LOC102402539 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC102402539
Entrez Gene ID	102402539
Full Name	alpha-2-macroglobulin
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025283184.1	XP_025138969.1	alpha-2-macroglobulin isoform X2
XM_006069786.1	XP_006069848.1	alpha-2-macroglobulin isoform X3
XM_006069784.1	XP_006069846.1	alpha-2-macroglobulin isoform X1
XM_006069785.1	XP_006069847.1	alpha-2-macroglobulin isoform X2
XM_006069786.2	XP_006069848.1	alpha-2-macroglobulin isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC102402539 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC102402539 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu24504	XM_006069784.1 Latest version!	Bubalus bubalis alpha-2-macroglobulin (A2M), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu24505	XM_006069785.1 Latest version!	Bubalus bubalis alpha-2-macroglobulin (A2M), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu24506	XM_006069786.2 Latest version!	Bubalus bubalis alpha-2-macroglobulin (LOC102402539), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu64113	XM_025283184.1 Latest version!	Bubalus bubalis alpha-2-macroglobulin (LOC102402539), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OBu24506	XM_006069786.1	Bubalus bubalis alpha-2-macroglobulin (A2M), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC102402539 ( XM_006069784.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006069846.1 Bubalus bubalis alpha-2-macroglobulin (A2M), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu24504
Clone ID Related Accession (Same CDS sequence)	XM_006069784.1
Accession Version	XM_006069784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	alpha-2-macroglobulin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785373.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAG GCAGACTTCT TCACTCATGT CTCCTGCTAC TTCTTGTCAT TCGGTTTGCT 
ACCAGCACCT CAAATTCTGT GGAACCGCAA TACATGGTGC TGGTCCCCTC CCTGCTCCAT 
ACTGGGACCC CTGAGAAGGG CTGCCTCCTT CTGAGCCACC TGAATGAGAC GGTGACCGTC 
AGCGCCTCCC TGGAGTCCGT CAGAGAGAAC AGGAGCCTCT TCACTGACGT GGTGGCAGAG 
AAGGACTTAT TCCACTGTGT CTCCTTCACT CTCCCAAGAT CTCCAACCAG GCAAGAGGTA 
ATGTTCCTTA CCATCCAAGT AAAAGGACCA ACCCAAGAAT TCAAGAAGCG GACCACAGTG 
TTGGTTAAAA ACGAAGAGAG CCTGGTCTTT GTCCAGACAG ACAAACCCAT CTATAAACCA 
GAGCAGACAG TGAACTTTCG TATTGTCTTG TTGGATGAAA GCTTTCACCC CCTGAATGAG 
TTGGTTCCAC TAGTATATGT TGAGGACCCC AAAGGAAATC GCATCGCACA GTGGCAGAAC 
CTCGAGGTAG AGAATGGCCT CCAGCAGCTG ACCTTCCCCC TCTCATCAGA GCCCTTTCAG 
GGCTCCTATA AGGTGGTGGT ACAGAAGGGA TCAGGTGGGA CTGCAGAGCA CCCCTTCACC 
GTGGAGGAAT TCGTGCTTCC TAAGTTTGAA GTACAAGTAA GAATGCCAAA GATAATCACC 
ATCTTGGAAG AAGAGGTGAA AGTGTCTGTG TGCGGCCTAT ACACTTATGG GAAGCCTGTC 
CCAGGACGTG TGACTATGAA CATGTGCAGA AAGTACAGGA ACCCTTCTAA TTGTTATGGT 
GAAGAATCCA ATGCTGTCTG TGAGAAGTTC AGTGGACAGC TGAACAATGA GGGCTGCTTC 
TCTCAGCAAG TAAACACCAA GATCTTCCAG ATGAAGAGAC AAGAGTTTGA AATGAAAATC 
GAAGTGGAAG CCAAGATCCA AGAAGAAGGA ACAGAGGTGG AATTAACTGG AAAGGGGGCC 
ACTGAAATAA CAACAACCAT CACCAAACTC TCATTTGTCA CAGTGGACTC AAACCTTAGA 
CGAGGAATTC CTTTCACGGG GCAGGTGCTC CTAGTGGACG GGAAAGGTGT CCCCATGCCA 
AATAAAGTCA TCTTCATCAC AGCAAACGAA GCCAACCACA ACTCTAACAC CACCACTGAT 
GAGCACGGCC TGGCACAGTT CTCCATCACC ACCACCAACA TCAAGGGCAC CTCCCTCAGT 
ATCAGGGTCA AATACAAGGA TCACAGCCCC TGTTACGGCT ACCAGTGGCT GTCAGAAGAC 
CATCAAGACG CTTATCACAG TGCTAATCTT GTGTTCTCCC AAAGCAACAG CTTTGTCTAC 
CTGGAGCCTT TGCCTCGTAA ACTGCCCTGT GGCAAAACTC AGACAGTCCA GGCACATTAT 
GTGCTGAAGG GAGAGGTCCT AAAGGATCTA AAGGAGCTCG TGTTCTATTA CCTGATAATG 
GCAAAAGGAG GCATTGTCCG ATCTGGGACT CACACACTGC CCGTGGAGCA GGGAGAGATG 
CAAGGCCACT TTTCCATACC AGTCCCTGTG GAGTCAGACA TTGCTCCTGT CGCTAGGCTG 
CTCATCTATG CCATTTTACC TGATGGAGAA GTGGTAGGGG ATTCTGCAAG ATATGAAGTT 
GAACAATGTC TGAGCAACAA GGTGGGTTTG AACTTCAGCC CCGGACAAAG TTTCCCAGCC 
TCCCAGGCCC ACCTTCGAGT CACCGCTTCC CCTCAGTCCC TCTGCGCCCT CCGCGCCGTG 
GACCAAAGTG TGCTGCTCAT GAGGCCCGAA GCCGAGCTCT CAGCAGCCAC GGTTTATAAC 
CTGCTACCAG TCAAGGACCT CAGCAGTTTC CCCTCAAGTG TAAATCAACA GGAGGAAGAC 
AGTGAAGGCT GCATCAGTCG TGACAATGTC TATATCAATG GAATCATGTA TTTCCCAGTA 
TCAAATACAA ATGAAAAAGA TATGTATAGC TTCCTGCAGG ATATGGGCTT GAAGGCATTT 
ACCAACTCAA AGATTCATAA ACCCAAGATA TGTCCACAGC CTGAAGAACA TAGAGTACAG 
CATCACACAC TATTAGCTTC TCCAGTCAGA GCTGAAATGG GAAGAGACAG TAATTTTGTG 
CGTTTTGACG ATACTTCAGA GCCTCCCACA GAGACTGTAC GAAAGTACTT CCCTGAGACA 
TGGATCTGGG ACTTGGTGGT TGTAAGCTCT TCAGGTGTGC ATGAAGTAGA AGTAACAGTC 
CCTGATACCA TCACTGAGTG GAAGGCAGGG GCCCTCTGCC TGTCCAGAGA CACTGGACTT 
GGTCTCTCTC CGACCGCCTC CCTCCGAGTC TTCCAGCCCT TCTTTGTGGA GCTTACAATG 
CCCTACTCTG TGATCCGTGG AGAGGCCTTC ACACTCAAGG CCACAGTGTT AAACTACCTT 
CCCAAATGCA TCCGGGTCAG TGTGCATCTA GAAGCCTCTC CTGCATTCCT AGCTGTTCCA 
GAAAAGGAAC AAGAGACTTA CTGCATCTGT GAAAATGGCC GGCAGACTGT GTCCTGGGCG 
GTAACTCCAA AGTCCTTAGG GAATGTGAAT TTCACAGTGA GTGCAGAGGC AGTGGAGTCT 
CAAGAGCTGT GTGGGAGTGA AGTGCCTGTG GTCCCTGAGC ATGGAAGGAA AGACACAATC 
ATCAAGCCCC TATTGGTTGA ACCTGAAGGA CTAGAGAAGG AAGTAATATT CAACTCCCTG 
CTTTGTCCAT CAGGTGCCAA GGTGTCTGAA TCATTATCTC TGAAGCTGCC AGCAAATGTG 
GTGGAAGAAT CTGCCCGAGC CTCCTTCTCA GTTTTAGGAG ACATATTAGG CTCTGCCATG 
AGAAACACCC AAAATCTCCT CCAAATGCCC TATGGTTGTG GAGAACAGAA TATGGCCCTC 
TTTGCCCCTA ACATCTATGT TCTGGATTAT CTAAATGAGA CACAGCAGCT GACTGCAGAG 
CTCAAGTCCA AGGCTATCCT CTACCTCAAC ACTGGTTACC AGAGACAGCT ACTCTACAAG 
CACTTCGATG GCTCTTACAG CACCTTTGGG GAGCATCATG GCAACAGTGA GGGCAATACT 
TGGCTCACGG CCTTTGTACT GAAGAGTTTT GCCCAGGCTC GAGGCTATAT TTTCATTGAT 
GAAGCACATA TTACTGAAGC TCTCACCTGG CTGGCCCAAA AGCAGAAGAG CAATGGCTGT 
TTCAGGAGTT CTGGGTCTCT GCTTAACAAT GCCCTCAAGG GTGGGGTGGA TGATGAAGTG 
ACCCTCTCTG CCTACATCAC CATCGCCCTT CTAGAGATGC CTCTCCCTGT CACACACCCT 
GTTGTCCGCA ATGCCCTGTT CTGCCTGGAC TCAGCTTGGA AGTCAGCCAA GGAAGGATCC 
CACGGCAGCC ACGTCTACAC CAAGGCACTG TTGGCCTATG CCTTTGCCCT GGCGGGTAAC 
CAGGAAAGGA GGACAGAGGT ACTGAGGTCA CTGCATGAGG AAGCCGTGAA GGAAGATAAT 
ACCATGCACT GGACACGACC TCAGAAACCC AGGGCTCTCA CGGAGGACAT TTACCAACCC 
AGGGCTCCGT CTGTGGAGGT GGAGATGACG GCCTACGTGC TCCTTGCTCA GGTCACGGCC 
CAGCCGGCCC CGACCCCAGA GGACCTCGAC CCTGCCACGA GAATCGTGAA GTGGATCTCA 
AAGCAGCAGA ATTGCCAGGG GGGCTTCTCC TCCACCCAGG ACACAGTGGT AGCTCTCCAC 
GCCTTGTCCA GATACGGAGC AGCCACTTTC ACCAGCGCTA CGAAGGTTGC ACAGGTGACC 
ATCCAGTCTT CAGGGACATT TTCCACAAAA TTCCAAGTGG AAAACAGCAA CCGCCTGTTA 
CTACAGCAGG TTTCACTGCC AGAAGTGCCT GGAGAGTACA GCATGTCAGT CACAGGAGAA 
GGATGTGTTT ACCTCCAGAC ATCTTTGAAA TACAATATTC TCCCGAAAAA AGACGAGTTC 
CCATTTGCTT TGGAGGTGCA TACTCTGCCC CAAACTTGTG ATGGACCCAA AGCCCACACC 
AGCTTCCAGA TCTCACTGAG TGTCAGTTAT ATTGGAAGCC GTCCAGCCTC CAATATGGCA 
ATTGTTGATG TGAAGATGGT ATCTGGCTTC ATTCCCTTGA AACCAACAGT GAAAATGCTA 
GAAAGATCTA ATGTGAGCCG AACAGAAGTT AGCAACAATC ATGTTTTGAT TTATCTGGAT 
AAGGTGACAG ATGAGACCCT GACCTTGACC TTCACAGTTC TGCAAGACAT CCCAGTAAGG 
GATCTGAAAC CGGCCATAGT GAAAGTCTAT GACTATTATG AGACGGATGA GTTTGCAGTT 
GCTGAGTACA GTGCTCCGTG CAGCAAAGGT AAGCAAACCT CCTTCCAGAT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006069846.1
CDS	60..4496
Translation	MGEGRLLHSCLLLLLVIRFATSTSNSVEPQYMVLVPSLLHTGTPEKGCLLLSHLNETVTVSASLESVRENRSLFTDVVAEKDLFHCVSFTLPRSPTRQEVMFLTIQVKGPTQEFKKRTTVLVKNEESLVFVQTDKPIYKPEQTVNFRIVLLDESFHPLNELVPLVYVEDPKGNRIAQWQNLEVENGLQQLTFPLSSEPFQGSYKVVVQKGSGGTAEHPFTVEEFVLPKFEVQVRMPKIITILEEEVKVSVCGLYTYGKPVPGRVTMNMCRKYRNPSNCYGEESNAVCEKFSGQLNNEGCFSQQVNTKIFQMKRQEFEMKIEVEAKIQEEGTEVELTGKGATEITTTITKLSFVTVDSNLRRGIPFTGQVLLVDGKGVPMPNKVIFITANEANHNSNTTTDEHGLAQFSITTTNIKGTSLSIRVKYKDHSPCYGYQWLSEDHQDAYHSANLVFSQSNSFVYLEPLPRKLPCGKTQTVQAHYVLKGEVLKDLKELVFYYLIMAKGGIVRSGTHTLPVEQGEMQGHFSIPVPVESDIAPVARLLIYAILPDGEVVGDSARYEVEQCLSNKVGLNFSPGQSFPASQAHLRVTASPQSLCALRAVDQSVLLMRPEAELSAATVYNLLPVKDLSSFPSSVNQQEEDSEGCISRDNVYINGIMYFPVSNTNEKDMYSFLQDMGLKAFTNSKIHKPKICPQPEEHRVQHHTLLASPVRAEMGRDSNFVRFDDTSEPPTETVRKYFPETWIWDLVVVSSSGVHEVEVTVPDTITEWKAGALCLSRDTGLGLSPTASLRVFQPFFVELTMPYSVIRGEAFTLKATVLNYLPKCIRVSVHLEASPAFLAVPEKEQETYCICENGRQTVSWAVTPKSLGNVNFTVSAEAVESQELCGSEVPVVPEHGRKDTIIKPLLVEPEGLEKEVIFNSLLCPSGAKVSESLSLKLPANVVEESARASFSVLGDILGSAMRNTQNLLQMPYGCGEQNMALFAPNIYVLDYLNETQQLTAELKSKAILYLNTGYQRQLLYKHFDGSYSTFGEHHGNSEGNTWLTAFVLKSFAQARGYIFIDEAHITEALTWLAQKQKSNGCFRSSGSLLNNALKGGVDDEVTLSAYITIALLEMPLPVTHPVVRNALFCLDSAWKSAKEGSHGSHVYTKALLAYAFALAGNQERRTEVLRSLHEEAVKEDNTMHWTRPQKPRALTEDIYQPRAPSVEVEMTAYVLLAQVTAQPAPTPEDLDPATRIVKWISKQQNCQGGFSSTQDTVVALHALSRYGAATFTSATKVAQVTIQSSGTFSTKFQVENSNRLLLQQVSLPEVPGEYSMSVTGEGCVYLQTSLKYNILPKKDEFPFALEVHTLPQTCDGPKAHTSFQISLSVSYIGSRPASNMAIVDVKMVSGFIPLKPTVKMLERSNVSRTEVSNNHVLIYLDKVTDETLTLTFTVLQDIPVRDLKPAIVKVYDYYETDEFAVAEYSAPCSKGKQTSFQME

Target ORF information:

RefSeq Version	XM_006069784.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis alpha-2-macroglobulin (A2M), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006069784.1

ORF Insert Sequence:

ATGGGGGAAG GCAGACTTCT TCACTCATGT CTCCTGCTAC TTCTTGTCAT TCGGTTTGCT 
ACCAGCACCT CAAATTCTGT GGAACCGCAA TACATGGTGC TGGTCCCCTC CCTGCTCCAT 
ACTGGGACCC CTGAGAAGGG CTGCCTCCTT CTGAGCCACC TGAATGAGAC GGTGACCGTC 
AGCGCCTCCC TGGAGTCCGT CAGAGAGAAC AGGAGCCTCT TCACTGACGT GGTGGCAGAG 
AAGGACTTAT TCCACTGTGT CTCCTTCACT CTCCCAAGAT CTCCAACCAG GCAAGAGGTA 
ATGTTCCTTA CCATCCAAGT AAAAGGACCA ACCCAAGAAT TCAAGAAGCG GACCACAGTG 
TTGGTTAAAA ACGAAGAGAG CCTGGTCTTT GTCCAGACAG ACAAACCCAT CTATAAACCA 
GAGCAGACAG TGAACTTTCG TATTGTCTTG TTGGATGAAA GCTTTCACCC CCTGAATGAG 
TTGGTTCCAC TAGTATATGT TGAGGACCCC AAAGGAAATC GCATCGCACA GTGGCAGAAC 
CTCGAGGTAG AGAATGGCCT CCAGCAGCTG ACCTTCCCCC TCTCATCAGA GCCCTTTCAG 
GGCTCCTATA AGGTGGTGGT ACAGAAGGGA TCAGGTGGGA CTGCAGAGCA CCCCTTCACC 
GTGGAGGAAT TCGTGCTTCC TAAGTTTGAA GTACAAGTAA GAATGCCAAA GATAATCACC 
ATCTTGGAAG AAGAGGTGAA AGTGTCTGTG TGCGGCCTAT ACACTTATGG GAAGCCTGTC 
CCAGGACGTG TGACTATGAA CATGTGCAGA AAGTACAGGA ACCCTTCTAA TTGTTATGGT 
GAAGAATCCA ATGCTGTCTG TGAGAAGTTC AGTGGACAGC TGAACAATGA GGGCTGCTTC 
TCTCAGCAAG TAAACACCAA GATCTTCCAG ATGAAGAGAC AAGAGTTTGA AATGAAAATC 
GAAGTGGAAG CCAAGATCCA AGAAGAAGGA ACAGAGGTGG AATTAACTGG AAAGGGGGCC 
ACTGAAATAA CAACAACCAT CACCAAACTC TCATTTGTCA CAGTGGACTC AAACCTTAGA 
CGAGGAATTC CTTTCACGGG GCAGGTGCTC CTAGTGGACG GGAAAGGTGT CCCCATGCCA 
AATAAAGTCA TCTTCATCAC AGCAAACGAA GCCAACCACA ACTCTAACAC CACCACTGAT 
GAGCACGGCC TGGCACAGTT CTCCATCACC ACCACCAACA TCAAGGGCAC CTCCCTCAGT 
ATCAGGGTCA AATACAAGGA TCACAGCCCC TGTTACGGCT ACCAGTGGCT GTCAGAAGAC 
CATCAAGACG CTTATCACAG TGCTAATCTT GTGTTCTCCC AAAGCAACAG CTTTGTCTAC 
CTGGAGCCTT TGCCTCGTAA ACTGCCCTGT GGCAAAACTC AGACAGTCCA GGCACATTAT 
GTGCTGAAGG GAGAGGTCCT AAAGGATCTA AAGGAGCTCG TGTTCTATTA CCTGATAATG 
GCAAAAGGAG GCATTGTCCG ATCTGGGACT CACACACTGC CCGTGGAGCA GGGAGAGATG 
CAAGGCCACT TTTCCATACC AGTCCCTGTG GAGTCAGACA TTGCTCCTGT CGCTAGGCTG 
CTCATCTATG CCATTTTACC TGATGGAGAA GTGGTAGGGG ATTCTGCAAG ATATGAAGTT 
GAACAATGTC TGAGCAACAA GGTGGGTTTG AACTTCAGCC CCGGACAAAG TTTCCCAGCC 
TCCCAGGCCC ACCTTCGAGT CACCGCTTCC CCTCAGTCCC TCTGCGCCCT CCGCGCCGTG 
GACCAAAGTG TGCTGCTCAT GAGGCCCGAA GCCGAGCTCT CAGCAGCCAC GGTTTATAAC 
CTGCTACCAG TCAAGGACCT CAGCAGTTTC CCCTCAAGTG TAAATCAACA GGAGGAAGAC 
AGTGAAGGCT GCATCAGTCG TGACAATGTC TATATCAATG GAATCATGTA TTTCCCAGTA 
TCAAATACAA ATGAAAAAGA TATGTATAGC TTCCTGCAGG ATATGGGCTT GAAGGCATTT 
ACCAACTCAA AGATTCATAA ACCCAAGATA TGTCCACAGC CTGAAGAACA TAGAGTACAG 
CATCACACAC TATTAGCTTC TCCAGTCAGA GCTGAAATGG GAAGAGACAG TAATTTTGTG 
CGTTTTGACG ATACTTCAGA GCCTCCCACA GAGACTGTAC GAAAGTACTT CCCTGAGACA 
TGGATCTGGG ACTTGGTGGT TGTAAGCTCT TCAGGTGTGC ATGAAGTAGA AGTAACAGTC 
CCTGATACCA TCACTGAGTG GAAGGCAGGG GCCCTCTGCC TGTCCAGAGA CACTGGACTT 
GGTCTCTCTC CGACCGCCTC CCTCCGAGTC TTCCAGCCCT TCTTTGTGGA GCTTACAATG 
CCCTACTCTG TGATCCGTGG AGAGGCCTTC ACACTCAAGG CCACAGTGTT AAACTACCTT 
CCCAAATGCA TCCGGGTCAG TGTGCATCTA GAAGCCTCTC CTGCATTCCT AGCTGTTCCA 
GAAAAGGAAC AAGAGACTTA CTGCATCTGT GAAAATGGCC GGCAGACTGT GTCCTGGGCG 
GTAACTCCAA AGTCCTTAGG GAATGTGAAT TTCACAGTGA GTGCAGAGGC AGTGGAGTCT 
CAAGAGCTGT GTGGGAGTGA AGTGCCTGTG GTCCCTGAGC ATGGAAGGAA AGACACAATC 
ATCAAGCCCC TATTGGTTGA ACCTGAAGGA CTAGAGAAGG AAGTAATATT CAACTCCCTG 
CTTTGTCCAT CAGGTGCCAA GGTGTCTGAA TCATTATCTC TGAAGCTGCC AGCAAATGTG 
GTGGAAGAAT CTGCCCGAGC CTCCTTCTCA GTTTTAGGAG ACATATTAGG CTCTGCCATG 
AGAAACACCC AAAATCTCCT CCAAATGCCC TATGGTTGTG GAGAACAGAA TATGGCCCTC 
TTTGCCCCTA ACATCTATGT TCTGGATTAT CTAAATGAGA CACAGCAGCT GACTGCAGAG 
CTCAAGTCCA AGGCTATCCT CTACCTCAAC ACTGGTTACC AGAGACAGCT ACTCTACAAG 
CACTTCGATG GCTCTTACAG CACCTTTGGG GAGCATCATG GCAACAGTGA GGGCAATACT 
TGGCTCACGG CCTTTGTACT GAAGAGTTTT GCCCAGGCTC GAGGCTATAT TTTCATTGAT 
GAAGCACATA TTACTGAAGC TCTCACCTGG CTGGCCCAAA AGCAGAAGAG CAATGGCTGT 
TTCAGGAGTT CTGGGTCTCT GCTTAACAAT GCCCTCAAGG GTGGGGTGGA TGATGAAGTG 
ACCCTCTCTG CCTACATCAC CATCGCCCTT CTAGAGATGC CTCTCCCTGT CACACACCCT 
GTTGTCCGCA ATGCCCTGTT CTGCCTGGAC TCAGCTTGGA AGTCAGCCAA GGAAGGATCC 
CACGGCAGCC ACGTCTACAC CAAGGCACTG TTGGCCTATG CCTTTGCCCT GGCGGGTAAC 
CAGGAAAGGA GGACAGAGGT ACTGAGGTCA CTGCATGAGG AAGCCGTGAA GGAAGATAAT 
ACCATGCACT GGACACGACC TCAGAAACCC AGGGCTCTCA CGGAGGACAT TTACCAACCC 
AGGGCTCCGT CTGTGGAGGT GGAGATGACG GCCTACGTGC TCCTTGCTCA GGTCACGGCC 
CAGCCGGCCC CGACCCCAGA GGACCTCGAC CCTGCCACGA GAATCGTGAA GTGGATCTCA 
AAGCAGCAGA ATTGCCAGGG GGGCTTCTCC TCCACCCAGG ACACAGTGGT AGCTCTCCAC 
GCCTTGTCCA GATACGGAGC AGCCACTTTC ACCAGCGCTA CGAAGGTTGC ACAGGTGACC 
ATCCAGTCTT CAGGGACATT TTCCACAAAA TTCCAAGTGG AAAACAGCAA CCGCCTGTTA 
CTACAGCAGG TTTCACTGCC AGAAGTGCCT GGAGAGTACA GCATGTCAGT CACAGGAGAA 
GGATGTGTTT ACCTCCAGAC ATCTTTGAAA TACAATATTC TCCCGAAAAA AGACGAGTTC 
CCATTTGCTT TGGAGGTGCA TACTCTGCCC CAAACTTGTG ATGGACCCAA AGCCCACACC 
AGCTTCCAGA TCTCACTGAG TGTCAGTTAT ATTGGAAGCC GTCCAGCCTC CAATATGGCA 
ATTGTTGATG TGAAGATGGT ATCTGGCTTC ATTCCCTTGA AACCAACAGT GAAAATGCTA 
GAAAGATCTA ATGTGAGCCG AACAGAAGTT AGCAACAATC ATGTTTTGAT TTATCTGGAT 
AAGGTGACAG ATGAGACCCT GACCTTGACC TTCACAGTTC TGCAAGACAT CCCAGTAAGG 
GATCTGAAAC CGGCCATAGT GAAAGTCTAT GACTATTATG AGACGGATGA GTTTGCAGTT 
GCTGAGTACA GTGCTCCGTG CAGCAAAGGT AAGCAAACCT CCTTCCAGAT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102402539 ( XM_006069785.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006069847.1 Bubalus bubalis alpha-2-macroglobulin (A2M), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu24505
Clone ID Related Accession (Same CDS sequence)	XM_006069785.1
Accession Version	XM_006069785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	alpha-2-macroglobulin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785373.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGA ACAAACTCCT TTATCCAAGT CTTACTCTTC TTCTGCTCTT GCTGCCGACA 
GATGCTTCAG TCTCTGGAAA ACCGCAATAC ATGGTGCTGG TCCCCTCCCT GCTCCATACT 
GGGACCCCTG AGAAGGGCTG CCTCCTTCTG AGCCACCTGA ATGAGACGGT GACCGTCAGC 
GCCTCCCTGG AGTCCGTCAG AGAGAACAGG AGCCTCTTCA CTGACGTGGT GGCAGAGAAG 
GACTTATTCC ACTGTGTCTC CTTCACTCTC CCAAGATCTC CAACCAGGCA AGAGGTAATG 
TTCCTTACCA TCCAAGTAAA AGGACCAACC CAAGAATTCA AGAAGCGGAC CACAGTGTTG 
GTTAAAAACG AAGAGAGCCT GGTCTTTGTC CAGACAGACA AACCCATCTA TAAACCAGAG 
CAGACAGTGA ACTTTCGTAT TGTCTTGTTG GATGAAAGCT TTCACCCCCT GAATGAGTTG 
GTTCCACTAG TATATGTTGA GGACCCCAAA GGAAATCGCA TCGCACAGTG GCAGAACCTC 
GAGGTAGAGA ATGGCCTCCA GCAGCTGACC TTCCCCCTCT CATCAGAGCC CTTTCAGGGC 
TCCTATAAGG TGGTGGTACA GAAGGGATCA GGTGGGACTG CAGAGCACCC CTTCACCGTG 
GAGGAATTCG TGCTTCCTAA GTTTGAAGTA CAAGTAAGAA TGCCAAAGAT AATCACCATC 
TTGGAAGAAG AGGTGAAAGT GTCTGTGTGC GGCCTATACA CTTATGGGAA GCCTGTCCCA 
GGACGTGTGA CTATGAACAT GTGCAGAAAG TACAGGAACC CTTCTAATTG TTATGGTGAA 
GAATCCAATG CTGTCTGTGA GAAGTTCAGT GGACAGCTGA ACAATGAGGG CTGCTTCTCT 
CAGCAAGTAA ACACCAAGAT CTTCCAGATG AAGAGACAAG AGTTTGAAAT GAAAATCGAA 
GTGGAAGCCA AGATCCAAGA AGAAGGAACA GAGGTGGAAT TAACTGGAAA GGGGGCCACT 
GAAATAACAA CAACCATCAC CAAACTCTCA TTTGTCACAG TGGACTCAAA CCTTAGACGA 
GGAATTCCTT TCACGGGGCA GGTGCTCCTA GTGGACGGGA AAGGTGTCCC CATGCCAAAT 
AAAGTCATCT TCATCACAGC AAACGAAGCC AACCACAACT CTAACACCAC CACTGATGAG 
CACGGCCTGG CACAGTTCTC CATCACCACC ACCAACATCA AGGGCACCTC CCTCAGTATC 
AGGGTCAAAT ACAAGGATCA CAGCCCCTGT TACGGCTACC AGTGGCTGTC AGAAGACCAT 
CAAGACGCTT ATCACAGTGC TAATCTTGTG TTCTCCCAAA GCAACAGCTT TGTCTACCTG 
GAGCCTTTGC CTCGTAAACT GCCCTGTGGC AAAACTCAGA CAGTCCAGGC ACATTATGTG 
CTGAAGGGAG AGGTCCTAAA GGATCTAAAG GAGCTCGTGT TCTATTACCT GATAATGGCA 
AAAGGAGGCA TTGTCCGATC TGGGACTCAC ACACTGCCCG TGGAGCAGGG AGAGATGCAA 
GGCCACTTTT CCATACCAGT CCCTGTGGAG TCAGACATTG CTCCTGTCGC TAGGCTGCTC 
ATCTATGCCA TTTTACCTGA TGGAGAAGTG GTAGGGGATT CTGCAAGATA TGAAGTTGAA 
CAATGTCTGA GCAACAAGGT GGGTTTGAAC TTCAGCCCCG GACAAAGTTT CCCAGCCTCC 
CAGGCCCACC TTCGAGTCAC CGCTTCCCCT CAGTCCCTCT GCGCCCTCCG CGCCGTGGAC 
CAAAGTGTGC TGCTCATGAG GCCCGAAGCC GAGCTCTCAG CAGCCACGGT TTATAACCTG 
CTACCAGTCA AGGACCTCAG CAGTTTCCCC TCAAGTGTAA ATCAACAGGA GGAAGACAGT 
GAAGGCTGCA TCAGTCGTGA CAATGTCTAT ATCAATGGAA TCATGTATTT CCCAGTATCA 
AATACAAATG AAAAAGATAT GTATAGCTTC CTGCAGGATA TGGGCTTGAA GGCATTTACC 
AACTCAAAGA TTCATAAACC CAAGATATGT CCACAGCCTG AAGAACATAG AGTACAGCAT 
CACACACTAT TAGCTTCTCC AGTCAGAGCT GAAATGGGAA GAGACAGTAA TTTTGTGCGT 
TTTGACGATA CTTCAGAGCC TCCCACAGAG ACTGTACGAA AGTACTTCCC TGAGACATGG 
ATCTGGGACT TGGTGGTTGT AAGCTCTTCA GGTGTGCATG AAGTAGAAGT AACAGTCCCT 
GATACCATCA CTGAGTGGAA GGCAGGGGCC CTCTGCCTGT CCAGAGACAC TGGACTTGGT 
CTCTCTCCGA CCGCCTCCCT CCGAGTCTTC CAGCCCTTCT TTGTGGAGCT TACAATGCCC 
TACTCTGTGA TCCGTGGAGA GGCCTTCACA CTCAAGGCCA CAGTGTTAAA CTACCTTCCC 
AAATGCATCC GGGTCAGTGT GCATCTAGAA GCCTCTCCTG CATTCCTAGC TGTTCCAGAA 
AAGGAACAAG AGACTTACTG CATCTGTGAA AATGGCCGGC AGACTGTGTC CTGGGCGGTA 
ACTCCAAAGT CCTTAGGGAA TGTGAATTTC ACAGTGAGTG CAGAGGCAGT GGAGTCTCAA 
GAGCTGTGTG GGAGTGAAGT GCCTGTGGTC CCTGAGCATG GAAGGAAAGA CACAATCATC 
AAGCCCCTAT TGGTTGAACC TGAAGGACTA GAGAAGGAAG TAATATTCAA CTCCCTGCTT 
TGTCCATCAG GTGCCAAGGT GTCTGAATCA TTATCTCTGA AGCTGCCAGC AAATGTGGTG 
GAAGAATCTG CCCGAGCCTC CTTCTCAGTT TTAGGAGACA TATTAGGCTC TGCCATGAGA 
AACACCCAAA ATCTCCTCCA AATGCCCTAT GGTTGTGGAG AACAGAATAT GGCCCTCTTT 
GCCCCTAACA TCTATGTTCT GGATTATCTA AATGAGACAC AGCAGCTGAC TGCAGAGCTC 
AAGTCCAAGG CTATCCTCTA CCTCAACACT GGTTACCAGA GACAGCTACT CTACAAGCAC 
TTCGATGGCT CTTACAGCAC CTTTGGGGAG CATCATGGCA ACAGTGAGGG CAATACTTGG 
CTCACGGCCT TTGTACTGAA GAGTTTTGCC CAGGCTCGAG GCTATATTTT CATTGATGAA 
GCACATATTA CTGAAGCTCT CACCTGGCTG GCCCAAAAGC AGAAGAGCAA TGGCTGTTTC 
AGGAGTTCTG GGTCTCTGCT TAACAATGCC CTCAAGGGTG GGGTGGATGA TGAAGTGACC 
CTCTCTGCCT ACATCACCAT CGCCCTTCTA GAGATGCCTC TCCCTGTCAC ACACCCTGTT 
GTCCGCAATG CCCTGTTCTG CCTGGACTCA GCTTGGAAGT CAGCCAAGGA AGGATCCCAC 
GGCAGCCACG TCTACACCAA GGCACTGTTG GCCTATGCCT TTGCCCTGGC GGGTAACCAG 
GAAAGGAGGA CAGAGGTACT GAGGTCACTG CATGAGGAAG CCGTGAAGGA AGATAATACC 
ATGCACTGGA CACGACCTCA GAAACCCAGG GCTCTCACGG AGGACATTTA CCAACCCAGG 
GCTCCGTCTG TGGAGGTGGA GATGACGGCC TACGTGCTCC TTGCTCAGGT CACGGCCCAG 
CCGGCCCCGA CCCCAGAGGA CCTCGACCCT GCCACGAGAA TCGTGAAGTG GATCTCAAAG 
CAGCAGAATT GCCAGGGGGG CTTCTCCTCC ACCCAGGACA CAGTGGTAGC TCTCCACGCC 
TTGTCCAGAT ACGGAGCAGC CACTTTCACC AGCGCTACGA AGGTTGCACA GGTGACCATC 
CAGTCTTCAG GGACATTTTC CACAAAATTC CAAGTGGAAA ACAGCAACCG CCTGTTACTA 
CAGCAGGTTT CACTGCCAGA AGTGCCTGGA GAGTACAGCA TGTCAGTCAC AGGAGAAGGA 
TGTGTTTACC TCCAGACATC TTTGAAATAC AATATTCTCC CGAAAAAAGA CGAGTTCCCA 
TTTGCTTTGG AGGTGCATAC TCTGCCCCAA ACTTGTGATG GACCCAAAGC CCACACCAGC 
TTCCAGATCT CACTGAGTGT CAGTTATATT GGAAGCCGTC CAGCCTCCAA TATGGCAATT 
GTTGATGTGA AGATGGTATC TGGCTTCATT CCCTTGAAAC CAACAGTGAA AATGCTAGAA 
AGATCTAATG TGAGCCGAAC AGAAGTTAGC AACAATCATG TTTTGATTTA TCTGGATAAG 
GTGACAGATG AGACCCTGAC CTTGACCTTC ACAGTTCTGC AAGACATCCC AGTAAGGGAT 
CTGAAACCGG CCATAGTGAA AGTCTATGAC TATTATGAGA CGGATGAGTT TGCAGTTGCT 
GAGTACAGTG CTCCGTGCAG CAAAGGTAAG CAAACCTCCT TCCAGATGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006069847.1
CDS	311..4744
Translation	MGKNKLLYPSLTLLLLLLPTDASVSGKPQYMVLVPSLLHTGTPEKGCLLLSHLNETVTVSASLESVRENRSLFTDVVAEKDLFHCVSFTLPRSPTRQEVMFLTIQVKGPTQEFKKRTTVLVKNEESLVFVQTDKPIYKPEQTVNFRIVLLDESFHPLNELVPLVYVEDPKGNRIAQWQNLEVENGLQQLTFPLSSEPFQGSYKVVVQKGSGGTAEHPFTVEEFVLPKFEVQVRMPKIITILEEEVKVSVCGLYTYGKPVPGRVTMNMCRKYRNPSNCYGEESNAVCEKFSGQLNNEGCFSQQVNTKIFQMKRQEFEMKIEVEAKIQEEGTEVELTGKGATEITTTITKLSFVTVDSNLRRGIPFTGQVLLVDGKGVPMPNKVIFITANEANHNSNTTTDEHGLAQFSITTTNIKGTSLSIRVKYKDHSPCYGYQWLSEDHQDAYHSANLVFSQSNSFVYLEPLPRKLPCGKTQTVQAHYVLKGEVLKDLKELVFYYLIMAKGGIVRSGTHTLPVEQGEMQGHFSIPVPVESDIAPVARLLIYAILPDGEVVGDSARYEVEQCLSNKVGLNFSPGQSFPASQAHLRVTASPQSLCALRAVDQSVLLMRPEAELSAATVYNLLPVKDLSSFPSSVNQQEEDSEGCISRDNVYINGIMYFPVSNTNEKDMYSFLQDMGLKAFTNSKIHKPKICPQPEEHRVQHHTLLASPVRAEMGRDSNFVRFDDTSEPPTETVRKYFPETWIWDLVVVSSSGVHEVEVTVPDTITEWKAGALCLSRDTGLGLSPTASLRVFQPFFVELTMPYSVIRGEAFTLKATVLNYLPKCIRVSVHLEASPAFLAVPEKEQETYCICENGRQTVSWAVTPKSLGNVNFTVSAEAVESQELCGSEVPVVPEHGRKDTIIKPLLVEPEGLEKEVIFNSLLCPSGAKVSESLSLKLPANVVEESARASFSVLGDILGSAMRNTQNLLQMPYGCGEQNMALFAPNIYVLDYLNETQQLTAELKSKAILYLNTGYQRQLLYKHFDGSYSTFGEHHGNSEGNTWLTAFVLKSFAQARGYIFIDEAHITEALTWLAQKQKSNGCFRSSGSLLNNALKGGVDDEVTLSAYITIALLEMPLPVTHPVVRNALFCLDSAWKSAKEGSHGSHVYTKALLAYAFALAGNQERRTEVLRSLHEEAVKEDNTMHWTRPQKPRALTEDIYQPRAPSVEVEMTAYVLLAQVTAQPAPTPEDLDPATRIVKWISKQQNCQGGFSSTQDTVVALHALSRYGAATFTSATKVAQVTIQSSGTFSTKFQVENSNRLLLQQVSLPEVPGEYSMSVTGEGCVYLQTSLKYNILPKKDEFPFALEVHTLPQTCDGPKAHTSFQISLSVSYIGSRPASNMAIVDVKMVSGFIPLKPTVKMLERSNVSRTEVSNNHVLIYLDKVTDETLTLTFTVLQDIPVRDLKPAIVKVYDYYETDEFAVAEYSAPCSKGKQTSFQME

