LOC102399306 gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006072342.1	XP_006072404.1	collagen alpha-5(IV) chain-like isoform X2
XM_006072344.1	XP_006072406.1	collagen alpha-5(IV) chain-like isoform X4
XM_006072342.2	XP_006072404.1	collagen alpha-5(IV) chain isoform X2
XM_006072343.2	XP_006072405.1	collagen alpha-5(IV) chain isoform X3
XM_006072341.1	XP_006072403.1	collagen alpha-5(IV) chain-like isoform X1
XM_006072346.1	XP_006072408.1	collagen alpha-5(IV) chain-like isoform X6
XM_006072341.2	XP_006072403.1	collagen alpha-5(IV) chain isoform X1
XM_006072345.2	XP_006072407.1	collagen alpha-5(IV) chain isoform X5
XM_025276168.1	XP_025131953.1	collagen alpha-5(IV) chain isoform X4
XM_006072346.2	XP_006072408.1	collagen alpha-5(IV) chain isoform X6
XM_006072343.1	XP_006072405.1	collagen alpha-5(IV) chain-like isoform X3
XM_006072345.1	XP_006072407.1	collagen alpha-5(IV) chain-like isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu73558	XM_025276168.1 Latest version!	Bubalus bubalis collagen type IV alpha 5 chain (COL4A5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu26601	XM_006072341.2 Latest version!	Bubalus bubalis collagen type IV alpha 5 chain (COL4A5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBu26602	XM_006072342.2 Latest version!	Bubalus bubalis collagen type IV alpha 5 chain (COL4A5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBu26603	XM_006072343.2 Latest version!	Bubalus bubalis collagen type IV alpha 5 chain (COL4A5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBu26604	XM_006072344.1 Latest version!	Bubalus bubalis collagen alpha-5(IV) chain-like (LOC102399306), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBu26605	XM_006072345.2 Latest version!	Bubalus bubalis collagen type IV alpha 5 chain (COL4A5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBu26606	XM_006072346.2 Latest version!	Bubalus bubalis collagen type IV alpha 5 chain (COL4A5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
OBu26602	XM_006072342.1	Bubalus bubalis collagen alpha-5(IV) chain-like (LOC102399306), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBu26601	XM_006072341.1	Bubalus bubalis collagen alpha-5(IV) chain-like (LOC102399306), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBu26606	XM_006072346.1	Bubalus bubalis collagen alpha-5(IV) chain-like (LOC102399306), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBu26603	XM_006072343.1	Bubalus bubalis collagen alpha-5(IV) chain-like (LOC102399306), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBu26605	XM_006072345.1	Bubalus bubalis collagen alpha-5(IV) chain-like (LOC102399306), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

LOC102399306 ( XM_025276168.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025131953.1 Bubalus bubalis collagen type IV alpha 5 chain (COL4A5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu73558
Clone ID Related Accession (Same CDS sequence)	XM_025276168.1
Accession Version	XM_025276168.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-5(IV) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG TCTACCAGGC 
TTGCATGGAC TACCAGGAGA GAAGGGGGAT CCAGGGCCTC CTGGATTTGA TGTTCCAGGA 
CCTCCTGGTG AGAGAGGCAG CCCAGGGATC CCTGGAGCAC CAGGTCCTAT GGGACCCCCA 
GGATCACCAG GGCTCCCAGG AAAAGCAGGT GCCTCTGGCT TTCCAGGTGC CAAAGGTGAA 
ATGGGTATGA TGGGACCTCC AGGCCCACCA GGACCTTTGG GAATTCCTGG CAGGAGTGGT 
GTTCCTGGTC TCAAAGGTGA TGATGGTTTG CAGGGTCAGC CAGGACTTCC TGGCCCTGCA 
GGAGAGAAAG GTAGTAAAGG AGAGCCTGGC CTTCCAGGCC TTCCTGGGCC TATGGATCCA 
GATCTGCTGG GCTCAAAAGG AGAGAAAGGG GACCCTGGCC TACCAGGTAT TCCTGGAGTT 
GCAGGGCCAA AAGGTTACCA GGGTTTACCT GGAGACCCAG GGCAACCTGG ACTGAGTGGA 
CAACCTGGAT TGCCAGGACC AGCAGGTCCC AAGGGTAACC CTGGTCTCCC TGGAATGCCA 
GGACTTACAG GACCTCCTGG ACTGAAAGGA AACATGGGTG ATATGGGTTT TCCAGGCCCA 
CAGGGTCCAA AAGGGTCTTT CGGACCTCCT GGAGTTCCTG GACAACCTGG CTCCCCAGGA 
TTACCTGGAC AGAAAGGAGA AAAAGGTGAT CCTGGTATTT CAGGCATTGG TCTTCCAGGT 
CTTCCTGGCC CAAAGGGTGA ACCTGGTCTG CCTGGATACC CAGGGAACCC TGGTACCAAA 
GGTTCTATGG GAGAAACTGG TTTGCCTGGA TTACCGGGGA CCCCTGGAGC AAAAGGACAA 
CCAGGCCTTC CTGGATTCCC TGGAACCCCA GGCCTTCCTG GACCAAAAGG TATTAGTGGT 
CCTCCTGGGA ACCCTGGCCT TCCAGGAGAA CCTGGTCCTG TAGGTGGTGG AGGTCGTCCT 
GGGCCACCAG GGCCTCCAGG TGAAAAAGGC AAACCAGGTC AAGATGGTAT TCCTGGACCA 
GCTGGACAGA AGGGTGAACC AGGTCAACCA GGCTTTGGAA TCCCAGGACC CCCTGGACTT 
CCAGGACTTT CTGGTCAAAA GGGTGATGAA GGATTACCTG GCATTCCAGG AAACCCTGGC 
CTTCCAGGTC CAAAGGGTGA ACCAGGCTTT CATGGTTTCC CTGGTCTGCA GGGTCCCCCA 
GGCCCTCCTG GTTCTCCAGG TCCAGCTCTG GAAGGCCCTA AAGGCAGCCC TGGACCCCAA 
GGTCCTCCTG GGAGACCAGG TCCTACAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA 
CCTGGAAGTG GGGGTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT 
TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC 
CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGTCAGA GTATTGTAAT CAAAGGAGAT 
GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA 
CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT 
CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA 
ACCTCTTCTA TCGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA 
CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT 
TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT 
GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG 
ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG 
GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG 
CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG 
GCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025131953.1
CDS	328..5403
Translation	MKLRGVSLAAGLSLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGPAGPPGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGVKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGLPGPPGLVIPRPGTGVTVGEKGNIGLPGLPGDKGDRGFPGIQGPPGLPGPPGPAVIGPPGPPGFPGERGQKGDEGPPGISIPGSPGLDGQPGAPGPPGPPGPPGPHIPPSDKICEAGPPGPPGSPGDRGLQGEQGMKGDKGDTCFNCIGTGVSGPPGQPGLPGLPGPPGSLGFPGQKGEKGHAGATGPKGLTGIPGAPGAPGFPGPKGEPGDILTFPGMKGDKGDLGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGMAFKGERGPPGNPGLPGLPGNRGPMGPVGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGHPGRDGEVGLPGEPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGSPGRIGLEGPSGPPGFPGPKGEPGFGLPGPPGPPGLPGFKGILGPKGDRGFPGPQGPPGQAGLDGLPGPKGDIGPNGQPGPIGPPGLPGTGFQGPPGPPGIPGPIGQPGLPGLHGLPGEKGDPGPPGFDVPGPPGERGSPGIPGAPGPMGPPGSPGLPGKAGASGFPGAKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGLPGPMDPDLLGSKGEKGDPGLPGIPGVAGPKGYQGLPGDPGQPGLSGQPGLPGPAGPKGNPGLPGMPGLTGPPGLKGNMGDMGFPGPQGPKGSFGPPGVPGQPGSPGLPGQKGEKGDPGISGIGLPGLPGPKGEPGLPGYPGNPGTKGSMGETGLPGLPGTPGAKGQPGLPGFPGTPGLPGPKGISGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDEGLPGIPGNPGLPGPKGEPGFHGFPGLQGPPGPPGSPGPALEGPKGSPGPQGPPGRPGPTGPPGPEGPRGLPGSGGIKGERGNPGQPGQPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSTGPEGDPGLIGPPGPPGLPGPSGQSIVIKGDAGPPGIPGQPGLKGLQGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTIQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVADMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_025276168.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen type IV alpha 5 chain (COL4A5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025276168.1

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG TCTACCAGGC 
TTGCATGGAC TACCAGGAGA GAAGGGGGAT CCAGGGCCTC CTGGATTTGA TGTTCCAGGA 
CCTCCTGGTG AGAGAGGCAG CCCAGGGATC CCTGGAGCAC CAGGTCCTAT GGGACCCCCA 
GGATCACCAG GGCTCCCAGG AAAAGCAGGT GCCTCTGGCT TTCCAGGTGC CAAAGGTGAA 
ATGGGTATGA TGGGACCTCC AGGCCCACCA GGACCTTTGG GAATTCCTGG CAGGAGTGGT 
GTTCCTGGTC TCAAAGGTGA TGATGGTTTG CAGGGTCAGC CAGGACTTCC TGGCCCTGCA 
GGAGAGAAAG GTAGTAAAGG AGAGCCTGGC CTTCCAGGCC TTCCTGGGCC TATGGATCCA 
GATCTGCTGG GCTCAAAAGG AGAGAAAGGG GACCCTGGCC TACCAGGTAT TCCTGGAGTT 
GCAGGGCCAA AAGGTTACCA GGGTTTACCT GGAGACCCAG GGCAACCTGG ACTGAGTGGA 
CAACCTGGAT TGCCAGGACC AGCAGGTCCC AAGGGTAACC CTGGTCTCCC TGGAATGCCA 
GGACTTACAG GACCTCCTGG ACTGAAAGGA AACATGGGTG ATATGGGTTT TCCAGGCCCA 
CAGGGTCCAA AAGGGTCTTT CGGACCTCCT GGAGTTCCTG GACAACCTGG CTCCCCAGGA 
TTACCTGGAC AGAAAGGAGA AAAAGGTGAT CCTGGTATTT CAGGCATTGG TCTTCCAGGT 
CTTCCTGGCC CAAAGGGTGA ACCTGGTCTG CCTGGATACC CAGGGAACCC TGGTACCAAA 
GGTTCTATGG GAGAAACTGG TTTGCCTGGA TTACCGGGGA CCCCTGGAGC AAAAGGACAA 
CCAGGCCTTC CTGGATTCCC TGGAACCCCA GGCCTTCCTG GACCAAAAGG TATTAGTGGT 
CCTCCTGGGA ACCCTGGCCT TCCAGGAGAA CCTGGTCCTG TAGGTGGTGG AGGTCGTCCT 
GGGCCACCAG GGCCTCCAGG TGAAAAAGGC AAACCAGGTC AAGATGGTAT TCCTGGACCA 
GCTGGACAGA AGGGTGAACC AGGTCAACCA GGCTTTGGAA TCCCAGGACC CCCTGGACTT 
CCAGGACTTT CTGGTCAAAA GGGTGATGAA GGATTACCTG GCATTCCAGG AAACCCTGGC 
CTTCCAGGTC CAAAGGGTGA ACCAGGCTTT CATGGTTTCC CTGGTCTGCA GGGTCCCCCA 
GGCCCTCCTG GTTCTCCAGG TCCAGCTCTG GAAGGCCCTA AAGGCAGCCC TGGACCCCAA 
GGTCCTCCTG GGAGACCAGG TCCTACAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA 
CCTGGAAGTG GGGGTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT 
TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC 
CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGTCAGA GTATTGTAAT CAAAGGAGAT 
GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA 
CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT 
CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA 
ACCTCTTCTA TCGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA 
CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT 
TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT 
GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG 
ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG 
GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG 
CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG 
GCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102399306 ( XM_006072341.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006072403.1 Bubalus bubalis collagen alpha-5(IV) chain-like (LOC102399306), transcript variant X1, mRNA.

