CNTRL gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006054143.1	XP_006054205.1	centriolin isoform X1
XM_025282346.1	XP_025138131.1	centriolin isoform X2
XM_025282349.1	XP_025138134.1	centriolin isoform X5
XM_025282353.1	XP_025138138.1	centriolin isoform X8
XM_006054146.1	XP_006054208.1	centriolin isoform X4
XM_006054147.1	XP_006054209.1	centriolin isoform X5
XM_025282345.1	XP_025138130.1	centriolin isoform X1
XM_025282351.1	XP_025138136.1	centriolin isoform X6
XM_025282352.1	XP_025138137.1	centriolin isoform X7
XM_025282354.1	XP_025138139.1	centriolin isoform X9
XM_006054145.1	XP_006054207.1	centriolin isoform X3
XM_006054148.1	XP_006054210.1	centriolin isoform X6
XM_025282344.1	XP_025138129.1	centriolin isoform X1
XM_025282347.1	XP_025138132.1	centriolin isoform X3
XM_006054144.1	XP_006054206.1	centriolin isoform X2
XM_006054149.1	XP_006054211.1	centriolin isoform X7
XM_025282348.1	XP_025138133.1	centriolin isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu11633	XM_006054148.1 Latest version!	Bubalus bubalis centriolin (CNTRL), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu11634	XM_006054149.1 Latest version!	Bubalus bubalis centriolin (CNTRL), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu63439	XM_025282352.1 Latest version!	Bubalus bubalis centriolin (CNTRL), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu63440	XM_025282353.1 Latest version!	Bubalus bubalis centriolin (CNTRL), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu11628	XM_006054143.1 Latest version!	Bubalus bubalis centriolin (CNTRL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBu11629	XM_006054144.1 Latest version!	Bubalus bubalis centriolin (CNTRL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OBu11630	XM_006054145.1 Latest version!	Bubalus bubalis centriolin (CNTRL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu11631	XM_006054146.1 Latest version!	Bubalus bubalis centriolin (CNTRL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu11632	XM_006054147.1 Latest version!	Bubalus bubalis centriolin (CNTRL), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu63433	XM_025282344.1 Latest version!	Bubalus bubalis centriolin (CNTRL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBu63433	XM_025282345.1 Latest version!	Bubalus bubalis centriolin (CNTRL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBu63434	XM_025282346.1 Latest version!	Bubalus bubalis centriolin (CNTRL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBu63435	XM_025282347.1 Latest version!	Bubalus bubalis centriolin (CNTRL), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBu63436	XM_025282348.1 Latest version!	Bubalus bubalis centriolin (CNTRL), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBu63437	XM_025282349.1 Latest version!	Bubalus bubalis centriolin (CNTRL), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBu63438	XM_025282351.1 Latest version!	Bubalus bubalis centriolin (CNTRL), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBu63441	XM_025282354.1 Latest version!	Bubalus bubalis centriolin (CNTRL), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

CNTRL ( XM_006054148.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006054210.1 Bubalus bubalis centriolin (CNTRL), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBu11633
Clone ID Related Accession (Same CDS sequence)	XM_006054148.1
Accession Version	XM_006054148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	centriolin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784589.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCTAAGACA GAAGACCATG 
GAACTAACGC GAGCATGTCA AAAACAGTAT GAACTGGAAC AGGAGTTGGC TTTTTACAAA 
ATCGATGCTA AATTTGAGCC ACTGAATTAT TATCCCTCAG AGTATGTTGA CGTTGATAAA 
GCCCCAGATG AAAGCCCTTA CATTGGCAAG TCCAGATACA AGAGAAATAT GTTCGCCACA 
GAGAGTTACA TTGTGAATAA TGCCCAGCCA GTTGAGATCA AGAGGATGGA GCCAGAGGAA 
GGGGAGCAAC TTAGAAACGA GCATGTGAGC TTGAAAGCCC ATATGCCACT GGACACGGAG 
CTGGAAGACA AAGAGAACAA AATAAGTACA GCACAAGCTC GACTATCAGC ACTGCATGAT 
GAAATAGAAA AGGCAGAACA ACAAATTTTA AGAGCCACTG AAGAATTTAA ACAACTGGAA 
GAAGCTGTAC AACTTAAAAA GATTTCAGAA GCAGAGAAAG ACTTTCTTCT CAAGCAGCTG 
AGTGGCAGGA TACAGCTTCT AAATAAATTA CGCCAGGAAG CTCTTGATCT AGAAATGCAG 
ATGGAAAAGC AAAGAGGAGA AATTGCTGAA AAGCAGGAGG AAATCAAGGA CCTCCAAAGA 
GTTATTGATA GTCTGGATTC CAAAGACCCA AAACATTGCC ATATGAAGGC TCAGAAAAGG 
GGTAAAGAAC AACAATTTGA CATTATGAAC AAGCAGTACA AACAACTTGA GAGCCGTTTG 
GACGAGATAC TTTCTAGAAT TGCTAAAGAA ACTGAAGAGA TTAAGGACCT TGAACAACAG 
CTTACTGAAG GCCAGATAGC AGCAAATGAA GCCCTGAAGA AGGACTTAGA AGGCGTCATC 
AGTGGCCTGC AAGAATACCT GGGCACTGTG AAGGGCCAGG CCGCTCAGGC CCAGAGCGAG 
TGCAGAAGGC TGCAGGATGA GAAAGAGGCG CTGCTGCAGA GGTTGGCCGA GGTCGAGCAG 
GAAAGGGACC AGCTGGAAGC AGTCGCCTTA GATGCAGAAG CTATGAGGAA GGAGCTTGCA 
GAGCTAGAAA GCGCCCTCCA AGAGCAGCAT GAGGTGAACG CGTCCCTGCA GCAGACCCAC 
GGAGACCTCA GTGCCTACGA GGCTGAGCTA GAGGCACAGC TGAGGATGAG GGATGCCGAA 
GCCAGCCAGC TCAAAGAAGA GCTGGGAGAA CTAACAAGGC TCAGCCAGCT AGAACAGTCA 
ACTCTACAAA CAGAACTTGA GAAAGAAAAA CAAGCCCTCA AGAACGCCCT GGGAAAAGCC 
CAGCTCTCGG AAGAAAAAGA ACAAGAAAAC AGTGAGCTCC GCACCCAGCT TAAACAGCTA 
CAGGATGACA ATAACCTGTT AAAACAGCAA CTTAAAGATT TCCAGAATCA CCTTAATCAG 
GTAGCTGATG GTCTGATTCG TCCAGAAGAA GTGGCAGCTC GTGTAGATGA AGTAAGGAGA 
AAATTGAAAT CAGGCGCTGG AGAGATGAGC AGAATCCACA GTCCTTCAGA TGTCTTAGGG 
AAAAGTCTTG CTGACTTGCA GATACAATTC AGTGAAATGC TGACACGTTC TCAGTGGGAG 
AAAGAGGAAG CCCAAGGGAG GGAAAGAAAA CTCCAAGAAG AAATGGCTCT GCATCAGGAG 
AAGCTGGTGG ATGGACAGGA AGCGTTCAGG CAGGCCTGTG AGAGAGCGCT AGAAGCAAGG 
ATTAATTTTG ACAAGAGGCA ACATGAAGCA AGAATTCAGC AGTTAGAGAA TGAGATTCAC 
TATTTACAAG AAAATCTAAA AAGTATGGAG GAAATCCAAG GGCTTACAGA TCTGCAGCTG 
CAGGAAGCTG ATGAAGAGAA GGAGAGAATT CTAGCCCAAC TCCAGGAGTT AGAGAAGAAG 
AAGAAACTTG AAGATGCCAA ATCTCAGGCA CAGGTTCTAG GTTTAGATAA AGAATTGAAG 
AAACTAAAGA AGGCTGTGGC CACCTCTGAT AAGATAGCCA CAGCTGAGCT CACCATCGCC 
AAAGACCAGC TCAAGTCCCT TCATGGAACT GTTATGAAAA TTAACCAGGA GCGTGCAGAG 
GACCTGCAGG AAGCAGAGCG GTTCAGCAGA AAGGCAGCAC AGGCCGCGAG GGATCTGACC 
CGAGCGGAAG CGGAGATCGA ACTCCTGCAG AACCTCCTCA GGGAAAGAGA GGAGCAGATG 
GAGAAGGTAG AGGCAGGCAC CCGAGGAGCA AGCTCACAGG CGCTGGAAAT CGAGAGGTGG 
AATGAGACCA TGGAGAGACA GAGGAGCGAG ATCGCAAGGC TACGGGACTC ACTAGACCGC 
ACCGGGGCGG ATAACAGAGG AGGCATTGAA AATATTTTGG AAGAAATAGC TGAACTCCGA 
CGTGAAGTTT CTTACCAGAA TGATTACATC AGCAGCATGG CAGATCCTTT CAAAAGACGA 
GGCTATTGGT ACTTTATGCC ACCACCATCA TCATCAAAAG TTTCCAGCCA CAGCTCCCAG 
GCCACAAAGG ACTCTGGTGT CGGGATGAAG TACGCAGCCT CGACTCCTAT TAGAAAACCA 
CAGCAGAACG GGAGGGAAGA CGGTGCCCCT CCAGCTGCCT CCGGATACTG GGTGTATTCC 
CCCATCAGGA GCGGCTTACA TAAATCGTTT TCAAATAAAG ATGCAGACAG TGGAGGAGAG 
AGCCGGGAGG AGAGCGAGCT GGATGACCAC GATGACCGCC CGTTCGTCCC TCCTCCTGGA 
TACATGATGT ACACCGTGTT CCCCGACGGC TCTCCTGTAC CACAGGGCAT GGCCCTCTAC 
GCACCGCCGC CTCCCTTGCC CAACAATAGC CGACCTCTCA GCCCTGGCAC TGTTGTGTAT 
GGCCCGCCCC CTGCTGGTGC CCCCATTGTC TACGGGCCCC CACCGCCCAG CTTCTCCGTC 
CCCCTCATCC CAGTGGGCGT GCTGCACTGC AACGTCCCGG AGCACCATGC CTTAGAGAAT 
GAGGTTTCTA GATTGGAAGA CATAATACAG CATTTAAAAT CAAAGAAGCA GGAACAGAGG 
ATGAGAGTAT GCAAGCGGCA GTCTGAGAAA GAAATAGAAG AACTGCATCA TAATATTGAT 
GAACTTTTGC AAGAGAAGAA AGACTTAGAG CACGAAGTAG AAGAATTACA TAGAACCATC 
CAGAAACATC AACAGCGAAA AGACTTCATT GATGGAAATG TTGAGAGTCT TATGAATGAA 
CTAAAAATAG AAAAATCACT CAAGCACCAT GAAGATATTG TAGATGAAAT TGAGTGCATC 
GAGAAGACTC TCCTGAAACG TCGAGCAGAG CTCAGGGAGG CTGACCGACT GCTGGCGGAA 
GCCGAGAGTG AACTTTCACG CACAACAGAG AAAACAAAAA ATGCTGTTGA AAAGTTCACT 
GATGCCAAGA GGAATTTGTT GCAAACTGAG AAAGATGCTG AGGAGTTAGA AAGGAGAGCT 
CAGGAAACAG CTGTTAGCCT TGTCAAGGCT GATCAACAGC TCAGATTGTT ACAGGCGGAC 
ACAAAGGATT TGGAGCAGCA CAAAACTGAG CAAGAAGAGA TCTTGAAAGA GATAAACAAA 
GTTGTTGCAG CAAAAGACTC AGACTTCCAG TGTTTAAACA CGAAGAAGGA GAAGCTAACA 
GAAGAGCTGC AGAAGCTGCA GAAAGACATC GAGACGGCAG CGCGCACGGA GGATCACCAC 
CTGCAGGTCC TGAGAGAGTC GGAGGCCCTC CTGCAGGCCA AGAGAGCGGA GCTGGACAAG 
CTGAGGAGCC AGGTAACGAG TCAGCAGCAG GAGATGGCTG TCTTGGACAG ACAGCTGGGG 
CATAAGAAGG AGGAGCTGCA TCTACTCCAG GGAAGCATGG TCCAGGCCAG AGCTGACCTC 
CAGGAAGCGC TGAGGCTGGG AGAAACTGAA GTAACTGAGA AGTGCAACCA CATCAGGGAA 
CTAAAAGGTC TTCTGGAAGA ACTGAGTTTT CAGAAAGGAG AACTGAATGT CCAGATTAGT 
GAGAAAAAAA ATCAACTTTC ACTTATAAAG CAAGAAATTG AAAAAGAGGA AGAAAATCTT 
CAGGTAGTTT TAGGGCAGAT GTCTAAACAT AAAACTGAAC TTAAGAATAT TCTGGACATG 
TTGCAACTTG AAAACAATGA GCTGCAAGCT TTGAAGCTAC AGCATGAGCA AAAGATGTCT 
GAGTTAGAGA AGGCTCAGGT TGAAGTGCTG GAGGAGAAAC TGGAGTTGGA GAATTTGCAG 
CAGACAGCCC TGCGACAGAA AGGGGAGATC GAGTGGCAGA AGCAGCTCTT AGAGAGGGAC 
AAGCGAGAGA TTGAGCGGAT AACTGCTGAG ACCCGAGCAT TGCAGTTGTG TGTTGAGTCT 
TTGTGCAAAC AAAAGGAAGA TCTTGAGGAG AAATGTGACA GCTGGGAGAA GAAGCTGGCA 
CAGACCAAAC GGGTTTTAGC AGCCACAGAA GAGAATAGCA AAGCAAAGCA ATCAGACTTA 
GAAAAGTTGG AATTGGATAT CAGAAAACTG GAACAGGAAC TAGACCAGCT AAACAGAAAC 
AAACTCTCAC TGCACAGAGA CATCGCGGCC ATGCAGCAGC AGGTCCAAGA AACAGAAGTG 
AGAAATTGTC CTAATATTAG GTATGGTGAT GGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006054210.1
CDS	129..5744
Translation	MKKGPQQKISKTKMPPSSHSPSPSSLTSNMRSRSLSPLNGSETLPFHSGRQWYGQVEIIGENSMLLDYHDQKEADSHAGVRYITEALVKKLTKQENLTLVKSLNLSLSKDGGKKFKYIENLEKCTKLEILNLSHNLIGKIEKVDKLLKLRELNLSYNKICKIEGIENMHNLQKLNLAGNEIEHIPAWLGKKLKCLRVLNLKANKISSLQDVSKLKPLQDLTSLILAENPVVTLPHYLQFTIFHLRSLESLEGQPVTTQDRQEAFERFSLEEVERLERDLEKKMMETEELKSKQAQFLEEIKNQDKLNRSLKEEAMLQKQSCEELESNLNTKNELLRQKTMELTRACQKQYELEQELAFYKIDAKFEPLNYYPSEYVDVDKAPDESPYIGKSRYKRNMFATESYIVNNAQPVEIKRMEPEEGEQLRNEHVSLKAHMPLDTELEDKENKISTAQARLSALHDEIEKAEQQILRATEEFKQLEEAVQLKKISEAEKDFLLKQLSGRIQLLNKLRQEALDLEMQMEKQRGEIAEKQEEIKDLQRVIDSLDSKDPKHCHMKAQKRGKEQQFDIMNKQYKQLESRLDEILSRIAKETEEIKDLEQQLTEGQIAANEALKKDLEGVISGLQEYLGTVKGQAAQAQSECRRLQDEKEALLQRLAEVEQERDQLEAVALDAEAMRKELAELESALQEQHEVNASLQQTHGDLSAYEAELEAQLRMRDAEASQLKEELGELTRLSQLEQSTLQTELEKEKQALKNALGKAQLSEEKEQENSELRTQLKQLQDDNNLLKQQLKDFQNHLNQVADGLIRPEEVAARVDEVRRKLKSGAGEMSRIHSPSDVLGKSLADLQIQFSEMLTRSQWEKEEAQGRERKLQEEMALHQEKLVDGQEAFRQACERALEARINFDKRQHEARIQQLENEIHYLQENLKSMEEIQGLTDLQLQEADEEKERILAQLQELEKKKKLEDAKSQAQVLGLDKELKKLKKAVATSDKIATAELTIAKDQLKSLHGTVMKINQERAEDLQEAERFSRKAAQAARDLTRAEAEIELLQNLLREREEQMEKVEAGTRGASSQALEIERWNETMERQRSEIARLRDSLDRTGADNRGGIENILEEIAELRREVSYQNDYISSMADPFKRRGYWYFMPPPSSSKVSSHSSQATKDSGVGMKYAASTPIRKPQQNGREDGAPPAASGYWVYSPIRSGLHKSFSNKDADSGGESREESELDDHDDRPFVPPPGYMMYTVFPDGSPVPQGMALYAPPPPLPNNSRPLSPGTVVYGPPPAGAPIVYGPPPPSFSVPLIPVGVLHCNVPEHHALENEVSRLEDIIQHLKSKKQEQRMRVCKRQSEKEIEELHHNIDELLQEKKDLEHEVEELHRTIQKHQQRKDFIDGNVESLMNELKIEKSLKHHEDIVDEIECIEKTLLKRRAELREADRLLAEAESELSRTTEKTKNAVEKFTDAKRNLLQTEKDAEELERRAQETAVSLVKADQQLRLLQADTKDLEQHKTEQEEILKEINKVVAAKDSDFQCLNTKKEKLTEELQKLQKDIETAARTEDHHLQVLRESEALLQAKRAELDKLRSQVTSQQQEMAVLDRQLGHKKEELHLLQGSMVQARADLQEALRLGETEVTEKCNHIRELKGLLEELSFQKGELNVQISEKKNQLSLIKQEIEKEEENLQVVLGQMSKHKTELKNILDMLQLENNELQALKLQHEQKMSELEKAQVEVLEEKLELENLQQTALRQKGEIEWQKQLLERDKREIERITAETRALQLCVESLCKQKEDLEEKCDSWEKKLAQTKRVLAATEENSKAKQSDLEKLELDIRKLEQELDQLNRNKLSLHRDIAAMQQQVQETEVRNCPNIRYGDG

Target ORF information:

RefSeq Version	XM_006054148.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centriolin (CNTRL), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006054148.1

ORF Insert Sequence:

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCTAAGACA GAAGACCATG 
GAACTAACGC GAGCATGTCA AAAACAGTAT GAACTGGAAC AGGAGTTGGC TTTTTACAAA 
ATCGATGCTA AATTTGAGCC ACTGAATTAT TATCCCTCAG AGTATGTTGA CGTTGATAAA 
GCCCCAGATG AAAGCCCTTA CATTGGCAAG TCCAGATACA AGAGAAATAT GTTCGCCACA 
GAGAGTTACA TTGTGAATAA TGCCCAGCCA GTTGAGATCA AGAGGATGGA GCCAGAGGAA 
GGGGAGCAAC TTAGAAACGA GCATGTGAGC TTGAAAGCCC ATATGCCACT GGACACGGAG 
CTGGAAGACA AAGAGAACAA AATAAGTACA GCACAAGCTC GACTATCAGC ACTGCATGAT 
GAAATAGAAA AGGCAGAACA ACAAATTTTA AGAGCCACTG AAGAATTTAA ACAACTGGAA 
GAAGCTGTAC AACTTAAAAA GATTTCAGAA GCAGAGAAAG ACTTTCTTCT CAAGCAGCTG 
AGTGGCAGGA TACAGCTTCT AAATAAATTA CGCCAGGAAG CTCTTGATCT AGAAATGCAG 
ATGGAAAAGC AAAGAGGAGA AATTGCTGAA AAGCAGGAGG AAATCAAGGA CCTCCAAAGA 
GTTATTGATA GTCTGGATTC CAAAGACCCA AAACATTGCC ATATGAAGGC TCAGAAAAGG 
GGTAAAGAAC AACAATTTGA CATTATGAAC AAGCAGTACA AACAACTTGA GAGCCGTTTG 
GACGAGATAC TTTCTAGAAT TGCTAAAGAA ACTGAAGAGA TTAAGGACCT TGAACAACAG 
CTTACTGAAG GCCAGATAGC AGCAAATGAA GCCCTGAAGA AGGACTTAGA AGGCGTCATC 
AGTGGCCTGC AAGAATACCT GGGCACTGTG AAGGGCCAGG CCGCTCAGGC CCAGAGCGAG 
TGCAGAAGGC TGCAGGATGA GAAAGAGGCG CTGCTGCAGA GGTTGGCCGA GGTCGAGCAG 
GAAAGGGACC AGCTGGAAGC AGTCGCCTTA GATGCAGAAG CTATGAGGAA GGAGCTTGCA 
GAGCTAGAAA GCGCCCTCCA AGAGCAGCAT GAGGTGAACG CGTCCCTGCA GCAGACCCAC 
GGAGACCTCA GTGCCTACGA GGCTGAGCTA GAGGCACAGC TGAGGATGAG GGATGCCGAA 
GCCAGCCAGC TCAAAGAAGA GCTGGGAGAA CTAACAAGGC TCAGCCAGCT AGAACAGTCA 
ACTCTACAAA CAGAACTTGA GAAAGAAAAA CAAGCCCTCA AGAACGCCCT GGGAAAAGCC 
CAGCTCTCGG AAGAAAAAGA ACAAGAAAAC AGTGAGCTCC GCACCCAGCT TAAACAGCTA 
CAGGATGACA ATAACCTGTT AAAACAGCAA CTTAAAGATT TCCAGAATCA CCTTAATCAG 
GTAGCTGATG GTCTGATTCG TCCAGAAGAA GTGGCAGCTC GTGTAGATGA AGTAAGGAGA 
AAATTGAAAT CAGGCGCTGG AGAGATGAGC AGAATCCACA GTCCTTCAGA TGTCTTAGGG 
AAAAGTCTTG CTGACTTGCA GATACAATTC AGTGAAATGC TGACACGTTC TCAGTGGGAG 
AAAGAGGAAG CCCAAGGGAG GGAAAGAAAA CTCCAAGAAG AAATGGCTCT GCATCAGGAG 
AAGCTGGTGG ATGGACAGGA AGCGTTCAGG CAGGCCTGTG AGAGAGCGCT AGAAGCAAGG 
ATTAATTTTG ACAAGAGGCA ACATGAAGCA AGAATTCAGC AGTTAGAGAA TGAGATTCAC 
TATTTACAAG AAAATCTAAA AAGTATGGAG GAAATCCAAG GGCTTACAGA TCTGCAGCTG 
CAGGAAGCTG ATGAAGAGAA GGAGAGAATT CTAGCCCAAC TCCAGGAGTT AGAGAAGAAG 
AAGAAACTTG AAGATGCCAA ATCTCAGGCA CAGGTTCTAG GTTTAGATAA AGAATTGAAG 
AAACTAAAGA AGGCTGTGGC CACCTCTGAT AAGATAGCCA CAGCTGAGCT CACCATCGCC 
AAAGACCAGC TCAAGTCCCT TCATGGAACT GTTATGAAAA TTAACCAGGA GCGTGCAGAG 
GACCTGCAGG AAGCAGAGCG GTTCAGCAGA AAGGCAGCAC AGGCCGCGAG GGATCTGACC 
CGAGCGGAAG CGGAGATCGA ACTCCTGCAG AACCTCCTCA GGGAAAGAGA GGAGCAGATG 
GAGAAGGTAG AGGCAGGCAC CCGAGGAGCA AGCTCACAGG CGCTGGAAAT CGAGAGGTGG 
AATGAGACCA TGGAGAGACA GAGGAGCGAG ATCGCAAGGC TACGGGACTC ACTAGACCGC 
ACCGGGGCGG ATAACAGAGG AGGCATTGAA AATATTTTGG AAGAAATAGC TGAACTCCGA 
CGTGAAGTTT CTTACCAGAA TGATTACATC AGCAGCATGG CAGATCCTTT CAAAAGACGA 
GGCTATTGGT ACTTTATGCC ACCACCATCA TCATCAAAAG TTTCCAGCCA CAGCTCCCAG 
GCCACAAAGG ACTCTGGTGT CGGGATGAAG TACGCAGCCT CGACTCCTAT TAGAAAACCA 
CAGCAGAACG GGAGGGAAGA CGGTGCCCCT CCAGCTGCCT CCGGATACTG GGTGTATTCC 
CCCATCAGGA GCGGCTTACA TAAATCGTTT TCAAATAAAG ATGCAGACAG TGGAGGAGAG 
AGCCGGGAGG AGAGCGAGCT GGATGACCAC GATGACCGCC CGTTCGTCCC TCCTCCTGGA 
TACATGATGT ACACCGTGTT CCCCGACGGC TCTCCTGTAC CACAGGGCAT GGCCCTCTAC 
GCACCGCCGC CTCCCTTGCC CAACAATAGC CGACCTCTCA GCCCTGGCAC TGTTGTGTAT 
GGCCCGCCCC CTGCTGGTGC CCCCATTGTC TACGGGCCCC CACCGCCCAG CTTCTCCGTC 
CCCCTCATCC CAGTGGGCGT GCTGCACTGC AACGTCCCGG AGCACCATGC CTTAGAGAAT 
GAGGTTTCTA GATTGGAAGA CATAATACAG CATTTAAAAT CAAAGAAGCA GGAACAGAGG 
ATGAGAGTAT GCAAGCGGCA GTCTGAGAAA GAAATAGAAG AACTGCATCA TAATATTGAT 
GAACTTTTGC AAGAGAAGAA AGACTTAGAG CACGAAGTAG AAGAATTACA TAGAACCATC 
CAGAAACATC AACAGCGAAA AGACTTCATT GATGGAAATG TTGAGAGTCT TATGAATGAA 
CTAAAAATAG AAAAATCACT CAAGCACCAT GAAGATATTG TAGATGAAAT TGAGTGCATC 
GAGAAGACTC TCCTGAAACG TCGAGCAGAG CTCAGGGAGG CTGACCGACT GCTGGCGGAA 
GCCGAGAGTG AACTTTCACG CACAACAGAG AAAACAAAAA ATGCTGTTGA AAAGTTCACT 
GATGCCAAGA GGAATTTGTT GCAAACTGAG AAAGATGCTG AGGAGTTAGA AAGGAGAGCT 
CAGGAAACAG CTGTTAGCCT TGTCAAGGCT GATCAACAGC TCAGATTGTT ACAGGCGGAC 
ACAAAGGATT TGGAGCAGCA CAAAACTGAG CAAGAAGAGA TCTTGAAAGA GATAAACAAA 
GTTGTTGCAG CAAAAGACTC AGACTTCCAG TGTTTAAACA CGAAGAAGGA GAAGCTAACA 
GAAGAGCTGC AGAAGCTGCA GAAAGACATC GAGACGGCAG CGCGCACGGA GGATCACCAC 
CTGCAGGTCC TGAGAGAGTC GGAGGCCCTC CTGCAGGCCA AGAGAGCGGA GCTGGACAAG 
CTGAGGAGCC AGGTAACGAG TCAGCAGCAG GAGATGGCTG TCTTGGACAG ACAGCTGGGG 
CATAAGAAGG AGGAGCTGCA TCTACTCCAG GGAAGCATGG TCCAGGCCAG AGCTGACCTC 
CAGGAAGCGC TGAGGCTGGG AGAAACTGAA GTAACTGAGA AGTGCAACCA CATCAGGGAA 
CTAAAAGGTC TTCTGGAAGA ACTGAGTTTT CAGAAAGGAG AACTGAATGT CCAGATTAGT 
GAGAAAAAAA ATCAACTTTC ACTTATAAAG CAAGAAATTG AAAAAGAGGA AGAAAATCTT 
CAGGTAGTTT TAGGGCAGAT GTCTAAACAT AAAACTGAAC TTAAGAATAT TCTGGACATG 
TTGCAACTTG AAAACAATGA GCTGCAAGCT TTGAAGCTAC AGCATGAGCA AAAGATGTCT 
GAGTTAGAGA AGGCTCAGGT TGAAGTGCTG GAGGAGAAAC TGGAGTTGGA GAATTTGCAG 
CAGACAGCCC TGCGACAGAA AGGGGAGATC GAGTGGCAGA AGCAGCTCTT AGAGAGGGAC 
AAGCGAGAGA TTGAGCGGAT AACTGCTGAG ACCCGAGCAT TGCAGTTGTG TGTTGAGTCT 
TTGTGCAAAC AAAAGGAAGA TCTTGAGGAG AAATGTGACA GCTGGGAGAA GAAGCTGGCA 
CAGACCAAAC GGGTTTTAGC AGCCACAGAA GAGAATAGCA AAGCAAAGCA ATCAGACTTA 
GAAAAGTTGG AATTGGATAT CAGAAAACTG GAACAGGAAC TAGACCAGCT AAACAGAAAC 
AAACTCTCAC TGCACAGAGA CATCGCGGCC ATGCAGCAGC AGGTCCAAGA AACAGAAGTG 
AGAAATTGTC CTAATATTAG GTATGGTGAT GGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTRL ( XM_006054149.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006054211.1 Bubalus bubalis centriolin (CNTRL), transcript variant X7, mRNA.

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CloneID	OBu11634
Clone ID Related Accession (Same CDS sequence)	XM_006054149.1
Accession Version	XM_006054149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	centriolin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784589.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCTC AGAAAAGGGG TAAAGAACAA CAATTTGACA TTATGAACAA GCAGTACAAA 
CAACTTGAGA GCCGTTTGGA CGAGATACTT TCTAGAATTG CTAAAGAAAC TGAAGAGATT 
AAGGACCTTG AACAACAGCT TACTGAAGGC CAGATAGCAG CAAATGAAGC CCTGAAGAAG 
GACTTAGAAG GCGTCATCAG TGGCCTGCAA GAATACCTGG GCACTGTGAA GGGCCAGGCC 
GCTCAGGCCC AGAGCGAGTG CAGAAGGCTG CAGGATGAGA AAGAGGCGCT GCTGCAGAGG 
TTGGCCGAGG TCGAGCAGGA AAGGGACCAG CTGGAAGCAG TCGCCTTAGA TGCAGAAGCT 
ATGAGGAAGG AGCTTGCAGA GCTAGAAAGC GCCCTCCAAG AGCAGCATGA GGTGAACGCG 
TCCCTGCAGC AGACCCACGG AGACCTCAGT GCCTACGAGG CTGAGCTAGA GGCACAGCTG 
AGGATGAGGG ATGCCGAAGC CAGCCAGCTC AAAGAAGAGC TGGGAGAACT AACAAGGCTC 
AGCCAGCTAG AACAGTCAAC TCTACAAACA GAACTTGAGA AAGAAAAACA AGCCCTCAAG 
AACGCCCTGG GAAAAGCCCA GCTCTCGGAA GAAAAAGAAC AAGAAAACAG TGAGCTCCGC 
ACCCAGCTTA AACAGCTACA GGATGACAAT AACCTGTTAA AACAGCAACT TAAAGATTTC 
CAGAATCACC TTAATCAGGT AGCTGATGGT CTGATTCGTC CAGAAGAAGT GGCAGCTCGT 
GTAGATGAAG TAAGGAGAAA ATTGAAATCA GGCGCTGGAG AGATGAGCAG AATCCACAGT 
CCTTCAGATG TCTTAGGGAA AAGTCTTGCT GACTTGCAGA TACAATTCAG TGAAATGCTG 
ACACGTTCTC AGTGGGAGAA AGAGGAAGCC CAAGGGAGGG AAAGAAAACT CCAAGAAGAA 
ATGGCTCTGC ATCAGGAGAA GCTGGTGGAT GGACAGGAAG CGTTCAGGCA GGCCTGTGAG 
AGAGCGCTAG AAGCAAGGAT TAATTTTGAC AAGAGGCAAC ATGAAGCAAG AATTCAGCAG 
TTAGAGAATG AGATTCACTA TTTACAAGAA AATCTAAAAA GTATGGAGGA AATCCAAGGG 
CTTACAGATC TGCAGCTGCA GGAAGCTGAT GAAGAGAAGG AGAGAATTCT AGCCCAACTC 
CAGGAGTTAG AGAAGAAGAA GAAACTTGAA GATGCCAAAT CTCAGGCACA GGTTCTAGGT 
TTAGATAAAG AATTGAAGAA ACTAAAGAAG GCTGTGGCCA CCTCTGATAA GATAGCCACA 
GCTGAGCTCA CCATCGCCAA AGACCAGCTC AAGTCCCTTC ATGGAACTGT TATGAAAATT 
AACCAGGAGC GTGCAGAGGA CCTGCAGGAA GCAGAGCGGT TCAGCAGAAA GGCAGCACAG 
GCCGCGAGGG ATCTGACCCG AGCGGAAGCG GAGATCGAAC TCCTGCAGAA CCTCCTCAGG 
GAAAGAGAGG AGCAGATGGA GAAGGTAGAG GCAGGCACCC GAGGAGCAAG CTCACAGGCG 
CTGGAAATCG AGAGGTGGAA TGAGACCATG GAGAGACAGA GGAGCGAGAT CGCAAGGCTA 
CGGGACTCAC TAGACCGCAC CGGGGCGGAT AACAGAGGAG GCATTGAAAA TATTTTGGAA 
GAAATAGCTG AACTCCGACG TGAAGTTTCT TACCAGAATG ATTACATCAG CAGCATGGCA 
GATCCTTTCA AAAGACGAGG CTATTGGTAC TTTATGCCAC CACCATCATC ATCAAAAGTT 
TCCAGCCACA GCTCCCAGGC CACAAAGGAC TCTGGTGTCG GGATGAAGTA CGCAGCCTCG 
ACTCCTATTA GAAAACCACA GCAGAACGGG AGGGAAGACG GTGCCCCTCC AGCTGCCTCC 
GGATACTGGG TGTATTCCCC CATCAGGAGC GGCTTACATA AATCGTTTTC AAATAAAGAT 
GCAGACAGTG GAGGAGAGAG CCGGGAGGAG AGCGAGCTGG ATGACCACGA TGACCGCCCG 
TTCGTCCCTC CTCCTGGATA CATGATGTAC ACCGTGTTCC CCGACGGCTC TCCTGTACCA 
CAGGGCATGG CCCTCTACGC ACCGCCGCCT CCCTTGCCCA ACAATAGCCG ACCTCTCAGC 
CCTGGCACTG TTGTGTATGG CCCGCCCCCT GCTGGTGCCC CCATTGTCTA CGGGCCCCCA 
CCGCCCAGCT TCTCCGTCCC CCTCATCCCA GTGGGCGTGC TGCACTGCAA CGTCCCGGAG 
CACCATGCCT TAGAGAATGA GGTTTCTAGA TTGGAAGACA TAATACAGCA TTTAAAATCA 
AAGAAGCAGG AACAGAGGAT GAGAGTATGC AAGCGGCAGT CTGAGAAAGA AATAGAAGAA 
CTGCATCATA ATATTGATGA ACTTTTGCAA GAGAAGAAAG ACTTAGAGCA CGAAGTAGAA 
GAATTACATA GAACCATCCA GAAACATCAA CAGCGAAAAG ACTTCATTGA TGGAAATGTT 
GAGAGTCTTA TGAATGAACT AAAAATAGAA AAATCACTCA AGCACCATGA AGATATTGTA 
GATGAAATTG AGTGCATCGA GAAGACTCTC CTGAAACGTC GAGCAGAGCT CAGGGAGGCT 
GACCGACTGC TGGCGGAAGC CGAGAGTGAA CTTTCACGCA CAACAGAGAA AACAAAAAAT 
GCTGTTGAAA AGTTCACTGA TGCCAAGAGG AATTTGTTGC AAACTGAGAA AGATGCTGAG 
GAGTTAGAAA GGAGAGCTCA GGAAACAGCT GTTAGCCTTG TCAAGGCTGA TCAACAGCTC 
AGATTGTTAC AGGCGGACAC AAAGGATTTG GAGCAGCACA AAACTGAGCA AGAAGAGATC 
TTGAAAGAGA TAAACAAAGT TGTTGCAGCA AAAGACTCAG ACTTCCAGTG TTTAAACACG 
AAGAAGGAGA AGCTAACAGA AGAGCTGCAG AAGCTGCAGA AAGACATCGA GACGGCAGCG 
CGCACGGAGG ATCACCACCT GCAGGTCCTG AGAGAGTCGG AGGCCCTCCT GCAGGCCAAG 
AGAGCGGAGC TGGACAAGCT GAGGAGCCAG GTAACGAGTC AGCAGCAGGA GATGGCTGTC 
TTGGACAGAC AGCTGGGGCA TAAGAAGGAG GAGCTGCATC TACTCCAGGG AAGCATGGTC 
CAGGCCAGAG CTGACCTCCA GGAAGCGCTG AGGCTGGGAG AAACTGAAGT AACTGAGAAG 
TGCAACCACA TCAGGGAACT AAAAGGTCTT CTGGAAGAAC TGAGTTTTCA GAAAGGAGAA 
CTGAATGTCC AGATTAGTGA GAAAAAAAAT CAACTTTCAC TTATAAAGCA AGAAATTGAA 
AAAGAGGAAG AAAATCTTCA GGTAGTTTTA GGGCAGATGT CTAAACATAA AACTGAACTT 
AAGAATATTC TGGACATGTT GCAACTTGAA AACAATGAGC TGCAAGCTTT GAAGCTACAG 
CATGAGCAAA AGATGTCTGA GTTAGAGAAG GCTCAGGTTG AAGTGCTGGA GGAGAAACTG 
GAGTTGGAGA ATTTGCAGCA GACAGCCCTG CGACAGAAAG GGGAGATCGA GTGGCAGAAG 
CAGCTCTTAG AGAGGGACAA GCGAGAGATT GAGCGGATAA CTGCTGAGAC CCGAGCATTG 
CAGTTGTGTG TTGAGTCTTT GTGCAAACAA AAGGAAGATC TTGAGGAGAA ATGTGACAGC 
TGGGAGAAGA AGCTGGCACA GACCAAACGG GTTTTAGCAG CCACAGAAGA GAATAGCAAA 
GCAAAGCAAT CAGACTTAGA AAAGTTGGAA TTGGATATCA GAAAACTGGA ACAGGAACTA 
GACCAGCTAA ACAGAAACAA ACTCTCACTG CACAGAGACA TCGCGGCCAT GCAGCAGCAG 
GTCCAAGAAA AACGAGAAGC AGTAAACTCA TTACAGGAGG AACTAGCTGA TGTCCAAGCC 
CATTTGAACC TAGCGAAACA GGACCTGCTC CACACCACCA AGCGCCAGGA TGTCCTGCTT 
GGCGAGCAGA CCAGGCTGCA GGAGGACCTC CAGGAACGCA GAAAGAAGTT GGAAGTCTGC 
CAAAAAGAAG AGGCAACAAA GCGGCAGCAC CTGCAAGCGC TTCAGACGGA GATGGAGGCG 
AACGAGGCCG AGCTGGCCCG GCAGGAGACG ACATTTCAGA GACTCCAGAA AGAGAGAGAA 
AGTGAAGAAA AAAAGTTAGA AGCCAGTCGA GCGACTCTGC AGGAGCAGCA GCAGCAGCTG 
GAAAAGGAAA TCGCAGACCA GAAGAACGTG CTGGACCAGG TGCTCAGCCA GGTGCTGGGG 
GCCAAGGAGC GCGCCAGGAC TCTGCAGCAG GAGGAGAGGT GGGGTGAGAC CCTGGAGAAG 
ACGCTCTCCC AGACCCAGCA ACAGCTCTCA GAACGGGAGC AGCAGTTGTT GGAAAAGTCA 
GCTGAGCTGC TGGCTCTTCA GAAAGAGGCA GACTCGATGA GGGCAGACTT CAGCCTCTTG 
CGAAACCAGT TCTTGACGGA GAGGAAGAAA GCCGAGAAGC AGGTAGCCAG CCTCAGGGAA 
GCGCTCAAGA CCCAGCGCAG CCAGCTGGAG AAGACCCTTC TCGAGCAGAA GCAGGAGAAC 
AGCTCCATGC AGAAGGAGAT GGCGACTATC GAGCAGGTGG CGCAGGACAA CCACGAGCGG 
GCCCGGCGCC TGATGCGGGA GCTCAGCCAG ATGCAGCAGG AGTACGCGGA GCTCAAGAAG 
CAGATGGTAA ACCAGAAAGA TCTGGAAAGA AGACAAGTAG AAATCAGTGA AGCGATGAGG 
ACACTTAAGT CTGAAGTGAA AGACGAGATC AGAGCCAGCC TGAAGAACCT CAACCAGTTT 
CTCCCGGAGC TGCCAGCAGA CCTGGAGGCC ATTCTGGAAA GGAGCGAACA CCTAGACGGA 
GGGCTGGAGA GCTTGAAGGA GAACTTTCCA TTCGCCCTGA GTGAGAAGCC ATCACCTTTG 
GAAGAGAAAC TACATTTTTC TCGCGTTCAC ATAATGGATG AGCACTGGCG TGGAGAAGCG 
CTCCGGGAGA AGCTGCGGCA CCGTGAGGAC CGACTCAAGG CCCAGCTCCG ACACTGTATG 
TCCAAGCAGG CAGAGGTGTT AAGCAAAGGG AAGCGGCAGA CCGAGGGCAC GTTACACAGC 
CTGAGGAGGC AGGTCGACGC CTTAGGGGAG CTGGTCACCA GCACGTCTGT GGAGTCCACG 
TCGTCAGCCA CTCTGTCTCA GCTCGAATCT TCCCTTGCAG AGGACTCCCA TCTTGGACCA 
AGTCAGAGCT CCTTCCATCA AGTTCCACAA GGTCCATCAC AATCAGTAGA CAGTTACCGA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006054211.1
CDS	181..5526
Translation	MKAQKRGKEQQFDIMNKQYKQLESRLDEILSRIAKETEEIKDLEQQLTEGQIAANEALKKDLEGVISGLQEYLGTVKGQAAQAQSECRRLQDEKEALLQRLAEVEQERDQLEAVALDAEAMRKELAELESALQEQHEVNASLQQTHGDLSAYEAELEAQLRMRDAEASQLKEELGELTRLSQLEQSTLQTELEKEKQALKNALGKAQLSEEKEQENSELRTQLKQLQDDNNLLKQQLKDFQNHLNQVADGLIRPEEVAARVDEVRRKLKSGAGEMSRIHSPSDVLGKSLADLQIQFSEMLTRSQWEKEEAQGRERKLQEEMALHQEKLVDGQEAFRQACERALEARINFDKRQHEARIQQLENEIHYLQENLKSMEEIQGLTDLQLQEADEEKERILAQLQELEKKKKLEDAKSQAQVLGLDKELKKLKKAVATSDKIATAELTIAKDQLKSLHGTVMKINQERAEDLQEAERFSRKAAQAARDLTRAEAEIELLQNLLREREEQMEKVEAGTRGASSQALEIERWNETMERQRSEIARLRDSLDRTGADNRGGIENILEEIAELRREVSYQNDYISSMADPFKRRGYWYFMPPPSSSKVSSHSSQATKDSGVGMKYAASTPIRKPQQNGREDGAPPAASGYWVYSPIRSGLHKSFSNKDADSGGESREESELDDHDDRPFVPPPGYMMYTVFPDGSPVPQGMALYAPPPPLPNNSRPLSPGTVVYGPPPAGAPIVYGPPPPSFSVPLIPVGVLHCNVPEHHALENEVSRLEDIIQHLKSKKQEQRMRVCKRQSEKEIEELHHNIDELLQEKKDLEHEVEELHRTIQKHQQRKDFIDGNVESLMNELKIEKSLKHHEDIVDEIECIEKTLLKRRAELREADRLLAEAESELSRTTEKTKNAVEKFTDAKRNLLQTEKDAEELERRAQETAVSLVKADQQLRLLQADTKDLEQHKTEQEEILKEINKVVAAKDSDFQCLNTKKEKLTEELQKLQKDIETAARTEDHHLQVLRESEALLQAKRAELDKLRSQVTSQQQEMAVLDRQLGHKKEELHLLQGSMVQARADLQEALRLGETEVTEKCNHIRELKGLLEELSFQKGELNVQISEKKNQLSLIKQEIEKEEENLQVVLGQMSKHKTELKNILDMLQLENNELQALKLQHEQKMSELEKAQVEVLEEKLELENLQQTALRQKGEIEWQKQLLERDKREIERITAETRALQLCVESLCKQKEDLEEKCDSWEKKLAQTKRVLAATEENSKAKQSDLEKLELDIRKLEQELDQLNRNKLSLHRDIAAMQQQVQEKREAVNSLQEELADVQAHLNLAKQDLLHTTKRQDVLLGEQTRLQEDLQERRKKLEVCQKEEATKRQHLQALQTEMEANEAELARQETTFQRLQKERESEEKKLEASRATLQEQQQQLEKEIADQKNVLDQVLSQVLGAKERARTLQQEERWGETLEKTLSQTQQQLSEREQQLLEKSAELLALQKEADSMRADFSLLRNQFLTERKKAEKQVASLREALKTQRSQLEKTLLEQKQENSSMQKEMATIEQVAQDNHERARRLMRELSQMQQEYAELKKQMVNQKDLERRQVEISEAMRTLKSEVKDEIRASLKNLNQFLPELPADLEAILERSEHLDGGLESLKENFPFALSEKPSPLEEKLHFSRVHIMDEHWRGEALREKLRHREDRLKAQLRHCMSKQAEVLSKGKRQTEGTLHSLRRQVDALGELVTSTSVESTSSATLSQLESSLAEDSHLGPSQSSFHQVPQGPSQSVDSYRH

Target ORF information:

RefSeq Version	XM_006054149.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centriolin (CNTRL), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006054149.1

ORF Insert Sequence:

ATGAAGGCTC AGAAAAGGGG TAAAGAACAA CAATTTGACA TTATGAACAA GCAGTACAAA 
CAACTTGAGA GCCGTTTGGA CGAGATACTT TCTAGAATTG CTAAAGAAAC TGAAGAGATT 
AAGGACCTTG AACAACAGCT TACTGAAGGC CAGATAGCAG CAAATGAAGC CCTGAAGAAG 
GACTTAGAAG GCGTCATCAG TGGCCTGCAA GAATACCTGG GCACTGTGAA GGGCCAGGCC 
GCTCAGGCCC AGAGCGAGTG CAGAAGGCTG CAGGATGAGA AAGAGGCGCT GCTGCAGAGG 
TTGGCCGAGG TCGAGCAGGA AAGGGACCAG CTGGAAGCAG TCGCCTTAGA TGCAGAAGCT 
ATGAGGAAGG AGCTTGCAGA GCTAGAAAGC GCCCTCCAAG AGCAGCATGA GGTGAACGCG 
TCCCTGCAGC AGACCCACGG AGACCTCAGT GCCTACGAGG CTGAGCTAGA GGCACAGCTG 
AGGATGAGGG ATGCCGAAGC CAGCCAGCTC AAAGAAGAGC TGGGAGAACT AACAAGGCTC 
AGCCAGCTAG AACAGTCAAC TCTACAAACA GAACTTGAGA AAGAAAAACA AGCCCTCAAG 
AACGCCCTGG GAAAAGCCCA GCTCTCGGAA GAAAAAGAAC AAGAAAACAG TGAGCTCCGC 
ACCCAGCTTA AACAGCTACA GGATGACAAT AACCTGTTAA AACAGCAACT TAAAGATTTC 
CAGAATCACC TTAATCAGGT AGCTGATGGT CTGATTCGTC CAGAAGAAGT GGCAGCTCGT 
GTAGATGAAG TAAGGAGAAA ATTGAAATCA GGCGCTGGAG AGATGAGCAG AATCCACAGT 
CCTTCAGATG TCTTAGGGAA AAGTCTTGCT GACTTGCAGA TACAATTCAG TGAAATGCTG 
ACACGTTCTC AGTGGGAGAA AGAGGAAGCC CAAGGGAGGG AAAGAAAACT CCAAGAAGAA 
ATGGCTCTGC ATCAGGAGAA GCTGGTGGAT GGACAGGAAG CGTTCAGGCA GGCCTGTGAG 
AGAGCGCTAG AAGCAAGGAT TAATTTTGAC AAGAGGCAAC ATGAAGCAAG AATTCAGCAG 
TTAGAGAATG AGATTCACTA TTTACAAGAA AATCTAAAAA GTATGGAGGA AATCCAAGGG 
CTTACAGATC TGCAGCTGCA GGAAGCTGAT GAAGAGAAGG AGAGAATTCT AGCCCAACTC 
CAGGAGTTAG AGAAGAAGAA GAAACTTGAA GATGCCAAAT CTCAGGCACA GGTTCTAGGT 
TTAGATAAAG AATTGAAGAA ACTAAAGAAG GCTGTGGCCA CCTCTGATAA GATAGCCACA 
GCTGAGCTCA CCATCGCCAA AGACCAGCTC AAGTCCCTTC ATGGAACTGT TATGAAAATT 
AACCAGGAGC GTGCAGAGGA CCTGCAGGAA GCAGAGCGGT TCAGCAGAAA GGCAGCACAG 
GCCGCGAGGG ATCTGACCCG AGCGGAAGCG GAGATCGAAC TCCTGCAGAA CCTCCTCAGG 
GAAAGAGAGG AGCAGATGGA GAAGGTAGAG GCAGGCACCC GAGGAGCAAG CTCACAGGCG 
CTGGAAATCG AGAGGTGGAA TGAGACCATG GAGAGACAGA GGAGCGAGAT CGCAAGGCTA 
CGGGACTCAC TAGACCGCAC CGGGGCGGAT AACAGAGGAG GCATTGAAAA TATTTTGGAA 
GAAATAGCTG AACTCCGACG TGAAGTTTCT TACCAGAATG ATTACATCAG CAGCATGGCA 
GATCCTTTCA AAAGACGAGG CTATTGGTAC TTTATGCCAC CACCATCATC ATCAAAAGTT 
TCCAGCCACA GCTCCCAGGC CACAAAGGAC TCTGGTGTCG GGATGAAGTA CGCAGCCTCG 
ACTCCTATTA GAAAACCACA GCAGAACGGG AGGGAAGACG GTGCCCCTCC AGCTGCCTCC 
GGATACTGGG TGTATTCCCC CATCAGGAGC GGCTTACATA AATCGTTTTC AAATAAAGAT 
GCAGACAGTG GAGGAGAGAG CCGGGAGGAG AGCGAGCTGG ATGACCACGA TGACCGCCCG 
TTCGTCCCTC CTCCTGGATA CATGATGTAC ACCGTGTTCC CCGACGGCTC TCCTGTACCA 
CAGGGCATGG CCCTCTACGC ACCGCCGCCT CCCTTGCCCA ACAATAGCCG ACCTCTCAGC 
CCTGGCACTG TTGTGTATGG CCCGCCCCCT GCTGGTGCCC CCATTGTCTA CGGGCCCCCA 
CCGCCCAGCT TCTCCGTCCC CCTCATCCCA GTGGGCGTGC TGCACTGCAA CGTCCCGGAG 
CACCATGCCT TAGAGAATGA GGTTTCTAGA TTGGAAGACA TAATACAGCA TTTAAAATCA 
AAGAAGCAGG AACAGAGGAT GAGAGTATGC AAGCGGCAGT CTGAGAAAGA AATAGAAGAA 
CTGCATCATA ATATTGATGA ACTTTTGCAA GAGAAGAAAG ACTTAGAGCA CGAAGTAGAA 
GAATTACATA GAACCATCCA GAAACATCAA CAGCGAAAAG ACTTCATTGA TGGAAATGTT 
GAGAGTCTTA TGAATGAACT AAAAATAGAA AAATCACTCA AGCACCATGA AGATATTGTA 
GATGAAATTG AGTGCATCGA GAAGACTCTC CTGAAACGTC GAGCAGAGCT CAGGGAGGCT 
GACCGACTGC TGGCGGAAGC CGAGAGTGAA CTTTCACGCA CAACAGAGAA AACAAAAAAT 
GCTGTTGAAA AGTTCACTGA TGCCAAGAGG AATTTGTTGC AAACTGAGAA AGATGCTGAG 
GAGTTAGAAA GGAGAGCTCA GGAAACAGCT GTTAGCCTTG TCAAGGCTGA TCAACAGCTC 
AGATTGTTAC AGGCGGACAC AAAGGATTTG GAGCAGCACA AAACTGAGCA AGAAGAGATC 
TTGAAAGAGA TAAACAAAGT TGTTGCAGCA AAAGACTCAG ACTTCCAGTG TTTAAACACG 
AAGAAGGAGA AGCTAACAGA AGAGCTGCAG AAGCTGCAGA AAGACATCGA GACGGCAGCG 
CGCACGGAGG ATCACCACCT GCAGGTCCTG AGAGAGTCGG AGGCCCTCCT GCAGGCCAAG 
AGAGCGGAGC TGGACAAGCT GAGGAGCCAG GTAACGAGTC AGCAGCAGGA GATGGCTGTC 
TTGGACAGAC AGCTGGGGCA TAAGAAGGAG GAGCTGCATC TACTCCAGGG AAGCATGGTC 
CAGGCCAGAG CTGACCTCCA GGAAGCGCTG AGGCTGGGAG AAACTGAAGT AACTGAGAAG 
TGCAACCACA TCAGGGAACT AAAAGGTCTT CTGGAAGAAC TGAGTTTTCA GAAAGGAGAA 
CTGAATGTCC AGATTAGTGA GAAAAAAAAT CAACTTTCAC TTATAAAGCA AGAAATTGAA 
AAAGAGGAAG AAAATCTTCA GGTAGTTTTA GGGCAGATGT CTAAACATAA AACTGAACTT 
AAGAATATTC TGGACATGTT GCAACTTGAA AACAATGAGC TGCAAGCTTT GAAGCTACAG 
CATGAGCAAA AGATGTCTGA GTTAGAGAAG GCTCAGGTTG AAGTGCTGGA GGAGAAACTG 
GAGTTGGAGA ATTTGCAGCA GACAGCCCTG CGACAGAAAG GGGAGATCGA GTGGCAGAAG 
CAGCTCTTAG AGAGGGACAA GCGAGAGATT GAGCGGATAA CTGCTGAGAC CCGAGCATTG 
CAGTTGTGTG TTGAGTCTTT GTGCAAACAA AAGGAAGATC TTGAGGAGAA ATGTGACAGC 
TGGGAGAAGA AGCTGGCACA GACCAAACGG GTTTTAGCAG CCACAGAAGA GAATAGCAAA 
GCAAAGCAAT CAGACTTAGA AAAGTTGGAA TTGGATATCA GAAAACTGGA ACAGGAACTA 
GACCAGCTAA ACAGAAACAA ACTCTCACTG CACAGAGACA TCGCGGCCAT GCAGCAGCAG 
GTCCAAGAAA AACGAGAAGC AGTAAACTCA TTACAGGAGG AACTAGCTGA TGTCCAAGCC 
CATTTGAACC TAGCGAAACA GGACCTGCTC CACACCACCA AGCGCCAGGA TGTCCTGCTT 
GGCGAGCAGA CCAGGCTGCA GGAGGACCTC CAGGAACGCA GAAAGAAGTT GGAAGTCTGC 
CAAAAAGAAG AGGCAACAAA GCGGCAGCAC CTGCAAGCGC TTCAGACGGA GATGGAGGCG 
AACGAGGCCG AGCTGGCCCG GCAGGAGACG ACATTTCAGA GACTCCAGAA AGAGAGAGAA 
AGTGAAGAAA AAAAGTTAGA AGCCAGTCGA GCGACTCTGC AGGAGCAGCA GCAGCAGCTG 
GAAAAGGAAA TCGCAGACCA GAAGAACGTG CTGGACCAGG TGCTCAGCCA GGTGCTGGGG 
GCCAAGGAGC GCGCCAGGAC TCTGCAGCAG GAGGAGAGGT GGGGTGAGAC CCTGGAGAAG 
ACGCTCTCCC AGACCCAGCA ACAGCTCTCA GAACGGGAGC AGCAGTTGTT GGAAAAGTCA 
GCTGAGCTGC TGGCTCTTCA GAAAGAGGCA GACTCGATGA GGGCAGACTT CAGCCTCTTG 
CGAAACCAGT TCTTGACGGA GAGGAAGAAA GCCGAGAAGC AGGTAGCCAG CCTCAGGGAA 
GCGCTCAAGA CCCAGCGCAG CCAGCTGGAG AAGACCCTTC TCGAGCAGAA GCAGGAGAAC 
AGCTCCATGC AGAAGGAGAT GGCGACTATC GAGCAGGTGG CGCAGGACAA CCACGAGCGG 
GCCCGGCGCC TGATGCGGGA GCTCAGCCAG ATGCAGCAGG AGTACGCGGA GCTCAAGAAG 
CAGATGGTAA ACCAGAAAGA TCTGGAAAGA AGACAAGTAG AAATCAGTGA AGCGATGAGG 
ACACTTAAGT CTGAAGTGAA AGACGAGATC AGAGCCAGCC TGAAGAACCT CAACCAGTTT 
CTCCCGGAGC TGCCAGCAGA CCTGGAGGCC ATTCTGGAAA GGAGCGAACA CCTAGACGGA 
GGGCTGGAGA GCTTGAAGGA GAACTTTCCA TTCGCCCTGA GTGAGAAGCC ATCACCTTTG 
GAAGAGAAAC TACATTTTTC TCGCGTTCAC ATAATGGATG AGCACTGGCG TGGAGAAGCG 
CTCCGGGAGA AGCTGCGGCA CCGTGAGGAC CGACTCAAGG CCCAGCTCCG ACACTGTATG 
TCCAAGCAGG CAGAGGTGTT AAGCAAAGGG AAGCGGCAGA CCGAGGGCAC GTTACACAGC 
CTGAGGAGGC AGGTCGACGC CTTAGGGGAG CTGGTCACCA GCACGTCTGT GGAGTCCACG 
TCGTCAGCCA CTCTGTCTCA GCTCGAATCT TCCCTTGCAG AGGACTCCCA TCTTGGACCA 
AGTCAGAGCT CCTTCCATCA AGTTCCACAA GGTCCATCAC AATCAGTAGA CAGTTACCGA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTRL ( XM_025282352.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025138137.1 Bubalus bubalis centriolin (CNTRL), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBu63439
Clone ID Related Accession (Same CDS sequence)	XM_025282352.1
Accession Version	XM_025282352.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	centriolin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCCCTTGTC TCGGTTTCTT 
GCGGACCCTT GTCTGCCGAT TCTTTATTCT TCCTTAAAGC TAAGACAGAA GACCATGGAA 
CTAACGCGAG CATGTCAAAA ACAGTATGAA CTGGAACAGG AGTTGGCTTT TTACAAAATC 
GATGCTAAAT TTGAGCCACT GAATTATTAT CCCTCAGAGT ATGTTGACGT TGATAAAGCC 
CCAGATGAAA GCCCTTACAT TGGCAAGTCC AGATACAAGA GAAATATGTT CGCCACAGAG 
AGTTACATTG TGAATAATGC CCAGCCAGTT GAGATCAAGA GGATGGAGCC AGAGGAAGGG 
GAGCAACTTA GAAACGAGCA TGTGAGCTTG AAAGCCCATA TGCCACTGGA CACGGAGCTG 
GAAGACAAAG AGAACAAAAT AAGTACAGCA CAAGCTCGAC TATCAGCACT GCATGATGAA 
ATAGAAAAGG CAGAACAACA AATTTTAAGA GCCACTGAAG AATTTAAACA ACTGGAAGAA 
GCTGTACAAC TTAAAAAGAT TTCAGAAGCA GAGAAAGACT TTCTTCTCAA GCAGCTGAGT 
GGCAGGATAC AGCTTCTAAA TAAATTACGC CAGGAAGCTC TTGATCTAGA AATGCAGATG 
GAAAAGCAAA GAGGAGAAAT TGCTGAAAAG CAGGAGGAAA TCAAGGACCT CCAAAGAGTT 
ATTGATAGTC TGGATTCCAA AGACCCAAAA CATTGCCATA TGAAGGCTCA GAAAAGGGGT 
AAAGAACAAC AATTTGACAT TATGAACAAG CAGTACAAAC AACTTGAGAG CCGTTTGGAC 
GAGATACTTT CTAGAATTGC TAAAGAAACT GAAGAGATTA AGGACCTTGA ACAACAGCTT 
ACTGAAGGCC AGATAGCAGC AAATGAAGCC CTGAAGAAGG ACTTAGAAGG CGTCATCAGT 
GGCCTGCAAG AATACCTGGG CACTGTGAAG GGCCAGGCCG CTCAGGCCCA GAGCGAGTGC 
AGAAGGCTGC AGGATGAGAA AGAGGCGCTG CTGCAGAGGT TGGCCGAGGT CGAGCAGGAA 
AGGGACCAGC TGGAAGCAGT CGCCTTAGAT GCAGAAGCTA TGAGGAAGGA GCTTGCAGAG 
CTAGAAAGCG CCCTCCAAGA GCAGCATGAG GTGAACGCGT CCCTGCAGCA GACCCACGGA 
GACCTCAGTG CCTACGAGGC TGAGCTAGAG GCACAGCTGA GGATGAGGGA TGCCGAAGCC 
AGCCAGCTCA AAGAAGAGCT GGGAGAACTA ACAAGGCTCA GCCAGCTAGA ACAGTCAACT 
CTACAAACAG AACTTGAGAA AGAAAAACAA GCCCTCAAGA ACGCCCTGGG AAAAGCCCAG 
CTCTCGGAAG AAAAAGAACA AGAAAACAGT GAGCTCCGCA CCCAGCTTAA ACAGCTACAG 
GATGACAATA ACCTGTTAAA ACAGCAACTT AAAGATTTCC AGAATCACCT TAATCAGGTA 
GCTGATGGTC TGATTCGTCC AGAAGAAGTG GCAGCTCGTG TAGATGAAGT AAGGAGAAAA 
TTGAAATCAG GCGCTGGAGA GATGAGCAGA ATCCACAGTC CTTCAGATGT CTTAGGGAAA 
AGTCTTGCTG ACTTGCAGAT ACAATTCAGT GAAATGCTGA CACGTTCTCA GTGGGAGAAA 
GAGGAAGCCC AAGGGAGGGA AAGAAAACTC CAAGAAGAAA TGGCTCTGCA TCAGGAGAAG 
CTGGTGGATG GACAGGAAGC GTTCAGGCAG GCCTGTGAGA GAGCGCTAGA AGCAAGGATT 
AATTTTGACA AGAGGCAACA TGAAGCAAGA ATTCAGCAGT TAGAGAATGA GATTCACTAT 
TTACAAGAAA ATCTAAAAAG TATGGAGGAA ATCCAAGGGC TTACAGATCT GCAGCTGCAG 
GAAGCTGATG AAGAGAAGGA GAGAATTCTA GCCCAACTCC AGGAGTTAGA GAAGAAGAAG 
AAACTTGAAG ATGCCAAATC TCAGGCACAG GTTCTAGGTT TAGATAAAGA ATTGAAGAAA 
CTAAAGAAGG CTGTGGCCAC CTCTGATAAG ATAGCCACAG CTGAGCTCAC CATCGCCAAA 
GACCAGCTCA AGTCCCTTCA TGGAACTGTT ATGAAAATTA ACCAGGAGCG TGCAGAGGAC 
CTGCAGGAAG CAGAGCGGTT CAGCAGAAAG GCAGCACAGG CCGCGAGGGA TCTGACCCGA 
GCGGAAGCGG AGATCGAACT CCTGCAGAAC CTCCTCAGGG AGAGAGAGGA GCAGATGGAG 
AAGGTAGAGG CAGGCACCCG AGGAGCAAGC TCACAGGCGC TGGAAATCGA GAGGTGGAAT 
GAGACCATGG AGAGACAGAG GAGCGAGATC GCAAGGCTAC GGGACTCACT AGACCGCACC 
GGGGCGGATA ACAGAGGAGG CATTGAAAAT ATTTTGGAAG AAATAGCTGA ACTCCGACGT 
GAAGTTTCTT ACCAGAATGA TTACATCAGC AGCATGGCAG ATCCTTTCAA AAGACGAGGC 
TATTGGTACT TTATGCCACC ACCATCATCA TCAAAAGTTT CCAGCCACAG CTCCCAGGCC 
ACAAAGGACT CTGGTGTCGG GATGAAGTAC GCAGCCTCGA CTCCTATTAG AAAACCACAG 
CAGAACGGGA GGGAAGACGG TGCCCCTCCA GCTGCCTCCG GATACTGGGT GTATTCCCCC 
ATCAGGAGCG GCTTACATAA ATCGTTTTCA AATAAAGATG CAGACAGTGG AGGAGAGAGC 
CGGGAGGAGA GCGAGCTGGA TGACCACGAT GACCGCCCGT TCGTCCCTCC TCCTGGATAC 
ATGATGTACA CCGTGTTCCC CGACGGCTCT CCTGTACCAC AGGGCATGGC CCTCTACGCA 
CCGCCGCCTC CCTTGCCCAA CAATAGCCGA CCTCTCAGCC CTGGCACTGT TGTGTATGGC 
CCGCCCCCTG CTGGTGCCCC CATTGTCTAC GGGCCCCCAC CGCCCAGCTT CTCCGTCCCC 
CTCATCCCAG TGGGCGTGCT GCACTGCAAC GTCCCGGAGC ACCATGCCTT AGAGAATGAG 
GTTTCTAGAT TGGAAGACAT AATACAGCAT TTAAAATCAA AGAAGCAGGA ACAGAGGATG 
AGAGTATGCA AGCGGCAGTC TGAGAAAGAA ATAGAAGAAC TGCATCATAA TATTGATGAA 
CTTTTGCAAG AGAAGAAAGA CTTAGAGCAC GAAGTAGAAG AATTACATAG AACCATCCAG 
AAACATCAAC AGCAAAAAGA CTTCATTGAT GGAAATGTTG AGAGTCTTAT GAATGAACTA 
AAAATAGAAA AATCACTCAA GCACCATGAA GATATTGTAG ATGAAATTGA GTGCATCGAG 
AAGACTCTCC TGAAACGTCG AGCAGAGCTC AGGGAGGCTG ACCGACTGCT GGCGGAAGCC 
GAGAGTGAAC TTTCACGCAC AACAGAGAAA ACAAAAAATG CTGTTGAAAA GTTCACTGAT 
GCCAAGAGGA ATTTGTTGCA AACTGAGAAA GATGCTGAGG AGTTAGAAAG GAGAGCTCAG 
GAAACAGCTG TTAGCCTTGT CAAGGCTGAT CAACAGCTCA GATTGTTACA GGCGGACACA 
AAGGATTTGG AGCAGCACAA AACTGAGCAA GAAGAGATCT TGAAAGAGAT AAACAAAGTT 
GTTGCAGCAA AAGACTCAGA CTTCCAGTGT TTAAACACGA AGAAGGAGAA GCTAACAGAA 
GAGCTGCAGA AGCTGCAGAA AGACATCGAG ACGGCAGCGC GCACGGAGGA TCACCACCTG 
CAGGTCCTGA GAGAGTCGGA GGCCCTCCTG CAGGCCAAGA GAGCGGAGCT GGACAAGCTG 
AGGAGCCAGG TAACGAGTCA GCAGCAGGAG ATGGCTGTCT TGGACAGACA GCTGGGGCAT 
AAGAAGGAGG AGCTGCATCT ACTCCAGGGA AGCATGGTCC AGGCCAGAGC TGACCTCCAG 
GAAGCGCTGA GGCTGGGAGA AACTGAAGTA ACTGAGAAGT GCAACCACAT CAGGGAACTA 
AAAGGTCTTC TGGAAGAACT GAGTTTTCAG AAAGGAGAAC TGAATGTCCA GATTAGTGAG 
AAAAAAAATC AACTTTCACT TATAAAGCAA GAAATTGAAA AAGAGGAAGA AAATCTTCAG 
GTAGTTTTAG GGCAGATGTC TAAACATAAA ACTGAACTTA AGAATATTCT GGACATGTTG 
CAACTTGAAA ACAATGAGCT GCAAGCTTTG AAGCTACAGC ATGAGCAAAA GATGTCTGAG 
TTAGAGAAGG CTCAGGTTGA AGTGCTGGAG GAGAAACTGG AGTTGGAGAA TTTGCAGCAG 
ACAGCCCTGC GACAGAAAGG GGAGATCGAG TGGCAGAAGC AGCTCTTAGA GAGGGACAAG 
CGAGAGATTG AGCGGATAAC TGCTGAGACC CGAGCATTGC AGTTGTGTGT TGAGTCTTTG 
TGCAAACAAA AGGAAGATCT TGAGGAGAAA TGTGACAGCT GGGAGAAGAA GCTGGCACAG 
ACCAAACGGG TTTTAGCAGC CACAGAAGAG AATAGCAAAG CAAAGCAATC AGACTTAGAA 
AAGTTGGAAT TGGATATCAG AAAACTGGAA CAGGAACTAG ACCAGCTAAA CAGAAACAAA 
CTCTCACTGC ACAGAGACAT CGCGGCCATG CAGCAGCAGG TCCAAGAAAC AGAAGTGAGA 
AATTGTCCTA ATATTAGAAA AACGAGAAGC AGTAAACTCA TTACAGGAGG AACTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025138137.1
CDS	365..6064
Translation	MKKGPQQKISKTKMPPSSHSPSPSSLTSNMRSRSLSPLNGSETLPFHSGRQWYGQVEIIGENSMLLDYHDQKEADSHAGVRYITEALVKKLTKQENLTLVKSLNLSLSKDGGKKFKYIENLEKCTKLEILNLSHNLIGKIEKVDKLLKLRELNLSYNKICKIEGIENMHNLQKLNLAGNEIEHIPAWLGKKLKCLRVLNLKANKISSLQDVSKLKPLQDLTSLILAENPVVTLPHYLQFTIFHLRSLESLEGQPVTTQDRQEAFERFSLEEVERLERDLEKKMMETEELKSKQAQFLEEIKNQDKLNRSLKEEAMLQKQSCEELESNLNTKNELPLSRFLADPCLPILYSSLKLRQKTMELTRACQKQYELEQELAFYKIDAKFEPLNYYPSEYVDVDKAPDESPYIGKSRYKRNMFATESYIVNNAQPVEIKRMEPEEGEQLRNEHVSLKAHMPLDTELEDKENKISTAQARLSALHDEIEKAEQQILRATEEFKQLEEAVQLKKISEAEKDFLLKQLSGRIQLLNKLRQEALDLEMQMEKQRGEIAEKQEEIKDLQRVIDSLDSKDPKHCHMKAQKRGKEQQFDIMNKQYKQLESRLDEILSRIAKETEEIKDLEQQLTEGQIAANEALKKDLEGVISGLQEYLGTVKGQAAQAQSECRRLQDEKEALLQRLAEVEQERDQLEAVALDAEAMRKELAELESALQEQHEVNASLQQTHGDLSAYEAELEAQLRMRDAEASQLKEELGELTRLSQLEQSTLQTELEKEKQALKNALGKAQLSEEKEQENSELRTQLKQLQDDNNLLKQQLKDFQNHLNQVADGLIRPEEVAARVDEVRRKLKSGAGEMSRIHSPSDVLGKSLADLQIQFSEMLTRSQWEKEEAQGRERKLQEEMALHQEKLVDGQEAFRQACERALEARINFDKRQHEARIQQLENEIHYLQENLKSMEEIQGLTDLQLQEADEEKERILAQLQELEKKKKLEDAKSQAQVLGLDKELKKLKKAVATSDKIATAELTIAKDQLKSLHGTVMKINQERAEDLQEAERFSRKAAQAARDLTRAEAEIELLQNLLREREEQMEKVEAGTRGASSQALEIERWNETMERQRSEIARLRDSLDRTGADNRGGIENILEEIAELRREVSYQNDYISSMADPFKRRGYWYFMPPPSSSKVSSHSSQATKDSGVGMKYAASTPIRKPQQNGREDGAPPAASGYWVYSPIRSGLHKSFSNKDADSGGESREESELDDHDDRPFVPPPGYMMYTVFPDGSPVPQGMALYAPPPPLPNNSRPLSPGTVVYGPPPAGAPIVYGPPPPSFSVPLIPVGVLHCNVPEHHALENEVSRLEDIIQHLKSKKQEQRMRVCKRQSEKEIEELHHNIDELLQEKKDLEHEVEELHRTIQKHQQQKDFIDGNVESLMNELKIEKSLKHHEDIVDEIECIEKTLLKRRAELREADRLLAEAESELSRTTEKTKNAVEKFTDAKRNLLQTEKDAEELERRAQETAVSLVKADQQLRLLQADTKDLEQHKTEQEEILKEINKVVAAKDSDFQCLNTKKEKLTEELQKLQKDIETAARTEDHHLQVLRESEALLQAKRAELDKLRSQVTSQQQEMAVLDRQLGHKKEELHLLQGSMVQARADLQEALRLGETEVTEKCNHIRELKGLLEELSFQKGELNVQISEKKNQLSLIKQEIEKEEENLQVVLGQMSKHKTELKNILDMLQLENNELQALKLQHEQKMSELEKAQVEVLEEKLELENLQQTALRQKGEIEWQKQLLERDKREIERITAETRALQLCVESLCKQKEDLEEKCDSWEKKLAQTKRVLAATEENSKAKQSDLEKLELDIRKLEQELDQLNRNKLSLHRDIAAMQQQVQETEVRNCPNIRKTRSSKLITGGTS

