TJP1 gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006066395.1	XP_006066457.1	tight junction protein ZO-1 isoform X3
XM_006066397.1	XP_006066459.1	tight junction protein ZO-1 isoform X5
XM_025271183.1	XP_025126968.1	tight junction protein ZO-1 isoform X3
XM_006066396.1	XP_006066458.1	tight junction protein ZO-1 isoform X4
XM_025271184.1	XP_025126969.1	tight junction protein ZO-1 isoform X4
XM_006066396.2	XP_006066458.1	tight junction protein ZO-1 isoform X5
XM_025271186.1	XP_025126971.1	tight junction protein ZO-1 isoform X7
XM_006066393.1	XP_006066455.1	tight junction protein ZO-1 isoform X1
XM_006066397.2	XP_006066459.1	tight junction protein ZO-1 isoform X6
XM_006066394.1	XP_006066456.1	tight junction protein ZO-1 isoform X2
XM_006066398.1	XP_006066460.1	tight junction protein ZO-1 isoform X6
XM_006066399.1	XP_006066461.1	tight junction protein ZO-1 isoform X7
XM_025271181.1	XP_025126966.1	tight junction protein ZO-1 isoform X1
XM_025271182.1	XP_025126967.1	tight junction protein ZO-1 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu21755	XM_006066393.1 Latest version!	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu21756	XM_006066394.1 Latest version!	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu21757	XM_006066395.1 Latest version!	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu21758	XM_006066396.2 Latest version!	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu21759	XM_006066397.2 Latest version!	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu21760	XM_006066398.1 Latest version!	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu21761	XM_006066399.1 Latest version!	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu56738	XM_025271181.1 Latest version!	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu56739	XM_025271182.1 Latest version!	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu56740	XM_025271183.1 Latest version!	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu56741	XM_025271184.1 Latest version!	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu56742	XM_025271186.1 Latest version!	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OBu21758	XM_006066396.1	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu21759	XM_006066397.1	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

TJP1 ( XM_006066393.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006066455.1 Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu21755
Clone ID Related Accession (Same CDS sequence)	XM_006066393.1
Accession Version	XM_006066393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	tight junction protein ZO-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785206.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACATG CTGCGTATTT TATCTCTCTT CGCCCCCGCC GCCCCATGGT AACGCCGTCC 
TCTGAAAACC CCAGTGGTGC TACTTCTAGT GTCAGCCAAG GAAAGCCTTC TTTAAGACGA 
ATTAAAGGGA GACTGCACAG GAGCAAGAGC CTCGATAGCA TCGATTTCTG TGAACTCACT 
AACACAACAA TGGAGGAAAC AGCTATATGG GAACAACACA CGGTGACACT TCACAGGGCT 
CCTGGATTTG GATTTGGAAT TGCAATATCT GGTGGAAGAG ATAATCCACA TTTCCAGAGC 
GGGGAAACCT CCATCGTGAT TTCAGATGTG CTAAAGGGGG GGCCTGCAGA AGGACAGCTG 
CAGGAAAATG ACCGGGTGGC AATGGTTAAT GGAGTCTCCA TGGATAATGT TGAGCATGCG 
TTTGCTGTTC AGCAACTAAG GAAAAGTGGG AAAAATGCAA AAATTACCAT TCGAAGGAAG 
AAGAAAGTTC AGATCCCAGT AAATCGTCCT GATCCTGAGC CTGTGTCTGA GAACGAAGAG 
GACAGTTACG ACGAGGAGGC GCATGACCCC AGAAGTGGCC GTGGGGCCCT GGCAAACAGG 
AGGAGCGAGA AGAGCTGGGG CCGAGATAGG AGTGCCAGTC GGGAGAGGAG TCTGTCCCCG 
AGGTCTGACA GGCGGTCTGT GGCCTCCAGT CAGCCTGCCA GACCCACCAA AGTCACCCTG 
GTGAAGTCCC GGAAGAATGA AGAATATGGT CTTCGATTGG CCAGCCATAT ATTTGTGAAG 
GAAATTTCAC AAGATAGTTT GGCAGCAAGA GATGGCAATA TTCAAGAAGG CGATGTTGTA 
TTGAAGATAA ATGGTACCGT GACGGAAAAC ATGTCACTGA CAGATGCGAA GACGCTGATC 
GAGAGGTCTA AGGGCAAGTT AAAGATGGTG GTTCAGAGAG ATGAGCGGGC TACCCTGCTG 
AATGTCCCTG ACCTTTCTGA CAGCATCCAC TCTGCTGACG CCTCCGAGAG GGACGATATT 
TCAGAAATCC AGTCACTGGC ATCAGACCAT TCGGGTCGAT CCCACGACCG GCCTCCCCGT 
CACAGCCGCT CTCGGTCTCC TGACCAGCGG TCCGAGCCTT CTGACCATTC TAGACACTCT 
CCACAGCAGC CCAGCAGTGG CAGTCTCCGG AGCAGAGAGG AAGAGAGAAT TTCTAAACCA 
GGGGCTGTCT CGACTCCGGT AAAGCATGCA GATGATCACA CACCTAAAAC AGTGGAAGAA 
GTTGTAGTTG AGCGAAATGA GAAACAAGCA CCCAATCTTC CAGAACCAAA GCCTGTGTAT 
GCCCAGGTAG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG TGTCCTGCCC 
AACTCAACTC ATGAAGATGG CATTCTTCGG CCCAGCATGA AGTTAGTAAA ATTCAGGAAA 
GGAGATAGTG TGGGTCTTCG GCTAGCTGGT GGAAACGATG TTGGCATATT TGTAGCTGGT 
GTTCTCGAAG ACAGCCCTGC TGCCAAAGAA GGCTTGGAGG AGGGCGACCA GATCCTCAGG 
GTAAACAATG TAGATTTCAC AAATATCATA AGAGAAGAAG CCGTCCTCTT CCTGCTTGAC 
CTCCCAAAAG GAGAAGAAGT GACCATATTG GCTCAGAAGA AAAAAGATGT TTATCGTCGC 
ATCGTAGAAT CCGATGTGGG TGATTCTTTC TATATCAGAA CCCATTTTGA ATATGAGAAG 
GAATCTCCCT ATGGACTCAG TTTTAACAAA GGAGAGGTGT TCCGTGTCGT GGACACTCTG 
TACAATGGGA AGCTGGGGTC CTGGCTCGCC ATTCGGATTG GCAAAAACCA TAAAGAGGTG 
GAACGTGGCA TCATCCCAAA CAAAAACAGA GCTGAGCAGT TAGCCAGTGT GCAGTATACA 
CTTCCCAAGA CAGCAGGGGG AGACCGCGCT GACTTCTGGA GGTTCCGCGG TCTGCGCAGC 
TCCAAGAGAA ACCTGCGCAA GAGCCGAGAA GACCTCTCAG CCCAGCCAGT CCAGACCAAG 
TTCCCGGCGT ATGAGAGGGT GGTTCTTCGA GAAGCTGGAT TCCTGAGGCC TGTAACCATC 
TTTGGACCAA TAGCTGATGT TGCCAGGGAG AAGCTGGCAA GAGAGGAGCC AGATATTTAC 
CAGATCGCAA AAAGTGAACC TCGGGATGCA GGAACCGACC AGCGCAGCTC TGGCATTATT 
CGCCTCCACA CAATAAAGCA GATAATAGAT CAGGACAAAC ATGCCTTGCT GGATGTAACG 
CCCAATGCAG TAGACCGTCT GAACTATGCC CAGTGGTATC CAATAGTGGT GTTCCTCAAC 
CCGGACTCAA AGCAGGGTGT GAAAACAATG CGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGCGCCAGGA AGTTATATGA GCGCTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGCTGGT ATGGTGCATT GAAAGAAGCA 
ATCCAGCAAC AACAGAACCA GTTGGTATGG GTTTCTGAGG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAGACCCTAA ATGATGAGGT GGGGACTCCC 
CCGGAGTCTG CCATTACCCG GTCCTCTGAG CCTGTCAGAG AGGACTCCTC TGGAATGCAT 
CCTGACCACC AGGCATATCC TCCCTACTCA CCACAAGCGC AGCCACAGCC AGGCCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGGC 
CCTTACCTTT CGCCTGAAAC AAACCCGGCA CCATCAGCCT CTGCTGTTAA TCCTCACGTA 
GACTTAACTA ATGTCAGACT GGAGGAGCCG AGCCCAGCTC CCCCTTCCTC TGACTCACCA 
CACGCCGACT CTTTACGAGC TCCAGGCACT GAGGCCGCTC ACGTGCAGCT CAGAGGTCCA 
GAGCCGTCCC TGTCGTCGCA TGTGGGCCCA GCAAAGGTGT ACAGGAAGGA CCCCTACCCT 
GAGGAGATGA TGAGACAGAA CCACGTCTTG AAGCAGCCGG CTGCGGTTCA CCCTGGGCAG 
AGGCCAGACA AGGAGCAAGG CCTGAGCTAC GAGCCGCAGC CTCCGTACAC AGAGCGACAG 
GCTGGCAGGG AGTTGGAGCA GCCTGCATAC AGATATGATG CCTCTAGCTA CCCGGAGCAG 
TTTGCTCGGA ACTATGACCA TCGTCTGCGC TATGATGACC GCCTCCTCAC CTACGAGGAG 
CAGTGGCCCT ACTACGACGA CAAGCAGCCT TACCAGCCCC GGCCTCTTGA TGGTCAGCCT 
CCCGCGGAGC CAACAGAACG AGGGTATTTT CCCCGTTTTG AGGAGCCAGT GCCCCTGTCC 
TATGAAGGCC GAGCGCACTA TGACCCACTG CCCAGGACCT CCACCTTGCG GCACGAAGAG 
CCTCCTGCCC CTGCCCCTGC CCCTGGGTAC GACGTGCACG GCAGGTACCG GCCTGACGCG 
CAGCCCTACT CCACTGCTGG CCCCAAGGTG CCCCCTGAGC CCAAGCCGTA CTTGGACCAG 
TACCCTCGGA GTTACGAGCC AGCGCCAGCC CAGGGCTTCC CCTCCAGGGC AGGCCCAGGC 
CACTACGAGC CTCTTCACAG CACCACGCTC GCTCCACCAC TTGGCCCCTC GTCCCAGCAC 
AAGCCCGAAG TCCCGCCCAC AGGCTCCAAA CCCCTGCCTC CACCCCCAGC TCCAGCTGAG 
GAGGAGGAAG ACCCAGCCAT GAAGCCACAA TCCGTGCTCA CCAGAGTGAA GATGTTTGAG 
AACAAGAGGT CTGCTTCCAT GGAGAACAGG AAGGATGAGA GCCATTCCAC TAGTTTCAAG 
CCCTCAGAAG TCACGTCAAA ACCGGCAGGT GCTCCTGTCC CAGGTCCCAA AGCCCCTGCT 
CCGGGTCAGC TCGGTGAACA CGACAGAGCA GCGCATAGGA TTCCTGAACC ACAGAAGCCT 
CACGTGAAGC CACCTGAAGA CATTGTTCGG GCAAATCACT ATGACCCCGA GGAGGATGAG 
GAGTACTACC GGAAGCAGCT CTCCTACTTC GATCGGAGAA GCTTTGAGGC CAAGCCCCCC 
ACCCACATCC CCACTAGCCA CCTCTCCGAG CCCACCAGGC CGGCTCACCC CCAATCTCAG 
CCACAGTTTG CCGGTTACTC TTCCAAGGGG AAGGCTGCTG AGGCCGAAGC TGTGGACAGG 
TCATTCGGGG AGAAGCGCTA TGACCCGCTG CAGGCGACGC CCCCCCCTCC GCCGCTGCCC 
GCTCAGTACA GCCAGACTGC TCAACCCAGC CCCAACACGG CTCTCGGCCT CCACGCACAC 
ACCAAGGGGG CGCACGGTGA AGGTAATGCC ATGTCATTGG ATTTTCAGAA CTCGTTAGTG 
TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAG CTTACAGACC AGCCCCTCGA 
GAGGATGCTG TCCAGTCTGC CTTCTACCCA CAAAAGAGTT TCCCAGAGAA AGCCCCAGCT 
AATGGGGTCG AGCAGACGCA GAAAACCGTC ACGCCAGCAT ACAATCGATT CACACCAAAA 
CCATACACAA GCTCTGCACG GCCGTTTGAA CGCAAGTTTG AAAGCCCTAA ATTCAACCAC 
AATCTCTTGC CGAATGAAAC CGCACACAAA CCTGACCTGT CCTCCAAAGC CCCTGCTTCT 
CCGAAAACCC TCCTGAAGTC TGCACAGCCT CCTGAGTTTG ACAGTGGTGT GGAGACCTTC 
TCTGTCCACA CAGACAACAA GCTGAAATAC CAAGTAAACA GTGTTAGCAC GGGGCCGAGA 
GCAGTGCCCG TGAGTCCCTC AGCCGTGGAG GAAGATGAAG ATGAAGACGG TCACACAGTG 
GTGGCTACTG CCCGCGGCGT GTTTAACAGC AATGGTGGGG TGCTGAGCTC CATTGAGACC 
GGTGTCAGCA TCATCATCCC CCAGGGAGCC ATTCCTGAGG GGGTGGAACA GGAGATCTAC 
TTCAAGGTCT GCCGCGACAA CAGCATCCTG CCGCCTTTGG ACAAAGAGAA GGGTGAGACC 
CTGCTGAGCC CCCTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCCTAC CACACTGTGC GTCCATGACT CCTGACGGTT GGTCTTTTGC TCTAAAATCA 
TCCGACTCCT CGTCGGGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTATCTTG TTGGAGCAAA CTGTGTTTCT GTCCTGATTG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006066455.1
CDS	1..5451
Translation	MEHAAYFISLRPRRPMVTPSSENPSGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTTMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVNRPDPEPVSENEEDSYDEEAHDPRSGRGALANRRSEKSWGRDRSASRERSLSPRSDRRSVASSQPARPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSADASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTVEEVVVERNEKQAPNLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHPDHQAYPPYSPQAQPQPGHRIDSPGFKTASQQKAEASSPGPYLSPETNPAPSASAVNPHVDLTNVRLEEPSPAPPSSDSPHADSLRAPGTEAAHVQLRGPEPSLSSHVGPAKVYRKDPYPEEMMRQNHVLKQPAAVHPGQRPDKEQGLSYEPQPPYTERQAGRELEQPAYRYDASSYPEQFARNYDHRLRYDDRLLTYEEQWPYYDDKQPYQPRPLDGQPPAEPTERGYFPRFEEPVPLSYEGRAHYDPLPRTSTLRHEEPPAPAPAPGYDVHGRYRPDAQPYSTAGPKVPPEPKPYLDQYPRSYEPAPAQGFPSRAGPGHYEPLHSTTLAPPLGPSSQHKPEVPPTGSKPLPPPPAPAEEEEDPAMKPQSVLTRVKMFENKRSASMENRKDESHSTSFKPSEVTSKPAGAPVPGPKAPAPGQLGEHDRAAHRIPEPQKPHVKPPEDIVRANHYDPEEDEEYYRKQLSYFDRRSFEAKPPTHIPTSHLSEPTRPAHPQSQPQFAGYSSKGKAAEAEAVDRSFGEKRYDPLQATPPPPPLPAQYSQTAQPSPNTALGLHAHTKGAHGEGNAMSLDFQNSLVSKPDPPPSQNKPAAYRPAPREDAVQSAFYPQKSFPEKAPANGVEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPNETAHKPDLSSKAPASPKTLLKSAQPPEFDSGVETFSVHTDNKLKYQVNSVSTGPRAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006066393.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006066393.1

ORF Insert Sequence:

ATGGAACATG CTGCGTATTT TATCTCTCTT CGCCCCCGCC GCCCCATGGT AACGCCGTCC 
TCTGAAAACC CCAGTGGTGC TACTTCTAGT GTCAGCCAAG GAAAGCCTTC TTTAAGACGA 
ATTAAAGGGA GACTGCACAG GAGCAAGAGC CTCGATAGCA TCGATTTCTG TGAACTCACT 
AACACAACAA TGGAGGAAAC AGCTATATGG GAACAACACA CGGTGACACT TCACAGGGCT 
CCTGGATTTG GATTTGGAAT TGCAATATCT GGTGGAAGAG ATAATCCACA TTTCCAGAGC 
GGGGAAACCT CCATCGTGAT TTCAGATGTG CTAAAGGGGG GGCCTGCAGA AGGACAGCTG 
CAGGAAAATG ACCGGGTGGC AATGGTTAAT GGAGTCTCCA TGGATAATGT TGAGCATGCG 
TTTGCTGTTC AGCAACTAAG GAAAAGTGGG AAAAATGCAA AAATTACCAT TCGAAGGAAG 
AAGAAAGTTC AGATCCCAGT AAATCGTCCT GATCCTGAGC CTGTGTCTGA GAACGAAGAG 
GACAGTTACG ACGAGGAGGC GCATGACCCC AGAAGTGGCC GTGGGGCCCT GGCAAACAGG 
AGGAGCGAGA AGAGCTGGGG CCGAGATAGG AGTGCCAGTC GGGAGAGGAG TCTGTCCCCG 
AGGTCTGACA GGCGGTCTGT GGCCTCCAGT CAGCCTGCCA GACCCACCAA AGTCACCCTG 
GTGAAGTCCC GGAAGAATGA AGAATATGGT CTTCGATTGG CCAGCCATAT ATTTGTGAAG 
GAAATTTCAC AAGATAGTTT GGCAGCAAGA GATGGCAATA TTCAAGAAGG CGATGTTGTA 
TTGAAGATAA ATGGTACCGT GACGGAAAAC ATGTCACTGA CAGATGCGAA GACGCTGATC 
GAGAGGTCTA AGGGCAAGTT AAAGATGGTG GTTCAGAGAG ATGAGCGGGC TACCCTGCTG 
AATGTCCCTG ACCTTTCTGA CAGCATCCAC TCTGCTGACG CCTCCGAGAG GGACGATATT 
TCAGAAATCC AGTCACTGGC ATCAGACCAT TCGGGTCGAT CCCACGACCG GCCTCCCCGT 
CACAGCCGCT CTCGGTCTCC TGACCAGCGG TCCGAGCCTT CTGACCATTC TAGACACTCT 
CCACAGCAGC CCAGCAGTGG CAGTCTCCGG AGCAGAGAGG AAGAGAGAAT TTCTAAACCA 
GGGGCTGTCT CGACTCCGGT AAAGCATGCA GATGATCACA CACCTAAAAC AGTGGAAGAA 
GTTGTAGTTG AGCGAAATGA GAAACAAGCA CCCAATCTTC CAGAACCAAA GCCTGTGTAT 
GCCCAGGTAG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG TGTCCTGCCC 
AACTCAACTC ATGAAGATGG CATTCTTCGG CCCAGCATGA AGTTAGTAAA ATTCAGGAAA 
GGAGATAGTG TGGGTCTTCG GCTAGCTGGT GGAAACGATG TTGGCATATT TGTAGCTGGT 
GTTCTCGAAG ACAGCCCTGC TGCCAAAGAA GGCTTGGAGG AGGGCGACCA GATCCTCAGG 
GTAAACAATG TAGATTTCAC AAATATCATA AGAGAAGAAG CCGTCCTCTT CCTGCTTGAC 
CTCCCAAAAG GAGAAGAAGT GACCATATTG GCTCAGAAGA AAAAAGATGT TTATCGTCGC 
ATCGTAGAAT CCGATGTGGG TGATTCTTTC TATATCAGAA CCCATTTTGA ATATGAGAAG 
GAATCTCCCT ATGGACTCAG TTTTAACAAA GGAGAGGTGT TCCGTGTCGT GGACACTCTG 
TACAATGGGA AGCTGGGGTC CTGGCTCGCC ATTCGGATTG GCAAAAACCA TAAAGAGGTG 
GAACGTGGCA TCATCCCAAA CAAAAACAGA GCTGAGCAGT TAGCCAGTGT GCAGTATACA 
CTTCCCAAGA CAGCAGGGGG AGACCGCGCT GACTTCTGGA GGTTCCGCGG TCTGCGCAGC 
TCCAAGAGAA ACCTGCGCAA GAGCCGAGAA GACCTCTCAG CCCAGCCAGT CCAGACCAAG 
TTCCCGGCGT ATGAGAGGGT GGTTCTTCGA GAAGCTGGAT TCCTGAGGCC TGTAACCATC 
TTTGGACCAA TAGCTGATGT TGCCAGGGAG AAGCTGGCAA GAGAGGAGCC AGATATTTAC 
CAGATCGCAA AAAGTGAACC TCGGGATGCA GGAACCGACC AGCGCAGCTC TGGCATTATT 
CGCCTCCACA CAATAAAGCA GATAATAGAT CAGGACAAAC ATGCCTTGCT GGATGTAACG 
CCCAATGCAG TAGACCGTCT GAACTATGCC CAGTGGTATC CAATAGTGGT GTTCCTCAAC 
CCGGACTCAA AGCAGGGTGT GAAAACAATG CGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGCGCCAGGA AGTTATATGA GCGCTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGCTGGT ATGGTGCATT GAAAGAAGCA 
ATCCAGCAAC AACAGAACCA GTTGGTATGG GTTTCTGAGG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAGACCCTAA ATGATGAGGT GGGGACTCCC 
CCGGAGTCTG CCATTACCCG GTCCTCTGAG CCTGTCAGAG AGGACTCCTC TGGAATGCAT 
CCTGACCACC AGGCATATCC TCCCTACTCA CCACAAGCGC AGCCACAGCC AGGCCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGGC 
CCTTACCTTT CGCCTGAAAC AAACCCGGCA CCATCAGCCT CTGCTGTTAA TCCTCACGTA 
GACTTAACTA ATGTCAGACT GGAGGAGCCG AGCCCAGCTC CCCCTTCCTC TGACTCACCA 
CACGCCGACT CTTTACGAGC TCCAGGCACT GAGGCCGCTC ACGTGCAGCT CAGAGGTCCA 
GAGCCGTCCC TGTCGTCGCA TGTGGGCCCA GCAAAGGTGT ACAGGAAGGA CCCCTACCCT 
GAGGAGATGA TGAGACAGAA CCACGTCTTG AAGCAGCCGG CTGCGGTTCA CCCTGGGCAG 
AGGCCAGACA AGGAGCAAGG CCTGAGCTAC GAGCCGCAGC CTCCGTACAC AGAGCGACAG 
GCTGGCAGGG AGTTGGAGCA GCCTGCATAC AGATATGATG CCTCTAGCTA CCCGGAGCAG 
TTTGCTCGGA ACTATGACCA TCGTCTGCGC TATGATGACC GCCTCCTCAC CTACGAGGAG 
CAGTGGCCCT ACTACGACGA CAAGCAGCCT TACCAGCCCC GGCCTCTTGA TGGTCAGCCT 
CCCGCGGAGC CAACAGAACG AGGGTATTTT CCCCGTTTTG AGGAGCCAGT GCCCCTGTCC 
TATGAAGGCC GAGCGCACTA TGACCCACTG CCCAGGACCT CCACCTTGCG GCACGAAGAG 
CCTCCTGCCC CTGCCCCTGC CCCTGGGTAC GACGTGCACG GCAGGTACCG GCCTGACGCG 
CAGCCCTACT CCACTGCTGG CCCCAAGGTG CCCCCTGAGC CCAAGCCGTA CTTGGACCAG 
TACCCTCGGA GTTACGAGCC AGCGCCAGCC CAGGGCTTCC CCTCCAGGGC AGGCCCAGGC 
CACTACGAGC CTCTTCACAG CACCACGCTC GCTCCACCAC TTGGCCCCTC GTCCCAGCAC 
AAGCCCGAAG TCCCGCCCAC AGGCTCCAAA CCCCTGCCTC CACCCCCAGC TCCAGCTGAG 
GAGGAGGAAG ACCCAGCCAT GAAGCCACAA TCCGTGCTCA CCAGAGTGAA GATGTTTGAG 
AACAAGAGGT CTGCTTCCAT GGAGAACAGG AAGGATGAGA GCCATTCCAC TAGTTTCAAG 
CCCTCAGAAG TCACGTCAAA ACCGGCAGGT GCTCCTGTCC CAGGTCCCAA AGCCCCTGCT 
CCGGGTCAGC TCGGTGAACA CGACAGAGCA GCGCATAGGA TTCCTGAACC ACAGAAGCCT 
CACGTGAAGC CACCTGAAGA CATTGTTCGG GCAAATCACT ATGACCCCGA GGAGGATGAG 
GAGTACTACC GGAAGCAGCT CTCCTACTTC GATCGGAGAA GCTTTGAGGC CAAGCCCCCC 
ACCCACATCC CCACTAGCCA CCTCTCCGAG CCCACCAGGC CGGCTCACCC CCAATCTCAG 
CCACAGTTTG CCGGTTACTC TTCCAAGGGG AAGGCTGCTG AGGCCGAAGC TGTGGACAGG 
TCATTCGGGG AGAAGCGCTA TGACCCGCTG CAGGCGACGC CCCCCCCTCC GCCGCTGCCC 
GCTCAGTACA GCCAGACTGC TCAACCCAGC CCCAACACGG CTCTCGGCCT CCACGCACAC 
ACCAAGGGGG CGCACGGTGA AGGTAATGCC ATGTCATTGG ATTTTCAGAA CTCGTTAGTG 
TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAG CTTACAGACC AGCCCCTCGA 
GAGGATGCTG TCCAGTCTGC CTTCTACCCA CAAAAGAGTT TCCCAGAGAA AGCCCCAGCT 
AATGGGGTCG AGCAGACGCA GAAAACCGTC ACGCCAGCAT ACAATCGATT CACACCAAAA 
CCATACACAA GCTCTGCACG GCCGTTTGAA CGCAAGTTTG AAAGCCCTAA ATTCAACCAC 
AATCTCTTGC CGAATGAAAC CGCACACAAA CCTGACCTGT CCTCCAAAGC CCCTGCTTCT 
CCGAAAACCC TCCTGAAGTC TGCACAGCCT CCTGAGTTTG ACAGTGGTGT GGAGACCTTC 
TCTGTCCACA CAGACAACAA GCTGAAATAC CAAGTAAACA GTGTTAGCAC GGGGCCGAGA 
GCAGTGCCCG TGAGTCCCTC AGCCGTGGAG GAAGATGAAG ATGAAGACGG TCACACAGTG 
GTGGCTACTG CCCGCGGCGT GTTTAACAGC AATGGTGGGG TGCTGAGCTC CATTGAGACC 
GGTGTCAGCA TCATCATCCC CCAGGGAGCC ATTCCTGAGG GGGTGGAACA GGAGATCTAC 
TTCAAGGTCT GCCGCGACAA CAGCATCCTG CCGCCTTTGG ACAAAGAGAA GGGTGAGACC 
CTGCTGAGCC CCCTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCCTAC CACACTGTGC GTCCATGACT CCTGACGGTT GGTCTTTTGC TCTAAAATCA 
TCCGACTCCT CGTCGGGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTATCTTG TTGGAGCAAA CTGTGTTTCT GTCCTGATTG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_006066394.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006066456.1 Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X2, mRNA.

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CloneID	OBu21756
Clone ID Related Accession (Same CDS sequence)	XM_006066394.1
Accession Version	XM_006066394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	tight junction protein ZO-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785206.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACATG CTGCGTATTT TATCTCTCTT CGCCCCCGCC GCCCCATGGT AACGCCGTCC 
TCTGAAAACC CCAGTGGTGC TACTTCTAGT GTCAGCCAAG GAAAGCCTTC TTTAAGACGA 
ATTAAAGGGA GACTGCACAG GAGCAAGAGC CTCGATAGCA TCGATTTCTG TGAACTCACT 
AACACAACAA TGGAGGAAAC AGCTATATGG GAACAACACA CGGTGACACT TCACAGGGCT 
CCTGGATTTG GATTTGGAAT TGCAATATCT GGTGGAAGAG ATAATCCACA TTTCCAGAGC 
GGGGAAACCT CCATCGTGAT TTCAGATGTG CTAAAGGGGG GGCCTGCAGA AGGACAGCTG 
CAGGAAAATG ACCGGGTGGC AATGGTTAAT GGAGTCTCCA TGGATAATGT TGAGCATGCG 
TTTGCTGTTC AGCAACTAAG GAAAAGTGGG AAAAATGCAA AAATTACCAT TCGAAGGAAG 
AAGAAAGTTC AGATCCCAGT AAATCGTCCT GATCCTGAGC CTGTGTCTGA GAACGAAGAG 
GACAGTTACG ACGAGGAGGC GCATGACCCC AGAAGTGGCC GTGGGGCCCT GGCAAACAGG 
AGGAGCGAGA AGAGCTGGGG CCGAGATAGG AGTGCCAGTC GGGAGAGGAG TCTGTCCCCG 
AGGTCTGACA GGCGGTCTGT GGCCTCCAGT CAGCCTGCCA GACCCACCAA AGTCACCCTG 
GTGAAGTCCC GGAAGAATGA AGAATATGGT CTTCGATTGG CCAGCCATAT ATTTGTGAAG 
GAAATTTCAC AAGATAGTTT GGCAGCAAGA GATGGCAATA TTCAAGAAGG CGATGTTGTA 
TTGAAGATAA ATGGTACCGT GACGGAAAAC ATGTCACTGA CAGATGCGAA GACGCTGATC 
GAGAGGTCTA AGGGCAAGTT AAAGATGGTG GTTCAGAGAG ATGAGCGGGC TACCCTGCTG 
AATGTCCCTG ACCTTTCTGA CAGCATCCAC TCTGCTGACG CCTCCGAGAG GGACGATATT 
TCAGAAATCC AGTCACTGGC ATCAGACCAT TCGGGTCGAT CCCACGACCG GCCTCCCCGT 
CACAGCCGCT CTCGGTCTCC TGACCAGCGG TCCGAGCCTT CTGACCATTC TAGACACTCT 
CCACAGCAGC CCAGCAGTGG CAGTCTCCGG AGCAGAGAGG AAGAGAGAAT TTCTAAACCA 
GGGGCTGTCT CGACTCCGGT AAAGCATGCA GATGATCACA CACCTAAAAC AGTGGAAGAA 
GTTGTAGTTG AGCGAAATGA GAAACAAGCA CCCAATCTTC CAGAACCAAA GCCTGTGTAT 
GCCCAGGTAG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG TGTCCTGCCC 
AACTCAACTC ATGAAGATGG CATTCTTCGG CCCAGCATGA AGTTAGTAAA ATTCAGGAAA 
GGAGATAGTG TGGGTCTTCG GCTAGCTGGT GGAAACGATG TTGGCATATT TGTAGCTGGT 
GTTCTCGAAG ACAGCCCTGC TGCCAAAGAA GGCTTGGAGG AGGGCGACCA GATCCTCAGG 
GTAAACAATG TAGATTTCAC AAATATCATA AGAGAAGAAG CCGTCCTCTT CCTGCTTGAC 
CTCCCAAAAG GAGAAGAAGT GACCATATTG GCTCAGAAGA AAAAAGATGT TTATCGTCGC 
ATCGTAGAAT CCGATGTGGG TGATTCTTTC TATATCAGAA CCCATTTTGA ATATGAGAAG 
GAATCTCCCT ATGGACTCAG TTTTAACAAA GGAGAGGTGT TCCGTGTCGT GGACACTCTG 
TACAATGGGA AGCTGGGGTC CTGGCTCGCC ATTCGGATTG GCAAAAACCA TAAAGAGGTG 
GAACGTGGCA TCATCCCAAA CAAAAACAGA GCTGAGCAGT TAGCCAGTGT GCAGTATACA 
CTTCCCAAGA CAGCAGGGGG AGACCGCGCT GACTTCTGGA GGTTCCGCGG TCTGCGCAGC 
TCCAAGAGAA ACCTGCGCAA GAGCCGAGAA GACCTCTCAG CCCAGCCAGT CCAGACCAAG 
TTCCCGGCGT ATGAGAGGGT GGTTCTTCGA GAAGCTGGAT TCCTGAGGCC TGTAACCATC 
TTTGGACCAA TAGCTGATGT TGCCAGGGAG AAGCTGGCAA GAGAGGAGCC AGATATTTAC 
CAGATCGCAA AAAGTGAACC TCGGGATGCA GGAACCGACC AGCGCAGCTC TGGCATTATT 
CGCCTCCACA CAATAAAGCA GATAATAGAT CAGGACAAAC ATGCCTTGCT GGATGTAACG 
CCCAATGCAG TAGACCGTCT GAACTATGCC CAGTGGTATC CAATAGTGGT GTTCCTCAAC 
CCGGACTCAA AGCAGGGTGT GAAAACAATG CGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGCGCCAGGA AGTTATATGA GCGCTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGCTGGT ATGGTGCATT GAAAGAAGCA 
ATCCAGCAAC AACAGAACCA GTTGGTATGG GTTTCTGAGG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAGACCCTAA ATGATGAGGT GGGGACTCCC 
CCGGAGTCTG CCATTACCCG GTCCTCTGAG CCTGTCAGAG AGGACTCCTC TGGAATGCAT 
CCTGACCACC AGGCATATCC TCCCTACTCA CCACAAGCGC AGCCACAGCC AGGCCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGGC 
CCTTACCTTT CGCCTGAAAC AAACCCGGCA CCATCAGCCT CTGCTGTTAA TCCTCACGTA 
GACTTAACTA ATGTCAGACT GGAGGAGCCG AGCCCAGCTC CCCCTTCCTC TGACTCACCA 
CACGCCGACT CTTTACGAGC TCCAGGCACT GAGGCCGCTC ACGTGCAGCT CAGAGGTCCA 
GAGCCGTCCC TGTCGTCGCA TGTGGGCCCA GCAAAGGTGT ACAGGAAGGA CCCCTACCCT 
GAGGAGATGA TGAGACAGAA CCACGTCTTG AAGCAGCCGG CTGCGGTTCA CCCTGGGCAG 
AGGCCAGACA AGGAGCAAGG CCTGAGCTAC GAGCCGCAGC CTCCGTACAC AGAGCGACAG 
GCTGGCAGGG AGTTGGAGCA GCCTGCATAC AGATATGATG CCTCTAGCTA CCCGGAGCAG 
TTTGCTCGGA ACTATGACCA TCGTCTGCGC TATGATGACC GCCTCCTCAC CTACGAGGAG 
CAGTGGCCCT ACTACGACGA CAAGCAGCCT TACCAGCCCC GGCCTCTTGA TGGTCAGCCT 
CCCGCGGAGC CAACAGAACG AGGGTATTTT CCCCGTTTTG AGGAGCCAGT GCCCCTGTCC 
TATGAAGGCC GAGCGCACTA TGACCCACTG CCCAGGACCT CCACCTTGCG GCACGAAGAG 
CCTCCTGCCC CTGCCCCTGC CCCTGGGTAC GACGTGCACG GCAGGTACCG GCCTGACGCG 
CAGCCCTACT CCACTGCTGG CCCCAAGGTG CCCCCTGAGC CCAAGCCGTA CTTGGACCAG 
TACCCTCGGA GTTACGAGCC AGCGCCAGCC CAGGGCTTCC CCTCCAGGGC AGGCCCAGGC 
CACTACGAGC CTCTTCACAG CACCACGCTC GCTCCACCAC TTGGCCCCTC GTCCCAGCAC 
AAGCCCGAAG TCCCGCCCAC AGGCTCCAAA CCCCTGCCTC CACCCCCAGC TCCAGCTGAG 
GAGGAGGAAG ACCCAGCCAT GAAGCCACAA TCCGTGCTCA CCAGAGTGAA GATGTTTGAG 
AACAAGAGGT CTGCTTCCAT GGAGAACAGG AAGGATGAGA GCCATTCCAC TAGTTTCAAG 
CCCTCAGAAG TCACGTCAAA ACCGGCAGGT GCTCCTGTCC CAGGTCCCAA AGCCCCTGCT 
CCGGGTCAGC TCGGTGAACA CGACAGAGCA GCGCATAGGA TTCCTGAACC ACAGAAGCCT 
CACGTGAAGC CACCTGAAGA CATTGTTCGG GCAAATCACT ATGACCCCGA GGAGGATGAG 
GAGTACTACC GGAAGCAGCT CTCCTACTTC GATCGGAGAA GCTTTGAGGC CAAGCCCCCC 
ACCCACATCC CCACTAGCCA CCTCTCCGAG CCCACCAGGC CGGCTCACCC CCAATCTCAG 
CCACAGTTTG CCGGTTACTC TTCCAAGGGG AAGGCTGCTG AGGCCGAAGC TGTGGACAGG 
TCATTCGGGG AGAAGCGCTA TGACCCGCTG CAGGCGACGC CCCCCCCTCC GCCGCTGCCC 
GCTCAGTACA GCCAGACTGC TCAACCCAGC CCCAACACGG CTCTCGGCCT CCACGCACAC 
ACCAAGGGGG CGCACGGTGA AGGTAATGCC ATGTCATTGG ATTTTCAGAA CTCGTTAGTG 
TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAG CTTACAGACC AGCCCCTCGA 
GAGGATGCTG TCCAGTCTGC CTTCTACCCA CAAAAGAGTT TCCCAGAGAA AGCCCCAGCT 
AATGGGGTCG AGCAGACGCA GAAAACCGTC ACGCCAGCAT ACAATCGATT CACACCAAAA 
CCATACACAA GCTCTGCACG GCCGTTTGAA CGCAAGTTTG AAAGCCCTAA ATTCAACCAC 
AATCTCTTGC CGAATGAAAC CGCACACAAA CCTGACCTGT CCTCCAAAGC CCCTGCTTCT 
CCGAAAACCC TCCTGAAGTC TGCACAGCCT CCTGAGTTTG ACAGTGGTGT GGAGACCTTC 
TCTGTCCACA CAGACAACAA GCTGAAATAC CAAGTAAACA GTGTTAGCAC GGGGCCGAGA 
GCAGTGCCCG TGAGTCCCTC AGCCGTGGAG GAAGATGAAG ATGAAGACGG TCACACAGTG 
GTGGCTACTG CCCGCGGCGT GTTTAACAGC AATGGTGGGG TGCTGAGCTC CATTGAGACC 
GGTGTCAGCA TCATCATCCC CCAGGGAGCC ATTCCTGAGG GGGTGGAACA GGAGATCTAC 
TTCAAGGTCT GCCGCGACAA CAGCATCCTG CCGCCTTTGG ACAAAGAGAA GGGTGAGACC 
CTGCTGAGCC CCCTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCCTAC CACACTGTGC GTCCATGACT CCTGACGGTG ATCCTAAAAC CTGGCAAAAC 
AAGTGTCTTC CTGGAGATCC AAATTATCTT GTTGGAGCAA ACTGTGTTTC TGTCCTGATT 
GACCACTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006066456.1
CDS	1..5412
Translation	MEHAAYFISLRPRRPMVTPSSENPSGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTTMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVNRPDPEPVSENEEDSYDEEAHDPRSGRGALANRRSEKSWGRDRSASRERSLSPRSDRRSVASSQPARPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSADASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTVEEVVVERNEKQAPNLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHPDHQAYPPYSPQAQPQPGHRIDSPGFKTASQQKAEASSPGPYLSPETNPAPSASAVNPHVDLTNVRLEEPSPAPPSSDSPHADSLRAPGTEAAHVQLRGPEPSLSSHVGPAKVYRKDPYPEEMMRQNHVLKQPAAVHPGQRPDKEQGLSYEPQPPYTERQAGRELEQPAYRYDASSYPEQFARNYDHRLRYDDRLLTYEEQWPYYDDKQPYQPRPLDGQPPAEPTERGYFPRFEEPVPLSYEGRAHYDPLPRTSTLRHEEPPAPAPAPGYDVHGRYRPDAQPYSTAGPKVPPEPKPYLDQYPRSYEPAPAQGFPSRAGPGHYEPLHSTTLAPPLGPSSQHKPEVPPTGSKPLPPPPAPAEEEEDPAMKPQSVLTRVKMFENKRSASMENRKDESHSTSFKPSEVTSKPAGAPVPGPKAPAPGQLGEHDRAAHRIPEPQKPHVKPPEDIVRANHYDPEEDEEYYRKQLSYFDRRSFEAKPPTHIPTSHLSEPTRPAHPQSQPQFAGYSSKGKAAEAEAVDRSFGEKRYDPLQATPPPPPLPAQYSQTAQPSPNTALGLHAHTKGAHGEGNAMSLDFQNSLVSKPDPPPSQNKPAAYRPAPREDAVQSAFYPQKSFPEKAPANGVEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPNETAHKPDLSSKAPASPKTLLKSAQPPEFDSGVETFSVHTDNKLKYQVNSVSTGPRAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006066394.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006066394.1

