTGGGCA GTTTGGTGGA GAAAACCATA 
TTTCCAGAGG CATCCTTGCT GATCACAGCT CGGACTGCAG CTCTGCAGAA GTTCATTCCT 
TCTTTGAAGC AGCCATGTTG GGTGGAGGTC CTAGGGTTCT CTGAGTCTTC CAGGAAGGAC 
TATTTCTATG AATATTTCAC AGAGGAAAGC CAAGCAACTA GAGCCTTTAA CGTGGTTGAA 
TCAAACCAAG CACTCTTGAC CATGTGTCTC ACGCCCTTGG TGTCCTGGCT GGCCTGCACT 
TGTCTGAAGC AGCAGATGGA GGCAGGGGAA GAACTCTCAC TGAGGTCACA GACCATGACA 
GCCCTCTATC TGCGCTACCT TGCCCAAGCT ATCCAAGCTC AGCCCCTGGG GACTCAGCTG 
AGGAGCTTCT GTACTCTTGC TGCTGAGGGT ATCTGGCAAG GAAAGACTCT GTTCACTCCA 
GGGGACCTCA AGAAGCACGG ATTAGATTGG GTCATCAACT CCACTCCCTT AAGGATGGAT 
GTTCTTCAAA AGCACCCCAC GTCTCTGAAC TACAGCTTCA TTCACCTGTG TTTCCAGGAG 
TTCTTTGCAG CAGTGTCCTT TGCCTTAGGG GACATGGGGG ACAAAAGTGA CCATCCTAAC 
AGCACTGGAA GCATGGAAAA GTTGGTAGAA GTATATGGGA TCAATAACCT GTTTGGGGCA 
CCAACCGTGC ATTTCCTATC CGGCCTTTTG AGTGAGCAAG GAGCGATAGA GATGGAGAAC 
ATTTTCCAAT GCAAGCTGTC TTGGGAGAGA AAGCGGGATT CACTGCTGCA GTGGGCTGAA 
TTGGAACTCG GGCGTGAGCA TTACTCTCTG CAATTGTTTC ACTGTTTGTA CGAGATTCAG 
GACGAGGAGT TCCTGACACA AGCGATGGCC CATTTCCAAG GAACAAGAGT GTGCATCCGA 
ACCAGCATGG AGCTGCTCGT GTTCACTTTC TGCATTAGAT TCTGCAGCCA CGTGCGGCAG 
CTTCAACTGA ATGAGGGCAG ACAACATCCA GGAGCGTGCA GGCCCACCGA CACAGTGCTG 
TTGAGCTGGG TCCCGGCCAC AGAGGCCTGC TGGCAGGTTC TCTGCTCCGT TCTCCAGGTC 
ACTGGAAACC TGAAGGAACT GGACCTGAGT GGAAACATCT TGAGCCGCTC TGCAGTACAG 
AGTCTTGATG AGGCCCTGAG ATGCCCTGGG TGTCAGCTAG AGACCCTGCG GCTGGCCAGC 
TGCGGCCTCA CAGCTGAGAG CTGCAAGGAC ATTGCCTCTG GGCTGAGCTC CAGCCAAACC 
CTGATGCAAT TGGAATTGAG CTTCAACACA CTCCTGGACG CAGGAGCTGA GCACCTTTGC 
CAGGGCCTGA GACAGCCAAC CTGCAAGCTG CAGCGACTGT TGCTGGCAGG CTGCGGCCTC 
ACGTCTGGCT GCTGCCAGGA CCTGGCCTCC ATGCTCAGGG TCAGCCCCAG CCTGATGGAG 
CTAAACCTGC TGCAGAATGA CCTGGACAAC CTCGGCGTGA GGCTGCTCTG TGAAGGACTC 
AGGCACCCTT CCTGCCAGCT CATATGCCTA TGGCTGGACC AGACTCAGCT GAGCGAGGAG 
GTGATGGAGA TGCTGAAGGC CCTGGAGAGG GAGAAGCCTC AGCTGCTAGT CTCCGGGATA 
TGGAAGCCAA GTCTGAGGAT CCTTCACGAG GGCCCAGGTG GAGCAGAGAT GAGCGATACA 
TCCTCCCTCA AGCGGCAGAG ACAAGCATCA GAGGGCAGCT CTCCTCAAGC AGCACAGGTG 
AGACTCTTCT GTCCGTCTTC TCCTGCCCCT CTGGAGGACA AGCACATGGA GTCTCTGGGG 
ACCGAGAATG ACCTCTGGGG CCCCACAGGG CCTGTGGCTA CCCAGGCAGT TGACGAAGAG 
AGGAGCCTGT ACCGCGTTCA CTTGCCCGTG GCCGGCTTCT ACCACTGGCC CAACACGGGT 
CTCCGCTTTG AGGTGAGAGG GCCAGTGACC GTTGAAATTG AATTCTGTGC CTGGGACCAG 
TTCCTGAACA GGAATTTCCC ACAGCACAGC TGGCTGGCTG CAGGGCCTCT GTTTGACATC 
AAGGCTGAAC CAGGCGCTGT GGCAGCCGTG TTCCTCCCTC ACTTTGTGGC CTTCCGAGGG 
AACGATGTGG ACATCTCCCA GTTTCAAGTG GCCCACTTCA AAGAGGAGGG GATGCTCCTG 
GAGAAGCCAG CCAGGGTGAC GCCATGTTAC GCTGTCCTGG AAAACCCCAG CTTCTCGCCC 
ATGGGAGTTC TACTGAGGAT GGTCCAAACT GCCCTGCGCT TCATTCCCAT CACCTCCACT 
GTGTTGCTCT ACCATTACCT CCAGCCTGAA GAAGTTACCT TCCACCTCTA CCTGATCCCC 
AGCGATTGCT CCATTCAGAA GGCCATAGAC AATGAAGAAA AGAAGTTCCA GTTTGTGCGA 
ATTCACAAGC CACCCCCGCT GACCCCACTC TACATGGGCT CCCGCTACAT TGTGTCTGGT 
TCAGAGAAGC TGGAAATACT GCCCGAGGAA TTGGAGCTCT GCTATCGGAG CCCCAGAGAA 
TCTCAGCTGT TCTCTGAGTT CTACGTTCGC CATTTGAGGT CAGGGATCAG GTTGCAAATA 
AGAAGCAAGA AAGACTGGGA CTGTGGTGTG GGAAGCCTTG GTGAAGCCAG GAGATCTCAG 
ACGGGCAGTT ACTCCAGTTC CTCCAGGCCT CAGAGCCTCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025136561.1
CDS	245..4009
Translation	MPALTRSPSPAAMAGEARRLEDLEEKKKEKLKELQLWLPDEKPCLQSPKVVYAQPEKLRIRRIMMIPSVIKEVQHGCMAIHTKGPHMNKQGACTKTLEETDLNSALYPSFSHSPSAPNLESPSWPTSTKVLTFCKPKQPEPKASLKRILSPLVFASGYRKGSRERYQDQRKETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGPQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELAMSKLTSLEELITKDQSTTVAPAGQVQSQPEQLLFILDGLDELEWVSEEQRAELCLHRSQQQPVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFNVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLRSQTMTALYLRYLAQAIQAQPLGTQLRSFCTLAAEGIWQGKTLFTPGDLKKHGLDWVINSTPLRMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDMGDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAIEMENIFQCKLSWERKRDSLLQWAELELGREHYSLQLFHCLYEIQDEEFLTQAMAHFQGTRVCIRTSMELLVFTFCIRFCSHVRQLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSGNILSRSAVQSLDEALRCPGCQLETLRLASCGLTAESCKDIASGLSSSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDNLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVMEMLKALEREKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPLEDKHMESLGTENDLWGPTGPVATQAVDEERSLYRVHLPVAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQHSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKKFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDWDCGVGSLGEARRSQTGSYSSSSRPQSLT

Target ORF information:

RefSeq Version	XM_025280776.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025280776.1

ORF Insert Sequence:

ATGCCTGCTC TGACAAGGTC TCCCAGCCCA GCAGCGATGG CCGGTGAAGC CCGGAGGCTG 
GAGGACTTGG AGGAAAAGAA GAAAGAGAAG CTGAAGGAGC TCCAGCTTTG GCTGCCTGAT 
GAGAAGCCCT GCTTGCAGTC TCCTAAGGTG GTCTATGCTC AACCAGAGAA GCTAAGGATC 
CGAAGGATCA TGATGATCCC CTCAGTGATC AAGGAGGTAC AGCACGGGTG CATGGCCATC 
CACACCAAGG GACCACACAT GAACAAGCAA GGGGCATGCA CCAAGACCCT CGAAGAGACA 
GACTTGAATT CAGCCCTGTA TCCCTCATTC TCCCACTCGC CAAGTGCACC CAACCTGGAA 
TCTCCCAGCT GGCCCACCTC CACCAAAGTG TTGACATTCT GCAAGCCTAA GCAACCAGAG 
CCCAAGGCCT CACTGAAACG GATTTTGAGC CCCCTAGTTT TCGCATCTGG ATATAGGAAA 
GGAAGCAGAG AGAGATACCA GGATCAAAGG AAAGAGACAC CTTGCTCCAC CTGGTCATGG 
AAAAATGAAG ATTTGCACCC AAAATTCATG CAGTTGCTAC TTCTACACAC TCCTTACCAC 
AAAGACTATG AGTCCTTGAA CCGGGAAAGC TGGGATCACG GGGTGGTTGA GGGGCAAGGG 
CATTTGATTG AGGTCAGAGA CTTATTTGGC TCAGACCTGG GTCCCCAGGA AGGGCCTCAC 
ACAGTCATAT TGCATGGAGT TGCTGGAATT GGGAAGTCCA CACTGGCCAG GCACATAAGA 
AGAGCCTGGG AGGAGGGCCA GCTATACAGG AACCACTTCC AGCATGTCTT CTACTTCAAC 
TGCAGAGAGC TGGCCATGTC CAAGCTGACA AGTCTGGAGG AACTCATCAC AAAAGACCAG 
TCCACCACTG TGGCTCCCGC TGGGCAGGTC CAGTCTCAGC CGGAGCAGCT GCTCTTCATC 
CTCGATGGTT TAGATGAACT GGAATGGGTC TCAGAGGAGC AGAGGGCAGA GCTCTGTCTG 
CACCGGAGCC AGCAGCAGCC CGTGCAGACA CTGCTGGGCA GTTTGGTGGA GAAAACCATA 
TTTCCAGAGG CATCCTTGCT GATCACAGCT CGGACTGCAG CTCTGCAGAA GTTCATTCCT 
TCTTTGAAGC AGCCATGTTG GGTGGAGGTC CTAGGGTTCT CTGAGTCTTC CAGGAAGGAC 
TATTTCTATG AATATTTCAC AGAGGAAAGC CAAGCAACTA GAGCCTTTAA CGTGGTTGAA 
TCAAACCAAG CACTCTTGAC CATGTGTCTC ACGCCCTTGG TGTCCTGGCT GGCCTGCACT 
TGTCTGAAGC AGCAGATGGA GGCAGGGGAA GAACTCTCAC TGAGGTCACA GACCATGACA 
GCCCTCTATC TGCGCTACCT TGCCCAAGCT ATCCAAGCTC AGCCCCTGGG GACTCAGCTG 
AGGAGCTTCT GTACTCTTGC TGCTGAGGGT ATCTGGCAAG GAAAGACTCT GTTCACTCCA 
GGGGACCTCA AGAAGCACGG ATTAGATTGG GTCATCAACT CCACTCCCTT AAGGATGGAT 
GTTCTTCAAA AGCACCCCAC GTCTCTGAAC TACAGCTTCA TTCACCTGTG TTTCCAGGAG 
TTCTTTGCAG CAGTGTCCTT TGCCTTAGGG GACATGGGGG ACAAAAGTGA CCATCCTAAC 
AGCACTGGAA GCATGGAAAA GTTGGTAGAA GTATATGGGA TCAATAACCT GTTTGGGGCA 
CCAACCGTGC ATTTCCTATC CGGCCTTTTG AGTGAGCAAG GAGCGATAGA GATGGAGAAC 
ATTTTCCAAT GCAAGCTGTC TTGGGAGAGA AAGCGGGATT CACTGCTGCA GTGGGCTGAA 
TTGGAACTCG GGCGTGAGCA TTACTCTCTG CAATTGTTTC ACTGTTTGTA CGAGATTCAG 
GACGAGGAGT TCCTGACACA AGCGATGGCC CATTTCCAAG GAACAAGAGT GTGCATCCGA 
ACCAGCATGG AGCTGCTCGT GTTCACTTTC TGCATTAGAT TCTGCAGCCA CGTGCGGCAG 
CTTCAACTGA ATGAGGGCAG ACAACATCCA GGAGCGTGCA GGCCCACCGA CACAGTGCTG 
TTGAGCTGGG TCCCGGCCAC AGAGGCCTGC TGGCAGGTTC TCTGCTCCGT TCTCCAGGTC 
ACTGGAAACC TGAAGGAACT GGACCTGAGT GGAAACATCT TGAGCCGCTC TGCAGTACAG 
AGTCTTGATG AGGCCCTGAG ATGCCCTGGG TGTCAGCTAG AGACCCTGCG GCTGGCCAGC 
TGCGGCCTCA CAGCTGAGAG CTGCAAGGAC ATTGCCTCTG GGCTGAGCTC CAGCCAAACC 
CTGATGCAAT TGGAATTGAG CTTCAACACA CTCCTGGACG CAGGAGCTGA GCACCTTTGC 
CAGGGCCTGA GACAGCCAAC CTGCAAGCTG CAGCGACTGT TGCTGGCAGG CTGCGGCCTC 
ACGTCTGGCT GCTGCCAGGA CCTGGCCTCC ATGCTCAGGG TCAGCCCCAG CCTGATGGAG 
CTAAACCTGC TGCAGAATGA CCTGGACAAC CTCGGCGTGA GGCTGCTCTG TGAAGGACTC 
AGGCACCCTT CCTGCCAGCT CATATGCCTA TGGCTGGACC AGACTCAGCT GAGCGAGGAG 
GTGATGGAGA TGCTGAAGGC CCTGGAGAGG GAGAAGCCTC AGCTGCTAGT CTCCGGGATA 
TGGAAGCCAA GTCTGAGGAT CCTTCACGAG GGCCCAGGTG GAGCAGAGAT GAGCGATACA 
TCCTCCCTCA AGCGGCAGAG ACAAGCATCA GAGGGCAGCT CTCCTCAAGC AGCACAGGTG 
AGACTCTTCT GTCCGTCTTC TCCTGCCCCT CTGGAGGACA AGCACATGGA GTCTCTGGGG 
ACCGAGAATG ACCTCTGGGG CCCCACAGGG CCTGTGGCTA CCCAGGCAGT TGACGAAGAG 
AGGAGCCTGT ACCGCGTTCA CTTGCCCGTG GCCGGCTTCT ACCACTGGCC CAACACGGGT 
CTCCGCTTTG AGGTGAGAGG GCCAGTGACC GTTGAAATTG AATTCTGTGC CTGGGACCAG 
TTCCTGAACA GGAATTTCCC ACAGCACAGC TGGCTGGCTG CAGGGCCTCT GTTTGACATC 
AAGGCTGAAC CAGGCGCTGT GGCAGCCGTG TTCCTCCCTC ACTTTGTGGC CTTCCGAGGG 
AACGATGTGG ACATCTCCCA GTTTCAAGTG GCCCACTTCA AAGAGGAGGG GATGCTCCTG 
GAGAAGCCAG CCAGGGTGAC GCCATGTTAC GCTGTCCTGG AAAACCCCAG CTTCTCGCCC 
ATGGGAGTTC TACTGAGGAT GGTCCAAACT GCCCTGCGCT TCATTCCCAT CACCTCCACT 
GTGTTGCTCT ACCATTACCT CCAGCCTGAA GAAGTTACCT TCCACCTCTA CCTGATCCCC 
AGCGATTGCT CCATTCAGAA GGCCATAGAC AATGAAGAAA AGAAGTTCCA GTTTGTGCGA 
ATTCACAAGC CACCCCCGCT GACCCCACTC TACATGGGCT CCCGCTACAT TGTGTCTGGT 
TCAGAGAAGC TGGAAATACT GCCCGAGGAA TTGGAGCTCT GCTATCGGAG CCCCAGAGAA 
TCTCAGCTGT TCTCTGAGTT CTACGTTCGC CATTTGAGGT CAGGGATCAG GTTGCAAATA 
AGAAGCAAGA AAGACTGGGA CTGTGGTGTG GGAAGCCTTG GTGAAGCCAG GAGATCTCAG 
ACGGGCAGTT ACTCCAGTTC CTCCAGGCCT CAGAGCCTCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_025280777.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025136562.1 Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X7, mRNA.