Target ORF information:

RefSeq Version	XM_025282352.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centriolin (CNTRL), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025282352.1

ORF Insert Sequence:

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCCCTTGTC TCGGTTTCTT 
GCGGACCCTT GTCTGCCGAT TCTTTATTCT TCCTTAAAGC TAAGACAGAA GACCATGGAA 
CTAACGCGAG CATGTCAAAA ACAGTATGAA CTGGAACAGG AGTTGGCTTT TTACAAAATC 
GATGCTAAAT TTGAGCCACT GAATTATTAT CCCTCAGAGT ATGTTGACGT TGATAAAGCC 
CCAGATGAAA GCCCTTACAT TGGCAAGTCC AGATACAAGA GAAATATGTT CGCCACAGAG 
AGTTACATTG TGAATAATGC CCAGCCAGTT GAGATCAAGA GGATGGAGCC AGAGGAAGGG 
GAGCAACTTA GAAACGAGCA TGTGAGCTTG AAAGCCCATA TGCCACTGGA CACGGAGCTG 
GAAGACAAAG AGAACAAAAT AAGTACAGCA CAAGCTCGAC TATCAGCACT GCATGATGAA 
ATAGAAAAGG CAGAACAACA AATTTTAAGA GCCACTGAAG AATTTAAACA ACTGGAAGAA 
GCTGTACAAC TTAAAAAGAT TTCAGAAGCA GAGAAAGACT TTCTTCTCAA GCAGCTGAGT 
GGCAGGATAC AGCTTCTAAA TAAATTACGC CAGGAAGCTC TTGATCTAGA AATGCAGATG 
GAAAAGCAAA GAGGAGAAAT TGCTGAAAAG CAGGAGGAAA TCAAGGACCT CCAAAGAGTT 
ATTGATAGTC TGGATTCCAA AGACCCAAAA CATTGCCATA TGAAGGCTCA GAAAAGGGGT 
AAAGAACAAC AATTTGACAT TATGAACAAG CAGTACAAAC AACTTGAGAG CCGTTTGGAC 
GAGATACTTT CTAGAATTGC TAAAGAAACT GAAGAGATTA AGGACCTTGA ACAACAGCTT 
ACTGAAGGCC AGATAGCAGC AAATGAAGCC CTGAAGAAGG ACTTAGAAGG CGTCATCAGT 
GGCCTGCAAG AATACCTGGG CACTGTGAAG GGCCAGGCCG CTCAGGCCCA GAGCGAGTGC 
AGAAGGCTGC AGGATGAGAA AGAGGCGCTG CTGCAGAGGT TGGCCGAGGT CGAGCAGGAA 
AGGGACCAGC TGGAAGCAGT CGCCTTAGAT GCAGAAGCTA TGAGGAAGGA GCTTGCAGAG 
CTAGAAAGCG CCCTCCAAGA GCAGCATGAG GTGAACGCGT CCCTGCAGCA GACCCACGGA 
GACCTCAGTG CCTACGAGGC TGAGCTAGAG GCACAGCTGA GGATGAGGGA TGCCGAAGCC 
AGCCAGCTCA AAGAAGAGCT GGGAGAACTA ACAAGGCTCA GCCAGCTAGA ACAGTCAACT 
CTACAAACAG AACTTGAGAA AGAAAAACAA GCCCTCAAGA ACGCCCTGGG AAAAGCCCAG 
CTCTCGGAAG AAAAAGAACA AGAAAACAGT GAGCTCCGCA CCCAGCTTAA ACAGCTACAG 
GATGACAATA ACCTGTTAAA ACAGCAACTT AAAGATTTCC AGAATCACCT TAATCAGGTA 
GCTGATGGTC TGATTCGTCC AGAAGAAGTG GCAGCTCGTG TAGATGAAGT AAGGAGAAAA 
TTGAAATCAG GCGCTGGAGA GATGAGCAGA ATCCACAGTC CTTCAGATGT CTTAGGGAAA 
AGTCTTGCTG ACTTGCAGAT ACAATTCAGT GAAATGCTGA CACGTTCTCA GTGGGAGAAA 
GAGGAAGCCC AAGGGAGGGA AAGAAAACTC CAAGAAGAAA TGGCTCTGCA TCAGGAGAAG 
CTGGTGGATG GACAGGAAGC GTTCAGGCAG GCCTGTGAGA GAGCGCTAGA AGCAAGGATT 
AATTTTGACA AGAGGCAACA TGAAGCAAGA ATTCAGCAGT TAGAGAATGA GATTCACTAT 
TTACAAGAAA ATCTAAAAAG TATGGAGGAA ATCCAAGGGC TTACAGATCT GCAGCTGCAG 
GAAGCTGATG AAGAGAAGGA GAGAATTCTA GCCCAACTCC AGGAGTTAGA GAAGAAGAAG 
AAACTTGAAG ATGCCAAATC TCAGGCACAG GTTCTAGGTT TAGATAAAGA ATTGAAGAAA 
CTAAAGAAGG CTGTGGCCAC CTCTGATAAG ATAGCCACAG CTGAGCTCAC CATCGCCAAA 
GACCAGCTCA AGTCCCTTCA TGGAACTGTT ATGAAAATTA ACCAGGAGCG TGCAGAGGAC 
CTGCAGGAAG CAGAGCGGTT CAGCAGAAAG GCAGCACAGG CCGCGAGGGA TCTGACCCGA 
GCGGAAGCGG AGATCGAACT CCTGCAGAAC CTCCTCAGGG AGAGAGAGGA GCAGATGGAG 
AAGGTAGAGG CAGGCACCCG AGGAGCAAGC TCACAGGCGC TGGAAATCGA GAGGTGGAAT 
GAGACCATGG AGAGACAGAG GAGCGAGATC GCAAGGCTAC GGGACTCACT AGACCGCACC 
GGGGCGGATA ACAGAGGAGG CATTGAAAAT ATTTTGGAAG AAATAGCTGA ACTCCGACGT 
GAAGTTTCTT ACCAGAATGA TTACATCAGC AGCATGGCAG ATCCTTTCAA AAGACGAGGC 
TATTGGTACT TTATGCCACC ACCATCATCA TCAAAAGTTT CCAGCCACAG CTCCCAGGCC 
ACAAAGGACT CTGGTGTCGG GATGAAGTAC GCAGCCTCGA CTCCTATTAG AAAACCACAG 
CAGAACGGGA GGGAAGACGG TGCCCCTCCA GCTGCCTCCG GATACTGGGT GTATTCCCCC 
ATCAGGAGCG GCTTACATAA ATCGTTTTCA AATAAAGATG CAGACAGTGG AGGAGAGAGC 
CGGGAGGAGA GCGAGCTGGA TGACCACGAT GACCGCCCGT TCGTCCCTCC TCCTGGATAC 
ATGATGTACA CCGTGTTCCC CGACGGCTCT CCTGTACCAC AGGGCATGGC CCTCTACGCA 
CCGCCGCCTC CCTTGCCCAA CAATAGCCGA CCTCTCAGCC CTGGCACTGT TGTGTATGGC 
CCGCCCCCTG CTGGTGCCCC CATTGTCTAC GGGCCCCCAC CGCCCAGCTT CTCCGTCCCC 
CTCATCCCAG TGGGCGTGCT GCACTGCAAC GTCCCGGAGC ACCATGCCTT AGAGAATGAG 
GTTTCTAGAT TGGAAGACAT AATACAGCAT TTAAAATCAA AGAAGCAGGA ACAGAGGATG 
AGAGTATGCA AGCGGCAGTC TGAGAAAGAA ATAGAAGAAC TGCATCATAA TATTGATGAA 
CTTTTGCAAG AGAAGAAAGA CTTAGAGCAC GAAGTAGAAG AATTACATAG AACCATCCAG 
AAACATCAAC AGCAAAAAGA CTTCATTGAT GGAAATGTTG AGAGTCTTAT GAATGAACTA 
AAAATAGAAA AATCACTCAA GCACCATGAA GATATTGTAG ATGAAATTGA GTGCATCGAG 
AAGACTCTCC TGAAACGTCG AGCAGAGCTC AGGGAGGCTG ACCGACTGCT GGCGGAAGCC 
GAGAGTGAAC TTTCACGCAC AACAGAGAAA ACAAAAAATG CTGTTGAAAA GTTCACTGAT 
GCCAAGAGGA ATTTGTTGCA AACTGAGAAA GATGCTGAGG AGTTAGAAAG GAGAGCTCAG 
GAAACAGCTG TTAGCCTTGT CAAGGCTGAT CAACAGCTCA GATTGTTACA GGCGGACACA 
AAGGATTTGG AGCAGCACAA AACTGAGCAA GAAGAGATCT TGAAAGAGAT AAACAAAGTT 
GTTGCAGCAA AAGACTCAGA CTTCCAGTGT TTAAACACGA AGAAGGAGAA GCTAACAGAA 
GAGCTGCAGA AGCTGCAGAA AGACATCGAG ACGGCAGCGC GCACGGAGGA TCACCACCTG 
CAGGTCCTGA GAGAGTCGGA GGCCCTCCTG CAGGCCAAGA GAGCGGAGCT GGACAAGCTG 
AGGAGCCAGG TAACGAGTCA GCAGCAGGAG ATGGCTGTCT TGGACAGACA GCTGGGGCAT 
AAGAAGGAGG AGCTGCATCT ACTCCAGGGA AGCATGGTCC AGGCCAGAGC TGACCTCCAG 
GAAGCGCTGA GGCTGGGAGA AACTGAAGTA ACTGAGAAGT GCAACCACAT CAGGGAACTA 
AAAGGTCTTC TGGAAGAACT GAGTTTTCAG AAAGGAGAAC TGAATGTCCA GATTAGTGAG 
AAAAAAAATC AACTTTCACT TATAAAGCAA GAAATTGAAA AAGAGGAAGA AAATCTTCAG 
GTAGTTTTAG GGCAGATGTC TAAACATAAA ACTGAACTTA AGAATATTCT GGACATGTTG 
CAACTTGAAA ACAATGAGCT GCAAGCTTTG AAGCTACAGC ATGAGCAAAA GATGTCTGAG 
TTAGAGAAGG CTCAGGTTGA AGTGCTGGAG GAGAAACTGG AGTTGGAGAA TTTGCAGCAG 
ACAGCCCTGC GACAGAAAGG GGAGATCGAG TGGCAGAAGC AGCTCTTAGA GAGGGACAAG 
CGAGAGATTG AGCGGATAAC TGCTGAGACC CGAGCATTGC AGTTGTGTGT TGAGTCTTTG 
TGCAAACAAA AGGAAGATCT TGAGGAGAAA TGTGACAGCT GGGAGAAGAA GCTGGCACAG 
ACCAAACGGG TTTTAGCAGC CACAGAAGAG AATAGCAAAG CAAAGCAATC AGACTTAGAA 
AAGTTGGAAT TGGATATCAG AAAACTGGAA CAGGAACTAG ACCAGCTAAA CAGAAACAAA 
CTCTCACTGC ACAGAGACAT CGCGGCCATG CAGCAGCAGG TCCAAGAAAC AGAAGTGAGA 
AATTGTCCTA ATATTAGAAA AACGAGAAGC AGTAAACTCA TTACAGGAGG AACTAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTRL ( XM_025282353.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025138138.1 Bubalus bubalis centriolin (CNTRL), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBu63440
Clone ID Related Accession (Same CDS sequence)	XM_025282353.1
Accession Version	XM_025282353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	centriolin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGATGG AAAAGCAAAG AGGAGAAATT GCTGAAAAGC AGGAGGAAAT CAAGGACCTC 
CAAAGAGTTA TTGATAGTCT GGATTCCAAA GACCCAAAAC ATTGCCATAT GAAGGCTCAG 
AAAAGGGGTA AAGAACAACA ATTTGACATT ATGAACAAGC AGTACAAACA ACTTGAGAGC 
CGTTTGGACG AGATACTTTC TAGAATTGCT AAAGAAACTG AAGAGATTAA GGACCTTGAA 
CAACAGCTTA CTGAAGGCCA GATAGCAGCA AATGAAGCCC TGAAGAAGGA CTTAGAAGGC 
GTCATCAGTG GCCTGCAAGA ATACCTGGGC ACTGTGAAGG GCCAGGCCGC TCAGGCCCAG 
AGCGAGTGCA GAAGGCTGCA GGATGAGAAA GAGGCGCTGC TGCAGAGGTT GGCCGAGGTC 
GAGCAGGAAA GGGACCAGCT GGAAGCAGTC GCCTTAGATG CAGAAGCTAT GAGGAAGGAG 
CTTGCAGAGC TAGAAAGCGC CCTCCAAGAG CAGCATGAGG TGAACGCGTC CCTGCAGCAG 
ACCCACGGAG ACCTCAGTGC CTACGAGGCT GAGCTAGAGG CACAGCTGAG GATGAGGGAT 
GCCGAAGCCA GCCAGCTCAA AGAAGAGCTG GGAGAACTAA CAAGGCTCAG CCAGCTAGAA 
CAGTCAACTC TACAAACAGA ACTTGAGAAA GAAAAACAAG CCCTCAAGAA CGCCCTGGGA 
AAAGCCCAGC TCTCGGAAGA AAAAGAACAA GAAAACAGTG AGCTCCGCAC CCAGCTTAAA 
CAGCTACAGG ATGACAATAA CCTGTTAAAA CAGCAACTTA AAGATTTCCA GAATCACCTT 
AATCAGGTAG CTGATGGTCT GATTCGTCCA GAAGAAGTGG CAGCTCGTGT AGATGAAGTA 
AGGAGAAAAT TGAAATCAGG CGCTGGAGAG ATGAGCAGAA TCCACAGTCC TTCAGATGTC 
TTAGGGAAAA GTCTTGCTGA CTTGCAGATA CAATTCAGTG AAATGCTGAC ACGTTCTCAG 
TGGGAGAAAG AGGAAGCCCA AGGGAGGGAA AGAAAACTCC AAGAAGAAAT GGCTCTGCAT 
CAGGAGAAGC TGGTGGATGG ACAGGAAGCG TTCAGGCAGG CCTGTGAGAG AGCGCTAGAA 
GCAAGGATTA ATTTTGACAA GAGGCAACAT GAAGCAAGAA TTCAGCAGTT AGAGAATGAG 
ATTCACTATT TACAAGAAAA TCTAAAAAGT ATGGAGGAAA TCCAAGGGCT TACAGATCTG 
CAGCTGCAGG AAGCTGATGA AGAGAAGGAG AGAATTCTAG CCCAACTCCA GGAGTTAGAG 
AAGAAGAAGA AACTTGAAGA TGCCAAATCT CAGGCACAGG TTCTAGGTTT AGATAAAGAA 
TTGAAGAAAC TAAAGAAGGC TGTGGCCACC TCTGATAAGA TAGCCACAGC TGAGCTCACC 
ATCGCCAAAG ACCAGCTCAA GTCCCTTCAT GGAACTGTTA TGAAAATTAA CCAGGAGCGT 
GCAGAGGACC TGCAGGAAGC AGAGCGGTTC AGCAGAAAGG CAGCACAGGC CGCGAGGGAT 
CTGACCCGAG CGGAAGCGGA GATCGAACTC CTGCAGAACC TCCTCAGGGA GAGAGAGGAG 
CAGATGGAGA AGGTAGAGGC AGGCACCCGA GGAGCAAGCT CACAGGCGCT GGAAATCGAG 
AGGTGGAATG AGACCATGGA GAGACAGAGG AGCGAGATCG CAAGGCTACG GGACTCACTA 
GACCGCACCG GGGCGGATAA CAGAGGAGGC ATTGAAAATA TTTTGGAAGA AATAGCTGAA 
CTCCGACGTG AAGTTTCTTA CCAGAATGAT TACATCAGCA GCATGGCAGA TCCTTTCAAA 
AGACGAGGCT ATTGGTACTT TATGCCACCA CCATCATCAT CAAAAGTTTC CAGCCACAGC 
TCCCAGGCCA CAAAGGACTC TGGTGTCGGG ATGAAGTACG CAGCCTCGAC TCCTATTAGA 
AAACCACAGC AGAACGGGAG GGAAGACGGT GCCCCTCCAG CTGCCTCCGG ATACTGGGTG 
TATTCCCCCA TCAGGAGCGG CTTACATAAA TCGTTTTCAA ATAAAGATGC AGACAGTGGA 
GGAGAGAGCC GGGAGGAGAG CGAGCTGGAT GACCACGATG ACCGCCCGTT CGTCCCTCCT 
CCTGGATACA TGATGTACAC CGTGTTCCCC GACGGCTCTC CTGTACCACA GGGCATGGCC 
CTCTACGCAC CGCCGCCTCC CTTGCCCAAC AATAGCCGAC CTCTCAGCCC TGGCACTGTT 
GTGTATGGCC CGCCCCCTGC TGGTGCCCCC ATTGTCTACG GGCCCCCACC GCCCAGCTTC 
TCCGTCCCCC TCATCCCAGT GGGCGTGCTG CACTGCAACG TCCCGGAGCA CCATGCCTTA 
GAGAATGAGG TTTCTAGATT GGAAGACATA ATACAGCATT TAAAATCAAA GAAGCAGGAA 
CAGAGGATGA GAGTATGCAA GCGGCAGTCT GAGAAAGAAA TAGAAGAACT GCATCATAAT 
ATTGATGAAC TTTTGCAAGA GAAGAAAGAC TTAGAGCACG AAGTAGAAGA ATTACATAGA 
ACCATCCAGA AACATCAACA GCAAAAAGAC TTCATTGATG GAAATGTTGA GAGTCTTATG 
AATGAACTAA AAATAGAAAA ATCACTCAAG CACCATGAAG ATATTGTAGA TGAAATTGAG 
TGCATCGAGA AGACTCTCCT GAAACGTCGA GCAGAGCTCA GGGAGGCTGA CCGACTGCTG 
GCGGAAGCCG AGAGTGAACT TTCACGCACA ACAGAGAAAA CAAAAAATGC TGTTGAAAAG 
TTCACTGATG CCAAGAGGAA TTTGTTGCAA ACTGAGAAAG ATGCTGAGGA GTTAGAAAGG 
AGAGCTCAGG AAACAGCTGT TAGCCTTGTC AAGGCTGATC AACAGCTCAG ATTGTTACAG 
GCGGACACAA AGGATTTGGA GCAGCACAAA ACTGAGCAAG AAGAGATCTT GAAAGAGATA 
AACAAAGTTG TTGCAGCAAA AGACTCAGAC TTCCAGTGTT TAAACACGAA GAAGGAGAAG 
CTAACAGAAG AGCTGCAGAA GCTGCAGAAA GACATCGAGA CGGCAGCGCG CACGGAGGAT 
CACCACCTGC AGGTCCTGAG AGAGTCGGAG GCCCTCCTGC AGGCCAAGAG AGCGGAGCTG 
GACAAGCTGA GGAGCCAGGT AACGAGTCAG CAGCAGGAGA TGGCTGTCTT GGACAGACAG 
CTGGGGCATA AGAAGGAGGA GCTGCATCTA CTCCAGGGAA GCATGGTCCA GGCCAGAGCT 
GACCTCCAGG AAGCGCTGAG GCTGGGAGAA ACTGAAGTAA CTGAGAAGTG CAACCACATC 
AGGGAACTAA AAGGTCTTCT GGAAGAACTG AGTTTTCAGA AAGGAGAACT GAATGTCCAG 
ATTAGTGAGA AAAAAAATCA ACTTTCACTT ATAAAGCAAG AAATTGAAAA AGAGGAAGAA 
AATCTTCAGG TAGTTTTAGG GCAGATGTCT AAACATAAAA CTGAACTTAA GAATATTCTG 
GACATGTTGC AACTTGAAAA CAATGAGCTG CAAGCTTTGA AGCTACAGCA TGAGCAAAAG 
ATGTCTGAGT TAGAGAAGGC TCAGGTTGAA GTGCTGGAGG AGAAACTGGA GTTGGAGAAT 
TTGCAGCAGA CAGCCCTGCG ACAGAAAGGG GAGATCGAGT GGCAGAAGCA GCTCTTAGAG 
AGGGACAAGC GAGAGATTGA GCGGATAACT GCTGAGACCC GAGCATTGCA GTTGTGTGTT 
GAGTCTTTGT GCAAACAAAA GGAAGATCTT GAGGAGAAAT GTGACAGCTG GGAGAAGAAG 
CTGGCACAGA CCAAACGGGT TTTAGCAGCC ACAGAAGAGA ATAGCAAAGC AAAGCAATCA 
GACTTAGAAA AGTTGGAATT GGATATCAGA AAACTGGAAC AGGAACTAGA CCAGCTAAAC 
AGAAACAAAC TCTCACTGCA CAGAGACATC GCGGCCATGC AGCAGCAGGT CCAAGAAAAA 
CGAGAAGCAG TAAACTCATT ACAGGAGGAA CTAGCTGATG TCCAAGCCCA TTTGAACCTA 
GCGAAACAGG ACCTGCTCCA CACCACCAAG CGCCAGGATG TCCTGCTTGG CGAGCAGACC 
AGGCTGCAGG AGGACCTCCA GGAACGCAGA AAGAAGTTGG AAGTCTGCCA AAAAGAAGAG 
GCAACAAAGC GGCAGCACCT GCAAGCGCTT CAGACGGAGA TGGAGGCGAA CGAGGCCGAG 
CTGGCCCGGC AGGAGACGGT ACCGCACCCG CGGGCACGCG CGGGGGGGCA CTGCCACGCG 
CAGAGCGCTC TTCCCCTGGG GTCTGAGGTG GAGGCGAACG AGAGCGAGCT GGCCCGGCAG 
GAGACGACAT TTCAGAGACT CCAGAAAGAG AGAGAAAGTG AAGAAAAAAA GTTAGAAGCC 
AGTCGAGCGA CTCTGCAGGA GCAGCAGCAG CAGCTGGAAA AGGAAATCGC AGACCAGAAG 
AACGTGCTGG ACCAGGTGCT CAGCCAGGTG CTGGGGGCCA AGGAGCGCGC CAGGACTCTG 
CAGCAGGAGG AGAGGTGGGG TGAGACCCTG GAGAAGACGC TCTCCCAGAC CCAGCAACAG 
CTCTCAGAAC GGGAGCAGCA GTTGTTGGAA AAGTCAGCTG AGCTGCTGGC TCTTCAGAAA 
GAGGCAGACT CGATGAGGGC AGACTTCAGC CTCTTGCGAA ACCAGTTCTT GACGGAGAGG 
AAGAAAGCCG AGAAGCAGGT AGCCAGCCTC AGGGAAGCGC TCAAGACCCA GCGCAGCCAG 
CTGGAGAAGA CCCTTCTCGA GCAGAAGCAG GAGAACAGCT CCATGCAGAA GGAGATGGCG 
ACTATCGAGC AGGTGGCGCA GGACAACCAC GAGCGGGCCC GGCGCCTGAT GCGGGAGCTC 
AGCCAGATGC AGCAGGAGTA CGCGGAGCTC AAGAAGCAGA TGGTAAACCA GAAAGATCTG 
GAAAGAAGAC AAGTAGAAAT CAGTGAAGCG ATGAGGACAC TTAAGTCTGA AGTGAAAGAC 
GAGATCAGAG CCAGCCTGAA GAACCTCAAC CAGTTTCTCC CGGAGCTGCC AGCAGACCTG 
GAGGCCATTC TGGAAAGGAG CGAACACCTA GACGGAGGGC TGGAGAGCTT GAAGGAGAAC 
TTTCCATTCG CCCTGAGTGA GAAGCCATCA CCTTTGGAAG AGAAACTACA TTTTTCTCGC 
GTTCACATAA TGGATGAGCA CTGGCGTGGA GAAGCGCTCC GGGAGAAGCT GCGGCACCGT 
GAGGACCGAC TCAAGGCCCA GCTCCGACAC TGTATGTCCA AGCAGGCAGA GGTGTTAAGC 
AAAGGGAAGC GGCAGACCGA GGGCACGTTA CACAGCCTGA GGAGGCAGGT CGACGCCTTA 
GGGGAGCTGG TCACCAGCAC GTCTGTGGAG TCCACGTCGT CAGCCACTCT GTCTCAGCTC 
GAATCTTCCC TTGCAGAGGA CTCCCATCTT GGACCAAGTC AGAGCTCCTT CCATCAAGTT 
CCACAAGGTC CATCACAATC AGTAGACAGT TACCGACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025138138.1
CDS	546..6107
Translation	MQMEKQRGEIAEKQEEIKDLQRVIDSLDSKDPKHCHMKAQKRGKEQQFDIMNKQYKQLESRLDEILSRIAKETEEIKDLEQQLTEGQIAANEALKKDLEGVISGLQEYLGTVKGQAAQAQSECRRLQDEKEALLQRLAEVEQERDQLEAVALDAEAMRKELAELESALQEQHEVNASLQQTHGDLSAYEAELEAQLRMRDAEASQLKEELGELTRLSQLEQSTLQTELEKEKQALKNALGKAQLSEEKEQENSELRTQLKQLQDDNNLLKQQLKDFQNHLNQVADGLIRPEEVAARVDEVRRKLKSGAGEMSRIHSPSDVLGKSLADLQIQFSEMLTRSQWEKEEAQGRERKLQEEMALHQEKLVDGQEAFRQACERALEARINFDKRQHEARIQQLENEIHYLQENLKSMEEIQGLTDLQLQEADEEKERILAQLQELEKKKKLEDAKSQAQVLGLDKELKKLKKAVATSDKIATAELTIAKDQLKSLHGTVMKINQERAEDLQEAERFSRKAAQAARDLTRAEAEIELLQNLLREREEQMEKVEAGTRGASSQALEIERWNETMERQRSEIARLRDSLDRTGADNRGGIENILEEIAELRREVSYQNDYISSMADPFKRRGYWYFMPPPSSSKVSSHSSQATKDSGVGMKYAASTPIRKPQQNGREDGAPPAASGYWVYSPIRSGLHKSFSNKDADSGGESREESELDDHDDRPFVPPPGYMMYTVFPDGSPVPQGMALYAPPPPLPNNSRPLSPGTVVYGPPPAGAPIVYGPPPPSFSVPLIPVGVLHCNVPEHHALENEVSRLEDIIQHLKSKKQEQRMRVCKRQSEKEIEELHHNIDELLQEKKDLEHEVEELHRTIQKHQQQKDFIDGNVESLMNELKIEKSLKHHEDIVDEIECIEKTLLKRRAELREADRLLAEAESELSRTTEKTKNAVEKFTDAKRNLLQTEKDAEELERRAQETAVSLVKADQQLRLLQADTKDLEQHKTEQEEILKEINKVVAAKDSDFQCLNTKKEKLTEELQKLQKDIETAARTEDHHLQVLRESEALLQAKRAELDKLRSQVTSQQQEMAVLDRQLGHKKEELHLLQGSMVQARADLQEALRLGETEVTEKCNHIRELKGLLEELSFQKGELNVQISEKKNQLSLIKQEIEKEEENLQVVLGQMSKHKTELKNILDMLQLENNELQALKLQHEQKMSELEKAQVEVLEEKLELENLQQTALRQKGEIEWQKQLLERDKREIERITAETRALQLCVESLCKQKEDLEEKCDSWEKKLAQTKRVLAATEENSKAKQSDLEKLELDIRKLEQELDQLNRNKLSLHRDIAAMQQQVQEKREAVNSLQEELADVQAHLNLAKQDLLHTTKRQDVLLGEQTRLQEDLQERRKKLEVCQKEEATKRQHLQALQTEMEANEAELARQETVPHPRARAGGHCHAQSALPLGSEVEANESELARQETTFQRLQKERESEEKKLEASRATLQEQQQQLEKEIADQKNVLDQVLSQVLGAKERARTLQQEERWGETLEKTLSQTQQQLSEREQQLLEKSAELLALQKEADSMRADFSLLRNQFLTERKKAEKQVASLREALKTQRSQLEKTLLEQKQENSSMQKEMATIEQVAQDNHERARRLMRELSQMQQEYAELKKQMVNQKDLERRQVEISEAMRTLKSEVKDEIRASLKNLNQFLPELPADLEAILERSEHLDGGLESLKENFPFALSEKPSPLEEKLHFSRVHIMDEHWRGEALREKLRHREDRLKAQLRHCMSKQAEVLSKGKRQTEGTLHSLRRQVDALGELVTSTSVESTSSATLSQLESSLAEDSHLGPSQSSFHQVPQGPSQSVDSYRH

Target ORF information:

RefSeq Version	XM_025282353.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centriolin (CNTRL), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025282353.1

ORF Insert Sequence:

ATGCAGATGG AAAAGCAAAG AGGAGAAATT GCTGAAAAGC AGGAGGAAAT CAAGGACCTC 
CAAAGAGTTA TTGATAGTCT GGATTCCAAA GACCCAAAAC ATTGCCATAT GAAGGCTCAG 
AAAAGGGGTA AAGAACAACA ATTTGACATT ATGAACAAGC AGTACAAACA ACTTGAGAGC 
CGTTTGGACG AGATACTTTC TAGAATTGCT AAAGAAACTG AAGAGATTAA GGACCTTGAA 
CAACAGCTTA CTGAAGGCCA GATAGCAGCA AATGAAGCCC TGAAGAAGGA CTTAGAAGGC 
GTCATCAGTG GCCTGCAAGA ATACCTGGGC ACTGTGAAGG GCCAGGCCGC TCAGGCCCAG 
AGCGAGTGCA GAAGGCTGCA GGATGAGAAA GAGGCGCTGC TGCAGAGGTT GGCCGAGGTC 
GAGCAGGAAA GGGACCAGCT GGAAGCAGTC GCCTTAGATG CAGAAGCTAT GAGGAAGGAG 
CTTGCAGAGC TAGAAAGCGC CCTCCAAGAG CAGCATGAGG TGAACGCGTC CCTGCAGCAG 
ACCCACGGAG ACCTCAGTGC CTACGAGGCT GAGCTAGAGG CACAGCTGAG GATGAGGGAT 
GCCGAAGCCA GCCAGCTCAA AGAAGAGCTG GGAGAACTAA CAAGGCTCAG CCAGCTAGAA 
CAGTCAACTC TACAAACAGA ACTTGAGAAA GAAAAACAAG CCCTCAAGAA CGCCCTGGGA 
AAAGCCCAGC TCTCGGAAGA AAAAGAACAA GAAAACAGTG AGCTCCGCAC CCAGCTTAAA 
CAGCTACAGG ATGACAATAA CCTGTTAAAA CAGCAACTTA AAGATTTCCA GAATCACCTT 
AATCAGGTAG CTGATGGTCT GATTCGTCCA GAAGAAGTGG CAGCTCGTGT AGATGAAGTA 
AGGAGAAAAT TGAAATCAGG CGCTGGAGAG ATGAGCAGAA TCCACAGTCC TTCAGATGTC 
TTAGGGAAAA GTCTTGCTGA CTTGCAGATA CAATTCAGTG AAATGCTGAC ACGTTCTCAG 
TGGGAGAAAG AGGAAGCCCA AGGGAGGGAA AGAAAACTCC AAGAAGAAAT GGCTCTGCAT 
CAGGAGAAGC TGGTGGATGG ACAGGAAGCG TTCAGGCAGG CCTGTGAGAG AGCGCTAGAA 
GCAAGGATTA ATTTTGACAA GAGGCAACAT GAAGCAAGAA TTCAGCAGTT AGAGAATGAG 
ATTCACTATT TACAAGAAAA TCTAAAAAGT ATGGAGGAAA TCCAAGGGCT TACAGATCTG 
CAGCTGCAGG AAGCTGATGA AGAGAAGGAG AGAATTCTAG CCCAACTCCA GGAGTTAGAG 
AAGAAGAAGA AACTTGAAGA TGCCAAATCT CAGGCACAGG TTCTAGGTTT AGATAAAGAA 
TTGAAGAAAC TAAAGAAGGC TGTGGCCACC TCTGATAAGA TAGCCACAGC TGAGCTCACC 
ATCGCCAAAG ACCAGCTCAA GTCCCTTCAT GGAACTGTTA TGAAAATTAA CCAGGAGCGT 
GCAGAGGACC TGCAGGAAGC AGAGCGGTTC AGCAGAAAGG CAGCACAGGC CGCGAGGGAT 
CTGACCCGAG CGGAAGCGGA GATCGAACTC CTGCAGAACC TCCTCAGGGA GAGAGAGGAG 
CAGATGGAGA AGGTAGAGGC AGGCACCCGA GGAGCAAGCT CACAGGCGCT GGAAATCGAG 
AGGTGGAATG AGACCATGGA GAGACAGAGG AGCGAGATCG CAAGGCTACG GGACTCACTA 
GACCGCACCG GGGCGGATAA CAGAGGAGGC ATTGAAAATA TTTTGGAAGA AATAGCTGAA 
CTCCGACGTG AAGTTTCTTA CCAGAATGAT TACATCAGCA GCATGGCAGA TCCTTTCAAA 
AGACGAGGCT ATTGGTACTT TATGCCACCA CCATCATCAT CAAAAGTTTC CAGCCACAGC 
TCCCAGGCCA CAAAGGACTC TGGTGTCGGG ATGAAGTACG CAGCCTCGAC TCCTATTAGA 
AAACCACAGC AGAACGGGAG GGAAGACGGT GCCCCTCCAG CTGCCTCCGG ATACTGGGTG 
TATTCCCCCA TCAGGAGCGG CTTACATAAA TCGTTTTCAA ATAAAGATGC AGACAGTGGA 
GGAGAGAGCC GGGAGGAGAG CGAGCTGGAT GACCACGATG ACCGCCCGTT CGTCCCTCCT 
CCTGGATACA TGATGTACAC CGTGTTCCCC GACGGCTCTC CTGTACCACA GGGCATGGCC 
CTCTACGCAC CGCCGCCTCC CTTGCCCAAC AATAGCCGAC CTCTCAGCCC TGGCACTGTT 
GTGTATGGCC CGCCCCCTGC TGGTGCCCCC ATTGTCTACG GGCCCCCACC GCCCAGCTTC 
TCCGTCCCCC TCATCCCAGT GGGCGTGCTG CACTGCAACG TCCCGGAGCA CCATGCCTTA 
GAGAATGAGG TTTCTAGATT GGAAGACATA ATACAGCATT TAAAATCAAA GAAGCAGGAA 
CAGAGGATGA GAGTATGCAA GCGGCAGTCT GAGAAAGAAA TAGAAGAACT GCATCATAAT 
ATTGATGAAC TTTTGCAAGA GAAGAAAGAC TTAGAGCACG AAGTAGAAGA ATTACATAGA 
ACCATCCAGA AACATCAACA GCAAAAAGAC TTCATTGATG GAAATGTTGA GAGTCTTATG 
AATGAACTAA AAATAGAAAA ATCACTCAAG CACCATGAAG ATATTGTAGA TGAAATTGAG 
TGCATCGAGA AGACTCTCCT GAAACGTCGA GCAGAGCTCA GGGAGGCTGA CCGACTGCTG 
GCGGAAGCCG AGAGTGAACT TTCACGCACA ACAGAGAAAA CAAAAAATGC TGTTGAAAAG 
TTCACTGATG CCAAGAGGAA TTTGTTGCAA ACTGAGAAAG ATGCTGAGGA GTTAGAAAGG 
AGAGCTCAGG AAACAGCTGT TAGCCTTGTC AAGGCTGATC AACAGCTCAG ATTGTTACAG 
GCGGACACAA AGGATTTGGA GCAGCACAAA ACTGAGCAAG AAGAGATCTT GAAAGAGATA 
AACAAAGTTG TTGCAGCAAA AGACTCAGAC TTCCAGTGTT TAAACACGAA GAAGGAGAAG 
CTAACAGAAG AGCTGCAGAA GCTGCAGAAA GACATCGAGA CGGCAGCGCG CACGGAGGAT 
CACCACCTGC AGGTCCTGAG AGAGTCGGAG GCCCTCCTGC AGGCCAAGAG AGCGGAGCTG 
GACAAGCTGA GGAGCCAGGT AACGAGTCAG CAGCAGGAGA TGGCTGTCTT GGACAGACAG 
CTGGGGCATA AGAAGGAGGA GCTGCATCTA CTCCAGGGAA GCATGGTCCA GGCCAGAGCT 
GACCTCCAGG AAGCGCTGAG GCTGGGAGAA ACTGAAGTAA CTGAGAAGTG CAACCACATC 
AGGGAACTAA AAGGTCTTCT GGAAGAACTG AGTTTTCAGA AAGGAGAACT GAATGTCCAG 
ATTAGTGAGA AAAAAAATCA ACTTTCACTT ATAAAGCAAG AAATTGAAAA AGAGGAAGAA 
AATCTTCAGG TAGTTTTAGG GCAGATGTCT AAACATAAAA CTGAACTTAA GAATATTCTG 
GACATGTTGC AACTTGAAAA CAATGAGCTG CAAGCTTTGA AGCTACAGCA TGAGCAAAAG 
ATGTCTGAGT TAGAGAAGGC TCAGGTTGAA GTGCTGGAGG AGAAACTGGA GTTGGAGAAT 
TTGCAGCAGA CAGCCCTGCG ACAGAAAGGG GAGATCGAGT GGCAGAAGCA GCTCTTAGAG 
AGGGACAAGC GAGAGATTGA GCGGATAACT GCTGAGACCC GAGCATTGCA GTTGTGTGTT 
GAGTCTTTGT GCAAACAAAA GGAAGATCTT GAGGAGAAAT GTGACAGCTG GGAGAAGAAG 
CTGGCACAGA CCAAACGGGT TTTAGCAGCC ACAGAAGAGA ATAGCAAAGC AAAGCAATCA 
GACTTAGAAA AGTTGGAATT GGATATCAGA AAACTGGAAC AGGAACTAGA CCAGCTAAAC 
AGAAACAAAC TCTCACTGCA CAGAGACATC GCGGCCATGC AGCAGCAGGT CCAAGAAAAA 
CGAGAAGCAG TAAACTCATT ACAGGAGGAA CTAGCTGATG TCCAAGCCCA TTTGAACCTA 
GCGAAACAGG ACCTGCTCCA CACCACCAAG CGCCAGGATG TCCTGCTTGG CGAGCAGACC 
AGGCTGCAGG AGGACCTCCA GGAACGCAGA AAGAAGTTGG AAGTCTGCCA AAAAGAAGAG 
GCAACAAAGC GGCAGCACCT GCAAGCGCTT CAGACGGAGA TGGAGGCGAA CGAGGCCGAG 
CTGGCCCGGC AGGAGACGGT ACCGCACCCG CGGGCACGCG CGGGGGGGCA CTGCCACGCG 
CAGAGCGCTC TTCCCCTGGG GTCTGAGGTG GAGGCGAACG AGAGCGAGCT GGCCCGGCAG 
GAGACGACAT TTCAGAGACT CCAGAAAGAG AGAGAAAGTG AAGAAAAAAA GTTAGAAGCC 
AGTCGAGCGA CTCTGCAGGA GCAGCAGCAG CAGCTGGAAA AGGAAATCGC AGACCAGAAG 
AACGTGCTGG ACCAGGTGCT CAGCCAGGTG CTGGGGGCCA AGGAGCGCGC CAGGACTCTG 
CAGCAGGAGG AGAGGTGGGG TGAGACCCTG GAGAAGACGC TCTCCCAGAC CCAGCAACAG 
CTCTCAGAAC GGGAGCAGCA GTTGTTGGAA AAGTCAGCTG AGCTGCTGGC TCTTCAGAAA 
GAGGCAGACT CGATGAGGGC AGACTTCAGC CTCTTGCGAA ACCAGTTCTT GACGGAGAGG 
AAGAAAGCCG AGAAGCAGGT AGCCAGCCTC AGGGAAGCGC TCAAGACCCA GCGCAGCCAG 
CTGGAGAAGA CCCTTCTCGA GCAGAAGCAG GAGAACAGCT CCATGCAGAA GGAGATGGCG 
ACTATCGAGC AGGTGGCGCA GGACAACCAC GAGCGGGCCC GGCGCCTGAT GCGGGAGCTC 
AGCCAGATGC AGCAGGAGTA CGCGGAGCTC AAGAAGCAGA TGGTAAACCA GAAAGATCTG 
GAAAGAAGAC AAGTAGAAAT CAGTGAAGCG ATGAGGACAC TTAAGTCTGA AGTGAAAGAC 
GAGATCAGAG CCAGCCTGAA GAACCTCAAC CAGTTTCTCC CGGAGCTGCC AGCAGACCTG 
GAGGCCATTC TGGAAAGGAG CGAACACCTA GACGGAGGGC TGGAGAGCTT GAAGGAGAAC 
TTTCCATTCG CCCTGAGTGA GAAGCCATCA CCTTTGGAAG AGAAACTACA TTTTTCTCGC 
GTTCACATAA TGGATGAGCA CTGGCGTGGA GAAGCGCTCC GGGAGAAGCT GCGGCACCGT 
GAGGACCGAC TCAAGGCCCA GCTCCGACAC TGTATGTCCA AGCAGGCAGA GGTGTTAAGC 
AAAGGGAAGC GGCAGACCGA GGGCACGTTA CACAGCCTGA GGAGGCAGGT CGACGCCTTA 
GGGGAGCTGG TCACCAGCAC GTCTGTGGAG TCCACGTCGT CAGCCACTCT GTCTCAGCTC 
GAATCTTCCC TTGCAGAGGA CTCCCATCTT GGACCAAGTC AGAGCTCCTT CCATCAAGTT 
CCACAAGGTC CATCACAATC AGTAGACAGT TACCGACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTRL ( XM_006054143.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006054205.1 Bubalus bubalis centriolin (CNTRL), transcript variant X1, mRNA.

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CloneID	OBu11628
Clone ID Related Accession (Same CDS sequence)	XM_006054143.1
Accession Version	XM_006054143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	centriolin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784589.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCTAAGACA GAAGACCATG 
GAACTAACGC GAGCATGTCA AAAACAGTAT GAACTGGAAC AGGAGTTGGC TTTTTACAAA 
ATCGATGCTA AATTTGAGCC ACTGAATTAT TATCCCTCAG AGTATGTTGA CGTTGATAAA 
GCCCCAGATG AAAGCCCTTA CATTGGCAAG TCCAGATACA AGAGAAATAT GTTCGCCACA 
GAGAGTTACA TTGTGAATAA TGCCCAGCCA GTTGAGATCA AGAGGATGGA GCCAGAGGAA 
GGGGAGCAAC TTAGAAACGA GCATGTGAGC TTGAAAGCCC ATATGCCACT GGACACGGAG 
CTGGAAGACA AAGAGAACAA AATAAGTACA GCACAAGCTC GACTATCAGC ACTGCATGAT 
GAAATAGAAA AGGCAGAACA ACAAATTTTA AGAGCCACTG AAGAATTTAA ACAACTGGAA 
GAAGCTGTAC AACTTAAAAA GATTTCAGAA GCAGAGAAAG ACTTTCTTCT CAAGCAGCTG 
AGTGGCAGGA TACAGCTTCT AAATAAATTA CGCCAGGAAG CTCTTGATCT AGAAATGCAG 
ATGGAAAAGC AAAGAGGAGA AATTGCTGAA AAGCAGGAGG AAATCAAGGA CCTCCAAAGA 
GTTATTGATA GTCTGGATTC CAAAGACCCA AAACATTGCC ATATGAAGGC TCAGAAAAGG 
GGTAAAGAAC AACAATTTGA CATTATGAAC AAGCAGTACA AACAACTTGA GAGCCGTTTG 
GACGAGATAC TTTCTAGAAT TGCTAAAGAA ACTGAAGAGA TTAAGGACCT TGAACAACAG 
CTTACTGAAG GCCAGATAGC AGCAAATGAA GCCCTGAAGA AGGACTTAGA AGGCGTCATC 
AGTGGCCTGC AAGAATACCT GGGCACTGTG AAGGGCCAGG CCGCTCAGGC CCAGAGCGAG 
TGCAGAAGGC TGCAGGATGA GAAAGAGGCG CTGCTGCAGA GGTTGGCCGA GGTCGAGCAG 
GAAAGGGACC AGCTGGAAGC AGTCGCCTTA GATGCAGAAG CTATGAGGAA GGAGCTTGCA 
GAGCTAGAAA GCGCCCTCCA AGAGCAGCAT GAGGTGAACG CGTCCCTGCA GCAGACCCAC 
GGAGACCTCA GTGCCTACGA GGCTGAGCTA GAGGCACAGC TGAGGATGAG GGATGCCGAA 
GCCAGCCAGC TCAAAGAAGA GCTGGGAGAA CTAACAAGGC TCAGCCAGCT AGAACAGTCA 
ACTCTACAAA CAGAACTTGA GAAAGAAAAA CAAGCCCTCA AGAACGCCCT GGGAAAAGCC 
CAGCTCTCGG AAGAAAAAGA ACAAGAAAAC AGTGAGCTCC GCACCCAGCT TAAACAGCTA 
CAGGATGACA ATAACCTGTT AAAACAGCAA CTTAAAGATT TCCAGAATCA CCTTAATCAG 
GTAGCTGATG GTCTGATTCG TCCAGAAGAA GTGGCAGCTC GTGTAGATGA AGTAAGGAGA 
AAATTGAAAT CAGGCGCTGG AGAGATGAGC AGAATCCACA GTCCTTCAGA TGTCTTAGGG 
AAAAGTCTTG CTGACTTGCA GATACAATTC AGTGAAATGC TGACACGTTC TCAGTGGGAG 
AAAGAGGAAG CCCAAGGGAG GGAAAGAAAA CTCCAAGAAG AAATGGCTCT GCATCAGGAG 
AAGCTGGTGG ATGGACAGGA AGCGTTCAGG CAGGCCTGTG AGAGAGCGCT AGAAGCAAGG 
ATTAATTTTG ACAAGAGGCA ACATGAAGCA AGAATTCAGC AGTTAGAGAA TGAGATTCAC 
TATTTACAAG AAAATCTAAA AAGTATGGAG GAAATCCAAG GGCTTACAGA TCTGCAGCTG 
CAGGAAGCTG ATGAAGAGAA GGAGAGAATT CTAGCCCAAC TCCAGGAGTT AGAGAAGAAG 
AAGAAACTTG AAGATGCCAA ATCTCAGGCA CAGGTTCTAG GTTTAGATAA AGAATTGAAG 
AAACTAAAGA AGGCTGTGGC CACCTCTGAT AAGATAGCCA CAGCTGAGCT CACCATCGCC 
AAAGACCAGC TCAAGTCCCT TCATGGAACT GTTATGAAAA TTAACCAGGA GCGTGCAGAG 
GACCTGCAGG AAGCAGAGCG GTTCAGCAGA AAGGCAGCAC AGGCCGCGAG GGATCTGACC 
CGAGCGGAAG CGGAGATCGA ACTCCTGCAG AACCTCCTCA GGGAAAGAGA GGAGCAGATG 
GAGAAGGTAG AGGCAGGCAC CCGAGGAGCA AGCTCACAGG CGCTGGAAAT CGAGAGGTGG 
AATGAGACCA TGGAGAGACA GAGGAGCGAG ATCGCAAGGC TACGGGACTC ACTAGACCGC 
ACCGGGGCGG ATAACAGAGG AGGCATTGAA AATATTTTGG AAGAAATAGC TGAACTCCGA 
CGTGAAGTTT CTTACCAGAA TGATTACATC AGCAGCATGG CAGATCCTTT CAAAAGACGA 
GGCTATTGGT ACTTTATGCC ACCACCATCA TCATCAAAAG TTTCCAGCCA CAGCTCCCAG 
GCCACAAAGG ACTCTGGTGT CGGGATGAAG TACGCAGCCT CGACTCCTAT TAGAAAACCA 
CAGCAGAACG GGAGGGAAGA CGGTGCCCCT CCAGCTGCCT CCGGATACTG GGTGTATTCC 
CCCATCAGGA GCGGCTTACA TAAATCGTTT TCAAATAAAG ATGCAGACAG TGGAGGAGAG 
AGCCGGGAGG AGAGCGAGCT GGATGACCAC GATGACCGCC CGTTCGTCCC TCCTCCTGGA 
TACATGATGT ACACCGTGTT CCCCGACGGC TCTCCTGTAC CACAGGGCAT GGCCCTCTAC 
GCACCGCCGC CTCCCTTGCC CAACAATAGC CGACCTCTCA GCCCTGGCAC TGTTGTGTAT 
GGCCCGCCCC CTGCTGGTGC CCCCATTGTC TACGGGCCCC CACCGCCCAG CTTCTCCGTC 
CCCCTCATCC CAGTGGGCGT GCTGCACTGC AACGTCCCGG AGCACCATGC CTTAGAGAAT 
GAGGTTTCTA GATTGGAAGA CATAATACAG CATTTAAAAT CAAAGAAGCA GGAACAGAGG 
ATGAGAGTAT GCAAGCGGCA GTCTGAGAAA GAAATAGAAG AACTGCATCA TAATATTGAT 
GAACTTTTGC AAGAGAAGAA AGACTTAGAG CACGAAGTAG AAGAATTACA TAGAACCATC 
CAGAAACATC AACAGCGAAA AGACTTCATT GATGGAAATG TTGAGAGTCT TATGAATGAA 
CTAAAAATAG AAAAATCACT CAAGCACCAT GAAGATATTG TAGATGAAAT TGAGTGCATC 
GAGAAGACTC TCCTGAAACG TCGAGCAGAG CTCAGGGAGG CTGACCGACT GCTGGCGGAA 
GCCGAGAGTG AACTTTCACG CACAACAGAG AAAACAAAAA ATGCTGTTGA AAAGTTCACT 
GATGCCAAGA GGAATTTGTT GCAAACTGAG AAAGATGCTG AGGAGTTAGA AAGGAGAGCT 
CAGGAAACAG CTGTTAGCCT TGTCAAGGCT GATCAACAGC TCAGATTGTT ACAGGCGGAC 
ACAAAGGATT TGGAGCAGCA CAAAACTGAG CAAGAAGAGA TCTTGAAAGA GATAAACAAA 
GTTGTTGCAG CAAAAGACTC AGACTTCCAG TGTTTAAACA CGAAGAAGGA GAAGCTAACA 
GAAGAGCTGC AGAAGCTGCA GAAAGACATC GAGACGGCAG CGCGCACGGA GGATCACCAC 
CTGCAGGTCC TGAGAGAGTC GGAGGCCCTC CTGCAGGCCA AGAGAGCGGA GCTGGACAAG 
CTGAGGAGCC AGGTAACGAG TCAGCAGCAG GAGATGGCTG TCTTGGACAG ACAGCTGGGG 
CATAAGAAGG AGGAGCTGCA TCTACTCCAG GGAAGCATGG TCCAGGCCAG AGCTGACCTC 
CAGGAAGCGC TGAGGCTGGG AGAAACTGAA GTAACTGAGA AGTGCAACCA CATCAGGGAA 
CTAAAAGGTC TTCTGGAAGA ACTGAGTTTT CAGAAAGGAG AACTGAATGT CCAGATTAGT 
GAGAAAAAAA ATCAACTTTC ACTTATAAAG CAAGAAATTG AAAAAGAGGA AGAAAATCTT 
CAGGTAGTTT TAGGGCAGAT GTCTAAACAT AAAACTGAAC TTAAGAATAT TCTGGACATG 
TTGCAACTTG AAAACAATGA GCTGCAAGCT TTGAAGCTAC AGCATGAGCA AAAGATGTCT 
GAGTTAGAGA AGGCTCAGGT TGAAGTGCTG GAGGAGAAAC TGGAGTTGGA GAATTTGCAG 
CAGACAGCCC TGCGACAGAA AGGGGAGATC GAGTGGCAGA AGCAGCTCTT AGAGAGGGAC 
AAGCGAGAGA TTGAGCGGAT AACTGCTGAG ACCCGAGCAT TGCAGTTGTG TGTTGAGTCT 
TTGTGCAAAC AAAAGGAAGA TCTTGAGGAG AAATGTGACA GCTGGGAGAA GAAGCTGGCA 
CAGACCAAAC GGGTTTTAGC AGCCACAGAA GAGAATAGCA AAGCAAAGCA ATCAGACTTA 
GAAAAGTTGG AATTGGATAT CAGAAAACTG GAACAGGAAC TAGACCAGCT AAACAGAAAC 
AAACTCTCAC TGCACAGAGA CATCGCGGCC ATGCAGCAGC AGGTCCAAGA AAAACGAGAA 
GCAGTAAACT CATTACAGGA GGAACTAGCT GATGTCCAAG CCCATTTGAA CCTAGCGAAA 
CAGGACCTGC TCCACACCAC CAAGCGCCAG GATGTCCTGC TTGGCGAGCA GACCAGGCTG 
CAGGAGGACC TCCAGGAACG CAGAAAGAAG TTGGAAGTCT GCCAAAAAGA AGAGGCAACA 
AAGCGGCAGC ACCTGCAAGC GCTTCAGACG GAGATGGAGG CGAACGAGGC CGAGCTGGCC 
CGGCAGGAGA CGACATTTCA GAGACTCCAG AAAGAGAGAG AAAGTGAAGA AAAAAAGTTA 
GAAGCCAGTC GAGCGACTCT GCAGGAGCAG CAGCAGCAGC TGGAAAAGGA AATCGCAGAC 
CAGAAGAACG TGCTGGACCA GGTGCTCAGC CAGGTGCTGG GGGCCAAGGA GCGCGCCAGG 
ACTCTGCAGC AGGAGGAGAG GTGGGGTGAG ACCCTGGAGA AGACGCTCTC CCAGACCCAG 
CAACAGCTCT CAGAACGGGA GCAGCAGTTG TTGGAAAAGT CAGCTGAGCT GCTGGCTCTT 
CAGAAAGAGG CAGACTCGAT GAGGGCAGAC TTCAGCCTCT TGCGAAACCA GTTCTTGACG 
GAGAGGAAGA AAGCCGAGAA GCAGGTAGCC AGCCTCAGGG AAGCGCTCAA GACCCAGCGC 
AGCCAGCTGG AGAAGACCCT TCTCGAGCAG AAGCAGGAGA ACAGCTCCAT GCAGAAGGAG 
ATGGCGACTA TCGAGCAGGT GGCGCAGGAC AACCACGAGC GGGCCCGGCG CCTGATGCGG 
GAGCTCAGCC AGATGCAGCA GGAGTACGCG GAGCTCAAGA AGCAGATGGT AAACCAGAAA 
GATCTGGAAA GAAGACAAGT AGAAATCAGT GAAGCGATGA GGACACTTAA GTCTGAAGTG 
AAAGACGAGA TCAGAGCCAG CCTGAAGAAC CTCAACCAGT TTCTCCCGGA GCTGCCAGCA 
GACCTGGAGG CCATTCTGGA AAGGAGCGAA CACCTAGACG GAGGGCTGGA GAGCTTGAAG 
GAGAACTTTC CATTCGCCCT GAGTGAGAAG CCATCACCTT TGGAAGAGAA ACTACATTTT 
TCTCGCGTTC ACATAATGGA TGAGCACTGG CGTGGAGAAG CGCTCCGGGA GAAGCTGCGG 
CACCGTGAGG ACCGACTCAA GGCCCAGCTC CGACACTGTA TGTCCAAGCA GGCAGAGGTG 
TTAAGCAAAG GGAAGCGGCA GACCGAGGGC ACGTTACACA GCCTGAGGAG GCAGGTCGAC 
GCCTTAGGGG AGCTGGTCAC CAGCACGTCT GTGGAGTCCA CGTCGTCAGC CACTCTGTCT 
CAGCTCGAAT CTTCCCTTGC AGAGGACTCC CATCTTGGAC CAAGTCAGAG CTCCTTCCAT 
CAAGTTCCAC AAGGTCCATC ACAATCAGTA GACAGTTACC GACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006054205.1
CDS	129..7136
Translation	MKKGPQQKISKTKMPPSSHSPSPSSLTSNMRSRSLSPLNGSETLPFHSGRQWYGQVEIIGENSMLLDYHDQKEADSHAGVRYITEALVKKLTKQENLTLVKSLNLSLSKDGGKKFKYIENLEKCTKLEILNLSHNLIGKIEKVDKLLKLRELNLSYNKICKIEGIENMHNLQKLNLAGNEIEHIPAWLGKKLKCLRVLNLKANKISSLQDVSKLKPLQDLTSLILAENPVVTLPHYLQFTIFHLRSLESLEGQPVTTQDRQEAFERFSLEEVERLERDLEKKMMETEELKSKQAQFLEEIKNQDKLNRSLKEEAMLQKQSCEELESNLNTKNELLRQKTMELTRACQKQYELEQELAFYKIDAKFEPLNYYPSEYVDVDKAPDESPYIGKSRYKRNMFATESYIVNNAQPVEIKRMEPEEGEQLRNEHVSLKAHMPLDTELEDKENKISTAQARLSALHDEIEKAEQQILRATEEFKQLEEAVQLKKISEAEKDFLLKQLSGRIQLLNKLRQEALDLEMQMEKQRGEIAEKQEEIKDLQRVIDSLDSKDPKHCHMKAQKRGKEQQFDIMNKQYKQLESRLDEILSRIAKETEEIKDLEQQLTEGQIAANEALKKDLEGVISGLQEYLGTVKGQAAQAQSECRRLQDEKEALLQRLAEVEQERDQLEAVALDAEAMRKELAELESALQEQHEVNASLQQTHGDLSAYEAELEAQLRMRDAEASQLKEELGELTRLSQLEQSTLQTELEKEKQALKNALGKAQLSEEKEQENSELRTQLKQLQDDNNLLKQQLKDFQNHLNQVADGLIRPEEVAARVDEVRRKLKSGAGEMSRIHSPSDVLGKSLADLQIQFSEMLTRSQWEKEEAQGRERKLQEEMALHQEKLVDGQEAFRQACERALEARINFDKRQHEARIQQLENEIHYLQENLKSMEEIQGLTDLQLQEADEEKERILAQLQELEKKKKLEDAKSQAQVLGLDKELKKLKKAVATSDKIATAELTIAKDQLKSLHGTVMKINQERAEDLQEAERFSRKAAQAARDLTRAEAEIELLQNLLREREEQMEKVEAGTRGASSQALEIERWNETMERQRSEIARLRDSLDRTGADNRGGIENILEEIAELRREVSYQNDYISSMADPFKRRGYWYFMPPPSSSKVSSHSSQATKDSGVGMKYAASTPIRKPQQNGREDGAPPAASGYWVYSPIRSGLHKSFSNKDADSGGESREESELDDHDDRPFVPPPGYMMYTVFPDGSPVPQGMALYAPPPPLPNNSRPLSPGTVVYGPPPAGAPIVYGPPPPSFSVPLIPVGVLHCNVPEHHALENEVSRLEDIIQHLKSKKQEQRMRVCKRQSEKEIEELHHNIDELLQEKKDLEHEVEELHRTIQKHQQRKDFIDGNVESLMNELKIEKSLKHHEDIVDEIECIEKTLLKRRAELREADRLLAEAESELSRTTEKTKNAVEKFTDAKRNLLQTEKDAEELERRAQETAVSLVKADQQLRLLQADTKDLEQHKTEQEEILKEINKVVAAKDSDFQCLNTKKEKLTEELQKLQKDIETAARTEDHHLQVLRESEALLQAKRAELDKLRSQVTSQQQEMAVLDRQLGHKKEELHLLQGSMVQARADLQEALRLGETEVTEKCNHIRELKGLLEELSFQKGELNVQISEKKNQLSLIKQEIEKEEENLQVVLGQMSKHKTELKNILDMLQLENNELQALKLQHEQKMSELEKAQVEVLEEKLELENLQQTALRQKGEIEWQKQLLERDKREIERITAETRALQLCVESLCKQKEDLEEKCDSWEKKLAQTKRVLAATEENSKAKQSDLEKLELDIRKLEQELDQLNRNKLSLHRDIAAMQQQVQEKREAVNSLQEELADVQAHLNLAKQDLLHTTKRQDVLLGEQTRLQEDLQERRKKLEVCQKEEATKRQHLQALQTEMEANEAELARQETTFQRLQKERESEEKKLEASRATLQEQQQQLEKEIADQKNVLDQVLSQVLGAKERARTLQQEERWGETLEKTLSQTQQQLSEREQQLLEKSAELLALQKEADSMRADFSLLRNQFLTERKKAEKQVASLREALKTQRSQLEKTLLEQKQENSSMQKEMATIEQVAQDNHERARRLMRELSQMQQEYAELKKQMVNQKDLERRQVEISEAMRTLKSEVKDEIRASLKNLNQFLPELPADLEAILERSEHLDGGLESLKENFPFALSEKPSPLEEKLHFSRVHIMDEHWRGEALREKLRHREDRLKAQLRHCMSKQAEVLSKGKRQTEGTLHSLRRQVDALGELVTSTSVESTSSATLSQLESSLAEDSHLGPSQSSFHQVPQGPSQSVDSYRH

Target ORF information:

RefSeq Version	XM_006054143.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centriolin (CNTRL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006054143.1

ORF Insert Sequence:

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCTAAGACA GAAGACCATG 
GAACTAACGC GAGCATGTCA AAAACAGTAT GAACTGGAAC AGGAGTTGGC TTTTTACAAA 
ATCGATGCTA AATTTGAGCC ACTGAATTAT TATCCCTCAG AGTATGTTGA CGTTGATAAA 
GCCCCAGATG AAAGCCCTTA CATTGGCAAG TCCAGATACA AGAGAAATAT GTTCGCCACA 
GAGAGTTACA TTGTGAATAA TGCCCAGCCA GTTGAGATCA AGAGGATGGA GCCAGAGGAA 
GGGGAGCAAC TTAGAAACGA GCATGTGAGC TTGAAAGCCC ATATGCCACT GGACACGGAG 
CTGGAAGACA AAGAGAACAA AATAAGTACA GCACAAGCTC GACTATCAGC ACTGCATGAT 
GAAATAGAAA AGGCAGAACA ACAAATTTTA AGAGCCACTG AAGAATTTAA ACAACTGGAA 
GAAGCTGTAC AACTTAAAAA GATTTCAGAA GCAGAGAAAG ACTTTCTTCT CAAGCAGCTG 
AGTGGCAGGA TACAGCTTCT AAATAAATTA CGCCAGGAAG CTCTTGATCT AGAAATGCAG 
ATGGAAAAGC AAAGAGGAGA AATTGCTGAA AAGCAGGAGG AAATCAAGGA CCTCCAAAGA 
GTTATTGATA GTCTGGATTC CAAAGACCCA AAACATTGCC ATATGAAGGC TCAGAAAAGG 
GGTAAAGAAC AACAATTTGA CATTATGAAC AAGCAGTACA AACAACTTGA GAGCCGTTTG 
GACGAGATAC TTTCTAGAAT TGCTAAAGAA ACTGAAGAGA TTAAGGACCT TGAACAACAG 
CTTACTGAAG GCCAGATAGC AGCAAATGAA GCCCTGAAGA AGGACTTAGA AGGCGTCATC 
AGTGGCCTGC AAGAATACCT GGGCACTGTG AAGGGCCAGG CCGCTCAGGC CCAGAGCGAG 
TGCAGAAGGC TGCAGGATGA GAAAGAGGCG CTGCTGCAGA GGTTGGCCGA GGTCGAGCAG 
GAAAGGGACC AGCTGGAAGC AGTCGCCTTA GATGCAGAAG CTATGAGGAA GGAGCTTGCA 
GAGCTAGAAA GCGCCCTCCA AGAGCAGCAT GAGGTGAACG CGTCCCTGCA GCAGACCCAC 
GGAGACCTCA GTGCCTACGA GGCTGAGCTA GAGGCACAGC TGAGGATGAG GGATGCCGAA 
GCCAGCCAGC TCAAAGAAGA GCTGGGAGAA CTAACAAGGC TCAGCCAGCT AGAACAGTCA 
ACTCTACAAA CAGAACTTGA GAAAGAAAAA CAAGCCCTCA AGAACGCCCT GGGAAAAGCC 
CAGCTCTCGG AAGAAAAAGA ACAAGAAAAC AGTGAGCTCC GCACCCAGCT TAAACAGCTA 
CAGGATGACA ATAACCTGTT AAAACAGCAA CTTAAAGATT TCCAGAATCA CCTTAATCAG 
GTAGCTGATG GTCTGATTCG TCCAGAAGAA GTGGCAGCTC GTGTAGATGA AGTAAGGAGA 
AAATTGAAAT CAGGCGCTGG AGAGATGAGC AGAATCCACA GTCCTTCAGA TGTCTTAGGG 
AAAAGTCTTG CTGACTTGCA GATACAATTC AGTGAAATGC TGACACGTTC TCAGTGGGAG 
AAAGAGGAAG CCCAAGGGAG GGAAAGAAAA CTCCAAGAAG AAATGGCTCT GCATCAGGAG 
AAGCTGGTGG ATGGACAGGA AGCGTTCAGG CAGGCCTGTG AGAGAGCGCT AGAAGCAAGG 
ATTAATTTTG ACAAGAGGCA ACATGAAGCA AGAATTCAGC AGTTAGAGAA TGAGATTCAC 
TATTTACAAG AAAATCTAAA AAGTATGGAG GAAATCCAAG GGCTTACAGA TCTGCAGCTG 
CAGGAAGCTG ATGAAGAGAA GGAGAGAATT CTAGCCCAAC TCCAGGAGTT AGAGAAGAAG 
AAGAAACTTG AAGATGCCAA ATCTCAGGCA CAGGTTCTAG GTTTAGATAA AGAATTGAAG 
AAACTAAAGA AGGCTGTGGC CACCTCTGAT AAGATAGCCA CAGCTGAGCT CACCATCGCC 
AAAGACCAGC TCAAGTCCCT TCATGGAACT GTTATGAAAA TTAACCAGGA GCGTGCAGAG 
GACCTGCAGG AAGCAGAGCG GTTCAGCAGA AAGGCAGCAC AGGCCGCGAG GGATCTGACC 
CGAGCGGAAG CGGAGATCGA ACTCCTGCAG AACCTCCTCA GGGAAAGAGA GGAGCAGATG 
GAGAAGGTAG AGGCAGGCAC CCGAGGAGCA AGCTCACAGG CGCTGGAAAT CGAGAGGTGG 
AATGAGACCA TGGAGAGACA GAGGAGCGAG ATCGCAAGGC TACGGGACTC ACTAGACCGC 
ACCGGGGCGG ATAACAGAGG AGGCATTGAA AATATTTTGG AAGAAATAGC TGAACTCCGA 
CGTGAAGTTT CTTACCAGAA TGATTACATC AGCAGCATGG CAGATCCTTT CAAAAGACGA 
GGCTATTGGT ACTTTATGCC ACCACCATCA TCATCAAAAG TTTCCAGCCA CAGCTCCCAG 
GCCACAAAGG ACTCTGGTGT CGGGATGAAG TACGCAGCCT CGACTCCTAT TAGAAAACCA 
CAGCAGAACG GGAGGGAAGA CGGTGCCCCT CCAGCTGCCT CCGGATACTG GGTGTATTCC 
CCCATCAGGA GCGGCTTACA TAAATCGTTT TCAAATAAAG ATGCAGACAG TGGAGGAGAG 
AGCCGGGAGG AGAGCGAGCT GGATGACCAC GATGACCGCC CGTTCGTCCC TCCTCCTGGA 
TACATGATGT ACACCGTGTT CCCCGACGGC TCTCCTGTAC CACAGGGCAT GGCCCTCTAC 
GCACCGCCGC CTCCCTTGCC CAACAATAGC CGACCTCTCA GCCCTGGCAC TGTTGTGTAT 
GGCCCGCCCC CTGCTGGTGC CCCCATTGTC TACGGGCCCC CACCGCCCAG CTTCTCCGTC 
CCCCTCATCC CAGTGGGCGT GCTGCACTGC AACGTCCCGG AGCACCATGC CTTAGAGAAT 
GAGGTTTCTA GATTGGAAGA CATAATACAG CATTTAAAAT CAAAGAAGCA GGAACAGAGG 
ATGAGAGTAT GCAAGCGGCA GTCTGAGAAA GAAATAGAAG AACTGCATCA TAATATTGAT 
GAACTTTTGC AAGAGAAGAA AGACTTAGAG CACGAAGTAG AAGAATTACA TAGAACCATC 
CAGAAACATC AACAGCGAAA AGACTTCATT GATGGAAATG TTGAGAGTCT TATGAATGAA 
CTAAAAATAG AAAAATCACT CAAGCACCAT GAAGATATTG TAGATGAAAT TGAGTGCATC 
GAGAAGACTC TCCTGAAACG TCGAGCAGAG CTCAGGGAGG CTGACCGACT GCTGGCGGAA 
GCCGAGAGTG AACTTTCACG CACAACAGAG AAAACAAAAA ATGCTGTTGA AAAGTTCACT 
GATGCCAAGA GGAATTTGTT GCAAACTGAG AAAGATGCTG AGGAGTTAGA AAGGAGAGCT 
CAGGAAACAG CTGTTAGCCT TGTCAAGGCT GATCAACAGC TCAGATTGTT ACAGGCGGAC 
ACAAAGGATT TGGAGCAGCA CAAAACTGAG CAAGAAGAGA TCTTGAAAGA GATAAACAAA 
GTTGTTGCAG CAAAAGACTC AGACTTCCAG TGTTTAAACA CGAAGAAGGA GAAGCTAACA 
GAAGAGCTGC AGAAGCTGCA GAAAGACATC GAGACGGCAG CGCGCACGGA GGATCACCAC 
CTGCAGGTCC TGAGAGAGTC GGAGGCCCTC CTGCAGGCCA AGAGAGCGGA GCTGGACAAG 
CTGAGGAGCC AGGTAACGAG TCAGCAGCAG GAGATGGCTG TCTTGGACAG ACAGCTGGGG 
CATAAGAAGG AGGAGCTGCA TCTACTCCAG GGAAGCATGG TCCAGGCCAG AGCTGACCTC 
CAGGAAGCGC TGAGGCTGGG AGAAACTGAA GTAACTGAGA AGTGCAACCA CATCAGGGAA 
CTAAAAGGTC TTCTGGAAGA ACTGAGTTTT CAGAAAGGAG AACTGAATGT CCAGATTAGT 
GAGAAAAAAA ATCAACTTTC ACTTATAAAG CAAGAAATTG AAAAAGAGGA AGAAAATCTT 
CAGGTAGTTT TAGGGCAGAT GTCTAAACAT AAAACTGAAC TTAAGAATAT TCTGGACATG 
TTGCAACTTG AAAACAATGA GCTGCAAGCT TTGAAGCTAC AGCATGAGCA AAAGATGTCT 
GAGTTAGAGA AGGCTCAGGT TGAAGTGCTG GAGGAGAAAC TGGAGTTGGA GAATTTGCAG 
CAGACAGCCC TGCGACAGAA AGGGGAGATC GAGTGGCAGA AGCAGCTCTT AGAGAGGGAC 
AAGCGAGAGA TTGAGCGGAT AACTGCTGAG ACCCGAGCAT TGCAGTTGTG TGTTGAGTCT 
TTGTGCAAAC AAAAGGAAGA TCTTGAGGAG AAATGTGACA GCTGGGAGAA GAAGCTGGCA 
CAGACCAAAC GGGTTTTAGC AGCCACAGAA GAGAATAGCA AAGCAAAGCA ATCAGACTTA 
GAAAAGTTGG AATTGGATAT CAGAAAACTG GAACAGGAAC TAGACCAGCT AAACAGAAAC 
AAACTCTCAC TGCACAGAGA CATCGCGGCC ATGCAGCAGC AGGTCCAAGA AAAACGAGAA 
GCAGTAAACT CATTACAGGA GGAACTAGCT GATGTCCAAG CCCATTTGAA CCTAGCGAAA 
CAGGACCTGC TCCACACCAC CAAGCGCCAG GATGTCCTGC TTGGCGAGCA GACCAGGCTG 
CAGGAGGACC TCCAGGAACG CAGAAAGAAG TTGGAAGTCT GCCAAAAAGA AGAGGCAACA 
AAGCGGCAGC ACCTGCAAGC GCTTCAGACG GAGATGGAGG CGAACGAGGC CGAGCTGGCC 
CGGCAGGAGA CGACATTTCA GAGACTCCAG AAAGAGAGAG AAAGTGAAGA AAAAAAGTTA 
GAAGCCAGTC GAGCGACTCT GCAGGAGCAG CAGCAGCAGC TGGAAAAGGA AATCGCAGAC 
CAGAAGAACG TGCTGGACCA GGTGCTCAGC CAGGTGCTGG GGGCCAAGGA GCGCGCCAGG 
ACTCTGCAGC AGGAGGAGAG GTGGGGTGAG ACCCTGGAGA AGACGCTCTC CCAGACCCAG 
CAACAGCTCT CAGAACGGGA GCAGCAGTTG TTGGAAAAGT CAGCTGAGCT GCTGGCTCTT 
CAGAAAGAGG CAGACTCGAT GAGGGCAGAC TTCAGCCTCT TGCGAAACCA GTTCTTGACG 
GAGAGGAAGA AAGCCGAGAA GCAGGTAGCC AGCCTCAGGG AAGCGCTCAA GACCCAGCGC 
AGCCAGCTGG AGAAGACCCT TCTCGAGCAG AAGCAGGAGA ACAGCTCCAT GCAGAAGGAG 
ATGGCGACTA TCGAGCAGGT GGCGCAGGAC AACCACGAGC GGGCCCGGCG CCTGATGCGG 
GAGCTCAGCC AGATGCAGCA GGAGTACGCG GAGCTCAAGA AGCAGATGGT AAACCAGAAA 
GATCTGGAAA GAAGACAAGT AGAAATCAGT GAAGCGATGA GGACACTTAA GTCTGAAGTG 
AAAGACGAGA TCAGAGCCAG CCTGAAGAAC CTCAACCAGT TTCTCCCGGA GCTGCCAGCA 
GACCTGGAGG CCATTCTGGA AAGGAGCGAA CACCTAGACG GAGGGCTGGA GAGCTTGAAG 
GAGAACTTTC CATTCGCCCT GAGTGAGAAG CCATCACCTT TGGAAGAGAA ACTACATTTT 
TCTCGCGTTC ACATAATGGA TGAGCACTGG CGTGGAGAAG CGCTCCGGGA GAAGCTGCGG 
CACCGTGAGG ACCGACTCAA GGCCCAGCTC CGACACTGTA TGTCCAAGCA GGCAGAGGTG 
TTAAGCAAAG GGAAGCGGCA GACCGAGGGC ACGTTACACA GCCTGAGGAG GCAGGTCGAC 
GCCTTAGGGG AGCTGGTCAC CAGCACGTCT GTGGAGTCCA CGTCGTCAGC CACTCTGTCT 
CAGCTCGAAT CTTCCCTTGC AGAGGACTCC CATCTTGGAC CAAGTCAGAG CTCCTTCCAT 
CAAGTTCCAC AAGGTCCATC ACAATCAGTA GACAGTTACC GACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTRL ( XM_006054144.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006054206.1 Bubalus bubalis centriolin (CNTRL), transcript variant X2, mRNA.

