PHLDB2 gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006046550.1	XP_006046612.1	pleckstrin homology-like domain family B member 2 isoform X3
XM_025283820.1	XP_025139605.1	pleckstrin homology-like domain family B member 2 isoform X2
XM_025283825.1	XP_025139610.1	pleckstrin homology-like domain family B member 2 isoform X3
XM_025283908.1	XP_025139693.1	pleckstrin homology-like domain family B member 2 isoform X13
XM_025283918.1	XP_025139703.1	pleckstrin homology-like domain family B member 2 isoform X16
XM_025283834.1	XP_025139619.1	pleckstrin homology-like domain family B member 2 isoform X4
XM_025283875.1	XP_025139660.1	pleckstrin homology-like domain family B member 2 isoform X7
XM_025283883.1	XP_025139668.1	pleckstrin homology-like domain family B member 2 isoform X8
XM_025283891.1	XP_025139676.1	pleckstrin homology-like domain family B member 2 isoform X9
XM_025283914.1	XP_025139699.1	pleckstrin homology-like domain family B member 2 isoform X15
XM_006046548.1	XP_006046610.1	pleckstrin homology-like domain family B member 2 isoform X1
XM_006046551.1	XP_006046613.1	pleckstrin homology-like domain family B member 2 isoform X4
XM_025283861.1	XP_025139646.1	pleckstrin homology-like domain family B member 2 isoform X5
XM_025283911.1	XP_025139696.1	pleckstrin homology-like domain family B member 2 isoform X14
XM_006046549.1	XP_006046611.1	pleckstrin homology-like domain family B member 2 isoform X2
XM_025283816.1	XP_025139601.1	pleckstrin homology-like domain family B member 2 isoform X1
XM_025283874.1	XP_025139659.1	pleckstrin homology-like domain family B member 2 isoform X6
XM_025283839.1	XP_025139624.1	pleckstrin homology-like domain family B member 2 isoform X5
XM_025283846.1	XP_025139631.1	pleckstrin homology-like domain family B member 2 isoform X5
XM_025283855.1	XP_025139640.1	pleckstrin homology-like domain family B member 2 isoform X5
XM_025283866.1	XP_025139651.1	pleckstrin homology-like domain family B member 2 isoform X5
XM_025283868.1	XP_025139653.1	pleckstrin homology-like domain family B member 2 isoform X5
XM_025283897.1	XP_025139682.1	pleckstrin homology-like domain family B member 2 isoform X10
XM_025283901.1	XP_025139686.1	pleckstrin homology-like domain family B member 2 isoform X11
XM_025283905.1	XP_025139690.1	pleckstrin homology-like domain family B member 2 isoform X12

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu05331	XM_006046548.1 Latest version!	Bubalus bubalis pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBu05332	XM_006046549.1 Latest version!	Bubalus bubalis pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBu05333	XM_006046550.1 Latest version!	Bubalus bubalis pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBu05334	XM_006046551.1 Latest version!	Bubalus bubalis pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBu42732	XM_025283816.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu42733	XM_025283820.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu42734	XM_025283825.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu42735	XM_025283834.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu42736	XM_025283846.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu42736	XM_025283855.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu42736	XM_025283861.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu42736	XM_025283866.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu42736	XM_025283868.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu42736	XM_025283839.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu42737	XM_025283874.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu42738	XM_025283875.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu42739	XM_025283883.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu42740	XM_025283891.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu42741	XM_025283897.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu42742	XM_025283901.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu42743	XM_025283905.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu42744	XM_025283908.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu42745	XM_025283911.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu42746	XM_025283914.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu42747	XM_025283918.1 Latest version!	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

PHLDB2 ( XM_006046548.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006046610.1 Bubalus bubalis pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu05331
Clone ID Related Accession (Same CDS sequence)	XM_006046548.1
Accession Version	XM_006046548.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783892.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAGC CACAAAGTAA AGAGCAAAGA TCGTCTGTCT GTCCTGGATT TATGTTTCCT 
TCCACTCTTT CTTCTCATCC TATCACTCCA CCTCCATCAG CCCCTTGGCC TGAAGCTGTG 
GCTACCTCTG TCGATTCTTT TCCTTTAAAT GACACACCTC CTCCTCTACC AGCTAAGAAA 
CACAGGAGGC AACAGCAGCA GGACCAGCAG CATTTTAGAA GTCTGGAAGA AAGGAAAAAA 
CAGCACCAGG AAGGCCTCTA TCTGAGTGAC ACTTTGCCTC GAAAGAAAAC CACACCTTCC 
ATATCCCCAC ATTTCAGCAG TGCTACTATG GGGAGAAGCA CCACCCCAAA GGGCCACCTG 
CCCCTGGGAC AGAGCAACAG CTGTGGCAGC GTGCTCCCCC ACTCGCTAGC AACCATGACC 
AAAGACTCAG AATCTCGGAG GATGCTCAGA GGGAAGTCCA AGAAAGGAAG AATGAATTTC 
TCAGCAGGAG CAGTTAGTAA TTCCAAAAGT TCAAGTGTCA AAGGATATAA TCACCAACAG 
ATGAGTGAAG GACAAAGGCA GAAGTCTTCT GAATTTTGCA ACCGCACAGC ATCTGAATCG 
AATGTCTACT TGAACAGTTT CCACTACCCA GATCACAGCT ACAAGGACCA GGCCTTTGAT 
ACTCTGAGCC TAGACAGCTC TGACAGCATG GAGACCAGCA TCTCTGCCTG CTCACCTGAC 
AATATCTCTA GTGCCAGCAC TTCAAATATT GCCCGAATAG AAGAAATGGA GAGACTGTTG 
AAGCAGGCTC ATGCAGAGAA GACCAGGCTG CTTGAATCCA GGGAACGAGA AATGGAAGCC 
AAGAAACGCG CTCTGGAAGA AGAAAAACGA CGTCGAGAAC TCCTGGAAAA ACGGTTGCAG 
GAAGAAACTA GCCAGAGGCA GAAGTTAATT GAAAAGGAAG TAAAAATAAG GGAGAAACAA 
AGGGCACAGG CTCGACCTCT GACCCGCTAT CTGCCTGTGC GGAAGGAAGA CTTTGATTTG 
CGGAGCCACG TAGAGACTGC TGGCCACAAT ATCGATACGT GTTATCATGT GTCAATCACA 
GAGAAGACCT GCAGAGGATT CCTCATCAAA ATGGGTGGAA AAATTAAAAC CTGGAAAAAA 
CGTTGGTTTG TTTTTGATCG GAACAAGCGC ACCTTCTCTT ATTATGCAGA CAAGCATGAA 
GCTAAATTAA AAGGAGTAAT ATACTTTCAA GCCATTGAAG AAGTATATTA TGATCACCTC 
AAGAATGCTA ATAAGAGTCC TAATCCTTTA CTTACCTTTA GCGTCAAGAC ACATGACAGA 
ATCTACTACA TGGTGGCCCC GTCACCAGAA GCCATGCGGA TCTGGATGGA CGTTATAGTG 
ACTGGGGCAG AAGGCTACAC TCACTTCTTG TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006046610.1
CDS	1085..2500
Translation	MSQPQSKEQRSSVCPGFMFPSTLSSHPITPPPSAPWPEAVATSVDSFPLNDTPPPLPAKKHRRQQQQDQQHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGKSKKGRMNFSAGAVSNSKSSSVKGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_006046548.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006046548.1

ORF Insert Sequence:

ATGAGCCAGC CACAAAGTAA AGAGCAAAGA TCGTCTGTCT GTCCTGGATT TATGTTTCCT 
TCCACTCTTT CTTCTCATCC TATCACTCCA CCTCCATCAG CCCCTTGGCC TGAAGCTGTG 
GCTACCTCTG TCGATTCTTT TCCTTTAAAT GACACACCTC CTCCTCTACC AGCTAAGAAA 
CACAGGAGGC AACAGCAGCA GGACCAGCAG CATTTTAGAA GTCTGGAAGA AAGGAAAAAA 
CAGCACCAGG AAGGCCTCTA TCTGAGTGAC ACTTTGCCTC GAAAGAAAAC CACACCTTCC 
ATATCCCCAC ATTTCAGCAG TGCTACTATG GGGAGAAGCA CCACCCCAAA GGGCCACCTG 
CCCCTGGGAC AGAGCAACAG CTGTGGCAGC GTGCTCCCCC ACTCGCTAGC AACCATGACC 
AAAGACTCAG AATCTCGGAG GATGCTCAGA GGGAAGTCCA AGAAAGGAAG AATGAATTTC 
TCAGCAGGAG CAGTTAGTAA TTCCAAAAGT TCAAGTGTCA AAGGATATAA TCACCAACAG 
ATGAGTGAAG GACAAAGGCA GAAGTCTTCT GAATTTTGCA ACCGCACAGC ATCTGAATCG 
AATGTCTACT TGAACAGTTT CCACTACCCA GATCACAGCT ACAAGGACCA GGCCTTTGAT 
ACTCTGAGCC TAGACAGCTC TGACAGCATG GAGACCAGCA TCTCTGCCTG CTCACCTGAC 
AATATCTCTA GTGCCAGCAC TTCAAATATT GCCCGAATAG AAGAAATGGA GAGACTGTTG 
AAGCAGGCTC ATGCAGAGAA GACCAGGCTG CTTGAATCCA GGGAACGAGA AATGGAAGCC 
AAGAAACGCG CTCTGGAAGA AGAAAAACGA CGTCGAGAAC TCCTGGAAAA ACGGTTGCAG 
GAAGAAACTA GCCAGAGGCA GAAGTTAATT GAAAAGGAAG TAAAAATAAG GGAGAAACAA 
AGGGCACAGG CTCGACCTCT GACCCGCTAT CTGCCTGTGC GGAAGGAAGA CTTTGATTTG 
CGGAGCCACG TAGAGACTGC TGGCCACAAT ATCGATACGT GTTATCATGT GTCAATCACA 
GAGAAGACCT GCAGAGGATT CCTCATCAAA ATGGGTGGAA AAATTAAAAC CTGGAAAAAA 
CGTTGGTTTG TTTTTGATCG GAACAAGCGC ACCTTCTCTT ATTATGCAGA CAAGCATGAA 
GCTAAATTAA AAGGAGTAAT ATACTTTCAA GCCATTGAAG AAGTATATTA TGATCACCTC 
AAGAATGCTA ATAAGAGTCC TAATCCTTTA CTTACCTTTA GCGTCAAGAC ACATGACAGA 
ATCTACTACA TGGTGGCCCC GTCACCAGAA GCCATGCGGA TCTGGATGGA CGTTATAGTG 
ACTGGGGCAG AAGGCTACAC TCACTTCTTG TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_006046549.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006046611.1 Bubalus bubalis pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu05332
Clone ID Related Accession (Same CDS sequence)	XM_006046549.1
Accession Version	XM_006046549.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783892.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAGC CACAAAGTAA AGAGCAAAGA TCGTCTGTCT GTCCTGGATT TATGTTTCCT 
TCCACTCTTT CTTCTCATCC TATCACTCCA CCTCCATCAG CCCCTTGGCC TGAAGCTGTG 
GCTACCTCTG TCGATTCTTT TCCTTTAAAT GACACACCTC CTCCTCTACC AGCTAAGAAA 
CACAGGAGGC AACAGCAGCA GGACCAGCAG CATTTTAGAA GTCTGGAAGA AAGGAAAAAA 
CAGCACCAGG AAGGCCTCTA TCTGAGTGAC ACTTTGCCTC GAAAGAAAAC CACACCTTCC 
ATATCCCCAC ATTTCAGCAG TGCTACTATG GGGAGAAGCA CCACCCCAAA GGGCCACCTG 
CCCCTGGGAC AGAGCAACAG CTGTGGCAGC GTGCTCCCCC ACTCGCTAGC AACCATGACC 
AAAGACTCAG AATCTCGGAG GATGCTCAGA GGGAAGTCCA AGAAAGGAAG AATGAATTTC 
TCAGCAGGAT ATAATCACCA ACAGATGAGT GAAGGACAAA GGCAGAAGTC TTCTGAATTT 
TGCAACCGCA CAGCATCTGA ATCGAATGTC TACTTGAACA GTTTCCACTA CCCAGATCAC 
AGCTACAAGG ACCAGGCCTT TGATACTCTG AGCCTAGACA GCTCTGACAG CATGGAGACC 
AGCATCTCTG CCTGCTCACC TGACAATATC TCTAGTGCCA GCACTTCAAA TATTGCCCGA 
ATAGAAGAAA TGGAGAGACT GTTGAAGCAG GCTCATGCAG AGAAGACCAG GCTGCTTGAA 
TCCAGGGAAC GAGAAATGGA AGCCAAGAAA CGCGCTCTGG AAGAAGAAAA ACGACGTCGA 
GAACTCCTGG AAAAACGGTT GCAGGAAGAA ACTAGCCAGA GGCAGAAGTT AATTGAAAAG 
GAAGTAAAAA TAAGGGAGAA ACAAAGGGCA CAGGCTCGAC CTCTGACCCG CTATCTGCCT 
GTGCGGAAGG AAGACTTTGA TTTGCGGAGC CACGTAGAGA CTGCTGGCCA CAATATCGAT 
ACGTGTTATC ATGTGTCAAT CACAGAGAAG ACCTGCAGAG GATTCCTCAT CAAAATGGGT 
GGAAAAATTA AAACCTGGAA AAAACGTTGG TTTGTTTTTG ATCGGAACAA GCGCACCTTC 
TCTTATTATG CAGACAAGCA TGAAGCTAAA TTAAAAGGAG TAATATACTT TCAAGCCATT 
GAAGAAGTAT ATTATGATCA CCTCAAGAAT GCTAATAAGA GTCCTAATCC TTTACTTACC 
TTTAGCGTCA AGACACATGA CAGAATCTAC TACATGGTGG CCCCGTCACC AGAAGCCATG 
CGGATCTGGA TGGACGTTAT AGTGACTGGG GCAGAAGGCT ACACTCACTT CTTGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006046611.1
CDS	1084..2463
Translation	MSQPQSKEQRSSVCPGFMFPSTLSSHPITPPPSAPWPEAVATSVDSFPLNDTPPPLPAKKHRRQQQQDQQHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGKSKKGRMNFSAGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_006046549.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006046549.1

ORF Insert Sequence:

ATGAGCCAGC CACAAAGTAA AGAGCAAAGA TCGTCTGTCT GTCCTGGATT TATGTTTCCT 
TCCACTCTTT CTTCTCATCC TATCACTCCA CCTCCATCAG CCCCTTGGCC TGAAGCTGTG 
GCTACCTCTG TCGATTCTTT TCCTTTAAAT GACACACCTC CTCCTCTACC AGCTAAGAAA 
CACAGGAGGC AACAGCAGCA GGACCAGCAG CATTTTAGAA GTCTGGAAGA AAGGAAAAAA 
CAGCACCAGG AAGGCCTCTA TCTGAGTGAC ACTTTGCCTC GAAAGAAAAC CACACCTTCC 
ATATCCCCAC ATTTCAGCAG TGCTACTATG GGGAGAAGCA CCACCCCAAA GGGCCACCTG 
CCCCTGGGAC AGAGCAACAG CTGTGGCAGC GTGCTCCCCC ACTCGCTAGC AACCATGACC 
AAAGACTCAG AATCTCGGAG GATGCTCAGA GGGAAGTCCA AGAAAGGAAG AATGAATTTC 
TCAGCAGGAT ATAATCACCA ACAGATGAGT GAAGGACAAA GGCAGAAGTC TTCTGAATTT 
TGCAACCGCA CAGCATCTGA ATCGAATGTC TACTTGAACA GTTTCCACTA CCCAGATCAC 
AGCTACAAGG ACCAGGCCTT TGATACTCTG AGCCTAGACA GCTCTGACAG CATGGAGACC 
AGCATCTCTG CCTGCTCACC TGACAATATC TCTAGTGCCA GCACTTCAAA TATTGCCCGA 
ATAGAAGAAA TGGAGAGACT GTTGAAGCAG GCTCATGCAG AGAAGACCAG GCTGCTTGAA 
TCCAGGGAAC GAGAAATGGA AGCCAAGAAA CGCGCTCTGG AAGAAGAAAA ACGACGTCGA 
GAACTCCTGG AAAAACGGTT GCAGGAAGAA ACTAGCCAGA GGCAGAAGTT AATTGAAAAG 
GAAGTAAAAA TAAGGGAGAA ACAAAGGGCA CAGGCTCGAC CTCTGACCCG CTATCTGCCT 
GTGCGGAAGG AAGACTTTGA TTTGCGGAGC CACGTAGAGA CTGCTGGCCA CAATATCGAT 
ACGTGTTATC ATGTGTCAAT CACAGAGAAG ACCTGCAGAG GATTCCTCAT CAAAATGGGT 
GGAAAAATTA AAACCTGGAA AAAACGTTGG TTTGTTTTTG ATCGGAACAA GCGCACCTTC 
TCTTATTATG CAGACAAGCA TGAAGCTAAA TTAAAAGGAG TAATATACTT TCAAGCCATT 
GAAGAAGTAT ATTATGATCA CCTCAAGAAT GCTAATAAGA GTCCTAATCC TTTACTTACC 
TTTAGCGTCA AGACACATGA CAGAATCTAC TACATGGTGG CCCCGTCACC AGAAGCCATG 
CGGATCTGGA TGGACGTTAT AGTGACTGGG GCAGAAGGCT ACACTCACTT CTTGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_006046550.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006046612.1 Bubalus bubalis pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu05333
Clone ID Related Accession (Same CDS sequence)	XM_006046550.1
Accession Version	XM_006046550.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783892.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAGC CACAAAGTAA AGAGCAAAGA TCGTCTGTCT GTCCTGGATT TATGTTTCCT 
TCCACTCTTT CTTCTCATCC TATCACTCCA CCTCCATCAG CCCCTTGGCC TGAAGCTGTG 
GCTACCTCTG TCGATTCTTT TCCTTTAAAT GACACACCTC CTCCTCTACC AGCTAAGAAA 
CACAGGAGGC AACAGCAGCA GGACCAGCAG CATTTTAGAA GTCTGGAAGA AAGGAAAAAA 
CAGCACCAGG AAGGCCTCTA TCTGAGTGAC ACTTTGCCTC GAAAGAAAAC CACACCTTCC 
ATATCCCCAC ATTTCAGCAG TGCTACTATG GGGAGAAGCA CCACCCCAAA GGGCCACCTG 
CCCCTGGGAC AGAGCAACAG CTGTGGCAGC GTGCTCCCCC ACTCGCTAGC AACCATGACC 
AAAGACTCAG AATCTCGGAG GATGCTCAGA GGATATAATC ACCAACAGAT GAGTGAAGGA 
CAAAGGCAGA AGTCTTCTGA ATTTTGCAAC CGCACAGCAT CTGAATCGAA TGTCTACTTG 
AACAGTTTCC ACTACCCAGA TCACAGCTAC AAGGACCAGG CCTTTGATAC TCTGAGCCTA 
GACAGCTCTG ACAGCATGGA GACCAGCATC TCTGCCTGCT CACCTGACAA TATCTCTAGT 
GCCAGCACTT CAAATATTGC CCGAATAGAA GAAATGGAGA GACTGTTGAA GCAGGCTCAT 
GCAGAGAAGA CCAGGCTGCT TGAATCCAGG GAACGAGAAA TGGAAGCCAA GAAACGCGCT 
CTGGAAGAAG AAAAACGACG TCGAGAACTC CTGGAAAAAC GGTTGCAGGA AGAAACTAGC 
CAGAGGCAGA AGTTAATTGA AAAGGAAGTA AAAATAAGGG AGAAACAAAG GGCACAGGCT 
CGACCTCTGA CCCGCTATCT GCCTGTGCGG AAGGAAGACT TTGATTTGCG GAGCCACGTA 
GAGACTGCTG GCCACAATAT CGATACGTGT TATCATGTGT CAATCACAGA GAAGACCTGC 
AGAGGATTCC TCATCAAAAT GGGTGGAAAA ATTAAAACCT GGAAAAAACG TTGGTTTGTT 
TTTGATCGGA ACAAGCGCAC CTTCTCTTAT TATGCAGACA AGCATGAAGC TAAATTAAAA 
GGAGTAATAT ACTTTCAAGC CATTGAAGAA GTATATTATG ATCACCTCAA GAATGCTAAT 
AAGAGTCCTA ATCCTTTACT TACCTTTAGC GTCAAGACAC ATGACAGAAT CTACTACATG 
GTGGCCCCGT CACCAGAAGC CATGCGGATC TGGATGGACG TTATAGTGAC TGGGGCAGAA 
GGCTACACTC ACTTCTTGTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006046612.1
CDS	1082..2425
Translation	MSQPQSKEQRSSVCPGFMFPSTLSSHPITPPPSAPWPEAVATSVDSFPLNDTPPPLPAKKHRRQQQQDQQHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_006046550.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006046550.1