Target ORF information:

RefSeq Version	XM_006069785.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis alpha-2-macroglobulin (A2M), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006069785.1

ORF Insert Sequence:

ATGGGGAAGA ACAAACTCCT TTATCCAAGT CTTACTCTTC TTCTGCTCTT GCTGCCGACA 
GATGCTTCAG TCTCTGGAAA ACCGCAATAC ATGGTGCTGG TCCCCTCCCT GCTCCATACT 
GGGACCCCTG AGAAGGGCTG CCTCCTTCTG AGCCACCTGA ATGAGACGGT GACCGTCAGC 
GCCTCCCTGG AGTCCGTCAG AGAGAACAGG AGCCTCTTCA CTGACGTGGT GGCAGAGAAG 
GACTTATTCC ACTGTGTCTC CTTCACTCTC CCAAGATCTC CAACCAGGCA AGAGGTAATG 
TTCCTTACCA TCCAAGTAAA AGGACCAACC CAAGAATTCA AGAAGCGGAC CACAGTGTTG 
GTTAAAAACG AAGAGAGCCT GGTCTTTGTC CAGACAGACA AACCCATCTA TAAACCAGAG 
CAGACAGTGA ACTTTCGTAT TGTCTTGTTG GATGAAAGCT TTCACCCCCT GAATGAGTTG 
GTTCCACTAG TATATGTTGA GGACCCCAAA GGAAATCGCA TCGCACAGTG GCAGAACCTC 
GAGGTAGAGA ATGGCCTCCA GCAGCTGACC TTCCCCCTCT CATCAGAGCC CTTTCAGGGC 
TCCTATAAGG TGGTGGTACA GAAGGGATCA GGTGGGACTG CAGAGCACCC CTTCACCGTG 
GAGGAATTCG TGCTTCCTAA GTTTGAAGTA CAAGTAAGAA TGCCAAAGAT AATCACCATC 
TTGGAAGAAG AGGTGAAAGT GTCTGTGTGC GGCCTATACA CTTATGGGAA GCCTGTCCCA 
GGACGTGTGA CTATGAACAT GTGCAGAAAG TACAGGAACC CTTCTAATTG TTATGGTGAA 
GAATCCAATG CTGTCTGTGA GAAGTTCAGT GGACAGCTGA ACAATGAGGG CTGCTTCTCT 
CAGCAAGTAA ACACCAAGAT CTTCCAGATG AAGAGACAAG AGTTTGAAAT GAAAATCGAA 
GTGGAAGCCA AGATCCAAGA AGAAGGAACA GAGGTGGAAT TAACTGGAAA GGGGGCCACT 
GAAATAACAA CAACCATCAC CAAACTCTCA TTTGTCACAG TGGACTCAAA CCTTAGACGA 
GGAATTCCTT TCACGGGGCA GGTGCTCCTA GTGGACGGGA AAGGTGTCCC CATGCCAAAT 
AAAGTCATCT TCATCACAGC AAACGAAGCC AACCACAACT CTAACACCAC CACTGATGAG 
CACGGCCTGG CACAGTTCTC CATCACCACC ACCAACATCA AGGGCACCTC CCTCAGTATC 
AGGGTCAAAT ACAAGGATCA CAGCCCCTGT TACGGCTACC AGTGGCTGTC AGAAGACCAT 
CAAGACGCTT ATCACAGTGC TAATCTTGTG TTCTCCCAAA GCAACAGCTT TGTCTACCTG 
GAGCCTTTGC CTCGTAAACT GCCCTGTGGC AAAACTCAGA CAGTCCAGGC ACATTATGTG 
CTGAAGGGAG AGGTCCTAAA GGATCTAAAG GAGCTCGTGT TCTATTACCT GATAATGGCA 
AAAGGAGGCA TTGTCCGATC TGGGACTCAC ACACTGCCCG TGGAGCAGGG AGAGATGCAA 
GGCCACTTTT CCATACCAGT CCCTGTGGAG TCAGACATTG CTCCTGTCGC TAGGCTGCTC 
ATCTATGCCA TTTTACCTGA TGGAGAAGTG GTAGGGGATT CTGCAAGATA TGAAGTTGAA 
CAATGTCTGA GCAACAAGGT GGGTTTGAAC TTCAGCCCCG GACAAAGTTT CCCAGCCTCC 
CAGGCCCACC TTCGAGTCAC CGCTTCCCCT CAGTCCCTCT GCGCCCTCCG CGCCGTGGAC 
CAAAGTGTGC TGCTCATGAG GCCCGAAGCC GAGCTCTCAG CAGCCACGGT TTATAACCTG 
CTACCAGTCA AGGACCTCAG CAGTTTCCCC TCAAGTGTAA ATCAACAGGA GGAAGACAGT 
GAAGGCTGCA TCAGTCGTGA CAATGTCTAT ATCAATGGAA TCATGTATTT CCCAGTATCA 
AATACAAATG AAAAAGATAT GTATAGCTTC CTGCAGGATA TGGGCTTGAA GGCATTTACC 
AACTCAAAGA TTCATAAACC CAAGATATGT CCACAGCCTG AAGAACATAG AGTACAGCAT 
CACACACTAT TAGCTTCTCC AGTCAGAGCT GAAATGGGAA GAGACAGTAA TTTTGTGCGT 
TTTGACGATA CTTCAGAGCC TCCCACAGAG ACTGTACGAA AGTACTTCCC TGAGACATGG 
ATCTGGGACT TGGTGGTTGT AAGCTCTTCA GGTGTGCATG AAGTAGAAGT AACAGTCCCT 
GATACCATCA CTGAGTGGAA GGCAGGGGCC CTCTGCCTGT CCAGAGACAC TGGACTTGGT 
CTCTCTCCGA CCGCCTCCCT CCGAGTCTTC CAGCCCTTCT TTGTGGAGCT TACAATGCCC 
TACTCTGTGA TCCGTGGAGA GGCCTTCACA CTCAAGGCCA CAGTGTTAAA CTACCTTCCC 
AAATGCATCC GGGTCAGTGT GCATCTAGAA GCCTCTCCTG CATTCCTAGC TGTTCCAGAA 
AAGGAACAAG AGACTTACTG CATCTGTGAA AATGGCCGGC AGACTGTGTC CTGGGCGGTA 
ACTCCAAAGT CCTTAGGGAA TGTGAATTTC ACAGTGAGTG CAGAGGCAGT GGAGTCTCAA 
GAGCTGTGTG GGAGTGAAGT GCCTGTGGTC CCTGAGCATG GAAGGAAAGA CACAATCATC 
AAGCCCCTAT TGGTTGAACC TGAAGGACTA GAGAAGGAAG TAATATTCAA CTCCCTGCTT 
TGTCCATCAG GTGCCAAGGT GTCTGAATCA TTATCTCTGA AGCTGCCAGC AAATGTGGTG 
GAAGAATCTG CCCGAGCCTC CTTCTCAGTT TTAGGAGACA TATTAGGCTC TGCCATGAGA 
AACACCCAAA ATCTCCTCCA AATGCCCTAT GGTTGTGGAG AACAGAATAT GGCCCTCTTT 
GCCCCTAACA TCTATGTTCT GGATTATCTA AATGAGACAC AGCAGCTGAC TGCAGAGCTC 
AAGTCCAAGG CTATCCTCTA CCTCAACACT GGTTACCAGA GACAGCTACT CTACAAGCAC 
TTCGATGGCT CTTACAGCAC CTTTGGGGAG CATCATGGCA ACAGTGAGGG CAATACTTGG 
CTCACGGCCT TTGTACTGAA GAGTTTTGCC CAGGCTCGAG GCTATATTTT CATTGATGAA 
GCACATATTA CTGAAGCTCT CACCTGGCTG GCCCAAAAGC AGAAGAGCAA TGGCTGTTTC 
AGGAGTTCTG GGTCTCTGCT TAACAATGCC CTCAAGGGTG GGGTGGATGA TGAAGTGACC 
CTCTCTGCCT ACATCACCAT CGCCCTTCTA GAGATGCCTC TCCCTGTCAC ACACCCTGTT 
GTCCGCAATG CCCTGTTCTG CCTGGACTCA GCTTGGAAGT CAGCCAAGGA AGGATCCCAC 
GGCAGCCACG TCTACACCAA GGCACTGTTG GCCTATGCCT TTGCCCTGGC GGGTAACCAG 
GAAAGGAGGA CAGAGGTACT GAGGTCACTG CATGAGGAAG CCGTGAAGGA AGATAATACC 
ATGCACTGGA CACGACCTCA GAAACCCAGG GCTCTCACGG AGGACATTTA CCAACCCAGG 
GCTCCGTCTG TGGAGGTGGA GATGACGGCC TACGTGCTCC TTGCTCAGGT CACGGCCCAG 
CCGGCCCCGA CCCCAGAGGA CCTCGACCCT GCCACGAGAA TCGTGAAGTG GATCTCAAAG 
CAGCAGAATT GCCAGGGGGG CTTCTCCTCC ACCCAGGACA CAGTGGTAGC TCTCCACGCC 
TTGTCCAGAT ACGGAGCAGC CACTTTCACC AGCGCTACGA AGGTTGCACA GGTGACCATC 
CAGTCTTCAG GGACATTTTC CACAAAATTC CAAGTGGAAA ACAGCAACCG CCTGTTACTA 
CAGCAGGTTT CACTGCCAGA AGTGCCTGGA GAGTACAGCA TGTCAGTCAC AGGAGAAGGA 
TGTGTTTACC TCCAGACATC TTTGAAATAC AATATTCTCC CGAAAAAAGA CGAGTTCCCA 
TTTGCTTTGG AGGTGCATAC TCTGCCCCAA ACTTGTGATG GACCCAAAGC CCACACCAGC 
TTCCAGATCT CACTGAGTGT CAGTTATATT GGAAGCCGTC CAGCCTCCAA TATGGCAATT 
GTTGATGTGA AGATGGTATC TGGCTTCATT CCCTTGAAAC CAACAGTGAA AATGCTAGAA 
AGATCTAATG TGAGCCGAAC AGAAGTTAGC AACAATCATG TTTTGATTTA TCTGGATAAG 
GTGACAGATG AGACCCTGAC CTTGACCTTC ACAGTTCTGC AAGACATCCC AGTAAGGGAT 
CTGAAACCGG CCATAGTGAA AGTCTATGAC TATTATGAGA CGGATGAGTT TGCAGTTGCT 
GAGTACAGTG CTCCGTGCAG CAAAGGTAAG CAAACCTCCT TCCAGATGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102402539 ( XM_006069786.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006069848.1 Bubalus bubalis alpha-2-macroglobulin (A2M), transcript variant X3, mRNA.