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CloneID	OBu11629
Clone ID Related Accession (Same CDS sequence)	XM_006054144.1
Accession Version	XM_006054144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	centriolin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784589.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCTAAGACA GAAGACCATG 
GAACTAACGC GAGCATGTCA AAAACAGTAT GAACTGGAAC AGGAGTTGGC TTTTTACAAA 
ATCGATGCTA AATTTGAGCC ACTGAATTAT TATCCCTCAG AGTATGTTGA CGTTGATAAA 
GCCCCAGATG AAAGCCCTTA CATTGGCAAG TCCAGATACA AGAGAAATAT GTTCGCCACA 
GAGAGTTACA TTGTGAATAA TGCCCAGCCA GTTGAGATCA AGAGGATGGA GCCAGAGGAA 
GGGGAGCAAC TTAGAAACGA GCATGTGAGC TTGAAAGCCC ATATGCCACT GGACACGGAG 
CTGGAAGACA AAGAGAACAA AATAAGTACA GCACAAGCTC GACTATCAGC ACTGCATGAT 
GAAATAGAAA AGGCAGAACA ACAAATTTTA AGAGCCACTG AAGAATTTAA ACAACTGGAA 
GAAGCTGTAC AACTTAAAAA GATTTCAGAA GCAGAGAAAG ACTTTCTTCT CAAGCAGCTG 
AGTGGCAGGA TACAGCTTCT AAATAAATTA CGCCAGGAAG CTCTTGATCT AGAAATGCAG 
ATGGAAAAGC AAAGAGGAGA AATTGCTGAA AAGCAGGAGG AAATCAAGGA CCTCCAAAGA 
GTTATTGATA GTCTGGATTC CAAAGACCCA AAACATTGCC ATATGAAGGC TCAGAAAAGG 
GGTAAAGAAC AACAATTTGA CATTATGAAC AAGCAGTACA AACAACTTGA GAGCCGTTTG 
GACGAGATAC TTTCTAGAAT TGCTAAAGAA ACTGAAGAGA TTAAGGACCT TGAACAACAG 
CTTACTGAAG GCCAGATAGC AGCAAATGAA GCCCTGAAGA AGGACTTAGA AGGCGTCATC 
AGTGGCCTGC AAGAATACCT GGGCACTGTG AAGGGCCAGG CCGCTCAGGC CCAGAGCGAG 
TGCAGAAGGC TGCAGGATGA GAAAGAGGCG CTGCTGCAGA GGTTGGCCGA GGTCGAGCAG 
GAAAGGGACC AGCTGGAAGC AGTCGCCTTA GATGCAGAAG CTATGAGGAA GGAGCTTGCA 
GAGCTAGAAA GCGCCCTCCA AGAGCAGCAT GAGGTGAACG CGTCCCTGCA GCAGACCCAC 
GGAGACCTCA GTGCCTACGA GGCTGAGCTA GAGGCACAGC TGAGGATGAG GGATGCCGAA 
GCCAGCCAGC TCAAAGAAGA GCTGGGAGAA CTAACAAGGC TCAGCCAGCT AGAACAGTCA 
ACTCTACAAA CAGAACTTGA GAAAGAAAAA CAAGCCCTCA AGAACGCCCT GGGAAAAGCC 
CAGCTCTCGG AAGAAAAAGA ACAAGAAAAC AGTGAGCTCC GCACCCAGCT TAAACAGCTA 
CAGGATGACA ATAACCTGTT AAAACAGCAA CTTAAAGATT TCCAGAATCA CCTTAATCAG 
GTAGCTGATG GTCTGATTCG TCCAGAAGAA GTGGCAGCTC GTGTAGATGA AGTAAGGAGA 
AAATTGAAAT CAGGCGCTGG AGAGATGAGA ATCCACAGTC CTTCAGATGT CTTAGGGAAA 
AGTCTTGCTG ACTTGCAGAT ACAATTCAGT GAAATGCTGA CACGTTCTCA GTGGGAGAAA 
GAGGAAGCCC AAGGGAGGGA AAGAAAACTC CAAGAAGAAA TGGCTCTGCA TCAGGAGAAG 
CTGGTGGATG GACAGGAAGC GTTCAGGCAG GCCTGTGAGA GAGCGCTAGA AGCAAGGATT 
AATTTTGACA AGAGGCAACA TGAAGCAAGA ATTCAGCAGT TAGAGAATGA GATTCACTAT 
TTACAAGAAA ATCTAAAAAG TATGGAGGAA ATCCAAGGGC TTACAGATCT GCAGCTGCAG 
GAAGCTGATG AAGAGAAGGA GAGAATTCTA GCCCAACTCC AGGAGTTAGA GAAGAAGAAG 
AAACTTGAAG ATGCCAAATC TCAGGCACAG GTTCTAGGTT TAGATAAAGA ATTGAAGAAA 
CTAAAGAAGG CTGTGGCCAC CTCTGATAAG ATAGCCACAG CTGAGCTCAC CATCGCCAAA 
GACCAGCTCA AGTCCCTTCA TGGAACTGTT ATGAAAATTA ACCAGGAGCG TGCAGAGGAC 
CTGCAGGAAG CAGAGCGGTT CAGCAGAAAG GCAGCACAGG CCGCGAGGGA TCTGACCCGA 
GCGGAAGCGG AGATCGAACT CCTGCAGAAC CTCCTCAGGG AAAGAGAGGA GCAGCTTCGA 
ATTGAGATGG AGAAGGTAGA GGCAGGCACC CGAGGAGCAA GCTCACAGGC GCTGGAAATC 
GAGAGGTGGA ATGAGACCAT GGAGAGACAG AGGAGCGAGA TCGCAAGGCT ACGGGACTCA 
CTAGACCGCA CCGGGGCGGA TAACAGAGGA GGCATTGAAA ATATTTTGGA AGAAATAGCT 
GAACTCCGAC GTGAAGTTTC TTACCAGAAT GATTACATCA GCAGCATGGC AGATCCTTTC 
AAAAGACGAG GCTATTGGTA CTTTATGCCA CCACCATCAT CATCAAAAGT TTCCAGCCAC 
AGCTCCCAGG CCACAAAGGA CTCTGGTGTC GGGATGAAGT ACGCAGCCTC GACTCCTATT 
AGAAAACCAC AGCAGAACGG GAGGGAAGAC GGTGCCCCTC CAGCTGCCTC CGGATACTGG 
GTGTATTCCC CCATCAGGAG CGGCTTACAT AAATCGTTTT CAAATAAAGA TGCAGACAGT 
GGAGGAGAGA GCCGGGAGGA GAGCGAGCTG GATGACCACG ATGACCGCCC GTTCGTCCCT 
CCTCCTGGAT ACATGATGTA CACCGTGTTC CCCGACGGCT CTCCTGTACC ACAGGGCATG 
GCCCTCTACG CACCGCCGCC TCCCTTGCCC AACAATAGCC GACCTCTCAG CCCTGGCACT 
GTTGTGTATG GCCCGCCCCC TGCTGGTGCC CCCATTGTCT ACGGGCCCCC ACCGCCCAGC 
TTCTCCGTCC CCCTCATCCC AGTGGGCGTG CTGCACTGCA ACGTCCCGGA GCACCATGCC 
TTAGAGAATG AGGTTTCTAG ATTGGAAGAC ATAATACAGC ATTTAAAATC AAAGAAGCAG 
GAACAGAGGA TGAGAGTATG CAAGCGGCAG TCTGAGAAAG AAATAGAAGA ACTGCATCAT 
AATATTGATG AACTTTTGCA AGAGAAGAAA GACTTAGAGC ACGAAGTAGA AGAATTACAT 
AGAACCATCC AGAAACATCA ACAGCGAAAA GACTTCATTG ATGGAAATGT TGAGAGTCTT 
ATGAATGAAC TAAAAATAGA AAAATCACTC AAGCACCATG AAGATATTGT AGATGAAATT 
GAGTGCATCG AGAAGACTCT CCTGAAACGT CGAGCAGAGC TCAGGGAGGC TGACCGACTG 
CTGGCGGAAG CCGAGAGTGA ACTTTCACGC ACAACAGAGA AAACAAAAAA TGCTGTTGAA 
AAGTTCACTG ATGCCAAGAG GAATTTGTTG CAAACTGAGA AAGATGCTGA GGAGTTAGAA 
AGGAGAGCTC AGGAAACAGC TGTTAGCCTT GTCAAGGCTG ATCAACAGCT CAGATTGTTA 
CAGGCGGACA CAAAGGATTT GGAGCAGCAC AAAACTGAGC AAGAAGAGAT CTTGAAAGAG 
ATAAACAAAG TTGTTGCAGC AAAAGACTCA GACTTCCAGT GTTTAAACAC GAAGAAGGAG 
AAGCTAACAG AAGAGCTGCA GAAGCTGCAG AAAGACATCG AGACGGCAGC GCGCACGGAG 
GATCACCACC TGCAGGTCCT GAGAGAGTCG GAGGCCCTCC TGCAGGCCAA GAGAGCGGAG 
CTGGACAAGC TGAGGAGCCA GGTAACGAGT CAGCAGCAGG AGATGGCTGT CTTGGACAGA 
CAGCTGGGGC ATAAGAAGGA GGAGCTGCAT CTACTCCAGG GAAGCATGGT CCAGGCCAGA 
GCTGACCTCC AGGAAGCGCT GAGGCTGGGA GAAACTGAAG TAACTGAGAA GTGCAACCAC 
ATCAGGGAAC TAAAAGGTCT TCTGGAAGAA CTGAGTTTTC AGAAAGGAGA ACTGAATGTC 
CAGATTAGTG AGAAAAAAAA TCAACTTTCA CTTATAAAGC AAGAAATTGA AAAAGAGGAA 
GAAAATCTTC AGGTAGTTTT AGGGCAGATG TCTAAACATA AAACTGAACT TAAGAATATT 
CTGGACATGT TGCAACTTGA AAACAATGAG CTGCAAGCTT TGAAGCTACA GCATGAGCAA 
AAGATGTCTG AGTTAGAGAA GGCTCAGGTT GAAGTGCTGG AGGAGAAACT GGAGTTGGAG 
AATTTGCAGC AGACAGCCCT GCGACAGAAA GGGGAGATCG AGTGGCAGAA GCAGCTCTTA 
GAGAGGGACA AGCGAGAGAT TGAGCGGATA ACTGCTGAGA CCCGAGCATT GCAGTTGTGT 
GTTGAGTCTT TGTGCAAACA AAAGGAAGAT CTTGAGGAGA AATGTGACAG CTGGGAGAAG 
AAGCTGGCAC AGACCAAACG GGTTTTAGCA GCCACAGAAG AGAATAGCAA AGCAAAGCAA 
TCAGACTTAG AAAAGTTGGA ATTGGATATC AGAAAACTGG AACAGGAACT AGACCAGCTA 
AACAGAAACA AACTCTCACT GCACAGAGAC ATCGCGGCCA TGCAGCAGCA GGTCCAAGAA 
AAACGAGAAG CAGTAAACTC ATTACAGGAG GAACTAGCTG ATGTCCAAGC CCATTTGAAC 
CTAGCGAAAC AGGACCTGCT CCACACCACC AAGCGCCAGG ATGTCCTGCT TGGCGAGCAG 
ACCAGGCTGC AGGAGGACCT CCAGGAACGC AGAAAGAAGT TGGAAGTCTG CCAAAAAGAA 
GAGGCAACAA AGCGGCAGCA CCTGCAAGCG CTTCAGACGG AGATGGAGGC GAACGAGGCC 
GAGCTGGCCC GGCAGGAGAC GACATTTCAG AGACTCCAGA AAGAGAGAGA AAGTGAAGAA 
AAAAAGTTAG AAGCCAGTCG AGCGACTCTG CAGGAGCAGC AGCAGCAGCT GGAAAAGGAA 
ATCGCAGACC AGAAGAACGT GCTGGACCAG GTGCTCAGCC AGGTGCTGGG GGCCAAGGAG 
CGCGCCAGGA CTCTGCAGCA GGAGGAGAGG TGGGGTGAGA CCCTGGAGAA GACGCTCTCC 
CAGACCCAGC AACAGCTCTC AGAACGGGAG CAGCAGTTGT TGGAAAAGTC AGCTGAGCTG 
CTGGCTCTTC AGAAAGAGGC AGACTCGATG AGGGCAGACT TCAGCCTCTT GCGAAACCAG 
TTCTTGACGG AGAGGAAGAA AGCCGAGAAG CAGGTAGCCA GCCTCAGGGA AGCGCTCAAG 
ACCCAGCGCA GCCAGCTGGA GAAGACCCTT CTCGAGCAGA AGCAGGAGAA CAGCTCCATG 
CAGAAGGAGA TGGCGACTAT CGAGCAGGTG GCGCAGGACA ACCACGAGCG GGCCCGGCGC 
CTGATGCGGG AGCTCAGCCA GATGCAGCAG GAGTACGCGG AGCTCAAGAA GCAGATGGTA 
AACCAGAAAG ATCTGGAAAG AAGACAAGTA GAAATCAGTG AAGCGATGAG GACACTTAAG 
TCTGAAGTGA AAGACGAGAT CAGAGCCAGC CTGAAGAACC TCAACCAGTT TCTCCCGGAG 
CTGCCAGCAG ACCTGGAGGC CATTCTGGAA AGGAGCGAAC ACCTAGACGG AGGGCTGGAG 
AGCTTGAAGG AGAACTTTCC ATTCGCCCTG AGTGAGAAGC CATCACCTTT GGAAGAGAAA 
CTACATTTTT CTCGCGTTCA CATAATGGAT GAGCACTGGC GTGGAGAAGC GCTCCGGGAG 
AAGCTGCGGC ACCGTGAGGA CCGACTCAAG GCCCAGCTCC GACACTGTAT GTCCAAGCAG 
GCAGAGGTGT TAAGCAAAGG GAAGCGGCAG ACCGAGGGCA CGTTACACAG CCTGAGGAGG 
CAGGTCGACG CCTTAGGGGA GCTGGTCACC AGCACGTCTG TGGAGTCCAC GTCGTCAGCC 
ACTCTGTCTC AGCTCGAATC TTCCCTTGCA GAGGACTCCC ATCTTGGACC AAGTCAGAGC 
TCCTTCCATC AAGTTCCACA AGGTCCATCA CAATCAGTAG ACAGTTACCG ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006054206.1
CDS	129..7145
Translation	MKKGPQQKISKTKMPPSSHSPSPSSLTSNMRSRSLSPLNGSETLPFHSGRQWYGQVEIIGENSMLLDYHDQKEADSHAGVRYITEALVKKLTKQENLTLVKSLNLSLSKDGGKKFKYIENLEKCTKLEILNLSHNLIGKIEKVDKLLKLRELNLSYNKICKIEGIENMHNLQKLNLAGNEIEHIPAWLGKKLKCLRVLNLKANKISSLQDVSKLKPLQDLTSLILAENPVVTLPHYLQFTIFHLRSLESLEGQPVTTQDRQEAFERFSLEEVERLERDLEKKMMETEELKSKQAQFLEEIKNQDKLNRSLKEEAMLQKQSCEELESNLNTKNELLRQKTMELTRACQKQYELEQELAFYKIDAKFEPLNYYPSEYVDVDKAPDESPYIGKSRYKRNMFATESYIVNNAQPVEIKRMEPEEGEQLRNEHVSLKAHMPLDTELEDKENKISTAQARLSALHDEIEKAEQQILRATEEFKQLEEAVQLKKISEAEKDFLLKQLSGRIQLLNKLRQEALDLEMQMEKQRGEIAEKQEEIKDLQRVIDSLDSKDPKHCHMKAQKRGKEQQFDIMNKQYKQLESRLDEILSRIAKETEEIKDLEQQLTEGQIAANEALKKDLEGVISGLQEYLGTVKGQAAQAQSECRRLQDEKEALLQRLAEVEQERDQLEAVALDAEAMRKELAELESALQEQHEVNASLQQTHGDLSAYEAELEAQLRMRDAEASQLKEELGELTRLSQLEQSTLQTELEKEKQALKNALGKAQLSEEKEQENSELRTQLKQLQDDNNLLKQQLKDFQNHLNQVADGLIRPEEVAARVDEVRRKLKSGAGEMRIHSPSDVLGKSLADLQIQFSEMLTRSQWEKEEAQGRERKLQEEMALHQEKLVDGQEAFRQACERALEARINFDKRQHEARIQQLENEIHYLQENLKSMEEIQGLTDLQLQEADEEKERILAQLQELEKKKKLEDAKSQAQVLGLDKELKKLKKAVATSDKIATAELTIAKDQLKSLHGTVMKINQERAEDLQEAERFSRKAAQAARDLTRAEAEIELLQNLLREREEQLRIEMEKVEAGTRGASSQALEIERWNETMERQRSEIARLRDSLDRTGADNRGGIENILEEIAELRREVSYQNDYISSMADPFKRRGYWYFMPPPSSSKVSSHSSQATKDSGVGMKYAASTPIRKPQQNGREDGAPPAASGYWVYSPIRSGLHKSFSNKDADSGGESREESELDDHDDRPFVPPPGYMMYTVFPDGSPVPQGMALYAPPPPLPNNSRPLSPGTVVYGPPPAGAPIVYGPPPPSFSVPLIPVGVLHCNVPEHHALENEVSRLEDIIQHLKSKKQEQRMRVCKRQSEKEIEELHHNIDELLQEKKDLEHEVEELHRTIQKHQQRKDFIDGNVESLMNELKIEKSLKHHEDIVDEIECIEKTLLKRRAELREADRLLAEAESELSRTTEKTKNAVEKFTDAKRNLLQTEKDAEELERRAQETAVSLVKADQQLRLLQADTKDLEQHKTEQEEILKEINKVVAAKDSDFQCLNTKKEKLTEELQKLQKDIETAARTEDHHLQVLRESEALLQAKRAELDKLRSQVTSQQQEMAVLDRQLGHKKEELHLLQGSMVQARADLQEALRLGETEVTEKCNHIRELKGLLEELSFQKGELNVQISEKKNQLSLIKQEIEKEEENLQVVLGQMSKHKTELKNILDMLQLENNELQALKLQHEQKMSELEKAQVEVLEEKLELENLQQTALRQKGEIEWQKQLLERDKREIERITAETRALQLCVESLCKQKEDLEEKCDSWEKKLAQTKRVLAATEENSKAKQSDLEKLELDIRKLEQELDQLNRNKLSLHRDIAAMQQQVQEKREAVNSLQEELADVQAHLNLAKQDLLHTTKRQDVLLGEQTRLQEDLQERRKKLEVCQKEEATKRQHLQALQTEMEANEAELARQETTFQRLQKERESEEKKLEASRATLQEQQQQLEKEIADQKNVLDQVLSQVLGAKERARTLQQEERWGETLEKTLSQTQQQLSEREQQLLEKSAELLALQKEADSMRADFSLLRNQFLTERKKAEKQVASLREALKTQRSQLEKTLLEQKQENSSMQKEMATIEQVAQDNHERARRLMRELSQMQQEYAELKKQMVNQKDLERRQVEISEAMRTLKSEVKDEIRASLKNLNQFLPELPADLEAILERSEHLDGGLESLKENFPFALSEKPSPLEEKLHFSRVHIMDEHWRGEALREKLRHREDRLKAQLRHCMSKQAEVLSKGKRQTEGTLHSLRRQVDALGELVTSTSVESTSSATLSQLESSLAEDSHLGPSQSSFHQVPQGPSQSVDSYRH

Target ORF information:

RefSeq Version	XM_006054144.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centriolin (CNTRL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006054144.1

ORF Insert Sequence:

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCTAAGACA GAAGACCATG 
GAACTAACGC GAGCATGTCA AAAACAGTAT GAACTGGAAC AGGAGTTGGC TTTTTACAAA 
ATCGATGCTA AATTTGAGCC ACTGAATTAT TATCCCTCAG AGTATGTTGA CGTTGATAAA 
GCCCCAGATG AAAGCCCTTA CATTGGCAAG TCCAGATACA AGAGAAATAT GTTCGCCACA 
GAGAGTTACA TTGTGAATAA TGCCCAGCCA GTTGAGATCA AGAGGATGGA GCCAGAGGAA 
GGGGAGCAAC TTAGAAACGA GCATGTGAGC TTGAAAGCCC ATATGCCACT GGACACGGAG 
CTGGAAGACA AAGAGAACAA AATAAGTACA GCACAAGCTC GACTATCAGC ACTGCATGAT 
GAAATAGAAA AGGCAGAACA ACAAATTTTA AGAGCCACTG AAGAATTTAA ACAACTGGAA 
GAAGCTGTAC AACTTAAAAA GATTTCAGAA GCAGAGAAAG ACTTTCTTCT CAAGCAGCTG 
AGTGGCAGGA TACAGCTTCT AAATAAATTA CGCCAGGAAG CTCTTGATCT AGAAATGCAG 
ATGGAAAAGC AAAGAGGAGA AATTGCTGAA AAGCAGGAGG AAATCAAGGA CCTCCAAAGA 
GTTATTGATA GTCTGGATTC CAAAGACCCA AAACATTGCC ATATGAAGGC TCAGAAAAGG 
GGTAAAGAAC AACAATTTGA CATTATGAAC AAGCAGTACA AACAACTTGA GAGCCGTTTG 
GACGAGATAC TTTCTAGAAT TGCTAAAGAA ACTGAAGAGA TTAAGGACCT TGAACAACAG 
CTTACTGAAG GCCAGATAGC AGCAAATGAA GCCCTGAAGA AGGACTTAGA AGGCGTCATC 
AGTGGCCTGC AAGAATACCT GGGCACTGTG AAGGGCCAGG CCGCTCAGGC CCAGAGCGAG 
TGCAGAAGGC TGCAGGATGA GAAAGAGGCG CTGCTGCAGA GGTTGGCCGA GGTCGAGCAG 
GAAAGGGACC AGCTGGAAGC AGTCGCCTTA GATGCAGAAG CTATGAGGAA GGAGCTTGCA 
GAGCTAGAAA GCGCCCTCCA AGAGCAGCAT GAGGTGAACG CGTCCCTGCA GCAGACCCAC 
GGAGACCTCA GTGCCTACGA GGCTGAGCTA GAGGCACAGC TGAGGATGAG GGATGCCGAA 
GCCAGCCAGC TCAAAGAAGA GCTGGGAGAA CTAACAAGGC TCAGCCAGCT AGAACAGTCA 
ACTCTACAAA CAGAACTTGA GAAAGAAAAA CAAGCCCTCA AGAACGCCCT GGGAAAAGCC 
CAGCTCTCGG AAGAAAAAGA ACAAGAAAAC AGTGAGCTCC GCACCCAGCT TAAACAGCTA 
CAGGATGACA ATAACCTGTT AAAACAGCAA CTTAAAGATT TCCAGAATCA CCTTAATCAG 
GTAGCTGATG GTCTGATTCG TCCAGAAGAA GTGGCAGCTC GTGTAGATGA AGTAAGGAGA 
AAATTGAAAT CAGGCGCTGG AGAGATGAGA ATCCACAGTC CTTCAGATGT CTTAGGGAAA 
AGTCTTGCTG ACTTGCAGAT ACAATTCAGT GAAATGCTGA CACGTTCTCA GTGGGAGAAA 
GAGGAAGCCC AAGGGAGGGA AAGAAAACTC CAAGAAGAAA TGGCTCTGCA TCAGGAGAAG 
CTGGTGGATG GACAGGAAGC GTTCAGGCAG GCCTGTGAGA GAGCGCTAGA AGCAAGGATT 
AATTTTGACA AGAGGCAACA TGAAGCAAGA ATTCAGCAGT TAGAGAATGA GATTCACTAT 
TTACAAGAAA ATCTAAAAAG TATGGAGGAA ATCCAAGGGC TTACAGATCT GCAGCTGCAG 
GAAGCTGATG AAGAGAAGGA GAGAATTCTA GCCCAACTCC AGGAGTTAGA GAAGAAGAAG 
AAACTTGAAG ATGCCAAATC TCAGGCACAG GTTCTAGGTT TAGATAAAGA ATTGAAGAAA 
CTAAAGAAGG CTGTGGCCAC CTCTGATAAG ATAGCCACAG CTGAGCTCAC CATCGCCAAA 
GACCAGCTCA AGTCCCTTCA TGGAACTGTT ATGAAAATTA ACCAGGAGCG TGCAGAGGAC 
CTGCAGGAAG CAGAGCGGTT CAGCAGAAAG GCAGCACAGG CCGCGAGGGA TCTGACCCGA 
GCGGAAGCGG AGATCGAACT CCTGCAGAAC CTCCTCAGGG AAAGAGAGGA GCAGCTTCGA 
ATTGAGATGG AGAAGGTAGA GGCAGGCACC CGAGGAGCAA GCTCACAGGC GCTGGAAATC 
GAGAGGTGGA ATGAGACCAT GGAGAGACAG AGGAGCGAGA TCGCAAGGCT ACGGGACTCA 
CTAGACCGCA CCGGGGCGGA TAACAGAGGA GGCATTGAAA ATATTTTGGA AGAAATAGCT 
GAACTCCGAC GTGAAGTTTC TTACCAGAAT GATTACATCA GCAGCATGGC AGATCCTTTC 
AAAAGACGAG GCTATTGGTA CTTTATGCCA CCACCATCAT CATCAAAAGT TTCCAGCCAC 
AGCTCCCAGG CCACAAAGGA CTCTGGTGTC GGGATGAAGT ACGCAGCCTC GACTCCTATT 
AGAAAACCAC AGCAGAACGG GAGGGAAGAC GGTGCCCCTC CAGCTGCCTC CGGATACTGG 
GTGTATTCCC CCATCAGGAG CGGCTTACAT AAATCGTTTT CAAATAAAGA TGCAGACAGT 
GGAGGAGAGA GCCGGGAGGA GAGCGAGCTG GATGACCACG ATGACCGCCC GTTCGTCCCT 
CCTCCTGGAT ACATGATGTA CACCGTGTTC CCCGACGGCT CTCCTGTACC ACAGGGCATG 
GCCCTCTACG CACCGCCGCC TCCCTTGCCC AACAATAGCC GACCTCTCAG CCCTGGCACT 
GTTGTGTATG GCCCGCCCCC TGCTGGTGCC CCCATTGTCT ACGGGCCCCC ACCGCCCAGC 
TTCTCCGTCC CCCTCATCCC AGTGGGCGTG CTGCACTGCA ACGTCCCGGA GCACCATGCC 
TTAGAGAATG AGGTTTCTAG ATTGGAAGAC ATAATACAGC ATTTAAAATC AAAGAAGCAG 
GAACAGAGGA TGAGAGTATG CAAGCGGCAG TCTGAGAAAG AAATAGAAGA ACTGCATCAT 
AATATTGATG AACTTTTGCA AGAGAAGAAA GACTTAGAGC ACGAAGTAGA AGAATTACAT 
AGAACCATCC AGAAACATCA ACAGCGAAAA GACTTCATTG ATGGAAATGT TGAGAGTCTT 
ATGAATGAAC TAAAAATAGA AAAATCACTC AAGCACCATG AAGATATTGT AGATGAAATT 
GAGTGCATCG AGAAGACTCT CCTGAAACGT CGAGCAGAGC TCAGGGAGGC TGACCGACTG 
CTGGCGGAAG CCGAGAGTGA ACTTTCACGC ACAACAGAGA AAACAAAAAA TGCTGTTGAA 
AAGTTCACTG ATGCCAAGAG GAATTTGTTG CAAACTGAGA AAGATGCTGA GGAGTTAGAA 
AGGAGAGCTC AGGAAACAGC TGTTAGCCTT GTCAAGGCTG ATCAACAGCT CAGATTGTTA 
CAGGCGGACA CAAAGGATTT GGAGCAGCAC AAAACTGAGC AAGAAGAGAT CTTGAAAGAG 
ATAAACAAAG TTGTTGCAGC AAAAGACTCA GACTTCCAGT GTTTAAACAC GAAGAAGGAG 
AAGCTAACAG AAGAGCTGCA GAAGCTGCAG AAAGACATCG AGACGGCAGC GCGCACGGAG 
GATCACCACC TGCAGGTCCT GAGAGAGTCG GAGGCCCTCC TGCAGGCCAA GAGAGCGGAG 
CTGGACAAGC TGAGGAGCCA GGTAACGAGT CAGCAGCAGG AGATGGCTGT CTTGGACAGA 
CAGCTGGGGC ATAAGAAGGA GGAGCTGCAT CTACTCCAGG GAAGCATGGT CCAGGCCAGA 
GCTGACCTCC AGGAAGCGCT GAGGCTGGGA GAAACTGAAG TAACTGAGAA GTGCAACCAC 
ATCAGGGAAC TAAAAGGTCT TCTGGAAGAA CTGAGTTTTC AGAAAGGAGA ACTGAATGTC 
CAGATTAGTG AGAAAAAAAA TCAACTTTCA CTTATAAAGC AAGAAATTGA AAAAGAGGAA 
GAAAATCTTC AGGTAGTTTT AGGGCAGATG TCTAAACATA AAACTGAACT TAAGAATATT 
CTGGACATGT TGCAACTTGA AAACAATGAG CTGCAAGCTT TGAAGCTACA GCATGAGCAA 
AAGATGTCTG AGTTAGAGAA GGCTCAGGTT GAAGTGCTGG AGGAGAAACT GGAGTTGGAG 
AATTTGCAGC AGACAGCCCT GCGACAGAAA GGGGAGATCG AGTGGCAGAA GCAGCTCTTA 
GAGAGGGACA AGCGAGAGAT TGAGCGGATA ACTGCTGAGA CCCGAGCATT GCAGTTGTGT 
GTTGAGTCTT TGTGCAAACA AAAGGAAGAT CTTGAGGAGA AATGTGACAG CTGGGAGAAG 
AAGCTGGCAC AGACCAAACG GGTTTTAGCA GCCACAGAAG AGAATAGCAA AGCAAAGCAA 
TCAGACTTAG AAAAGTTGGA ATTGGATATC AGAAAACTGG AACAGGAACT AGACCAGCTA 
AACAGAAACA AACTCTCACT GCACAGAGAC ATCGCGGCCA TGCAGCAGCA GGTCCAAGAA 
AAACGAGAAG CAGTAAACTC ATTACAGGAG GAACTAGCTG ATGTCCAAGC CCATTTGAAC 
CTAGCGAAAC AGGACCTGCT CCACACCACC AAGCGCCAGG ATGTCCTGCT TGGCGAGCAG 
ACCAGGCTGC AGGAGGACCT CCAGGAACGC AGAAAGAAGT TGGAAGTCTG CCAAAAAGAA 
GAGGCAACAA AGCGGCAGCA CCTGCAAGCG CTTCAGACGG AGATGGAGGC GAACGAGGCC 
GAGCTGGCCC GGCAGGAGAC GACATTTCAG AGACTCCAGA AAGAGAGAGA AAGTGAAGAA 
AAAAAGTTAG AAGCCAGTCG AGCGACTCTG CAGGAGCAGC AGCAGCAGCT GGAAAAGGAA 
ATCGCAGACC AGAAGAACGT GCTGGACCAG GTGCTCAGCC AGGTGCTGGG GGCCAAGGAG 
CGCGCCAGGA CTCTGCAGCA GGAGGAGAGG TGGGGTGAGA CCCTGGAGAA GACGCTCTCC 
CAGACCCAGC AACAGCTCTC AGAACGGGAG CAGCAGTTGT TGGAAAAGTC AGCTGAGCTG 
CTGGCTCTTC AGAAAGAGGC AGACTCGATG AGGGCAGACT TCAGCCTCTT GCGAAACCAG 
TTCTTGACGG AGAGGAAGAA AGCCGAGAAG CAGGTAGCCA GCCTCAGGGA AGCGCTCAAG 
ACCCAGCGCA GCCAGCTGGA GAAGACCCTT CTCGAGCAGA AGCAGGAGAA CAGCTCCATG 
CAGAAGGAGA TGGCGACTAT CGAGCAGGTG GCGCAGGACA ACCACGAGCG GGCCCGGCGC 
CTGATGCGGG AGCTCAGCCA GATGCAGCAG GAGTACGCGG AGCTCAAGAA GCAGATGGTA 
AACCAGAAAG ATCTGGAAAG AAGACAAGTA GAAATCAGTG AAGCGATGAG GACACTTAAG 
TCTGAAGTGA AAGACGAGAT CAGAGCCAGC CTGAAGAACC TCAACCAGTT TCTCCCGGAG 
CTGCCAGCAG ACCTGGAGGC CATTCTGGAA AGGAGCGAAC ACCTAGACGG AGGGCTGGAG 
AGCTTGAAGG AGAACTTTCC ATTCGCCCTG AGTGAGAAGC CATCACCTTT GGAAGAGAAA 
CTACATTTTT CTCGCGTTCA CATAATGGAT GAGCACTGGC GTGGAGAAGC GCTCCGGGAG 
AAGCTGCGGC ACCGTGAGGA CCGACTCAAG GCCCAGCTCC GACACTGTAT GTCCAAGCAG 
GCAGAGGTGT TAAGCAAAGG GAAGCGGCAG ACCGAGGGCA CGTTACACAG CCTGAGGAGG 
CAGGTCGACG CCTTAGGGGA GCTGGTCACC AGCACGTCTG TGGAGTCCAC GTCGTCAGCC 
ACTCTGTCTC AGCTCGAATC TTCCCTTGCA GAGGACTCCC ATCTTGGACC AAGTCAGAGC 
TCCTTCCATC AAGTTCCACA AGGTCCATCA CAATCAGTAG ACAGTTACCG ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTRL ( XM_006054145.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006054207.1 Bubalus bubalis centriolin (CNTRL), transcript variant X3, mRNA.

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CloneID	OBu11630
Clone ID Related Accession (Same CDS sequence)	XM_006054145.1
Accession Version	XM_006054145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	centriolin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784589.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCTAAGACA GAAGACCATG 
GAACTAACGC GAGCATGTCA AAAACAGTAT GAACTGGAAC AGGAGTTGGC TTTTTACAAA 
ATCGATGCTA AATTTGAGCC ACTGAATTAT TATCCCTCAG AGTATGTTGA CGTTGATAAA 
GCCCCAGATG AAAGCCCTTA CATTGGCAAG TCCAGATACA AGAGAAATAT GTTCGCCACA 
GAGAGTTACA TTGTGAATAA TGCCCAGCCA GTTGAGATCA AGAGGATGGA GCCAGAGGAA 
GGGGAGCAAC TTAGAAACGA GCATGTGAGC TTGAAAGCCC ATATGCCACT GGACACGGAG 
CTGGAAGACA AAGAGAACAA AATAAGTACA GCACAAGCTC GACTATCAGC ACTGCATGAT 
GAAATAGAAA AGGCAGAACA ACAAATTTTA AGAGCCACTG AAGAATTTAA ACAACTGGAA 
GAAGCTGTAC AACTTAAAAA GATTTCAGAA GCAGAGAAAG ACTTTCTTCT CAAGCAGCTG 
AGTGGCAGGA TACAGCTTCT AAATAAATTA CGCCAGGAAG CTCTTGATCT AGAAATGCAG 
ATGGAAAAGC AAAGAGGAGA AATTGCTGAA AAGCAGGAGG AAATCAAGGA CCTCCAAAGA 
GTTATTGATA GTCTGGATTC CAAAGACCCA AAACATTGCC ATATGAAGGC TCAGAAAAGG 
GGTAAAGAAC AACAATTTGA CATTATGAAC AAGCAGTACA AACAACTTGA GAGCCGTTTG 
GACGAGATAC TTTCTAGAAT TGCTAAAGAA ACTGAAGAGA TTAAGGACCT TGAACAACAG 
CTTACTGAAG GCCAGATAGC AGCAAATGAA GCCCTGAAGA AGGACTTAGA AGGCGTCATC 
AGTGGCCTGC AAGAATACCT GGGCACTGTG AAGGGCCAGG CCGCTCAGGC CCAGAGCGAG 
TGCAGAAGGC TGCAGGATGA GAAAGAGGCG CTGCTGCAGA GGTTGGCCGA GGTCGAGCAG 
GAAAGGGACC AGCTGGAAGC AGTCGCCTTA GATGCAGAAG CTATGAGGAA GGAGCTTGCA 
GAGCTAGAAA GCGCCCTCCA AGAGCAGCAT GAGGTGAACG CGTCCCTGCA GCAGACCCAC 
GGAGACCTCA GTGCCTACGA GGCTGAGCTA GAGGCACAGC TGAGGATGAG GGATGCCGAA 
GCCAGCCAGC TCAAAGAAGA GCTGGGAGAA CTAACAAGGC TCAGCCAGCT AGAACAGTCA 
ACTCTACAAA CAGAACTTGA GAAAGAAAAA CAAGCCCTCA AGAACGCCCT GGGAAAAGCC 
CAGCTCTCGG AAGAAAAAGA ACAAGAAAAC AGTGAGCTCC GCACCCAGCT TAAACAGCTA 
CAGGATGACA ATAACCTGTT AAAACAGCAA CTTAAAGATT TCCAGAATCA CCTTAATCAG 
GTAGCTGATG GTCTGATTCG TCCAGAAGAA GTGGCAGCTC GTGTAGATGA AGTAAGGAGA 
AAATTGAAAT CAGGCGCTGG AGAGATGAGC AGAATCCACA GTCCTTCAGA TGTCTTAGGG 
AAAAGTCTTG CTGACTTGCA GATACAATTC AGTGAAATGC TGACACGTTC TCAGTGGGAG 
AAAGAGGAAG CCCAAGGGAG GGAAAGAAAA CTCCAAGAAG AAATGGCTCT GCATCAGGAG 
AAGCTGGTGG ATGGACAGGA AGCGTTCAGG CAGGCCTGTG AGAGAGCGCT AGAAGCAAGG 
ATTAATTTTG ACAAGAGGCA ACATGAAGCA AGAATTCAGC AGTTAGAGAA TGAGATTCAC 
TATTTACAAG AAAATCTAAA AAGTATGGAG GAAATCCAAG GGCTTACAGA TCTGCAGCTG 
CAGGAAGCTG ATGAAGAGAA GGAGAGAATT CTAGCCCAAC TCCAGGAGTT AGAGAAGAAG 
AAGAAACTTG AAGATGCCAA ATCTCAGGCA CAGGTTCTAG GTTTAGATAA AGAATTGAAG 
AAACTAAAGA AGGCTGTGGC CACCTCTGAT AAGATAGCCA CAGCTGAGCT CACCATCGCC 
AAAGACCAGC TCAAGTCCCT TCATGGAACT GTTATGAAAA TTAACCAGGA GCGTGCAGAG 
GACCTGCAGG AAGCAGAGCG GTTCAGCAGA AAGGCAGCAC AGGCCGCGAG GGATCTGACC 
CGAGCGGAAG CGGAGATCGA ACTCCTGCAG AACCTCCTCA GGGAAAGAGA GGAGCAGATG 
GAGAAGGTAG AGGCAGGCAC CCGAGGAGCA AGCTCACAGG CGCTGGAAAT CGAGAGGTGG 
AATGAGACCA TGGAGAGACA GAGGAGCGAG ATCGCAAGGC TACGGGACTC ACTAGACCGC 
ACCGGGGCGG ATAACAGAGG AGGCATTGAA AATATTTTGG AAGAAATAGC TGAACTCCGA 
CGTGAAGTTT CTTACCAGAA TGATTACATC AGCAGCATGG CAGATCCTTT CAAAAGACGA 
GGCTATTGGT ACTTTATGCC ACCACCATCA TCATCAAAAG TTTCCAGCCA CAGCTCCCAG 
GCCACAAAGG ACTCTGGTGT CGGGATGAAG TACGCAGCCT CGACTCCTAT TAGAAAACCA 
CAGCAGAACG GGAGGGAAGA CGGTGCCCCT CCAGCTGCCT CCGGATACTG GGTGTATTCC 
CCCATCAGGA GCGGCTTACA TAAATCGTTT TCAAATAAAG ATGCAGACAG TGGAGGAGAG 
AGCCGGGAGG AGAGCGAGCT GGATGACCAC GATGACCGCC CGTTCGTCCC TCCTCCTGGA 
TACATGATGT ACACCGTGTT CCCCGACGGC TCTCCTGTAC CACAGGGCAT GGCCCTCTAC 
GCACCGCCGC CTCCCTTGCC CAACAATAGC CGACCTCTCA GCCCTGGCAC TGTTGTGTAT 
GGCCCGCCCC CTGCTGGTGC CCCCATTGTC TACGGGCCCC CACCGCCCAG CTTCTCCGTC 
CCCCTCATCC CAGTGGGCGT GCTGCACTGC AACGTCCCGG AGCACCATGC CTTAGAGAAT 
GAGGTTTCTA GATTGGAAGA CATAATACAG CATTTAAAAT CAAAGAAGCA GGAACAGAGG 
ATGAGAGTAT GCAAGCGGCA GTCTGAGAAA GAAATAGAAG AACTGCATCA TAATATTGAT 
GAACTTTTGC AAGAGAAGAA AGACTTAGAG CACGAAGTAG AAGAATTACA TAGAACCATC 
CAGAAACATC AACAGCGAAA AGACTTCATT GATGGAAATG TTGAGAGTCT TATGAATGAA 
CTAAAAATAG AAAAATCACT CAAGCACCAT GAAGATATTG TAGATGAAAT TGAGTGCATC 
GAGAAGACTC TCCTGAAACG TCGAGCAGAG CTCAGGGAGG CTGACCGACT GCTGGCGGAA 
GCCGAGAGTG AACTTTCACG CACAACAGAG AAAACAAAAA ATGCTGTTGA AAAGTTCACT 
GATGCCAAGA GGAATTTGTT GCAAACTGAG AAAGATGCTG AGGAGTTAGA AAGGAGAGCT 
CAGGAAACAG CTGTTAGCCT TGTCAAGGCT GATCAACAGC TCAGATTGTT ACAGGCGGAC 
ACAAAGGATT TGGAGCAGCA CAAAACTGAG CAAGAAGAGA TCTTGAAAGA GATAAACAAA 
GTTGTTGCAG CAAAAGACTC AGACTTCCAG TGTTTAAACA CGAAGAAGGA GAAGCTAACA 
GAAGAGCTGC AGAAGCTGCA GAAAGACATC GAGACGGCAG CGCGCACGGA GGATCACCAC 
CTGCAGGTCC TGAGAGAGTC GGAGGCCCTC CTGCAGGCCA AGAGAGCGGA GCTGGACAAG 
CTGAGGAGCC AGGTAACGAG TCAGCAGCAG GAGATGGCTG TCTTGGACAG ACAGCTGGGG 
CATAAGAAGG AGGAGCTGCA TCTACTCCAG GGAAGCATGG TCCAGGCCAG AGCTGACCTC 
CAGGAAGCGC TGAGGCTGGG AGAAACTGAA GTAACTGAGA AGTGCAACCA CATCAGGGAA 
CTAAAAGGTC TTCTGGAAGA ACTGAGTTTT CAGAAAGGAG AACTGAATGT CCAGATTAGT 
GAGAAAAAAA ATCAACTTTC ACTTATAAAG CAAGAAATTG AAAAAGAGGA AGAAAATCTT 
CAGGTAGTTT TAGGGCAGAT GTCTAAACAT AAAACTGAAC TTAAGAATAT TCTGGACATG 
TTGCAACTTG AAAACAATGA GCTGCAAGCT TTGAAGCTAC AGCATGAGCA AAAGATGTCT 
GAGTTAGAGA AGGCTCAGGT TGAAGTGCTG GAGGAGAAAC TGGAGTTGGA GAATTTGCAG 
CAGACAGCCC TGCGACAGAA AGGGGAGATC GAGTGGCAGA AGCAGCTCTT AGAGAGGGAC 
AAGCGAGAGA TTGAGCGGAT AACTGCTGAG ACCCGAGCAT TGCAGTTGTG TGTTGAGTCT 
TTGTGCAAAC AAAAGGAAGA TCTTGAGGAG AAATGTGACA GCTGGGAGAA GAAGCTGGCA 
CAGACCAAAC GGGTTTTAGC AGCCACAGAA GAGAATAGCA AAGCAAAGCA ATCAGACTTA 
GAAAAGTTGG AATTGGATAT CAGAAAACTG GAACAGGAAC TAGACCAGCT AAACAGAAAC 
AAACTCTCAC TGCACAGAGA CATCGCGGCC ATGCAGCAGC AGGTCCAAGA AAAACGAGAA 
GCAGTAAACT CATTACAGGA GGAACTAGCT GATGTCCAAG CCCATTTGAA CCTAGCGAAA 
CAGGACCTGC TCCACACCAC CAAGCGCCAG GATGTCCTGC TTGGCGAGCA GACCAGGCTG 
CAGGAGGACC TCCAGGAACG CAGAAAGAAG TTGGAAGTCT GCCAAAAAGA AGAGGCAACA 
AAGCGGCAGC ACCTGCAAGC GCTTCAGACG GAGATGGAGG CGAACGAGGC CGAGCTGGCC 
CGGCAGGAGA CGACATTTCA GAGACTCCAG AAAGAGAGAG AAAGTGAAGA AAAAAAGTTA 
GAAGCCAGTC GAGCGACTCT GCAGGAGCAG CAGCAGCAGC TGGAAAAGGA AATCGCAGAC 
CAGAAGAACG TGCTGGACCA GGTGCTCAGC CAGGTGCTGG GGGCCAAGGA GCGCGCCAGG 
ACTCTGCAGC AGGAGGAGAG GTGGGGTGAG ACCCTGGAGA AGACGCTCTC CCAGACCCAG 
CAACAGCTCT CAGAACGGGA GCAGCAGTTG TTGGAAAAGT CAGCTGAGCT GCTGGCTCTT 
CAGAAAGAGG CAGACTCGAT GAGGGCAGAC TTCAGCCTCT TGCGAAACCA GTTCTTGACG 
GAGAGGAAGA AAGCCGAGAA GCAGGTAGCC AGCCTCAGGG AAGCGCTCAA GACCCAGCGC 
AGCCAGCTGG AGAAGACCCT TCTCGAGCAG AAGCAGGAGA ACAGCTCCAT GCAGAAGGAG 
ATGGCGACTA TCGAGCAGGT GGCGCAGGAC AACCACGAGC GGGCCCGGCG CCTGATGCGG 
GAGCTCAGCC AGATGCAGCA GGAGTACGCG GAGCTCAAGA AGCAGATGGT AAACCAGAAA 
GATCTGGAAA GAAGACAAGT AGAAATCAGT GAAGCGATGA GGACACTTAA GTCTGAAGTG 
AAAGACGAGA TCAGAGCCAG CCTGAAGAAC CTCAACCAGT TTCTCCCGGA GCTGCCAGCA 
GACCTGGAGG CCATTCTGGA AAGGAGCGAA CACCTAGACG GAGGGCTGGA GAGCTTGAAG 
GAGAACTTTC CATTCGCCCT GAGTGAGAAG CCATCACCTT TGGAAGAGAA ACTACATTTT 
TCTCGCGTTC ACATAATGGC CCAGCTCCGA CACTGTATGT CCAAGCAGGC AGAGGTGTTA 
AGCAAAGGGA AGCGGCAGAC CGAGGGCACG TTACACAGCC TGAGGAGGCA GGTCGACGCC 
TTAGGGGAGC TGGTCACCAG CACGTCTGTG GAGTCCACGT CGTCAGCCAC TCTGTCTCAG 
CTCGAATCTT CCCTTGCAGA GGACTCCCAT CTTGGACCAA GTCAGAGCTC CTTCCATCAA 
GTTCCACAAG GTCCATCACA ATCAGTAGAC AGTTACCGAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006054207.1
CDS	129..7073
Translation	MKKGPQQKISKTKMPPSSHSPSPSSLTSNMRSRSLSPLNGSETLPFHSGRQWYGQVEIIGENSMLLDYHDQKEADSHAGVRYITEALVKKLTKQENLTLVKSLNLSLSKDGGKKFKYIENLEKCTKLEILNLSHNLIGKIEKVDKLLKLRELNLSYNKICKIEGIENMHNLQKLNLAGNEIEHIPAWLGKKLKCLRVLNLKANKISSLQDVSKLKPLQDLTSLILAENPVVTLPHYLQFTIFHLRSLESLEGQPVTTQDRQEAFERFSLEEVERLERDLEKKMMETEELKSKQAQFLEEIKNQDKLNRSLKEEAMLQKQSCEELESNLNTKNELLRQKTMELTRACQKQYELEQELAFYKIDAKFEPLNYYPSEYVDVDKAPDESPYIGKSRYKRNMFATESYIVNNAQPVEIKRMEPEEGEQLRNEHVSLKAHMPLDTELEDKENKISTAQARLSALHDEIEKAEQQILRATEEFKQLEEAVQLKKISEAEKDFLLKQLSGRIQLLNKLRQEALDLEMQMEKQRGEIAEKQEEIKDLQRVIDSLDSKDPKHCHMKAQKRGKEQQFDIMNKQYKQLESRLDEILSRIAKETEEIKDLEQQLTEGQIAANEALKKDLEGVISGLQEYLGTVKGQAAQAQSECRRLQDEKEALLQRLAEVEQERDQLEAVALDAEAMRKELAELESALQEQHEVNASLQQTHGDLSAYEAELEAQLRMRDAEASQLKEELGELTRLSQLEQSTLQTELEKEKQALKNALGKAQLSEEKEQENSELRTQLKQLQDDNNLLKQQLKDFQNHLNQVADGLIRPEEVAARVDEVRRKLKSGAGEMSRIHSPSDVLGKSLADLQIQFSEMLTRSQWEKEEAQGRERKLQEEMALHQEKLVDGQEAFRQACERALEARINFDKRQHEARIQQLENEIHYLQENLKSMEEIQGLTDLQLQEADEEKERILAQLQELEKKKKLEDAKSQAQVLGLDKELKKLKKAVATSDKIATAELTIAKDQLKSLHGTVMKINQERAEDLQEAERFSRKAAQAARDLTRAEAEIELLQNLLREREEQMEKVEAGTRGASSQALEIERWNETMERQRSEIARLRDSLDRTGADNRGGIENILEEIAELRREVSYQNDYISSMADPFKRRGYWYFMPPPSSSKVSSHSSQATKDSGVGMKYAASTPIRKPQQNGREDGAPPAASGYWVYSPIRSGLHKSFSNKDADSGGESREESELDDHDDRPFVPPPGYMMYTVFPDGSPVPQGMALYAPPPPLPNNSRPLSPGTVVYGPPPAGAPIVYGPPPPSFSVPLIPVGVLHCNVPEHHALENEVSRLEDIIQHLKSKKQEQRMRVCKRQSEKEIEELHHNIDELLQEKKDLEHEVEELHRTIQKHQQRKDFIDGNVESLMNELKIEKSLKHHEDIVDEIECIEKTLLKRRAELREADRLLAEAESELSRTTEKTKNAVEKFTDAKRNLLQTEKDAEELERRAQETAVSLVKADQQLRLLQADTKDLEQHKTEQEEILKEINKVVAAKDSDFQCLNTKKEKLTEELQKLQKDIETAARTEDHHLQVLRESEALLQAKRAELDKLRSQVTSQQQEMAVLDRQLGHKKEELHLLQGSMVQARADLQEALRLGETEVTEKCNHIRELKGLLEELSFQKGELNVQISEKKNQLSLIKQEIEKEEENLQVVLGQMSKHKTELKNILDMLQLENNELQALKLQHEQKMSELEKAQVEVLEEKLELENLQQTALRQKGEIEWQKQLLERDKREIERITAETRALQLCVESLCKQKEDLEEKCDSWEKKLAQTKRVLAATEENSKAKQSDLEKLELDIRKLEQELDQLNRNKLSLHRDIAAMQQQVQEKREAVNSLQEELADVQAHLNLAKQDLLHTTKRQDVLLGEQTRLQEDLQERRKKLEVCQKEEATKRQHLQALQTEMEANEAELARQETTFQRLQKERESEEKKLEASRATLQEQQQQLEKEIADQKNVLDQVLSQVLGAKERARTLQQEERWGETLEKTLSQTQQQLSEREQQLLEKSAELLALQKEADSMRADFSLLRNQFLTERKKAEKQVASLREALKTQRSQLEKTLLEQKQENSSMQKEMATIEQVAQDNHERARRLMRELSQMQQEYAELKKQMVNQKDLERRQVEISEAMRTLKSEVKDEIRASLKNLNQFLPELPADLEAILERSEHLDGGLESLKENFPFALSEKPSPLEEKLHFSRVHIMAQLRHCMSKQAEVLSKGKRQTEGTLHSLRRQVDALGELVTSTSVESTSSATLSQLESSLAEDSHLGPSQSSFHQVPQGPSQSVDSYRH

Target ORF information:

RefSeq Version	XM_006054145.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centriolin (CNTRL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006054145.1

ORF Insert Sequence:

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCTAAGACA GAAGACCATG 
GAACTAACGC GAGCATGTCA AAAACAGTAT GAACTGGAAC AGGAGTTGGC TTTTTACAAA 
ATCGATGCTA AATTTGAGCC ACTGAATTAT TATCCCTCAG AGTATGTTGA CGTTGATAAA 
GCCCCAGATG AAAGCCCTTA CATTGGCAAG TCCAGATACA AGAGAAATAT GTTCGCCACA 
GAGAGTTACA TTGTGAATAA TGCCCAGCCA GTTGAGATCA AGAGGATGGA GCCAGAGGAA 
GGGGAGCAAC TTAGAAACGA GCATGTGAGC TTGAAAGCCC ATATGCCACT GGACACGGAG 
CTGGAAGACA AAGAGAACAA AATAAGTACA GCACAAGCTC GACTATCAGC ACTGCATGAT 
GAAATAGAAA AGGCAGAACA ACAAATTTTA AGAGCCACTG AAGAATTTAA ACAACTGGAA 
GAAGCTGTAC AACTTAAAAA GATTTCAGAA GCAGAGAAAG ACTTTCTTCT CAAGCAGCTG 
AGTGGCAGGA TACAGCTTCT AAATAAATTA CGCCAGGAAG CTCTTGATCT AGAAATGCAG 
ATGGAAAAGC AAAGAGGAGA AATTGCTGAA AAGCAGGAGG AAATCAAGGA CCTCCAAAGA 
GTTATTGATA GTCTGGATTC CAAAGACCCA AAACATTGCC ATATGAAGGC TCAGAAAAGG 
GGTAAAGAAC AACAATTTGA CATTATGAAC AAGCAGTACA AACAACTTGA GAGCCGTTTG 
GACGAGATAC TTTCTAGAAT TGCTAAAGAA ACTGAAGAGA TTAAGGACCT TGAACAACAG 
CTTACTGAAG GCCAGATAGC AGCAAATGAA GCCCTGAAGA AGGACTTAGA AGGCGTCATC 
AGTGGCCTGC AAGAATACCT GGGCACTGTG AAGGGCCAGG CCGCTCAGGC CCAGAGCGAG 
TGCAGAAGGC TGCAGGATGA GAAAGAGGCG CTGCTGCAGA GGTTGGCCGA GGTCGAGCAG 
GAAAGGGACC AGCTGGAAGC AGTCGCCTTA GATGCAGAAG CTATGAGGAA GGAGCTTGCA 
GAGCTAGAAA GCGCCCTCCA AGAGCAGCAT GAGGTGAACG CGTCCCTGCA GCAGACCCAC 
GGAGACCTCA GTGCCTACGA GGCTGAGCTA GAGGCACAGC TGAGGATGAG GGATGCCGAA 
GCCAGCCAGC TCAAAGAAGA GCTGGGAGAA CTAACAAGGC TCAGCCAGCT AGAACAGTCA 
ACTCTACAAA CAGAACTTGA GAAAGAAAAA CAAGCCCTCA AGAACGCCCT GGGAAAAGCC 
CAGCTCTCGG AAGAAAAAGA ACAAGAAAAC AGTGAGCTCC GCACCCAGCT TAAACAGCTA 
CAGGATGACA ATAACCTGTT AAAACAGCAA CTTAAAGATT TCCAGAATCA CCTTAATCAG 
GTAGCTGATG GTCTGATTCG TCCAGAAGAA GTGGCAGCTC GTGTAGATGA AGTAAGGAGA 
AAATTGAAAT CAGGCGCTGG AGAGATGAGC AGAATCCACA GTCCTTCAGA TGTCTTAGGG 
AAAAGTCTTG CTGACTTGCA GATACAATTC AGTGAAATGC TGACACGTTC TCAGTGGGAG 
AAAGAGGAAG CCCAAGGGAG GGAAAGAAAA CTCCAAGAAG AAATGGCTCT GCATCAGGAG 
AAGCTGGTGG ATGGACAGGA AGCGTTCAGG CAGGCCTGTG AGAGAGCGCT AGAAGCAAGG 
ATTAATTTTG ACAAGAGGCA ACATGAAGCA AGAATTCAGC AGTTAGAGAA TGAGATTCAC 
TATTTACAAG AAAATCTAAA AAGTATGGAG GAAATCCAAG GGCTTACAGA TCTGCAGCTG 
CAGGAAGCTG ATGAAGAGAA GGAGAGAATT CTAGCCCAAC TCCAGGAGTT AGAGAAGAAG 
AAGAAACTTG AAGATGCCAA ATCTCAGGCA CAGGTTCTAG GTTTAGATAA AGAATTGAAG 
AAACTAAAGA AGGCTGTGGC CACCTCTGAT AAGATAGCCA CAGCTGAGCT CACCATCGCC 
AAAGACCAGC TCAAGTCCCT TCATGGAACT GTTATGAAAA TTAACCAGGA GCGTGCAGAG 
GACCTGCAGG AAGCAGAGCG GTTCAGCAGA AAGGCAGCAC AGGCCGCGAG GGATCTGACC 
CGAGCGGAAG CGGAGATCGA ACTCCTGCAG AACCTCCTCA GGGAAAGAGA GGAGCAGATG 
GAGAAGGTAG AGGCAGGCAC CCGAGGAGCA AGCTCACAGG CGCTGGAAAT CGAGAGGTGG 
AATGAGACCA TGGAGAGACA GAGGAGCGAG ATCGCAAGGC TACGGGACTC ACTAGACCGC 
ACCGGGGCGG ATAACAGAGG AGGCATTGAA AATATTTTGG AAGAAATAGC TGAACTCCGA 
CGTGAAGTTT CTTACCAGAA TGATTACATC AGCAGCATGG CAGATCCTTT CAAAAGACGA 
GGCTATTGGT ACTTTATGCC ACCACCATCA TCATCAAAAG TTTCCAGCCA CAGCTCCCAG 
GCCACAAAGG ACTCTGGTGT CGGGATGAAG TACGCAGCCT CGACTCCTAT TAGAAAACCA 
CAGCAGAACG GGAGGGAAGA CGGTGCCCCT CCAGCTGCCT CCGGATACTG GGTGTATTCC 
CCCATCAGGA GCGGCTTACA TAAATCGTTT TCAAATAAAG ATGCAGACAG TGGAGGAGAG 
AGCCGGGAGG AGAGCGAGCT GGATGACCAC GATGACCGCC CGTTCGTCCC TCCTCCTGGA 
TACATGATGT ACACCGTGTT CCCCGACGGC TCTCCTGTAC CACAGGGCAT GGCCCTCTAC 
GCACCGCCGC CTCCCTTGCC CAACAATAGC CGACCTCTCA GCCCTGGCAC TGTTGTGTAT 
GGCCCGCCCC CTGCTGGTGC CCCCATTGTC TACGGGCCCC CACCGCCCAG CTTCTCCGTC 
CCCCTCATCC CAGTGGGCGT GCTGCACTGC AACGTCCCGG AGCACCATGC CTTAGAGAAT 
GAGGTTTCTA GATTGGAAGA CATAATACAG CATTTAAAAT CAAAGAAGCA GGAACAGAGG 
ATGAGAGTAT GCAAGCGGCA GTCTGAGAAA GAAATAGAAG AACTGCATCA TAATATTGAT 
GAACTTTTGC AAGAGAAGAA AGACTTAGAG CACGAAGTAG AAGAATTACA TAGAACCATC 
CAGAAACATC AACAGCGAAA AGACTTCATT GATGGAAATG TTGAGAGTCT TATGAATGAA 
CTAAAAATAG AAAAATCACT CAAGCACCAT GAAGATATTG TAGATGAAAT TGAGTGCATC 
GAGAAGACTC TCCTGAAACG TCGAGCAGAG CTCAGGGAGG CTGACCGACT GCTGGCGGAA 
GCCGAGAGTG AACTTTCACG CACAACAGAG AAAACAAAAA ATGCTGTTGA AAAGTTCACT 
GATGCCAAGA GGAATTTGTT GCAAACTGAG AAAGATGCTG AGGAGTTAGA AAGGAGAGCT 
CAGGAAACAG CTGTTAGCCT TGTCAAGGCT GATCAACAGC TCAGATTGTT ACAGGCGGAC 
ACAAAGGATT TGGAGCAGCA CAAAACTGAG CAAGAAGAGA TCTTGAAAGA GATAAACAAA 
GTTGTTGCAG CAAAAGACTC AGACTTCCAG TGTTTAAACA CGAAGAAGGA GAAGCTAACA 
GAAGAGCTGC AGAAGCTGCA GAAAGACATC GAGACGGCAG CGCGCACGGA GGATCACCAC 
CTGCAGGTCC TGAGAGAGTC GGAGGCCCTC CTGCAGGCCA AGAGAGCGGA GCTGGACAAG 
CTGAGGAGCC AGGTAACGAG TCAGCAGCAG GAGATGGCTG TCTTGGACAG ACAGCTGGGG 
CATAAGAAGG AGGAGCTGCA TCTACTCCAG GGAAGCATGG TCCAGGCCAG AGCTGACCTC 
CAGGAAGCGC TGAGGCTGGG AGAAACTGAA GTAACTGAGA AGTGCAACCA CATCAGGGAA 
CTAAAAGGTC TTCTGGAAGA ACTGAGTTTT CAGAAAGGAG AACTGAATGT CCAGATTAGT 
GAGAAAAAAA ATCAACTTTC ACTTATAAAG CAAGAAATTG AAAAAGAGGA AGAAAATCTT 
CAGGTAGTTT TAGGGCAGAT GTCTAAACAT AAAACTGAAC TTAAGAATAT TCTGGACATG 
TTGCAACTTG AAAACAATGA GCTGCAAGCT TTGAAGCTAC AGCATGAGCA AAAGATGTCT 
GAGTTAGAGA AGGCTCAGGT TGAAGTGCTG GAGGAGAAAC TGGAGTTGGA GAATTTGCAG 
CAGACAGCCC TGCGACAGAA AGGGGAGATC GAGTGGCAGA AGCAGCTCTT AGAGAGGGAC 
AAGCGAGAGA TTGAGCGGAT AACTGCTGAG ACCCGAGCAT TGCAGTTGTG TGTTGAGTCT 
TTGTGCAAAC AAAAGGAAGA TCTTGAGGAG AAATGTGACA GCTGGGAGAA GAAGCTGGCA 
CAGACCAAAC GGGTTTTAGC AGCCACAGAA GAGAATAGCA AAGCAAAGCA ATCAGACTTA 
GAAAAGTTGG AATTGGATAT CAGAAAACTG GAACAGGAAC TAGACCAGCT AAACAGAAAC 
AAACTCTCAC TGCACAGAGA CATCGCGGCC ATGCAGCAGC AGGTCCAAGA AAAACGAGAA 
GCAGTAAACT CATTACAGGA GGAACTAGCT GATGTCCAAG CCCATTTGAA CCTAGCGAAA 
CAGGACCTGC TCCACACCAC CAAGCGCCAG GATGTCCTGC TTGGCGAGCA GACCAGGCTG 
CAGGAGGACC TCCAGGAACG CAGAAAGAAG TTGGAAGTCT GCCAAAAAGA AGAGGCAACA 
AAGCGGCAGC ACCTGCAAGC GCTTCAGACG GAGATGGAGG CGAACGAGGC CGAGCTGGCC 
CGGCAGGAGA CGACATTTCA GAGACTCCAG AAAGAGAGAG AAAGTGAAGA AAAAAAGTTA 
GAAGCCAGTC GAGCGACTCT GCAGGAGCAG CAGCAGCAGC TGGAAAAGGA AATCGCAGAC 
CAGAAGAACG TGCTGGACCA GGTGCTCAGC CAGGTGCTGG GGGCCAAGGA GCGCGCCAGG 
ACTCTGCAGC AGGAGGAGAG GTGGGGTGAG ACCCTGGAGA AGACGCTCTC CCAGACCCAG 
CAACAGCTCT CAGAACGGGA GCAGCAGTTG TTGGAAAAGT CAGCTGAGCT GCTGGCTCTT 
CAGAAAGAGG CAGACTCGAT GAGGGCAGAC TTCAGCCTCT TGCGAAACCA GTTCTTGACG 
GAGAGGAAGA AAGCCGAGAA GCAGGTAGCC AGCCTCAGGG AAGCGCTCAA GACCCAGCGC 
AGCCAGCTGG AGAAGACCCT TCTCGAGCAG AAGCAGGAGA ACAGCTCCAT GCAGAAGGAG 
ATGGCGACTA TCGAGCAGGT GGCGCAGGAC AACCACGAGC GGGCCCGGCG CCTGATGCGG 
GAGCTCAGCC AGATGCAGCA GGAGTACGCG GAGCTCAAGA AGCAGATGGT AAACCAGAAA 
GATCTGGAAA GAAGACAAGT AGAAATCAGT GAAGCGATGA GGACACTTAA GTCTGAAGTG 
AAAGACGAGA TCAGAGCCAG CCTGAAGAAC CTCAACCAGT TTCTCCCGGA GCTGCCAGCA 
GACCTGGAGG CCATTCTGGA AAGGAGCGAA CACCTAGACG GAGGGCTGGA GAGCTTGAAG 
GAGAACTTTC CATTCGCCCT GAGTGAGAAG CCATCACCTT TGGAAGAGAA ACTACATTTT 
TCTCGCGTTC ACATAATGGC CCAGCTCCGA CACTGTATGT CCAAGCAGGC AGAGGTGTTA 
AGCAAAGGGA AGCGGCAGAC CGAGGGCACG TTACACAGCC TGAGGAGGCA GGTCGACGCC 
TTAGGGGAGC TGGTCACCAG CACGTCTGTG GAGTCCACGT CGTCAGCCAC TCTGTCTCAG 
CTCGAATCTT CCCTTGCAGA GGACTCCCAT CTTGGACCAA GTCAGAGCTC CTTCCATCAA 
GTTCCACAAG GTCCATCACA ATCAGTAGAC AGTTACCGAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTRL ( XM_006054146.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006054208.1 Bubalus bubalis centriolin (CNTRL), transcript variant X4, mRNA.