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CloneID	OBu26601
Clone ID Related Accession (Same CDS sequence)	XM_006072341.1 , XM_006072341.2
Accession Version	XM_006072341.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-5(IV) chain-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785484.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG TCTACCAGGC 
TTGCATGGAC TACCAGGAGA GAAGGGGGAT CCAGGGCCTC CTGGATTTGA TGTTCCAGGA 
CCTCCTGGTG AGAGAGGCAG CCCAGGGATC CCTGGAGCAC CAGGTCCTAT GGGACCCCCA 
GGATCACCAG GGCTCCCAGG AAAAGCAGGT GCCTCTGGCT TTCCAGGTGC CAAAGGTGAA 
ATGGGTATGA TGGGACCTCC AGGCCCACCA GGACCTTTGG GAATTCCTGG CAGGAGTGGT 
GTTCCTGGTC TCAAAGGTGA TGATGGTTTG CAGGGTCAGC CAGGACTTCC TGGCCCTGCA 
GGAGAGAAAG GTAGTAAAGG AGAGCCTGGC CTTCCAGGCC TTCCTGGGCC TATGGATCCA 
GATCTGCTGG GCTCAAAAGG AGAGAAAGGG GACCCTGGCC TACCAGGTAT TCCTGGAGTT 
GCAGGGCCAA AAGGTTACCA GGGTTTACCT GGAGACCCAG GGCAACCTGG ACTGAGTGGA 
CAACCTGGAT TGCCAGGACC AGCAGGTCCC AAGGGTAACC CTGGTCTCCC TGGAATGCCA 
GGACTTACAG GACCTCCTGG ACTGAAAGGA AACATGGGTG ATATGGGTTT TCCAGGCCCA 
CAGGGTCCAA AAGGGTCTTT CGGACCTCCT GGAGTTCCTG GACAACCTGG CTCCCCAGGA 
TTACCTGGAC AGAAAGGAGA AAAAGGTGAT CCTGGTATTT CAGGCATTGG TCTTCCAGGT 
CTTCCTGGCC CAAAGGGTGA ACCTGGTCTG CCTGGATACC CAGGGAACCC TGGTACCAAA 
GGTTCTATGG GAGAAACTGG TTTGCCTGGA TTACCGGGGA CCCCTGGAGC AAAAGGACAA 
CCAGGCCTTC CTGGATTCCC TGGAACCCCA GGCCTTCCTG GACCAAAAGG TATTAGTGGT 
CCTCCTGGGA ACCCTGGCCT TCCAGGAGAA CCTGGTCCTG TAGGTGGTGG AGGTCGTCCT 
GGGCCACCAG GGCCTCCAGG TGAAAAAGGC AAACCAGGTC AAGATGGTAT TCCTGGACCA 
GCTGGACAGA AGGGTGAACC AGGTCAACCA GGCTTTGGAA TCCCAGGACC CCCTGGACTT 
CCAGGACTTT CTGGTCAAAA GGGTGATGAA GGATTACCTG GCATTCCAGG AAACCCTGGC 
CTTCCAGGTC CAAAGGGTGA ACCAGGCTTT CATGGTTTCC CTGGTCTGCA GGGTCCCCCA 
GGCCCTCCTG GTTCTCCAGG TCCAGCTCTG GAAGGCCCTA AAGGCAGCCC TGGACCCCAA 
GGTCCTCCTG GGAGACCAGG TCCTACAGGT TTTCAAGGTC CACCAGGTCC AGAAGGTCCC 
CGGGGTCTAC CTGGAAGTGG GGGTATTAAA GGAGAAAGAG GTAACCCAGG CCAACCTGGG 
CAACCTGGTT TGCCTGGTTT GAAAGGTGAT CAAGGTCCTC CAGGACTCCA GGGTAACCCT 
GGCCGGCCAG GTCTCAATGG AATGAAAGGA GATCCTGGTC TCCCTGGTGT TCCAGGATTT 
CCAGGCATGA AAGGACCCAG TGGAGTACCT GGTTCAACTG GCCCTGAGGG GGATCCAGGA 
CTTATTGGCC CACCAGGTCC CCCTGGATTA CCTGGTCCTT CAGGTCAGAG TATTGTAATC 
AAAGGAGATG CTGGCCCACC AGGGATTCCA GGCCAGCCTG GATTAAAAGG TTTACAAGGA 
CTCCCAGGAC CTCAGGGTTT ACCAGGTCCA ATTGGCCCTC CAGGAGATCC TGGACGCAAT 
GGACTCCCTG GCTTTGATGG TGCAGGAGGA CGCAAAGGAG ACCCAGGTTT GCCAGGCCAG 
CCAGGTACTC GTGGCTTGGA TGGTCCCCCC GGGCCAGATG GATTGCAGGG TCCTCCAGGT 
CCCCCTGGAA CCTCTTCTAT CGCCCATGGG TTTCTCATCA CACGTCACAG CCAGACAACA 
GATGCTCCAC AATGCCCACA GGGAACCATC CAGGTGTATG AAGGCTTTTC TCTCCTGTAT 
GTACAAGGAA ATAAAAGAGC TCATGGTCAA GACTTGGGAA CAGCCGGCAG CTGCCTTCGT 
CGTTTCAGTA CCATGCCTTT CATGTTCTGC AACATCAATA ATGTTTGCAA CTTTGCTTCA 
AGAAATGATT ATTCTTACTG GCTCTCCACC CCAGAGCCCA TGCCAATGAG CATGGAACCC 
CTGAAGGGGC AGAGCATCCA GCCATTCATT AGTCGATGTG CAGTATGTGA AGCTCCGGCC 
GTGGTGATTG CAGTCCACAG TCAGACCATC CAGATTCCCC GTTGTCCTCA GGGATGGGAT 
TCTCTCTGGA TTGGTTATTC CTTCATGATG CACACAAGTG CAGGGGCAGA GGGCTCAGGC 
CAAGCCCTGG CCTCCCCTGG TTCCTGCTTA GAAGAGTTTC GTTCAGCTCC CTTCATTGAG 
TGTCATGGGC GGGGGACCTG CAACTATTAC GCCAACTCCT ACAGCTTTTG GCTGGCGACT 
GTAGATGTGG CAGACATGTT CAGCAAACCT CAATCAGAAA CGCTGAAAGC AGGAGACTTG 
AGGACACGTA TTAGCCGATG TCAAGTGTGC ATGAAGAGGA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006072403.1
CDS	321..5405
Translation	MKLRGVSLAAGLSLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGPAGPPGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGVKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGLPGPPGLVIPRPGTGVTVGEKGNIGLPGLPGDKGDRGFPGIQGPPGLPGPPGPAVIGPPGPPGFPGERGQKGDEGPPGISIPGSPGLDGQPGAPGPPGPPGPPGPHIPPSDKICEAGPPGPPGSPGDRGLQGEQGMKGDKGDTCFNCIGTGVSGPPGQPGLPGLPGPPGSLGFPGQKGEKGHAGATGPKGLTGIPGAPGAPGFPGPKGEPGDILTFPGMKGDKGDLGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGMAFKGERGPPGNPGLPGLPGNRGPMGPVGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGHPGRDGEVGLPGEPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGSPGRIGLEGPSGPPGFPGPKGEPGFGLPGPPGPPGLPGFKGILGPKGDRGFPGPQGPPGQAGLDGLPGPKGDIGPNGQPGPIGPPGLPGTGFQGPPGPPGIPGPIGQPGLPGLHGLPGEKGDPGPPGFDVPGPPGERGSPGIPGAPGPMGPPGSPGLPGKAGASGFPGAKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGLPGPMDPDLLGSKGEKGDPGLPGIPGVAGPKGYQGLPGDPGQPGLSGQPGLPGPAGPKGNPGLPGMPGLTGPPGLKGNMGDMGFPGPQGPKGSFGPPGVPGQPGSPGLPGQKGEKGDPGISGIGLPGLPGPKGEPGLPGYPGNPGTKGSMGETGLPGLPGTPGAKGQPGLPGFPGTPGLPGPKGISGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDEGLPGIPGNPGLPGPKGEPGFHGFPGLQGPPGPPGSPGPALEGPKGSPGPQGPPGRPGPTGFQGPPGPEGPRGLPGSGGIKGERGNPGQPGQPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSTGPEGDPGLIGPPGPPGLPGPSGQSIVIKGDAGPPGIPGQPGLKGLQGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTIQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVADMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_006072341.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen alpha-5(IV) chain-like (LOC102399306), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006072341.1

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG TCTACCAGGC 
TTGCATGGAC TACCAGGAGA GAAGGGGGAT CCAGGGCCTC CTGGATTTGA TGTTCCAGGA 
CCTCCTGGTG AGAGAGGCAG CCCAGGGATC CCTGGAGCAC CAGGTCCTAT GGGACCCCCA 
GGATCACCAG GGCTCCCAGG AAAAGCAGGT GCCTCTGGCT TTCCAGGTGC CAAAGGTGAA 
ATGGGTATGA TGGGACCTCC AGGCCCACCA GGACCTTTGG GAATTCCTGG CAGGAGTGGT 
GTTCCTGGTC TCAAAGGTGA TGATGGTTTG CAGGGTCAGC CAGGACTTCC TGGCCCTGCA 
GGAGAGAAAG GTAGTAAAGG AGAGCCTGGC CTTCCAGGCC TTCCTGGGCC TATGGATCCA 
GATCTGCTGG GCTCAAAAGG AGAGAAAGGG GACCCTGGCC TACCAGGTAT TCCTGGAGTT 
GCAGGGCCAA AAGGTTACCA GGGTTTACCT GGAGACCCAG GGCAACCTGG ACTGAGTGGA 
CAACCTGGAT TGCCAGGACC AGCAGGTCCC AAGGGTAACC CTGGTCTCCC TGGAATGCCA 
GGACTTACAG GACCTCCTGG ACTGAAAGGA AACATGGGTG ATATGGGTTT TCCAGGCCCA 
CAGGGTCCAA AAGGGTCTTT CGGACCTCCT GGAGTTCCTG GACAACCTGG CTCCCCAGGA 
TTACCTGGAC AGAAAGGAGA AAAAGGTGAT CCTGGTATTT CAGGCATTGG TCTTCCAGGT 
CTTCCTGGCC CAAAGGGTGA ACCTGGTCTG CCTGGATACC CAGGGAACCC TGGTACCAAA 
GGTTCTATGG GAGAAACTGG TTTGCCTGGA TTACCGGGGA CCCCTGGAGC AAAAGGACAA 
CCAGGCCTTC CTGGATTCCC TGGAACCCCA GGCCTTCCTG GACCAAAAGG TATTAGTGGT 
CCTCCTGGGA ACCCTGGCCT TCCAGGAGAA CCTGGTCCTG TAGGTGGTGG AGGTCGTCCT 
GGGCCACCAG GGCCTCCAGG TGAAAAAGGC AAACCAGGTC AAGATGGTAT TCCTGGACCA 
GCTGGACAGA AGGGTGAACC AGGTCAACCA GGCTTTGGAA TCCCAGGACC CCCTGGACTT 
CCAGGACTTT CTGGTCAAAA GGGTGATGAA GGATTACCTG GCATTCCAGG AAACCCTGGC 
CTTCCAGGTC CAAAGGGTGA ACCAGGCTTT CATGGTTTCC CTGGTCTGCA GGGTCCCCCA 
GGCCCTCCTG GTTCTCCAGG TCCAGCTCTG GAAGGCCCTA AAGGCAGCCC TGGACCCCAA 
GGTCCTCCTG GGAGACCAGG TCCTACAGGT TTTCAAGGTC CACCAGGTCC AGAAGGTCCC 
CGGGGTCTAC CTGGAAGTGG GGGTATTAAA GGAGAAAGAG GTAACCCAGG CCAACCTGGG 
CAACCTGGTT TGCCTGGTTT GAAAGGTGAT CAAGGTCCTC CAGGACTCCA GGGTAACCCT 
GGCCGGCCAG GTCTCAATGG AATGAAAGGA GATCCTGGTC TCCCTGGTGT TCCAGGATTT 
CCAGGCATGA AAGGACCCAG TGGAGTACCT GGTTCAACTG GCCCTGAGGG GGATCCAGGA 
CTTATTGGCC CACCAGGTCC CCCTGGATTA CCTGGTCCTT CAGGTCAGAG TATTGTAATC 
AAAGGAGATG CTGGCCCACC AGGGATTCCA GGCCAGCCTG GATTAAAAGG TTTACAAGGA 
CTCCCAGGAC CTCAGGGTTT ACCAGGTCCA ATTGGCCCTC CAGGAGATCC TGGACGCAAT 
GGACTCCCTG GCTTTGATGG TGCAGGAGGA CGCAAAGGAG ACCCAGGTTT GCCAGGCCAG 
CCAGGTACTC GTGGCTTGGA TGGTCCCCCC GGGCCAGATG GATTGCAGGG TCCTCCAGGT 
CCCCCTGGAA CCTCTTCTAT CGCCCATGGG TTTCTCATCA CACGTCACAG CCAGACAACA 
GATGCTCCAC AATGCCCACA GGGAACCATC CAGGTGTATG AAGGCTTTTC TCTCCTGTAT 
GTACAAGGAA ATAAAAGAGC TCATGGTCAA GACTTGGGAA CAGCCGGCAG CTGCCTTCGT 
CGTTTCAGTA CCATGCCTTT CATGTTCTGC AACATCAATA ATGTTTGCAA CTTTGCTTCA 
AGAAATGATT ATTCTTACTG GCTCTCCACC CCAGAGCCCA TGCCAATGAG CATGGAACCC 
CTGAAGGGGC AGAGCATCCA GCCATTCATT AGTCGATGTG CAGTATGTGA AGCTCCGGCC 
GTGGTGATTG CAGTCCACAG TCAGACCATC CAGATTCCCC GTTGTCCTCA GGGATGGGAT 
TCTCTCTGGA TTGGTTATTC CTTCATGATG CACACAAGTG CAGGGGCAGA GGGCTCAGGC 
CAAGCCCTGG CCTCCCCTGG TTCCTGCTTA GAAGAGTTTC GTTCAGCTCC CTTCATTGAG 
TGTCATGGGC GGGGGACCTG CAACTATTAC GCCAACTCCT ACAGCTTTTG GCTGGCGACT 
GTAGATGTGG CAGACATGTT CAGCAAACCT CAATCAGAAA CGCTGAAAGC AGGAGACTTG 
AGGACACGTA TTAGCCGATG TCAAGTGTGC ATGAAGAGGA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102399306 ( XM_006072341.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006072403.1 Bubalus bubalis collagen type IV alpha 5 chain (COL4A5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu26601
Clone ID Related Accession (Same CDS sequence)	XM_006072341.1 , XM_006072341.2
Accession Version	XM_006072341.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-5(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006072341.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG TCTACCAGGC 
TTGCATGGAC TACCAGGAGA GAAGGGGGAT CCAGGGCCTC CTGGATTTGA TGTTCCAGGA 
CCTCCTGGTG AGAGAGGCAG CCCAGGGATC CCTGGAGCAC CAGGTCCTAT GGGACCCCCA 
GGATCACCAG GGCTCCCAGG AAAAGCAGGT GCCTCTGGCT TTCCAGGTGC CAAAGGTGAA 
ATGGGTATGA TGGGACCTCC AGGCCCACCA GGACCTTTGG GAATTCCTGG CAGGAGTGGT 
GTTCCTGGTC TCAAAGGTGA TGATGGTTTG CAGGGTCAGC CAGGACTTCC TGGCCCTGCA 
GGAGAGAAAG GTAGTAAAGG AGAGCCTGGC CTTCCAGGCC TTCCTGGGCC TATGGATCCA 
GATCTGCTGG GCTCAAAAGG AGAGAAAGGG GACCCTGGCC TACCAGGTAT TCCTGGAGTT 
GCAGGGCCAA AAGGTTACCA GGGTTTACCT GGAGACCCAG GGCAACCTGG ACTGAGTGGA 
CAACCTGGAT TGCCAGGACC AGCAGGTCCC AAGGGTAACC CTGGTCTCCC TGGAATGCCA 
GGACTTACAG GACCTCCTGG ACTGAAAGGA AACATGGGTG ATATGGGTTT TCCAGGCCCA 
CAGGGTCCAA AAGGGTCTTT CGGACCTCCT GGAGTTCCTG GACAACCTGG CTCCCCAGGA 
TTACCTGGAC AGAAAGGAGA AAAAGGTGAT CCTGGTATTT CAGGCATTGG TCTTCCAGGT 
CTTCCTGGCC CAAAGGGTGA ACCTGGTCTG CCTGGATACC CAGGGAACCC TGGTACCAAA 
GGTTCTATGG GAGAAACTGG TTTGCCTGGA TTACCGGGGA CCCCTGGAGC AAAAGGACAA 
CCAGGCCTTC CTGGATTCCC TGGAACCCCA GGCCTTCCTG GACCAAAAGG TATTAGTGGT 
CCTCCTGGGA ACCCTGGCCT TCCAGGAGAA CCTGGTCCTG TAGGTGGTGG AGGTCGTCCT 
GGGCCACCAG GGCCTCCAGG TGAAAAAGGC AAACCAGGTC AAGATGGTAT TCCTGGACCA 
GCTGGACAGA AGGGTGAACC AGGTCAACCA GGCTTTGGAA TCCCAGGACC CCCTGGACTT 
CCAGGACTTT CTGGTCAAAA GGGTGATGAA GGATTACCTG GCATTCCAGG AAACCCTGGC 
CTTCCAGGTC CAAAGGGTGA ACCAGGCTTT CATGGTTTCC CTGGTCTGCA GGGTCCCCCA 
GGCCCTCCTG GTTCTCCAGG TCCAGCTCTG GAAGGCCCTA AAGGCAGCCC TGGACCCCAA 
GGTCCTCCTG GGAGACCAGG TCCTACAGGT TTTCAAGGTC CACCAGGTCC AGAAGGTCCC 
CGGGGTCTAC CTGGAAGTGG GGGTATTAAA GGAGAAAGAG GTAACCCAGG CCAACCTGGG 
CAACCTGGTT TGCCTGGTTT GAAAGGTGAT CAAGGTCCTC CAGGACTCCA GGGTAACCCT 
GGCCGGCCAG GTCTCAATGG AATGAAAGGA GATCCTGGTC TCCCTGGTGT TCCAGGATTT 
CCAGGCATGA AAGGACCCAG TGGAGTACCT GGTTCAACTG GCCCTGAGGG GGATCCAGGA 
CTTATTGGCC CACCAGGTCC CCCTGGATTA CCTGGTCCTT CAGGTCAGAG TATTGTAATC 
AAAGGAGATG CTGGCCCACC AGGGATTCCA GGCCAGCCTG GATTAAAAGG TTTACAAGGA 
CTCCCAGGAC CTCAGGGTTT ACCAGGTCCA ATTGGCCCTC CAGGAGATCC TGGACGCAAT 
GGACTCCCTG GCTTTGATGG TGCAGGAGGA CGCAAAGGAG ACCCAGGTTT GCCAGGCCAG 
CCAGGTACTC GTGGCTTGGA TGGTCCCCCC GGGCCAGATG GATTGCAGGG TCCTCCAGGT 
CCCCCTGGAA CCTCTTCTAT CGCCCATGGG TTTCTCATCA CACGTCACAG CCAGACAACA 
GATGCTCCAC AATGCCCACA GGGAACCATC CAGGTGTATG AAGGCTTTTC TCTCCTGTAT 
GTACAAGGAA ATAAAAGAGC TCATGGTCAA GACTTGGGAA CAGCCGGCAG CTGCCTTCGT 
CGTTTCAGTA CCATGCCTTT CATGTTCTGC AACATCAATA ATGTTTGCAA CTTTGCTTCA 
AGAAATGATT ATTCTTACTG GCTCTCCACC CCAGAGCCCA TGCCAATGAG CATGGAACCC 
CTGAAGGGGC AGAGCATCCA GCCATTCATT AGTCGATGTG CAGTATGTGA AGCTCCGGCC 
GTGGTGATTG CAGTCCACAG TCAGACCATC CAGATTCCCC GTTGTCCTCA GGGATGGGAT 
TCTCTCTGGA TTGGTTATTC CTTCATGATG CACACAAGTG CAGGGGCAGA GGGCTCAGGC 
CAAGCCCTGG CCTCCCCTGG TTCCTGCTTA GAAGAGTTTC GTTCAGCTCC CTTCATTGAG 
TGTCATGGGC GGGGGACCTG CAACTATTAC GCCAACTCCT ACAGCTTTTG GCTGGCGACT 
GTAGATGTGG CAGACATGTT CAGCAAACCT CAATCAGAAA CGCTGAAAGC AGGAGACTTG 
AGGACACGTA TTAGCCGATG TCAAGTGTGC ATGAAGAGGA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006072403.1
CDS	328..5412
Translation	MKLRGVSLAAGLSLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGPAGPPGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGVKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGLPGPPGLVIPRPGTGVTVGEKGNIGLPGLPGDKGDRGFPGIQGPPGLPGPPGPAVIGPPGPPGFPGERGQKGDEGPPGISIPGSPGLDGQPGAPGPPGPPGPPGPHIPPSDKICEAGPPGPPGSPGDRGLQGEQGMKGDKGDTCFNCIGTGVSGPPGQPGLPGLPGPPGSLGFPGQKGEKGHAGATGPKGLTGIPGAPGAPGFPGPKGEPGDILTFPGMKGDKGDLGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGMAFKGERGPPGNPGLPGLPGNRGPMGPVGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGHPGRDGEVGLPGEPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGSPGRIGLEGPSGPPGFPGPKGEPGFGLPGPPGPPGLPGFKGILGPKGDRGFPGPQGPPGQAGLDGLPGPKGDIGPNGQPGPIGPPGLPGTGFQGPPGPPGIPGPIGQPGLPGLHGLPGEKGDPGPPGFDVPGPPGERGSPGIPGAPGPMGPPGSPGLPGKAGASGFPGAKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGLPGPMDPDLLGSKGEKGDPGLPGIPGVAGPKGYQGLPGDPGQPGLSGQPGLPGPAGPKGNPGLPGMPGLTGPPGLKGNMGDMGFPGPQGPKGSFGPPGVPGQPGSPGLPGQKGEKGDPGISGIGLPGLPGPKGEPGLPGYPGNPGTKGSMGETGLPGLPGTPGAKGQPGLPGFPGTPGLPGPKGISGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDEGLPGIPGNPGLPGPKGEPGFHGFPGLQGPPGPPGSPGPALEGPKGSPGPQGPPGRPGPTGFQGPPGPEGPRGLPGSGGIKGERGNPGQPGQPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSTGPEGDPGLIGPPGPPGLPGPSGQSIVIKGDAGPPGIPGQPGLKGLQGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTIQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVADMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_006072341.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen type IV alpha 5 chain (COL4A5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006072341.2