ORF Insert Sequence:

ATGGAACATG CTGCGTATTT TATCTCTCTT CGCCCCCGCC GCCCCATGGT AACGCCGTCC 
TCTGAAAACC CCAGTGGTGC TACTTCTAGT GTCAGCCAAG GAAAGCCTTC TTTAAGACGA 
ATTAAAGGGA GACTGCACAG GAGCAAGAGC CTCGATAGCA TCGATTTCTG TGAACTCACT 
AACACAACAA TGGAGGAAAC AGCTATATGG GAACAACACA CGGTGACACT TCACAGGGCT 
CCTGGATTTG GATTTGGAAT TGCAATATCT GGTGGAAGAG ATAATCCACA TTTCCAGAGC 
GGGGAAACCT CCATCGTGAT TTCAGATGTG CTAAAGGGGG GGCCTGCAGA AGGACAGCTG 
CAGGAAAATG ACCGGGTGGC AATGGTTAAT GGAGTCTCCA TGGATAATGT TGAGCATGCG 
TTTGCTGTTC AGCAACTAAG GAAAAGTGGG AAAAATGCAA AAATTACCAT TCGAAGGAAG 
AAGAAAGTTC AGATCCCAGT AAATCGTCCT GATCCTGAGC CTGTGTCTGA GAACGAAGAG 
GACAGTTACG ACGAGGAGGC GCATGACCCC AGAAGTGGCC GTGGGGCCCT GGCAAACAGG 
AGGAGCGAGA AGAGCTGGGG CCGAGATAGG AGTGCCAGTC GGGAGAGGAG TCTGTCCCCG 
AGGTCTGACA GGCGGTCTGT GGCCTCCAGT CAGCCTGCCA GACCCACCAA AGTCACCCTG 
GTGAAGTCCC GGAAGAATGA AGAATATGGT CTTCGATTGG CCAGCCATAT ATTTGTGAAG 
GAAATTTCAC AAGATAGTTT GGCAGCAAGA GATGGCAATA TTCAAGAAGG CGATGTTGTA 
TTGAAGATAA ATGGTACCGT GACGGAAAAC ATGTCACTGA CAGATGCGAA GACGCTGATC 
GAGAGGTCTA AGGGCAAGTT AAAGATGGTG GTTCAGAGAG ATGAGCGGGC TACCCTGCTG 
AATGTCCCTG ACCTTTCTGA CAGCATCCAC TCTGCTGACG CCTCCGAGAG GGACGATATT 
TCAGAAATCC AGTCACTGGC ATCAGACCAT TCGGGTCGAT CCCACGACCG GCCTCCCCGT 
CACAGCCGCT CTCGGTCTCC TGACCAGCGG TCCGAGCCTT CTGACCATTC TAGACACTCT 
CCACAGCAGC CCAGCAGTGG CAGTCTCCGG AGCAGAGAGG AAGAGAGAAT TTCTAAACCA 
GGGGCTGTCT CGACTCCGGT AAAGCATGCA GATGATCACA CACCTAAAAC AGTGGAAGAA 
GTTGTAGTTG AGCGAAATGA GAAACAAGCA CCCAATCTTC CAGAACCAAA GCCTGTGTAT 
GCCCAGGTAG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG TGTCCTGCCC 
AACTCAACTC ATGAAGATGG CATTCTTCGG CCCAGCATGA AGTTAGTAAA ATTCAGGAAA 
GGAGATAGTG TGGGTCTTCG GCTAGCTGGT GGAAACGATG TTGGCATATT TGTAGCTGGT 
GTTCTCGAAG ACAGCCCTGC TGCCAAAGAA GGCTTGGAGG AGGGCGACCA GATCCTCAGG 
GTAAACAATG TAGATTTCAC AAATATCATA AGAGAAGAAG CCGTCCTCTT CCTGCTTGAC 
CTCCCAAAAG GAGAAGAAGT GACCATATTG GCTCAGAAGA AAAAAGATGT TTATCGTCGC 
ATCGTAGAAT CCGATGTGGG TGATTCTTTC TATATCAGAA CCCATTTTGA ATATGAGAAG 
GAATCTCCCT ATGGACTCAG TTTTAACAAA GGAGAGGTGT TCCGTGTCGT GGACACTCTG 
TACAATGGGA AGCTGGGGTC CTGGCTCGCC ATTCGGATTG GCAAAAACCA TAAAGAGGTG 
GAACGTGGCA TCATCCCAAA CAAAAACAGA GCTGAGCAGT TAGCCAGTGT GCAGTATACA 
CTTCCCAAGA CAGCAGGGGG AGACCGCGCT GACTTCTGGA GGTTCCGCGG TCTGCGCAGC 
TCCAAGAGAA ACCTGCGCAA GAGCCGAGAA GACCTCTCAG CCCAGCCAGT CCAGACCAAG 
TTCCCGGCGT ATGAGAGGGT GGTTCTTCGA GAAGCTGGAT TCCTGAGGCC TGTAACCATC 
TTTGGACCAA TAGCTGATGT TGCCAGGGAG AAGCTGGCAA GAGAGGAGCC AGATATTTAC 
CAGATCGCAA AAAGTGAACC TCGGGATGCA GGAACCGACC AGCGCAGCTC TGGCATTATT 
CGCCTCCACA CAATAAAGCA GATAATAGAT CAGGACAAAC ATGCCTTGCT GGATGTAACG 
CCCAATGCAG TAGACCGTCT GAACTATGCC CAGTGGTATC CAATAGTGGT GTTCCTCAAC 
CCGGACTCAA AGCAGGGTGT GAAAACAATG CGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGCGCCAGGA AGTTATATGA GCGCTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGCTGGT ATGGTGCATT GAAAGAAGCA 
ATCCAGCAAC AACAGAACCA GTTGGTATGG GTTTCTGAGG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAGACCCTAA ATGATGAGGT GGGGACTCCC 
CCGGAGTCTG CCATTACCCG GTCCTCTGAG CCTGTCAGAG AGGACTCCTC TGGAATGCAT 
CCTGACCACC AGGCATATCC TCCCTACTCA CCACAAGCGC AGCCACAGCC AGGCCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGGC 
CCTTACCTTT CGCCTGAAAC AAACCCGGCA CCATCAGCCT CTGCTGTTAA TCCTCACGTA 
GACTTAACTA ATGTCAGACT GGAGGAGCCG AGCCCAGCTC CCCCTTCCTC TGACTCACCA 
CACGCCGACT CTTTACGAGC TCCAGGCACT GAGGCCGCTC ACGTGCAGCT CAGAGGTCCA 
GAGCCGTCCC TGTCGTCGCA TGTGGGCCCA GCAAAGGTGT ACAGGAAGGA CCCCTACCCT 
GAGGAGATGA TGAGACAGAA CCACGTCTTG AAGCAGCCGG CTGCGGTTCA CCCTGGGCAG 
AGGCCAGACA AGGAGCAAGG CCTGAGCTAC GAGCCGCAGC CTCCGTACAC AGAGCGACAG 
GCTGGCAGGG AGTTGGAGCA GCCTGCATAC AGATATGATG CCTCTAGCTA CCCGGAGCAG 
TTTGCTCGGA ACTATGACCA TCGTCTGCGC TATGATGACC GCCTCCTCAC CTACGAGGAG 
CAGTGGCCCT ACTACGACGA CAAGCAGCCT TACCAGCCCC GGCCTCTTGA TGGTCAGCCT 
CCCGCGGAGC CAACAGAACG AGGGTATTTT CCCCGTTTTG AGGAGCCAGT GCCCCTGTCC 
TATGAAGGCC GAGCGCACTA TGACCCACTG CCCAGGACCT CCACCTTGCG GCACGAAGAG 
CCTCCTGCCC CTGCCCCTGC CCCTGGGTAC GACGTGCACG GCAGGTACCG GCCTGACGCG 
CAGCCCTACT CCACTGCTGG CCCCAAGGTG CCCCCTGAGC CCAAGCCGTA CTTGGACCAG 
TACCCTCGGA GTTACGAGCC AGCGCCAGCC CAGGGCTTCC CCTCCAGGGC AGGCCCAGGC 
CACTACGAGC CTCTTCACAG CACCACGCTC GCTCCACCAC TTGGCCCCTC GTCCCAGCAC 
AAGCCCGAAG TCCCGCCCAC AGGCTCCAAA CCCCTGCCTC CACCCCCAGC TCCAGCTGAG 
GAGGAGGAAG ACCCAGCCAT GAAGCCACAA TCCGTGCTCA CCAGAGTGAA GATGTTTGAG 
AACAAGAGGT CTGCTTCCAT GGAGAACAGG AAGGATGAGA GCCATTCCAC TAGTTTCAAG 
CCCTCAGAAG TCACGTCAAA ACCGGCAGGT GCTCCTGTCC CAGGTCCCAA AGCCCCTGCT 
CCGGGTCAGC TCGGTGAACA CGACAGAGCA GCGCATAGGA TTCCTGAACC ACAGAAGCCT 
CACGTGAAGC CACCTGAAGA CATTGTTCGG GCAAATCACT ATGACCCCGA GGAGGATGAG 
GAGTACTACC GGAAGCAGCT CTCCTACTTC GATCGGAGAA GCTTTGAGGC CAAGCCCCCC 
ACCCACATCC CCACTAGCCA CCTCTCCGAG CCCACCAGGC CGGCTCACCC CCAATCTCAG 
CCACAGTTTG CCGGTTACTC TTCCAAGGGG AAGGCTGCTG AGGCCGAAGC TGTGGACAGG 
TCATTCGGGG AGAAGCGCTA TGACCCGCTG CAGGCGACGC CCCCCCCTCC GCCGCTGCCC 
GCTCAGTACA GCCAGACTGC TCAACCCAGC CCCAACACGG CTCTCGGCCT CCACGCACAC 
ACCAAGGGGG CGCACGGTGA AGGTAATGCC ATGTCATTGG ATTTTCAGAA CTCGTTAGTG 
TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAG CTTACAGACC AGCCCCTCGA 
GAGGATGCTG TCCAGTCTGC CTTCTACCCA CAAAAGAGTT TCCCAGAGAA AGCCCCAGCT 
AATGGGGTCG AGCAGACGCA GAAAACCGTC ACGCCAGCAT ACAATCGATT CACACCAAAA 
CCATACACAA GCTCTGCACG GCCGTTTGAA CGCAAGTTTG AAAGCCCTAA ATTCAACCAC 
AATCTCTTGC CGAATGAAAC CGCACACAAA CCTGACCTGT CCTCCAAAGC CCCTGCTTCT 
CCGAAAACCC TCCTGAAGTC TGCACAGCCT CCTGAGTTTG ACAGTGGTGT GGAGACCTTC 
TCTGTCCACA CAGACAACAA GCTGAAATAC CAAGTAAACA GTGTTAGCAC GGGGCCGAGA 
GCAGTGCCCG TGAGTCCCTC AGCCGTGGAG GAAGATGAAG ATGAAGACGG TCACACAGTG 
GTGGCTACTG CCCGCGGCGT GTTTAACAGC AATGGTGGGG TGCTGAGCTC CATTGAGACC 
GGTGTCAGCA TCATCATCCC CCAGGGAGCC ATTCCTGAGG GGGTGGAACA GGAGATCTAC 
TTCAAGGTCT GCCGCGACAA CAGCATCCTG CCGCCTTTGG ACAAAGAGAA GGGTGAGACC 
CTGCTGAGCC CCCTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCCTAC CACACTGTGC GTCCATGACT CCTGACGGTG ATCCTAAAAC CTGGCAAAAC 
AAGTGTCTTC CTGGAGATCC AAATTATCTT GTTGGAGCAA ACTGTGTTTC TGTCCTGATT 
GACCACTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_006066395.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006066457.1 Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu21757
Clone ID Related Accession (Same CDS sequence)	XM_006066395.1
Accession Version	XM_006066395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	tight junction protein ZO-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785206.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACATG CTGCGTATTT TATCTCTCTT CGCCCCCGCC GCCCCATGGT AACGCCGTCC 
TCTGAAAACC CCAGTGGTGC TACTTCTAGT GTCAGCCAAG GAAAGCCTTC TTTAAGACGA 
ATTAAAGGGA GACTGCACAG GAGCAAGAGC CTCGATAGCA TCGATTTCTG TGAACTCACT 
AACACAACAA TGGAGGAAAC AGCTATATGG GAACAACACA CGGTGACACT TCACAGGGCT 
CCTGGATTTG GATTTGGAAT TGCAATATCT GGTGGAAGAG ATAATCCACA TTTCCAGAGC 
GGGGAAACCT CCATCGTGAT TTCAGATGTG CTAAAGGGGG GGCCTGCAGA AGGACAGCTG 
CAGGAAAATG ACCGGGTGGC AATGGTTAAT GGAGTCTCCA TGGATAATGT TGAGCATGCG 
TTTGCTGTTC AGCAACTAAG GAAAAGTGGG AAAAATGCAA AAATTACCAT TCGAAGGAAG 
AAGAAAGTTC AGATCCCAGT AAATCGTCCT GATCCTGAGC CTGTGTCTGA GAACGAAGAG 
GACAGTTACG ACGAGGAGGC GCATGACCCC AGAAGTGGCC GTGGGGCCCT GGCAAACAGG 
AGGAGCGAGA AGAGCTGGGG CCGAGATAGG AGTGCCAGTC GGGAGAGGAG TCTGTCCCCG 
AGGTCTGACA GGCGGTCTGT GGCCTCCAGT CAGCCTGCCA GACCCACCAA AGTCACCCTG 
GTGAAGTCCC GGAAGAATGA AGAATATGGT CTTCGATTGG CCAGCCATAT ATTTGTGAAG 
GAAATTTCAC AAGATAGTTT GGCAGCAAGA GATGGCAATA TTCAAGAAGG CGATGTTGTA 
TTGAAGATAA ATGGTACCGT GACGGAAAAC ATGTCACTGA CAGATGCGAA GACGCTGATC 
GAGAGGTCTA AGGGCAAGTT AAAGATGGTG GTTCAGAGAG ATGAGCGGGC TACCCTGCTG 
AATGTCCCTG ACCTTTCTGA CAGCATCCAC TCTGCTGACG CCTCCGAGAG GGACGATATT 
TCAGAAATCC AGTCACTGGC ATCAGACCAT TCGGGTCGAT CCCACGACCG GCCTCCCCGT 
CACAGCCGCT CTCGGTCTCC TGACCAGCGG TCCGAGCCTT CTGACCATTC TAGACACTCT 
CCACAGCAGC CCAGCAGTGG CAGTCTCCGG AGCAGAGAGG AAGAGAGAAT TTCTAAACCA 
GGGGCTGTCT CGACTCCGGT AAAGCATGCA GATGATCACA CACCTAAAAC AGTGGAAGAA 
GTTGTAGTTG AGCGAAATGA GAAACAAGCA CCCAATCTTC CAGAACCAAA GCCTGTGTAT 
GCCCAGGTAG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG TGTCCTGCCC 
AACTCAACTC ATGAAGATGG CATTCTTCGG CCCAGCATGA AGTTAGTAAA ATTCAGGAAA 
GGAGATAGTG TGGGTCTTCG GCTAGCTGGT GGAAACGATG TTGGCATATT TGTAGCTGGT 
GTTCTCGAAG ACAGCCCTGC TGCCAAAGAA GGCTTGGAGG AGGGCGACCA GATCCTCAGG 
GTAAACAATG TAGATTTCAC AAATATCATA AGAGAAGAAG CCGTCCTCTT CCTGCTTGAC 
CTCCCAAAAG GAGAAGAAGT GACCATATTG GCTCAGAAGA AAAAAGATGT TTATCGTCGC 
ATCGTAGAAT CCGATGTGGG TGATTCTTTC TATATCAGAA CCCATTTTGA ATATGAGAAG 
GAATCTCCCT ATGGACTCAG TTTTAACAAA GGAGAGGTGT TCCGTGTCGT GGACACTCTG 
TACAATGGGA AGCTGGGGTC CTGGCTCGCC ATTCGGATTG GCAAAAACCA TAAAGAGGTG 
GAACGTGGCA TCATCCCAAA CAAAAACAGA GCTGAGCAGT TAGCCAGTGT GCAGTATACA 
CTTCCCAAGA CAGCAGGGGG AGACCGCGCT GACTTCTGGA GGTTCCGCGG TCTGCGCAGC 
TCCAAGAGAA ACCTGCGCAA GAGCCGAGAA GACCTCTCAG CCCAGCCAGT CCAGACCAAG 
TTCCCGGCGT ATGAGAGGGT GGTTCTTCGA GAAGCTGGAT TCCTGAGGCC TGTAACCATC 
TTTGGACCAA TAGCTGATGT TGCCAGGGAG AAGCTGGCAA GAGAGGAGCC AGATATTTAC 
CAGATCGCAA AAAGTGAACC TCGGGATGCA GGAACCGACC AGCGCAGCTC TGGCATTATT 
CGCCTCCACA CAATAAAGCA GATAATAGAT CAGGACAAAC ATGCCTTGCT GGATGTAACG 
CCCAATGCAG TAGACCGTCT GAACTATGCC CAGTGGTATC CAATAGTGGT GTTCCTCAAC 
CCGGACTCAA AGCAGGGTGT GAAAACAATG CGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGCGCCAGGA AGTTATATGA GCGCTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGCTGGT ATGGTGCATT GAAAGAAGCA 
ATCCAGCAAC AACAGAACCA GTTGGTATGG GTTTCTGAGG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAGACCCTAA ATGATGAGGT GGGGACTCCC 
CCGGAGTCTG CCATTACCCG GTCCTCTGAG CCTGTCAGAG AGGACTCCTC TGGAATGCAT 
CCTGACCACC AGGCATATCC TCCCTACTCA CCACAAGCGC AGCCACAGCC AGGCCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGGC 
CCTTACCTTT CGCCTGAAAC AAACCCGGCA CCATCAGCCT CTGCTGTTAA TCCTCACGTA 
GACTTAACTA ATGTCAGACT GGAGGAGCCG AGCCCAGCTC CCCCTTCCTC TGACTCACCA 
CACGCCGACT CTTTACGAGC TCCAGGCACT GAGGCCGCTC ACGTGCAGCT CAGAGGTCCA 
GAGCCGTCCC TGTCGTCGCA TGTGGGCCCA GCAAAGGTGT ACAGGAAGGA CCCCTACCCT 
GAGGAGATGA TGAGACAGAA CCACGTCTTG AAGCAGCCGG CTGCGGTTCA CCCTGGGCAG 
AGGCCAGACA AGGAGCAAGG CCTGAGCTAC GAGCCGCAGC CTCCGTACAC AGAGCGACAG 
GCTGGCAGGG AGTTGGAGCA GCCTGCATAC AGATATGATG CCTCTAGCTA CCCGGAGCAG 
TTTGCTCGGA ACTATGACCA TCGTCTGCGC TATGATGACC GCCTCCTCAC CTACGAGGAG 
CAGTGGCCCT ACTACGACGA CAAGCAGCCT TACCAGCCCC GGCCTCTTGA TGGTCAGCCT 
CCCGCGGAGC CAACAGAACG AGGGTATTTT CCCCGTTTTG AGGAGCCAGT GCCCCTGTCC 
TATGAAGGCC GAGCGCACTA TGACCCACTG CCCAGGACCT CCACCTTGCG GCACGAAGAG 
CCTCCTGCCC CTGCCCCTGC CCCTGGGTAC GACGTGCACG GCAGGTACCG GCCTGACGCG 
CAGCCCTACT CCACTGCTGG CCCCAAGGTG CCCCCTGAGC CCAAGCCGTA CTTGGACCAG 
TACCCTCGGA GTTACGAGCC AGCGCCAGCC CAGGGCTTCC CCTCCAGGGC AGGCCCAGGC 
CACTACGAGC CTCTTCACAG CACCACGCTC GCTCCACCAC TTGGCCCCTC GTCCCAGCAC 
AAGCCCGAAG TCCCGCCCAC AGGCTCCAAA CCCCTGCCTC CACCCCCAGC TCCAGCTGAG 
GAGGAGGAAG ACCCAGCCAT GAAGCCACAA TCCGTGCTCA CCAGAGTGAA GATGTTTGAG 
AACAAGAGGT CTGCTTCCAT GGAGAACAGG AAGGATGAGA GCCATTCCAC TAGTTTCAAG 
CCCTCAGAAG TCACGTCAAA ACCGGCAGGT GCTCCTGTCC CAGGTCCCAA AGCCCCTGCT 
CCGGGTCAGC TCGGTGAACA CGACAGAGCA GCGCATAGGA TTCCTGAACC ACAGAAGCCT 
CACGTGAAGC CACCTGAAGA CATTGTTCGG GCAAATCACT ATGACCCCGA GGAGGATGAG 
GAGTACTACC GGAAGCAGCT CTCCTACTTC GATCGGAGAA GCTTTGAGGC CAAGCCCCCC 
ACCCACATCC CCACTAGCCA CCTCTCCGAG CCCACCAGGC CGGCTCACCC CCAATCTCAG 
CCACAGTTTG CCGGTTACTC TTCCAAGGGG AAGGCTGCTG AGGCCGAAGC TGTGGACAGG 
TCATTCGGGG AGAAGCGCTA TGACCCGCTG CAGGCGACGC CCCCCCCTCC GCCGCTGCCC 
GCTCAGTACA GCCAGACTGC TCAACCCAGC CCCAACACGG CTCTCGGCCT CCACGCACAC 
ACCAAGGGGG CGCACGGTGA AGGTAATGCC ATGTCATTGG ATTTTCAGAA CTCGTTAGTG 
TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAG CTTACAGACC AGCCCCTCGA 
GAGGATGCTG TCCAGTCTGC CTTCTACCCA CAAAAGAGTT TCCCAGAGAA AGCCCCAGCT 
AATGGGGTCG AGCAGACGCA GAAAACCGTC ACGCCAGCAT ACAATCGATT CACACCAAAA 
CCATACACAA GCTCTGCACG GCCGTTTGAA CGCAAGTTTG AAAGCCCTAA ATTCAACCAC 
AATCTCTTGC CGAATGAAAC CGCACACAAA CCTGACCTGT CCTCCAAAGC CCCTGCTTCT 
CCGAAAACCC TCCTGAAGTC TGCACAGCCT CCTGAGTTTG ACAGTGGTGT GGAGACCTTC 
TCTGTCCACA CAGACAACAA GCTGAAATAC CAAGTAAACA GTGTTAGCAC GGGGCCGAGA 
GCAGTGCCCG TGAGTCCCTC AGCCGTGGAG GAAGATGAAG ATGAAGACGG TCACACAGTG 
GTGGCTACTG CCCGCGGCGT GTTTAACAGC AATGGTGGGG TGCTGAGCTC CATTGAGACC 
GGTGTCAGCA TCATCATCCC CCAGGGAGCC ATTCCTGAGG GGGTGGAACA GGAGATCTAC 
TTCAAGGTCT GCCGCGACAA CAGCATCCTG CCGCCTTTGG ACAAAGAGAA GGGTGAGACC 
CTGCTGAGCC CCCTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCCTAC CACACTGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTATCTTG TTGGAGCAAA CTGTGTTTCT GTCCTGATTG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006066457.1
CDS	1..5391
Translation	MEHAAYFISLRPRRPMVTPSSENPSGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTTMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVNRPDPEPVSENEEDSYDEEAHDPRSGRGALANRRSEKSWGRDRSASRERSLSPRSDRRSVASSQPARPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSADASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTVEEVVVERNEKQAPNLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHPDHQAYPPYSPQAQPQPGHRIDSPGFKTASQQKAEASSPGPYLSPETNPAPSASAVNPHVDLTNVRLEEPSPAPPSSDSPHADSLRAPGTEAAHVQLRGPEPSLSSHVGPAKVYRKDPYPEEMMRQNHVLKQPAAVHPGQRPDKEQGLSYEPQPPYTERQAGRELEQPAYRYDASSYPEQFARNYDHRLRYDDRLLTYEEQWPYYDDKQPYQPRPLDGQPPAEPTERGYFPRFEEPVPLSYEGRAHYDPLPRTSTLRHEEPPAPAPAPGYDVHGRYRPDAQPYSTAGPKVPPEPKPYLDQYPRSYEPAPAQGFPSRAGPGHYEPLHSTTLAPPLGPSSQHKPEVPPTGSKPLPPPPAPAEEEEDPAMKPQSVLTRVKMFENKRSASMENRKDESHSTSFKPSEVTSKPAGAPVPGPKAPAPGQLGEHDRAAHRIPEPQKPHVKPPEDIVRANHYDPEEDEEYYRKQLSYFDRRSFEAKPPTHIPTSHLSEPTRPAHPQSQPQFAGYSSKGKAAEAEAVDRSFGEKRYDPLQATPPPPPLPAQYSQTAQPSPNTALGLHAHTKGAHGEGNAMSLDFQNSLVSKPDPPPSQNKPAAYRPAPREDAVQSAFYPQKSFPEKAPANGVEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPNETAHKPDLSSKAPASPKTLLKSAQPPEFDSGVETFSVHTDNKLKYQVNSVSTGPRAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006066395.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006066395.1

ORF Insert Sequence:

ATGGAACATG CTGCGTATTT TATCTCTCTT CGCCCCCGCC GCCCCATGGT AACGCCGTCC 
TCTGAAAACC CCAGTGGTGC TACTTCTAGT GTCAGCCAAG GAAAGCCTTC TTTAAGACGA 
ATTAAAGGGA GACTGCACAG GAGCAAGAGC CTCGATAGCA TCGATTTCTG TGAACTCACT 
AACACAACAA TGGAGGAAAC AGCTATATGG GAACAACACA CGGTGACACT TCACAGGGCT 
CCTGGATTTG GATTTGGAAT TGCAATATCT GGTGGAAGAG ATAATCCACA TTTCCAGAGC 
GGGGAAACCT CCATCGTGAT TTCAGATGTG CTAAAGGGGG GGCCTGCAGA AGGACAGCTG 
CAGGAAAATG ACCGGGTGGC AATGGTTAAT GGAGTCTCCA TGGATAATGT TGAGCATGCG 
TTTGCTGTTC AGCAACTAAG GAAAAGTGGG AAAAATGCAA AAATTACCAT TCGAAGGAAG 
AAGAAAGTTC AGATCCCAGT AAATCGTCCT GATCCTGAGC CTGTGTCTGA GAACGAAGAG 
GACAGTTACG ACGAGGAGGC GCATGACCCC AGAAGTGGCC GTGGGGCCCT GGCAAACAGG 
AGGAGCGAGA AGAGCTGGGG CCGAGATAGG AGTGCCAGTC GGGAGAGGAG TCTGTCCCCG 
AGGTCTGACA GGCGGTCTGT GGCCTCCAGT CAGCCTGCCA GACCCACCAA AGTCACCCTG 
GTGAAGTCCC GGAAGAATGA AGAATATGGT CTTCGATTGG CCAGCCATAT ATTTGTGAAG 
GAAATTTCAC AAGATAGTTT GGCAGCAAGA GATGGCAATA TTCAAGAAGG CGATGTTGTA 
TTGAAGATAA ATGGTACCGT GACGGAAAAC ATGTCACTGA CAGATGCGAA GACGCTGATC 
GAGAGGTCTA AGGGCAAGTT AAAGATGGTG GTTCAGAGAG ATGAGCGGGC TACCCTGCTG 
AATGTCCCTG ACCTTTCTGA CAGCATCCAC TCTGCTGACG CCTCCGAGAG GGACGATATT 
TCAGAAATCC AGTCACTGGC ATCAGACCAT TCGGGTCGAT CCCACGACCG GCCTCCCCGT 
CACAGCCGCT CTCGGTCTCC TGACCAGCGG TCCGAGCCTT CTGACCATTC TAGACACTCT 
CCACAGCAGC CCAGCAGTGG CAGTCTCCGG AGCAGAGAGG AAGAGAGAAT TTCTAAACCA 
GGGGCTGTCT CGACTCCGGT AAAGCATGCA GATGATCACA CACCTAAAAC AGTGGAAGAA 
GTTGTAGTTG AGCGAAATGA GAAACAAGCA CCCAATCTTC CAGAACCAAA GCCTGTGTAT 
GCCCAGGTAG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG TGTCCTGCCC 
AACTCAACTC ATGAAGATGG CATTCTTCGG CCCAGCATGA AGTTAGTAAA ATTCAGGAAA 
GGAGATAGTG TGGGTCTTCG GCTAGCTGGT GGAAACGATG TTGGCATATT TGTAGCTGGT 
GTTCTCGAAG ACAGCCCTGC TGCCAAAGAA GGCTTGGAGG AGGGCGACCA GATCCTCAGG 
GTAAACAATG TAGATTTCAC AAATATCATA AGAGAAGAAG CCGTCCTCTT CCTGCTTGAC 
CTCCCAAAAG GAGAAGAAGT GACCATATTG GCTCAGAAGA AAAAAGATGT TTATCGTCGC 
ATCGTAGAAT CCGATGTGGG TGATTCTTTC TATATCAGAA CCCATTTTGA ATATGAGAAG 
GAATCTCCCT ATGGACTCAG TTTTAACAAA GGAGAGGTGT TCCGTGTCGT GGACACTCTG 
TACAATGGGA AGCTGGGGTC CTGGCTCGCC ATTCGGATTG GCAAAAACCA TAAAGAGGTG 
GAACGTGGCA TCATCCCAAA CAAAAACAGA GCTGAGCAGT TAGCCAGTGT GCAGTATACA 
CTTCCCAAGA CAGCAGGGGG AGACCGCGCT GACTTCTGGA GGTTCCGCGG TCTGCGCAGC 
TCCAAGAGAA ACCTGCGCAA GAGCCGAGAA GACCTCTCAG CCCAGCCAGT CCAGACCAAG 
TTCCCGGCGT ATGAGAGGGT GGTTCTTCGA GAAGCTGGAT TCCTGAGGCC TGTAACCATC 
TTTGGACCAA TAGCTGATGT TGCCAGGGAG AAGCTGGCAA GAGAGGAGCC AGATATTTAC 
CAGATCGCAA AAAGTGAACC TCGGGATGCA GGAACCGACC AGCGCAGCTC TGGCATTATT 
CGCCTCCACA CAATAAAGCA GATAATAGAT CAGGACAAAC ATGCCTTGCT GGATGTAACG 
CCCAATGCAG TAGACCGTCT GAACTATGCC CAGTGGTATC CAATAGTGGT GTTCCTCAAC 
CCGGACTCAA AGCAGGGTGT GAAAACAATG CGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGCGCCAGGA AGTTATATGA GCGCTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGCTGGT ATGGTGCATT GAAAGAAGCA 
ATCCAGCAAC AACAGAACCA GTTGGTATGG GTTTCTGAGG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAGACCCTAA ATGATGAGGT GGGGACTCCC 
CCGGAGTCTG CCATTACCCG GTCCTCTGAG CCTGTCAGAG AGGACTCCTC TGGAATGCAT 
CCTGACCACC AGGCATATCC TCCCTACTCA CCACAAGCGC AGCCACAGCC AGGCCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGAAAG CAGAAGCCTC ATCTCCAGGC 
CCTTACCTTT CGCCTGAAAC AAACCCGGCA CCATCAGCCT CTGCTGTTAA TCCTCACGTA 
GACTTAACTA ATGTCAGACT GGAGGAGCCG AGCCCAGCTC CCCCTTCCTC TGACTCACCA 
CACGCCGACT CTTTACGAGC TCCAGGCACT GAGGCCGCTC ACGTGCAGCT CAGAGGTCCA 
GAGCCGTCCC TGTCGTCGCA TGTGGGCCCA GCAAAGGTGT ACAGGAAGGA CCCCTACCCT 
GAGGAGATGA TGAGACAGAA CCACGTCTTG AAGCAGCCGG CTGCGGTTCA CCCTGGGCAG 
AGGCCAGACA AGGAGCAAGG CCTGAGCTAC GAGCCGCAGC CTCCGTACAC AGAGCGACAG 
GCTGGCAGGG AGTTGGAGCA GCCTGCATAC AGATATGATG CCTCTAGCTA CCCGGAGCAG 
TTTGCTCGGA ACTATGACCA TCGTCTGCGC TATGATGACC GCCTCCTCAC CTACGAGGAG 
CAGTGGCCCT ACTACGACGA CAAGCAGCCT TACCAGCCCC GGCCTCTTGA TGGTCAGCCT 
CCCGCGGAGC CAACAGAACG AGGGTATTTT CCCCGTTTTG AGGAGCCAGT GCCCCTGTCC 
TATGAAGGCC GAGCGCACTA TGACCCACTG CCCAGGACCT CCACCTTGCG GCACGAAGAG 
CCTCCTGCCC CTGCCCCTGC CCCTGGGTAC GACGTGCACG GCAGGTACCG GCCTGACGCG 
CAGCCCTACT CCACTGCTGG CCCCAAGGTG CCCCCTGAGC CCAAGCCGTA CTTGGACCAG 
TACCCTCGGA GTTACGAGCC AGCGCCAGCC CAGGGCTTCC CCTCCAGGGC AGGCCCAGGC 
CACTACGAGC CTCTTCACAG CACCACGCTC GCTCCACCAC TTGGCCCCTC GTCCCAGCAC 
AAGCCCGAAG TCCCGCCCAC AGGCTCCAAA CCCCTGCCTC CACCCCCAGC TCCAGCTGAG 
GAGGAGGAAG ACCCAGCCAT GAAGCCACAA TCCGTGCTCA CCAGAGTGAA GATGTTTGAG 
AACAAGAGGT CTGCTTCCAT GGAGAACAGG AAGGATGAGA GCCATTCCAC TAGTTTCAAG 
CCCTCAGAAG TCACGTCAAA ACCGGCAGGT GCTCCTGTCC CAGGTCCCAA AGCCCCTGCT 
CCGGGTCAGC TCGGTGAACA CGACAGAGCA GCGCATAGGA TTCCTGAACC ACAGAAGCCT 
CACGTGAAGC CACCTGAAGA CATTGTTCGG GCAAATCACT ATGACCCCGA GGAGGATGAG 
GAGTACTACC GGAAGCAGCT CTCCTACTTC GATCGGAGAA GCTTTGAGGC CAAGCCCCCC 
ACCCACATCC CCACTAGCCA CCTCTCCGAG CCCACCAGGC CGGCTCACCC CCAATCTCAG 
CCACAGTTTG CCGGTTACTC TTCCAAGGGG AAGGCTGCTG AGGCCGAAGC TGTGGACAGG 
TCATTCGGGG AGAAGCGCTA TGACCCGCTG CAGGCGACGC CCCCCCCTCC GCCGCTGCCC 
GCTCAGTACA GCCAGACTGC TCAACCCAGC CCCAACACGG CTCTCGGCCT CCACGCACAC 
ACCAAGGGGG CGCACGGTGA AGGTAATGCC ATGTCATTGG ATTTTCAGAA CTCGTTAGTG 
TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAG CTTACAGACC AGCCCCTCGA 
GAGGATGCTG TCCAGTCTGC CTTCTACCCA CAAAAGAGTT TCCCAGAGAA AGCCCCAGCT 
AATGGGGTCG AGCAGACGCA GAAAACCGTC ACGCCAGCAT ACAATCGATT CACACCAAAA 
CCATACACAA GCTCTGCACG GCCGTTTGAA CGCAAGTTTG AAAGCCCTAA ATTCAACCAC 
AATCTCTTGC CGAATGAAAC CGCACACAAA CCTGACCTGT CCTCCAAAGC CCCTGCTTCT 
CCGAAAACCC TCCTGAAGTC TGCACAGCCT CCTGAGTTTG ACAGTGGTGT GGAGACCTTC 
TCTGTCCACA CAGACAACAA GCTGAAATAC CAAGTAAACA GTGTTAGCAC GGGGCCGAGA 
GCAGTGCCCG TGAGTCCCTC AGCCGTGGAG GAAGATGAAG ATGAAGACGG TCACACAGTG 
GTGGCTACTG CCCGCGGCGT GTTTAACAGC AATGGTGGGG TGCTGAGCTC CATTGAGACC 
GGTGTCAGCA TCATCATCCC CCAGGGAGCC ATTCCTGAGG GGGTGGAACA GGAGATCTAC 
TTCAAGGTCT GCCGCGACAA CAGCATCCTG CCGCCTTTGG ACAAAGAGAA GGGTGAGACC 
CTGCTGAGCC CCCTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCCTAC CACACTGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTATCTTG TTGGAGCAAA CTGTGTTTCT GTCCTGATTG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_006066396.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006066458.1 Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X4, mRNA.