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CloneID	OBu24506
Clone ID Related Accession (Same CDS sequence)	XM_006069786.1 , XM_006069786.2
Accession Version	XM_006069786.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	alpha-2-macroglobulin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785373.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAG GCAGACTTCT TCACTCATGT CTCCTGCTAC TTCTTGTCAT TCGGTTTGCT 
ACCAGCACCT CAAATTCTGT GGAACCGCAA TACATGGTGC TGGTCCCCTC CCTGCTCCAT 
ACTGGGACCC CTGAGAAGGG CTGCCTCCTT CTGAGCCACC TGAATGAGAC GGTGACCGTC 
AGCGCCTCCC TGGAGTCCGT CAGAGAGAAC AGGAGCCTCT TCACTGACGT GGTGGCAGAG 
AAGGACTTAT TCCACTGTGT CTCCTTCACT CTCCCAAGAT CTCCAACCAG GCAAGAGGTA 
ATGTTCCTTA CCATCCAAGT AAAAGGACCA ACCCAAGAAT TCAAGAAGCG GACCACAGTG 
TTGGTTAAAA ACGAAGAGAG CCTGGTCTTT GTCCAGACAG ACAAACCCAT CTATAAACCA 
GAGCAGACAG TGAACTTTCG TATTGTCTTG TTGGATGAAA GCTTTCACCC CCTGAATGAG 
TTGGTTCCAC TAGTATATGT TGAGGACCCC AAAGGAAATC GCATCGCACA GTGGCAGAAC 
CTCGAGGTAG AGAATGGCCT CCAGCAGCTG ACCTTCCCCC TCTCATCAGA GCCCTTTCAG 
GGCTCCTATA AGGTGGTGGT ACAGAAGGGA TCAGGTGGGA CTGCAGAGCA CCCCTTCACC 
GTGGAGGAAT TCGTGCTTCC TAAGTTTGAA GTACAAGTAA GAATGCCAAA GATAATCACC 
ATCTTGGAAG AAGAGGTGAA AGTGTCTGTG TGCGGCCTAT ACACTTATGG GAAGCCTGTC 
CCAGGACGTG TGACTATGAA CATGTGCAGA AAGTACAGGA ACCCTTCTAA TTGTTATGGT 
GAAGAATCCA ATGCTGTCTG TGAGAAGTTC AGTGGACAGC TGAACAATGA GGGCTGCTTC 
TCTCAGCAAG TAAACACCAA GATCTTCCAG ATGAAGAGAC AAGAGTTTGA AATGAAAATC 
GAAGTGGAAG CCAAGATCCA AGAAGAAGGA ACAGAGGTGG AATTAACTGG AAAGGGGGCC 
ACTGAAATAA CAACAACCAT CACCAAACTC TCATTTGTCA CAGTGGACTC AAACCTTAGA 
CGAGGAATTC CTTTCACGGG GCAGGTGCTC CTAGTGGACG GGAAAGGTGT CCCCATGCCA 
AATAAAGTCA TCTTCATCAC AGCAAACGAA GCCAACCACA ACTCTAACAC CACCACTGAT 
GAGCACGGCC TGGCACAGTT CTCCATCACC ACCACCAACA TCAAGGGCAC CTCCCTCAGT 
ATCAGGGTCA AATACAAGGA TCACAGCCCC TGTTACGGCT ACCAGTGGCT GTCAGAAGAC 
CATCAAGACG CTTATCACAG TGCTAATCTT GTGTTCTCCC AAAGCAACAG CTTTGTCTAC 
CTGGAGCCTT TGCCTCGTAA ACTGCCCTGT GGCAAAACTC AGACAGTCCA GGCACATTAT 
GTGCTGAAGG GAGAGGTCCT AAAGGATCTA AAGGAGCTCG TGTTCTATTA CCTGATAATG 
GCAAAAGGAG GCATTGTCCG ATCTGGGACT CACACACTGC CCGTGGAGCA GGGAGAGATG 
CAAGGCCACT TTTCCATACC AGTCCCTGTG GAGTCAGACA TTGCTCCTGT CGCTAGGCTG 
CTCATCTATG CCATTTTACC TGATGGAGAA GTGGTAGGGG ATTCTGCAAG ATATGAAGTT 
GAACAATGTC TGAGCAACAA GGTGGGTTTG AACTTCAGCC CCGGACAAAG TTTCCCAGCC 
TCCCAGGCCC ACCTTCGAGT CACCGCTTCC CCTCAGTCCC TCTGCGCCCT CCGCGCCGTG 
GACCAAAGTG TGCTGCTCAT GAGGCCCGAA GCCGAGCTCT CAGCAGCCAC GGTTTATAAC 
CTGCTACCAG TCAAGGACCT CAGCAGTTTC CCCTCAAGTG TAAATCAACA GGAGGAAGAC 
AGTGAAGGCT GCATCAGTCG TGACAATGTC TATATCAATG GAATCATGTA TTTCCCAGTA 
TCAAATACAA ATGAAAAAGA TATGTATAGC TTCCTGCAGG ATATGGGCTT GAAGGCATTT 
ACCAACTCAA AGATTCATAA ACCCAAGATA TGTCCACAGC CTGAAGAACA TAGAGTACAG 
CATCACACAC TATTAGCTTC TCCAGTCAGA GCTGAAATGG GAAGAGACAG TAATTTTGTG 
CGTTTTGACG ATACTTCAGA GCCTCCCACA GAGACTGTAC GAAAGTACTT CCCTGAGACA 
TGGATCTGGG ACTTGGTGGT TGTAAGCTCT TCAGGTGTGC ATGAAGTAGA AGTAACAGTC 
CCTGATACCA TCACTGAGTG GAAGGCAGGG GCCCTCTGCC TGTCCAGAGA CACTGGACTT 
GGTCTCTCTC CGACCGCCTC CCTCCGAGTC TTCCAGCCCT TCTTTGTGGA GCTTACAATG 
CCCTACTCTG TGATCCGTGG AGAGGCCTTC ACACTCAAGG CCACAGTGTT AAACTACCTT 
CCCAAATGCA TCCGGGTCAG TGTGCATCTA GAAGCCTCTC CTGCATTCCT AGCTGTTCCA 
GAAAAGGAAC AAGAGACTTA CTGCATCTGT GAAAATGGCC GGCAGACTGT GTCCTGGGCG 
GTAACTCCAA AGTCCTTAGG GAATGTGAAT TTCACAGTGA GTGCAGAGGC AGTGGAGTCT 
CAAGAGCTGT GTGGGAGTGA AGTGCCTGTG GTCCCTGAGC ATGGAAGGAA AGACACAATC 
ATCAAGCCCC TATTGGTTGA ACCTGAAGGA CTAGAGAAGG AAGTAATATT CAACTCCCTG 
CTTTGTCCAT CAGGTGCCAA GGTGTCTGAA TCATTATCTC TGAAGCTGCC AGCAAATGTG 
GTGGAAGAAT CTGCCCGAGC CTCCTTCTCA GTTTTAGGAG ACATATTAGG CTCTGCCATG 
AGAAACACCC AAAATCTCCT CCAAATGCCC TATGGTTGTG GAGAACAGAA TATGGCCCTC 
TTTGCCCCTA ACATCTATGT TCTGGATTAT CTAAATGAGA CACAGCAGCT GACTGCAGAG 
CTCAAGTCCA AGGCTATCCT CTACCTCAAC ACTGGTTACC AGAGACAGCT ACTCTACAAG 
CACTTCGATG GCTCTTACAG CACCTTTGGG GAGCATCATG GCAACAGTGA GGGCAATACT 
TGGCTCACGG CCTTTGTACT GAAGAGTTTT GCCCAGGCTC GAGGCTATAT TTTCATTGAT 
GAAGCACATA TTACTGAAGC TCTCACCTGG CTGGCCCAAA AGCAGAAGAG CAATGGCTGT 
TTCAGGAGTT CTGGGTCTCT GCTTAACAAT GCCCTCAAGG GTGGGGTGGA TGATGAAGTG 
ACCCTCTCTG CCTACATCAC CATCGCCCTT CTAGAGATGC CTCTCCCTGT CACACACCCT 
GTTGTCCGCA ATGCCCTGTT CTGCCTGGAC TCAGCTTGGA AGTCAGCCAA GGAAGGATCC 
CACGGCAGCC ACGTCTACAC CAAGGCACTG TTGGCCTATG CCTTTGCCCT GGCGGGTAAC 
CAGGAAAGGA GGACAGAGGT ACTGAGGTCA CTGCATGAGG AAGCCGTGAA GGAAGATAAT 
ACCATGCACT GGACACGACC TCAGAAACCC AGGGCTCTCA CGGAGGACAT TTACCAACCC 
AGGGCTCCGT CTGTGGAGGT GGAGATGACG GCCTACGTGC TCCTTGCTCA GGTCACGGCC 
CAGCCGGCCC CGACCCCAGA GGACCTCGAC CCTGCCACGA GAATCGTGAA GTGGATCTCA 
AAGCAGCAGA ATTGCCAGGG GGGCTTCTCC TCCACCCAGG ACACAGTGGT AGCTCTCCAC 
GCCTTGTCCA GATACGGAGC AGCCACTTTC ACCAGCGCTA CGAAGGTTGC ACAGGTGACC 
ATCCAGTCTT CAGGGACATT TTCCACAAAA TTCCAAGTGG AAAACAGCAA CCGCCTGTTA 
CTACAGCAGG TTTCACTGCC AGAAGTGCCT GGAGAGTACA GCATGTCAGT CACAGGAGAA 
GGATGTGTTT ACCTCCAGAC ATCTTTGAAA TACAATATTC TCCCGAAAAA AGACGAGTTC 
CCATTTGCTT TGGAGGTGCA TACTCTGCCC CAAACTTGTG ATGGACCCAA AGCCCACACC 
AGCTTCCAGA TCTCACTGAG TGTCAGTTAT ATTGGAAGCC GTCCAGCCTC CAATATGGCA 
ATTGTTGATG TGAAGATGGT ATCTGGCTTC ATTCCCTTGA AACCAACAGT GAAAATGCTA 
GAAAGATCTA ATGTGAGCCG AACAGAAGTT AGCAACAATC ATGTTTTGAT TTATCTGGAT 
AAGGTGACAG ATGAGACCCT GACCTTGACC TTCACAGTTC TGCAAGACAT CCCAGTAAGG 
GATCTGAAAC CGGCCATAGT GAAAGTCTAT GACTATTATG AGACGGATGA GTTTGCAGTT 
GCTGAGTACA GTGCTCCGTG CAGCAAAGAT ACTGGAAATG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006069848.1
CDS	60..4484
Translation	MGEGRLLHSCLLLLLVIRFATSTSNSVEPQYMVLVPSLLHTGTPEKGCLLLSHLNETVTVSASLESVRENRSLFTDVVAEKDLFHCVSFTLPRSPTRQEVMFLTIQVKGPTQEFKKRTTVLVKNEESLVFVQTDKPIYKPEQTVNFRIVLLDESFHPLNELVPLVYVEDPKGNRIAQWQNLEVENGLQQLTFPLSSEPFQGSYKVVVQKGSGGTAEHPFTVEEFVLPKFEVQVRMPKIITILEEEVKVSVCGLYTYGKPVPGRVTMNMCRKYRNPSNCYGEESNAVCEKFSGQLNNEGCFSQQVNTKIFQMKRQEFEMKIEVEAKIQEEGTEVELTGKGATEITTTITKLSFVTVDSNLRRGIPFTGQVLLVDGKGVPMPNKVIFITANEANHNSNTTTDEHGLAQFSITTTNIKGTSLSIRVKYKDHSPCYGYQWLSEDHQDAYHSANLVFSQSNSFVYLEPLPRKLPCGKTQTVQAHYVLKGEVLKDLKELVFYYLIMAKGGIVRSGTHTLPVEQGEMQGHFSIPVPVESDIAPVARLLIYAILPDGEVVGDSARYEVEQCLSNKVGLNFSPGQSFPASQAHLRVTASPQSLCALRAVDQSVLLMRPEAELSAATVYNLLPVKDLSSFPSSVNQQEEDSEGCISRDNVYINGIMYFPVSNTNEKDMYSFLQDMGLKAFTNSKIHKPKICPQPEEHRVQHHTLLASPVRAEMGRDSNFVRFDDTSEPPTETVRKYFPETWIWDLVVVSSSGVHEVEVTVPDTITEWKAGALCLSRDTGLGLSPTASLRVFQPFFVELTMPYSVIRGEAFTLKATVLNYLPKCIRVSVHLEASPAFLAVPEKEQETYCICENGRQTVSWAVTPKSLGNVNFTVSAEAVESQELCGSEVPVVPEHGRKDTIIKPLLVEPEGLEKEVIFNSLLCPSGAKVSESLSLKLPANVVEESARASFSVLGDILGSAMRNTQNLLQMPYGCGEQNMALFAPNIYVLDYLNETQQLTAELKSKAILYLNTGYQRQLLYKHFDGSYSTFGEHHGNSEGNTWLTAFVLKSFAQARGYIFIDEAHITEALTWLAQKQKSNGCFRSSGSLLNNALKGGVDDEVTLSAYITIALLEMPLPVTHPVVRNALFCLDSAWKSAKEGSHGSHVYTKALLAYAFALAGNQERRTEVLRSLHEEAVKEDNTMHWTRPQKPRALTEDIYQPRAPSVEVEMTAYVLLAQVTAQPAPTPEDLDPATRIVKWISKQQNCQGGFSSTQDTVVALHALSRYGAATFTSATKVAQVTIQSSGTFSTKFQVENSNRLLLQQVSLPEVPGEYSMSVTGEGCVYLQTSLKYNILPKKDEFPFALEVHTLPQTCDGPKAHTSFQISLSVSYIGSRPASNMAIVDVKMVSGFIPLKPTVKMLERSNVSRTEVSNNHVLIYLDKVTDETLTLTFTVLQDIPVRDLKPAIVKVYDYYETDEFAVAEYSAPCSKDTGNA

