USP4 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP4
Entrez Gene ID	102400841
Full Name	ubiquitin specific peptidase 4
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006053251.2	XP_006053313.2	ubiquitin carboxyl-terminal hydrolase 4 isoform X3
XM_006053250.1	XP_006053312.1	ubiquitin carboxyl-terminal hydrolase 4 isoform X1
XM_025272021.1	XP_025127806.1	ubiquitin carboxyl-terminal hydrolase 4 isoform X2
XM_025272022.1	XP_025127807.1	ubiquitin carboxyl-terminal hydrolase 4 isoform X4
XM_006053252.1	XP_006053314.1	ubiquitin carboxyl-terminal hydrolase 4 isoform X3
XM_006053251.1	XP_006053313.1	ubiquitin carboxyl-terminal hydrolase 4 isoform X2
XM_006053250.2	XP_006053312.2	ubiquitin carboxyl-terminal hydrolase 4 isoform X1
XM_006053252.2	XP_006053314.1	ubiquitin carboxyl-terminal hydrolase 4 isoform X5

Pathways from BioSystems
Gene Ontology

The following USP4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu10856	XM_006053250.1 Latest version!	Bubalus bubalis ubiquitin specific peptidase 4 (proto-oncogene) (USP4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu10857	XM_006053251.1 Latest version!	Bubalus bubalis ubiquitin specific peptidase 4 (proto-oncogene) (USP4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu10858	XM_006053252.2 Latest version!	Bubalus bubalis ubiquitin specific peptidase 4 (USP4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OBu57438	XM_006053250.2 Latest version!	Bubalus bubalis ubiquitin specific peptidase 4 (USP4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu57439	XM_025272021.1 Latest version!	Bubalus bubalis ubiquitin specific peptidase 4 (USP4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu57440	XM_006053251.2 Latest version!	Bubalus bubalis ubiquitin specific peptidase 4 (USP4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu57441	XM_025272022.1 Latest version!	Bubalus bubalis ubiquitin specific peptidase 4 (USP4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OBu10858	XM_006053252.1	Bubalus bubalis ubiquitin specific peptidase 4 (proto-oncogene) (USP4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP4 ( XM_006053250.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006053312.1 Bubalus bubalis ubiquitin specific peptidase 4 (proto-oncogene) (USP4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu10856
Clone ID Related Accession (Same CDS sequence)	XM_006053250.1
Accession Version	XM_006053250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	ubiquitin carboxyl-terminal hydrolase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784513.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG GCGGAGGGTA CCGTGAGCGA CCGGATGCGG AGACCCAGAA ATCCGAGCTC 
GGGGCCTTGA TGAGGACCAC GCTCCAGCGA GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTCGACAGCT GGGATATGTA CAATGTGGGT 
GAACATAATC TGTATCCTGG CCCGATAGAT AACTCCGGAC TCTTTTCAGA TCCTGAGAGT 
CAGACCTTAA AGGAACATTT AATTGATGAA TTGGACTATG TTTTGGTCCC AGCAGAGGCC 
TGGAATAAGT TACTAAACTG GTATGGCTGC GTAGAAGGCC AGCAGCCCAT TGTCAGAAAA 
GTGGTAGAAC ATGGCCTGTT TGTCAAGCAC TGCAAGGTGG AGGTATATTT GCTGGAACTG 
AAGCTTTGTG AGAACAGTGA CCCCACCAAC GTGCTGAGCT GCCATTTCAG CAAGGCAGAC 
ACCATCGCAA CCATCGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAG 
ACGCGGCTGT GGAACAAATA CATGAGCAAC ACCTACGAGC AGCTGAGCAA GCTGGACAAC 
ACTGTGCAGG ATGCCGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GATGGCACAT GGCCAAGGCA GACCCAGCAA TCCAAATCAA GCACTGCGCC TAGCAGAAAT 
TTTACTACCT CTCCAAAATC ATCAGCAAGT CCCTATTCCT CAGTGTCTGC CTCTCCCATT 
GCAAATGGTG ATAGCACTAA CACCTCGGGG ATGCACAGCT CCGGTGTCAG CAGGGGTGGA 
TCTGGCTTCT CTGCTTCGTA TAATTGTCAG GAGTCCCCAT TGACTCACGT CCAACCTGGG 
CTCTGCGGAC TCGGAAACCT GGGGAACACC TGCTTCATGA ACTCCGCTTT GCAGTGCTTA 
AGCAACACAG CACCGTTGAC CGACTACTTT CTCAAAGATG AATACGAAGC CGAAATCAAC 
AGGGACAACC CCCTGGGAAT GAAAGGGGAA ATTGCAGAGG CCTACGCAGA GCTCATTAAG 
CAGATGTGGT CTGGCAGGGA TGCTCATGTG GCACCTCGCA TGTTCAAAAC TCAGGTGGGA 
CGTTTTGCCC CTCAGTTTTC TGGCTACCAA CAACAAGACT CTCAGGAGCT TTTAGCCTTT 
CTTCTAGATG GATTGCATGA AGATTTGAAC CGTGTGAAGA AAAAACCCTA CCTAGAGCTG 
AAGGATGCCA ATGGGCGGCC AGATGCGGTG GTGGCAAAGG AAGCCTGGGA GAATCATAGG 
TTGAGGAATG ATTCTGTGAT CGTGGACACG TTCCATGGCC TCTTCAAATC TACATTGGTT 
TGCCCAGAAT GTGCTAAGGT TTCTGTGACC TTTGACCCAT TTTGCTATCT AACTCTCCCA 
CTGCCCTTGA AGAAAGACCG AGTTATGGAG ATCTTCCTAG TTCCTGCCGA CCCTCGCTGC 
AGGCCTACCC AGTACCGTGT GGTTGTGCCA CTGATGGGGG CCGTGTCTGA CCTGTGTGAG 
GCTCTCTCCA AACTGTCTGG CATTGCTGCA GAAAACATGG TGGTCACAGA TGTGTATAAT 
CACCGGTTCC ACAAGATTTT CCAAATGGAT GAAGGTTTAA ACCACATCAT GCCTCGGGAT 
GACATTTTTG TGTACGAAGT CTGCAGCACC TCCCCGGATG GCTCTGAGTG TGTCACGCTT 
CCCGTGTACT TCAGGGAGAG GAAATCTAGG CCGTCGAGCA CTTCTACCGG GGCAGTGCTG 
TACGGGCAGC CACTGCTGGT TTCTGTCCCC AAGCACAAGC TCACCCTCGA GTCTTTGTAC 
CAGGCGGTTT GTGAGCGCAT CAGCCGCTAT ATAAAACAGC CTTTGCCTGA TGAGTCAGGC 
AGCTCACCCT TGGAGCTGGG GGCCTGCAAT GGCTCCAGGA GCGGCTGTGC AGGTGAAGAT 
GAAGAAATGG AGCATCAGGA AGAAGGCAGA GAGCAGCTCT CAGAAACGGA GGGCAGTGGG 
GACGATGAGC CGGGAAGTGA CCACAGCGAG GCCACCCAAA AGAAGAACAA AGGCCGGCCT 
TGCCCCAGAA GGCTTTTTAC CTTCAGCCTT GTGAACTCCT ATGGCACAGC TGACATAAAT 
TCCCTTGCCA CTGATGGGAA ACTACTGAAA CTCAACTCTC GGTCCACACT AGCCATCGAC 
TGGGACAGTG AGACCCGGAG CCGTTACTTC AACGAACAGG AATCTGAGAC GTACGAGAAG 
CACGTCAGCA TGTTGCAACC TCAGAAGAAA AAGAAGACGG CGGTGGCCCT GAGGGACTGC 
ATCGAGCTCT TCACTACCAT GGAGACCCTT GGGGAACATG ACCCCTGGTA CTGTCCCAAC 
TGTAAGAAGC ACCAGCAGGC CACAAAGAAG TTTGACCTGT GGTCCTTGCC GAAGATCCTG 
GTGGTCCACC TCAAACGTTT CTCCTACAAT AGATACTGGA GGGATAAGCT TGATACAGTC 
GTGGAGTTCC CAGTCAGAGG TCTGAACATG TCGGAGTTTG TCTGTGACCC ATCAGCGAGG 
CCGTATGTGT ACGATCTCAT CGCTGTGTCC AATCACTATG GAGCCATGGG GGTTGGCCAC 
TACACTGCAT ACGCGAAGAA CAAACTGAAC GGGAAGTGGT ATTACTTTGA TGATAGCAAT 
GTGTCCCTGG CCTGTGAGGA TCAGATAGTG ACGAAAGCAG CTTATGTGCT GTTTTACCAG 
CGTCGAGACG ATGAGTTTCA TAAGACGCCG TCACTCAGTT TTCCTGGCTC TTCTGATGGA 
GGTGCAAGAC CAAGCAGCTC ACAGCAGGGC ACGGGGGATG ACGAGACTTA CAGCATGGAC 
ACCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006053312.1
CDS	69..2957
Translation	MAEGGGYRERPDAETQKSELGALMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLYPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPAEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTQQSKSSTAPSRNFTTSPKSSASPYSSVSASPIANGDSTNTSGMHSSGVSRGGSGFSASYNCQESPLTHVQPGLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRVMEIFLVPADPRCRPTQYRVVVPLMGAVSDLCEALSKLSGIAAENMVVTDVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSPDGSECVTLPVYFRERKSRPSSTSTGAVLYGQPLLVSVPKHKLTLESLYQAVCERISRYIKQPLPDESGSSPLELGACNGSRSGCAGEDEEMEHQEEGREQLSETEGSGDDEPGSDHSEATQKKNKGRPCPRRLFTFSLVNSYGTADINSLATDGKLLKLNSRSTLAIDWDSETRSRYFNEQESETYEKHVSMLQPQKKKKTAVALRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTVVEFPVRGLNMSEFVCDPSARPYVYDLIAVSNHYGAMGVGHYTAYAKNKLNGKWYYFDDSNVSLACEDQIVTKAAYVLFYQRRDDEFHKTPSLSFPGSSDGGARPSSSQQGTGDDETYSMDTN

Target ORF information:

RefSeq Version	XM_006053250.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ubiquitin specific peptidase 4 (proto-oncogene) (USP4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006053250.1

ORF Insert Sequence:

ATGGCGGAAG GCGGAGGGTA CCGTGAGCGA CCGGATGCGG AGACCCAGAA ATCCGAGCTC 
GGGGCCTTGA TGAGGACCAC GCTCCAGCGA GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTCGACAGCT GGGATATGTA CAATGTGGGT 
GAACATAATC TGTATCCTGG CCCGATAGAT AACTCCGGAC TCTTTTCAGA TCCTGAGAGT 
CAGACCTTAA AGGAACATTT AATTGATGAA TTGGACTATG TTTTGGTCCC AGCAGAGGCC 
TGGAATAAGT TACTAAACTG GTATGGCTGC GTAGAAGGCC AGCAGCCCAT TGTCAGAAAA 
GTGGTAGAAC ATGGCCTGTT TGTCAAGCAC TGCAAGGTGG AGGTATATTT GCTGGAACTG 
AAGCTTTGTG AGAACAGTGA CCCCACCAAC GTGCTGAGCT GCCATTTCAG CAAGGCAGAC 
ACCATCGCAA CCATCGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAG 
ACGCGGCTGT GGAACAAATA CATGAGCAAC ACCTACGAGC AGCTGAGCAA GCTGGACAAC 
ACTGTGCAGG ATGCCGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GATGGCACAT GGCCAAGGCA GACCCAGCAA TCCAAATCAA GCACTGCGCC TAGCAGAAAT 
TTTACTACCT CTCCAAAATC ATCAGCAAGT CCCTATTCCT CAGTGTCTGC CTCTCCCATT 
GCAAATGGTG ATAGCACTAA CACCTCGGGG ATGCACAGCT CCGGTGTCAG CAGGGGTGGA 
TCTGGCTTCT CTGCTTCGTA TAATTGTCAG GAGTCCCCAT TGACTCACGT CCAACCTGGG 
CTCTGCGGAC TCGGAAACCT GGGGAACACC TGCTTCATGA ACTCCGCTTT GCAGTGCTTA 
AGCAACACAG CACCGTTGAC CGACTACTTT CTCAAAGATG AATACGAAGC CGAAATCAAC 
AGGGACAACC CCCTGGGAAT GAAAGGGGAA ATTGCAGAGG CCTACGCAGA GCTCATTAAG 
CAGATGTGGT CTGGCAGGGA TGCTCATGTG GCACCTCGCA TGTTCAAAAC TCAGGTGGGA 
CGTTTTGCCC CTCAGTTTTC TGGCTACCAA CAACAAGACT CTCAGGAGCT TTTAGCCTTT 
CTTCTAGATG GATTGCATGA AGATTTGAAC CGTGTGAAGA AAAAACCCTA CCTAGAGCTG 
AAGGATGCCA ATGGGCGGCC AGATGCGGTG GTGGCAAAGG AAGCCTGGGA GAATCATAGG 
TTGAGGAATG ATTCTGTGAT CGTGGACACG TTCCATGGCC TCTTCAAATC TACATTGGTT 
TGCCCAGAAT GTGCTAAGGT TTCTGTGACC TTTGACCCAT TTTGCTATCT AACTCTCCCA 
CTGCCCTTGA AGAAAGACCG AGTTATGGAG ATCTTCCTAG TTCCTGCCGA CCCTCGCTGC 
AGGCCTACCC AGTACCGTGT GGTTGTGCCA CTGATGGGGG CCGTGTCTGA CCTGTGTGAG 
GCTCTCTCCA AACTGTCTGG CATTGCTGCA GAAAACATGG TGGTCACAGA TGTGTATAAT 
CACCGGTTCC ACAAGATTTT CCAAATGGAT GAAGGTTTAA ACCACATCAT GCCTCGGGAT 
GACATTTTTG TGTACGAAGT CTGCAGCACC TCCCCGGATG GCTCTGAGTG TGTCACGCTT 
CCCGTGTACT TCAGGGAGAG GAAATCTAGG CCGTCGAGCA CTTCTACCGG GGCAGTGCTG 
TACGGGCAGC CACTGCTGGT TTCTGTCCCC AAGCACAAGC TCACCCTCGA GTCTTTGTAC 
CAGGCGGTTT GTGAGCGCAT CAGCCGCTAT ATAAAACAGC CTTTGCCTGA TGAGTCAGGC 
AGCTCACCCT TGGAGCTGGG GGCCTGCAAT GGCTCCAGGA GCGGCTGTGC AGGTGAAGAT 
GAAGAAATGG AGCATCAGGA AGAAGGCAGA GAGCAGCTCT CAGAAACGGA GGGCAGTGGG 
GACGATGAGC CGGGAAGTGA CCACAGCGAG GCCACCCAAA AGAAGAACAA AGGCCGGCCT 
TGCCCCAGAA GGCTTTTTAC CTTCAGCCTT GTGAACTCCT ATGGCACAGC TGACATAAAT 
TCCCTTGCCA CTGATGGGAA ACTACTGAAA CTCAACTCTC GGTCCACACT AGCCATCGAC 
TGGGACAGTG AGACCCGGAG CCGTTACTTC AACGAACAGG AATCTGAGAC GTACGAGAAG 
CACGTCAGCA TGTTGCAACC TCAGAAGAAA AAGAAGACGG CGGTGGCCCT GAGGGACTGC 
ATCGAGCTCT TCACTACCAT GGAGACCCTT GGGGAACATG ACCCCTGGTA CTGTCCCAAC 
TGTAAGAAGC ACCAGCAGGC CACAAAGAAG TTTGACCTGT GGTCCTTGCC GAAGATCCTG 
GTGGTCCACC TCAAACGTTT CTCCTACAAT AGATACTGGA GGGATAAGCT TGATACAGTC 
GTGGAGTTCC CAGTCAGAGG TCTGAACATG TCGGAGTTTG TCTGTGACCC ATCAGCGAGG 
CCGTATGTGT ACGATCTCAT CGCTGTGTCC AATCACTATG GAGCCATGGG GGTTGGCCAC 
TACACTGCAT ACGCGAAGAA CAAACTGAAC GGGAAGTGGT ATTACTTTGA TGATAGCAAT 
GTGTCCCTGG CCTGTGAGGA TCAGATAGTG ACGAAAGCAG CTTATGTGCT GTTTTACCAG 
CGTCGAGACG ATGAGTTTCA TAAGACGCCG TCACTCAGTT TTCCTGGCTC TTCTGATGGA 
GGTGCAAGAC CAAGCAGCTC ACAGCAGGGC ACGGGGGATG ACGAGACTTA CAGCATGGAC 
ACCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP4 ( XM_006053251.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006053313.1 Bubalus bubalis ubiquitin specific peptidase 4 (proto-oncogene) (USP4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu10857
Clone ID Related Accession (Same CDS sequence)	XM_006053251.1
Accession Version	XM_006053251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	ubiquitin carboxyl-terminal hydrolase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784513.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG GCGGAGGGTA CCGTGAGCGA CCGGATGCGG AGACCCAGAA ATCCGAGCTC 
GGGGCCTTGA TGAGGACCAC GCTCCAGCGA GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTCGACAGCT GGGATATGTA CAATGTGGGT 
GAACATAATC TGTATCCTGG CCCGATAGAT AACTCCGGAC TCTTTTCAGA TCCTGAGAGT 
CAGACCTTAA AGGAACATTT AATTGATGAA TTGGACTATG TTTTGGTCCC AGCAGAGGCC 
TGGAATAAGT TACTAAACTG GTATGGCTGC GTAGAAGGCC AGCAGCCCAT TGTCAGAAAA 
GTGGTAGAAC ATGGCCTGTT TGTCAAGCAC TGCAAGGTGG AGGTATATTT GCTGGAACTG 
AAGCTTTGTG AGAACAGTGA CCCCACCAAC GTGCTGAGCT GCCATTTCAG CAAGGCAGAC 
ACCATCGCAA CCATCGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAG 
ACGCGGCTGT GGAACAAATA CATGAGCAAC ACCTACGAGC AGCTGAGCAA GCTGGACAAC 
ACTGTGCAGG ATGCCGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GATGGCACAT GGCCAAGGCA GACCCAGCAA TCCAATGGAT CTGGCTTCTC TGCTTCGTAT 
AATTGTCAGG AGTCCCCATT GACTCACGTC CAACCTGGGC TCTGCGGACT CGGAAACCTG 
GGGAACACCT GCTTCATGAA CTCCGCTTTG CAGTGCTTAA GCAACACAGC ACCGTTGACC 
GACTACTTTC TCAAAGATGA ATACGAAGCC GAAATCAACA GGGACAACCC CCTGGGAATG 
AAAGGGGAAA TTGCAGAGGC CTACGCAGAG CTCATTAAGC AGATGTGGTC TGGCAGGGAT 
GCTCATGTGG CACCTCGCAT GTTCAAAACT CAGGTGGGAC GTTTTGCCCC TCAGTTTTCT 
GGCTACCAAC AACAAGACTC TCAGGAGCTT TTAGCCTTTC TTCTAGATGG ATTGCATGAA 
GATTTGAACC GTGTGAAGAA AAAACCCTAC CTAGAGCTGA AGGATGCCAA TGGGCGGCCA 
GATGCGGTGG TGGCAAAGGA AGCCTGGGAG AATCATAGGT TGAGGAATGA TTCTGTGATC 
GTGGACACGT TCCATGGCCT CTTCAAATCT ACATTGGTTT GCCCAGAATG TGCTAAGGTT 
TCTGTGACCT TTGACCCATT TTGCTATCTA ACTCTCCCAC TGCCCTTGAA GAAAGACCGA 
GTTATGGAGA TCTTCCTAGT TCCTGCCGAC CCTCGCTGCA GGCCTACCCA GTACCGTGTG 
GTTGTGCCAC TGATGGGGGC CGTGTCTGAC CTGTGTGAGG CTCTCTCCAA ACTGTCTGGC 
ATTGCTGCAG AAAACATGGT GGTCACAGAT GTGTATAATC ACCGGTTCCA CAAGATTTTC 
CAAATGGATG AAGGTTTAAA CCACATCATG CCTCGGGATG ACATTTTTGT GTACGAAGTC 
TGCAGCACCT CCCCGGATGG CTCTGAGTGT GTCACGCTTC CCGTGTACTT CAGGGAGAGG 
AAATCTAGGC CGTCGAGCAC TTCTACCGGG GCAGTGCTGT ACGGGCAGCC ACTGCTGGTT 
TCTGTCCCCA AGCACAAGCT CACCCTCGAG TCTTTGTACC AGGCGGTTTG TGAGCGCATC 
AGCCGCTATA TAAAACAGCC TTTGCCTGAT GAGTCAGGCA GCTCACCCTT GGAGCTGGGG 
GCCTGCAATG GCTCCAGGAG CGGCTGTGCA GGTGAAGATG AAGAAATGGA GCATCAGGAA 
GAAGGCAGAG AGCAGCTCTC AGAAACGGAG GGCAGTGGGG ACGATGAGCC GGGAAGTGAC 
CACAGCGAGG CCACCCAAAA GAAGAACAAA GGCCGGCCTT GCCCCAGAAG GCTTTTTACC 
TTCAGCCTTG TGAACTCCTA TGGCACAGCT GACATAAATT CCCTTGCCAC TGATGGGAAA 
CTACTGAAAC TCAACTCTCG GTCCACACTA GCCATCGACT GGGACAGTGA GACCCGGAGC 
CGTTACTTCA ACGAACAGGA ATCTGAGACG TACGAGAAGC ACGTCAGCAT GTTGCAACCT 
CAGAAGAAAA AGAAGACGGC GGTGGCCCTG AGGGACTGCA TCGAGCTCTT CACTACCATG 
GAGACCCTTG GGGAACATGA CCCCTGGTAC TGTCCCAACT GTAAGAAGCA CCAGCAGGCC 
ACAAAGAAGT TTGACCTGTG GTCCTTGCCG AAGATCCTGG TGGTCCACCT CAAACGTTTC 
TCCTACAATA GATACTGGAG GGATAAGCTT GATACAGTCG TGGAGTTCCC AGTCAGAGGT 
CTGAACATGT CGGAGTTTGT CTGTGACCCA TCAGCGAGGC CGTATGTGTA CGATCTCATC 
GCTGTGTCCA ATCACTATGG AGCCATGGGG GTTGGCCACT ACACTGCATA CGCGAAGAAC 
AAACTGAACG GGAAGTGGTA TTACTTTGAT GATAGCAATG TGTCCCTGGC CTGTGAGGAT 
CAGATAGTGA CGAAAGCAGC TTATGTGCTG TTTTACCAGC GTCGAGACGA TGAGTTTCAT 
AAGACGCCGT CACTCAGTTT TCCTGGCTCT TCTGATGGAG GTGCAAGACC AAGCAGCTCA 
CAGCAGGGCA CGGGGGATGA CGAGACTTAC AGCATGGACA CCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006053313.1
CDS	69..2816
Translation	MAEGGGYRERPDAETQKSELGALMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLYPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPAEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTQQSNGSGFSASYNCQESPLTHVQPGLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRVMEIFLVPADPRCRPTQYRVVVPLMGAVSDLCEALSKLSGIAAENMVVTDVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSPDGSECVTLPVYFRERKSRPSSTSTGAVLYGQPLLVSVPKHKLTLESLYQAVCERISRYIKQPLPDESGSSPLELGACNGSRSGCAGEDEEMEHQEEGREQLSETEGSGDDEPGSDHSEATQKKNKGRPCPRRLFTFSLVNSYGTADINSLATDGKLLKLNSRSTLAIDWDSETRSRYFNEQESETYEKHVSMLQPQKKKKTAVALRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTVVEFPVRGLNMSEFVCDPSARPYVYDLIAVSNHYGAMGVGHYTAYAKNKLNGKWYYFDDSNVSLACEDQIVTKAAYVLFYQRRDDEFHKTPSLSFPGSSDGGARPSSSQQGTGDDETYSMDTN

Target ORF information:

RefSeq Version	XM_006053251.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ubiquitin specific peptidase 4 (proto-oncogene) (USP4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006053251.1

ORF Insert Sequence:

ATGGCGGAAG GCGGAGGGTA CCGTGAGCGA CCGGATGCGG AGACCCAGAA ATCCGAGCTC 
GGGGCCTTGA TGAGGACCAC GCTCCAGCGA GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTCGACAGCT GGGATATGTA CAATGTGGGT 
GAACATAATC TGTATCCTGG CCCGATAGAT AACTCCGGAC TCTTTTCAGA TCCTGAGAGT 
CAGACCTTAA AGGAACATTT AATTGATGAA TTGGACTATG TTTTGGTCCC AGCAGAGGCC 
TGGAATAAGT TACTAAACTG GTATGGCTGC GTAGAAGGCC AGCAGCCCAT TGTCAGAAAA 
GTGGTAGAAC ATGGCCTGTT TGTCAAGCAC TGCAAGGTGG AGGTATATTT GCTGGAACTG 
AAGCTTTGTG AGAACAGTGA CCCCACCAAC GTGCTGAGCT GCCATTTCAG CAAGGCAGAC 
ACCATCGCAA CCATCGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAG 
ACGCGGCTGT GGAACAAATA CATGAGCAAC ACCTACGAGC AGCTGAGCAA GCTGGACAAC 
ACTGTGCAGG ATGCCGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GATGGCACAT GGCCAAGGCA GACCCAGCAA TCCAATGGAT CTGGCTTCTC TGCTTCGTAT 
AATTGTCAGG AGTCCCCATT GACTCACGTC CAACCTGGGC TCTGCGGACT CGGAAACCTG 
GGGAACACCT GCTTCATGAA CTCCGCTTTG CAGTGCTTAA GCAACACAGC ACCGTTGACC 
GACTACTTTC TCAAAGATGA ATACGAAGCC GAAATCAACA GGGACAACCC CCTGGGAATG 
AAAGGGGAAA TTGCAGAGGC CTACGCAGAG CTCATTAAGC AGATGTGGTC TGGCAGGGAT 
GCTCATGTGG CACCTCGCAT GTTCAAAACT CAGGTGGGAC GTTTTGCCCC TCAGTTTTCT 
GGCTACCAAC AACAAGACTC TCAGGAGCTT TTAGCCTTTC TTCTAGATGG ATTGCATGAA 
GATTTGAACC GTGTGAAGAA AAAACCCTAC CTAGAGCTGA AGGATGCCAA TGGGCGGCCA 
GATGCGGTGG TGGCAAAGGA AGCCTGGGAG AATCATAGGT TGAGGAATGA TTCTGTGATC 
GTGGACACGT TCCATGGCCT CTTCAAATCT ACATTGGTTT GCCCAGAATG TGCTAAGGTT 
TCTGTGACCT TTGACCCATT TTGCTATCTA ACTCTCCCAC TGCCCTTGAA GAAAGACCGA 
GTTATGGAGA TCTTCCTAGT TCCTGCCGAC CCTCGCTGCA GGCCTACCCA GTACCGTGTG 
GTTGTGCCAC TGATGGGGGC CGTGTCTGAC CTGTGTGAGG CTCTCTCCAA ACTGTCTGGC 
ATTGCTGCAG AAAACATGGT GGTCACAGAT GTGTATAATC ACCGGTTCCA CAAGATTTTC 
CAAATGGATG AAGGTTTAAA CCACATCATG CCTCGGGATG ACATTTTTGT GTACGAAGTC 
TGCAGCACCT CCCCGGATGG CTCTGAGTGT GTCACGCTTC CCGTGTACTT CAGGGAGAGG 
AAATCTAGGC CGTCGAGCAC TTCTACCGGG GCAGTGCTGT ACGGGCAGCC ACTGCTGGTT 
TCTGTCCCCA AGCACAAGCT CACCCTCGAG TCTTTGTACC AGGCGGTTTG TGAGCGCATC 
AGCCGCTATA TAAAACAGCC TTTGCCTGAT GAGTCAGGCA GCTCACCCTT GGAGCTGGGG 
GCCTGCAATG GCTCCAGGAG CGGCTGTGCA GGTGAAGATG AAGAAATGGA GCATCAGGAA 
GAAGGCAGAG AGCAGCTCTC AGAAACGGAG GGCAGTGGGG ACGATGAGCC GGGAAGTGAC 
CACAGCGAGG CCACCCAAAA GAAGAACAAA GGCCGGCCTT GCCCCAGAAG GCTTTTTACC 
TTCAGCCTTG TGAACTCCTA TGGCACAGCT GACATAAATT CCCTTGCCAC TGATGGGAAA 
CTACTGAAAC TCAACTCTCG GTCCACACTA GCCATCGACT GGGACAGTGA GACCCGGAGC 
CGTTACTTCA ACGAACAGGA ATCTGAGACG TACGAGAAGC ACGTCAGCAT GTTGCAACCT 
CAGAAGAAAA AGAAGACGGC GGTGGCCCTG AGGGACTGCA TCGAGCTCTT CACTACCATG 
GAGACCCTTG GGGAACATGA CCCCTGGTAC TGTCCCAACT GTAAGAAGCA CCAGCAGGCC 
ACAAAGAAGT TTGACCTGTG GTCCTTGCCG AAGATCCTGG TGGTCCACCT CAAACGTTTC 
TCCTACAATA GATACTGGAG GGATAAGCTT GATACAGTCG TGGAGTTCCC AGTCAGAGGT 
CTGAACATGT CGGAGTTTGT CTGTGACCCA TCAGCGAGGC CGTATGTGTA CGATCTCATC 
GCTGTGTCCA ATCACTATGG AGCCATGGGG GTTGGCCACT ACACTGCATA CGCGAAGAAC 
AAACTGAACG GGAAGTGGTA TTACTTTGAT GATAGCAATG TGTCCCTGGC CTGTGAGGAT 
CAGATAGTGA CGAAAGCAGC TTATGTGCTG TTTTACCAGC GTCGAGACGA TGAGTTTCAT 
AAGACGCCGT CACTCAGTTT TCCTGGCTCT TCTGATGGAG GTGCAAGACC AAGCAGCTCA 
CAGCAGGGCA CGGGGGATGA CGAGACTTAC AGCATGGACA CCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP4 ( XM_006053252.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006053314.1 Bubalus bubalis ubiquitin specific peptidase 4 (proto-oncogene) (USP4), transcript variant X3, mRNA.

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CloneID	OBu10858
Clone ID Related Accession (Same CDS sequence)	XM_006053252.1 , XM_006053252.2
Accession Version	XM_006053252.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	ubiquitin carboxyl-terminal hydrolase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784513.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG GCGGAGGGTA CCGTGAGCGA CCGGATGCGG AGACCCAGAA ATCCGAGCTC 
GGGGCCTTGA TGAGGACCAC GCTCCAGCGA GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTCGACAGCT GGGATATGTA CAATGTGGGT 
GAACATAATC TGTATCCTGG CCCGATAGAT AACTCCGGAC TCTTTTCAGA TCCTGAGAGT 
CAGACCTTAA AGGAACATTT AATTGATGAA TTGGACTATG TTTTGGTCCC AGCAGAGGCC 
TGGAATAAGT TACTAAACTG GTATGGCTGC GTAGAAGGCC AGCAGCCCAT TGTCAGAAAA 
GTGGTAGAAC ATGGCCTGTT TGTCAAGCAC TGCAAGGTGG AGGTATATTT GCTGGAACTG 
AAGCTTTGTG AGAACAGTGA CCCCACCAAC GTGCTGAGCT GCCATTTCAG CAAGGCAGAC 
ACCATCGCAA CCATCGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAG 
ACGCGGCTGT GGAACAAATA CATGAGCAAC ACCTACGAGC AGCTGAGCAA GCTGGACAAC 
ACTGTGCAGG ATGCCGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GATGGCACAT GGCCAAGGCA GACCCAGCAA TCCAAATCAA GCACTGCGCC TAGCAGAAAT 
TTTACTACCT CTCCAAAATC ATCAGCAAGT CCCTATTCCT CAGTGTCTGC CTCTCCCATT 
GCAAATGGTG ATAGCACTAA CACCTCGGGG ATGCACAGCT CCGGTGTCAG CAGGGGTGGA 
TCTGGCTTCT CTGCTTCGTA TAATTGTCAG GAGTCCCCAT TGACTCACGT CCAACCTGGG 
CTCTGCGGAC TCGGAAACCT GGGGAACACC TGCTTCATGA ACTCCGCTTT GCAGTGCTTA 
AGCAACACAG CACCGTTGAC CGACTACTTT CTCAAAGATG AATACGAAGC CGAAATCAAC 
AGGGACAACC CCCTGGGAAT GAAAGGGGAA ATTGCAGAGG CCTACGCAGA GCTCATTAAG 
CAGATGTGGT CTGGCAGGGA TGCTCATGTG GCACCTCGCA TGTTCAAAAC TCAGGTGGGA 
CGTTTTGCCC CTCAGTTTTC TGGCTACCAA CAACAAGACT CTCAGGAGCT TTTAGCCTTT 
CTTCTAGATG GATTGCATGA AGATTTGAAC CGTGTGAAGA AAAAACCCTA CCTAGAGCTG 
AAGGATGCCA ATGGGCGGCC AGATGCGGTG GTGGCAAAGG AAGCCTGGGA GAATCATAGG 
TTGAGGAATG ATTCTGTGAT CGTGGACACG TTCCATGGCC TCTTCAAATC TACATTGGTT 
TGCCCAGAAT GTGCTAAGGT TTCTGTGACC TTTGACCCAT TTTGCTATCT AACTCTCCCA 
CTGCCCTTGA AGAAAGACCG AGTTATGGAG ATCTTCCTAG TTCCTGCCGA CCCTCGCTGC 
AGGCCTACCC AGTACCGTGT GGTTGTGCCA CTGATGGGGG CCGTGTCTGA CCTGTGTGAG 
GCTCTCTCCA AACTGTCTGG CATTGCTGCA GAAAACATGG TGGTCACAGA TGTGTATAAT 
CACCGGTTCC ACAAGATTTT CCAAATGGAT GAAGGTTTAA ACCACATCAT GCCTCGGGAT 
GACATTTTTG TGAGCACAGG CTGCTGCCCT ACTCCCAGCC ACCTTCCCGT ACTTCACAGA 
TGGAGGGGCC ACAGAGCTGG GACTGTTCAC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006053314.1
CDS	75..1850
Translation	MAEGGGYRERPDAETQKSELGALMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLYPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPAEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTQQSKSSTAPSRNFTTSPKSSASPYSSVSASPIANGDSTNTSGMHSSGVSRGGSGFSASYNCQESPLTHVQPGLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRVMEIFLVPADPRCRPTQYRVVVPLMGAVSDLCEALSKLSGIAAENMVVTDVYNHRFHKIFQMDEGLNHIMPRDDIFVSTGCCPTPSHLPVLHRWRGHRAGTVHF

Target ORF information:

RefSeq Version	XM_006053252.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ubiquitin specific peptidase 4 (proto-oncogene) (USP4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006053252.1

ORF Insert Sequence:

ATGGCGGAAG GCGGAGGGTA CCGTGAGCGA CCGGATGCGG AGACCCAGAA ATCCGAGCTC 
GGGGCCTTGA TGAGGACCAC GCTCCAGCGA GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTCGACAGCT GGGATATGTA CAATGTGGGT 
GAACATAATC TGTATCCTGG CCCGATAGAT AACTCCGGAC TCTTTTCAGA TCCTGAGAGT 
CAGACCTTAA AGGAACATTT AATTGATGAA TTGGACTATG TTTTGGTCCC AGCAGAGGCC 
TGGAATAAGT TACTAAACTG GTATGGCTGC GTAGAAGGCC AGCAGCCCAT TGTCAGAAAA 
GTGGTAGAAC ATGGCCTGTT TGTCAAGCAC TGCAAGGTGG AGGTATATTT GCTGGAACTG 
AAGCTTTGTG AGAACAGTGA CCCCACCAAC GTGCTGAGCT GCCATTTCAG CAAGGCAGAC 
ACCATCGCAA CCATCGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAG 
ACGCGGCTGT GGAACAAATA CATGAGCAAC ACCTACGAGC AGCTGAGCAA GCTGGACAAC 
ACTGTGCAGG ATGCCGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GATGGCACAT GGCCAAGGCA GACCCAGCAA TCCAAATCAA GCACTGCGCC TAGCAGAAAT 
TTTACTACCT CTCCAAAATC ATCAGCAAGT CCCTATTCCT CAGTGTCTGC CTCTCCCATT 
GCAAATGGTG ATAGCACTAA CACCTCGGGG ATGCACAGCT CCGGTGTCAG CAGGGGTGGA 
TCTGGCTTCT CTGCTTCGTA TAATTGTCAG GAGTCCCCAT TGACTCACGT CCAACCTGGG 
CTCTGCGGAC TCGGAAACCT GGGGAACACC TGCTTCATGA ACTCCGCTTT GCAGTGCTTA 
AGCAACACAG CACCGTTGAC CGACTACTTT CTCAAAGATG AATACGAAGC CGAAATCAAC 
AGGGACAACC CCCTGGGAAT GAAAGGGGAA ATTGCAGAGG CCTACGCAGA GCTCATTAAG 
CAGATGTGGT CTGGCAGGGA TGCTCATGTG GCACCTCGCA TGTTCAAAAC TCAGGTGGGA 
CGTTTTGCCC CTCAGTTTTC TGGCTACCAA CAACAAGACT CTCAGGAGCT TTTAGCCTTT 
CTTCTAGATG GATTGCATGA AGATTTGAAC CGTGTGAAGA AAAAACCCTA CCTAGAGCTG 
AAGGATGCCA ATGGGCGGCC AGATGCGGTG GTGGCAAAGG AAGCCTGGGA GAATCATAGG 
TTGAGGAATG ATTCTGTGAT CGTGGACACG TTCCATGGCC TCTTCAAATC TACATTGGTT 
TGCCCAGAAT GTGCTAAGGT TTCTGTGACC TTTGACCCAT TTTGCTATCT AACTCTCCCA 
CTGCCCTTGA AGAAAGACCG AGTTATGGAG ATCTTCCTAG TTCCTGCCGA CCCTCGCTGC 
AGGCCTACCC AGTACCGTGT GGTTGTGCCA CTGATGGGGG CCGTGTCTGA CCTGTGTGAG 
GCTCTCTCCA AACTGTCTGG CATTGCTGCA GAAAACATGG TGGTCACAGA TGTGTATAAT 
CACCGGTTCC ACAAGATTTT CCAAATGGAT GAAGGTTTAA ACCACATCAT GCCTCGGGAT 
GACATTTTTG TGAGCACAGG CTGCTGCCCT ACTCCCAGCC ACCTTCCCGT ACTTCACAGA 
TGGAGGGGCC ACAGAGCTGG GACTGTTCAC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP4 ( XM_006053252.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006053314.1 Bubalus bubalis ubiquitin specific peptidase 4 (USP4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu10858
Clone ID Related Accession (Same CDS sequence)	XM_006053252.1 , XM_006053252.2
Accession Version	XM_006053252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ubiquitin carboxyl-terminal hydrolase 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006053252.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG GCGGAGGGTA CCGTGAGCGA CCGGATGCGG AGACCCAGAA ATCCGAGCTC 
GGGGCCTTGA TGAGGACCAC GCTCCAGCGA GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTCGACAGCT GGGATATGTA CAATGTGGGT 
GAACATAATC TGTATCCTGG CCCGATAGAT AACTCCGGAC TCTTTTCAGA TCCTGAGAGT 
CAGACCTTAA AGGAACATTT AATTGATGAA TTGGACTATG TTTTGGTCCC AGCAGAGGCC 
TGGAATAAGT TACTAAACTG GTATGGCTGC GTAGAAGGCC AGCAGCCCAT TGTCAGAAAA 
GTGGTAGAAC ATGGCCTGTT TGTCAAGCAC TGCAAGGTGG AGGTATATTT GCTGGAACTG 
AAGCTTTGTG AGAACAGTGA CCCCACCAAC GTGCTGAGCT GCCATTTCAG CAAGGCAGAC 
ACCATCGCAA CCATCGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAG 
ACGCGGCTGT GGAACAAATA CATGAGCAAC ACCTACGAGC AGCTGAGCAA GCTGGACAAC 
ACTGTGCAGG ATGCCGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GATGGCACAT GGCCAAGGCA GACCCAGCAA TCCAAATCAA GCACTGCGCC TAGCAGAAAT 
TTTACTACCT CTCCAAAATC ATCAGCAAGT CCCTATTCCT CAGTGTCTGC CTCTCCCATT 
GCAAATGGTG ATAGCACTAA CACCTCGGGG ATGCACAGCT CCGGTGTCAG CAGGGGTGGA 
TCTGGCTTCT CTGCTTCGTA TAATTGTCAG GAGTCCCCAT TGACTCACGT CCAACCTGGG 
CTCTGCGGAC TCGGAAACCT GGGGAACACC TGCTTCATGA ACTCCGCTTT GCAGTGCTTA 
AGCAACACAG CACCGTTGAC CGACTACTTT CTCAAAGATG AATACGAAGC CGAAATCAAC 
AGGGACAACC CCCTGGGAAT GAAAGGGGAA ATTGCAGAGG CCTACGCAGA GCTCATTAAG 
CAGATGTGGT CTGGCAGGGA TGCTCATGTG GCACCTCGCA TGTTCAAAAC TCAGGTGGGA 
CGTTTTGCCC CTCAGTTTTC TGGCTACCAA CAACAAGACT CTCAGGAGCT TTTAGCCTTT 
CTTCTAGATG GATTGCATGA AGATTTGAAC CGTGTGAAGA AAAAACCCTA CCTAGAGCTG 
AAGGATGCCA ATGGGCGGCC AGATGCGGTG GTGGCAAAGG AAGCCTGGGA GAATCATAGG 
TTGAGGAATG ATTCTGTGAT CGTGGACACG TTCCATGGCC TCTTCAAATC TACATTGGTT 
TGCCCAGAAT GTGCTAAGGT TTCTGTGACC TTTGACCCAT TTTGCTATCT AACTCTCCCA 
CTGCCCTTGA AGAAAGACCG AGTTATGGAG ATCTTCCTAG TTCCTGCCGA CCCTCGCTGC 
AGGCCTACCC AGTACCGTGT GGTTGTGCCA CTGATGGGGG CCGTGTCTGA CCTGTGTGAG 
GCTCTCTCCA AACTGTCTGG CATTGCTGCA GAAAACATGG TGGTCACAGA TGTGTATAAT 
CACCGGTTCC ACAAGATTTT CCAAATGGAT GAAGGTTTAA ACCACATCAT GCCTCGGGAT 
GACATTTTTG TGAGCACAGG CTGCTGCCCT ACTCCCAGCC ACCTTCCCGT ACTTCACAGA 
TGGAGGGGCC ACAGAGCTGG GACTGTTCAC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006053314.1
CDS	71..1846
Translation	MAEGGGYRERPDAETQKSELGALMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLYPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPAEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTQQSKSSTAPSRNFTTSPKSSASPYSSVSASPIANGDSTNTSGMHSSGVSRGGSGFSASYNCQESPLTHVQPGLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRVMEIFLVPADPRCRPTQYRVVVPLMGAVSDLCEALSKLSGIAAENMVVTDVYNHRFHKIFQMDEGLNHIMPRDDIFVSTGCCPTPSHLPVLHRWRGHRAGTVHF

Target ORF information:

RefSeq Version	XM_006053252.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ubiquitin specific peptidase 4 (USP4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006053252.2

ORF Insert Sequence:

ATGGCGGAAG GCGGAGGGTA CCGTGAGCGA CCGGATGCGG AGACCCAGAA ATCCGAGCTC 
GGGGCCTTGA TGAGGACCAC GCTCCAGCGA GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTCGACAGCT GGGATATGTA CAATGTGGGT 
GAACATAATC TGTATCCTGG CCCGATAGAT AACTCCGGAC TCTTTTCAGA TCCTGAGAGT 
CAGACCTTAA AGGAACATTT AATTGATGAA TTGGACTATG TTTTGGTCCC AGCAGAGGCC 
TGGAATAAGT TACTAAACTG GTATGGCTGC GTAGAAGGCC AGCAGCCCAT TGTCAGAAAA 
GTGGTAGAAC ATGGCCTGTT TGTCAAGCAC TGCAAGGTGG AGGTATATTT GCTGGAACTG 
AAGCTTTGTG AGAACAGTGA CCCCACCAAC GTGCTGAGCT GCCATTTCAG CAAGGCAGAC 
ACCATCGCAA CCATCGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAG 
ACGCGGCTGT GGAACAAATA CATGAGCAAC ACCTACGAGC AGCTGAGCAA GCTGGACAAC 
ACTGTGCAGG ATGCCGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GATGGCACAT GGCCAAGGCA GACCCAGCAA TCCAAATCAA GCACTGCGCC TAGCAGAAAT 
TTTACTACCT CTCCAAAATC ATCAGCAAGT CCCTATTCCT CAGTGTCTGC CTCTCCCATT 
GCAAATGGTG ATAGCACTAA CACCTCGGGG ATGCACAGCT CCGGTGTCAG CAGGGGTGGA 
TCTGGCTTCT CTGCTTCGTA TAATTGTCAG GAGTCCCCAT TGACTCACGT CCAACCTGGG 
CTCTGCGGAC TCGGAAACCT GGGGAACACC TGCTTCATGA ACTCCGCTTT GCAGTGCTTA 
AGCAACACAG CACCGTTGAC CGACTACTTT CTCAAAGATG AATACGAAGC CGAAATCAAC 
AGGGACAACC CCCTGGGAAT GAAAGGGGAA ATTGCAGAGG CCTACGCAGA GCTCATTAAG 
CAGATGTGGT CTGGCAGGGA TGCTCATGTG GCACCTCGCA TGTTCAAAAC TCAGGTGGGA 
CGTTTTGCCC CTCAGTTTTC TGGCTACCAA CAACAAGACT CTCAGGAGCT TTTAGCCTTT 
CTTCTAGATG GATTGCATGA AGATTTGAAC CGTGTGAAGA AAAAACCCTA CCTAGAGCTG 
AAGGATGCCA ATGGGCGGCC AGATGCGGTG GTGGCAAAGG AAGCCTGGGA GAATCATAGG 
TTGAGGAATG ATTCTGTGAT CGTGGACACG TTCCATGGCC TCTTCAAATC TACATTGGTT 
TGCCCAGAAT GTGCTAAGGT TTCTGTGACC TTTGACCCAT TTTGCTATCT AACTCTCCCA 
CTGCCCTTGA AGAAAGACCG AGTTATGGAG ATCTTCCTAG TTCCTGCCGA CCCTCGCTGC 
AGGCCTACCC AGTACCGTGT GGTTGTGCCA CTGATGGGGG CCGTGTCTGA CCTGTGTGAG 
GCTCTCTCCA AACTGTCTGG CATTGCTGCA GAAAACATGG TGGTCACAGA TGTGTATAAT 
CACCGGTTCC ACAAGATTTT CCAAATGGAT GAAGGTTTAA ACCACATCAT GCCTCGGGAT 
GACATTTTTG TGAGCACAGG CTGCTGCCCT ACTCCCAGCC ACCTTCCCGT ACTTCACAGA 
TGGAGGGGCC ACAGAGCTGG GACTGTTCAC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP4 ( XM_006053250.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006053312.2 Bubalus bubalis ubiquitin specific peptidase 4 (USP4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu57438
Clone ID Related Accession (Same CDS sequence)	XM_006053250.2
Accession Version	XM_006053250.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ubiquitin carboxyl-terminal hydrolase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006053250.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG GCGGAGGGTA CCGTGAGCGA CCGGATGCGG AGACCCAGAA ATCCGAGCTC 
GGGGCCTTGA TGAGGACCAC GCTCCAGCGA GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTCGACAGCT GGGATATGTA CAATGTGGGT 
GAACATAATC TGTATCCTGG CCCGATAGAT AACTCCGGAC TCTTTTCAGA TCCTGAGAGT 
CAGACCTTAA AGGAACATTT AATTGATGAA TTGGACTATG TTTTGGTCCC AGCAGAGGCC 
TGGAATAAGT TACTAAACTG GTATGGCTGC GTAGAAGGCC AGCAGCCCAT TGTCAGAAAA 
GTGGTAGAAC ATGGCCTGTT TGTCAAGCAC TGCAAGGTGG AGGTATATTT GCTGGAACTG 
AAGCTTTGTG AGAACAGTGA CCCCACCAAC GTGCTGAGCT GCCATTTCAG CAAGGCAGAC 
ACCATCGCAA CCATCGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAG 
ACGCGGCTGT GGAACAAATA CATGAGCAAC ACCTACGAGC AGCTGAGCAA GCTGGACAAC 
ACTGTGCAGG ATGCCGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GATGGCACAT GGCCAAGGCA GACCCAGCAA TCCAAATCAA GCACTGCGCC TAGCAGAAAT 
TTTACTACCT CTCCAAAATC ATCAGCAAGT CCCTATTCCT CAGTGTCTGC CTCTCCCATT 
GCAAATGGTG ATAGCACTAA CACCTCGGGG ATGCACAGCT CCGGTGTCAG CAGGGGTGGA 
TCTGGCTTCT CTGCTTCGTA TAATTGTCAG GAGTCCCCAT TGACTCACGT CCAACCTGGG 
CTCTGCGGAC TCGGAAACCT GGGGAACACC TGCTTCATGA ACTCCGCTTT GCAGTGCTTA 
AGCAACACAG CACCGTTGAC CGACTACTTT CTCAAAGATG AATACGAAGC CGAAATCAAC 
AGGGACAACC CCCTGGGAAT GAAAGGGGAA ATTGCAGAGG CCTACGCAGA GCTCATTAAG 
CAGATGTGGT CTGGCAGGGA TGCTCATGTG GCACCTCGCA TGTTCAAAAC TCAGGTGGGA 
CGTTTTGCCC CTCAGTTTTC TGGCTACCAA CAACAAGACT CTCAGGAGCT TTTAGCCTTT 
CTTCTAGATG GATTGCATGA AGATTTGAAC CGTGTGAAGA AAAAACCCTA CCTAGAGCTG 
AAGGATGCCA ATGGGCGGCC AGATGCGGTG GTGGCAAAGG AAGCCTGGGA GAATCATAGG 
TTGAGGAATG ATTCTGTGAT CGTGGACACG TTCCATGGCC TCTTCAAATC TACATTGGTT 
TGCCCAGAAT GTGCTAAGGT TTCTGTGACC TTTGACCCAT TTTGCTATCT AACTCTCCCA 
CTGCCCTTGA AGAAAGACCG AGTTATGGAG ATCTTCCTAG TTCCTGCCGA CCCTCGCTGC 
AGGCCTACCC AGTACCGTGT GGTTGTGCCA CTGATGGGGG CCGTGTCTGA CCTGTGTGAG 
GCTCTCTCCA AACTGTCTGG CATTGCTGCA GAAAACATGG TGGTCACAGA TGTGTATAAT 
CACCGGTTCC ACAAGATTTT CCAAATGGAT GAAGGTTTAA ACCACATCAT GCCTCGGGAT 
GACATTTTTG TGTACGAAGT CTGCAGCACC TCCCCGGATG GCTCTGAGTG TGTCACGCTT 
CCCGTGTACT TCAGGGAGAG GAAATCTAGG CCGTCGAGCA CTTCTACCGG GGCAGTGCTG 
TACGGGCAGC CACTGCTGGT TTCTGTCCCC AAGCACAAGC TCACCCTCGA GTCTTTGTAC 
CAGGCGGTTT GTGAGCGCAT CAGCCGCTAT ATAAAACAGC CTTTGCCTGA TGAGTCAGGC 
AGCTCACCCT TGGAGCTGGG GGCCTGCAAT GGCTCCAGGA GCGGCTGTGC AGGTGAAGAT 
GAAGAAGAAA TGGAGCATCA GGAAGAAGGC AGAGAGCAGC TCTCAGAAAC AGAGGGCAGT 
GGGGACGATG AGCCGGGAAG TGACCACAGC GAGGCCACCC AAAAGAAGAA CAAAGGCCGG 
CCTTGCCCCA GAAGGCTTTT TACCTTCAGC CTTGTGAACT CCTATGGCAC AGCTGACATA 
AATTCCCTTG CCACTGATGG GAAACTACTG AAACTCAACT CTCGGTCCAC ACTAGCCATC 
GACTGGGACA GTGAGACCCG GAGCCGTTAC TTCAACGAAC AGGAATCTGA GACGTACGAG 
AAGCACGTCA GCATGTTGCA ACCTCAGAAG AAAAAGAAGA CGGCGGTGGC CCTGAGGGAC 
TGCATCGAGC TCTTCACTAC CATGGAGACC CTTGGGGAAC ATGACCCCTG GTACTGTCCC 
AACTGTAAGA AGCACCAGCA GGCCACAAAG AAGTTTGACC TGTGGTCCTT GCCGAAGATC 
CTGGTGGTCC ACCTCAAACG TTTCTCCTAC AATAGATACT GGAGGGATAA GCTTGATACA 
GTCGTGGAGT TCCCAGTCAG AGGTCTGAAC ATGTCGGAGT TTGTCTGTGA CCCATCAGCG 
AGGCCGTATG TGTACGATCT CATCGCTGTG TCCAATCACT ATGGAGCCAT GGGGGTTGGC 
CACTACACTG CATACGCGAA GAACAAACTG AACGGGAAGT GGTATTACTT TGATGATAGC 
AATGTGTCCC TGGCCTGTGA GGATCAGATA GTGACGAAAG CAGCTTATGT GCTGTTTTAC 
CAGCGTCGAG ACGATGAGTT TCATAAGACG CCGTCACTCA GTTTTCCTGG CTCTTCTGAT 
GGAGGTGCAA GACCAAGCAG CTCACAGCAG GGCACGGGGG ATGACGAGAC TTACAGCATG 
GACACCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006053312.2
CDS	68..2959
Translation	MAEGGGYRERPDAETQKSELGALMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLYPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPAEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTQQSKSSTAPSRNFTTSPKSSASPYSSVSASPIANGDSTNTSGMHSSGVSRGGSGFSASYNCQESPLTHVQPGLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRVMEIFLVPADPRCRPTQYRVVVPLMGAVSDLCEALSKLSGIAAENMVVTDVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSPDGSECVTLPVYFRERKSRPSSTSTGAVLYGQPLLVSVPKHKLTLESLYQAVCERISRYIKQPLPDESGSSPLELGACNGSRSGCAGEDEEEMEHQEEGREQLSETEGSGDDEPGSDHSEATQKKNKGRPCPRRLFTFSLVNSYGTADINSLATDGKLLKLNSRSTLAIDWDSETRSRYFNEQESETYEKHVSMLQPQKKKKTAVALRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTVVEFPVRGLNMSEFVCDPSARPYVYDLIAVSNHYGAMGVGHYTAYAKNKLNGKWYYFDDSNVSLACEDQIVTKAAYVLFYQRRDDEFHKTPSLSFPGSSDGGARPSSSQQGTGDDETYSMDTN