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CloneID	OBu21758
Clone ID Related Accession (Same CDS sequence)	XM_006066396.1 , XM_006066396.2
Accession Version	XM_006066396.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	tight junction protein ZO-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785206.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAACAATGG AGGAAACAGC TATATGGGAA 
CAACACACGG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ATCCACATTT CCAGAGCGGG GAAACCTCCA TCGTGATTTC AGATGTGCTA 
AAGGGGGGGC CTGCAGAAGG ACAGCTGCAG GAAAATGACC GGGTGGCAAT GGTTAATGGA 
GTCTCCATGG ATAATGTTGA GCATGCGTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACCATTCG AAGGAAGAAG AAAGTTCAGA TCCCAGTAAA TCGTCCTGAT 
CCTGAGCCTG TGTCTGAGAA CGAAGAGGAC AGTTACGACG AGGAGGCGCA TGACCCCAGA 
AGTGGCCGTG GGGCCCTGGC AAACAGGAGG AGCGAGAAGA GCTGGGGCCG AGATAGGAGT 
GCCAGTCGGG AGAGGAGTCT GTCCCCGAGG TCTGACAGGC GGTCTGTGGC CTCCAGTCAG 
CCTGCCAGAC CCACCAAAGT CACCCTGGTG AAGTCCCGGA AGAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTGAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC GGAAAACATG 
TCACTGACAG ATGCGAAGAC GCTGATCGAG AGGTCTAAGG GCAAGTTAAA GATGGTGGTT 
CAGAGAGATG AGCGGGCTAC CCTGCTGAAT GTCCCTGACC TTTCTGACAG CATCCACTCT 
GCTGACGCCT CCGAGAGGGA CGATATTTCA GAAATCCAGT CACTGGCATC AGACCATTCG 
GGTCGATCCC ACGACCGGCC TCCCCGTCAC AGCCGCTCTC GGTCTCCTGA CCAGCGGTCC 
GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAGTGGCAG TCTCCGGAGC 
AGAGAGGAAG AGAGAATTTC TAAACCAGGG GCTGTCTCGA CTCCGGTAAA GCATGCAGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT GTAGTTGAGC GAAATGAGAA ACAAGCACCC 
AATCTTCCAG AACCAAAGCC TGTGTATGCC CAGGTAGGAC AGCCAGACGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTGCCCAAC TCAACTCATG AAGATGGCAT TCTTCGGCCC 
AGCATGAAGT TAGTAAAATT CAGGAAAGGA GATAGTGTGG GTCTTCGGCT AGCTGGTGGA 
AACGATGTTG GCATATTTGT AGCTGGTGTT CTCGAAGACA GCCCTGCTGC CAAAGAAGGC 
TTGGAGGAGG GCGACCAGAT CCTCAGGGTA AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAAGCCG TCCTCTTCCT GCTTGACCTC CCAAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAAA AAGATGTTTA TCGTCGCATC GTAGAATCCG ATGTGGGTGA TTCTTTCTAT 
ATCAGAACCC ATTTTGAATA TGAGAAGGAA TCTCCCTATG GACTCAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTCGTGGA CACTCTGTAC AATGGGAAGC TGGGGTCCTG GCTCGCCATT 
CGGATTGGCA AAAACCATAA AGAGGTGGAA CGTGGCATCA TCCCAAACAA AAACAGAGCT 
GAGCAGTTAG CCAGTGTGCA GTATACACTT CCCAAGACAG CAGGGGGAGA CCGCGCTGAC 
TTCTGGAGGT TCCGCGGTCT GCGCAGCTCC AAGAGAAACC TGCGCAAGAG CCGAGAAGAC 
CTCTCAGCCC AGCCAGTCCA GACCAAGTTC CCGGCGTATG AGAGGGTGGT TCTTCGAGAA 
GCTGGATTCC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGGGAGAAG 
CTGGCAAGAG AGGAGCCAGA TATTTACCAG ATCGCAAAAA GTGAACCTCG GGATGCAGGA 
ACCGACCAGC GCAGCTCTGG CATTATTCGC CTCCACACAA TAAAGCAGAT AATAGATCAG 
GACAAACATG CCTTGCTGGA TGTAACGCCC AATGCAGTAG ACCGTCTGAA CTATGCCCAG 
TGGTATCCAA TAGTGGTGTT CCTCAACCCG GACTCAAAGC AGGGTGTGAA AACAATGCGG 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGC GCCAGGAAGT TATATGAGCG CTCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGCTGGTATG GTGCATTGAA AGAAGCAATC CAGCAACAAC AGAACCAGTT GGTATGGGTT 
TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAG 
ACCCTAAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACCCGGTC CTCTGAGCCT 
GTCAGAGAGG ACTCCTCTGG AATGCATCCT GACCACCAGG CATATCCTCC CTACTCACCA 
CAAGCGCAGC CACAGCCAGG CCATAGAATA GACTCCCCTG GATTTAAAAC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGGCCCT TACCTTTCGC CTGAAACAAA CCCGGCACCA 
TCAGCCTCTG CTGTTAATCC TCACGTAGAC TTAACTAATG TCAGACTGGA GGAGCCGAGC 
CCAGCTCCCC CTTCCTCTGA CTCACCACAC GCCGACTCTT TACGAGCTCC AGGCACTGAG 
GCCGCTCACG TGCAGCTCAG AGGTCCAGAG CCGTCCCTGT CGTCGCATGT GGGCCCAGCA 
AAGGTGTACA GGAAGGACCC CTACCCTGAG GAGATGATGA GACAGAACCA CGTCTTGAAG 
CAGCCGGCTG CGGTTCACCC TGGGCAGAGG CCAGACAAGG AGCAAGGCCT GAGCTACGAG 
CCGCAGCCTC CGTACACAGA GCGACAGGCT GGCAGGGAGT TGGAGCAGCC TGCATACAGA 
TATGATGCCT CTAGCTACCC GGAGCAGTTT GCTCGGAACT ATGACCATCG TCTGCGCTAT 
GATGACCGCC TCCTCACCTA CGAGGAGCAG TGGCCCTACT ACGACGACAA GCAGCCTTAC 
CAGCCCCGGC CTCTTGATGG TCAGCCTCCC GCGGAGCCAA CAGAACGAGG GTATTTTCCC 
CGTTTTGAGG AGCCAGTGCC CCTGTCCTAT GAAGGCCGAG CGCACTATGA CCCACTGCCC 
AGGACCTCCA CCTTGCGGCA CGAAGAGCCT CCTGCCCCTG CCCCTGCCCC TGGGTACGAC 
GTGCACGGCA GGTACCGGCC TGACGCGCAG CCCTACTCCA CTGCTGGCCC CAAGGTGCCC 
CCTGAGCCCA AGCCGTACTT GGACCAGTAC CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG 
GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC TACGAGCCTC TTCACAGCAC CACGCTCGCT 
CCACCACTTG GCCCCTCGTC CCAGCACAAG CCCGAAGTCC CGCCCACAGG CTCCAAACCC 
CTGCCTCCAC CCCCAGCTCC AGCTGAGGAG GAGGAAGACC CAGCCATGAA GCCACAATCC 
GTGCTCACCA GAGTGAAGAT GTTTGAGAAC AAGAGGTCTG CTTCCATGGA GAACAGGAAG 
GATGAGAGCC ATTCCACTAG TTTCAAGCCC TCAGAAGTCA CGTCAAAACC GGCAGGTGCT 
CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG GGTCAGCTCG GTGAACACGA CAGAGCAGCG 
CATAGGATTC CTGAACCACA GAAGCCTCAC GTGAAGCCAC CTGAAGACAT TGTTCGGGCA 
AATCACTATG ACCCCGAGGA GGATGAGGAG TACTACCGGA AGCAGCTCTC CTACTTCGAT 
CGGAGAAGCT TTGAGGCCAA GCCCCCCACC CACATCCCCA CTAGCCACCT CTCCGAGCCC 
ACCAGGCCGG CTCACCCCCA ATCTCAGCCA CAGTTTGCCG GTTACTCTTC CAAGGGGAAG 
GCTGCTGAGG CCGAAGCTGT GGACAGGTCA TTCGGGGAGA AGCGCTATGA CCCGCTGCAG 
GCGACGCCCC CCCCTCCGCC GCTGCCCGCT CAGTACAGCC AGACTGCTCA ACCCAGCCCC 
AACACGGCTC TCGGCCTCCA CGCACACACC AAGGGGGCGC ACGGTGAAGG TAATGCCATG 
TCATTGGATT TTCAGAACTC GTTAGTGTCC AAACCAGACC CACCTCCATC TCAGAATAAG 
CCAGCAGCTT ACAGACCAGC CCCTCGAGAG GATGCTGTCC AGTCTGCCTT CTACCCACAA 
AAGAGTTTCC CAGAGAAAGC CCCAGCTAAT GGGGTCGAGC AGACGCAGAA AACCGTCACG 
CCAGCATACA ATCGATTCAC ACCAAAACCA TACACAAGCT CTGCACGGCC GTTTGAACGC 
AAGTTTGAAA GCCCTAAATT CAACCACAAT CTCTTGCCGA ATGAAACCGC ACACAAACCT 
GACCTGTCCT CCAAAGCCCC TGCTTCTCCG AAAACCCTCC TGAAGTCTGC ACAGCCTCCT 
GAGTTTGACA GTGGTGTGGA GACCTTCTCT GTCCACACAG ACAACAAGCT GAAATACCAA 
GTAAACAGTG TTAGCACGGG GCCGAGAGCA GTGCCCGTGA GTCCCTCAGC CGTGGAGGAA 
GATGAAGATG AAGACGGTCA CACAGTGGTG GCTACTGCCC GCGGCGTGTT TAACAGCAAT 
GGTGGGGTGC TGAGCTCCAT TGAGACCGGT GTCAGCATCA TCATCCCCCA GGGAGCCATT 
CCTGAGGGGG TGGAACAGGA GATCTACTTC AAGGTCTGCC GCGACAACAG CATCCTGCCG 
CCTTTGGACA AAGAGAAGGG TGAGACCCTG CTGAGCCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCCTACCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCAAAT TATCTTGTTG GAGCAAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006066458.1
CDS	32..5329
Translation	MSARAAAAKNTTMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVNRPDPEPVSENEEDSYDEEAHDPRSGRGALANRRSEKSWGRDRSASRERSLSPRSDRRSVASSQPARPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSADASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTVEEVVVERNEKQAPNLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHPDHQAYPPYSPQAQPQPGHRIDSPGFKTASQQKAEASSPGPYLSPETNPAPSASAVNPHVDLTNVRLEEPSPAPPSSDSPHADSLRAPGTEAAHVQLRGPEPSLSSHVGPAKVYRKDPYPEEMMRQNHVLKQPAAVHPGQRPDKEQGLSYEPQPPYTERQAGRELEQPAYRYDASSYPEQFARNYDHRLRYDDRLLTYEEQWPYYDDKQPYQPRPLDGQPPAEPTERGYFPRFEEPVPLSYEGRAHYDPLPRTSTLRHEEPPAPAPAPGYDVHGRYRPDAQPYSTAGPKVPPEPKPYLDQYPRSYEPAPAQGFPSRAGPGHYEPLHSTTLAPPLGPSSQHKPEVPPTGSKPLPPPPAPAEEEEDPAMKPQSVLTRVKMFENKRSASMENRKDESHSTSFKPSEVTSKPAGAPVPGPKAPAPGQLGEHDRAAHRIPEPQKPHVKPPEDIVRANHYDPEEDEEYYRKQLSYFDRRSFEAKPPTHIPTSHLSEPTRPAHPQSQPQFAGYSSKGKAAEAEAVDRSFGEKRYDPLQATPPPPPLPAQYSQTAQPSPNTALGLHAHTKGAHGEGNAMSLDFQNSLVSKPDPPPSQNKPAAYRPAPREDAVQSAFYPQKSFPEKAPANGVEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPNETAHKPDLSSKAPASPKTLLKSAQPPEFDSGVETFSVHTDNKLKYQVNSVSTGPRAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006066396.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006066396.1

ORF Insert Sequence:

ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAACAATGG AGGAAACAGC TATATGGGAA 
CAACACACGG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ATCCACATTT CCAGAGCGGG GAAACCTCCA TCGTGATTTC AGATGTGCTA 
AAGGGGGGGC CTGCAGAAGG ACAGCTGCAG GAAAATGACC GGGTGGCAAT GGTTAATGGA 
GTCTCCATGG ATAATGTTGA GCATGCGTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACCATTCG AAGGAAGAAG AAAGTTCAGA TCCCAGTAAA TCGTCCTGAT 
CCTGAGCCTG TGTCTGAGAA CGAAGAGGAC AGTTACGACG AGGAGGCGCA TGACCCCAGA 
AGTGGCCGTG GGGCCCTGGC AAACAGGAGG AGCGAGAAGA GCTGGGGCCG AGATAGGAGT 
GCCAGTCGGG AGAGGAGTCT GTCCCCGAGG TCTGACAGGC GGTCTGTGGC CTCCAGTCAG 
CCTGCCAGAC CCACCAAAGT CACCCTGGTG AAGTCCCGGA AGAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTGAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC GGAAAACATG 
TCACTGACAG ATGCGAAGAC GCTGATCGAG AGGTCTAAGG GCAAGTTAAA GATGGTGGTT 
CAGAGAGATG AGCGGGCTAC CCTGCTGAAT GTCCCTGACC TTTCTGACAG CATCCACTCT 
GCTGACGCCT CCGAGAGGGA CGATATTTCA GAAATCCAGT CACTGGCATC AGACCATTCG 
GGTCGATCCC ACGACCGGCC TCCCCGTCAC AGCCGCTCTC GGTCTCCTGA CCAGCGGTCC 
GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAGTGGCAG TCTCCGGAGC 
AGAGAGGAAG AGAGAATTTC TAAACCAGGG GCTGTCTCGA CTCCGGTAAA GCATGCAGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT GTAGTTGAGC GAAATGAGAA ACAAGCACCC 
AATCTTCCAG AACCAAAGCC TGTGTATGCC CAGGTAGGAC AGCCAGACGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTGCCCAAC TCAACTCATG AAGATGGCAT TCTTCGGCCC 
AGCATGAAGT TAGTAAAATT CAGGAAAGGA GATAGTGTGG GTCTTCGGCT AGCTGGTGGA 
AACGATGTTG GCATATTTGT AGCTGGTGTT CTCGAAGACA GCCCTGCTGC CAAAGAAGGC 
TTGGAGGAGG GCGACCAGAT CCTCAGGGTA AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAAGCCG TCCTCTTCCT GCTTGACCTC CCAAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAAA AAGATGTTTA TCGTCGCATC GTAGAATCCG ATGTGGGTGA TTCTTTCTAT 
ATCAGAACCC ATTTTGAATA TGAGAAGGAA TCTCCCTATG GACTCAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTCGTGGA CACTCTGTAC AATGGGAAGC TGGGGTCCTG GCTCGCCATT 
CGGATTGGCA AAAACCATAA AGAGGTGGAA CGTGGCATCA TCCCAAACAA AAACAGAGCT 
GAGCAGTTAG CCAGTGTGCA GTATACACTT CCCAAGACAG CAGGGGGAGA CCGCGCTGAC 
TTCTGGAGGT TCCGCGGTCT GCGCAGCTCC AAGAGAAACC TGCGCAAGAG CCGAGAAGAC 
CTCTCAGCCC AGCCAGTCCA GACCAAGTTC CCGGCGTATG AGAGGGTGGT TCTTCGAGAA 
GCTGGATTCC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGGGAGAAG 
CTGGCAAGAG AGGAGCCAGA TATTTACCAG ATCGCAAAAA GTGAACCTCG GGATGCAGGA 
ACCGACCAGC GCAGCTCTGG CATTATTCGC CTCCACACAA TAAAGCAGAT AATAGATCAG 
GACAAACATG CCTTGCTGGA TGTAACGCCC AATGCAGTAG ACCGTCTGAA CTATGCCCAG 
TGGTATCCAA TAGTGGTGTT CCTCAACCCG GACTCAAAGC AGGGTGTGAA AACAATGCGG 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGC GCCAGGAAGT TATATGAGCG CTCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGCTGGTATG GTGCATTGAA AGAAGCAATC CAGCAACAAC AGAACCAGTT GGTATGGGTT 
TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAG 
ACCCTAAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACCCGGTC CTCTGAGCCT 
GTCAGAGAGG ACTCCTCTGG AATGCATCCT GACCACCAGG CATATCCTCC CTACTCACCA 
CAAGCGCAGC CACAGCCAGG CCATAGAATA GACTCCCCTG GATTTAAAAC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGGCCCT TACCTTTCGC CTGAAACAAA CCCGGCACCA 
TCAGCCTCTG CTGTTAATCC TCACGTAGAC TTAACTAATG TCAGACTGGA GGAGCCGAGC 
CCAGCTCCCC CTTCCTCTGA CTCACCACAC GCCGACTCTT TACGAGCTCC AGGCACTGAG 
GCCGCTCACG TGCAGCTCAG AGGTCCAGAG CCGTCCCTGT CGTCGCATGT GGGCCCAGCA 
AAGGTGTACA GGAAGGACCC CTACCCTGAG GAGATGATGA GACAGAACCA CGTCTTGAAG 
CAGCCGGCTG CGGTTCACCC TGGGCAGAGG CCAGACAAGG AGCAAGGCCT GAGCTACGAG 
CCGCAGCCTC CGTACACAGA GCGACAGGCT GGCAGGGAGT TGGAGCAGCC TGCATACAGA 
TATGATGCCT CTAGCTACCC GGAGCAGTTT GCTCGGAACT ATGACCATCG TCTGCGCTAT 
GATGACCGCC TCCTCACCTA CGAGGAGCAG TGGCCCTACT ACGACGACAA GCAGCCTTAC 
CAGCCCCGGC CTCTTGATGG TCAGCCTCCC GCGGAGCCAA CAGAACGAGG GTATTTTCCC 
CGTTTTGAGG AGCCAGTGCC CCTGTCCTAT GAAGGCCGAG CGCACTATGA CCCACTGCCC 
AGGACCTCCA CCTTGCGGCA CGAAGAGCCT CCTGCCCCTG CCCCTGCCCC TGGGTACGAC 
GTGCACGGCA GGTACCGGCC TGACGCGCAG CCCTACTCCA CTGCTGGCCC CAAGGTGCCC 
CCTGAGCCCA AGCCGTACTT GGACCAGTAC CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG 
GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC TACGAGCCTC TTCACAGCAC CACGCTCGCT 
CCACCACTTG GCCCCTCGTC CCAGCACAAG CCCGAAGTCC CGCCCACAGG CTCCAAACCC 
CTGCCTCCAC CCCCAGCTCC AGCTGAGGAG GAGGAAGACC CAGCCATGAA GCCACAATCC 
GTGCTCACCA GAGTGAAGAT GTTTGAGAAC AAGAGGTCTG CTTCCATGGA GAACAGGAAG 
GATGAGAGCC ATTCCACTAG TTTCAAGCCC TCAGAAGTCA CGTCAAAACC GGCAGGTGCT 
CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG GGTCAGCTCG GTGAACACGA CAGAGCAGCG 
CATAGGATTC CTGAACCACA GAAGCCTCAC GTGAAGCCAC CTGAAGACAT TGTTCGGGCA 
AATCACTATG ACCCCGAGGA GGATGAGGAG TACTACCGGA AGCAGCTCTC CTACTTCGAT 
CGGAGAAGCT TTGAGGCCAA GCCCCCCACC CACATCCCCA CTAGCCACCT CTCCGAGCCC 
ACCAGGCCGG CTCACCCCCA ATCTCAGCCA CAGTTTGCCG GTTACTCTTC CAAGGGGAAG 
GCTGCTGAGG CCGAAGCTGT GGACAGGTCA TTCGGGGAGA AGCGCTATGA CCCGCTGCAG 
GCGACGCCCC CCCCTCCGCC GCTGCCCGCT CAGTACAGCC AGACTGCTCA ACCCAGCCCC 
AACACGGCTC TCGGCCTCCA CGCACACACC AAGGGGGCGC ACGGTGAAGG TAATGCCATG 
TCATTGGATT TTCAGAACTC GTTAGTGTCC AAACCAGACC CACCTCCATC TCAGAATAAG 
CCAGCAGCTT ACAGACCAGC CCCTCGAGAG GATGCTGTCC AGTCTGCCTT CTACCCACAA 
AAGAGTTTCC CAGAGAAAGC CCCAGCTAAT GGGGTCGAGC AGACGCAGAA AACCGTCACG 
CCAGCATACA ATCGATTCAC ACCAAAACCA TACACAAGCT CTGCACGGCC GTTTGAACGC 
AAGTTTGAAA GCCCTAAATT CAACCACAAT CTCTTGCCGA ATGAAACCGC ACACAAACCT 
GACCTGTCCT CCAAAGCCCC TGCTTCTCCG AAAACCCTCC TGAAGTCTGC ACAGCCTCCT 
GAGTTTGACA GTGGTGTGGA GACCTTCTCT GTCCACACAG ACAACAAGCT GAAATACCAA 
GTAAACAGTG TTAGCACGGG GCCGAGAGCA GTGCCCGTGA GTCCCTCAGC CGTGGAGGAA 
GATGAAGATG AAGACGGTCA CACAGTGGTG GCTACTGCCC GCGGCGTGTT TAACAGCAAT 
GGTGGGGTGC TGAGCTCCAT TGAGACCGGT GTCAGCATCA TCATCCCCCA GGGAGCCATT 
CCTGAGGGGG TGGAACAGGA GATCTACTTC AAGGTCTGCC GCGACAACAG CATCCTGCCG 
CCTTTGGACA AAGAGAAGGG TGAGACCCTG CTGAGCCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCCTACCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCAAAT TATCTTGTTG GAGCAAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_006066396.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006066458.1 Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X5, mRNA.

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CloneID	OBu21758
Clone ID Related Accession (Same CDS sequence)	XM_006066396.1 , XM_006066396.2
Accession Version	XM_006066396.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	tight junction protein ZO-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006066396.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAACAATGG AGGAAACAGC TATATGGGAA 
CAACACACGG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ATCCACATTT CCAGAGCGGG GAAACCTCCA TCGTGATTTC AGATGTGCTA 
AAGGGGGGGC CTGCAGAAGG ACAGCTGCAG GAAAATGACC GGGTGGCAAT GGTTAATGGA 
GTCTCCATGG ATAATGTTGA GCATGCGTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACCATTCG AAGGAAGAAG AAAGTTCAGA TCCCAGTAAA TCGTCCTGAT 
CCTGAGCCTG TGTCTGAGAA CGAAGAGGAC AGTTACGACG AGGAGGCGCA TGACCCCAGA 
AGTGGCCGTG GGGCCCTGGC AAACAGGAGG AGCGAGAAGA GCTGGGGCCG AGATAGGAGT 
GCCAGTCGGG AGAGGAGTCT GTCCCCGAGG TCTGACAGGC GGTCTGTGGC CTCCAGTCAG 
CCTGCCAGAC CCACCAAAGT CACCCTGGTG AAGTCCCGGA AGAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTGAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC GGAAAACATG 
TCACTGACAG ATGCGAAGAC GCTGATCGAG AGGTCTAAGG GCAAGTTAAA GATGGTGGTT 
CAGAGAGATG AGCGGGCTAC CCTGCTGAAT GTCCCTGACC TTTCTGACAG CATCCACTCT 
GCTGACGCCT CCGAGAGGGA CGATATTTCA GAAATCCAGT CACTGGCATC AGACCATTCG 
GGTCGATCCC ACGACCGGCC TCCCCGTCAC AGCCGCTCTC GGTCTCCTGA CCAGCGGTCC 
GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAGTGGCAG TCTCCGGAGC 
AGAGAGGAAG AGAGAATTTC TAAACCAGGG GCTGTCTCGA CTCCGGTAAA GCATGCAGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT GTAGTTGAGC GAAATGAGAA ACAAGCACCC 
AATCTTCCAG AACCAAAGCC TGTGTATGCC CAGGTAGGAC AGCCAGACGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTGCCCAAC TCAACTCATG AAGATGGCAT TCTTCGGCCC 
AGCATGAAGT TAGTAAAATT CAGGAAAGGA GATAGTGTGG GTCTTCGGCT AGCTGGTGGA 
AACGATGTTG GCATATTTGT AGCTGGTGTT CTCGAAGACA GCCCTGCTGC CAAAGAAGGC 
TTGGAGGAGG GCGACCAGAT CCTCAGGGTA AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAAGCCG TCCTCTTCCT GCTTGACCTC CCAAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAAA AAGATGTTTA TCGTCGCATC GTAGAATCCG ATGTGGGTGA TTCTTTCTAT 
ATCAGAACCC ATTTTGAATA TGAGAAGGAA TCTCCCTATG GACTCAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTCGTGGA CACTCTGTAC AATGGGAAGC TGGGGTCCTG GCTCGCCATT 
CGGATTGGCA AAAACCATAA AGAGGTGGAA CGTGGCATCA TCCCAAACAA AAACAGAGCT 
GAGCAGTTAG CCAGTGTGCA GTATACACTT CCCAAGACAG CAGGGGGAGA CCGCGCTGAC 
TTCTGGAGGT TCCGCGGTCT GCGCAGCTCC AAGAGAAACC TGCGCAAGAG CCGAGAAGAC 
CTCTCAGCCC AGCCAGTCCA GACCAAGTTC CCGGCGTATG AGAGGGTGGT TCTTCGAGAA 
GCTGGATTCC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGGGAGAAG 
CTGGCAAGAG AGGAGCCAGA TATTTACCAG ATCGCAAAAA GTGAACCTCG GGATGCAGGA 
ACCGACCAGC GCAGCTCTGG CATTATTCGC CTCCACACAA TAAAGCAGAT AATAGATCAG 
GACAAACATG CCTTGCTGGA TGTAACGCCC AATGCAGTAG ACCGTCTGAA CTATGCCCAG 
TGGTATCCAA TAGTGGTGTT CCTCAACCCG GACTCAAAGC AGGGTGTGAA AACAATGCGG 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGC GCCAGGAAGT TATATGAGCG CTCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGCTGGTATG GTGCATTGAA AGAAGCAATC CAGCAACAAC AGAACCAGTT GGTATGGGTT 
TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAG 
ACCCTAAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACCCGGTC CTCTGAGCCT 
GTCAGAGAGG ACTCCTCTGG AATGCATCCT GACCACCAGG CATATCCTCC CTACTCACCA 
CAAGCGCAGC CACAGCCAGG CCATAGAATA GACTCCCCTG GATTTAAAAC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGGCCCT TACCTTTCGC CTGAAACAAA CCCGGCACCA 
TCAGCCTCTG CTGTTAATCC TCACGTAGAC TTAACTAATG TCAGACTGGA GGAGCCGAGC 
CCAGCTCCCC CTTCCTCTGA CTCACCACAC GCCGACTCTT TACGAGCTCC AGGCACTGAG 
GCCGCTCACG TGCAGCTCAG AGGTCCAGAG CCGTCCCTGT CGTCGCATGT GGGCCCAGCA 
AAGGTGTACA GGAAGGACCC CTACCCTGAG GAGATGATGA GACAGAACCA CGTCTTGAAG 
CAGCCGGCTG CGGTTCACCC TGGGCAGAGG CCAGACAAGG AGCAAGGCCT GAGCTACGAG 
CCGCAGCCTC CGTACACAGA GCGACAGGCT GGCAGGGAGT TGGAGCAGCC TGCATACAGA 
TATGATGCCT CTAGCTACCC GGAGCAGTTT GCTCGGAACT ATGACCATCG TCTGCGCTAT 
GATGACCGCC TCCTCACCTA CGAGGAGCAG TGGCCCTACT ACGACGACAA GCAGCCTTAC 
CAGCCCCGGC CTCTTGATGG TCAGCCTCCC GCGGAGCCAA CAGAACGAGG GTATTTTCCC 
CGTTTTGAGG AGCCAGTGCC CCTGTCCTAT GAAGGCCGAG CGCACTATGA CCCACTGCCC 
AGGACCTCCA CCTTGCGGCA CGAAGAGCCT CCTGCCCCTG CCCCTGCCCC TGGGTACGAC 
GTGCACGGCA GGTACCGGCC TGACGCGCAG CCCTACTCCA CTGCTGGCCC CAAGGTGCCC 
CCTGAGCCCA AGCCGTACTT GGACCAGTAC CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG 
GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC TACGAGCCTC TTCACAGCAC CACGCTCGCT 
CCACCACTTG GCCCCTCGTC CCAGCACAAG CCCGAAGTCC CGCCCACAGG CTCCAAACCC 
CTGCCTCCAC CCCCAGCTCC AGCTGAGGAG GAGGAAGACC CAGCCATGAA GCCACAATCC 
GTGCTCACCA GAGTGAAGAT GTTTGAGAAC AAGAGGTCTG CTTCCATGGA GAACAGGAAG 
GATGAGAGCC ATTCCACTAG TTTCAAGCCC TCAGAAGTCA CGTCAAAACC GGCAGGTGCT 
CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG GGTCAGCTCG GTGAACACGA CAGAGCAGCG 
CATAGGATTC CTGAACCACA GAAGCCTCAC GTGAAGCCAC CTGAAGACAT TGTTCGGGCA 
AATCACTATG ACCCCGAGGA GGATGAGGAG TACTACCGGA AGCAGCTCTC CTACTTCGAT 
CGGAGAAGCT TTGAGGCCAA GCCCCCCACC CACATCCCCA CTAGCCACCT CTCCGAGCCC 
ACCAGGCCGG CTCACCCCCA ATCTCAGCCA CAGTTTGCCG GTTACTCTTC CAAGGGGAAG 
GCTGCTGAGG CCGAAGCTGT GGACAGGTCA TTCGGGGAGA AGCGCTATGA CCCGCTGCAG 
GCGACGCCCC CCCCTCCGCC GCTGCCCGCT CAGTACAGCC AGACTGCTCA ACCCAGCCCC 
AACACGGCTC TCGGCCTCCA CGCACACACC AAGGGGGCGC ACGGTGAAGG TAATGCCATG 
TCATTGGATT TTCAGAACTC GTTAGTGTCC AAACCAGACC CACCTCCATC TCAGAATAAG 
CCAGCAGCTT ACAGACCAGC CCCTCGAGAG GATGCTGTCC AGTCTGCCTT CTACCCACAA 
AAGAGTTTCC CAGAGAAAGC CCCAGCTAAT GGGGTCGAGC AGACGCAGAA AACCGTCACG 
CCAGCATACA ATCGATTCAC ACCAAAACCA TACACAAGCT CTGCACGGCC GTTTGAACGC 
AAGTTTGAAA GCCCTAAATT CAACCACAAT CTCTTGCCGA ATGAAACCGC ACACAAACCT 
GACCTGTCCT CCAAAGCCCC TGCTTCTCCG AAAACCCTCC TGAAGTCTGC ACAGCCTCCT 
GAGTTTGACA GTGGTGTGGA GACCTTCTCT GTCCACACAG ACAACAAGCT GAAATACCAA 
GTAAACAGTG TTAGCACGGG GCCGAGAGCA GTGCCCGTGA GTCCCTCAGC CGTGGAGGAA 
GATGAAGATG AAGACGGTCA CACAGTGGTG GCTACTGCCC GCGGCGTGTT TAACAGCAAT 
GGTGGGGTGC TGAGCTCCAT TGAGACCGGT GTCAGCATCA TCATCCCCCA GGGAGCCATT 
CCTGAGGGGG TGGAACAGGA GATCTACTTC AAGGTCTGCC GCGACAACAG CATCCTGCCG 
CCTTTGGACA AAGAGAAGGG TGAGACCCTG CTGAGCCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCCTACCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCAAAT TATCTTGTTG GAGCAAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006066458.1
CDS	141..5438
Translation	MSARAAAAKNTTMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVNRPDPEPVSENEEDSYDEEAHDPRSGRGALANRRSEKSWGRDRSASRERSLSPRSDRRSVASSQPARPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSADASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTVEEVVVERNEKQAPNLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHPDHQAYPPYSPQAQPQPGHRIDSPGFKTASQQKAEASSPGPYLSPETNPAPSASAVNPHVDLTNVRLEEPSPAPPSSDSPHADSLRAPGTEAAHVQLRGPEPSLSSHVGPAKVYRKDPYPEEMMRQNHVLKQPAAVHPGQRPDKEQGLSYEPQPPYTERQAGRELEQPAYRYDASSYPEQFARNYDHRLRYDDRLLTYEEQWPYYDDKQPYQPRPLDGQPPAEPTERGYFPRFEEPVPLSYEGRAHYDPLPRTSTLRHEEPPAPAPAPGYDVHGRYRPDAQPYSTAGPKVPPEPKPYLDQYPRSYEPAPAQGFPSRAGPGHYEPLHSTTLAPPLGPSSQHKPEVPPTGSKPLPPPPAPAEEEEDPAMKPQSVLTRVKMFENKRSASMENRKDESHSTSFKPSEVTSKPAGAPVPGPKAPAPGQLGEHDRAAHRIPEPQKPHVKPPEDIVRANHYDPEEDEEYYRKQLSYFDRRSFEAKPPTHIPTSHLSEPTRPAHPQSQPQFAGYSSKGKAAEAEAVDRSFGEKRYDPLQATPPPPPLPAQYSQTAQPSPNTALGLHAHTKGAHGEGNAMSLDFQNSLVSKPDPPPSQNKPAAYRPAPREDAVQSAFYPQKSFPEKAPANGVEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPNETAHKPDLSSKAPASPKTLLKSAQPPEFDSGVETFSVHTDNKLKYQVNSVSTGPRAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006066396.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006066396.2

ORF Insert Sequence:

ATGTCCGCCA GGGCCGCGGC CGCCAAGAAC ACAACAATGG AGGAAACAGC TATATGGGAA 
CAACACACGG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGAAGAGATA ATCCACATTT CCAGAGCGGG GAAACCTCCA TCGTGATTTC AGATGTGCTA 
AAGGGGGGGC CTGCAGAAGG ACAGCTGCAG GAAAATGACC GGGTGGCAAT GGTTAATGGA 
GTCTCCATGG ATAATGTTGA GCATGCGTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACCATTCG AAGGAAGAAG AAAGTTCAGA TCCCAGTAAA TCGTCCTGAT 
CCTGAGCCTG TGTCTGAGAA CGAAGAGGAC AGTTACGACG AGGAGGCGCA TGACCCCAGA 
AGTGGCCGTG GGGCCCTGGC AAACAGGAGG AGCGAGAAGA GCTGGGGCCG AGATAGGAGT 
GCCAGTCGGG AGAGGAGTCT GTCCCCGAGG TCTGACAGGC GGTCTGTGGC CTCCAGTCAG 
CCTGCCAGAC CCACCAAAGT CACCCTGGTG AAGTCCCGGA AGAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTGAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACCGTGAC GGAAAACATG 
TCACTGACAG ATGCGAAGAC GCTGATCGAG AGGTCTAAGG GCAAGTTAAA GATGGTGGTT 
CAGAGAGATG AGCGGGCTAC CCTGCTGAAT GTCCCTGACC TTTCTGACAG CATCCACTCT 
GCTGACGCCT CCGAGAGGGA CGATATTTCA GAAATCCAGT CACTGGCATC AGACCATTCG 
GGTCGATCCC ACGACCGGCC TCCCCGTCAC AGCCGCTCTC GGTCTCCTGA CCAGCGGTCC 
GAGCCTTCTG ACCATTCTAG ACACTCTCCA CAGCAGCCCA GCAGTGGCAG TCTCCGGAGC 
AGAGAGGAAG AGAGAATTTC TAAACCAGGG GCTGTCTCGA CTCCGGTAAA GCATGCAGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT GTAGTTGAGC GAAATGAGAA ACAAGCACCC 
AATCTTCCAG AACCAAAGCC TGTGTATGCC CAGGTAGGAC AGCCAGACGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTGCCCAAC TCAACTCATG AAGATGGCAT TCTTCGGCCC 
AGCATGAAGT TAGTAAAATT CAGGAAAGGA GATAGTGTGG GTCTTCGGCT AGCTGGTGGA 
AACGATGTTG GCATATTTGT AGCTGGTGTT CTCGAAGACA GCCCTGCTGC CAAAGAAGGC 
TTGGAGGAGG GCGACCAGAT CCTCAGGGTA AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAAGCCG TCCTCTTCCT GCTTGACCTC CCAAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAAA AAGATGTTTA TCGTCGCATC GTAGAATCCG ATGTGGGTGA TTCTTTCTAT 
ATCAGAACCC ATTTTGAATA TGAGAAGGAA TCTCCCTATG GACTCAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTCGTGGA CACTCTGTAC AATGGGAAGC TGGGGTCCTG GCTCGCCATT 
CGGATTGGCA AAAACCATAA AGAGGTGGAA CGTGGCATCA TCCCAAACAA AAACAGAGCT 
GAGCAGTTAG CCAGTGTGCA GTATACACTT CCCAAGACAG CAGGGGGAGA CCGCGCTGAC 
TTCTGGAGGT TCCGCGGTCT GCGCAGCTCC AAGAGAAACC TGCGCAAGAG CCGAGAAGAC 
CTCTCAGCCC AGCCAGTCCA GACCAAGTTC CCGGCGTATG AGAGGGTGGT TCTTCGAGAA 
GCTGGATTCC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGGGAGAAG 
CTGGCAAGAG AGGAGCCAGA TATTTACCAG ATCGCAAAAA GTGAACCTCG GGATGCAGGA 
ACCGACCAGC GCAGCTCTGG CATTATTCGC CTCCACACAA TAAAGCAGAT AATAGATCAG 
GACAAACATG CCTTGCTGGA TGTAACGCCC AATGCAGTAG ACCGTCTGAA CTATGCCCAG 
TGGTATCCAA TAGTGGTGTT CCTCAACCCG GACTCAAAGC AGGGTGTGAA AACAATGCGG 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGC GCCAGGAAGT TATATGAGCG CTCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGCTGGTATG GTGCATTGAA AGAAGCAATC CAGCAACAAC AGAACCAGTT GGTATGGGTT 
TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAG 
ACCCTAAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACCCGGTC CTCTGAGCCT 
GTCAGAGAGG ACTCCTCTGG AATGCATCCT GACCACCAGG CATATCCTCC CTACTCACCA 
CAAGCGCAGC CACAGCCAGG CCATAGAATA GACTCCCCTG GATTTAAAAC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGGCCCT TACCTTTCGC CTGAAACAAA CCCGGCACCA 
TCAGCCTCTG CTGTTAATCC TCACGTAGAC TTAACTAATG TCAGACTGGA GGAGCCGAGC 
CCAGCTCCCC CTTCCTCTGA CTCACCACAC GCCGACTCTT TACGAGCTCC AGGCACTGAG 
GCCGCTCACG TGCAGCTCAG AGGTCCAGAG CCGTCCCTGT CGTCGCATGT GGGCCCAGCA 
AAGGTGTACA GGAAGGACCC CTACCCTGAG GAGATGATGA GACAGAACCA CGTCTTGAAG 
CAGCCGGCTG CGGTTCACCC TGGGCAGAGG CCAGACAAGG AGCAAGGCCT GAGCTACGAG 
CCGCAGCCTC CGTACACAGA GCGACAGGCT GGCAGGGAGT TGGAGCAGCC TGCATACAGA 
TATGATGCCT CTAGCTACCC GGAGCAGTTT GCTCGGAACT ATGACCATCG TCTGCGCTAT 
GATGACCGCC TCCTCACCTA CGAGGAGCAG TGGCCCTACT ACGACGACAA GCAGCCTTAC 
CAGCCCCGGC CTCTTGATGG TCAGCCTCCC GCGGAGCCAA CAGAACGAGG GTATTTTCCC 
CGTTTTGAGG AGCCAGTGCC CCTGTCCTAT GAAGGCCGAG CGCACTATGA CCCACTGCCC 
AGGACCTCCA CCTTGCGGCA CGAAGAGCCT CCTGCCCCTG CCCCTGCCCC TGGGTACGAC 
GTGCACGGCA GGTACCGGCC TGACGCGCAG CCCTACTCCA CTGCTGGCCC CAAGGTGCCC 
CCTGAGCCCA AGCCGTACTT GGACCAGTAC CCTCGGAGTT ACGAGCCAGC GCCAGCCCAG 
GGCTTCCCCT CCAGGGCAGG CCCAGGCCAC TACGAGCCTC TTCACAGCAC CACGCTCGCT 
CCACCACTTG GCCCCTCGTC CCAGCACAAG CCCGAAGTCC CGCCCACAGG CTCCAAACCC 
CTGCCTCCAC CCCCAGCTCC AGCTGAGGAG GAGGAAGACC CAGCCATGAA GCCACAATCC 
GTGCTCACCA GAGTGAAGAT GTTTGAGAAC AAGAGGTCTG CTTCCATGGA GAACAGGAAG 
GATGAGAGCC ATTCCACTAG TTTCAAGCCC TCAGAAGTCA CGTCAAAACC GGCAGGTGCT 
CCTGTCCCAG GTCCCAAAGC CCCTGCTCCG GGTCAGCTCG GTGAACACGA CAGAGCAGCG 
CATAGGATTC CTGAACCACA GAAGCCTCAC GTGAAGCCAC CTGAAGACAT TGTTCGGGCA 
AATCACTATG ACCCCGAGGA GGATGAGGAG TACTACCGGA AGCAGCTCTC CTACTTCGAT 
CGGAGAAGCT TTGAGGCCAA GCCCCCCACC CACATCCCCA CTAGCCACCT CTCCGAGCCC 
ACCAGGCCGG CTCACCCCCA ATCTCAGCCA CAGTTTGCCG GTTACTCTTC CAAGGGGAAG 
GCTGCTGAGG CCGAAGCTGT GGACAGGTCA TTCGGGGAGA AGCGCTATGA CCCGCTGCAG 
GCGACGCCCC CCCCTCCGCC GCTGCCCGCT CAGTACAGCC AGACTGCTCA ACCCAGCCCC 
AACACGGCTC TCGGCCTCCA CGCACACACC AAGGGGGCGC ACGGTGAAGG TAATGCCATG 
TCATTGGATT TTCAGAACTC GTTAGTGTCC AAACCAGACC CACCTCCATC TCAGAATAAG 
CCAGCAGCTT ACAGACCAGC CCCTCGAGAG GATGCTGTCC AGTCTGCCTT CTACCCACAA 
AAGAGTTTCC CAGAGAAAGC CCCAGCTAAT GGGGTCGAGC AGACGCAGAA AACCGTCACG 
CCAGCATACA ATCGATTCAC ACCAAAACCA TACACAAGCT CTGCACGGCC GTTTGAACGC 
AAGTTTGAAA GCCCTAAATT CAACCACAAT CTCTTGCCGA ATGAAACCGC ACACAAACCT 
GACCTGTCCT CCAAAGCCCC TGCTTCTCCG AAAACCCTCC TGAAGTCTGC ACAGCCTCCT 
GAGTTTGACA GTGGTGTGGA GACCTTCTCT GTCCACACAG ACAACAAGCT GAAATACCAA 
GTAAACAGTG TTAGCACGGG GCCGAGAGCA GTGCCCGTGA GTCCCTCAGC CGTGGAGGAA 
GATGAAGATG AAGACGGTCA CACAGTGGTG GCTACTGCCC GCGGCGTGTT TAACAGCAAT 
GGTGGGGTGC TGAGCTCCAT TGAGACCGGT GTCAGCATCA TCATCCCCCA GGGAGCCATT 
CCTGAGGGGG TGGAACAGGA GATCTACTTC AAGGTCTGCC GCGACAACAG CATCCTGCCG 
CCTTTGGACA AAGAGAAGGG TGAGACCCTG CTGAGCCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCCTACCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCAAAT TATCTTGTTG GAGCAAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_006066397.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006066459.1 Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X5, mRNA.

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CloneID	OBu21759
Clone ID Related Accession (Same CDS sequence)	XM_006066397.1 , XM_006066397.2
Accession Version	XM_006066397.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	tight junction protein ZO-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785206.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTCGC CACTGGACAA GGAGAACACA ACAATGGAGG AAACAGCTAT ATGGGAACAA 
CACACGGTGA CACTTCACAG GGCTCCTGGA TTTGGATTTG GAATTGCAAT ATCTGGTGGA 
AGAGATAATC CACATTTCCA GAGCGGGGAA ACCTCCATCG TGATTTCAGA TGTGCTAAAG 
GGGGGGCCTG CAGAAGGACA GCTGCAGGAA AATGACCGGG TGGCAATGGT TAATGGAGTC 
TCCATGGATA ATGTTGAGCA TGCGTTTGCT GTTCAGCAAC TAAGGAAAAG TGGGAAAAAT 
GCAAAAATTA CCATTCGAAG GAAGAAGAAA GTTCAGATCC CAGTAAATCG TCCTGATCCT 
GAGCCTGTGT CTGAGAACGA AGAGGACAGT TACGACGAGG AGGCGCATGA CCCCAGAAGT 
GGCCGTGGGG CCCTGGCAAA CAGGAGGAGC GAGAAGAGCT GGGGCCGAGA TAGGAGTGCC 
AGTCGGGAGA GGAGTCTGTC CCCGAGGTCT GACAGGCGGT CTGTGGCCTC CAGTCAGCCT 
GCCAGACCCA CCAAAGTCAC CCTGGTGAAG TCCCGGAAGA ATGAAGAATA TGGTCTTCGA 
TTGGCCAGCC ATATATTTGT GAAGGAAATT TCACAAGATA GTTTGGCAGC AAGAGATGGC 
AATATTCAAG AAGGCGATGT TGTATTGAAG ATAAATGGTA CCGTGACGGA AAACATGTCA 
CTGACAGATG CGAAGACGCT GATCGAGAGG TCTAAGGGCA AGTTAAAGAT GGTGGTTCAG 
AGAGATGAGC GGGCTACCCT GCTGAATGTC CCTGACCTTT CTGACAGCAT CCACTCTGCT 
GACGCCTCCG AGAGGGACGA TATTTCAGAA ATCCAGTCAC TGGCATCAGA CCATTCGGGT 
CGATCCCACG ACCGGCCTCC CCGTCACAGC CGCTCTCGGT CTCCTGACCA GCGGTCCGAG 
CCTTCTGACC ATTCTAGACA CTCTCCACAG CAGCCCAGCA GTGGCAGTCT CCGGAGCAGA 
GAGGAAGAGA GAATTTCTAA ACCAGGGGCT GTCTCGACTC CGGTAAAGCA TGCAGATGAT 
CACACACCTA AAACAGTGGA AGAAGTTGTA GTTGAGCGAA ATGAGAAACA AGCACCCAAT 
CTTCCAGAAC CAAAGCCTGT GTATGCCCAG GTAGGACAGC CAGACGTGGA TTTACCTGTC 
AGTCCATCTG ATGGTGTCCT GCCCAACTCA ACTCATGAAG ATGGCATTCT TCGGCCCAGC 
ATGAAGTTAG TAAAATTCAG GAAAGGAGAT AGTGTGGGTC TTCGGCTAGC TGGTGGAAAC 
GATGTTGGCA TATTTGTAGC TGGTGTTCTC GAAGACAGCC CTGCTGCCAA AGAAGGCTTG 
GAGGAGGGCG ACCAGATCCT CAGGGTAAAC AATGTAGATT TCACAAATAT CATAAGAGAA 
GAAGCCGTCC TCTTCCTGCT TGACCTCCCA AAAGGAGAAG AAGTGACCAT ATTGGCTCAG 
AAGAAAAAAG ATGTTTATCG TCGCATCGTA GAATCCGATG TGGGTGATTC TTTCTATATC 
AGAACCCATT TTGAATATGA GAAGGAATCT CCCTATGGAC TCAGTTTTAA CAAAGGAGAG 
GTGTTCCGTG TCGTGGACAC TCTGTACAAT GGGAAGCTGG GGTCCTGGCT CGCCATTCGG 
ATTGGCAAAA ACCATAAAGA GGTGGAACGT GGCATCATCC CAAACAAAAA CAGAGCTGAG 
CAGTTAGCCA GTGTGCAGTA TACACTTCCC AAGACAGCAG GGGGAGACCG CGCTGACTTC 
TGGAGGTTCC GCGGTCTGCG CAGCTCCAAG AGAAACCTGC GCAAGAGCCG AGAAGACCTC 
TCAGCCCAGC CAGTCCAGAC CAAGTTCCCG GCGTATGAGA GGGTGGTTCT TCGAGAAGCT 
GGATTCCTGA GGCCTGTAAC CATCTTTGGA CCAATAGCTG ATGTTGCCAG GGAGAAGCTG 
GCAAGAGAGG AGCCAGATAT TTACCAGATC GCAAAAAGTG AACCTCGGGA TGCAGGAACC 
GACCAGCGCA GCTCTGGCAT TATTCGCCTC CACACAATAA AGCAGATAAT AGATCAGGAC 
AAACATGCCT TGCTGGATGT AACGCCCAAT GCAGTAGACC GTCTGAACTA TGCCCAGTGG 
TATCCAATAG TGGTGTTCCT CAACCCGGAC TCAAAGCAGG GTGTGAAAAC AATGCGGATG 
AGGTTATGTC CAGAATCTCG GAAAAGCGCC AGGAAGTTAT ATGAGCGCTC TCATAAACTT 
CGTAAAAATA ATCACCATCT TTTTACAACT ACAATTAACT TAAATTCAAT GAATGATGGC 
TGGTATGGTG CATTGAAAGA AGCAATCCAG CAACAACAGA ACCAGTTGGT ATGGGTTTCT 
GAGGGAAAGG CGGATGGTGC TACAAGTGAT GACCTTGATT TGCATGATGA TCGTCTGTCC 
TACCTGTCAG CCCCAGGTAG TGAATACTCA ATGTATAGCA CGGACAGTAG ACACACTTCT 
GACTATGAAG ACACAGATAC AGAAGGCGGG GCCTACACTG ATCAAGAACT AGATGAGACC 
CTAAATGATG AGGTGGGGAC TCCCCCGGAG TCTGCCATTA CCCGGTCCTC TGAGCCTGTC 
AGAGAGGACT CCTCTGGAAT GCATCCTGAC CACCAGGCAT ATCCTCCCTA CTCACCACAA 
GCGCAGCCAC AGCCAGGCCA TAGAATAGAC TCCCCTGGAT TTAAAACAGC CTCTCAACAG 
AAAGCAGAAG CCTCATCTCC AGGCCCTTAC CTTTCGCCTG AAACAAACCC GGCACCATCA 
GCCTCTGCTG TTAATCCTCA CGTAGACTTA ACTAATGTCA GACTGGAGGA GCCGAGCCCA 
GCTCCCCCTT CCTCTGACTC ACCACACGCC GACTCTTTAC GAGCTCCAGG CACTGAGGCC 
GCTCACGTGC AGCTCAGAGG TCCAGAGCCG TCCCTGTCGT CGCATGTGGG CCCAGCAAAG 
GTGTACAGGA AGGACCCCTA CCCTGAGGAG ATGATGAGAC AGAACCACGT CTTGAAGCAG 
CCGGCTGCGG TTCACCCTGG GCAGAGGCCA GACAAGGAGC AAGGCCTGAG CTACGAGCCG 
CAGCCTCCGT ACACAGAGCG ACAGGCTGGC AGGGAGTTGG AGCAGCCTGC ATACAGATAT 
GATGCCTCTA GCTACCCGGA GCAGTTTGCT CGGAACTATG ACCATCGTCT GCGCTATGAT 
GACCGCCTCC TCACCTACGA GGAGCAGTGG CCCTACTACG ACGACAAGCA GCCTTACCAG 
CCCCGGCCTC TTGATGGTCA GCCTCCCGCG GAGCCAACAG AACGAGGGTA TTTTCCCCGT 
TTTGAGGAGC CAGTGCCCCT GTCCTATGAA GGCCGAGCGC ACTATGACCC ACTGCCCAGG 
ACCTCCACCT TGCGGCACGA AGAGCCTCCT GCCCCTGCCC CTGCCCCTGG GTACGACGTG 
CACGGCAGGT ACCGGCCTGA CGCGCAGCCC TACTCCACTG CTGGCCCCAA GGTGCCCCCT 
GAGCCCAAGC CGTACTTGGA CCAGTACCCT CGGAGTTACG AGCCAGCGCC AGCCCAGGGC 
TTCCCCTCCA GGGCAGGCCC AGGCCACTAC GAGCCTCTTC ACAGCACCAC GCTCGCTCCA 
CCACTTGGCC CCTCGTCCCA GCACAAGCCC GAAGTCCCGC CCACAGGCTC CAAACCCCTG 
CCTCCACCCC CAGCTCCAGC TGAGGAGGAG GAAGACCCAG CCATGAAGCC ACAATCCGTG 
CTCACCAGAG TGAAGATGTT TGAGAACAAG AGGTCTGCTT CCATGGAGAA CAGGAAGGAT 
GAGAGCCATT CCACTAGTTT CAAGCCCTCA GAAGTCACGT CAAAACCGGC AGGTGCTCCT 
GTCCCAGGTC CCAAAGCCCC TGCTCCGGGT CAGCTCGGTG AACACGACAG AGCAGCGCAT 
AGGATTCCTG AACCACAGAA GCCTCACGTG AAGCCACCTG AAGACATTGT TCGGGCAAAT 
CACTATGACC CCGAGGAGGA TGAGGAGTAC TACCGGAAGC AGCTCTCCTA CTTCGATCGG 
AGAAGCTTTG AGGCCAAGCC CCCCACCCAC ATCCCCACTA GCCACCTCTC CGAGCCCACC 
AGGCCGGCTC ACCCCCAATC TCAGCCACAG TTTGCCGGTT ACTCTTCCAA GGGGAAGGCT 
GCTGAGGCCG AAGCTGTGGA CAGGTCATTC GGGGAGAAGC GCTATGACCC GCTGCAGGCG 
ACGCCCCCCC CTCCGCCGCT GCCCGCTCAG TACAGCCAGA CTGCTCAACC CAGCCCCAAC 
ACGGCTCTCG GCCTCCACGC ACACACCAAG GGGGCGCACG GTGAAGGTAA TGCCATGTCA 
TTGGATTTTC AGAACTCGTT AGTGTCCAAA CCAGACCCAC CTCCATCTCA GAATAAGCCA 
GCAGCTTACA GACCAGCCCC TCGAGAGGAT GCTGTCCAGT CTGCCTTCTA CCCACAAAAG 
AGTTTCCCAG AGAAAGCCCC AGCTAATGGG GTCGAGCAGA CGCAGAAAAC CGTCACGCCA 
GCATACAATC GATTCACACC AAAACCATAC ACAAGCTCTG CACGGCCGTT TGAACGCAAG 
TTTGAAAGCC CTAAATTCAA CCACAATCTC TTGCCGAATG AAACCGCACA CAAACCTGAC 
CTGTCCTCCA AAGCCCCTGC TTCTCCGAAA ACCCTCCTGA AGTCTGCACA GCCTCCTGAG 
TTTGACAGTG GTGTGGAGAC CTTCTCTGTC CACACAGACA ACAAGCTGAA ATACCAAGTA 
AACAGTGTTA GCACGGGGCC GAGAGCAGTG CCCGTGAGTC CCTCAGCCGT GGAGGAAGAT 
GAAGATGAAG ACGGTCACAC AGTGGTGGCT ACTGCCCGCG GCGTGTTTAA CAGCAATGGT 
GGGGTGCTGA GCTCCATTGA GACCGGTGTC AGCATCATCA TCCCCCAGGG AGCCATTCCT 
GAGGGGGTGG AACAGGAGAT CTACTTCAAG GTCTGCCGCG ACAACAGCAT CCTGCCGCCT 
TTGGACAAAG AGAAGGGTGA GACCCTGCTG AGCCCCCTAG TGATGTGTGG GCCCCATGGC 
CTCAAGTTCC TGAAGCCCGT GGAGCTGCGC CTACCACACT GTGCGTCCAT GACTCCTGAC 
GGTTGGTCTT TTGCTCTAAA ATCATCCGAC TCCTCGTCGG GTGATCCTAA AACCTGGCAA 
AACAAGTGTC TTCCTGGAGA TCCAAATTAT CTTGTTGGAG CAAACTGTGT TTCTGTCCTG 
ATTGACCACT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006066459.1
CDS	1..5295
Translation	MTSPLDKENTTMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVNRPDPEPVSENEEDSYDEEAHDPRSGRGALANRRSEKSWGRDRSASRERSLSPRSDRRSVASSQPARPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSADASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTVEEVVVERNEKQAPNLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHPDHQAYPPYSPQAQPQPGHRIDSPGFKTASQQKAEASSPGPYLSPETNPAPSASAVNPHVDLTNVRLEEPSPAPPSSDSPHADSLRAPGTEAAHVQLRGPEPSLSSHVGPAKVYRKDPYPEEMMRQNHVLKQPAAVHPGQRPDKEQGLSYEPQPPYTERQAGRELEQPAYRYDASSYPEQFARNYDHRLRYDDRLLTYEEQWPYYDDKQPYQPRPLDGQPPAEPTERGYFPRFEEPVPLSYEGRAHYDPLPRTSTLRHEEPPAPAPAPGYDVHGRYRPDAQPYSTAGPKVPPEPKPYLDQYPRSYEPAPAQGFPSRAGPGHYEPLHSTTLAPPLGPSSQHKPEVPPTGSKPLPPPPAPAEEEEDPAMKPQSVLTRVKMFENKRSASMENRKDESHSTSFKPSEVTSKPAGAPVPGPKAPAPGQLGEHDRAAHRIPEPQKPHVKPPEDIVRANHYDPEEDEEYYRKQLSYFDRRSFEAKPPTHIPTSHLSEPTRPAHPQSQPQFAGYSSKGKAAEAEAVDRSFGEKRYDPLQATPPPPPLPAQYSQTAQPSPNTALGLHAHTKGAHGEGNAMSLDFQNSLVSKPDPPPSQNKPAAYRPAPREDAVQSAFYPQKSFPEKAPANGVEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPNETAHKPDLSSKAPASPKTLLKSAQPPEFDSGVETFSVHTDNKLKYQVNSVSTGPRAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006066397.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006066397.1

ORF Insert Sequence:

ATGACGTCGC CACTGGACAA GGAGAACACA ACAATGGAGG AAACAGCTAT ATGGGAACAA 
CACACGGTGA CACTTCACAG GGCTCCTGGA TTTGGATTTG GAATTGCAAT ATCTGGTGGA 
AGAGATAATC CACATTTCCA GAGCGGGGAA ACCTCCATCG TGATTTCAGA TGTGCTAAAG 
GGGGGGCCTG CAGAAGGACA GCTGCAGGAA AATGACCGGG TGGCAATGGT TAATGGAGTC 
TCCATGGATA ATGTTGAGCA TGCGTTTGCT GTTCAGCAAC TAAGGAAAAG TGGGAAAAAT 
GCAAAAATTA CCATTCGAAG GAAGAAGAAA GTTCAGATCC CAGTAAATCG TCCTGATCCT 
GAGCCTGTGT CTGAGAACGA AGAGGACAGT TACGACGAGG AGGCGCATGA CCCCAGAAGT 
GGCCGTGGGG CCCTGGCAAA CAGGAGGAGC GAGAAGAGCT GGGGCCGAGA TAGGAGTGCC 
AGTCGGGAGA GGAGTCTGTC CCCGAGGTCT GACAGGCGGT CTGTGGCCTC CAGTCAGCCT 
GCCAGACCCA CCAAAGTCAC CCTGGTGAAG TCCCGGAAGA ATGAAGAATA TGGTCTTCGA 
TTGGCCAGCC ATATATTTGT GAAGGAAATT TCACAAGATA GTTTGGCAGC AAGAGATGGC 
AATATTCAAG AAGGCGATGT TGTATTGAAG ATAAATGGTA CCGTGACGGA AAACATGTCA 
CTGACAGATG CGAAGACGCT GATCGAGAGG TCTAAGGGCA AGTTAAAGAT GGTGGTTCAG 
AGAGATGAGC GGGCTACCCT GCTGAATGTC CCTGACCTTT CTGACAGCAT CCACTCTGCT 
GACGCCTCCG AGAGGGACGA TATTTCAGAA ATCCAGTCAC TGGCATCAGA CCATTCGGGT 
CGATCCCACG ACCGGCCTCC CCGTCACAGC CGCTCTCGGT CTCCTGACCA GCGGTCCGAG 
CCTTCTGACC ATTCTAGACA CTCTCCACAG CAGCCCAGCA GTGGCAGTCT CCGGAGCAGA 
GAGGAAGAGA GAATTTCTAA ACCAGGGGCT GTCTCGACTC CGGTAAAGCA TGCAGATGAT 
CACACACCTA AAACAGTGGA AGAAGTTGTA GTTGAGCGAA ATGAGAAACA AGCACCCAAT 
CTTCCAGAAC CAAAGCCTGT GTATGCCCAG GTAGGACAGC CAGACGTGGA TTTACCTGTC 
AGTCCATCTG ATGGTGTCCT GCCCAACTCA ACTCATGAAG ATGGCATTCT TCGGCCCAGC 
ATGAAGTTAG TAAAATTCAG GAAAGGAGAT AGTGTGGGTC TTCGGCTAGC TGGTGGAAAC 
GATGTTGGCA TATTTGTAGC TGGTGTTCTC GAAGACAGCC CTGCTGCCAA AGAAGGCTTG 
GAGGAGGGCG ACCAGATCCT CAGGGTAAAC AATGTAGATT TCACAAATAT CATAAGAGAA 
GAAGCCGTCC TCTTCCTGCT TGACCTCCCA AAAGGAGAAG AAGTGACCAT ATTGGCTCAG 
AAGAAAAAAG ATGTTTATCG TCGCATCGTA GAATCCGATG TGGGTGATTC TTTCTATATC 
AGAACCCATT TTGAATATGA GAAGGAATCT CCCTATGGAC TCAGTTTTAA CAAAGGAGAG 
GTGTTCCGTG TCGTGGACAC TCTGTACAAT GGGAAGCTGG GGTCCTGGCT CGCCATTCGG 
ATTGGCAAAA ACCATAAAGA GGTGGAACGT GGCATCATCC CAAACAAAAA CAGAGCTGAG 
CAGTTAGCCA GTGTGCAGTA TACACTTCCC AAGACAGCAG GGGGAGACCG CGCTGACTTC 
TGGAGGTTCC GCGGTCTGCG CAGCTCCAAG AGAAACCTGC GCAAGAGCCG AGAAGACCTC 
TCAGCCCAGC CAGTCCAGAC CAAGTTCCCG GCGTATGAGA GGGTGGTTCT TCGAGAAGCT 
GGATTCCTGA GGCCTGTAAC CATCTTTGGA CCAATAGCTG ATGTTGCCAG GGAGAAGCTG 
GCAAGAGAGG AGCCAGATAT TTACCAGATC GCAAAAAGTG AACCTCGGGA TGCAGGAACC 
GACCAGCGCA GCTCTGGCAT TATTCGCCTC CACACAATAA AGCAGATAAT AGATCAGGAC 
AAACATGCCT TGCTGGATGT AACGCCCAAT GCAGTAGACC GTCTGAACTA TGCCCAGTGG 
TATCCAATAG TGGTGTTCCT CAACCCGGAC TCAAAGCAGG GTGTGAAAAC AATGCGGATG 
AGGTTATGTC CAGAATCTCG GAAAAGCGCC AGGAAGTTAT ATGAGCGCTC TCATAAACTT 
CGTAAAAATA ATCACCATCT TTTTACAACT ACAATTAACT TAAATTCAAT GAATGATGGC 
TGGTATGGTG CATTGAAAGA AGCAATCCAG CAACAACAGA ACCAGTTGGT ATGGGTTTCT 
GAGGGAAAGG CGGATGGTGC TACAAGTGAT GACCTTGATT TGCATGATGA TCGTCTGTCC 
TACCTGTCAG CCCCAGGTAG TGAATACTCA ATGTATAGCA CGGACAGTAG ACACACTTCT 
GACTATGAAG ACACAGATAC AGAAGGCGGG GCCTACACTG ATCAAGAACT AGATGAGACC 
CTAAATGATG AGGTGGGGAC TCCCCCGGAG TCTGCCATTA CCCGGTCCTC TGAGCCTGTC 
AGAGAGGACT CCTCTGGAAT GCATCCTGAC CACCAGGCAT ATCCTCCCTA CTCACCACAA 
GCGCAGCCAC AGCCAGGCCA TAGAATAGAC TCCCCTGGAT TTAAAACAGC CTCTCAACAG 
AAAGCAGAAG CCTCATCTCC AGGCCCTTAC CTTTCGCCTG AAACAAACCC GGCACCATCA 
GCCTCTGCTG TTAATCCTCA CGTAGACTTA ACTAATGTCA GACTGGAGGA GCCGAGCCCA 
GCTCCCCCTT CCTCTGACTC ACCACACGCC GACTCTTTAC GAGCTCCAGG CACTGAGGCC 
GCTCACGTGC AGCTCAGAGG TCCAGAGCCG TCCCTGTCGT CGCATGTGGG CCCAGCAAAG 
GTGTACAGGA AGGACCCCTA CCCTGAGGAG ATGATGAGAC AGAACCACGT CTTGAAGCAG 
CCGGCTGCGG TTCACCCTGG GCAGAGGCCA GACAAGGAGC AAGGCCTGAG CTACGAGCCG 
CAGCCTCCGT ACACAGAGCG ACAGGCTGGC AGGGAGTTGG AGCAGCCTGC ATACAGATAT 
GATGCCTCTA GCTACCCGGA GCAGTTTGCT CGGAACTATG ACCATCGTCT GCGCTATGAT 
GACCGCCTCC TCACCTACGA GGAGCAGTGG CCCTACTACG ACGACAAGCA GCCTTACCAG 
CCCCGGCCTC TTGATGGTCA GCCTCCCGCG GAGCCAACAG AACGAGGGTA TTTTCCCCGT 
TTTGAGGAGC CAGTGCCCCT GTCCTATGAA GGCCGAGCGC ACTATGACCC ACTGCCCAGG 
ACCTCCACCT TGCGGCACGA AGAGCCTCCT GCCCCTGCCC CTGCCCCTGG GTACGACGTG 
CACGGCAGGT ACCGGCCTGA CGCGCAGCCC TACTCCACTG CTGGCCCCAA GGTGCCCCCT 
GAGCCCAAGC CGTACTTGGA CCAGTACCCT CGGAGTTACG AGCCAGCGCC AGCCCAGGGC 
TTCCCCTCCA GGGCAGGCCC AGGCCACTAC GAGCCTCTTC ACAGCACCAC GCTCGCTCCA 
CCACTTGGCC CCTCGTCCCA GCACAAGCCC GAAGTCCCGC CCACAGGCTC CAAACCCCTG 
CCTCCACCCC CAGCTCCAGC TGAGGAGGAG GAAGACCCAG CCATGAAGCC ACAATCCGTG 
CTCACCAGAG TGAAGATGTT TGAGAACAAG AGGTCTGCTT CCATGGAGAA CAGGAAGGAT 
GAGAGCCATT CCACTAGTTT CAAGCCCTCA GAAGTCACGT CAAAACCGGC AGGTGCTCCT 
GTCCCAGGTC CCAAAGCCCC TGCTCCGGGT CAGCTCGGTG AACACGACAG AGCAGCGCAT 
AGGATTCCTG AACCACAGAA GCCTCACGTG AAGCCACCTG AAGACATTGT TCGGGCAAAT 
CACTATGACC CCGAGGAGGA TGAGGAGTAC TACCGGAAGC AGCTCTCCTA CTTCGATCGG 
AGAAGCTTTG AGGCCAAGCC CCCCACCCAC ATCCCCACTA GCCACCTCTC CGAGCCCACC 
AGGCCGGCTC ACCCCCAATC TCAGCCACAG TTTGCCGGTT ACTCTTCCAA GGGGAAGGCT 
GCTGAGGCCG AAGCTGTGGA CAGGTCATTC GGGGAGAAGC GCTATGACCC GCTGCAGGCG 
ACGCCCCCCC CTCCGCCGCT GCCCGCTCAG TACAGCCAGA CTGCTCAACC CAGCCCCAAC 
ACGGCTCTCG GCCTCCACGC ACACACCAAG GGGGCGCACG GTGAAGGTAA TGCCATGTCA 
TTGGATTTTC AGAACTCGTT AGTGTCCAAA CCAGACCCAC CTCCATCTCA GAATAAGCCA 
GCAGCTTACA GACCAGCCCC TCGAGAGGAT GCTGTCCAGT CTGCCTTCTA CCCACAAAAG 
AGTTTCCCAG AGAAAGCCCC AGCTAATGGG GTCGAGCAGA CGCAGAAAAC CGTCACGCCA 
GCATACAATC GATTCACACC AAAACCATAC ACAAGCTCTG CACGGCCGTT TGAACGCAAG 
TTTGAAAGCC CTAAATTCAA CCACAATCTC TTGCCGAATG AAACCGCACA CAAACCTGAC 
CTGTCCTCCA AAGCCCCTGC TTCTCCGAAA ACCCTCCTGA AGTCTGCACA GCCTCCTGAG 
TTTGACAGTG GTGTGGAGAC CTTCTCTGTC CACACAGACA ACAAGCTGAA ATACCAAGTA 
AACAGTGTTA GCACGGGGCC GAGAGCAGTG CCCGTGAGTC CCTCAGCCGT GGAGGAAGAT 
GAAGATGAAG ACGGTCACAC AGTGGTGGCT ACTGCCCGCG GCGTGTTTAA CAGCAATGGT 
GGGGTGCTGA GCTCCATTGA GACCGGTGTC AGCATCATCA TCCCCCAGGG AGCCATTCCT 
GAGGGGGTGG AACAGGAGAT CTACTTCAAG GTCTGCCGCG ACAACAGCAT CCTGCCGCCT 
TTGGACAAAG AGAAGGGTGA GACCCTGCTG AGCCCCCTAG TGATGTGTGG GCCCCATGGC 
CTCAAGTTCC TGAAGCCCGT GGAGCTGCGC CTACCACACT GTGCGTCCAT GACTCCTGAC 
GGTTGGTCTT TTGCTCTAAA ATCATCCGAC TCCTCGTCGG GTGATCCTAA AACCTGGCAA 
AACAAGTGTC TTCCTGGAGA TCCAAATTAT CTTGTTGGAG CAAACTGTGT TTCTGTCCTG 
ATTGACCACT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_006066397.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006066459.1 Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X6, mRNA.