Target ORF information:

RefSeq Version	XM_006069786.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis alpha-2-macroglobulin (A2M), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006069786.1

ORF Insert Sequence:

ATGGGGGAAG GCAGACTTCT TCACTCATGT CTCCTGCTAC TTCTTGTCAT TCGGTTTGCT 
ACCAGCACCT CAAATTCTGT GGAACCGCAA TACATGGTGC TGGTCCCCTC CCTGCTCCAT 
ACTGGGACCC CTGAGAAGGG CTGCCTCCTT CTGAGCCACC TGAATGAGAC GGTGACCGTC 
AGCGCCTCCC TGGAGTCCGT CAGAGAGAAC AGGAGCCTCT TCACTGACGT GGTGGCAGAG 
AAGGACTTAT TCCACTGTGT CTCCTTCACT CTCCCAAGAT CTCCAACCAG GCAAGAGGTA 
ATGTTCCTTA CCATCCAAGT AAAAGGACCA ACCCAAGAAT TCAAGAAGCG GACCACAGTG 
TTGGTTAAAA ACGAAGAGAG CCTGGTCTTT GTCCAGACAG ACAAACCCAT CTATAAACCA 
GAGCAGACAG TGAACTTTCG TATTGTCTTG TTGGATGAAA GCTTTCACCC CCTGAATGAG 
TTGGTTCCAC TAGTATATGT TGAGGACCCC AAAGGAAATC GCATCGCACA GTGGCAGAAC 
CTCGAGGTAG AGAATGGCCT CCAGCAGCTG ACCTTCCCCC TCTCATCAGA GCCCTTTCAG 
GGCTCCTATA AGGTGGTGGT ACAGAAGGGA TCAGGTGGGA CTGCAGAGCA CCCCTTCACC 
GTGGAGGAAT TCGTGCTTCC TAAGTTTGAA GTACAAGTAA GAATGCCAAA GATAATCACC 
ATCTTGGAAG AAGAGGTGAA AGTGTCTGTG TGCGGCCTAT ACACTTATGG GAAGCCTGTC 
CCAGGACGTG TGACTATGAA CATGTGCAGA AAGTACAGGA ACCCTTCTAA TTGTTATGGT 
GAAGAATCCA ATGCTGTCTG TGAGAAGTTC AGTGGACAGC TGAACAATGA GGGCTGCTTC 
TCTCAGCAAG TAAACACCAA GATCTTCCAG ATGAAGAGAC AAGAGTTTGA AATGAAAATC 
GAAGTGGAAG CCAAGATCCA AGAAGAAGGA ACAGAGGTGG AATTAACTGG AAAGGGGGCC 
ACTGAAATAA CAACAACCAT CACCAAACTC TCATTTGTCA CAGTGGACTC AAACCTTAGA 
CGAGGAATTC CTTTCACGGG GCAGGTGCTC CTAGTGGACG GGAAAGGTGT CCCCATGCCA 
AATAAAGTCA TCTTCATCAC AGCAAACGAA GCCAACCACA ACTCTAACAC CACCACTGAT 
GAGCACGGCC TGGCACAGTT CTCCATCACC ACCACCAACA TCAAGGGCAC CTCCCTCAGT 
ATCAGGGTCA AATACAAGGA TCACAGCCCC TGTTACGGCT ACCAGTGGCT GTCAGAAGAC 
CATCAAGACG CTTATCACAG TGCTAATCTT GTGTTCTCCC AAAGCAACAG CTTTGTCTAC 
CTGGAGCCTT TGCCTCGTAA ACTGCCCTGT GGCAAAACTC AGACAGTCCA GGCACATTAT 
GTGCTGAAGG GAGAGGTCCT AAAGGATCTA AAGGAGCTCG TGTTCTATTA CCTGATAATG 
GCAAAAGGAG GCATTGTCCG ATCTGGGACT CACACACTGC CCGTGGAGCA GGGAGAGATG 
CAAGGCCACT TTTCCATACC AGTCCCTGTG GAGTCAGACA TTGCTCCTGT CGCTAGGCTG 
CTCATCTATG CCATTTTACC TGATGGAGAA GTGGTAGGGG ATTCTGCAAG ATATGAAGTT 
GAACAATGTC TGAGCAACAA GGTGGGTTTG AACTTCAGCC CCGGACAAAG TTTCCCAGCC 
TCCCAGGCCC ACCTTCGAGT CACCGCTTCC CCTCAGTCCC TCTGCGCCCT CCGCGCCGTG 
GACCAAAGTG TGCTGCTCAT GAGGCCCGAA GCCGAGCTCT CAGCAGCCAC GGTTTATAAC 
CTGCTACCAG TCAAGGACCT CAGCAGTTTC CCCTCAAGTG TAAATCAACA GGAGGAAGAC 
AGTGAAGGCT GCATCAGTCG TGACAATGTC TATATCAATG GAATCATGTA TTTCCCAGTA 
TCAAATACAA ATGAAAAAGA TATGTATAGC TTCCTGCAGG ATATGGGCTT GAAGGCATTT 
ACCAACTCAA AGATTCATAA ACCCAAGATA TGTCCACAGC CTGAAGAACA TAGAGTACAG 
CATCACACAC TATTAGCTTC TCCAGTCAGA GCTGAAATGG GAAGAGACAG TAATTTTGTG 
CGTTTTGACG ATACTTCAGA GCCTCCCACA GAGACTGTAC GAAAGTACTT CCCTGAGACA 
TGGATCTGGG ACTTGGTGGT TGTAAGCTCT TCAGGTGTGC ATGAAGTAGA AGTAACAGTC 
CCTGATACCA TCACTGAGTG GAAGGCAGGG GCCCTCTGCC TGTCCAGAGA CACTGGACTT 
GGTCTCTCTC CGACCGCCTC CCTCCGAGTC TTCCAGCCCT TCTTTGTGGA GCTTACAATG 
CCCTACTCTG TGATCCGTGG AGAGGCCTTC ACACTCAAGG CCACAGTGTT AAACTACCTT 
CCCAAATGCA TCCGGGTCAG TGTGCATCTA GAAGCCTCTC CTGCATTCCT AGCTGTTCCA 
GAAAAGGAAC AAGAGACTTA CTGCATCTGT GAAAATGGCC GGCAGACTGT GTCCTGGGCG 
GTAACTCCAA AGTCCTTAGG GAATGTGAAT TTCACAGTGA GTGCAGAGGC AGTGGAGTCT 
CAAGAGCTGT GTGGGAGTGA AGTGCCTGTG GTCCCTGAGC ATGGAAGGAA AGACACAATC 
ATCAAGCCCC TATTGGTTGA ACCTGAAGGA CTAGAGAAGG AAGTAATATT CAACTCCCTG 
CTTTGTCCAT CAGGTGCCAA GGTGTCTGAA TCATTATCTC TGAAGCTGCC AGCAAATGTG 
GTGGAAGAAT CTGCCCGAGC CTCCTTCTCA GTTTTAGGAG ACATATTAGG CTCTGCCATG 
AGAAACACCC AAAATCTCCT CCAAATGCCC TATGGTTGTG GAGAACAGAA TATGGCCCTC 
TTTGCCCCTA ACATCTATGT TCTGGATTAT CTAAATGAGA CACAGCAGCT GACTGCAGAG 
CTCAAGTCCA AGGCTATCCT CTACCTCAAC ACTGGTTACC AGAGACAGCT ACTCTACAAG 
CACTTCGATG GCTCTTACAG CACCTTTGGG GAGCATCATG GCAACAGTGA GGGCAATACT 
TGGCTCACGG CCTTTGTACT GAAGAGTTTT GCCCAGGCTC GAGGCTATAT TTTCATTGAT 
GAAGCACATA TTACTGAAGC TCTCACCTGG CTGGCCCAAA AGCAGAAGAG CAATGGCTGT 
TTCAGGAGTT CTGGGTCTCT GCTTAACAAT GCCCTCAAGG GTGGGGTGGA TGATGAAGTG 
ACCCTCTCTG CCTACATCAC CATCGCCCTT CTAGAGATGC CTCTCCCTGT CACACACCCT 
GTTGTCCGCA ATGCCCTGTT CTGCCTGGAC TCAGCTTGGA AGTCAGCCAA GGAAGGATCC 
CACGGCAGCC ACGTCTACAC CAAGGCACTG TTGGCCTATG CCTTTGCCCT GGCGGGTAAC 
CAGGAAAGGA GGACAGAGGT ACTGAGGTCA CTGCATGAGG AAGCCGTGAA GGAAGATAAT 
ACCATGCACT GGACACGACC TCAGAAACCC AGGGCTCTCA CGGAGGACAT TTACCAACCC 
AGGGCTCCGT CTGTGGAGGT GGAGATGACG GCCTACGTGC TCCTTGCTCA GGTCACGGCC 
CAGCCGGCCC CGACCCCAGA GGACCTCGAC CCTGCCACGA GAATCGTGAA GTGGATCTCA 
AAGCAGCAGA ATTGCCAGGG GGGCTTCTCC TCCACCCAGG ACACAGTGGT AGCTCTCCAC 
GCCTTGTCCA GATACGGAGC AGCCACTTTC ACCAGCGCTA CGAAGGTTGC ACAGGTGACC 
ATCCAGTCTT CAGGGACATT TTCCACAAAA TTCCAAGTGG AAAACAGCAA CCGCCTGTTA 
CTACAGCAGG TTTCACTGCC AGAAGTGCCT GGAGAGTACA GCATGTCAGT CACAGGAGAA 
GGATGTGTTT ACCTCCAGAC ATCTTTGAAA TACAATATTC TCCCGAAAAA AGACGAGTTC 
CCATTTGCTT TGGAGGTGCA TACTCTGCCC CAAACTTGTG ATGGACCCAA AGCCCACACC 
AGCTTCCAGA TCTCACTGAG TGTCAGTTAT ATTGGAAGCC GTCCAGCCTC CAATATGGCA 
ATTGTTGATG TGAAGATGGT ATCTGGCTTC ATTCCCTTGA AACCAACAGT GAAAATGCTA 
GAAAGATCTA ATGTGAGCCG AACAGAAGTT AGCAACAATC ATGTTTTGAT TTATCTGGAT 
AAGGTGACAG ATGAGACCCT GACCTTGACC TTCACAGTTC TGCAAGACAT CCCAGTAAGG 
GATCTGAAAC CGGCCATAGT GAAAGTCTAT GACTATTATG AGACGGATGA GTTTGCAGTT 
GCTGAGTACA GTGCTCCGTG CAGCAAAGAT ACTGGAAATG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102402539 ( XM_006069786.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006069848.1 Bubalus bubalis alpha-2-macroglobulin (LOC102402539), transcript variant X1, mRNA.