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG TCTACCAGGC 
TTGCATGGAC TACCAGGAGA GAAGGGGGAT CCAGGGCCTC CTGGATTTGA TGTTCCAGGA 
CCTCCTGGTG AGAGAGGCAG CCCAGGGATC CCTGGAGCAC CAGGTCCTAT GGGACCCCCA 
GGATCACCAG GGCTCCCAGG AAAAGCAGGT GCCTCTGGCT TTCCAGGTGC CAAAGGTGAA 
ATGGGTATGA TGGGACCTCC AGGCCCACCA GGACCTTTGG GAATTCCTGG CAGGAGTGGT 
GTTCCTGGTC TCAAAGGTGA TGATGGTTTG CAGGGTCAGC CAGGACTTCC TGGCCCTGCA 
GGAGAGAAAG GTAGTAAAGG AGAGCCTGGC CTTCCAGGCC TTCCTGGGCC TATGGATCCA 
GATCTGCTGG GCTCAAAAGG AGAGAAAGGG GACCCTGGCC TACCAGGTAT TCCTGGAGTT 
GCAGGGCCAA AAGGTTACCA GGGTTTACCT GGAGACCCAG GGCAACCTGG ACTGAGTGGA 
CAACCTGGAT TGCCAGGACC AGCAGGTCCC AAGGGTAACC CTGGTCTCCC TGGAATGCCA 
GGACTTACAG GACCTCCTGG ACTGAAAGGA AACATGGGTG ATATGGGTTT TCCAGGCCCA 
CAGGGTCCAA AAGGGTCTTT CGGACCTCCT GGAGTTCCTG GACAACCTGG CTCCCCAGGA 
TTACCTGGAC AGAAAGGAGA AAAAGGTGAT CCTGGTATTT CAGGCATTGG TCTTCCAGGT 
CTTCCTGGCC CAAAGGGTGA ACCTGGTCTG CCTGGATACC CAGGGAACCC TGGTACCAAA 
GGTTCTATGG GAGAAACTGG TTTGCCTGGA TTACCGGGGA CCCCTGGAGC AAAAGGACAA 
CCAGGCCTTC CTGGATTCCC TGGAACCCCA GGCCTTCCTG GACCAAAAGG TATTAGTGGT 
CCTCCTGGGA ACCCTGGCCT TCCAGGAGAA CCTGGTCCTG TAGGTGGTGG AGGTCGTCCT 
GGGCCACCAG GGCCTCCAGG TGAAAAAGGC AAACCAGGTC AAGATGGTAT TCCTGGACCA 
GCTGGACAGA AGGGTGAACC AGGTCAACCA GGCTTTGGAA TCCCAGGACC CCCTGGACTT 
CCAGGACTTT CTGGTCAAAA GGGTGATGAA GGATTACCTG GCATTCCAGG AAACCCTGGC 
CTTCCAGGTC CAAAGGGTGA ACCAGGCTTT CATGGTTTCC CTGGTCTGCA GGGTCCCCCA 
GGCCCTCCTG GTTCTCCAGG TCCAGCTCTG GAAGGCCCTA AAGGCAGCCC TGGACCCCAA 
GGTCCTCCTG GGAGACCAGG TCCTACAGGT TTTCAAGGTC CACCAGGTCC AGAAGGTCCC 
CGGGGTCTAC CTGGAAGTGG GGGTATTAAA GGAGAAAGAG GTAACCCAGG CCAACCTGGG 
CAACCTGGTT TGCCTGGTTT GAAAGGTGAT CAAGGTCCTC CAGGACTCCA GGGTAACCCT 
GGCCGGCCAG GTCTCAATGG AATGAAAGGA GATCCTGGTC TCCCTGGTGT TCCAGGATTT 
CCAGGCATGA AAGGACCCAG TGGAGTACCT GGTTCAACTG GCCCTGAGGG GGATCCAGGA 
CTTATTGGCC CACCAGGTCC CCCTGGATTA CCTGGTCCTT CAGGTCAGAG TATTGTAATC 
AAAGGAGATG CTGGCCCACC AGGGATTCCA GGCCAGCCTG GATTAAAAGG TTTACAAGGA 
CTCCCAGGAC CTCAGGGTTT ACCAGGTCCA ATTGGCCCTC CAGGAGATCC TGGACGCAAT 
GGACTCCCTG GCTTTGATGG TGCAGGAGGA CGCAAAGGAG ACCCAGGTTT GCCAGGCCAG 
CCAGGTACTC GTGGCTTGGA TGGTCCCCCC GGGCCAGATG GATTGCAGGG TCCTCCAGGT 
CCCCCTGGAA CCTCTTCTAT CGCCCATGGG TTTCTCATCA CACGTCACAG CCAGACAACA 
GATGCTCCAC AATGCCCACA GGGAACCATC CAGGTGTATG AAGGCTTTTC TCTCCTGTAT 
GTACAAGGAA ATAAAAGAGC TCATGGTCAA GACTTGGGAA CAGCCGGCAG CTGCCTTCGT 
CGTTTCAGTA CCATGCCTTT CATGTTCTGC AACATCAATA ATGTTTGCAA CTTTGCTTCA 
AGAAATGATT ATTCTTACTG GCTCTCCACC CCAGAGCCCA TGCCAATGAG CATGGAACCC 
CTGAAGGGGC AGAGCATCCA GCCATTCATT AGTCGATGTG CAGTATGTGA AGCTCCGGCC 
GTGGTGATTG CAGTCCACAG TCAGACCATC CAGATTCCCC GTTGTCCTCA GGGATGGGAT 
TCTCTCTGGA TTGGTTATTC CTTCATGATG CACACAAGTG CAGGGGCAGA GGGCTCAGGC 
CAAGCCCTGG CCTCCCCTGG TTCCTGCTTA GAAGAGTTTC GTTCAGCTCC CTTCATTGAG 
TGTCATGGGC GGGGGACCTG CAACTATTAC GCCAACTCCT ACAGCTTTTG GCTGGCGACT 
GTAGATGTGG CAGACATGTT CAGCAAACCT CAATCAGAAA CGCTGAAAGC AGGAGACTTG 
AGGACACGTA TTAGCCGATG TCAAGTGTGC ATGAAGAGGA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102399306 ( XM_006072342.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006072404.1 Bubalus bubalis collagen alpha-5(IV) chain-like (LOC102399306), transcript variant X2, mRNA.

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CloneID	OBu26602
Clone ID Related Accession (Same CDS sequence)	XM_006072342.1 , XM_006072342.2
Accession Version	XM_006072342.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-5(IV) chain-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785484.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTCCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CTATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGC CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC CAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAACCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA 
CCTGGAAGTG GGGGTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT 
TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC 
CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGTCAGA GTATTGTAAT CAAAGGAGAT 
GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA 
CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT 
CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA 
ACCTCTTCTA TCGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA 
CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT 
TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT 
GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG 
ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG 
GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG 
CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG 
GCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006072404.1
CDS	321..5396
Translation	MKLRGVSLAAGLSLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGPAGPPGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGVKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGLPGPPGLVIPRPGTGVTVGEKGNIGLPGLPGDKGDRGFPGIQGPPGLPGPPGPAVIGPPGPPGFPGERGQKGDEGPPGISIPGSPGLDGQPGAPGPPGPPGPPGPHIPPSDKICEAGPPGPPGSPGDRGLQGEQGMKGDKGDTCFNCIGTGVSGPPGQPGLPGLPGPPGSLGFPGQKGEKGHAGATGPKGLTGIPGAPGAPGFPGPKGEPGDILTFPGMKGDKGDLGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGMAFKGERGPPGNPGLPGLPGNRGPMGPVGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGHPGRDGEVGLPGEPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGSPGRIGLEGPSGPPGFPGPKGEPGFGLPGPPGPPGLPGFKGILGPKGDRGFPGPQGPPGQAGLDGLPGPKGDIGPNGQPGPIGPPGLPGTGFQGPPGPPGIPGPIGQPGLHGLPGEKGDPGPPGFDVPGPPGERGSPGIPGAPGPMGPPGSPGLPGKAGASGFPGAKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGLPGPMDPDLLGSKGEKGDPGLPGIPGVAGPKGYQGLPGDPGQPGLSGQPGLPGPAGPKGNPGLPGMPGLTGPPGLKGNMGDMGFPGPQGPKGSFGPPGVPGQPGSPGLPGQKGEKGDPGISGIGLPGLPGPKGEPGLPGYPGNPGTKGSMGETGLPGLPGTPGAKGQPGLPGFPGTPGLPGPKGISGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDEGLPGIPGNPGLPGPKGEPGFHGFPGLQGPPGPPGSPGPALEGPKGSPGPQGPPGRPGPTGFQGPPGPEGPRGLPGSGGIKGERGNPGQPGQPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSTGPEGDPGLIGPPGPPGLPGPSGQSIVIKGDAGPPGIPGQPGLKGLQGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTIQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVADMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_006072342.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen alpha-5(IV) chain-like (LOC102399306), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006072342.1

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTCCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CTATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGC CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC CAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAACCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA 
CCTGGAAGTG GGGGTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT 
TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC 
CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGTCAGA GTATTGTAAT CAAAGGAGAT 
GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA 
CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT 
CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA 
ACCTCTTCTA TCGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA 
CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT 
TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT 
GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG 
ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG 
GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG 
CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG 
GCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102399306 ( XM_006072342.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006072404.1 Bubalus bubalis collagen type IV alpha 5 chain (COL4A5), transcript variant X2, mRNA.