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CloneID	OBu21759
Clone ID Related Accession (Same CDS sequence)	XM_006066397.1 , XM_006066397.2
Accession Version	XM_006066397.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	tight junction protein ZO-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006066397.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTCGC CACTGGACAA GGAGAACACA ACAATGGAGG AAACAGCTAT ATGGGAACAA 
CACACGGTGA CACTTCACAG GGCTCCTGGA TTTGGATTTG GAATTGCAAT ATCTGGTGGA 
AGAGATAATC CACATTTCCA GAGCGGGGAA ACCTCCATCG TGATTTCAGA TGTGCTAAAG 
GGGGGGCCTG CAGAAGGACA GCTGCAGGAA AATGACCGGG TGGCAATGGT TAATGGAGTC 
TCCATGGATA ATGTTGAGCA TGCGTTTGCT GTTCAGCAAC TAAGGAAAAG TGGGAAAAAT 
GCAAAAATTA CCATTCGAAG GAAGAAGAAA GTTCAGATCC CAGTAAATCG TCCTGATCCT 
GAGCCTGTGT CTGAGAACGA AGAGGACAGT TACGACGAGG AGGCGCATGA CCCCAGAAGT 
GGCCGTGGGG CCCTGGCAAA CAGGAGGAGC GAGAAGAGCT GGGGCCGAGA TAGGAGTGCC 
AGTCGGGAGA GGAGTCTGTC CCCGAGGTCT GACAGGCGGT CTGTGGCCTC CAGTCAGCCT 
GCCAGACCCA CCAAAGTCAC CCTGGTGAAG TCCCGGAAGA ATGAAGAATA TGGTCTTCGA 
TTGGCCAGCC ATATATTTGT GAAGGAAATT TCACAAGATA GTTTGGCAGC AAGAGATGGC 
AATATTCAAG AAGGCGATGT TGTATTGAAG ATAAATGGTA CCGTGACGGA AAACATGTCA 
CTGACAGATG CGAAGACGCT GATCGAGAGG TCTAAGGGCA AGTTAAAGAT GGTGGTTCAG 
AGAGATGAGC GGGCTACCCT GCTGAATGTC CCTGACCTTT CTGACAGCAT CCACTCTGCT 
GACGCCTCCG AGAGGGACGA TATTTCAGAA ATCCAGTCAC TGGCATCAGA CCATTCGGGT 
CGATCCCACG ACCGGCCTCC CCGTCACAGC CGCTCTCGGT CTCCTGACCA GCGGTCCGAG 
CCTTCTGACC ATTCTAGACA CTCTCCACAG CAGCCCAGCA GTGGCAGTCT CCGGAGCAGA 
GAGGAAGAGA GAATTTCTAA ACCAGGGGCT GTCTCGACTC CGGTAAAGCA TGCAGATGAT 
CACACACCTA AAACAGTGGA AGAAGTTGTA GTTGAGCGAA ATGAGAAACA AGCACCCAAT 
CTTCCAGAAC CAAAGCCTGT GTATGCCCAG GTAGGACAGC CAGACGTGGA TTTACCTGTC 
AGTCCATCTG ATGGTGTCCT GCCCAACTCA ACTCATGAAG ATGGCATTCT TCGGCCCAGC 
ATGAAGTTAG TAAAATTCAG GAAAGGAGAT AGTGTGGGTC TTCGGCTAGC TGGTGGAAAC 
GATGTTGGCA TATTTGTAGC TGGTGTTCTC GAAGACAGCC CTGCTGCCAA AGAAGGCTTG 
GAGGAGGGCG ACCAGATCCT CAGGGTAAAC AATGTAGATT TCACAAATAT CATAAGAGAA 
GAAGCCGTCC TCTTCCTGCT TGACCTCCCA AAAGGAGAAG AAGTGACCAT ATTGGCTCAG 
AAGAAAAAAG ATGTTTATCG TCGCATCGTA GAATCCGATG TGGGTGATTC TTTCTATATC 
AGAACCCATT TTGAATATGA GAAGGAATCT CCCTATGGAC TCAGTTTTAA CAAAGGAGAG 
GTGTTCCGTG TCGTGGACAC TCTGTACAAT GGGAAGCTGG GGTCCTGGCT CGCCATTCGG 
ATTGGCAAAA ACCATAAAGA GGTGGAACGT GGCATCATCC CAAACAAAAA CAGAGCTGAG 
CAGTTAGCCA GTGTGCAGTA TACACTTCCC AAGACAGCAG GGGGAGACCG CGCTGACTTC 
TGGAGGTTCC GCGGTCTGCG CAGCTCCAAG AGAAACCTGC GCAAGAGCCG AGAAGACCTC 
TCAGCCCAGC CAGTCCAGAC CAAGTTCCCG GCGTATGAGA GGGTGGTTCT TCGAGAAGCT 
GGATTCCTGA GGCCTGTAAC CATCTTTGGA CCAATAGCTG ATGTTGCCAG GGAGAAGCTG 
GCAAGAGAGG AGCCAGATAT TTACCAGATC GCAAAAAGTG AACCTCGGGA TGCAGGAACC 
GACCAGCGCA GCTCTGGCAT TATTCGCCTC CACACAATAA AGCAGATAAT AGATCAGGAC 
AAACATGCCT TGCTGGATGT AACGCCCAAT GCAGTAGACC GTCTGAACTA TGCCCAGTGG 
TATCCAATAG TGGTGTTCCT CAACCCGGAC TCAAAGCAGG GTGTGAAAAC AATGCGGATG 
AGGTTATGTC CAGAATCTCG GAAAAGCGCC AGGAAGTTAT ATGAGCGCTC TCATAAACTT 
CGTAAAAATA ATCACCATCT TTTTACAACT ACAATTAACT TAAATTCAAT GAATGATGGC 
TGGTATGGTG CATTGAAAGA AGCAATCCAG CAACAACAGA ACCAGTTGGT ATGGGTTTCT 
GAGGGAAAGG CGGATGGTGC TACAAGTGAT GACCTTGATT TGCATGATGA TCGTCTGTCC 
TACCTGTCAG CCCCAGGTAG TGAATACTCA ATGTATAGCA CGGACAGTAG ACACACTTCT 
GACTATGAAG ACACAGATAC AGAAGGCGGG GCCTACACTG ATCAAGAACT AGATGAGACC 
CTAAATGATG AGGTGGGGAC TCCCCCGGAG TCTGCCATTA CCCGGTCCTC TGAGCCTGTC 
AGAGAGGACT CCTCTGGAAT GCATCCTGAC CACCAGGCAT ATCCTCCCTA CTCACCACAA 
GCGCAGCCAC AGCCAGGCCA TAGAATAGAC TCCCCTGGAT TTAAAACAGC CTCTCAACAG 
AAAGCAGAAG CCTCATCTCC AGGCCCTTAC CTTTCGCCTG AAACAAACCC GGCACCATCA 
GCCTCTGCTG TTAATCCTCA CGTAGACTTA ACTAATGTCA GACTGGAGGA GCCGAGCCCA 
GCTCCCCCTT CCTCTGACTC ACCACACGCC GACTCTTTAC GAGCTCCAGG CACTGAGGCC 
GCTCACGTGC AGCTCAGAGG TCCAGAGCCG TCCCTGTCGT CGCATGTGGG CCCAGCAAAG 
GTGTACAGGA AGGACCCCTA CCCTGAGGAG ATGATGAGAC AGAACCACGT CTTGAAGCAG 
CCGGCTGCGG TTCACCCTGG GCAGAGGCCA GACAAGGAGC AAGGCCTGAG CTACGAGCCG 
CAGCCTCCGT ACACAGAGCG ACAGGCTGGC AGGGAGTTGG AGCAGCCTGC ATACAGATAT 
GATGCCTCTA GCTACCCGGA GCAGTTTGCT CGGAACTATG ACCATCGTCT GCGCTATGAT 
GACCGCCTCC TCACCTACGA GGAGCAGTGG CCCTACTACG ACGACAAGCA GCCTTACCAG 
CCCCGGCCTC TTGATGGTCA GCCTCCCGCG GAGCCAACAG AACGAGGGTA TTTTCCCCGT 
TTTGAGGAGC CAGTGCCCCT GTCCTATGAA GGCCGAGCGC ACTATGACCC ACTGCCCAGG 
ACCTCCACCT TGCGGCACGA AGAGCCTCCT GCCCCTGCCC CTGCCCCTGG GTACGACGTG 
CACGGCAGGT ACCGGCCTGA CGCGCAGCCC TACTCCACTG CTGGCCCCAA GGTGCCCCCT 
GAGCCCAAGC CGTACTTGGA CCAGTACCCT CGGAGTTACG AGCCAGCGCC AGCCCAGGGC 
TTCCCCTCCA GGGCAGGCCC AGGCCACTAC GAGCCTCTTC ACAGCACCAC GCTCGCTCCA 
CCACTTGGCC CCTCGTCCCA GCACAAGCCC GAAGTCCCGC CCACAGGCTC CAAACCCCTG 
CCTCCACCCC CAGCTCCAGC TGAGGAGGAG GAAGACCCAG CCATGAAGCC ACAATCCGTG 
CTCACCAGAG TGAAGATGTT TGAGAACAAG AGGTCTGCTT CCATGGAGAA CAGGAAGGAT 
GAGAGCCATT CCACTAGTTT CAAGCCCTCA GAAGTCACGT CAAAACCGGC AGGTGCTCCT 
GTCCCAGGTC CCAAAGCCCC TGCTCCGGGT CAGCTCGGTG AACACGACAG AGCAGCGCAT 
AGGATTCCTG AACCACAGAA GCCTCACGTG AAGCCACCTG AAGACATTGT TCGGGCAAAT 
CACTATGACC CCGAGGAGGA TGAGGAGTAC TACCGGAAGC AGCTCTCCTA CTTCGATCGG 
AGAAGCTTTG AGGCCAAGCC CCCCACCCAC ATCCCCACTA GCCACCTCTC CGAGCCCACC 
AGGCCGGCTC ACCCCCAATC TCAGCCACAG TTTGCCGGTT ACTCTTCCAA GGGGAAGGCT 
GCTGAGGCCG AAGCTGTGGA CAGGTCATTC GGGGAGAAGC GCTATGACCC GCTGCAGGCG 
ACGCCCCCCC CTCCGCCGCT GCCCGCTCAG TACAGCCAGA CTGCTCAACC CAGCCCCAAC 
ACGGCTCTCG GCCTCCACGC ACACACCAAG GGGGCGCACG GTGAAGGTAA TGCCATGTCA 
TTGGATTTTC AGAACTCGTT AGTGTCCAAA CCAGACCCAC CTCCATCTCA GAATAAGCCA 
GCAGCTTACA GACCAGCCCC TCGAGAGGAT GCTGTCCAGT CTGCCTTCTA CCCACAAAAG 
AGTTTCCCAG AGAAAGCCCC AGCTAATGGG GTCGAGCAGA CGCAGAAAAC CGTCACGCCA 
GCATACAATC GATTCACACC AAAACCATAC ACAAGCTCTG CACGGCCGTT TGAACGCAAG 
TTTGAAAGCC CTAAATTCAA CCACAATCTC TTGCCGAATG AAACCGCACA CAAACCTGAC 
CTGTCCTCCA AAGCCCCTGC TTCTCCGAAA ACCCTCCTGA AGTCTGCACA GCCTCCTGAG 
TTTGACAGTG GTGTGGAGAC CTTCTCTGTC CACACAGACA ACAAGCTGAA ATACCAAGTA 
AACAGTGTTA GCACGGGGCC GAGAGCAGTG CCCGTGAGTC CCTCAGCCGT GGAGGAAGAT 
GAAGATGAAG ACGGTCACAC AGTGGTGGCT ACTGCCCGCG GCGTGTTTAA CAGCAATGGT 
GGGGTGCTGA GCTCCATTGA GACCGGTGTC AGCATCATCA TCCCCCAGGG AGCCATTCCT 
GAGGGGGTGG AACAGGAGAT CTACTTCAAG GTCTGCCGCG ACAACAGCAT CCTGCCGCCT 
TTGGACAAAG AGAAGGGTGA GACCCTGCTG AGCCCCCTAG TGATGTGTGG GCCCCATGGC 
CTCAAGTTCC TGAAGCCCGT GGAGCTGCGC CTACCACACT GTGCGTCCAT GACTCCTGAC 
GGTTGGTCTT TTGCTCTAAA ATCATCCGAC TCCTCGTCGG GTGATCCTAA AACCTGGCAA 
AACAAGTGTC TTCCTGGAGA TCCAAATTAT CTTGTTGGAG CAAACTGTGT TTCTGTCCTG 
ATTGACCACT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006066459.1
CDS	16..5310
Translation	MTSPLDKENTTMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVNRPDPEPVSENEEDSYDEEAHDPRSGRGALANRRSEKSWGRDRSASRERSLSPRSDRRSVASSQPARPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSADASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTVEEVVVERNEKQAPNLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHPDHQAYPPYSPQAQPQPGHRIDSPGFKTASQQKAEASSPGPYLSPETNPAPSASAVNPHVDLTNVRLEEPSPAPPSSDSPHADSLRAPGTEAAHVQLRGPEPSLSSHVGPAKVYRKDPYPEEMMRQNHVLKQPAAVHPGQRPDKEQGLSYEPQPPYTERQAGRELEQPAYRYDASSYPEQFARNYDHRLRYDDRLLTYEEQWPYYDDKQPYQPRPLDGQPPAEPTERGYFPRFEEPVPLSYEGRAHYDPLPRTSTLRHEEPPAPAPAPGYDVHGRYRPDAQPYSTAGPKVPPEPKPYLDQYPRSYEPAPAQGFPSRAGPGHYEPLHSTTLAPPLGPSSQHKPEVPPTGSKPLPPPPAPAEEEEDPAMKPQSVLTRVKMFENKRSASMENRKDESHSTSFKPSEVTSKPAGAPVPGPKAPAPGQLGEHDRAAHRIPEPQKPHVKPPEDIVRANHYDPEEDEEYYRKQLSYFDRRSFEAKPPTHIPTSHLSEPTRPAHPQSQPQFAGYSSKGKAAEAEAVDRSFGEKRYDPLQATPPPPPLPAQYSQTAQPSPNTALGLHAHTKGAHGEGNAMSLDFQNSLVSKPDPPPSQNKPAAYRPAPREDAVQSAFYPQKSFPEKAPANGVEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPNETAHKPDLSSKAPASPKTLLKSAQPPEFDSGVETFSVHTDNKLKYQVNSVSTGPRAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006066397.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006066397.2

ORF Insert Sequence:

ATGACGTCGC CACTGGACAA GGAGAACACA ACAATGGAGG AAACAGCTAT ATGGGAACAA 
CACACGGTGA CACTTCACAG GGCTCCTGGA TTTGGATTTG GAATTGCAAT ATCTGGTGGA 
AGAGATAATC CACATTTCCA GAGCGGGGAA ACCTCCATCG TGATTTCAGA TGTGCTAAAG 
GGGGGGCCTG CAGAAGGACA GCTGCAGGAA AATGACCGGG TGGCAATGGT TAATGGAGTC 
TCCATGGATA ATGTTGAGCA TGCGTTTGCT GTTCAGCAAC TAAGGAAAAG TGGGAAAAAT 
GCAAAAATTA CCATTCGAAG GAAGAAGAAA GTTCAGATCC CAGTAAATCG TCCTGATCCT 
GAGCCTGTGT CTGAGAACGA AGAGGACAGT TACGACGAGG AGGCGCATGA CCCCAGAAGT 
GGCCGTGGGG CCCTGGCAAA CAGGAGGAGC GAGAAGAGCT GGGGCCGAGA TAGGAGTGCC 
AGTCGGGAGA GGAGTCTGTC CCCGAGGTCT GACAGGCGGT CTGTGGCCTC CAGTCAGCCT 
GCCAGACCCA CCAAAGTCAC CCTGGTGAAG TCCCGGAAGA ATGAAGAATA TGGTCTTCGA 
TTGGCCAGCC ATATATTTGT GAAGGAAATT TCACAAGATA GTTTGGCAGC AAGAGATGGC 
AATATTCAAG AAGGCGATGT TGTATTGAAG ATAAATGGTA CCGTGACGGA AAACATGTCA 
CTGACAGATG CGAAGACGCT GATCGAGAGG TCTAAGGGCA AGTTAAAGAT GGTGGTTCAG 
AGAGATGAGC GGGCTACCCT GCTGAATGTC CCTGACCTTT CTGACAGCAT CCACTCTGCT 
GACGCCTCCG AGAGGGACGA TATTTCAGAA ATCCAGTCAC TGGCATCAGA CCATTCGGGT 
CGATCCCACG ACCGGCCTCC CCGTCACAGC CGCTCTCGGT CTCCTGACCA GCGGTCCGAG 
CCTTCTGACC ATTCTAGACA CTCTCCACAG CAGCCCAGCA GTGGCAGTCT CCGGAGCAGA 
GAGGAAGAGA GAATTTCTAA ACCAGGGGCT GTCTCGACTC CGGTAAAGCA TGCAGATGAT 
CACACACCTA AAACAGTGGA AGAAGTTGTA GTTGAGCGAA ATGAGAAACA AGCACCCAAT 
CTTCCAGAAC CAAAGCCTGT GTATGCCCAG GTAGGACAGC CAGACGTGGA TTTACCTGTC 
AGTCCATCTG ATGGTGTCCT GCCCAACTCA ACTCATGAAG ATGGCATTCT TCGGCCCAGC 
ATGAAGTTAG TAAAATTCAG GAAAGGAGAT AGTGTGGGTC TTCGGCTAGC TGGTGGAAAC 
GATGTTGGCA TATTTGTAGC TGGTGTTCTC GAAGACAGCC CTGCTGCCAA AGAAGGCTTG 
GAGGAGGGCG ACCAGATCCT CAGGGTAAAC AATGTAGATT TCACAAATAT CATAAGAGAA 
GAAGCCGTCC TCTTCCTGCT TGACCTCCCA AAAGGAGAAG AAGTGACCAT ATTGGCTCAG 
AAGAAAAAAG ATGTTTATCG TCGCATCGTA GAATCCGATG TGGGTGATTC TTTCTATATC 
AGAACCCATT TTGAATATGA GAAGGAATCT CCCTATGGAC TCAGTTTTAA CAAAGGAGAG 
GTGTTCCGTG TCGTGGACAC TCTGTACAAT GGGAAGCTGG GGTCCTGGCT CGCCATTCGG 
ATTGGCAAAA ACCATAAAGA GGTGGAACGT GGCATCATCC CAAACAAAAA CAGAGCTGAG 
CAGTTAGCCA GTGTGCAGTA TACACTTCCC AAGACAGCAG GGGGAGACCG CGCTGACTTC 
TGGAGGTTCC GCGGTCTGCG CAGCTCCAAG AGAAACCTGC GCAAGAGCCG AGAAGACCTC 
TCAGCCCAGC CAGTCCAGAC CAAGTTCCCG GCGTATGAGA GGGTGGTTCT TCGAGAAGCT 
GGATTCCTGA GGCCTGTAAC CATCTTTGGA CCAATAGCTG ATGTTGCCAG GGAGAAGCTG 
GCAAGAGAGG AGCCAGATAT TTACCAGATC GCAAAAAGTG AACCTCGGGA TGCAGGAACC 
GACCAGCGCA GCTCTGGCAT TATTCGCCTC CACACAATAA AGCAGATAAT AGATCAGGAC 
AAACATGCCT TGCTGGATGT AACGCCCAAT GCAGTAGACC GTCTGAACTA TGCCCAGTGG 
TATCCAATAG TGGTGTTCCT CAACCCGGAC TCAAAGCAGG GTGTGAAAAC AATGCGGATG 
AGGTTATGTC CAGAATCTCG GAAAAGCGCC AGGAAGTTAT ATGAGCGCTC TCATAAACTT 
CGTAAAAATA ATCACCATCT TTTTACAACT ACAATTAACT TAAATTCAAT GAATGATGGC 
TGGTATGGTG CATTGAAAGA AGCAATCCAG CAACAACAGA ACCAGTTGGT ATGGGTTTCT 
GAGGGAAAGG CGGATGGTGC TACAAGTGAT GACCTTGATT TGCATGATGA TCGTCTGTCC 
TACCTGTCAG CCCCAGGTAG TGAATACTCA ATGTATAGCA CGGACAGTAG ACACACTTCT 
GACTATGAAG ACACAGATAC AGAAGGCGGG GCCTACACTG ATCAAGAACT AGATGAGACC 
CTAAATGATG AGGTGGGGAC TCCCCCGGAG TCTGCCATTA CCCGGTCCTC TGAGCCTGTC 
AGAGAGGACT CCTCTGGAAT GCATCCTGAC CACCAGGCAT ATCCTCCCTA CTCACCACAA 
GCGCAGCCAC AGCCAGGCCA TAGAATAGAC TCCCCTGGAT TTAAAACAGC CTCTCAACAG 
AAAGCAGAAG CCTCATCTCC AGGCCCTTAC CTTTCGCCTG AAACAAACCC GGCACCATCA 
GCCTCTGCTG TTAATCCTCA CGTAGACTTA ACTAATGTCA GACTGGAGGA GCCGAGCCCA 
GCTCCCCCTT CCTCTGACTC ACCACACGCC GACTCTTTAC GAGCTCCAGG CACTGAGGCC 
GCTCACGTGC AGCTCAGAGG TCCAGAGCCG TCCCTGTCGT CGCATGTGGG CCCAGCAAAG 
GTGTACAGGA AGGACCCCTA CCCTGAGGAG ATGATGAGAC AGAACCACGT CTTGAAGCAG 
CCGGCTGCGG TTCACCCTGG GCAGAGGCCA GACAAGGAGC AAGGCCTGAG CTACGAGCCG 
CAGCCTCCGT ACACAGAGCG ACAGGCTGGC AGGGAGTTGG AGCAGCCTGC ATACAGATAT 
GATGCCTCTA GCTACCCGGA GCAGTTTGCT CGGAACTATG ACCATCGTCT GCGCTATGAT 
GACCGCCTCC TCACCTACGA GGAGCAGTGG CCCTACTACG ACGACAAGCA GCCTTACCAG 
CCCCGGCCTC TTGATGGTCA GCCTCCCGCG GAGCCAACAG AACGAGGGTA TTTTCCCCGT 
TTTGAGGAGC CAGTGCCCCT GTCCTATGAA GGCCGAGCGC ACTATGACCC ACTGCCCAGG 
ACCTCCACCT TGCGGCACGA AGAGCCTCCT GCCCCTGCCC CTGCCCCTGG GTACGACGTG 
CACGGCAGGT ACCGGCCTGA CGCGCAGCCC TACTCCACTG CTGGCCCCAA GGTGCCCCCT 
GAGCCCAAGC CGTACTTGGA CCAGTACCCT CGGAGTTACG AGCCAGCGCC AGCCCAGGGC 
TTCCCCTCCA GGGCAGGCCC AGGCCACTAC GAGCCTCTTC ACAGCACCAC GCTCGCTCCA 
CCACTTGGCC CCTCGTCCCA GCACAAGCCC GAAGTCCCGC CCACAGGCTC CAAACCCCTG 
CCTCCACCCC CAGCTCCAGC TGAGGAGGAG GAAGACCCAG CCATGAAGCC ACAATCCGTG 
CTCACCAGAG TGAAGATGTT TGAGAACAAG AGGTCTGCTT CCATGGAGAA CAGGAAGGAT 
GAGAGCCATT CCACTAGTTT CAAGCCCTCA GAAGTCACGT CAAAACCGGC AGGTGCTCCT 
GTCCCAGGTC CCAAAGCCCC TGCTCCGGGT CAGCTCGGTG AACACGACAG AGCAGCGCAT 
AGGATTCCTG AACCACAGAA GCCTCACGTG AAGCCACCTG AAGACATTGT TCGGGCAAAT 
CACTATGACC CCGAGGAGGA TGAGGAGTAC TACCGGAAGC AGCTCTCCTA CTTCGATCGG 
AGAAGCTTTG AGGCCAAGCC CCCCACCCAC ATCCCCACTA GCCACCTCTC CGAGCCCACC 
AGGCCGGCTC ACCCCCAATC TCAGCCACAG TTTGCCGGTT ACTCTTCCAA GGGGAAGGCT 
GCTGAGGCCG AAGCTGTGGA CAGGTCATTC GGGGAGAAGC GCTATGACCC GCTGCAGGCG 
ACGCCCCCCC CTCCGCCGCT GCCCGCTCAG TACAGCCAGA CTGCTCAACC CAGCCCCAAC 
ACGGCTCTCG GCCTCCACGC ACACACCAAG GGGGCGCACG GTGAAGGTAA TGCCATGTCA 
TTGGATTTTC AGAACTCGTT AGTGTCCAAA CCAGACCCAC CTCCATCTCA GAATAAGCCA 
GCAGCTTACA GACCAGCCCC TCGAGAGGAT GCTGTCCAGT CTGCCTTCTA CCCACAAAAG 
AGTTTCCCAG AGAAAGCCCC AGCTAATGGG GTCGAGCAGA CGCAGAAAAC CGTCACGCCA 
GCATACAATC GATTCACACC AAAACCATAC ACAAGCTCTG CACGGCCGTT TGAACGCAAG 
TTTGAAAGCC CTAAATTCAA CCACAATCTC TTGCCGAATG AAACCGCACA CAAACCTGAC 
CTGTCCTCCA AAGCCCCTGC TTCTCCGAAA ACCCTCCTGA AGTCTGCACA GCCTCCTGAG 
TTTGACAGTG GTGTGGAGAC CTTCTCTGTC CACACAGACA ACAAGCTGAA ATACCAAGTA 
AACAGTGTTA GCACGGGGCC GAGAGCAGTG CCCGTGAGTC CCTCAGCCGT GGAGGAAGAT 
GAAGATGAAG ACGGTCACAC AGTGGTGGCT ACTGCCCGCG GCGTGTTTAA CAGCAATGGT 
GGGGTGCTGA GCTCCATTGA GACCGGTGTC AGCATCATCA TCCCCCAGGG AGCCATTCCT 
GAGGGGGTGG AACAGGAGAT CTACTTCAAG GTCTGCCGCG ACAACAGCAT CCTGCCGCCT 
TTGGACAAAG AGAAGGGTGA GACCCTGCTG AGCCCCCTAG TGATGTGTGG GCCCCATGGC 
CTCAAGTTCC TGAAGCCCGT GGAGCTGCGC CTACCACACT GTGCGTCCAT GACTCCTGAC 
GGTTGGTCTT TTGCTCTAAA ATCATCCGAC TCCTCGTCGG GTGATCCTAA AACCTGGCAA 
AACAAGTGTC TTCCTGGAGA TCCAAATTAT CTTGTTGGAG CAAACTGTGT TTCTGTCCTG 
ATTGACCACT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_006066398.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006066460.1 Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X6, mRNA.

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CloneID	OBu21760
Clone ID Related Accession (Same CDS sequence)	XM_006066398.1
Accession Version	XM_006066398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	tight junction protein ZO-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785206.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACATG CTGCGTATTT TATCTCTCTT CGCCCCCGCC GCCCCATGGT AACGCCGTCC 
TCTGAAAACC CCAGTGGTGC TACTTCTAGT GTCAGCCAAG GAAAGCCTTC TTTAAGACGA 
ATTAAAGGGA GACTGCACAG GAGCAAGAGC CTCGATAGCA TCGATTTCTG TGAACTCACT 
AACACAACAA TGGAGGAAAC AGCTATATGG GAACAACACA CGGTGACACT TCACAGGGCT 
CCTGGATTTG GATTTGGAAT TGCAATATCT GGTGGAAGAG ATAATCCACA TTTCCAGAGC 
GGGGAAACCT CCATCGTGAT TTCAGATGTG CTAAAGGGGG GGCCTGCAGA AGGACAGCTG 
CAGGAAAATG ACCGGGTGGC AATGGTTAAT GGAGTCTCCA TGGATAATGT TGAGCATGCG 
TTTGCTGTTC AGCAACTAAG GAAAAGTGGG AAAAATGCAA AAATTACCAT TCGAAGGAAG 
AAGAAAGTTC AGATCCCAGT AAATCGTCCT GATCCTGAGC CTGTGTCTGA GAACGAAGAG 
GACAGTTACG ACGAGGAGGC GCATGACCCC AGAAGTGGCC GTGGGGCCCT GGCAAACAGG 
AGGAGCGAGA AGAGCTGGGG CCGAGATAGG AGTGCCAGTC GGGAGAGGAG TCTGTCCCCG 
AGGTCTGACA GGCGGTCTGT GGCCTCCAGT CAGCCTGCCA GACCCACCAA AGTCACCCTG 
GTGAAGTCCC GGAAGAATGA AGAATATGGT CTTCGATTGG CCAGCCATAT ATTTGTGAAG 
GAAATTTCAC AAGATAGTTT GGCAGCAAGA GATGGCAATA TTCAAGAAGG CGATGTTGTA 
TTGAAGATAA ATGGTACCGT GACGGAAAAC ATGTCACTGA CAGATGCGAA GACGCTGATC 
GAGAGGTCTA AGGGCAAGTT AAAGATGGTG GTTCAGAGAG ATGAGCGGGC TACCCTGCTG 
AATGTCCCTG ACCTTTCTGA CAGCATCCAC TCTGCTGACG CCTCCGAGAG GGACGATATT 
TCAGAAATCC AGTCACTGGC ATCAGACCAT TCGGGTCGAT CCCACGACCG GCCTCCCCGT 
CACAGCCGCT CTCGGTCTCC TGACCAGCGG TCCGAGCCTT CTGACCATTC TAGACACTCT 
CCACAGCAGC CCAGCAGTGG CAGTCTCCGG AGCAGAGAGG AAGAGAGAAT TTCTAAACCA 
GGGGCTGTCT CGACTCCGGT AAAGCATGCA GATGATCACA CACCTAAAAC AGTGGAAGAA 
GTTGTAGTTG AGCGAAATGA GAAACAAGCA CCCAATCTTC CAGAACCAAA GCCTGTGTAT 
GCCCAGGTAG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG TGTCCTGCCC 
AACTCAACTC ATGAAGATGG CATTCTTCGG CCCAGCATGA AGTTAGTAAA ATTCAGGAAA 
GGAGATAGTG TGGGTCTTCG GCTAGCTGGT GGAAACGATG TTGGCATATT TGTAGCTGGT 
GTTCTCGAAG ACAGCCCTGC TGCCAAAGAA GGCTTGGAGG AGGGCGACCA GATCCTCAGG 
GTAAACAATG TAGATTTCAC AAATATCATA AGAGAAGAAG CCGTCCTCTT CCTGCTTGAC 
CTCCCAAAAG GAGAAGAAGT GACCATATTG GCTCAGAAGA AAAAAGATGT TTATCGTCGC 
ATCGTAGAAT CCGATGTGGG TGATTCTTTC TATATCAGAA CCCATTTTGA ATATGAGAAG 
GAATCTCCCT ATGGACTCAG TTTTAACAAA GGAGAGGTGT TCCGTGTCGT GGACACTCTG 
TACAATGGGA AGCTGGGGTC CTGGCTCGCC ATTCGGATTG GCAAAAACCA TAAAGAGGTG 
GAACGTGGCA TCATCCCAAA CAAAAACAGA GCTGAGCAGT TAGCCAGTGT GCAGTATACA 
CTTCCCAAGA CAGCAGGGGG AGACCGCGCT GACTTCTGGA GGTTCCGCGG TCTGCGCAGC 
TCCAAGAGAA ACCTGCGCAA GAGCCGAGAA GACCTCTCAG CCCAGCCAGT CCAGACCAAG 
TTCCCGGCGT ATGAGAGGGT GGTTCTTCGA GAAGCTGGAT TCCTGAGGCC TGTAACCATC 
TTTGGACCAA TAGCTGATGT TGCCAGGGAG AAGCTGGCAA GAGAGGAGCC AGATATTTAC 
CAGATCGCAA AAAGTGAACC TCGGGATGCA GGAACCGACC AGCGCAGCTC TGGCATTATT 
CGCCTCCACA CAATAAAGCA GATAATAGAT CAGGACAAAC ATGCCTTGCT GGATGTAACG 
CCCAATGCAG TAGACCGTCT GAACTATGCC CAGTGGTATC CAATAGTGGT GTTCCTCAAC 
CCGGACTCAA AGCAGGGTGT GAAAACAATG CGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGCGCCAGGA AGTTATATGA GCGCTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGCTGGT ATGGTGCATT GAAAGAAGCA 
ATCCAGCAAC AACAGAACCA GTTGGTATGG GTTTCTGAGG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAGACCCTAA ATGATGAGGT GGGGACTCCC 
CCGGAGTCTG CCATTACCCG GTCCTCTGAG CCTGTCAGAG AGGACTCCTC TGGAATGCAT 
CCTGACCACC AGGCATATCC TCCCTACTCA CCACAAGCGC AGCCACAGCC AGGCCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGGTGT ACAGGAAGGA CCCCTACCCT 
GAGGAGATGA TGAGACAGAA CCACGTCTTG AAGCAGCCGG CTGCGGTTCA CCCTGGGCAG 
AGGCCAGACA AGGAGCAAGG CCTGAGCTAC GAGCCGCAGC CTCCGTACAC AGAGCGACAG 
GCTGGCAGGG AGTTGGAGCA GCCTGCATAC AGATATGATG CCTCTAGCTA CCCGGAGCAG 
TTTGCTCGGA ACTATGACCA TCGTCTGCGC TATGATGACC GCCTCCTCAC CTACGAGGAG 
CAGTGGCCCT ACTACGACGA CAAGCAGCCT TACCAGCCCC GGCCTCTTGA TGGTCAGCCT 
CCCGCGGAGC CAACAGAACG AGGGTATTTT CCCCGTTTTG AGGAGCCAGT GCCCCTGTCC 
TATGAAGGCC GAGCGCACTA TGACCCACTG CCCAGGACCT CCACCTTGCG GCACGAAGAG 
CCTCCTGCCC CTGCCCCTGC CCCTGGGTAC GACGTGCACG GCAGGTACCG GCCTGACGCG 
CAGCCCTACT CCACTGCTGG CCCCAAGGTG CCCCCTGAGC CCAAGCCGTA CTTGGACCAG 
TACCCTCGGA GTTACGAGCC AGCGCCAGCC CAGGGCTTCC CCTCCAGGGC AGGCCCAGGC 
CACTACGAGC CTCTTCACAG CACCACGCTC GCTCCACCAC TTGGCCCCTC GTCCCAGCAC 
AAGCCCGAAG TCCCGCCCAC AGGCTCCAAA CCCCTGCCTC CACCCCCAGC TCCAGCTGAG 
GAGGAGGAAG ACCCAGCCAT GAAGCCACAA TCCGTGCTCA CCAGAGTGAA GATGTTTGAG 
AACAAGAGGT CTGCTTCCAT GGAGAACAGG AAGGATGAGA GCCATTCCAC TAGTTTCAAG 
CCCTCAGAAG TCACGTCAAA ACCGGCAGGT GCTCCTGTCC CAGGTCCCAA AGCCCCTGCT 
CCGGGTCAGC TCGGTGAACA CGACAGAGCA GCGCATAGGA TTCCTGAACC ACAGAAGCCT 
CACGTGAAGC CACCTGAAGA CATTGTTCGG GCAAATCACT ATGACCCCGA GGAGGATGAG 
GAGTACTACC GGAAGCAGCT CTCCTACTTC GATCGGAGAA GCTTTGAGGC CAAGCCCCCC 
ACCCACATCC CCACTAGCCA CCTCTCCGAG CCCACCAGGC CGGCTCACCC CCAATCTCAG 
CCACAGTTTG CCGGTTACTC TTCCAAGGGG AAGGCTGCTG AGGCCGAAGC TGTGGACAGG 
TCATTCGGGG AGAAGCGCTA TGACCCGCTG CAGGCGACGC CCCCCCCTCC GCCGCTGCCC 
GCTCAGTACA GCCAGACTGC TCAACCCAGC CCCAACACGG CTCTCGGCCT CCACGCACAC 
ACCAAGGGGG CGCACGGTGA AGGTAATGCC ATGTCATTGG ATTTTCAGAA CTCGTTAGTG 
TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAG CTTACAGACC AGCCCCTCGA 
GAGGATGCTG TCCAGTCTGC CTTCTACCCA CAAAAGAGTT TCCCAGAGAA AGCCCCAGCT 
AATGGGGTCG AGCAGACGCA GAAAACCGTC ACGCCAGCAT ACAATCGATT CACACCAAAA 
CCATACACAA GCTCTGCACG GCCGTTTGAA CGCAAGTTTG AAAGCCCTAA ATTCAACCAC 
AATCTCTTGC CGAATGAAAC CGCACACAAA CCTGACCTGT CCTCCAAAGC CCCTGCTTCT 
CCGAAAACCC TCCTGAAGTC TGCACAGCCT CCTGAGTTTG ACAGTGGTGT GGAGACCTTC 
TCTGTCCACA CAGACAACAA GCTGAAATAC CAAGTAAACA GTGTTAGCAC GGGGCCGAGA 
GCAGTGCCCG TGAGTCCCTC AGCCGTGGAG GAAGATGAAG ATGAAGACGG TCACACAGTG 
GTGGCTACTG CCCGCGGCGT GTTTAACAGC AATGGTGGGG TGCTGAGCTC CATTGAGACC 
GGTGTCAGCA TCATCATCCC CCAGGGAGCC ATTCCTGAGG GGGTGGAACA GGAGATCTAC 
TTCAAGGTCT GCCGCGACAA CAGCATCCTG CCGCCTTTGG ACAAAGAGAA GGGTGAGACC 
CTGCTGAGCC CCCTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCCTAC CACACTGTGC GTCCATGACT CCTGACGGTT GGTCTTTTGC TCTAAAATCA 
TCCGACTCCT CGTCGGGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTATCTTG TTGGAGCAAA CTGTGTTTCT GTCCTGATTG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006066460.1
CDS	1..5211
Translation	MEHAAYFISLRPRRPMVTPSSENPSGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTTMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVNRPDPEPVSENEEDSYDEEAHDPRSGRGALANRRSEKSWGRDRSASRERSLSPRSDRRSVASSQPARPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSADASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTVEEVVVERNEKQAPNLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHPDHQAYPPYSPQAQPQPGHRIDSPGFKTASQQVYRKDPYPEEMMRQNHVLKQPAAVHPGQRPDKEQGLSYEPQPPYTERQAGRELEQPAYRYDASSYPEQFARNYDHRLRYDDRLLTYEEQWPYYDDKQPYQPRPLDGQPPAEPTERGYFPRFEEPVPLSYEGRAHYDPLPRTSTLRHEEPPAPAPAPGYDVHGRYRPDAQPYSTAGPKVPPEPKPYLDQYPRSYEPAPAQGFPSRAGPGHYEPLHSTTLAPPLGPSSQHKPEVPPTGSKPLPPPPAPAEEEEDPAMKPQSVLTRVKMFENKRSASMENRKDESHSTSFKPSEVTSKPAGAPVPGPKAPAPGQLGEHDRAAHRIPEPQKPHVKPPEDIVRANHYDPEEDEEYYRKQLSYFDRRSFEAKPPTHIPTSHLSEPTRPAHPQSQPQFAGYSSKGKAAEAEAVDRSFGEKRYDPLQATPPPPPLPAQYSQTAQPSPNTALGLHAHTKGAHGEGNAMSLDFQNSLVSKPDPPPSQNKPAAYRPAPREDAVQSAFYPQKSFPEKAPANGVEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPNETAHKPDLSSKAPASPKTLLKSAQPPEFDSGVETFSVHTDNKLKYQVNSVSTGPRAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006066398.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006066398.1

ORF Insert Sequence:

ATGGAACATG CTGCGTATTT TATCTCTCTT CGCCCCCGCC GCCCCATGGT AACGCCGTCC 
TCTGAAAACC CCAGTGGTGC TACTTCTAGT GTCAGCCAAG GAAAGCCTTC TTTAAGACGA 
ATTAAAGGGA GACTGCACAG GAGCAAGAGC CTCGATAGCA TCGATTTCTG TGAACTCACT 
AACACAACAA TGGAGGAAAC AGCTATATGG GAACAACACA CGGTGACACT TCACAGGGCT 
CCTGGATTTG GATTTGGAAT TGCAATATCT GGTGGAAGAG ATAATCCACA TTTCCAGAGC 
GGGGAAACCT CCATCGTGAT TTCAGATGTG CTAAAGGGGG GGCCTGCAGA AGGACAGCTG 
CAGGAAAATG ACCGGGTGGC AATGGTTAAT GGAGTCTCCA TGGATAATGT TGAGCATGCG 
TTTGCTGTTC AGCAACTAAG GAAAAGTGGG AAAAATGCAA AAATTACCAT TCGAAGGAAG 
AAGAAAGTTC AGATCCCAGT AAATCGTCCT GATCCTGAGC CTGTGTCTGA GAACGAAGAG 
GACAGTTACG ACGAGGAGGC GCATGACCCC AGAAGTGGCC GTGGGGCCCT GGCAAACAGG 
AGGAGCGAGA AGAGCTGGGG CCGAGATAGG AGTGCCAGTC GGGAGAGGAG TCTGTCCCCG 
AGGTCTGACA GGCGGTCTGT GGCCTCCAGT CAGCCTGCCA GACCCACCAA AGTCACCCTG 
GTGAAGTCCC GGAAGAATGA AGAATATGGT CTTCGATTGG CCAGCCATAT ATTTGTGAAG 
GAAATTTCAC AAGATAGTTT GGCAGCAAGA GATGGCAATA TTCAAGAAGG CGATGTTGTA 
TTGAAGATAA ATGGTACCGT GACGGAAAAC ATGTCACTGA CAGATGCGAA GACGCTGATC 
GAGAGGTCTA AGGGCAAGTT AAAGATGGTG GTTCAGAGAG ATGAGCGGGC TACCCTGCTG 
AATGTCCCTG ACCTTTCTGA CAGCATCCAC TCTGCTGACG CCTCCGAGAG GGACGATATT 
TCAGAAATCC AGTCACTGGC ATCAGACCAT TCGGGTCGAT CCCACGACCG GCCTCCCCGT 
CACAGCCGCT CTCGGTCTCC TGACCAGCGG TCCGAGCCTT CTGACCATTC TAGACACTCT 
CCACAGCAGC CCAGCAGTGG CAGTCTCCGG AGCAGAGAGG AAGAGAGAAT TTCTAAACCA 
GGGGCTGTCT CGACTCCGGT AAAGCATGCA GATGATCACA CACCTAAAAC AGTGGAAGAA 
GTTGTAGTTG AGCGAAATGA GAAACAAGCA CCCAATCTTC CAGAACCAAA GCCTGTGTAT 
GCCCAGGTAG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG TGTCCTGCCC 
AACTCAACTC ATGAAGATGG CATTCTTCGG CCCAGCATGA AGTTAGTAAA ATTCAGGAAA 
GGAGATAGTG TGGGTCTTCG GCTAGCTGGT GGAAACGATG TTGGCATATT TGTAGCTGGT 
GTTCTCGAAG ACAGCCCTGC TGCCAAAGAA GGCTTGGAGG AGGGCGACCA GATCCTCAGG 
GTAAACAATG TAGATTTCAC AAATATCATA AGAGAAGAAG CCGTCCTCTT CCTGCTTGAC 
CTCCCAAAAG GAGAAGAAGT GACCATATTG GCTCAGAAGA AAAAAGATGT TTATCGTCGC 
ATCGTAGAAT CCGATGTGGG TGATTCTTTC TATATCAGAA CCCATTTTGA ATATGAGAAG 
GAATCTCCCT ATGGACTCAG TTTTAACAAA GGAGAGGTGT TCCGTGTCGT GGACACTCTG 
TACAATGGGA AGCTGGGGTC CTGGCTCGCC ATTCGGATTG GCAAAAACCA TAAAGAGGTG 
GAACGTGGCA TCATCCCAAA CAAAAACAGA GCTGAGCAGT TAGCCAGTGT GCAGTATACA 
CTTCCCAAGA CAGCAGGGGG AGACCGCGCT GACTTCTGGA GGTTCCGCGG TCTGCGCAGC 
TCCAAGAGAA ACCTGCGCAA GAGCCGAGAA GACCTCTCAG CCCAGCCAGT CCAGACCAAG 
TTCCCGGCGT ATGAGAGGGT GGTTCTTCGA GAAGCTGGAT TCCTGAGGCC TGTAACCATC 
TTTGGACCAA TAGCTGATGT TGCCAGGGAG AAGCTGGCAA GAGAGGAGCC AGATATTTAC 
CAGATCGCAA AAAGTGAACC TCGGGATGCA GGAACCGACC AGCGCAGCTC TGGCATTATT 
CGCCTCCACA CAATAAAGCA GATAATAGAT CAGGACAAAC ATGCCTTGCT GGATGTAACG 
CCCAATGCAG TAGACCGTCT GAACTATGCC CAGTGGTATC CAATAGTGGT GTTCCTCAAC 
CCGGACTCAA AGCAGGGTGT GAAAACAATG CGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGCGCCAGGA AGTTATATGA GCGCTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGCTGGT ATGGTGCATT GAAAGAAGCA 
ATCCAGCAAC AACAGAACCA GTTGGTATGG GTTTCTGAGG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAGACCCTAA ATGATGAGGT GGGGACTCCC 
CCGGAGTCTG CCATTACCCG GTCCTCTGAG CCTGTCAGAG AGGACTCCTC TGGAATGCAT 
CCTGACCACC AGGCATATCC TCCCTACTCA CCACAAGCGC AGCCACAGCC AGGCCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGGTGT ACAGGAAGGA CCCCTACCCT 
GAGGAGATGA TGAGACAGAA CCACGTCTTG AAGCAGCCGG CTGCGGTTCA CCCTGGGCAG 
AGGCCAGACA AGGAGCAAGG CCTGAGCTAC GAGCCGCAGC CTCCGTACAC AGAGCGACAG 
GCTGGCAGGG AGTTGGAGCA GCCTGCATAC AGATATGATG CCTCTAGCTA CCCGGAGCAG 
TTTGCTCGGA ACTATGACCA TCGTCTGCGC TATGATGACC GCCTCCTCAC CTACGAGGAG 
CAGTGGCCCT ACTACGACGA CAAGCAGCCT TACCAGCCCC GGCCTCTTGA TGGTCAGCCT 
CCCGCGGAGC CAACAGAACG AGGGTATTTT CCCCGTTTTG AGGAGCCAGT GCCCCTGTCC 
TATGAAGGCC GAGCGCACTA TGACCCACTG CCCAGGACCT CCACCTTGCG GCACGAAGAG 
CCTCCTGCCC CTGCCCCTGC CCCTGGGTAC GACGTGCACG GCAGGTACCG GCCTGACGCG 
CAGCCCTACT CCACTGCTGG CCCCAAGGTG CCCCCTGAGC CCAAGCCGTA CTTGGACCAG 
TACCCTCGGA GTTACGAGCC AGCGCCAGCC CAGGGCTTCC CCTCCAGGGC AGGCCCAGGC 
CACTACGAGC CTCTTCACAG CACCACGCTC GCTCCACCAC TTGGCCCCTC GTCCCAGCAC 
AAGCCCGAAG TCCCGCCCAC AGGCTCCAAA CCCCTGCCTC CACCCCCAGC TCCAGCTGAG 
GAGGAGGAAG ACCCAGCCAT GAAGCCACAA TCCGTGCTCA CCAGAGTGAA GATGTTTGAG 
AACAAGAGGT CTGCTTCCAT GGAGAACAGG AAGGATGAGA GCCATTCCAC TAGTTTCAAG 
CCCTCAGAAG TCACGTCAAA ACCGGCAGGT GCTCCTGTCC CAGGTCCCAA AGCCCCTGCT 
CCGGGTCAGC TCGGTGAACA CGACAGAGCA GCGCATAGGA TTCCTGAACC ACAGAAGCCT 
CACGTGAAGC CACCTGAAGA CATTGTTCGG GCAAATCACT ATGACCCCGA GGAGGATGAG 
GAGTACTACC GGAAGCAGCT CTCCTACTTC GATCGGAGAA GCTTTGAGGC CAAGCCCCCC 
ACCCACATCC CCACTAGCCA CCTCTCCGAG CCCACCAGGC CGGCTCACCC CCAATCTCAG 
CCACAGTTTG CCGGTTACTC TTCCAAGGGG AAGGCTGCTG AGGCCGAAGC TGTGGACAGG 
TCATTCGGGG AGAAGCGCTA TGACCCGCTG CAGGCGACGC CCCCCCCTCC GCCGCTGCCC 
GCTCAGTACA GCCAGACTGC TCAACCCAGC CCCAACACGG CTCTCGGCCT CCACGCACAC 
ACCAAGGGGG CGCACGGTGA AGGTAATGCC ATGTCATTGG ATTTTCAGAA CTCGTTAGTG 
TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAG CTTACAGACC AGCCCCTCGA 
GAGGATGCTG TCCAGTCTGC CTTCTACCCA CAAAAGAGTT TCCCAGAGAA AGCCCCAGCT 
AATGGGGTCG AGCAGACGCA GAAAACCGTC ACGCCAGCAT ACAATCGATT CACACCAAAA 
CCATACACAA GCTCTGCACG GCCGTTTGAA CGCAAGTTTG AAAGCCCTAA ATTCAACCAC 
AATCTCTTGC CGAATGAAAC CGCACACAAA CCTGACCTGT CCTCCAAAGC CCCTGCTTCT 
CCGAAAACCC TCCTGAAGTC TGCACAGCCT CCTGAGTTTG ACAGTGGTGT GGAGACCTTC 
TCTGTCCACA CAGACAACAA GCTGAAATAC CAAGTAAACA GTGTTAGCAC GGGGCCGAGA 
GCAGTGCCCG TGAGTCCCTC AGCCGTGGAG GAAGATGAAG ATGAAGACGG TCACACAGTG 
GTGGCTACTG CCCGCGGCGT GTTTAACAGC AATGGTGGGG TGCTGAGCTC CATTGAGACC 
GGTGTCAGCA TCATCATCCC CCAGGGAGCC ATTCCTGAGG GGGTGGAACA GGAGATCTAC 
TTCAAGGTCT GCCGCGACAA CAGCATCCTG CCGCCTTTGG ACAAAGAGAA GGGTGAGACC 
CTGCTGAGCC CCCTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCCTAC CACACTGTGC GTCCATGACT CCTGACGGTT GGTCTTTTGC TCTAAAATCA 
TCCGACTCCT CGTCGGGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTATCTTG TTGGAGCAAA CTGTGTTTCT GTCCTGATTG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_006066399.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006066461.1 Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X7, mRNA.

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CloneID	OBu21761
Clone ID Related Accession (Same CDS sequence)	XM_006066399.1
Accession Version	XM_006066399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	tight junction protein ZO-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785206.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACATG CTGCGTATTT TATCTCTCTT CGCCCCCGCC GCCCCATGGT AACGCCGTCC 
TCTGAAAACC CCAGTGGTGC TACTTCTAGT GTCAGCCAAG GAAAGCCTTC TTTAAGACGA 
ATTAAAGGGA GACTGCACAG GAGCAAGAGC CTCGATAGCA TCGATTTCTG TGAACTCACT 
AACACAACAA TGGAGGAAAC AGCTATATGG GAACAACACA CGGTGACACT TCACAGGGCT 
CCTGGATTTG GATTTGGAAT TGCAATATCT GGTGGAAGAG ATAATCCACA TTTCCAGAGC 
GGGGAAACCT CCATCGTGAT TTCAGATGTG CTAAAGGGGG GGCCTGCAGA AGGACAGCTG 
CAGGAAAATG ACCGGGTGGC AATGGTTAAT GGAGTCTCCA TGGATAATGT TGAGCATGCG 
TTTGCTGTTC AGCAACTAAG GAAAAGTGGG AAAAATGCAA AAATTACCAT TCGAAGGAAG 
AAGAAAGTTC AGATCCCAGT AAATCGTCCT GATCCTGAGC CTGTGTCTGA GAACGAAGAG 
GACAGTTACG ACGAGGAGGC GCATGACCCC AGAAGTGGCC GTGGGGCCCT GGCAAACAGG 
AGGAGCGAGA AGAGCTGGGG CCGAGATAGG AGTGCCAGTC GGGAGAGGAG TCTGTCCCCG 
AGGTCTGACA GGCGGTCTGT GGCCTCCAGT CAGCCTGCCA GACCCACCAA AGTCACCCTG 
GTGAAGTCCC GGAAGAATGA AGAATATGGT CTTCGATTGG CCAGCCATAT ATTTGTGAAG 
GAAATTTCAC AAGATAGTTT GGCAGCAAGA GATGGCAATA TTCAAGAAGG CGATGTTGTA 
TTGAAGATAA ATGGTACCGT GACGGAAAAC ATGTCACTGA CAGATGCGAA GACGCTGATC 
GAGAGGTCTA AGGGCAAGTT AAAGATGGTG GTTCAGAGAG ATGAGCGGGC TACCCTGCTG 
AATGTCCCTG ACCTTTCTGA CAGCATCCAC TCTGCTGACG CCTCCGAGAG GGACGATATT 
TCAGAAATCC AGTCACTGGC ATCAGACCAT TCGGGTCGAT CCCACGACCG GCCTCCCCGT 
CACAGCCGCT CTCGGTCTCC TGACCAGCGG TCCGAGCCTT CTGACCATTC TAGACACTCT 
CCACAGCAGC CCAGCAGTGG CAGTCTCCGG AGCAGAGAGG AAGAGAGAAT TTCTAAACCA 
GGGGCTGTCT CGACTCCGGT AAAGCATGCA GATGATCACA CACCTAAAAC AGTGGAAGAA 
GTTGTAGTTG AGCGAAATGA GAAACAAGCA CCCAATCTTC CAGAACCAAA GCCTGTGTAT 
GCCCAGGTAG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG TGTCCTGCCC 
AACTCAACTC ATGAAGATGG CATTCTTCGG CCCAGCATGA AGTTAGTAAA ATTCAGGAAA 
GGAGATAGTG TGGGTCTTCG GCTAGCTGGT GGAAACGATG TTGGCATATT TGTAGCTGGT 
GTTCTCGAAG ACAGCCCTGC TGCCAAAGAA GGCTTGGAGG AGGGCGACCA GATCCTCAGG 
GTAAACAATG TAGATTTCAC AAATATCATA AGAGAAGAAG CCGTCCTCTT CCTGCTTGAC 
CTCCCAAAAG GAGAAGAAGT GACCATATTG GCTCAGAAGA AAAAAGATGT TTATCGTCGC 
ATCGTAGAAT CCGATGTGGG TGATTCTTTC TATATCAGAA CCCATTTTGA ATATGAGAAG 
GAATCTCCCT ATGGACTCAG TTTTAACAAA GGAGAGGTGT TCCGTGTCGT GGACACTCTG 
TACAATGGGA AGCTGGGGTC CTGGCTCGCC ATTCGGATTG GCAAAAACCA TAAAGAGGTG 
GAACGTGGCA TCATCCCAAA CAAAAACAGA GCTGAGCAGT TAGCCAGTGT GCAGTATACA 
CTTCCCAAGA CAGCAGGGGG AGACCGCGCT GACTTCTGGA GGTTCCGCGG TCTGCGCAGC 
TCCAAGAGAA ACCTGCGCAA GAGCCGAGAA GACCTCTCAG CCCAGCCAGT CCAGACCAAG 
TTCCCGGCGT ATGAGAGGGT GGTTCTTCGA GAAGCTGGAT TCCTGAGGCC TGTAACCATC 
TTTGGACCAA TAGCTGATGT TGCCAGGGAG AAGCTGGCAA GAGAGGAGCC AGATATTTAC 
CAGATCGCAA AAAGTGAACC TCGGGATGCA GGAACCGACC AGCGCAGCTC TGGCATTATT 
CGCCTCCACA CAATAAAGCA GATAATAGAT CAGGACAAAC ATGCCTTGCT GGATGTAACG 
CCCAATGCAG TAGACCGTCT GAACTATGCC CAGTGGTATC CAATAGTGGT GTTCCTCAAC 
CCGGACTCAA AGCAGGGTGT GAAAACAATG CGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGCGCCAGGA AGTTATATGA GCGCTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGCTGGT ATGGTGCATT GAAAGAAGCA 
ATCCAGCAAC AACAGAACCA GTTGGTATGG GTTTCTGAGG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAGACCCTAA ATGATGAGGT GGGGACTCCC 
CCGGAGTCTG CCATTACCCG GTCCTCTGAG CCTGTCAGAG AGGACTCCTC TGGAATGCAT 
CCTGACCACC AGGCATATCC TCCCTACTCA CCACAAGCGC AGCCACAGCC AGGCCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGGTGT ACAGGAAGGA CCCCTACCCT 
GAGGAGATGA TGAGACAGAA CCACGTCTTG AAGCAGCCGG CTGCGGTTCA CCCTGGGCAG 
AGGCCAGACA AGGAGCAAGG CCTGAGCTAC GAGCCGCAGC CTCCGTACAC AGAGCGACAG 
GCTGGCAGGG AGTTGGAGCA GCCTGCATAC AGATATGATG CCTCTAGCTA CCCGGAGCAG 
TTTGCTCGGA ACTATGACCA TCGTCTGCGC TATGATGACC GCCTCCTCAC CTACGAGGAG 
CAGTGGCCCT ACTACGACGA CAAGCAGCCT TACCAGCCCC GGCCTCTTGA TGGTCAGCCT 
CCCGCGGAGC CAACAGAACG AGGGTATTTT CCCCGTTTTG AGGAGCCAGT GCCCCTGTCC 
TATGAAGGCC GAGCGCACTA TGACCCACTG CCCAGGACCT CCACCTTGCG GCACGAAGAG 
CCTCCTGCCC CTGCCCCTGC CCCTGGGTAC GACGTGCACG GCAGGTACCG GCCTGACGCG 
CAGCCCTACT CCACTGCTGG CCCCAAGGTG CCCCCTGAGC CCAAGCCGTA CTTGGACCAG 
TACCCTCGGA GTTACGAGCC AGCGCCAGCC CAGGGCTTCC CCTCCAGGGC AGGCCCAGGC 
CACTACGAGC CTCTTCACAG CACCACGCTC GCTCCACCAC TTGGCCCCTC GTCCCAGCAC 
AAGCCCGAAG TCCCGCCCAC AGGCTCCAAA CCCCTGCCTC CACCCCCAGC TCCAGCTGAG 
GAGGAGGAAG ACCCAGCCAT GAAGCCACAA TCCGTGCTCA CCAGAGTGAA GATGTTTGAG 
AACAAGAGGT CTGCTTCCAT GGAGAACAGG AAGGATGAGA GCCATTCCAC TAGTTTCAAG 
CCCTCAGAAG TCACGTCAAA ACCGGCAGGT GCTCCTGTCC CAGGTCCCAA AGCCCCTGCT 
CCGGGTCAGC TCGGTGAACA CGACAGAGCA GCGCATAGGA TTCCTGAACC ACAGAAGCCT 
CACGTGAAGC CACCTGAAGA CATTGTTCGG GCAAATCACT ATGACCCCGA GGAGGATGAG 
GAGTACTACC GGAAGCAGCT CTCCTACTTC GATCGGAGAA GCTTTGAGGC CAAGCCCCCC 
ACCCACATCC CCACTAGCCA CCTCTCCGAG CCCACCAGGC CGGCTCACCC CCAATCTCAG 
CCACAGTTTG CCGGTTACTC TTCCAAGGGG AAGGCTGCTG AGGCCGAAGC TGTGGACAGG 
TCATTCGGGG AGAAGCGCTA TGACCCGCTG CAGGCGACGC CCCCCCCTCC GCCGCTGCCC 
GCTCAGTACA GCCAGACTGC TCAACCCAGC CCCAACACGG CTCTCGGCCT CCACGCACAC 
ACCAAGGGGG CGCACGGTGA AGGTAATGCC ATGTCATTGG ATTTTCAGAA CTCGTTAGTG 
TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAG CTTACAGACC AGCCCCTCGA 
GAGGATGCTG TCCAGTCTGC CTTCTACCCA CAAAAGAGTT TCCCAGAGAA AGCCCCAGCT 
AATGGGGTCG AGCAGACGCA GAAAACCGTC ACGCCAGCAT ACAATCGATT CACACCAAAA 
CCATACACAA GCTCTGCACG GCCGTTTGAA CGCAAGTTTG AAAGCCCTAA ATTCAACCAC 
AATCTCTTGC CGAATGAAAC CGCACACAAA CCTGACCTGT CCTCCAAAGC CCCTGCTTCT 
CCGAAAACCC TCCTGAAGTC TGCACAGCCT CCTGAGTTTG ACAGTGGTGT GGAGACCTTC 
TCTGTCCACA CAGACAACAA GCTGAAATAC CAAGTAAACA GTGTTAGCAC GGGGCCGAGA 
GCAGTGCCCG TGAGTCCCTC AGCCGTGGAG GAAGATGAAG ATGAAGACGG TCACACAGTG 
GTGGCTACTG CCCGCGGCGT GTTTAACAGC AATGGTGGGG TGCTGAGCTC CATTGAGACC 
GGTGTCAGCA TCATCATCCC CCAGGGAGCC ATTCCTGAGG GGGTGGAACA GGAGATCTAC 
TTCAAGGTCT GCCGCGACAA CAGCATCCTG CCGCCTTTGG ACAAAGAGAA GGGTGAGACC 
CTGCTGAGCC CCCTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCCTAC CACACTGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTATCTTG TTGGAGCAAA CTGTGTTTCT GTCCTGATTG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006066461.1
CDS	1..5151
Translation	MEHAAYFISLRPRRPMVTPSSENPSGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTTMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVNRPDPEPVSENEEDSYDEEAHDPRSGRGALANRRSEKSWGRDRSASRERSLSPRSDRRSVASSQPARPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSADASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTVEEVVVERNEKQAPNLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHPDHQAYPPYSPQAQPQPGHRIDSPGFKTASQQVYRKDPYPEEMMRQNHVLKQPAAVHPGQRPDKEQGLSYEPQPPYTERQAGRELEQPAYRYDASSYPEQFARNYDHRLRYDDRLLTYEEQWPYYDDKQPYQPRPLDGQPPAEPTERGYFPRFEEPVPLSYEGRAHYDPLPRTSTLRHEEPPAPAPAPGYDVHGRYRPDAQPYSTAGPKVPPEPKPYLDQYPRSYEPAPAQGFPSRAGPGHYEPLHSTTLAPPLGPSSQHKPEVPPTGSKPLPPPPAPAEEEEDPAMKPQSVLTRVKMFENKRSASMENRKDESHSTSFKPSEVTSKPAGAPVPGPKAPAPGQLGEHDRAAHRIPEPQKPHVKPPEDIVRANHYDPEEDEEYYRKQLSYFDRRSFEAKPPTHIPTSHLSEPTRPAHPQSQPQFAGYSSKGKAAEAEAVDRSFGEKRYDPLQATPPPPPLPAQYSQTAQPSPNTALGLHAHTKGAHGEGNAMSLDFQNSLVSKPDPPPSQNKPAAYRPAPREDAVQSAFYPQKSFPEKAPANGVEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPNETAHKPDLSSKAPASPKTLLKSAQPPEFDSGVETFSVHTDNKLKYQVNSVSTGPRAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_006066399.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006066399.1

ORF Insert Sequence:

ATGGAACATG CTGCGTATTT TATCTCTCTT CGCCCCCGCC GCCCCATGGT AACGCCGTCC 
TCTGAAAACC CCAGTGGTGC TACTTCTAGT GTCAGCCAAG GAAAGCCTTC TTTAAGACGA 
ATTAAAGGGA GACTGCACAG GAGCAAGAGC CTCGATAGCA TCGATTTCTG TGAACTCACT 
AACACAACAA TGGAGGAAAC AGCTATATGG GAACAACACA CGGTGACACT TCACAGGGCT 
CCTGGATTTG GATTTGGAAT TGCAATATCT GGTGGAAGAG ATAATCCACA TTTCCAGAGC 
GGGGAAACCT CCATCGTGAT TTCAGATGTG CTAAAGGGGG GGCCTGCAGA AGGACAGCTG 
CAGGAAAATG ACCGGGTGGC AATGGTTAAT GGAGTCTCCA TGGATAATGT TGAGCATGCG 
TTTGCTGTTC AGCAACTAAG GAAAAGTGGG AAAAATGCAA AAATTACCAT TCGAAGGAAG 
AAGAAAGTTC AGATCCCAGT AAATCGTCCT GATCCTGAGC CTGTGTCTGA GAACGAAGAG 
GACAGTTACG ACGAGGAGGC GCATGACCCC AGAAGTGGCC GTGGGGCCCT GGCAAACAGG 
AGGAGCGAGA AGAGCTGGGG CCGAGATAGG AGTGCCAGTC GGGAGAGGAG TCTGTCCCCG 
AGGTCTGACA GGCGGTCTGT GGCCTCCAGT CAGCCTGCCA GACCCACCAA AGTCACCCTG 
GTGAAGTCCC GGAAGAATGA AGAATATGGT CTTCGATTGG CCAGCCATAT ATTTGTGAAG 
GAAATTTCAC AAGATAGTTT GGCAGCAAGA GATGGCAATA TTCAAGAAGG CGATGTTGTA 
TTGAAGATAA ATGGTACCGT GACGGAAAAC ATGTCACTGA CAGATGCGAA GACGCTGATC 
GAGAGGTCTA AGGGCAAGTT AAAGATGGTG GTTCAGAGAG ATGAGCGGGC TACCCTGCTG 
AATGTCCCTG ACCTTTCTGA CAGCATCCAC TCTGCTGACG CCTCCGAGAG GGACGATATT 
TCAGAAATCC AGTCACTGGC ATCAGACCAT TCGGGTCGAT CCCACGACCG GCCTCCCCGT 
CACAGCCGCT CTCGGTCTCC TGACCAGCGG TCCGAGCCTT CTGACCATTC TAGACACTCT 
CCACAGCAGC CCAGCAGTGG CAGTCTCCGG AGCAGAGAGG AAGAGAGAAT TTCTAAACCA 
GGGGCTGTCT CGACTCCGGT AAAGCATGCA GATGATCACA CACCTAAAAC AGTGGAAGAA 
GTTGTAGTTG AGCGAAATGA GAAACAAGCA CCCAATCTTC CAGAACCAAA GCCTGTGTAT 
GCCCAGGTAG GACAGCCAGA CGTGGATTTA CCTGTCAGTC CATCTGATGG TGTCCTGCCC 
AACTCAACTC ATGAAGATGG CATTCTTCGG CCCAGCATGA AGTTAGTAAA ATTCAGGAAA 
GGAGATAGTG TGGGTCTTCG GCTAGCTGGT GGAAACGATG TTGGCATATT TGTAGCTGGT 
GTTCTCGAAG ACAGCCCTGC TGCCAAAGAA GGCTTGGAGG AGGGCGACCA GATCCTCAGG 
GTAAACAATG TAGATTTCAC AAATATCATA AGAGAAGAAG CCGTCCTCTT CCTGCTTGAC 
CTCCCAAAAG GAGAAGAAGT GACCATATTG GCTCAGAAGA AAAAAGATGT TTATCGTCGC 
ATCGTAGAAT CCGATGTGGG TGATTCTTTC TATATCAGAA CCCATTTTGA ATATGAGAAG 
GAATCTCCCT ATGGACTCAG TTTTAACAAA GGAGAGGTGT TCCGTGTCGT GGACACTCTG 
TACAATGGGA AGCTGGGGTC CTGGCTCGCC ATTCGGATTG GCAAAAACCA TAAAGAGGTG 
GAACGTGGCA TCATCCCAAA CAAAAACAGA GCTGAGCAGT TAGCCAGTGT GCAGTATACA 
CTTCCCAAGA CAGCAGGGGG AGACCGCGCT GACTTCTGGA GGTTCCGCGG TCTGCGCAGC 
TCCAAGAGAA ACCTGCGCAA GAGCCGAGAA GACCTCTCAG CCCAGCCAGT CCAGACCAAG 
TTCCCGGCGT ATGAGAGGGT GGTTCTTCGA GAAGCTGGAT TCCTGAGGCC TGTAACCATC 
TTTGGACCAA TAGCTGATGT TGCCAGGGAG AAGCTGGCAA GAGAGGAGCC AGATATTTAC 
CAGATCGCAA AAAGTGAACC TCGGGATGCA GGAACCGACC AGCGCAGCTC TGGCATTATT 
CGCCTCCACA CAATAAAGCA GATAATAGAT CAGGACAAAC ATGCCTTGCT GGATGTAACG 
CCCAATGCAG TAGACCGTCT GAACTATGCC CAGTGGTATC CAATAGTGGT GTTCCTCAAC 
CCGGACTCAA AGCAGGGTGT GAAAACAATG CGGATGAGGT TATGTCCAGA ATCTCGGAAA 
AGCGCCAGGA AGTTATATGA GCGCTCTCAT AAACTTCGTA AAAATAATCA CCATCTTTTT 
ACAACTACAA TTAACTTAAA TTCAATGAAT GATGGCTGGT ATGGTGCATT GAAAGAAGCA 
ATCCAGCAAC AACAGAACCA GTTGGTATGG GTTTCTGAGG GAAAGGCGGA TGGTGCTACA 
AGTGATGACC TTGATTTGCA TGATGATCGT CTGTCCTACC TGTCAGCCCC AGGTAGTGAA 
TACTCAATGT ATAGCACGGA CAGTAGACAC ACTTCTGACT ATGAAGACAC AGATACAGAA 
GGCGGGGCCT ACACTGATCA AGAACTAGAT GAGACCCTAA ATGATGAGGT GGGGACTCCC 
CCGGAGTCTG CCATTACCCG GTCCTCTGAG CCTGTCAGAG AGGACTCCTC TGGAATGCAT 
CCTGACCACC AGGCATATCC TCCCTACTCA CCACAAGCGC AGCCACAGCC AGGCCATAGA 
ATAGACTCCC CTGGATTTAA AACAGCCTCT CAACAGGTGT ACAGGAAGGA CCCCTACCCT 
GAGGAGATGA TGAGACAGAA CCACGTCTTG AAGCAGCCGG CTGCGGTTCA CCCTGGGCAG 
AGGCCAGACA AGGAGCAAGG CCTGAGCTAC GAGCCGCAGC CTCCGTACAC AGAGCGACAG 
GCTGGCAGGG AGTTGGAGCA GCCTGCATAC AGATATGATG CCTCTAGCTA CCCGGAGCAG 
TTTGCTCGGA ACTATGACCA TCGTCTGCGC TATGATGACC GCCTCCTCAC CTACGAGGAG 
CAGTGGCCCT ACTACGACGA CAAGCAGCCT TACCAGCCCC GGCCTCTTGA TGGTCAGCCT 
CCCGCGGAGC CAACAGAACG AGGGTATTTT CCCCGTTTTG AGGAGCCAGT GCCCCTGTCC 
TATGAAGGCC GAGCGCACTA TGACCCACTG CCCAGGACCT CCACCTTGCG GCACGAAGAG 
CCTCCTGCCC CTGCCCCTGC CCCTGGGTAC GACGTGCACG GCAGGTACCG GCCTGACGCG 
CAGCCCTACT CCACTGCTGG CCCCAAGGTG CCCCCTGAGC CCAAGCCGTA CTTGGACCAG 
TACCCTCGGA GTTACGAGCC AGCGCCAGCC CAGGGCTTCC CCTCCAGGGC AGGCCCAGGC 
CACTACGAGC CTCTTCACAG CACCACGCTC GCTCCACCAC TTGGCCCCTC GTCCCAGCAC 
AAGCCCGAAG TCCCGCCCAC AGGCTCCAAA CCCCTGCCTC CACCCCCAGC TCCAGCTGAG 
GAGGAGGAAG ACCCAGCCAT GAAGCCACAA TCCGTGCTCA CCAGAGTGAA GATGTTTGAG 
AACAAGAGGT CTGCTTCCAT GGAGAACAGG AAGGATGAGA GCCATTCCAC TAGTTTCAAG 
CCCTCAGAAG TCACGTCAAA ACCGGCAGGT GCTCCTGTCC CAGGTCCCAA AGCCCCTGCT 
CCGGGTCAGC TCGGTGAACA CGACAGAGCA GCGCATAGGA TTCCTGAACC ACAGAAGCCT 
CACGTGAAGC CACCTGAAGA CATTGTTCGG GCAAATCACT ATGACCCCGA GGAGGATGAG 
GAGTACTACC GGAAGCAGCT CTCCTACTTC GATCGGAGAA GCTTTGAGGC CAAGCCCCCC 
ACCCACATCC CCACTAGCCA CCTCTCCGAG CCCACCAGGC CGGCTCACCC CCAATCTCAG 
CCACAGTTTG CCGGTTACTC TTCCAAGGGG AAGGCTGCTG AGGCCGAAGC TGTGGACAGG 
TCATTCGGGG AGAAGCGCTA TGACCCGCTG CAGGCGACGC CCCCCCCTCC GCCGCTGCCC 
GCTCAGTACA GCCAGACTGC TCAACCCAGC CCCAACACGG CTCTCGGCCT CCACGCACAC 
ACCAAGGGGG CGCACGGTGA AGGTAATGCC ATGTCATTGG ATTTTCAGAA CTCGTTAGTG 
TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAG CTTACAGACC AGCCCCTCGA 
GAGGATGCTG TCCAGTCTGC CTTCTACCCA CAAAAGAGTT TCCCAGAGAA AGCCCCAGCT 
AATGGGGTCG AGCAGACGCA GAAAACCGTC ACGCCAGCAT ACAATCGATT CACACCAAAA 
CCATACACAA GCTCTGCACG GCCGTTTGAA CGCAAGTTTG AAAGCCCTAA ATTCAACCAC 
AATCTCTTGC CGAATGAAAC CGCACACAAA CCTGACCTGT CCTCCAAAGC CCCTGCTTCT 
CCGAAAACCC TCCTGAAGTC TGCACAGCCT CCTGAGTTTG ACAGTGGTGT GGAGACCTTC 
TCTGTCCACA CAGACAACAA GCTGAAATAC CAAGTAAACA GTGTTAGCAC GGGGCCGAGA 
GCAGTGCCCG TGAGTCCCTC AGCCGTGGAG GAAGATGAAG ATGAAGACGG TCACACAGTG 
GTGGCTACTG CCCGCGGCGT GTTTAACAGC AATGGTGGGG TGCTGAGCTC CATTGAGACC 
GGTGTCAGCA TCATCATCCC CCAGGGAGCC ATTCCTGAGG GGGTGGAACA GGAGATCTAC 
TTCAAGGTCT GCCGCGACAA CAGCATCCTG CCGCCTTTGG ACAAAGAGAA GGGTGAGACC 
CTGCTGAGCC CCCTAGTGAT GTGTGGGCCC CATGGCCTCA AGTTCCTGAA GCCCGTGGAG 
CTGCGCCTAC CACACTGTGA TCCTAAAACC TGGCAAAACA AGTGTCTTCC TGGAGATCCA 
AATTATCTTG TTGGAGCAAA CTGTGTTTCT GTCCTGATTG ACCACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_025271181.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025126966.1 Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X1, mRNA.

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CloneID	OBu56738
Clone ID Related Accession (Same CDS sequence)	XM_025271181.1
Accession Version	XM_025271181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	tight junction protein ZO-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC 
GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG 
TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA 
CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC 
ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG 
AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTAAAGG GGGGGCCTGC AGAAGGACAG 
CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT 
GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATTAC CATTCGAAGG 
AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA 
GAGGACAGTT ACGACGAGGA GGCGCATGAC CCCAGAAGTG GCCGTGGGGC CCTGGCAAAC 
AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC 
CCGAGGTCTG ACAGGCGGTC TGTGGCCTCC AGTCAGCCTG CCAGACCCAC CAAAGTCACC 
CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG 
ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG 
CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT 
ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCGGGTC GATCCCACGA CCGGCCTCCC 
CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ACACACCTAA AACAGTGGAA 
GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG 
TATGCCCAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGTGTCCTG 
CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG 
AAAGGAGATA GTGTGGGTCT TCGGCTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT 
GGTGTTCTCG AAGACAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC 
AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT 
GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAAAAAGA TGTTTATCGT 
CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG 
AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT 
CTGTACAATG GGAAGCTGGG GTCCTGGCTC GCCATTCGGA TTGGCAAAAA CCATAAAGAG 
GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT 
ACACTTCCCA AGACAGCAGG GGGAGACCGC GCTGACTTCT GGAGGTTCCG CGGTCTGCGC 
AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC 
AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC 
ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAGCTGG CAAGAGAGGA GCCAGATATT 
TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT 
ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT GCTGGATGTA 
ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATAGT GGTGTTCCTC 
AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGGATGA GGTTATGTCC AGAATCTCGG 
AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT 
TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ATTGAAAGAA 
GCAATCCAGC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT 
ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT 
GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGATACA 
GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT 
CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG 
CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT 
AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGA AAGCAGAAGC CTCATCTCCA 
GGCCCTTACC TTTCGCCTGA AACAAACCCG GCACCATCAG CCTCTGCTGT TAATCCTCAC 
GTAGACTTAA CTAATGTCAG ACTGGAGGAG CCGAGCCCAG CTCCCCCTTC CTCTGACTCA 
CCACACGCCG ACTCTTTACG AGCTCCAGGC ACTGAGGCCG CTCACGTGCA GCTCAGAGGT 
CCAGAGCCGT CCCTGTCGTC GCATGTGGGC CCAGCAAAGG TGTACAGGAA GGACCCCTAC 
CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGCGGT TCACCCTGGG 
CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA 
CAGGCTGGCA GGGAGTTGGA GCAGCCTGCA TACAGATATG ATGCCTCTAG CTACCCGGAG 
CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGATG ACCGCCTCCT CACCTACGAG 
GAGCAGTGGC CCTACTACGA CGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG 
CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTTCCCCGTT TTGAGGAGCC AGTGCCCCTG 
TCCTATGAAG GCCGAGCGCA CTATGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA 
GAGCCTCCTG CCCCTGCCCC TGCCCCTGGG TACGACGTGC ACGGCAGGTA CCGGCCTGAC 
GCGCAGCCCT ACTCCACTGC TGGCCCCAAG GTGCCCCCTG AGCCCAAGCC GTACTTGGAC 
CAGTACCCTC GGAGTTACGA GCCAGCGCCA GCCCAGGGCT TCCCCTCCAG GGCAGGCCCA 
GGCCACTACG AGCCTCTTCA CAGCACCACG CTCGCTCCAC CACTTGGCCC CTCGTCCCAG 
CACAAGCCCG AAGTCCCGCC CACAGGCTCC AAACCCCTGC CTCCACCCCC AGCTCCAGCT 
GAGGAGGAGG AAGACCCAGC CATGAAGCCA CAATCCGTGC TCACCAGAGT GAAGATGTTT 
GAGAACAAGA GGTCTGCTTC CATGGAGAAC AGGAAGGATG AGAGCCATTC CACTAGTTTC 
AAGCCCTCAG AAGTCACGTC AAAACCGGCA GGTGCTCCTG TCCCAGGTCC CAAAGCCCCT 
GCTCCGGGTC AGCTCGGTGA ACACGACAGA GCAGCGCATA GGATTCCTGA ACCACAGAAG 
CCTCACGTGA AGCCACCTGA AGACATTGTT CGGGCAAATC ACTATGACCC CGAGGAGGAT 
GAGGAGTACT ACCGGAAGCA GCTCTCCTAC TTCGATCGGA GAAGCTTTGA GGCCAAGCCC 
CCCACCCACA TCCCCACTAG CCACCTCTCC GAGCCCACCA GGCCGGCTCA CCCCCAATCT 
CAGCCACAGT TTGCCGGTTA CTCTTCCAAG GGGAAGGCTG CTGAGGCCGA AGCTGTGGAC 
AGGTCATTCG GGGAGAAGCG CTATGACCCG CTGCAGGCGA CGCCCCCCCC TCCGCCGCTG 
CCCGCTCAGT ACAGCCAGAC TGCTCAACCC AGCCCCAACA CGGCTCTCGG CCTCCACGCA 
CACACCAAGG GGGCGCACGG TGAAGGTAAT GCCATGTCAT TGGATTTTCA GAACTCGTTA 
GTGTCCAAAC CAGACCCACC TCCATCTCAG AATAAGCCAG CAGCTTACAG ACCAGCCCCT 
CGAGAGGATG CTGTCCAGTC TGCCTTCTAC CCACAAAAGA GTTTCCCAGA GAAAGCCCCA 
GCTAATGGGG TCGAGCAGAC GCAGAAAACC GTCACGCCAG CATACAATCG ATTCACACCA 
AAACCATACA CAAGCTCTGC ACGGCCGTTT GAACGCAAGT TTGAAAGCCC TAAATTCAAC 
CACAATCTCT TGCCGAATGA AACCGCACAC AAACCTGACC TGTCCTCCAA AGCCCCTGCT 
TCTCCGAAAA CCCTCCTGAA GTCTGCACAG CCTCCTGAGT TTGACAGTGG TGTGGAGACC 
TTCTCTGTCC ACACAGACAA CAAGCTGAAA TACCAAGTAA ACAGTGTTAG CACGGGGCCG 
AGAGCAGTGC CCGTGAGTCC CTCAGCCGTG GAGGAAGATG AAGATGAAGA CGGTCACACA 
GTGGTGGCTA CTGCCCGCGG CGTGTTTAAC AGCAATGGTG GGGTGCTGAG CTCCATTGAG 
ACCGGTGTCA GCATCATCAT CCCCCAGGGA GCCATTCCTG AGGGGGTGGA ACAGGAGATC 
TACTTCAAGG TCTGCCGCGA CAACAGCATC CTGCCGCCTT TGGACAAAGA GAAGGGTGAG 
ACCCTGCTGA GCCCCCTAGT GATGTGTGGG CCCCATGGCC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCC TACCACACTG TGCGTCCATG ACTCCTGACG GTTGGTCTTT TGCTCTAAAA 
TCATCCGACT CCTCGTCGGG TGATCCTAAA ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT 
CCAAATTATC TTGTTGGAGC AAACTGTGTT TCTGTCCTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025126966.1
CDS	37..5610
Translation	MRRLEGLCLRRRRRRGPGRERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRRLWVTPSSENPSGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTTMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVNRPDPEPVSENEEDSYDEEAHDPRSGRGALANRRSEKSWGRDRSASRERSLSPRSDRRSVASSQPARPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSADASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTVEEVVVERNEKQAPNLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHPDHQAYPPYSPQAQPQPGHRIDSPGFKTASQQKAEASSPGPYLSPETNPAPSASAVNPHVDLTNVRLEEPSPAPPSSDSPHADSLRAPGTEAAHVQLRGPEPSLSSHVGPAKVYRKDPYPEEMMRQNHVLKQPAAVHPGQRPDKEQGLSYEPQPPYTERQAGRELEQPAYRYDASSYPEQFARNYDHRLRYDDRLLTYEEQWPYYDDKQPYQPRPLDGQPPAEPTERGYFPRFEEPVPLSYEGRAHYDPLPRTSTLRHEEPPAPAPAPGYDVHGRYRPDAQPYSTAGPKVPPEPKPYLDQYPRSYEPAPAQGFPSRAGPGHYEPLHSTTLAPPLGPSSQHKPEVPPTGSKPLPPPPAPAEEEEDPAMKPQSVLTRVKMFENKRSASMENRKDESHSTSFKPSEVTSKPAGAPVPGPKAPAPGQLGEHDRAAHRIPEPQKPHVKPPEDIVRANHYDPEEDEEYYRKQLSYFDRRSFEAKPPTHIPTSHLSEPTRPAHPQSQPQFAGYSSKGKAAEAEAVDRSFGEKRYDPLQATPPPPPLPAQYSQTAQPSPNTALGLHAHTKGAHGEGNAMSLDFQNSLVSKPDPPPSQNKPAAYRPAPREDAVQSAFYPQKSFPEKAPANGVEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPNETAHKPDLSSKAPASPKTLLKSAQPPEFDSGVETFSVHTDNKLKYQVNSVSTGPRAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_025271181.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025271181.1