Target ORF information:

RefSeq Version	XM_006053250.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ubiquitin specific peptidase 4 (USP4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006053250.2

ORF Insert Sequence:

ATGGCGGAAG GCGGAGGGTA CCGTGAGCGA CCGGATGCGG AGACCCAGAA ATCCGAGCTC 
GGGGCCTTGA TGAGGACCAC GCTCCAGCGA GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTCGACAGCT GGGATATGTA CAATGTGGGT 
GAACATAATC TGTATCCTGG CCCGATAGAT AACTCCGGAC TCTTTTCAGA TCCTGAGAGT 
CAGACCTTAA AGGAACATTT AATTGATGAA TTGGACTATG TTTTGGTCCC AGCAGAGGCC 
TGGAATAAGT TACTAAACTG GTATGGCTGC GTAGAAGGCC AGCAGCCCAT TGTCAGAAAA 
GTGGTAGAAC ATGGCCTGTT TGTCAAGCAC TGCAAGGTGG AGGTATATTT GCTGGAACTG 
AAGCTTTGTG AGAACAGTGA CCCCACCAAC GTGCTGAGCT GCCATTTCAG CAAGGCAGAC 
ACCATCGCAA CCATCGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAG 
ACGCGGCTGT GGAACAAATA CATGAGCAAC ACCTACGAGC AGCTGAGCAA GCTGGACAAC 
ACTGTGCAGG ATGCCGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GATGGCACAT GGCCAAGGCA GACCCAGCAA TCCAAATCAA GCACTGCGCC TAGCAGAAAT 
TTTACTACCT CTCCAAAATC ATCAGCAAGT CCCTATTCCT CAGTGTCTGC CTCTCCCATT 
GCAAATGGTG ATAGCACTAA CACCTCGGGG ATGCACAGCT CCGGTGTCAG CAGGGGTGGA 
TCTGGCTTCT CTGCTTCGTA TAATTGTCAG GAGTCCCCAT TGACTCACGT CCAACCTGGG 
CTCTGCGGAC TCGGAAACCT GGGGAACACC TGCTTCATGA ACTCCGCTTT GCAGTGCTTA 
AGCAACACAG CACCGTTGAC CGACTACTTT CTCAAAGATG AATACGAAGC CGAAATCAAC 
AGGGACAACC CCCTGGGAAT GAAAGGGGAA ATTGCAGAGG CCTACGCAGA GCTCATTAAG 
CAGATGTGGT CTGGCAGGGA TGCTCATGTG GCACCTCGCA TGTTCAAAAC TCAGGTGGGA 
CGTTTTGCCC CTCAGTTTTC TGGCTACCAA CAACAAGACT CTCAGGAGCT TTTAGCCTTT 
CTTCTAGATG GATTGCATGA AGATTTGAAC CGTGTGAAGA AAAAACCCTA CCTAGAGCTG 
AAGGATGCCA ATGGGCGGCC AGATGCGGTG GTGGCAAAGG AAGCCTGGGA GAATCATAGG 
TTGAGGAATG ATTCTGTGAT CGTGGACACG TTCCATGGCC TCTTCAAATC TACATTGGTT 
TGCCCAGAAT GTGCTAAGGT TTCTGTGACC TTTGACCCAT TTTGCTATCT AACTCTCCCA 
CTGCCCTTGA AGAAAGACCG AGTTATGGAG ATCTTCCTAG TTCCTGCCGA CCCTCGCTGC 
AGGCCTACCC AGTACCGTGT GGTTGTGCCA CTGATGGGGG CCGTGTCTGA CCTGTGTGAG 
GCTCTCTCCA AACTGTCTGG CATTGCTGCA GAAAACATGG TGGTCACAGA TGTGTATAAT 
CACCGGTTCC ACAAGATTTT CCAAATGGAT GAAGGTTTAA ACCACATCAT GCCTCGGGAT 
GACATTTTTG TGTACGAAGT CTGCAGCACC TCCCCGGATG GCTCTGAGTG TGTCACGCTT 
CCCGTGTACT TCAGGGAGAG GAAATCTAGG CCGTCGAGCA CTTCTACCGG GGCAGTGCTG 
TACGGGCAGC CACTGCTGGT TTCTGTCCCC AAGCACAAGC TCACCCTCGA GTCTTTGTAC 
CAGGCGGTTT GTGAGCGCAT CAGCCGCTAT ATAAAACAGC CTTTGCCTGA TGAGTCAGGC 
AGCTCACCCT TGGAGCTGGG GGCCTGCAAT GGCTCCAGGA GCGGCTGTGC AGGTGAAGAT 
GAAGAAGAAA TGGAGCATCA GGAAGAAGGC AGAGAGCAGC TCTCAGAAAC AGAGGGCAGT 
GGGGACGATG AGCCGGGAAG TGACCACAGC GAGGCCACCC AAAAGAAGAA CAAAGGCCGG 
CCTTGCCCCA GAAGGCTTTT TACCTTCAGC CTTGTGAACT CCTATGGCAC AGCTGACATA 
AATTCCCTTG CCACTGATGG GAAACTACTG AAACTCAACT CTCGGTCCAC ACTAGCCATC 
GACTGGGACA GTGAGACCCG GAGCCGTTAC TTCAACGAAC AGGAATCTGA GACGTACGAG 
AAGCACGTCA GCATGTTGCA ACCTCAGAAG AAAAAGAAGA CGGCGGTGGC CCTGAGGGAC 
TGCATCGAGC TCTTCACTAC CATGGAGACC CTTGGGGAAC ATGACCCCTG GTACTGTCCC 
AACTGTAAGA AGCACCAGCA GGCCACAAAG AAGTTTGACC TGTGGTCCTT GCCGAAGATC 
CTGGTGGTCC ACCTCAAACG TTTCTCCTAC AATAGATACT GGAGGGATAA GCTTGATACA 
GTCGTGGAGT TCCCAGTCAG AGGTCTGAAC ATGTCGGAGT TTGTCTGTGA CCCATCAGCG 
AGGCCGTATG TGTACGATCT CATCGCTGTG TCCAATCACT ATGGAGCCAT GGGGGTTGGC 
CACTACACTG CATACGCGAA GAACAAACTG AACGGGAAGT GGTATTACTT TGATGATAGC 
AATGTGTCCC TGGCCTGTGA GGATCAGATA GTGACGAAAG CAGCTTATGT GCTGTTTTAC 
CAGCGTCGAG ACGATGAGTT TCATAAGACG CCGTCACTCA GTTTTCCTGG CTCTTCTGAT 
GGAGGTGCAA GACCAAGCAG CTCACAGCAG GGCACGGGGG ATGACGAGAC TTACAGCATG 
GACACCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP4 ( XM_025272021.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025127806.1 Bubalus bubalis ubiquitin specific peptidase 4 (USP4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu57439
Clone ID Related Accession (Same CDS sequence)	XM_025272021.1
Accession Version	XM_025272021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ubiquitin carboxyl-terminal hydrolase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG GCGGAGGGTA CCGTGAGCGA CCGGATGCGG AGACCCAGAA ATCCGAGCTC 
GGGGCCTTGA TGAGGACCAC GCTCCAGCGA GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTCGACAGCT GGGATATGTA CAATGTGGGT 
GAACATAATC TGTATCCTGG CCCGATAGAT AACTCCGGAC TCTTTTCAGA TCCTGAGAGT 
CAGACCTTAA AGGAACATTT AATTGATGAA TTGGACTATG TTTTGGTCCC AGCAGAGGCC 
TGGAATAAGT TACTAAACTG GTATGGCTGC GTAGAAGGCC AGCAGCCCAT TGTCAGAAAA 
GTGGTAGAAC ATGGCCTGTT TGTCAAGCAC TGCAAGGTGG AGGTATATTT GCTGGAACTG 
AAGCTTTGTG AGAACAGTGA CCCCACCAAC GTGCTGAGCT GCCATTTCAG CAAGGCAGAC 
ACCATCGCAA CCATCGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAG 
ACGCGGCTGT GGAACAAATA CATGAGCAAC ACCTACGAGC AGCTGAGCAA GCTGGACAAC 
ACTGTGCAGG ATGCCGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GATGGCACAT GGCCAAGGCA GACCCAGCAA TCCAAATCAA GCACTGCGCC TAGCAGAAAT 
TTTACTACCT CTCCAAAATC ATCAGCAAGT CCCTATTCCT CAGTGTCTGC CTCTCCCATT 
GCAAATGGTG ATAGCACTAA CACCTCGGGG ATGCACAGCT CCGGTGTCAG CAGGGGTGGA 
TCTGGCTTCT CTGCTTCGTA TAATTGTCAG GAGTCCCCAT TGACTCACGT CCAACCTGGG 
CTCTGCGGAC TCGGAAACCT GGGGAACACC TGCTTCATGA ACTCCGCTTT GCAGTGCTTA 
AGCAACACAG CACCGTTGAC CGACTACTTT CTCAAAGATG AATACGAAGC CGAAATCAAC 
AGGGACAACC CCCTGGGAAT GAAAGGGGAA ATTGCAGAGG CCTACGCAGA GCTCATTAAG 
CAGATGTGGT CTGGCAGGGA TGCTCATGTG GCACCTCGCA TGTTCAAAAC TCAGGTGGGA 
CGTTTTGCCC CTCAGTTTTC TGGCTACCAA CAACAAGACT CTCAGGAGCT TTTAGCCTTT 
CTTCTAGATG GATTGCATGA AGATTTGAAC CGTGTGAAGA AAAAACCCTA CCTAGAGCTG 
AAGGATGCCA ATGGGCGGCC AGATGCGGTG GTGGCAAAGG AAGCCTGGGA GAATCATAGG 
TTGAGGAATG ATTCTGTGAT CGTGGACACG TTCCATGGCC TCTTCAAATC TACATTGGTT 
TGCCCAGAAT GTGCTAAGGT TTCTGTGACC TTTGACCCAT TTTGCTATCT AACTCTCCCA 
CTGCCCTTGA AGAAAGACCG AGTTATGGAG ATCTTCCTAG TTCCTGCCGA CCCTCGCTGC 
AGGCCTACCC AGTACCGTGT GGTTGTGCCA CTGATGGGGG CCGTGTCTGA CCTGTGTGAG 
GCTCTCTCCA AACTGTCTGG CATTGCTGCA GAAAACATGG TGGTCACAGA TGTGTATAAT 
CACCGGTTCC ACAAGATTTT CCAAATGGAT GAAGGTTTAA ACCACATCAT GCCTCGGGAT 
GACATTTTTG TGTACGAAGT CTGCAGCACC TCCCCGGATG GCTCTGAGTG TGTCACGCTT 
CCCGTGTACT TCAGGGAGAG GAAATCTAGG CCGTCGAGCA CTTCTACCGG GGCAGTGCTG 
TACGGGCAGC CACTGCTGGT TTCTGTCCCC AAGCACAAGC TCACCCTCGA GTCTTTGTAC 
CAGGCGGTTT GTGAGCGCAT CAGCCGCTAT ATAAAACAGC CTTTGCCTGA TGAGTCAGGC 
AGCTCACCCT TGGAGCTGGG GGCCTGCAAT GGCTCCAGGA GCGGCTGTGC AGGTGAAGAT 
GAAGAAGAAA TGGAGCATCA GGAAGAAGGC AGAGAGCAGC TCTCAGAAAC AGAGGGCAGT 
GGGGACGATG AGCCGGGAAG TGACCACAGC GAGGCCACCC AAAAGAAGAA CAAAGGCCGG 
CCTTGCCCCA GAAGGCTTTT TACCTTCAGC CTTGTGAACT CCTATGGCAC AGCTGACATA 
AATTCCCTTG CCACTGATGG GAAACTACTG AAACTCAACT CTCGGTCCAC ACTAGCCATC 
GACTGGGACA GTGAGACCCG GAGCCGTTAC TTCAACGAAC AGGAATCTGA GACGTACGAG 
AAGCACGTCA GCATGTTGCA ACCTCAGAAG AAAAAGAAGA CGGCGGTGGC CCTGAGGGAC 
TGCATCGAGC TCTTCACTAC CATGGAGACC CTTGGGGAAC ATGACCCCTG GTACTGTCCC 
AACTGTAAGA AGCACCAGCA GGCCACAAAG AAGTTTGACC TGTGGTCCTT GCCGAAGATC 
CTGGTGGTCC ACCTCAAACG TTTCTCCTAC AATAGATACT GGAGGGATAA GCTTGATACA 
GTCGTGGAGT TCCCAGTCAG AGGTCTGAAC ATGTCGGAGT TTGTCTGTGA CCCATCAGCG 
AGGCCGTATG TGTACGATCT CATCGCTGTG TCCAATCACT ATGGAGCCAT GGGGGTTGGC 
CACTGTGTTA GTGATGTTGA AGTAAGGAGC TGGGTCTCCT CACGTCTCCT CTGTTGTATC 
TGCACCATGA GAACTGCCAC TACACCCCGT GTCAGGACCC CAAGAGATGA CACCATGGGA 
GGTGTCGGTT CCATTTACCC TCACAGTGGA TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025127806.1
CDS	70..2865
Translation	MAEGGGYRERPDAETQKSELGALMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLYPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPAEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTQQSKSSTAPSRNFTTSPKSSASPYSSVSASPIANGDSTNTSGMHSSGVSRGGSGFSASYNCQESPLTHVQPGLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRVMEIFLVPADPRCRPTQYRVVVPLMGAVSDLCEALSKLSGIAAENMVVTDVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSPDGSECVTLPVYFRERKSRPSSTSTGAVLYGQPLLVSVPKHKLTLESLYQAVCERISRYIKQPLPDESGSSPLELGACNGSRSGCAGEDEEEMEHQEEGREQLSETEGSGDDEPGSDHSEATQKKNKGRPCPRRLFTFSLVNSYGTADINSLATDGKLLKLNSRSTLAIDWDSETRSRYFNEQESETYEKHVSMLQPQKKKKTAVALRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTVVEFPVRGLNMSEFVCDPSARPYVYDLIAVSNHYGAMGVGHCVSDVEVRSWVSSRLLCCICTMRTATTPRVRTPRDDTMGGVGSIYPHSGL