ORF Insert Sequence:

ATGAGCCAGC CACAAAGTAA AGAGCAAAGA TCGTCTGTCT GTCCTGGATT TATGTTTCCT 
TCCACTCTTT CTTCTCATCC TATCACTCCA CCTCCATCAG CCCCTTGGCC TGAAGCTGTG 
GCTACCTCTG TCGATTCTTT TCCTTTAAAT GACACACCTC CTCCTCTACC AGCTAAGAAA 
CACAGGAGGC AACAGCAGCA GGACCAGCAG CATTTTAGAA GTCTGGAAGA AAGGAAAAAA 
CAGCACCAGG AAGGCCTCTA TCTGAGTGAC ACTTTGCCTC GAAAGAAAAC CACACCTTCC 
ATATCCCCAC ATTTCAGCAG TGCTACTATG GGGAGAAGCA CCACCCCAAA GGGCCACCTG 
CCCCTGGGAC AGAGCAACAG CTGTGGCAGC GTGCTCCCCC ACTCGCTAGC AACCATGACC 
AAAGACTCAG AATCTCGGAG GATGCTCAGA GGATATAATC ACCAACAGAT GAGTGAAGGA 
CAAAGGCAGA AGTCTTCTGA ATTTTGCAAC CGCACAGCAT CTGAATCGAA TGTCTACTTG 
AACAGTTTCC ACTACCCAGA TCACAGCTAC AAGGACCAGG CCTTTGATAC TCTGAGCCTA 
GACAGCTCTG ACAGCATGGA GACCAGCATC TCTGCCTGCT CACCTGACAA TATCTCTAGT 
GCCAGCACTT CAAATATTGC CCGAATAGAA GAAATGGAGA GACTGTTGAA GCAGGCTCAT 
GCAGAGAAGA CCAGGCTGCT TGAATCCAGG GAACGAGAAA TGGAAGCCAA GAAACGCGCT 
CTGGAAGAAG AAAAACGACG TCGAGAACTC CTGGAAAAAC GGTTGCAGGA AGAAACTAGC 
CAGAGGCAGA AGTTAATTGA AAAGGAAGTA AAAATAAGGG AGAAACAAAG GGCACAGGCT 
CGACCTCTGA CCCGCTATCT GCCTGTGCGG AAGGAAGACT TTGATTTGCG GAGCCACGTA 
GAGACTGCTG GCCACAATAT CGATACGTGT TATCATGTGT CAATCACAGA GAAGACCTGC 
AGAGGATTCC TCATCAAAAT GGGTGGAAAA ATTAAAACCT GGAAAAAACG TTGGTTTGTT 
TTTGATCGGA ACAAGCGCAC CTTCTCTTAT TATGCAGACA AGCATGAAGC TAAATTAAAA 
GGAGTAATAT ACTTTCAAGC CATTGAAGAA GTATATTATG ATCACCTCAA GAATGCTAAT 
AAGAGTCCTA ATCCTTTACT TACCTTTAGC GTCAAGACAC ATGACAGAAT CTACTACATG 
GTGGCCCCGT CACCAGAAGC CATGCGGATC TGGATGGACG TTATAGTGAC TGGGGCAGAA 
GGCTACACTC ACTTCTTGTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_006046551.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006046613.1 Bubalus bubalis pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu05334
Clone ID Related Accession (Same CDS sequence)	XM_006046551.1
Accession Version	XM_006046551.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783892.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAGC CACAAAGTAA AGAGCATTTT AGAAGTCTGG AAGAAAGGAA AAAACAGCAC 
CAGGAAGGCC TCTATCTGAG TGACACTTTG CCTCGAAAGA AAACCACACC TTCCATATCC 
CCACATTTCA GCAGTGCTAC TATGGGGAGA AGCACCACCC CAAAGGGCCA CCTGCCCCTG 
GGACAGAGCA ACAGCTGTGG CAGCGTGCTC CCCCACTCGC TAGCAACCAT GACCAAAGAC 
TCAGAATCTC GGAGGATGCT CAGAGGGAAG TCCAAGAAAG GAAGAATGAA TTTCTCAGCA 
GGAGCAGTTA GTAATTCCAA AAGTTCAAGT GTCAAAGGAT ATAATCACCA ACAGATGAGT 
GAAGGACAAA GGCAGAAGTC TTCTGAATTT TGCAACCGCA CAGCATCTGA ATCGAATGTC 
TACTTGAACA GTTTCCACTA CCCAGATCAC AGCTACAAGG ACCAGGCCTT TGATACTCTG 
AGCCTAGACA GCTCTGACAG CATGGAGACC AGCATCTCTG CCTGCTCACC TGACAATATC 
TCTAGTGCCA GCACTTCAAA TATTGCCCGA ATAGAAGAAA TGGAGAGACT GTTGAAGCAG 
GCTCATGCAG AGAAGACCAG GCTGCTTGAA TCCAGGGAAC GAGAAATGGA AGCCAAGAAA 
CGCGCTCTGG AAGAAGAAAA ACGACGTCGA GAACTCCTGG AAAAACGGTT GCAGGAAGAA 
ACTAGCCAGA GGCAGAAGTT AATTGAAAAG GAAGTAAAAA TAAGGGAGAA ACAAAGGGCA 
CAGGCTCGAC CTCTGACCCG CTATCTGCCT GTGCGGAAGG AAGACTTTGA TTTGCGGAGC 
CACGTAGAGA CTGCTGGCCA CAATATCGAT ACGTGTTATC ATGTGTCAAT CACAGAGAAG 
ACCTGCAGAG GATTCCTCAT CAAAATGGGT GGAAAAATTA AAACCTGGAA AAAACGTTGG 
TTTGTTTTTG ATCGGAACAA GCGCACCTTC TCTTATTATG CAGACAAGCA TGAAGCTAAA 
TTAAAAGGAG TAATATACTT TCAAGCCATT GAAGAAGTAT ATTATGATCA CCTCAAGAAT 
GCTAATAAGA GTCCTAATCC TTTACTTACC TTTAGCGTCA AGACACATGA CAGAATCTAC 
TACATGGTGG CCCCGTCACC AGAAGCCATG CGGATCTGGA TGGACGTTAT AGTGACTGGG 
GCAGAAGGCT ACACTCACTT CTTGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006046613.1
CDS	1080..2309
Translation	MSQPQSKEHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGKSKKGRMNFSAGAVSNSKSSSVKGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_006046551.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology-like domain, family B, member 2 (PHLDB2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006046551.1

ORF Insert Sequence:

ATGAGCCAGC CACAAAGTAA AGAGCATTTT AGAAGTCTGG AAGAAAGGAA AAAACAGCAC 
CAGGAAGGCC TCTATCTGAG TGACACTTTG CCTCGAAAGA AAACCACACC TTCCATATCC 
CCACATTTCA GCAGTGCTAC TATGGGGAGA AGCACCACCC CAAAGGGCCA CCTGCCCCTG 
GGACAGAGCA ACAGCTGTGG CAGCGTGCTC CCCCACTCGC TAGCAACCAT GACCAAAGAC 
TCAGAATCTC GGAGGATGCT CAGAGGGAAG TCCAAGAAAG GAAGAATGAA TTTCTCAGCA 
GGAGCAGTTA GTAATTCCAA AAGTTCAAGT GTCAAAGGAT ATAATCACCA ACAGATGAGT 
GAAGGACAAA GGCAGAAGTC TTCTGAATTT TGCAACCGCA CAGCATCTGA ATCGAATGTC 
TACTTGAACA GTTTCCACTA CCCAGATCAC AGCTACAAGG ACCAGGCCTT TGATACTCTG 
AGCCTAGACA GCTCTGACAG CATGGAGACC AGCATCTCTG CCTGCTCACC TGACAATATC 
TCTAGTGCCA GCACTTCAAA TATTGCCCGA ATAGAAGAAA TGGAGAGACT GTTGAAGCAG 
GCTCATGCAG AGAAGACCAG GCTGCTTGAA TCCAGGGAAC GAGAAATGGA AGCCAAGAAA 
CGCGCTCTGG AAGAAGAAAA ACGACGTCGA GAACTCCTGG AAAAACGGTT GCAGGAAGAA 
ACTAGCCAGA GGCAGAAGTT AATTGAAAAG GAAGTAAAAA TAAGGGAGAA ACAAAGGGCA 
CAGGCTCGAC CTCTGACCCG CTATCTGCCT GTGCGGAAGG AAGACTTTGA TTTGCGGAGC 
CACGTAGAGA CTGCTGGCCA CAATATCGAT ACGTGTTATC ATGTGTCAAT CACAGAGAAG 
ACCTGCAGAG GATTCCTCAT CAAAATGGGT GGAAAAATTA AAACCTGGAA AAAACGTTGG 
TTTGTTTTTG ATCGGAACAA GCGCACCTTC TCTTATTATG CAGACAAGCA TGAAGCTAAA 
TTAAAAGGAG TAATATACTT TCAAGCCATT GAAGAAGTAT ATTATGATCA CCTCAAGAAT 
GCTAATAAGA GTCCTAATCC TTTACTTACC TTTAGCGTCA AGACACATGA CAGAATCTAC 
TACATGGTGG CCCCGTCACC AGAAGCCATG CGGATCTGGA TGGACGTTAT AGTGACTGGG 
GCAGAAGGCT ACACTCACTT CTTGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283816.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139601.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu42732
Clone ID Related Accession (Same CDS sequence)	XM_025283816.1
Accession Version	XM_025283816.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT 
GTAAATGAGA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTT 
CCTGCTGATG CTGCTGCTGC TGCCACTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA 
CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT 
TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCTGTGGC TACCTCTGTC 
GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA 
CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA 
GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT 
TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG 
AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA 
TCTCGGAGGA TGCTCAGAGG GAAGTCCAAG AAAGGAAGAA TGAATTTCTC AGCAGGAGCA 
GTTAGTAATT CCAAAAGTTC AAGTGTCAAA GGATATAATC ACCAACAGAT GAGTGAAGGA 
CAAAGGCAGA AGTCTTCTGA ATTTTGCAAC CGCACAGCAT CTGAATCGAA TGTCTACTTG 
AACAGTTTCC ACTACCCAGA TCACAGCTAC AAGGACCAGG CCTTTGATAC TCTGAGCCTA 
GACAGCTCTG ACAGCATGGA GACCAGCATC TCTGCCTGCT CACCTGACAA TATCTCTAGT 
GCCAGCACTT CAAATATTGC CCGAATAGAA GAAATGGAGA GACTGTTGAA GCAGGCTCAT 
GCAGAGAAGA CCAGGCTGCT TGAATCCAGG GAACGAGAAA TGGAAGCCAA GAAACGCGCT 
CTGGAAGAAG AAAAACGACG TCGAGAACTC CTGGAAAAAC GGTTGCAGGA AGAAACTAGC 
CAGAGGCAGA AGTTAATTGA AAAGGAAGTA AAAATAAGGG AGAAACAAAG GGCACAGGCT 
CGACCTCTGA CCCGCTATCT GCCTGTGCGG AAGGAAGACT TTGATTTGCG GAGCCACGTA 
GAGACTGCTG GCCACAATAT CGATACGTGT TATCATGTGT CAATCACAGA GAAGACCTGC 
AGAGGATTCC TCATCAAAAT GGGTGGAAAA ATTAAAACCT GGAAAAAACG TTGGTTTGTT 
TTTGATCGGA ACAAGCGCAC CTTCTCTTAT TATGCAGACA AGCATGAAGC TAAATTAAAA 
GGAGTAATAT ACTTTCAAGC CATTGAAGAA GTATATTATG ATCACCTCAA GAACGCTAAT 
AAGAGTCCTA ATCCTTTACT TACCTTTAGC GTCAAGACAC ATGACAGAAT CTACTACATG 
GTGGCCCCGT CACCAGAAGC CATGCGGATC TGGATGGACG TTATAGTGAC TGGGGCAGAA 
GGCTACACTC ACTTCTTGTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139601.1
CDS	367..4470
Translation	MEEDNTQRKDTKEEGVGDRQPVDSNKIMAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEGYIIVNEINESCGSSTNLPPSTQFPADAAAAATGPTTAVLMSQPQSKEQRSSVCPGFMFPSTLSSHPITQPPSAPWPEAVATSVDSFPLNDTPPPLPAKKHRRQQQQDQQHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGKSKKGRMNFSAGAVSNSKSSSVKGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283816.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283816.1

ORF Insert Sequence:

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT 
GTAAATGAGA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTT 
CCTGCTGATG CTGCTGCTGC TGCCACTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA 
CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT 
TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCTGTGGC TACCTCTGTC 
GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA 
CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA 
GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT 
TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG 
AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA 
TCTCGGAGGA TGCTCAGAGG GAAGTCCAAG AAAGGAAGAA TGAATTTCTC AGCAGGAGCA 
GTTAGTAATT CCAAAAGTTC AAGTGTCAAA GGATATAATC ACCAACAGAT GAGTGAAGGA 
CAAAGGCAGA AGTCTTCTGA ATTTTGCAAC CGCACAGCAT CTGAATCGAA TGTCTACTTG 
AACAGTTTCC ACTACCCAGA TCACAGCTAC AAGGACCAGG CCTTTGATAC TCTGAGCCTA 
GACAGCTCTG ACAGCATGGA GACCAGCATC TCTGCCTGCT CACCTGACAA TATCTCTAGT 
GCCAGCACTT CAAATATTGC CCGAATAGAA GAAATGGAGA GACTGTTGAA GCAGGCTCAT 
GCAGAGAAGA CCAGGCTGCT TGAATCCAGG GAACGAGAAA TGGAAGCCAA GAAACGCGCT 
CTGGAAGAAG AAAAACGACG TCGAGAACTC CTGGAAAAAC GGTTGCAGGA AGAAACTAGC 
CAGAGGCAGA AGTTAATTGA AAAGGAAGTA AAAATAAGGG AGAAACAAAG GGCACAGGCT 
CGACCTCTGA CCCGCTATCT GCCTGTGCGG AAGGAAGACT TTGATTTGCG GAGCCACGTA 
GAGACTGCTG GCCACAATAT CGATACGTGT TATCATGTGT CAATCACAGA GAAGACCTGC 
AGAGGATTCC TCATCAAAAT GGGTGGAAAA ATTAAAACCT GGAAAAAACG TTGGTTTGTT 
TTTGATCGGA ACAAGCGCAC CTTCTCTTAT TATGCAGACA AGCATGAAGC TAAATTAAAA 
GGAGTAATAT ACTTTCAAGC CATTGAAGAA GTATATTATG ATCACCTCAA GAACGCTAAT 
AAGAGTCCTA ATCCTTTACT TACCTTTAGC GTCAAGACAC ATGACAGAAT CTACTACATG 
GTGGCCCCGT CACCAGAAGC CATGCGGATC TGGATGGACG TTATAGTGAC TGGGGCAGAA 
GGCTACACTC ACTTCTTGTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283820.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139605.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X2, mRNA.

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CloneID	OBu42733
Clone ID Related Accession (Same CDS sequence)	XM_025283820.1
Accession Version	XM_025283820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT 
GTAAATGAGA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTT 
CCTGCTGATG CTGCTGCTGC TGCCACTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA 
CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT 
TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCTGTGGC TACCTCTGTC 
GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA 
CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA 
GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT 
TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG 
AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA 
TCTCGGAGGA TGCTCAGAGG AGCAGTTAGT AATTCCAAAA GTTCAAGTGT CAAAGGATAT 
AATCACCAAC AGATGAGTGA AGGACAAAGG CAGAAGTCTT CTGAATTTTG CAACCGCACA 
GCATCTGAAT CGAATGTCTA CTTGAACAGT TTCCACTACC CAGATCACAG CTACAAGGAC 
CAGGCCTTTG ATACTCTGAG CCTAGACAGC TCTGACAGCA TGGAGACCAG CATCTCTGCC 
TGCTCACCTG ACAATATCTC TAGTGCCAGC ACTTCAAATA TTGCCCGAAT AGAAGAAATG 
GAGAGACTGT TGAAGCAGGC TCATGCAGAG AAGACCAGGC TGCTTGAATC CAGGGAACGA 
GAAATGGAAG CCAAGAAACG CGCTCTGGAA GAAGAAAAAC GACGTCGAGA ACTCCTGGAA 
AAACGGTTGC AGGAAGAAAC TAGCCAGAGG CAGAAGTTAA TTGAAAAGGA AGTAAAAATA 
AGGGAGAAAC AAAGGGCACA GGCTCGACCT CTGACCCGCT ATCTGCCTGT GCGGAAGGAA 
GACTTTGATT TGCGGAGCCA CGTAGAGACT GCTGGCCACA ATATCGATAC GTGTTATCAT 
GTGTCAATCA CAGAGAAGAC CTGCAGAGGA TTCCTCATCA AAATGGGTGG AAAAATTAAA 
ACCTGGAAAA AACGTTGGTT TGTTTTTGAT CGGAACAAGC GCACCTTCTC TTATTATGCA 
GACAAGCATG AAGCTAAATT AAAAGGAGTA ATATACTTTC AAGCCATTGA AGAAGTATAT 
TATGATCACC TCAAGAACGC TAATAAGAGT CCTAATCCTT TACTTACCTT TAGCGTCAAG 
ACACATGACA GAATCTACTA CATGGTGGCC CCGTCACCAG AAGCCATGCG GATCTGGATG 
GACGTTATAG TGACTGGGGC AGAAGGCTAC ACTCACTTCT TGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139605.1
CDS	366..4433
Translation	MEEDNTQRKDTKEEGVGDRQPVDSNKIMAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEGYIIVNEINESCGSSTNLPPSTQFPADAAAAATGPTTAVLMSQPQSKEQRSSVCPGFMFPSTLSSHPITQPPSAPWPEAVATSVDSFPLNDTPPPLPAKKHRRQQQQDQQHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGAVSNSKSSSVKGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283820.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283820.1

ORF Insert Sequence:

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT 
GTAAATGAGA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTT 
CCTGCTGATG CTGCTGCTGC TGCCACTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA 
CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT 
TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCTGTGGC TACCTCTGTC 
GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA 
CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA 
GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT 
TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG 
AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA 
TCTCGGAGGA TGCTCAGAGG AGCAGTTAGT AATTCCAAAA GTTCAAGTGT CAAAGGATAT 
AATCACCAAC AGATGAGTGA AGGACAAAGG CAGAAGTCTT CTGAATTTTG CAACCGCACA 
GCATCTGAAT CGAATGTCTA CTTGAACAGT TTCCACTACC CAGATCACAG CTACAAGGAC 
CAGGCCTTTG ATACTCTGAG CCTAGACAGC TCTGACAGCA TGGAGACCAG CATCTCTGCC 
TGCTCACCTG ACAATATCTC TAGTGCCAGC ACTTCAAATA TTGCCCGAAT AGAAGAAATG 
GAGAGACTGT TGAAGCAGGC TCATGCAGAG AAGACCAGGC TGCTTGAATC CAGGGAACGA 
GAAATGGAAG CCAAGAAACG CGCTCTGGAA GAAGAAAAAC GACGTCGAGA ACTCCTGGAA 
AAACGGTTGC AGGAAGAAAC TAGCCAGAGG CAGAAGTTAA TTGAAAAGGA AGTAAAAATA 
AGGGAGAAAC AAAGGGCACA GGCTCGACCT CTGACCCGCT ATCTGCCTGT GCGGAAGGAA 
GACTTTGATT TGCGGAGCCA CGTAGAGACT GCTGGCCACA ATATCGATAC GTGTTATCAT 
GTGTCAATCA CAGAGAAGAC CTGCAGAGGA TTCCTCATCA AAATGGGTGG AAAAATTAAA 
ACCTGGAAAA AACGTTGGTT TGTTTTTGAT CGGAACAAGC GCACCTTCTC TTATTATGCA 
GACAAGCATG AAGCTAAATT AAAAGGAGTA ATATACTTTC AAGCCATTGA AGAAGTATAT 
TATGATCACC TCAAGAACGC TAATAAGAGT CCTAATCCTT TACTTACCTT TAGCGTCAAG 
ACACATGACA GAATCTACTA CATGGTGGCC CCGTCACCAG AAGCCATGCG GATCTGGATG 
GACGTTATAG TGACTGGGGC AGAAGGCTAC ACTCACTTCT TGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283825.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139610.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu42734
Clone ID Related Accession (Same CDS sequence)	XM_025283825.1
Accession Version	XM_025283825.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT 
GTAAATGAGA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTT 
CCTGCTGATG CTGCTGCTGC TGCCACTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA 
CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT 
TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCTGTGGC TACCTCTGTC 
GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA 
CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA 
GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT 
TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG 
AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA 
TCTCGGAGGA TGCTCAGAGG GAAGTCCAAG AAAGGAAGAA TGAATTTCTC AGCAGGATAT 
AATCACCAAC AGATGAGTGA AGGACAAAGG CAGAAGTCTT CTGAATTTTG CAACCGCACA 
GCATCTGAAT CGAATGTCTA CTTGAACAGT TTCCACTACC CAGATCACAG CTACAAGGAC 
CAGGCCTTTG ATACTCTGAG CCTAGACAGC TCTGACAGCA TGGAGACCAG CATCTCTGCC 
TGCTCACCTG ACAATATCTC TAGTGCCAGC ACTTCAAATA TTGCCCGAAT AGAAGAAATG 
GAGAGACTGT TGAAGCAGGC TCATGCAGAG AAGACCAGGC TGCTTGAATC CAGGGAACGA 
GAAATGGAAG CCAAGAAACG CGCTCTGGAA GAAGAAAAAC GACGTCGAGA ACTCCTGGAA 
AAACGGTTGC AGGAAGAAAC TAGCCAGAGG CAGAAGTTAA TTGAAAAGGA AGTAAAAATA 
AGGGAGAAAC AAAGGGCACA GGCTCGACCT CTGACCCGCT ATCTGCCTGT GCGGAAGGAA 
GACTTTGATT TGCGGAGCCA CGTAGAGACT GCTGGCCACA ATATCGATAC GTGTTATCAT 
GTGTCAATCA CAGAGAAGAC CTGCAGAGGA TTCCTCATCA AAATGGGTGG AAAAATTAAA 
ACCTGGAAAA AACGTTGGTT TGTTTTTGAT CGGAACAAGC GCACCTTCTC TTATTATGCA 
GACAAGCATG AAGCTAAATT AAAAGGAGTA ATATACTTTC AAGCCATTGA AGAAGTATAT 
TATGATCACC TCAAGAACGC TAATAAGAGT CCTAATCCTT TACTTACCTT TAGCGTCAAG 
ACACATGACA GAATCTACTA CATGGTGGCC CCGTCACCAG AAGCCATGCG GATCTGGATG 
GACGTTATAG TGACTGGGGC AGAAGGCTAC ACTCACTTCT TGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139610.1
CDS	366..4433
Translation	MEEDNTQRKDTKEEGVGDRQPVDSNKIMAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEGYIIVNEINESCGSSTNLPPSTQFPADAAAAATGPTTAVLMSQPQSKEQRSSVCPGFMFPSTLSSHPITQPPSAPWPEAVATSVDSFPLNDTPPPLPAKKHRRQQQQDQQHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGKSKKGRMNFSAGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283825.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283825.1

ORF Insert Sequence:

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT 
GTAAATGAGA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTT 
CCTGCTGATG CTGCTGCTGC TGCCACTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA 
CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT 
TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCTGTGGC TACCTCTGTC 
GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA 
CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA 
GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT 
TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG 
AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA 
TCTCGGAGGA TGCTCAGAGG GAAGTCCAAG AAAGGAAGAA TGAATTTCTC AGCAGGATAT 
AATCACCAAC AGATGAGTGA AGGACAAAGG CAGAAGTCTT CTGAATTTTG CAACCGCACA 
GCATCTGAAT CGAATGTCTA CTTGAACAGT TTCCACTACC CAGATCACAG CTACAAGGAC 
CAGGCCTTTG ATACTCTGAG CCTAGACAGC TCTGACAGCA TGGAGACCAG CATCTCTGCC 
TGCTCACCTG ACAATATCTC TAGTGCCAGC ACTTCAAATA TTGCCCGAAT AGAAGAAATG 
GAGAGACTGT TGAAGCAGGC TCATGCAGAG AAGACCAGGC TGCTTGAATC CAGGGAACGA 
GAAATGGAAG CCAAGAAACG CGCTCTGGAA GAAGAAAAAC GACGTCGAGA ACTCCTGGAA 
AAACGGTTGC AGGAAGAAAC TAGCCAGAGG CAGAAGTTAA TTGAAAAGGA AGTAAAAATA 
AGGGAGAAAC AAAGGGCACA GGCTCGACCT CTGACCCGCT ATCTGCCTGT GCGGAAGGAA 
GACTTTGATT TGCGGAGCCA CGTAGAGACT GCTGGCCACA ATATCGATAC GTGTTATCAT 
GTGTCAATCA CAGAGAAGAC CTGCAGAGGA TTCCTCATCA AAATGGGTGG AAAAATTAAA 
ACCTGGAAAA AACGTTGGTT TGTTTTTGAT CGGAACAAGC GCACCTTCTC TTATTATGCA 
GACAAGCATG AAGCTAAATT AAAAGGAGTA ATATACTTTC AAGCCATTGA AGAAGTATAT 
TATGATCACC TCAAGAACGC TAATAAGAGT CCTAATCCTT TACTTACCTT TAGCGTCAAG 
ACACATGACA GAATCTACTA CATGGTGGCC CCGTCACCAG AAGCCATGCG GATCTGGATG 
GACGTTATAG TGACTGGGGC AGAAGGCTAC ACTCACTTCT TGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283834.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139619.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X4, mRNA.

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CloneID	OBu42735
Clone ID Related Accession (Same CDS sequence)	XM_025283834.1
Accession Version	XM_025283834.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT 
GTAAATGAGA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTT 
CCTGCTGATG CTGCTGCTGC TGCCACTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA 
CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT 
TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCTGTGGC TACCTCTGTC 
GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA 
CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA 
GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT 
TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG 
AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA 
TCTCGGAGGA TGCTCAGAGG ATATAATCAC CAACAGATGA GTGAAGGACA AAGGCAGAAG 
TCTTCTGAAT TTTGCAACCG CACAGCATCT GAATCGAATG TCTACTTGAA CAGTTTCCAC 
TACCCAGATC ACAGCTACAA GGACCAGGCC TTTGATACTC TGAGCCTAGA CAGCTCTGAC 
AGCATGGAGA CCAGCATCTC TGCCTGCTCA CCTGACAATA TCTCTAGTGC CAGCACTTCA 
AATATTGCCC GAATAGAAGA AATGGAGAGA CTGTTGAAGC AGGCTCATGC AGAGAAGACC 
AGGCTGCTTG AATCCAGGGA ACGAGAAATG GAAGCCAAGA AACGCGCTCT GGAAGAAGAA 
AAACGACGTC GAGAACTCCT GGAAAAACGG TTGCAGGAAG AAACTAGCCA GAGGCAGAAG 
TTAATTGAAA AGGAAGTAAA AATAAGGGAG AAACAAAGGG CACAGGCTCG ACCTCTGACC 
CGCTATCTGC CTGTGCGGAA GGAAGACTTT GATTTGCGGA GCCACGTAGA GACTGCTGGC 
CACAATATCG ATACGTGTTA TCATGTGTCA ATCACAGAGA AGACCTGCAG AGGATTCCTC 
ATCAAAATGG GTGGAAAAAT TAAAACCTGG AAAAAACGTT GGTTTGTTTT TGATCGGAAC 
AAGCGCACCT TCTCTTATTA TGCAGACAAG CATGAAGCTA AATTAAAAGG AGTAATATAC 
TTTCAAGCCA TTGAAGAAGT ATATTATGAT CACCTCAAGA ACGCTAATAA GAGTCCTAAT 
CCTTTACTTA CCTTTAGCGT CAAGACACAT GACAGAATCT ACTACATGGT GGCCCCGTCA 
CCAGAAGCCA TGCGGATCTG GATGGACGTT ATAGTGACTG GGGCAGAAGG CTACACTCAC 
TTCTTGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139619.1
CDS	362..4393
Translation	MEEDNTQRKDTKEEGVGDRQPVDSNKIMAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEGYIIVNEINESCGSSTNLPPSTQFPADAAAAATGPTTAVLMSQPQSKEQRSSVCPGFMFPSTLSSHPITQPPSAPWPEAVATSVDSFPLNDTPPPLPAKKHRRQQQQDQQHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283834.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283834.1

ORF Insert Sequence:

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT 
GTAAATGAGA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTT 
CCTGCTGATG CTGCTGCTGC TGCCACTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA 
CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT 
TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCTGTGGC TACCTCTGTC 
GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA 
CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA 
GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT 
TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG 
AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA 
TCTCGGAGGA TGCTCAGAGG ATATAATCAC CAACAGATGA GTGAAGGACA AAGGCAGAAG 
TCTTCTGAAT TTTGCAACCG CACAGCATCT GAATCGAATG TCTACTTGAA CAGTTTCCAC 
TACCCAGATC ACAGCTACAA GGACCAGGCC TTTGATACTC TGAGCCTAGA CAGCTCTGAC 
AGCATGGAGA CCAGCATCTC TGCCTGCTCA CCTGACAATA TCTCTAGTGC CAGCACTTCA 
AATATTGCCC GAATAGAAGA AATGGAGAGA CTGTTGAAGC AGGCTCATGC AGAGAAGACC 
AGGCTGCTTG AATCCAGGGA ACGAGAAATG GAAGCCAAGA AACGCGCTCT GGAAGAAGAA 
AAACGACGTC GAGAACTCCT GGAAAAACGG TTGCAGGAAG AAACTAGCCA GAGGCAGAAG 
TTAATTGAAA AGGAAGTAAA AATAAGGGAG AAACAAAGGG CACAGGCTCG ACCTCTGACC 
CGCTATCTGC CTGTGCGGAA GGAAGACTTT GATTTGCGGA GCCACGTAGA GACTGCTGGC 
CACAATATCG ATACGTGTTA TCATGTGTCA ATCACAGAGA AGACCTGCAG AGGATTCCTC 
ATCAAAATGG GTGGAAAAAT TAAAACCTGG AAAAAACGTT GGTTTGTTTT TGATCGGAAC 
AAGCGCACCT TCTCTTATTA TGCAGACAAG CATGAAGCTA AATTAAAAGG AGTAATATAC 
TTTCAAGCCA TTGAAGAAGT ATATTATGAT CACCTCAAGA ACGCTAATAA GAGTCCTAAT 
CCTTTACTTA CCTTTAGCGT CAAGACACAT GACAGAATCT ACTACATGGT GGCCCCGTCA 
CCAGAAGCCA TGCGGATCTG GATGGACGTT ATAGTGACTG GGGCAGAAGG CTACACTCAC 
TTCTTGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283861.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139646.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X8, mRNA.

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CloneID	OBu42736
Clone ID Related Accession (Same CDS sequence)	XM_025283861.1 , XM_025283839.1 , XM_025283846.1 , XM_025283855.1 , XM_025283866.1 , XM_025283868.1
Accession Version	XM_025283861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AGAGCCACAT GCAGAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
GGCTTTGTGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA 
AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACAAAGCAGT TCTCTTGCGA TGGAAGTGAC 
AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA 
TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG 
AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT 
GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG 
AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCGCCCATCA ACAGGTCAGG GGCGGCCAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG 
CCCAAGTTGA GCAGACACCG GGACCCGGCA TCTGAAAACA TCAGTTTAAG AACCAGGAAG 
TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT 
AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT 
TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATA GCATTATAGA CAATGACAAT 
TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG 
GGCAGTCCGT ACGTGAGTTC CACCCTCAGT GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG 
GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCGTT TGCAGCCAGA 
AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC 
GGACGAGACG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG 
GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC 
ACAAAGCCTG ACAGTCGCGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT 
AAAGAGCTGG AGAAACTGCA GCTCTCCGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG 
AGCCCGGACA CCAGGTATAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA 
GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CTCCAGGAAT 
GATGAACTGC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA 
GCTTCCGGCG AGTCTGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC 
CTTGAAGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA 
GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT 
ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA 
CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG 
GAGAACCTGA CCCAGCAGCT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA 
CGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA 
AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAGAT TAATGAGTCA 
TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTTC CTGCTGATGC TGCTGCTGCT 
GCCACTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG 
TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT 
CCATCAGCCC CTTGGCCTGA AGCTGTGGCT ACCTCTGTCG ATTCTTTTCC TTTAAATGAC 
ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT 
TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT 
TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG 
AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG 
CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGG 
AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGTTCA 
AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA 
TTTTGCAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT 
CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG 
ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC 
CGAATAGAAG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC CAGGCTGCTT 
GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT 
CGAGAACTCC TGGAAAAACG GTTGCAGGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA 
AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG 
CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC 
GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG 
GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC 
TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC 
ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT 
ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC ACCAGAAGCC 
ATGCGGATCT GGATGGACGT TATAGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139646.1
CDS	128..4150
Translation	MAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEGYIIVNEINESCGSSTNLPPSTQFPADAAAAATGPTTAVLMSQPQSKEQRSSVCPGFMFPSTLSSHPITQPPSAPWPEAVATSVDSFPLNDTPPPLPAKKHRRQQQQDQQHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGKSKKGRMNFSAGAVSNSKSSSVKGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283861.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283861.1

ORF Insert Sequence:

ATGGCAGAGC AGAGCCACAT GCAGAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
GGCTTTGTGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA 
AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACAAAGCAGT TCTCTTGCGA TGGAAGTGAC 
AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA 
TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG 
AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT 
GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG 
AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCGCCCATCA ACAGGTCAGG GGCGGCCAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG 
CCCAAGTTGA GCAGACACCG GGACCCGGCA TCTGAAAACA TCAGTTTAAG AACCAGGAAG 
TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT 
AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT 
TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATA GCATTATAGA CAATGACAAT 
TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG 
GGCAGTCCGT ACGTGAGTTC CACCCTCAGT GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG 
GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCGTT TGCAGCCAGA 
AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC 
GGACGAGACG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG 
GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC 
ACAAAGCCTG ACAGTCGCGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT 
AAAGAGCTGG AGAAACTGCA GCTCTCCGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG 
AGCCCGGACA CCAGGTATAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA 
GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CTCCAGGAAT 
GATGAACTGC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA 
GCTTCCGGCG AGTCTGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC 
CTTGAAGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA 
GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT 
ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA 
CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG 
GAGAACCTGA CCCAGCAGCT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA 
CGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA 
AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAGAT TAATGAGTCA 
TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTTC CTGCTGATGC TGCTGCTGCT 
GCCACTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG 
TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT 
CCATCAGCCC CTTGGCCTGA AGCTGTGGCT ACCTCTGTCG ATTCTTTTCC TTTAAATGAC 
ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT 
TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT 
TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG 
AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG 
CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGG 
AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGTTCA 
AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA 
TTTTGCAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT 
CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG 
ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC 
CGAATAGAAG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC CAGGCTGCTT 
GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT 
CGAGAACTCC TGGAAAAACG GTTGCAGGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA 
AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG 
CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC 
GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG 
GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC 
TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC 
ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT 
ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC ACCAGAAGCC 
ATGCGGATCT GGATGGACGT TATAGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283839.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139624.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu42736
Clone ID Related Accession (Same CDS sequence)	XM_025283861.1 , XM_025283839.1 , XM_025283846.1 , XM_025283855.1 , XM_025283866.1 , XM_025283868.1
Accession Version	XM_025283839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AGAGCCACAT GCAGAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
GGCTTTGTGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA 
AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACAAAGCAGT TCTCTTGCGA TGGAAGTGAC 
AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA 
TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG 
AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT 
GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG 
AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCGCCCATCA ACAGGTCAGG GGCGGCCAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG 
CCCAAGTTGA GCAGACACCG GGACCCGGCA TCTGAAAACA TCAGTTTAAG AACCAGGAAG 
TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT 
AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT 
TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATA GCATTATAGA CAATGACAAT 
TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG 
GGCAGTCCGT ACGTGAGTTC CACCCTCAGT GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG 
GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCGTT TGCAGCCAGA 
AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC 
GGACGAGACG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG 
GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC 
ACAAAGCCTG ACAGTCGCGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT 
AAAGAGCTGG AGAAACTGCA GCTCTCCGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG 
AGCCCGGACA CCAGGTATAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA 
GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CTCCAGGAAT 
GATGAACTGC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA 
GCTTCCGGCG AGTCTGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC 
CTTGAAGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA 
GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT 
ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA 
CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG 
GAGAACCTGA CCCAGCAGCT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA 
CGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA 
AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAGAT TAATGAGTCA 
TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTTC CTGCTGATGC TGCTGCTGCT 
GCCACTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG 
TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT 
CCATCAGCCC CTTGGCCTGA AGCTGTGGCT ACCTCTGTCG ATTCTTTTCC TTTAAATGAC 
ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT 
TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT 
TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG 
AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG 
CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGG 
AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGTTCA 
AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA 
TTTTGCAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT 
CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG 
ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC 
CGAATAGAAG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC CAGGCTGCTT 
GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT 
CGAGAACTCC TGGAAAAACG GTTGCAGGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA 
AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG 
CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC 
GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG 
GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC 
TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC 
ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT 
ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC ACCAGAAGCC 
ATGCGGATCT GGATGGACGT TATAGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139624.1
CDS	150..4172
Translation	MAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEGYIIVNEINESCGSSTNLPPSTQFPADAAAAATGPTTAVLMSQPQSKEQRSSVCPGFMFPSTLSSHPITQPPSAPWPEAVATSVDSFPLNDTPPPLPAKKHRRQQQQDQQHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGKSKKGRMNFSAGAVSNSKSSSVKGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283839.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283839.1

ORF Insert Sequence:

ATGGCAGAGC AGAGCCACAT GCAGAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
GGCTTTGTGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA 
AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACAAAGCAGT TCTCTTGCGA TGGAAGTGAC 
AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA 
TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG 
AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT 
GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG 
AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCGCCCATCA ACAGGTCAGG GGCGGCCAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG 
CCCAAGTTGA GCAGACACCG GGACCCGGCA TCTGAAAACA TCAGTTTAAG AACCAGGAAG 
TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT 
AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT 
TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATA GCATTATAGA CAATGACAAT 
TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG 
GGCAGTCCGT ACGTGAGTTC CACCCTCAGT GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG 
GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCGTT TGCAGCCAGA 
AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC 
GGACGAGACG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG 
GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC 
ACAAAGCCTG ACAGTCGCGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT 
AAAGAGCTGG AGAAACTGCA GCTCTCCGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG 
AGCCCGGACA CCAGGTATAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA 
GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CTCCAGGAAT 
GATGAACTGC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA 
GCTTCCGGCG AGTCTGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC 
CTTGAAGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA 
GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT 
ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA 
CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG 
GAGAACCTGA CCCAGCAGCT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA 
CGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA 
AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAGAT TAATGAGTCA 
TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTTC CTGCTGATGC TGCTGCTGCT 
GCCACTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG 
TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT 
CCATCAGCCC CTTGGCCTGA AGCTGTGGCT ACCTCTGTCG ATTCTTTTCC TTTAAATGAC 
ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT 
TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT 
TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG 
AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG 
CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGG 
AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGTTCA 
AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA 
TTTTGCAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT 
CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG 
ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC 
CGAATAGAAG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC CAGGCTGCTT 
GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT 
CGAGAACTCC TGGAAAAACG GTTGCAGGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA 
AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG 
CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC 
GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG 
GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC 
TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC 
ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT 
ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC ACCAGAAGCC 
ATGCGGATCT GGATGGACGT TATAGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283846.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139631.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X6, mRNA.