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CloneID	OBu26602
Clone ID Related Accession (Same CDS sequence)	XM_006072342.1 , XM_006072342.2
Accession Version	XM_006072342.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-5(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006072342.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTCCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CTATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGC CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC CAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAACCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA 
CCTGGAAGTG GGGGTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT 
TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC 
CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGTCAGA GTATTGTAAT CAAAGGAGAT 
GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA 
CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT 
CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA 
ACCTCTTCTA TCGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA 
CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT 
TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT 
GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG 
ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG 
GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG 
CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG 
GCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006072404.1
CDS	328..5403
Translation	MKLRGVSLAAGLSLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGPAGPPGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGVKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGLPGPPGLVIPRPGTGVTVGEKGNIGLPGLPGDKGDRGFPGIQGPPGLPGPPGPAVIGPPGPPGFPGERGQKGDEGPPGISIPGSPGLDGQPGAPGPPGPPGPPGPHIPPSDKICEAGPPGPPGSPGDRGLQGEQGMKGDKGDTCFNCIGTGVSGPPGQPGLPGLPGPPGSLGFPGQKGEKGHAGATGPKGLTGIPGAPGAPGFPGPKGEPGDILTFPGMKGDKGDLGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGMAFKGERGPPGNPGLPGLPGNRGPMGPVGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGHPGRDGEVGLPGEPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGSPGRIGLEGPSGPPGFPGPKGEPGFGLPGPPGPPGLPGFKGILGPKGDRGFPGPQGPPGQAGLDGLPGPKGDIGPNGQPGPIGPPGLPGTGFQGPPGPPGIPGPIGQPGLHGLPGEKGDPGPPGFDVPGPPGERGSPGIPGAPGPMGPPGSPGLPGKAGASGFPGAKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGLPGPMDPDLLGSKGEKGDPGLPGIPGVAGPKGYQGLPGDPGQPGLSGQPGLPGPAGPKGNPGLPGMPGLTGPPGLKGNMGDMGFPGPQGPKGSFGPPGVPGQPGSPGLPGQKGEKGDPGISGIGLPGLPGPKGEPGLPGYPGNPGTKGSMGETGLPGLPGTPGAKGQPGLPGFPGTPGLPGPKGISGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDEGLPGIPGNPGLPGPKGEPGFHGFPGLQGPPGPPGSPGPALEGPKGSPGPQGPPGRPGPTGFQGPPGPEGPRGLPGSGGIKGERGNPGQPGQPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSTGPEGDPGLIGPPGPPGLPGPSGQSIVIKGDAGPPGIPGQPGLKGLQGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTIQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVADMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_006072342.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen type IV alpha 5 chain (COL4A5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006072342.2

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTCCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CTATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGC CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC CAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAACCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA 
CCTGGAAGTG GGGGTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT 
TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC 
CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGTCAGA GTATTGTAAT CAAAGGAGAT 
GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA 
CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT 
CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA 
ACCTCTTCTA TCGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA 
CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT 
TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT 
GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG 
ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG 
GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG 
CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG 
GCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102399306 ( XM_006072343.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006072405.1 Bubalus bubalis collagen type IV alpha 5 chain (COL4A5), transcript variant X3, mRNA.

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CloneID	OBu26603
Clone ID Related Accession (Same CDS sequence)	XM_006072343.2 , XM_006072343.1
Accession Version	XM_006072343.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-5(IV) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006072343.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG TCTACCAGGC 
TTGCATGGAC TACCAGGAGA GAAGGGGGAT CCAGGGCCTC CTGGATTTGA TGTTCCAGGA 
CCTCCTGGTG AGAGAGGCAG CCCAGGGATC CCTGGAGCAC CAGGTCCTAT GGGACCCCCA 
GGATCACCAG GGCTCCCAGG AAAAGCAGGT GCCTCTGGCT TTCCAGGTGC CAAAGGTGAA 
ATGGGTATGA TGGGACCTCC AGGCCCACCA GGACCTTTGG GAATTCCTGG CAGGAGTGGT 
GTTCCTGGTC TCAAAGGTGA TGATGGTTTG CAGGGTCAGC CAGGACTTCC TGGCCCTGCA 
GGAGAGAAAG GTAGTAAAGG AGAGCCTGGC CTTCCAGGCC TTCCTGGGCC TATGGATCCA 
GATCTGCTGG GCTCAAAAGG AGAGAAAGGG GACCCTGGCC TACCAGGTAT TCCTGGAGTT 
GCAGGGCCAA AAGGTTACCA GGGTTTACCT GGAGACCCAG GGCAACCTGG ACTGAGTGGA 
CAACCTGGAT TGCCAGGACC AGCAGGTCCC AAGGGTAACC CTGGTCTCCC TGGAATGCCA 
GGACTTACAG GACCTCCTGG ACTGAAAGGA AACATGGGTG ATATGGGTTT TCCAGGCCCA 
CAGGGTCCAA AAGGGTCTTT CGGACCTCCT GGAGTTCCTG GACAACCTGG CTCCCCAGGA 
TTACCTGGAC AGAAAGGAGA AAAAGGTGAT CCTGGTATTT CAGGCATTGG TCTTCCAGGT 
CTTCCTGGCC CAAAGGGTGA ACCTGGTCTG CCTGGATACC CAGGGAACCC TGGTACCAAA 
GGTTCTATGG GAGAAACTGG TTTGCCTGGA TTACCGGGGA CCCCTGGAGC AAAAGGACAA 
CCAGGCCTTC CTGGATTCCC TGGAACCCCA GGCCTTCCTG GACCAAAAGG TATTAGTGGT 
CCTCCTGGGA ACCCTGGCCT TCCAGGAGAA CCTGGTCCTG TAGGTGGTGG AGGTCGTCCT 
GGGCCACCAG GGCCTCCAGG TGAAAAAGGC AAACCAGGTC AAGATGGTAT TCCTGGACCA 
GCTGGACAGA AGGGTGAACC AGGTCAACCA GGCTTTGGAA TCCCAGGACC CCCTGGACTT 
CCAGGACTTT CTGGTCAAAA GGGTGATGAA GGATTACCTG GCATTCCAGG AAACCCTGGC 
CTTCCAGGTC CAAAGGGTGA ACCAGGCTTT CATGGTTTCC CTGGTCTGCA GGGTCCCCCA 
GGCCCTCCTG GTTCTCCAGG TCCAGCTCTG GAAGGCCCTA AAGGCAGCCC TGGACCCCAA 
GGTCCTCCTG GGAGACCAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA 
CCTGGAAGTG GGGGTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT 
TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC 
CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGTCAGA GTATTGTAAT CAAAGGAGAT 
GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA 
CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT 
CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA 
ACCTCTTCTA TCGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA 
CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT 
TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT 
GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG 
ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG 
GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG 
CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG 
GCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006072405.1
CDS	328..5403
Translation	MKLRGVSLAAGLSLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGPAGPPGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGVKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGLPGPPGLVIPRPGTGVTVGEKGNIGLPGLPGDKGDRGFPGIQGPPGLPGPPGPAVIGPPGPPGFPGERGQKGDEGPPGISIPGSPGLDGQPGAPGPPGPPGPPGPHIPPSDKICEAGPPGPPGSPGDRGLQGEQGMKGDKGDTCFNCIGTGVSGPPGQPGLPGLPGPPGSLGFPGQKGEKGHAGATGPKGLTGIPGAPGAPGFPGPKGEPGDILTFPGMKGDKGDLGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGMAFKGERGPPGNPGLPGLPGNRGPMGPVGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGHPGRDGEVGLPGEPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGSPGRIGLEGPSGPPGFPGPKGEPGFGLPGPPGPPGLPGFKGILGPKGDRGFPGPQGPPGQAGLDGLPGPKGDIGPNGQPGPIGPPGLPGTGFQGPPGPPGIPGPIGQPGLPGLHGLPGEKGDPGPPGFDVPGPPGERGSPGIPGAPGPMGPPGSPGLPGKAGASGFPGAKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGLPGPMDPDLLGSKGEKGDPGLPGIPGVAGPKGYQGLPGDPGQPGLSGQPGLPGPAGPKGNPGLPGMPGLTGPPGLKGNMGDMGFPGPQGPKGSFGPPGVPGQPGSPGLPGQKGEKGDPGISGIGLPGLPGPKGEPGLPGYPGNPGTKGSMGETGLPGLPGTPGAKGQPGLPGFPGTPGLPGPKGISGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDEGLPGIPGNPGLPGPKGEPGFHGFPGLQGPPGPPGSPGPALEGPKGSPGPQGPPGRPGFQGPPGPEGPRGLPGSGGIKGERGNPGQPGQPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSTGPEGDPGLIGPPGPPGLPGPSGQSIVIKGDAGPPGIPGQPGLKGLQGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTIQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVADMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_006072343.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen type IV alpha 5 chain (COL4A5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006072343.2

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG TCTACCAGGC 
TTGCATGGAC TACCAGGAGA GAAGGGGGAT CCAGGGCCTC CTGGATTTGA TGTTCCAGGA 
CCTCCTGGTG AGAGAGGCAG CCCAGGGATC CCTGGAGCAC CAGGTCCTAT GGGACCCCCA 
GGATCACCAG GGCTCCCAGG AAAAGCAGGT GCCTCTGGCT TTCCAGGTGC CAAAGGTGAA 
ATGGGTATGA TGGGACCTCC AGGCCCACCA GGACCTTTGG GAATTCCTGG CAGGAGTGGT 
GTTCCTGGTC TCAAAGGTGA TGATGGTTTG CAGGGTCAGC CAGGACTTCC TGGCCCTGCA 
GGAGAGAAAG GTAGTAAAGG AGAGCCTGGC CTTCCAGGCC TTCCTGGGCC TATGGATCCA 
GATCTGCTGG GCTCAAAAGG AGAGAAAGGG GACCCTGGCC TACCAGGTAT TCCTGGAGTT 
GCAGGGCCAA AAGGTTACCA GGGTTTACCT GGAGACCCAG GGCAACCTGG ACTGAGTGGA 
CAACCTGGAT TGCCAGGACC AGCAGGTCCC AAGGGTAACC CTGGTCTCCC TGGAATGCCA 
GGACTTACAG GACCTCCTGG ACTGAAAGGA AACATGGGTG ATATGGGTTT TCCAGGCCCA 
CAGGGTCCAA AAGGGTCTTT CGGACCTCCT GGAGTTCCTG GACAACCTGG CTCCCCAGGA 
TTACCTGGAC AGAAAGGAGA AAAAGGTGAT CCTGGTATTT CAGGCATTGG TCTTCCAGGT 
CTTCCTGGCC CAAAGGGTGA ACCTGGTCTG CCTGGATACC CAGGGAACCC TGGTACCAAA 
GGTTCTATGG GAGAAACTGG TTTGCCTGGA TTACCGGGGA CCCCTGGAGC AAAAGGACAA 
CCAGGCCTTC CTGGATTCCC TGGAACCCCA GGCCTTCCTG GACCAAAAGG TATTAGTGGT 
CCTCCTGGGA ACCCTGGCCT TCCAGGAGAA CCTGGTCCTG TAGGTGGTGG AGGTCGTCCT 
GGGCCACCAG GGCCTCCAGG TGAAAAAGGC AAACCAGGTC AAGATGGTAT TCCTGGACCA 
GCTGGACAGA AGGGTGAACC AGGTCAACCA GGCTTTGGAA TCCCAGGACC CCCTGGACTT 
CCAGGACTTT CTGGTCAAAA GGGTGATGAA GGATTACCTG GCATTCCAGG AAACCCTGGC 
CTTCCAGGTC CAAAGGGTGA ACCAGGCTTT CATGGTTTCC CTGGTCTGCA GGGTCCCCCA 
GGCCCTCCTG GTTCTCCAGG TCCAGCTCTG GAAGGCCCTA AAGGCAGCCC TGGACCCCAA 
GGTCCTCCTG GGAGACCAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA 
CCTGGAAGTG GGGGTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT 
TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC 
CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGTCAGA GTATTGTAAT CAAAGGAGAT 
GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA 
CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT 
CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA 
ACCTCTTCTA TCGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA 
CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT 
TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT 
GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG 
ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG 
GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG 
CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG 
GCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102399306 ( XM_006072343.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006072405.1 Bubalus bubalis collagen alpha-5(IV) chain-like (LOC102399306), transcript variant X3, mRNA.