Target ORF information:

RefSeq Version	XM_025272021.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ubiquitin specific peptidase 4 (USP4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025272021.1

ORF Insert Sequence:

ATGGCGGAAG GCGGAGGGTA CCGTGAGCGA CCGGATGCGG AGACCCAGAA ATCCGAGCTC 
GGGGCCTTGA TGAGGACCAC GCTCCAGCGA GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTCGACAGCT GGGATATGTA CAATGTGGGT 
GAACATAATC TGTATCCTGG CCCGATAGAT AACTCCGGAC TCTTTTCAGA TCCTGAGAGT 
CAGACCTTAA AGGAACATTT AATTGATGAA TTGGACTATG TTTTGGTCCC AGCAGAGGCC 
TGGAATAAGT TACTAAACTG GTATGGCTGC GTAGAAGGCC AGCAGCCCAT TGTCAGAAAA 
GTGGTAGAAC ATGGCCTGTT TGTCAAGCAC TGCAAGGTGG AGGTATATTT GCTGGAACTG 
AAGCTTTGTG AGAACAGTGA CCCCACCAAC GTGCTGAGCT GCCATTTCAG CAAGGCAGAC 
ACCATCGCAA CCATCGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAG 
ACGCGGCTGT GGAACAAATA CATGAGCAAC ACCTACGAGC AGCTGAGCAA GCTGGACAAC 
ACTGTGCAGG ATGCCGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GATGGCACAT GGCCAAGGCA GACCCAGCAA TCCAAATCAA GCACTGCGCC TAGCAGAAAT 
TTTACTACCT CTCCAAAATC ATCAGCAAGT CCCTATTCCT CAGTGTCTGC CTCTCCCATT 
GCAAATGGTG ATAGCACTAA CACCTCGGGG ATGCACAGCT CCGGTGTCAG CAGGGGTGGA 
TCTGGCTTCT CTGCTTCGTA TAATTGTCAG GAGTCCCCAT TGACTCACGT CCAACCTGGG 
CTCTGCGGAC TCGGAAACCT GGGGAACACC TGCTTCATGA ACTCCGCTTT GCAGTGCTTA 
AGCAACACAG CACCGTTGAC CGACTACTTT CTCAAAGATG AATACGAAGC CGAAATCAAC 
AGGGACAACC CCCTGGGAAT GAAAGGGGAA ATTGCAGAGG CCTACGCAGA GCTCATTAAG 
CAGATGTGGT CTGGCAGGGA TGCTCATGTG GCACCTCGCA TGTTCAAAAC TCAGGTGGGA 
CGTTTTGCCC CTCAGTTTTC TGGCTACCAA CAACAAGACT CTCAGGAGCT TTTAGCCTTT 
CTTCTAGATG GATTGCATGA AGATTTGAAC CGTGTGAAGA AAAAACCCTA CCTAGAGCTG 
AAGGATGCCA ATGGGCGGCC AGATGCGGTG GTGGCAAAGG AAGCCTGGGA GAATCATAGG 
TTGAGGAATG ATTCTGTGAT CGTGGACACG TTCCATGGCC TCTTCAAATC TACATTGGTT 
TGCCCAGAAT GTGCTAAGGT TTCTGTGACC TTTGACCCAT TTTGCTATCT AACTCTCCCA 
CTGCCCTTGA AGAAAGACCG AGTTATGGAG ATCTTCCTAG TTCCTGCCGA CCCTCGCTGC 
AGGCCTACCC AGTACCGTGT GGTTGTGCCA CTGATGGGGG CCGTGTCTGA CCTGTGTGAG 
GCTCTCTCCA AACTGTCTGG CATTGCTGCA GAAAACATGG TGGTCACAGA TGTGTATAAT 
CACCGGTTCC ACAAGATTTT CCAAATGGAT GAAGGTTTAA ACCACATCAT GCCTCGGGAT 
GACATTTTTG TGTACGAAGT CTGCAGCACC TCCCCGGATG GCTCTGAGTG TGTCACGCTT 
CCCGTGTACT TCAGGGAGAG GAAATCTAGG CCGTCGAGCA CTTCTACCGG GGCAGTGCTG 
TACGGGCAGC CACTGCTGGT TTCTGTCCCC AAGCACAAGC TCACCCTCGA GTCTTTGTAC 
CAGGCGGTTT GTGAGCGCAT CAGCCGCTAT ATAAAACAGC CTTTGCCTGA TGAGTCAGGC 
AGCTCACCCT TGGAGCTGGG GGCCTGCAAT GGCTCCAGGA GCGGCTGTGC AGGTGAAGAT 
GAAGAAGAAA TGGAGCATCA GGAAGAAGGC AGAGAGCAGC TCTCAGAAAC AGAGGGCAGT 
GGGGACGATG AGCCGGGAAG TGACCACAGC GAGGCCACCC AAAAGAAGAA CAAAGGCCGG 
CCTTGCCCCA GAAGGCTTTT TACCTTCAGC CTTGTGAACT CCTATGGCAC AGCTGACATA 
AATTCCCTTG CCACTGATGG GAAACTACTG AAACTCAACT CTCGGTCCAC ACTAGCCATC 
GACTGGGACA GTGAGACCCG GAGCCGTTAC TTCAACGAAC AGGAATCTGA GACGTACGAG 
AAGCACGTCA GCATGTTGCA ACCTCAGAAG AAAAAGAAGA CGGCGGTGGC CCTGAGGGAC 
TGCATCGAGC TCTTCACTAC CATGGAGACC CTTGGGGAAC ATGACCCCTG GTACTGTCCC 
AACTGTAAGA AGCACCAGCA GGCCACAAAG AAGTTTGACC TGTGGTCCTT GCCGAAGATC 
CTGGTGGTCC ACCTCAAACG TTTCTCCTAC AATAGATACT GGAGGGATAA GCTTGATACA 
GTCGTGGAGT TCCCAGTCAG AGGTCTGAAC ATGTCGGAGT TTGTCTGTGA CCCATCAGCG 
AGGCCGTATG TGTACGATCT CATCGCTGTG TCCAATCACT ATGGAGCCAT GGGGGTTGGC 
CACTGTGTTA GTGATGTTGA AGTAAGGAGC TGGGTCTCCT CACGTCTCCT CTGTTGTATC 
TGCACCATGA GAACTGCCAC TACACCCCGT GTCAGGACCC CAAGAGATGA CACCATGGGA 
GGTGTCGGTT CCATTTACCC TCACAGTGGA TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP4 ( XM_006053251.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006053313.2 Bubalus bubalis ubiquitin specific peptidase 4 (USP4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu57440
Clone ID Related Accession (Same CDS sequence)	XM_006053251.2
Accession Version	XM_006053251.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ubiquitin carboxyl-terminal hydrolase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006053251.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG GCGGAGGGTA CCGTGAGCGA CCGGATGCGG AGACCCAGAA ATCCGAGCTC 
GGGGCCTTGA TGAGGACCAC GCTCCAGCGA GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTCGACAGCT GGGATATGTA CAATGTGGGT 
GAACATAATC TGTATCCTGG CCCGATAGAT AACTCCGGAC TCTTTTCAGA TCCTGAGAGT 
CAGACCTTAA AGGAACATTT AATTGATGAA TTGGACTATG TTTTGGTCCC AGCAGAGGCC 
TGGAATAAGT TACTAAACTG GTATGGCTGC GTAGAAGGCC AGCAGCCCAT TGTCAGAAAA 
GTGGTAGAAC ATGGCCTGTT TGTCAAGCAC TGCAAGGTGG AGGTATATTT GCTGGAACTG 
AAGCTTTGTG AGAACAGTGA CCCCACCAAC GTGCTGAGCT GCCATTTCAG CAAGGCAGAC 
ACCATCGCAA CCATCGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAG 
ACGCGGCTGT GGAACAAATA CATGAGCAAC ACCTACGAGC AGCTGAGCAA GCTGGACAAC 
ACTGTGCAGG ATGCCGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GATGGCACAT GGCCAAGGCA GACCCAGCAA TCCAATGGAT CTGGCTTCTC TGCTTCGTAT 
AATTGTCAGG AGTCCCCATT GACTCACGTC CAACCTGGGC TCTGCGGACT CGGAAACCTG 
GGGAACACCT GCTTCATGAA CTCCGCTTTG CAGTGCTTAA GCAACACAGC ACCGTTGACC 
GACTACTTTC TCAAAGATGA ATACGAAGCC GAAATCAACA GGGACAACCC CCTGGGAATG 
AAAGGGGAAA TTGCAGAGGC CTACGCAGAG CTCATTAAGC AGATGTGGTC TGGCAGGGAT 
GCTCATGTGG CACCTCGCAT GTTCAAAACT CAGGTGGGAC GTTTTGCCCC TCAGTTTTCT 
GGCTACCAAC AACAAGACTC TCAGGAGCTT TTAGCCTTTC TTCTAGATGG ATTGCATGAA 
GATTTGAACC GTGTGAAGAA AAAACCCTAC CTAGAGCTGA AGGATGCCAA TGGGCGGCCA 
GATGCGGTGG TGGCAAAGGA AGCCTGGGAG AATCATAGGT TGAGGAATGA TTCTGTGATC 
GTGGACACGT TCCATGGCCT CTTCAAATCT ACATTGGTTT GCCCAGAATG TGCTAAGGTT 
TCTGTGACCT TTGACCCATT TTGCTATCTA ACTCTCCCAC TGCCCTTGAA GAAAGACCGA 
GTTATGGAGA TCTTCCTAGT TCCTGCCGAC CCTCGCTGCA GGCCTACCCA GTACCGTGTG 
GTTGTGCCAC TGATGGGGGC CGTGTCTGAC CTGTGTGAGG CTCTCTCCAA ACTGTCTGGC 
ATTGCTGCAG AAAACATGGT GGTCACAGAT GTGTATAATC ACCGGTTCCA CAAGATTTTC 
CAAATGGATG AAGGTTTAAA CCACATCATG CCTCGGGATG ACATTTTTGT GTACGAAGTC 
TGCAGCACCT CCCCGGATGG CTCTGAGTGT GTCACGCTTC CCGTGTACTT CAGGGAGAGG 
AAATCTAGGC CGTCGAGCAC TTCTACCGGG GCAGTGCTGT ACGGGCAGCC ACTGCTGGTT 
TCTGTCCCCA AGCACAAGCT CACCCTCGAG TCTTTGTACC AGGCGGTTTG TGAGCGCATC 
AGCCGCTATA TAAAACAGCC TTTGCCTGAT GAGTCAGGCA GCTCACCCTT GGAGCTGGGG 
GCCTGCAATG GCTCCAGGAG CGGCTGTGCA GGTGAAGATG AAGAAGAAAT GGAGCATCAG 
GAAGAAGGCA GAGAGCAGCT CTCAGAAACA GAGGGCAGTG GGGACGATGA GCCGGGAAGT 
GACCACAGCG AGGCCACCCA AAAGAAGAAC AAAGGCCGGC CTTGCCCCAG AAGGCTTTTT 
ACCTTCAGCC TTGTGAACTC CTATGGCACA GCTGACATAA ATTCCCTTGC CACTGATGGG 
AAACTACTGA AACTCAACTC TCGGTCCACA CTAGCCATCG ACTGGGACAG TGAGACCCGG 
AGCCGTTACT TCAACGAACA GGAATCTGAG ACGTACGAGA AGCACGTCAG CATGTTGCAA 
CCTCAGAAGA AAAAGAAGAC GGCGGTGGCC CTGAGGGACT GCATCGAGCT CTTCACTACC 
ATGGAGACCC TTGGGGAACA TGACCCCTGG TACTGTCCCA ACTGTAAGAA GCACCAGCAG 
GCCACAAAGA AGTTTGACCT GTGGTCCTTG CCGAAGATCC TGGTGGTCCA CCTCAAACGT 
TTCTCCTACA ATAGATACTG GAGGGATAAG CTTGATACAG TCGTGGAGTT CCCAGTCAGA 
GGTCTGAACA TGTCGGAGTT TGTCTGTGAC CCATCAGCGA GGCCGTATGT GTACGATCTC 
ATCGCTGTGT CCAATCACTA TGGAGCCATG GGGGTTGGCC ACTACACTGC ATACGCGAAG 
AACAAACTGA ACGGGAAGTG GTATTACTTT GATGATAGCA ATGTGTCCCT GGCCTGTGAG 
GATCAGATAG TGACGAAAGC AGCTTATGTG CTGTTTTACC AGCGTCGAGA CGATGAGTTT 
CATAAGACGC CGTCACTCAG TTTTCCTGGC TCTTCTGATG GAGGTGCAAG ACCAAGCAGC 
TCACAGCAGG GCACGGGGGA TGACGAGACT TACAGCATGG ACACCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006053313.2
CDS	69..2819
Translation	MAEGGGYRERPDAETQKSELGALMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLYPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPAEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTQQSNGSGFSASYNCQESPLTHVQPGLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRVMEIFLVPADPRCRPTQYRVVVPLMGAVSDLCEALSKLSGIAAENMVVTDVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSPDGSECVTLPVYFRERKSRPSSTSTGAVLYGQPLLVSVPKHKLTLESLYQAVCERISRYIKQPLPDESGSSPLELGACNGSRSGCAGEDEEEMEHQEEGREQLSETEGSGDDEPGSDHSEATQKKNKGRPCPRRLFTFSLVNSYGTADINSLATDGKLLKLNSRSTLAIDWDSETRSRYFNEQESETYEKHVSMLQPQKKKKTAVALRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTVVEFPVRGLNMSEFVCDPSARPYVYDLIAVSNHYGAMGVGHYTAYAKNKLNGKWYYFDDSNVSLACEDQIVTKAAYVLFYQRRDDEFHKTPSLSFPGSSDGGARPSSSQQGTGDDETYSMDTN