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CloneID	OBu42736
Clone ID Related Accession (Same CDS sequence)	XM_025283861.1 , XM_025283839.1 , XM_025283846.1 , XM_025283855.1 , XM_025283866.1 , XM_025283868.1
Accession Version	XM_025283846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AGAGCCACAT GCAGAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
GGCTTTGTGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA 
AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACAAAGCAGT TCTCTTGCGA TGGAAGTGAC 
AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA 
TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG 
AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT 
GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG 
AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCGCCCATCA ACAGGTCAGG GGCGGCCAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG 
CCCAAGTTGA GCAGACACCG GGACCCGGCA TCTGAAAACA TCAGTTTAAG AACCAGGAAG 
TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT 
AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT 
TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATA GCATTATAGA CAATGACAAT 
TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG 
GGCAGTCCGT ACGTGAGTTC CACCCTCAGT GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG 
GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCGTT TGCAGCCAGA 
AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC 
GGACGAGACG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG 
GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC 
ACAAAGCCTG ACAGTCGCGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT 
AAAGAGCTGG AGAAACTGCA GCTCTCCGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG 
AGCCCGGACA CCAGGTATAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA 
GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CTCCAGGAAT 
GATGAACTGC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA 
GCTTCCGGCG AGTCTGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC 
CTTGAAGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA 
GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT 
ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA 
CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG 
GAGAACCTGA CCCAGCAGCT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA 
CGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA 
AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAGAT TAATGAGTCA 
TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTTC CTGCTGATGC TGCTGCTGCT 
GCCACTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG 
TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT 
CCATCAGCCC CTTGGCCTGA AGCTGTGGCT ACCTCTGTCG ATTCTTTTCC TTTAAATGAC 
ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT 
TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT 
TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG 
AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG 
CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGG 
AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGTTCA 
AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA 
TTTTGCAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT 
CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG 
ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC 
CGAATAGAAG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC CAGGCTGCTT 
GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT 
CGAGAACTCC TGGAAAAACG GTTGCAGGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA 
AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG 
CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC 
GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG 
GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC 
TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC 
ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT 
ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC ACCAGAAGCC 
ATGCGGATCT GGATGGACGT TATAGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139631.1
CDS	404..4426
Translation	MAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEGYIIVNEINESCGSSTNLPPSTQFPADAAAAATGPTTAVLMSQPQSKEQRSSVCPGFMFPSTLSSHPITQPPSAPWPEAVATSVDSFPLNDTPPPLPAKKHRRQQQQDQQHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGKSKKGRMNFSAGAVSNSKSSSVKGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283846.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283846.1

ORF Insert Sequence:

ATGGCAGAGC AGAGCCACAT GCAGAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
GGCTTTGTGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA 
AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACAAAGCAGT TCTCTTGCGA TGGAAGTGAC 
AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA 
TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG 
AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT 
GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG 
AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCGCCCATCA ACAGGTCAGG GGCGGCCAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG 
CCCAAGTTGA GCAGACACCG GGACCCGGCA TCTGAAAACA TCAGTTTAAG AACCAGGAAG 
TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT 
AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT 
TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATA GCATTATAGA CAATGACAAT 
TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG 
GGCAGTCCGT ACGTGAGTTC CACCCTCAGT GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG 
GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCGTT TGCAGCCAGA 
AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC 
GGACGAGACG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG 
GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC 
ACAAAGCCTG ACAGTCGCGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT 
AAAGAGCTGG AGAAACTGCA GCTCTCCGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG 
AGCCCGGACA CCAGGTATAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA 
GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CTCCAGGAAT 
GATGAACTGC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA 
GCTTCCGGCG AGTCTGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC 
CTTGAAGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA 
GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT 
ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA 
CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG 
GAGAACCTGA CCCAGCAGCT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA 
CGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA 
AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAGAT TAATGAGTCA 
TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTTC CTGCTGATGC TGCTGCTGCT 
GCCACTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG 
TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT 
CCATCAGCCC CTTGGCCTGA AGCTGTGGCT ACCTCTGTCG ATTCTTTTCC TTTAAATGAC 
ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT 
TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT 
TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG 
AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG 
CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGG 
AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGTTCA 
AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA 
TTTTGCAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT 
CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG 
ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC 
CGAATAGAAG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC CAGGCTGCTT 
GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT 
CGAGAACTCC TGGAAAAACG GTTGCAGGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA 
AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG 
CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC 
GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG 
GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC 
TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC 
ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT 
ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC ACCAGAAGCC 
ATGCGGATCT GGATGGACGT TATAGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283855.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139640.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X7, mRNA.

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CloneID	OBu42736
Clone ID Related Accession (Same CDS sequence)	XM_025283861.1 , XM_025283839.1 , XM_025283846.1 , XM_025283855.1 , XM_025283866.1 , XM_025283868.1
Accession Version	XM_025283855.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AGAGCCACAT GCAGAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
GGCTTTGTGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA 
AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACAAAGCAGT TCTCTTGCGA TGGAAGTGAC 
AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA 
TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG 
AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT 
GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG 
AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCGCCCATCA ACAGGTCAGG GGCGGCCAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG 
CCCAAGTTGA GCAGACACCG GGACCCGGCA TCTGAAAACA TCAGTTTAAG AACCAGGAAG 
TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT 
AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT 
TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATA GCATTATAGA CAATGACAAT 
TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG 
GGCAGTCCGT ACGTGAGTTC CACCCTCAGT GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG 
GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCGTT TGCAGCCAGA 
AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC 
GGACGAGACG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG 
GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC 
ACAAAGCCTG ACAGTCGCGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT 
AAAGAGCTGG AGAAACTGCA GCTCTCCGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG 
AGCCCGGACA CCAGGTATAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA 
GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CTCCAGGAAT 
GATGAACTGC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA 
GCTTCCGGCG AGTCTGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC 
CTTGAAGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA 
GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT 
ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA 
CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG 
GAGAACCTGA CCCAGCAGCT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA 
CGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA 
AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAGAT TAATGAGTCA 
TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTTC CTGCTGATGC TGCTGCTGCT 
GCCACTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG 
TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT 
CCATCAGCCC CTTGGCCTGA AGCTGTGGCT ACCTCTGTCG ATTCTTTTCC TTTAAATGAC 
ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT 
TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT 
TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG 
AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG 
CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGG 
AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGTTCA 
AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA 
TTTTGCAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT 
CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG 
ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC 
CGAATAGAAG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC CAGGCTGCTT 
GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT 
CGAGAACTCC TGGAAAAACG GTTGCAGGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA 
AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG 
CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC 
GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG 
GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC 
TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC 
ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT 
ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC ACCAGAAGCC 
ATGCGGATCT GGATGGACGT TATAGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139640.1
CDS	38..4060
Translation	MAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEGYIIVNEINESCGSSTNLPPSTQFPADAAAAATGPTTAVLMSQPQSKEQRSSVCPGFMFPSTLSSHPITQPPSAPWPEAVATSVDSFPLNDTPPPLPAKKHRRQQQQDQQHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGKSKKGRMNFSAGAVSNSKSSSVKGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283855.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283855.1

ORF Insert Sequence:

ATGGCAGAGC AGAGCCACAT GCAGAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
GGCTTTGTGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA 
AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACAAAGCAGT TCTCTTGCGA TGGAAGTGAC 
AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA 
TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG 
AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT 
GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG 
AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCGCCCATCA ACAGGTCAGG GGCGGCCAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG 
CCCAAGTTGA GCAGACACCG GGACCCGGCA TCTGAAAACA TCAGTTTAAG AACCAGGAAG 
TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT 
AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT 
TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATA GCATTATAGA CAATGACAAT 
TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG 
GGCAGTCCGT ACGTGAGTTC CACCCTCAGT GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG 
GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCGTT TGCAGCCAGA 
AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC 
GGACGAGACG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG 
GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC 
ACAAAGCCTG ACAGTCGCGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT 
AAAGAGCTGG AGAAACTGCA GCTCTCCGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG 
AGCCCGGACA CCAGGTATAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA 
GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CTCCAGGAAT 
GATGAACTGC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA 
GCTTCCGGCG AGTCTGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC 
CTTGAAGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA 
GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT 
ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA 
CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG 
GAGAACCTGA CCCAGCAGCT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA 
CGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA 
AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAGAT TAATGAGTCA 
TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTTC CTGCTGATGC TGCTGCTGCT 
GCCACTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG 
TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT 
CCATCAGCCC CTTGGCCTGA AGCTGTGGCT ACCTCTGTCG ATTCTTTTCC TTTAAATGAC 
ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT 
TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT 
TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG 
AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG 
CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGG 
AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGTTCA 
AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA 
TTTTGCAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT 
CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG 
ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC 
CGAATAGAAG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC CAGGCTGCTT 
GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT 
CGAGAACTCC TGGAAAAACG GTTGCAGGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA 
AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG 
CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC 
GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG 
GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC 
TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC 
ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT 
ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC ACCAGAAGCC 
ATGCGGATCT GGATGGACGT TATAGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283866.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139651.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBu42736
Clone ID Related Accession (Same CDS sequence)	XM_025283861.1 , XM_025283839.1 , XM_025283846.1 , XM_025283855.1 , XM_025283866.1 , XM_025283868.1
Accession Version	XM_025283866.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AGAGCCACAT GCAGAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
GGCTTTGTGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA 
AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACAAAGCAGT TCTCTTGCGA TGGAAGTGAC 
AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA 
TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG 
AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT 
GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG 
AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCGCCCATCA ACAGGTCAGG GGCGGCCAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG 
CCCAAGTTGA GCAGACACCG GGACCCGGCA TCTGAAAACA TCAGTTTAAG AACCAGGAAG 
TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT 
AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT 
TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATA GCATTATAGA CAATGACAAT 
TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG 
GGCAGTCCGT ACGTGAGTTC CACCCTCAGT GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG 
GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCGTT TGCAGCCAGA 
AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC 
GGACGAGACG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG 
GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC 
ACAAAGCCTG ACAGTCGCGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT 
AAAGAGCTGG AGAAACTGCA GCTCTCCGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG 
AGCCCGGACA CCAGGTATAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA 
GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CTCCAGGAAT 
GATGAACTGC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA 
GCTTCCGGCG AGTCTGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC 
CTTGAAGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA 
GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT 
ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA 
CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG 
GAGAACCTGA CCCAGCAGCT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA 
CGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA 
AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAGAT TAATGAGTCA 
TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTTC CTGCTGATGC TGCTGCTGCT 
GCCACTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG 
TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT 
CCATCAGCCC CTTGGCCTGA AGCTGTGGCT ACCTCTGTCG ATTCTTTTCC TTTAAATGAC 
ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT 
TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT 
TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG 
AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG 
CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGG 
AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGTTCA 
AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA 
TTTTGCAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT 
CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG 
ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC 
CGAATAGAAG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC CAGGCTGCTT 
GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT 
CGAGAACTCC TGGAAAAACG GTTGCAGGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA 
AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG 
CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC 
GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG 
GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC 
TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC 
ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT 
ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC ACCAGAAGCC 
ATGCGGATCT GGATGGACGT TATAGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139651.1
CDS	334..4356
Translation	MAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEGYIIVNEINESCGSSTNLPPSTQFPADAAAAATGPTTAVLMSQPQSKEQRSSVCPGFMFPSTLSSHPITQPPSAPWPEAVATSVDSFPLNDTPPPLPAKKHRRQQQQDQQHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGKSKKGRMNFSAGAVSNSKSSSVKGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283866.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283866.1

ORF Insert Sequence:

ATGGCAGAGC AGAGCCACAT GCAGAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
GGCTTTGTGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA 
AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACAAAGCAGT TCTCTTGCGA TGGAAGTGAC 
AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA 
TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG 
AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT 
GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG 
AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCGCCCATCA ACAGGTCAGG GGCGGCCAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG 
CCCAAGTTGA GCAGACACCG GGACCCGGCA TCTGAAAACA TCAGTTTAAG AACCAGGAAG 
TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT 
AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT 
TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATA GCATTATAGA CAATGACAAT 
TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG 
GGCAGTCCGT ACGTGAGTTC CACCCTCAGT GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG 
GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCGTT TGCAGCCAGA 
AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC 
GGACGAGACG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG 
GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC 
ACAAAGCCTG ACAGTCGCGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT 
AAAGAGCTGG AGAAACTGCA GCTCTCCGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG 
AGCCCGGACA CCAGGTATAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA 
GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CTCCAGGAAT 
GATGAACTGC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA 
GCTTCCGGCG AGTCTGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC 
CTTGAAGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA 
GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT 
ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA 
CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG 
GAGAACCTGA CCCAGCAGCT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA 
CGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA 
AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAGAT TAATGAGTCA 
TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTTC CTGCTGATGC TGCTGCTGCT 
GCCACTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG 
TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT 
CCATCAGCCC CTTGGCCTGA AGCTGTGGCT ACCTCTGTCG ATTCTTTTCC TTTAAATGAC 
ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT 
TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT 
TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG 
AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG 
CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGG 
AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGTTCA 
AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA 
TTTTGCAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT 
CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG 
ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC 
CGAATAGAAG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC CAGGCTGCTT 
GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT 
CGAGAACTCC TGGAAAAACG GTTGCAGGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA 
AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG 
CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC 
GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG 
GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC 
TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC 
ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT 
ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC ACCAGAAGCC 
ATGCGGATCT GGATGGACGT TATAGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283868.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139653.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBu42736
Clone ID Related Accession (Same CDS sequence)	XM_025283861.1 , XM_025283839.1 , XM_025283846.1 , XM_025283855.1 , XM_025283866.1 , XM_025283868.1
Accession Version	XM_025283868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AGAGCCACAT GCAGAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
GGCTTTGTGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA 
AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACAAAGCAGT TCTCTTGCGA TGGAAGTGAC 
AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA 
TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG 
AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT 
GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG 
AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCGCCCATCA ACAGGTCAGG GGCGGCCAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG 
CCCAAGTTGA GCAGACACCG GGACCCGGCA TCTGAAAACA TCAGTTTAAG AACCAGGAAG 
TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT 
AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT 
TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATA GCATTATAGA CAATGACAAT 
TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG 
GGCAGTCCGT ACGTGAGTTC CACCCTCAGT GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG 
GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCGTT TGCAGCCAGA 
AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC 
GGACGAGACG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG 
GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC 
ACAAAGCCTG ACAGTCGCGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT 
AAAGAGCTGG AGAAACTGCA GCTCTCCGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG 
AGCCCGGACA CCAGGTATAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA 
GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CTCCAGGAAT 
GATGAACTGC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA 
GCTTCCGGCG AGTCTGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC 
CTTGAAGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA 
GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT 
ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA 
CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG 
GAGAACCTGA CCCAGCAGCT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA 
CGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA 
AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAGAT TAATGAGTCA 
TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTTC CTGCTGATGC TGCTGCTGCT 
GCCACTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG 
TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT 
CCATCAGCCC CTTGGCCTGA AGCTGTGGCT ACCTCTGTCG ATTCTTTTCC TTTAAATGAC 
ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT 
TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT 
TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG 
AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG 
CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGG 
AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGTTCA 
AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA 
TTTTGCAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT 
CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG 
ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC 
CGAATAGAAG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC CAGGCTGCTT 
GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT 
CGAGAACTCC TGGAAAAACG GTTGCAGGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA 
AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG 
CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC 
GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG 
GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC 
TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC 
ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT 
ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC ACCAGAAGCC 
ATGCGGATCT GGATGGACGT TATAGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139653.1
CDS	423..4445
Translation	MAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEGYIIVNEINESCGSSTNLPPSTQFPADAAAAATGPTTAVLMSQPQSKEQRSSVCPGFMFPSTLSSHPITQPPSAPWPEAVATSVDSFPLNDTPPPLPAKKHRRQQQQDQQHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGKSKKGRMNFSAGAVSNSKSSSVKGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283868.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283868.1

ORF Insert Sequence:

ATGGCAGAGC AGAGCCACAT GCAGAAGCAG CTGGATTTAC AGAATGGTAG CTTAGAGGCA 
GGCTTTGTGG AGAACTCCCT CGAAAACGAT TCCCAAAACA TGATGGAGAG CCTGAGCCCA 
AAGAAATATT CTTCCAGTCT GAGATTTAAA GCCAATGGAG ACTGTTCTGG CTCCTATTTA 
ACCCTCTCAC AACCTGTGCC TGCTAAAAGA AGCCCTTCTC CTTTGGGAAC CAGTGTCAGA 
AGTAGCCCTT CTTTGGCCAA AATTCAGGGA ACAAAGCAGT TCTCTTGCGA TGGAAGTGAC 
AAAAGTATTT CCATGAAACC GCCCACCCCT TTACGCAGCA CCTCACCCTC CCTTAGTGGA 
TATGCACTTG GGAGAGCAGA CTTTGATCAT TTTCTTGGCC GGGACGCCGA AAGGTCCTTG 
AGGCTTTCAG AGAAGCCTCC CTATTCCAAA TATAGCTCAA GGAATAAATC CCATGACAAT 
GTCTACTTTC TCGGAGGGCT GGAAGGCCGA AAAGCATCTG GCTCGCTCCT GACCATGTGG 
AATGGAAGTT CCCTGAGTGA CACTGGCTCC TCGCCCATCA ACAGGTCAGG GGCGGCCAGC 
ATGCCTTCAA GCCCAAAGCA AGCCAGGAAA ATAAGCATCC AGGACAACCT GACACTTCAG 
CCCAAGTTGA GCAGACACCG GGACCCGGCA TCTGAAAACA TCAGTTTAAG AACCAGGAAG 
TACTCTAGCG GCAGCCTGAG TCACATGGGC GCCTACAGCC GCTCTCTCCC CAGGTTGCAT 
AGAGCCACCG AGAACCAGTT GGCACCCCTC AGTCTGCCTC CAAGAAGCTC TCTGGGCAAT 
TCCAAACGAA CGAAACTGGG GGAAAAGGAT CTACCTCATA GCATTATAGA CAATGACAAT 
TACCTGAATT TTTCTTCTTT GAGCTCAGGA GCTTTGCCCT ATAAAGCCTC TGCCTCCGAG 
GGCAGTCCGT ACGTGAGTTC CACCCTCAGT GTGCCTGCCA GTCCTCGAGT GGCTCGCAAG 
ATGCTTCTGG CCTCCACCTC CTCCTGCACC TCTGACGACC TTGACAGGGC TTCCTACTCG 
GGTACCAGCC CTGGTCATTC GTTTCCTCCT GGGGAGCTGG ACCGAGCGTT TGCAGCCAGA 
AGGAACTTCT CGTGTGGATC TGTCGAGTTT GATGATGCAG ACTTGGACAG CCTCAGACAG 
GCCTCAGGAA CCCCCCAGCC TGTCCTGCGG GAACGGAAAA GCAGCATTAG CTCCATTTCC 
GGACGAGACG ACCTGATGGA TTATCACCGG CGGCAGAGGG AGGAGAGACT CAGGGAGCAG 
GAGATGGAGC GATTGGAGAG ACAGCGTCTG GAGACCATCC TCAGCCTCTG TGCTGAGTAC 
ACAAAGCCTG ACAGTCGCGT GTCCCCCGGC ACCACCGTGG CAGATGTGCA GAAAATCAAT 
AAAGAGCTGG AGAAACTGCA GCTCTCCGAT GAGGAGTCTG TGTTTGAAGA AGCCCTGGTG 
AGCCCGGACA CCAGGTATAG GTGCCACCAG AAAAGCGCTC TCCACGACGC GGACCTGGCA 
GGCTTTGGGA GCCTCAGTCA GAGCAGTGCC AGCTTCCTCC CGCCACGGGG CTCCAGGAAT 
GATGAACTGC TCGGGGACCT CGCCAGGACT CCCCCGCCGC CCTCCTCTGC CTTTCTGAAA 
GCTTCCGGCG AGTCTGCTTA CCTAAGTATC CTACCAAAGA CCCCAGAGGG TATAAGTGAA 
GAACAAAGGA TTCAGGAGTT GACTGCGATG GAAGAGACCC GGATAGCAAT TCTGAACAAC 
CTTGAAGAAC TTGAACAAAA AATCAAAGAT ATAAATGACC AGATGGAGGA ATCTTCCAGA 
GAGCTGGATA TGGAATGTGC TCTCTTGGAT GGAGAACAAA AATCAGAAAC AACTGAACTT 
ATGAAAGAGA AGGAGATTTT GGATCATCTA AACCGGAAAA TCACAGAACT GGAAAAGAAC 
ATTGTTGGTG AAAAGACCAA GGAGAAGGTA AAGCTTGATG CTGAAAGGGA AAAACTAGAG 
AGGCTTCAGG AGCTTTACTC CGAGCAGAAG ACCCAGCTGG ACAATTGTCC TGAGTCCATG 
AGGGAACAGT TACAACAACA ACTGAAGAGG GATGCCGACC TACTGGATGT TGAGAGCAAA 
CACTTTGAAG ACCTGGAATT CCAGCAGCTT GAGCGTGAAA GCCGTCTAGA TGAGGAAAAG 
GAGAACCTGA CCCAGCAGCT CCTGCGTGAA GTGGCTGAGT ATCAACGGAA CATTGTTACT 
AGAAAGGAAA AAATTTCTGC ATTGAAAAAG CAAGCTAATC ACATTGTCCA GCAGGCTCAA 
CGAGAACAAG ATCATTTTGT GAAAGAAAAG AATAATTTAA TAATGATGCT GCAGAGAGAA 
AAGGAAAATC TTTGTAATTT GGAAAAGAAA TACTCCAGCC TCTCTGGGGG GAAAGGGTTT 
CCTGTCACCC CCAATACTCT GAAAGAGGGC TATATCATTG TAAATGAGAT TAATGAGTCA 
TGTGGCAGTT CCACGAATCT CCCCCCTTCC ACTCAGTTTC CTGCTGATGC TGCTGCTGCT 
GCCACTGGGC CTACCACAGC TGTGCTGATG AGCCAGCCAC AAAGTAAAGA GCAAAGATCG 
TCTGTCTGTC CTGGATTTAT GTTTCCTTCC ACTCTTTCTT CTCATCCTAT CACTCAACCT 
CCATCAGCCC CTTGGCCTGA AGCTGTGGCT ACCTCTGTCG ATTCTTTTCC TTTAAATGAC 
ACACCTCCTC CTCTACCAGC TAAGAAACAC AGGAGGCAAC AGCAGCAGGA CCAGCAGCAT 
TTTAGAAGTC TGGAAGAAAG GAAAAAACAG CACCAGGAAG GCCTCTATCT GAGTGACACT 
TTGCCTCGAA AGAAAACCAC ACCTTCCATA TCCCCACATT TCAGCAGTGC TACTATGGGG 
AGAAGCACCA CCCCAAAGGG CCACCTGCCC CTGGGACAGA GCAACAGCTG TGGCAGCGTG 
CTCCCCCACT CGCTAGCAAC CATGACCAAA GACTCAGAAT CTCGGAGGAT GCTCAGAGGG 
AAGTCCAAGA AAGGAAGAAT GAATTTCTCA GCAGGAGCAG TTAGTAATTC CAAAAGTTCA 
AGTGTCAAAG GATATAATCA CCAACAGATG AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA 
TTTTGCAACC GCACAGCATC TGAATCGAAT GTCTACTTGA ACAGTTTCCA CTACCCAGAT 
CACAGCTACA AGGACCAGGC CTTTGATACT CTGAGCCTAG ACAGCTCTGA CAGCATGGAG 
ACCAGCATCT CTGCCTGCTC ACCTGACAAT ATCTCTAGTG CCAGCACTTC AAATATTGCC 
CGAATAGAAG AAATGGAGAG ACTGTTGAAG CAGGCTCATG CAGAGAAGAC CAGGCTGCTT 
GAATCCAGGG AACGAGAAAT GGAAGCCAAG AAACGCGCTC TGGAAGAAGA AAAACGACGT 
CGAGAACTCC TGGAAAAACG GTTGCAGGAA GAAACTAGCC AGAGGCAGAA GTTAATTGAA 
AAGGAAGTAA AAATAAGGGA GAAACAAAGG GCACAGGCTC GACCTCTGAC CCGCTATCTG 
CCTGTGCGGA AGGAAGACTT TGATTTGCGG AGCCACGTAG AGACTGCTGG CCACAATATC 
GATACGTGTT ATCATGTGTC AATCACAGAG AAGACCTGCA GAGGATTCCT CATCAAAATG 
GGTGGAAAAA TTAAAACCTG GAAAAAACGT TGGTTTGTTT TTGATCGGAA CAAGCGCACC 
TTCTCTTATT ATGCAGACAA GCATGAAGCT AAATTAAAAG GAGTAATATA CTTTCAAGCC 
ATTGAAGAAG TATATTATGA TCACCTCAAG AACGCTAATA AGAGTCCTAA TCCTTTACTT 
ACCTTTAGCG TCAAGACACA TGACAGAATC TACTACATGG TGGCCCCGTC ACCAGAAGCC 
ATGCGGATCT GGATGGACGT TATAGTGACT GGGGCAGAAG GCTACACTCA CTTCTTGTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283874.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139659.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X11, mRNA.

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CloneID	OBu42737
Clone ID Related Accession (Same CDS sequence)	XM_025283874.1
Accession Version	XM_025283874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGATGCCGA CCTACTGGAT 
GTTGAGAGCA AACACTTTGA AGACCTGGAA TTCCAGCAGC TTGAGCGTGA AAGCCGTCTA 
GATGAGGAAA AGGAGAACCT GACCCAGCAG CTCCTGCGTG AAGTGGCTGA GTATCAACGG 
AACATTGTTA CTAGAAAGGA AAAAATTTCT GCATTGAAAA AGCAAGCTAA TCACATTGTC 
CAGCAGGCTC AACGAGAACA AGATCATTTT GTGAAAGAAA AGAATAATTT AATAATGATG 
CTGCAGAGAG AAAAGGAAAA TCTTTGTAAT TTGGAAAAGA AATACTCCAG CCTCTCTGGG 
GGGAAAGGGT TTCCTGTCAC CCCCAATACT CTGAAAGAGG GCTATATCAT TGTAAATGAG 
ATTAATGAGT CATGTGGCAG TTCCACGAAT CTCCCCCCTT CCACTCAGTT TCCTGCTGAT 
GCTGCTGCTG CTGCCACTGG GCCTACCACA GCTGTGCTGA TGAGCCAGCC ACAAAGTAAA 
GAGCAAAGAT CGTCTGTCTG TCCTGGATTT ATGTTTCCTT CCACTCTTTC TTCTCATCCT 
ATCACTCAAC CTCCATCAGC CCCTTGGCCT GAAGCTGTGG CTACCTCTGT CGATTCTTTT 
CCTTTAAATG ACACACCTCC TCCTCTACCA GCTAAGAAAC ACAGGAGGCA ACAGCAGCAG 
GACCAGCAGC ATTTTAGAAG TCTGGAAGAA AGGAAAAAAC AGCACCAGGA AGGCCTCTAT 
CTGAGTGACA CTTTGCCTCG AAAGAAAACC ACACCTTCCA TATCCCCACA TTTCAGCAGT 
GCTACTATGG GGAGAAGCAC CACCCCAAAG GGCCACCTGC CCCTGGGACA GAGCAACAGC 
TGTGGCAGCG TGCTCCCCCA CTCGCTAGCA ACCATGACCA AAGACTCAGA ATCTCGGAGG 
ATGCTCAGAG GGAAGTCCAA GAAAGGAAGA ATGAATTTCT CAGCAGGAGC AGTTAGTAAT 
TCCAAAAGTT CAAGTGTCAA AGGATATAAT CACCAACAGA TGAGTGAAGG ACAAAGGCAG 
AAGTCTTCTG AATTTTGCAA CCGCACAGCA TCTGAATCGA ATGTCTACTT GAACAGTTTC 
CACTACCCAG ATCACAGCTA CAAGGACCAG GCCTTTGATA CTCTGAGCCT AGACAGCTCT 
GACAGCATGG AGACCAGCAT CTCTGCCTGC TCACCTGACA ATATCTCTAG TGCCAGCACT 
TCAAATATTG CCCGAATAGA AGAAATGGAG AGACTGTTGA AGCAGGCTCA TGCAGAGAAG 
ACCAGGCTGC TTGAATCCAG GGAACGAGAA ATGGAAGCCA AGAAACGCGC TCTGGAAGAA 
GAAAAACGAC GTCGAGAACT CCTGGAAAAA CGGTTGCAGG AAGAAACTAG CCAGAGGCAG 
AAGTTAATTG AAAAGGAAGT AAAAATAAGG GAGAAACAAA GGGCACAGGC TCGACCTCTG 
ACCCGCTATC TGCCTGTGCG GAAGGAAGAC TTTGATTTGC GGAGCCACGT AGAGACTGCT 
GGCCACAATA TCGATACGTG TTATCATGTG TCAATCACAG AGAAGACCTG CAGAGGATTC 
CTCATCAAAA TGGGTGGAAA AATTAAAACC TGGAAAAAAC GTTGGTTTGT TTTTGATCGG 
AACAAGCGCA CCTTCTCTTA TTATGCAGAC AAGCATGAAG CTAAATTAAA AGGAGTAATA 
TACTTTCAAG CCATTGAAGA AGTATATTAT GATCACCTCA AGAACGCTAA TAAGAGTCCT 
AATCCTTTAC TTACCTTTAG CGTCAAGACA CATGACAGAA TCTACTACAT GGTGGCCCCG 
TCACCAGAAG CCATGCGGAT CTGGATGGAC GTTATAGTGA CTGGGGCAGA AGGCTACACT 
CACTTCTTGT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139659.1
CDS	373..4347
Translation	MEEDNTQRKDTKEEGVGDRQPVDSNKIMAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEGYIIVNEINESCGSSTNLPPSTQFPADAAAAATGPTTAVLMSQPQSKEQRSSVCPGFMFPSTLSSHPITQPPSAPWPEAVATSVDSFPLNDTPPPLPAKKHRRQQQQDQQHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGKSKKGRMNFSAGAVSNSKSSSVKGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283874.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283874.1

ORF Insert Sequence:

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGATGCCGA CCTACTGGAT 
GTTGAGAGCA AACACTTTGA AGACCTGGAA TTCCAGCAGC TTGAGCGTGA AAGCCGTCTA 
GATGAGGAAA AGGAGAACCT GACCCAGCAG CTCCTGCGTG AAGTGGCTGA GTATCAACGG 
AACATTGTTA CTAGAAAGGA AAAAATTTCT GCATTGAAAA AGCAAGCTAA TCACATTGTC 
CAGCAGGCTC AACGAGAACA AGATCATTTT GTGAAAGAAA AGAATAATTT AATAATGATG 
CTGCAGAGAG AAAAGGAAAA TCTTTGTAAT TTGGAAAAGA AATACTCCAG CCTCTCTGGG 
GGGAAAGGGT TTCCTGTCAC CCCCAATACT CTGAAAGAGG GCTATATCAT TGTAAATGAG 
ATTAATGAGT CATGTGGCAG TTCCACGAAT CTCCCCCCTT CCACTCAGTT TCCTGCTGAT 
GCTGCTGCTG CTGCCACTGG GCCTACCACA GCTGTGCTGA TGAGCCAGCC ACAAAGTAAA 
GAGCAAAGAT CGTCTGTCTG TCCTGGATTT ATGTTTCCTT CCACTCTTTC TTCTCATCCT 
ATCACTCAAC CTCCATCAGC CCCTTGGCCT GAAGCTGTGG CTACCTCTGT CGATTCTTTT 
CCTTTAAATG ACACACCTCC TCCTCTACCA GCTAAGAAAC ACAGGAGGCA ACAGCAGCAG 
GACCAGCAGC ATTTTAGAAG TCTGGAAGAA AGGAAAAAAC AGCACCAGGA AGGCCTCTAT 
CTGAGTGACA CTTTGCCTCG AAAGAAAACC ACACCTTCCA TATCCCCACA TTTCAGCAGT 
GCTACTATGG GGAGAAGCAC CACCCCAAAG GGCCACCTGC CCCTGGGACA GAGCAACAGC 
TGTGGCAGCG TGCTCCCCCA CTCGCTAGCA ACCATGACCA AAGACTCAGA ATCTCGGAGG 
ATGCTCAGAG GGAAGTCCAA GAAAGGAAGA ATGAATTTCT CAGCAGGAGC AGTTAGTAAT 
TCCAAAAGTT CAAGTGTCAA AGGATATAAT CACCAACAGA TGAGTGAAGG ACAAAGGCAG 
AAGTCTTCTG AATTTTGCAA CCGCACAGCA TCTGAATCGA ATGTCTACTT GAACAGTTTC 
CACTACCCAG ATCACAGCTA CAAGGACCAG GCCTTTGATA CTCTGAGCCT AGACAGCTCT 
GACAGCATGG AGACCAGCAT CTCTGCCTGC TCACCTGACA ATATCTCTAG TGCCAGCACT 
TCAAATATTG CCCGAATAGA AGAAATGGAG AGACTGTTGA AGCAGGCTCA TGCAGAGAAG 
ACCAGGCTGC TTGAATCCAG GGAACGAGAA ATGGAAGCCA AGAAACGCGC TCTGGAAGAA 
GAAAAACGAC GTCGAGAACT CCTGGAAAAA CGGTTGCAGG AAGAAACTAG CCAGAGGCAG 
AAGTTAATTG AAAAGGAAGT AAAAATAAGG GAGAAACAAA GGGCACAGGC TCGACCTCTG 
ACCCGCTATC TGCCTGTGCG GAAGGAAGAC TTTGATTTGC GGAGCCACGT AGAGACTGCT 
GGCCACAATA TCGATACGTG TTATCATGTG TCAATCACAG AGAAGACCTG CAGAGGATTC 
CTCATCAAAA TGGGTGGAAA AATTAAAACC TGGAAAAAAC GTTGGTTTGT TTTTGATCGG 
AACAAGCGCA CCTTCTCTTA TTATGCAGAC AAGCATGAAG CTAAATTAAA AGGAGTAATA 
TACTTTCAAG CCATTGAAGA AGTATATTAT GATCACCTCA AGAACGCTAA TAAGAGTCCT 
AATCCTTTAC TTACCTTTAG CGTCAAGACA CATGACAGAA TCTACTACAT GGTGGCCCCG 
TCACCAGAAG CCATGCGGAT CTGGATGGAC GTTATAGTGA CTGGGGCAGA AGGCTACACT 
CACTTCTTGT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283875.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139660.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X12, mRNA.

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CloneID	OBu42738
Clone ID Related Accession (Same CDS sequence)	XM_025283875.1
Accession Version	XM_025283875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGCA AAGATCGTCT 
GTCTGTCCTG GATTTATGTT TCCTTCCACT CTTTCTTCTC ATCCTATCAC TCAACCTCCA 
TCAGCCCCTT GGCCTGAAGC TGTGGCTACC TCTGTCGATT CTTTTCCTTT AAATGACACA 
CCTCCTCCTC TACCAGCTAA GAAACACAGG AGGCAACAGC AGCAGGACCA GCAGCATTTT 
AGAAGTCTGG AAGAAAGGAA AAAACAGCAC CAGGAAGGCC TCTATCTGAG TGACACTTTG 
CCTCGAAAGA AAACCACACC TTCCATATCC CCACATTTCA GCAGTGCTAC TATGGGGAGA 
AGCACCACCC CAAAGGGCCA CCTGCCCCTG GGACAGAGCA ACAGCTGTGG CAGCGTGCTC 
CCCCACTCGC TAGCAACCAT GACCAAAGAC TCAGAATCTC GGAGGATGCT CAGAGGGAAG 
TCCAAGAAAG GAAGAATGAA TTTCTCAGCA GGAGCAGTTA GTAATTCCAA AAGTTCAAGT 
GTCAAAGGAT ATAATCACCA ACAGATGAGT GAAGGACAAA GGCAGAAGTC TTCTGAATTT 
TGCAACCGCA CAGCATCTGA ATCGAATGTC TACTTGAACA GTTTCCACTA CCCAGATCAC 
AGCTACAAGG ACCAGGCCTT TGATACTCTG AGCCTAGACA GCTCTGACAG CATGGAGACC 
AGCATCTCTG CCTGCTCACC TGACAATATC TCTAGTGCCA GCACTTCAAA TATTGCCCGA 
ATAGAAGAAA TGGAGAGACT GTTGAAGCAG GCTCATGCAG AGAAGACCAG GCTGCTTGAA 
TCCAGGGAAC GAGAAATGGA AGCCAAGAAA CGCGCTCTGG AAGAAGAAAA ACGACGTCGA 
GAACTCCTGG AAAAACGGTT GCAGGAAGAA ACTAGCCAGA GGCAGAAGTT AATTGAAAAG 
GAAGTAAAAA TAAGGGAGAA ACAAAGGGCA CAGGCTCGAC CTCTGACCCG CTATCTGCCT 
GTGCGGAAGG AAGACTTTGA TTTGCGGAGC CACGTAGAGA CTGCTGGCCA CAATATCGAT 
ACGTGTTATC ATGTGTCAAT CACAGAGAAG ACCTGCAGAG GATTCCTCAT CAAAATGGGT 
GGAAAAATTA AAACCTGGAA AAAACGTTGG TTTGTTTTTG ATCGGAACAA GCGCACCTTC 
TCTTATTATG CAGACAAGCA TGAAGCTAAA TTAAAAGGAG TAATATACTT TCAAGCCATT 
GAAGAAGTAT ATTATGATCA CCTCAAGAAC GCTAATAAGA GTCCTAATCC TTTACTTACC 
TTTAGCGTCA AGACACATGA CAGAATCTAC TACATGGTGG CCCCGTCACC AGAAGCCATG 
CGGATCTGGA TGGACGTTAT AGTGACTGGG GCAGAAGGCT ACACTCACTT CTTGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139660.1
CDS	366..4325
Translation	MEEDNTQRKDTKEEGVGDRQPVDSNKIMAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEQRSSVCPGFMFPSTLSSHPITQPPSAPWPEAVATSVDSFPLNDTPPPLPAKKHRRQQQQDQQHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGKSKKGRMNFSAGAVSNSKSSSVKGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283875.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283875.1

ORF Insert Sequence:

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGCA AAGATCGTCT 
GTCTGTCCTG GATTTATGTT TCCTTCCACT CTTTCTTCTC ATCCTATCAC TCAACCTCCA 
TCAGCCCCTT GGCCTGAAGC TGTGGCTACC TCTGTCGATT CTTTTCCTTT AAATGACACA 
CCTCCTCCTC TACCAGCTAA GAAACACAGG AGGCAACAGC AGCAGGACCA GCAGCATTTT 
AGAAGTCTGG AAGAAAGGAA AAAACAGCAC CAGGAAGGCC TCTATCTGAG TGACACTTTG 
CCTCGAAAGA AAACCACACC TTCCATATCC CCACATTTCA GCAGTGCTAC TATGGGGAGA 
AGCACCACCC CAAAGGGCCA CCTGCCCCTG GGACAGAGCA ACAGCTGTGG CAGCGTGCTC 
CCCCACTCGC TAGCAACCAT GACCAAAGAC TCAGAATCTC GGAGGATGCT CAGAGGGAAG 
TCCAAGAAAG GAAGAATGAA TTTCTCAGCA GGAGCAGTTA GTAATTCCAA AAGTTCAAGT 
GTCAAAGGAT ATAATCACCA ACAGATGAGT GAAGGACAAA GGCAGAAGTC TTCTGAATTT 
TGCAACCGCA CAGCATCTGA ATCGAATGTC TACTTGAACA GTTTCCACTA CCCAGATCAC 
AGCTACAAGG ACCAGGCCTT TGATACTCTG AGCCTAGACA GCTCTGACAG CATGGAGACC 
AGCATCTCTG CCTGCTCACC TGACAATATC TCTAGTGCCA GCACTTCAAA TATTGCCCGA 
ATAGAAGAAA TGGAGAGACT GTTGAAGCAG GCTCATGCAG AGAAGACCAG GCTGCTTGAA 
TCCAGGGAAC GAGAAATGGA AGCCAAGAAA CGCGCTCTGG AAGAAGAAAA ACGACGTCGA 
GAACTCCTGG AAAAACGGTT GCAGGAAGAA ACTAGCCAGA GGCAGAAGTT AATTGAAAAG 
GAAGTAAAAA TAAGGGAGAA ACAAAGGGCA CAGGCTCGAC CTCTGACCCG CTATCTGCCT 
GTGCGGAAGG AAGACTTTGA TTTGCGGAGC CACGTAGAGA CTGCTGGCCA CAATATCGAT 
ACGTGTTATC ATGTGTCAAT CACAGAGAAG ACCTGCAGAG GATTCCTCAT CAAAATGGGT 
GGAAAAATTA AAACCTGGAA AAAACGTTGG TTTGTTTTTG ATCGGAACAA GCGCACCTTC 
TCTTATTATG CAGACAAGCA TGAAGCTAAA TTAAAAGGAG TAATATACTT TCAAGCCATT 
GAAGAAGTAT ATTATGATCA CCTCAAGAAC GCTAATAAGA GTCCTAATCC TTTACTTACC 
TTTAGCGTCA AGACACATGA CAGAATCTAC TACATGGTGG CCCCGTCACC AGAAGCCATG 
CGGATCTGGA TGGACGTTAT AGTGACTGGG GCAGAAGGCT ACACTCACTT CTTGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283883.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139668.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBu42739
Clone ID Related Accession (Same CDS sequence)	XM_025283883.1
Accession Version	XM_025283883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT 
GTAAATGAGA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTT 
CCTGCTGATG CTGCTGCTGC TGCCACTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA 
CAAAGTAAAG AGCATTTTAG AAGTCTGGAA GAAAGGAAAA AACAGCACCA GGAAGGCCTC 
TATCTGAGTG ACACTTTGCC TCGAAAGAAA ACCACACCTT CCATATCCCC ACATTTCAGC 
AGTGCTACTA TGGGGAGAAG CACCACCCCA AAGGGCCACC TGCCCCTGGG ACAGAGCAAC 
AGCTGTGGCA GCGTGCTCCC CCACTCGCTA GCAACCATGA CCAAAGACTC AGAATCTCGG 
AGGATGCTCA GAGGGAAGTC CAAGAAAGGA AGAATGAATT TCTCAGCAGG AGCAGTTAGT 
AATTCCAAAA GTTCAAGTGT CAAAGGATAT AATCACCAAC AGATGAGTGA AGGACAAAGG 
CAGAAGTCTT CTGAATTTTG CAACCGCACA GCATCTGAAT CGAATGTCTA CTTGAACAGT 
TTCCACTACC CAGATCACAG CTACAAGGAC CAGGCCTTTG ATACTCTGAG CCTAGACAGC 
TCTGACAGCA TGGAGACCAG CATCTCTGCC TGCTCACCTG ACAATATCTC TAGTGCCAGC 
ACTTCAAATA TTGCCCGAAT AGAAGAAATG GAGAGACTGT TGAAGCAGGC TCATGCAGAG 
AAGACCAGGC TGCTTGAATC CAGGGAACGA GAAATGGAAG CCAAGAAACG CGCTCTGGAA 
GAAGAAAAAC GACGTCGAGA ACTCCTGGAA AAACGGTTGC AGGAAGAAAC TAGCCAGAGG 
CAGAAGTTAA TTGAAAAGGA AGTAAAAATA AGGGAGAAAC AAAGGGCACA GGCTCGACCT 
CTGACCCGCT ATCTGCCTGT GCGGAAGGAA GACTTTGATT TGCGGAGCCA CGTAGAGACT 
GCTGGCCACA ATATCGATAC GTGTTATCAT GTGTCAATCA CAGAGAAGAC CTGCAGAGGA 
TTCCTCATCA AAATGGGTGG AAAAATTAAA ACCTGGAAAA AACGTTGGTT TGTTTTTGAT 
CGGAACAAGC GCACCTTCTC TTATTATGCA GACAAGCATG AAGCTAAATT AAAAGGAGTA 
ATATACTTTC AAGCCATTGA AGAAGTATAT TATGATCACC TCAAGAACGC TAATAAGAGT 
CCTAATCCTT TACTTACCTT TAGCGTCAAG ACACATGACA GAATCTACTA CATGGTGGCC 
CCGTCACCAG AAGCCATGCG GATCTGGATG GACGTTATAG TGACTGGGGC AGAAGGCTAC 
ACTCACTTCT TGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139668.1
CDS	360..4277
Translation	MEEDNTQRKDTKEEGVGDRQPVDSNKIMAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEGYIIVNEINESCGSSTNLPPSTQFPADAAAAATGPTTAVLMSQPQSKEHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGKSKKGRMNFSAGAVSNSKSSSVKGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283883.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283883.1

ORF Insert Sequence:

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT 
GTAAATGAGA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTT 
CCTGCTGATG CTGCTGCTGC TGCCACTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA 
CAAAGTAAAG AGCATTTTAG AAGTCTGGAA GAAAGGAAAA AACAGCACCA GGAAGGCCTC 
TATCTGAGTG ACACTTTGCC TCGAAAGAAA ACCACACCTT CCATATCCCC ACATTTCAGC 
AGTGCTACTA TGGGGAGAAG CACCACCCCA AAGGGCCACC TGCCCCTGGG ACAGAGCAAC 
AGCTGTGGCA GCGTGCTCCC CCACTCGCTA GCAACCATGA CCAAAGACTC AGAATCTCGG 
AGGATGCTCA GAGGGAAGTC CAAGAAAGGA AGAATGAATT TCTCAGCAGG AGCAGTTAGT 
AATTCCAAAA GTTCAAGTGT CAAAGGATAT AATCACCAAC AGATGAGTGA AGGACAAAGG 
CAGAAGTCTT CTGAATTTTG CAACCGCACA GCATCTGAAT CGAATGTCTA CTTGAACAGT 
TTCCACTACC CAGATCACAG CTACAAGGAC CAGGCCTTTG ATACTCTGAG CCTAGACAGC 
TCTGACAGCA TGGAGACCAG CATCTCTGCC TGCTCACCTG ACAATATCTC TAGTGCCAGC 
ACTTCAAATA TTGCCCGAAT AGAAGAAATG GAGAGACTGT TGAAGCAGGC TCATGCAGAG 
AAGACCAGGC TGCTTGAATC CAGGGAACGA GAAATGGAAG CCAAGAAACG CGCTCTGGAA 
GAAGAAAAAC GACGTCGAGA ACTCCTGGAA AAACGGTTGC AGGAAGAAAC TAGCCAGAGG 
CAGAAGTTAA TTGAAAAGGA AGTAAAAATA AGGGAGAAAC AAAGGGCACA GGCTCGACCT 
CTGACCCGCT ATCTGCCTGT GCGGAAGGAA GACTTTGATT TGCGGAGCCA CGTAGAGACT 
GCTGGCCACA ATATCGATAC GTGTTATCAT GTGTCAATCA CAGAGAAGAC CTGCAGAGGA 
TTCCTCATCA AAATGGGTGG AAAAATTAAA ACCTGGAAAA AACGTTGGTT TGTTTTTGAT 
CGGAACAAGC GCACCTTCTC TTATTATGCA GACAAGCATG AAGCTAAATT AAAAGGAGTA 
ATATACTTTC AAGCCATTGA AGAAGTATAT TATGATCACC TCAAGAACGC TAATAAGAGT 
CCTAATCCTT TACTTACCTT TAGCGTCAAG ACACATGACA GAATCTACTA CATGGTGGCC 
CCGTCACCAG AAGCCATGCG GATCTGGATG GACGTTATAG TGACTGGGGC AGAAGGCTAC 
ACTCACTTCT TGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283891.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139676.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X14, mRNA.

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CloneID	OBu42740
Clone ID Related Accession (Same CDS sequence)	XM_025283891.1
Accession Version	XM_025283891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT 
GTAAATGAGA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTT 
CCTGCTGATG CTGCTGCTGC TGCCACTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA 
CAAAGTAAAG AGCATTTTAG AAGTCTGGAA GAAAGGAAAA AACAGCACCA GGAAGGCCTC 
TATCTGAGTG ACACTTTGCC TCGAAAGAAA ACCACACCTT CCATATCCCC ACATTTCAGC 
AGTGCTACTA TGGGGAGAAG CACCACCCCA AAGGGCCACC TGCCCCTGGG ACAGAGCAAC 
AGCTGTGGCA GCGTGCTCCC CCACTCGCTA GCAACCATGA CCAAAGACTC AGAATCTCGG 
AGGATGCTCA GAGGATATAA TCACCAACAG ATGAGTGAAG GACAAAGGCA GAAGTCTTCT 
GAATTTTGCA ACCGCACAGC ATCTGAATCG AATGTCTACT TGAACAGTTT CCACTACCCA 
GATCACAGCT ACAAGGACCA GGCCTTTGAT ACTCTGAGCC TAGACAGCTC TGACAGCATG 
GAGACCAGCA TCTCTGCCTG CTCACCTGAC AATATCTCTA GTGCCAGCAC TTCAAATATT 
GCCCGAATAG AAGAAATGGA GAGACTGTTG AAGCAGGCTC ATGCAGAGAA GACCAGGCTG 
CTTGAATCCA GGGAACGAGA AATGGAAGCC AAGAAACGCG CTCTGGAAGA AGAAAAACGA 
CGTCGAGAAC TCCTGGAAAA ACGGTTGCAG GAAGAAACTA GCCAGAGGCA GAAGTTAATT 
GAAAAGGAAG TAAAAATAAG GGAGAAACAA AGGGCACAGG CTCGACCTCT GACCCGCTAT 
CTGCCTGTGC GGAAGGAAGA CTTTGATTTG CGGAGCCACG TAGAGACTGC TGGCCACAAT 
ATCGATACGT GTTATCATGT GTCAATCACA GAGAAGACCT GCAGAGGATT CCTCATCAAA 
ATGGGTGGAA AAATTAAAAC CTGGAAAAAA CGTTGGTTTG TTTTTGATCG GAACAAGCGC 
ACCTTCTCTT ATTATGCAGA CAAGCATGAA GCTAAATTAA AAGGAGTAAT ATACTTTCAA 
GCCATTGAAG AAGTATATTA TGATCACCTC AAGAACGCTA ATAAGAGTCC TAATCCTTTA 
CTTACCTTTA GCGTCAAGAC ACATGACAGA ATCTACTACA TGGTGGCCCC GTCACCAGAA 
GCCATGCGGA TCTGGATGGA CGTTATAGTG ACTGGGGCAG AAGGCTACAC TCACTTCTTG 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139676.1
CDS	355..4200
Translation	MEEDNTQRKDTKEEGVGDRQPVDSNKIMAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEGYIIVNEINESCGSSTNLPPSTQFPADAAAAATGPTTAVLMSQPQSKEHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283891.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283891.1

ORF Insert Sequence:

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT 
GTAAATGAGA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTT 
CCTGCTGATG CTGCTGCTGC TGCCACTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA 
CAAAGTAAAG AGCATTTTAG AAGTCTGGAA GAAAGGAAAA AACAGCACCA GGAAGGCCTC 
TATCTGAGTG ACACTTTGCC TCGAAAGAAA ACCACACCTT CCATATCCCC ACATTTCAGC 
AGTGCTACTA TGGGGAGAAG CACCACCCCA AAGGGCCACC TGCCCCTGGG ACAGAGCAAC 
AGCTGTGGCA GCGTGCTCCC CCACTCGCTA GCAACCATGA CCAAAGACTC AGAATCTCGG 
AGGATGCTCA GAGGATATAA TCACCAACAG ATGAGTGAAG GACAAAGGCA GAAGTCTTCT 
GAATTTTGCA ACCGCACAGC ATCTGAATCG AATGTCTACT TGAACAGTTT CCACTACCCA 
GATCACAGCT ACAAGGACCA GGCCTTTGAT ACTCTGAGCC TAGACAGCTC TGACAGCATG 
GAGACCAGCA TCTCTGCCTG CTCACCTGAC AATATCTCTA GTGCCAGCAC TTCAAATATT 
GCCCGAATAG AAGAAATGGA GAGACTGTTG AAGCAGGCTC ATGCAGAGAA GACCAGGCTG 
CTTGAATCCA GGGAACGAGA AATGGAAGCC AAGAAACGCG CTCTGGAAGA AGAAAAACGA 
CGTCGAGAAC TCCTGGAAAA ACGGTTGCAG GAAGAAACTA GCCAGAGGCA GAAGTTAATT 
GAAAAGGAAG TAAAAATAAG GGAGAAACAA AGGGCACAGG CTCGACCTCT GACCCGCTAT 
CTGCCTGTGC GGAAGGAAGA CTTTGATTTG CGGAGCCACG TAGAGACTGC TGGCCACAAT 
ATCGATACGT GTTATCATGT GTCAATCACA GAGAAGACCT GCAGAGGATT CCTCATCAAA 
ATGGGTGGAA AAATTAAAAC CTGGAAAAAA CGTTGGTTTG TTTTTGATCG GAACAAGCGC 
ACCTTCTCTT ATTATGCAGA CAAGCATGAA GCTAAATTAA AAGGAGTAAT ATACTTTCAA 
GCCATTGAAG AAGTATATTA TGATCACCTC AAGAACGCTA ATAAGAGTCC TAATCCTTTA 
CTTACCTTTA GCGTCAAGAC ACATGACAGA ATCTACTACA TGGTGGCCCC GTCACCAGAA 
GCCATGCGGA TCTGGATGGA CGTTATAGTG ACTGGGGCAG AAGGCTACAC TCACTTCTTG 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283897.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139682.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBu42741
Clone ID Related Accession (Same CDS sequence)	XM_025283897.1
Accession Version	XM_025283897.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT 
GTAAATGAGA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTT 
CCTGCTGATG CTGCTGCTGC TGCCACTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA 
CAAAGTAAAG AGGGCCACCT GCCCCTGGGA CAGAGCAACA GCTGTGGCAG CGTGCTCCCC 
CACTCGCTAG CAACCATGAC CAAAGACTCA GAATCTCGGA GGATGCTCAG AGGGAAGTCC 
AAGAAAGGAA GAATGAATTT CTCAGCAGGA GCAGTTAGTA ATTCCAAAAG TTCAAGTGTC 
AAAGGATATA ATCACCAACA GATGAGTGAA GGACAAAGGC AGAAGTCTTC TGAATTTTGC 
AACCGCACAG CATCTGAATC GAATGTCTAC TTGAACAGTT TCCACTACCC AGATCACAGC 
TACAAGGACC AGGCCTTTGA TACTCTGAGC CTAGACAGCT CTGACAGCAT GGAGACCAGC 
ATCTCTGCCT GCTCACCTGA CAATATCTCT AGTGCCAGCA CTTCAAATAT TGCCCGAATA 
GAAGAAATGG AGAGACTGTT GAAGCAGGCT CATGCAGAGA AGACCAGGCT GCTTGAATCC 
AGGGAACGAG AAATGGAAGC CAAGAAACGC GCTCTGGAAG AAGAAAAACG ACGTCGAGAA 
CTCCTGGAAA AACGGTTGCA GGAAGAAACT AGCCAGAGGC AGAAGTTAAT TGAAAAGGAA 
GTAAAAATAA GGGAGAAACA AAGGGCACAG GCTCGACCTC TGACCCGCTA TCTGCCTGTG 
CGGAAGGAAG ACTTTGATTT GCGGAGCCAC GTAGAGACTG CTGGCCACAA TATCGATACG 
TGTTATCATG TGTCAATCAC AGAGAAGACC TGCAGAGGAT TCCTCATCAA AATGGGTGGA 
AAAATTAAAA CCTGGAAAAA ACGTTGGTTT GTTTTTGATC GGAACAAGCG CACCTTCTCT 
TATTATGCAG ACAAGCATGA AGCTAAATTA AAAGGAGTAA TATACTTTCA AGCCATTGAA 
GAAGTATATT ATGATCACCT CAAGAACGCT AATAAGAGTC CTAATCCTTT ACTTACCTTT 
AGCGTCAAGA CACATGACAG AATCTACTAC ATGGTGGCCC CGTCACCAGA AGCCATGCGG 
ATCTGGATGG ACGTTATAGT GACTGGGGCA GAAGGCTACA CTCACTTCTT GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139682.1
CDS	362..4138
Translation	MEEDNTQRKDTKEEGVGDRQPVDSNKIMAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEGYIIVNEINESCGSSTNLPPSTQFPADAAAAATGPTTAVLMSQPQSKEGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGKSKKGRMNFSAGAVSNSKSSSVKGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283897.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283897.1

ORF Insert Sequence:

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT 
GTAAATGAGA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTT 
CCTGCTGATG CTGCTGCTGC TGCCACTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA 
CAAAGTAAAG AGGGCCACCT GCCCCTGGGA CAGAGCAACA GCTGTGGCAG CGTGCTCCCC 
CACTCGCTAG CAACCATGAC CAAAGACTCA GAATCTCGGA GGATGCTCAG AGGGAAGTCC 
AAGAAAGGAA GAATGAATTT CTCAGCAGGA GCAGTTAGTA ATTCCAAAAG TTCAAGTGTC 
AAAGGATATA ATCACCAACA GATGAGTGAA GGACAAAGGC AGAAGTCTTC TGAATTTTGC 
AACCGCACAG CATCTGAATC GAATGTCTAC TTGAACAGTT TCCACTACCC AGATCACAGC 
TACAAGGACC AGGCCTTTGA TACTCTGAGC CTAGACAGCT CTGACAGCAT GGAGACCAGC 
ATCTCTGCCT GCTCACCTGA CAATATCTCT AGTGCCAGCA CTTCAAATAT TGCCCGAATA 
GAAGAAATGG AGAGACTGTT GAAGCAGGCT CATGCAGAGA AGACCAGGCT GCTTGAATCC 
AGGGAACGAG AAATGGAAGC CAAGAAACGC GCTCTGGAAG AAGAAAAACG ACGTCGAGAA 
CTCCTGGAAA AACGGTTGCA GGAAGAAACT AGCCAGAGGC AGAAGTTAAT TGAAAAGGAA 
GTAAAAATAA GGGAGAAACA AAGGGCACAG GCTCGACCTC TGACCCGCTA TCTGCCTGTG 
CGGAAGGAAG ACTTTGATTT GCGGAGCCAC GTAGAGACTG CTGGCCACAA TATCGATACG 
TGTTATCATG TGTCAATCAC AGAGAAGACC TGCAGAGGAT TCCTCATCAA AATGGGTGGA 
AAAATTAAAA CCTGGAAAAA ACGTTGGTTT GTTTTTGATC GGAACAAGCG CACCTTCTCT 
TATTATGCAG ACAAGCATGA AGCTAAATTA AAAGGAGTAA TATACTTTCA AGCCATTGAA 
GAAGTATATT ATGATCACCT CAAGAACGCT AATAAGAGTC CTAATCCTTT ACTTACCTTT 
AGCGTCAAGA CACATGACAG AATCTACTAC ATGGTGGCCC CGTCACCAGA AGCCATGCGG 
ATCTGGATGG ACGTTATAGT GACTGGGGCA GAAGGCTACA CTCACTTCTT GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283901.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139686.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBu42742
Clone ID Related Accession (Same CDS sequence)	XM_025283901.1
Accession Version	XM_025283901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGCA TTTTAGAAGT 
CTGGAAGAAA GGAAAAAACA GCACCAGGAA GGCCTCTATC TGAGTGACAC TTTGCCTCGA 
AAGAAAACCA CACCTTCCAT ATCCCCACAT TTCAGCAGTG CTACTATGGG GAGAAGCACC 
ACCCCAAAGG GCCACCTGCC CCTGGGACAG AGCAACAGCT GTGGCAGCGT GCTCCCCCAC 
TCGCTAGCAA CCATGACCAA AGACTCAGAA TCTCGGAGGA TGCTCAGAGG GAAGTCCAAG 
AAAGGAAGAA TGAATTTCTC AGCAGGAGCA GTTAGTAATT CCAAAAGTTC AAGTGTCAAA 
GGATATAATC ACCAACAGAT GAGTGAAGGA CAAAGGCAGA AGTCTTCTGA ATTTTGCAAC 
CGCACAGCAT CTGAATCGAA TGTCTACTTG AACAGTTTCC ACTACCCAGA TCACAGCTAC 
AAGGACCAGG CCTTTGATAC TCTGAGCCTA GACAGCTCTG ACAGCATGGA GACCAGCATC 
TCTGCCTGCT CACCTGACAA TATCTCTAGT GCCAGCACTT CAAATATTGC CCGAATAGAA 
GAAATGGAGA GACTGTTGAA GCAGGCTCAT GCAGAGAAGA CCAGGCTGCT TGAATCCAGG 
GAACGAGAAA TGGAAGCCAA GAAACGCGCT CTGGAAGAAG AAAAACGACG TCGAGAACTC 
CTGGAAAAAC GGTTGCAGGA AGAAACTAGC CAGAGGCAGA AGTTAATTGA AAAGGAAGTA 
AAAATAAGGG AGAAACAAAG GGCACAGGCT CGACCTCTGA CCCGCTATCT GCCTGTGCGG 
AAGGAAGACT TTGATTTGCG GAGCCACGTA GAGACTGCTG GCCACAATAT CGATACGTGT 
TATCATGTGT CAATCACAGA GAAGACCTGC AGAGGATTCC TCATCAAAAT GGGTGGAAAA 
ATTAAAACCT GGAAAAAACG TTGGTTTGTT TTTGATCGGA ACAAGCGCAC CTTCTCTTAT 
TATGCAGACA AGCATGAAGC TAAATTAAAA GGAGTAATAT ACTTTCAAGC CATTGAAGAA 
GTATATTATG ATCACCTCAA GAACGCTAAT AAGAGTCCTA ATCCTTTACT TACCTTTAGC 
GTCAAGACAC ATGACAGAAT CTACTACATG GTGGCCCCGT CACCAGAAGC CATGCGGATC 
TGGATGGACG TTATAGTGAC TGGGGCAGAA GGCTACACTC ACTTCTTGTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139686.1
CDS	361..4134
Translation	MEEDNTQRKDTKEEGVGDRQPVDSNKIMAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGKSKKGRMNFSAGAVSNSKSSSVKGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283901.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283901.1

ORF Insert Sequence:

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGCA TTTTAGAAGT 
CTGGAAGAAA GGAAAAAACA GCACCAGGAA GGCCTCTATC TGAGTGACAC TTTGCCTCGA 
AAGAAAACCA CACCTTCCAT ATCCCCACAT TTCAGCAGTG CTACTATGGG GAGAAGCACC 
ACCCCAAAGG GCCACCTGCC CCTGGGACAG AGCAACAGCT GTGGCAGCGT GCTCCCCCAC 
TCGCTAGCAA CCATGACCAA AGACTCAGAA TCTCGGAGGA TGCTCAGAGG GAAGTCCAAG 
AAAGGAAGAA TGAATTTCTC AGCAGGAGCA GTTAGTAATT CCAAAAGTTC AAGTGTCAAA 
GGATATAATC ACCAACAGAT GAGTGAAGGA CAAAGGCAGA AGTCTTCTGA ATTTTGCAAC 
CGCACAGCAT CTGAATCGAA TGTCTACTTG AACAGTTTCC ACTACCCAGA TCACAGCTAC 
AAGGACCAGG CCTTTGATAC TCTGAGCCTA GACAGCTCTG ACAGCATGGA GACCAGCATC 
TCTGCCTGCT CACCTGACAA TATCTCTAGT GCCAGCACTT CAAATATTGC CCGAATAGAA 
GAAATGGAGA GACTGTTGAA GCAGGCTCAT GCAGAGAAGA CCAGGCTGCT TGAATCCAGG 
GAACGAGAAA TGGAAGCCAA GAAACGCGCT CTGGAAGAAG AAAAACGACG TCGAGAACTC 
CTGGAAAAAC GGTTGCAGGA AGAAACTAGC CAGAGGCAGA AGTTAATTGA AAAGGAAGTA 
AAAATAAGGG AGAAACAAAG GGCACAGGCT CGACCTCTGA CCCGCTATCT GCCTGTGCGG 
AAGGAAGACT TTGATTTGCG GAGCCACGTA GAGACTGCTG GCCACAATAT CGATACGTGT 
TATCATGTGT CAATCACAGA GAAGACCTGC AGAGGATTCC TCATCAAAAT GGGTGGAAAA 
ATTAAAACCT GGAAAAAACG TTGGTTTGTT TTTGATCGGA ACAAGCGCAC CTTCTCTTAT 
TATGCAGACA AGCATGAAGC TAAATTAAAA GGAGTAATAT ACTTTCAAGC CATTGAAGAA 
GTATATTATG ATCACCTCAA GAACGCTAAT AAGAGTCCTA ATCCTTTACT TACCTTTAGC 
GTCAAGACAC ATGACAGAAT CTACTACATG GTGGCCCCGT CACCAGAAGC CATGCGGATC 
TGGATGGACG TTATAGTGAC TGGGGCAGAA GGCTACACTC ACTTCTTGTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283905.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139690.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X17, mRNA.

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CloneID	OBu42743
Clone ID Related Accession (Same CDS sequence)	XM_025283905.1
Accession Version	XM_025283905.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGATGCCGA CCTACTGGAT 
GTTGAGAGCA AACACTTTGA AGACCTGGAA TTCCAGCAGC TTGAGCGTGA AAGCCGTCTA 
GATGAGGAAA AGGAGAACCT GACCCAGCAG CTCCTGCGTG AAGTGGCTGA GTATCAACGG 
AACATTGTTA CTAGAAAGGA AAAAATTTCT GCATTGAAAA AGCAAGCTAA TCACATTGTC 
CAGCAGGCTC AACGAGAACA AGATCATTTT GTGAAAGAAA AGAATAATTT AATAATGATG 
CTGCAGAGAG AAAAGGAAAA TCTTTGTAAT TTGGAAAAGA AATACTCCAG CCTCTCTGGG 
GGGAAAGGGT TTCCTGTCAC CCCCAATACT CTGAAAGAGG GCTATATCAT TGTAAATGAG 
ATTAATGAGT CATGTGGCAG TTCCACGAAT CTCCCCCCTT CCACTCAGTT TCCTGCTGAT 
GCTGCTGCTG CTGCCACTGG GCCTACCACA GCTGTGCTGA TGAGCCAGCC ACAAAGTAAA 
GAGCATTTTA GAAGTCTGGA AGAAAGGAAA AAACAGCACC AGGAAGGCCT CTATCTGAGT 
GACACTTTGC CTCGAAAGAA AACCACACCT TCCATATCCC CACATTTCAG CAGTGCTACT 
ATGGGGAGAA GCACCACCCC AAAGGGCCAC CTGCCCCTGG GACAGAGCAA CAGCTGTGGC 
AGCGTGCTCC CCCACTCGCT AGCAACCATG ACCAAAGACT CAGAATCTCG GAGGATGCTC 
AGAGGATATA ATCACCAACA GATGAGTGAA GGACAAAGGC AGAAGTCTTC TGAATTTTGC 
AACCGCACAG CATCTGAATC GAATGTCTAC TTGAACAGTT TCCACTACCC AGATCACAGC 
TACAAGGACC AGGCCTTTGA TACTCTGAGC CTAGACAGCT CTGACAGCAT GGAGACCAGC 
ATCTCTGCCT GCTCACCTGA CAATATCTCT AGTGCCAGCA CTTCAAATAT TGCCCGAATA 
GAAGAAATGG AGAGACTGTT GAAGCAGGCT CATGCAGAGA AGACCAGGCT GCTTGAATCC 
AGGGAACGAG AAATGGAAGC CAAGAAACGC GCTCTGGAAG AAGAAAAACG ACGTCGAGAA 
CTCCTGGAAA AACGGTTGCA GGAAGAAACT AGCCAGAGGC AGAAGTTAAT TGAAAAGGAA 
GTAAAAATAA GGGAGAAACA AAGGGCACAG GCTCGACCTC TGACCCGCTA TCTGCCTGTG 
CGGAAGGAAG ACTTTGATTT GCGGAGCCAC GTAGAGACTG CTGGCCACAA TATCGATACG 
TGTTATCATG TGTCAATCAC AGAGAAGACC TGCAGAGGAT TCCTCATCAA AATGGGTGGA 
AAAATTAAAA CCTGGAAAAA ACGTTGGTTT GTTTTTGATC GGAACAAGCG CACCTTCTCT 
TATTATGCAG ACAAGCATGA AGCTAAATTA AAAGGAGTAA TATACTTTCA AGCCATTGAA 
GAAGTATATT ATGATCACCT CAAGAACGCT AATAAGAGTC CTAATCCTTT ACTTACCTTT 
AGCGTCAAGA CACATGACAG AATCTACTAC ATGGTGGCCC CGTCACCAGA AGCCATGCGG 
ATCTGGATGG ACGTTATAGT GACTGGGGCA GAAGGCTACA CTCACTTCTT GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139690.1
CDS	359..4075
Translation	MEEDNTQRKDTKEEGVGDRQPVDSNKIMAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEGYIIVNEINESCGSSTNLPPSTQFPADAAAAATGPTTAVLMSQPQSKEHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283905.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283905.1

ORF Insert Sequence:

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGATGCCGA CCTACTGGAT 
GTTGAGAGCA AACACTTTGA AGACCTGGAA TTCCAGCAGC TTGAGCGTGA AAGCCGTCTA 
GATGAGGAAA AGGAGAACCT GACCCAGCAG CTCCTGCGTG AAGTGGCTGA GTATCAACGG 
AACATTGTTA CTAGAAAGGA AAAAATTTCT GCATTGAAAA AGCAAGCTAA TCACATTGTC 
CAGCAGGCTC AACGAGAACA AGATCATTTT GTGAAAGAAA AGAATAATTT AATAATGATG 
CTGCAGAGAG AAAAGGAAAA TCTTTGTAAT TTGGAAAAGA AATACTCCAG CCTCTCTGGG 
GGGAAAGGGT TTCCTGTCAC CCCCAATACT CTGAAAGAGG GCTATATCAT TGTAAATGAG 
ATTAATGAGT CATGTGGCAG TTCCACGAAT CTCCCCCCTT CCACTCAGTT TCCTGCTGAT 
GCTGCTGCTG CTGCCACTGG GCCTACCACA GCTGTGCTGA TGAGCCAGCC ACAAAGTAAA 
GAGCATTTTA GAAGTCTGGA AGAAAGGAAA AAACAGCACC AGGAAGGCCT CTATCTGAGT 
GACACTTTGC CTCGAAAGAA AACCACACCT TCCATATCCC CACATTTCAG CAGTGCTACT 
ATGGGGAGAA GCACCACCCC AAAGGGCCAC CTGCCCCTGG GACAGAGCAA CAGCTGTGGC 
AGCGTGCTCC CCCACTCGCT AGCAACCATG ACCAAAGACT CAGAATCTCG GAGGATGCTC 
AGAGGATATA ATCACCAACA GATGAGTGAA GGACAAAGGC AGAAGTCTTC TGAATTTTGC 
AACCGCACAG CATCTGAATC GAATGTCTAC TTGAACAGTT TCCACTACCC AGATCACAGC 
TACAAGGACC AGGCCTTTGA TACTCTGAGC CTAGACAGCT CTGACAGCAT GGAGACCAGC 
ATCTCTGCCT GCTCACCTGA CAATATCTCT AGTGCCAGCA CTTCAAATAT TGCCCGAATA 
GAAGAAATGG AGAGACTGTT GAAGCAGGCT CATGCAGAGA AGACCAGGCT GCTTGAATCC 
AGGGAACGAG AAATGGAAGC CAAGAAACGC GCTCTGGAAG AAGAAAAACG ACGTCGAGAA 
CTCCTGGAAA AACGGTTGCA GGAAGAAACT AGCCAGAGGC AGAAGTTAAT TGAAAAGGAA 
GTAAAAATAA GGGAGAAACA AAGGGCACAG GCTCGACCTC TGACCCGCTA TCTGCCTGTG 
CGGAAGGAAG ACTTTGATTT GCGGAGCCAC GTAGAGACTG CTGGCCACAA TATCGATACG 
TGTTATCATG TGTCAATCAC AGAGAAGACC TGCAGAGGAT TCCTCATCAA AATGGGTGGA 
AAAATTAAAA CCTGGAAAAA ACGTTGGTTT GTTTTTGATC GGAACAAGCG CACCTTCTCT 
TATTATGCAG ACAAGCATGA AGCTAAATTA AAAGGAGTAA TATACTTTCA AGCCATTGAA 
GAAGTATATT ATGATCACCT CAAGAACGCT AATAAGAGTC CTAATCCTTT ACTTACCTTT 
AGCGTCAAGA CACATGACAG AATCTACTAC ATGGTGGCCC CGTCACCAGA AGCCATGCGG 
ATCTGGATGG ACGTTATAGT GACTGGGGCA GAAGGCTACA CTCACTTCTT GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283908.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139693.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OBu42744
Clone ID Related Accession (Same CDS sequence)	XM_025283908.1
Accession Version	XM_025283908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT 
GTAAATGAGA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTT 
CCTGCTGATG CTGCTGCTGC TGCCACTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA 
CAAAGTAAAG AGGGCCACCT GCCCCTGGGA CAGAGCAACA GCTGTGGCAG CGTGCTCCCC 
CACTCGCTAG CAACCATGAC CAAAGACTCA GAATCTCGGA GGATGCTCAG AGGATATAAT 
CACCAACAGA TGAGTGAAGG ACAAAGGCAG AAGTCTTCTG AATTTTGCAA CCGCACAGCA 
TCTGAATCGA ATGTCTACTT GAACAGTTTC CACTACCCAG ATCACAGCTA CAAGGACCAG 
GCCTTTGATA CTCTGAGCCT AGACAGCTCT GACAGCATGG AGACCAGCAT CTCTGCCTGC 
TCACCTGACA ATATCTCTAG TGCCAGCACT TCAAATATTG CCCGAATAGA AGAAATGGAG 
AGACTGTTGA AGCAGGCTCA TGCAGAGAAG ACCAGGCTGC TTGAATCCAG GGAACGAGAA 
ATGGAAGCCA AGAAACGCGC TCTGGAAGAA GAAAAACGAC GTCGAGAACT CCTGGAAAAA 
CGGTTGCAGG AAGAAACTAG CCAGAGGCAG AAGTTAATTG AAAAGGAAGT AAAAATAAGG 
GAGAAACAAA GGGCACAGGC TCGACCTCTG ACCCGCTATC TGCCTGTGCG GAAGGAAGAC 
TTTGATTTGC GGAGCCACGT AGAGACTGCT GGCCACAATA TCGATACGTG TTATCATGTG 
TCAATCACAG AGAAGACCTG CAGAGGATTC CTCATCAAAA TGGGTGGAAA AATTAAAACC 
TGGAAAAAAC GTTGGTTTGT TTTTGATCGG AACAAGCGCA CCTTCTCTTA TTATGCAGAC 
AAGCATGAAG CTAAATTAAA AGGAGTAATA TACTTTCAAG CCATTGAAGA AGTATATTAT 
GATCACCTCA AGAACGCTAA TAAGAGTCCT AATCCTTTAC TTACCTTTAG CGTCAAGACA 
CATGACAGAA TCTACTACAT GGTGGCCCCG TCACCAGAAG CCATGCGGAT CTGGATGGAC 
GTTATAGTGA CTGGGGCAGA AGGCTACACT CACTTCTTGT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139693.1
CDS	357..4061
Translation	MEEDNTQRKDTKEEGVGDRQPVDSNKIMAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEGYIIVNEINESCGSSTNLPPSTQFPADAAAAATGPTTAVLMSQPQSKEGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283908.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283908.1

ORF Insert Sequence:

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT 
GTAAATGAGA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTT 
CCTGCTGATG CTGCTGCTGC TGCCACTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA 
CAAAGTAAAG AGGGCCACCT GCCCCTGGGA CAGAGCAACA GCTGTGGCAG CGTGCTCCCC 
CACTCGCTAG CAACCATGAC CAAAGACTCA GAATCTCGGA GGATGCTCAG AGGATATAAT 
CACCAACAGA TGAGTGAAGG ACAAAGGCAG AAGTCTTCTG AATTTTGCAA CCGCACAGCA 
TCTGAATCGA ATGTCTACTT GAACAGTTTC CACTACCCAG ATCACAGCTA CAAGGACCAG 
GCCTTTGATA CTCTGAGCCT AGACAGCTCT GACAGCATGG AGACCAGCAT CTCTGCCTGC 
TCACCTGACA ATATCTCTAG TGCCAGCACT TCAAATATTG CCCGAATAGA AGAAATGGAG 
AGACTGTTGA AGCAGGCTCA TGCAGAGAAG ACCAGGCTGC TTGAATCCAG GGAACGAGAA 
ATGGAAGCCA AGAAACGCGC TCTGGAAGAA GAAAAACGAC GTCGAGAACT CCTGGAAAAA 
CGGTTGCAGG AAGAAACTAG CCAGAGGCAG AAGTTAATTG AAAAGGAAGT AAAAATAAGG 
GAGAAACAAA GGGCACAGGC TCGACCTCTG ACCCGCTATC TGCCTGTGCG GAAGGAAGAC 
TTTGATTTGC GGAGCCACGT AGAGACTGCT GGCCACAATA TCGATACGTG TTATCATGTG 
TCAATCACAG AGAAGACCTG CAGAGGATTC CTCATCAAAA TGGGTGGAAA AATTAAAACC 
TGGAAAAAAC GTTGGTTTGT TTTTGATCGG AACAAGCGCA CCTTCTCTTA TTATGCAGAC 
AAGCATGAAG CTAAATTAAA AGGAGTAATA TACTTTCAAG CCATTGAAGA AGTATATTAT 
GATCACCTCA AGAACGCTAA TAAGAGTCCT AATCCTTTAC TTACCTTTAG CGTCAAGACA 
CATGACAGAA TCTACTACAT GGTGGCCCCG TCACCAGAAG CCATGCGGAT CTGGATGGAC 
GTTATAGTGA CTGGGGCAGA AGGCTACACT CACTTCTTGT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283911.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139696.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OBu42745
Clone ID Related Accession (Same CDS sequence)	XM_025283911.1
Accession Version	XM_025283911.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CCACCTGCCC 
CTGGGACAGA GCAACAGCTG TGGCAGCGTG CTCCCCCACT CGCTAGCAAC CATGACCAAA 
GACTCAGAAT CTCGGAGGAT GCTCAGAGGG AAGTCCAAGA AAGGAAGAAT GAATTTCTCA 
GCAGGAGCAG TTAGTAATTC CAAAAGTTCA AGTGTCAAAG GATATAATCA CCAACAGATG 
AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA TTTTGCAACC GCACAGCATC TGAATCGAAT 
GTCTACTTGA ACAGTTTCCA CTACCCAGAT CACAGCTACA AGGACCAGGC CTTTGATACT 
CTGAGCCTAG ACAGCTCTGA CAGCATGGAG ACCAGCATCT CTGCCTGCTC ACCTGACAAT 
ATCTCTAGTG CCAGCACTTC AAATATTGCC CGAATAGAAG AAATGGAGAG ACTGTTGAAG 
CAGGCTCATG CAGAGAAGAC CAGGCTGCTT GAATCCAGGG AACGAGAAAT GGAAGCCAAG 
AAACGCGCTC TGGAAGAAGA AAAACGACGT CGAGAACTCC TGGAAAAACG GTTGCAGGAA 
GAAACTAGCC AGAGGCAGAA GTTAATTGAA AAGGAAGTAA AAATAAGGGA GAAACAAAGG 
GCACAGGCTC GACCTCTGAC CCGCTATCTG CCTGTGCGGA AGGAAGACTT TGATTTGCGG 
AGCCACGTAG AGACTGCTGG CCACAATATC GATACGTGTT ATCATGTGTC AATCACAGAG 
AAGACCTGCA GAGGATTCCT CATCAAAATG GGTGGAAAAA TTAAAACCTG GAAAAAACGT 
TGGTTTGTTT TTGATCGGAA CAAGCGCACC TTCTCTTATT ATGCAGACAA GCATGAAGCT 
AAATTAAAAG GAGTAATATA CTTTCAAGCC ATTGAAGAAG TATATTATGA TCACCTCAAG 
AACGCTAATA AGAGTCCTAA TCCTTTACTT ACCTTTAGCG TCAAGACACA TGACAGAATC 
TACTACATGG TGGCCCCGTC ACCAGAAGCC ATGCGGATCT GGATGGACGT TATAGTGACT 
GGGGCAGAAG GCTACACTCA CTTCTTGTTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139696.1
CDS	360..3992
Translation	MEEDNTQRKDTKEEGVGDRQPVDSNKIMAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGKSKKGRMNFSAGAVSNSKSSSVKGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283911.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283911.1