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CloneID	OBu24506
Clone ID Related Accession (Same CDS sequence)	XM_006069786.1 , XM_006069786.2
Accession Version	XM_006069786.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	alpha-2-macroglobulin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006069786.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAG GCAGACTTCT TCACTCATGT CTCCTGCTAC TTCTTGTCAT TCGGTTTGCT 
ACCAGCACCT CAAATTCTGT GGAACCGCAA TACATGGTGC TGGTCCCCTC CCTGCTCCAT 
ACTGGGACCC CTGAGAAGGG CTGCCTCCTT CTGAGCCACC TGAATGAGAC GGTGACCGTC 
AGCGCCTCCC TGGAGTCCGT CAGAGAGAAC AGGAGCCTCT TCACTGACGT GGTGGCAGAG 
AAGGACTTAT TCCACTGTGT CTCCTTCACT CTCCCAAGAT CTCCAACCAG GCAAGAGGTA 
ATGTTCCTTA CCATCCAAGT AAAAGGACCA ACCCAAGAAT TCAAGAAGCG GACCACAGTG 
TTGGTTAAAA ACGAAGAGAG CCTGGTCTTT GTCCAGACAG ACAAACCCAT CTATAAACCA 
GAGCAGACAG TGAACTTTCG TATTGTCTTG TTGGATGAAA GCTTTCACCC CCTGAATGAG 
TTGGTTCCAC TAGTATATGT TGAGGACCCC AAAGGAAATC GCATCGCACA GTGGCAGAAC 
CTCGAGGTAG AGAATGGCCT CCAGCAGCTG ACCTTCCCCC TCTCATCAGA GCCCTTTCAG 
GGCTCCTATA AGGTGGTGGT ACAGAAGGGA TCAGGTGGGA CTGCAGAGCA CCCCTTCACC 
GTGGAGGAAT TCGTGCTTCC TAAGTTTGAA GTACAAGTAA GAATGCCAAA GATAATCACC 
ATCTTGGAAG AAGAGGTGAA AGTGTCTGTG TGCGGCCTAT ACACTTATGG GAAGCCTGTC 
CCAGGACGTG TGACTATGAA CATGTGCAGA AAGTACAGGA ACCCTTCTAA TTGTTATGGT 
GAAGAATCCA ATGCTGTCTG TGAGAAGTTC AGTGGACAGC TGAACAATGA GGGCTGCTTC 
TCTCAGCAAG TAAACACCAA GATCTTCCAG ATGAAGAGAC AAGAGTTTGA AATGAAAATC 
GAAGTGGAAG CCAAGATCCA AGAAGAAGGA ACAGAGGTGG AATTAACTGG AAAGGGGGCC 
ACTGAAATAA CAACAACCAT CACCAAACTC TCATTTGTCA CAGTGGACTC AAACCTTAGA 
CGAGGAATTC CTTTCACGGG GCAGGTGCTC CTAGTGGACG GGAAAGGTGT CCCCATGCCA 
AATAAAGTCA TCTTCATCAC AGCAAACGAA GCCAACCACA ACTCTAACAC CACCACTGAT 
GAGCACGGCC TGGCACAGTT CTCCATCACC ACCACCAACA TCAAGGGCAC CTCCCTCAGT 
ATCAGGGTCA AATACAAGGA TCACAGCCCC TGTTACGGCT ACCAGTGGCT GTCAGAAGAC 
CATCAAGACG CTTATCACAG TGCTAATCTT GTGTTCTCCC AAAGCAACAG CTTTGTCTAC 
CTGGAGCCTT TGCCTCGTAA ACTGCCCTGT GGCAAAACTC AGACAGTCCA GGCACATTAT 
GTGCTGAAGG GAGAGGTCCT AAAGGATCTA AAGGAGCTCG TGTTCTATTA CCTGATAATG 
GCAAAAGGAG GCATTGTCCG ATCTGGGACT CACACACTGC CCGTGGAGCA GGGAGAGATG 
CAAGGCCACT TTTCCATACC AGTCCCTGTG GAGTCAGACA TTGCTCCTGT CGCTAGGCTG 
CTCATCTATG CCATTTTACC TGATGGAGAA GTGGTAGGGG ATTCTGCAAG ATATGAAGTT 
GAACAATGTC TGAGCAACAA GGTGGGTTTG AACTTCAGCC CCGGACAAAG TTTCCCAGCC 
TCCCAGGCCC ACCTTCGAGT CACCGCTTCC CCTCAGTCCC TCTGCGCCCT CCGCGCCGTG 
GACCAAAGTG TGCTGCTCAT GAGGCCCGAA GCCGAGCTCT CAGCAGCCAC GGTTTATAAC 
CTGCTACCAG TCAAGGACCT CAGCAGTTTC CCCTCAAGTG TAAATCAACA GGAGGAAGAC 
AGTGAAGGCT GCATCAGTCG TGACAATGTC TATATCAATG GAATCATGTA TTTCCCAGTA 
TCAAATACAA ATGAAAAAGA TATGTATAGC TTCCTGCAGG ATATGGGCTT GAAGGCATTT 
ACCAACTCAA AGATTCATAA ACCCAAGATA TGTCCACAGC CTGAAGAACA TAGAGTACAG 
CATCACACAC TATTAGCTTC TCCAGTCAGA GCTGAAATGG GAAGAGACAG TAATTTTGTG 
CGTTTTGACG ATACTTCAGA GCCTCCCACA GAGACTGTAC GAAAGTACTT CCCTGAGACA 
TGGATCTGGG ACTTGGTGGT TGTAAGCTCT TCAGGTGTGC ATGAAGTAGA AGTAACAGTC 
CCTGATACCA TCACTGAGTG GAAGGCAGGG GCCCTCTGCC TGTCCAGAGA CACTGGACTT 
GGTCTCTCTC CGACCGCCTC CCTCCGAGTC TTCCAGCCCT TCTTTGTGGA GCTTACAATG 
CCCTACTCTG TGATCCGTGG AGAGGCCTTC ACACTCAAGG CCACAGTGTT AAACTACCTT 
CCCAAATGCA TCCGGGTCAG TGTGCATCTA GAAGCCTCTC CTGCATTCCT AGCTGTTCCA 
GAAAAGGAAC AAGAGACTTA CTGCATCTGT GAAAATGGCC GGCAGACTGT GTCCTGGGCG 
GTAACTCCAA AGTCCTTAGG GAATGTGAAT TTCACAGTGA GTGCAGAGGC AGTGGAGTCT 
CAAGAGCTGT GTGGGAGTGA AGTGCCTGTG GTCCCTGAGC ATGGAAGGAA AGACACAATC 
ATCAAGCCCC TATTGGTTGA ACCTGAAGGA CTAGAGAAGG AAGTAATATT CAACTCCCTG 
CTTTGTCCAT CAGGTGCCAA GGTGTCTGAA TCATTATCTC TGAAGCTGCC AGCAAATGTG 
GTGGAAGAAT CTGCCCGAGC CTCCTTCTCA GTTTTAGGAG ACATATTAGG CTCTGCCATG 
AGAAACACCC AAAATCTCCT CCAAATGCCC TATGGTTGTG GAGAACAGAA TATGGCCCTC 
TTTGCCCCTA ACATCTATGT TCTGGATTAT CTAAATGAGA CACAGCAGCT GACTGCAGAG 
CTCAAGTCCA AGGCTATCCT CTACCTCAAC ACTGGTTACC AGAGACAGCT ACTCTACAAG 
CACTTCGATG GCTCTTACAG CACCTTTGGG GAGCATCATG GCAACAGTGA GGGCAATACT 
TGGCTCACGG CCTTTGTACT GAAGAGTTTT GCCCAGGCTC GAGGCTATAT TTTCATTGAT 
GAAGCACATA TTACTGAAGC TCTCACCTGG CTGGCCCAAA AGCAGAAGAG CAATGGCTGT 
TTCAGGAGTT CTGGGTCTCT GCTTAACAAT GCCCTCAAGG GTGGGGTGGA TGATGAAGTG 
ACCCTCTCTG CCTACATCAC CATCGCCCTT CTAGAGATGC CTCTCCCTGT CACACACCCT 
GTTGTCCGCA ATGCCCTGTT CTGCCTGGAC TCAGCTTGGA AGTCAGCCAA GGAAGGATCC 
CACGGCAGCC ACGTCTACAC CAAGGCACTG TTGGCCTATG CCTTTGCCCT GGCGGGTAAC 
CAGGAAAGGA GGACAGAGGT ACTGAGGTCA CTGCATGAGG AAGCCGTGAA GGAAGATAAT 
ACCATGCACT GGACACGACC TCAGAAACCC AGGGCTCTCA CGGAGGACAT TTACCAACCC 
AGGGCTCCGT CTGTGGAGGT GGAGATGACG GCCTACGTGC TCCTTGCTCA GGTCACGGCC 
CAGCCGGCCC CGACCCCAGA GGACCTCGAC CCTGCCACGA GAATCGTGAA GTGGATCTCA 
AAGCAGCAGA ATTGCCAGGG GGGCTTCTCC TCCACCCAGG ACACAGTGGT AGCTCTCCAC 
GCCTTGTCCA GATACGGAGC AGCCACTTTC ACCAGCGCTA CGAAGGTTGC ACAGGTGACC 
ATCCAGTCTT CAGGGACATT TTCCACAAAA TTCCAAGTGG AAAACAGCAA CCGCCTGTTA 
CTACAGCAGG TTTCACTGCC AGAAGTGCCT GGAGAGTACA GCATGTCAGT CACAGGAGAA 
GGATGTGTTT ACCTCCAGAC ATCTTTGAAA TACAATATTC TCCCGAAAAA AGACGAGTTC 
CCATTTGCTT TGGAGGTGCA TACTCTGCCC CAAACTTGTG ATGGACCCAA AGCCCACACC 
AGCTTCCAGA TCTCACTGAG TGTCAGTTAT ATTGGAAGCC GTCCAGCCTC CAATATGGCA 
ATTGTTGATG TGAAGATGGT ATCTGGCTTC ATTCCCTTGA AACCAACAGT GAAAATGCTA 
GAAAGATCTA ATGTGAGCCG AACAGAAGTT AGCAACAATC ATGTTTTGAT TTATCTGGAT 
AAGGTGACAG ATGAGACCCT GACCTTGACC TTCACAGTTC TGCAAGACAT CCCAGTAAGG 
GATCTGAAAC CGGCCATAGT GAAAGTCTAT GACTATTATG AGACGGATGA GTTTGCAGTT 
GCTGAGTACA GTGCTCCGTG CAGCAAAGAT ACTGGAAATG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006069848.1
CDS	63..4487
Translation	MGEGRLLHSCLLLLLVIRFATSTSNSVEPQYMVLVPSLLHTGTPEKGCLLLSHLNETVTVSASLESVRENRSLFTDVVAEKDLFHCVSFTLPRSPTRQEVMFLTIQVKGPTQEFKKRTTVLVKNEESLVFVQTDKPIYKPEQTVNFRIVLLDESFHPLNELVPLVYVEDPKGNRIAQWQNLEVENGLQQLTFPLSSEPFQGSYKVVVQKGSGGTAEHPFTVEEFVLPKFEVQVRMPKIITILEEEVKVSVCGLYTYGKPVPGRVTMNMCRKYRNPSNCYGEESNAVCEKFSGQLNNEGCFSQQVNTKIFQMKRQEFEMKIEVEAKIQEEGTEVELTGKGATEITTTITKLSFVTVDSNLRRGIPFTGQVLLVDGKGVPMPNKVIFITANEANHNSNTTTDEHGLAQFSITTTNIKGTSLSIRVKYKDHSPCYGYQWLSEDHQDAYHSANLVFSQSNSFVYLEPLPRKLPCGKTQTVQAHYVLKGEVLKDLKELVFYYLIMAKGGIVRSGTHTLPVEQGEMQGHFSIPVPVESDIAPVARLLIYAILPDGEVVGDSARYEVEQCLSNKVGLNFSPGQSFPASQAHLRVTASPQSLCALRAVDQSVLLMRPEAELSAATVYNLLPVKDLSSFPSSVNQQEEDSEGCISRDNVYINGIMYFPVSNTNEKDMYSFLQDMGLKAFTNSKIHKPKICPQPEEHRVQHHTLLASPVRAEMGRDSNFVRFDDTSEPPTETVRKYFPETWIWDLVVVSSSGVHEVEVTVPDTITEWKAGALCLSRDTGLGLSPTASLRVFQPFFVELTMPYSVIRGEAFTLKATVLNYLPKCIRVSVHLEASPAFLAVPEKEQETYCICENGRQTVSWAVTPKSLGNVNFTVSAEAVESQELCGSEVPVVPEHGRKDTIIKPLLVEPEGLEKEVIFNSLLCPSGAKVSESLSLKLPANVVEESARASFSVLGDILGSAMRNTQNLLQMPYGCGEQNMALFAPNIYVLDYLNETQQLTAELKSKAILYLNTGYQRQLLYKHFDGSYSTFGEHHGNSEGNTWLTAFVLKSFAQARGYIFIDEAHITEALTWLAQKQKSNGCFRSSGSLLNNALKGGVDDEVTLSAYITIALLEMPLPVTHPVVRNALFCLDSAWKSAKEGSHGSHVYTKALLAYAFALAGNQERRTEVLRSLHEEAVKEDNTMHWTRPQKPRALTEDIYQPRAPSVEVEMTAYVLLAQVTAQPAPTPEDLDPATRIVKWISKQQNCQGGFSSTQDTVVALHALSRYGAATFTSATKVAQVTIQSSGTFSTKFQVENSNRLLLQQVSLPEVPGEYSMSVTGEGCVYLQTSLKYNILPKKDEFPFALEVHTLPQTCDGPKAHTSFQISLSVSYIGSRPASNMAIVDVKMVSGFIPLKPTVKMLERSNVSRTEVSNNHVLIYLDKVTDETLTLTFTVLQDIPVRDLKPAIVKVYDYYETDEFAVAEYSAPCSKDTGNA

Target ORF information:

RefSeq Version	XM_006069786.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis alpha-2-macroglobulin (LOC102402539), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006069786.2

ORF Insert Sequence:

ATGGGGGAAG GCAGACTTCT TCACTCATGT CTCCTGCTAC TTCTTGTCAT TCGGTTTGCT 
ACCAGCACCT CAAATTCTGT GGAACCGCAA TACATGGTGC TGGTCCCCTC CCTGCTCCAT 
ACTGGGACCC CTGAGAAGGG CTGCCTCCTT CTGAGCCACC TGAATGAGAC GGTGACCGTC 
AGCGCCTCCC TGGAGTCCGT CAGAGAGAAC AGGAGCCTCT TCACTGACGT GGTGGCAGAG 
AAGGACTTAT TCCACTGTGT CTCCTTCACT CTCCCAAGAT CTCCAACCAG GCAAGAGGTA 
ATGTTCCTTA CCATCCAAGT AAAAGGACCA ACCCAAGAAT TCAAGAAGCG GACCACAGTG 
TTGGTTAAAA ACGAAGAGAG CCTGGTCTTT GTCCAGACAG ACAAACCCAT CTATAAACCA 
GAGCAGACAG TGAACTTTCG TATTGTCTTG TTGGATGAAA GCTTTCACCC CCTGAATGAG 
TTGGTTCCAC TAGTATATGT TGAGGACCCC AAAGGAAATC GCATCGCACA GTGGCAGAAC 
CTCGAGGTAG AGAATGGCCT CCAGCAGCTG ACCTTCCCCC TCTCATCAGA GCCCTTTCAG 
GGCTCCTATA AGGTGGTGGT ACAGAAGGGA TCAGGTGGGA CTGCAGAGCA CCCCTTCACC 
GTGGAGGAAT TCGTGCTTCC TAAGTTTGAA GTACAAGTAA GAATGCCAAA GATAATCACC 
ATCTTGGAAG AAGAGGTGAA AGTGTCTGTG TGCGGCCTAT ACACTTATGG GAAGCCTGTC 
CCAGGACGTG TGACTATGAA CATGTGCAGA AAGTACAGGA ACCCTTCTAA TTGTTATGGT 
GAAGAATCCA ATGCTGTCTG TGAGAAGTTC AGTGGACAGC TGAACAATGA GGGCTGCTTC 
TCTCAGCAAG TAAACACCAA GATCTTCCAG ATGAAGAGAC AAGAGTTTGA AATGAAAATC 
GAAGTGGAAG CCAAGATCCA AGAAGAAGGA ACAGAGGTGG AATTAACTGG AAAGGGGGCC 
ACTGAAATAA CAACAACCAT CACCAAACTC TCATTTGTCA CAGTGGACTC AAACCTTAGA 
CGAGGAATTC CTTTCACGGG GCAGGTGCTC CTAGTGGACG GGAAAGGTGT CCCCATGCCA 
AATAAAGTCA TCTTCATCAC AGCAAACGAA GCCAACCACA ACTCTAACAC CACCACTGAT 
GAGCACGGCC TGGCACAGTT CTCCATCACC ACCACCAACA TCAAGGGCAC CTCCCTCAGT 
ATCAGGGTCA AATACAAGGA TCACAGCCCC TGTTACGGCT ACCAGTGGCT GTCAGAAGAC 
CATCAAGACG CTTATCACAG TGCTAATCTT GTGTTCTCCC AAAGCAACAG CTTTGTCTAC 
CTGGAGCCTT TGCCTCGTAA ACTGCCCTGT GGCAAAACTC AGACAGTCCA GGCACATTAT 
GTGCTGAAGG GAGAGGTCCT AAAGGATCTA AAGGAGCTCG TGTTCTATTA CCTGATAATG 
GCAAAAGGAG GCATTGTCCG ATCTGGGACT CACACACTGC CCGTGGAGCA GGGAGAGATG 
CAAGGCCACT TTTCCATACC AGTCCCTGTG GAGTCAGACA TTGCTCCTGT CGCTAGGCTG 
CTCATCTATG CCATTTTACC TGATGGAGAA GTGGTAGGGG ATTCTGCAAG ATATGAAGTT 
GAACAATGTC TGAGCAACAA GGTGGGTTTG AACTTCAGCC CCGGACAAAG TTTCCCAGCC 
TCCCAGGCCC ACCTTCGAGT CACCGCTTCC CCTCAGTCCC TCTGCGCCCT CCGCGCCGTG 
GACCAAAGTG TGCTGCTCAT GAGGCCCGAA GCCGAGCTCT CAGCAGCCAC GGTTTATAAC 
CTGCTACCAG TCAAGGACCT CAGCAGTTTC CCCTCAAGTG TAAATCAACA GGAGGAAGAC 
AGTGAAGGCT GCATCAGTCG TGACAATGTC TATATCAATG GAATCATGTA TTTCCCAGTA 
TCAAATACAA ATGAAAAAGA TATGTATAGC TTCCTGCAGG ATATGGGCTT GAAGGCATTT 
ACCAACTCAA AGATTCATAA ACCCAAGATA TGTCCACAGC CTGAAGAACA TAGAGTACAG 
CATCACACAC TATTAGCTTC TCCAGTCAGA GCTGAAATGG GAAGAGACAG TAATTTTGTG 
CGTTTTGACG ATACTTCAGA GCCTCCCACA GAGACTGTAC GAAAGTACTT CCCTGAGACA 
TGGATCTGGG ACTTGGTGGT TGTAAGCTCT TCAGGTGTGC ATGAAGTAGA AGTAACAGTC 
CCTGATACCA TCACTGAGTG GAAGGCAGGG GCCCTCTGCC TGTCCAGAGA CACTGGACTT 
GGTCTCTCTC CGACCGCCTC CCTCCGAGTC TTCCAGCCCT TCTTTGTGGA GCTTACAATG 
CCCTACTCTG TGATCCGTGG AGAGGCCTTC ACACTCAAGG CCACAGTGTT AAACTACCTT 
CCCAAATGCA TCCGGGTCAG TGTGCATCTA GAAGCCTCTC CTGCATTCCT AGCTGTTCCA 
GAAAAGGAAC AAGAGACTTA CTGCATCTGT GAAAATGGCC GGCAGACTGT GTCCTGGGCG 
GTAACTCCAA AGTCCTTAGG GAATGTGAAT TTCACAGTGA GTGCAGAGGC AGTGGAGTCT 
CAAGAGCTGT GTGGGAGTGA AGTGCCTGTG GTCCCTGAGC ATGGAAGGAA AGACACAATC 
ATCAAGCCCC TATTGGTTGA ACCTGAAGGA CTAGAGAAGG AAGTAATATT CAACTCCCTG 
CTTTGTCCAT CAGGTGCCAA GGTGTCTGAA TCATTATCTC TGAAGCTGCC AGCAAATGTG 
GTGGAAGAAT CTGCCCGAGC CTCCTTCTCA GTTTTAGGAG ACATATTAGG CTCTGCCATG 
AGAAACACCC AAAATCTCCT CCAAATGCCC TATGGTTGTG GAGAACAGAA TATGGCCCTC 
TTTGCCCCTA ACATCTATGT TCTGGATTAT CTAAATGAGA CACAGCAGCT GACTGCAGAG 
CTCAAGTCCA AGGCTATCCT CTACCTCAAC ACTGGTTACC AGAGACAGCT ACTCTACAAG 
CACTTCGATG GCTCTTACAG CACCTTTGGG GAGCATCATG GCAACAGTGA GGGCAATACT 
TGGCTCACGG CCTTTGTACT GAAGAGTTTT GCCCAGGCTC GAGGCTATAT TTTCATTGAT 
GAAGCACATA TTACTGAAGC TCTCACCTGG CTGGCCCAAA AGCAGAAGAG CAATGGCTGT 
TTCAGGAGTT CTGGGTCTCT GCTTAACAAT GCCCTCAAGG GTGGGGTGGA TGATGAAGTG 
ACCCTCTCTG CCTACATCAC CATCGCCCTT CTAGAGATGC CTCTCCCTGT CACACACCCT 
GTTGTCCGCA ATGCCCTGTT CTGCCTGGAC TCAGCTTGGA AGTCAGCCAA GGAAGGATCC 
CACGGCAGCC ACGTCTACAC CAAGGCACTG TTGGCCTATG CCTTTGCCCT GGCGGGTAAC 
CAGGAAAGGA GGACAGAGGT ACTGAGGTCA CTGCATGAGG AAGCCGTGAA GGAAGATAAT 
ACCATGCACT GGACACGACC TCAGAAACCC AGGGCTCTCA CGGAGGACAT TTACCAACCC 
AGGGCTCCGT CTGTGGAGGT GGAGATGACG GCCTACGTGC TCCTTGCTCA GGTCACGGCC 
CAGCCGGCCC CGACCCCAGA GGACCTCGAC CCTGCCACGA GAATCGTGAA GTGGATCTCA 
AAGCAGCAGA ATTGCCAGGG GGGCTTCTCC TCCACCCAGG ACACAGTGGT AGCTCTCCAC 
GCCTTGTCCA GATACGGAGC AGCCACTTTC ACCAGCGCTA CGAAGGTTGC ACAGGTGACC 
ATCCAGTCTT CAGGGACATT TTCCACAAAA TTCCAAGTGG AAAACAGCAA CCGCCTGTTA 
CTACAGCAGG TTTCACTGCC AGAAGTGCCT GGAGAGTACA GCATGTCAGT CACAGGAGAA 
GGATGTGTTT ACCTCCAGAC ATCTTTGAAA TACAATATTC TCCCGAAAAA AGACGAGTTC 
CCATTTGCTT TGGAGGTGCA TACTCTGCCC CAAACTTGTG ATGGACCCAA AGCCCACACC 
AGCTTCCAGA TCTCACTGAG TGTCAGTTAT ATTGGAAGCC GTCCAGCCTC CAATATGGCA 
ATTGTTGATG TGAAGATGGT ATCTGGCTTC ATTCCCTTGA AACCAACAGT GAAAATGCTA 
GAAAGATCTA ATGTGAGCCG AACAGAAGTT AGCAACAATC ATGTTTTGAT TTATCTGGAT 
AAGGTGACAG ATGAGACCCT GACCTTGACC TTCACAGTTC TGCAAGACAT CCCAGTAAGG 
GATCTGAAAC CGGCCATAGT GAAAGTCTAT GACTATTATG AGACGGATGA GTTTGCAGTT 
GCTGAGTACA GTGCTCCGTG CAGCAAAGAT ACTGGAAATG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102402539 ( XM_025283184.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025138969.1 Bubalus bubalis alpha-2-macroglobulin (LOC102402539), transcript variant X2, mRNA.

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CloneID	OBu64113
Clone ID Related Accession (Same CDS sequence)	XM_025283184.1
Accession Version	XM_025283184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	alpha-2-macroglobulin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGA ACAAACTCCT TTATCCAAGT CTTACTCTTC TTCTGCTCTT GCTGCCGACA 
GATGCTTCAG TCTCTGGAAA ACCGCAATAC ATGGTGCTGG TCCCCTCCCT GCTCCATACT 
GGGACCCCTG AGAAGGGCTG CCTCCTTCTG AGCCACCTGA ATGAGACGGT GACCGTCAGC 
GCCTCCCTGG AGTCCGTCAG AGAGAACAGG AGCCTCTTCA CTGACGTGGT GGCAGAGAAG 
GACTTATTCC ACTGTGTCTC CTTCACTCTC CCAAGATCTC CAACCAGGCA AGAGGTAATG 
TTCCTTACCA TCCAAGTAAA AGGACCAACC CAAGAATTCA AGAAGCGGAC CACAGTGTTG 
GTTAAAAACG AAGAGAGCCT GGTCTTTGTC CAGACAGACA AACCCATCTA TAAACCAGAG 
CAGACAGTGA ACTTTCGTAT TGTCTTGTTG GATGAAAGCT TTCACCCCCT GAATGAGTTG 
GTTCCACTAG TATATGTTGA GGACCCCAAA GGAAATCGCA TCGCACAGTG GCAGAACCTC 
GAGGTAGAGA ATGGCCTCCA GCAGCTGACC TTCCCCCTCT CATCAGAGCC CTTTCAGGGC 
TCCTATAAGG TGGTGGTACA GAAGGGATCA GGTGGGACTG CAGAGCACCC CTTCACCGTG 
GAGGAATTCG TGCTTCCTAA GTTTGAAGTA CAAGTAAGAA TGCCAAAGAT AATCACCATC 
TTGGAAGAAG AGGTGAAAGT GTCTGTGTGC GGCCTATACA CTTATGGGAA GCCTGTCCCA 
GGACGTGTGA CTATGAACAT GTGCAGAAAG TACAGGAACC CTTCTAATTG TTATGGTGAA 
GAATCCAATG CTGTCTGTGA GAAGTTCAGT GGACAGCTGA ACAATGAGGG CTGCTTCTCT 
CAGCAAGTAA ACACCAAGAT CTTCCAGATG AAGAGACAAG AGTTTGAAAT GAAAATCGAA 
GTGGAAGCCA AGATCCAAGA AGAAGGAACA GAGGTGGAAT TAACTGGAAA GGGGGCCACT 
GAAATAACAA CAACCATCAC CAAACTCTCA TTTGTCACAG TGGACTCAAA CCTTAGACGA 
GGAATTCCTT TCACGGGGCA GGTGCTCCTA GTGGACGGGA AAGGTGTCCC CATGCCAAAT 
AAAGTCATCT TCATCACAGC AAACGAAGCC AACCACAACT CTAACACCAC CACTGATGAG 
CACGGCCTGG CACAGTTCTC CATCACCACC ACCAACATCA AGGGCACCTC CCTCAGTATC 
AGGGTCAAAT ACAAGGATCA CAGCCCCTGT TACGGCTACC AGTGGCTGTC AGAAGACCAT 
CAAGACGCTT ATCACAGTGC TAATCTTGTG TTCTCCCAAA GCAACAGCTT TGTCTACCTG 
GAGCCTTTGC CTCGTAAACT GCCCTGTGGC AAAACTCAGA CAGTCCAGGC ACATTATGTG 
CTGAAGGGAG AGGTCCTAAA GGATCTAAAG GAGCTCGTGT TCTATTACCT GATAATGGCA 
AAAGGAGGCA TTGTCCGATC TGGGACTCAC ACACTGCCCG TGGAGCAGGG AGAGATGCAA 
GGCCACTTTT CCATACCAGT CCCTGTGGAG TCAGACATTG CTCCTGTCGC TAGGCTGCTC 
ATCTATGCCA TTTTACCTGA TGGAGAAGTG GTAGGGGATT CTGCAAGATA TGAAGTTGAA 
CAATGTCTGA GCAACAAGGT GGGTTTGAAC TTCAGCCCCG GACAAAGTTT CCCAGCCTCC 
CAGGCCCACC TTCGAGTCAC CGCTTCCCCT CAGTCCCTCT GCGCCCTCCG CGCCGTGGAC 
CAAAGTGTGC TGCTCATGAG GCCCGAAGCC GAGCTCTCAG CAGCCACGGT TTATAACCTG 
CTACCAGTCA AGGACCTCAG CAGTTTCCCC TCAAGTGTAA ATCAACAGGA GGAAGACAGT 
GAAGGCTGCA TCAGTCGTGA CAATGTCTAT ATCAATGGAA TCATGTATTT CCCAGTATCA 
AATACAAATG AAAAAGATAT GTATAGCTTC CTGCAGGATA TGGGCTTGAA GGCATTTACC 
AACTCAAAGA TTCATAAACC CAAGATATGT CCACAGCCTG AAGAACATAG AGTACAGCAT 
CACACACTAT TAGCTTCTCC AGTCAGAGCT GAAATGGGAA GAGACAGTAA TTTTGTGCGT 
TTTGACGATA CTTCAGAGCC TCCCACAGAG ACTGTACGAA AGTACTTCCC TGAGACATGG 
ATCTGGGACT TGGTGGTTGT AAGCTCTTCA GGTGTGCATG AAGTAGAAGT AACAGTCCCT 
GATACCATCA CTGAGTGGAA GGCAGGGGCC CTCTGCCTGT CCAGAGACAC TGGACTTGGT 
CTCTCTCCGA CCGCCTCCCT CCGAGTCTTC CAGCCCTTCT TTGTGGAGCT TACAATGCCC 
TACTCTGTGA TCCGTGGAGA GGCCTTCACA CTCAAGGCCA CAGTGTTAAA CTACCTTCCC 
AAATGCATCC GGGTCAGTGT GCATCTAGAA GCCTCTCCTG CATTCCTAGC TGTTCCAGAA 
AAGGAACAAG AGACTTACTG CATCTGTGAA AATGGCCGGC AGACTGTGTC CTGGGCGGTA 
ACTCCAAAGT CCTTAGGGAA TGTGAATTTC ACAGTGAGTG CAGAGGCAGT GGAGTCTCAA 
GAGCTGTGTG GGAGTGAAGT GCCTGTGGTC CCTGAGCATG GAAGGAAAGA CACAATCATC 
AAGCCCCTAT TGGTTGAACC TGAAGGACTA GAGAAGGAAG TAATATTCAA CTCCCTGCTT 
TGTCCATCAG GTGCCAAGGT GTCTGAATCA TTATCTCTGA AGCTGCCAGC AAATGTGGTG 
GAAGAATCTG CCCGAGCCTC CTTCTCAGTT TTAGGAGACA TATTAGGCTC TGCCATGAGA 
AACACCCAAA ATCTCCTCCA AATGCCCTAT GGTTGTGGAG AACAGAATAT GGCCCTCTTT 
GCCCCTAACA TCTATGTTCT GGATTATCTA AATGAGACAC AGCAGCTGAC TGCAGAGCTC 
AAGTCCAAGG CTATCCTCTA CCTCAACACT GGTTACCAGA GACAGCTACT CTACAAGCAC 
TTCGATGGCT CTTACAGCAC CTTTGGGGAG CATCATGGCA ACAGTGAGGG CAATACTTGG 
CTCACGGCCT TTGTACTGAA GAGTTTTGCC CAGGCTCGAG GCTATATTTT CATTGATGAA 
GCACATATTA CTGAAGCTCT CACCTGGCTG GCCCAAAAGC AGAAGAGCAA TGGCTGTTTC 
AGGAGTTCTG GGTCTCTGCT TAACAATGCC CTCAAGGGTG GGGTGGATGA TGAAGTGACC 
CTCTCTGCCT ACATCACCAT CGCCCTTCTA GAGATGCCTC TCCCTGTCAC ACACCCTGTT 
GTCCGCAATG CCCTGTTCTG CCTGGACTCA GCTTGGAAGT CAGCCAAGGA AGGATCCCAC 
GGCAGCCACG TCTACACCAA GGCACTGTTG GCCTATGCCT TTGCCCTGGC GGGTAACCAG 
GAAAGGAGGA CAGAGGTACT GAGGTCACTG CATGAGGAAG CCGTGAAGGA AGATAATACC 
ATGCACTGGA CACGACCTCA GAAACCCAGG GCTCTCACGG AGGACATTTA CCAACCCAGG 
GCTCCGTCTG TGGAGGTGGA GATGACGGCC TACGTGCTCC TTGCTCAGGT CACGGCCCAG 
CCGGCCCCGA CCCCAGAGGA CCTCGACCCT GCCACGAGAA TCGTGAAGTG GATCTCAAAG 
CAGCAGAATT GCCAGGGGGG CTTCTCCTCC ACCCAGGACA CAGTGGTAGC TCTCCACGCC 
TTGTCCAGAT ACGGAGCAGC CACTTTCACC AGCGCTACGA AGGTTGCACA GGTGACCATC 
CAGTCTTCAG GGACATTTTC CACAAAATTC CAAGTGGAAA ACAGCAACCG CCTGTTACTA 
CAGCAGGTTT CACTGCCAGA AGTGCCTGGA GAGTACAGCA TGTCAGTCAC AGGAGAAGGA 
TGTGTTTACC TCCAGACATC TTTGAAATAC AATATTCTCC CGAAAAAAGA CGAGTTCCCA 
TTTGCTTTGG AGGTGCATAC TCTGCCCCAA ACTTGTGATG GACCCAAAGC CCACACCAGC 
TTCCAGATCT CACTGAGTGT CAGTTATATT GGAAGCCGTC CAGCCTCCAA TATGGCAATT 
GTTGATGTGA AGATGGTATC TGGCTTCATT CCCTTGAAAC CAACAGTGAA AATGCTAGAA 
AGATCTAATG TGAGCCGAAC AGAAGTTAGC AACAATCATG TTTTGATTTA TCTGGATAAG 
GTGACAGATG AGACCCTGAC CTTGACCTTC ACAGTTCTGC AAGACATCCC AGTAAGGGAT 
CTGAAACCGG CCATAGTGAA AGTCTATGAC TATTATGAGA CGGATGAGTT TGCAGTTGCT 
GAGTACAGTG CTCCGTGCAG CAAAGATACT GGAAATGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025138969.1
CDS	252..4673
Translation	MGKNKLLYPSLTLLLLLLPTDASVSGKPQYMVLVPSLLHTGTPEKGCLLLSHLNETVTVSASLESVRENRSLFTDVVAEKDLFHCVSFTLPRSPTRQEVMFLTIQVKGPTQEFKKRTTVLVKNEESLVFVQTDKPIYKPEQTVNFRIVLLDESFHPLNELVPLVYVEDPKGNRIAQWQNLEVENGLQQLTFPLSSEPFQGSYKVVVQKGSGGTAEHPFTVEEFVLPKFEVQVRMPKIITILEEEVKVSVCGLYTYGKPVPGRVTMNMCRKYRNPSNCYGEESNAVCEKFSGQLNNEGCFSQQVNTKIFQMKRQEFEMKIEVEAKIQEEGTEVELTGKGATEITTTITKLSFVTVDSNLRRGIPFTGQVLLVDGKGVPMPNKVIFITANEANHNSNTTTDEHGLAQFSITTTNIKGTSLSIRVKYKDHSPCYGYQWLSEDHQDAYHSANLVFSQSNSFVYLEPLPRKLPCGKTQTVQAHYVLKGEVLKDLKELVFYYLIMAKGGIVRSGTHTLPVEQGEMQGHFSIPVPVESDIAPVARLLIYAILPDGEVVGDSARYEVEQCLSNKVGLNFSPGQSFPASQAHLRVTASPQSLCALRAVDQSVLLMRPEAELSAATVYNLLPVKDLSSFPSSVNQQEEDSEGCISRDNVYINGIMYFPVSNTNEKDMYSFLQDMGLKAFTNSKIHKPKICPQPEEHRVQHHTLLASPVRAEMGRDSNFVRFDDTSEPPTETVRKYFPETWIWDLVVVSSSGVHEVEVTVPDTITEWKAGALCLSRDTGLGLSPTASLRVFQPFFVELTMPYSVIRGEAFTLKATVLNYLPKCIRVSVHLEASPAFLAVPEKEQETYCICENGRQTVSWAVTPKSLGNVNFTVSAEAVESQELCGSEVPVVPEHGRKDTIIKPLLVEPEGLEKEVIFNSLLCPSGAKVSESLSLKLPANVVEESARASFSVLGDILGSAMRNTQNLLQMPYGCGEQNMALFAPNIYVLDYLNETQQLTAELKSKAILYLNTGYQRQLLYKHFDGSYSTFGEHHGNSEGNTWLTAFVLKSFAQARGYIFIDEAHITEALTWLAQKQKSNGCFRSSGSLLNNALKGGVDDEVTLSAYITIALLEMPLPVTHPVVRNALFCLDSAWKSAKEGSHGSHVYTKALLAYAFALAGNQERRTEVLRSLHEEAVKEDNTMHWTRPQKPRALTEDIYQPRAPSVEVEMTAYVLLAQVTAQPAPTPEDLDPATRIVKWISKQQNCQGGFSSTQDTVVALHALSRYGAATFTSATKVAQVTIQSSGTFSTKFQVENSNRLLLQQVSLPEVPGEYSMSVTGEGCVYLQTSLKYNILPKKDEFPFALEVHTLPQTCDGPKAHTSFQISLSVSYIGSRPASNMAIVDVKMVSGFIPLKPTVKMLERSNVSRTEVSNNHVLIYLDKVTDETLTLTFTVLQDIPVRDLKPAIVKVYDYYETDEFAVAEYSAPCSKDTGNA