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CloneID	OBu11631
Clone ID Related Accession (Same CDS sequence)	XM_006054146.1
Accession Version	XM_006054146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	centriolin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784589.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATAG AGGGCGAGCT GGAAGGCTAT GGGCTTAGGA ATGTTGGCGG CCTGTATTTA 
GATGATGGAT GTGGAACAGG CAGGCCCACT GTGGAAGTGG GGTCAGCAGC GCTTTCTAAT 
GGATTACATA ATCCTCAGGC TTCTGGCTTA AAAGAGGTAG AAAGACTGGA AAGAGACCTG 
GAGAAGAAGA TGATGGAAAC TGAAGAGCTT AAAAGCAAAC AAGCACAGTT CCTTGAAGAA 
ATTAAAAATC AAGATAAGTT GAACAGATCA TTAAAAGAGG AGGCTATGCT ACAAAAACAG 
AGCTGTGAGG AGCTCGAGAG TAACCTTAAC ACGAAAAATG AACTGCTAAG ACAGAAGACC 
ATGGAACTAA CGCGAGCATG TCAAAAACAG TATGAACTGG AACAGGAGTT GGCTTTTTAC 
AAAATCGATG CTAAATTTGA GCCACTGAAT TATTATCCCT CAGAGTATGT TGACGTTGAT 
AAAGCCCCAG ATGAAAGCCC TTACATTGGC AAGTCCAGAT ACAAGAGAAA TATGTTCGCC 
ACAGAGAGTT ACATTGTGAA TAATGCCCAG CCAGTTGAGA TCAAGAGGAT GGAGCCAGAG 
GAAGGGGAGC AACTTAGAAA CGAGCATGTG AGCTTGAAAG CCCATATGCC ACTGGACACG 
GAGCTGGAAG ACAAAGAGAA CAAAATAAGT ACAGCACAAG CTCGACTATC AGCACTGCAT 
GATGAAATAG AAAAGGCAGA ACAACAAATT TTAAGAGCCA CTGAAGAATT TAAACAACTG 
GAAGAAGCTG TACAACTTAA AAAGATTTCA GAAGCAGAGA AAGACTTTCT TCTCAAGCAG 
CTGAGTGGCA GGATACAGCT TCTAAATAAA TTACGCCAGG AAGCTCTTGA TCTAGAAATG 
CAGATGGAAA AGCAAAGAGG AGAAATTGCT GAAAAGCAGG AGGAAATCAA GGACCTCCAA 
AGAGTTATTG ATAGTCTGGA TTCCAAAGAC CCAAAACATT GCCATATGAA GGCTCAGAAA 
AGGGGTAAAG AACAACAATT TGACATTATG AACAAGCAGT ACAAACAACT TGAGAGCCGT 
TTGGACGAGA TACTTTCTAG AATTGCTAAA GAAACTGAAG AGATTAAGGA CCTTGAACAA 
CAGCTTACTG AAGGCCAGAT AGCAGCAAAT GAAGCCCTGA AGAAGGACTT AGAAGGCGTC 
ATCAGTGGCC TGCAAGAATA CCTGGGCACT GTGAAGGGCC AGGCCGCTCA GGCCCAGAGC 
GAGTGCAGAA GGCTGCAGGA TGAGAAAGAG GCGCTGCTGC AGAGGTTGGC CGAGGTCGAG 
CAGGAAAGGG ACCAGCTGGA AGCAGTCGCC TTAGATGCAG AAGCTATGAG GAAGGAGCTT 
GCAGAGCTAG AAAGCGCCCT CCAAGAGCAG CATGAGGTGA ACGCGTCCCT GCAGCAGACC 
CACGGAGACC TCAGTGCCTA CGAGGCTGAG CTAGAGGCAC AGCTGAGGAT GAGGGATGCC 
GAAGCCAGCC AGCTCAAAGA AGAGCTGGGA GAACTAACAA GGCTCAGCCA GCTAGAACAG 
TCAACTCTAC AAACAGAACT TGAGAAAGAA AAACAAGCCC TCAAGAACGC CCTGGGAAAA 
GCCCAGCTCT CGGAAGAAAA AGAACAAGAA AACAGTGAGC TCCGCACCCA GCTTAAACAG 
CTACAGGATG ACAATAACCT GTTAAAACAG CAACTTAAAG ATTTCCAGAA TCACCTTAAT 
CAGGTAGCTG ATGGTCTGAT TCGTCCAGAA GAAGTGGCAG CTCGTGTAGA TGAAGTAAGG 
AGAAAATTGA AATCAGGCGC TGGAGAGATG AGCAGAATCC ACAGTCCTTC AGATGTCTTA 
GGGAAAAGTC TTGCTGACTT GCAGATACAA TTCAGTGAAA TGCTGACACG TTCTCAGTGG 
GAGAAAGAGG AAGCCCAAGG GAGGGAAAGA AAACTCCAAG AAGAAATGGC TCTGCATCAG 
GAGAAGCTGG TGGATGGACA GGAAGCGTTC AGGCAGGCCT GTGAGAGAGC GCTAGAAGCA 
AGGATTAATT TTGACAAGAG GCAACATGAA GCAAGAATTC AGCAGTTAGA GAATGAGATT 
CACTATTTAC AAGAAAATCT AAAAAGTATG GAGGAAATCC AAGGGCTTAC AGATCTGCAG 
CTGCAGGAAG CTGATGAAGA GAAGGAGAGA ATTCTAGCCC AACTCCAGGA GTTAGAGAAG 
AAGAAGAAAC TTGAAGATGC CAAATCTCAG GCACAGGTTC TAGGTTTAGA TAAAGAATTG 
AAGAAACTAA AGAAGGCTGT GGCCACCTCT GATAAGATAG CCACAGCTGA GCTCACCATC 
GCCAAAGACC AGCTCAAGTC CCTTCATGGA ACTGTTATGA AAATTAACCA GGAGCGTGCA 
GAGGACCTGC AGGAAGCAGA GCGGTTCAGC AGAAAGGCAG CACAGGCCGC GAGGGATCTG 
ACCCGAGCGG AAGCGGAGAT CGAACTCCTG CAGAACCTCC TCAGGGAAAG AGAGGAGCAG 
ATGGAGAAGG TAGAGGCAGG CACCCGAGGA GCAAGCTCAC AGGCGCTGGA AATCGAGAGG 
TGGAATGAGA CCATGGAGAG ACAGAGGAGC GAGATCGCAA GGCTACGGGA CTCACTAGAC 
CGCACCGGGG CGGATAACAG AGGAGGCATT GAAAATATTT TGGAAGAAAT AGCTGAACTC 
CGACGTGAAG TTTCTTACCA GAATGATTAC ATCAGCAGCA TGGCAGATCC TTTCAAAAGA 
CGAGGCTATT GGTACTTTAT GCCACCACCA TCATCATCAA AAGTTTCCAG CCACAGCTCC 
CAGGCCACAA AGGACTCTGG TGTCGGGATG AAGTACGCAG CCTCGACTCC TATTAGAAAA 
CCACAGCAGA ACGGGAGGGA AGACGGTGCC CCTCCAGCTG CCTCCGGATA CTGGGTGTAT 
TCCCCCATCA GGAGCGGCTT ACATAAATCG TTTTCAAATA AAGATGCAGA CAGTGGAGGA 
GAGAGCCGGG AGGAGAGCGA GCTGGATGAC CACGATGACC GCCCGTTCGT CCCTCCTCCT 
GGATACATGA TGTACACCGT GTTCCCCGAC GGCTCTCCTG TACCACAGGG CATGGCCCTC 
TACGCACCGC CGCCTCCCTT GCCCAACAAT AGCCGACCTC TCAGCCCTGG CACTGTTGTG 
TATGGCCCGC CCCCTGCTGG TGCCCCCATT GTCTACGGGC CCCCACCGCC CAGCTTCTCC 
GTCCCCCTCA TCCCAGTGGG CGTGCTGCAC TGCAACGTCC CGGAGCACCA TGCCTTAGAG 
AATGAGGTTT CTAGATTGGA AGACATAATA CAGCATTTAA AATCAAAGAA GCAGGAACAG 
AGGATGAGAG TATGCAAGCG GCAGTCTGAG AAAGAAATAG AAGAACTGCA TCATAATATT 
GATGAACTTT TGCAAGAGAA GAAAGACTTA GAGCACGAAG TAGAAGAATT ACATAGAACC 
ATCCAGAAAC ATCAACAGCG AAAAGACTTC ATTGATGGAA ATGTTGAGAG TCTTATGAAT 
GAACTAAAAA TAGAAAAATC ACTCAAGCAC CATGAAGATA TTGTAGATGA AATTGAGTGC 
ATCGAGAAGA CTCTCCTGAA ACGTCGAGCA GAGCTCAGGG AGGCTGACCG ACTGCTGGCG 
GAAGCCGAGA GTGAACTTTC ACGCACAACA GAGAAAACAA AAAATGCTGT TGAAAAGTTC 
ACTGATGCCA AGAGGAATTT GTTGCAAACT GAGAAAGATG CTGAGGAGTT AGAAAGGAGA 
GCTCAGGAAA CAGCTGTTAG CCTTGTCAAG GCTGATCAAC AGCTCAGATT GTTACAGGCG 
GACACAAAGG ATTTGGAGCA GCACAAAACT GAGCAAGAAG AGATCTTGAA AGAGATAAAC 
AAAGTTGTTG CAGCAAAAGA CTCAGACTTC CAGTGTTTAA ACACGAAGAA GGAGAAGCTA 
ACAGAAGAGC TGCAGAAGCT GCAGAAAGAC ATCGAGACGG CAGCGCGCAC GGAGGATCAC 
CACCTGCAGG TCCTGAGAGA GTCGGAGGCC CTCCTGCAGG CCAAGAGAGC GGAGCTGGAC 
AAGCTGAGGA GCCAGGTAAC GAGTCAGCAG CAGGAGATGG CTGTCTTGGA CAGACAGCTG 
GGGCATAAGA AGGAGGAGCT GCATCTACTC CAGGGAAGCA TGGTCCAGGC CAGAGCTGAC 
CTCCAGGAAG CGCTGAGGCT GGGAGAAACT GAAGTAACTG AGAAGTGCAA CCACATCAGG 
GAACTAAAAG GTCTTCTGGA AGAACTGAGT TTTCAGAAAG GAGAACTGAA TGTCCAGATT 
AGTGAGAAAA AAAATCAACT TTCACTTATA AAGCAAGAAA TTGAAAAAGA GGAAGAAAAT 
CTTCAGGTAG TTTTAGGGCA GATGTCTAAA CATAAAACTG AACTTAAGAA TATTCTGGAC 
ATGTTGCAAC TTGAAAACAA TGAGCTGCAA GCTTTGAAGC TACAGCATGA GCAAAAGATG 
TCTGAGTTAG AGAAGGCTCA GGTTGAAGTG CTGGAGGAGA AACTGGAGTT GGAGAATTTG 
CAGCAGACAG CCCTGCGACA GAAAGGGGAG ATCGAGTGGC AGAAGCAGCT CTTAGAGAGG 
GACAAGCGAG AGATTGAGCG GATAACTGCT GAGACCCGAG CATTGCAGTT GTGTGTTGAG 
TCTTTGTGCA AACAAAAGGA AGATCTTGAG GAGAAATGTG ACAGCTGGGA GAAGAAGCTG 
GCACAGACCA AACGGGTTTT AGCAGCCACA GAAGAGAATA GCAAAGCAAA GCAATCAGAC 
TTAGAAAAGT TGGAATTGGA TATCAGAAAA CTGGAACAGG AACTAGACCA GCTAAACAGA 
AACAAACTCT CACTGCACAG AGACATCGCG GCCATGCAGC AGCAGGTCCA AGAAAAACGA 
GAAGCAGTAA ACTCATTACA GGAGGAACTA GCTGATGTCC AAGCCCATTT GAACCTAGCG 
AAACAGGACC TGCTCCACAC CACCAAGCGC CAGGATGTCC TGCTTGGCGA GCAGACCAGG 
CTGCAGGAGG ACCTCCAGGA ACGCAGAAAG AAGTTGGAAG TCTGCCAAAA AGAAGAGGCA 
ACAAAGCGGC AGCACCTGCA AGCGCTTCAG ACGGAGATGG AGGCGAACGA GGCCGAGCTG 
GCCCGGCAGG AGACGACATT TCAGAGACTC CAGAAAGAGA GAGAAAGTGA AGAAAAAAAG 
TTAGAAGCCA GTCGAGCGAC TCTGCAGGAG CAGCAGCAGC AGCTGGAAAA GGAAATCGCA 
GACCAGAAGA ACGTGCTGGA CCAGGTGCTC AGCCAGGTGC TGGGGGCCAA GGAGCGCGCC 
AGGACTCTGC AGCAGGAGGA GAGGTGGGGT GAGACCCTGG AGAAGACGCT CTCCCAGACC 
CAGCAACAGC TCTCAGAACG GGAGCAGCAG TTGTTGGAAA AGTCAGCTGA GCTGCTGGCT 
CTTCAGAAAG AGGCAGACTC GATGAGGGCA GACTTCAGCC TCTTGCGAAA CCAGTTCTTG 
ACGGAGAGGA AGAAAGCCGA GAAGCAGGTA GCCAGCCTCA GGGAAGCGCT CAAGACCCAG 
CGCAGCCAGC TGGAGAAGAC CCTTCTCGAG CAGAAGCAGG AGAACAGCTC CATGCAGAAG 
GAGATGGCGA CTATCGAGCA GGTGGCGCAG GACAACCACG AGCGGGCCCG GCGCCTGATG 
CGGGAGCTCA GCCAGATGCA GCAGGAGTAC GCGGAGCTCA AGAAGCAGAT GGTAAACCAG 
AAAGATCTGG AAAGAAGACA AGTAGAAATC AGTGAAGCGA TGAGGACACT TAAGTCTGAA 
GTGAAAGACG AGATCAGAGC CAGCCTGAAG AACCTCAACC AGTTTCTCCC GGAGCTGCCA 
GCAGACCTGG AGGCCATTCT GGAAAGGAGC GAACACCTAG ACGGAGGGCT GGAGAGCTTG 
AAGGAGAACT TTCCATTCGC CCTGAGTGAG AAGCCATCAC CTTTGGAAGA GAAACTACAT 
TTTTCTCGCG TTCACATAAT GGATGAGCAC TGGCGTGGAG AAGCGCTCCG GGAGAAGCTG 
CGGCACCGTG AGGACCGACT CAAGGCCCAG CTCCGACACT GTATGTCCAA GCAGGCAGAG 
GTGTTAAGCA AAGGGAAGCG GCAGACCGAG GGCACGTTAC ACAGCCTGAG GAGGCAGGTC 
GACGCCTTAG GGGAGCTGGT CACCAGCACG TCTGTGGAGT CCACGTCGTC AGCCACTCTG 
TCTCAGCTCG AATCTTCCCT TGCAGAGGAC TCCCATCTTG GACCAAGTCA GAGCTCCTTC 
CATCAAGTTC CACAAGGTCC ATCACAATCA GTAGACAGTT ACCGACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006054208.1
CDS	451..6801
Translation	MLIEGELEGYGLRNVGGLYLDDGCGTGRPTVEVGSAALSNGLHNPQASGLKEVERLERDLEKKMMETEELKSKQAQFLEEIKNQDKLNRSLKEEAMLQKQSCEELESNLNTKNELLRQKTMELTRACQKQYELEQELAFYKIDAKFEPLNYYPSEYVDVDKAPDESPYIGKSRYKRNMFATESYIVNNAQPVEIKRMEPEEGEQLRNEHVSLKAHMPLDTELEDKENKISTAQARLSALHDEIEKAEQQILRATEEFKQLEEAVQLKKISEAEKDFLLKQLSGRIQLLNKLRQEALDLEMQMEKQRGEIAEKQEEIKDLQRVIDSLDSKDPKHCHMKAQKRGKEQQFDIMNKQYKQLESRLDEILSRIAKETEEIKDLEQQLTEGQIAANEALKKDLEGVISGLQEYLGTVKGQAAQAQSECRRLQDEKEALLQRLAEVEQERDQLEAVALDAEAMRKELAELESALQEQHEVNASLQQTHGDLSAYEAELEAQLRMRDAEASQLKEELGELTRLSQLEQSTLQTELEKEKQALKNALGKAQLSEEKEQENSELRTQLKQLQDDNNLLKQQLKDFQNHLNQVADGLIRPEEVAARVDEVRRKLKSGAGEMSRIHSPSDVLGKSLADLQIQFSEMLTRSQWEKEEAQGRERKLQEEMALHQEKLVDGQEAFRQACERALEARINFDKRQHEARIQQLENEIHYLQENLKSMEEIQGLTDLQLQEADEEKERILAQLQELEKKKKLEDAKSQAQVLGLDKELKKLKKAVATSDKIATAELTIAKDQLKSLHGTVMKINQERAEDLQEAERFSRKAAQAARDLTRAEAEIELLQNLLREREEQMEKVEAGTRGASSQALEIERWNETMERQRSEIARLRDSLDRTGADNRGGIENILEEIAELRREVSYQNDYISSMADPFKRRGYWYFMPPPSSSKVSSHSSQATKDSGVGMKYAASTPIRKPQQNGREDGAPPAASGYWVYSPIRSGLHKSFSNKDADSGGESREESELDDHDDRPFVPPPGYMMYTVFPDGSPVPQGMALYAPPPPLPNNSRPLSPGTVVYGPPPAGAPIVYGPPPPSFSVPLIPVGVLHCNVPEHHALENEVSRLEDIIQHLKSKKQEQRMRVCKRQSEKEIEELHHNIDELLQEKKDLEHEVEELHRTIQKHQQRKDFIDGNVESLMNELKIEKSLKHHEDIVDEIECIEKTLLKRRAELREADRLLAEAESELSRTTEKTKNAVEKFTDAKRNLLQTEKDAEELERRAQETAVSLVKADQQLRLLQADTKDLEQHKTEQEEILKEINKVVAAKDSDFQCLNTKKEKLTEELQKLQKDIETAARTEDHHLQVLRESEALLQAKRAELDKLRSQVTSQQQEMAVLDRQLGHKKEELHLLQGSMVQARADLQEALRLGETEVTEKCNHIRELKGLLEELSFQKGELNVQISEKKNQLSLIKQEIEKEEENLQVVLGQMSKHKTELKNILDMLQLENNELQALKLQHEQKMSELEKAQVEVLEEKLELENLQQTALRQKGEIEWQKQLLERDKREIERITAETRALQLCVESLCKQKEDLEEKCDSWEKKLAQTKRVLAATEENSKAKQSDLEKLELDIRKLEQELDQLNRNKLSLHRDIAAMQQQVQEKREAVNSLQEELADVQAHLNLAKQDLLHTTKRQDVLLGEQTRLQEDLQERRKKLEVCQKEEATKRQHLQALQTEMEANEAELARQETTFQRLQKERESEEKKLEASRATLQEQQQQLEKEIADQKNVLDQVLSQVLGAKERARTLQQEERWGETLEKTLSQTQQQLSEREQQLLEKSAELLALQKEADSMRADFSLLRNQFLTERKKAEKQVASLREALKTQRSQLEKTLLEQKQENSSMQKEMATIEQVAQDNHERARRLMRELSQMQQEYAELKKQMVNQKDLERRQVEISEAMRTLKSEVKDEIRASLKNLNQFLPELPADLEAILERSEHLDGGLESLKENFPFALSEKPSPLEEKLHFSRVHIMDEHWRGEALREKLRHREDRLKAQLRHCMSKQAEVLSKGKRQTEGTLHSLRRQVDALGELVTSTSVESTSSATLSQLESSLAEDSHLGPSQSSFHQVPQGPSQSVDSYRH

Target ORF information:

RefSeq Version	XM_006054146.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centriolin (CNTRL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006054146.1

ORF Insert Sequence:

ATGCTGATAG AGGGCGAGCT GGAAGGCTAT GGGCTTAGGA ATGTTGGCGG CCTGTATTTA 
GATGATGGAT GTGGAACAGG CAGGCCCACT GTGGAAGTGG GGTCAGCAGC GCTTTCTAAT 
GGATTACATA ATCCTCAGGC TTCTGGCTTA AAAGAGGTAG AAAGACTGGA AAGAGACCTG 
GAGAAGAAGA TGATGGAAAC TGAAGAGCTT AAAAGCAAAC AAGCACAGTT CCTTGAAGAA 
ATTAAAAATC AAGATAAGTT GAACAGATCA TTAAAAGAGG AGGCTATGCT ACAAAAACAG 
AGCTGTGAGG AGCTCGAGAG TAACCTTAAC ACGAAAAATG AACTGCTAAG ACAGAAGACC 
ATGGAACTAA CGCGAGCATG TCAAAAACAG TATGAACTGG AACAGGAGTT GGCTTTTTAC 
AAAATCGATG CTAAATTTGA GCCACTGAAT TATTATCCCT CAGAGTATGT TGACGTTGAT 
AAAGCCCCAG ATGAAAGCCC TTACATTGGC AAGTCCAGAT ACAAGAGAAA TATGTTCGCC 
ACAGAGAGTT ACATTGTGAA TAATGCCCAG CCAGTTGAGA TCAAGAGGAT GGAGCCAGAG 
GAAGGGGAGC AACTTAGAAA CGAGCATGTG AGCTTGAAAG CCCATATGCC ACTGGACACG 
GAGCTGGAAG ACAAAGAGAA CAAAATAAGT ACAGCACAAG CTCGACTATC AGCACTGCAT 
GATGAAATAG AAAAGGCAGA ACAACAAATT TTAAGAGCCA CTGAAGAATT TAAACAACTG 
GAAGAAGCTG TACAACTTAA AAAGATTTCA GAAGCAGAGA AAGACTTTCT TCTCAAGCAG 
CTGAGTGGCA GGATACAGCT TCTAAATAAA TTACGCCAGG AAGCTCTTGA TCTAGAAATG 
CAGATGGAAA AGCAAAGAGG AGAAATTGCT GAAAAGCAGG AGGAAATCAA GGACCTCCAA 
AGAGTTATTG ATAGTCTGGA TTCCAAAGAC CCAAAACATT GCCATATGAA GGCTCAGAAA 
AGGGGTAAAG AACAACAATT TGACATTATG AACAAGCAGT ACAAACAACT TGAGAGCCGT 
TTGGACGAGA TACTTTCTAG AATTGCTAAA GAAACTGAAG AGATTAAGGA CCTTGAACAA 
CAGCTTACTG AAGGCCAGAT AGCAGCAAAT GAAGCCCTGA AGAAGGACTT AGAAGGCGTC 
ATCAGTGGCC TGCAAGAATA CCTGGGCACT GTGAAGGGCC AGGCCGCTCA GGCCCAGAGC 
GAGTGCAGAA GGCTGCAGGA TGAGAAAGAG GCGCTGCTGC AGAGGTTGGC CGAGGTCGAG 
CAGGAAAGGG ACCAGCTGGA AGCAGTCGCC TTAGATGCAG AAGCTATGAG GAAGGAGCTT 
GCAGAGCTAG AAAGCGCCCT CCAAGAGCAG CATGAGGTGA ACGCGTCCCT GCAGCAGACC 
CACGGAGACC TCAGTGCCTA CGAGGCTGAG CTAGAGGCAC AGCTGAGGAT GAGGGATGCC 
GAAGCCAGCC AGCTCAAAGA AGAGCTGGGA GAACTAACAA GGCTCAGCCA GCTAGAACAG 
TCAACTCTAC AAACAGAACT TGAGAAAGAA AAACAAGCCC TCAAGAACGC CCTGGGAAAA 
GCCCAGCTCT CGGAAGAAAA AGAACAAGAA AACAGTGAGC TCCGCACCCA GCTTAAACAG 
CTACAGGATG ACAATAACCT GTTAAAACAG CAACTTAAAG ATTTCCAGAA TCACCTTAAT 
CAGGTAGCTG ATGGTCTGAT TCGTCCAGAA GAAGTGGCAG CTCGTGTAGA TGAAGTAAGG 
AGAAAATTGA AATCAGGCGC TGGAGAGATG AGCAGAATCC ACAGTCCTTC AGATGTCTTA 
GGGAAAAGTC TTGCTGACTT GCAGATACAA TTCAGTGAAA TGCTGACACG TTCTCAGTGG 
GAGAAAGAGG AAGCCCAAGG GAGGGAAAGA AAACTCCAAG AAGAAATGGC TCTGCATCAG 
GAGAAGCTGG TGGATGGACA GGAAGCGTTC AGGCAGGCCT GTGAGAGAGC GCTAGAAGCA 
AGGATTAATT TTGACAAGAG GCAACATGAA GCAAGAATTC AGCAGTTAGA GAATGAGATT 
CACTATTTAC AAGAAAATCT AAAAAGTATG GAGGAAATCC AAGGGCTTAC AGATCTGCAG 
CTGCAGGAAG CTGATGAAGA GAAGGAGAGA ATTCTAGCCC AACTCCAGGA GTTAGAGAAG 
AAGAAGAAAC TTGAAGATGC CAAATCTCAG GCACAGGTTC TAGGTTTAGA TAAAGAATTG 
AAGAAACTAA AGAAGGCTGT GGCCACCTCT GATAAGATAG CCACAGCTGA GCTCACCATC 
GCCAAAGACC AGCTCAAGTC CCTTCATGGA ACTGTTATGA AAATTAACCA GGAGCGTGCA 
GAGGACCTGC AGGAAGCAGA GCGGTTCAGC AGAAAGGCAG CACAGGCCGC GAGGGATCTG 
ACCCGAGCGG AAGCGGAGAT CGAACTCCTG CAGAACCTCC TCAGGGAAAG AGAGGAGCAG 
ATGGAGAAGG TAGAGGCAGG CACCCGAGGA GCAAGCTCAC AGGCGCTGGA AATCGAGAGG 
TGGAATGAGA CCATGGAGAG ACAGAGGAGC GAGATCGCAA GGCTACGGGA CTCACTAGAC 
CGCACCGGGG CGGATAACAG AGGAGGCATT GAAAATATTT TGGAAGAAAT AGCTGAACTC 
CGACGTGAAG TTTCTTACCA GAATGATTAC ATCAGCAGCA TGGCAGATCC TTTCAAAAGA 
CGAGGCTATT GGTACTTTAT GCCACCACCA TCATCATCAA AAGTTTCCAG CCACAGCTCC 
CAGGCCACAA AGGACTCTGG TGTCGGGATG AAGTACGCAG CCTCGACTCC TATTAGAAAA 
CCACAGCAGA ACGGGAGGGA AGACGGTGCC CCTCCAGCTG CCTCCGGATA CTGGGTGTAT 
TCCCCCATCA GGAGCGGCTT ACATAAATCG TTTTCAAATA AAGATGCAGA CAGTGGAGGA 
GAGAGCCGGG AGGAGAGCGA GCTGGATGAC CACGATGACC GCCCGTTCGT CCCTCCTCCT 
GGATACATGA TGTACACCGT GTTCCCCGAC GGCTCTCCTG TACCACAGGG CATGGCCCTC 
TACGCACCGC CGCCTCCCTT GCCCAACAAT AGCCGACCTC TCAGCCCTGG CACTGTTGTG 
TATGGCCCGC CCCCTGCTGG TGCCCCCATT GTCTACGGGC CCCCACCGCC CAGCTTCTCC 
GTCCCCCTCA TCCCAGTGGG CGTGCTGCAC TGCAACGTCC CGGAGCACCA TGCCTTAGAG 
AATGAGGTTT CTAGATTGGA AGACATAATA CAGCATTTAA AATCAAAGAA GCAGGAACAG 
AGGATGAGAG TATGCAAGCG GCAGTCTGAG AAAGAAATAG AAGAACTGCA TCATAATATT 
GATGAACTTT TGCAAGAGAA GAAAGACTTA GAGCACGAAG TAGAAGAATT ACATAGAACC 
ATCCAGAAAC ATCAACAGCG AAAAGACTTC ATTGATGGAA ATGTTGAGAG TCTTATGAAT 
GAACTAAAAA TAGAAAAATC ACTCAAGCAC CATGAAGATA TTGTAGATGA AATTGAGTGC 
ATCGAGAAGA CTCTCCTGAA ACGTCGAGCA GAGCTCAGGG AGGCTGACCG ACTGCTGGCG 
GAAGCCGAGA GTGAACTTTC ACGCACAACA GAGAAAACAA AAAATGCTGT TGAAAAGTTC 
ACTGATGCCA AGAGGAATTT GTTGCAAACT GAGAAAGATG CTGAGGAGTT AGAAAGGAGA 
GCTCAGGAAA CAGCTGTTAG CCTTGTCAAG GCTGATCAAC AGCTCAGATT GTTACAGGCG 
GACACAAAGG ATTTGGAGCA GCACAAAACT GAGCAAGAAG AGATCTTGAA AGAGATAAAC 
AAAGTTGTTG CAGCAAAAGA CTCAGACTTC CAGTGTTTAA ACACGAAGAA GGAGAAGCTA 
ACAGAAGAGC TGCAGAAGCT GCAGAAAGAC ATCGAGACGG CAGCGCGCAC GGAGGATCAC 
CACCTGCAGG TCCTGAGAGA GTCGGAGGCC CTCCTGCAGG CCAAGAGAGC GGAGCTGGAC 
AAGCTGAGGA GCCAGGTAAC GAGTCAGCAG CAGGAGATGG CTGTCTTGGA CAGACAGCTG 
GGGCATAAGA AGGAGGAGCT GCATCTACTC CAGGGAAGCA TGGTCCAGGC CAGAGCTGAC 
CTCCAGGAAG CGCTGAGGCT GGGAGAAACT GAAGTAACTG AGAAGTGCAA CCACATCAGG 
GAACTAAAAG GTCTTCTGGA AGAACTGAGT TTTCAGAAAG GAGAACTGAA TGTCCAGATT 
AGTGAGAAAA AAAATCAACT TTCACTTATA AAGCAAGAAA TTGAAAAAGA GGAAGAAAAT 
CTTCAGGTAG TTTTAGGGCA GATGTCTAAA CATAAAACTG AACTTAAGAA TATTCTGGAC 
ATGTTGCAAC TTGAAAACAA TGAGCTGCAA GCTTTGAAGC TACAGCATGA GCAAAAGATG 
TCTGAGTTAG AGAAGGCTCA GGTTGAAGTG CTGGAGGAGA AACTGGAGTT GGAGAATTTG 
CAGCAGACAG CCCTGCGACA GAAAGGGGAG ATCGAGTGGC AGAAGCAGCT CTTAGAGAGG 
GACAAGCGAG AGATTGAGCG GATAACTGCT GAGACCCGAG CATTGCAGTT GTGTGTTGAG 
TCTTTGTGCA AACAAAAGGA AGATCTTGAG GAGAAATGTG ACAGCTGGGA GAAGAAGCTG 
GCACAGACCA AACGGGTTTT AGCAGCCACA GAAGAGAATA GCAAAGCAAA GCAATCAGAC 
TTAGAAAAGT TGGAATTGGA TATCAGAAAA CTGGAACAGG AACTAGACCA GCTAAACAGA 
AACAAACTCT CACTGCACAG AGACATCGCG GCCATGCAGC AGCAGGTCCA AGAAAAACGA 
GAAGCAGTAA ACTCATTACA GGAGGAACTA GCTGATGTCC AAGCCCATTT GAACCTAGCG 
AAACAGGACC TGCTCCACAC CACCAAGCGC CAGGATGTCC TGCTTGGCGA GCAGACCAGG 
CTGCAGGAGG ACCTCCAGGA ACGCAGAAAG AAGTTGGAAG TCTGCCAAAA AGAAGAGGCA 
ACAAAGCGGC AGCACCTGCA AGCGCTTCAG ACGGAGATGG AGGCGAACGA GGCCGAGCTG 
GCCCGGCAGG AGACGACATT TCAGAGACTC CAGAAAGAGA GAGAAAGTGA AGAAAAAAAG 
TTAGAAGCCA GTCGAGCGAC TCTGCAGGAG CAGCAGCAGC AGCTGGAAAA GGAAATCGCA 
GACCAGAAGA ACGTGCTGGA CCAGGTGCTC AGCCAGGTGC TGGGGGCCAA GGAGCGCGCC 
AGGACTCTGC AGCAGGAGGA GAGGTGGGGT GAGACCCTGG AGAAGACGCT CTCCCAGACC 
CAGCAACAGC TCTCAGAACG GGAGCAGCAG TTGTTGGAAA AGTCAGCTGA GCTGCTGGCT 
CTTCAGAAAG AGGCAGACTC GATGAGGGCA GACTTCAGCC TCTTGCGAAA CCAGTTCTTG 
ACGGAGAGGA AGAAAGCCGA GAAGCAGGTA GCCAGCCTCA GGGAAGCGCT CAAGACCCAG 
CGCAGCCAGC TGGAGAAGAC CCTTCTCGAG CAGAAGCAGG AGAACAGCTC CATGCAGAAG 
GAGATGGCGA CTATCGAGCA GGTGGCGCAG GACAACCACG AGCGGGCCCG GCGCCTGATG 
CGGGAGCTCA GCCAGATGCA GCAGGAGTAC GCGGAGCTCA AGAAGCAGAT GGTAAACCAG 
AAAGATCTGG AAAGAAGACA AGTAGAAATC AGTGAAGCGA TGAGGACACT TAAGTCTGAA 
GTGAAAGACG AGATCAGAGC CAGCCTGAAG AACCTCAACC AGTTTCTCCC GGAGCTGCCA 
GCAGACCTGG AGGCCATTCT GGAAAGGAGC GAACACCTAG ACGGAGGGCT GGAGAGCTTG 
AAGGAGAACT TTCCATTCGC CCTGAGTGAG AAGCCATCAC CTTTGGAAGA GAAACTACAT 
TTTTCTCGCG TTCACATAAT GGATGAGCAC TGGCGTGGAG AAGCGCTCCG GGAGAAGCTG 
CGGCACCGTG AGGACCGACT CAAGGCCCAG CTCCGACACT GTATGTCCAA GCAGGCAGAG 
GTGTTAAGCA AAGGGAAGCG GCAGACCGAG GGCACGTTAC ACAGCCTGAG GAGGCAGGTC 
GACGCCTTAG GGGAGCTGGT CACCAGCACG TCTGTGGAGT CCACGTCGTC AGCCACTCTG 
TCTCAGCTCG AATCTTCCCT TGCAGAGGAC TCCCATCTTG GACCAAGTCA GAGCTCCTTC 
CATCAAGTTC CACAAGGTCC ATCACAATCA GTAGACAGTT ACCGACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTRL ( XM_006054147.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006054209.1 Bubalus bubalis centriolin (CNTRL), transcript variant X5, mRNA.

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CloneID	OBu11632
Clone ID Related Accession (Same CDS sequence)	XM_006054147.1
Accession Version	XM_006054147.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	centriolin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784589.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCTAAGACA GAAGACCATG 
GAACTAACGC GAGCATGTCA AAAACAGTAT GAACTGGAAC AGGAGTTGGC TTTTTACAAA 
ATCGATGCTA AATTTGAGCC ACTGAATTAT TATCCCTCAG AGTATGTTGA CGTTGATAAA 
GCCCCAGATG AAAGCCCTTA CATTGGCAAG TCCAGATACA AGAGAAATAT GTTCGCCACA 
GAGAGTTACA TTGTGAATAA TGCCCAGCCA GTTGAGATCA AGAGGATGGA GCCAGAGGAA 
GGGGAGCAAC TTAGAAACGA GCATGTGAGC TTGAAAGCCC ATATGCCACT GGACACGGAG 
CTGGAAGACA AAGAGAACAA AATAAGTACA GCACAAGCTC GACTATCAGC ACTGCATGAT 
GAAATAGAAA AGGCAGAACA ACAAATTTTA AGAGCCACTG AAGAATTTAA ACAACTGGAA 
GAAGCTGTAC AACTTAAAAA GATTTCAGAA GCAGAGAAAG ACTTTCTTCT CAAGCAGCTG 
AGTGGCAGGA TACAGCTTCT AAATAAATTA CGCCAGGAAG CTCTTGATCT AGAAATGCAG 
ATGGAAAAGC AAAGAGGAGA AATTGCTGAA AAGCAGGAGG AAATCAAGGA CCTCCAAAGA 
GTTATTGATA GTCTGGATTC CAAAGACCCA AAACATTGCC ATATGAAGGC TCAGAAAAGG 
GGTAAAGAAC AACAATTTGA CATTATGAAC AAGCAGTACA AACAACTTGA GAGCCGTTTG 
GACGAGATAC TTTCTAGAAT TGCTAAAGAA ACTGAAGAGA TTAAGGACCT TGAACAACAG 
CTTACTGAAG GCCAGATAGC AGCAAATGAA GCCCTGAAGA AGGACTTAGA AGGCGTCATC 
AGTGGCCTGC AAGAATACCT GGGCACTGTG AAGGGCCAGG CCGCTCAGGC CCAGAGCGAG 
TGCAGAAGGC TGCAGGATGA GAAAGAGGCG CTGCTGCAGA GGTTGGCCGA GGTCGAGCAG 
GAAAGGGACC AGCTGGAAGC AGTCGCCTTA GATGCAGAAG CTATGAGGAA GGAGCTTGCA 
GAGCTAGAAA GCGCCCTCCA AGAGCAGCAT GAGGTGAACG CGTCCCTGCA GCAGACCCAC 
GGAGACCTCA GTGCCTACGA GGCTGAGCTA GAGGCACAGC TGAGGATGAG GGATGCCGAA 
GCCAGCCAGC TCAAAGAAGA GCTGGGAGAA CTAACAAGGC TCAGCCAGCT AGAACAGTCA 
ACTCTACAAA CAGAACTTGA GAAAGAAAAA CAAGCCCTCA AGAACGCCCT GGGAAAAGCC 
CAGCTCTCGG AAGAAAAAGA ACAAGAAAAC AGTGAGCTCC GCACCCAGCT TAAACAGCTA 
CAGGATGACA ATAACCTGTT AAAACAGCAA CTTAAAGATT TCCAGAATCA CCTTAATCAG 
GTAGCTGATG GTCTGATTCG TCCAGAAGAA GTGGCAGCTC GTGTAGATGA AGTAAGGAGA 
AAATTGAAAT CAGGCGCTGG AGAGATGAGC AGAATCCACA GTCCTTCAGA TGTCTTAGGG 
AAAAGTCTTG CTGACTTGCA GATACAATTC AGTGAAATGC TGACACGTTC TCAGTGGGAG 
AAAGAGGAAG CCCAAGGGAG GGAAAGAAAA CTCCAAGAAG AAATGGCTCT GCATCAGGAG 
AAGCTGGTGG ATGGACAGGA AGCGTTCAGG CAGGCCTGTG AGAGAGCGCT AGAAGCAAGG 
ATTAATTTTG ACAAGAGGCA ACATGAAGCA AGAATTCAGC AGTTAGAGAA TGAGATTCAC 
TATTTACAAG AAAATCTAAA AAGTATGGAG GAAATCCAAG GGCTTACAGA TCTGCAGCTG 
CAGGAAGCTG ATGAAGAGAA GGAGAGAATT CTAGCCCAAC TCCAGGAGTT AGAGAAGAAG 
AAGAAACTTG AAGATGCCAA ATCTCAGGCA CAGGTTCTAG GTTTAGATAA AGAATTGAAG 
AAACTAAAGA AGGCTGTGGC CACCTCTGAT AAGATAGCCA CAGCTGAGCT CACCATCGCC 
AAAGACCAGC TCAAGTCCCT TCATGGAACT GTTATGAAAA TTAACCAGGA GCGTGCAGAG 
GACCTGCAGG AAGCAGAGCG GTTCAGCAGA AAGGCAGCAC AGGCCGCGAG GGATCTGACC 
CGAGCGGAAG CGGAGATCGA ACTCCTGCAG AACCTCCTCA GGGAAAGAGA GGAGCAGATG 
GAGAAGGTAG AGGCAGGCAC CCGAGGAGCA AGCTCACAGG CGCTGGAAAT CGAGAGGTGG 
AATGAGACCA TGGAGAGACA GAGGAGCGAG ATCGCAAGGC TACGGGACTC ACTAGACCGC 
ACCGGGGCGG ATAACAGAGG AGGCATTGAA AATATTTTGG AAGAAATAGC TGAACTCCGA 
CGTGAAGTTT CTTACCAGAA TGATTACATC AGCAGCATGG CAGATCCTTT CAAAAGACGA 
GGCTATTGGT ACTTTATGCC ACCACCATCA TCATCAAAAG TTTCCAGCCA CAGCTCCCAG 
GCCACAAAGG ACTCTGGTGT CGGGATGAAG TACGCAGCCT CGACTCCTAT TAGAAAACCA 
CAGCAGAACG GGAGGGAAGA CGGTGCCCCT CCAGCTGCCT CCGGATACTG GGTGTATTCC 
CCCATCAGGA GCGGCTTACA TAAATCGTTT TCAAATAAAG ATGCAGACAG TGGAGGAGAG 
AGCCGGGAGG AGAGCGAGCT GGATGACCAC GATGACCGCC CGTTCGTCCC TCCTCCTGGA 
TACATGATGT ACACCGTGTT CCCCGACGGC TCTCCTGTAC CACAGGGCAT GGCCCTCTAC 
GCACCGCCGC CTCCCTTGCC CAACAATAGC CGACCTCTCA GCCCTGGCAC TGTTGTGTAT 
GGCCCGCCCC CTGCTGGTGC CCCCATTGTC TACGGGCCCC CACCGCCCAG CTTCTCCGTC 
CCCCTCATCC CAGTGGGCGT GCTGCACTGC AACGTCCCGG AGCACCATGC CTTAGAGAAT 
GAGGTTTCTA GATTGGAAGA CATAATACAG CATTTAAAAT CAAAGAAGCA GGAACAGAGG 
ATGAGAGTAT GCAAGCGGCA GTCTGAGAAA GAAATAGAAG AACTGCATCA TAATATTGAT 
GAACTTTTGC AAGAGAAGAA AGACTTAGAG CACGAAGTAG AAGAATTACA TAGAACCATC 
CAGAAACATC AACAGCGAAA AGACTTCATT GATGGAAATG TTGAGAGTCT TATGAATGAA 
CTAAAAATAG AAAAATCACT CAAGCACCAT GAAGATATTG TAGATGAAAT TGAGTGCATC 
GAGAAGACTC TCCTGAAACG TCGAGCAGAG CTCAGGGAGG CTGACCGACT GCTGGCGGAA 
GCCGAGAGTG AACTTTCACG CACAACAGAG AAAACAAAAA ATGCTGTTGA AAAGTTCACT 
GATGCCAAGA GGAATTTGTT GCAAACTGAG AAAGATGCTG AGGAGTTAGA AAGGAGAGCT 
CAGGAAACAG CTGTTAGCCT TGTCAAGGCT GATCAACAGC TCAGATTGTT ACAGGCGGAC 
ACAAAGGATT TGGAGCAGCA CAAAACTGAG CAAGAAGAGA TCTTGAAAGA GATAAACAAA 
GTTGTTGCAG CAAAAGACTC AGACTTCCAG TGTTTAAACA CGAAGAAGGA GAAGCTAACA 
GAAGAGCTGC AGAAGCTGCA GAAAGACATC GAGACGGCAG CGCGCACGGA GGATCACCAC 
CTGCAGGTCC TGAGAGAGTC GGAGGCCCTC CTGCAGGCCA AGAGAGCGGA GCTGGACAAG 
CTGAGGAGCC AGGTAACGAG TCAGCAGCAG GAGATGGCTG TCTTGGACAG ACAGCTGGGG 
CATAAGAAGG AGGAGCTGCA TCTACTCCAG GGAAGCATGG TCCAGGCCAG AGCTGACCTC 
CAGGAAGCGC TGAGGCTGGG AGAAACTGAA GTAACTGAGA AGTGCAACCA CATCAGGGAA 
CTAAAAGGTC TTCTGGAAGA ACTGAGTTTT CAGAAAGGAG AACTGAATGT CCAGATTAGT 
GAGAAAAAAA ATCAACTTTC ACTTATAAAG CAAGAAATTG AAAAAGAGGA AGAAAATCTT 
CAGGTAGTTT TAGGGCAGAT GTCTAAACAT AAAACTGAAC TTAAGAATAT TCTGGACATG 
TTGCAACTTG AAAACAATGA GCTGCAAGCT TTGAAGCTAC AGCATGAGCA AAAGATGTCT 
GAGTTAGAGA AGGCTCAGGT TGAAGTGCTG GAGGAGAAAC TGGAGTTGGA GAATTTGCAG 
CAGACAGCCC TGCGACAGAA AGGGGAGATC GAGTGGCAGA AGCAGCTCTT AGAGAGGGAC 
AAGCGAGAGA TTGAGCGGAT AACTGCTGAG ACCCGAGCAT TGCAGTTGTG TGTTGAGTCT 
TTGTGCAAAC AAAAGGAAGA TCTTGAGGAG AAATGTGACA GCTGGGAGAA GAAGCTGGCA 
CAGACCAAAC GGGTTTTAGC AGCCACAGAA GAGAATAGCA AAGCAAAGCA ATCAGACTTA 
GAAAAGTTGG AATTGGATAT CAGAAAACTG GAACAGGAAC TAGACCAGCT AAACAGAAAC 
AAACTCTCAC TGCACAGAGA CATCGCGGCC ATGCAGCAGC AGGTCCAAGA AAAACGAGAA 
GCAGTAAACT CATTACAGGA GGAACTAGCT GATGTCCAAG CCCATTTGAA CCTAGCGAAA 
CAGGACCTGC TCCACACCAC CAAGCGCCAG GATGTCCTGC TTGGCGAGCA GACCAGGCTG 
CAGGAGGACC TCCAGGAACG CAGAAAGAAG TTGGAAGTCT GCCAAAAAGA AGAGGCAACA 
AAGCGGCAGC ACCTGCAAGC GCTTCAGACG GAGATGGAGG CGAACGAGGC CGAGCTGGCC 
CGGCAGGAGA CGGTACCGCA CCCGCGGGCA CGCGCGGGGG GGCACTGCCA CGCGCAGAGC 
GCTCTTCCCC TGGGGTCTGA GGTGGAGGCG AACGAGAGCG AGCTGGCCCG GCAGGAGACG 
GTACCGCACC TGCGTGGCAA GCAAGGGGGG CACTGCCACG CGGGGAGCGC TCTTCCCCTG 
GCGCGGCGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006054209.1
CDS	129..6143
Translation	MKKGPQQKISKTKMPPSSHSPSPSSLTSNMRSRSLSPLNGSETLPFHSGRQWYGQVEIIGENSMLLDYHDQKEADSHAGVRYITEALVKKLTKQENLTLVKSLNLSLSKDGGKKFKYIENLEKCTKLEILNLSHNLIGKIEKVDKLLKLRELNLSYNKICKIEGIENMHNLQKLNLAGNEIEHIPAWLGKKLKCLRVLNLKANKISSLQDVSKLKPLQDLTSLILAENPVVTLPHYLQFTIFHLRSLESLEGQPVTTQDRQEAFERFSLEEVERLERDLEKKMMETEELKSKQAQFLEEIKNQDKLNRSLKEEAMLQKQSCEELESNLNTKNELLRQKTMELTRACQKQYELEQELAFYKIDAKFEPLNYYPSEYVDVDKAPDESPYIGKSRYKRNMFATESYIVNNAQPVEIKRMEPEEGEQLRNEHVSLKAHMPLDTELEDKENKISTAQARLSALHDEIEKAEQQILRATEEFKQLEEAVQLKKISEAEKDFLLKQLSGRIQLLNKLRQEALDLEMQMEKQRGEIAEKQEEIKDLQRVIDSLDSKDPKHCHMKAQKRGKEQQFDIMNKQYKQLESRLDEILSRIAKETEEIKDLEQQLTEGQIAANEALKKDLEGVISGLQEYLGTVKGQAAQAQSECRRLQDEKEALLQRLAEVEQERDQLEAVALDAEAMRKELAELESALQEQHEVNASLQQTHGDLSAYEAELEAQLRMRDAEASQLKEELGELTRLSQLEQSTLQTELEKEKQALKNALGKAQLSEEKEQENSELRTQLKQLQDDNNLLKQQLKDFQNHLNQVADGLIRPEEVAARVDEVRRKLKSGAGEMSRIHSPSDVLGKSLADLQIQFSEMLTRSQWEKEEAQGRERKLQEEMALHQEKLVDGQEAFRQACERALEARINFDKRQHEARIQQLENEIHYLQENLKSMEEIQGLTDLQLQEADEEKERILAQLQELEKKKKLEDAKSQAQVLGLDKELKKLKKAVATSDKIATAELTIAKDQLKSLHGTVMKINQERAEDLQEAERFSRKAAQAARDLTRAEAEIELLQNLLREREEQMEKVEAGTRGASSQALEIERWNETMERQRSEIARLRDSLDRTGADNRGGIENILEEIAELRREVSYQNDYISSMADPFKRRGYWYFMPPPSSSKVSSHSSQATKDSGVGMKYAASTPIRKPQQNGREDGAPPAASGYWVYSPIRSGLHKSFSNKDADSGGESREESELDDHDDRPFVPPPGYMMYTVFPDGSPVPQGMALYAPPPPLPNNSRPLSPGTVVYGPPPAGAPIVYGPPPPSFSVPLIPVGVLHCNVPEHHALENEVSRLEDIIQHLKSKKQEQRMRVCKRQSEKEIEELHHNIDELLQEKKDLEHEVEELHRTIQKHQQRKDFIDGNVESLMNELKIEKSLKHHEDIVDEIECIEKTLLKRRAELREADRLLAEAESELSRTTEKTKNAVEKFTDAKRNLLQTEKDAEELERRAQETAVSLVKADQQLRLLQADTKDLEQHKTEQEEILKEINKVVAAKDSDFQCLNTKKEKLTEELQKLQKDIETAARTEDHHLQVLRESEALLQAKRAELDKLRSQVTSQQQEMAVLDRQLGHKKEELHLLQGSMVQARADLQEALRLGETEVTEKCNHIRELKGLLEELSFQKGELNVQISEKKNQLSLIKQEIEKEEENLQVVLGQMSKHKTELKNILDMLQLENNELQALKLQHEQKMSELEKAQVEVLEEKLELENLQQTALRQKGEIEWQKQLLERDKREIERITAETRALQLCVESLCKQKEDLEEKCDSWEKKLAQTKRVLAATEENSKAKQSDLEKLELDIRKLEQELDQLNRNKLSLHRDIAAMQQQVQEKREAVNSLQEELADVQAHLNLAKQDLLHTTKRQDVLLGEQTRLQEDLQERRKKLEVCQKEEATKRQHLQALQTEMEANEAELARQETVPHPRARAGGHCHAQSALPLGSEVEANESELARQETVPHLRGKQGGHCHAGSALPLARRC

Target ORF information:

RefSeq Version	XM_006054147.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centriolin (CNTRL), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006054147.1

ORF Insert Sequence:

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCTAAGACA GAAGACCATG 
GAACTAACGC GAGCATGTCA AAAACAGTAT GAACTGGAAC AGGAGTTGGC TTTTTACAAA 
ATCGATGCTA AATTTGAGCC ACTGAATTAT TATCCCTCAG AGTATGTTGA CGTTGATAAA 
GCCCCAGATG AAAGCCCTTA CATTGGCAAG TCCAGATACA AGAGAAATAT GTTCGCCACA 
GAGAGTTACA TTGTGAATAA TGCCCAGCCA GTTGAGATCA AGAGGATGGA GCCAGAGGAA 
GGGGAGCAAC TTAGAAACGA GCATGTGAGC TTGAAAGCCC ATATGCCACT GGACACGGAG 
CTGGAAGACA AAGAGAACAA AATAAGTACA GCACAAGCTC GACTATCAGC ACTGCATGAT 
GAAATAGAAA AGGCAGAACA ACAAATTTTA AGAGCCACTG AAGAATTTAA ACAACTGGAA 
GAAGCTGTAC AACTTAAAAA GATTTCAGAA GCAGAGAAAG ACTTTCTTCT CAAGCAGCTG 
AGTGGCAGGA TACAGCTTCT AAATAAATTA CGCCAGGAAG CTCTTGATCT AGAAATGCAG 
ATGGAAAAGC AAAGAGGAGA AATTGCTGAA AAGCAGGAGG AAATCAAGGA CCTCCAAAGA 
GTTATTGATA GTCTGGATTC CAAAGACCCA AAACATTGCC ATATGAAGGC TCAGAAAAGG 
GGTAAAGAAC AACAATTTGA CATTATGAAC AAGCAGTACA AACAACTTGA GAGCCGTTTG 
GACGAGATAC TTTCTAGAAT TGCTAAAGAA ACTGAAGAGA TTAAGGACCT TGAACAACAG 
CTTACTGAAG GCCAGATAGC AGCAAATGAA GCCCTGAAGA AGGACTTAGA AGGCGTCATC 
AGTGGCCTGC AAGAATACCT GGGCACTGTG AAGGGCCAGG CCGCTCAGGC CCAGAGCGAG 
TGCAGAAGGC TGCAGGATGA GAAAGAGGCG CTGCTGCAGA GGTTGGCCGA GGTCGAGCAG 
GAAAGGGACC AGCTGGAAGC AGTCGCCTTA GATGCAGAAG CTATGAGGAA GGAGCTTGCA 
GAGCTAGAAA GCGCCCTCCA AGAGCAGCAT GAGGTGAACG CGTCCCTGCA GCAGACCCAC 
GGAGACCTCA GTGCCTACGA GGCTGAGCTA GAGGCACAGC TGAGGATGAG GGATGCCGAA 
GCCAGCCAGC TCAAAGAAGA GCTGGGAGAA CTAACAAGGC TCAGCCAGCT AGAACAGTCA 
ACTCTACAAA CAGAACTTGA GAAAGAAAAA CAAGCCCTCA AGAACGCCCT GGGAAAAGCC 
CAGCTCTCGG AAGAAAAAGA ACAAGAAAAC AGTGAGCTCC GCACCCAGCT TAAACAGCTA 
CAGGATGACA ATAACCTGTT AAAACAGCAA CTTAAAGATT TCCAGAATCA CCTTAATCAG 
GTAGCTGATG GTCTGATTCG TCCAGAAGAA GTGGCAGCTC GTGTAGATGA AGTAAGGAGA 
AAATTGAAAT CAGGCGCTGG AGAGATGAGC AGAATCCACA GTCCTTCAGA TGTCTTAGGG 
AAAAGTCTTG CTGACTTGCA GATACAATTC AGTGAAATGC TGACACGTTC TCAGTGGGAG 
AAAGAGGAAG CCCAAGGGAG GGAAAGAAAA CTCCAAGAAG AAATGGCTCT GCATCAGGAG 
AAGCTGGTGG ATGGACAGGA AGCGTTCAGG CAGGCCTGTG AGAGAGCGCT AGAAGCAAGG 
ATTAATTTTG ACAAGAGGCA ACATGAAGCA AGAATTCAGC AGTTAGAGAA TGAGATTCAC 
TATTTACAAG AAAATCTAAA AAGTATGGAG GAAATCCAAG GGCTTACAGA TCTGCAGCTG 
CAGGAAGCTG ATGAAGAGAA GGAGAGAATT CTAGCCCAAC TCCAGGAGTT AGAGAAGAAG 
AAGAAACTTG AAGATGCCAA ATCTCAGGCA CAGGTTCTAG GTTTAGATAA AGAATTGAAG 
AAACTAAAGA AGGCTGTGGC CACCTCTGAT AAGATAGCCA CAGCTGAGCT CACCATCGCC 
AAAGACCAGC TCAAGTCCCT TCATGGAACT GTTATGAAAA TTAACCAGGA GCGTGCAGAG 
GACCTGCAGG AAGCAGAGCG GTTCAGCAGA AAGGCAGCAC AGGCCGCGAG GGATCTGACC 
CGAGCGGAAG CGGAGATCGA ACTCCTGCAG AACCTCCTCA GGGAAAGAGA GGAGCAGATG 
GAGAAGGTAG AGGCAGGCAC CCGAGGAGCA AGCTCACAGG CGCTGGAAAT CGAGAGGTGG 
AATGAGACCA TGGAGAGACA GAGGAGCGAG ATCGCAAGGC TACGGGACTC ACTAGACCGC 
ACCGGGGCGG ATAACAGAGG AGGCATTGAA AATATTTTGG AAGAAATAGC TGAACTCCGA 
CGTGAAGTTT CTTACCAGAA TGATTACATC AGCAGCATGG CAGATCCTTT CAAAAGACGA 
GGCTATTGGT ACTTTATGCC ACCACCATCA TCATCAAAAG TTTCCAGCCA CAGCTCCCAG 
GCCACAAAGG ACTCTGGTGT CGGGATGAAG TACGCAGCCT CGACTCCTAT TAGAAAACCA 
CAGCAGAACG GGAGGGAAGA CGGTGCCCCT CCAGCTGCCT CCGGATACTG GGTGTATTCC 
CCCATCAGGA GCGGCTTACA TAAATCGTTT TCAAATAAAG ATGCAGACAG TGGAGGAGAG 
AGCCGGGAGG AGAGCGAGCT GGATGACCAC GATGACCGCC CGTTCGTCCC TCCTCCTGGA 
TACATGATGT ACACCGTGTT CCCCGACGGC TCTCCTGTAC CACAGGGCAT GGCCCTCTAC 
GCACCGCCGC CTCCCTTGCC CAACAATAGC CGACCTCTCA GCCCTGGCAC TGTTGTGTAT 
GGCCCGCCCC CTGCTGGTGC CCCCATTGTC TACGGGCCCC CACCGCCCAG CTTCTCCGTC 
CCCCTCATCC CAGTGGGCGT GCTGCACTGC AACGTCCCGG AGCACCATGC CTTAGAGAAT 
GAGGTTTCTA GATTGGAAGA CATAATACAG CATTTAAAAT CAAAGAAGCA GGAACAGAGG 
ATGAGAGTAT GCAAGCGGCA GTCTGAGAAA GAAATAGAAG AACTGCATCA TAATATTGAT 
GAACTTTTGC AAGAGAAGAA AGACTTAGAG CACGAAGTAG AAGAATTACA TAGAACCATC 
CAGAAACATC AACAGCGAAA AGACTTCATT GATGGAAATG TTGAGAGTCT TATGAATGAA 
CTAAAAATAG AAAAATCACT CAAGCACCAT GAAGATATTG TAGATGAAAT TGAGTGCATC 
GAGAAGACTC TCCTGAAACG TCGAGCAGAG CTCAGGGAGG CTGACCGACT GCTGGCGGAA 
GCCGAGAGTG AACTTTCACG CACAACAGAG AAAACAAAAA ATGCTGTTGA AAAGTTCACT 
GATGCCAAGA GGAATTTGTT GCAAACTGAG AAAGATGCTG AGGAGTTAGA AAGGAGAGCT 
CAGGAAACAG CTGTTAGCCT TGTCAAGGCT GATCAACAGC TCAGATTGTT ACAGGCGGAC 
ACAAAGGATT TGGAGCAGCA CAAAACTGAG CAAGAAGAGA TCTTGAAAGA GATAAACAAA 
GTTGTTGCAG CAAAAGACTC AGACTTCCAG TGTTTAAACA CGAAGAAGGA GAAGCTAACA 
GAAGAGCTGC AGAAGCTGCA GAAAGACATC GAGACGGCAG CGCGCACGGA GGATCACCAC 
CTGCAGGTCC TGAGAGAGTC GGAGGCCCTC CTGCAGGCCA AGAGAGCGGA GCTGGACAAG 
CTGAGGAGCC AGGTAACGAG TCAGCAGCAG GAGATGGCTG TCTTGGACAG ACAGCTGGGG 
CATAAGAAGG AGGAGCTGCA TCTACTCCAG GGAAGCATGG TCCAGGCCAG AGCTGACCTC 
CAGGAAGCGC TGAGGCTGGG AGAAACTGAA GTAACTGAGA AGTGCAACCA CATCAGGGAA 
CTAAAAGGTC TTCTGGAAGA ACTGAGTTTT CAGAAAGGAG AACTGAATGT CCAGATTAGT 
GAGAAAAAAA ATCAACTTTC ACTTATAAAG CAAGAAATTG AAAAAGAGGA AGAAAATCTT 
CAGGTAGTTT TAGGGCAGAT GTCTAAACAT AAAACTGAAC TTAAGAATAT TCTGGACATG 
TTGCAACTTG AAAACAATGA GCTGCAAGCT TTGAAGCTAC AGCATGAGCA AAAGATGTCT 
GAGTTAGAGA AGGCTCAGGT TGAAGTGCTG GAGGAGAAAC TGGAGTTGGA GAATTTGCAG 
CAGACAGCCC TGCGACAGAA AGGGGAGATC GAGTGGCAGA AGCAGCTCTT AGAGAGGGAC 
AAGCGAGAGA TTGAGCGGAT AACTGCTGAG ACCCGAGCAT TGCAGTTGTG TGTTGAGTCT 
TTGTGCAAAC AAAAGGAAGA TCTTGAGGAG AAATGTGACA GCTGGGAGAA GAAGCTGGCA 
CAGACCAAAC GGGTTTTAGC AGCCACAGAA GAGAATAGCA AAGCAAAGCA ATCAGACTTA 
GAAAAGTTGG AATTGGATAT CAGAAAACTG GAACAGGAAC TAGACCAGCT AAACAGAAAC 
AAACTCTCAC TGCACAGAGA CATCGCGGCC ATGCAGCAGC AGGTCCAAGA AAAACGAGAA 
GCAGTAAACT CATTACAGGA GGAACTAGCT GATGTCCAAG CCCATTTGAA CCTAGCGAAA 
CAGGACCTGC TCCACACCAC CAAGCGCCAG GATGTCCTGC TTGGCGAGCA GACCAGGCTG 
CAGGAGGACC TCCAGGAACG CAGAAAGAAG TTGGAAGTCT GCCAAAAAGA AGAGGCAACA 
AAGCGGCAGC ACCTGCAAGC GCTTCAGACG GAGATGGAGG CGAACGAGGC CGAGCTGGCC 
CGGCAGGAGA CGGTACCGCA CCCGCGGGCA CGCGCGGGGG GGCACTGCCA CGCGCAGAGC 
GCTCTTCCCC TGGGGTCTGA GGTGGAGGCG AACGAGAGCG AGCTGGCCCG GCAGGAGACG 
GTACCGCACC TGCGTGGCAA GCAAGGGGGG CACTGCCACG CGGGGAGCGC TCTTCCCCTG 
GCGCGGCGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTRL ( XM_025282345.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025138130.1 Bubalus bubalis centriolin (CNTRL), transcript variant X2, mRNA.