Target ORF information:

RefSeq Version	XM_006053251.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ubiquitin specific peptidase 4 (USP4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006053251.2

ORF Insert Sequence:

ATGGCGGAAG GCGGAGGGTA CCGTGAGCGA CCGGATGCGG AGACCCAGAA ATCCGAGCTC 
GGGGCCTTGA TGAGGACCAC GCTCCAGCGA GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTCGACAGCT GGGATATGTA CAATGTGGGT 
GAACATAATC TGTATCCTGG CCCGATAGAT AACTCCGGAC TCTTTTCAGA TCCTGAGAGT 
CAGACCTTAA AGGAACATTT AATTGATGAA TTGGACTATG TTTTGGTCCC AGCAGAGGCC 
TGGAATAAGT TACTAAACTG GTATGGCTGC GTAGAAGGCC AGCAGCCCAT TGTCAGAAAA 
GTGGTAGAAC ATGGCCTGTT TGTCAAGCAC TGCAAGGTGG AGGTATATTT GCTGGAACTG 
AAGCTTTGTG AGAACAGTGA CCCCACCAAC GTGCTGAGCT GCCATTTCAG CAAGGCAGAC 
ACCATCGCAA CCATCGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAG 
ACGCGGCTGT GGAACAAATA CATGAGCAAC ACCTACGAGC AGCTGAGCAA GCTGGACAAC 
ACTGTGCAGG ATGCCGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GATGGCACAT GGCCAAGGCA GACCCAGCAA TCCAATGGAT CTGGCTTCTC TGCTTCGTAT 
AATTGTCAGG AGTCCCCATT GACTCACGTC CAACCTGGGC TCTGCGGACT CGGAAACCTG 
GGGAACACCT GCTTCATGAA CTCCGCTTTG CAGTGCTTAA GCAACACAGC ACCGTTGACC 
GACTACTTTC TCAAAGATGA ATACGAAGCC GAAATCAACA GGGACAACCC CCTGGGAATG 
AAAGGGGAAA TTGCAGAGGC CTACGCAGAG CTCATTAAGC AGATGTGGTC TGGCAGGGAT 
GCTCATGTGG CACCTCGCAT GTTCAAAACT CAGGTGGGAC GTTTTGCCCC TCAGTTTTCT 
GGCTACCAAC AACAAGACTC TCAGGAGCTT TTAGCCTTTC TTCTAGATGG ATTGCATGAA 
GATTTGAACC GTGTGAAGAA AAAACCCTAC CTAGAGCTGA AGGATGCCAA TGGGCGGCCA 
GATGCGGTGG TGGCAAAGGA AGCCTGGGAG AATCATAGGT TGAGGAATGA TTCTGTGATC 
GTGGACACGT TCCATGGCCT CTTCAAATCT ACATTGGTTT GCCCAGAATG TGCTAAGGTT 
TCTGTGACCT TTGACCCATT TTGCTATCTA ACTCTCCCAC TGCCCTTGAA GAAAGACCGA 
GTTATGGAGA TCTTCCTAGT TCCTGCCGAC CCTCGCTGCA GGCCTACCCA GTACCGTGTG 
GTTGTGCCAC TGATGGGGGC CGTGTCTGAC CTGTGTGAGG CTCTCTCCAA ACTGTCTGGC 
ATTGCTGCAG AAAACATGGT GGTCACAGAT GTGTATAATC ACCGGTTCCA CAAGATTTTC 
CAAATGGATG AAGGTTTAAA CCACATCATG CCTCGGGATG ACATTTTTGT GTACGAAGTC 
TGCAGCACCT CCCCGGATGG CTCTGAGTGT GTCACGCTTC CCGTGTACTT CAGGGAGAGG 
AAATCTAGGC CGTCGAGCAC TTCTACCGGG GCAGTGCTGT ACGGGCAGCC ACTGCTGGTT 
TCTGTCCCCA AGCACAAGCT CACCCTCGAG TCTTTGTACC AGGCGGTTTG TGAGCGCATC 
AGCCGCTATA TAAAACAGCC TTTGCCTGAT GAGTCAGGCA GCTCACCCTT GGAGCTGGGG 
GCCTGCAATG GCTCCAGGAG CGGCTGTGCA GGTGAAGATG AAGAAGAAAT GGAGCATCAG 
GAAGAAGGCA GAGAGCAGCT CTCAGAAACA GAGGGCAGTG GGGACGATGA GCCGGGAAGT 
GACCACAGCG AGGCCACCCA AAAGAAGAAC AAAGGCCGGC CTTGCCCCAG AAGGCTTTTT 
ACCTTCAGCC TTGTGAACTC CTATGGCACA GCTGACATAA ATTCCCTTGC CACTGATGGG 
AAACTACTGA AACTCAACTC TCGGTCCACA CTAGCCATCG ACTGGGACAG TGAGACCCGG 
AGCCGTTACT TCAACGAACA GGAATCTGAG ACGTACGAGA AGCACGTCAG CATGTTGCAA 
CCTCAGAAGA AAAAGAAGAC GGCGGTGGCC CTGAGGGACT GCATCGAGCT CTTCACTACC 
ATGGAGACCC TTGGGGAACA TGACCCCTGG TACTGTCCCA ACTGTAAGAA GCACCAGCAG 
GCCACAAAGA AGTTTGACCT GTGGTCCTTG CCGAAGATCC TGGTGGTCCA CCTCAAACGT 
TTCTCCTACA ATAGATACTG GAGGGATAAG CTTGATACAG TCGTGGAGTT CCCAGTCAGA 
GGTCTGAACA TGTCGGAGTT TGTCTGTGAC CCATCAGCGA GGCCGTATGT GTACGATCTC 
ATCGCTGTGT CCAATCACTA TGGAGCCATG GGGGTTGGCC ACTACACTGC ATACGCGAAG 
AACAAACTGA ACGGGAAGTG GTATTACTTT GATGATAGCA ATGTGTCCCT GGCCTGTGAG 
GATCAGATAG TGACGAAAGC AGCTTATGTG CTGTTTTACC AGCGTCGAGA CGATGAGTTT 
CATAAGACGC CGTCACTCAG TTTTCCTGGC TCTTCTGATG GAGGTGCAAG ACCAAGCAGC 
TCACAGCAGG GCACGGGGGA TGACGAGACT TACAGCATGG ACACCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP4 ( XM_025272022.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025127807.1 Bubalus bubalis ubiquitin specific peptidase 4 (USP4), transcript variant X4, mRNA.