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CloneID	OBu62107
Clone ID Related Accession (Same CDS sequence)	XM_025280777.1
Accession Version	XM_025280777.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGCTC TGACAAGGTC TCCCAGCCCA GCAGCGATGG CCGGTGAAGC CCGGAGGCTG 
GAGGACTTGG AGGAAAAGAA GAAAGAGAAG CTGAAGGAGC TCCAGCTTTG GCTGCCTGAT 
GAGAAGCCCT GCTTGCAGTC TCCTAAGGTG GTCTATGCTC AACCAGAGAA GCTAAGGATC 
CGAAGGATCA TGATGATCCC CTCAGTGATC AAGGAGGTAC AGCACGGGTG CATGGCCATC 
CACACCAAGG GACCACACAT GAACAAGCAA GGGGCATGCA CCAAGACCCT CGAAGAGACA 
GACTTGAATT CAGGAAGCAG AGAGAGATAC CAGGATCAAA GGAAAGAGAC ACCTTGCTCC 
ACCTGGTCAT GGAAAAATGA AGATTTGCAC CCAAAATTCA TGCAGTTGCT ACTTCTACAC 
ACTCCTTACC ACAAAGACTA TGAGTCCTTG AACCGGGAAA GCTGGGATCA CGGGGTGGTT 
GAGGGGCAAG GGCATTTGAT TGAGGTCAGA GACTTATTTG GCTCAGACCT GGGTCCCCAG 
GAAGGGCCTC ACACAGTCAT ATTGCATGGA GTTGCTGGAA TTGGGAAGTC CACACTGGCC 
AGGCACATAA GAAGAGCCTG GGAGGAGGGC CAGCTATACA GGAACCACTT CCAGCATGTC 
TTCTACTTCA ACTGCAGAGA GCTGGCCATG TCCAAGCTGA CAAGTCTGGA GGAACTCATC 
ACAAAAGACC AGTCCACCAC TGTGGCTCCC GCTGGGCAGG TCCAGTCTCA GCCGGAGCAG 
CTGCTCTTCA TCCTCGATGG TTTAGATGAA CTGGAATGGG TCTCAGAGGA GCAGAGGGCA 
GAGCTCTGTC TGCACCGGAG CCAGCAGCAG CCCGTGCAGA CACTGCTGGG CAGTTTGGTG 
GAGAAAACCA TATTTCCAGA GGCATCCTTG CTGATCACAG CTCGGACTGC AGCTCTGCAG 
AAGTTCATTC CTTCTTTGAA GCAGCCATGT TGGGTGGAGG TCCTAGGGTT CTCTGAGTCT 
TCCAGGAAGG ACTATTTCTA TGAATATTTC ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT 
AACGTGGTTG AATCAAACCA AGCACTCTTG ACCATGTGTC TCACGCCCTT GGTGTCCTGG 
CTGGCCTGCA CTTGTCTGAA GCAGCAGATG GAGGCAGGGG AAGAACTCTC ACTGAGGTCA 
CAGACCATGA CAGCCCTCTA TCTGCGCTAC CTTGCCCAAG CTATCCAAGC TCAGCCCCTG 
GGGACTCAGC TGAGGAGCTT CTGTACTCTT GCTGCTGAGG GTATCTGGCA AGGAAAGACT 
CTGTTCACTC CAGGGGACCT CAAGAAGCAC GGATTAGATT GGGTCATCAA CTCCACTCCC 
TTAAGGATGG ATGTTCTTCA AAAGCACCCC ACGTCTCTGA ACTACAGCTT CATTCACCTG 
TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC TTTGCCTTAG GGGACATGGG GGACAAAAGT 
GACCATCCTA ACAGCACTGG AAGCATGGAA AAGTTGGTAG AAGTATATGG GATCAATAAC 
CTGTTTGGGG CACCAACCGT GCATTTCCTA TCCGGCCTTT TGAGTGAGCA AGGAGCGATA 
GAGATGGAGA ACATTTTCCA ATGCAAGCTG TCTTGGGAGA GAAAGCGGGA TTCACTGCTG 
CAGTGGGCTG AATTGGAACT CGGGCGTGAG CATTACTCTC TGCAATTGTT TCACTGTTTG 
TACGAGATTC AGGACGAGGA GTTCCTGACA CAAGCGATGG CCCATTTCCA AGGAACAAGA 
GTGTGCATCC GAACCAGCAT GGAGCTGCTC GTGTTCACTT TCTGCATTAG ATTCTGCAGC 
CACGTGCGGC AGCTTCAACT GAATGAGGGC AGACAACATC CAGGAGCGTG CAGGCCCACC 
GACACAGTGC TGTTGAGCTG GGTCCCGGCC ACAGAGGCCT GCTGGCAGGT TCTCTGCTCC 
GTTCTCCAGG TCACTGGAAA CCTGAAGGAA CTGGACCTGA GTGGAAACAT CTTGAGCCGC 
TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG AGATGCCCTG GGTGTCAGCT AGAGACCCTG 
CGGCTGGCCA GCTGCGGCCT CACAGCTGAG AGCTGCAAGG ACATTGCCTC TGGGCTGAGC 
TCCAGCCAAA CCCTGATGCA ATTGGAATTG AGCTTCAACA CACTCCTGGA CGCAGGAGCT 
GAGCACCTTT GCCAGGGCCT GAGACAGCCA ACCTGCAAGC TGCAGCGACT GTTGCTGGCA 
GGCTGCGGCC TCACGTCTGG CTGCTGCCAG GACCTGGCCT CCATGCTCAG GGTCAGCCCC 
AGCCTGATGG AGCTAAACCT GCTGCAGAAT GACCTGGACA ACCTCGGCGT GAGGCTGCTC 
TGTGAAGGAC TCAGGCACCC TTCCTGCCAG CTCATATGCC TATGGCTGGA CCAGACTCAG 
CTGAGCGAGG AGGTGATGGA GATGCTGAAG GCCCTGGAGA GGGAGAAGCC TCAGCTGCTA 
GTCTCCGGGA TATGGAAGCC AAGTCTGAGG ATCCTTCACG AGGGCCCAGG TGGAGCAGAG 
ATGAGCGATA CATCCTCCCT CAAGCGGCAG AGACAAGCAT CAGAGGGCAG CTCTCCTCAA 
GCAGCACAGG TGAGACTCTT CTGTCCGTCT TCTCCTGCCC CTCTGGAGGA CAAGCACATG 
GAGTCTCTGG GGACCGAGAA TGACCTCTGG GGCCCCACAG GGCCTGTGGC TACCCAGGCA 
GTTGACGAAG AGAGGAGCCT GTACCGCGTT CACTTGCCCG TGGCCGGCTT CTACCACTGG 
CCCAACACGG GTCTCCGCTT TGAGGTGAGA GGGCCAGTGA CCGTTGAAAT TGAATTCTGT 
GCCTGGGACC AGTTCCTGAA CAGGAATTTC CCACAGCACA GCTGGCTGGC TGCAGGGCCT 
CTGTTTGACA TCAAGGCTGA ACCAGGCGCT GTGGCAGCCG TGTTCCTCCC TCACTTTGTG 
GCCTTCCGAG GGAACGATGT GGACATCTCC CAGTTTCAAG TGGCCCACTT CAAAGAGGAG 
GGGATGCTCC TGGAGAAGCC AGCCAGGGTG ACGCCATGTT ACGCTGTCCT GGAAAACCCC 
AGCTTCTCGC CCATGGGAGT TCTACTGAGG ATGGTCCAAA CTGCCCTGCG CTTCATTCCC 
ATCACCTCCA CTGTGTTGCT CTACCATTAC CTCCAGCCTG AAGAAGTTAC CTTCCACCTC 
TACCTGATCC CCAGCGATTG CTCCATTCAG AAGGCCATAG ACAATGAAGA AAAGAAGTTC 
CAGTTTGTGC GAATTCACAA GCCACCCCCG CTGACCCCAC TCTACATGGG CTCCCGCTAC 
ATTGTGTCTG GTTCAGAGAA GCTGGAAATA CTGCCCGAGG AATTGGAGCT CTGCTATCGG 
AGCCCCAGAG AATCTCAGCT GTTCTCTGAG TTCTACGTTC GCCATTTGAG GTCAGGGATC 
AGGTTGCAAA TAAGAAGCAA GAAAGACTGG GACTGTGGTG TGGGAAGCCT TGGTGAAGCC 
AGGAGATCTC AGACGGGCAG TTACTCCAGT TCCTCCAGGC CTCAGAGCCT CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025136562.1
CDS	245..3841
Translation	MPALTRSPSPAAMAGEARRLEDLEEKKKEKLKELQLWLPDEKPCLQSPKVVYAQPEKLRIRRIMMIPSVIKEVQHGCMAIHTKGPHMNKQGACTKTLEETDLNSGSRERYQDQRKETPCSTWSWKNEDLHPKFMQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGPQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELAMSKLTSLEELITKDQSTTVAPAGQVQSQPEQLLFILDGLDELEWVSEEQRAELCLHRSQQQPVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFNVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLRSQTMTALYLRYLAQAIQAQPLGTQLRSFCTLAAEGIWQGKTLFTPGDLKKHGLDWVINSTPLRMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDMGDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAIEMENIFQCKLSWERKRDSLLQWAELELGREHYSLQLFHCLYEIQDEEFLTQAMAHFQGTRVCIRTSMELLVFTFCIRFCSHVRQLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSGNILSRSAVQSLDEALRCPGCQLETLRLASCGLTAESCKDIASGLSSSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDNLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVMEMLKALEREKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPLEDKHMESLGTENDLWGPTGPVATQAVDEERSLYRVHLPVAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQHSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKKFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDWDCGVGSLGEARRSQTGSYSSSSRPQSLT