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC 
GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG 
TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA 
CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC 
ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG 
AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTAAAGG GGGGGCCTGC AGAAGGACAG 
CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT 
GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATTAC CATTCGAAGG 
AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA 
GAGGACAGTT ACGACGAGGA GGCGCATGAC CCCAGAAGTG GCCGTGGGGC CCTGGCAAAC 
AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC 
CCGAGGTCTG ACAGGCGGTC TGTGGCCTCC AGTCAGCCTG CCAGACCCAC CAAAGTCACC 
CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG 
ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG 
CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT 
ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCGGGTC GATCCCACGA CCGGCCTCCC 
CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ACACACCTAA AACAGTGGAA 
GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG 
TATGCCCAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGTGTCCTG 
CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG 
AAAGGAGATA GTGTGGGTCT TCGGCTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT 
GGTGTTCTCG AAGACAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC 
AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT 
GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAAAAAGA TGTTTATCGT 
CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG 
AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT 
CTGTACAATG GGAAGCTGGG GTCCTGGCTC GCCATTCGGA TTGGCAAAAA CCATAAAGAG 
GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT 
ACACTTCCCA AGACAGCAGG GGGAGACCGC GCTGACTTCT GGAGGTTCCG CGGTCTGCGC 
AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC 
AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC 
ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAGCTGG CAAGAGAGGA GCCAGATATT 
TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT 
ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT GCTGGATGTA 
ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATAGT GGTGTTCCTC 
AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGGATGA GGTTATGTCC AGAATCTCGG 
AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT 
TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ATTGAAAGAA 
GCAATCCAGC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT 
ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT 
GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGATACA 
GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT 
CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG 
CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT 
AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGA AAGCAGAAGC CTCATCTCCA 
GGCCCTTACC TTTCGCCTGA AACAAACCCG GCACCATCAG CCTCTGCTGT TAATCCTCAC 
GTAGACTTAA CTAATGTCAG ACTGGAGGAG CCGAGCCCAG CTCCCCCTTC CTCTGACTCA 
CCACACGCCG ACTCTTTACG AGCTCCAGGC ACTGAGGCCG CTCACGTGCA GCTCAGAGGT 
CCAGAGCCGT CCCTGTCGTC GCATGTGGGC CCAGCAAAGG TGTACAGGAA GGACCCCTAC 
CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGCGGT TCACCCTGGG 
CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA 
CAGGCTGGCA GGGAGTTGGA GCAGCCTGCA TACAGATATG ATGCCTCTAG CTACCCGGAG 
CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGATG ACCGCCTCCT CACCTACGAG 
GAGCAGTGGC CCTACTACGA CGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG 
CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTTCCCCGTT TTGAGGAGCC AGTGCCCCTG 
TCCTATGAAG GCCGAGCGCA CTATGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA 
GAGCCTCCTG CCCCTGCCCC TGCCCCTGGG TACGACGTGC ACGGCAGGTA CCGGCCTGAC 
GCGCAGCCCT ACTCCACTGC TGGCCCCAAG GTGCCCCCTG AGCCCAAGCC GTACTTGGAC 
CAGTACCCTC GGAGTTACGA GCCAGCGCCA GCCCAGGGCT TCCCCTCCAG GGCAGGCCCA 
GGCCACTACG AGCCTCTTCA CAGCACCACG CTCGCTCCAC CACTTGGCCC CTCGTCCCAG 
CACAAGCCCG AAGTCCCGCC CACAGGCTCC AAACCCCTGC CTCCACCCCC AGCTCCAGCT 
GAGGAGGAGG AAGACCCAGC CATGAAGCCA CAATCCGTGC TCACCAGAGT GAAGATGTTT 
GAGAACAAGA GGTCTGCTTC CATGGAGAAC AGGAAGGATG AGAGCCATTC CACTAGTTTC 
AAGCCCTCAG AAGTCACGTC AAAACCGGCA GGTGCTCCTG TCCCAGGTCC CAAAGCCCCT 
GCTCCGGGTC AGCTCGGTGA ACACGACAGA GCAGCGCATA GGATTCCTGA ACCACAGAAG 
CCTCACGTGA AGCCACCTGA AGACATTGTT CGGGCAAATC ACTATGACCC CGAGGAGGAT 
GAGGAGTACT ACCGGAAGCA GCTCTCCTAC TTCGATCGGA GAAGCTTTGA GGCCAAGCCC 
CCCACCCACA TCCCCACTAG CCACCTCTCC GAGCCCACCA GGCCGGCTCA CCCCCAATCT 
CAGCCACAGT TTGCCGGTTA CTCTTCCAAG GGGAAGGCTG CTGAGGCCGA AGCTGTGGAC 
AGGTCATTCG GGGAGAAGCG CTATGACCCG CTGCAGGCGA CGCCCCCCCC TCCGCCGCTG 
CCCGCTCAGT ACAGCCAGAC TGCTCAACCC AGCCCCAACA CGGCTCTCGG CCTCCACGCA 
CACACCAAGG GGGCGCACGG TGAAGGTAAT GCCATGTCAT TGGATTTTCA GAACTCGTTA 
GTGTCCAAAC CAGACCCACC TCCATCTCAG AATAAGCCAG CAGCTTACAG ACCAGCCCCT 
CGAGAGGATG CTGTCCAGTC TGCCTTCTAC CCACAAAAGA GTTTCCCAGA GAAAGCCCCA 
GCTAATGGGG TCGAGCAGAC GCAGAAAACC GTCACGCCAG CATACAATCG ATTCACACCA 
AAACCATACA CAAGCTCTGC ACGGCCGTTT GAACGCAAGT TTGAAAGCCC TAAATTCAAC 
CACAATCTCT TGCCGAATGA AACCGCACAC AAACCTGACC TGTCCTCCAA AGCCCCTGCT 
TCTCCGAAAA CCCTCCTGAA GTCTGCACAG CCTCCTGAGT TTGACAGTGG TGTGGAGACC 
TTCTCTGTCC ACACAGACAA CAAGCTGAAA TACCAAGTAA ACAGTGTTAG CACGGGGCCG 
AGAGCAGTGC CCGTGAGTCC CTCAGCCGTG GAGGAAGATG AAGATGAAGA CGGTCACACA 
GTGGTGGCTA CTGCCCGCGG CGTGTTTAAC AGCAATGGTG GGGTGCTGAG CTCCATTGAG 
ACCGGTGTCA GCATCATCAT CCCCCAGGGA GCCATTCCTG AGGGGGTGGA ACAGGAGATC 
TACTTCAAGG TCTGCCGCGA CAACAGCATC CTGCCGCCTT TGGACAAAGA GAAGGGTGAG 
ACCCTGCTGA GCCCCCTAGT GATGTGTGGG CCCCATGGCC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCC TACCACACTG TGCGTCCATG ACTCCTGACG GTTGGTCTTT TGCTCTAAAA 
TCATCCGACT CCTCGTCGGG TGATCCTAAA ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT 
CCAAATTATC TTGTTGGAGC AAACTGTGTT TCTGTCCTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_025271182.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025126967.1 Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X2, mRNA.

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CloneID	OBu56739
Clone ID Related Accession (Same CDS sequence)	XM_025271182.1
Accession Version	XM_025271182.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	tight junction protein ZO-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC 
GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG 
TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA 
CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC 
ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG 
AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTAAAGG GGGGGCCTGC AGAAGGACAG 
CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT 
GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATTAC CATTCGAAGG 
AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA 
GAGGACAGTT ACGACGAGGA GGCGCATGAC CCCAGAAGTG GCCGTGGGGC CCTGGCAAAC 
AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC 
CCGAGGTCTG ACAGGCGGTC TGTGGCCTCC AGTCAGCCTG CCAGACCCAC CAAAGTCACC 
CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG 
ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG 
CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT 
ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCGGGTC GATCCCACGA CCGGCCTCCC 
CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ACACACCTAA AACAGTGGAA 
GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG 
TATGCCCAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGTGTCCTG 
CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG 
AAAGGAGATA GTGTGGGTCT TCGGCTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT 
GGTGTTCTCG AAGACAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC 
AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT 
GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAAAAAGA TGTTTATCGT 
CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG 
AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT 
CTGTACAATG GGAAGCTGGG GTCCTGGCTC GCCATTCGGA TTGGCAAAAA CCATAAAGAG 
GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT 
ACACTTCCCA AGACAGCAGG GGGAGACCGC GCTGACTTCT GGAGGTTCCG CGGTCTGCGC 
AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC 
AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC 
ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAGCTGG CAAGAGAGGA GCCAGATATT 
TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT 
ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT GCTGGATGTA 
ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATAGT GGTGTTCCTC 
AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGGATGA GGTTATGTCC AGAATCTCGG 
AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT 
TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ATTGAAAGAA 
GCAATCCAGC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT 
ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT 
GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGATACA 
GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT 
CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG 
CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT 
AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGA AAGCAGAAGC CTCATCTCCA 
GGCCCTTACC TTTCGCCTGA AACAAACCCG GCACCATCAG CCTCTGCTGT TAATCCTCAC 
GTAGACTTAA CTAATGTCAG ACTGGAGGAG CCGAGCCCAG CTCCCCCTTC CTCTGACTCA 
CCACACGCCG ACTCTTTACG AGCTCCAGGC ACTGAGGCCG CTCACGTGCA GCTCAGAGGT 
CCAGAGCCGT CCCTGTCGTC GCATGTGGGC CCAGCAAAGG TGTACAGGAA GGACCCCTAC 
CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGCGGT TCACCCTGGG 
CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA 
CAGGCTGGCA GGGAGTTGGA GCAGCCTGCA TACAGATATG ATGCCTCTAG CTACCCGGAG 
CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGATG ACCGCCTCCT CACCTACGAG 
GAGCAGTGGC CCTACTACGA CGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG 
CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTTCCCCGTT TTGAGGAGCC AGTGCCCCTG 
TCCTATGAAG GCCGAGCGCA CTATGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA 
GAGCCTCCTG CCCCTGCCCC TGCCCCTGGG TACGACGTGC ACGGCAGGTA CCGGCCTGAC 
GCGCAGCCCT ACTCCACTGC TGGCCCCAAG GTGCCCCCTG AGCCCAAGCC GTACTTGGAC 
CAGTACCCTC GGAGTTACGA GCCAGCGCCA GCCCAGGGCT TCCCCTCCAG GGCAGGCCCA 
GGCCACTACG AGCCTCTTCA CAGCACCACG CTCGCTCCAC CACTTGGCCC CTCGTCCCAG 
CACAAGCCCG AAGTCCCGCC CACAGGCTCC AAACCCCTGC CTCCACCCCC AGCTCCAGCT 
GAGGAGGAGG AAGACCCAGC CATGAAGCCA CAATCCGTGC TCACCAGAGT GAAGATGTTT 
GAGAACAAGA GGTCTGCTTC CATGGAGAAC AGGAAGGATG AGAGCCATTC CACTAGTTTC 
AAGCCCTCAG AAGTCACGTC AAAACCGGCA GGTGCTCCTG TCCCAGGTCC CAAAGCCCCT 
GCTCCGGGTC AGCTCGGTGA ACACGACAGA GCAGCGCATA GGATTCCTGA ACCACAGAAG 
CCTCACGTGA AGCCACCTGA AGACATTGTT CGGGCAAATC ACTATGACCC CGAGGAGGAT 
GAGGAGTACT ACCGGAAGCA GCTCTCCTAC TTCGATCGGA GAAGCTTTGA GGCCAAGCCC 
CCCACCCACA TCCCCACTAG CCACCTCTCC GAGCCCACCA GGCCGGCTCA CCCCCAATCT 
CAGCCACAGT TTGCCGGTTA CTCTTCCAAG GGGAAGGCTG CTGAGGCCGA AGCTGTGGAC 
AGGTCATTCG GGGAGAAGCG CTATGACCCG CTGCAGGCGA CGCCCCCCCC TCCGCCGCTG 
CCCGCTCAGT ACAGCCAGAC TGCTCAACCC AGCCCCAACA CGGCTCTCGG CCTCCACGCA 
CACACCAAGG GGGCGCACGG TGAAGGTAAT GCCATGTCAT TGGATTTTCA GAACTCGTTA 
GTGTCCAAAC CAGACCCACC TCCATCTCAG AATAAGCCAG CAGCTTACAG ACCAGCCCCT 
CGAGAGGATG CTGTCCAGTC TGCCTTCTAC CCACAAAAGA GTTTCCCAGA GAAAGCCCCA 
GCTAATGGGG TCGAGCAGAC GCAGAAAACC GTCACGCCAG CATACAATCG ATTCACACCA 
AAACCATACA CAAGCTCTGC ACGGCCGTTT GAACGCAAGT TTGAAAGCCC TAAATTCAAC 
CACAATCTCT TGCCGAATGA AACCGCACAC AAACCTGACC TGTCCTCCAA AGCCCCTGCT 
TCTCCGAAAA CCCTCCTGAA GTCTGCACAG CCTCCTGAGT TTGACAGTGG TGTGGAGACC 
TTCTCTGTCC ACACAGACAA CAAGCTGAAA TACCAAGTAA ACAGTGTTAG CACGGGGCCG 
AGAGCAGTGC CCGTGAGTCC CTCAGCCGTG GAGGAAGATG AAGATGAAGA CGGTCACACA 
GTGGTGGCTA CTGCCCGCGG CGTGTTTAAC AGCAATGGTG GGGTGCTGAG CTCCATTGAG 
ACCGGTGTCA GCATCATCAT CCCCCAGGGA GCCATTCCTG AGGGGGTGGA ACAGGAGATC 
TACTTCAAGG TCTGCCGCGA CAACAGCATC CTGCCGCCTT TGGACAAAGA GAAGGGTGAG 
ACCCTGCTGA GCCCCCTAGT GATGTGTGGG CCCCATGGCC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCC TACCACACTG TGCGTCCATG ACTCCTGACG GTGATCCTAA AACCTGGCAA 
AACAAGTGTC TTCCTGGAGA TCCAAATTAT CTTGTTGGAG CAAACTGTGT TTCTGTCCTG 
ATTGACCACT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025126967.1
CDS	37..5571
Translation	MRRLEGLCLRRRRRRGPGRERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRRLWVTPSSENPSGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTTMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVNRPDPEPVSENEEDSYDEEAHDPRSGRGALANRRSEKSWGRDRSASRERSLSPRSDRRSVASSQPARPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSADASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTVEEVVVERNEKQAPNLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHPDHQAYPPYSPQAQPQPGHRIDSPGFKTASQQKAEASSPGPYLSPETNPAPSASAVNPHVDLTNVRLEEPSPAPPSSDSPHADSLRAPGTEAAHVQLRGPEPSLSSHVGPAKVYRKDPYPEEMMRQNHVLKQPAAVHPGQRPDKEQGLSYEPQPPYTERQAGRELEQPAYRYDASSYPEQFARNYDHRLRYDDRLLTYEEQWPYYDDKQPYQPRPLDGQPPAEPTERGYFPRFEEPVPLSYEGRAHYDPLPRTSTLRHEEPPAPAPAPGYDVHGRYRPDAQPYSTAGPKVPPEPKPYLDQYPRSYEPAPAQGFPSRAGPGHYEPLHSTTLAPPLGPSSQHKPEVPPTGSKPLPPPPAPAEEEEDPAMKPQSVLTRVKMFENKRSASMENRKDESHSTSFKPSEVTSKPAGAPVPGPKAPAPGQLGEHDRAAHRIPEPQKPHVKPPEDIVRANHYDPEEDEEYYRKQLSYFDRRSFEAKPPTHIPTSHLSEPTRPAHPQSQPQFAGYSSKGKAAEAEAVDRSFGEKRYDPLQATPPPPPLPAQYSQTAQPSPNTALGLHAHTKGAHGEGNAMSLDFQNSLVSKPDPPPSQNKPAAYRPAPREDAVQSAFYPQKSFPEKAPANGVEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPNETAHKPDLSSKAPASPKTLLKSAQPPEFDSGVETFSVHTDNKLKYQVNSVSTGPRAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_025271182.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025271182.1

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC 
GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG 
TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA 
CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC 
ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG 
AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTAAAGG GGGGGCCTGC AGAAGGACAG 
CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT 
GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATTAC CATTCGAAGG 
AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA 
GAGGACAGTT ACGACGAGGA GGCGCATGAC CCCAGAAGTG GCCGTGGGGC CCTGGCAAAC 
AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC 
CCGAGGTCTG ACAGGCGGTC TGTGGCCTCC AGTCAGCCTG CCAGACCCAC CAAAGTCACC 
CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG 
ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG 
CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT 
ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCGGGTC GATCCCACGA CCGGCCTCCC 
CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ACACACCTAA AACAGTGGAA 
GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG 
TATGCCCAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGTGTCCTG 
CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG 
AAAGGAGATA GTGTGGGTCT TCGGCTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT 
GGTGTTCTCG AAGACAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC 
AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT 
GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAAAAAGA TGTTTATCGT 
CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG 
AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT 
CTGTACAATG GGAAGCTGGG GTCCTGGCTC GCCATTCGGA TTGGCAAAAA CCATAAAGAG 
GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT 
ACACTTCCCA AGACAGCAGG GGGAGACCGC GCTGACTTCT GGAGGTTCCG CGGTCTGCGC 
AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC 
AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC 
ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAGCTGG CAAGAGAGGA GCCAGATATT 
TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT 
ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT GCTGGATGTA 
ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATAGT GGTGTTCCTC 
AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGGATGA GGTTATGTCC AGAATCTCGG 
AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT 
TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ATTGAAAGAA 
GCAATCCAGC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT 
ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT 
GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGATACA 
GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT 
CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG 
CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT 
AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGA AAGCAGAAGC CTCATCTCCA 
GGCCCTTACC TTTCGCCTGA AACAAACCCG GCACCATCAG CCTCTGCTGT TAATCCTCAC 
GTAGACTTAA CTAATGTCAG ACTGGAGGAG CCGAGCCCAG CTCCCCCTTC CTCTGACTCA 
CCACACGCCG ACTCTTTACG AGCTCCAGGC ACTGAGGCCG CTCACGTGCA GCTCAGAGGT 
CCAGAGCCGT CCCTGTCGTC GCATGTGGGC CCAGCAAAGG TGTACAGGAA GGACCCCTAC 
CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGCGGT TCACCCTGGG 
CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA 
CAGGCTGGCA GGGAGTTGGA GCAGCCTGCA TACAGATATG ATGCCTCTAG CTACCCGGAG 
CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGATG ACCGCCTCCT CACCTACGAG 
GAGCAGTGGC CCTACTACGA CGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG 
CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTTCCCCGTT TTGAGGAGCC AGTGCCCCTG 
TCCTATGAAG GCCGAGCGCA CTATGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA 
GAGCCTCCTG CCCCTGCCCC TGCCCCTGGG TACGACGTGC ACGGCAGGTA CCGGCCTGAC 
GCGCAGCCCT ACTCCACTGC TGGCCCCAAG GTGCCCCCTG AGCCCAAGCC GTACTTGGAC 
CAGTACCCTC GGAGTTACGA GCCAGCGCCA GCCCAGGGCT TCCCCTCCAG GGCAGGCCCA 
GGCCACTACG AGCCTCTTCA CAGCACCACG CTCGCTCCAC CACTTGGCCC CTCGTCCCAG 
CACAAGCCCG AAGTCCCGCC CACAGGCTCC AAACCCCTGC CTCCACCCCC AGCTCCAGCT 
GAGGAGGAGG AAGACCCAGC CATGAAGCCA CAATCCGTGC TCACCAGAGT GAAGATGTTT 
GAGAACAAGA GGTCTGCTTC CATGGAGAAC AGGAAGGATG AGAGCCATTC CACTAGTTTC 
AAGCCCTCAG AAGTCACGTC AAAACCGGCA GGTGCTCCTG TCCCAGGTCC CAAAGCCCCT 
GCTCCGGGTC AGCTCGGTGA ACACGACAGA GCAGCGCATA GGATTCCTGA ACCACAGAAG 
CCTCACGTGA AGCCACCTGA AGACATTGTT CGGGCAAATC ACTATGACCC CGAGGAGGAT 
GAGGAGTACT ACCGGAAGCA GCTCTCCTAC TTCGATCGGA GAAGCTTTGA GGCCAAGCCC 
CCCACCCACA TCCCCACTAG CCACCTCTCC GAGCCCACCA GGCCGGCTCA CCCCCAATCT 
CAGCCACAGT TTGCCGGTTA CTCTTCCAAG GGGAAGGCTG CTGAGGCCGA AGCTGTGGAC 
AGGTCATTCG GGGAGAAGCG CTATGACCCG CTGCAGGCGA CGCCCCCCCC TCCGCCGCTG 
CCCGCTCAGT ACAGCCAGAC TGCTCAACCC AGCCCCAACA CGGCTCTCGG CCTCCACGCA 
CACACCAAGG GGGCGCACGG TGAAGGTAAT GCCATGTCAT TGGATTTTCA GAACTCGTTA 
GTGTCCAAAC CAGACCCACC TCCATCTCAG AATAAGCCAG CAGCTTACAG ACCAGCCCCT 
CGAGAGGATG CTGTCCAGTC TGCCTTCTAC CCACAAAAGA GTTTCCCAGA GAAAGCCCCA 
GCTAATGGGG TCGAGCAGAC GCAGAAAACC GTCACGCCAG CATACAATCG ATTCACACCA 
AAACCATACA CAAGCTCTGC ACGGCCGTTT GAACGCAAGT TTGAAAGCCC TAAATTCAAC 
CACAATCTCT TGCCGAATGA AACCGCACAC AAACCTGACC TGTCCTCCAA AGCCCCTGCT 
TCTCCGAAAA CCCTCCTGAA GTCTGCACAG CCTCCTGAGT TTGACAGTGG TGTGGAGACC 
TTCTCTGTCC ACACAGACAA CAAGCTGAAA TACCAAGTAA ACAGTGTTAG CACGGGGCCG 
AGAGCAGTGC CCGTGAGTCC CTCAGCCGTG GAGGAAGATG AAGATGAAGA CGGTCACACA 
GTGGTGGCTA CTGCCCGCGG CGTGTTTAAC AGCAATGGTG GGGTGCTGAG CTCCATTGAG 
ACCGGTGTCA GCATCATCAT CCCCCAGGGA GCCATTCCTG AGGGGGTGGA ACAGGAGATC 
TACTTCAAGG TCTGCCGCGA CAACAGCATC CTGCCGCCTT TGGACAAAGA GAAGGGTGAG 
ACCCTGCTGA GCCCCCTAGT GATGTGTGGG CCCCATGGCC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCC TACCACACTG TGCGTCCATG ACTCCTGACG GTGATCCTAA AACCTGGCAA 
AACAAGTGTC TTCCTGGAGA TCCAAATTAT CTTGTTGGAG CAAACTGTGT TTCTGTCCTG 
ATTGACCACT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_025271183.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025126968.1 Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X3, mRNA.

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CloneID	OBu56740
Clone ID Related Accession (Same CDS sequence)	XM_025271183.1
Accession Version	XM_025271183.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	tight junction protein ZO-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC 
GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG 
TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA 
CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC 
ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG 
AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTAAAGG GGGGGCCTGC AGAAGGACAG 
CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT 
GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATTAC CATTCGAAGG 
AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA 
GAGGACAGTT ACGACGAGGA GGCGCATGAC CCCAGAAGTG GCCGTGGGGC CCTGGCAAAC 
AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC 
CCGAGGTCTG ACAGGCGGTC TGTGGCCTCC AGTCAGCCTG CCAGACCCAC CAAAGTCACC 
CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG 
ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG 
CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT 
ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCGGGTC GATCCCACGA CCGGCCTCCC 
CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ACACACCTAA AACAGTGGAA 
GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG 
TATGCCCAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGTGTCCTG 
CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG 
AAAGGAGATA GTGTGGGTCT TCGGCTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT 
GGTGTTCTCG AAGACAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC 
AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT 
GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAAAAAGA TGTTTATCGT 
CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG 
AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT 
CTGTACAATG GGAAGCTGGG GTCCTGGCTC GCCATTCGGA TTGGCAAAAA CCATAAAGAG 
GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT 
ACACTTCCCA AGACAGCAGG GGGAGACCGC GCTGACTTCT GGAGGTTCCG CGGTCTGCGC 
AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC 
AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC 
ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAGCTGG CAAGAGAGGA GCCAGATATT 
TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT 
ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT GCTGGATGTA 
ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATAGT GGTGTTCCTC 
AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGGATGA GGTTATGTCC AGAATCTCGG 
AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT 
TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ATTGAAAGAA 
GCAATCCAGC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT 
ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT 
GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGATACA 
GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT 
CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG 
CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT 
AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGA AAGCAGAAGC CTCATCTCCA 
GGCCCTTACC TTTCGCCTGA AACAAACCCG GCACCATCAG CCTCTGCTGT TAATCCTCAC 
GTAGACTTAA CTAATGTCAG ACTGGAGGAG CCGAGCCCAG CTCCCCCTTC CTCTGACTCA 
CCACACGCCG ACTCTTTACG AGCTCCAGGC ACTGAGGCCG CTCACGTGCA GCTCAGAGGT 
CCAGAGCCGT CCCTGTCGTC GCATGTGGGC CCAGCAAAGG TGTACAGGAA GGACCCCTAC 
CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGCGGT TCACCCTGGG 
CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA 
CAGGCTGGCA GGGAGTTGGA GCAGCCTGCA TACAGATATG ATGCCTCTAG CTACCCGGAG 
CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGATG ACCGCCTCCT CACCTACGAG 
GAGCAGTGGC CCTACTACGA CGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG 
CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTTCCCCGTT TTGAGGAGCC AGTGCCCCTG 
TCCTATGAAG GCCGAGCGCA CTATGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA 
GAGCCTCCTG CCCCTGCCCC TGCCCCTGGG TACGACGTGC ACGGCAGGTA CCGGCCTGAC 
GCGCAGCCCT ACTCCACTGC TGGCCCCAAG GTGCCCCCTG AGCCCAAGCC GTACTTGGAC 
CAGTACCCTC GGAGTTACGA GCCAGCGCCA GCCCAGGGCT TCCCCTCCAG GGCAGGCCCA 
GGCCACTACG AGCCTCTTCA CAGCACCACG CTCGCTCCAC CACTTGGCCC CTCGTCCCAG 
CACAAGCCCG AAGTCCCGCC CACAGGCTCC AAACCCCTGC CTCCACCCCC AGCTCCAGCT 
GAGGAGGAGG AAGACCCAGC CATGAAGCCA CAATCCGTGC TCACCAGAGT GAAGATGTTT 
GAGAACAAGA GGTCTGCTTC CATGGAGAAC AGGAAGGATG AGAGCCATTC CACTAGTTTC 
AAGCCCTCAG AAGTCACGTC AAAACCGGCA GGTGCTCCTG TCCCAGGTCC CAAAGCCCCT 
GCTCCGGGTC AGCTCGGTGA ACACGACAGA GCAGCGCATA GGATTCCTGA ACCACAGAAG 
CCTCACGTGA AGCCACCTGA AGACATTGTT CGGGCAAATC ACTATGACCC CGAGGAGGAT 
GAGGAGTACT ACCGGAAGCA GCTCTCCTAC TTCGATCGGA GAAGCTTTGA GGCCAAGCCC 
CCCACCCACA TCCCCACTAG CCACCTCTCC GAGCCCACCA GGCCGGCTCA CCCCCAATCT 
CAGCCACAGT TTGCCGGTTA CTCTTCCAAG GGGAAGGCTG CTGAGGCCGA AGCTGTGGAC 
AGGTCATTCG GGGAGAAGCG CTATGACCCG CTGCAGGCGA CGCCCCCCCC TCCGCCGCTG 
CCCGCTCAGT ACAGCCAGAC TGCTCAACCC AGCCCCAACA CGGCTCTCGG CCTCCACGCA 
CACACCAAGG GGGCGCACGG TGAAGGTAAT GCCATGTCAT TGGATTTTCA GAACTCGTTA 
GTGTCCAAAC CAGACCCACC TCCATCTCAG AATAAGCCAG CAGCTTACAG ACCAGCCCCT 
CGAGAGGATG CTGTCCAGTC TGCCTTCTAC CCACAAAAGA GTTTCCCAGA GAAAGCCCCA 
GCTAATGGGG TCGAGCAGAC GCAGAAAACC GTCACGCCAG CATACAATCG ATTCACACCA 
AAACCATACA CAAGCTCTGC ACGGCCGTTT GAACGCAAGT TTGAAAGCCC TAAATTCAAC 
CACAATCTCT TGCCGAATGA AACCGCACAC AAACCTGACC TGTCCTCCAA AGCCCCTGCT 
TCTCCGAAAA CCCTCCTGAA GTCTGCACAG CCTCCTGAGT TTGACAGTGG TGTGGAGACC 
TTCTCTGTCC ACACAGACAA CAAGCTGAAA TACCAAGTAA ACAGTGTTAG CACGGGGCCG 
AGAGCAGTGC CCGTGAGTCC CTCAGCCGTG GAGGAAGATG AAGATGAAGA CGGTCACACA 
GTGGTGGCTA CTGCCCGCGG CGTGTTTAAC AGCAATGGTG GGGTGCTGAG CTCCATTGAG 
ACCGGTGTCA GCATCATCAT CCCCCAGGGA GCCATTCCTG AGGGGGTGGA ACAGGAGATC 
TACTTCAAGG TCTGCCGCGA CAACAGCATC CTGCCGCCTT TGGACAAAGA GAAGGGTGAG 
ACCCTGCTGA GCCCCCTAGT GATGTGTGGG CCCCATGGCC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCC TACCACACTG TGATCCTAAA ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT 
CCAAATTATC TTGTTGGAGC AAACTGTGTT TCTGTCCTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025126968.1
CDS	37..5550
Translation	MRRLEGLCLRRRRRRGPGRERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRRLWVTPSSENPSGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTTMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVNRPDPEPVSENEEDSYDEEAHDPRSGRGALANRRSEKSWGRDRSASRERSLSPRSDRRSVASSQPARPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSADASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTVEEVVVERNEKQAPNLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHPDHQAYPPYSPQAQPQPGHRIDSPGFKTASQQKAEASSPGPYLSPETNPAPSASAVNPHVDLTNVRLEEPSPAPPSSDSPHADSLRAPGTEAAHVQLRGPEPSLSSHVGPAKVYRKDPYPEEMMRQNHVLKQPAAVHPGQRPDKEQGLSYEPQPPYTERQAGRELEQPAYRYDASSYPEQFARNYDHRLRYDDRLLTYEEQWPYYDDKQPYQPRPLDGQPPAEPTERGYFPRFEEPVPLSYEGRAHYDPLPRTSTLRHEEPPAPAPAPGYDVHGRYRPDAQPYSTAGPKVPPEPKPYLDQYPRSYEPAPAQGFPSRAGPGHYEPLHSTTLAPPLGPSSQHKPEVPPTGSKPLPPPPAPAEEEEDPAMKPQSVLTRVKMFENKRSASMENRKDESHSTSFKPSEVTSKPAGAPVPGPKAPAPGQLGEHDRAAHRIPEPQKPHVKPPEDIVRANHYDPEEDEEYYRKQLSYFDRRSFEAKPPTHIPTSHLSEPTRPAHPQSQPQFAGYSSKGKAAEAEAVDRSFGEKRYDPLQATPPPPPLPAQYSQTAQPSPNTALGLHAHTKGAHGEGNAMSLDFQNSLVSKPDPPPSQNKPAAYRPAPREDAVQSAFYPQKSFPEKAPANGVEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPNETAHKPDLSSKAPASPKTLLKSAQPPEFDSGVETFSVHTDNKLKYQVNSVSTGPRAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_025271183.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025271183.1

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC 
GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG 
TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA 
CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC 
ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG 
AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTAAAGG GGGGGCCTGC AGAAGGACAG 
CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT 
GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATTAC CATTCGAAGG 
AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA 
GAGGACAGTT ACGACGAGGA GGCGCATGAC CCCAGAAGTG GCCGTGGGGC CCTGGCAAAC 
AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC 
CCGAGGTCTG ACAGGCGGTC TGTGGCCTCC AGTCAGCCTG CCAGACCCAC CAAAGTCACC 
CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG 
ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG 
CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT 
ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCGGGTC GATCCCACGA CCGGCCTCCC 
CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ACACACCTAA AACAGTGGAA 
GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG 
TATGCCCAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGTGTCCTG 
CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG 
AAAGGAGATA GTGTGGGTCT TCGGCTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT 
GGTGTTCTCG AAGACAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC 
AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT 
GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAAAAAGA TGTTTATCGT 
CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG 
AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT 
CTGTACAATG GGAAGCTGGG GTCCTGGCTC GCCATTCGGA TTGGCAAAAA CCATAAAGAG 
GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT 
ACACTTCCCA AGACAGCAGG GGGAGACCGC GCTGACTTCT GGAGGTTCCG CGGTCTGCGC 
AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC 
AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC 
ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAGCTGG CAAGAGAGGA GCCAGATATT 
TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT 
ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT GCTGGATGTA 
ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATAGT GGTGTTCCTC 
AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGGATGA GGTTATGTCC AGAATCTCGG 
AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT 
TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ATTGAAAGAA 
GCAATCCAGC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT 
ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT 
GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGATACA 
GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT 
CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG 
CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT 
AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGA AAGCAGAAGC CTCATCTCCA 
GGCCCTTACC TTTCGCCTGA AACAAACCCG GCACCATCAG CCTCTGCTGT TAATCCTCAC 
GTAGACTTAA CTAATGTCAG ACTGGAGGAG CCGAGCCCAG CTCCCCCTTC CTCTGACTCA 
CCACACGCCG ACTCTTTACG AGCTCCAGGC ACTGAGGCCG CTCACGTGCA GCTCAGAGGT 
CCAGAGCCGT CCCTGTCGTC GCATGTGGGC CCAGCAAAGG TGTACAGGAA GGACCCCTAC 
CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGCGGT TCACCCTGGG 
CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA 
CAGGCTGGCA GGGAGTTGGA GCAGCCTGCA TACAGATATG ATGCCTCTAG CTACCCGGAG 
CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGATG ACCGCCTCCT CACCTACGAG 
GAGCAGTGGC CCTACTACGA CGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG 
CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTTCCCCGTT TTGAGGAGCC AGTGCCCCTG 
TCCTATGAAG GCCGAGCGCA CTATGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA 
GAGCCTCCTG CCCCTGCCCC TGCCCCTGGG TACGACGTGC ACGGCAGGTA CCGGCCTGAC 
GCGCAGCCCT ACTCCACTGC TGGCCCCAAG GTGCCCCCTG AGCCCAAGCC GTACTTGGAC 
CAGTACCCTC GGAGTTACGA GCCAGCGCCA GCCCAGGGCT TCCCCTCCAG GGCAGGCCCA 
GGCCACTACG AGCCTCTTCA CAGCACCACG CTCGCTCCAC CACTTGGCCC CTCGTCCCAG 
CACAAGCCCG AAGTCCCGCC CACAGGCTCC AAACCCCTGC CTCCACCCCC AGCTCCAGCT 
GAGGAGGAGG AAGACCCAGC CATGAAGCCA CAATCCGTGC TCACCAGAGT GAAGATGTTT 
GAGAACAAGA GGTCTGCTTC CATGGAGAAC AGGAAGGATG AGAGCCATTC CACTAGTTTC 
AAGCCCTCAG AAGTCACGTC AAAACCGGCA GGTGCTCCTG TCCCAGGTCC CAAAGCCCCT 
GCTCCGGGTC AGCTCGGTGA ACACGACAGA GCAGCGCATA GGATTCCTGA ACCACAGAAG 
CCTCACGTGA AGCCACCTGA AGACATTGTT CGGGCAAATC ACTATGACCC CGAGGAGGAT 
GAGGAGTACT ACCGGAAGCA GCTCTCCTAC TTCGATCGGA GAAGCTTTGA GGCCAAGCCC 
CCCACCCACA TCCCCACTAG CCACCTCTCC GAGCCCACCA GGCCGGCTCA CCCCCAATCT 
CAGCCACAGT TTGCCGGTTA CTCTTCCAAG GGGAAGGCTG CTGAGGCCGA AGCTGTGGAC 
AGGTCATTCG GGGAGAAGCG CTATGACCCG CTGCAGGCGA CGCCCCCCCC TCCGCCGCTG 
CCCGCTCAGT ACAGCCAGAC TGCTCAACCC AGCCCCAACA CGGCTCTCGG CCTCCACGCA 
CACACCAAGG GGGCGCACGG TGAAGGTAAT GCCATGTCAT TGGATTTTCA GAACTCGTTA 
GTGTCCAAAC CAGACCCACC TCCATCTCAG AATAAGCCAG CAGCTTACAG ACCAGCCCCT 
CGAGAGGATG CTGTCCAGTC TGCCTTCTAC CCACAAAAGA GTTTCCCAGA GAAAGCCCCA 
GCTAATGGGG TCGAGCAGAC GCAGAAAACC GTCACGCCAG CATACAATCG ATTCACACCA 
AAACCATACA CAAGCTCTGC ACGGCCGTTT GAACGCAAGT TTGAAAGCCC TAAATTCAAC 
CACAATCTCT TGCCGAATGA AACCGCACAC AAACCTGACC TGTCCTCCAA AGCCCCTGCT 
TCTCCGAAAA CCCTCCTGAA GTCTGCACAG CCTCCTGAGT TTGACAGTGG TGTGGAGACC 
TTCTCTGTCC ACACAGACAA CAAGCTGAAA TACCAAGTAA ACAGTGTTAG CACGGGGCCG 
AGAGCAGTGC CCGTGAGTCC CTCAGCCGTG GAGGAAGATG AAGATGAAGA CGGTCACACA 
GTGGTGGCTA CTGCCCGCGG CGTGTTTAAC AGCAATGGTG GGGTGCTGAG CTCCATTGAG 
ACCGGTGTCA GCATCATCAT CCCCCAGGGA GCCATTCCTG AGGGGGTGGA ACAGGAGATC 
TACTTCAAGG TCTGCCGCGA CAACAGCATC CTGCCGCCTT TGGACAAAGA GAAGGGTGAG 
ACCCTGCTGA GCCCCCTAGT GATGTGTGGG CCCCATGGCC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCC TACCACACTG TGATCCTAAA ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT 
CCAAATTATC TTGTTGGAGC AAACTGTGTT TCTGTCCTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_025271184.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025126969.1 Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X4, mRNA.