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CloneID	OBu26603
Clone ID Related Accession (Same CDS sequence)	XM_006072343.2 , XM_006072343.1
Accession Version	XM_006072343.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-5(IV) chain-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785484.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG TCTACCAGGC 
TTGCATGGAC TACCAGGAGA GAAGGGGGAT CCAGGGCCTC CTGGATTTGA TGTTCCAGGA 
CCTCCTGGTG AGAGAGGCAG CCCAGGGATC CCTGGAGCAC CAGGTCCTAT GGGACCCCCA 
GGATCACCAG GGCTCCCAGG AAAAGCAGGT GCCTCTGGCT TTCCAGGTGC CAAAGGTGAA 
ATGGGTATGA TGGGACCTCC AGGCCCACCA GGACCTTTGG GAATTCCTGG CAGGAGTGGT 
GTTCCTGGTC TCAAAGGTGA TGATGGTTTG CAGGGTCAGC CAGGACTTCC TGGCCCTGCA 
GGAGAGAAAG GTAGTAAAGG AGAGCCTGGC CTTCCAGGCC TTCCTGGGCC TATGGATCCA 
GATCTGCTGG GCTCAAAAGG AGAGAAAGGG GACCCTGGCC TACCAGGTAT TCCTGGAGTT 
GCAGGGCCAA AAGGTTACCA GGGTTTACCT GGAGACCCAG GGCAACCTGG ACTGAGTGGA 
CAACCTGGAT TGCCAGGACC AGCAGGTCCC AAGGGTAACC CTGGTCTCCC TGGAATGCCA 
GGACTTACAG GACCTCCTGG ACTGAAAGGA AACATGGGTG ATATGGGTTT TCCAGGCCCA 
CAGGGTCCAA AAGGGTCTTT CGGACCTCCT GGAGTTCCTG GACAACCTGG CTCCCCAGGA 
TTACCTGGAC AGAAAGGAGA AAAAGGTGAT CCTGGTATTT CAGGCATTGG TCTTCCAGGT 
CTTCCTGGCC CAAAGGGTGA ACCTGGTCTG CCTGGATACC CAGGGAACCC TGGTACCAAA 
GGTTCTATGG GAGAAACTGG TTTGCCTGGA TTACCGGGGA CCCCTGGAGC AAAAGGACAA 
CCAGGCCTTC CTGGATTCCC TGGAACCCCA GGCCTTCCTG GACCAAAAGG TATTAGTGGT 
CCTCCTGGGA ACCCTGGCCT TCCAGGAGAA CCTGGTCCTG TAGGTGGTGG AGGTCGTCCT 
GGGCCACCAG GGCCTCCAGG TGAAAAAGGC AAACCAGGTC AAGATGGTAT TCCTGGACCA 
GCTGGACAGA AGGGTGAACC AGGTCAACCA GGCTTTGGAA TCCCAGGACC CCCTGGACTT 
CCAGGACTTT CTGGTCAAAA GGGTGATGAA GGATTACCTG GCATTCCAGG AAACCCTGGC 
CTTCCAGGTC CAAAGGGTGA ACCAGGCTTT CATGGTTTCC CTGGTCTGCA GGGTCCCCCA 
GGCCCTCCTG GTTCTCCAGG TCCAGCTCTG GAAGGCCCTA AAGGCAGCCC TGGACCCCAA 
GGTCCTCCTG GGAGACCAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA 
CCTGGAAGTG GGGGTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT 
TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC 
CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGTCAGA GTATTGTAAT CAAAGGAGAT 
GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA 
CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT 
CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA 
ACCTCTTCTA TCGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA 
CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT 
TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT 
GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG 
ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG 
GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG 
CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG 
GCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006072405.1
CDS	321..5396
Translation	MKLRGVSLAAGLSLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGPAGPPGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGVKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGLPGPPGLVIPRPGTGVTVGEKGNIGLPGLPGDKGDRGFPGIQGPPGLPGPPGPAVIGPPGPPGFPGERGQKGDEGPPGISIPGSPGLDGQPGAPGPPGPPGPPGPHIPPSDKICEAGPPGPPGSPGDRGLQGEQGMKGDKGDTCFNCIGTGVSGPPGQPGLPGLPGPPGSLGFPGQKGEKGHAGATGPKGLTGIPGAPGAPGFPGPKGEPGDILTFPGMKGDKGDLGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGMAFKGERGPPGNPGLPGLPGNRGPMGPVGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGHPGRDGEVGLPGEPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGSPGRIGLEGPSGPPGFPGPKGEPGFGLPGPPGPPGLPGFKGILGPKGDRGFPGPQGPPGQAGLDGLPGPKGDIGPNGQPGPIGPPGLPGTGFQGPPGPPGIPGPIGQPGLPGLHGLPGEKGDPGPPGFDVPGPPGERGSPGIPGAPGPMGPPGSPGLPGKAGASGFPGAKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGLPGPMDPDLLGSKGEKGDPGLPGIPGVAGPKGYQGLPGDPGQPGLSGQPGLPGPAGPKGNPGLPGMPGLTGPPGLKGNMGDMGFPGPQGPKGSFGPPGVPGQPGSPGLPGQKGEKGDPGISGIGLPGLPGPKGEPGLPGYPGNPGTKGSMGETGLPGLPGTPGAKGQPGLPGFPGTPGLPGPKGISGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDEGLPGIPGNPGLPGPKGEPGFHGFPGLQGPPGPPGSPGPALEGPKGSPGPQGPPGRPGFQGPPGPEGPRGLPGSGGIKGERGNPGQPGQPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSTGPEGDPGLIGPPGPPGLPGPSGQSIVIKGDAGPPGIPGQPGLKGLQGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTIQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVADMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_006072343.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen alpha-5(IV) chain-like (LOC102399306), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006072343.1

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG TCTACCAGGC 
TTGCATGGAC TACCAGGAGA GAAGGGGGAT CCAGGGCCTC CTGGATTTGA TGTTCCAGGA 
CCTCCTGGTG AGAGAGGCAG CCCAGGGATC CCTGGAGCAC CAGGTCCTAT GGGACCCCCA 
GGATCACCAG GGCTCCCAGG AAAAGCAGGT GCCTCTGGCT TTCCAGGTGC CAAAGGTGAA 
ATGGGTATGA TGGGACCTCC AGGCCCACCA GGACCTTTGG GAATTCCTGG CAGGAGTGGT 
GTTCCTGGTC TCAAAGGTGA TGATGGTTTG CAGGGTCAGC CAGGACTTCC TGGCCCTGCA 
GGAGAGAAAG GTAGTAAAGG AGAGCCTGGC CTTCCAGGCC TTCCTGGGCC TATGGATCCA 
GATCTGCTGG GCTCAAAAGG AGAGAAAGGG GACCCTGGCC TACCAGGTAT TCCTGGAGTT 
GCAGGGCCAA AAGGTTACCA GGGTTTACCT GGAGACCCAG GGCAACCTGG ACTGAGTGGA 
CAACCTGGAT TGCCAGGACC AGCAGGTCCC AAGGGTAACC CTGGTCTCCC TGGAATGCCA 
GGACTTACAG GACCTCCTGG ACTGAAAGGA AACATGGGTG ATATGGGTTT TCCAGGCCCA 
CAGGGTCCAA AAGGGTCTTT CGGACCTCCT GGAGTTCCTG GACAACCTGG CTCCCCAGGA 
TTACCTGGAC AGAAAGGAGA AAAAGGTGAT CCTGGTATTT CAGGCATTGG TCTTCCAGGT 
CTTCCTGGCC CAAAGGGTGA ACCTGGTCTG CCTGGATACC CAGGGAACCC TGGTACCAAA 
GGTTCTATGG GAGAAACTGG TTTGCCTGGA TTACCGGGGA CCCCTGGAGC AAAAGGACAA 
CCAGGCCTTC CTGGATTCCC TGGAACCCCA GGCCTTCCTG GACCAAAAGG TATTAGTGGT 
CCTCCTGGGA ACCCTGGCCT TCCAGGAGAA CCTGGTCCTG TAGGTGGTGG AGGTCGTCCT 
GGGCCACCAG GGCCTCCAGG TGAAAAAGGC AAACCAGGTC AAGATGGTAT TCCTGGACCA 
GCTGGACAGA AGGGTGAACC AGGTCAACCA GGCTTTGGAA TCCCAGGACC CCCTGGACTT 
CCAGGACTTT CTGGTCAAAA GGGTGATGAA GGATTACCTG GCATTCCAGG AAACCCTGGC 
CTTCCAGGTC CAAAGGGTGA ACCAGGCTTT CATGGTTTCC CTGGTCTGCA GGGTCCCCCA 
GGCCCTCCTG GTTCTCCAGG TCCAGCTCTG GAAGGCCCTA AAGGCAGCCC TGGACCCCAA 
GGTCCTCCTG GGAGACCAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA 
CCTGGAAGTG GGGGTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT 
TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG 
AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC 
CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGTCAGA GTATTGTAAT CAAAGGAGAT 
GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA 
CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT 
CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA 
ACCTCTTCTA TCGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA 
CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT 
TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT 
GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG 
ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG 
GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG 
CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG 
GCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102399306 ( XM_006072344.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006072406.1 Bubalus bubalis collagen alpha-5(IV) chain-like (LOC102399306), transcript variant X4, mRNA.

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CloneID	OBu26604
Clone ID Related Accession (Same CDS sequence)	XM_006072344.1
Accession Version	XM_006072344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-5(IV) chain-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785484.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTCCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CTATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGC CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC CAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAACCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTTTTCAAGG TCCACCAGGT CCAGAAGGTC CCCGGGGTCT ACCTGGAAGT 
GGGGGTATTA AAGGAGAAAG AGGTAACCCA GGCCAACCTG GGCAACCTGG TTTGCCTGGT 
TTGAAAGGTG ATCAAGGTCC TCCAGGACTC CAGGGTAACC CTGGCCGGCC AGGTCTCAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TTCCAGGCAT GAAAGGACCC 
AGTGGAGTAC CTGGTTCAAC TGGCCCTGAG GGGGATCCAG GACTTATTGG CCCACCAGGT 
CCCCCTGGAT TACCTGGTCC TTCAGGTCAG AGTATTGTAA TCAAAGGAGA TGCTGGCCCA 
CCAGGGATTC CAGGCCAGCC TGGATTAAAA GGTTTACAAG GACTCCCAGG ACCTCAGGGT 
TTACCAGGTC CAATTGGCCC TCCAGGAGAT CCTGGACGCA ATGGACTCCC TGGCTTTGAT 
GGTGCAGGAG GACGCAAAGG AGACCCAGGT TTGCCAGGCC AGCCAGGTAC TCGTGGCTTG 
GATGGTCCCC CCGGGCCAGA TGGATTGCAG GGTCCTCCAG GTCCCCCTGG AACCTCTTCT 
ATCGCCCATG GGTTTCTCAT CACACGTCAC AGCCAGACAA CAGATGCTCC ACAATGCCCA 
CAGGGAACCA TCCAGGTGTA TGAAGGCTTT TCTCTCCTGT ATGTACAAGG AAATAAAAGA 
GCTCATGGTC AAGACTTGGG AACAGCCGGC AGCTGCCTTC GTCGTTTCAG TACCATGCCT 
TTCATGTTCT GCAACATCAA TAATGTTTGC AACTTTGCTT CAAGAAATGA TTATTCTTAC 
TGGCTCTCCA CCCCAGAGCC CATGCCAATG AGCATGGAAC CCCTGAAGGG GCAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTATGT GAAGCTCCGG CCGTGGTGAT TGCAGTCCAC 
AGTCAGACCA TCCAGATTCC CCGTTGTCCT CAGGGATGGG ATTCTCTCTG GATTGGTTAT 
TCCTTCATGA TGCACACAAG TGCAGGGGCA GAGGGCTCAG GCCAAGCCCT GGCCTCCCCT 
GGTTCCTGCT TAGAAGAGTT TCGTTCAGCT CCCTTCATTG AGTGTCATGG GCGGGGGACC 
TGCAACTATT ACGCCAACTC CTACAGCTTT TGGCTGGCGA CTGTAGATGT GGCAGACATG 
TTCAGCAAAC CTCAATCAGA AACGCTGAAA GCAGGAGACT TGAGGACACG TATTAGCCGA 
TGTCAAGTGT GCATGAAGAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006072406.1
CDS	321..5387
Translation	MKLRGVSLAAGLSLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGPAGPPGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGVKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGLPGPPGLVIPRPGTGVTVGEKGNIGLPGLPGDKGDRGFPGIQGPPGLPGPPGPAVIGPPGPPGFPGERGQKGDEGPPGISIPGSPGLDGQPGAPGPPGPPGPPGPHIPPSDKICEAGPPGPPGSPGDRGLQGEQGMKGDKGDTCFNCIGTGVSGPPGQPGLPGLPGPPGSLGFPGQKGEKGHAGATGPKGLTGIPGAPGAPGFPGPKGEPGDILTFPGMKGDKGDLGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGMAFKGERGPPGNPGLPGLPGNRGPMGPVGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGHPGRDGEVGLPGEPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGSPGRIGLEGPSGPPGFPGPKGEPGFGLPGPPGPPGLPGFKGILGPKGDRGFPGPQGPPGQAGLDGLPGPKGDIGPNGQPGPIGPPGLPGTGFQGPPGPPGIPGPIGQPGLHGLPGEKGDPGPPGFDVPGPPGERGSPGIPGAPGPMGPPGSPGLPGKAGASGFPGAKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGLPGPMDPDLLGSKGEKGDPGLPGIPGVAGPKGYQGLPGDPGQPGLSGQPGLPGPAGPKGNPGLPGMPGLTGPPGLKGNMGDMGFPGPQGPKGSFGPPGVPGQPGSPGLPGQKGEKGDPGISGIGLPGLPGPKGEPGLPGYPGNPGTKGSMGETGLPGLPGTPGAKGQPGLPGFPGTPGLPGPKGISGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDEGLPGIPGNPGLPGPKGEPGFHGFPGLQGPPGPPGSPGPALEGPKGSPGPQGPPGRPGFQGPPGPEGPRGLPGSGGIKGERGNPGQPGQPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSTGPEGDPGLIGPPGPPGLPGPSGQSIVIKGDAGPPGIPGQPGLKGLQGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTIQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVADMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_006072344.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen alpha-5(IV) chain-like (LOC102399306), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006072344.1

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTCCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CTATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGC CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC CAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAACCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTTTTCAAGG TCCACCAGGT CCAGAAGGTC CCCGGGGTCT ACCTGGAAGT 
GGGGGTATTA AAGGAGAAAG AGGTAACCCA GGCCAACCTG GGCAACCTGG TTTGCCTGGT 
TTGAAAGGTG ATCAAGGTCC TCCAGGACTC CAGGGTAACC CTGGCCGGCC AGGTCTCAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TTCCAGGCAT GAAAGGACCC 
AGTGGAGTAC CTGGTTCAAC TGGCCCTGAG GGGGATCCAG GACTTATTGG CCCACCAGGT 
CCCCCTGGAT TACCTGGTCC TTCAGGTCAG AGTATTGTAA TCAAAGGAGA TGCTGGCCCA 
CCAGGGATTC CAGGCCAGCC TGGATTAAAA GGTTTACAAG GACTCCCAGG ACCTCAGGGT 
TTACCAGGTC CAATTGGCCC TCCAGGAGAT CCTGGACGCA ATGGACTCCC TGGCTTTGAT 
GGTGCAGGAG GACGCAAAGG AGACCCAGGT TTGCCAGGCC AGCCAGGTAC TCGTGGCTTG 
GATGGTCCCC CCGGGCCAGA TGGATTGCAG GGTCCTCCAG GTCCCCCTGG AACCTCTTCT 
ATCGCCCATG GGTTTCTCAT CACACGTCAC AGCCAGACAA CAGATGCTCC ACAATGCCCA 
CAGGGAACCA TCCAGGTGTA TGAAGGCTTT TCTCTCCTGT ATGTACAAGG AAATAAAAGA 
GCTCATGGTC AAGACTTGGG AACAGCCGGC AGCTGCCTTC GTCGTTTCAG TACCATGCCT 
TTCATGTTCT GCAACATCAA TAATGTTTGC AACTTTGCTT CAAGAAATGA TTATTCTTAC 
TGGCTCTCCA CCCCAGAGCC CATGCCAATG AGCATGGAAC CCCTGAAGGG GCAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTATGT GAAGCTCCGG CCGTGGTGAT TGCAGTCCAC 
AGTCAGACCA TCCAGATTCC CCGTTGTCCT CAGGGATGGG ATTCTCTCTG GATTGGTTAT 
TCCTTCATGA TGCACACAAG TGCAGGGGCA GAGGGCTCAG GCCAAGCCCT GGCCTCCCCT 
GGTTCCTGCT TAGAAGAGTT TCGTTCAGCT CCCTTCATTG AGTGTCATGG GCGGGGGACC 
TGCAACTATT ACGCCAACTC CTACAGCTTT TGGCTGGCGA CTGTAGATGT GGCAGACATG 
TTCAGCAAAC CTCAATCAGA AACGCTGAAA GCAGGAGACT TGAGGACACG TATTAGCCGA 
TGTCAAGTGT GCATGAAGAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102399306 ( XM_006072345.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006072407.1 Bubalus bubalis collagen type IV alpha 5 chain (COL4A5), transcript variant X5, mRNA.