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CloneID	OBu63433
Clone ID Related Accession (Same CDS sequence)	XM_025282345.1 , XM_025282344.1
Accession Version	XM_025282345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	centriolin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCCCTTGTC TCGGTTTCTT 
GCGGACCCTT GTCTGCCGAT TCTTTATTCT TCCTTAAAGC TAAGACAGAA GACCATGGAA 
CTAACGCGAG CATGTCAAAA ACAGTATGAA CTGGAACAGG AGTTGGCTTT TTACAAAATC 
GATGCTAAAT TTGAGCCACT GAATTATTAT CCCTCAGAGT ATGTTGACGT TGATAAAGCC 
CCAGATGAAA GCCCTTACAT TGGCAAGTCC AGATACAAGA GAAATATGTT CGCCACAGAG 
AGTTACATTG TGAATAATGC CCAGCCAGTT GAGATCAAGA GGATGGAGCC AGAGGAAGGG 
GAGCAACTTA GAAACGAGCA TGTGAGCTTG AAAGCCCATA TGCCACTGGA CACGGAGCTG 
GAAGACAAAG AGAACAAAAT AAGTACAGCA CAAGCTCGAC TATCAGCACT GCATGATGAA 
ATAGAAAAGG CAGAACAACA AATTTTAAGA GCCACTGAAG AATTTAAACA ACTGGAAGAA 
GCTGTACAAC TTAAAAAGAT TTCAGAAGCA GAGAAAGACT TTCTTCTCAA GCAGCTGAGT 
GGCAGGATAC AGCTTCTAAA TAAATTACGC CAGGAAGCTC TTGATCTAGA AATGCAGATG 
GAAAAGCAAA GAGGAGAAAT TGCTGAAAAG CAGGAGGAAA TCAAGGACCT CCAAAGAGTT 
ATTGATAGTC TGGATTCCAA AGACCCAAAA CATTGCCATA TGAAGGCTCA GAAAAGGGGT 
AAAGAACAAC AATTTGACAT TATGAACAAG CAGTACAAAC AACTTGAGAG CCGTTTGGAC 
GAGATACTTT CTAGAATTGC TAAAGAAACT GAAGAGATTA AGGACCTTGA ACAACAGCTT 
ACTGAAGGCC AGATAGCAGC AAATGAAGCC CTGAAGAAGG ACTTAGAAGG CGTCATCAGT 
GGCCTGCAAG AATACCTGGG CACTGTGAAG GGCCAGGCCG CTCAGGCCCA GAGCGAGTGC 
AGAAGGCTGC AGGATGAGAA AGAGGCGCTG CTGCAGAGGT TGGCCGAGGT CGAGCAGGAA 
AGGGACCAGC TGGAAGCAGT CGCCTTAGAT GCAGAAGCTA TGAGGAAGGA GCTTGCAGAG 
CTAGAAAGCG CCCTCCAAGA GCAGCATGAG GTGAACGCGT CCCTGCAGCA GACCCACGGA 
GACCTCAGTG CCTACGAGGC TGAGCTAGAG GCACAGCTGA GGATGAGGGA TGCCGAAGCC 
AGCCAGCTCA AAGAAGAGCT GGGAGAACTA ACAAGGCTCA GCCAGCTAGA ACAGTCAACT 
CTACAAACAG AACTTGAGAA AGAAAAACAA GCCCTCAAGA ACGCCCTGGG AAAAGCCCAG 
CTCTCGGAAG AAAAAGAACA AGAAAACAGT GAGCTCCGCA CCCAGCTTAA ACAGCTACAG 
GATGACAATA ACCTGTTAAA ACAGCAACTT AAAGATTTCC AGAATCACCT TAATCAGGTA 
GCTGATGGTC TGATTCGTCC AGAAGAAGTG GCAGCTCGTG TAGATGAAGT AAGGAGAAAA 
TTGAAATCAG GCGCTGGAGA GATGAGCAGA ATCCACAGTC CTTCAGATGT CTTAGGGAAA 
AGTCTTGCTG ACTTGCAGAT ACAATTCAGT GAAATGCTGA CACGTTCTCA GTGGGAGAAA 
GAGGAAGCCC AAGGGAGGGA AAGAAAACTC CAAGAAGAAA TGGCTCTGCA TCAGGAGAAG 
CTGGTGGATG GACAGGAAGC GTTCAGGCAG GCCTGTGAGA GAGCGCTAGA AGCAAGGATT 
AATTTTGACA AGAGGCAACA TGAAGCAAGA ATTCAGCAGT TAGAGAATGA GATTCACTAT 
TTACAAGAAA ATCTAAAAAG TATGGAGGAA ATCCAAGGGC TTACAGATCT GCAGCTGCAG 
GAAGCTGATG AAGAGAAGGA GAGAATTCTA GCCCAACTCC AGGAGTTAGA GAAGAAGAAG 
AAACTTGAAG ATGCCAAATC TCAGGCACAG GTTCTAGGTT TAGATAAAGA ATTGAAGAAA 
CTAAAGAAGG CTGTGGCCAC CTCTGATAAG ATAGCCACAG CTGAGCTCAC CATCGCCAAA 
GACCAGCTCA AGTCCCTTCA TGGAACTGTT ATGAAAATTA ACCAGGAGCG TGCAGAGGAC 
CTGCAGGAAG CAGAGCGGTT CAGCAGAAAG GCAGCACAGG CCGCGAGGGA TCTGACCCGA 
GCGGAAGCGG AGATCGAACT CCTGCAGAAC CTCCTCAGGG AGAGAGAGGA GCAGATGGAG 
AAGGTAGAGG CAGGCACCCG AGGAGCAAGC TCACAGGCGC TGGAAATCGA GAGGTGGAAT 
GAGACCATGG AGAGACAGAG GAGCGAGATC GCAAGGCTAC GGGACTCACT AGACCGCACC 
GGGGCGGATA ACAGAGGAGG CATTGAAAAT ATTTTGGAAG AAATAGCTGA ACTCCGACGT 
GAAGTTTCTT ACCAGAATGA TTACATCAGC AGCATGGCAG ATCCTTTCAA AAGACGAGGC 
TATTGGTACT TTATGCCACC ACCATCATCA TCAAAAGTTT CCAGCCACAG CTCCCAGGCC 
ACAAAGGACT CTGGTGTCGG GATGAAGTAC GCAGCCTCGA CTCCTATTAG AAAACCACAG 
CAGAACGGGA GGGAAGACGG TGCCCCTCCA GCTGCCTCCG GATACTGGGT GTATTCCCCC 
ATCAGGAGCG GCTTACATAA ATCGTTTTCA AATAAAGATG CAGACAGTGG AGGAGAGAGC 
CGGGAGGAGA GCGAGCTGGA TGACCACGAT GACCGCCCGT TCGTCCCTCC TCCTGGATAC 
ATGATGTACA CCGTGTTCCC CGACGGCTCT CCTGTACCAC AGGGCATGGC CCTCTACGCA 
CCGCCGCCTC CCTTGCCCAA CAATAGCCGA CCTCTCAGCC CTGGCACTGT TGTGTATGGC 
CCGCCCCCTG CTGGTGCCCC CATTGTCTAC GGGCCCCCAC CGCCCAGCTT CTCCGTCCCC 
CTCATCCCAG TGGGCGTGCT GCACTGCAAC GTCCCGGAGC ACCATGCCTT AGAGAATGAG 
GTTTCTAGAT TGGAAGACAT AATACAGCAT TTAAAATCAA AGAAGCAGGA ACAGAGGATG 
AGAGTATGCA AGCGGCAGTC TGAGAAAGAA ATAGAAGAAC TGCATCATAA TATTGATGAA 
CTTTTGCAAG AGAAGAAAGA CTTAGAGCAC GAAGTAGAAG AATTACATAG AACCATCCAG 
AAACATCAAC AGCAAAAAGA CTTCATTGAT GGAAATGTTG AGAGTCTTAT GAATGAACTA 
AAAATAGAAA AATCACTCAA GCACCATGAA GATATTGTAG ATGAAATTGA GTGCATCGAG 
AAGACTCTCC TGAAACGTCG AGCAGAGCTC AGGGAGGCTG ACCGACTGCT GGCGGAAGCC 
GAGAGTGAAC TTTCACGCAC AACAGAGAAA ACAAAAAATG CTGTTGAAAA GTTCACTGAT 
GCCAAGAGGA ATTTGTTGCA AACTGAGAAA GATGCTGAGG AGTTAGAAAG GAGAGCTCAG 
GAAACAGCTG TTAGCCTTGT CAAGGCTGAT CAACAGCTCA GATTGTTACA GGCGGACACA 
AAGGATTTGG AGCAGCACAA AACTGAGCAA GAAGAGATCT TGAAAGAGAT AAACAAAGTT 
GTTGCAGCAA AAGACTCAGA CTTCCAGTGT TTAAACACGA AGAAGGAGAA GCTAACAGAA 
GAGCTGCAGA AGCTGCAGAA AGACATCGAG ACGGCAGCGC GCACGGAGGA TCACCACCTG 
CAGGTCCTGA GAGAGTCGGA GGCCCTCCTG CAGGCCAAGA GAGCGGAGCT GGACAAGCTG 
AGGAGCCAGG TAACGAGTCA GCAGCAGGAG ATGGCTGTCT TGGACAGACA GCTGGGGCAT 
AAGAAGGAGG AGCTGCATCT ACTCCAGGGA AGCATGGTCC AGGCCAGAGC TGACCTCCAG 
GAAGCGCTGA GGCTGGGAGA AACTGAAGTA ACTGAGAAGT GCAACCACAT CAGGGAACTA 
AAAGGTCTTC TGGAAGAACT GAGTTTTCAG AAAGGAGAAC TGAATGTCCA GATTAGTGAG 
AAAAAAAATC AACTTTCACT TATAAAGCAA GAAATTGAAA AAGAGGAAGA AAATCTTCAG 
GTAGTTTTAG GGCAGATGTC TAAACATAAA ACTGAACTTA AGAATATTCT GGACATGTTG 
CAACTTGAAA ACAATGAGCT GCAAGCTTTG AAGCTACAGC ATGAGCAAAA GATGTCTGAG 
TTAGAGAAGG CTCAGGTTGA AGTGCTGGAG GAGAAACTGG AGTTGGAGAA TTTGCAGCAG 
ACAGCCCTGC GACAGAAAGG GGAGATCGAG TGGCAGAAGC AGCTCTTAGA GAGGGACAAG 
CGAGAGATTG AGCGGATAAC TGCTGAGACC CGAGCATTGC AGTTGTGTGT TGAGTCTTTG 
TGCAAACAAA AGGAAGATCT TGAGGAGAAA TGTGACAGCT GGGAGAAGAA GCTGGCACAG 
ACCAAACGGG TTTTAGCAGC CACAGAAGAG AATAGCAAAG CAAAGCAATC AGACTTAGAA 
AAGTTGGAAT TGGATATCAG AAAACTGGAA CAGGAACTAG ACCAGCTAAA CAGAAACAAA 
CTCTCACTGC ACAGAGACAT CGCGGCCATG CAGCAGCAGG TCCAAGAAAA ACGAGAAGCA 
GTAAACTCAT TACAGGAGGA ACTAGCTGAT GTCCAAGCCC ATTTGAACCT AGCGAAACAG 
GACCTGCTCC ACACCACCAA GCGCCAGGAT GTCCTGCTTG GCGAGCAGAC CAGGCTGCAG 
GAGGACCTCC AGGAACGCAG AAAGAAGTTG GAAGTCTGCC AAAAAGAAGA GGCAACAAAG 
CGGCAGCACC TGCAAGCGCT TCAGACGGAG ATGGAGGCGA ACGAGGCCGA GCTGGCCCGG 
CAGGAGACGG TACCGCACCC GCGGGCACGC GCGGGGGGGC ACTGCCACGC GCAGAGCGCT 
CTTCCCCTGG GGTCTGAGGT GGAGGCGAAC GAGAGCGAGC TGGCCCGGCA GGAGACGACA 
TTTCAGAGAC TCCAGAAAGA GAGAGAAAGT GAAGAAAAAA AGTTAGAAGC CAGTCGAGCG 
ACTCTGCAGG AGCAGCAGCA GCAGCTGGAA AAGGAAATCG CAGACCAGAA GAACGTGCTG 
GACCAGGTGC TCAGCCAGGT GCTGGGGGCC AAGGAGCGCG CCAGGACTCT GCAGCAGGAG 
GAGAGGTGGG GTGAGACCCT GGAGAAGACG CTCTCCCAGA CCCAGCAACA GCTCTCAGAA 
CGGGAGCAGC AGTTGTTGGA AAAGTCAGCT GAGCTGCTGG CTCTTCAGAA AGAGGCAGAC 
TCGATGAGGG CAGACTTCAG CCTCTTGCGA AACCAGTTCT TGACGGAGAG GAAGAAAGCC 
GAGAAGCAGG TAGCCAGCCT CAGGGAAGCG CTCAAGACCC AGCGCAGCCA GCTGGAGAAG 
ACCCTTCTCG AGCAGAAGCA GGAGAACAGC TCCATGCAGA AGGAGATGGC GACTATCGAG 
CAGGTGGCGC AGGACAACCA CGAGCGGGCC CGGCGCCTGA TGCGGGAGCT CAGCCAGATG 
CAGCAGGAGT ACGCGGAGCT CAAGAAGCAG ATGGTAAACC AGAAAGATCT GGAAAGAAGA 
CAAGTAGAAA TCAGTGAAGC GATGAGGACA CTTAAGTCTG AAGTGAAAGA CGAGATCAGA 
GCCAGCCTGA AGAACCTCAA CCAGTTTCTC CCGGAGCTGC CAGCAGACCT GGAGGCCATT 
CTGGAAAGGA GCGAACACCT AGACGGAGGG CTGGAGAGCT TGAAGGAGAA CTTTCCATTC 
GCCCTGAGTG AGAAGCCATC ACCTTTGGAA GAGAAACTAC ATTTTTCTCG CGTTCACATA 
ATGGATGAGC ACTGGCGTGG AGAAGCGCTC CGGGAGAAGC TGCGGCACCG TGAGGACCGA 
CTCAAGGCCC AGCTCCGACA CTGTATGTCC AAGCAGGCAG AGGTGTTAAG CAAAGGGAAG 
CGGCAGACCG AGGGCACGTT ACACAGCCTG AGGAGGCAGG TCGACGCCTT AGGGGAGCTG 
GTCACCAGCA CGTCTGTGGA GTCCACGTCG TCAGCCACTC TGTCTCAGCT CGAATCTTCC 
CTTGCAGAGG ACTCCCATCT TGGACCAAGT CAGAGCTCCT TCCATCAAGT TCCACAAGGT 
CCATCACAAT CAGTAGACAG TTACCGACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025138130.1
CDS	220..7392
Translation	MKKGPQQKISKTKMPPSSHSPSPSSLTSNMRSRSLSPLNGSETLPFHSGRQWYGQVEIIGENSMLLDYHDQKEADSHAGVRYITEALVKKLTKQENLTLVKSLNLSLSKDGGKKFKYIENLEKCTKLEILNLSHNLIGKIEKVDKLLKLRELNLSYNKICKIEGIENMHNLQKLNLAGNEIEHIPAWLGKKLKCLRVLNLKANKISSLQDVSKLKPLQDLTSLILAENPVVTLPHYLQFTIFHLRSLESLEGQPVTTQDRQEAFERFSLEEVERLERDLEKKMMETEELKSKQAQFLEEIKNQDKLNRSLKEEAMLQKQSCEELESNLNTKNELPLSRFLADPCLPILYSSLKLRQKTMELTRACQKQYELEQELAFYKIDAKFEPLNYYPSEYVDVDKAPDESPYIGKSRYKRNMFATESYIVNNAQPVEIKRMEPEEGEQLRNEHVSLKAHMPLDTELEDKENKISTAQARLSALHDEIEKAEQQILRATEEFKQLEEAVQLKKISEAEKDFLLKQLSGRIQLLNKLRQEALDLEMQMEKQRGEIAEKQEEIKDLQRVIDSLDSKDPKHCHMKAQKRGKEQQFDIMNKQYKQLESRLDEILSRIAKETEEIKDLEQQLTEGQIAANEALKKDLEGVISGLQEYLGTVKGQAAQAQSECRRLQDEKEALLQRLAEVEQERDQLEAVALDAEAMRKELAELESALQEQHEVNASLQQTHGDLSAYEAELEAQLRMRDAEASQLKEELGELTRLSQLEQSTLQTELEKEKQALKNALGKAQLSEEKEQENSELRTQLKQLQDDNNLLKQQLKDFQNHLNQVADGLIRPEEVAARVDEVRRKLKSGAGEMSRIHSPSDVLGKSLADLQIQFSEMLTRSQWEKEEAQGRERKLQEEMALHQEKLVDGQEAFRQACERALEARINFDKRQHEARIQQLENEIHYLQENLKSMEEIQGLTDLQLQEADEEKERILAQLQELEKKKKLEDAKSQAQVLGLDKELKKLKKAVATSDKIATAELTIAKDQLKSLHGTVMKINQERAEDLQEAERFSRKAAQAARDLTRAEAEIELLQNLLREREEQMEKVEAGTRGASSQALEIERWNETMERQRSEIARLRDSLDRTGADNRGGIENILEEIAELRREVSYQNDYISSMADPFKRRGYWYFMPPPSSSKVSSHSSQATKDSGVGMKYAASTPIRKPQQNGREDGAPPAASGYWVYSPIRSGLHKSFSNKDADSGGESREESELDDHDDRPFVPPPGYMMYTVFPDGSPVPQGMALYAPPPPLPNNSRPLSPGTVVYGPPPAGAPIVYGPPPPSFSVPLIPVGVLHCNVPEHHALENEVSRLEDIIQHLKSKKQEQRMRVCKRQSEKEIEELHHNIDELLQEKKDLEHEVEELHRTIQKHQQQKDFIDGNVESLMNELKIEKSLKHHEDIVDEIECIEKTLLKRRAELREADRLLAEAESELSRTTEKTKNAVEKFTDAKRNLLQTEKDAEELERRAQETAVSLVKADQQLRLLQADTKDLEQHKTEQEEILKEINKVVAAKDSDFQCLNTKKEKLTEELQKLQKDIETAARTEDHHLQVLRESEALLQAKRAELDKLRSQVTSQQQEMAVLDRQLGHKKEELHLLQGSMVQARADLQEALRLGETEVTEKCNHIRELKGLLEELSFQKGELNVQISEKKNQLSLIKQEIEKEEENLQVVLGQMSKHKTELKNILDMLQLENNELQALKLQHEQKMSELEKAQVEVLEEKLELENLQQTALRQKGEIEWQKQLLERDKREIERITAETRALQLCVESLCKQKEDLEEKCDSWEKKLAQTKRVLAATEENSKAKQSDLEKLELDIRKLEQELDQLNRNKLSLHRDIAAMQQQVQEKREAVNSLQEELADVQAHLNLAKQDLLHTTKRQDVLLGEQTRLQEDLQERRKKLEVCQKEEATKRQHLQALQTEMEANEAELARQETVPHPRARAGGHCHAQSALPLGSEVEANESELARQETTFQRLQKERESEEKKLEASRATLQEQQQQLEKEIADQKNVLDQVLSQVLGAKERARTLQQEERWGETLEKTLSQTQQQLSEREQQLLEKSAELLALQKEADSMRADFSLLRNQFLTERKKAEKQVASLREALKTQRSQLEKTLLEQKQENSSMQKEMATIEQVAQDNHERARRLMRELSQMQQEYAELKKQMVNQKDLERRQVEISEAMRTLKSEVKDEIRASLKNLNQFLPELPADLEAILERSEHLDGGLESLKENFPFALSEKPSPLEEKLHFSRVHIMDEHWRGEALREKLRHREDRLKAQLRHCMSKQAEVLSKGKRQTEGTLHSLRRQVDALGELVTSTSVESTSSATLSQLESSLAEDSHLGPSQSSFHQVPQGPSQSVDSYRH

Target ORF information:

RefSeq Version	XM_025282345.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centriolin (CNTRL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025282345.1

ORF Insert Sequence:

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCCCTTGTC TCGGTTTCTT 
GCGGACCCTT GTCTGCCGAT TCTTTATTCT TCCTTAAAGC TAAGACAGAA GACCATGGAA 
CTAACGCGAG CATGTCAAAA ACAGTATGAA CTGGAACAGG AGTTGGCTTT TTACAAAATC 
GATGCTAAAT TTGAGCCACT GAATTATTAT CCCTCAGAGT ATGTTGACGT TGATAAAGCC 
CCAGATGAAA GCCCTTACAT TGGCAAGTCC AGATACAAGA GAAATATGTT CGCCACAGAG 
AGTTACATTG TGAATAATGC CCAGCCAGTT GAGATCAAGA GGATGGAGCC AGAGGAAGGG 
GAGCAACTTA GAAACGAGCA TGTGAGCTTG AAAGCCCATA TGCCACTGGA CACGGAGCTG 
GAAGACAAAG AGAACAAAAT AAGTACAGCA CAAGCTCGAC TATCAGCACT GCATGATGAA 
ATAGAAAAGG CAGAACAACA AATTTTAAGA GCCACTGAAG AATTTAAACA ACTGGAAGAA 
GCTGTACAAC TTAAAAAGAT TTCAGAAGCA GAGAAAGACT TTCTTCTCAA GCAGCTGAGT 
GGCAGGATAC AGCTTCTAAA TAAATTACGC CAGGAAGCTC TTGATCTAGA AATGCAGATG 
GAAAAGCAAA GAGGAGAAAT TGCTGAAAAG CAGGAGGAAA TCAAGGACCT CCAAAGAGTT 
ATTGATAGTC TGGATTCCAA AGACCCAAAA CATTGCCATA TGAAGGCTCA GAAAAGGGGT 
AAAGAACAAC AATTTGACAT TATGAACAAG CAGTACAAAC AACTTGAGAG CCGTTTGGAC 
GAGATACTTT CTAGAATTGC TAAAGAAACT GAAGAGATTA AGGACCTTGA ACAACAGCTT 
ACTGAAGGCC AGATAGCAGC AAATGAAGCC CTGAAGAAGG ACTTAGAAGG CGTCATCAGT 
GGCCTGCAAG AATACCTGGG CACTGTGAAG GGCCAGGCCG CTCAGGCCCA GAGCGAGTGC 
AGAAGGCTGC AGGATGAGAA AGAGGCGCTG CTGCAGAGGT TGGCCGAGGT CGAGCAGGAA 
AGGGACCAGC TGGAAGCAGT CGCCTTAGAT GCAGAAGCTA TGAGGAAGGA GCTTGCAGAG 
CTAGAAAGCG CCCTCCAAGA GCAGCATGAG GTGAACGCGT CCCTGCAGCA GACCCACGGA 
GACCTCAGTG CCTACGAGGC TGAGCTAGAG GCACAGCTGA GGATGAGGGA TGCCGAAGCC 
AGCCAGCTCA AAGAAGAGCT GGGAGAACTA ACAAGGCTCA GCCAGCTAGA ACAGTCAACT 
CTACAAACAG AACTTGAGAA AGAAAAACAA GCCCTCAAGA ACGCCCTGGG AAAAGCCCAG 
CTCTCGGAAG AAAAAGAACA AGAAAACAGT GAGCTCCGCA CCCAGCTTAA ACAGCTACAG 
GATGACAATA ACCTGTTAAA ACAGCAACTT AAAGATTTCC AGAATCACCT TAATCAGGTA 
GCTGATGGTC TGATTCGTCC AGAAGAAGTG GCAGCTCGTG TAGATGAAGT AAGGAGAAAA 
TTGAAATCAG GCGCTGGAGA GATGAGCAGA ATCCACAGTC CTTCAGATGT CTTAGGGAAA 
AGTCTTGCTG ACTTGCAGAT ACAATTCAGT GAAATGCTGA CACGTTCTCA GTGGGAGAAA 
GAGGAAGCCC AAGGGAGGGA AAGAAAACTC CAAGAAGAAA TGGCTCTGCA TCAGGAGAAG 
CTGGTGGATG GACAGGAAGC GTTCAGGCAG GCCTGTGAGA GAGCGCTAGA AGCAAGGATT 
AATTTTGACA AGAGGCAACA TGAAGCAAGA ATTCAGCAGT TAGAGAATGA GATTCACTAT 
TTACAAGAAA ATCTAAAAAG TATGGAGGAA ATCCAAGGGC TTACAGATCT GCAGCTGCAG 
GAAGCTGATG AAGAGAAGGA GAGAATTCTA GCCCAACTCC AGGAGTTAGA GAAGAAGAAG 
AAACTTGAAG ATGCCAAATC TCAGGCACAG GTTCTAGGTT TAGATAAAGA ATTGAAGAAA 
CTAAAGAAGG CTGTGGCCAC CTCTGATAAG ATAGCCACAG CTGAGCTCAC CATCGCCAAA 
GACCAGCTCA AGTCCCTTCA TGGAACTGTT ATGAAAATTA ACCAGGAGCG TGCAGAGGAC 
CTGCAGGAAG CAGAGCGGTT CAGCAGAAAG GCAGCACAGG CCGCGAGGGA TCTGACCCGA 
GCGGAAGCGG AGATCGAACT CCTGCAGAAC CTCCTCAGGG AGAGAGAGGA GCAGATGGAG 
AAGGTAGAGG CAGGCACCCG AGGAGCAAGC TCACAGGCGC TGGAAATCGA GAGGTGGAAT 
GAGACCATGG AGAGACAGAG GAGCGAGATC GCAAGGCTAC GGGACTCACT AGACCGCACC 
GGGGCGGATA ACAGAGGAGG CATTGAAAAT ATTTTGGAAG AAATAGCTGA ACTCCGACGT 
GAAGTTTCTT ACCAGAATGA TTACATCAGC AGCATGGCAG ATCCTTTCAA AAGACGAGGC 
TATTGGTACT TTATGCCACC ACCATCATCA TCAAAAGTTT CCAGCCACAG CTCCCAGGCC 
ACAAAGGACT CTGGTGTCGG GATGAAGTAC GCAGCCTCGA CTCCTATTAG AAAACCACAG 
CAGAACGGGA GGGAAGACGG TGCCCCTCCA GCTGCCTCCG GATACTGGGT GTATTCCCCC 
ATCAGGAGCG GCTTACATAA ATCGTTTTCA AATAAAGATG CAGACAGTGG AGGAGAGAGC 
CGGGAGGAGA GCGAGCTGGA TGACCACGAT GACCGCCCGT TCGTCCCTCC TCCTGGATAC 
ATGATGTACA CCGTGTTCCC CGACGGCTCT CCTGTACCAC AGGGCATGGC CCTCTACGCA 
CCGCCGCCTC CCTTGCCCAA CAATAGCCGA CCTCTCAGCC CTGGCACTGT TGTGTATGGC 
CCGCCCCCTG CTGGTGCCCC CATTGTCTAC GGGCCCCCAC CGCCCAGCTT CTCCGTCCCC 
CTCATCCCAG TGGGCGTGCT GCACTGCAAC GTCCCGGAGC ACCATGCCTT AGAGAATGAG 
GTTTCTAGAT TGGAAGACAT AATACAGCAT TTAAAATCAA AGAAGCAGGA ACAGAGGATG 
AGAGTATGCA AGCGGCAGTC TGAGAAAGAA ATAGAAGAAC TGCATCATAA TATTGATGAA 
CTTTTGCAAG AGAAGAAAGA CTTAGAGCAC GAAGTAGAAG AATTACATAG AACCATCCAG 
AAACATCAAC AGCAAAAAGA CTTCATTGAT GGAAATGTTG AGAGTCTTAT GAATGAACTA 
AAAATAGAAA AATCACTCAA GCACCATGAA GATATTGTAG ATGAAATTGA GTGCATCGAG 
AAGACTCTCC TGAAACGTCG AGCAGAGCTC AGGGAGGCTG ACCGACTGCT GGCGGAAGCC 
GAGAGTGAAC TTTCACGCAC AACAGAGAAA ACAAAAAATG CTGTTGAAAA GTTCACTGAT 
GCCAAGAGGA ATTTGTTGCA AACTGAGAAA GATGCTGAGG AGTTAGAAAG GAGAGCTCAG 
GAAACAGCTG TTAGCCTTGT CAAGGCTGAT CAACAGCTCA GATTGTTACA GGCGGACACA 
AAGGATTTGG AGCAGCACAA AACTGAGCAA GAAGAGATCT TGAAAGAGAT AAACAAAGTT 
GTTGCAGCAA AAGACTCAGA CTTCCAGTGT TTAAACACGA AGAAGGAGAA GCTAACAGAA 
GAGCTGCAGA AGCTGCAGAA AGACATCGAG ACGGCAGCGC GCACGGAGGA TCACCACCTG 
CAGGTCCTGA GAGAGTCGGA GGCCCTCCTG CAGGCCAAGA GAGCGGAGCT GGACAAGCTG 
AGGAGCCAGG TAACGAGTCA GCAGCAGGAG ATGGCTGTCT TGGACAGACA GCTGGGGCAT 
AAGAAGGAGG AGCTGCATCT ACTCCAGGGA AGCATGGTCC AGGCCAGAGC TGACCTCCAG 
GAAGCGCTGA GGCTGGGAGA AACTGAAGTA ACTGAGAAGT GCAACCACAT CAGGGAACTA 
AAAGGTCTTC TGGAAGAACT GAGTTTTCAG AAAGGAGAAC TGAATGTCCA GATTAGTGAG 
AAAAAAAATC AACTTTCACT TATAAAGCAA GAAATTGAAA AAGAGGAAGA AAATCTTCAG 
GTAGTTTTAG GGCAGATGTC TAAACATAAA ACTGAACTTA AGAATATTCT GGACATGTTG 
CAACTTGAAA ACAATGAGCT GCAAGCTTTG AAGCTACAGC ATGAGCAAAA GATGTCTGAG 
TTAGAGAAGG CTCAGGTTGA AGTGCTGGAG GAGAAACTGG AGTTGGAGAA TTTGCAGCAG 
ACAGCCCTGC GACAGAAAGG GGAGATCGAG TGGCAGAAGC AGCTCTTAGA GAGGGACAAG 
CGAGAGATTG AGCGGATAAC TGCTGAGACC CGAGCATTGC AGTTGTGTGT TGAGTCTTTG 
TGCAAACAAA AGGAAGATCT TGAGGAGAAA TGTGACAGCT GGGAGAAGAA GCTGGCACAG 
ACCAAACGGG TTTTAGCAGC CACAGAAGAG AATAGCAAAG CAAAGCAATC AGACTTAGAA 
AAGTTGGAAT TGGATATCAG AAAACTGGAA CAGGAACTAG ACCAGCTAAA CAGAAACAAA 
CTCTCACTGC ACAGAGACAT CGCGGCCATG CAGCAGCAGG TCCAAGAAAA ACGAGAAGCA 
GTAAACTCAT TACAGGAGGA ACTAGCTGAT GTCCAAGCCC ATTTGAACCT AGCGAAACAG 
GACCTGCTCC ACACCACCAA GCGCCAGGAT GTCCTGCTTG GCGAGCAGAC CAGGCTGCAG 
GAGGACCTCC AGGAACGCAG AAAGAAGTTG GAAGTCTGCC AAAAAGAAGA GGCAACAAAG 
CGGCAGCACC TGCAAGCGCT TCAGACGGAG ATGGAGGCGA ACGAGGCCGA GCTGGCCCGG 
CAGGAGACGG TACCGCACCC GCGGGCACGC GCGGGGGGGC ACTGCCACGC GCAGAGCGCT 
CTTCCCCTGG GGTCTGAGGT GGAGGCGAAC GAGAGCGAGC TGGCCCGGCA GGAGACGACA 
TTTCAGAGAC TCCAGAAAGA GAGAGAAAGT GAAGAAAAAA AGTTAGAAGC CAGTCGAGCG 
ACTCTGCAGG AGCAGCAGCA GCAGCTGGAA AAGGAAATCG CAGACCAGAA GAACGTGCTG 
GACCAGGTGC TCAGCCAGGT GCTGGGGGCC AAGGAGCGCG CCAGGACTCT GCAGCAGGAG 
GAGAGGTGGG GTGAGACCCT GGAGAAGACG CTCTCCCAGA CCCAGCAACA GCTCTCAGAA 
CGGGAGCAGC AGTTGTTGGA AAAGTCAGCT GAGCTGCTGG CTCTTCAGAA AGAGGCAGAC 
TCGATGAGGG CAGACTTCAG CCTCTTGCGA AACCAGTTCT TGACGGAGAG GAAGAAAGCC 
GAGAAGCAGG TAGCCAGCCT CAGGGAAGCG CTCAAGACCC AGCGCAGCCA GCTGGAGAAG 
ACCCTTCTCG AGCAGAAGCA GGAGAACAGC TCCATGCAGA AGGAGATGGC GACTATCGAG 
CAGGTGGCGC AGGACAACCA CGAGCGGGCC CGGCGCCTGA TGCGGGAGCT CAGCCAGATG 
CAGCAGGAGT ACGCGGAGCT CAAGAAGCAG ATGGTAAACC AGAAAGATCT GGAAAGAAGA 
CAAGTAGAAA TCAGTGAAGC GATGAGGACA CTTAAGTCTG AAGTGAAAGA CGAGATCAGA 
GCCAGCCTGA AGAACCTCAA CCAGTTTCTC CCGGAGCTGC CAGCAGACCT GGAGGCCATT 
CTGGAAAGGA GCGAACACCT AGACGGAGGG CTGGAGAGCT TGAAGGAGAA CTTTCCATTC 
GCCCTGAGTG AGAAGCCATC ACCTTTGGAA GAGAAACTAC ATTTTTCTCG CGTTCACATA 
ATGGATGAGC ACTGGCGTGG AGAAGCGCTC CGGGAGAAGC TGCGGCACCG TGAGGACCGA 
CTCAAGGCCC AGCTCCGACA CTGTATGTCC AAGCAGGCAG AGGTGTTAAG CAAAGGGAAG 
CGGCAGACCG AGGGCACGTT ACACAGCCTG AGGAGGCAGG TCGACGCCTT AGGGGAGCTG 
GTCACCAGCA CGTCTGTGGA GTCCACGTCG TCAGCCACTC TGTCTCAGCT CGAATCTTCC 
CTTGCAGAGG ACTCCCATCT TGGACCAAGT CAGAGCTCCT TCCATCAAGT TCCACAAGGT 
CCATCACAAT CAGTAGACAG TTACCGACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTRL ( XM_025282344.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025138129.1 Bubalus bubalis centriolin (CNTRL), transcript variant X1, mRNA.

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CloneID	OBu63433
Clone ID Related Accession (Same CDS sequence)	XM_025282345.1 , XM_025282344.1
Accession Version	XM_025282344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	centriolin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCCCTTGTC TCGGTTTCTT 
GCGGACCCTT GTCTGCCGAT TCTTTATTCT TCCTTAAAGC TAAGACAGAA GACCATGGAA 
CTAACGCGAG CATGTCAAAA ACAGTATGAA CTGGAACAGG AGTTGGCTTT TTACAAAATC 
GATGCTAAAT TTGAGCCACT GAATTATTAT CCCTCAGAGT ATGTTGACGT TGATAAAGCC 
CCAGATGAAA GCCCTTACAT TGGCAAGTCC AGATACAAGA GAAATATGTT CGCCACAGAG 
AGTTACATTG TGAATAATGC CCAGCCAGTT GAGATCAAGA GGATGGAGCC AGAGGAAGGG 
GAGCAACTTA GAAACGAGCA TGTGAGCTTG AAAGCCCATA TGCCACTGGA CACGGAGCTG 
GAAGACAAAG AGAACAAAAT AAGTACAGCA CAAGCTCGAC TATCAGCACT GCATGATGAA 
ATAGAAAAGG CAGAACAACA AATTTTAAGA GCCACTGAAG AATTTAAACA ACTGGAAGAA 
GCTGTACAAC TTAAAAAGAT TTCAGAAGCA GAGAAAGACT TTCTTCTCAA GCAGCTGAGT 
GGCAGGATAC AGCTTCTAAA TAAATTACGC CAGGAAGCTC TTGATCTAGA AATGCAGATG 
GAAAAGCAAA GAGGAGAAAT TGCTGAAAAG CAGGAGGAAA TCAAGGACCT CCAAAGAGTT 
ATTGATAGTC TGGATTCCAA AGACCCAAAA CATTGCCATA TGAAGGCTCA GAAAAGGGGT 
AAAGAACAAC AATTTGACAT TATGAACAAG CAGTACAAAC AACTTGAGAG CCGTTTGGAC 
GAGATACTTT CTAGAATTGC TAAAGAAACT GAAGAGATTA AGGACCTTGA ACAACAGCTT 
ACTGAAGGCC AGATAGCAGC AAATGAAGCC CTGAAGAAGG ACTTAGAAGG CGTCATCAGT 
GGCCTGCAAG AATACCTGGG CACTGTGAAG GGCCAGGCCG CTCAGGCCCA GAGCGAGTGC 
AGAAGGCTGC AGGATGAGAA AGAGGCGCTG CTGCAGAGGT TGGCCGAGGT CGAGCAGGAA 
AGGGACCAGC TGGAAGCAGT CGCCTTAGAT GCAGAAGCTA TGAGGAAGGA GCTTGCAGAG 
CTAGAAAGCG CCCTCCAAGA GCAGCATGAG GTGAACGCGT CCCTGCAGCA GACCCACGGA 
GACCTCAGTG CCTACGAGGC TGAGCTAGAG GCACAGCTGA GGATGAGGGA TGCCGAAGCC 
AGCCAGCTCA AAGAAGAGCT GGGAGAACTA ACAAGGCTCA GCCAGCTAGA ACAGTCAACT 
CTACAAACAG AACTTGAGAA AGAAAAACAA GCCCTCAAGA ACGCCCTGGG AAAAGCCCAG 
CTCTCGGAAG AAAAAGAACA AGAAAACAGT GAGCTCCGCA CCCAGCTTAA ACAGCTACAG 
GATGACAATA ACCTGTTAAA ACAGCAACTT AAAGATTTCC AGAATCACCT TAATCAGGTA 
GCTGATGGTC TGATTCGTCC AGAAGAAGTG GCAGCTCGTG TAGATGAAGT AAGGAGAAAA 
TTGAAATCAG GCGCTGGAGA GATGAGCAGA ATCCACAGTC CTTCAGATGT CTTAGGGAAA 
AGTCTTGCTG ACTTGCAGAT ACAATTCAGT GAAATGCTGA CACGTTCTCA GTGGGAGAAA 
GAGGAAGCCC AAGGGAGGGA AAGAAAACTC CAAGAAGAAA TGGCTCTGCA TCAGGAGAAG 
CTGGTGGATG GACAGGAAGC GTTCAGGCAG GCCTGTGAGA GAGCGCTAGA AGCAAGGATT 
AATTTTGACA AGAGGCAACA TGAAGCAAGA ATTCAGCAGT TAGAGAATGA GATTCACTAT 
TTACAAGAAA ATCTAAAAAG TATGGAGGAA ATCCAAGGGC TTACAGATCT GCAGCTGCAG 
GAAGCTGATG AAGAGAAGGA GAGAATTCTA GCCCAACTCC AGGAGTTAGA GAAGAAGAAG 
AAACTTGAAG ATGCCAAATC TCAGGCACAG GTTCTAGGTT TAGATAAAGA ATTGAAGAAA 
CTAAAGAAGG CTGTGGCCAC CTCTGATAAG ATAGCCACAG CTGAGCTCAC CATCGCCAAA 
GACCAGCTCA AGTCCCTTCA TGGAACTGTT ATGAAAATTA ACCAGGAGCG TGCAGAGGAC 
CTGCAGGAAG CAGAGCGGTT CAGCAGAAAG GCAGCACAGG CCGCGAGGGA TCTGACCCGA 
GCGGAAGCGG AGATCGAACT CCTGCAGAAC CTCCTCAGGG AGAGAGAGGA GCAGATGGAG 
AAGGTAGAGG CAGGCACCCG AGGAGCAAGC TCACAGGCGC TGGAAATCGA GAGGTGGAAT 
GAGACCATGG AGAGACAGAG GAGCGAGATC GCAAGGCTAC GGGACTCACT AGACCGCACC 
GGGGCGGATA ACAGAGGAGG CATTGAAAAT ATTTTGGAAG AAATAGCTGA ACTCCGACGT 
GAAGTTTCTT ACCAGAATGA TTACATCAGC AGCATGGCAG ATCCTTTCAA AAGACGAGGC 
TATTGGTACT TTATGCCACC ACCATCATCA TCAAAAGTTT CCAGCCACAG CTCCCAGGCC 
ACAAAGGACT CTGGTGTCGG GATGAAGTAC GCAGCCTCGA CTCCTATTAG AAAACCACAG 
CAGAACGGGA GGGAAGACGG TGCCCCTCCA GCTGCCTCCG GATACTGGGT GTATTCCCCC 
ATCAGGAGCG GCTTACATAA ATCGTTTTCA AATAAAGATG CAGACAGTGG AGGAGAGAGC 
CGGGAGGAGA GCGAGCTGGA TGACCACGAT GACCGCCCGT TCGTCCCTCC TCCTGGATAC 
ATGATGTACA CCGTGTTCCC CGACGGCTCT CCTGTACCAC AGGGCATGGC CCTCTACGCA 
CCGCCGCCTC CCTTGCCCAA CAATAGCCGA CCTCTCAGCC CTGGCACTGT TGTGTATGGC 
CCGCCCCCTG CTGGTGCCCC CATTGTCTAC GGGCCCCCAC CGCCCAGCTT CTCCGTCCCC 
CTCATCCCAG TGGGCGTGCT GCACTGCAAC GTCCCGGAGC ACCATGCCTT AGAGAATGAG 
GTTTCTAGAT TGGAAGACAT AATACAGCAT TTAAAATCAA AGAAGCAGGA ACAGAGGATG 
AGAGTATGCA AGCGGCAGTC TGAGAAAGAA ATAGAAGAAC TGCATCATAA TATTGATGAA 
CTTTTGCAAG AGAAGAAAGA CTTAGAGCAC GAAGTAGAAG AATTACATAG AACCATCCAG 
AAACATCAAC AGCAAAAAGA CTTCATTGAT GGAAATGTTG AGAGTCTTAT GAATGAACTA 
AAAATAGAAA AATCACTCAA GCACCATGAA GATATTGTAG ATGAAATTGA GTGCATCGAG 
AAGACTCTCC TGAAACGTCG AGCAGAGCTC AGGGAGGCTG ACCGACTGCT GGCGGAAGCC 
GAGAGTGAAC TTTCACGCAC AACAGAGAAA ACAAAAAATG CTGTTGAAAA GTTCACTGAT 
GCCAAGAGGA ATTTGTTGCA AACTGAGAAA GATGCTGAGG AGTTAGAAAG GAGAGCTCAG 
GAAACAGCTG TTAGCCTTGT CAAGGCTGAT CAACAGCTCA GATTGTTACA GGCGGACACA 
AAGGATTTGG AGCAGCACAA AACTGAGCAA GAAGAGATCT TGAAAGAGAT AAACAAAGTT 
GTTGCAGCAA AAGACTCAGA CTTCCAGTGT TTAAACACGA AGAAGGAGAA GCTAACAGAA 
GAGCTGCAGA AGCTGCAGAA AGACATCGAG ACGGCAGCGC GCACGGAGGA TCACCACCTG 
CAGGTCCTGA GAGAGTCGGA GGCCCTCCTG CAGGCCAAGA GAGCGGAGCT GGACAAGCTG 
AGGAGCCAGG TAACGAGTCA GCAGCAGGAG ATGGCTGTCT TGGACAGACA GCTGGGGCAT 
AAGAAGGAGG AGCTGCATCT ACTCCAGGGA AGCATGGTCC AGGCCAGAGC TGACCTCCAG 
GAAGCGCTGA GGCTGGGAGA AACTGAAGTA ACTGAGAAGT GCAACCACAT CAGGGAACTA 
AAAGGTCTTC TGGAAGAACT GAGTTTTCAG AAAGGAGAAC TGAATGTCCA GATTAGTGAG 
AAAAAAAATC AACTTTCACT TATAAAGCAA GAAATTGAAA AAGAGGAAGA AAATCTTCAG 
GTAGTTTTAG GGCAGATGTC TAAACATAAA ACTGAACTTA AGAATATTCT GGACATGTTG 
CAACTTGAAA ACAATGAGCT GCAAGCTTTG AAGCTACAGC ATGAGCAAAA GATGTCTGAG 
TTAGAGAAGG CTCAGGTTGA AGTGCTGGAG GAGAAACTGG AGTTGGAGAA TTTGCAGCAG 
ACAGCCCTGC GACAGAAAGG GGAGATCGAG TGGCAGAAGC AGCTCTTAGA GAGGGACAAG 
CGAGAGATTG AGCGGATAAC TGCTGAGACC CGAGCATTGC AGTTGTGTGT TGAGTCTTTG 
TGCAAACAAA AGGAAGATCT TGAGGAGAAA TGTGACAGCT GGGAGAAGAA GCTGGCACAG 
ACCAAACGGG TTTTAGCAGC CACAGAAGAG AATAGCAAAG CAAAGCAATC AGACTTAGAA 
AAGTTGGAAT TGGATATCAG AAAACTGGAA CAGGAACTAG ACCAGCTAAA CAGAAACAAA 
CTCTCACTGC ACAGAGACAT CGCGGCCATG CAGCAGCAGG TCCAAGAAAA ACGAGAAGCA 
GTAAACTCAT TACAGGAGGA ACTAGCTGAT GTCCAAGCCC ATTTGAACCT AGCGAAACAG 
GACCTGCTCC ACACCACCAA GCGCCAGGAT GTCCTGCTTG GCGAGCAGAC CAGGCTGCAG 
GAGGACCTCC AGGAACGCAG AAAGAAGTTG GAAGTCTGCC AAAAAGAAGA GGCAACAAAG 
CGGCAGCACC TGCAAGCGCT TCAGACGGAG ATGGAGGCGA ACGAGGCCGA GCTGGCCCGG 
CAGGAGACGG TACCGCACCC GCGGGCACGC GCGGGGGGGC ACTGCCACGC GCAGAGCGCT 
CTTCCCCTGG GGTCTGAGGT GGAGGCGAAC GAGAGCGAGC TGGCCCGGCA GGAGACGACA 
TTTCAGAGAC TCCAGAAAGA GAGAGAAAGT GAAGAAAAAA AGTTAGAAGC CAGTCGAGCG 
ACTCTGCAGG AGCAGCAGCA GCAGCTGGAA AAGGAAATCG CAGACCAGAA GAACGTGCTG 
GACCAGGTGC TCAGCCAGGT GCTGGGGGCC AAGGAGCGCG CCAGGACTCT GCAGCAGGAG 
GAGAGGTGGG GTGAGACCCT GGAGAAGACG CTCTCCCAGA CCCAGCAACA GCTCTCAGAA 
CGGGAGCAGC AGTTGTTGGA AAAGTCAGCT GAGCTGCTGG CTCTTCAGAA AGAGGCAGAC 
TCGATGAGGG CAGACTTCAG CCTCTTGCGA AACCAGTTCT TGACGGAGAG GAAGAAAGCC 
GAGAAGCAGG TAGCCAGCCT CAGGGAAGCG CTCAAGACCC AGCGCAGCCA GCTGGAGAAG 
ACCCTTCTCG AGCAGAAGCA GGAGAACAGC TCCATGCAGA AGGAGATGGC GACTATCGAG 
CAGGTGGCGC AGGACAACCA CGAGCGGGCC CGGCGCCTGA TGCGGGAGCT CAGCCAGATG 
CAGCAGGAGT ACGCGGAGCT CAAGAAGCAG ATGGTAAACC AGAAAGATCT GGAAAGAAGA 
CAAGTAGAAA TCAGTGAAGC GATGAGGACA CTTAAGTCTG AAGTGAAAGA CGAGATCAGA 
GCCAGCCTGA AGAACCTCAA CCAGTTTCTC CCGGAGCTGC CAGCAGACCT GGAGGCCATT 
CTGGAAAGGA GCGAACACCT AGACGGAGGG CTGGAGAGCT TGAAGGAGAA CTTTCCATTC 
GCCCTGAGTG AGAAGCCATC ACCTTTGGAA GAGAAACTAC ATTTTTCTCG CGTTCACATA 
ATGGATGAGC ACTGGCGTGG AGAAGCGCTC CGGGAGAAGC TGCGGCACCG TGAGGACCGA 
CTCAAGGCCC AGCTCCGACA CTGTATGTCC AAGCAGGCAG AGGTGTTAAG CAAAGGGAAG 
CGGCAGACCG AGGGCACGTT ACACAGCCTG AGGAGGCAGG TCGACGCCTT AGGGGAGCTG 
GTCACCAGCA CGTCTGTGGA GTCCACGTCG TCAGCCACTC TGTCTCAGCT CGAATCTTCC 
CTTGCAGAGG ACTCCCATCT TGGACCAAGT CAGAGCTCCT TCCATCAAGT TCCACAAGGT 
CCATCACAAT CAGTAGACAG TTACCGACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025138129.1
CDS	359..7531
Translation	MKKGPQQKISKTKMPPSSHSPSPSSLTSNMRSRSLSPLNGSETLPFHSGRQWYGQVEIIGENSMLLDYHDQKEADSHAGVRYITEALVKKLTKQENLTLVKSLNLSLSKDGGKKFKYIENLEKCTKLEILNLSHNLIGKIEKVDKLLKLRELNLSYNKICKIEGIENMHNLQKLNLAGNEIEHIPAWLGKKLKCLRVLNLKANKISSLQDVSKLKPLQDLTSLILAENPVVTLPHYLQFTIFHLRSLESLEGQPVTTQDRQEAFERFSLEEVERLERDLEKKMMETEELKSKQAQFLEEIKNQDKLNRSLKEEAMLQKQSCEELESNLNTKNELPLSRFLADPCLPILYSSLKLRQKTMELTRACQKQYELEQELAFYKIDAKFEPLNYYPSEYVDVDKAPDESPYIGKSRYKRNMFATESYIVNNAQPVEIKRMEPEEGEQLRNEHVSLKAHMPLDTELEDKENKISTAQARLSALHDEIEKAEQQILRATEEFKQLEEAVQLKKISEAEKDFLLKQLSGRIQLLNKLRQEALDLEMQMEKQRGEIAEKQEEIKDLQRVIDSLDSKDPKHCHMKAQKRGKEQQFDIMNKQYKQLESRLDEILSRIAKETEEIKDLEQQLTEGQIAANEALKKDLEGVISGLQEYLGTVKGQAAQAQSECRRLQDEKEALLQRLAEVEQERDQLEAVALDAEAMRKELAELESALQEQHEVNASLQQTHGDLSAYEAELEAQLRMRDAEASQLKEELGELTRLSQLEQSTLQTELEKEKQALKNALGKAQLSEEKEQENSELRTQLKQLQDDNNLLKQQLKDFQNHLNQVADGLIRPEEVAARVDEVRRKLKSGAGEMSRIHSPSDVLGKSLADLQIQFSEMLTRSQWEKEEAQGRERKLQEEMALHQEKLVDGQEAFRQACERALEARINFDKRQHEARIQQLENEIHYLQENLKSMEEIQGLTDLQLQEADEEKERILAQLQELEKKKKLEDAKSQAQVLGLDKELKKLKKAVATSDKIATAELTIAKDQLKSLHGTVMKINQERAEDLQEAERFSRKAAQAARDLTRAEAEIELLQNLLREREEQMEKVEAGTRGASSQALEIERWNETMERQRSEIARLRDSLDRTGADNRGGIENILEEIAELRREVSYQNDYISSMADPFKRRGYWYFMPPPSSSKVSSHSSQATKDSGVGMKYAASTPIRKPQQNGREDGAPPAASGYWVYSPIRSGLHKSFSNKDADSGGESREESELDDHDDRPFVPPPGYMMYTVFPDGSPVPQGMALYAPPPPLPNNSRPLSPGTVVYGPPPAGAPIVYGPPPPSFSVPLIPVGVLHCNVPEHHALENEVSRLEDIIQHLKSKKQEQRMRVCKRQSEKEIEELHHNIDELLQEKKDLEHEVEELHRTIQKHQQQKDFIDGNVESLMNELKIEKSLKHHEDIVDEIECIEKTLLKRRAELREADRLLAEAESELSRTTEKTKNAVEKFTDAKRNLLQTEKDAEELERRAQETAVSLVKADQQLRLLQADTKDLEQHKTEQEEILKEINKVVAAKDSDFQCLNTKKEKLTEELQKLQKDIETAARTEDHHLQVLRESEALLQAKRAELDKLRSQVTSQQQEMAVLDRQLGHKKEELHLLQGSMVQARADLQEALRLGETEVTEKCNHIRELKGLLEELSFQKGELNVQISEKKNQLSLIKQEIEKEEENLQVVLGQMSKHKTELKNILDMLQLENNELQALKLQHEQKMSELEKAQVEVLEEKLELENLQQTALRQKGEIEWQKQLLERDKREIERITAETRALQLCVESLCKQKEDLEEKCDSWEKKLAQTKRVLAATEENSKAKQSDLEKLELDIRKLEQELDQLNRNKLSLHRDIAAMQQQVQEKREAVNSLQEELADVQAHLNLAKQDLLHTTKRQDVLLGEQTRLQEDLQERRKKLEVCQKEEATKRQHLQALQTEMEANEAELARQETVPHPRARAGGHCHAQSALPLGSEVEANESELARQETTFQRLQKERESEEKKLEASRATLQEQQQQLEKEIADQKNVLDQVLSQVLGAKERARTLQQEERWGETLEKTLSQTQQQLSEREQQLLEKSAELLALQKEADSMRADFSLLRNQFLTERKKAEKQVASLREALKTQRSQLEKTLLEQKQENSSMQKEMATIEQVAQDNHERARRLMRELSQMQQEYAELKKQMVNQKDLERRQVEISEAMRTLKSEVKDEIRASLKNLNQFLPELPADLEAILERSEHLDGGLESLKENFPFALSEKPSPLEEKLHFSRVHIMDEHWRGEALREKLRHREDRLKAQLRHCMSKQAEVLSKGKRQTEGTLHSLRRQVDALGELVTSTSVESTSSATLSQLESSLAEDSHLGPSQSSFHQVPQGPSQSVDSYRH

Target ORF information:

RefSeq Version	XM_025282344.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centriolin (CNTRL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025282344.1

ORF Insert Sequence:

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCCCTTGTC TCGGTTTCTT 
GCGGACCCTT GTCTGCCGAT TCTTTATTCT TCCTTAAAGC TAAGACAGAA GACCATGGAA 
CTAACGCGAG CATGTCAAAA ACAGTATGAA CTGGAACAGG AGTTGGCTTT TTACAAAATC 
GATGCTAAAT TTGAGCCACT GAATTATTAT CCCTCAGAGT ATGTTGACGT TGATAAAGCC 
CCAGATGAAA GCCCTTACAT TGGCAAGTCC AGATACAAGA GAAATATGTT CGCCACAGAG 
AGTTACATTG TGAATAATGC CCAGCCAGTT GAGATCAAGA GGATGGAGCC AGAGGAAGGG 
GAGCAACTTA GAAACGAGCA TGTGAGCTTG AAAGCCCATA TGCCACTGGA CACGGAGCTG 
GAAGACAAAG AGAACAAAAT AAGTACAGCA CAAGCTCGAC TATCAGCACT GCATGATGAA 
ATAGAAAAGG CAGAACAACA AATTTTAAGA GCCACTGAAG AATTTAAACA ACTGGAAGAA 
GCTGTACAAC TTAAAAAGAT TTCAGAAGCA GAGAAAGACT TTCTTCTCAA GCAGCTGAGT 
GGCAGGATAC AGCTTCTAAA TAAATTACGC CAGGAAGCTC TTGATCTAGA AATGCAGATG 
GAAAAGCAAA GAGGAGAAAT TGCTGAAAAG CAGGAGGAAA TCAAGGACCT CCAAAGAGTT 
ATTGATAGTC TGGATTCCAA AGACCCAAAA CATTGCCATA TGAAGGCTCA GAAAAGGGGT 
AAAGAACAAC AATTTGACAT TATGAACAAG CAGTACAAAC AACTTGAGAG CCGTTTGGAC 
GAGATACTTT CTAGAATTGC TAAAGAAACT GAAGAGATTA AGGACCTTGA ACAACAGCTT 
ACTGAAGGCC AGATAGCAGC AAATGAAGCC CTGAAGAAGG ACTTAGAAGG CGTCATCAGT 
GGCCTGCAAG AATACCTGGG CACTGTGAAG GGCCAGGCCG CTCAGGCCCA GAGCGAGTGC 
AGAAGGCTGC AGGATGAGAA AGAGGCGCTG CTGCAGAGGT TGGCCGAGGT CGAGCAGGAA 
AGGGACCAGC TGGAAGCAGT CGCCTTAGAT GCAGAAGCTA TGAGGAAGGA GCTTGCAGAG 
CTAGAAAGCG CCCTCCAAGA GCAGCATGAG GTGAACGCGT CCCTGCAGCA GACCCACGGA 
GACCTCAGTG CCTACGAGGC TGAGCTAGAG GCACAGCTGA GGATGAGGGA TGCCGAAGCC 
AGCCAGCTCA AAGAAGAGCT GGGAGAACTA ACAAGGCTCA GCCAGCTAGA ACAGTCAACT 
CTACAAACAG AACTTGAGAA AGAAAAACAA GCCCTCAAGA ACGCCCTGGG AAAAGCCCAG 
CTCTCGGAAG AAAAAGAACA AGAAAACAGT GAGCTCCGCA CCCAGCTTAA ACAGCTACAG 
GATGACAATA ACCTGTTAAA ACAGCAACTT AAAGATTTCC AGAATCACCT TAATCAGGTA 
GCTGATGGTC TGATTCGTCC AGAAGAAGTG GCAGCTCGTG TAGATGAAGT AAGGAGAAAA 
TTGAAATCAG GCGCTGGAGA GATGAGCAGA ATCCACAGTC CTTCAGATGT CTTAGGGAAA 
AGTCTTGCTG ACTTGCAGAT ACAATTCAGT GAAATGCTGA CACGTTCTCA GTGGGAGAAA 
GAGGAAGCCC AAGGGAGGGA AAGAAAACTC CAAGAAGAAA TGGCTCTGCA TCAGGAGAAG 
CTGGTGGATG GACAGGAAGC GTTCAGGCAG GCCTGTGAGA GAGCGCTAGA AGCAAGGATT 
AATTTTGACA AGAGGCAACA TGAAGCAAGA ATTCAGCAGT TAGAGAATGA GATTCACTAT 
TTACAAGAAA ATCTAAAAAG TATGGAGGAA ATCCAAGGGC TTACAGATCT GCAGCTGCAG 
GAAGCTGATG AAGAGAAGGA GAGAATTCTA GCCCAACTCC AGGAGTTAGA GAAGAAGAAG 
AAACTTGAAG ATGCCAAATC TCAGGCACAG GTTCTAGGTT TAGATAAAGA ATTGAAGAAA 
CTAAAGAAGG CTGTGGCCAC CTCTGATAAG ATAGCCACAG CTGAGCTCAC CATCGCCAAA 
GACCAGCTCA AGTCCCTTCA TGGAACTGTT ATGAAAATTA ACCAGGAGCG TGCAGAGGAC 
CTGCAGGAAG CAGAGCGGTT CAGCAGAAAG GCAGCACAGG CCGCGAGGGA TCTGACCCGA 
GCGGAAGCGG AGATCGAACT CCTGCAGAAC CTCCTCAGGG AGAGAGAGGA GCAGATGGAG 
AAGGTAGAGG CAGGCACCCG AGGAGCAAGC TCACAGGCGC TGGAAATCGA GAGGTGGAAT 
GAGACCATGG AGAGACAGAG GAGCGAGATC GCAAGGCTAC GGGACTCACT AGACCGCACC 
GGGGCGGATA ACAGAGGAGG CATTGAAAAT ATTTTGGAAG AAATAGCTGA ACTCCGACGT 
GAAGTTTCTT ACCAGAATGA TTACATCAGC AGCATGGCAG ATCCTTTCAA AAGACGAGGC 
TATTGGTACT TTATGCCACC ACCATCATCA TCAAAAGTTT CCAGCCACAG CTCCCAGGCC 
ACAAAGGACT CTGGTGTCGG GATGAAGTAC GCAGCCTCGA CTCCTATTAG AAAACCACAG 
CAGAACGGGA GGGAAGACGG TGCCCCTCCA GCTGCCTCCG GATACTGGGT GTATTCCCCC 
ATCAGGAGCG GCTTACATAA ATCGTTTTCA AATAAAGATG CAGACAGTGG AGGAGAGAGC 
CGGGAGGAGA GCGAGCTGGA TGACCACGAT GACCGCCCGT TCGTCCCTCC TCCTGGATAC 
ATGATGTACA CCGTGTTCCC CGACGGCTCT CCTGTACCAC AGGGCATGGC CCTCTACGCA 
CCGCCGCCTC CCTTGCCCAA CAATAGCCGA CCTCTCAGCC CTGGCACTGT TGTGTATGGC 
CCGCCCCCTG CTGGTGCCCC CATTGTCTAC GGGCCCCCAC CGCCCAGCTT CTCCGTCCCC 
CTCATCCCAG TGGGCGTGCT GCACTGCAAC GTCCCGGAGC ACCATGCCTT AGAGAATGAG 
GTTTCTAGAT TGGAAGACAT AATACAGCAT TTAAAATCAA AGAAGCAGGA ACAGAGGATG 
AGAGTATGCA AGCGGCAGTC TGAGAAAGAA ATAGAAGAAC TGCATCATAA TATTGATGAA 
CTTTTGCAAG AGAAGAAAGA CTTAGAGCAC GAAGTAGAAG AATTACATAG AACCATCCAG 
AAACATCAAC AGCAAAAAGA CTTCATTGAT GGAAATGTTG AGAGTCTTAT GAATGAACTA 
AAAATAGAAA AATCACTCAA GCACCATGAA GATATTGTAG ATGAAATTGA GTGCATCGAG 
AAGACTCTCC TGAAACGTCG AGCAGAGCTC AGGGAGGCTG ACCGACTGCT GGCGGAAGCC 
GAGAGTGAAC TTTCACGCAC AACAGAGAAA ACAAAAAATG CTGTTGAAAA GTTCACTGAT 
GCCAAGAGGA ATTTGTTGCA AACTGAGAAA GATGCTGAGG AGTTAGAAAG GAGAGCTCAG 
GAAACAGCTG TTAGCCTTGT CAAGGCTGAT CAACAGCTCA GATTGTTACA GGCGGACACA 
AAGGATTTGG AGCAGCACAA AACTGAGCAA GAAGAGATCT TGAAAGAGAT AAACAAAGTT 
GTTGCAGCAA AAGACTCAGA CTTCCAGTGT TTAAACACGA AGAAGGAGAA GCTAACAGAA 
GAGCTGCAGA AGCTGCAGAA AGACATCGAG ACGGCAGCGC GCACGGAGGA TCACCACCTG 
CAGGTCCTGA GAGAGTCGGA GGCCCTCCTG CAGGCCAAGA GAGCGGAGCT GGACAAGCTG 
AGGAGCCAGG TAACGAGTCA GCAGCAGGAG ATGGCTGTCT TGGACAGACA GCTGGGGCAT 
AAGAAGGAGG AGCTGCATCT ACTCCAGGGA AGCATGGTCC AGGCCAGAGC TGACCTCCAG 
GAAGCGCTGA GGCTGGGAGA AACTGAAGTA ACTGAGAAGT GCAACCACAT CAGGGAACTA 
AAAGGTCTTC TGGAAGAACT GAGTTTTCAG AAAGGAGAAC TGAATGTCCA GATTAGTGAG 
AAAAAAAATC AACTTTCACT TATAAAGCAA GAAATTGAAA AAGAGGAAGA AAATCTTCAG 
GTAGTTTTAG GGCAGATGTC TAAACATAAA ACTGAACTTA AGAATATTCT GGACATGTTG 
CAACTTGAAA ACAATGAGCT GCAAGCTTTG AAGCTACAGC ATGAGCAAAA GATGTCTGAG 
TTAGAGAAGG CTCAGGTTGA AGTGCTGGAG GAGAAACTGG AGTTGGAGAA TTTGCAGCAG 
ACAGCCCTGC GACAGAAAGG GGAGATCGAG TGGCAGAAGC AGCTCTTAGA GAGGGACAAG 
CGAGAGATTG AGCGGATAAC TGCTGAGACC CGAGCATTGC AGTTGTGTGT TGAGTCTTTG 
TGCAAACAAA AGGAAGATCT TGAGGAGAAA TGTGACAGCT GGGAGAAGAA GCTGGCACAG 
ACCAAACGGG TTTTAGCAGC CACAGAAGAG AATAGCAAAG CAAAGCAATC AGACTTAGAA 
AAGTTGGAAT TGGATATCAG AAAACTGGAA CAGGAACTAG ACCAGCTAAA CAGAAACAAA 
CTCTCACTGC ACAGAGACAT CGCGGCCATG CAGCAGCAGG TCCAAGAAAA ACGAGAAGCA 
GTAAACTCAT TACAGGAGGA ACTAGCTGAT GTCCAAGCCC ATTTGAACCT AGCGAAACAG 
GACCTGCTCC ACACCACCAA GCGCCAGGAT GTCCTGCTTG GCGAGCAGAC CAGGCTGCAG 
GAGGACCTCC AGGAACGCAG AAAGAAGTTG GAAGTCTGCC AAAAAGAAGA GGCAACAAAG 
CGGCAGCACC TGCAAGCGCT TCAGACGGAG ATGGAGGCGA ACGAGGCCGA GCTGGCCCGG 
CAGGAGACGG TACCGCACCC GCGGGCACGC GCGGGGGGGC ACTGCCACGC GCAGAGCGCT 
CTTCCCCTGG GGTCTGAGGT GGAGGCGAAC GAGAGCGAGC TGGCCCGGCA GGAGACGACA 
TTTCAGAGAC TCCAGAAAGA GAGAGAAAGT GAAGAAAAAA AGTTAGAAGC CAGTCGAGCG 
ACTCTGCAGG AGCAGCAGCA GCAGCTGGAA AAGGAAATCG CAGACCAGAA GAACGTGCTG 
GACCAGGTGC TCAGCCAGGT GCTGGGGGCC AAGGAGCGCG CCAGGACTCT GCAGCAGGAG 
GAGAGGTGGG GTGAGACCCT GGAGAAGACG CTCTCCCAGA CCCAGCAACA GCTCTCAGAA 
CGGGAGCAGC AGTTGTTGGA AAAGTCAGCT GAGCTGCTGG CTCTTCAGAA AGAGGCAGAC 
TCGATGAGGG CAGACTTCAG CCTCTTGCGA AACCAGTTCT TGACGGAGAG GAAGAAAGCC 
GAGAAGCAGG TAGCCAGCCT CAGGGAAGCG CTCAAGACCC AGCGCAGCCA GCTGGAGAAG 
ACCCTTCTCG AGCAGAAGCA GGAGAACAGC TCCATGCAGA AGGAGATGGC GACTATCGAG 
CAGGTGGCGC AGGACAACCA CGAGCGGGCC CGGCGCCTGA TGCGGGAGCT CAGCCAGATG 
CAGCAGGAGT ACGCGGAGCT CAAGAAGCAG ATGGTAAACC AGAAAGATCT GGAAAGAAGA 
CAAGTAGAAA TCAGTGAAGC GATGAGGACA CTTAAGTCTG AAGTGAAAGA CGAGATCAGA 
GCCAGCCTGA AGAACCTCAA CCAGTTTCTC CCGGAGCTGC CAGCAGACCT GGAGGCCATT 
CTGGAAAGGA GCGAACACCT AGACGGAGGG CTGGAGAGCT TGAAGGAGAA CTTTCCATTC 
GCCCTGAGTG AGAAGCCATC ACCTTTGGAA GAGAAACTAC ATTTTTCTCG CGTTCACATA 
ATGGATGAGC ACTGGCGTGG AGAAGCGCTC CGGGAGAAGC TGCGGCACCG TGAGGACCGA 
CTCAAGGCCC AGCTCCGACA CTGTATGTCC AAGCAGGCAG AGGTGTTAAG CAAAGGGAAG 
CGGCAGACCG AGGGCACGTT ACACAGCCTG AGGAGGCAGG TCGACGCCTT AGGGGAGCTG 
GTCACCAGCA CGTCTGTGGA GTCCACGTCG TCAGCCACTC TGTCTCAGCT CGAATCTTCC 
CTTGCAGAGG ACTCCCATCT TGGACCAAGT CAGAGCTCCT TCCATCAAGT TCCACAAGGT 
CCATCACAAT CAGTAGACAG TTACCGACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTRL ( XM_025282346.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025138131.1 Bubalus bubalis centriolin (CNTRL), transcript variant X4, mRNA.