Target ORF information:

RefSeq Version	XM_025283184.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis alpha-2-macroglobulin (LOC102402539), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283184.1

ORF Insert Sequence:

ATGGGGAAGA ACAAACTCCT TTATCCAAGT CTTACTCTTC TTCTGCTCTT GCTGCCGACA 
GATGCTTCAG TCTCTGGAAA ACCGCAATAC ATGGTGCTGG TCCCCTCCCT GCTCCATACT 
GGGACCCCTG AGAAGGGCTG CCTCCTTCTG AGCCACCTGA ATGAGACGGT GACCGTCAGC 
GCCTCCCTGG AGTCCGTCAG AGAGAACAGG AGCCTCTTCA CTGACGTGGT GGCAGAGAAG 
GACTTATTCC ACTGTGTCTC CTTCACTCTC CCAAGATCTC CAACCAGGCA AGAGGTAATG 
TTCCTTACCA TCCAAGTAAA AGGACCAACC CAAGAATTCA AGAAGCGGAC CACAGTGTTG 
GTTAAAAACG AAGAGAGCCT GGTCTTTGTC CAGACAGACA AACCCATCTA TAAACCAGAG 
CAGACAGTGA ACTTTCGTAT TGTCTTGTTG GATGAAAGCT TTCACCCCCT GAATGAGTTG 
GTTCCACTAG TATATGTTGA GGACCCCAAA GGAAATCGCA TCGCACAGTG GCAGAACCTC 
GAGGTAGAGA ATGGCCTCCA GCAGCTGACC TTCCCCCTCT CATCAGAGCC CTTTCAGGGC 
TCCTATAAGG TGGTGGTACA GAAGGGATCA GGTGGGACTG CAGAGCACCC CTTCACCGTG 
GAGGAATTCG TGCTTCCTAA GTTTGAAGTA CAAGTAAGAA TGCCAAAGAT AATCACCATC 
TTGGAAGAAG AGGTGAAAGT GTCTGTGTGC GGCCTATACA CTTATGGGAA GCCTGTCCCA 
GGACGTGTGA CTATGAACAT GTGCAGAAAG TACAGGAACC CTTCTAATTG TTATGGTGAA 
GAATCCAATG CTGTCTGTGA GAAGTTCAGT GGACAGCTGA ACAATGAGGG CTGCTTCTCT 
CAGCAAGTAA ACACCAAGAT CTTCCAGATG AAGAGACAAG AGTTTGAAAT GAAAATCGAA 
GTGGAAGCCA AGATCCAAGA AGAAGGAACA GAGGTGGAAT TAACTGGAAA GGGGGCCACT 
GAAATAACAA CAACCATCAC CAAACTCTCA TTTGTCACAG TGGACTCAAA CCTTAGACGA 
GGAATTCCTT TCACGGGGCA GGTGCTCCTA GTGGACGGGA AAGGTGTCCC CATGCCAAAT 
AAAGTCATCT TCATCACAGC AAACGAAGCC AACCACAACT CTAACACCAC CACTGATGAG 
CACGGCCTGG CACAGTTCTC CATCACCACC ACCAACATCA AGGGCACCTC CCTCAGTATC 
AGGGTCAAAT ACAAGGATCA CAGCCCCTGT TACGGCTACC AGTGGCTGTC AGAAGACCAT 
CAAGACGCTT ATCACAGTGC TAATCTTGTG TTCTCCCAAA GCAACAGCTT TGTCTACCTG 
GAGCCTTTGC CTCGTAAACT GCCCTGTGGC AAAACTCAGA CAGTCCAGGC ACATTATGTG 
CTGAAGGGAG AGGTCCTAAA GGATCTAAAG GAGCTCGTGT TCTATTACCT GATAATGGCA 
AAAGGAGGCA TTGTCCGATC TGGGACTCAC ACACTGCCCG TGGAGCAGGG AGAGATGCAA 
GGCCACTTTT CCATACCAGT CCCTGTGGAG TCAGACATTG CTCCTGTCGC TAGGCTGCTC 
ATCTATGCCA TTTTACCTGA TGGAGAAGTG GTAGGGGATT CTGCAAGATA TGAAGTTGAA 
CAATGTCTGA GCAACAAGGT GGGTTTGAAC TTCAGCCCCG GACAAAGTTT CCCAGCCTCC 
CAGGCCCACC TTCGAGTCAC CGCTTCCCCT CAGTCCCTCT GCGCCCTCCG CGCCGTGGAC 
CAAAGTGTGC TGCTCATGAG GCCCGAAGCC GAGCTCTCAG CAGCCACGGT TTATAACCTG 
CTACCAGTCA AGGACCTCAG CAGTTTCCCC TCAAGTGTAA ATCAACAGGA GGAAGACAGT 
GAAGGCTGCA TCAGTCGTGA CAATGTCTAT ATCAATGGAA TCATGTATTT CCCAGTATCA 
AATACAAATG AAAAAGATAT GTATAGCTTC CTGCAGGATA TGGGCTTGAA GGCATTTACC 
AACTCAAAGA TTCATAAACC CAAGATATGT CCACAGCCTG AAGAACATAG AGTACAGCAT 
CACACACTAT TAGCTTCTCC AGTCAGAGCT GAAATGGGAA GAGACAGTAA TTTTGTGCGT 
TTTGACGATA CTTCAGAGCC TCCCACAGAG ACTGTACGAA AGTACTTCCC TGAGACATGG 
ATCTGGGACT TGGTGGTTGT AAGCTCTTCA GGTGTGCATG AAGTAGAAGT AACAGTCCCT 
GATACCATCA CTGAGTGGAA GGCAGGGGCC CTCTGCCTGT CCAGAGACAC TGGACTTGGT 
CTCTCTCCGA CCGCCTCCCT CCGAGTCTTC CAGCCCTTCT TTGTGGAGCT TACAATGCCC 
TACTCTGTGA TCCGTGGAGA GGCCTTCACA CTCAAGGCCA CAGTGTTAAA CTACCTTCCC 
AAATGCATCC GGGTCAGTGT GCATCTAGAA GCCTCTCCTG CATTCCTAGC TGTTCCAGAA 
AAGGAACAAG AGACTTACTG CATCTGTGAA AATGGCCGGC AGACTGTGTC CTGGGCGGTA 
ACTCCAAAGT CCTTAGGGAA TGTGAATTTC ACAGTGAGTG CAGAGGCAGT GGAGTCTCAA 
GAGCTGTGTG GGAGTGAAGT GCCTGTGGTC CCTGAGCATG GAAGGAAAGA CACAATCATC 
AAGCCCCTAT TGGTTGAACC TGAAGGACTA GAGAAGGAAG TAATATTCAA CTCCCTGCTT 
TGTCCATCAG GTGCCAAGGT GTCTGAATCA TTATCTCTGA AGCTGCCAGC AAATGTGGTG 
GAAGAATCTG CCCGAGCCTC CTTCTCAGTT TTAGGAGACA TATTAGGCTC TGCCATGAGA 
AACACCCAAA ATCTCCTCCA AATGCCCTAT GGTTGTGGAG AACAGAATAT GGCCCTCTTT 
GCCCCTAACA TCTATGTTCT GGATTATCTA AATGAGACAC AGCAGCTGAC TGCAGAGCTC 
AAGTCCAAGG CTATCCTCTA CCTCAACACT GGTTACCAGA GACAGCTACT CTACAAGCAC 
TTCGATGGCT CTTACAGCAC CTTTGGGGAG CATCATGGCA ACAGTGAGGG CAATACTTGG 
CTCACGGCCT TTGTACTGAA GAGTTTTGCC CAGGCTCGAG GCTATATTTT CATTGATGAA 
GCACATATTA CTGAAGCTCT CACCTGGCTG GCCCAAAAGC AGAAGAGCAA TGGCTGTTTC 
AGGAGTTCTG GGTCTCTGCT TAACAATGCC CTCAAGGGTG GGGTGGATGA TGAAGTGACC 
CTCTCTGCCT ACATCACCAT CGCCCTTCTA GAGATGCCTC TCCCTGTCAC ACACCCTGTT 
GTCCGCAATG CCCTGTTCTG CCTGGACTCA GCTTGGAAGT CAGCCAAGGA AGGATCCCAC 
GGCAGCCACG TCTACACCAA GGCACTGTTG GCCTATGCCT TTGCCCTGGC GGGTAACCAG 
GAAAGGAGGA CAGAGGTACT GAGGTCACTG CATGAGGAAG CCGTGAAGGA AGATAATACC 
ATGCACTGGA CACGACCTCA GAAACCCAGG GCTCTCACGG AGGACATTTA CCAACCCAGG 
GCTCCGTCTG TGGAGGTGGA GATGACGGCC TACGTGCTCC TTGCTCAGGT CACGGCCCAG 
CCGGCCCCGA CCCCAGAGGA CCTCGACCCT GCCACGAGAA TCGTGAAGTG GATCTCAAAG 
CAGCAGAATT GCCAGGGGGG CTTCTCCTCC ACCCAGGACA CAGTGGTAGC TCTCCACGCC 
TTGTCCAGAT ACGGAGCAGC CACTTTCACC AGCGCTACGA AGGTTGCACA GGTGACCATC 
CAGTCTTCAG GGACATTTTC CACAAAATTC CAAGTGGAAA ACAGCAACCG CCTGTTACTA 
CAGCAGGTTT CACTGCCAGA AGTGCCTGGA GAGTACAGCA TGTCAGTCAC AGGAGAAGGA 
TGTGTTTACC TCCAGACATC TTTGAAATAC AATATTCTCC CGAAAAAAGA CGAGTTCCCA 
TTTGCTTTGG AGGTGCATAC TCTGCCCCAA ACTTGTGATG GACCCAAAGC CCACACCAGC 
TTCCAGATCT CACTGAGTGT CAGTTATATT GGAAGCCGTC CAGCCTCCAA TATGGCAATT 
GTTGATGTGA AGATGGTATC TGGCTTCATT CCCTTGAAAC CAACAGTGAA AATGCTAGAA 
AGATCTAATG TGAGCCGAAC AGAAGTTAGC AACAATCATG TTTTGATTTA TCTGGATAAG 
GTGACAGATG AGACCCTGAC CTTGACCTTC ACAGTTCTGC AAGACATCCC AGTAAGGGAT 
CTGAAACCGG CCATAGTGAA AGTCTATGAC TATTATGAGA CGGATGAGTT TGCAGTTGCT 
GAGTACAGTG CTCCGTGCAG CAAAGATACT GGAAATGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC102402539 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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