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CloneID	OBu26605
Clone ID Related Accession (Same CDS sequence)	XM_006072345.2 , XM_006072345.1
Accession Version	XM_006072345.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-5(IV) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006072345.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG TCTACCAGGC 
TTGCATGGAC TACCAGGAGA GAAGGGGGAT CCAGGGCCTC CTGGATTTGA TGTTCCAGGA 
CCTCCTGGTG AGAGAGGCAG CCCAGGGATC CCTGGAGCAC CAGGTCCTAT GGGACCCCCA 
GGATCACCAG GGCTCCCAGG AAAAGCAGGT GCCTCTGGCT TTCCAGGTGC CAAAGGTGAA 
ATGGGTATGA TGGGACCTCC AGGCCCACCA GGACCTTTGG GAATTCCTGG CAGGAGTGGT 
GTTCCTGGTC TCAAAGGTGA TGATGGTTTG CAGGGTCAGC CAGGACTTCC TGGCCCTGCA 
GGAGAGAAAG GTAGTAAAGG AGAGCCTGGC CTTCCAGGCC TTCCTGGGCC TATGGATCCA 
GATCTGCTGG GCTCAAAAGG AGAGAAAGGG GACCCTGGCC TACCAGGTAT TCCTGGAGTT 
GCAGGGCCAA AAGGTTACCA GGGTTTACCT GGAGACCCAG GGCAACCTGG ACTGAGTGGA 
CAACCTGGAT TGCCAGGACC AGCAGGTCCC AAGGGTAACC CTGGTCTCCC TGGAATGCCA 
GGACTTACAG GACCTCCTGG ACTGAAAGGA AACATGGGTG ATATGGGTTT TCCAGGCCCA 
CAGGGTCCAA AAGGGTCTTT CGGACCTCCT GGAGTTCCTG GACAACCTGG CTCCCCAGGA 
TTACCTGGAC AGAAAGGAGA AAAAGGTGAT CCTGGTATTT CAGGCATTGG TCTTCCAGGT 
CTTCCTGGCC CAAAGGGTGA ACCTGGTCTG CCTGGATACC CAGGGAACCC TGGTACCAAA 
GGTTCTATGG GAGAAACTGG TTTGCCTGGA TTACCGGGGA CCCCTGGAGC AAAAGGACAA 
CCAGGCCTTC CTGGATTCCC TGGAACCCCA GGCCTTCCTG GACCAAAAGG TATTAGTGGT 
CCTCCTGGGA ACCCTGGCCT TCCAGGAGAA CCTGGTCCTG TAGGTGGTGG AGGTCGTCCT 
GGGCCACCAG GGCCTCCAGG TGAAAAAGGC AAACCAGGTC AAGATGGTAT TCCTGGACCA 
GCTGGACAGA AGGGTGAACC AGGTCAACCA GGCTTTGGAA TCCCAGGACC CCCTGGACTT 
CCAGGACTTT CTGGTCAAAA GGGTGATGAA GGATTACCTG GCATTCCAGG AAACCCTGGC 
CTTCCAGGTC CAAAGGGTGA ACCAGGCTTT CATGGTTTCC CTGGTCTGCA GGGTCCCCCA 
GGCCCTCCTG GTTCTCCAGG TCCAGCTCTG GAAGGCCCTA AAGGCAGCCC TGGACCCCAA 
GGTCCTCCTG GGAGACCAGG TCCACCAGGT CCAGAAGGTC CCCGGGGTCT ACCTGGAAGT 
GGGGGTATTA AAGGAGAAAG AGGTAACCCA GGCCAACCTG GGCAACCTGG TTTGCCTGGT 
TTGAAAGGTG ATCAAGGTCC TCCAGGACTC CAGGGTAACC CTGGCCGGCC AGGTCTCAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TTCCAGGCAT GAAAGGACCC 
AGTGGAGTAC CTGGTTCAAC TGGCCCTGAG GGGGATCCAG GACTTATTGG CCCACCAGGT 
CCCCCTGGAT TACCTGGTCC TTCAGGTCAG AGTATTGTAA TCAAAGGAGA TGCTGGCCCA 
CCAGGGATTC CAGGCCAGCC TGGATTAAAA GGTTTACAAG GACTCCCAGG ACCTCAGGGT 
TTACCAGGTC CAATTGGCCC TCCAGGAGAT CCTGGACGCA ATGGACTCCC TGGCTTTGAT 
GGTGCAGGAG GACGCAAAGG AGACCCAGGT TTGCCAGGCC AGCCAGGTAC TCGTGGCTTG 
GATGGTCCCC CCGGGCCAGA TGGATTGCAG GGTCCTCCAG GTCCCCCTGG AACCTCTTCT 
ATCGCCCATG GGTTTCTCAT CACACGTCAC AGCCAGACAA CAGATGCTCC ACAATGCCCA 
CAGGGAACCA TCCAGGTGTA TGAAGGCTTT TCTCTCCTGT ATGTACAAGG AAATAAAAGA 
GCTCATGGTC AAGACTTGGG AACAGCCGGC AGCTGCCTTC GTCGTTTCAG TACCATGCCT 
TTCATGTTCT GCAACATCAA TAATGTTTGC AACTTTGCTT CAAGAAATGA TTATTCTTAC 
TGGCTCTCCA CCCCAGAGCC CATGCCAATG AGCATGGAAC CCCTGAAGGG GCAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTATGT GAAGCTCCGG CCGTGGTGAT TGCAGTCCAC 
AGTCAGACCA TCCAGATTCC CCGTTGTCCT CAGGGATGGG ATTCTCTCTG GATTGGTTAT 
TCCTTCATGA TGCACACAAG TGCAGGGGCA GAGGGCTCAG GCCAAGCCCT GGCCTCCCCT 
GGTTCCTGCT TAGAAGAGTT TCGTTCAGCT CCCTTCATTG AGTGTCATGG GCGGGGGACC 
TGCAACTATT ACGCCAACTC CTACAGCTTT TGGCTGGCGA CTGTAGATGT GGCAGACATG 
TTCAGCAAAC CTCAATCAGA AACGCTGAAA GCAGGAGACT TGAGGACACG TATTAGCCGA 
TGTCAAGTGT GCATGAAGAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006072407.1
CDS	328..5394
Translation	MKLRGVSLAAGLSLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGPAGPPGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGVKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGLPGPPGLVIPRPGTGVTVGEKGNIGLPGLPGDKGDRGFPGIQGPPGLPGPPGPAVIGPPGPPGFPGERGQKGDEGPPGISIPGSPGLDGQPGAPGPPGPPGPPGPHIPPSDKICEAGPPGPPGSPGDRGLQGEQGMKGDKGDTCFNCIGTGVSGPPGQPGLPGLPGPPGSLGFPGQKGEKGHAGATGPKGLTGIPGAPGAPGFPGPKGEPGDILTFPGMKGDKGDLGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGMAFKGERGPPGNPGLPGLPGNRGPMGPVGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGHPGRDGEVGLPGEPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGSPGRIGLEGPSGPPGFPGPKGEPGFGLPGPPGPPGLPGFKGILGPKGDRGFPGPQGPPGQAGLDGLPGPKGDIGPNGQPGPIGPPGLPGTGFQGPPGPPGIPGPIGQPGLPGLHGLPGEKGDPGPPGFDVPGPPGERGSPGIPGAPGPMGPPGSPGLPGKAGASGFPGAKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGLPGPMDPDLLGSKGEKGDPGLPGIPGVAGPKGYQGLPGDPGQPGLSGQPGLPGPAGPKGNPGLPGMPGLTGPPGLKGNMGDMGFPGPQGPKGSFGPPGVPGQPGSPGLPGQKGEKGDPGISGIGLPGLPGPKGEPGLPGYPGNPGTKGSMGETGLPGLPGTPGAKGQPGLPGFPGTPGLPGPKGISGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDEGLPGIPGNPGLPGPKGEPGFHGFPGLQGPPGPPGSPGPALEGPKGSPGPQGPPGRPGPPGPEGPRGLPGSGGIKGERGNPGQPGQPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSTGPEGDPGLIGPPGPPGLPGPSGQSIVIKGDAGPPGIPGQPGLKGLQGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTIQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVADMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_006072345.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen type IV alpha 5 chain (COL4A5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006072345.2