ORF Insert Sequence:

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CCACCTGCCC 
CTGGGACAGA GCAACAGCTG TGGCAGCGTG CTCCCCCACT CGCTAGCAAC CATGACCAAA 
GACTCAGAAT CTCGGAGGAT GCTCAGAGGG AAGTCCAAGA AAGGAAGAAT GAATTTCTCA 
GCAGGAGCAG TTAGTAATTC CAAAAGTTCA AGTGTCAAAG GATATAATCA CCAACAGATG 
AGTGAAGGAC AAAGGCAGAA GTCTTCTGAA TTTTGCAACC GCACAGCATC TGAATCGAAT 
GTCTACTTGA ACAGTTTCCA CTACCCAGAT CACAGCTACA AGGACCAGGC CTTTGATACT 
CTGAGCCTAG ACAGCTCTGA CAGCATGGAG ACCAGCATCT CTGCCTGCTC ACCTGACAAT 
ATCTCTAGTG CCAGCACTTC AAATATTGCC CGAATAGAAG AAATGGAGAG ACTGTTGAAG 
CAGGCTCATG CAGAGAAGAC CAGGCTGCTT GAATCCAGGG AACGAGAAAT GGAAGCCAAG 
AAACGCGCTC TGGAAGAAGA AAAACGACGT CGAGAACTCC TGGAAAAACG GTTGCAGGAA 
GAAACTAGCC AGAGGCAGAA GTTAATTGAA AAGGAAGTAA AAATAAGGGA GAAACAAAGG 
GCACAGGCTC GACCTCTGAC CCGCTATCTG CCTGTGCGGA AGGAAGACTT TGATTTGCGG 
AGCCACGTAG AGACTGCTGG CCACAATATC GATACGTGTT ATCATGTGTC AATCACAGAG 
AAGACCTGCA GAGGATTCCT CATCAAAATG GGTGGAAAAA TTAAAACCTG GAAAAAACGT 
TGGTTTGTTT TTGATCGGAA CAAGCGCACC TTCTCTTATT ATGCAGACAA GCATGAAGCT 
AAATTAAAAG GAGTAATATA CTTTCAAGCC ATTGAAGAAG TATATTATGA TCACCTCAAG 
AACGCTAATA AGAGTCCTAA TCCTTTACTT ACCTTTAGCG TCAAGACACA TGACAGAATC 
TACTACATGG TGGCCCCGTC ACCAGAAGCC ATGCGGATCT GGATGGACGT TATAGTGACT 
GGGGCAGAAG GCTACACTCA CTTCTTGTTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283914.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139699.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X20, mRNA.

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CloneID	OBu42746
Clone ID Related Accession (Same CDS sequence)	XM_025283914.1
Accession Version	XM_025283914.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CCACCTGCCC 
CTGGGACAGA GCAACAGCTG TGGCAGCGTG CTCCCCCACT CGCTAGCAAC CATGACCAAA 
GACTCAGAAT CTCGGAGGAT GCTCAGAGGA TATAATCACC AACAGATGAG TGAAGGACAA 
AGGCAGAAGT CTTCTGAATT TTGCAACCGC ACAGCATCTG AATCGAATGT CTACTTGAAC 
AGTTTCCACT ACCCAGATCA CAGCTACAAG GACCAGGCCT TTGATACTCT GAGCCTAGAC 
AGCTCTGACA GCATGGAGAC CAGCATCTCT GCCTGCTCAC CTGACAATAT CTCTAGTGCC 
AGCACTTCAA ATATTGCCCG AATAGAAGAA ATGGAGAGAC TGTTGAAGCA GGCTCATGCA 
GAGAAGACCA GGCTGCTTGA ATCCAGGGAA CGAGAAATGG AAGCCAAGAA ACGCGCTCTG 
GAAGAAGAAA AACGACGTCG AGAACTCCTG GAAAAACGGT TGCAGGAAGA AACTAGCCAG 
AGGCAGAAGT TAATTGAAAA GGAAGTAAAA ATAAGGGAGA AACAAAGGGC ACAGGCTCGA 
CCTCTGACCC GCTATCTGCC TGTGCGGAAG GAAGACTTTG ATTTGCGGAG CCACGTAGAG 
ACTGCTGGCC ACAATATCGA TACGTGTTAT CATGTGTCAA TCACAGAGAA GACCTGCAGA 
GGATTCCTCA TCAAAATGGG TGGAAAAATT AAAACCTGGA AAAAACGTTG GTTTGTTTTT 
GATCGGAACA AGCGCACCTT CTCTTATTAT GCAGACAAGC ATGAAGCTAA ATTAAAAGGA 
GTAATATACT TTCAAGCCAT TGAAGAAGTA TATTATGATC ACCTCAAGAA CGCTAATAAG 
AGTCCTAATC CTTTACTTAC CTTTAGCGTC AAGACACATG ACAGAATCTA CTACATGGTG 
GCCCCGTCAC CAGAAGCCAT GCGGATCTGG ATGGACGTTA TAGTGACTGG GGCAGAAGGC 
TACACTCACT TCTTGTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139699.1
CDS	355..3915
Translation	MEEDNTQRKDTKEEGVGDRQPVDSNKIMAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGYNHQQMSEGQRQKSSEFCNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTSNIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRRELLEKRLQEETSQRQKLIEKEVKIREKQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTCRGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHEAKLKGVIYFQAIEEVYYDHLKNANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL

Target ORF information:

RefSeq Version	XM_025283914.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283914.1

ORF Insert Sequence:

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CCACCTGCCC 
CTGGGACAGA GCAACAGCTG TGGCAGCGTG CTCCCCCACT CGCTAGCAAC CATGACCAAA 
GACTCAGAAT CTCGGAGGAT GCTCAGAGGA TATAATCACC AACAGATGAG TGAAGGACAA 
AGGCAGAAGT CTTCTGAATT TTGCAACCGC ACAGCATCTG AATCGAATGT CTACTTGAAC 
AGTTTCCACT ACCCAGATCA CAGCTACAAG GACCAGGCCT TTGATACTCT GAGCCTAGAC 
AGCTCTGACA GCATGGAGAC CAGCATCTCT GCCTGCTCAC CTGACAATAT CTCTAGTGCC 
AGCACTTCAA ATATTGCCCG AATAGAAGAA ATGGAGAGAC TGTTGAAGCA GGCTCATGCA 
GAGAAGACCA GGCTGCTTGA ATCCAGGGAA CGAGAAATGG AAGCCAAGAA ACGCGCTCTG 
GAAGAAGAAA AACGACGTCG AGAACTCCTG GAAAAACGGT TGCAGGAAGA AACTAGCCAG 
AGGCAGAAGT TAATTGAAAA GGAAGTAAAA ATAAGGGAGA AACAAAGGGC ACAGGCTCGA 
CCTCTGACCC GCTATCTGCC TGTGCGGAAG GAAGACTTTG ATTTGCGGAG CCACGTAGAG 
ACTGCTGGCC ACAATATCGA TACGTGTTAT CATGTGTCAA TCACAGAGAA GACCTGCAGA 
GGATTCCTCA TCAAAATGGG TGGAAAAATT AAAACCTGGA AAAAACGTTG GTTTGTTTTT 
GATCGGAACA AGCGCACCTT CTCTTATTAT GCAGACAAGC ATGAAGCTAA ATTAAAAGGA 
GTAATATACT TTCAAGCCAT TGAAGAAGTA TATTATGATC ACCTCAAGAA CGCTAATAAG 
AGTCCTAATC CTTTACTTAC CTTTAGCGTC AAGACACATG ACAGAATCTA CTACATGGTG 
GCCCCGTCAC CAGAAGCCAT GCGGATCTGG ATGGACGTTA TAGTGACTGG GGCAGAAGGC 
TACACTCACT TCTTGTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB2 ( XM_025283918.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139703.1 Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OBu42747
Clone ID Related Accession (Same CDS sequence)	XM_025283918.1
Accession Version	XM_025283918.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	pleckstrin homology-like domain family B member 2 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT 
GTAAATGAGA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTT 
CCTGCTGATG CTGCTGCTGC TGCCACTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA 
CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT 
TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCTGTGGC TACCTCTGTC 
GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA 
CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA 
GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT 
TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG 
AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA 
TCTCGGAGGA TGCTCAGAGG TCTTTTGTGC ATTCCAGAAG CCTGCAGCCT CAGTCTTTCC 
CTGCTGCAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139703.1
CDS	388..3582
Translation	MEEDNTQRKDTKEEGVGDRQPVDSNKIMAEQSHMQKQLDLQNGSLEAGFVENSLENDSQNMMESLSPKKYSSSLRFKANGDCSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGTKQFSCDGSDKSISMKPPTPLRSTSPSLSGYALGRADFDHFLGRDAERSLRLSEKPPYSKYSSRNKSHDNVYFLGGLEGRKASGSLLTMWNGSSLSDTGSSPINRSGAASMPSSPKQARKISIQDNLTLQPKLSRHRDPASENISLRTRKYSSGSLSHMGAYSRSLPRLHRATENQLAPLSLPPRSSLGNSKRTKLGEKDLPHSIIDNDNYLNFSSLSSGALPYKASASEGSPYVSSTLSVPASPRVARKMLLASTSSCTSDDLDRASYSGTSPGHSFPPGELDRAFAARRNFSCGSVEFDDADLDSLRQASGTPQPVLRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRLETILSLCAEYTKPDSRVSPGTTVADVQKINKELEKLQLSDEESVFEEALVSPDTRYRCHQKSALHDADLAGFGSLSQSSASFLPPRGSRNDELLGDLARTPPPPSSAFLKASGESAYLSILPKTPEGISEEQRIQELTAMEETRIAILNNLEELEQKIKDINDQMEESSRELDMECALLDGEQKSETTELMKEKEILDHLNRKITELEKNIVGEKTKEKVKLDAEREKLERLQELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLERESRLDEEKENLTQQLLREVAEYQRNIVTRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQREKENLCNLEKKYSSLSGGKGFPVTPNTLKEGYIIVNEINESCGSSTNLPPSTQFPADAAAAATGPTTAVLMSQPQSKEQRSSVCPGFMFPSTLSSHPITQPPSAPWPEAVATSVDSFPLNDTPPPLPAKKHRRQQQQDQQHFRSLEERKKQHQEGLYLSDTLPRKKTTPSISPHFSSATMGRSTTPKGHLPLGQSNSCGSVLPHSLATMTKDSESRRMLRGLLCIPEACSLSLSLLQL

Target ORF information:

RefSeq Version	XM_025283918.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis pleckstrin homology like domain family B member 2 (PHLDB2), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283918.1

ORF Insert Sequence:

ATGGAGGAGG ATAATACCCA GAGAAAGGAC ACCAAGGAAG AGGGAGTTGG TGACAGACAG 
CCTGTGGATT CCAACAAGAT TATGGCAGAG CAGAGCCACA TGCAGAAGCA GCTGGATTTA 
CAGAATGGTA GCTTAGAGGC AGGCTTTGTG GAGAACTCCC TCGAAAACGA TTCCCAAAAC 
ATGATGGAGA GCCTGAGCCC AAAGAAATAT TCTTCCAGTC TGAGATTTAA AGCCAATGGA 
GACTGTTCTG GCTCCTATTT AACCCTCTCA CAACCTGTGC CTGCTAAAAG AAGCCCTTCT 
CCTTTGGGAA CCAGTGTCAG AAGTAGCCCT TCTTTGGCCA AAATTCAGGG AACAAAGCAG 
TTCTCTTGCG ATGGAAGTGA CAAAAGTATT TCCATGAAAC CGCCCACCCC TTTACGCAGC 
ACCTCACCCT CCCTTAGTGG ATATGCACTT GGGAGAGCAG ACTTTGATCA TTTTCTTGGC 
CGGGACGCCG AAAGGTCCTT GAGGCTTTCA GAGAAGCCTC CCTATTCCAA ATATAGCTCA 
AGGAATAAAT CCCATGACAA TGTCTACTTT CTCGGAGGGC TGGAAGGCCG AAAAGCATCT 
GGCTCGCTCC TGACCATGTG GAATGGAAGT TCCCTGAGTG ACACTGGCTC CTCGCCCATC 
AACAGGTCAG GGGCGGCCAG CATGCCTTCA AGCCCAAAGC AAGCCAGGAA AATAAGCATC 
CAGGACAACC TGACACTTCA GCCCAAGTTG AGCAGACACC GGGACCCGGC ATCTGAAAAC 
ATCAGTTTAA GAACCAGGAA GTACTCTAGC GGCAGCCTGA GTCACATGGG CGCCTACAGC 
CGCTCTCTCC CCAGGTTGCA TAGAGCCACC GAGAACCAGT TGGCACCCCT CAGTCTGCCT 
CCAAGAAGCT CTCTGGGCAA TTCCAAACGA ACGAAACTGG GGGAAAAGGA TCTACCTCAT 
AGCATTATAG ACAATGACAA TTACCTGAAT TTTTCTTCTT TGAGCTCAGG AGCTTTGCCC 
TATAAAGCCT CTGCCTCCGA GGGCAGTCCG TACGTGAGTT CCACCCTCAG TGTGCCTGCC 
AGTCCTCGAG TGGCTCGCAA GATGCTTCTG GCCTCCACCT CCTCCTGCAC CTCTGACGAC 
CTTGACAGGG CTTCCTACTC GGGTACCAGC CCTGGTCATT CGTTTCCTCC TGGGGAGCTG 
GACCGAGCGT TTGCAGCCAG AAGGAACTTC TCGTGTGGAT CTGTCGAGTT TGATGATGCA 
GACTTGGACA GCCTCAGACA GGCCTCAGGA ACCCCCCAGC CTGTCCTGCG GGAACGGAAA 
AGCAGCATTA GCTCCATTTC CGGACGAGAC GACCTGATGG ATTATCACCG GCGGCAGAGG 
GAGGAGAGAC TCAGGGAGCA GGAGATGGAG CGATTGGAGA GACAGCGTCT GGAGACCATC 
CTCAGCCTCT GTGCTGAGTA CACAAAGCCT GACAGTCGCG TGTCCCCCGG CACCACCGTG 
GCAGATGTGC AGAAAATCAA TAAAGAGCTG GAGAAACTGC AGCTCTCCGA TGAGGAGTCT 
GTGTTTGAAG AAGCCCTGGT GAGCCCGGAC ACCAGGTATA GGTGCCACCA GAAAAGCGCT 
CTCCACGACG CGGACCTGGC AGGCTTTGGG AGCCTCAGTC AGAGCAGTGC CAGCTTCCTC 
CCGCCACGGG GCTCCAGGAA TGATGAACTG CTCGGGGACC TCGCCAGGAC TCCCCCGCCG 
CCCTCCTCTG CCTTTCTGAA AGCTTCCGGC GAGTCTGCTT ACCTAAGTAT CCTACCAAAG 
ACCCCAGAGG GTATAAGTGA AGAACAAAGG ATTCAGGAGT TGACTGCGAT GGAAGAGACC 
CGGATAGCAA TTCTGAACAA CCTTGAAGAA CTTGAACAAA AAATCAAAGA TATAAATGAC 
CAGATGGAGG AATCTTCCAG AGAGCTGGAT ATGGAATGTG CTCTCTTGGA TGGAGAACAA 
AAATCAGAAA CAACTGAACT TATGAAAGAG AAGGAGATTT TGGATCATCT AAACCGGAAA 
ATCACAGAAC TGGAAAAGAA CATTGTTGGT GAAAAGACCA AGGAGAAGGT AAAGCTTGAT 
GCTGAAAGGG AAAAACTAGA GAGGCTTCAG GAGCTTTACT CCGAGCAGAA GACCCAGCTG 
GACAATTGTC CTGAGTCCAT GAGGGAACAG TTACAACAAC AACTGAAGAG GGATGCCGAC 
CTACTGGATG TTGAGAGCAA ACACTTTGAA GACCTGGAAT TCCAGCAGCT TGAGCGTGAA 
AGCCGTCTAG ATGAGGAAAA GGAGAACCTG ACCCAGCAGC TCCTGCGTGA AGTGGCTGAG 
TATCAACGGA ACATTGTTAC TAGAAAGGAA AAAATTTCTG CATTGAAAAA GCAAGCTAAT 
CACATTGTCC AGCAGGCTCA ACGAGAACAA GATCATTTTG TGAAAGAAAA GAATAATTTA 
ATAATGATGC TGCAGAGAGA AAAGGAAAAT CTTTGTAATT TGGAAAAGAA ATACTCCAGC 
CTCTCTGGGG GGAAAGGGTT TCCTGTCACC CCCAATACTC TGAAAGAGGG CTATATCATT 
GTAAATGAGA TTAATGAGTC ATGTGGCAGT TCCACGAATC TCCCCCCTTC CACTCAGTTT 
CCTGCTGATG CTGCTGCTGC TGCCACTGGG CCTACCACAG CTGTGCTGAT GAGCCAGCCA 
CAAAGTAAAG AGCAAAGATC GTCTGTCTGT CCTGGATTTA TGTTTCCTTC CACTCTTTCT 
TCTCATCCTA TCACTCAACC TCCATCAGCC CCTTGGCCTG AAGCTGTGGC TACCTCTGTC 
GATTCTTTTC CTTTAAATGA CACACCTCCT CCTCTACCAG CTAAGAAACA CAGGAGGCAA 
CAGCAGCAGG ACCAGCAGCA TTTTAGAAGT CTGGAAGAAA GGAAAAAACA GCACCAGGAA 
GGCCTCTATC TGAGTGACAC TTTGCCTCGA AAGAAAACCA CACCTTCCAT ATCCCCACAT 
TTCAGCAGTG CTACTATGGG GAGAAGCACC ACCCCAAAGG GCCACCTGCC CCTGGGACAG 
AGCAACAGCT GTGGCAGCGT GCTCCCCCAC TCGCTAGCAA CCATGACCAA AGACTCAGAA 
TCTCGGAGGA TGCTCAGAGG TCTTTTGTGC ATTCCAGAAG CCTGCAGCCT CAGTCTTTCC 
CTGCTGCAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PHLDB2
Entrez Gene ID	102399719
Full Name	pleckstrin homology like domain family B member 2
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PHLDB2 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

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