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CloneID	OBu57441
Clone ID Related Accession (Same CDS sequence)	XM_025272022.1
Accession Version	XM_025272022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ubiquitin carboxyl-terminal hydrolase 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG GCGGAGGGTA CCGTGAGCGA CCGGATGCGG AGACCCAGAA ATCCGAGCTC 
GGGGCCTTGA TGAGGACCAC GCTCCAGCGA GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTCGACAGCT GGGATATGTA CAATGTGGGT 
GAACATAATC TGTATCCTGG CCCGATAGAT AACTCCGGAC TCTTTTCAGA TCCTGAGAGT 
CAGACCTTAA AGGAACATTT AATTGATGAA TTGGACTATG TTTTGGTCCC AGCAGAGGCC 
TGGAATAAGT TACTAAACTG GTATGGCTGC GTAGAAGGCC AGCAGCCCAT TGTCAGAAAA 
GTGGTAGAAC ATGGCCTGTT TGTCAAGCAC TGCAAGGTGG AGGTATATTT GCTGGAACTG 
AAGCTTTGTG AGAACAGTGA CCCCACCAAC GTGCTGAGCT GCCATTTCAG CAAGGCAGAC 
ACCATCGCAA CCATCGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAG 
ACGCGGCTGT GGAACAAATA CATGAGCAAC ACCTACGAGC AGCTGAGCAA GCTGGACAAC 
ACTGTGCAGG ATGCCGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GATGGCACAT GGCCAAGGCA GACCCAGCAA TCCAAATCAA GCACTGCGCC TAGCAGAAAT 
TTTACTACCT CTCCAAAATC ATCAGCAAGT CCCTATTCCT CAGTGTCTGC CTCTCCCATT 
GCAAATGGTG ATAGCACTAA CACCTCGGGG ATGCACAGCT CCGGTGTCAG CAGGGGTGGA 
TCTGGCTTCT CTGCTTCGTA TAATTGTCAG GAGTCCCCAT TGACTCACGT CCAACCTGGG 
CTCTGCGGAC TCGGAAACCT GGGGAACACC TGCTTCATGA ACTCCGCTTT GCAGTGCTTA 
AGCAACACAG CACCGTTGAC CGACTACTTT CTCAAAGATG AATACGAAGC CGAAATCAAC 
AGGGACAACC CCCTGGGAAT GAAAGGGGAA ATTGCAGAGG CCTACGCAGA GCTCATTAAG 
CAGATGTGGT CTGGCAGGGA TGCTCATGTG GCACCTCGCA TGTTCAAAAC TCAGGTGGGA 
CGTTTTGCCC CTCAGTTTTC TGGCTACCAA CAACAAGACT CTCAGGAGCT TTTAGCCTTT 
CTTCTAGATG GATTGCATGA AGATTTGAAC CGTGTGAAGA AAAAACCCTA CCTAGAGCTG 
AAGGATGCCA ATGGGCGGCC AGATGCGGTG GTGGCAAAGG AAGCCTGGGA GAATCATAGG 
TTGAGGAATG ATTCTGTGAT CGTGGACACG TTCCATGGCC TCTTCAAATC TACATTGGTT 
TGCCCAGAAT GTGCTAAGGT TTCTGTGACC TTTGACCCAT TTTGCTATCT AACTCTCCCA 
CTGCCCTTGA AGAAAGACCG AGTTATGGAG ATCTTCCTAG TTCCTGCCGA CCCTCGCTGC 
AGGCCTACCC AGTACCGTGT GGTTGTGCCA CTGATGGGGG CCGTGTCTGA CCTGTGTGAG 
GCTCTCTCCA AACTGTCTGG CATTGCTGCA GAAAACATGG TGGTCACAGA TGTGTATAAT 
CACCGGTTCC ACAAGATTTT CCAAATGGAT GAAGGTTTAA ACCACATCAT GCCTCGGGAT 
GACATTTTTG TGTACGAAGT CTGCAGCACC TCCCCGGATG GCTCTGAGTG TGTCACGCTT 
CCCGTGTACT TCAGGGAGAG GAAATCTAGG CCGTCGAGCA CTTCTACCGG GGCAGTGCTG 
TACGGGCAGC CACTGCTGGT TTCTGTCCCC AAGCACAAGC TCACCCTCGA GTCTTTGTAC 
CAGGCGGTTT GTGAGCGCAT CAGCCGCTAT ATAAAACAGC CTTTGCCTGA TGAGTCAGGC 
AGCTCACCCT TGGAGCTGGG GGCCTGCAAT GGCTCCAGGA GCGGCTGTGC AGGTGAAGAT 
GAAGAAGAAA TGGAGCATCA GGAAGAAGGC AGAGAGCAGC TCTCAGAAAC AGAGGGCAGT 
GGGGACGATG AGCCGGGAAG TGACCACAGC GAGGCCACCC AAAAGAAGAA CAAAGGCCGG 
CCTTGCCCCA GAAGGCTTTT TACCTTCAGC CTTGTGAACT CCTATGGCAC AGCTGACATA 
AATTCCCTTG CCACTGATGG GAAACTACTG AAACTCAACT CTCGGTCCAC ACTAGCCATC 
GACTGGGACA GTGAGACCCG GAGCCGTTAC TTCAACGAAC AGGAATCTGA GACGTACGAG 
AAGCACGTCA GCATGTTGCA ACCTCAGAAG AAAAAGAAGA CGGCGGTGGC CCTGAGGGAC 
TGCATCGAGC TCTTCACTAC CATGGAGACC CTTGGGGAAC ATGACCCCTG GTACTGTCCC 
AACTGTAAGA AGCACCAGCA GGCCACAAAG AAGTTTGACC TGTGGTCCTT GCCGAAGATC 
CTGGTGGTCC ACCTCAAACG TTTCTCCTAC AATAGATACT GGAGGGATAA GCTTGATACA 
GTCGTGGAGT TCCCAGTCAG ACACTGCATA CGCGAAGAAC AAACTGAACG GGAAGTGGTA 
TTACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025127807.1
CDS	70..2658
Translation	MAEGGGYRERPDAETQKSELGALMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLYPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPAEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTQQSKSSTAPSRNFTTSPKSSASPYSSVSASPIANGDSTNTSGMHSSGVSRGGSGFSASYNCQESPLTHVQPGLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRVMEIFLVPADPRCRPTQYRVVVPLMGAVSDLCEALSKLSGIAAENMVVTDVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSPDGSECVTLPVYFRERKSRPSSTSTGAVLYGQPLLVSVPKHKLTLESLYQAVCERISRYIKQPLPDESGSSPLELGACNGSRSGCAGEDEEEMEHQEEGREQLSETEGSGDDEPGSDHSEATQKKNKGRPCPRRLFTFSLVNSYGTADINSLATDGKLLKLNSRSTLAIDWDSETRSRYFNEQESETYEKHVSMLQPQKKKKTAVALRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTVVEFPVRHCIREEQTEREVVLL

Target ORF information:

RefSeq Version	XM_025272022.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ubiquitin specific peptidase 4 (USP4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025272022.1

ORF Insert Sequence:

ATGGCGGAAG GCGGAGGGTA CCGTGAGCGA CCGGATGCGG AGACCCAGAA ATCCGAGCTC 
GGGGCCTTGA TGAGGACCAC GCTCCAGCGA GGGGCGCAGT GGTATCTTAT TGACAGCCGG 
TGGTTCAAGC AGTGGAAGAA GTATGTGGGC TTCGACAGCT GGGATATGTA CAATGTGGGT 
GAACATAATC TGTATCCTGG CCCGATAGAT AACTCCGGAC TCTTTTCAGA TCCTGAGAGT 
CAGACCTTAA AGGAACATTT AATTGATGAA TTGGACTATG TTTTGGTCCC AGCAGAGGCC 
TGGAATAAGT TACTAAACTG GTATGGCTGC GTAGAAGGCC AGCAGCCCAT TGTCAGAAAA 
GTGGTAGAAC ATGGCCTGTT TGTCAAGCAC TGCAAGGTGG AGGTATATTT GCTGGAACTG 
AAGCTTTGTG AGAACAGTGA CCCCACCAAC GTGCTGAGCT GCCATTTCAG CAAGGCAGAC 
ACCATCGCAA CCATCGAGAA GGAGATGAGG AAGCTCTTCA ACATCCCTGC AGAGCGTGAG 
ACGCGGCTGT GGAACAAATA CATGAGCAAC ACCTACGAGC AGCTGAGCAA GCTGGACAAC 
ACTGTGCAGG ATGCCGGGCT GTACCAGGGT CAGGTGCTAG TAATTGAGCC CCAAAATGAA 
GATGGCACAT GGCCAAGGCA GACCCAGCAA TCCAAATCAA GCACTGCGCC TAGCAGAAAT 
TTTACTACCT CTCCAAAATC ATCAGCAAGT CCCTATTCCT CAGTGTCTGC CTCTCCCATT 
GCAAATGGTG ATAGCACTAA CACCTCGGGG ATGCACAGCT CCGGTGTCAG CAGGGGTGGA 
TCTGGCTTCT CTGCTTCGTA TAATTGTCAG GAGTCCCCAT TGACTCACGT CCAACCTGGG 
CTCTGCGGAC TCGGAAACCT GGGGAACACC TGCTTCATGA ACTCCGCTTT GCAGTGCTTA 
AGCAACACAG CACCGTTGAC CGACTACTTT CTCAAAGATG AATACGAAGC CGAAATCAAC 
AGGGACAACC CCCTGGGAAT GAAAGGGGAA ATTGCAGAGG CCTACGCAGA GCTCATTAAG 
CAGATGTGGT CTGGCAGGGA TGCTCATGTG GCACCTCGCA TGTTCAAAAC TCAGGTGGGA 
CGTTTTGCCC CTCAGTTTTC TGGCTACCAA CAACAAGACT CTCAGGAGCT TTTAGCCTTT 
CTTCTAGATG GATTGCATGA AGATTTGAAC CGTGTGAAGA AAAAACCCTA CCTAGAGCTG 
AAGGATGCCA ATGGGCGGCC AGATGCGGTG GTGGCAAAGG AAGCCTGGGA GAATCATAGG 
TTGAGGAATG ATTCTGTGAT CGTGGACACG TTCCATGGCC TCTTCAAATC TACATTGGTT 
TGCCCAGAAT GTGCTAAGGT TTCTGTGACC TTTGACCCAT TTTGCTATCT AACTCTCCCA 
CTGCCCTTGA AGAAAGACCG AGTTATGGAG ATCTTCCTAG TTCCTGCCGA CCCTCGCTGC 
AGGCCTACCC AGTACCGTGT GGTTGTGCCA CTGATGGGGG CCGTGTCTGA CCTGTGTGAG 
GCTCTCTCCA AACTGTCTGG CATTGCTGCA GAAAACATGG TGGTCACAGA TGTGTATAAT 
CACCGGTTCC ACAAGATTTT CCAAATGGAT GAAGGTTTAA ACCACATCAT GCCTCGGGAT 
GACATTTTTG TGTACGAAGT CTGCAGCACC TCCCCGGATG GCTCTGAGTG TGTCACGCTT 
CCCGTGTACT TCAGGGAGAG GAAATCTAGG CCGTCGAGCA CTTCTACCGG GGCAGTGCTG 
TACGGGCAGC CACTGCTGGT TTCTGTCCCC AAGCACAAGC TCACCCTCGA GTCTTTGTAC 
CAGGCGGTTT GTGAGCGCAT CAGCCGCTAT ATAAAACAGC CTTTGCCTGA TGAGTCAGGC 
AGCTCACCCT TGGAGCTGGG GGCCTGCAAT GGCTCCAGGA GCGGCTGTGC AGGTGAAGAT 
GAAGAAGAAA TGGAGCATCA GGAAGAAGGC AGAGAGCAGC TCTCAGAAAC AGAGGGCAGT 
GGGGACGATG AGCCGGGAAG TGACCACAGC GAGGCCACCC AAAAGAAGAA CAAAGGCCGG 
CCTTGCCCCA GAAGGCTTTT TACCTTCAGC CTTGTGAACT CCTATGGCAC AGCTGACATA 
AATTCCCTTG CCACTGATGG GAAACTACTG AAACTCAACT CTCGGTCCAC ACTAGCCATC 
GACTGGGACA GTGAGACCCG GAGCCGTTAC TTCAACGAAC AGGAATCTGA GACGTACGAG 
AAGCACGTCA GCATGTTGCA ACCTCAGAAG AAAAAGAAGA CGGCGGTGGC CCTGAGGGAC 
TGCATCGAGC TCTTCACTAC CATGGAGACC CTTGGGGAAC ATGACCCCTG GTACTGTCCC 
AACTGTAAGA AGCACCAGCA GGCCACAAAG AAGTTTGACC TGTGGTCCTT GCCGAAGATC 
CTGGTGGTCC ACCTCAAACG TTTCTCCTAC AATAGATACT GGAGGGATAA GCTTGATACA 
GTCGTGGAGT TCCCAGTCAG ACACTGCATA CGCGAAGAAC AAACTGAACG GGAAGTGGTA 
TTACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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USP4 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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