Target ORF information:

RefSeq Version	XM_025280777.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025280777.1

ORF Insert Sequence:

ATGCCTGCTC TGACAAGGTC TCCCAGCCCA GCAGCGATGG CCGGTGAAGC CCGGAGGCTG 
GAGGACTTGG AGGAAAAGAA GAAAGAGAAG CTGAAGGAGC TCCAGCTTTG GCTGCCTGAT 
GAGAAGCCCT GCTTGCAGTC TCCTAAGGTG GTCTATGCTC AACCAGAGAA GCTAAGGATC 
CGAAGGATCA TGATGATCCC CTCAGTGATC AAGGAGGTAC AGCACGGGTG CATGGCCATC 
CACACCAAGG GACCACACAT GAACAAGCAA GGGGCATGCA CCAAGACCCT CGAAGAGACA 
GACTTGAATT CAGGAAGCAG AGAGAGATAC CAGGATCAAA GGAAAGAGAC ACCTTGCTCC 
ACCTGGTCAT GGAAAAATGA AGATTTGCAC CCAAAATTCA TGCAGTTGCT ACTTCTACAC 
ACTCCTTACC ACAAAGACTA TGAGTCCTTG AACCGGGAAA GCTGGGATCA CGGGGTGGTT 
GAGGGGCAAG GGCATTTGAT TGAGGTCAGA GACTTATTTG GCTCAGACCT GGGTCCCCAG 
GAAGGGCCTC ACACAGTCAT ATTGCATGGA GTTGCTGGAA TTGGGAAGTC CACACTGGCC 
AGGCACATAA GAAGAGCCTG GGAGGAGGGC CAGCTATACA GGAACCACTT CCAGCATGTC 
TTCTACTTCA ACTGCAGAGA GCTGGCCATG TCCAAGCTGA CAAGTCTGGA GGAACTCATC 
ACAAAAGACC AGTCCACCAC TGTGGCTCCC GCTGGGCAGG TCCAGTCTCA GCCGGAGCAG 
CTGCTCTTCA TCCTCGATGG TTTAGATGAA CTGGAATGGG TCTCAGAGGA GCAGAGGGCA 
GAGCTCTGTC TGCACCGGAG CCAGCAGCAG CCCGTGCAGA CACTGCTGGG CAGTTTGGTG 
GAGAAAACCA TATTTCCAGA GGCATCCTTG CTGATCACAG CTCGGACTGC AGCTCTGCAG 
AAGTTCATTC CTTCTTTGAA GCAGCCATGT TGGGTGGAGG TCCTAGGGTT CTCTGAGTCT 
TCCAGGAAGG ACTATTTCTA TGAATATTTC ACAGAGGAAA GCCAAGCAAC TAGAGCCTTT 
AACGTGGTTG AATCAAACCA AGCACTCTTG ACCATGTGTC TCACGCCCTT GGTGTCCTGG 
CTGGCCTGCA CTTGTCTGAA GCAGCAGATG GAGGCAGGGG AAGAACTCTC ACTGAGGTCA 
CAGACCATGA CAGCCCTCTA TCTGCGCTAC CTTGCCCAAG CTATCCAAGC TCAGCCCCTG 
GGGACTCAGC TGAGGAGCTT CTGTACTCTT GCTGCTGAGG GTATCTGGCA AGGAAAGACT 
CTGTTCACTC CAGGGGACCT CAAGAAGCAC GGATTAGATT GGGTCATCAA CTCCACTCCC 
TTAAGGATGG ATGTTCTTCA AAAGCACCCC ACGTCTCTGA ACTACAGCTT CATTCACCTG 
TGTTTCCAGG AGTTCTTTGC AGCAGTGTCC TTTGCCTTAG GGGACATGGG GGACAAAAGT 
GACCATCCTA ACAGCACTGG AAGCATGGAA AAGTTGGTAG AAGTATATGG GATCAATAAC 
CTGTTTGGGG CACCAACCGT GCATTTCCTA TCCGGCCTTT TGAGTGAGCA AGGAGCGATA 
GAGATGGAGA ACATTTTCCA ATGCAAGCTG TCTTGGGAGA GAAAGCGGGA TTCACTGCTG 
CAGTGGGCTG AATTGGAACT CGGGCGTGAG CATTACTCTC TGCAATTGTT TCACTGTTTG 
TACGAGATTC AGGACGAGGA GTTCCTGACA CAAGCGATGG CCCATTTCCA AGGAACAAGA 
GTGTGCATCC GAACCAGCAT GGAGCTGCTC GTGTTCACTT TCTGCATTAG ATTCTGCAGC 
CACGTGCGGC AGCTTCAACT GAATGAGGGC AGACAACATC CAGGAGCGTG CAGGCCCACC 
GACACAGTGC TGTTGAGCTG GGTCCCGGCC ACAGAGGCCT GCTGGCAGGT TCTCTGCTCC 
GTTCTCCAGG TCACTGGAAA CCTGAAGGAA CTGGACCTGA GTGGAAACAT CTTGAGCCGC 
TCTGCAGTAC AGAGTCTTGA TGAGGCCCTG AGATGCCCTG GGTGTCAGCT AGAGACCCTG 
CGGCTGGCCA GCTGCGGCCT CACAGCTGAG AGCTGCAAGG ACATTGCCTC TGGGCTGAGC 
TCCAGCCAAA CCCTGATGCA ATTGGAATTG AGCTTCAACA CACTCCTGGA CGCAGGAGCT 
GAGCACCTTT GCCAGGGCCT GAGACAGCCA ACCTGCAAGC TGCAGCGACT GTTGCTGGCA 
GGCTGCGGCC TCACGTCTGG CTGCTGCCAG GACCTGGCCT CCATGCTCAG GGTCAGCCCC 
AGCCTGATGG AGCTAAACCT GCTGCAGAAT GACCTGGACA ACCTCGGCGT GAGGCTGCTC 
TGTGAAGGAC TCAGGCACCC TTCCTGCCAG CTCATATGCC TATGGCTGGA CCAGACTCAG 
CTGAGCGAGG AGGTGATGGA GATGCTGAAG GCCCTGGAGA GGGAGAAGCC TCAGCTGCTA 
GTCTCCGGGA TATGGAAGCC AAGTCTGAGG ATCCTTCACG AGGGCCCAGG TGGAGCAGAG 
ATGAGCGATA CATCCTCCCT CAAGCGGCAG AGACAAGCAT CAGAGGGCAG CTCTCCTCAA 
GCAGCACAGG TGAGACTCTT CTGTCCGTCT TCTCCTGCCC CTCTGGAGGA CAAGCACATG 
GAGTCTCTGG GGACCGAGAA TGACCTCTGG GGCCCCACAG GGCCTGTGGC TACCCAGGCA 
GTTGACGAAG AGAGGAGCCT GTACCGCGTT CACTTGCCCG TGGCCGGCTT CTACCACTGG 
CCCAACACGG GTCTCCGCTT TGAGGTGAGA GGGCCAGTGA CCGTTGAAAT TGAATTCTGT 
GCCTGGGACC AGTTCCTGAA CAGGAATTTC CCACAGCACA GCTGGCTGGC TGCAGGGCCT 
CTGTTTGACA TCAAGGCTGA ACCAGGCGCT GTGGCAGCCG TGTTCCTCCC TCACTTTGTG 
GCCTTCCGAG GGAACGATGT GGACATCTCC CAGTTTCAAG TGGCCCACTT CAAAGAGGAG 
GGGATGCTCC TGGAGAAGCC AGCCAGGGTG ACGCCATGTT ACGCTGTCCT GGAAAACCCC 
AGCTTCTCGC CCATGGGAGT TCTACTGAGG ATGGTCCAAA CTGCCCTGCG CTTCATTCCC 
ATCACCTCCA CTGTGTTGCT CTACCATTAC CTCCAGCCTG AAGAAGTTAC CTTCCACCTC 
TACCTGATCC CCAGCGATTG CTCCATTCAG AAGGCCATAG ACAATGAAGA AAAGAAGTTC 
CAGTTTGTGC GAATTCACAA GCCACCCCCG CTGACCCCAC TCTACATGGG CTCCCGCTAC 
ATTGTGTCTG GTTCAGAGAA GCTGGAAATA CTGCCCGAGG AATTGGAGCT CTGCTATCGG 
AGCCCCAGAG AATCTCAGCT GTTCTCTGAG TTCTACGTTC GCCATTTGAG GTCAGGGATC 
AGGTTGCAAA TAAGAAGCAA GAAAGACTGG GACTGTGGTG TGGGAAGCCT TGGTGAAGCC 
AGGAGATCTC AGACGGGCAG TTACTCCAGT TCCTCCAGGC CTCAGAGCCT CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_025280778.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025136563.1 Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBu62108
Clone ID Related Accession (Same CDS sequence)	XM_025280778.1 , XM_006060029.2 , XM_006060028.2 , XM_025280781.1 , XM_025280779.1 , XM_025280780.1
Accession Version	XM_025280778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ATGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTCCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAT GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGGCAG 
GTCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGG 
GTCTCAGAGG AGCAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GCCCGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTAGGGT TCTCTGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAACGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGT 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGTCTGA AGCAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAGGTC ACAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGGACTCAG CTGAGGAGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGGC AAGGAAAGAC TCTGTTCACT CCAGGGGACC TCAAGAAGCA CGGATTAGAT 
TGGGTCATCA ACTCCACTCC CTTAAGGATG GATGTTCTTC AAAAGCACCC CACGTCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATGG GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTGTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAT AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG ATTCACTGCT GCAGTGGGCT GAATTGGAAC TCGGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAACAAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGCGG CAGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCGT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAGGCC 
TGCTGGCAGG TTCTCTGCTC CGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTGGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGTCAGC TAGAGACCCT GCGGCTGGCC AGCTGCGGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CTCCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
AACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGATGG AGATGCTGAA GGCCCTGGAG 
AGGGAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGCGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGACTCT TCTGTCCGTC TTCTCCTGCC 
CCTCTGGAGG ACAAGCACAT GGAGTCTCTG GGGACCGAGA ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCCAGGC AGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
GTGGCCGGCT TCTACCACTG GCCCAACACG GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCAC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCTGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGAAGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGACTG GGACTGTGGT 
GTGGGAAGCC TTGGTGAAGC CAGGAGATCT CAGACGGGCA GTTACTCCAG TTCCTCCAGG 
CCTCAGAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025136563.1
CDS	725..3922
Translation	MQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGPQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELAMSKLTSLEELITKDQSTTVAPAGQVQSQPEQLLFILDGLDELEWVSEEQRAELCLHRSQQQPVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFNVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLRSQTMTALYLRYLAQAIQAQPLGTQLRSFCTLAAEGIWQGKTLFTPGDLKKHGLDWVINSTPLRMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDMGDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAIEMENIFQCKLSWERKRDSLLQWAELELGREHYSLQLFHCLYEIQDEEFLTQAMAHFQGTRVCIRTSMELLVFTFCIRFCSHVRQLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSGNILSRSAVQSLDEALRCPGCQLETLRLASCGLTAESCKDIASGLSSSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDNLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVMEMLKALEREKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPLEDKHMESLGTENDLWGPTGPVATQAVDEERSLYRVHLPVAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQHSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKKFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDWDCGVGSLGEARRSQTGSYSSSSRPQSLT