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CloneID	OBu56741
Clone ID Related Accession (Same CDS sequence)	XM_025271184.1
Accession Version	XM_025271184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	tight junction protein ZO-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC 
GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG 
TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA 
CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC 
ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG 
AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTAAAGG GGGGGCCTGC AGAAGGACAG 
CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT 
GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATTAC CATTCGAAGG 
AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA 
GAGGACAGTT ACGACGAGGA GGCGCATGAC CCCAGAAGTG GCCGTGGGGC CCTGGCAAAC 
AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC 
CCGAGGTCTG ACAGGCGGTC TGTGGCCTCC AGTCAGCCTG CCAGACCCAC CAAAGTCACC 
CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG 
ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG 
CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT 
ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCGGGTC GATCCCACGA CCGGCCTCCC 
CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ACACACCTAA AACAGTGGAA 
GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG 
TATGCCCAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGTGTCCTG 
CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG 
AAAGGAGATA GTGTGGGTCT TCGGCTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT 
GGTGTTCTCG AAGACAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC 
AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT 
GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAAAAAGA TGTTTATCGT 
CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG 
AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT 
CTGTACAATG GGAAGCTGGG GTCCTGGCTC GCCATTCGGA TTGGCAAAAA CCATAAAGAG 
GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT 
ACACTTCCCA AGACAGCAGG GGGAGACCGC GCTGACTTCT GGAGGTTCCG CGGTCTGCGC 
AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC 
AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC 
ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAGCTGG CAAGAGAGGA GCCAGATATT 
TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT 
ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT GCTGGATGTA 
ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATAGT GGTGTTCCTC 
AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGGATGA GGTTATGTCC AGAATCTCGG 
AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT 
TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ATTGAAAGAA 
GCAATCCAGC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT 
ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT 
GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGATACA 
GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT 
CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG 
CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT 
AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGG TGTACAGGAA GGACCCCTAC 
CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGCGGT TCACCCTGGG 
CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA 
CAGGCTGGCA GGGAGTTGGA GCAGCCTGCA TACAGATATG ATGCCTCTAG CTACCCGGAG 
CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGATG ACCGCCTCCT CACCTACGAG 
GAGCAGTGGC CCTACTACGA CGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG 
CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTTCCCCGTT TTGAGGAGCC AGTGCCCCTG 
TCCTATGAAG GCCGAGCGCA CTATGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA 
GAGCCTCCTG CCCCTGCCCC TGCCCCTGGG TACGACGTGC ACGGCAGGTA CCGGCCTGAC 
GCGCAGCCCT ACTCCACTGC TGGCCCCAAG GTGCCCCCTG AGCCCAAGCC GTACTTGGAC 
CAGTACCCTC GGAGTTACGA GCCAGCGCCA GCCCAGGGCT TCCCCTCCAG GGCAGGCCCA 
GGCCACTACG AGCCTCTTCA CAGCACCACG CTCGCTCCAC CACTTGGCCC CTCGTCCCAG 
CACAAGCCCG AAGTCCCGCC CACAGGCTCC AAACCCCTGC CTCCACCCCC AGCTCCAGCT 
GAGGAGGAGG AAGACCCAGC CATGAAGCCA CAATCCGTGC TCACCAGAGT GAAGATGTTT 
GAGAACAAGA GGTCTGCTTC CATGGAGAAC AGGAAGGATG AGAGCCATTC CACTAGTTTC 
AAGCCCTCAG AAGTCACGTC AAAACCGGCA GGTGCTCCTG TCCCAGGTCC CAAAGCCCCT 
GCTCCGGGTC AGCTCGGTGA ACACGACAGA GCAGCGCATA GGATTCCTGA ACCACAGAAG 
CCTCACGTGA AGCCACCTGA AGACATTGTT CGGGCAAATC ACTATGACCC CGAGGAGGAT 
GAGGAGTACT ACCGGAAGCA GCTCTCCTAC TTCGATCGGA GAAGCTTTGA GGCCAAGCCC 
CCCACCCACA TCCCCACTAG CCACCTCTCC GAGCCCACCA GGCCGGCTCA CCCCCAATCT 
CAGCCACAGT TTGCCGGTTA CTCTTCCAAG GGGAAGGCTG CTGAGGCCGA AGCTGTGGAC 
AGGTCATTCG GGGAGAAGCG CTATGACCCG CTGCAGGCGA CGCCCCCCCC TCCGCCGCTG 
CCCGCTCAGT ACAGCCAGAC TGCTCAACCC AGCCCCAACA CGGCTCTCGG CCTCCACGCA 
CACACCAAGG GGGCGCACGG TGAAGGTAAT GCCATGTCAT TGGATTTTCA GAACTCGTTA 
GTGTCCAAAC CAGACCCACC TCCATCTCAG AATAAGCCAG CAGCTTACAG ACCAGCCCCT 
CGAGAGGATG CTGTCCAGTC TGCCTTCTAC CCACAAAAGA GTTTCCCAGA GAAAGCCCCA 
GCTAATGGGG TCGAGCAGAC GCAGAAAACC GTCACGCCAG CATACAATCG ATTCACACCA 
AAACCATACA CAAGCTCTGC ACGGCCGTTT GAACGCAAGT TTGAAAGCCC TAAATTCAAC 
CACAATCTCT TGCCGAATGA AACCGCACAC AAACCTGACC TGTCCTCCAA AGCCCCTGCT 
TCTCCGAAAA CCCTCCTGAA GTCTGCACAG CCTCCTGAGT TTGACAGTGG TGTGGAGACC 
TTCTCTGTCC ACACAGACAA CAAGCTGAAA TACCAAGTAA ACAGTGTTAG CACGGGGCCG 
AGAGCAGTGC CCGTGAGTCC CTCAGCCGTG GAGGAAGATG AAGATGAAGA CGGTCACACA 
GTGGTGGCTA CTGCCCGCGG CGTGTTTAAC AGCAATGGTG GGGTGCTGAG CTCCATTGAG 
ACCGGTGTCA GCATCATCAT CCCCCAGGGA GCCATTCCTG AGGGGGTGGA ACAGGAGATC 
TACTTCAAGG TCTGCCGCGA CAACAGCATC CTGCCGCCTT TGGACAAAGA GAAGGGTGAG 
ACCCTGCTGA GCCCCCTAGT GATGTGTGGG CCCCATGGCC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCC TACCACACTG TGCGTCCATG ACTCCTGACG GTTGGTCTTT TGCTCTAAAA 
TCATCCGACT CCTCGTCGGG TGATCCTAAA ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT 
CCAAATTATC TTGTTGGAGC AAACTGTGTT TCTGTCCTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025126969.1
CDS	37..5370
Translation	MRRLEGLCLRRRRRRGPGRERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRRLWVTPSSENPSGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTTMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVNRPDPEPVSENEEDSYDEEAHDPRSGRGALANRRSEKSWGRDRSASRERSLSPRSDRRSVASSQPARPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSADASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTVEEVVVERNEKQAPNLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHPDHQAYPPYSPQAQPQPGHRIDSPGFKTASQQVYRKDPYPEEMMRQNHVLKQPAAVHPGQRPDKEQGLSYEPQPPYTERQAGRELEQPAYRYDASSYPEQFARNYDHRLRYDDRLLTYEEQWPYYDDKQPYQPRPLDGQPPAEPTERGYFPRFEEPVPLSYEGRAHYDPLPRTSTLRHEEPPAPAPAPGYDVHGRYRPDAQPYSTAGPKVPPEPKPYLDQYPRSYEPAPAQGFPSRAGPGHYEPLHSTTLAPPLGPSSQHKPEVPPTGSKPLPPPPAPAEEEEDPAMKPQSVLTRVKMFENKRSASMENRKDESHSTSFKPSEVTSKPAGAPVPGPKAPAPGQLGEHDRAAHRIPEPQKPHVKPPEDIVRANHYDPEEDEEYYRKQLSYFDRRSFEAKPPTHIPTSHLSEPTRPAHPQSQPQFAGYSSKGKAAEAEAVDRSFGEKRYDPLQATPPPPPLPAQYSQTAQPSPNTALGLHAHTKGAHGEGNAMSLDFQNSLVSKPDPPPSQNKPAAYRPAPREDAVQSAFYPQKSFPEKAPANGVEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPNETAHKPDLSSKAPASPKTLLKSAQPPEFDSGVETFSVHTDNKLKYQVNSVSTGPRAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_025271184.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025271184.1

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC 
GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG 
TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA 
CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC 
ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG 
AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTAAAGG GGGGGCCTGC AGAAGGACAG 
CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT 
GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATTAC CATTCGAAGG 
AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA 
GAGGACAGTT ACGACGAGGA GGCGCATGAC CCCAGAAGTG GCCGTGGGGC CCTGGCAAAC 
AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC 
CCGAGGTCTG ACAGGCGGTC TGTGGCCTCC AGTCAGCCTG CCAGACCCAC CAAAGTCACC 
CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG 
ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG 
CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT 
ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCGGGTC GATCCCACGA CCGGCCTCCC 
CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ACACACCTAA AACAGTGGAA 
GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG 
TATGCCCAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGTGTCCTG 
CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG 
AAAGGAGATA GTGTGGGTCT TCGGCTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT 
GGTGTTCTCG AAGACAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC 
AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT 
GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAAAAAGA TGTTTATCGT 
CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG 
AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT 
CTGTACAATG GGAAGCTGGG GTCCTGGCTC GCCATTCGGA TTGGCAAAAA CCATAAAGAG 
GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT 
ACACTTCCCA AGACAGCAGG GGGAGACCGC GCTGACTTCT GGAGGTTCCG CGGTCTGCGC 
AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC 
AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC 
ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAGCTGG CAAGAGAGGA GCCAGATATT 
TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT 
ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT GCTGGATGTA 
ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATAGT GGTGTTCCTC 
AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGGATGA GGTTATGTCC AGAATCTCGG 
AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT 
TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ATTGAAAGAA 
GCAATCCAGC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT 
ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT 
GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGATACA 
GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT 
CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG 
CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT 
AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGG TGTACAGGAA GGACCCCTAC 
CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGCGGT TCACCCTGGG 
CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA 
CAGGCTGGCA GGGAGTTGGA GCAGCCTGCA TACAGATATG ATGCCTCTAG CTACCCGGAG 
CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGATG ACCGCCTCCT CACCTACGAG 
GAGCAGTGGC CCTACTACGA CGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG 
CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTTCCCCGTT TTGAGGAGCC AGTGCCCCTG 
TCCTATGAAG GCCGAGCGCA CTATGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA 
GAGCCTCCTG CCCCTGCCCC TGCCCCTGGG TACGACGTGC ACGGCAGGTA CCGGCCTGAC 
GCGCAGCCCT ACTCCACTGC TGGCCCCAAG GTGCCCCCTG AGCCCAAGCC GTACTTGGAC 
CAGTACCCTC GGAGTTACGA GCCAGCGCCA GCCCAGGGCT TCCCCTCCAG GGCAGGCCCA 
GGCCACTACG AGCCTCTTCA CAGCACCACG CTCGCTCCAC CACTTGGCCC CTCGTCCCAG 
CACAAGCCCG AAGTCCCGCC CACAGGCTCC AAACCCCTGC CTCCACCCCC AGCTCCAGCT 
GAGGAGGAGG AAGACCCAGC CATGAAGCCA CAATCCGTGC TCACCAGAGT GAAGATGTTT 
GAGAACAAGA GGTCTGCTTC CATGGAGAAC AGGAAGGATG AGAGCCATTC CACTAGTTTC 
AAGCCCTCAG AAGTCACGTC AAAACCGGCA GGTGCTCCTG TCCCAGGTCC CAAAGCCCCT 
GCTCCGGGTC AGCTCGGTGA ACACGACAGA GCAGCGCATA GGATTCCTGA ACCACAGAAG 
CCTCACGTGA AGCCACCTGA AGACATTGTT CGGGCAAATC ACTATGACCC CGAGGAGGAT 
GAGGAGTACT ACCGGAAGCA GCTCTCCTAC TTCGATCGGA GAAGCTTTGA GGCCAAGCCC 
CCCACCCACA TCCCCACTAG CCACCTCTCC GAGCCCACCA GGCCGGCTCA CCCCCAATCT 
CAGCCACAGT TTGCCGGTTA CTCTTCCAAG GGGAAGGCTG CTGAGGCCGA AGCTGTGGAC 
AGGTCATTCG GGGAGAAGCG CTATGACCCG CTGCAGGCGA CGCCCCCCCC TCCGCCGCTG 
CCCGCTCAGT ACAGCCAGAC TGCTCAACCC AGCCCCAACA CGGCTCTCGG CCTCCACGCA 
CACACCAAGG GGGCGCACGG TGAAGGTAAT GCCATGTCAT TGGATTTTCA GAACTCGTTA 
GTGTCCAAAC CAGACCCACC TCCATCTCAG AATAAGCCAG CAGCTTACAG ACCAGCCCCT 
CGAGAGGATG CTGTCCAGTC TGCCTTCTAC CCACAAAAGA GTTTCCCAGA GAAAGCCCCA 
GCTAATGGGG TCGAGCAGAC GCAGAAAACC GTCACGCCAG CATACAATCG ATTCACACCA 
AAACCATACA CAAGCTCTGC ACGGCCGTTT GAACGCAAGT TTGAAAGCCC TAAATTCAAC 
CACAATCTCT TGCCGAATGA AACCGCACAC AAACCTGACC TGTCCTCCAA AGCCCCTGCT 
TCTCCGAAAA CCCTCCTGAA GTCTGCACAG CCTCCTGAGT TTGACAGTGG TGTGGAGACC 
TTCTCTGTCC ACACAGACAA CAAGCTGAAA TACCAAGTAA ACAGTGTTAG CACGGGGCCG 
AGAGCAGTGC CCGTGAGTCC CTCAGCCGTG GAGGAAGATG AAGATGAAGA CGGTCACACA 
GTGGTGGCTA CTGCCCGCGG CGTGTTTAAC AGCAATGGTG GGGTGCTGAG CTCCATTGAG 
ACCGGTGTCA GCATCATCAT CCCCCAGGGA GCCATTCCTG AGGGGGTGGA ACAGGAGATC 
TACTTCAAGG TCTGCCGCGA CAACAGCATC CTGCCGCCTT TGGACAAAGA GAAGGGTGAG 
ACCCTGCTGA GCCCCCTAGT GATGTGTGGG CCCCATGGCC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCC TACCACACTG TGCGTCCATG ACTCCTGACG GTTGGTCTTT TGCTCTAAAA 
TCATCCGACT CCTCGTCGGG TGATCCTAAA ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT 
CCAAATTATC TTGTTGGAGC AAACTGTGTT TCTGTCCTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_025271186.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025126971.1 Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X7, mRNA.

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CloneID	OBu56742
Clone ID Related Accession (Same CDS sequence)	XM_025271186.1
Accession Version	XM_025271186.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	tight junction protein ZO-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC 
GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG 
TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA 
CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC 
ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG 
AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTAAAGG GGGGGCCTGC AGAAGGACAG 
CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT 
GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATTAC CATTCGAAGG 
AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA 
GAGGACAGTT ACGACGAGGA GGCGCATGAC CCCAGAAGTG GCCGTGGGGC CCTGGCAAAC 
AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC 
CCGAGGTCTG ACAGGCGGTC TGTGGCCTCC AGTCAGCCTG CCAGACCCAC CAAAGTCACC 
CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG 
ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG 
CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT 
ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCGGGTC GATCCCACGA CCGGCCTCCC 
CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ACACACCTAA AACAGTGGAA 
GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG 
TATGCCCAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGTGTCCTG 
CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG 
AAAGGAGATA GTGTGGGTCT TCGGCTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT 
GGTGTTCTCG AAGACAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC 
AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT 
GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAAAAAGA TGTTTATCGT 
CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG 
AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT 
CTGTACAATG GGAAGCTGGG GTCCTGGCTC GCCATTCGGA TTGGCAAAAA CCATAAAGAG 
GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT 
ACACTTCCCA AGACAGCAGG GGGAGACCGC GCTGACTTCT GGAGGTTCCG CGGTCTGCGC 
AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC 
AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC 
ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAGCTGG CAAGAGAGGA GCCAGATATT 
TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT 
ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT GCTGGATGTA 
ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATAGT GGTGTTCCTC 
AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGGATGA GGTTATGTCC AGAATCTCGG 
AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT 
TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ATTGAAAGAA 
GCAATCCAGC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT 
ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT 
GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGATACA 
GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT 
CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG 
CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT 
AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGG TGTACAGGAA GGACCCCTAC 
CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGCGGT TCACCCTGGG 
CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA 
CAGGCTGGCA GGGAGTTGGA GCAGCCTGCA TACAGATATG ATGCCTCTAG CTACCCGGAG 
CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGATG ACCGCCTCCT CACCTACGAG 
GAGCAGTGGC CCTACTACGA CGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG 
CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTTCCCCGTT TTGAGGAGCC AGTGCCCCTG 
TCCTATGAAG GCCGAGCGCA CTATGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA 
GAGCCTCCTG CCCCTGCCCC TGCCCCTGGG TACGACGTGC ACGGCAGGTA CCGGCCTGAC 
GCGCAGCCCT ACTCCACTGC TGGCCCCAAG GTGCCCCCTG AGCCCAAGCC GTACTTGGAC 
CAGTACCCTC GGAGTTACGA GCCAGCGCCA GCCCAGGGCT TCCCCTCCAG GGCAGGCCCA 
GGCCACTACG AGCCTCTTCA CAGCACCACG CTCGCTCCAC CACTTGGCCC CTCGTCCCAG 
CACAAGCCCG AAGTCCCGCC CACAGGCTCC AAACCCCTGC CTCCACCCCC AGCTCCAGCT 
GAGGAGGAGG AAGACCCAGC CATGAAGCCA CAATCCGTGC TCACCAGAGT GAAGATGTTT 
GAGAACAAGA GGTCTGCTTC CATGGAGAAC AGGAAGGATG AGAGCCATTC CACTAGTTTC 
AAGCCCTCAG AAGTCACGTC AAAACCGGCA GGTGCTCCTG TCCCAGGTCC CAAAGCCCCT 
GCTCCGGGTC AGCTCGGTGA ACACGACAGA GCAGCGCATA GGATTCCTGA ACCACAGAAG 
CCTCACGTGA AGCCACCTGA AGACATTGTT CGGGCAAATC ACTATGACCC CGAGGAGGAT 
GAGGAGTACT ACCGGAAGCA GCTCTCCTAC TTCGATCGGA GAAGCTTTGA GGCCAAGCCC 
CCCACCCACA TCCCCACTAG CCACCTCTCC GAGCCCACCA GGCCGGCTCA CCCCCAATCT 
CAGCCACAGT TTGCCGGTTA CTCTTCCAAG GGGAAGGCTG CTGAGGCCGA AGCTGTGGAC 
AGGTCATTCG GGGAGAAGCG CTATGACCCG CTGCAGGCGA CGCCCCCCCC TCCGCCGCTG 
CCCGCTCAGT ACAGCCAGAC TGCTCAACCC AGCCCCAACA CGGCTCTCGG CCTCCACGCA 
CACACCAAGG GGGCGCACGG TGAAGGTAAT GCCATGTCAT TGGATTTTCA GAACTCGTTA 
GTGTCCAAAC CAGACCCACC TCCATCTCAG AATAAGCCAG CAGCTTACAG ACCAGCCCCT 
CGAGAGGATG CTGTCCAGTC TGCCTTCTAC CCACAAAAGA GTTTCCCAGA GAAAGCCCCA 
GCTAATGGGG TCGAGCAGAC GCAGAAAACC GTCACGCCAG CATACAATCG ATTCACACCA 
AAACCATACA CAAGCTCTGC ACGGCCGTTT GAACGCAAGT TTGAAAGCCC TAAATTCAAC 
CACAATCTCT TGCCGAATGA AACCGCACAC AAACCTGACC TGTCCTCCAA AGCCCCTGCT 
TCTCCGAAAA CCCTCCTGAA GTCTGCACAG CCTCCTGAGT TTGACAGTGG TGTGGAGACC 
TTCTCTGTCC ACACAGACAA CAAGCTGAAA TACCAAGTAA ACAGTGTTAG CACGGGGCCG 
AGAGCAGTGC CCGTGAGTCC CTCAGCCGTG GAGGAAGATG AAGATGAAGA CGGTCACACA 
GTGGTGGCTA CTGCCCGCGG CGTGTTTAAC AGCAATGGTG GGGTGCTGAG CTCCATTGAG 
ACCGGTGTCA GCATCATCAT CCCCCAGGGA GCCATTCCTG AGGGGGTGGA ACAGGAGATC 
TACTTCAAGG TCTGCCGCGA CAACAGCATC CTGCCGCCTT TGGACAAAGA GAAGGGTGAG 
ACCCTGCTGA GCCCCCTAGT GATGTGTGGG CCCCATGGCC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCC TACCACACTG TGATCCTAAA ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT 
CCAAATTATC TTGTTGGAGC AAACTGTGTT TCTGTCCTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025126971.1
CDS	37..5310
Translation	MRRLEGLCLRRRRRRGPGRERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRRLWVTPSSENPSGATSSVSQGKPSLRRIKGRLHRSKSLDSIDFCELTNTTMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVNRPDPEPVSENEEDSYDEEAHDPRSGRGALANRRSEKSWGRDRSASRERSLSPRSDRRSVASSQPARPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSADASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSSGSLRSREEERISKPGAVSTPVKHADDHTPKTVEEVVVERNEKQAPNLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHPDHQAYPPYSPQAQPQPGHRIDSPGFKTASQQVYRKDPYPEEMMRQNHVLKQPAAVHPGQRPDKEQGLSYEPQPPYTERQAGRELEQPAYRYDASSYPEQFARNYDHRLRYDDRLLTYEEQWPYYDDKQPYQPRPLDGQPPAEPTERGYFPRFEEPVPLSYEGRAHYDPLPRTSTLRHEEPPAPAPAPGYDVHGRYRPDAQPYSTAGPKVPPEPKPYLDQYPRSYEPAPAQGFPSRAGPGHYEPLHSTTLAPPLGPSSQHKPEVPPTGSKPLPPPPAPAEEEEDPAMKPQSVLTRVKMFENKRSASMENRKDESHSTSFKPSEVTSKPAGAPVPGPKAPAPGQLGEHDRAAHRIPEPQKPHVKPPEDIVRANHYDPEEDEEYYRKQLSYFDRRSFEAKPPTHIPTSHLSEPTRPAHPQSQPQFAGYSSKGKAAEAEAVDRSFGEKRYDPLQATPPPPPLPAQYSQTAQPSPNTALGLHAHTKGAHGEGNAMSLDFQNSLVSKPDPPPSQNKPAAYRPAPREDAVQSAFYPQKSFPEKAPANGVEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPNETAHKPDLSSKAPASPKTLLKSAQPPEFDSGVETFSVHTDNKLKYQVNSVSTGPRAVPVSPSAVEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_025271186.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis tight junction protein 1 (TJP1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025271186.1

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGGGCCC TGGCCGCGAG 
CGGAGCGGGG ACAAGATGAA GTACCAGAAG TACCTGACGG TGCTGCAGAT GGCCATCGGC 
GTCACGCCGT CCAACCGCGG CAGCCTGCTG CCGCTCAAGA GGAGGCTGTG GGTAACGCCG 
TCCTCTGAAA ACCCCAGTGG TGCTACTTCT AGTGTCAGCC AAGGAAAGCC TTCTTTAAGA 
CGAATTAAAG GGAGACTGCA CAGGAGCAAG AGCCTCGATA GCATCGATTT CTGTGAACTC 
ACTAACACAA CAATGGAGGA AACAGCTATA TGGGAACAAC ACACGGTGAC ACTTCACAGG 
GCTCCTGGAT TTGGATTTGG AATTGCAATA TCTGGTGGAA GAGATAATCC ACATTTCCAG 
AGCGGGGAAA CCTCCATCGT GATTTCAGAT GTGCTAAAGG GGGGGCCTGC AGAAGGACAG 
CTGCAGGAAA ATGACCGGGT GGCAATGGTT AATGGAGTCT CCATGGATAA TGTTGAGCAT 
GCGTTTGCTG TTCAGCAACT AAGGAAAAGT GGGAAAAATG CAAAAATTAC CATTCGAAGG 
AAGAAGAAAG TTCAGATCCC AGTAAATCGT CCTGATCCTG AGCCTGTGTC TGAGAACGAA 
GAGGACAGTT ACGACGAGGA GGCGCATGAC CCCAGAAGTG GCCGTGGGGC CCTGGCAAAC 
AGGAGGAGCG AGAAGAGCTG GGGCCGAGAT AGGAGTGCCA GTCGGGAGAG GAGTCTGTCC 
CCGAGGTCTG ACAGGCGGTC TGTGGCCTCC AGTCAGCCTG CCAGACCCAC CAAAGTCACC 
CTGGTGAAGT CCCGGAAGAA TGAAGAATAT GGTCTTCGAT TGGCCAGCCA TATATTTGTG 
AAGGAAATTT CACAAGATAG TTTGGCAGCA AGAGATGGCA ATATTCAAGA AGGCGATGTT 
GTATTGAAGA TAAATGGTAC CGTGACGGAA AACATGTCAC TGACAGATGC GAAGACGCTG 
ATCGAGAGGT CTAAGGGCAA GTTAAAGATG GTGGTTCAGA GAGATGAGCG GGCTACCCTG 
CTGAATGTCC CTGACCTTTC TGACAGCATC CACTCTGCTG ACGCCTCCGA GAGGGACGAT 
ATTTCAGAAA TCCAGTCACT GGCATCAGAC CATTCGGGTC GATCCCACGA CCGGCCTCCC 
CGTCACAGCC GCTCTCGGTC TCCTGACCAG CGGTCCGAGC CTTCTGACCA TTCTAGACAC 
TCTCCACAGC AGCCCAGCAG TGGCAGTCTC CGGAGCAGAG AGGAAGAGAG AATTTCTAAA 
CCAGGGGCTG TCTCGACTCC GGTAAAGCAT GCAGATGATC ACACACCTAA AACAGTGGAA 
GAAGTTGTAG TTGAGCGAAA TGAGAAACAA GCACCCAATC TTCCAGAACC AAAGCCTGTG 
TATGCCCAGG TAGGACAGCC AGACGTGGAT TTACCTGTCA GTCCATCTGA TGGTGTCCTG 
CCCAACTCAA CTCATGAAGA TGGCATTCTT CGGCCCAGCA TGAAGTTAGT AAAATTCAGG 
AAAGGAGATA GTGTGGGTCT TCGGCTAGCT GGTGGAAACG ATGTTGGCAT ATTTGTAGCT 
GGTGTTCTCG AAGACAGCCC TGCTGCCAAA GAAGGCTTGG AGGAGGGCGA CCAGATCCTC 
AGGGTAAACA ATGTAGATTT CACAAATATC ATAAGAGAAG AAGCCGTCCT CTTCCTGCTT 
GACCTCCCAA AAGGAGAAGA AGTGACCATA TTGGCTCAGA AGAAAAAAGA TGTTTATCGT 
CGCATCGTAG AATCCGATGT GGGTGATTCT TTCTATATCA GAACCCATTT TGAATATGAG 
AAGGAATCTC CCTATGGACT CAGTTTTAAC AAAGGAGAGG TGTTCCGTGT CGTGGACACT 
CTGTACAATG GGAAGCTGGG GTCCTGGCTC GCCATTCGGA TTGGCAAAAA CCATAAAGAG 
GTGGAACGTG GCATCATCCC AAACAAAAAC AGAGCTGAGC AGTTAGCCAG TGTGCAGTAT 
ACACTTCCCA AGACAGCAGG GGGAGACCGC GCTGACTTCT GGAGGTTCCG CGGTCTGCGC 
AGCTCCAAGA GAAACCTGCG CAAGAGCCGA GAAGACCTCT CAGCCCAGCC AGTCCAGACC 
AAGTTCCCGG CGTATGAGAG GGTGGTTCTT CGAGAAGCTG GATTCCTGAG GCCTGTAACC 
ATCTTTGGAC CAATAGCTGA TGTTGCCAGG GAGAAGCTGG CAAGAGAGGA GCCAGATATT 
TACCAGATCG CAAAAAGTGA ACCTCGGGAT GCAGGAACCG ACCAGCGCAG CTCTGGCATT 
ATTCGCCTCC ACACAATAAA GCAGATAATA GATCAGGACA AACATGCCTT GCTGGATGTA 
ACGCCCAATG CAGTAGACCG TCTGAACTAT GCCCAGTGGT ATCCAATAGT GGTGTTCCTC 
AACCCGGACT CAAAGCAGGG TGTGAAAACA ATGCGGATGA GGTTATGTCC AGAATCTCGG 
AAAAGCGCCA GGAAGTTATA TGAGCGCTCT CATAAACTTC GTAAAAATAA TCACCATCTT 
TTTACAACTA CAATTAACTT AAATTCAATG AATGATGGCT GGTATGGTGC ATTGAAAGAA 
GCAATCCAGC AACAACAGAA CCAGTTGGTA TGGGTTTCTG AGGGAAAGGC GGATGGTGCT 
ACAAGTGATG ACCTTGATTT GCATGATGAT CGTCTGTCCT ACCTGTCAGC CCCAGGTAGT 
GAATACTCAA TGTATAGCAC GGACAGTAGA CACACTTCTG ACTATGAAGA CACAGATACA 
GAAGGCGGGG CCTACACTGA TCAAGAACTA GATGAGACCC TAAATGATGA GGTGGGGACT 
CCCCCGGAGT CTGCCATTAC CCGGTCCTCT GAGCCTGTCA GAGAGGACTC CTCTGGAATG 
CATCCTGACC ACCAGGCATA TCCTCCCTAC TCACCACAAG CGCAGCCACA GCCAGGCCAT 
AGAATAGACT CCCCTGGATT TAAAACAGCC TCTCAACAGG TGTACAGGAA GGACCCCTAC 
CCTGAGGAGA TGATGAGACA GAACCACGTC TTGAAGCAGC CGGCTGCGGT TCACCCTGGG 
CAGAGGCCAG ACAAGGAGCA AGGCCTGAGC TACGAGCCGC AGCCTCCGTA CACAGAGCGA 
CAGGCTGGCA GGGAGTTGGA GCAGCCTGCA TACAGATATG ATGCCTCTAG CTACCCGGAG 
CAGTTTGCTC GGAACTATGA CCATCGTCTG CGCTATGATG ACCGCCTCCT CACCTACGAG 
GAGCAGTGGC CCTACTACGA CGACAAGCAG CCTTACCAGC CCCGGCCTCT TGATGGTCAG 
CCTCCCGCGG AGCCAACAGA ACGAGGGTAT TTTCCCCGTT TTGAGGAGCC AGTGCCCCTG 
TCCTATGAAG GCCGAGCGCA CTATGACCCA CTGCCCAGGA CCTCCACCTT GCGGCACGAA 
GAGCCTCCTG CCCCTGCCCC TGCCCCTGGG TACGACGTGC ACGGCAGGTA CCGGCCTGAC 
GCGCAGCCCT ACTCCACTGC TGGCCCCAAG GTGCCCCCTG AGCCCAAGCC GTACTTGGAC 
CAGTACCCTC GGAGTTACGA GCCAGCGCCA GCCCAGGGCT TCCCCTCCAG GGCAGGCCCA 
GGCCACTACG AGCCTCTTCA CAGCACCACG CTCGCTCCAC CACTTGGCCC CTCGTCCCAG 
CACAAGCCCG AAGTCCCGCC CACAGGCTCC AAACCCCTGC CTCCACCCCC AGCTCCAGCT 
GAGGAGGAGG AAGACCCAGC CATGAAGCCA CAATCCGTGC TCACCAGAGT GAAGATGTTT 
GAGAACAAGA GGTCTGCTTC CATGGAGAAC AGGAAGGATG AGAGCCATTC CACTAGTTTC 
AAGCCCTCAG AAGTCACGTC AAAACCGGCA GGTGCTCCTG TCCCAGGTCC CAAAGCCCCT 
GCTCCGGGTC AGCTCGGTGA ACACGACAGA GCAGCGCATA GGATTCCTGA ACCACAGAAG 
CCTCACGTGA AGCCACCTGA AGACATTGTT CGGGCAAATC ACTATGACCC CGAGGAGGAT 
GAGGAGTACT ACCGGAAGCA GCTCTCCTAC TTCGATCGGA GAAGCTTTGA GGCCAAGCCC 
CCCACCCACA TCCCCACTAG CCACCTCTCC GAGCCCACCA GGCCGGCTCA CCCCCAATCT 
CAGCCACAGT TTGCCGGTTA CTCTTCCAAG GGGAAGGCTG CTGAGGCCGA AGCTGTGGAC 
AGGTCATTCG GGGAGAAGCG CTATGACCCG CTGCAGGCGA CGCCCCCCCC TCCGCCGCTG 
CCCGCTCAGT ACAGCCAGAC TGCTCAACCC AGCCCCAACA CGGCTCTCGG CCTCCACGCA 
CACACCAAGG GGGCGCACGG TGAAGGTAAT GCCATGTCAT TGGATTTTCA GAACTCGTTA 
GTGTCCAAAC CAGACCCACC TCCATCTCAG AATAAGCCAG CAGCTTACAG ACCAGCCCCT 
CGAGAGGATG CTGTCCAGTC TGCCTTCTAC CCACAAAAGA GTTTCCCAGA GAAAGCCCCA 
GCTAATGGGG TCGAGCAGAC GCAGAAAACC GTCACGCCAG CATACAATCG ATTCACACCA 
AAACCATACA CAAGCTCTGC ACGGCCGTTT GAACGCAAGT TTGAAAGCCC TAAATTCAAC 
CACAATCTCT TGCCGAATGA AACCGCACAC AAACCTGACC TGTCCTCCAA AGCCCCTGCT 
TCTCCGAAAA CCCTCCTGAA GTCTGCACAG CCTCCTGAGT TTGACAGTGG TGTGGAGACC 
TTCTCTGTCC ACACAGACAA CAAGCTGAAA TACCAAGTAA ACAGTGTTAG CACGGGGCCG 
AGAGCAGTGC CCGTGAGTCC CTCAGCCGTG GAGGAAGATG AAGATGAAGA CGGTCACACA 
GTGGTGGCTA CTGCCCGCGG CGTGTTTAAC AGCAATGGTG GGGTGCTGAG CTCCATTGAG 
ACCGGTGTCA GCATCATCAT CCCCCAGGGA GCCATTCCTG AGGGGGTGGA ACAGGAGATC 
TACTTCAAGG TCTGCCGCGA CAACAGCATC CTGCCGCCTT TGGACAAAGA GAAGGGTGAG 
ACCCTGCTGA GCCCCCTAGT GATGTGTGGG CCCCATGGCC TCAAGTTCCT GAAGCCCGTG 
GAGCTGCGCC TACCACACTG TGATCCTAAA ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT 
CCAAATTATC TTGTTGGAGC AAACTGTGTT TCTGTCCTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TJP1
Entrez Gene ID	102402627
Full Name	tight junction protein 1
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TJP1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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