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CloneID	OBu63434
Clone ID Related Accession (Same CDS sequence)	XM_025282346.1
Accession Version	XM_025282346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	centriolin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCCCTTGTC TCGGTTTCTT 
GCGGACCCTT GTCTGCCGAT TCTTTATTCT TCCTTAAAGC TAAGACAGAA GACCATGGAA 
CTAACGCGAG CATGTCAAAA ACAGTATGAA CTGGAACAGG AGTTGGCTTT TTACAAAATC 
GATGCTAAAT TTGAGCCACT GAATTATTAT CCCTCAGAGT ATGTTGACGT TGATAAAGCC 
CCAGATGAAA GCCCTTACAT TGGCAAGTCC AGATACAAGA GAAATATGTT CGCCACAGAG 
AGTTACATTG TGAATAATGC CCAGCCAGTT GAGATCAAGA GGATGGAGCC AGAGGAAGGG 
GAGCAACTTA GAAACGAGCA TGTGAGCTTG AAAGCCCATA TGCCACTGGA CACGGAGCTG 
GAAGACAAAG AGAACAAAAT AAGTACAGCA CAAGCTCGAC TATCAGCACT GCATGATGAA 
ATAGAAAAGG CAGAACAACA AATTTTAAGA GCCACTGAAG AATTTAAACA ACTGGAAGAA 
GCTGTACAAC TTAAAAAGAT TTCAGAAGCA GAGAAAGACT TTCTTCTCAA GCAGCTGAGT 
GGCAGGATAC AGCTTCTAAA TAAATTACGC CAGGAAGCTC TTGATCTAGA AATGCAGATG 
GAAAAGCAAA GAGGAGAAAT TGCTGAAAAG CAGGAGGAAA TCAAGGACCT CCAAAGAGTT 
ATTGATAGTC TGGATTCCAA AGACCCAAAA CATTGCCATA TGAAGGCTCA GAAAAGGGGT 
AAAGAACAAC AATTTGACAT TATGAACAAG CAGTACAAAC AACTTGAGAG CCGTTTGGAC 
GAGATACTTT CTAGAATTGC TAAAGAAACT GAAGAGATTA AGGACCTTGA ACAACAGCTT 
ACTGAAGGCC AGATAGCAGC AAATGAAGCC CTGAAGAAGG ACTTAGAAGG CGTCATCAGT 
GGCCTGCAAG AATACCTGGG CACTGTGAAG GGCCAGGCCG CTCAGGCCCA GAGCGAGTGC 
AGAAGGCTGC AGGATGAGAA AGAGGCGCTG CTGCAGAGGT TGGCCGAGGT CGAGCAGGAA 
AGGGACCAGC TGGAAGCAGT CGCCTTAGAT GCAGAAGCTA TGAGGAAGGA GCTTGCAGAG 
CTAGAAAGCG CCCTCCAAGA GCAGCATGAG GTGAACGCGT CCCTGCAGCA GACCCACGGA 
GACCTCAGTG CCTACGAGGC TGAGCTAGAG GCACAGCTGA GGATGAGGGA TGCCGAAGCC 
AGCCAGCTCA AAGAAGAGCT GGGAGAACTA ACAAGGCTCA GCCAGCTAGA ACAGTCAACT 
CTACAAACAG AACTTGAGAA AGAAAAACAA GCCCTCAAGA ACGCCCTGGG AAAAGCCCAG 
CTCTCGGAAG AAAAAGAACA AGAAAACAGT GAGCTCCGCA CCCAGCTTAA ACAGCTACAG 
GATGACAATA ACCTGTTAAA ACAGCAACTT AAAGATTTCC AGAATCACCT TAATCAGGTA 
GCTGATGGTC TGATTCGTCC AGAAGAAGTG GCAGCTCGTG TAGATGAAGT AAGGAGAAAA 
TTGAAATCAG GCGCTGGAGA GATGAGAATC CACAGTCCTT CAGATGTCTT AGGGAAAAGT 
CTTGCTGACT TGCAGATACA ATTCAGTGAA ATGCTGACAC GTTCTCAGTG GGAGAAAGAG 
GAAGCCCAAG GGAGGGAAAG AAAACTCCAA GAAGAAATGG CTCTGCATCA GGAGAAGCTG 
GTGGATGGAC AGGAAGCGTT CAGGCAGGCC TGTGAGAGAG CGCTAGAAGC AAGGATTAAT 
TTTGACAAGA GGCAACATGA AGCAAGAATT CAGCAGTTAG AGAATGAGAT TCACTATTTA 
CAAGAAAATC TAAAAAGTAT GGAGGAAATC CAAGGGCTTA CAGATCTGCA GCTGCAGGAA 
GCTGATGAAG AGAAGGAGAG AATTCTAGCC CAACTCCAGG AGTTAGAGAA GAAGAAGAAA 
CTTGAAGATG CCAAATCTCA GGCACAGGTT CTAGGTTTAG ATAAAGAATT GAAGAAACTA 
AAGAAGGCTG TGGCCACCTC TGATAAGATA GCCACAGCTG AGCTCACCAT CGCCAAAGAC 
CAGCTCAAGT CCCTTCATGG AACTGTTATG AAAATTAACC AGGAGCGTGC AGAGGACCTG 
CAGGAAGCAG AGCGGTTCAG CAGAAAGGCA GCACAGGCCG CGAGGGATCT GACCCGAGCG 
GAAGCGGAGA TCGAACTCCT GCAGAACCTC CTCAGGGAGA GAGAGGAGCA GATGGAGAAG 
GTAGAGGCAG GCACCCGAGG AGCAAGCTCA CAGGCGCTGG AAATCGAGAG GTGGAATGAG 
ACCATGGAGA GACAGAGGAG CGAGATCGCA AGGCTACGGG ACTCACTAGA CCGCACCGGG 
GCGGATAACA GAGGAGGCAT TGAAAATATT TTGGAAGAAA TAGCTGAACT CCGACGTGAA 
GTTTCTTACC AGAATGATTA CATCAGCAGC ATGGCAGATC CTTTCAAAAG ACGAGGCTAT 
TGGTACTTTA TGCCACCACC ATCATCATCA AAAGTTTCCA GCCACAGCTC CCAGGCCACA 
AAGGACTCTG GTGTCGGGAT GAAGTACGCA GCCTCGACTC CTATTAGAAA ACCACAGCAG 
AACGGGAGGG AAGACGGTGC CCCTCCAGCT GCCTCCGGAT ACTGGGTGTA TTCCCCCATC 
AGGAGCGGCT TACATAAATC GTTTTCAAAT AAAGATGCAG ACAGTGGAGG AGAGAGCCGG 
GAGGAGAGCG AGCTGGATGA CCACGATGAC CGCCCGTTCG TCCCTCCTCC TGGATACATG 
ATGTACACCG TGTTCCCCGA CGGCTCTCCT GTACCACAGG GCATGGCCCT CTACGCACCG 
CCGCCTCCCT TGCCCAACAA TAGCCGACCT CTCAGCCCTG GCACTGTTGT GTATGGCCCG 
CCCCCTGCTG GTGCCCCCAT TGTCTACGGG CCCCCACCGC CCAGCTTCTC CGTCCCCCTC 
ATCCCAGTGG GCGTGCTGCA CTGCAACGTC CCGGAGCACC ATGCCTTAGA GAATGAGGTT 
TCTAGATTGG AAGACATAAT ACAGCATTTA AAATCAAAGA AGCAGGAACA GAGGATGAGA 
GTATGCAAGC GGCAGTCTGA GAAAGAAATA GAAGAACTGC ATCATAATAT TGATGAACTT 
TTGCAAGAGA AGAAAGACTT AGAGCACGAA GTAGAAGAAT TACATAGAAC CATCCAGAAA 
CATCAACAGC AAAAAGACTT CATTGATGGA AATGTTGAGA GTCTTATGAA TGAACTAAAA 
ATAGAAAAAT CACTCAAGCA CCATGAAGAT ATTGTAGATG AAATTGAGTG CATCGAGAAG 
ACTCTCCTGA AACGTCGAGC AGAGCTCAGG GAGGCTGACC GACTGCTGGC GGAAGCCGAG 
AGTGAACTTT CACGCACAAC AGAGAAAACA AAAAATGCTG TTGAAAAGTT CACTGATGCC 
AAGAGGAATT TGTTGCAAAC TGAGAAAGAT GCTGAGGAGT TAGAAAGGAG AGCTCAGGAA 
ACAGCTGTTA GCCTTGTCAA GGCTGATCAA CAGCTCAGAT TGTTACAGGC GGACACAAAG 
GATTTGGAGC AGCACAAAAC TGAGCAAGAA GAGATCTTGA AAGAGATAAA CAAAGTTGTT 
GCAGCAAAAG ACTCAGACTT CCAGTGTTTA AACACGAAGA AGGAGAAGCT AACAGAAGAG 
CTGCAGAAGC TGCAGAAAGA CATCGAGACG GCAGCGCGCA CGGAGGATCA CCACCTGCAG 
GTCCTGAGAG AGTCGGAGGC CCTCCTGCAG GCCAAGAGAG CGGAGCTGGA CAAGCTGAGG 
AGCCAGGTAA CGAGTCAGCA GCAGGAGATG GCTGTCTTGG ACAGACAGCT GGGGCATAAG 
AAGGAGGAGC TGCATCTACT CCAGGGAAGC ATGGTCCAGG CCAGAGCTGA CCTCCAGGAA 
GCGCTGAGGC TGGGAGAAAC TGAAGTAACT GAGAAGTGCA ACCACATCAG GGAACTAAAA 
GGTCTTCTGG AAGAACTGAG TTTTCAGAAA GGAGAACTGA ATGTCCAGAT TAGTGAGAAA 
AAAAATCAAC TTTCACTTAT AAAGCAAGAA ATTGAAAAAG AGGAAGAAAA TCTTCAGGTA 
GTTTTAGGGC AGATGTCTAA ACATAAAACT GAACTTAAGA ATATTCTGGA CATGTTGCAA 
CTTGAAAACA ATGAGCTGCA AGCTTTGAAG CTACAGCATG AGCAAAAGAT GTCTGAGTTA 
GAGAAGGCTC AGGTTGAAGT GCTGGAGGAG AAACTGGAGT TGGAGAATTT GCAGCAGACA 
GCCCTGCGAC AGAAAGGGGA GATCGAGTGG CAGAAGCAGC TCTTAGAGAG GGACAAGCGA 
GAGATTGAGC GGATAACTGC TGAGACCCGA GCATTGCAGT TGTGTGTTGA GTCTTTGTGC 
AAACAAAAGG AAGATCTTGA GGAGAAATGT GACAGCTGGG AGAAGAAGCT GGCACAGACC 
AAACGGGTTT TAGCAGCCAC AGAAGAGAAT AGCAAAGCAA AGCAATCAGA CTTAGAAAAG 
TTGGAATTGG ATATCAGAAA ACTGGAACAG GAACTAGACC AGCTAAACAG AAACAAACTC 
TCACTGCACA GAGACATCGC GGCCATGCAG CAGCAGGTCC AAGAAAAACG AGAAGCAGTA 
AACTCATTAC AGGAGGAACT AGCTGATGTC CAAGCCCATT TGAACCTAGC GAAACAGGAC 
CTGCTCCACA CCACCAAGCG CCAGGATGTC CTGCTTGGCG AGCAGACCAG GCTGCAGGAG 
GACCTCCAGG AACGCAGAAA GAAGTTGGAA GTCTGCCAAA AAGAAGAGGC AACAAAGCGG 
CAGCACCTGC AAGCGCTTCA GACGGAGATG GAGGCGAACG AGGCCGAGCT GGCCCGGCAG 
GAGACGGTAC CGCACCCGCG GGCACGCGCG GGGGGGCACT GCCACGCGCA GAGCGCTCTT 
CCCCTGGGGT CTGAGGTGGA GGCGAACGAG AGCGAGCTGG CCCGGCAGGA GACGACATTT 
CAGAGACTCC AGAAAGAGAG AGAAAGTGAA GAAAAAAAGT TAGAAGCCAG TCGAGCGACT 
CTGCAGGAGC AGCAGCAGCA GCTGGAAAAG GAAATCGCAG ACCAGAAGAA CGTGCTGGAC 
CAGGTGCTCA GCCAGGTGCT GGGGGCCAAG GAGCGCGCCA GGACTCTGCA GCAGGAGGAG 
AGGTGGGGTG AGACCCTGGA GAAGACGCTC TCCCAGACCC AGCAACAGCT CTCAGAACGG 
GAGCAGCAGT TGTTGGAAAA GTCAGCTGAG CTGCTGGCTC TTCAGAAAGA GGCAGACTCG 
ATGAGGGCAG ACTTCAGCCT CTTGCGAAAC CAGTTCTTGA CGGAGAGGAA GAAAGCCGAG 
AAGCAGGTAG CCAGCCTCAG GGAAGCGCTC AAGACCCAGC GCAGCCAGCT GGAGAAGACC 
CTTCTCGAGC AGAAGCAGGA GAACAGCTCC ATGCAGAAGG AGATGGCGAC TATCGAGCAG 
GTGGCGCAGG ACAACCACGA GCGGGCCCGG CGCCTGATGC GGGAGCTCAG CCAGATGCAG 
CAGGAGTACG CGGAGCTCAA GAAGCAGATG GTAAACCAGA AAGATCTGGA AAGAAGACAA 
GTAGAAATCA GTGAAGCGAT GAGGACACTT AAGTCTGAAG TGAAAGACGA GATCAGAGCC 
AGCCTGAAGA ACCTCAACCA GTTTCTCCCG GAGCTGCCAG CAGACCTGGA GGCCATTCTG 
GAAAGGAGCG AACACCTAGA CGGAGGGCTG GAGAGCTTGA AGGAGAACTT TCCATTCGCC 
CTGAGTGAGA AGCCATCACC TTTGGAAGAG AAACTACATT TTTCTCGCGT TCACATAATG 
GATGAGCACT GGCGTGGAGA AGCGCTCCGG GAGAAGCTGC GGCACCGTGA GGACCGACTC 
AAGGCCCAGC TCCGACACTG TATGTCCAAG CAGGCAGAGG TGTTAAGCAA AGGGAAGCGG 
CAGACCGAGG GCACGTTACA CAGCCTGAGG AGGCAGGTCG ACGCCTTAGG GGAGCTGGTC 
ACCAGCACGT CTGTGGAGTC CACGTCGTCA GCCACTCTGT CTCAGCTCGA ATCTTCCCTT 
GCAGAGGACT CCCATCTTGG ACCAAGTCAG AGCTCCTTCC ATCAAGTTCC ACAAGGTCCA 
TCACAATCAG TAGACAGTTA CCGACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025138131.1
CDS	359..7528
Translation	MKKGPQQKISKTKMPPSSHSPSPSSLTSNMRSRSLSPLNGSETLPFHSGRQWYGQVEIIGENSMLLDYHDQKEADSHAGVRYITEALVKKLTKQENLTLVKSLNLSLSKDGGKKFKYIENLEKCTKLEILNLSHNLIGKIEKVDKLLKLRELNLSYNKICKIEGIENMHNLQKLNLAGNEIEHIPAWLGKKLKCLRVLNLKANKISSLQDVSKLKPLQDLTSLILAENPVVTLPHYLQFTIFHLRSLESLEGQPVTTQDRQEAFERFSLEEVERLERDLEKKMMETEELKSKQAQFLEEIKNQDKLNRSLKEEAMLQKQSCEELESNLNTKNELPLSRFLADPCLPILYSSLKLRQKTMELTRACQKQYELEQELAFYKIDAKFEPLNYYPSEYVDVDKAPDESPYIGKSRYKRNMFATESYIVNNAQPVEIKRMEPEEGEQLRNEHVSLKAHMPLDTELEDKENKISTAQARLSALHDEIEKAEQQILRATEEFKQLEEAVQLKKISEAEKDFLLKQLSGRIQLLNKLRQEALDLEMQMEKQRGEIAEKQEEIKDLQRVIDSLDSKDPKHCHMKAQKRGKEQQFDIMNKQYKQLESRLDEILSRIAKETEEIKDLEQQLTEGQIAANEALKKDLEGVISGLQEYLGTVKGQAAQAQSECRRLQDEKEALLQRLAEVEQERDQLEAVALDAEAMRKELAELESALQEQHEVNASLQQTHGDLSAYEAELEAQLRMRDAEASQLKEELGELTRLSQLEQSTLQTELEKEKQALKNALGKAQLSEEKEQENSELRTQLKQLQDDNNLLKQQLKDFQNHLNQVADGLIRPEEVAARVDEVRRKLKSGAGEMRIHSPSDVLGKSLADLQIQFSEMLTRSQWEKEEAQGRERKLQEEMALHQEKLVDGQEAFRQACERALEARINFDKRQHEARIQQLENEIHYLQENLKSMEEIQGLTDLQLQEADEEKERILAQLQELEKKKKLEDAKSQAQVLGLDKELKKLKKAVATSDKIATAELTIAKDQLKSLHGTVMKINQERAEDLQEAERFSRKAAQAARDLTRAEAEIELLQNLLREREEQMEKVEAGTRGASSQALEIERWNETMERQRSEIARLRDSLDRTGADNRGGIENILEEIAELRREVSYQNDYISSMADPFKRRGYWYFMPPPSSSKVSSHSSQATKDSGVGMKYAASTPIRKPQQNGREDGAPPAASGYWVYSPIRSGLHKSFSNKDADSGGESREESELDDHDDRPFVPPPGYMMYTVFPDGSPVPQGMALYAPPPPLPNNSRPLSPGTVVYGPPPAGAPIVYGPPPPSFSVPLIPVGVLHCNVPEHHALENEVSRLEDIIQHLKSKKQEQRMRVCKRQSEKEIEELHHNIDELLQEKKDLEHEVEELHRTIQKHQQQKDFIDGNVESLMNELKIEKSLKHHEDIVDEIECIEKTLLKRRAELREADRLLAEAESELSRTTEKTKNAVEKFTDAKRNLLQTEKDAEELERRAQETAVSLVKADQQLRLLQADTKDLEQHKTEQEEILKEINKVVAAKDSDFQCLNTKKEKLTEELQKLQKDIETAARTEDHHLQVLRESEALLQAKRAELDKLRSQVTSQQQEMAVLDRQLGHKKEELHLLQGSMVQARADLQEALRLGETEVTEKCNHIRELKGLLEELSFQKGELNVQISEKKNQLSLIKQEIEKEEENLQVVLGQMSKHKTELKNILDMLQLENNELQALKLQHEQKMSELEKAQVEVLEEKLELENLQQTALRQKGEIEWQKQLLERDKREIERITAETRALQLCVESLCKQKEDLEEKCDSWEKKLAQTKRVLAATEENSKAKQSDLEKLELDIRKLEQELDQLNRNKLSLHRDIAAMQQQVQEKREAVNSLQEELADVQAHLNLAKQDLLHTTKRQDVLLGEQTRLQEDLQERRKKLEVCQKEEATKRQHLQALQTEMEANEAELARQETVPHPRARAGGHCHAQSALPLGSEVEANESELARQETTFQRLQKERESEEKKLEASRATLQEQQQQLEKEIADQKNVLDQVLSQVLGAKERARTLQQEERWGETLEKTLSQTQQQLSEREQQLLEKSAELLALQKEADSMRADFSLLRNQFLTERKKAEKQVASLREALKTQRSQLEKTLLEQKQENSSMQKEMATIEQVAQDNHERARRLMRELSQMQQEYAELKKQMVNQKDLERRQVEISEAMRTLKSEVKDEIRASLKNLNQFLPELPADLEAILERSEHLDGGLESLKENFPFALSEKPSPLEEKLHFSRVHIMDEHWRGEALREKLRHREDRLKAQLRHCMSKQAEVLSKGKRQTEGTLHSLRRQVDALGELVTSTSVESTSSATLSQLESSLAEDSHLGPSQSSFHQVPQGPSQSVDSYRH

Target ORF information:

RefSeq Version	XM_025282346.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centriolin (CNTRL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025282346.1

ORF Insert Sequence:

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCCCTTGTC TCGGTTTCTT 
GCGGACCCTT GTCTGCCGAT TCTTTATTCT TCCTTAAAGC TAAGACAGAA GACCATGGAA 
CTAACGCGAG CATGTCAAAA ACAGTATGAA CTGGAACAGG AGTTGGCTTT TTACAAAATC 
GATGCTAAAT TTGAGCCACT GAATTATTAT CCCTCAGAGT ATGTTGACGT TGATAAAGCC 
CCAGATGAAA GCCCTTACAT TGGCAAGTCC AGATACAAGA GAAATATGTT CGCCACAGAG 
AGTTACATTG TGAATAATGC CCAGCCAGTT GAGATCAAGA GGATGGAGCC AGAGGAAGGG 
GAGCAACTTA GAAACGAGCA TGTGAGCTTG AAAGCCCATA TGCCACTGGA CACGGAGCTG 
GAAGACAAAG AGAACAAAAT AAGTACAGCA CAAGCTCGAC TATCAGCACT GCATGATGAA 
ATAGAAAAGG CAGAACAACA AATTTTAAGA GCCACTGAAG AATTTAAACA ACTGGAAGAA 
GCTGTACAAC TTAAAAAGAT TTCAGAAGCA GAGAAAGACT TTCTTCTCAA GCAGCTGAGT 
GGCAGGATAC AGCTTCTAAA TAAATTACGC CAGGAAGCTC TTGATCTAGA AATGCAGATG 
GAAAAGCAAA GAGGAGAAAT TGCTGAAAAG CAGGAGGAAA TCAAGGACCT CCAAAGAGTT 
ATTGATAGTC TGGATTCCAA AGACCCAAAA CATTGCCATA TGAAGGCTCA GAAAAGGGGT 
AAAGAACAAC AATTTGACAT TATGAACAAG CAGTACAAAC AACTTGAGAG CCGTTTGGAC 
GAGATACTTT CTAGAATTGC TAAAGAAACT GAAGAGATTA AGGACCTTGA ACAACAGCTT 
ACTGAAGGCC AGATAGCAGC AAATGAAGCC CTGAAGAAGG ACTTAGAAGG CGTCATCAGT 
GGCCTGCAAG AATACCTGGG CACTGTGAAG GGCCAGGCCG CTCAGGCCCA GAGCGAGTGC 
AGAAGGCTGC AGGATGAGAA AGAGGCGCTG CTGCAGAGGT TGGCCGAGGT CGAGCAGGAA 
AGGGACCAGC TGGAAGCAGT CGCCTTAGAT GCAGAAGCTA TGAGGAAGGA GCTTGCAGAG 
CTAGAAAGCG CCCTCCAAGA GCAGCATGAG GTGAACGCGT CCCTGCAGCA GACCCACGGA 
GACCTCAGTG CCTACGAGGC TGAGCTAGAG GCACAGCTGA GGATGAGGGA TGCCGAAGCC 
AGCCAGCTCA AAGAAGAGCT GGGAGAACTA ACAAGGCTCA GCCAGCTAGA ACAGTCAACT 
CTACAAACAG AACTTGAGAA AGAAAAACAA GCCCTCAAGA ACGCCCTGGG AAAAGCCCAG 
CTCTCGGAAG AAAAAGAACA AGAAAACAGT GAGCTCCGCA CCCAGCTTAA ACAGCTACAG 
GATGACAATA ACCTGTTAAA ACAGCAACTT AAAGATTTCC AGAATCACCT TAATCAGGTA 
GCTGATGGTC TGATTCGTCC AGAAGAAGTG GCAGCTCGTG TAGATGAAGT AAGGAGAAAA 
TTGAAATCAG GCGCTGGAGA GATGAGAATC CACAGTCCTT CAGATGTCTT AGGGAAAAGT 
CTTGCTGACT TGCAGATACA ATTCAGTGAA ATGCTGACAC GTTCTCAGTG GGAGAAAGAG 
GAAGCCCAAG GGAGGGAAAG AAAACTCCAA GAAGAAATGG CTCTGCATCA GGAGAAGCTG 
GTGGATGGAC AGGAAGCGTT CAGGCAGGCC TGTGAGAGAG CGCTAGAAGC AAGGATTAAT 
TTTGACAAGA GGCAACATGA AGCAAGAATT CAGCAGTTAG AGAATGAGAT TCACTATTTA 
CAAGAAAATC TAAAAAGTAT GGAGGAAATC CAAGGGCTTA CAGATCTGCA GCTGCAGGAA 
GCTGATGAAG AGAAGGAGAG AATTCTAGCC CAACTCCAGG AGTTAGAGAA GAAGAAGAAA 
CTTGAAGATG CCAAATCTCA GGCACAGGTT CTAGGTTTAG ATAAAGAATT GAAGAAACTA 
AAGAAGGCTG TGGCCACCTC TGATAAGATA GCCACAGCTG AGCTCACCAT CGCCAAAGAC 
CAGCTCAAGT CCCTTCATGG AACTGTTATG AAAATTAACC AGGAGCGTGC AGAGGACCTG 
CAGGAAGCAG AGCGGTTCAG CAGAAAGGCA GCACAGGCCG CGAGGGATCT GACCCGAGCG 
GAAGCGGAGA TCGAACTCCT GCAGAACCTC CTCAGGGAGA GAGAGGAGCA GATGGAGAAG 
GTAGAGGCAG GCACCCGAGG AGCAAGCTCA CAGGCGCTGG AAATCGAGAG GTGGAATGAG 
ACCATGGAGA GACAGAGGAG CGAGATCGCA AGGCTACGGG ACTCACTAGA CCGCACCGGG 
GCGGATAACA GAGGAGGCAT TGAAAATATT TTGGAAGAAA TAGCTGAACT CCGACGTGAA 
GTTTCTTACC AGAATGATTA CATCAGCAGC ATGGCAGATC CTTTCAAAAG ACGAGGCTAT 
TGGTACTTTA TGCCACCACC ATCATCATCA AAAGTTTCCA GCCACAGCTC CCAGGCCACA 
AAGGACTCTG GTGTCGGGAT GAAGTACGCA GCCTCGACTC CTATTAGAAA ACCACAGCAG 
AACGGGAGGG AAGACGGTGC CCCTCCAGCT GCCTCCGGAT ACTGGGTGTA TTCCCCCATC 
AGGAGCGGCT TACATAAATC GTTTTCAAAT AAAGATGCAG ACAGTGGAGG AGAGAGCCGG 
GAGGAGAGCG AGCTGGATGA CCACGATGAC CGCCCGTTCG TCCCTCCTCC TGGATACATG 
ATGTACACCG TGTTCCCCGA CGGCTCTCCT GTACCACAGG GCATGGCCCT CTACGCACCG 
CCGCCTCCCT TGCCCAACAA TAGCCGACCT CTCAGCCCTG GCACTGTTGT GTATGGCCCG 
CCCCCTGCTG GTGCCCCCAT TGTCTACGGG CCCCCACCGC CCAGCTTCTC CGTCCCCCTC 
ATCCCAGTGG GCGTGCTGCA CTGCAACGTC CCGGAGCACC ATGCCTTAGA GAATGAGGTT 
TCTAGATTGG AAGACATAAT ACAGCATTTA AAATCAAAGA AGCAGGAACA GAGGATGAGA 
GTATGCAAGC GGCAGTCTGA GAAAGAAATA GAAGAACTGC ATCATAATAT TGATGAACTT 
TTGCAAGAGA AGAAAGACTT AGAGCACGAA GTAGAAGAAT TACATAGAAC CATCCAGAAA 
CATCAACAGC AAAAAGACTT CATTGATGGA AATGTTGAGA GTCTTATGAA TGAACTAAAA 
ATAGAAAAAT CACTCAAGCA CCATGAAGAT ATTGTAGATG AAATTGAGTG CATCGAGAAG 
ACTCTCCTGA AACGTCGAGC AGAGCTCAGG GAGGCTGACC GACTGCTGGC GGAAGCCGAG 
AGTGAACTTT CACGCACAAC AGAGAAAACA AAAAATGCTG TTGAAAAGTT CACTGATGCC 
AAGAGGAATT TGTTGCAAAC TGAGAAAGAT GCTGAGGAGT TAGAAAGGAG AGCTCAGGAA 
ACAGCTGTTA GCCTTGTCAA GGCTGATCAA CAGCTCAGAT TGTTACAGGC GGACACAAAG 
GATTTGGAGC AGCACAAAAC TGAGCAAGAA GAGATCTTGA AAGAGATAAA CAAAGTTGTT 
GCAGCAAAAG ACTCAGACTT CCAGTGTTTA AACACGAAGA AGGAGAAGCT AACAGAAGAG 
CTGCAGAAGC TGCAGAAAGA CATCGAGACG GCAGCGCGCA CGGAGGATCA CCACCTGCAG 
GTCCTGAGAG AGTCGGAGGC CCTCCTGCAG GCCAAGAGAG CGGAGCTGGA CAAGCTGAGG 
AGCCAGGTAA CGAGTCAGCA GCAGGAGATG GCTGTCTTGG ACAGACAGCT GGGGCATAAG 
AAGGAGGAGC TGCATCTACT CCAGGGAAGC ATGGTCCAGG CCAGAGCTGA CCTCCAGGAA 
GCGCTGAGGC TGGGAGAAAC TGAAGTAACT GAGAAGTGCA ACCACATCAG GGAACTAAAA 
GGTCTTCTGG AAGAACTGAG TTTTCAGAAA GGAGAACTGA ATGTCCAGAT TAGTGAGAAA 
AAAAATCAAC TTTCACTTAT AAAGCAAGAA ATTGAAAAAG AGGAAGAAAA TCTTCAGGTA 
GTTTTAGGGC AGATGTCTAA ACATAAAACT GAACTTAAGA ATATTCTGGA CATGTTGCAA 
CTTGAAAACA ATGAGCTGCA AGCTTTGAAG CTACAGCATG AGCAAAAGAT GTCTGAGTTA 
GAGAAGGCTC AGGTTGAAGT GCTGGAGGAG AAACTGGAGT TGGAGAATTT GCAGCAGACA 
GCCCTGCGAC AGAAAGGGGA GATCGAGTGG CAGAAGCAGC TCTTAGAGAG GGACAAGCGA 
GAGATTGAGC GGATAACTGC TGAGACCCGA GCATTGCAGT TGTGTGTTGA GTCTTTGTGC 
AAACAAAAGG AAGATCTTGA GGAGAAATGT GACAGCTGGG AGAAGAAGCT GGCACAGACC 
AAACGGGTTT TAGCAGCCAC AGAAGAGAAT AGCAAAGCAA AGCAATCAGA CTTAGAAAAG 
TTGGAATTGG ATATCAGAAA ACTGGAACAG GAACTAGACC AGCTAAACAG AAACAAACTC 
TCACTGCACA GAGACATCGC GGCCATGCAG CAGCAGGTCC AAGAAAAACG AGAAGCAGTA 
AACTCATTAC AGGAGGAACT AGCTGATGTC CAAGCCCATT TGAACCTAGC GAAACAGGAC 
CTGCTCCACA CCACCAAGCG CCAGGATGTC CTGCTTGGCG AGCAGACCAG GCTGCAGGAG 
GACCTCCAGG AACGCAGAAA GAAGTTGGAA GTCTGCCAAA AAGAAGAGGC AACAAAGCGG 
CAGCACCTGC AAGCGCTTCA GACGGAGATG GAGGCGAACG AGGCCGAGCT GGCCCGGCAG 
GAGACGGTAC CGCACCCGCG GGCACGCGCG GGGGGGCACT GCCACGCGCA GAGCGCTCTT 
CCCCTGGGGT CTGAGGTGGA GGCGAACGAG AGCGAGCTGG CCCGGCAGGA GACGACATTT 
CAGAGACTCC AGAAAGAGAG AGAAAGTGAA GAAAAAAAGT TAGAAGCCAG TCGAGCGACT 
CTGCAGGAGC AGCAGCAGCA GCTGGAAAAG GAAATCGCAG ACCAGAAGAA CGTGCTGGAC 
CAGGTGCTCA GCCAGGTGCT GGGGGCCAAG GAGCGCGCCA GGACTCTGCA GCAGGAGGAG 
AGGTGGGGTG AGACCCTGGA GAAGACGCTC TCCCAGACCC AGCAACAGCT CTCAGAACGG 
GAGCAGCAGT TGTTGGAAAA GTCAGCTGAG CTGCTGGCTC TTCAGAAAGA GGCAGACTCG 
ATGAGGGCAG ACTTCAGCCT CTTGCGAAAC CAGTTCTTGA CGGAGAGGAA GAAAGCCGAG 
AAGCAGGTAG CCAGCCTCAG GGAAGCGCTC AAGACCCAGC GCAGCCAGCT GGAGAAGACC 
CTTCTCGAGC AGAAGCAGGA GAACAGCTCC ATGCAGAAGG AGATGGCGAC TATCGAGCAG 
GTGGCGCAGG ACAACCACGA GCGGGCCCGG CGCCTGATGC GGGAGCTCAG CCAGATGCAG 
CAGGAGTACG CGGAGCTCAA GAAGCAGATG GTAAACCAGA AAGATCTGGA AAGAAGACAA 
GTAGAAATCA GTGAAGCGAT GAGGACACTT AAGTCTGAAG TGAAAGACGA GATCAGAGCC 
AGCCTGAAGA ACCTCAACCA GTTTCTCCCG GAGCTGCCAG CAGACCTGGA GGCCATTCTG 
GAAAGGAGCG AACACCTAGA CGGAGGGCTG GAGAGCTTGA AGGAGAACTT TCCATTCGCC 
CTGAGTGAGA AGCCATCACC TTTGGAAGAG AAACTACATT TTTCTCGCGT TCACATAATG 
GATGAGCACT GGCGTGGAGA AGCGCTCCGG GAGAAGCTGC GGCACCGTGA GGACCGACTC 
AAGGCCCAGC TCCGACACTG TATGTCCAAG CAGGCAGAGG TGTTAAGCAA AGGGAAGCGG 
CAGACCGAGG GCACGTTACA CAGCCTGAGG AGGCAGGTCG ACGCCTTAGG GGAGCTGGTC 
ACCAGCACGT CTGTGGAGTC CACGTCGTCA GCCACTCTGT CTCAGCTCGA ATCTTCCCTT 
GCAGAGGACT CCCATCTTGG ACCAAGTCAG AGCTCCTTCC ATCAAGTTCC ACAAGGTCCA 
TCACAATCAG TAGACAGTTA CCGACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTRL ( XM_025282347.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025138132.1 Bubalus bubalis centriolin (CNTRL), transcript variant X5, mRNA.

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CloneID	OBu63435
Clone ID Related Accession (Same CDS sequence)	XM_025282347.1
Accession Version	XM_025282347.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	centriolin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCTAAGACA GAAGACCATG 
GAACTAACGC GAGCATGTCA AAAACAGTAT GAACTGGAAC AGGAGTTGGC TTTTTACAAA 
ATCGATGCTA AATTTGAGCC ACTGAATTAT TATCCCTCAG AGTATGTTGA CGTTGATAAA 
GCCCCAGATG AAAGCCCTTA CATTGGCAAG TCCAGATACA AGAGAAATAT GTTCGCCACA 
GAGAGTTACA TTGTGAATAA TGCCCAGCCA GTTGAGATCA AGAGGATGGA GCCAGAGGAA 
GGGGAGCAAC TTAGAAACGA GCATGTGAGC TTGAAAGCCC ATATGCCACT GGACACGGAG 
CTGGAAGACA AAGAGAACAA AATAAGTACA GCACAAGCTC GACTATCAGC ACTGCATGAT 
GAAATAGAAA AGGCAGAACA ACAAATTTTA AGAGCCACTG AAGAATTTAA ACAACTGGAA 
GAAGCTGTAC AACTTAAAAA GATTTCAGAA GCAGAGAAAG ACTTTCTTCT CAAGCAGCTG 
AGTGGCAGGA TACAGCTTCT AAATAAATTA CGCCAGGAAG CTCTTGATCT AGAAATGCAG 
ATGGAAAAGC AAAGAGGAGA AATTGCTGAA AAGCAGGAGG AAATCAAGGA CCTCCAAAGA 
GTTATTGATA GTCTGGATTC CAAAGACCCA AAACATTGCC ATATGAAGGC TCAGAAAAGG 
GGTAAAGAAC AACAATTTGA CATTATGAAC AAGCAGTACA AACAACTTGA GAGCCGTTTG 
GACGAGATAC TTTCTAGAAT TGCTAAAGAA ACTGAAGAGA TTAAGGACCT TGAACAACAG 
CTTACTGAAG GCCAGATAGC AGCAAATGAA GCCCTGAAGA AGGACTTAGA AGGCGTCATC 
AGTGGCCTGC AAGAATACCT GGGCACTGTG AAGGGCCAGG CCGCTCAGGC CCAGAGCGAG 
TGCAGAAGGC TGCAGGATGA GAAAGAGGCG CTGCTGCAGA GGTTGGCCGA GGTCGAGCAG 
GAAAGGGACC AGCTGGAAGC AGTCGCCTTA GATGCAGAAG CTATGAGGAA GGAGCTTGCA 
GAGCTAGAAA GCGCCCTCCA AGAGCAGCAT GAGGTGAACG CGTCCCTGCA GCAGACCCAC 
GGAGACCTCA GTGCCTACGA GGCTGAGCTA GAGGCACAGC TGAGGATGAG GGATGCCGAA 
GCCAGCCAGC TCAAAGAAGA GCTGGGAGAA CTAACAAGGC TCAGCCAGCT AGAACAGTCA 
ACTCTACAAA CAGAACTTGA GAAAGAAAAA CAAGCCCTCA AGAACGCCCT GGGAAAAGCC 
CAGCTCTCGG AAGAAAAAGA ACAAGAAAAC AGTGAGCTCC GCACCCAGCT TAAACAGCTA 
CAGGATGACA ATAACCTGTT AAAACAGCAA CTTAAAGATT TCCAGAATCA CCTTAATCAG 
GTAGCTGATG GTCTGATTCG TCCAGAAGAA GTGGCAGCTC GTGTAGATGA AGTAAGGAGA 
AAATTGAAAT CAGGCGCTGG AGAGATGAGC AGAATCCACA GTCCTTCAGA TGTCTTAGGG 
AAAAGTCTTG CTGACTTGCA GATACAATTC AGTGAAATGC TGACACGTTC TCAGTGGGAG 
AAAGAGGAAG CCCAAGGGAG GGAAAGAAAA CTCCAAGAAG AAATGGCTCT GCATCAGGAG 
AAGCTGGTGG ATGGACAGGA AGCGTTCAGG CAGGCCTGTG AGAGAGCGCT AGAAGCAAGG 
ATTAATTTTG ACAAGAGGCA ACATGAAGCA AGAATTCAGC AGTTAGAGAA TGAGATTCAC 
TATTTACAAG AAAATCTAAA AAGTATGGAG GAAATCCAAG GGCTTACAGA TCTGCAGCTG 
CAGGAAGCTG ATGAAGAGAA GGAGAGAATT CTAGCCCAAC TCCAGGAGTT AGAGAAGAAG 
AAGAAACTTG AAGATGCCAA ATCTCAGGCA CAGGTTCTAG GTTTAGATAA AGAATTGAAG 
AAACTAAAGA AGGCTGTGGC CACCTCTGAT AAGATAGCCA CAGCTGAGCT CACCATCGCC 
AAAGACCAGC TCAAGTCCCT TCATGGAACT GTTATGAAAA TTAACCAGGA GCGTGCAGAG 
GACCTGCAGG AAGCAGAGCG GTTCAGCAGA AAGGCAGCAC AGGCCGCGAG GGATCTGACC 
CGAGCGGAAG CGGAGATCGA ACTCCTGCAG AACCTCCTCA GGGAGAGAGA GGAGCAGATG 
GAGAAGGTAG AGGCAGGCAC CCGAGGAGCA AGCTCACAGG CGCTGGAAAT CGAGAGGTGG 
AATGAGACCA TGGAGAGACA GAGGAGCGAG ATCGCAAGGC TACGGGACTC ACTAGACCGC 
ACCGGGGCGG ATAACAGAGG AGGCATTGAA AATATTTTGG AAGAAATAGC TGAACTCCGA 
CGTGAAGTTT CTTACCAGAA TGATTACATC AGCAGCATGG CAGATCCTTT CAAAAGACGA 
GGCTATTGGT ACTTTATGCC ACCACCATCA TCATCAAAAG TTTCCAGCCA CAGCTCCCAG 
GCCACAAAGG ACTCTGGTGT CGGGATGAAG TACGCAGCCT CGACTCCTAT TAGAAAACCA 
CAGCAGAACG GGAGGGAAGA CGGTGCCCCT CCAGCTGCCT CCGGATACTG GGTGTATTCC 
CCCATCAGGA GCGGCTTACA TAAATCGTTT TCAAATAAAG ATGCAGACAG TGGAGGAGAG 
AGCCGGGAGG AGAGCGAGCT GGATGACCAC GATGACCGCC CGTTCGTCCC TCCTCCTGGA 
TACATGATGT ACACCGTGTT CCCCGACGGC TCTCCTGTAC CACAGGGCAT GGCCCTCTAC 
GCACCGCCGC CTCCCTTGCC CAACAATAGC CGACCTCTCA GCCCTGGCAC TGTTGTGTAT 
GGCCCGCCCC CTGCTGGTGC CCCCATTGTC TACGGGCCCC CACCGCCCAG CTTCTCCGTC 
CCCCTCATCC CAGTGGGCGT GCTGCACTGC AACGTCCCGG AGCACCATGC CTTAGAGAAT 
GAGGTTTCTA GATTGGAAGA CATAATACAG CATTTAAAAT CAAAGAAGCA GGAACAGAGG 
ATGAGAGTAT GCAAGCGGCA GTCTGAGAAA GAAATAGAAG AACTGCATCA TAATATTGAT 
GAACTTTTGC AAGAGAAGAA AGACTTAGAG CACGAAGTAG AAGAATTACA TAGAACCATC 
CAGAAACATC AACAGCAAAA AGACTTCATT GATGGAAATG TTGAGAGTCT TATGAATGAA 
CTAAAAATAG AAAAATCACT CAAGCACCAT GAAGATATTG TAGATGAAAT TGAGTGCATC 
GAGAAGACTC TCCTGAAACG TCGAGCAGAG CTCAGGGAGG CTGACCGACT GCTGGCGGAA 
GCCGAGAGTG AACTTTCACG CACAACAGAG AAAACAAAAA ATGCTGTTGA AAAGTTCACT 
GATGCCAAGA GGAATTTGTT GCAAACTGAG AAAGATGCTG AGGAGTTAGA AAGGAGAGCT 
CAGGAAACAG CTGTTAGCCT TGTCAAGGCT GATCAACAGC TCAGATTGTT ACAGGCGGAC 
ACAAAGGATT TGGAGCAGCA CAAAACTGAG CAAGAAGAGA TCTTGAAAGA GATAAACAAA 
GTTGTTGCAG CAAAAGACTC AGACTTCCAG TGTTTAAACA CGAAGAAGGA GAAGCTAACA 
GAAGAGCTGC AGAAGCTGCA GAAAGACATC GAGACGGCAG CGCGCACGGA GGATCACCAC 
CTGCAGGTCC TGAGAGAGTC GGAGGCCCTC CTGCAGGCCA AGAGAGCGGA GCTGGACAAG 
CTGAGGAGCC AGGTAACGAG TCAGCAGCAG GAGATGGCTG TCTTGGACAG ACAGCTGGGG 
CATAAGAAGG AGGAGCTGCA TCTACTCCAG GGAAGCATGG TCCAGGCCAG AGCTGACCTC 
CAGGAAGCGC TGAGGCTGGG AGAAACTGAA GTAACTGAGA AGTGCAACCA CATCAGGGAA 
CTAAAAGGTC TTCTGGAAGA ACTGAGTTTT CAGAAAGGAG AACTGAATGT CCAGATTAGT 
GAGAAAAAAA ATCAACTTTC ACTTATAAAG CAAGAAATTG AAAAAGAGGA AGAAAATCTT 
CAGGTAGTTT TAGGGCAGAT GTCTAAACAT AAAACTGAAC TTAAGAATAT TCTGGACATG 
TTGCAACTTG AAAACAATGA GCTGCAAGCT TTGAAGCTAC AGCATGAGCA AAAGATGTCT 
GAGTTAGAGA AGGCTCAGGT TGAAGTGCTG GAGGAGAAAC TGGAGTTGGA GAATTTGCAG 
CAGACAGCCC TGCGACAGAA AGGGGAGATC GAGTGGCAGA AGCAGCTCTT AGAGAGGGAC 
AAGCGAGAGA TTGAGCGGAT AACTGCTGAG ACCCGAGCAT TGCAGTTGTG TGTTGAGTCT 
TTGTGCAAAC AAAAGGAAGA TCTTGAGGAG AAATGTGACA GCTGGGAGAA GAAGCTGGCA 
CAGACCAAAC GGGTTTTAGC AGCCACAGAA GAGAATAGCA AAGCAAAGCA ATCAGACTTA 
GAAAAGTTGG AATTGGATAT CAGAAAACTG GAACAGGAAC TAGACCAGCT AAACAGAAAC 
AAACTCTCAC TGCACAGAGA CATCGCGGCC ATGCAGCAGC AGGTCCAAGA AAAACGAGAA 
GCAGTAAACT CATTACAGGA GGAACTAGCT GATGTCCAAG CCCATTTGAA CCTAGCGAAA 
CAGGACCTGC TCCACACCAC CAAGCGCCAG GATGTCCTGC TTGGCGAGCA GACCAGGCTG 
CAGGAGGACC TCCAGGAACG CAGAAAGAAG TTGGAAGTCT GCCAAAAAGA AGAGGCAACA 
AAGCGGCAGC ACCTGCAAGC GCTTCAGACG GAGATGGAGG CGAACGAGGC CGAGCTGGCC 
CGGCAGGAGA CGGTACCGCA CCCGCGGGCA CGCGCGGGGG GGCACTGCCA CGCGCAGAGC 
GCTCTTCCCC TGGGGTCTGA GGTGGAGGCG AACGAGAGCG AGCTGGCCCG GCAGGAGACG 
ACATTTCAGA GACTCCAGAA AGAGAGAGAA AGTGAAGAAA AAAAGTTAGA AGCCAGTCGA 
GCGACTCTGC AGGAGCAGCA GCAGCAGCTG GAAAAGGAAA TCGCAGACCA GAAGAACGTG 
CTGGACCAGG TGCTCAGCCA GGTGCTGGGG GCCAAGGAGC GCGCCAGGAC TCTGCAGCAG 
GAGGAGAGGT GGGGTGAGAC CCTGGAGAAG ACGCTCTCCC AGACCCAGCA ACAGCTCTCA 
GAACGGGAGC AGCAGTTGTT GGAAAAGTCA GCTGAGCTGC TGGCTCTTCA GAAAGAGGCA 
GACTCGATGA GGGCAGACTT CAGCCTCTTG CGAAACCAGT TCTTGACGGA GAGGAAGAAA 
GCCGAGAAGC AGGTAGCCAG CCTCAGGGAA GCGCTCAAGA CCCAGCGCAG CCAGCTGGAG 
AAGACCCTTC TCGAGCAGAA GCAGGAGAAC AGCTCCATGC AGAAGGAGAT GGCGACTATC 
GAGCAGGTGG CGCAGGACAA CCACGAGCGG GCCCGGCGCC TGATGCGGGA GCTCAGCCAG 
ATGCAGCAGG AGTACGCGGA GCTCAAGAAG CAGATGGTAA ACCAGAAAGA TCTGGAAAGA 
AGACAAGTAG AAATCAGTGA AGCGATGAGG ACACTTAAGT CTGAAGTGAA AGACGAGATC 
AGAGCCAGCC TGAAGAACCT CAACCAGTTT CTCCCGGAGC TGCCAGCAGA CCTGGAGGCC 
ATTCTGGAAA GGAGCGAACA CCTAGACGGA GGGCTGGAGA GCTTGAAGGA GAACTTTCCA 
TTCGCCCTGA GTGAGAAGCC ATCACCTTTG GAAGAGAAAC TACATTTTTC TCGCGTTCAC 
ATAATGGATG AGCACTGGCG TGGAGAAGCG CTCCGGGAGA AGCTGCGGCA CCGTGAGGAC 
CGACTCAAGG CCCAGCTCCG ACACTGTATG TCCAAGCAGG CAGAGGTGTT AAGCAAAGGG 
AAGCGGCAGA CCGAGGGCAC GTTACACAGC CTGAGGAGGC AGGTCGACGC CTTAGGGGAG 
CTGGTCACCA GCACGTCTGT GGAGTCCACG TCGTCAGCCA CTCTGTCTCA GCTCGAATCT 
TCCCTTGCAG AGGACTCCCA TCTTGGACCA AGTCAGAGCT CCTTCCATCA AGTTCCACAA 
GGTCCATCAC AATCAGTAGA CAGTTACCGA CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025138132.1
CDS	359..7474
Translation	MKKGPQQKISKTKMPPSSHSPSPSSLTSNMRSRSLSPLNGSETLPFHSGRQWYGQVEIIGENSMLLDYHDQKEADSHAGVRYITEALVKKLTKQENLTLVKSLNLSLSKDGGKKFKYIENLEKCTKLEILNLSHNLIGKIEKVDKLLKLRELNLSYNKICKIEGIENMHNLQKLNLAGNEIEHIPAWLGKKLKCLRVLNLKANKISSLQDVSKLKPLQDLTSLILAENPVVTLPHYLQFTIFHLRSLESLEGQPVTTQDRQEAFERFSLEEVERLERDLEKKMMETEELKSKQAQFLEEIKNQDKLNRSLKEEAMLQKQSCEELESNLNTKNELLRQKTMELTRACQKQYELEQELAFYKIDAKFEPLNYYPSEYVDVDKAPDESPYIGKSRYKRNMFATESYIVNNAQPVEIKRMEPEEGEQLRNEHVSLKAHMPLDTELEDKENKISTAQARLSALHDEIEKAEQQILRATEEFKQLEEAVQLKKISEAEKDFLLKQLSGRIQLLNKLRQEALDLEMQMEKQRGEIAEKQEEIKDLQRVIDSLDSKDPKHCHMKAQKRGKEQQFDIMNKQYKQLESRLDEILSRIAKETEEIKDLEQQLTEGQIAANEALKKDLEGVISGLQEYLGTVKGQAAQAQSECRRLQDEKEALLQRLAEVEQERDQLEAVALDAEAMRKELAELESALQEQHEVNASLQQTHGDLSAYEAELEAQLRMRDAEASQLKEELGELTRLSQLEQSTLQTELEKEKQALKNALGKAQLSEEKEQENSELRTQLKQLQDDNNLLKQQLKDFQNHLNQVADGLIRPEEVAARVDEVRRKLKSGAGEMSRIHSPSDVLGKSLADLQIQFSEMLTRSQWEKEEAQGRERKLQEEMALHQEKLVDGQEAFRQACERALEARINFDKRQHEARIQQLENEIHYLQENLKSMEEIQGLTDLQLQEADEEKERILAQLQELEKKKKLEDAKSQAQVLGLDKELKKLKKAVATSDKIATAELTIAKDQLKSLHGTVMKINQERAEDLQEAERFSRKAAQAARDLTRAEAEIELLQNLLREREEQMEKVEAGTRGASSQALEIERWNETMERQRSEIARLRDSLDRTGADNRGGIENILEEIAELRREVSYQNDYISSMADPFKRRGYWYFMPPPSSSKVSSHSSQATKDSGVGMKYAASTPIRKPQQNGREDGAPPAASGYWVYSPIRSGLHKSFSNKDADSGGESREESELDDHDDRPFVPPPGYMMYTVFPDGSPVPQGMALYAPPPPLPNNSRPLSPGTVVYGPPPAGAPIVYGPPPPSFSVPLIPVGVLHCNVPEHHALENEVSRLEDIIQHLKSKKQEQRMRVCKRQSEKEIEELHHNIDELLQEKKDLEHEVEELHRTIQKHQQQKDFIDGNVESLMNELKIEKSLKHHEDIVDEIECIEKTLLKRRAELREADRLLAEAESELSRTTEKTKNAVEKFTDAKRNLLQTEKDAEELERRAQETAVSLVKADQQLRLLQADTKDLEQHKTEQEEILKEINKVVAAKDSDFQCLNTKKEKLTEELQKLQKDIETAARTEDHHLQVLRESEALLQAKRAELDKLRSQVTSQQQEMAVLDRQLGHKKEELHLLQGSMVQARADLQEALRLGETEVTEKCNHIRELKGLLEELSFQKGELNVQISEKKNQLSLIKQEIEKEEENLQVVLGQMSKHKTELKNILDMLQLENNELQALKLQHEQKMSELEKAQVEVLEEKLELENLQQTALRQKGEIEWQKQLLERDKREIERITAETRALQLCVESLCKQKEDLEEKCDSWEKKLAQTKRVLAATEENSKAKQSDLEKLELDIRKLEQELDQLNRNKLSLHRDIAAMQQQVQEKREAVNSLQEELADVQAHLNLAKQDLLHTTKRQDVLLGEQTRLQEDLQERRKKLEVCQKEEATKRQHLQALQTEMEANEAELARQETVPHPRARAGGHCHAQSALPLGSEVEANESELARQETTFQRLQKERESEEKKLEASRATLQEQQQQLEKEIADQKNVLDQVLSQVLGAKERARTLQQEERWGETLEKTLSQTQQQLSEREQQLLEKSAELLALQKEADSMRADFSLLRNQFLTERKKAEKQVASLREALKTQRSQLEKTLLEQKQENSSMQKEMATIEQVAQDNHERARRLMRELSQMQQEYAELKKQMVNQKDLERRQVEISEAMRTLKSEVKDEIRASLKNLNQFLPELPADLEAILERSEHLDGGLESLKENFPFALSEKPSPLEEKLHFSRVHIMDEHWRGEALREKLRHREDRLKAQLRHCMSKQAEVLSKGKRQTEGTLHSLRRQVDALGELVTSTSVESTSSATLSQLESSLAEDSHLGPSQSSFHQVPQGPSQSVDSYRH

Target ORF information:

RefSeq Version	XM_025282347.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centriolin (CNTRL), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025282347.1

ORF Insert Sequence:

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCTAAGACA GAAGACCATG 
GAACTAACGC GAGCATGTCA AAAACAGTAT GAACTGGAAC AGGAGTTGGC TTTTTACAAA 
ATCGATGCTA AATTTGAGCC ACTGAATTAT TATCCCTCAG AGTATGTTGA CGTTGATAAA 
GCCCCAGATG AAAGCCCTTA CATTGGCAAG TCCAGATACA AGAGAAATAT GTTCGCCACA 
GAGAGTTACA TTGTGAATAA TGCCCAGCCA GTTGAGATCA AGAGGATGGA GCCAGAGGAA 
GGGGAGCAAC TTAGAAACGA GCATGTGAGC TTGAAAGCCC ATATGCCACT GGACACGGAG 
CTGGAAGACA AAGAGAACAA AATAAGTACA GCACAAGCTC GACTATCAGC ACTGCATGAT 
GAAATAGAAA AGGCAGAACA ACAAATTTTA AGAGCCACTG AAGAATTTAA ACAACTGGAA 
GAAGCTGTAC AACTTAAAAA GATTTCAGAA GCAGAGAAAG ACTTTCTTCT CAAGCAGCTG 
AGTGGCAGGA TACAGCTTCT AAATAAATTA CGCCAGGAAG CTCTTGATCT AGAAATGCAG 
ATGGAAAAGC AAAGAGGAGA AATTGCTGAA AAGCAGGAGG AAATCAAGGA CCTCCAAAGA 
GTTATTGATA GTCTGGATTC CAAAGACCCA AAACATTGCC ATATGAAGGC TCAGAAAAGG 
GGTAAAGAAC AACAATTTGA CATTATGAAC AAGCAGTACA AACAACTTGA GAGCCGTTTG 
GACGAGATAC TTTCTAGAAT TGCTAAAGAA ACTGAAGAGA TTAAGGACCT TGAACAACAG 
CTTACTGAAG GCCAGATAGC AGCAAATGAA GCCCTGAAGA AGGACTTAGA AGGCGTCATC 
AGTGGCCTGC AAGAATACCT GGGCACTGTG AAGGGCCAGG CCGCTCAGGC CCAGAGCGAG 
TGCAGAAGGC TGCAGGATGA GAAAGAGGCG CTGCTGCAGA GGTTGGCCGA GGTCGAGCAG 
GAAAGGGACC AGCTGGAAGC AGTCGCCTTA GATGCAGAAG CTATGAGGAA GGAGCTTGCA 
GAGCTAGAAA GCGCCCTCCA AGAGCAGCAT GAGGTGAACG CGTCCCTGCA GCAGACCCAC 
GGAGACCTCA GTGCCTACGA GGCTGAGCTA GAGGCACAGC TGAGGATGAG GGATGCCGAA 
GCCAGCCAGC TCAAAGAAGA GCTGGGAGAA CTAACAAGGC TCAGCCAGCT AGAACAGTCA 
ACTCTACAAA CAGAACTTGA GAAAGAAAAA CAAGCCCTCA AGAACGCCCT GGGAAAAGCC 
CAGCTCTCGG AAGAAAAAGA ACAAGAAAAC AGTGAGCTCC GCACCCAGCT TAAACAGCTA 
CAGGATGACA ATAACCTGTT AAAACAGCAA CTTAAAGATT TCCAGAATCA CCTTAATCAG 
GTAGCTGATG GTCTGATTCG TCCAGAAGAA GTGGCAGCTC GTGTAGATGA AGTAAGGAGA 
AAATTGAAAT CAGGCGCTGG AGAGATGAGC AGAATCCACA GTCCTTCAGA TGTCTTAGGG 
AAAAGTCTTG CTGACTTGCA GATACAATTC AGTGAAATGC TGACACGTTC TCAGTGGGAG 
AAAGAGGAAG CCCAAGGGAG GGAAAGAAAA CTCCAAGAAG AAATGGCTCT GCATCAGGAG 
AAGCTGGTGG ATGGACAGGA AGCGTTCAGG CAGGCCTGTG AGAGAGCGCT AGAAGCAAGG 
ATTAATTTTG ACAAGAGGCA ACATGAAGCA AGAATTCAGC AGTTAGAGAA TGAGATTCAC 
TATTTACAAG AAAATCTAAA AAGTATGGAG GAAATCCAAG GGCTTACAGA TCTGCAGCTG 
CAGGAAGCTG ATGAAGAGAA GGAGAGAATT CTAGCCCAAC TCCAGGAGTT AGAGAAGAAG 
AAGAAACTTG AAGATGCCAA ATCTCAGGCA CAGGTTCTAG GTTTAGATAA AGAATTGAAG 
AAACTAAAGA AGGCTGTGGC CACCTCTGAT AAGATAGCCA CAGCTGAGCT CACCATCGCC 
AAAGACCAGC TCAAGTCCCT TCATGGAACT GTTATGAAAA TTAACCAGGA GCGTGCAGAG 
GACCTGCAGG AAGCAGAGCG GTTCAGCAGA AAGGCAGCAC AGGCCGCGAG GGATCTGACC 
CGAGCGGAAG CGGAGATCGA ACTCCTGCAG AACCTCCTCA GGGAGAGAGA GGAGCAGATG 
GAGAAGGTAG AGGCAGGCAC CCGAGGAGCA AGCTCACAGG CGCTGGAAAT CGAGAGGTGG 
AATGAGACCA TGGAGAGACA GAGGAGCGAG ATCGCAAGGC TACGGGACTC ACTAGACCGC 
ACCGGGGCGG ATAACAGAGG AGGCATTGAA AATATTTTGG AAGAAATAGC TGAACTCCGA 
CGTGAAGTTT CTTACCAGAA TGATTACATC AGCAGCATGG CAGATCCTTT CAAAAGACGA 
GGCTATTGGT ACTTTATGCC ACCACCATCA TCATCAAAAG TTTCCAGCCA CAGCTCCCAG 
GCCACAAAGG ACTCTGGTGT CGGGATGAAG TACGCAGCCT CGACTCCTAT TAGAAAACCA 
CAGCAGAACG GGAGGGAAGA CGGTGCCCCT CCAGCTGCCT CCGGATACTG GGTGTATTCC 
CCCATCAGGA GCGGCTTACA TAAATCGTTT TCAAATAAAG ATGCAGACAG TGGAGGAGAG 
AGCCGGGAGG AGAGCGAGCT GGATGACCAC GATGACCGCC CGTTCGTCCC TCCTCCTGGA 
TACATGATGT ACACCGTGTT CCCCGACGGC TCTCCTGTAC CACAGGGCAT GGCCCTCTAC 
GCACCGCCGC CTCCCTTGCC CAACAATAGC CGACCTCTCA GCCCTGGCAC TGTTGTGTAT 
GGCCCGCCCC CTGCTGGTGC CCCCATTGTC TACGGGCCCC CACCGCCCAG CTTCTCCGTC 
CCCCTCATCC CAGTGGGCGT GCTGCACTGC AACGTCCCGG AGCACCATGC CTTAGAGAAT 
GAGGTTTCTA GATTGGAAGA CATAATACAG CATTTAAAAT CAAAGAAGCA GGAACAGAGG 
ATGAGAGTAT GCAAGCGGCA GTCTGAGAAA GAAATAGAAG AACTGCATCA TAATATTGAT 
GAACTTTTGC AAGAGAAGAA AGACTTAGAG CACGAAGTAG AAGAATTACA TAGAACCATC 
CAGAAACATC AACAGCAAAA AGACTTCATT GATGGAAATG TTGAGAGTCT TATGAATGAA 
CTAAAAATAG AAAAATCACT CAAGCACCAT GAAGATATTG TAGATGAAAT TGAGTGCATC 
GAGAAGACTC TCCTGAAACG TCGAGCAGAG CTCAGGGAGG CTGACCGACT GCTGGCGGAA 
GCCGAGAGTG AACTTTCACG CACAACAGAG AAAACAAAAA ATGCTGTTGA AAAGTTCACT 
GATGCCAAGA GGAATTTGTT GCAAACTGAG AAAGATGCTG AGGAGTTAGA AAGGAGAGCT 
CAGGAAACAG CTGTTAGCCT TGTCAAGGCT GATCAACAGC TCAGATTGTT ACAGGCGGAC 
ACAAAGGATT TGGAGCAGCA CAAAACTGAG CAAGAAGAGA TCTTGAAAGA GATAAACAAA 
GTTGTTGCAG CAAAAGACTC AGACTTCCAG TGTTTAAACA CGAAGAAGGA GAAGCTAACA 
GAAGAGCTGC AGAAGCTGCA GAAAGACATC GAGACGGCAG CGCGCACGGA GGATCACCAC 
CTGCAGGTCC TGAGAGAGTC GGAGGCCCTC CTGCAGGCCA AGAGAGCGGA GCTGGACAAG 
CTGAGGAGCC AGGTAACGAG TCAGCAGCAG GAGATGGCTG TCTTGGACAG ACAGCTGGGG 
CATAAGAAGG AGGAGCTGCA TCTACTCCAG GGAAGCATGG TCCAGGCCAG AGCTGACCTC 
CAGGAAGCGC TGAGGCTGGG AGAAACTGAA GTAACTGAGA AGTGCAACCA CATCAGGGAA 
CTAAAAGGTC TTCTGGAAGA ACTGAGTTTT CAGAAAGGAG AACTGAATGT CCAGATTAGT 
GAGAAAAAAA ATCAACTTTC ACTTATAAAG CAAGAAATTG AAAAAGAGGA AGAAAATCTT 
CAGGTAGTTT TAGGGCAGAT GTCTAAACAT AAAACTGAAC TTAAGAATAT TCTGGACATG 
TTGCAACTTG AAAACAATGA GCTGCAAGCT TTGAAGCTAC AGCATGAGCA AAAGATGTCT 
GAGTTAGAGA AGGCTCAGGT TGAAGTGCTG GAGGAGAAAC TGGAGTTGGA GAATTTGCAG 
CAGACAGCCC TGCGACAGAA AGGGGAGATC GAGTGGCAGA AGCAGCTCTT AGAGAGGGAC 
AAGCGAGAGA TTGAGCGGAT AACTGCTGAG ACCCGAGCAT TGCAGTTGTG TGTTGAGTCT 
TTGTGCAAAC AAAAGGAAGA TCTTGAGGAG AAATGTGACA GCTGGGAGAA GAAGCTGGCA 
CAGACCAAAC GGGTTTTAGC AGCCACAGAA GAGAATAGCA AAGCAAAGCA ATCAGACTTA 
GAAAAGTTGG AATTGGATAT CAGAAAACTG GAACAGGAAC TAGACCAGCT AAACAGAAAC 
AAACTCTCAC TGCACAGAGA CATCGCGGCC ATGCAGCAGC AGGTCCAAGA AAAACGAGAA 
GCAGTAAACT CATTACAGGA GGAACTAGCT GATGTCCAAG CCCATTTGAA CCTAGCGAAA 
CAGGACCTGC TCCACACCAC CAAGCGCCAG GATGTCCTGC TTGGCGAGCA GACCAGGCTG 
CAGGAGGACC TCCAGGAACG CAGAAAGAAG TTGGAAGTCT GCCAAAAAGA AGAGGCAACA 
AAGCGGCAGC ACCTGCAAGC GCTTCAGACG GAGATGGAGG CGAACGAGGC CGAGCTGGCC 
CGGCAGGAGA CGGTACCGCA CCCGCGGGCA CGCGCGGGGG GGCACTGCCA CGCGCAGAGC 
GCTCTTCCCC TGGGGTCTGA GGTGGAGGCG AACGAGAGCG AGCTGGCCCG GCAGGAGACG 
ACATTTCAGA GACTCCAGAA AGAGAGAGAA AGTGAAGAAA AAAAGTTAGA AGCCAGTCGA 
GCGACTCTGC AGGAGCAGCA GCAGCAGCTG GAAAAGGAAA TCGCAGACCA GAAGAACGTG 
CTGGACCAGG TGCTCAGCCA GGTGCTGGGG GCCAAGGAGC GCGCCAGGAC TCTGCAGCAG 
GAGGAGAGGT GGGGTGAGAC CCTGGAGAAG ACGCTCTCCC AGACCCAGCA ACAGCTCTCA 
GAACGGGAGC AGCAGTTGTT GGAAAAGTCA GCTGAGCTGC TGGCTCTTCA GAAAGAGGCA 
GACTCGATGA GGGCAGACTT CAGCCTCTTG CGAAACCAGT TCTTGACGGA GAGGAAGAAA 
GCCGAGAAGC AGGTAGCCAG CCTCAGGGAA GCGCTCAAGA CCCAGCGCAG CCAGCTGGAG 
AAGACCCTTC TCGAGCAGAA GCAGGAGAAC AGCTCCATGC AGAAGGAGAT GGCGACTATC 
GAGCAGGTGG CGCAGGACAA CCACGAGCGG GCCCGGCGCC TGATGCGGGA GCTCAGCCAG 
ATGCAGCAGG AGTACGCGGA GCTCAAGAAG CAGATGGTAA ACCAGAAAGA TCTGGAAAGA 
AGACAAGTAG AAATCAGTGA AGCGATGAGG ACACTTAAGT CTGAAGTGAA AGACGAGATC 
AGAGCCAGCC TGAAGAACCT CAACCAGTTT CTCCCGGAGC TGCCAGCAGA CCTGGAGGCC 
ATTCTGGAAA GGAGCGAACA CCTAGACGGA GGGCTGGAGA GCTTGAAGGA GAACTTTCCA 
TTCGCCCTGA GTGAGAAGCC ATCACCTTTG GAAGAGAAAC TACATTTTTC TCGCGTTCAC 
ATAATGGATG AGCACTGGCG TGGAGAAGCG CTCCGGGAGA AGCTGCGGCA CCGTGAGGAC 
CGACTCAAGG CCCAGCTCCG ACACTGTATG TCCAAGCAGG CAGAGGTGTT AAGCAAAGGG 
AAGCGGCAGA CCGAGGGCAC GTTACACAGC CTGAGGAGGC AGGTCGACGC CTTAGGGGAG 
CTGGTCACCA GCACGTCTGT GGAGTCCACG TCGTCAGCCA CTCTGTCTCA GCTCGAATCT 
TCCCTTGCAG AGGACTCCCA TCTTGGACCA AGTCAGAGCT CCTTCCATCA AGTTCCACAA 
GGTCCATCAC AATCAGTAGA CAGTTACCGA CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTRL ( XM_025282348.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025138133.1 Bubalus bubalis centriolin (CNTRL), transcript variant X6, mRNA.