Target ORF information:

RefSeq Version	XM_025280778.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025280778.1

ORF Insert Sequence:

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ATGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTCCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAT GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGGCAG 
GTCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGG 
GTCTCAGAGG AGCAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GCCCGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTAGGGT TCTCTGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAACGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGT 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGTCTGA AGCAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAGGTC ACAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGGACTCAG CTGAGGAGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGGC AAGGAAAGAC TCTGTTCACT CCAGGGGACC TCAAGAAGCA CGGATTAGAT 
TGGGTCATCA ACTCCACTCC CTTAAGGATG GATGTTCTTC AAAAGCACCC CACGTCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATGG GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTGTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAT AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG ATTCACTGCT GCAGTGGGCT GAATTGGAAC TCGGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAACAAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGCGG CAGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCGT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAGGCC 
TGCTGGCAGG TTCTCTGCTC CGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTGGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGTCAGC TAGAGACCCT GCGGCTGGCC AGCTGCGGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CTCCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
AACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGATGG AGATGCTGAA GGCCCTGGAG 
AGGGAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGCGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGACTCT TCTGTCCGTC TTCTCCTGCC 
CCTCTGGAGG ACAAGCACAT GGAGTCTCTG GGGACCGAGA ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCCAGGC AGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
GTGGCCGGCT TCTACCACTG GCCCAACACG GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCAC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCTGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGAAGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGACTG GGACTGTGGT 
GTGGGAAGCC TTGGTGAAGC CAGGAGATCT CAGACGGGCA GTTACTCCAG TTCCTCCAGG 
CCTCAGAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_006060029.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006060091.2 Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBu62108
Clone ID Related Accession (Same CDS sequence)	XM_025280778.1 , XM_006060029.2 , XM_006060028.2 , XM_025280781.1 , XM_025280779.1 , XM_025280780.1
Accession Version	XM_006060029.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006060029.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ATGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTCCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAT GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGGCAG 
GTCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGG 
GTCTCAGAGG AGCAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GCCCGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTAGGGT TCTCTGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAACGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGT 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGTCTGA AGCAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAGGTC ACAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGGACTCAG CTGAGGAGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGGC AAGGAAAGAC TCTGTTCACT CCAGGGGACC TCAAGAAGCA CGGATTAGAT 
TGGGTCATCA ACTCCACTCC CTTAAGGATG GATGTTCTTC AAAAGCACCC CACGTCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATGG GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTGTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAT AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG ATTCACTGCT GCAGTGGGCT GAATTGGAAC TCGGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAACAAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGCGG CAGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCGT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAGGCC 
TGCTGGCAGG TTCTCTGCTC CGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTGGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGTCAGC TAGAGACCCT GCGGCTGGCC AGCTGCGGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CTCCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
AACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGATGG AGATGCTGAA GGCCCTGGAG 
AGGGAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGCGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGACTCT TCTGTCCGTC TTCTCCTGCC 
CCTCTGGAGG ACAAGCACAT GGAGTCTCTG GGGACCGAGA ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCCAGGC AGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
GTGGCCGGCT TCTACCACTG GCCCAACACG GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCAC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCTGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGAAGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGACTG GGACTGTGGT 
GTGGGAAGCC TTGGTGAAGC CAGGAGATCT CAGACGGGCA GTTACTCCAG TTCCTCCAGG 
CCTCAGAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006060091.2
CDS	525..3722
Translation	MQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGPQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELAMSKLTSLEELITKDQSTTVAPAGQVQSQPEQLLFILDGLDELEWVSEEQRAELCLHRSQQQPVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFNVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLRSQTMTALYLRYLAQAIQAQPLGTQLRSFCTLAAEGIWQGKTLFTPGDLKKHGLDWVINSTPLRMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDMGDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAIEMENIFQCKLSWERKRDSLLQWAELELGREHYSLQLFHCLYEIQDEEFLTQAMAHFQGTRVCIRTSMELLVFTFCIRFCSHVRQLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSGNILSRSAVQSLDEALRCPGCQLETLRLASCGLTAESCKDIASGLSSSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDNLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVMEMLKALEREKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPLEDKHMESLGTENDLWGPTGPVATQAVDEERSLYRVHLPVAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQHSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKKFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDWDCGVGSLGEARRSQTGSYSSSSRPQSLT

Target ORF information:

RefSeq Version	XM_006060029.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006060029.2

ORF Insert Sequence:

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ATGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTCCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAT GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGGCAG 
GTCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGG 
GTCTCAGAGG AGCAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GCCCGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTAGGGT TCTCTGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAACGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGT 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGTCTGA AGCAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAGGTC ACAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGGACTCAG CTGAGGAGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGGC AAGGAAAGAC TCTGTTCACT CCAGGGGACC TCAAGAAGCA CGGATTAGAT 
TGGGTCATCA ACTCCACTCC CTTAAGGATG GATGTTCTTC AAAAGCACCC CACGTCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATGG GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTGTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAT AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG ATTCACTGCT GCAGTGGGCT GAATTGGAAC TCGGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAACAAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGCGG CAGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCGT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAGGCC 
TGCTGGCAGG TTCTCTGCTC CGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTGGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGTCAGC TAGAGACCCT GCGGCTGGCC AGCTGCGGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CTCCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
AACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGATGG AGATGCTGAA GGCCCTGGAG 
AGGGAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGCGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGACTCT TCTGTCCGTC TTCTCCTGCC 
CCTCTGGAGG ACAAGCACAT GGAGTCTCTG GGGACCGAGA ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCCAGGC AGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
GTGGCCGGCT TCTACCACTG GCCCAACACG GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCAC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCTGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGAAGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGACTG GGACTGTGGT 
GTGGGAAGCC TTGGTGAAGC CAGGAGATCT CAGACGGGCA GTTACTCCAG TTCCTCCAGG 
CCTCAGAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_006060028.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006060090.2 Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBu62108
Clone ID Related Accession (Same CDS sequence)	XM_025280778.1 , XM_006060029.2 , XM_006060028.2 , XM_025280781.1 , XM_025280779.1 , XM_025280780.1
Accession Version	XM_006060028.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006060028.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ATGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTCCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAT GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGGCAG 
GTCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGG 
GTCTCAGAGG AGCAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GCCCGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTAGGGT TCTCTGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAACGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGT 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGTCTGA AGCAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAGGTC ACAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGGACTCAG CTGAGGAGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGGC AAGGAAAGAC TCTGTTCACT CCAGGGGACC TCAAGAAGCA CGGATTAGAT 
TGGGTCATCA ACTCCACTCC CTTAAGGATG GATGTTCTTC AAAAGCACCC CACGTCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATGG GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTGTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAT AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG ATTCACTGCT GCAGTGGGCT GAATTGGAAC TCGGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAACAAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGCGG CAGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCGT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAGGCC 
TGCTGGCAGG TTCTCTGCTC CGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTGGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGTCAGC TAGAGACCCT GCGGCTGGCC AGCTGCGGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CTCCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
AACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGATGG AGATGCTGAA GGCCCTGGAG 
AGGGAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGCGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGACTCT TCTGTCCGTC TTCTCCTGCC 
CCTCTGGAGG ACAAGCACAT GGAGTCTCTG GGGACCGAGA ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCCAGGC AGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
GTGGCCGGCT TCTACCACTG GCCCAACACG GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCAC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCTGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGAAGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGACTG GGACTGTGGT 
GTGGGAAGCC TTGGTGAAGC CAGGAGATCT CAGACGGGCA GTTACTCCAG TTCCTCCAGG 
CCTCAGAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006060090.2
CDS	347..3544
Translation	MQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGPQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELAMSKLTSLEELITKDQSTTVAPAGQVQSQPEQLLFILDGLDELEWVSEEQRAELCLHRSQQQPVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFNVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLRSQTMTALYLRYLAQAIQAQPLGTQLRSFCTLAAEGIWQGKTLFTPGDLKKHGLDWVINSTPLRMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDMGDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAIEMENIFQCKLSWERKRDSLLQWAELELGREHYSLQLFHCLYEIQDEEFLTQAMAHFQGTRVCIRTSMELLVFTFCIRFCSHVRQLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSGNILSRSAVQSLDEALRCPGCQLETLRLASCGLTAESCKDIASGLSSSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDNLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVMEMLKALEREKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPLEDKHMESLGTENDLWGPTGPVATQAVDEERSLYRVHLPVAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQHSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKKFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDWDCGVGSLGEARRSQTGSYSSSSRPQSLT

Target ORF information:

RefSeq Version	XM_006060028.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006060028.2

ORF Insert Sequence:

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ATGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTCCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAT GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGGCAG 
GTCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGG 
GTCTCAGAGG AGCAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GCCCGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTAGGGT TCTCTGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAACGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGT 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGTCTGA AGCAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAGGTC ACAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGGACTCAG CTGAGGAGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGGC AAGGAAAGAC TCTGTTCACT CCAGGGGACC TCAAGAAGCA CGGATTAGAT 
TGGGTCATCA ACTCCACTCC CTTAAGGATG GATGTTCTTC AAAAGCACCC CACGTCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATGG GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTGTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAT AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG ATTCACTGCT GCAGTGGGCT GAATTGGAAC TCGGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAACAAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGCGG CAGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCGT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAGGCC 
TGCTGGCAGG TTCTCTGCTC CGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTGGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGTCAGC TAGAGACCCT GCGGCTGGCC AGCTGCGGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CTCCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
AACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGATGG AGATGCTGAA GGCCCTGGAG 
AGGGAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGCGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGACTCT TCTGTCCGTC TTCTCCTGCC 
CCTCTGGAGG ACAAGCACAT GGAGTCTCTG GGGACCGAGA ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCCAGGC AGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
GTGGCCGGCT TCTACCACTG GCCCAACACG GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCAC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCTGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGAAGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGACTG GGACTGTGGT 
GTGGGAAGCC TTGGTGAAGC CAGGAGATCT CAGACGGGCA GTTACTCCAG TTCCTCCAGG 
CCTCAGAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_025280781.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025136566.1 Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBu62108
Clone ID Related Accession (Same CDS sequence)	XM_025280778.1 , XM_006060029.2 , XM_006060028.2 , XM_025280781.1 , XM_025280779.1 , XM_025280780.1
Accession Version	XM_025280781.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ATGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTCCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAT GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGGCAG 
GTCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGG 
GTCTCAGAGG AGCAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GCCCGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTAGGGT TCTCTGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAACGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGT 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGTCTGA AGCAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAGGTC ACAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGGACTCAG CTGAGGAGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGGC AAGGAAAGAC TCTGTTCACT CCAGGGGACC TCAAGAAGCA CGGATTAGAT 
TGGGTCATCA ACTCCACTCC CTTAAGGATG GATGTTCTTC AAAAGCACCC CACGTCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATGG GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTGTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAT AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG ATTCACTGCT GCAGTGGGCT GAATTGGAAC TCGGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAACAAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGCGG CAGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCGT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAGGCC 
TGCTGGCAGG TTCTCTGCTC CGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTGGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGTCAGC TAGAGACCCT GCGGCTGGCC AGCTGCGGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CTCCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
AACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGATGG AGATGCTGAA GGCCCTGGAG 
AGGGAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGCGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGACTCT TCTGTCCGTC TTCTCCTGCC 
CCTCTGGAGG ACAAGCACAT GGAGTCTCTG GGGACCGAGA ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCCAGGC AGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
GTGGCCGGCT TCTACCACTG GCCCAACACG GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCAC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCTGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGAAGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGACTG GGACTGTGGT 
GTGGGAAGCC TTGGTGAAGC CAGGAGATCT CAGACGGGCA GTTACTCCAG TTCCTCCAGG 
CCTCAGAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025136566.1
CDS	385..3582
Translation	MQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGPQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELAMSKLTSLEELITKDQSTTVAPAGQVQSQPEQLLFILDGLDELEWVSEEQRAELCLHRSQQQPVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFNVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLRSQTMTALYLRYLAQAIQAQPLGTQLRSFCTLAAEGIWQGKTLFTPGDLKKHGLDWVINSTPLRMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDMGDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAIEMENIFQCKLSWERKRDSLLQWAELELGREHYSLQLFHCLYEIQDEEFLTQAMAHFQGTRVCIRTSMELLVFTFCIRFCSHVRQLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSGNILSRSAVQSLDEALRCPGCQLETLRLASCGLTAESCKDIASGLSSSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDNLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVMEMLKALEREKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPLEDKHMESLGTENDLWGPTGPVATQAVDEERSLYRVHLPVAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQHSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKKFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDWDCGVGSLGEARRSQTGSYSSSSRPQSLT

Target ORF information:

RefSeq Version	XM_025280781.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025280781.1

ORF Insert Sequence:

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ATGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTCCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAT GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGGCAG 
GTCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGG 
GTCTCAGAGG AGCAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GCCCGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTAGGGT TCTCTGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAACGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGT 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGTCTGA AGCAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAGGTC ACAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGGACTCAG CTGAGGAGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGGC AAGGAAAGAC TCTGTTCACT CCAGGGGACC TCAAGAAGCA CGGATTAGAT 
TGGGTCATCA ACTCCACTCC CTTAAGGATG GATGTTCTTC AAAAGCACCC CACGTCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATGG GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTGTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAT AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG ATTCACTGCT GCAGTGGGCT GAATTGGAAC TCGGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAACAAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGCGG CAGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCGT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAGGCC 
TGCTGGCAGG TTCTCTGCTC CGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTGGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGTCAGC TAGAGACCCT GCGGCTGGCC AGCTGCGGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CTCCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
AACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGATGG AGATGCTGAA GGCCCTGGAG 
AGGGAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGCGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGACTCT TCTGTCCGTC TTCTCCTGCC 
CCTCTGGAGG ACAAGCACAT GGAGTCTCTG GGGACCGAGA ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCCAGGC AGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
GTGGCCGGCT TCTACCACTG GCCCAACACG GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCAC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCTGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGAAGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGACTG GGACTGTGGT 
GTGGGAAGCC TTGGTGAAGC CAGGAGATCT CAGACGGGCA GTTACTCCAG TTCCTCCAGG 
CCTCAGAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_025280779.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025136564.1 Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBu62108
Clone ID Related Accession (Same CDS sequence)	XM_025280778.1 , XM_006060029.2 , XM_006060028.2 , XM_025280781.1 , XM_025280779.1 , XM_025280780.1
Accession Version	XM_025280779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ATGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTCCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAT GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGGCAG 
GTCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGG 
GTCTCAGAGG AGCAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GCCCGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTAGGGT TCTCTGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAACGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGT 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGTCTGA AGCAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAGGTC ACAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGGACTCAG CTGAGGAGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGGC AAGGAAAGAC TCTGTTCACT CCAGGGGACC TCAAGAAGCA CGGATTAGAT 
TGGGTCATCA ACTCCACTCC CTTAAGGATG GATGTTCTTC AAAAGCACCC CACGTCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATGG GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTGTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAT AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG ATTCACTGCT GCAGTGGGCT GAATTGGAAC TCGGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAACAAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGCGG CAGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCGT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAGGCC 
TGCTGGCAGG TTCTCTGCTC CGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTGGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGTCAGC TAGAGACCCT GCGGCTGGCC AGCTGCGGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CTCCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
AACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGATGG AGATGCTGAA GGCCCTGGAG 
AGGGAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGCGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGACTCT TCTGTCCGTC TTCTCCTGCC 
CCTCTGGAGG ACAAGCACAT GGAGTCTCTG GGGACCGAGA ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCCAGGC AGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
GTGGCCGGCT TCTACCACTG GCCCAACACG GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCAC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCTGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGAAGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGACTG GGACTGTGGT 
GTGGGAAGCC TTGGTGAAGC CAGGAGATCT CAGACGGGCA GTTACTCCAG TTCCTCCAGG 
CCTCAGAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025136564.1
CDS	547..3744
Translation	MQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGPQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELAMSKLTSLEELITKDQSTTVAPAGQVQSQPEQLLFILDGLDELEWVSEEQRAELCLHRSQQQPVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFNVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLRSQTMTALYLRYLAQAIQAQPLGTQLRSFCTLAAEGIWQGKTLFTPGDLKKHGLDWVINSTPLRMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDMGDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAIEMENIFQCKLSWERKRDSLLQWAELELGREHYSLQLFHCLYEIQDEEFLTQAMAHFQGTRVCIRTSMELLVFTFCIRFCSHVRQLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSGNILSRSAVQSLDEALRCPGCQLETLRLASCGLTAESCKDIASGLSSSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDNLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVMEMLKALEREKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPLEDKHMESLGTENDLWGPTGPVATQAVDEERSLYRVHLPVAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQHSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKKFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDWDCGVGSLGEARRSQTGSYSSSSRPQSLT

Target ORF information:

RefSeq Version	XM_025280779.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025280779.1

ORF Insert Sequence:

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ATGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTCCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAT GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGGCAG 
GTCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGG 
GTCTCAGAGG AGCAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GCCCGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTAGGGT TCTCTGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAACGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGT 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGTCTGA AGCAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAGGTC ACAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGGACTCAG CTGAGGAGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGGC AAGGAAAGAC TCTGTTCACT CCAGGGGACC TCAAGAAGCA CGGATTAGAT 
TGGGTCATCA ACTCCACTCC CTTAAGGATG GATGTTCTTC AAAAGCACCC CACGTCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATGG GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTGTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAT AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG ATTCACTGCT GCAGTGGGCT GAATTGGAAC TCGGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAACAAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGCGG CAGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCGT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAGGCC 
TGCTGGCAGG TTCTCTGCTC CGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTGGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGTCAGC TAGAGACCCT GCGGCTGGCC AGCTGCGGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CTCCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
AACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGATGG AGATGCTGAA GGCCCTGGAG 
AGGGAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGCGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGACTCT TCTGTCCGTC TTCTCCTGCC 
CCTCTGGAGG ACAAGCACAT GGAGTCTCTG GGGACCGAGA ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCCAGGC AGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
GTGGCCGGCT TCTACCACTG GCCCAACACG GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCAC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCTGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGAAGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGACTG GGACTGTGGT 
GTGGGAAGCC TTGGTGAAGC CAGGAGATCT CAGACGGGCA GTTACTCCAG TTCCTCCAGG 
CCTCAGAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP1 ( XM_025280780.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025136565.1 Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBu62108
Clone ID Related Accession (Same CDS sequence)	XM_025280778.1 , XM_006060029.2 , XM_006060028.2 , XM_025280781.1 , XM_025280779.1 , XM_025280780.1
Accession Version	XM_025280780.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	NACHT, LRR and PYD domains-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ATGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTCCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAT GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGGCAG 
GTCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGG 
GTCTCAGAGG AGCAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GCCCGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTAGGGT TCTCTGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAACGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGT 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGTCTGA AGCAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAGGTC ACAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGGACTCAG CTGAGGAGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGGC AAGGAAAGAC TCTGTTCACT CCAGGGGACC TCAAGAAGCA CGGATTAGAT 
TGGGTCATCA ACTCCACTCC CTTAAGGATG GATGTTCTTC AAAAGCACCC CACGTCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATGG GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTGTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAT AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG ATTCACTGCT GCAGTGGGCT GAATTGGAAC TCGGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAACAAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGCGG CAGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCGT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAGGCC 
TGCTGGCAGG TTCTCTGCTC CGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTGGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGTCAGC TAGAGACCCT GCGGCTGGCC AGCTGCGGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CTCCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
AACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGATGG AGATGCTGAA GGCCCTGGAG 
AGGGAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGCGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGACTCT TCTGTCCGTC TTCTCCTGCC 
CCTCTGGAGG ACAAGCACAT GGAGTCTCTG GGGACCGAGA ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCCAGGC AGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
GTGGCCGGCT TCTACCACTG GCCCAACACG GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCAC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCTGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGAAGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGACTG GGACTGTGGT 
GTGGGAAGCC TTGGTGAAGC CAGGAGATCT CAGACGGGCA GTTACTCCAG TTCCTCCAGG 
CCTCAGAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025136565.1
CDS	693..3890
Translation	MQLLLLHTPYHKDYESLNRESWDHGVVEGQGHLIEVRDLFGSDLGPQEGPHTVILHGVAGIGKSTLARHIRRAWEEGQLYRNHFQHVFYFNCRELAMSKLTSLEELITKDQSTTVAPAGQVQSQPEQLLFILDGLDELEWVSEEQRAELCLHRSQQQPVQTLLGSLVEKTIFPEASLLITARTAALQKFIPSLKQPCWVEVLGFSESSRKDYFYEYFTEESQATRAFNVVESNQALLTMCLTPLVSWLACTCLKQQMEAGEELSLRSQTMTALYLRYLAQAIQAQPLGTQLRSFCTLAAEGIWQGKTLFTPGDLKKHGLDWVINSTPLRMDVLQKHPTSLNYSFIHLCFQEFFAAVSFALGDMGDKSDHPNSTGSMEKLVEVYGINNLFGAPTVHFLSGLLSEQGAIEMENIFQCKLSWERKRDSLLQWAELELGREHYSLQLFHCLYEIQDEEFLTQAMAHFQGTRVCIRTSMELLVFTFCIRFCSHVRQLQLNEGRQHPGACRPTDTVLLSWVPATEACWQVLCSVLQVTGNLKELDLSGNILSRSAVQSLDEALRCPGCQLETLRLASCGLTAESCKDIASGLSSSQTLMQLELSFNTLLDAGAEHLCQGLRQPTCKLQRLLLAGCGLTSGCCQDLASMLRVSPSLMELNLLQNDLDNLGVRLLCEGLRHPSCQLICLWLDQTQLSEEVMEMLKALEREKPQLLVSGIWKPSLRILHEGPGGAEMSDTSSLKRQRQASEGSSPQAAQVRLFCPSSPAPLEDKHMESLGTENDLWGPTGPVATQAVDEERSLYRVHLPVAGFYHWPNTGLRFEVRGPVTVEIEFCAWDQFLNRNFPQHSWLAAGPLFDIKAEPGAVAAVFLPHFVAFRGNDVDISQFQVAHFKEEGMLLEKPARVTPCYAVLENPSFSPMGVLLRMVQTALRFIPITSTVLLYHYLQPEEVTFHLYLIPSDCSIQKAIDNEEKKFQFVRIHKPPPLTPLYMGSRYIVSGSEKLEILPEELELCYRSPRESQLFSEFYVRHLRSGIRLQIRSKKDWDCGVGSLGEARRSQTGSYSSSSRPQSLT