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG TCTACCAGGC 
TTGCATGGAC TACCAGGAGA GAAGGGGGAT CCAGGGCCTC CTGGATTTGA TGTTCCAGGA 
CCTCCTGGTG AGAGAGGCAG CCCAGGGATC CCTGGAGCAC CAGGTCCTAT GGGACCCCCA 
GGATCACCAG GGCTCCCAGG AAAAGCAGGT GCCTCTGGCT TTCCAGGTGC CAAAGGTGAA 
ATGGGTATGA TGGGACCTCC AGGCCCACCA GGACCTTTGG GAATTCCTGG CAGGAGTGGT 
GTTCCTGGTC TCAAAGGTGA TGATGGTTTG CAGGGTCAGC CAGGACTTCC TGGCCCTGCA 
GGAGAGAAAG GTAGTAAAGG AGAGCCTGGC CTTCCAGGCC TTCCTGGGCC TATGGATCCA 
GATCTGCTGG GCTCAAAAGG AGAGAAAGGG GACCCTGGCC TACCAGGTAT TCCTGGAGTT 
GCAGGGCCAA AAGGTTACCA GGGTTTACCT GGAGACCCAG GGCAACCTGG ACTGAGTGGA 
CAACCTGGAT TGCCAGGACC AGCAGGTCCC AAGGGTAACC CTGGTCTCCC TGGAATGCCA 
GGACTTACAG GACCTCCTGG ACTGAAAGGA AACATGGGTG ATATGGGTTT TCCAGGCCCA 
CAGGGTCCAA AAGGGTCTTT CGGACCTCCT GGAGTTCCTG GACAACCTGG CTCCCCAGGA 
TTACCTGGAC AGAAAGGAGA AAAAGGTGAT CCTGGTATTT CAGGCATTGG TCTTCCAGGT 
CTTCCTGGCC CAAAGGGTGA ACCTGGTCTG CCTGGATACC CAGGGAACCC TGGTACCAAA 
GGTTCTATGG GAGAAACTGG TTTGCCTGGA TTACCGGGGA CCCCTGGAGC AAAAGGACAA 
CCAGGCCTTC CTGGATTCCC TGGAACCCCA GGCCTTCCTG GACCAAAAGG TATTAGTGGT 
CCTCCTGGGA ACCCTGGCCT TCCAGGAGAA CCTGGTCCTG TAGGTGGTGG AGGTCGTCCT 
GGGCCACCAG GGCCTCCAGG TGAAAAAGGC AAACCAGGTC AAGATGGTAT TCCTGGACCA 
GCTGGACAGA AGGGTGAACC AGGTCAACCA GGCTTTGGAA TCCCAGGACC CCCTGGACTT 
CCAGGACTTT CTGGTCAAAA GGGTGATGAA GGATTACCTG GCATTCCAGG AAACCCTGGC 
CTTCCAGGTC CAAAGGGTGA ACCAGGCTTT CATGGTTTCC CTGGTCTGCA GGGTCCCCCA 
GGCCCTCCTG GTTCTCCAGG TCCAGCTCTG GAAGGCCCTA AAGGCAGCCC TGGACCCCAA 
GGTCCTCCTG GGAGACCAGG TCCACCAGGT CCAGAAGGTC CCCGGGGTCT ACCTGGAAGT 
GGGGGTATTA AAGGAGAAAG AGGTAACCCA GGCCAACCTG GGCAACCTGG TTTGCCTGGT 
TTGAAAGGTG ATCAAGGTCC TCCAGGACTC CAGGGTAACC CTGGCCGGCC AGGTCTCAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TTCCAGGCAT GAAAGGACCC 
AGTGGAGTAC CTGGTTCAAC TGGCCCTGAG GGGGATCCAG GACTTATTGG CCCACCAGGT 
CCCCCTGGAT TACCTGGTCC TTCAGGTCAG AGTATTGTAA TCAAAGGAGA TGCTGGCCCA 
CCAGGGATTC CAGGCCAGCC TGGATTAAAA GGTTTACAAG GACTCCCAGG ACCTCAGGGT 
TTACCAGGTC CAATTGGCCC TCCAGGAGAT CCTGGACGCA ATGGACTCCC TGGCTTTGAT 
GGTGCAGGAG GACGCAAAGG AGACCCAGGT TTGCCAGGCC AGCCAGGTAC TCGTGGCTTG 
GATGGTCCCC CCGGGCCAGA TGGATTGCAG GGTCCTCCAG GTCCCCCTGG AACCTCTTCT 
ATCGCCCATG GGTTTCTCAT CACACGTCAC AGCCAGACAA CAGATGCTCC ACAATGCCCA 
CAGGGAACCA TCCAGGTGTA TGAAGGCTTT TCTCTCCTGT ATGTACAAGG AAATAAAAGA 
GCTCATGGTC AAGACTTGGG AACAGCCGGC AGCTGCCTTC GTCGTTTCAG TACCATGCCT 
TTCATGTTCT GCAACATCAA TAATGTTTGC AACTTTGCTT CAAGAAATGA TTATTCTTAC 
TGGCTCTCCA CCCCAGAGCC CATGCCAATG AGCATGGAAC CCCTGAAGGG GCAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTATGT GAAGCTCCGG CCGTGGTGAT TGCAGTCCAC 
AGTCAGACCA TCCAGATTCC CCGTTGTCCT CAGGGATGGG ATTCTCTCTG GATTGGTTAT 
TCCTTCATGA TGCACACAAG TGCAGGGGCA GAGGGCTCAG GCCAAGCCCT GGCCTCCCCT 
GGTTCCTGCT TAGAAGAGTT TCGTTCAGCT CCCTTCATTG AGTGTCATGG GCGGGGGACC 
TGCAACTATT ACGCCAACTC CTACAGCTTT TGGCTGGCGA CTGTAGATGT GGCAGACATG 
TTCAGCAAAC CTCAATCAGA AACGCTGAAA GCAGGAGACT TGAGGACACG TATTAGCCGA 
TGTCAAGTGT GCATGAAGAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102399306 ( XM_006072345.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006072407.1 Bubalus bubalis collagen alpha-5(IV) chain-like (LOC102399306), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu26605
Clone ID Related Accession (Same CDS sequence)	XM_006072345.2 , XM_006072345.1
Accession Version	XM_006072345.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-5(IV) chain-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785484.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG TCTACCAGGC 
TTGCATGGAC TACCAGGAGA GAAGGGGGAT CCAGGGCCTC CTGGATTTGA TGTTCCAGGA 
CCTCCTGGTG AGAGAGGCAG CCCAGGGATC CCTGGAGCAC CAGGTCCTAT GGGACCCCCA 
GGATCACCAG GGCTCCCAGG AAAAGCAGGT GCCTCTGGCT TTCCAGGTGC CAAAGGTGAA 
ATGGGTATGA TGGGACCTCC AGGCCCACCA GGACCTTTGG GAATTCCTGG CAGGAGTGGT 
GTTCCTGGTC TCAAAGGTGA TGATGGTTTG CAGGGTCAGC CAGGACTTCC TGGCCCTGCA 
GGAGAGAAAG GTAGTAAAGG AGAGCCTGGC CTTCCAGGCC TTCCTGGGCC TATGGATCCA 
GATCTGCTGG GCTCAAAAGG AGAGAAAGGG GACCCTGGCC TACCAGGTAT TCCTGGAGTT 
GCAGGGCCAA AAGGTTACCA GGGTTTACCT GGAGACCCAG GGCAACCTGG ACTGAGTGGA 
CAACCTGGAT TGCCAGGACC AGCAGGTCCC AAGGGTAACC CTGGTCTCCC TGGAATGCCA 
GGACTTACAG GACCTCCTGG ACTGAAAGGA AACATGGGTG ATATGGGTTT TCCAGGCCCA 
CAGGGTCCAA AAGGGTCTTT CGGACCTCCT GGAGTTCCTG GACAACCTGG CTCCCCAGGA 
TTACCTGGAC AGAAAGGAGA AAAAGGTGAT CCTGGTATTT CAGGCATTGG TCTTCCAGGT 
CTTCCTGGCC CAAAGGGTGA ACCTGGTCTG CCTGGATACC CAGGGAACCC TGGTACCAAA 
GGTTCTATGG GAGAAACTGG TTTGCCTGGA TTACCGGGGA CCCCTGGAGC AAAAGGACAA 
CCAGGCCTTC CTGGATTCCC TGGAACCCCA GGCCTTCCTG GACCAAAAGG TATTAGTGGT 
CCTCCTGGGA ACCCTGGCCT TCCAGGAGAA CCTGGTCCTG TAGGTGGTGG AGGTCGTCCT 
GGGCCACCAG GGCCTCCAGG TGAAAAAGGC AAACCAGGTC AAGATGGTAT TCCTGGACCA 
GCTGGACAGA AGGGTGAACC AGGTCAACCA GGCTTTGGAA TCCCAGGACC CCCTGGACTT 
CCAGGACTTT CTGGTCAAAA GGGTGATGAA GGATTACCTG GCATTCCAGG AAACCCTGGC 
CTTCCAGGTC CAAAGGGTGA ACCAGGCTTT CATGGTTTCC CTGGTCTGCA GGGTCCCCCA 
GGCCCTCCTG GTTCTCCAGG TCCAGCTCTG GAAGGCCCTA AAGGCAGCCC TGGACCCCAA 
GGTCCTCCTG GGAGACCAGG TCCACCAGGT CCAGAAGGTC CCCGGGGTCT ACCTGGAAGT 
GGGGGTATTA AAGGAGAAAG AGGTAACCCA GGCCAACCTG GGCAACCTGG TTTGCCTGGT 
TTGAAAGGTG ATCAAGGTCC TCCAGGACTC CAGGGTAACC CTGGCCGGCC AGGTCTCAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TTCCAGGCAT GAAAGGACCC 
AGTGGAGTAC CTGGTTCAAC TGGCCCTGAG GGGGATCCAG GACTTATTGG CCCACCAGGT 
CCCCCTGGAT TACCTGGTCC TTCAGGTCAG AGTATTGTAA TCAAAGGAGA TGCTGGCCCA 
CCAGGGATTC CAGGCCAGCC TGGATTAAAA GGTTTACAAG GACTCCCAGG ACCTCAGGGT 
TTACCAGGTC CAATTGGCCC TCCAGGAGAT CCTGGACGCA ATGGACTCCC TGGCTTTGAT 
GGTGCAGGAG GACGCAAAGG AGACCCAGGT TTGCCAGGCC AGCCAGGTAC TCGTGGCTTG 
GATGGTCCCC CCGGGCCAGA TGGATTGCAG GGTCCTCCAG GTCCCCCTGG AACCTCTTCT 
ATCGCCCATG GGTTTCTCAT CACACGTCAC AGCCAGACAA CAGATGCTCC ACAATGCCCA 
CAGGGAACCA TCCAGGTGTA TGAAGGCTTT TCTCTCCTGT ATGTACAAGG AAATAAAAGA 
GCTCATGGTC AAGACTTGGG AACAGCCGGC AGCTGCCTTC GTCGTTTCAG TACCATGCCT 
TTCATGTTCT GCAACATCAA TAATGTTTGC AACTTTGCTT CAAGAAATGA TTATTCTTAC 
TGGCTCTCCA CCCCAGAGCC CATGCCAATG AGCATGGAAC CCCTGAAGGG GCAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTATGT GAAGCTCCGG CCGTGGTGAT TGCAGTCCAC 
AGTCAGACCA TCCAGATTCC CCGTTGTCCT CAGGGATGGG ATTCTCTCTG GATTGGTTAT 
TCCTTCATGA TGCACACAAG TGCAGGGGCA GAGGGCTCAG GCCAAGCCCT GGCCTCCCCT 
GGTTCCTGCT TAGAAGAGTT TCGTTCAGCT CCCTTCATTG AGTGTCATGG GCGGGGGACC 
TGCAACTATT ACGCCAACTC CTACAGCTTT TGGCTGGCGA CTGTAGATGT GGCAGACATG 
TTCAGCAAAC CTCAATCAGA AACGCTGAAA GCAGGAGACT TGAGGACACG TATTAGCCGA 
TGTCAAGTGT GCATGAAGAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006072407.1
CDS	321..5387
Translation	MKLRGVSLAAGLSLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGPAGPPGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGVKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGLPGPPGLVIPRPGTGVTVGEKGNIGLPGLPGDKGDRGFPGIQGPPGLPGPPGPAVIGPPGPPGFPGERGQKGDEGPPGISIPGSPGLDGQPGAPGPPGPPGPPGPHIPPSDKICEAGPPGPPGSPGDRGLQGEQGMKGDKGDTCFNCIGTGVSGPPGQPGLPGLPGPPGSLGFPGQKGEKGHAGATGPKGLTGIPGAPGAPGFPGPKGEPGDILTFPGMKGDKGDLGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGMAFKGERGPPGNPGLPGLPGNRGPMGPVGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGHPGRDGEVGLPGEPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGSPGRIGLEGPSGPPGFPGPKGEPGFGLPGPPGPPGLPGFKGILGPKGDRGFPGPQGPPGQAGLDGLPGPKGDIGPNGQPGPIGPPGLPGTGFQGPPGPPGIPGPIGQPGLPGLHGLPGEKGDPGPPGFDVPGPPGERGSPGIPGAPGPMGPPGSPGLPGKAGASGFPGAKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGLPGPMDPDLLGSKGEKGDPGLPGIPGVAGPKGYQGLPGDPGQPGLSGQPGLPGPAGPKGNPGLPGMPGLTGPPGLKGNMGDMGFPGPQGPKGSFGPPGVPGQPGSPGLPGQKGEKGDPGISGIGLPGLPGPKGEPGLPGYPGNPGTKGSMGETGLPGLPGTPGAKGQPGLPGFPGTPGLPGPKGISGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDEGLPGIPGNPGLPGPKGEPGFHGFPGLQGPPGPPGSPGPALEGPKGSPGPQGPPGRPGPPGPEGPRGLPGSGGIKGERGNPGQPGQPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSTGPEGDPGLIGPPGPPGLPGPSGQSIVIKGDAGPPGIPGQPGLKGLQGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTIQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVADMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_006072345.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen alpha-5(IV) chain-like (LOC102399306), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006072345.1

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG TCTACCAGGC 
TTGCATGGAC TACCAGGAGA GAAGGGGGAT CCAGGGCCTC CTGGATTTGA TGTTCCAGGA 
CCTCCTGGTG AGAGAGGCAG CCCAGGGATC CCTGGAGCAC CAGGTCCTAT GGGACCCCCA 
GGATCACCAG GGCTCCCAGG AAAAGCAGGT GCCTCTGGCT TTCCAGGTGC CAAAGGTGAA 
ATGGGTATGA TGGGACCTCC AGGCCCACCA GGACCTTTGG GAATTCCTGG CAGGAGTGGT 
GTTCCTGGTC TCAAAGGTGA TGATGGTTTG CAGGGTCAGC CAGGACTTCC TGGCCCTGCA 
GGAGAGAAAG GTAGTAAAGG AGAGCCTGGC CTTCCAGGCC TTCCTGGGCC TATGGATCCA 
GATCTGCTGG GCTCAAAAGG AGAGAAAGGG GACCCTGGCC TACCAGGTAT TCCTGGAGTT 
GCAGGGCCAA AAGGTTACCA GGGTTTACCT GGAGACCCAG GGCAACCTGG ACTGAGTGGA 
CAACCTGGAT TGCCAGGACC AGCAGGTCCC AAGGGTAACC CTGGTCTCCC TGGAATGCCA 
GGACTTACAG GACCTCCTGG ACTGAAAGGA AACATGGGTG ATATGGGTTT TCCAGGCCCA 
CAGGGTCCAA AAGGGTCTTT CGGACCTCCT GGAGTTCCTG GACAACCTGG CTCCCCAGGA 
TTACCTGGAC AGAAAGGAGA AAAAGGTGAT CCTGGTATTT CAGGCATTGG TCTTCCAGGT 
CTTCCTGGCC CAAAGGGTGA ACCTGGTCTG CCTGGATACC CAGGGAACCC TGGTACCAAA 
GGTTCTATGG GAGAAACTGG TTTGCCTGGA TTACCGGGGA CCCCTGGAGC AAAAGGACAA 
CCAGGCCTTC CTGGATTCCC TGGAACCCCA GGCCTTCCTG GACCAAAAGG TATTAGTGGT 
CCTCCTGGGA ACCCTGGCCT TCCAGGAGAA CCTGGTCCTG TAGGTGGTGG AGGTCGTCCT 
GGGCCACCAG GGCCTCCAGG TGAAAAAGGC AAACCAGGTC AAGATGGTAT TCCTGGACCA 
GCTGGACAGA AGGGTGAACC AGGTCAACCA GGCTTTGGAA TCCCAGGACC CCCTGGACTT 
CCAGGACTTT CTGGTCAAAA GGGTGATGAA GGATTACCTG GCATTCCAGG AAACCCTGGC 
CTTCCAGGTC CAAAGGGTGA ACCAGGCTTT CATGGTTTCC CTGGTCTGCA GGGTCCCCCA 
GGCCCTCCTG GTTCTCCAGG TCCAGCTCTG GAAGGCCCTA AAGGCAGCCC TGGACCCCAA 
GGTCCTCCTG GGAGACCAGG TCCACCAGGT CCAGAAGGTC CCCGGGGTCT ACCTGGAAGT 
GGGGGTATTA AAGGAGAAAG AGGTAACCCA GGCCAACCTG GGCAACCTGG TTTGCCTGGT 
TTGAAAGGTG ATCAAGGTCC TCCAGGACTC CAGGGTAACC CTGGCCGGCC AGGTCTCAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TTCCAGGCAT GAAAGGACCC 
AGTGGAGTAC CTGGTTCAAC TGGCCCTGAG GGGGATCCAG GACTTATTGG CCCACCAGGT 
CCCCCTGGAT TACCTGGTCC TTCAGGTCAG AGTATTGTAA TCAAAGGAGA TGCTGGCCCA 
CCAGGGATTC CAGGCCAGCC TGGATTAAAA GGTTTACAAG GACTCCCAGG ACCTCAGGGT 
TTACCAGGTC CAATTGGCCC TCCAGGAGAT CCTGGACGCA ATGGACTCCC TGGCTTTGAT 
GGTGCAGGAG GACGCAAAGG AGACCCAGGT TTGCCAGGCC AGCCAGGTAC TCGTGGCTTG 
GATGGTCCCC CCGGGCCAGA TGGATTGCAG GGTCCTCCAG GTCCCCCTGG AACCTCTTCT 
ATCGCCCATG GGTTTCTCAT CACACGTCAC AGCCAGACAA CAGATGCTCC ACAATGCCCA 
CAGGGAACCA TCCAGGTGTA TGAAGGCTTT TCTCTCCTGT ATGTACAAGG AAATAAAAGA 
GCTCATGGTC AAGACTTGGG AACAGCCGGC AGCTGCCTTC GTCGTTTCAG TACCATGCCT 
TTCATGTTCT GCAACATCAA TAATGTTTGC AACTTTGCTT CAAGAAATGA TTATTCTTAC 
TGGCTCTCCA CCCCAGAGCC CATGCCAATG AGCATGGAAC CCCTGAAGGG GCAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTATGT GAAGCTCCGG CCGTGGTGAT TGCAGTCCAC 
AGTCAGACCA TCCAGATTCC CCGTTGTCCT CAGGGATGGG ATTCTCTCTG GATTGGTTAT 
TCCTTCATGA TGCACACAAG TGCAGGGGCA GAGGGCTCAG GCCAAGCCCT GGCCTCCCCT 
GGTTCCTGCT TAGAAGAGTT TCGTTCAGCT CCCTTCATTG AGTGTCATGG GCGGGGGACC 
TGCAACTATT ACGCCAACTC CTACAGCTTT TGGCTGGCGA CTGTAGATGT GGCAGACATG 
TTCAGCAAAC CTCAATCAGA AACGCTGAAA GCAGGAGACT TGAGGACACG TATTAGCCGA 
TGTCAAGTGT GCATGAAGAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102399306 ( XM_006072346.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006072408.1 Bubalus bubalis collagen alpha-5(IV) chain-like (LOC102399306), transcript variant X6, mRNA.