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CloneID	OBu63436
Clone ID Related Accession (Same CDS sequence)	XM_025282348.1
Accession Version	XM_025282348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	centriolin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCTAAGACA GAAGACCATG 
GAACTAACGC GAGCATGTCA AAAACAGTAT GAACTGGAAC AGGAGTTGGC TTTTTACAAA 
ATCGATGCTA AATTTGAGCC ACTGAATTAT TATCCCTCAG AGTATGTTGA CGTTGATAAA 
GCCCCAGATG AAAGCCCTTA CATTGGCAAG TCCAGATACA AGAGAAATAT GTTCGCCACA 
GAGAGTTACA TTGTGAATAA TGCCCAGCCA GTTGAGATCA AGAGGATGGA GCCAGAGGAA 
GGGGAGCAAC TTAGAAACGA GCATGTGAGC TTGAAAGCCC ATATGCCACT GGACACGGAG 
CTGGAAGACA AAGAGAACAA AATAAGTACA GCACAAGCTC GACTATCAGC ACTGCATGAT 
GAAATAGAAA AGGCAGAACA ACAAATTTTA AGAGCCACTG AAGAATTTAA ACAACTGGAA 
GAAGCTGTAC AACTTAAAAA GATTTCAGAA GCAGAGAAAG ACTTTCTTCT CAAGCAGCTG 
AGTGGCAGGA TACAGCTTCT AAATAAATTA CGCCAGGAAG CTCTTGATCT AGAAATGCAG 
ATGGAAAAGC AAAGAGGAGA AATTGCTGAA AAGCAGGAGG AAATCAAGGA CCTCCAAAGA 
GTTATTGATA GTCTGGATTC CAAAGACCCA AAACATTGCC ATATGAAGGC TCAGAAAAGG 
GGTAAAGAAC AACAATTTGA CATTATGAAC AAGCAGTACA AACAACTTGA GAGCCGTTTG 
GACGAGATAC TTTCTAGAAT TGCTAAAGAA ACTGAAGAGA TTAAGGACCT TGAACAACAG 
CTTACTGAAG GCCAGATAGC AGCAAATGAA GCCCTGAAGA AGGACTTAGA AGGCGTCATC 
AGTGGCCTGC AAGAATACCT GGGCACTGTG AAGGGCCAGG CCGCTCAGGC CCAGAGCGAG 
TGCAGAAGGC TGCAGGATGA GAAAGAGGCG CTGCTGCAGA GGTTGGCCGA GGTCGAGCAG 
GAAAGGGACC AGCTGGAAGC AGTCGCCTTA GATGCAGAAG CTATGAGGAA GGAGCTTGCA 
GAGCTAGAAA GCGCCCTCCA AGAGCAGCAT GAGGTGAACG CGTCCCTGCA GCAGACCCAC 
GGAGACCTCA GTGCCTACGA GGCTGAGCTA GAGGCACAGC TGAGGATGAG GGATGCCGAA 
GCCAGCCAGC TCAAAGAAGA GCTGGGAGAA CTAACAAGGC TCAGCCAGCT AGAACAGTCA 
ACTCTACAAA CAGAACTTGA GAAAGAAAAA CAAGCCCTCA AGAACGCCCT GGGAAAAGCC 
CAGCTCTCGG AAGAAAAAGA ACAAGAAAAC AGTGAGCTCC GCACCCAGCT TAAACAGCTA 
CAGGATGACA ATAACCTGTT AAAACAGCAA CTTAAAGATT TCCAGAATCA CCTTAATCAG 
GTAGCTGATG GTCTGATTCG TCCAGAAGAA GTGGCAGCTC GTGTAGATGA AGTAAGGAGA 
AAATTGAAAT CAGGCGCTGG AGAGATGAGA ATCCACAGTC CTTCAGATGT CTTAGGGAAA 
AGTCTTGCTG ACTTGCAGAT ACAATTCAGT GAAATGCTGA CACGTTCTCA GTGGGAGAAA 
GAGGAAGCCC AAGGGAGGGA AAGAAAACTC CAAGAAGAAA TGGCTCTGCA TCAGGAGAAG 
CTGGTGGATG GACAGGAAGC GTTCAGGCAG GCCTGTGAGA GAGCGCTAGA AGCAAGGATT 
AATTTTGACA AGAGGCAACA TGAAGCAAGA ATTCAGCAGT TAGAGAATGA GATTCACTAT 
TTACAAGAAA ATCTAAAAAG TATGGAGGAA ATCCAAGGGC TTACAGATCT GCAGCTGCAG 
GAAGCTGATG AAGAGAAGGA GAGAATTCTA GCCCAACTCC AGGAGTTAGA GAAGAAGAAG 
AAACTTGAAG ATGCCAAATC TCAGGCACAG GTTCTAGGTT TAGATAAAGA ATTGAAGAAA 
CTAAAGAAGG CTGTGGCCAC CTCTGATAAG ATAGCCACAG CTGAGCTCAC CATCGCCAAA 
GACCAGCTCA AGTCCCTTCA TGGAACTGTT ATGAAAATTA ACCAGGAGCG TGCAGAGGAC 
CTGCAGGAAG CAGAGCGGTT CAGCAGAAAG GCAGCACAGG CCGCGAGGGA TCTGACCCGA 
GCGGAAGCGG AGATCGAACT CCTGCAGAAC CTCCTCAGGG AGAGAGAGGA GCAGATGGAG 
AAGGTAGAGG CAGGCACCCG AGGAGCAAGC TCACAGGCGC TGGAAATCGA GAGGTGGAAT 
GAGACCATGG AGAGACAGAG GAGCGAGATC GCAAGGCTAC GGGACTCACT AGACCGCACC 
GGGGCGGATA ACAGAGGAGG CATTGAAAAT ATTTTGGAAG AAATAGCTGA ACTCCGACGT 
GAAGTTTCTT ACCAGAATGA TTACATCAGC AGCATGGCAG ATCCTTTCAA AAGACGAGGC 
TATTGGTACT TTATGCCACC ACCATCATCA TCAAAAGTTT CCAGCCACAG CTCCCAGGCC 
ACAAAGGACT CTGGTGTCGG GATGAAGTAC GCAGCCTCGA CTCCTATTAG AAAACCACAG 
CAGAACGGGA GGGAAGACGG TGCCCCTCCA GCTGCCTCCG GATACTGGGT GTATTCCCCC 
ATCAGGAGCG GCTTACATAA ATCGTTTTCA AATAAAGATG CAGACAGTGG AGGAGAGAGC 
CGGGAGGAGA GCGAGCTGGA TGACCACGAT GACCGCCCGT TCGTCCCTCC TCCTGGATAC 
ATGATGTACA CCGTGTTCCC CGACGGCTCT CCTGTACCAC AGGGCATGGC CCTCTACGCA 
CCGCCGCCTC CCTTGCCCAA CAATAGCCGA CCTCTCAGCC CTGGCACTGT TGTGTATGGC 
CCGCCCCCTG CTGGTGCCCC CATTGTCTAC GGGCCCCCAC CGCCCAGCTT CTCCGTCCCC 
CTCATCCCAG TGGGCGTGCT GCACTGCAAC GTCCCGGAGC ACCATGCCTT AGAGAATGAG 
GTTTCTAGAT TGGAAGACAT AATACAGCAT TTAAAATCAA AGAAGCAGGA ACAGAGGATG 
AGAGTATGCA AGCGGCAGTC TGAGAAAGAA ATAGAAGAAC TGCATCATAA TATTGATGAA 
CTTTTGCAAG AGAAGAAAGA CTTAGAGCAC GAAGTAGAAG AATTACATAG AACCATCCAG 
AAACATCAAC AGCAAAAAGA CTTCATTGAT GGAAATGTTG AGAGTCTTAT GAATGAACTA 
AAAATAGAAA AATCACTCAA GCACCATGAA GATATTGTAG ATGAAATTGA GTGCATCGAG 
AAGACTCTCC TGAAACGTCG AGCAGAGCTC AGGGAGGCTG ACCGACTGCT GGCGGAAGCC 
GAGAGTGAAC TTTCACGCAC AACAGAGAAA ACAAAAAATG CTGTTGAAAA GTTCACTGAT 
GCCAAGAGGA ATTTGTTGCA AACTGAGAAA GATGCTGAGG AGTTAGAAAG GAGAGCTCAG 
GAAACAGCTG TTAGCCTTGT CAAGGCTGAT CAACAGCTCA GATTGTTACA GGCGGACACA 
AAGGATTTGG AGCAGCACAA AACTGAGCAA GAAGAGATCT TGAAAGAGAT AAACAAAGTT 
GTTGCAGCAA AAGACTCAGA CTTCCAGTGT TTAAACACGA AGAAGGAGAA GCTAACAGAA 
GAGCTGCAGA AGCTGCAGAA AGACATCGAG ACGGCAGCGC GCACGGAGGA TCACCACCTG 
CAGGTCCTGA GAGAGTCGGA GGCCCTCCTG CAGGCCAAGA GAGCGGAGCT GGACAAGCTG 
AGGAGCCAGG TAACGAGTCA GCAGCAGGAG ATGGCTGTCT TGGACAGACA GCTGGGGCAT 
AAGAAGGAGG AGCTGCATCT ACTCCAGGGA AGCATGGTCC AGGCCAGAGC TGACCTCCAG 
GAAGCGCTGA GGCTGGGAGA AACTGAAGTA ACTGAGAAGT GCAACCACAT CAGGGAACTA 
AAAGGTCTTC TGGAAGAACT GAGTTTTCAG AAAGGAGAAC TGAATGTCCA GATTAGTGAG 
AAAAAAAATC AACTTTCACT TATAAAGCAA GAAATTGAAA AAGAGGAAGA AAATCTTCAG 
GTAGTTTTAG GGCAGATGTC TAAACATAAA ACTGAACTTA AGAATATTCT GGACATGTTG 
CAACTTGAAA ACAATGAGCT GCAAGCTTTG AAGCTACAGC ATGAGCAAAA GATGTCTGAG 
TTAGAGAAGG CTCAGGTTGA AGTGCTGGAG GAGAAACTGG AGTTGGAGAA TTTGCAGCAG 
ACAGCCCTGC GACAGAAAGG GGAGATCGAG TGGCAGAAGC AGCTCTTAGA GAGGGACAAG 
CGAGAGATTG AGCGGATAAC TGCTGAGACC CGAGCATTGC AGTTGTGTGT TGAGTCTTTG 
TGCAAACAAA AGGAAGATCT TGAGGAGAAA TGTGACAGCT GGGAGAAGAA GCTGGCACAG 
ACCAAACGGG TTTTAGCAGC CACAGAAGAG AATAGCAAAG CAAAGCAATC AGACTTAGAA 
AAGTTGGAAT TGGATATCAG AAAACTGGAA CAGGAACTAG ACCAGCTAAA CAGAAACAAA 
CTCTCACTGC ACAGAGACAT CGCGGCCATG CAGCAGCAGG TCCAAGAAAA ACGAGAAGCA 
GTAAACTCAT TACAGGAGGA ACTAGCTGAT GTCCAAGCCC ATTTGAACCT AGCGAAACAG 
GACCTGCTCC ACACCACCAA GCGCCAGGAT GTCCTGCTTG GCGAGCAGAC CAGGCTGCAG 
GAGGACCTCC AGGAACGCAG AAAGAAGTTG GAAGTCTGCC AAAAAGAAGA GGCAACAAAG 
CGGCAGCACC TGCAAGCGCT TCAGACGGAG ATGGAGGCGA ACGAGGCCGA GCTGGCCCGG 
CAGGAGACGG TACCGCACCC GCGGGCACGC GCGGGGGGGC ACTGCCACGC GCAGAGCGCT 
CTTCCCCTGG GGTCTGAGGT GGAGGCGAAC GAGAGCGAGC TGGCCCGGCA GGAGACGACA 
TTTCAGAGAC TCCAGAAAGA GAGAGAAAGT GAAGAAAAAA AGTTAGAAGC CAGTCGAGCG 
ACTCTGCAGG AGCAGCAGCA GCAGCTGGAA AAGGAAATCG CAGACCAGAA GAACGTGCTG 
GACCAGGTGC TCAGCCAGGT GCTGGGGGCC AAGGAGCGCG CCAGGACTCT GCAGCAGGAG 
GAGAGGTGGG GTGAGACCCT GGAGAAGACG CTCTCCCAGA CCCAGCAACA GCTCTCAGAA 
CGGGAGCAGC AGTTGTTGGA AAAGTCAGCT GAGCTGCTGG CTCTTCAGAA AGAGGCAGAC 
TCGATGAGGG CAGACTTCAG CCTCTTGCGA AACCAGTTCT TGACGGAGAG GAAGAAAGCC 
GAGAAGCAGG TAGCCAGCCT CAGGGAAGCG CTCAAGACCC AGCGCAGCCA GCTGGAGAAG 
ACCCTTCTCG AGCAGAAGCA GGAGAACAGC TCCATGCAGA AGGAGATGGC GACTATCGAG 
CAGGTGGCGC AGGACAACCA CGAGCGGGCC CGGCGCCTGA TGCGGGAGCT CAGCCAGATG 
CAGCAGGAGT ACGCGGAGCT CAAGAAGCAG ATGGTAAACC AGAAAGATCT GGAAAGAAGA 
CAAGTAGAAA TCAGTGAAGC GATGAGGACA CTTAAGTCTG AAGTGAAAGA CGAGATCAGA 
GCCAGCCTGA AGAACCTCAA CCAGTTTCTC CCGGAGCTGC CAGCAGACCT GGAGGCCATT 
CTGGAAAGGA GCGAACACCT AGACGGAGGG CTGGAGAGCT TGAAGGAGAA CTTTCCATTC 
GCCCTGAGTG AGAAGCCATC ACCTTTGGAA GAGAAACTAC ATTTTTCTCG CGTTCACATA 
ATGGATGAGC ACTGGCGTGG AGAAGCGCTC CGGGAGAAGC TGCGGCACCG TGAGGACCGA 
CTCAAGGCCC AGCTCCGACA CTGTATGTCC AAGCAGGCAG AGGTGTTAAG CAAAGGGAAG 
CGGCAGACCG AGGGCACGTT ACACAGCCTG AGGAGGCAGG TCGACGCCTT AGGGGAGCTG 
GTCACCAGCA CGTCTGTGGA GTCCACGTCG TCAGCCACTC TGTCTCAGCT CGAATCTTCC 
CTTGCAGAGG ACTCCCATCT TGGACCAAGT CAGAGCTCCT TCCATCAAGT TCCACAAGGT 
CCATCACAAT CAGTAGACAG TTACCGACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025138133.1
CDS	366..7478
Translation	MKKGPQQKISKTKMPPSSHSPSPSSLTSNMRSRSLSPLNGSETLPFHSGRQWYGQVEIIGENSMLLDYHDQKEADSHAGVRYITEALVKKLTKQENLTLVKSLNLSLSKDGGKKFKYIENLEKCTKLEILNLSHNLIGKIEKVDKLLKLRELNLSYNKICKIEGIENMHNLQKLNLAGNEIEHIPAWLGKKLKCLRVLNLKANKISSLQDVSKLKPLQDLTSLILAENPVVTLPHYLQFTIFHLRSLESLEGQPVTTQDRQEAFERFSLEEVERLERDLEKKMMETEELKSKQAQFLEEIKNQDKLNRSLKEEAMLQKQSCEELESNLNTKNELLRQKTMELTRACQKQYELEQELAFYKIDAKFEPLNYYPSEYVDVDKAPDESPYIGKSRYKRNMFATESYIVNNAQPVEIKRMEPEEGEQLRNEHVSLKAHMPLDTELEDKENKISTAQARLSALHDEIEKAEQQILRATEEFKQLEEAVQLKKISEAEKDFLLKQLSGRIQLLNKLRQEALDLEMQMEKQRGEIAEKQEEIKDLQRVIDSLDSKDPKHCHMKAQKRGKEQQFDIMNKQYKQLESRLDEILSRIAKETEEIKDLEQQLTEGQIAANEALKKDLEGVISGLQEYLGTVKGQAAQAQSECRRLQDEKEALLQRLAEVEQERDQLEAVALDAEAMRKELAELESALQEQHEVNASLQQTHGDLSAYEAELEAQLRMRDAEASQLKEELGELTRLSQLEQSTLQTELEKEKQALKNALGKAQLSEEKEQENSELRTQLKQLQDDNNLLKQQLKDFQNHLNQVADGLIRPEEVAARVDEVRRKLKSGAGEMRIHSPSDVLGKSLADLQIQFSEMLTRSQWEKEEAQGRERKLQEEMALHQEKLVDGQEAFRQACERALEARINFDKRQHEARIQQLENEIHYLQENLKSMEEIQGLTDLQLQEADEEKERILAQLQELEKKKKLEDAKSQAQVLGLDKELKKLKKAVATSDKIATAELTIAKDQLKSLHGTVMKINQERAEDLQEAERFSRKAAQAARDLTRAEAEIELLQNLLREREEQMEKVEAGTRGASSQALEIERWNETMERQRSEIARLRDSLDRTGADNRGGIENILEEIAELRREVSYQNDYISSMADPFKRRGYWYFMPPPSSSKVSSHSSQATKDSGVGMKYAASTPIRKPQQNGREDGAPPAASGYWVYSPIRSGLHKSFSNKDADSGGESREESELDDHDDRPFVPPPGYMMYTVFPDGSPVPQGMALYAPPPPLPNNSRPLSPGTVVYGPPPAGAPIVYGPPPPSFSVPLIPVGVLHCNVPEHHALENEVSRLEDIIQHLKSKKQEQRMRVCKRQSEKEIEELHHNIDELLQEKKDLEHEVEELHRTIQKHQQQKDFIDGNVESLMNELKIEKSLKHHEDIVDEIECIEKTLLKRRAELREADRLLAEAESELSRTTEKTKNAVEKFTDAKRNLLQTEKDAEELERRAQETAVSLVKADQQLRLLQADTKDLEQHKTEQEEILKEINKVVAAKDSDFQCLNTKKEKLTEELQKLQKDIETAARTEDHHLQVLRESEALLQAKRAELDKLRSQVTSQQQEMAVLDRQLGHKKEELHLLQGSMVQARADLQEALRLGETEVTEKCNHIRELKGLLEELSFQKGELNVQISEKKNQLSLIKQEIEKEEENLQVVLGQMSKHKTELKNILDMLQLENNELQALKLQHEQKMSELEKAQVEVLEEKLELENLQQTALRQKGEIEWQKQLLERDKREIERITAETRALQLCVESLCKQKEDLEEKCDSWEKKLAQTKRVLAATEENSKAKQSDLEKLELDIRKLEQELDQLNRNKLSLHRDIAAMQQQVQEKREAVNSLQEELADVQAHLNLAKQDLLHTTKRQDVLLGEQTRLQEDLQERRKKLEVCQKEEATKRQHLQALQTEMEANEAELARQETVPHPRARAGGHCHAQSALPLGSEVEANESELARQETTFQRLQKERESEEKKLEASRATLQEQQQQLEKEIADQKNVLDQVLSQVLGAKERARTLQQEERWGETLEKTLSQTQQQLSEREQQLLEKSAELLALQKEADSMRADFSLLRNQFLTERKKAEKQVASLREALKTQRSQLEKTLLEQKQENSSMQKEMATIEQVAQDNHERARRLMRELSQMQQEYAELKKQMVNQKDLERRQVEISEAMRTLKSEVKDEIRASLKNLNQFLPELPADLEAILERSEHLDGGLESLKENFPFALSEKPSPLEEKLHFSRVHIMDEHWRGEALREKLRHREDRLKAQLRHCMSKQAEVLSKGKRQTEGTLHSLRRQVDALGELVTSTSVESTSSATLSQLESSLAEDSHLGPSQSSFHQVPQGPSQSVDSYRH

Target ORF information:

RefSeq Version	XM_025282348.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centriolin (CNTRL), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025282348.1

ORF Insert Sequence:

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCTAAGACA GAAGACCATG 
GAACTAACGC GAGCATGTCA AAAACAGTAT GAACTGGAAC AGGAGTTGGC TTTTTACAAA 
ATCGATGCTA AATTTGAGCC ACTGAATTAT TATCCCTCAG AGTATGTTGA CGTTGATAAA 
GCCCCAGATG AAAGCCCTTA CATTGGCAAG TCCAGATACA AGAGAAATAT GTTCGCCACA 
GAGAGTTACA TTGTGAATAA TGCCCAGCCA GTTGAGATCA AGAGGATGGA GCCAGAGGAA 
GGGGAGCAAC TTAGAAACGA GCATGTGAGC TTGAAAGCCC ATATGCCACT GGACACGGAG 
CTGGAAGACA AAGAGAACAA AATAAGTACA GCACAAGCTC GACTATCAGC ACTGCATGAT 
GAAATAGAAA AGGCAGAACA ACAAATTTTA AGAGCCACTG AAGAATTTAA ACAACTGGAA 
GAAGCTGTAC AACTTAAAAA GATTTCAGAA GCAGAGAAAG ACTTTCTTCT CAAGCAGCTG 
AGTGGCAGGA TACAGCTTCT AAATAAATTA CGCCAGGAAG CTCTTGATCT AGAAATGCAG 
ATGGAAAAGC AAAGAGGAGA AATTGCTGAA AAGCAGGAGG AAATCAAGGA CCTCCAAAGA 
GTTATTGATA GTCTGGATTC CAAAGACCCA AAACATTGCC ATATGAAGGC TCAGAAAAGG 
GGTAAAGAAC AACAATTTGA CATTATGAAC AAGCAGTACA AACAACTTGA GAGCCGTTTG 
GACGAGATAC TTTCTAGAAT TGCTAAAGAA ACTGAAGAGA TTAAGGACCT TGAACAACAG 
CTTACTGAAG GCCAGATAGC AGCAAATGAA GCCCTGAAGA AGGACTTAGA AGGCGTCATC 
AGTGGCCTGC AAGAATACCT GGGCACTGTG AAGGGCCAGG CCGCTCAGGC CCAGAGCGAG 
TGCAGAAGGC TGCAGGATGA GAAAGAGGCG CTGCTGCAGA GGTTGGCCGA GGTCGAGCAG 
GAAAGGGACC AGCTGGAAGC AGTCGCCTTA GATGCAGAAG CTATGAGGAA GGAGCTTGCA 
GAGCTAGAAA GCGCCCTCCA AGAGCAGCAT GAGGTGAACG CGTCCCTGCA GCAGACCCAC 
GGAGACCTCA GTGCCTACGA GGCTGAGCTA GAGGCACAGC TGAGGATGAG GGATGCCGAA 
GCCAGCCAGC TCAAAGAAGA GCTGGGAGAA CTAACAAGGC TCAGCCAGCT AGAACAGTCA 
ACTCTACAAA CAGAACTTGA GAAAGAAAAA CAAGCCCTCA AGAACGCCCT GGGAAAAGCC 
CAGCTCTCGG AAGAAAAAGA ACAAGAAAAC AGTGAGCTCC GCACCCAGCT TAAACAGCTA 
CAGGATGACA ATAACCTGTT AAAACAGCAA CTTAAAGATT TCCAGAATCA CCTTAATCAG 
GTAGCTGATG GTCTGATTCG TCCAGAAGAA GTGGCAGCTC GTGTAGATGA AGTAAGGAGA 
AAATTGAAAT CAGGCGCTGG AGAGATGAGA ATCCACAGTC CTTCAGATGT CTTAGGGAAA 
AGTCTTGCTG ACTTGCAGAT ACAATTCAGT GAAATGCTGA CACGTTCTCA GTGGGAGAAA 
GAGGAAGCCC AAGGGAGGGA AAGAAAACTC CAAGAAGAAA TGGCTCTGCA TCAGGAGAAG 
CTGGTGGATG GACAGGAAGC GTTCAGGCAG GCCTGTGAGA GAGCGCTAGA AGCAAGGATT 
AATTTTGACA AGAGGCAACA TGAAGCAAGA ATTCAGCAGT TAGAGAATGA GATTCACTAT 
TTACAAGAAA ATCTAAAAAG TATGGAGGAA ATCCAAGGGC TTACAGATCT GCAGCTGCAG 
GAAGCTGATG AAGAGAAGGA GAGAATTCTA GCCCAACTCC AGGAGTTAGA GAAGAAGAAG 
AAACTTGAAG ATGCCAAATC TCAGGCACAG GTTCTAGGTT TAGATAAAGA ATTGAAGAAA 
CTAAAGAAGG CTGTGGCCAC CTCTGATAAG ATAGCCACAG CTGAGCTCAC CATCGCCAAA 
GACCAGCTCA AGTCCCTTCA TGGAACTGTT ATGAAAATTA ACCAGGAGCG TGCAGAGGAC 
CTGCAGGAAG CAGAGCGGTT CAGCAGAAAG GCAGCACAGG CCGCGAGGGA TCTGACCCGA 
GCGGAAGCGG AGATCGAACT CCTGCAGAAC CTCCTCAGGG AGAGAGAGGA GCAGATGGAG 
AAGGTAGAGG CAGGCACCCG AGGAGCAAGC TCACAGGCGC TGGAAATCGA GAGGTGGAAT 
GAGACCATGG AGAGACAGAG GAGCGAGATC GCAAGGCTAC GGGACTCACT AGACCGCACC 
GGGGCGGATA ACAGAGGAGG CATTGAAAAT ATTTTGGAAG AAATAGCTGA ACTCCGACGT 
GAAGTTTCTT ACCAGAATGA TTACATCAGC AGCATGGCAG ATCCTTTCAA AAGACGAGGC 
TATTGGTACT TTATGCCACC ACCATCATCA TCAAAAGTTT CCAGCCACAG CTCCCAGGCC 
ACAAAGGACT CTGGTGTCGG GATGAAGTAC GCAGCCTCGA CTCCTATTAG AAAACCACAG 
CAGAACGGGA GGGAAGACGG TGCCCCTCCA GCTGCCTCCG GATACTGGGT GTATTCCCCC 
ATCAGGAGCG GCTTACATAA ATCGTTTTCA AATAAAGATG CAGACAGTGG AGGAGAGAGC 
CGGGAGGAGA GCGAGCTGGA TGACCACGAT GACCGCCCGT TCGTCCCTCC TCCTGGATAC 
ATGATGTACA CCGTGTTCCC CGACGGCTCT CCTGTACCAC AGGGCATGGC CCTCTACGCA 
CCGCCGCCTC CCTTGCCCAA CAATAGCCGA CCTCTCAGCC CTGGCACTGT TGTGTATGGC 
CCGCCCCCTG CTGGTGCCCC CATTGTCTAC GGGCCCCCAC CGCCCAGCTT CTCCGTCCCC 
CTCATCCCAG TGGGCGTGCT GCACTGCAAC GTCCCGGAGC ACCATGCCTT AGAGAATGAG 
GTTTCTAGAT TGGAAGACAT AATACAGCAT TTAAAATCAA AGAAGCAGGA ACAGAGGATG 
AGAGTATGCA AGCGGCAGTC TGAGAAAGAA ATAGAAGAAC TGCATCATAA TATTGATGAA 
CTTTTGCAAG AGAAGAAAGA CTTAGAGCAC GAAGTAGAAG AATTACATAG AACCATCCAG 
AAACATCAAC AGCAAAAAGA CTTCATTGAT GGAAATGTTG AGAGTCTTAT GAATGAACTA 
AAAATAGAAA AATCACTCAA GCACCATGAA GATATTGTAG ATGAAATTGA GTGCATCGAG 
AAGACTCTCC TGAAACGTCG AGCAGAGCTC AGGGAGGCTG ACCGACTGCT GGCGGAAGCC 
GAGAGTGAAC TTTCACGCAC AACAGAGAAA ACAAAAAATG CTGTTGAAAA GTTCACTGAT 
GCCAAGAGGA ATTTGTTGCA AACTGAGAAA GATGCTGAGG AGTTAGAAAG GAGAGCTCAG 
GAAACAGCTG TTAGCCTTGT CAAGGCTGAT CAACAGCTCA GATTGTTACA GGCGGACACA 
AAGGATTTGG AGCAGCACAA AACTGAGCAA GAAGAGATCT TGAAAGAGAT AAACAAAGTT 
GTTGCAGCAA AAGACTCAGA CTTCCAGTGT TTAAACACGA AGAAGGAGAA GCTAACAGAA 
GAGCTGCAGA AGCTGCAGAA AGACATCGAG ACGGCAGCGC GCACGGAGGA TCACCACCTG 
CAGGTCCTGA GAGAGTCGGA GGCCCTCCTG CAGGCCAAGA GAGCGGAGCT GGACAAGCTG 
AGGAGCCAGG TAACGAGTCA GCAGCAGGAG ATGGCTGTCT TGGACAGACA GCTGGGGCAT 
AAGAAGGAGG AGCTGCATCT ACTCCAGGGA AGCATGGTCC AGGCCAGAGC TGACCTCCAG 
GAAGCGCTGA GGCTGGGAGA AACTGAAGTA ACTGAGAAGT GCAACCACAT CAGGGAACTA 
AAAGGTCTTC TGGAAGAACT GAGTTTTCAG AAAGGAGAAC TGAATGTCCA GATTAGTGAG 
AAAAAAAATC AACTTTCACT TATAAAGCAA GAAATTGAAA AAGAGGAAGA AAATCTTCAG 
GTAGTTTTAG GGCAGATGTC TAAACATAAA ACTGAACTTA AGAATATTCT GGACATGTTG 
CAACTTGAAA ACAATGAGCT GCAAGCTTTG AAGCTACAGC ATGAGCAAAA GATGTCTGAG 
TTAGAGAAGG CTCAGGTTGA AGTGCTGGAG GAGAAACTGG AGTTGGAGAA TTTGCAGCAG 
ACAGCCCTGC GACAGAAAGG GGAGATCGAG TGGCAGAAGC AGCTCTTAGA GAGGGACAAG 
CGAGAGATTG AGCGGATAAC TGCTGAGACC CGAGCATTGC AGTTGTGTGT TGAGTCTTTG 
TGCAAACAAA AGGAAGATCT TGAGGAGAAA TGTGACAGCT GGGAGAAGAA GCTGGCACAG 
ACCAAACGGG TTTTAGCAGC CACAGAAGAG AATAGCAAAG CAAAGCAATC AGACTTAGAA 
AAGTTGGAAT TGGATATCAG AAAACTGGAA CAGGAACTAG ACCAGCTAAA CAGAAACAAA 
CTCTCACTGC ACAGAGACAT CGCGGCCATG CAGCAGCAGG TCCAAGAAAA ACGAGAAGCA 
GTAAACTCAT TACAGGAGGA ACTAGCTGAT GTCCAAGCCC ATTTGAACCT AGCGAAACAG 
GACCTGCTCC ACACCACCAA GCGCCAGGAT GTCCTGCTTG GCGAGCAGAC CAGGCTGCAG 
GAGGACCTCC AGGAACGCAG AAAGAAGTTG GAAGTCTGCC AAAAAGAAGA GGCAACAAAG 
CGGCAGCACC TGCAAGCGCT TCAGACGGAG ATGGAGGCGA ACGAGGCCGA GCTGGCCCGG 
CAGGAGACGG TACCGCACCC GCGGGCACGC GCGGGGGGGC ACTGCCACGC GCAGAGCGCT 
CTTCCCCTGG GGTCTGAGGT GGAGGCGAAC GAGAGCGAGC TGGCCCGGCA GGAGACGACA 
TTTCAGAGAC TCCAGAAAGA GAGAGAAAGT GAAGAAAAAA AGTTAGAAGC CAGTCGAGCG 
ACTCTGCAGG AGCAGCAGCA GCAGCTGGAA AAGGAAATCG CAGACCAGAA GAACGTGCTG 
GACCAGGTGC TCAGCCAGGT GCTGGGGGCC AAGGAGCGCG CCAGGACTCT GCAGCAGGAG 
GAGAGGTGGG GTGAGACCCT GGAGAAGACG CTCTCCCAGA CCCAGCAACA GCTCTCAGAA 
CGGGAGCAGC AGTTGTTGGA AAAGTCAGCT GAGCTGCTGG CTCTTCAGAA AGAGGCAGAC 
TCGATGAGGG CAGACTTCAG CCTCTTGCGA AACCAGTTCT TGACGGAGAG GAAGAAAGCC 
GAGAAGCAGG TAGCCAGCCT CAGGGAAGCG CTCAAGACCC AGCGCAGCCA GCTGGAGAAG 
ACCCTTCTCG AGCAGAAGCA GGAGAACAGC TCCATGCAGA AGGAGATGGC GACTATCGAG 
CAGGTGGCGC AGGACAACCA CGAGCGGGCC CGGCGCCTGA TGCGGGAGCT CAGCCAGATG 
CAGCAGGAGT ACGCGGAGCT CAAGAAGCAG ATGGTAAACC AGAAAGATCT GGAAAGAAGA 
CAAGTAGAAA TCAGTGAAGC GATGAGGACA CTTAAGTCTG AAGTGAAAGA CGAGATCAGA 
GCCAGCCTGA AGAACCTCAA CCAGTTTCTC CCGGAGCTGC CAGCAGACCT GGAGGCCATT 
CTGGAAAGGA GCGAACACCT AGACGGAGGG CTGGAGAGCT TGAAGGAGAA CTTTCCATTC 
GCCCTGAGTG AGAAGCCATC ACCTTTGGAA GAGAAACTAC ATTTTTCTCG CGTTCACATA 
ATGGATGAGC ACTGGCGTGG AGAAGCGCTC CGGGAGAAGC TGCGGCACCG TGAGGACCGA 
CTCAAGGCCC AGCTCCGACA CTGTATGTCC AAGCAGGCAG AGGTGTTAAG CAAAGGGAAG 
CGGCAGACCG AGGGCACGTT ACACAGCCTG AGGAGGCAGG TCGACGCCTT AGGGGAGCTG 
GTCACCAGCA CGTCTGTGGA GTCCACGTCG TCAGCCACTC TGTCTCAGCT CGAATCTTCC 
CTTGCAGAGG ACTCCCATCT TGGACCAAGT CAGAGCTCCT TCCATCAAGT TCCACAAGGT 
CCATCACAAT CAGTAGACAG TTACCGACAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTRL ( XM_025282349.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025138134.1 Bubalus bubalis centriolin (CNTRL), transcript variant X7, mRNA.

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CloneID	OBu63437
Clone ID Related Accession (Same CDS sequence)	XM_025282349.1
Accession Version	XM_025282349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	centriolin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCCCTTGTC TCGGTTTCTT 
GCGGACCCTT GTCTGCCGAT TCTTTATTCT TCCTTAAAGC TAAGACAGAA GACCATGGAA 
CTAACGCGAG CATGTCAAAA ACAGTATGAA CTGGAACAGG AGTTGGCTTT TTACAAAATC 
GATGCTAAAT TTGAGCCACT GAATTATTAT CCCTCAGAGT ATGTTGACGT TGATAAAGCC 
CCAGATGAAA GCCCTTACAT TGGCAAGTCC AGATACAAGA GAAATATGTT CGCCACAGAG 
AGTTACATTG TGAATAATGC CCAGCCAGTT GAGATCAAGA GGATGGAGCC AGAGGAAGGG 
GAGCAACTTA GAAACGAGCA TGTGAGCTTG AAAGCCCATA TGCCACTGGA CACGGAGCTG 
GAAGACAAAG AGAACAAAAT AAGTACAGCA CAAGCTCGAC TATCAGCACT GCATGATGAA 
ATAGAAAAGG CAGAACAACA AATTTTAAGA GCCACTGAAG AATTTAAACA ACTGGAAGAA 
GCTGTACAAC TTAAAAAGAT TTCAGAAGCA GAGAAAGACT TTCTTCTCAA GCAGCTGAGT 
GGCAGGATAC AGCTTCTAAA TAAATTACGC CAGGAAGCTC TTGATCTAGA AATGCAGATG 
GAAAAGCAAA GAGGAGAAAT TGCTGAAAAG CAGGAGGAAA TCAAGGACCT CCAAAGAGTT 
ATTGATAGTC TGGATTCCAA AGACCCAAAA CATTGCCATA TGAAGGCTCA GAAAAGGGGT 
AAAGAACAAC AATTTGACAT TATGAACAAG CAGTACAAAC AACTTGAGAG CCGTTTGGAC 
GAGATACTTT CTAGAATTGC TAAAGAAACT GAAGAGATTA AGGACCTTGA ACAACAGCTT 
ACTGAAGGCC AGATAGCAGC AAATGAAGCC CTGAAGAAGG ACTTAGAAGG CGTCATCAGT 
GGCCTGCAAG AATACCTGGG CACTGTGAAG GGCCAGGCCG CTCAGGCCCA GAGCGAGTGC 
AGAAGGCTGC AGGATGAGAA AGAGGCGCTG CTGCAGAGGT TGGCCGAGGT CGAGCAGGAA 
AGGGACCAGC TGGAAGCAGT CGCCTTAGAT GCAGAAGCTA TGAGGAAGGA GCTTGCAGAG 
CTAGAAAGCG CCCTCCAAGA GCAGCATGAG GTGAACGCGT CCCTGCAGCA GACCCACGGA 
GACCTCAGTG CCTACGAGGC TGAGCTAGAG GCACAGCTGA GGATGAGGGA TGCCGAAGCC 
AGCCAGCTCA AAGAAGAGCT GGGAGAACTA ACAAGGCTCA GCCAGCTAGA ACAGTCAACT 
CTACAAACAG AACTTGAGAA AGAAAAACAA GCCCTCAAGA ACGCCCTGGG AAAAGCCCAG 
CTCTCGGAAG AAAAAGAACA AGAAAACAGT GAGCTCCGCA CCCAGCTTAA ACAGCTACAG 
GATGACAATA ACCTGTTAAA ACAGCAACTT AAAGATTTCC AGAATCACCT TAATCAGGTA 
GCTGATGGTC TGATTCGTCC AGAAGAAGTG GCAGCTCGTG TAGATGAAGT AAGGAGAAAA 
TTGAAATCAG GCGCTGGAGA GATGAGCAGA ATCCACAGTC CTTCAGATGT CTTAGGGAAA 
AGTCTTGCTG ACTTGCAGAT ACAATTCAGT GAAATGCTGA CACGTTCTCA GTGGGAGAAA 
GAGGAAGCCC AAGGGAGGGA AAGAAAACTC CAAGAAGAAA TGGCTCTGCA TCAGGAGAAG 
CTGGTGGATG GACAGGAAGC GTTCAGGCAG GCCTGTGAGA GAGCGCTAGA AGCAAGGATT 
AATTTTGACA AGAGGCAACA TGAAGCAAGA ATTCAGCAGT TAGAGAATGA GATTCACTAT 
TTACAAGAAA ATCTAAAAAG TATGGAGGAA ATCCAAGGGC TTACAGATCT GCAGCTGCAG 
GAAGCTGATG AAGAGAAGGA GAGAATTCTA GCCCAACTCC AGGAGTTAGA GAAGAAGAAG 
AAACTTGAAG ATGCCAAATC TCAGGCACAG GTTCTAGGTT TAGATAAAGA ATTGAAGAAA 
CTAAAGAAGG CTGTGGCCAC CTCTGATAAG ATAGCCACAG CTGAGCTCAC CATCGCCAAA 
GACCAGCTCA AGTCCCTTCA TGGAACTGTT ATGAAAATTA ACCAGGAGCG TGCAGAGGAC 
CTGCAGGAAG CAGAGCGGTT CAGCAGAAAG GCAGCACAGG CCGCGAGGGA TCTGACCCGA 
GCGGAAGCGG AGATCGAACT CCTGCAGAAC CTCCTCAGGG AGAGAGAGGA GCAGATGGAG 
AAGGTAGAGG CAGGCACCCG AGGAGCAAGC TCACAGGCGC TGGAAATCGA GAGGTGGAAT 
GAGACCATGG AGAGACAGAG GAGCGAGATC GCAAGGCTAC GGGACTCACT AGACCGCACC 
GGGGCGGATA ACAGAGGAGG CATTGAAAAT ATTTTGGAAG AAATAGCTGA ACTCCGACGT 
GAAGTTTCTT ACCAGAATGA TTACATCAGC AGCATGGCAG ATCCTTTCAA AAGACGAGGC 
TATTGGTACT TTATGCCACC ACCATCATCA TCAAAAGTTT CCAGCCACAG CTCCCAGGCC 
ACAAAGGACT CTGGTGTCGG GATGAAGTAC GCAGCCTCGA CTCCTATTAG AAAACCACAG 
CAGAACGGGA GGGAAGACGG TGCCCCTCCA GCTGCCTCCG GATACTGGGT GTATTCCCCC 
ATCAGGAGCG GCTTACATAA ATCGTTTTCA AATAAAGATG CAGACAGTGG AGGAGAGAGC 
CGGGAGGAGA GCGAGCTGGA TGACCACGAT GACCGCCCGT TCGTCCCTCC TCCTGGATAC 
ATGATGTACA CCGTGTTCCC CGACGGCTCT CCTGTACCAC AGGGCATGGC CCTCTACGCA 
CCGCCGCCTC CCTTGCCCAA CAATAGCCGA CCTCTCAGCC CTGGCACTGT TGTGTATGGC 
CCGCCCCCTG CTGGTGCCCC CATTGTCTAC GGGCCCCCAC CGCCCAGCTT CTCCGTCCCC 
CTCATCCCAG TGGGCGTGCT GCACTGCAAC GTCCCGGAGC ACCATGCCTT AGAGAATGAG 
GTTTCTAGAT TGGAAGACAT AATACAGCAT TTAAAATCAA AGAAGCAGGA ACAGAGGATG 
AGAGTATGCA AGCGGCAGTC TGAGAAAGAA ATAGAAGAAC TGCATCATAA TATTGATGAA 
CTTTTGCAAG AGAAGAAAGA CTTAGAGCAC GAAGTAGAAG AATTACATAG AACCATCCAG 
AAACATCAAC AGCAAAAAGA CTTCATTGAT GGAAATGTTG AGAGTCTTAT GAATGAACTA 
AAAATAGAAA AATCACTCAA GCACCATGAA GATATTGTAG ATGAAATTGA GTGCATCGAG 
AAGACTCTCC TGAAACGTCG AGCAGAGCTC AGGGAGGCTG ACCGACTGCT GGCGGAAGCC 
GAGAGTGAAC TTTCACGCAC AACAGAGAAA ACAAAAAATG CTGTTGAAAA GTTCACTGAT 
GCCAAGAGGA ATTTGTTGCA AACTGAGAAA GATGCTGAGG AGTTAGAAAG GAGAGCTCAG 
GAAACAGCTG TTAGCCTTGT CAAGGCTGAT CAACAGCTCA GATTGTTACA GGCGGACACA 
AAGGATTTGG AGCAGCACAA AACTGAGCAA GAAGAGATCT TGAAAGAGAT AAACAAAGTT 
GTTGCAGCAA AAGACTCAGA CTTCCAGTGT TTAAACACGA AGAAGGAGAA GCTAACAGAA 
GAGCTGCAGA AGCTGCAGAA AGACATCGAG ACGGCAGCGC GCACGGAGGA TCACCACCTG 
CAGGTCCTGA GAGAGTCGGA GGCCCTCCTG CAGGCCAAGA GAGCGGAGCT GGACAAGCTG 
AGGAGCCAGG TAACGAGTCA GCAGCAGGAG ATGGCTGTCT TGGACAGACA GCTGGGGCAT 
AAGAAGGAGG AGCTGCATCT ACTCCAGGGA AGCATGGTCC AGGCCAGAGC TGACCTCCAG 
GAAGCGCTGA GGCTGGGAGA AACTGAAGTA ACTGAGAAGT GCAACCACAT CAGGGAACTA 
AAAGGTCTTC TGGAAGAACT GAGTTTTCAG AAAGGAGAAC TGAATGTCCA GATTAGTGAG 
AAAAAAAATC AACTTTCACT TATAAAGCAA GAAATTGAAA AAGAGGAAGA AAATCTTCAG 
GTAGTTTTAG GGCAGATGTC TAAACATAAA ACTGAACTTA AGAATATTCT GGACATGTTG 
CAACTTGAAA ACAATGAGCT GCAAGCTTTG AAGCTACAGC ATGAGCAAAA GATGTCTGAG 
TTAGAGAAGG CTCAGGTTGA AGTGCTGGAG GAGAAACTGG AGTTGGAGAA TTTGCAGCAG 
ACAGCCCTGC GACAGAAAGG GGAGATCGAG TGGCAGAAGC AGCTCTTAGA GAGGGACAAG 
CGAGAGATTG AGCGGATAAC TGCTGAGACC CGAGCATTGC AGTTGTGTGT TGAGTCTTTG 
TGCAAACAAA AGGAAGATCT TGAGGAGAAA TGTGACAGCT GGGAGAAGAA GCTGGCACAG 
ACCAAACGGG TTTTAGCAGC CACAGAAGAG AATAGCAAAG CAAAGCAATC AGACTTAGAA 
AAGTTGGAAT TGGATATCAG AAAACTGGAA CAGGAACTAG ACCAGCTAAA CAGAAACAAA 
CTCTCACTGC ACAGAGACAT CGCGGCCATG CAGCAGCAGG TCCAAGAAAA ACGAGAAGCA 
GTAAACTCAT TACAGGAGGA ACTAGCTGAT GTCCAAGCCC ATTTGAACCT AGCGAAACAG 
GACCTGCTCC ACACCACCAA GCGCCAGGAT GTCCTGCTTG GCGAGCAGAC CAGGCTGCAG 
GAGGACCTCC AGGAACGCAG AAAGAAGTTG GAAGTCTGCC AAAAAGAAGA GGCAACAAAG 
CGGCAGCACC TGCAAGCGCT TCAGACGGAG ATGGAGGCGA ACGAGGCCGA GCTGGCCCGG 
CAGGAGACGG TACCGCACCC GCGGGCACGC GCGGGGGGGC ACTGCCACGC GCAGAGCGCT 
CTTCCCCTGG GGTCTGAGGT GGAGGCGAAC GAGAGCGAGC TGGCCCGGCA GGAGACGACA 
TTTCAGAGAC TCCAGAAAGA GAGAGAAAGT GAAGAAAAAA AGTTAGAAGC CAGTCGAGCG 
ACTCTGCAGG AGCAGCAGCA GCAGCTGGAA AAGGAAATCG CAGACCAGAA GAACGTGCTG 
GACCAGGTGC TCAGCCAGGT GCTGGGGGCC AAGGAGCGCG CCAGGACTCT GCAGCAGGAG 
GAGAGGTGGG GTGAGACCCT GGAGAAGACG CTCTCCCAGA CCCAGCAACA GCTCTCAGAA 
CGGGAGCAGC AGTTGTTGGA AAAGTCAGCT GAGCTGCTGG CTCTTCAGAA AGAGGCAGAC 
TCGATGAGGG CAGACTTCAG CCTCTTGCGA AACCAGTTCT TGACGGAGAG GAAGAAAGCC 
GAGAAGCAGG TAGCCAGCCT CAGGGAAGCG CTCAAGACCC AGCGCAGCCA GCTGGAGAAG 
ACCCTTCTCG AGCAGAAGCA GGAGAACAGC TCCATGCAGA AGGAGATGGC GACTATCGAG 
CAGGTGGCGC AGGACAACCA CGAGCGGGCC CGGCGCCTGA TGCGGGAGCT CAGCCAGATG 
CAGCAGGAGT ACGCGGAGCT CAAGAAGCAG ATGGTAAACC AGAAAGATCT GGAAAGAAGA 
CAAGTAGAAA TCAGTGAAGC GATGAGGACA CTTAAGTCTG AAGTGAAAGA CGAGATCAGA 
GCCAGCCTGA AGAACCTCAA CCAGTTTCTC CCGGAGCTGC CAGCAGACCT GGAGGCCATT 
CTGGAAAGGA GCGAACACCT AGACGGAGGG CTGGAGAGCT TGAAGGAGAA CTTTCCATTC 
GCCCTGAGTG AGAAGCCATC ACCTTTGGAA GAGAAACTAC ATTTTTCTCG CGTTCACATA 
ATGGCCCAGC TCCGACACTG TATGTCCAAG CAGGCAGAGG TGTTAAGCAA AGGGAAGCGG 
CAGACCGAGG GCACGTTACA CAGCCTGAGG AGGCAGGTCG ACGCCTTAGG GGAGCTGGTC 
ACCAGCACGT CTGTGGAGTC CACGTCGTCA GCCACTCTGT CTCAGCTCGA ATCTTCCCTT 
GCAGAGGACT CCCATCTTGG ACCAAGTCAG AGCTCCTTCC ATCAAGTTCC ACAAGGTCCA 
TCACAATCAG TAGACAGTTA CCGACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025138134.1
CDS	361..7470
Translation	MKKGPQQKISKTKMPPSSHSPSPSSLTSNMRSRSLSPLNGSETLPFHSGRQWYGQVEIIGENSMLLDYHDQKEADSHAGVRYITEALVKKLTKQENLTLVKSLNLSLSKDGGKKFKYIENLEKCTKLEILNLSHNLIGKIEKVDKLLKLRELNLSYNKICKIEGIENMHNLQKLNLAGNEIEHIPAWLGKKLKCLRVLNLKANKISSLQDVSKLKPLQDLTSLILAENPVVTLPHYLQFTIFHLRSLESLEGQPVTTQDRQEAFERFSLEEVERLERDLEKKMMETEELKSKQAQFLEEIKNQDKLNRSLKEEAMLQKQSCEELESNLNTKNELPLSRFLADPCLPILYSSLKLRQKTMELTRACQKQYELEQELAFYKIDAKFEPLNYYPSEYVDVDKAPDESPYIGKSRYKRNMFATESYIVNNAQPVEIKRMEPEEGEQLRNEHVSLKAHMPLDTELEDKENKISTAQARLSALHDEIEKAEQQILRATEEFKQLEEAVQLKKISEAEKDFLLKQLSGRIQLLNKLRQEALDLEMQMEKQRGEIAEKQEEIKDLQRVIDSLDSKDPKHCHMKAQKRGKEQQFDIMNKQYKQLESRLDEILSRIAKETEEIKDLEQQLTEGQIAANEALKKDLEGVISGLQEYLGTVKGQAAQAQSECRRLQDEKEALLQRLAEVEQERDQLEAVALDAEAMRKELAELESALQEQHEVNASLQQTHGDLSAYEAELEAQLRMRDAEASQLKEELGELTRLSQLEQSTLQTELEKEKQALKNALGKAQLSEEKEQENSELRTQLKQLQDDNNLLKQQLKDFQNHLNQVADGLIRPEEVAARVDEVRRKLKSGAGEMSRIHSPSDVLGKSLADLQIQFSEMLTRSQWEKEEAQGRERKLQEEMALHQEKLVDGQEAFRQACERALEARINFDKRQHEARIQQLENEIHYLQENLKSMEEIQGLTDLQLQEADEEKERILAQLQELEKKKKLEDAKSQAQVLGLDKELKKLKKAVATSDKIATAELTIAKDQLKSLHGTVMKINQERAEDLQEAERFSRKAAQAARDLTRAEAEIELLQNLLREREEQMEKVEAGTRGASSQALEIERWNETMERQRSEIARLRDSLDRTGADNRGGIENILEEIAELRREVSYQNDYISSMADPFKRRGYWYFMPPPSSSKVSSHSSQATKDSGVGMKYAASTPIRKPQQNGREDGAPPAASGYWVYSPIRSGLHKSFSNKDADSGGESREESELDDHDDRPFVPPPGYMMYTVFPDGSPVPQGMALYAPPPPLPNNSRPLSPGTVVYGPPPAGAPIVYGPPPPSFSVPLIPVGVLHCNVPEHHALENEVSRLEDIIQHLKSKKQEQRMRVCKRQSEKEIEELHHNIDELLQEKKDLEHEVEELHRTIQKHQQQKDFIDGNVESLMNELKIEKSLKHHEDIVDEIECIEKTLLKRRAELREADRLLAEAESELSRTTEKTKNAVEKFTDAKRNLLQTEKDAEELERRAQETAVSLVKADQQLRLLQADTKDLEQHKTEQEEILKEINKVVAAKDSDFQCLNTKKEKLTEELQKLQKDIETAARTEDHHLQVLRESEALLQAKRAELDKLRSQVTSQQQEMAVLDRQLGHKKEELHLLQGSMVQARADLQEALRLGETEVTEKCNHIRELKGLLEELSFQKGELNVQISEKKNQLSLIKQEIEKEEENLQVVLGQMSKHKTELKNILDMLQLENNELQALKLQHEQKMSELEKAQVEVLEEKLELENLQQTALRQKGEIEWQKQLLERDKREIERITAETRALQLCVESLCKQKEDLEEKCDSWEKKLAQTKRVLAATEENSKAKQSDLEKLELDIRKLEQELDQLNRNKLSLHRDIAAMQQQVQEKREAVNSLQEELADVQAHLNLAKQDLLHTTKRQDVLLGEQTRLQEDLQERRKKLEVCQKEEATKRQHLQALQTEMEANEAELARQETVPHPRARAGGHCHAQSALPLGSEVEANESELARQETTFQRLQKERESEEKKLEASRATLQEQQQQLEKEIADQKNVLDQVLSQVLGAKERARTLQQEERWGETLEKTLSQTQQQLSEREQQLLEKSAELLALQKEADSMRADFSLLRNQFLTERKKAEKQVASLREALKTQRSQLEKTLLEQKQENSSMQKEMATIEQVAQDNHERARRLMRELSQMQQEYAELKKQMVNQKDLERRQVEISEAMRTLKSEVKDEIRASLKNLNQFLPELPADLEAILERSEHLDGGLESLKENFPFALSEKPSPLEEKLHFSRVHIMAQLRHCMSKQAEVLSKGKRQTEGTLHSLRRQVDALGELVTSTSVESTSSATLSQLESSLAEDSHLGPSQSSFHQVPQGPSQSVDSYRH

Target ORF information:

RefSeq Version	XM_025282349.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centriolin (CNTRL), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025282349.1

ORF Insert Sequence:

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCCCTTGTC TCGGTTTCTT 
GCGGACCCTT GTCTGCCGAT TCTTTATTCT TCCTTAAAGC TAAGACAGAA GACCATGGAA 
CTAACGCGAG CATGTCAAAA ACAGTATGAA CTGGAACAGG AGTTGGCTTT TTACAAAATC 
GATGCTAAAT TTGAGCCACT GAATTATTAT CCCTCAGAGT ATGTTGACGT TGATAAAGCC 
CCAGATGAAA GCCCTTACAT TGGCAAGTCC AGATACAAGA GAAATATGTT CGCCACAGAG 
AGTTACATTG TGAATAATGC CCAGCCAGTT GAGATCAAGA GGATGGAGCC AGAGGAAGGG 
GAGCAACTTA GAAACGAGCA TGTGAGCTTG AAAGCCCATA TGCCACTGGA CACGGAGCTG 
GAAGACAAAG AGAACAAAAT AAGTACAGCA CAAGCTCGAC TATCAGCACT GCATGATGAA 
ATAGAAAAGG CAGAACAACA AATTTTAAGA GCCACTGAAG AATTTAAACA ACTGGAAGAA 
GCTGTACAAC TTAAAAAGAT TTCAGAAGCA GAGAAAGACT TTCTTCTCAA GCAGCTGAGT 
GGCAGGATAC AGCTTCTAAA TAAATTACGC CAGGAAGCTC TTGATCTAGA AATGCAGATG 
GAAAAGCAAA GAGGAGAAAT TGCTGAAAAG CAGGAGGAAA TCAAGGACCT CCAAAGAGTT 
ATTGATAGTC TGGATTCCAA AGACCCAAAA CATTGCCATA TGAAGGCTCA GAAAAGGGGT 
AAAGAACAAC AATTTGACAT TATGAACAAG CAGTACAAAC AACTTGAGAG CCGTTTGGAC 
GAGATACTTT CTAGAATTGC TAAAGAAACT GAAGAGATTA AGGACCTTGA ACAACAGCTT 
ACTGAAGGCC AGATAGCAGC AAATGAAGCC CTGAAGAAGG ACTTAGAAGG CGTCATCAGT 
GGCCTGCAAG AATACCTGGG CACTGTGAAG GGCCAGGCCG CTCAGGCCCA GAGCGAGTGC 
AGAAGGCTGC AGGATGAGAA AGAGGCGCTG CTGCAGAGGT TGGCCGAGGT CGAGCAGGAA 
AGGGACCAGC TGGAAGCAGT CGCCTTAGAT GCAGAAGCTA TGAGGAAGGA GCTTGCAGAG 
CTAGAAAGCG CCCTCCAAGA GCAGCATGAG GTGAACGCGT CCCTGCAGCA GACCCACGGA 
GACCTCAGTG CCTACGAGGC TGAGCTAGAG GCACAGCTGA GGATGAGGGA TGCCGAAGCC 
AGCCAGCTCA AAGAAGAGCT GGGAGAACTA ACAAGGCTCA GCCAGCTAGA ACAGTCAACT 
CTACAAACAG AACTTGAGAA AGAAAAACAA GCCCTCAAGA ACGCCCTGGG AAAAGCCCAG 
CTCTCGGAAG AAAAAGAACA AGAAAACAGT GAGCTCCGCA CCCAGCTTAA ACAGCTACAG 
GATGACAATA ACCTGTTAAA ACAGCAACTT AAAGATTTCC AGAATCACCT TAATCAGGTA 
GCTGATGGTC TGATTCGTCC AGAAGAAGTG GCAGCTCGTG TAGATGAAGT AAGGAGAAAA 
TTGAAATCAG GCGCTGGAGA GATGAGCAGA ATCCACAGTC CTTCAGATGT CTTAGGGAAA 
AGTCTTGCTG ACTTGCAGAT ACAATTCAGT GAAATGCTGA CACGTTCTCA GTGGGAGAAA 
GAGGAAGCCC AAGGGAGGGA AAGAAAACTC CAAGAAGAAA TGGCTCTGCA TCAGGAGAAG 
CTGGTGGATG GACAGGAAGC GTTCAGGCAG GCCTGTGAGA GAGCGCTAGA AGCAAGGATT 
AATTTTGACA AGAGGCAACA TGAAGCAAGA ATTCAGCAGT TAGAGAATGA GATTCACTAT 
TTACAAGAAA ATCTAAAAAG TATGGAGGAA ATCCAAGGGC TTACAGATCT GCAGCTGCAG 
GAAGCTGATG AAGAGAAGGA GAGAATTCTA GCCCAACTCC AGGAGTTAGA GAAGAAGAAG 
AAACTTGAAG ATGCCAAATC TCAGGCACAG GTTCTAGGTT TAGATAAAGA ATTGAAGAAA 
CTAAAGAAGG CTGTGGCCAC CTCTGATAAG ATAGCCACAG CTGAGCTCAC CATCGCCAAA 
GACCAGCTCA AGTCCCTTCA TGGAACTGTT ATGAAAATTA ACCAGGAGCG TGCAGAGGAC 
CTGCAGGAAG CAGAGCGGTT CAGCAGAAAG GCAGCACAGG CCGCGAGGGA TCTGACCCGA 
GCGGAAGCGG AGATCGAACT CCTGCAGAAC CTCCTCAGGG AGAGAGAGGA GCAGATGGAG 
AAGGTAGAGG CAGGCACCCG AGGAGCAAGC TCACAGGCGC TGGAAATCGA GAGGTGGAAT 
GAGACCATGG AGAGACAGAG GAGCGAGATC GCAAGGCTAC GGGACTCACT AGACCGCACC 
GGGGCGGATA ACAGAGGAGG CATTGAAAAT ATTTTGGAAG AAATAGCTGA ACTCCGACGT 
GAAGTTTCTT ACCAGAATGA TTACATCAGC AGCATGGCAG ATCCTTTCAA AAGACGAGGC 
TATTGGTACT TTATGCCACC ACCATCATCA TCAAAAGTTT CCAGCCACAG CTCCCAGGCC 
ACAAAGGACT CTGGTGTCGG GATGAAGTAC GCAGCCTCGA CTCCTATTAG AAAACCACAG 
CAGAACGGGA GGGAAGACGG TGCCCCTCCA GCTGCCTCCG GATACTGGGT GTATTCCCCC 
ATCAGGAGCG GCTTACATAA ATCGTTTTCA AATAAAGATG CAGACAGTGG AGGAGAGAGC 
CGGGAGGAGA GCGAGCTGGA TGACCACGAT GACCGCCCGT TCGTCCCTCC TCCTGGATAC 
ATGATGTACA CCGTGTTCCC CGACGGCTCT CCTGTACCAC AGGGCATGGC CCTCTACGCA 
CCGCCGCCTC CCTTGCCCAA CAATAGCCGA CCTCTCAGCC CTGGCACTGT TGTGTATGGC 
CCGCCCCCTG CTGGTGCCCC CATTGTCTAC GGGCCCCCAC CGCCCAGCTT CTCCGTCCCC 
CTCATCCCAG TGGGCGTGCT GCACTGCAAC GTCCCGGAGC ACCATGCCTT AGAGAATGAG 
GTTTCTAGAT TGGAAGACAT AATACAGCAT TTAAAATCAA AGAAGCAGGA ACAGAGGATG 
AGAGTATGCA AGCGGCAGTC TGAGAAAGAA ATAGAAGAAC TGCATCATAA TATTGATGAA 
CTTTTGCAAG AGAAGAAAGA CTTAGAGCAC GAAGTAGAAG AATTACATAG AACCATCCAG 
AAACATCAAC AGCAAAAAGA CTTCATTGAT GGAAATGTTG AGAGTCTTAT GAATGAACTA 
AAAATAGAAA AATCACTCAA GCACCATGAA GATATTGTAG ATGAAATTGA GTGCATCGAG 
AAGACTCTCC TGAAACGTCG AGCAGAGCTC AGGGAGGCTG ACCGACTGCT GGCGGAAGCC 
GAGAGTGAAC TTTCACGCAC AACAGAGAAA ACAAAAAATG CTGTTGAAAA GTTCACTGAT 
GCCAAGAGGA ATTTGTTGCA AACTGAGAAA GATGCTGAGG AGTTAGAAAG GAGAGCTCAG 
GAAACAGCTG TTAGCCTTGT CAAGGCTGAT CAACAGCTCA GATTGTTACA GGCGGACACA 
AAGGATTTGG AGCAGCACAA AACTGAGCAA GAAGAGATCT TGAAAGAGAT AAACAAAGTT 
GTTGCAGCAA AAGACTCAGA CTTCCAGTGT TTAAACACGA AGAAGGAGAA GCTAACAGAA 
GAGCTGCAGA AGCTGCAGAA AGACATCGAG ACGGCAGCGC GCACGGAGGA TCACCACCTG 
CAGGTCCTGA GAGAGTCGGA GGCCCTCCTG CAGGCCAAGA GAGCGGAGCT GGACAAGCTG 
AGGAGCCAGG TAACGAGTCA GCAGCAGGAG ATGGCTGTCT TGGACAGACA GCTGGGGCAT 
AAGAAGGAGG AGCTGCATCT ACTCCAGGGA AGCATGGTCC AGGCCAGAGC TGACCTCCAG 
GAAGCGCTGA GGCTGGGAGA AACTGAAGTA ACTGAGAAGT GCAACCACAT CAGGGAACTA 
AAAGGTCTTC TGGAAGAACT GAGTTTTCAG AAAGGAGAAC TGAATGTCCA GATTAGTGAG 
AAAAAAAATC AACTTTCACT TATAAAGCAA GAAATTGAAA AAGAGGAAGA AAATCTTCAG 
GTAGTTTTAG GGCAGATGTC TAAACATAAA ACTGAACTTA AGAATATTCT GGACATGTTG 
CAACTTGAAA ACAATGAGCT GCAAGCTTTG AAGCTACAGC ATGAGCAAAA GATGTCTGAG 
TTAGAGAAGG CTCAGGTTGA AGTGCTGGAG GAGAAACTGG AGTTGGAGAA TTTGCAGCAG 
ACAGCCCTGC GACAGAAAGG GGAGATCGAG TGGCAGAAGC AGCTCTTAGA GAGGGACAAG 
CGAGAGATTG AGCGGATAAC TGCTGAGACC CGAGCATTGC AGTTGTGTGT TGAGTCTTTG 
TGCAAACAAA AGGAAGATCT TGAGGAGAAA TGTGACAGCT GGGAGAAGAA GCTGGCACAG 
ACCAAACGGG TTTTAGCAGC CACAGAAGAG AATAGCAAAG CAAAGCAATC AGACTTAGAA 
AAGTTGGAAT TGGATATCAG AAAACTGGAA CAGGAACTAG ACCAGCTAAA CAGAAACAAA 
CTCTCACTGC ACAGAGACAT CGCGGCCATG CAGCAGCAGG TCCAAGAAAA ACGAGAAGCA 
GTAAACTCAT TACAGGAGGA ACTAGCTGAT GTCCAAGCCC ATTTGAACCT AGCGAAACAG 
GACCTGCTCC ACACCACCAA GCGCCAGGAT GTCCTGCTTG GCGAGCAGAC CAGGCTGCAG 
GAGGACCTCC AGGAACGCAG AAAGAAGTTG GAAGTCTGCC AAAAAGAAGA GGCAACAAAG 
CGGCAGCACC TGCAAGCGCT TCAGACGGAG ATGGAGGCGA ACGAGGCCGA GCTGGCCCGG 
CAGGAGACGG TACCGCACCC GCGGGCACGC GCGGGGGGGC ACTGCCACGC GCAGAGCGCT 
CTTCCCCTGG GGTCTGAGGT GGAGGCGAAC GAGAGCGAGC TGGCCCGGCA GGAGACGACA 
TTTCAGAGAC TCCAGAAAGA GAGAGAAAGT GAAGAAAAAA AGTTAGAAGC CAGTCGAGCG 
ACTCTGCAGG AGCAGCAGCA GCAGCTGGAA AAGGAAATCG CAGACCAGAA GAACGTGCTG 
GACCAGGTGC TCAGCCAGGT GCTGGGGGCC AAGGAGCGCG CCAGGACTCT GCAGCAGGAG 
GAGAGGTGGG GTGAGACCCT GGAGAAGACG CTCTCCCAGA CCCAGCAACA GCTCTCAGAA 
CGGGAGCAGC AGTTGTTGGA AAAGTCAGCT GAGCTGCTGG CTCTTCAGAA AGAGGCAGAC 
TCGATGAGGG CAGACTTCAG CCTCTTGCGA AACCAGTTCT TGACGGAGAG GAAGAAAGCC 
GAGAAGCAGG TAGCCAGCCT CAGGGAAGCG CTCAAGACCC AGCGCAGCCA GCTGGAGAAG 
ACCCTTCTCG AGCAGAAGCA GGAGAACAGC TCCATGCAGA AGGAGATGGC GACTATCGAG 
CAGGTGGCGC AGGACAACCA CGAGCGGGCC CGGCGCCTGA TGCGGGAGCT CAGCCAGATG 
CAGCAGGAGT ACGCGGAGCT CAAGAAGCAG ATGGTAAACC AGAAAGATCT GGAAAGAAGA 
CAAGTAGAAA TCAGTGAAGC GATGAGGACA CTTAAGTCTG AAGTGAAAGA CGAGATCAGA 
GCCAGCCTGA AGAACCTCAA CCAGTTTCTC CCGGAGCTGC CAGCAGACCT GGAGGCCATT 
CTGGAAAGGA GCGAACACCT AGACGGAGGG CTGGAGAGCT TGAAGGAGAA CTTTCCATTC 
GCCCTGAGTG AGAAGCCATC ACCTTTGGAA GAGAAACTAC ATTTTTCTCG CGTTCACATA 
ATGGCCCAGC TCCGACACTG TATGTCCAAG CAGGCAGAGG TGTTAAGCAA AGGGAAGCGG 
CAGACCGAGG GCACGTTACA CAGCCTGAGG AGGCAGGTCG ACGCCTTAGG GGAGCTGGTC 
ACCAGCACGT CTGTGGAGTC CACGTCGTCA GCCACTCTGT CTCAGCTCGA ATCTTCCCTT 
GCAGAGGACT CCCATCTTGG ACCAAGTCAG AGCTCCTTCC ATCAAGTTCC ACAAGGTCCA 
TCACAATCAG TAGACAGTTA CCGACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTRL ( XM_025282351.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025138136.1 Bubalus bubalis centriolin (CNTRL), transcript variant X8, mRNA.