Target ORF information:

RefSeq Version	XM_025280780.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NLR family pyrin domain containing 1 (NLRP1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025280780.1

ORF Insert Sequence:

ATGCAGTTGC TACTTCTACA CACTCCTTAC CACAAAGACT ATGAGTCCTT GAACCGGGAA 
AGCTGGGATC ACGGGGTGGT TGAGGGGCAA GGGCATTTGA TTGAGGTCAG AGACTTATTT 
GGCTCAGACC TGGGTCCCCA GGAAGGGCCT CACACAGTCA TATTGCATGG AGTTGCTGGA 
ATTGGGAAGT CCACACTGGC CAGGCACATA AGAAGAGCCT GGGAGGAGGG CCAGCTATAC 
AGGAACCACT TCCAGCATGT CTTCTACTTC AACTGCAGAG AGCTGGCCAT GTCCAAGCTG 
ACAAGTCTGG AGGAACTCAT CACAAAAGAC CAGTCCACCA CTGTGGCTCC CGCTGGGCAG 
GTCCAGTCTC AGCCGGAGCA GCTGCTCTTC ATCCTCGATG GTTTAGATGA ACTGGAATGG 
GTCTCAGAGG AGCAGAGGGC AGAGCTCTGT CTGCACCGGA GCCAGCAGCA GCCCGTGCAG 
ACACTGCTGG GCAGTTTGGT GGAGAAAACC ATATTTCCAG AGGCATCCTT GCTGATCACA 
GCTCGGACTG CAGCTCTGCA GAAGTTCATT CCTTCTTTGA AGCAGCCATG TTGGGTGGAG 
GTCCTAGGGT TCTCTGAGTC TTCCAGGAAG GACTATTTCT ATGAATATTT CACAGAGGAA 
AGCCAAGCAA CTAGAGCCTT TAACGTGGTT GAATCAAACC AAGCACTCTT GACCATGTGT 
CTCACGCCCT TGGTGTCCTG GCTGGCCTGC ACTTGTCTGA AGCAGCAGAT GGAGGCAGGG 
GAAGAACTCT CACTGAGGTC ACAGACCATG ACAGCCCTCT ATCTGCGCTA CCTTGCCCAA 
GCTATCCAAG CTCAGCCCCT GGGGACTCAG CTGAGGAGCT TCTGTACTCT TGCTGCTGAG 
GGTATCTGGC AAGGAAAGAC TCTGTTCACT CCAGGGGACC TCAAGAAGCA CGGATTAGAT 
TGGGTCATCA ACTCCACTCC CTTAAGGATG GATGTTCTTC AAAAGCACCC CACGTCTCTG 
AACTACAGCT TCATTCACCT GTGTTTCCAG GAGTTCTTTG CAGCAGTGTC CTTTGCCTTA 
GGGGACATGG GGGACAAAAG TGACCATCCT AACAGCACTG GAAGCATGGA AAAGTTGGTA 
GAAGTATATG GGATCAATAA CCTGTTTGGG GCACCAACCG TGCATTTCCT ATCCGGCCTT 
TTGAGTGAGC AAGGAGCGAT AGAGATGGAG AACATTTTCC AATGCAAGCT GTCTTGGGAG 
AGAAAGCGGG ATTCACTGCT GCAGTGGGCT GAATTGGAAC TCGGGCGTGA GCATTACTCT 
CTGCAATTGT TTCACTGTTT GTACGAGATT CAGGACGAGG AGTTCCTGAC ACAAGCGATG 
GCCCATTTCC AAGGAACAAG AGTGTGCATC CGAACCAGCA TGGAGCTGCT CGTGTTCACT 
TTCTGCATTA GATTCTGCAG CCACGTGCGG CAGCTTCAAC TGAATGAGGG CAGACAACAT 
CCAGGAGCGT GCAGGCCCAC CGACACAGTG CTGTTGAGCT GGGTCCCGGC CACAGAGGCC 
TGCTGGCAGG TTCTCTGCTC CGTTCTCCAG GTCACTGGAA ACCTGAAGGA ACTGGACCTG 
AGTGGAAACA TCTTGAGCCG CTCTGCAGTA CAGAGTCTTG ATGAGGCCCT GAGATGCCCT 
GGGTGTCAGC TAGAGACCCT GCGGCTGGCC AGCTGCGGCC TCACAGCTGA GAGCTGCAAG 
GACATTGCCT CTGGGCTGAG CTCCAGCCAA ACCCTGATGC AATTGGAATT GAGCTTCAAC 
ACACTCCTGG ACGCAGGAGC TGAGCACCTT TGCCAGGGCC TGAGACAGCC AACCTGCAAG 
CTGCAGCGAC TGTTGCTGGC AGGCTGCGGC CTCACGTCTG GCTGCTGCCA GGACCTGGCC 
TCCATGCTCA GGGTCAGCCC CAGCCTGATG GAGCTAAACC TGCTGCAGAA TGACCTGGAC 
AACCTCGGCG TGAGGCTGCT CTGTGAAGGA CTCAGGCACC CTTCCTGCCA GCTCATATGC 
CTATGGCTGG ACCAGACTCA GCTGAGCGAG GAGGTGATGG AGATGCTGAA GGCCCTGGAG 
AGGGAGAAGC CTCAGCTGCT AGTCTCCGGG ATATGGAAGC CAAGTCTGAG GATCCTTCAC 
GAGGGCCCAG GTGGAGCAGA GATGAGCGAT ACATCCTCCC TCAAGCGGCA GAGACAAGCA 
TCAGAGGGCA GCTCTCCTCA AGCAGCACAG GTGAGACTCT TCTGTCCGTC TTCTCCTGCC 
CCTCTGGAGG ACAAGCACAT GGAGTCTCTG GGGACCGAGA ATGACCTCTG GGGCCCCACA 
GGGCCTGTGG CTACCCAGGC AGTTGACGAA GAGAGGAGCC TGTACCGCGT TCACTTGCCC 
GTGGCCGGCT TCTACCACTG GCCCAACACG GGTCTCCGCT TTGAGGTGAG AGGGCCAGTG 
ACCGTTGAAA TTGAATTCTG TGCCTGGGAC CAGTTCCTGA ACAGGAATTT CCCACAGCAC 
AGCTGGCTGG CTGCAGGGCC TCTGTTTGAC ATCAAGGCTG AACCAGGCGC TGTGGCAGCC 
GTGTTCCTCC CTCACTTTGT GGCCTTCCGA GGGAACGATG TGGACATCTC CCAGTTTCAA 
GTGGCCCACT TCAAAGAGGA GGGGATGCTC CTGGAGAAGC CAGCCAGGGT GACGCCATGT 
TACGCTGTCC TGGAAAACCC CAGCTTCTCG CCCATGGGAG TTCTACTGAG GATGGTCCAA 
ACTGCCCTGC GCTTCATTCC CATCACCTCC ACTGTGTTGC TCTACCATTA CCTCCAGCCT 
GAAGAAGTTA CCTTCCACCT CTACCTGATC CCCAGCGATT GCTCCATTCA GAAGGCCATA 
GACAATGAAG AAAAGAAGTT CCAGTTTGTG CGAATTCACA AGCCACCCCC GCTGACCCCA 
CTCTACATGG GCTCCCGCTA CATTGTGTCT GGTTCAGAGA AGCTGGAAAT ACTGCCCGAG 
GAATTGGAGC TCTGCTATCG GAGCCCCAGA GAATCTCAGC TGTTCTCTGA GTTCTACGTT 
CGCCATTTGA GGTCAGGGAT CAGGTTGCAA ATAAGAAGCA AGAAAGACTG GGACTGTGGT 
GTGGGAAGCC TTGGTGAAGC CAGGAGATCT CAGACGGGCA GTTACTCCAG TTCCTCCAGG 
CCTCAGAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NLRP1
Entrez Gene ID	102402911
Full Name	NLR family pyrin domain containing 1
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NLRP1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

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