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CloneID	OBu26606
Clone ID Related Accession (Same CDS sequence)	XM_006072346.1 , XM_006072346.2
Accession Version	XM_006072346.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-5(IV) chain-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785484.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTCCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CTATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGC CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC CAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAACCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCACCAGG TCCAGAAGGT CCCCGGGGTC TACCTGGAAG TGGGGGTATT 
AAAGGAGAAA GAGGTAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG TTTGAAAGGT 
GATCAAGGTC CTCCAGGACT CCAGGGTAAC CCTGGCCGGC CAGGTCTCAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGCA TGAAAGGACC CAGTGGAGTA 
CCTGGTTCAA CTGGCCCTGA GGGGGATCCA GGACTTATTG GCCCACCAGG TCCCCCTGGA 
TTACCTGGTC CTTCAGGTCA GAGTATTGTA ATCAAAGGAG ATGCTGGCCC ACCAGGGATT 
CCAGGCCAGC CTGGATTAAA AGGTTTACAA GGACTCCCAG GACCTCAGGG TTTACCAGGT 
CCAATTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA 
GGACGCAAAG GAGACCCAGG TTTGCCAGGC CAGCCAGGTA CTCGTGGCTT GGATGGTCCC 
CCCGGGCCAG ATGGATTGCA GGGTCCTCCA GGTCCCCCTG GAACCTCTTC TATCGCCCAT 
GGGTTTCTCA TCACACGTCA CAGCCAGACA ACAGATGCTC CACAATGCCC ACAGGGAACC 
ATCCAGGTGT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCTCATGGT 
CAAGACTTGG GAACAGCCGG CAGCTGCCTT CGTCGTTTCA GTACCATGCC TTTCATGTTC 
TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTCTCC 
ACCCCAGAGC CCATGCCAAT GAGCATGGAA CCCCTGAAGG GGCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAAGCTCCG GCCGTGGTGA TTGCAGTCCA CAGTCAGACC 
ATCCAGATTC CCCGTTGTCC TCAGGGATGG GATTCTCTCT GGATTGGTTA TTCCTTCATG 
ATGCACACAA GTGCAGGGGC AGAGGGCTCA GGCCAAGCCC TGGCCTCCCC TGGTTCCTGC 
TTAGAAGAGT TTCGTTCAGC TCCCTTCATT GAGTGTCATG GGCGGGGGAC CTGCAACTAT 
TACGCCAACT CCTACAGCTT TTGGCTGGCG ACTGTAGATG TGGCAGACAT GTTCAGCAAA 
CCTCAATCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006072408.1
CDS	321..5378
Translation	MKLRGVSLAAGLSLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGPAGPPGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGVKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGLPGPPGLVIPRPGTGVTVGEKGNIGLPGLPGDKGDRGFPGIQGPPGLPGPPGPAVIGPPGPPGFPGERGQKGDEGPPGISIPGSPGLDGQPGAPGPPGPPGPPGPHIPPSDKICEAGPPGPPGSPGDRGLQGEQGMKGDKGDTCFNCIGTGVSGPPGQPGLPGLPGPPGSLGFPGQKGEKGHAGATGPKGLTGIPGAPGAPGFPGPKGEPGDILTFPGMKGDKGDLGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGMAFKGERGPPGNPGLPGLPGNRGPMGPVGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGHPGRDGEVGLPGEPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGSPGRIGLEGPSGPPGFPGPKGEPGFGLPGPPGPPGLPGFKGILGPKGDRGFPGPQGPPGQAGLDGLPGPKGDIGPNGQPGPIGPPGLPGTGFQGPPGPPGIPGPIGQPGLHGLPGEKGDPGPPGFDVPGPPGERGSPGIPGAPGPMGPPGSPGLPGKAGASGFPGAKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGLPGPMDPDLLGSKGEKGDPGLPGIPGVAGPKGYQGLPGDPGQPGLSGQPGLPGPAGPKGNPGLPGMPGLTGPPGLKGNMGDMGFPGPQGPKGSFGPPGVPGQPGSPGLPGQKGEKGDPGISGIGLPGLPGPKGEPGLPGYPGNPGTKGSMGETGLPGLPGTPGAKGQPGLPGFPGTPGLPGPKGISGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDEGLPGIPGNPGLPGPKGEPGFHGFPGLQGPPGPPGSPGPALEGPKGSPGPQGPPGRPGPPGPEGPRGLPGSGGIKGERGNPGQPGQPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSTGPEGDPGLIGPPGPPGLPGPSGQSIVIKGDAGPPGIPGQPGLKGLQGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTIQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVADMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_006072346.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen alpha-5(IV) chain-like (LOC102399306), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006072346.1

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTCCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CTATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGC CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC CAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAACCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCACCAGG TCCAGAAGGT CCCCGGGGTC TACCTGGAAG TGGGGGTATT 
AAAGGAGAAA GAGGTAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG TTTGAAAGGT 
GATCAAGGTC CTCCAGGACT CCAGGGTAAC CCTGGCCGGC CAGGTCTCAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGCA TGAAAGGACC CAGTGGAGTA 
CCTGGTTCAA CTGGCCCTGA GGGGGATCCA GGACTTATTG GCCCACCAGG TCCCCCTGGA 
TTACCTGGTC CTTCAGGTCA GAGTATTGTA ATCAAAGGAG ATGCTGGCCC ACCAGGGATT 
CCAGGCCAGC CTGGATTAAA AGGTTTACAA GGACTCCCAG GACCTCAGGG TTTACCAGGT 
CCAATTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA 
GGACGCAAAG GAGACCCAGG TTTGCCAGGC CAGCCAGGTA CTCGTGGCTT GGATGGTCCC 
CCCGGGCCAG ATGGATTGCA GGGTCCTCCA GGTCCCCCTG GAACCTCTTC TATCGCCCAT 
GGGTTTCTCA TCACACGTCA CAGCCAGACA ACAGATGCTC CACAATGCCC ACAGGGAACC 
ATCCAGGTGT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCTCATGGT 
CAAGACTTGG GAACAGCCGG CAGCTGCCTT CGTCGTTTCA GTACCATGCC TTTCATGTTC 
TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTCTCC 
ACCCCAGAGC CCATGCCAAT GAGCATGGAA CCCCTGAAGG GGCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAAGCTCCG GCCGTGGTGA TTGCAGTCCA CAGTCAGACC 
ATCCAGATTC CCCGTTGTCC TCAGGGATGG GATTCTCTCT GGATTGGTTA TTCCTTCATG 
ATGCACACAA GTGCAGGGGC AGAGGGCTCA GGCCAAGCCC TGGCCTCCCC TGGTTCCTGC 
TTAGAAGAGT TTCGTTCAGC TCCCTTCATT GAGTGTCATG GGCGGGGGAC CTGCAACTAT 
TACGCCAACT CCTACAGCTT TTGGCTGGCG ACTGTAGATG TGGCAGACAT GTTCAGCAAA 
CCTCAATCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102399306 ( XM_006072346.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006072408.1 Bubalus bubalis collagen type IV alpha 5 chain (COL4A5), transcript variant X6, mRNA.

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CloneID	OBu26606
Clone ID Related Accession (Same CDS sequence)	XM_006072346.1 , XM_006072346.2
Accession Version	XM_006072346.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-5(IV) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006072346.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTCCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CTATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGC CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC CAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAACCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCACCAGG TCCAGAAGGT CCCCGGGGTC TACCTGGAAG TGGGGGTATT 
AAAGGAGAAA GAGGTAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG TTTGAAAGGT 
GATCAAGGTC CTCCAGGACT CCAGGGTAAC CCTGGCCGGC CAGGTCTCAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGCA TGAAAGGACC CAGTGGAGTA 
CCTGGTTCAA CTGGCCCTGA GGGGGATCCA GGACTTATTG GCCCACCAGG TCCCCCTGGA 
TTACCTGGTC CTTCAGGTCA GAGTATTGTA ATCAAAGGAG ATGCTGGCCC ACCAGGGATT 
CCAGGCCAGC CTGGATTAAA AGGTTTACAA GGACTCCCAG GACCTCAGGG TTTACCAGGT 
CCAATTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA 
GGACGCAAAG GAGACCCAGG TTTGCCAGGC CAGCCAGGTA CTCGTGGCTT GGATGGTCCC 
CCCGGGCCAG ATGGATTGCA GGGTCCTCCA GGTCCCCCTG GAACCTCTTC TATCGCCCAT 
GGGTTTCTCA TCACACGTCA CAGCCAGACA ACAGATGCTC CACAATGCCC ACAGGGAACC 
ATCCAGGTGT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCTCATGGT 
CAAGACTTGG GAACAGCCGG CAGCTGCCTT CGTCGTTTCA GTACCATGCC TTTCATGTTC 
TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTCTCC 
ACCCCAGAGC CCATGCCAAT GAGCATGGAA CCCCTGAAGG GGCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAAGCTCCG GCCGTGGTGA TTGCAGTCCA CAGTCAGACC 
ATCCAGATTC CCCGTTGTCC TCAGGGATGG GATTCTCTCT GGATTGGTTA TTCCTTCATG 
ATGCACACAA GTGCAGGGGC AGAGGGCTCA GGCCAAGCCC TGGCCTCCCC TGGTTCCTGC 
TTAGAAGAGT TTCGTTCAGC TCCCTTCATT GAGTGTCATG GGCGGGGGAC CTGCAACTAT 
TACGCCAACT CCTACAGCTT TTGGCTGGCG ACTGTAGATG TGGCAGACAT GTTCAGCAAA 
CCTCAATCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006072408.1
CDS	328..5385
Translation	MKLRGVSLAAGLSLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGPAGPPGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGVKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGLPGPPGLVIPRPGTGVTVGEKGNIGLPGLPGDKGDRGFPGIQGPPGLPGPPGPAVIGPPGPPGFPGERGQKGDEGPPGISIPGSPGLDGQPGAPGPPGPPGPPGPHIPPSDKICEAGPPGPPGSPGDRGLQGEQGMKGDKGDTCFNCIGTGVSGPPGQPGLPGLPGPPGSLGFPGQKGEKGHAGATGPKGLTGIPGAPGAPGFPGPKGEPGDILTFPGMKGDKGDLGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGMAFKGERGPPGNPGLPGLPGNRGPMGPVGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGHPGRDGEVGLPGEPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGSPGRIGLEGPSGPPGFPGPKGEPGFGLPGPPGPPGLPGFKGILGPKGDRGFPGPQGPPGQAGLDGLPGPKGDIGPNGQPGPIGPPGLPGTGFQGPPGPPGIPGPIGQPGLHGLPGEKGDPGPPGFDVPGPPGERGSPGIPGAPGPMGPPGSPGLPGKAGASGFPGAKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGLPGPMDPDLLGSKGEKGDPGLPGIPGVAGPKGYQGLPGDPGQPGLSGQPGLPGPAGPKGNPGLPGMPGLTGPPGLKGNMGDMGFPGPQGPKGSFGPPGVPGQPGSPGLPGQKGEKGDPGISGIGLPGLPGPKGEPGLPGYPGNPGTKGSMGETGLPGLPGTPGAKGQPGLPGFPGTPGLPGPKGISGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDEGLPGIPGNPGLPGPKGEPGFHGFPGLQGPPGPPGSPGPALEGPKGSPGPQGPPGRPGPPGPEGPRGLPGSGGIKGERGNPGQPGQPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSTGPEGDPGLIGPPGPPGLPGPSGQSIVIKGDAGPPGIPGQPGLKGLQGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTIQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVADMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_006072346.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen type IV alpha 5 chain (COL4A5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006072346.2

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCTAA GTGTGACTGT 
AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT 
TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCAGGTAGT 
ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA 
ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGCAA TATGGGCTTA 
AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA 
CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT 
CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG 
CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC 
ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT 
CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC 
TTTCCTGGGG AAAGAGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC 
GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA 
TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC 
TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT 
CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT 
GGTGCAACTG GTCCCAAAGG ATTAACAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGACCTA AAGGTGAACC TGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA 
GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG 
GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG 
GGCCCTGTTG GTTTTGGCCC TCCAGGTCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC 
CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGAACCTG GACTCCCGGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA 
CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG 
CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG 
CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA 
GGACCTCAGG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC 
ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT 
CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA 
CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTCCAGG ACCTCCTGGT 
GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA 
GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT 
CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CTATGGATCC AGATCTGCTG 
GGCTCAAAAG GAGAGAAAGG GGACCCTGGC CTACCAGGTA TTCCTGGAGT TGCAGGGCCA 
AAAGGTTACC AGGGTTTACC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTGCCAGGAC CAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA 
GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA 
AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC 
CCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG 
GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA 
GGGCCTCCAG GTGAAAAAGG CAAACCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT 
CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT 
GGGAGACCAG GTCCACCAGG TCCAGAAGGT CCCCGGGGTC TACCTGGAAG TGGGGGTATT 
AAAGGAGAAA GAGGTAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG TTTGAAAGGT 
GATCAAGGTC CTCCAGGACT CCAGGGTAAC CCTGGCCGGC CAGGTCTCAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGCA TGAAAGGACC CAGTGGAGTA 
CCTGGTTCAA CTGGCCCTGA GGGGGATCCA GGACTTATTG GCCCACCAGG TCCCCCTGGA 
TTACCTGGTC CTTCAGGTCA GAGTATTGTA ATCAAAGGAG ATGCTGGCCC ACCAGGGATT 
CCAGGCCAGC CTGGATTAAA AGGTTTACAA GGACTCCCAG GACCTCAGGG TTTACCAGGT 
CCAATTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA 
GGACGCAAAG GAGACCCAGG TTTGCCAGGC CAGCCAGGTA CTCGTGGCTT GGATGGTCCC 
CCCGGGCCAG ATGGATTGCA GGGTCCTCCA GGTCCCCCTG GAACCTCTTC TATCGCCCAT 
GGGTTTCTCA TCACACGTCA CAGCCAGACA ACAGATGCTC CACAATGCCC ACAGGGAACC 
ATCCAGGTGT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCTCATGGT 
CAAGACTTGG GAACAGCCGG CAGCTGCCTT CGTCGTTTCA GTACCATGCC TTTCATGTTC 
TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTCTCC 
ACCCCAGAGC CCATGCCAAT GAGCATGGAA CCCCTGAAGG GGCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAAGCTCCG GCCGTGGTGA TTGCAGTCCA CAGTCAGACC 
ATCCAGATTC CCCGTTGTCC TCAGGGATGG GATTCTCTCT GGATTGGTTA TTCCTTCATG 
ATGCACACAA GTGCAGGGGC AGAGGGCTCA GGCCAAGCCC TGGCCTCCCC TGGTTCCTGC 
TTAGAAGAGT TTCGTTCAGC TCCCTTCATT GAGTGTCATG GGCGGGGGAC CTGCAACTAT 
TACGCCAACT CCTACAGCTT TTGGCTGGCG ACTGTAGATG TGGCAGACAT GTTCAGCAAA 
CCTCAATCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC102399306 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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