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CloneID	OBu63438
Clone ID Related Accession (Same CDS sequence)	XM_025282351.1
Accession Version	XM_025282351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	centriolin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCCCTTGTC TCGGTTTCTT 
GCGGACCCTT GTCTGCCGAT TCTTTATTCT TCCTTAAAGC TAAGACAGAA GACCATGGAA 
CTAACGCGAG CATGTCAAAA ACAGTATGAA CTGGAACAGG AGTTGGCTTT TTACAAAATC 
GATGCTAAAT TTGAGCCACT GAATTATTAT CCCTCAGAGT ATGTTGACGT TGATAAAGCC 
CCAGATGAAA GCCCTTACAT TGGCAAGTCC AGATACAAGA GAAATATGTT CGCCACAGAG 
AGTTACATTG TGAATAATGC CCAGCCAGTT GAGATCAAGA GGATGGAGCC AGAGGAAGGG 
GAGCAACTTA GAAACGAGCA TGTGAGCTTG AAAGCCCATA TGCCACTGGA CACGGAGCTG 
GAAGACAAAG AGAACAAAAT AAGTACAGCA CAAGCTCGAC TATCAGCACT GCATGATGAA 
ATAGAAAAGG CAGAACAACA AATTTTAAGA GCCACTGAAG AATTTAAACA ACTGGAAGAA 
GCTGTACAAC TTAAAAAGAT TTCAGAAGCA GAGAAAGACT TTCTTCTCAA GCAGCTGAGT 
GGCAGGATAC AGCTTCTAAA TAAATTACGC CAGGAAGCTC TTGATCTAGA AATGCAGATG 
GAAAAGCAAA GAGGAGAAAT TGCTGAAAAG CAGGAGGAAA TCAAGGACCT CCAAAGAGTT 
ATTGATAGTC TGGATTCCAA AGACCCAAAA CATTGCCATA TGAAGGCTCA GAAAAGGGGT 
AAAGAACAAC AATTTGACAT TATGAACAAG CAGTACAAAC AACTTGAGAG CCGTTTGGAC 
GAGATACTTT CTAGAATTGC TAAAGAAACT GAAGAGATTA AGGACCTTGA ACAACAGCTT 
ACTGAAGGCC AGATAGCAGC AAATGAAGCC CTGAAGAAGG ACTTAGAAGG CGTCATCAGT 
GGCCTGCAAG AATACCTGGG CACTGTGAAG GGCCAGGCCG CTCAGGCCCA GAGCGAGTGC 
AGAAGGCTGC AGGATGAGAA AGAGGCGCTG CTGCAGAGGT TGGCCGAGGT CGAGCAGGAA 
AGGGACCAGC TGGAAGCAGT CGCCTTAGAT GCAGAAGCTA TGAGGAAGGA GCTTGCAGAG 
CTAGAAAGCG CCCTCCAAGA GCAGCATGAG GTGAACGCGT CCCTGCAGCA GACCCACGGA 
GACCTCAGTG CCTACGAGGC TGAGCTAGAG GCACAGCTGA GGATGAGGGA TGCCGAAGCC 
AGCCAGCTCA AAGAAGAGCT GGGAGAACTA ACAAGGCTCA GCCAGCTAGA ACAGTCAACT 
CTACAAACAG AACTTGAGAA AGAAAAACAA GCCCTCAAGA ACGCCCTGGG AAAAGCCCAG 
CTCTCGGAAG AAAAAGAACA AGAAAACAGT GAGCTCCGCA CCCAGCTTAA ACAGCTACAG 
GATGACAATA ACCTGTTAAA ACAGCAACTT AAAGATTTCC AGAATCACCT TAATCAGGTA 
GCTGATGGTC TGATTCGTCC AGAAGAAGTG GCAGCTCGTG TAGATGAAGT AAGGAGAAAA 
TTGAAATCAG GCGCTGGAGA GATGAGCAGA ATCCACAGTC CTTCAGATGT CTTAGGGAAA 
AGTCTTGCTG ACTTGCAGAT ACAATTCAGT GAAATGCTGA CACGTTCTCA GTGGGAGAAA 
GAGGAAGCCC AAGGGAGGGA AAGAAAACTC CAAGAAGAAA TGGCTCTGCA TCAGGAGAAG 
CTGGTGGATG GACAGGAAGC GTTCAGGCAG GCCTGTGAGA GAGCGCTAGA AGCAAGGATT 
AATTTTGACA AGAGGCAACA TGAAGCAAGA ATTCAGCAGT TAGAGAATGA GATTCACTAT 
TTACAAGAAA ATCTAAAAAG TATGGAGGAA ATCCAAGGGC TTACAGATCT GCAGCTGCAG 
GAAGCTGATG AAGAGAAGGA GAGAATTCTA GCCCAACTCC AGGAGTTAGA GAAGAAGAAG 
AAACTTGAAG ATGCCAAATC TCAGGCACAG GTTCTAGGTT TAGATAAAGA ATTGAAGAAA 
CTAAAGAAGG CTGTGGCCAC CTCTGATAAG ATAGCCACAG CTGAGCTCAC CATCGCCAAA 
GACCAGCTCA AGTCCCTTCA TGGAACTGTT ATGAAAATTA ACCAGGAGCG TGCAGAGGAC 
CTGCAGGAAG CAGAGCGGTT CAGCAGAAAG GCAGCACAGG CCGCGAGGGA TCTGACCCGA 
GCGGAAGCGG AGATCGAACT CCTGCAGAAC CTCCTCAGGG AGAGAGAGGA GCAGATGGAG 
AAGGTAGAGG CAGGCACCCG AGGAGCAAGC TCACAGGCGC TGGAAATCGA GAGGTGGAAT 
GAGACCATGG AGAGACAGAG GAGCGAGATC GCAAGGCTAC GGGACTCACT AGACCGCACC 
GGGGCGGATA ACAGAGGAGG CATTGAAAAT ATTTTGGAAG AAATAGCTGA ACTCCGACGT 
GAAGTTTCTT ACCAGAATGA TTACATCAGC AGCATGGCAG ATCCTTTCAA AAGACGAGGC 
TATTGGTACT TTATGCCACC ACCATCATCA TCAAAAGTTT CCAGCCACAG CTCCCAGGCC 
ACAAAGGACT CTGGTGTCGG GATGAAGTAC GCAGCCTCGA CTCCTATTAG AAAACCACAG 
CAGAACGGGA GGGAAGACGG TGCCCCTCCA GCTGCCTCCG GATACTGGGT GTATTCCCCC 
ATCAGGAGCG GCTTACATAA ATCGTTTTCA AATAAAGATG CAGACAGTGG AGGAGAGAGC 
CGGGAGGAGA GCGAGCTGGA TGACCACGAT GACCGCCCGT TCGTCCCTCC TCCTGGATAC 
ATGATGTACA CCGTGTTCCC CGACGGCTCT CCTGTACCAC AGGGCATGGC CCTCTACGCA 
CCGCCGCCTC CCTTGCCCAA CAATAGCCGA CCTCTCAGCC CTGGCACTGT TGTGTATGGC 
CCGCCCCCTG CTGGTGCCCC CATTGTCTAC GGGCCCCCAC CGCCCAGCTT CTCCGTCCCC 
CTCATCCCAG TGGGCGTGCT GCACTGCAAC GTCCCGGAGC ACCATGCCTT AGAGAATGAG 
GTTTCTAGAT TGGAAGACAT AATACAGCAT TTAAAATCAA AGAAGCAGGA ACAGAGGATG 
AGAGTATGCA AGCGGCAGTC TGAGAAAGAA ATAGAAGAAC TGCATCATAA TATTGATGAA 
CTTTTGCAAG AGAAGAAAGA CTTAGAGCAC GAAGTAGAAG AATTACATAG AACCATCCAG 
AAACATCAAC AGCAAAAAGA CTTCATTGAT GGAAATGTTG AGAGTCTTAT GAATGAACTA 
AAAATAGAAA AATCACTCAA GCACCATGAA GATATTGTAG ATGAAATTGA GTGCATCGAG 
AAGACTCTCC TGAAACGTCG AGCAGAGCTC AGGGAGGCTG ACCGACTGCT GGCGGAAGCC 
GAGAGTGAAC TTTCACGCAC AACAGAGAAA ACAAAAAATG CTGTTGAAAA GTTCACTGAT 
GCCAAGAGGA ATTTGTTGCA AACTGAGAAA GATGCTGAGG AGTTAGAAAG GAGAGCTCAG 
GAAACAGCTG TTAGCCTTGT CAAGGCTGAT CAACAGCTCA GATTGTTACA GGCGGACACA 
AAGGATTTGG AGCAGCACAA AACTGAGCAA GAAGAGATCT TGAAAGAGAT AAACAAAGTT 
GTTGCAGCAA AAGACTCAGA CTTCCAGTGT TTAAACACGA AGAAGGAGAA GCTAACAGAA 
GAGCTGCAGA AGCTGCAGAA AGACATCGAG ACGGCAGCGC GCACGGAGGA TCACCACCTG 
CAGGTCCTGA GAGAGTCGGA GGCCCTCCTG CAGGCCAAGA GAGCGGAGCT GGACAAGCTG 
AGGAGCCAGG TAACGAGTCA GCAGCAGGAG ATGGCTGTCT TGGACAGACA GCTGGGGCAT 
AAGAAGGAGG AGCTGCATCT ACTCCAGGGA AGCATGGTCC AGGCCAGAGC TGACCTCCAG 
GAAGCGCTGA GGCTGGGAGA AACTGAAGTA ACTGAGAAGT GCAACCACAT CAGGGAACTA 
AAAGGTCTTC TGGAAGAACT GAGTTTTCAG AAAGGAGAAC TGAATGTCCA GATTAGTGAG 
AAAAAAAATC AACTTTCACT TATAAAGCAA GAAATTGAAA AAGAGGAAGA AAATCTTCAG 
GTAGTTTTAG GGCAGATGTC TAAACATAAA ACTGAACTTA AGAATATTCT GGACATGTTG 
CAACTTGAAA ACAATGAGCT GCAAGCTTTG AAGCTACAGC ATGAGCAAAA GATGTCTGAG 
TTAGAGAAGG CTCAGGTTGA AGTGCTGGAG GAGAAACTGG AGTTGGAGAA TTTGCAGCAG 
ACAGCCCTGC GACAGAAAGG GGAGATCGAG TGGCAGAAGC AGCTCTTAGA GAGGGACAAG 
CGAGAGATTG AGCGGATAAC TGCTGAGACC CGAGCATTGC AGTTGTGTGT TGAGTCTTTG 
TGCAAACAAA AGGAAGATCT TGAGGAGAAA TGTGACAGCT GGGAGAAGAA GCTGGCACAG 
ACCAAACGGG TTTTAGCAGC CACAGAAGAG AATAGCAAAG CAAAGCAATC AGACTTAGAA 
AAGTTGGAAT TGGATATCAG AAAACTGGAA CAGGAACTAG ACCAGCTAAA CAGAAACAAA 
CTCTCACTGC ACAGAGACAT CGCGGCCATG CAGCAGCAGG TCCAAGAAAA ACGAGAAGCA 
GTAAACTCAT TACAGGAGGA ACTAGCTGAT GTCCAAGCCC ATTTGAACCT AGCGAAACAG 
GACCTGCTCC ACACCACCAA GCGCCAGGAT GTCCTGCTTG GCGAGCAGAC CAGGCTGCAG 
GAGGACCTCC AGGAACGCAG AAAGAAGTTG GAAGTCTGCC AAAAAGAAGA GGCAACAAAG 
CGGCAGCACC TGCAAGCGCT TCAGACGGAG ATGGAGGCGA ACGAGGCCGA GCTGGCCCGG 
CAGGAGACGA CATTTCAGAG ACTCCAGAAA GAGAGAGAAA GTGAAGAAAA AAAGTTAGAA 
GCCAGTCGAG CGACTCTGCA GGAGCAGCAG CAGCAGCTGG AAAAGGAAAT CGCAGACCAG 
AAGAACGTGC TGGACCAGGT GCTCAGCCAG GTGCTGGGGG CCAAGGAGCG CGCCAGGACT 
CTGCAGCAGG AGGAGAGGTG GGGTGAGACC CTGGAGAAGA CGCTCTCCCA GACCCAGCAA 
CAGCTCTCAG AACGGGAGCA GCAGTTGTTG GAAAAGTCAG CTGAGCTGCT GGCTCTTCAG 
AAAGAGGCAG ACTCGATGAG GGCAGACTTC AGCCTCTTGC GAAACCAGTT CTTGACGGAG 
AGGAAGAAAG CCGAGAAGCA GGTAGCCAGC CTCAGGGAAG CGCTCAAGAC CCAGCGCAGC 
CAGCTGGAGA AGACCCTTCT CGAGCAGAAG CAGGAGAACA GCTCCATGCA GAAGGAGATG 
GCGACTATCG AGCAGGTGGC GCAGGACAAC CACGAGCGGG CCCGGCGCCT GATGCGGGAG 
CTCAGCCAGA TGCAGCAGGA GTACGCGGAG CTCAAGAAGC AGATGGTAAA CCAGAAAGAT 
CTGGAAAGAA GACAAGTAGA AATCAGTGAA GCGATGAGGA CACTTAAGTC TGAAGTGAAA 
GACGAGATCA GAGCCAGCCT GAAGAACCTC AACCAGTTTC TCCCGGAGCT GCCAGCAGAC 
CTGGAGGCCA TTCTGGAAAG GAGCGAACAC CTAGACGGAG GGCTGGAGAG CTTGAAGGAG 
AACTTTCCAT TCGCCCTGAG TGAGAAGCCA TCACCTTTGG AAGAGAAACT ACATTTTTCT 
CGCGTTCACA TAATGGATGA GCACTGGCGT GGAGAAGCGC TCCGGGAGAA GCTGCGGCAC 
CGTGAGGACC GACTCAAGGC CCAGCTCCGA CACTGTATGT CCAAGCAGGC AGAGGTGTTA 
AGCAAAGGGA AGCGGCAGAC CGAGGGCACG TTACACAGCC TGAGGAGGCA GGTCGACGCC 
TTAGGGGAGC TGGTCACCAG CACGTCTGTG GAGTCCACGT CGTCAGCCAC TCTGTCTCAG 
CTCGAATCTT CCCTTGCAGA GGACTCCCAT CTTGGACCAA GTCAGAGCTC CTTCCATCAA 
GTTCCACAAG GTCCATCACA ATCAGTAGAC AGTTACCGAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025138136.1
CDS	356..7420
Translation	MKKGPQQKISKTKMPPSSHSPSPSSLTSNMRSRSLSPLNGSETLPFHSGRQWYGQVEIIGENSMLLDYHDQKEADSHAGVRYITEALVKKLTKQENLTLVKSLNLSLSKDGGKKFKYIENLEKCTKLEILNLSHNLIGKIEKVDKLLKLRELNLSYNKICKIEGIENMHNLQKLNLAGNEIEHIPAWLGKKLKCLRVLNLKANKISSLQDVSKLKPLQDLTSLILAENPVVTLPHYLQFTIFHLRSLESLEGQPVTTQDRQEAFERFSLEEVERLERDLEKKMMETEELKSKQAQFLEEIKNQDKLNRSLKEEAMLQKQSCEELESNLNTKNELPLSRFLADPCLPILYSSLKLRQKTMELTRACQKQYELEQELAFYKIDAKFEPLNYYPSEYVDVDKAPDESPYIGKSRYKRNMFATESYIVNNAQPVEIKRMEPEEGEQLRNEHVSLKAHMPLDTELEDKENKISTAQARLSALHDEIEKAEQQILRATEEFKQLEEAVQLKKISEAEKDFLLKQLSGRIQLLNKLRQEALDLEMQMEKQRGEIAEKQEEIKDLQRVIDSLDSKDPKHCHMKAQKRGKEQQFDIMNKQYKQLESRLDEILSRIAKETEEIKDLEQQLTEGQIAANEALKKDLEGVISGLQEYLGTVKGQAAQAQSECRRLQDEKEALLQRLAEVEQERDQLEAVALDAEAMRKELAELESALQEQHEVNASLQQTHGDLSAYEAELEAQLRMRDAEASQLKEELGELTRLSQLEQSTLQTELEKEKQALKNALGKAQLSEEKEQENSELRTQLKQLQDDNNLLKQQLKDFQNHLNQVADGLIRPEEVAARVDEVRRKLKSGAGEMSRIHSPSDVLGKSLADLQIQFSEMLTRSQWEKEEAQGRERKLQEEMALHQEKLVDGQEAFRQACERALEARINFDKRQHEARIQQLENEIHYLQENLKSMEEIQGLTDLQLQEADEEKERILAQLQELEKKKKLEDAKSQAQVLGLDKELKKLKKAVATSDKIATAELTIAKDQLKSLHGTVMKINQERAEDLQEAERFSRKAAQAARDLTRAEAEIELLQNLLREREEQMEKVEAGTRGASSQALEIERWNETMERQRSEIARLRDSLDRTGADNRGGIENILEEIAELRREVSYQNDYISSMADPFKRRGYWYFMPPPSSSKVSSHSSQATKDSGVGMKYAASTPIRKPQQNGREDGAPPAASGYWVYSPIRSGLHKSFSNKDADSGGESREESELDDHDDRPFVPPPGYMMYTVFPDGSPVPQGMALYAPPPPLPNNSRPLSPGTVVYGPPPAGAPIVYGPPPPSFSVPLIPVGVLHCNVPEHHALENEVSRLEDIIQHLKSKKQEQRMRVCKRQSEKEIEELHHNIDELLQEKKDLEHEVEELHRTIQKHQQQKDFIDGNVESLMNELKIEKSLKHHEDIVDEIECIEKTLLKRRAELREADRLLAEAESELSRTTEKTKNAVEKFTDAKRNLLQTEKDAEELERRAQETAVSLVKADQQLRLLQADTKDLEQHKTEQEEILKEINKVVAAKDSDFQCLNTKKEKLTEELQKLQKDIETAARTEDHHLQVLRESEALLQAKRAELDKLRSQVTSQQQEMAVLDRQLGHKKEELHLLQGSMVQARADLQEALRLGETEVTEKCNHIRELKGLLEELSFQKGELNVQISEKKNQLSLIKQEIEKEEENLQVVLGQMSKHKTELKNILDMLQLENNELQALKLQHEQKMSELEKAQVEVLEEKLELENLQQTALRQKGEIEWQKQLLERDKREIERITAETRALQLCVESLCKQKEDLEEKCDSWEKKLAQTKRVLAATEENSKAKQSDLEKLELDIRKLEQELDQLNRNKLSLHRDIAAMQQQVQEKREAVNSLQEELADVQAHLNLAKQDLLHTTKRQDVLLGEQTRLQEDLQERRKKLEVCQKEEATKRQHLQALQTEMEANEAELARQETTFQRLQKERESEEKKLEASRATLQEQQQQLEKEIADQKNVLDQVLSQVLGAKERARTLQQEERWGETLEKTLSQTQQQLSEREQQLLEKSAELLALQKEADSMRADFSLLRNQFLTERKKAEKQVASLREALKTQRSQLEKTLLEQKQENSSMQKEMATIEQVAQDNHERARRLMRELSQMQQEYAELKKQMVNQKDLERRQVEISEAMRTLKSEVKDEIRASLKNLNQFLPELPADLEAILERSEHLDGGLESLKENFPFALSEKPSPLEEKLHFSRVHIMDEHWRGEALREKLRHREDRLKAQLRHCMSKQAEVLSKGKRQTEGTLHSLRRQVDALGELVTSTSVESTSSATLSQLESSLAEDSHLGPSQSSFHQVPQGPSQSVDSYRH

Target ORF information:

RefSeq Version	XM_025282351.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centriolin (CNTRL), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025282351.1

ORF Insert Sequence:

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCCCTTGTC TCGGTTTCTT 
GCGGACCCTT GTCTGCCGAT TCTTTATTCT TCCTTAAAGC TAAGACAGAA GACCATGGAA 
CTAACGCGAG CATGTCAAAA ACAGTATGAA CTGGAACAGG AGTTGGCTTT TTACAAAATC 
GATGCTAAAT TTGAGCCACT GAATTATTAT CCCTCAGAGT ATGTTGACGT TGATAAAGCC 
CCAGATGAAA GCCCTTACAT TGGCAAGTCC AGATACAAGA GAAATATGTT CGCCACAGAG 
AGTTACATTG TGAATAATGC CCAGCCAGTT GAGATCAAGA GGATGGAGCC AGAGGAAGGG 
GAGCAACTTA GAAACGAGCA TGTGAGCTTG AAAGCCCATA TGCCACTGGA CACGGAGCTG 
GAAGACAAAG AGAACAAAAT AAGTACAGCA CAAGCTCGAC TATCAGCACT GCATGATGAA 
ATAGAAAAGG CAGAACAACA AATTTTAAGA GCCACTGAAG AATTTAAACA ACTGGAAGAA 
GCTGTACAAC TTAAAAAGAT TTCAGAAGCA GAGAAAGACT TTCTTCTCAA GCAGCTGAGT 
GGCAGGATAC AGCTTCTAAA TAAATTACGC CAGGAAGCTC TTGATCTAGA AATGCAGATG 
GAAAAGCAAA GAGGAGAAAT TGCTGAAAAG CAGGAGGAAA TCAAGGACCT CCAAAGAGTT 
ATTGATAGTC TGGATTCCAA AGACCCAAAA CATTGCCATA TGAAGGCTCA GAAAAGGGGT 
AAAGAACAAC AATTTGACAT TATGAACAAG CAGTACAAAC AACTTGAGAG CCGTTTGGAC 
GAGATACTTT CTAGAATTGC TAAAGAAACT GAAGAGATTA AGGACCTTGA ACAACAGCTT 
ACTGAAGGCC AGATAGCAGC AAATGAAGCC CTGAAGAAGG ACTTAGAAGG CGTCATCAGT 
GGCCTGCAAG AATACCTGGG CACTGTGAAG GGCCAGGCCG CTCAGGCCCA GAGCGAGTGC 
AGAAGGCTGC AGGATGAGAA AGAGGCGCTG CTGCAGAGGT TGGCCGAGGT CGAGCAGGAA 
AGGGACCAGC TGGAAGCAGT CGCCTTAGAT GCAGAAGCTA TGAGGAAGGA GCTTGCAGAG 
CTAGAAAGCG CCCTCCAAGA GCAGCATGAG GTGAACGCGT CCCTGCAGCA GACCCACGGA 
GACCTCAGTG CCTACGAGGC TGAGCTAGAG GCACAGCTGA GGATGAGGGA TGCCGAAGCC 
AGCCAGCTCA AAGAAGAGCT GGGAGAACTA ACAAGGCTCA GCCAGCTAGA ACAGTCAACT 
CTACAAACAG AACTTGAGAA AGAAAAACAA GCCCTCAAGA ACGCCCTGGG AAAAGCCCAG 
CTCTCGGAAG AAAAAGAACA AGAAAACAGT GAGCTCCGCA CCCAGCTTAA ACAGCTACAG 
GATGACAATA ACCTGTTAAA ACAGCAACTT AAAGATTTCC AGAATCACCT TAATCAGGTA 
GCTGATGGTC TGATTCGTCC AGAAGAAGTG GCAGCTCGTG TAGATGAAGT AAGGAGAAAA 
TTGAAATCAG GCGCTGGAGA GATGAGCAGA ATCCACAGTC CTTCAGATGT CTTAGGGAAA 
AGTCTTGCTG ACTTGCAGAT ACAATTCAGT GAAATGCTGA CACGTTCTCA GTGGGAGAAA 
GAGGAAGCCC AAGGGAGGGA AAGAAAACTC CAAGAAGAAA TGGCTCTGCA TCAGGAGAAG 
CTGGTGGATG GACAGGAAGC GTTCAGGCAG GCCTGTGAGA GAGCGCTAGA AGCAAGGATT 
AATTTTGACA AGAGGCAACA TGAAGCAAGA ATTCAGCAGT TAGAGAATGA GATTCACTAT 
TTACAAGAAA ATCTAAAAAG TATGGAGGAA ATCCAAGGGC TTACAGATCT GCAGCTGCAG 
GAAGCTGATG AAGAGAAGGA GAGAATTCTA GCCCAACTCC AGGAGTTAGA GAAGAAGAAG 
AAACTTGAAG ATGCCAAATC TCAGGCACAG GTTCTAGGTT TAGATAAAGA ATTGAAGAAA 
CTAAAGAAGG CTGTGGCCAC CTCTGATAAG ATAGCCACAG CTGAGCTCAC CATCGCCAAA 
GACCAGCTCA AGTCCCTTCA TGGAACTGTT ATGAAAATTA ACCAGGAGCG TGCAGAGGAC 
CTGCAGGAAG CAGAGCGGTT CAGCAGAAAG GCAGCACAGG CCGCGAGGGA TCTGACCCGA 
GCGGAAGCGG AGATCGAACT CCTGCAGAAC CTCCTCAGGG AGAGAGAGGA GCAGATGGAG 
AAGGTAGAGG CAGGCACCCG AGGAGCAAGC TCACAGGCGC TGGAAATCGA GAGGTGGAAT 
GAGACCATGG AGAGACAGAG GAGCGAGATC GCAAGGCTAC GGGACTCACT AGACCGCACC 
GGGGCGGATA ACAGAGGAGG CATTGAAAAT ATTTTGGAAG AAATAGCTGA ACTCCGACGT 
GAAGTTTCTT ACCAGAATGA TTACATCAGC AGCATGGCAG ATCCTTTCAA AAGACGAGGC 
TATTGGTACT TTATGCCACC ACCATCATCA TCAAAAGTTT CCAGCCACAG CTCCCAGGCC 
ACAAAGGACT CTGGTGTCGG GATGAAGTAC GCAGCCTCGA CTCCTATTAG AAAACCACAG 
CAGAACGGGA GGGAAGACGG TGCCCCTCCA GCTGCCTCCG GATACTGGGT GTATTCCCCC 
ATCAGGAGCG GCTTACATAA ATCGTTTTCA AATAAAGATG CAGACAGTGG AGGAGAGAGC 
CGGGAGGAGA GCGAGCTGGA TGACCACGAT GACCGCCCGT TCGTCCCTCC TCCTGGATAC 
ATGATGTACA CCGTGTTCCC CGACGGCTCT CCTGTACCAC AGGGCATGGC CCTCTACGCA 
CCGCCGCCTC CCTTGCCCAA CAATAGCCGA CCTCTCAGCC CTGGCACTGT TGTGTATGGC 
CCGCCCCCTG CTGGTGCCCC CATTGTCTAC GGGCCCCCAC CGCCCAGCTT CTCCGTCCCC 
CTCATCCCAG TGGGCGTGCT GCACTGCAAC GTCCCGGAGC ACCATGCCTT AGAGAATGAG 
GTTTCTAGAT TGGAAGACAT AATACAGCAT TTAAAATCAA AGAAGCAGGA ACAGAGGATG 
AGAGTATGCA AGCGGCAGTC TGAGAAAGAA ATAGAAGAAC TGCATCATAA TATTGATGAA 
CTTTTGCAAG AGAAGAAAGA CTTAGAGCAC GAAGTAGAAG AATTACATAG AACCATCCAG 
AAACATCAAC AGCAAAAAGA CTTCATTGAT GGAAATGTTG AGAGTCTTAT GAATGAACTA 
AAAATAGAAA AATCACTCAA GCACCATGAA GATATTGTAG ATGAAATTGA GTGCATCGAG 
AAGACTCTCC TGAAACGTCG AGCAGAGCTC AGGGAGGCTG ACCGACTGCT GGCGGAAGCC 
GAGAGTGAAC TTTCACGCAC AACAGAGAAA ACAAAAAATG CTGTTGAAAA GTTCACTGAT 
GCCAAGAGGA ATTTGTTGCA AACTGAGAAA GATGCTGAGG AGTTAGAAAG GAGAGCTCAG 
GAAACAGCTG TTAGCCTTGT CAAGGCTGAT CAACAGCTCA GATTGTTACA GGCGGACACA 
AAGGATTTGG AGCAGCACAA AACTGAGCAA GAAGAGATCT TGAAAGAGAT AAACAAAGTT 
GTTGCAGCAA AAGACTCAGA CTTCCAGTGT TTAAACACGA AGAAGGAGAA GCTAACAGAA 
GAGCTGCAGA AGCTGCAGAA AGACATCGAG ACGGCAGCGC GCACGGAGGA TCACCACCTG 
CAGGTCCTGA GAGAGTCGGA GGCCCTCCTG CAGGCCAAGA GAGCGGAGCT GGACAAGCTG 
AGGAGCCAGG TAACGAGTCA GCAGCAGGAG ATGGCTGTCT TGGACAGACA GCTGGGGCAT 
AAGAAGGAGG AGCTGCATCT ACTCCAGGGA AGCATGGTCC AGGCCAGAGC TGACCTCCAG 
GAAGCGCTGA GGCTGGGAGA AACTGAAGTA ACTGAGAAGT GCAACCACAT CAGGGAACTA 
AAAGGTCTTC TGGAAGAACT GAGTTTTCAG AAAGGAGAAC TGAATGTCCA GATTAGTGAG 
AAAAAAAATC AACTTTCACT TATAAAGCAA GAAATTGAAA AAGAGGAAGA AAATCTTCAG 
GTAGTTTTAG GGCAGATGTC TAAACATAAA ACTGAACTTA AGAATATTCT GGACATGTTG 
CAACTTGAAA ACAATGAGCT GCAAGCTTTG AAGCTACAGC ATGAGCAAAA GATGTCTGAG 
TTAGAGAAGG CTCAGGTTGA AGTGCTGGAG GAGAAACTGG AGTTGGAGAA TTTGCAGCAG 
ACAGCCCTGC GACAGAAAGG GGAGATCGAG TGGCAGAAGC AGCTCTTAGA GAGGGACAAG 
CGAGAGATTG AGCGGATAAC TGCTGAGACC CGAGCATTGC AGTTGTGTGT TGAGTCTTTG 
TGCAAACAAA AGGAAGATCT TGAGGAGAAA TGTGACAGCT GGGAGAAGAA GCTGGCACAG 
ACCAAACGGG TTTTAGCAGC CACAGAAGAG AATAGCAAAG CAAAGCAATC AGACTTAGAA 
AAGTTGGAAT TGGATATCAG AAAACTGGAA CAGGAACTAG ACCAGCTAAA CAGAAACAAA 
CTCTCACTGC ACAGAGACAT CGCGGCCATG CAGCAGCAGG TCCAAGAAAA ACGAGAAGCA 
GTAAACTCAT TACAGGAGGA ACTAGCTGAT GTCCAAGCCC ATTTGAACCT AGCGAAACAG 
GACCTGCTCC ACACCACCAA GCGCCAGGAT GTCCTGCTTG GCGAGCAGAC CAGGCTGCAG 
GAGGACCTCC AGGAACGCAG AAAGAAGTTG GAAGTCTGCC AAAAAGAAGA GGCAACAAAG 
CGGCAGCACC TGCAAGCGCT TCAGACGGAG ATGGAGGCGA ACGAGGCCGA GCTGGCCCGG 
CAGGAGACGA CATTTCAGAG ACTCCAGAAA GAGAGAGAAA GTGAAGAAAA AAAGTTAGAA 
GCCAGTCGAG CGACTCTGCA GGAGCAGCAG CAGCAGCTGG AAAAGGAAAT CGCAGACCAG 
AAGAACGTGC TGGACCAGGT GCTCAGCCAG GTGCTGGGGG CCAAGGAGCG CGCCAGGACT 
CTGCAGCAGG AGGAGAGGTG GGGTGAGACC CTGGAGAAGA CGCTCTCCCA GACCCAGCAA 
CAGCTCTCAG AACGGGAGCA GCAGTTGTTG GAAAAGTCAG CTGAGCTGCT GGCTCTTCAG 
AAAGAGGCAG ACTCGATGAG GGCAGACTTC AGCCTCTTGC GAAACCAGTT CTTGACGGAG 
AGGAAGAAAG CCGAGAAGCA GGTAGCCAGC CTCAGGGAAG CGCTCAAGAC CCAGCGCAGC 
CAGCTGGAGA AGACCCTTCT CGAGCAGAAG CAGGAGAACA GCTCCATGCA GAAGGAGATG 
GCGACTATCG AGCAGGTGGC GCAGGACAAC CACGAGCGGG CCCGGCGCCT GATGCGGGAG 
CTCAGCCAGA TGCAGCAGGA GTACGCGGAG CTCAAGAAGC AGATGGTAAA CCAGAAAGAT 
CTGGAAAGAA GACAAGTAGA AATCAGTGAA GCGATGAGGA CACTTAAGTC TGAAGTGAAA 
GACGAGATCA GAGCCAGCCT GAAGAACCTC AACCAGTTTC TCCCGGAGCT GCCAGCAGAC 
CTGGAGGCCA TTCTGGAAAG GAGCGAACAC CTAGACGGAG GGCTGGAGAG CTTGAAGGAG 
AACTTTCCAT TCGCCCTGAG TGAGAAGCCA TCACCTTTGG AAGAGAAACT ACATTTTTCT 
CGCGTTCACA TAATGGATGA GCACTGGCGT GGAGAAGCGC TCCGGGAGAA GCTGCGGCAC 
CGTGAGGACC GACTCAAGGC CCAGCTCCGA CACTGTATGT CCAAGCAGGC AGAGGTGTTA 
AGCAAAGGGA AGCGGCAGAC CGAGGGCACG TTACACAGCC TGAGGAGGCA GGTCGACGCC 
TTAGGGGAGC TGGTCACCAG CACGTCTGTG GAGTCCACGT CGTCAGCCAC TCTGTCTCAG 
CTCGAATCTT CCCTTGCAGA GGACTCCCAT CTTGGACCAA GTCAGAGCTC CTTCCATCAA 
GTTCCACAAG GTCCATCACA ATCAGTAGAC AGTTACCGAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTRL ( XM_025282354.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025138139.1 Bubalus bubalis centriolin (CNTRL), transcript variant X12, mRNA.

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CloneID	OBu63441
Clone ID Related Accession (Same CDS sequence)	XM_025282354.1
Accession Version	XM_025282354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	centriolin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCTAAGACA GAAGACCATG 
GAACTAACGC GAGCATGTCA AAAACAGTAT GAACTGGAAC AGGAGTTGGC TTTTTACAAA 
ATCGATGCTA AATTTGAGCC ACTGAATTAT TATCCCTCAG AGTATGTTGA CGTTGATAAA 
GCCCCAGATG AAAGCCCTTA CATTGGCAAG TCCAGATACA AGAGAAATAT GTTCGCCACA 
GAGAGTTACA TTGTGAATAA TGCCCAGCCA GTTGAGATCA AGAGGATGGA GCCAGAGGAA 
GGGGAGCAAC TTAGAAACGA GCATGTGAGC TTGAAAGCCC ATATGCCACT GGACACGGAG 
CTGGAAGACA AAGAGAACAA AATAAGTACA GCACAAGCTC GACTATCAGC ACTGCATGAT 
GAAATAGAAA AGGCAGAACA ACAAATTTTA AGAGCCACTG AAGAATTTAA ACAACTGGAA 
GAAGCTGTAC AACTTAAAAA GATTTCAGAA GCAGAGAAAG ACTTTCTTCT CAAGCAGCTG 
AGTGGCAGGA TACAGCTTCT AAATAAATTA CGCCAGGAAG CTCTTGATCT AGAAATGCAG 
ATGGAAAAGC AAAGAGGAGA AATTGCTGAA AAGCAGGAGG AAATCAAGGA CCTCCAAAGA 
GTTATTGATA GTCTGGATTC CAAAGACCCA AAACATTGCC ATATGAAGGC TCAGAAAAGG 
GGTAAAGAAC AACAATTTGA CATTATGAAC AAGCAGTACA AACAACTTGA GAGCCGTTTG 
GACGAGATAC TTTCTAGAAT TGCTAAAGAA ACTGAAGAGA TTAAGGACCT TGAACAACAG 
CTTACTGAAG GCCAGATAGC AGCAAATGAA GCCCTGAAGA AGGACTTAGA AGGCGTCATC 
AGTGGCCTGC AAGAATACCT GGGCACTGTG AAGGGCCAGG CCGCTCAGGC CCAGAGCGAG 
TGCAGAAGGC TGCAGGATGA GAAAGAGGCG CTGCTGCAGA GGTTGGCCGA GGTCGAGCAG 
GAAAGGGACC AGCTGGAAGC AGTCGCCTTA GATGCAGAAG CTATGAGGAA GGAGCTTGCA 
GAGCTAGAAA GCGCCCTCCA AGAGCAGCAT GAGGTGAACG CGTCCCTGCA GCAGACCCAC 
GGAGACCTCA GTGCCTACGA GGCTGAGCTA GAGGCACAGC TGAGGATGAG GGATGCCGAA 
GCCAGCCAGC TCAAAGAAGA GCTGGGAGAA CTAACAAGGC TCAGCCAGCT AGAACAGTCA 
ACTCTACAAA CAGAACTTGA GAAAGAAAAA CAAGCCCTCA AGAACGCCCT GGGAAAAGCC 
CAGCTCTCGG AAGAAAAAGA ACAAGAAAAC AGTGAGCTCC GCACCCAGCT TAAACAGCTA 
CAGGATGACA ATAACCTGTT AAAACAGCAA CTTAAAGATT TCCAGAATCA CCTTAATCAG 
GTAGCTGATG GTCTGATTCG TCCAGAAGAA GTGGCAGCTC GTGTAGATGA AGTAAGGAGA 
AAATTGAAAT CAGGCGCTGG AGAGATGAGA ATCCACAGTC CTTCAGATGT CTTAGGGAAA 
AGTCTTGCTG ACTTGCAGAT ACAATTCAGT GAAATGCTGA CACGTTCTCA GTGGGAGAAA 
GAGGAAGCCC AAGGGAGGGA AAGAAAACTC CAAGAAGAAA TGGCTCTGCA TCAGGAGAAG 
CTGGTGGATG GACAGGAAGC GTTCAGGCAG GCCTGTGAGA GAGCGCTAGA AGCAAGGATT 
AATTTTGACA AGAGGCAACA TGAAGCAAGA ATTCAGCAGT TAGAGAATGA GATTCACTAT 
TTACAAGAAA ATCTAAAAAG TATGGAGGAA ATCCAAGGGC TTACAGATCT GCAGCTGCAG 
GAAGCTGATG AAGAGAAGGA GAGAATTCTA GCCCAACTCC AGGAGTTAGA GAAGAAGAAG 
AAACTTGAAG ATGCCAAATC TCAGGCACAG GTTCTAGGTT TAGATAAAGA ATTGAAGAAA 
CTAAAGAAGG CTGTGGCCAC CTCTGATAAG ATAGCCACAG CTGAGCTCAC CATCGCCAAA 
GACCAGCTCA AGTCCCTTCA TGGAACTGTT ATGAAAATTA ACCAGGAGCG TGCAGAGGAC 
CTGCAGGAAG CAGAGCGGTT CAGCAGAAAG GCAGCACAGG CCGCGAGGGA TCTGACCCGA 
GCGGAAGCGG AGATCGAACT CCTGCAGAAC CTCCTCAGGG AGAGAGAGGA GCAGATGGAG 
AAGGTAGAGG CAGGCACCCG AGGAGCAAGC TCACAGGCGC TGGAAATCGA GAGGTGGAAT 
GAGACCATGG AGAGACAGAG GAGCGAGATC GCAAGGCTAC GGGACTCACT AGACCGCACC 
GGGGCGGATA ACAGAGGAGG CATTGAAAAT ATTTTGGAAG AAATAGCTGA ACTCCGACGT 
GAAGTTTCTT ACCAGAATGA TTACATCAGC AGCATGGCAG ATCCTTTCAA AAGACGAGGC 
TATTGGTACT TTATGCCACC ACCATCATCA TCAAAAGTTT CCAGCCACAG CTCCCAGGCC 
ACAAAGGACT CTGGTGTCGG GATGAAGTAC GCAGCCTCGA CTCCTATTAG AAAACCACAG 
CAGAACGGGA GGGAAGACGG TGCCCCTCCA GCTGCCTCCG GATACTGGGT GTATTCCCCC 
ATCAGGAGCG GCTTACATAA ATCGTTTTCA AATAAAGATG CAGACAGTGG AGGAGAGAGC 
CGGGAGGAGA GCGAGCTGGA TGACCACGAT GACCGCCCGT TCGTCCCTCC TCCTGGATAC 
ATGATGTACA CCGTGTTCCC CGACGGCTCT CCTGTACCAC AGGGCATGGC CCTCTACGCA 
CCGCCGCCTC CCTTGCCCAA CAATAGCCGA CCTCTCAGCC CTGGCACTGT TGTGTATGGC 
CCGCCCCCTG CTGGTGCCCC CATTGTCTAC GGGCCCCCAC CGCCCAGCTT CTCCGTCCCC 
CTCATCCCAG TGGGCGTGCT GCACTGCAAC GTCCCGGAGC ACCATGCCTT AGAGAATGAG 
GTTTCTAGAT TGGAAGACAT AATACAGCAT TTAAAATCAA AGAAGCAGGA ACAGAGGATG 
AGAGTATGCA AGCGGCAGTC TGAGAAAGAA ATAGAAGAAC TGCATCATAA TATTGATGAA 
CTTTTGCAAG AGAAGAAAGA CTTAGAGCAC GAAGTAGAAG AATTACATAG AACCATCCAG 
AAACATCAAC AGCAAAAAGA CTTCATTGAT GGAAATGTTG AGAGTCTTAT GAATGAACTA 
AAAATAGAAA AATCACTCAA GCACCATGAA GATATTGTAG ATGAAATTGA GTGCATCGAG 
AAGACTCTCC TGAAACGTCG AGCAGAGCTC AGGGAGGCTG ACCGACTGCT GGCGGAAGCC 
GAGAGTGAAC TTTCACGCAC AACAGAGAAA ACAAAAAATG CTGTTGAAAA GTTCACTGAT 
GCCAAGAGGA ATTTGTTGCA AACTGAGAAA GATGCTGAGG AGTTAGAAAG GAGAGCTCAG 
GAAACAGCTG TTAGCCTTGT CAAGGCTGAT CAACAGCTCA GATTGTTACA GGCGGACACA 
AAGGATTTGG AGCAGCACAA AACTGAGCAA GAAGAGATCT TGAAAGAGAT AAACAAAGTT 
GTTGCAGCAA AAGACTCAGA CTTCCAGTGT TTAAACACGA AGAAGGAGAA GCTAACAGAA 
GAGCTGCAGA AGCTGCAGAA AGACATCGAG ACGGCAGCGC GCACGGAGGA TCACCACCTG 
CAGGTCCTGA GAGAGTCGGA GGCCCTCCTG CAGGCCAAGA GAGCGGAGCT GGACAAGCTG 
AGGAGCCAGG TAACGAGTCA GCAGCAGGAG ATGGCTGTCT TGGACAGACA GCTGGGGCAT 
AAGAAGGAGG AGCTGCATCT ACTCCAGGGA AGCATGGTCC AGGCCAGAGC TGACCTCCAG 
GAAGCGCTGA GGCTGGGAGA AACTGAAGTA ACTGAGAAGT GCAACCACAT CAGGGAACTA 
AAAGGTCTTC TGGAAGAACT GAGTTTTCAG AAAGGAGAAC TGAATGTCCA GATTAGTGAG 
AAAAAAAATC AACTTTCACT TATAAAGCAA GAAATTGAAA AAGAGGAAGA AAATCTTCAG 
GTAGTTTTAG GGCAGATGTC TAAACATAAA ACTGAACTTA AGAATATTCT GGACATGTTG 
CAACTTGAAA ACAATGAGCT GCAAGCTTTG AAGCTACAGC ATGAGCAAAA GATGTCTGAG 
TTAGAGAAGG CTCAGGTTGA AGTGCTGGAG GAGAAACTGG AGTTGGAGAA TTTGCAGCAG 
ACAGCCCTGC GACAGAAAGG GGAGATCGAG TGGCAGAAGC AGCTCTTAGA GAGGGACAAG 
CGAGAGATTG AGCGGATAAC TGCTGAGACC CGAGCATTGC AGTTGTGTGT TGAGTCTTTG 
TGCAAACAAA AGGAAGATCT TGAGGAGAAA TGTGACAGCT GGGAGAAGAA GCTGGCACAG 
ACCAAACGGG TTTTAGCAGC CACAGAAGAG AATAGCAAAG CAAAGCAATC AGACTTAGAA 
AAGTTGGAAT TGGATATCAG AAAACTGGAA CAGGAACTAG ACCAGCTAAA CAGAAACAAA 
CTCTCACTGC ACAGAGACAT CGCGGCCATG CAGCAGCAGG TCCAAGAAAA ACGAGAAGCA 
GTAAACTCAT TACAGGAGGA ACTAGCTGAT GTCCAAGCCC ATTTGAACCT AGCGAAACAG 
GACCTGCTCC ACACCACCAA GCGCCAGGAT GTCCTGCTTG GCGAGCAGAC CAGGCTGCAG 
GAGGACCTCC AGGAACGCAG AAAGAAGTTG GAAGTCTGCC AAAAAGAAGA GGCAACAAAG 
CGGCAGCACC TGCAAGCGCT TCAGACGGAG ATGGAGGCGA ACGAGGCCGA GCTGGCCCGG 
CAGGAGACGA CATTTCAGAG ACTCCAGAAA GAGAGAGAAA GTGAAGAAAA AAAGTTAGAA 
GCCAGTCGAG CGACTCTGCA GGAGCAGCAG CAGCAGCTGG AAAAGGAAAT CGCAGACCAG 
AAGAACGTGC TGGACCAGGT GCTCAGCCAG GTGCTGGGGG CCAAGGAGCG CGCCAGGACT 
CTGCAGCAGG AGGAGAGGTG GGGTGAGACC CTGGAGAAGA CGCTCTCCCA GACCCAGCAA 
CAGCTCTCAG AACGGGAGCA GCAGTTGTTG GAAAAGTCAG CTGAGCTGCT GGCTCTTCAG 
AAAGAGGCAG ACTCGATGAG GGCAGACTTC AGCCTCTTGC GAAACCAGTT CTTGACGGAG 
AGGAAGAAAG CCGAGAAGCA GGTAGCCAGC CTCAGGGAAG CGCTCAAGAC CCAGCGCAGC 
CAGCTGGAGA AGACCCTTCT CGAGCAGAAG CAGGAGAACA GCTCCATGCA GAAGGAGATG 
GCGACTATCG AGCAGGTGGC GCAGGACAAC CACGAGCGGG CCCGGCGCCT GATGCGGGAG 
CTCAGCCAGA TGCAGCAGGA GTACGCGGAG CTCAAGAAGC AGATGGTAAA CCAGAAAGAT 
CTGGAAAGAA GACAAGTAGA AATCAGTGAA GCGATGAGGA CACTTAAGTC TGAAGTGAAA 
GACGAGATCA GAGCCAGCCT GAAGAACCTC AACCAGTTTC TCCCGGAGCT GCCAGCAGAC 
CTGGAGGCCA TTCTGGAAAG GAGCGAACAC CTAGACGGAG GGCTGGAGAG CTTGAAGGAG 
AACTTTCCAT TCGCCCTGAG TGAGAAGCCA TCACCTTTGG AAGAGAAACT ACATTTTTCT 
CGCGTTCACA TAATGGATGA GCACTGGCGT GGAGAAGCGC TCCGGGAGAA GCTGCGGCAC 
CGTGAGGACC GACTCAAGGC CCAGCTCCGA CACTGTATGT CCAAGCAGGC AGAGGTGTTA 
AGCAAAGGGA AGCGGCAGAC CGAGGGCACG TTACACAGCC TGAGGAGGCA GGTCGACGCC 
TTAGGGGAGC TGGTCACCAG CACGTCTGTG GAGTCCACGT CGTCAGCCAC TCTGTCTCAG 
CTCGAATCTT CCCTTGCAGA GGACTCCCAT CTTGGACCAA GTCAGAGCTC CTTCCATCAA 
GTTCCACAAG GTCCATCACA ATCAGTAGAC AGTTACCGAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025138139.1
CDS	364..7368
Translation	MKKGPQQKISKTKMPPSSHSPSPSSLTSNMRSRSLSPLNGSETLPFHSGRQWYGQVEIIGENSMLLDYHDQKEADSHAGVRYITEALVKKLTKQENLTLVKSLNLSLSKDGGKKFKYIENLEKCTKLEILNLSHNLIGKIEKVDKLLKLRELNLSYNKICKIEGIENMHNLQKLNLAGNEIEHIPAWLGKKLKCLRVLNLKANKISSLQDVSKLKPLQDLTSLILAENPVVTLPHYLQFTIFHLRSLESLEGQPVTTQDRQEAFERFSLEEVERLERDLEKKMMETEELKSKQAQFLEEIKNQDKLNRSLKEEAMLQKQSCEELESNLNTKNELLRQKTMELTRACQKQYELEQELAFYKIDAKFEPLNYYPSEYVDVDKAPDESPYIGKSRYKRNMFATESYIVNNAQPVEIKRMEPEEGEQLRNEHVSLKAHMPLDTELEDKENKISTAQARLSALHDEIEKAEQQILRATEEFKQLEEAVQLKKISEAEKDFLLKQLSGRIQLLNKLRQEALDLEMQMEKQRGEIAEKQEEIKDLQRVIDSLDSKDPKHCHMKAQKRGKEQQFDIMNKQYKQLESRLDEILSRIAKETEEIKDLEQQLTEGQIAANEALKKDLEGVISGLQEYLGTVKGQAAQAQSECRRLQDEKEALLQRLAEVEQERDQLEAVALDAEAMRKELAELESALQEQHEVNASLQQTHGDLSAYEAELEAQLRMRDAEASQLKEELGELTRLSQLEQSTLQTELEKEKQALKNALGKAQLSEEKEQENSELRTQLKQLQDDNNLLKQQLKDFQNHLNQVADGLIRPEEVAARVDEVRRKLKSGAGEMRIHSPSDVLGKSLADLQIQFSEMLTRSQWEKEEAQGRERKLQEEMALHQEKLVDGQEAFRQACERALEARINFDKRQHEARIQQLENEIHYLQENLKSMEEIQGLTDLQLQEADEEKERILAQLQELEKKKKLEDAKSQAQVLGLDKELKKLKKAVATSDKIATAELTIAKDQLKSLHGTVMKINQERAEDLQEAERFSRKAAQAARDLTRAEAEIELLQNLLREREEQMEKVEAGTRGASSQALEIERWNETMERQRSEIARLRDSLDRTGADNRGGIENILEEIAELRREVSYQNDYISSMADPFKRRGYWYFMPPPSSSKVSSHSSQATKDSGVGMKYAASTPIRKPQQNGREDGAPPAASGYWVYSPIRSGLHKSFSNKDADSGGESREESELDDHDDRPFVPPPGYMMYTVFPDGSPVPQGMALYAPPPPLPNNSRPLSPGTVVYGPPPAGAPIVYGPPPPSFSVPLIPVGVLHCNVPEHHALENEVSRLEDIIQHLKSKKQEQRMRVCKRQSEKEIEELHHNIDELLQEKKDLEHEVEELHRTIQKHQQQKDFIDGNVESLMNELKIEKSLKHHEDIVDEIECIEKTLLKRRAELREADRLLAEAESELSRTTEKTKNAVEKFTDAKRNLLQTEKDAEELERRAQETAVSLVKADQQLRLLQADTKDLEQHKTEQEEILKEINKVVAAKDSDFQCLNTKKEKLTEELQKLQKDIETAARTEDHHLQVLRESEALLQAKRAELDKLRSQVTSQQQEMAVLDRQLGHKKEELHLLQGSMVQARADLQEALRLGETEVTEKCNHIRELKGLLEELSFQKGELNVQISEKKNQLSLIKQEIEKEEENLQVVLGQMSKHKTELKNILDMLQLENNELQALKLQHEQKMSELEKAQVEVLEEKLELENLQQTALRQKGEIEWQKQLLERDKREIERITAETRALQLCVESLCKQKEDLEEKCDSWEKKLAQTKRVLAATEENSKAKQSDLEKLELDIRKLEQELDQLNRNKLSLHRDIAAMQQQVQEKREAVNSLQEELADVQAHLNLAKQDLLHTTKRQDVLLGEQTRLQEDLQERRKKLEVCQKEEATKRQHLQALQTEMEANEAELARQETTFQRLQKERESEEKKLEASRATLQEQQQQLEKEIADQKNVLDQVLSQVLGAKERARTLQQEERWGETLEKTLSQTQQQLSEREQQLLEKSAELLALQKEADSMRADFSLLRNQFLTERKKAEKQVASLREALKTQRSQLEKTLLEQKQENSSMQKEMATIEQVAQDNHERARRLMRELSQMQQEYAELKKQMVNQKDLERRQVEISEAMRTLKSEVKDEIRASLKNLNQFLPELPADLEAILERSEHLDGGLESLKENFPFALSEKPSPLEEKLHFSRVHIMDEHWRGEALREKLRHREDRLKAQLRHCMSKQAEVLSKGKRQTEGTLHSLRRQVDALGELVTSTSVESTSSATLSQLESSLAEDSHLGPSQSSFHQVPQGPSQSVDSYRH

Target ORF information:

RefSeq Version	XM_025282354.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis centriolin (CNTRL), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025282354.1

ORF Insert Sequence:

ATGAAGAAAG GTCCTCAGCA AAAAATTTCC AAAACAAAGA TGCCACCATC ATCTCACTCT 
CCTAGTCCAT CATCTCTTAC GTCCAATATG AGATCTAGGT CACTTTCACC TTTAAACGGA 
TCTGAGACTC TGCCTTTTCA TTCTGGAAGA CAGTGGTATG GGCAAGTCGA GATTATAGGT 
GAAAACTCTA TGCTTTTGGA CTACCATGAC CAGAAAGAAG CTGATTCACA TGCAGGAGTT 
CGATATATTA CAGAAGCCCT TGTTAAAAAA CTTACTAAAC AGGAAAATTT GACCTTGGTA 
AAATCTCTGA ACCTTTCACT TTCCAAAGAT GGTGGCAAGA AATTTAAGTA TATCGAAAAT 
TTGGAAAAAT GTACTAAGCT TGAAATACTG AATCTCAGCC ATAATCTCAT AGGGAAGATT 
GAGAAGGTGG ACAAGCTGTT AAAGTTACGT GAACTCAACC TATCATATAA CAAAATCTGC 
AAAATTGAAG GCATAGAAAA TATGCACAAT CTACAAAAGC TGAACCTTGC AGGAAATGAA 
ATTGAGCATA TTCCAGCGTG GTTAGGGAAG AAGTTAAAAT GTTTGCGAGT CCTCAATCTG 
AAAGCCAACA AGATATCATC GCTCCAAGAT GTAAGCAAAT TGAAACCACT TCAAGATTTG 
ACTTCTCTGA TCCTAGCTGA AAATCCAGTT GTGACCCTTC CCCATTACCT TCAGTTCACC 
ATTTTTCACC TCCGATCACT GGAAAGTTTG GAAGGTCAGC CAGTAACCAC TCAGGACAGG 
CAAGAAGCTT TTGAGAGATT CAGTTTAGAA GAGGTAGAAA GACTGGAAAG AGACCTGGAG 
AAGAAGATGA TGGAAACTGA AGAGCTTAAA AGCAAACAAG CACAGTTCCT TGAAGAAATT 
AAAAATCAAG ATAAGTTGAA CAGATCATTA AAAGAGGAGG CTATGCTACA AAAACAGAGC 
TGTGAGGAGC TCGAGAGTAA CCTTAACACG AAAAATGAAC TGCTAAGACA GAAGACCATG 
GAACTAACGC GAGCATGTCA AAAACAGTAT GAACTGGAAC AGGAGTTGGC TTTTTACAAA 
ATCGATGCTA AATTTGAGCC ACTGAATTAT TATCCCTCAG AGTATGTTGA CGTTGATAAA 
GCCCCAGATG AAAGCCCTTA CATTGGCAAG TCCAGATACA AGAGAAATAT GTTCGCCACA 
GAGAGTTACA TTGTGAATAA TGCCCAGCCA GTTGAGATCA AGAGGATGGA GCCAGAGGAA 
GGGGAGCAAC TTAGAAACGA GCATGTGAGC TTGAAAGCCC ATATGCCACT GGACACGGAG 
CTGGAAGACA AAGAGAACAA AATAAGTACA GCACAAGCTC GACTATCAGC ACTGCATGAT 
GAAATAGAAA AGGCAGAACA ACAAATTTTA AGAGCCACTG AAGAATTTAA ACAACTGGAA 
GAAGCTGTAC AACTTAAAAA GATTTCAGAA GCAGAGAAAG ACTTTCTTCT CAAGCAGCTG 
AGTGGCAGGA TACAGCTTCT AAATAAATTA CGCCAGGAAG CTCTTGATCT AGAAATGCAG 
ATGGAAAAGC AAAGAGGAGA AATTGCTGAA AAGCAGGAGG AAATCAAGGA CCTCCAAAGA 
GTTATTGATA GTCTGGATTC CAAAGACCCA AAACATTGCC ATATGAAGGC TCAGAAAAGG 
GGTAAAGAAC AACAATTTGA CATTATGAAC AAGCAGTACA AACAACTTGA GAGCCGTTTG 
GACGAGATAC TTTCTAGAAT TGCTAAAGAA ACTGAAGAGA TTAAGGACCT TGAACAACAG 
CTTACTGAAG GCCAGATAGC AGCAAATGAA GCCCTGAAGA AGGACTTAGA AGGCGTCATC 
AGTGGCCTGC AAGAATACCT GGGCACTGTG AAGGGCCAGG CCGCTCAGGC CCAGAGCGAG 
TGCAGAAGGC TGCAGGATGA GAAAGAGGCG CTGCTGCAGA GGTTGGCCGA GGTCGAGCAG 
GAAAGGGACC AGCTGGAAGC AGTCGCCTTA GATGCAGAAG CTATGAGGAA GGAGCTTGCA 
GAGCTAGAAA GCGCCCTCCA AGAGCAGCAT GAGGTGAACG CGTCCCTGCA GCAGACCCAC 
GGAGACCTCA GTGCCTACGA GGCTGAGCTA GAGGCACAGC TGAGGATGAG GGATGCCGAA 
GCCAGCCAGC TCAAAGAAGA GCTGGGAGAA CTAACAAGGC TCAGCCAGCT AGAACAGTCA 
ACTCTACAAA CAGAACTTGA GAAAGAAAAA CAAGCCCTCA AGAACGCCCT GGGAAAAGCC 
CAGCTCTCGG AAGAAAAAGA ACAAGAAAAC AGTGAGCTCC GCACCCAGCT TAAACAGCTA 
CAGGATGACA ATAACCTGTT AAAACAGCAA CTTAAAGATT TCCAGAATCA CCTTAATCAG 
GTAGCTGATG GTCTGATTCG TCCAGAAGAA GTGGCAGCTC GTGTAGATGA AGTAAGGAGA 
AAATTGAAAT CAGGCGCTGG AGAGATGAGA ATCCACAGTC CTTCAGATGT CTTAGGGAAA 
AGTCTTGCTG ACTTGCAGAT ACAATTCAGT GAAATGCTGA CACGTTCTCA GTGGGAGAAA 
GAGGAAGCCC AAGGGAGGGA AAGAAAACTC CAAGAAGAAA TGGCTCTGCA TCAGGAGAAG 
CTGGTGGATG GACAGGAAGC GTTCAGGCAG GCCTGTGAGA GAGCGCTAGA AGCAAGGATT 
AATTTTGACA AGAGGCAACA TGAAGCAAGA ATTCAGCAGT TAGAGAATGA GATTCACTAT 
TTACAAGAAA ATCTAAAAAG TATGGAGGAA ATCCAAGGGC TTACAGATCT GCAGCTGCAG 
GAAGCTGATG AAGAGAAGGA GAGAATTCTA GCCCAACTCC AGGAGTTAGA GAAGAAGAAG 
AAACTTGAAG ATGCCAAATC TCAGGCACAG GTTCTAGGTT TAGATAAAGA ATTGAAGAAA 
CTAAAGAAGG CTGTGGCCAC CTCTGATAAG ATAGCCACAG CTGAGCTCAC CATCGCCAAA 
GACCAGCTCA AGTCCCTTCA TGGAACTGTT ATGAAAATTA ACCAGGAGCG TGCAGAGGAC 
CTGCAGGAAG CAGAGCGGTT CAGCAGAAAG GCAGCACAGG CCGCGAGGGA TCTGACCCGA 
GCGGAAGCGG AGATCGAACT CCTGCAGAAC CTCCTCAGGG AGAGAGAGGA GCAGATGGAG 
AAGGTAGAGG CAGGCACCCG AGGAGCAAGC TCACAGGCGC TGGAAATCGA GAGGTGGAAT 
GAGACCATGG AGAGACAGAG GAGCGAGATC GCAAGGCTAC GGGACTCACT AGACCGCACC 
GGGGCGGATA ACAGAGGAGG CATTGAAAAT ATTTTGGAAG AAATAGCTGA ACTCCGACGT 
GAAGTTTCTT ACCAGAATGA TTACATCAGC AGCATGGCAG ATCCTTTCAA AAGACGAGGC 
TATTGGTACT TTATGCCACC ACCATCATCA TCAAAAGTTT CCAGCCACAG CTCCCAGGCC 
ACAAAGGACT CTGGTGTCGG GATGAAGTAC GCAGCCTCGA CTCCTATTAG AAAACCACAG 
CAGAACGGGA GGGAAGACGG TGCCCCTCCA GCTGCCTCCG GATACTGGGT GTATTCCCCC 
ATCAGGAGCG GCTTACATAA ATCGTTTTCA AATAAAGATG CAGACAGTGG AGGAGAGAGC 
CGGGAGGAGA GCGAGCTGGA TGACCACGAT GACCGCCCGT TCGTCCCTCC TCCTGGATAC 
ATGATGTACA CCGTGTTCCC CGACGGCTCT CCTGTACCAC AGGGCATGGC CCTCTACGCA 
CCGCCGCCTC CCTTGCCCAA CAATAGCCGA CCTCTCAGCC CTGGCACTGT TGTGTATGGC 
CCGCCCCCTG CTGGTGCCCC CATTGTCTAC GGGCCCCCAC CGCCCAGCTT CTCCGTCCCC 
CTCATCCCAG TGGGCGTGCT GCACTGCAAC GTCCCGGAGC ACCATGCCTT AGAGAATGAG 
GTTTCTAGAT TGGAAGACAT AATACAGCAT TTAAAATCAA AGAAGCAGGA ACAGAGGATG 
AGAGTATGCA AGCGGCAGTC TGAGAAAGAA ATAGAAGAAC TGCATCATAA TATTGATGAA 
CTTTTGCAAG AGAAGAAAGA CTTAGAGCAC GAAGTAGAAG AATTACATAG AACCATCCAG 
AAACATCAAC AGCAAAAAGA CTTCATTGAT GGAAATGTTG AGAGTCTTAT GAATGAACTA 
AAAATAGAAA AATCACTCAA GCACCATGAA GATATTGTAG ATGAAATTGA GTGCATCGAG 
AAGACTCTCC TGAAACGTCG AGCAGAGCTC AGGGAGGCTG ACCGACTGCT GGCGGAAGCC 
GAGAGTGAAC TTTCACGCAC AACAGAGAAA ACAAAAAATG CTGTTGAAAA GTTCACTGAT 
GCCAAGAGGA ATTTGTTGCA AACTGAGAAA GATGCTGAGG AGTTAGAAAG GAGAGCTCAG 
GAAACAGCTG TTAGCCTTGT CAAGGCTGAT CAACAGCTCA GATTGTTACA GGCGGACACA 
AAGGATTTGG AGCAGCACAA AACTGAGCAA GAAGAGATCT TGAAAGAGAT AAACAAAGTT 
GTTGCAGCAA AAGACTCAGA CTTCCAGTGT TTAAACACGA AGAAGGAGAA GCTAACAGAA 
GAGCTGCAGA AGCTGCAGAA AGACATCGAG ACGGCAGCGC GCACGGAGGA TCACCACCTG 
CAGGTCCTGA GAGAGTCGGA GGCCCTCCTG CAGGCCAAGA GAGCGGAGCT GGACAAGCTG 
AGGAGCCAGG TAACGAGTCA GCAGCAGGAG ATGGCTGTCT TGGACAGACA GCTGGGGCAT 
AAGAAGGAGG AGCTGCATCT ACTCCAGGGA AGCATGGTCC AGGCCAGAGC TGACCTCCAG 
GAAGCGCTGA GGCTGGGAGA AACTGAAGTA ACTGAGAAGT GCAACCACAT CAGGGAACTA 
AAAGGTCTTC TGGAAGAACT GAGTTTTCAG AAAGGAGAAC TGAATGTCCA GATTAGTGAG 
AAAAAAAATC AACTTTCACT TATAAAGCAA GAAATTGAAA AAGAGGAAGA AAATCTTCAG 
GTAGTTTTAG GGCAGATGTC TAAACATAAA ACTGAACTTA AGAATATTCT GGACATGTTG 
CAACTTGAAA ACAATGAGCT GCAAGCTTTG AAGCTACAGC ATGAGCAAAA GATGTCTGAG 
TTAGAGAAGG CTCAGGTTGA AGTGCTGGAG GAGAAACTGG AGTTGGAGAA TTTGCAGCAG 
ACAGCCCTGC GACAGAAAGG GGAGATCGAG TGGCAGAAGC AGCTCTTAGA GAGGGACAAG 
CGAGAGATTG AGCGGATAAC TGCTGAGACC CGAGCATTGC AGTTGTGTGT TGAGTCTTTG 
TGCAAACAAA AGGAAGATCT TGAGGAGAAA TGTGACAGCT GGGAGAAGAA GCTGGCACAG 
ACCAAACGGG TTTTAGCAGC CACAGAAGAG AATAGCAAAG CAAAGCAATC AGACTTAGAA 
AAGTTGGAAT TGGATATCAG AAAACTGGAA CAGGAACTAG ACCAGCTAAA CAGAAACAAA 
CTCTCACTGC ACAGAGACAT CGCGGCCATG CAGCAGCAGG TCCAAGAAAA ACGAGAAGCA 
GTAAACTCAT TACAGGAGGA ACTAGCTGAT GTCCAAGCCC ATTTGAACCT AGCGAAACAG 
GACCTGCTCC ACACCACCAA GCGCCAGGAT GTCCTGCTTG GCGAGCAGAC CAGGCTGCAG 
GAGGACCTCC AGGAACGCAG AAAGAAGTTG GAAGTCTGCC AAAAAGAAGA GGCAACAAAG 
CGGCAGCACC TGCAAGCGCT TCAGACGGAG ATGGAGGCGA ACGAGGCCGA GCTGGCCCGG 
CAGGAGACGA CATTTCAGAG ACTCCAGAAA GAGAGAGAAA GTGAAGAAAA AAAGTTAGAA 
GCCAGTCGAG CGACTCTGCA GGAGCAGCAG CAGCAGCTGG AAAAGGAAAT CGCAGACCAG 
AAGAACGTGC TGGACCAGGT GCTCAGCCAG GTGCTGGGGG CCAAGGAGCG CGCCAGGACT 
CTGCAGCAGG AGGAGAGGTG GGGTGAGACC CTGGAGAAGA CGCTCTCCCA GACCCAGCAA 
CAGCTCTCAG AACGGGAGCA GCAGTTGTTG GAAAAGTCAG CTGAGCTGCT GGCTCTTCAG 
AAAGAGGCAG ACTCGATGAG GGCAGACTTC AGCCTCTTGC GAAACCAGTT CTTGACGGAG 
AGGAAGAAAG CCGAGAAGCA GGTAGCCAGC CTCAGGGAAG CGCTCAAGAC CCAGCGCAGC 
CAGCTGGAGA AGACCCTTCT CGAGCAGAAG CAGGAGAACA GCTCCATGCA GAAGGAGATG 
GCGACTATCG AGCAGGTGGC GCAGGACAAC CACGAGCGGG CCCGGCGCCT GATGCGGGAG 
CTCAGCCAGA TGCAGCAGGA GTACGCGGAG CTCAAGAAGC AGATGGTAAA CCAGAAAGAT 
CTGGAAAGAA GACAAGTAGA AATCAGTGAA GCGATGAGGA CACTTAAGTC TGAAGTGAAA 
GACGAGATCA GAGCCAGCCT GAAGAACCTC AACCAGTTTC TCCCGGAGCT GCCAGCAGAC 
CTGGAGGCCA TTCTGGAAAG GAGCGAACAC CTAGACGGAG GGCTGGAGAG CTTGAAGGAG 
AACTTTCCAT TCGCCCTGAG TGAGAAGCCA TCACCTTTGG AAGAGAAACT ACATTTTTCT 
CGCGTTCACA TAATGGATGA GCACTGGCGT GGAGAAGCGC TCCGGGAGAA GCTGCGGCAC 
CGTGAGGACC GACTCAAGGC CCAGCTCCGA CACTGTATGT CCAAGCAGGC AGAGGTGTTA 
AGCAAAGGGA AGCGGCAGAC CGAGGGCACG TTACACAGCC TGAGGAGGCA GGTCGACGCC 
TTAGGGGAGC TGGTCACCAG CACGTCTGTG GAGTCCACGT CGTCAGCCAC TCTGTCTCAG 
CTCGAATCTT CCCTTGCAGA GGACTCCCAT CTTGGACCAA GTCAGAGCTC CTTCCATCAA 
GTTCCACAAG GTCCATCACA ATCAGTAGAC AGTTACCGAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CNTRL
Entrez Gene ID	102401063
Full Name	centriolin
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CNTRL cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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