HECW2 gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006078133.1	XP_006078195.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_006078134.1	XP_006078196.1	E3 ubiquitin-protein ligase HECW2 isoform X2
XM_006078135.2	XP_006078197.2	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_006078136.1	XP_006078198.1	E3 ubiquitin-protein ligase HECW2 isoform X4
XM_006078137.1	XP_006078199.1	E3 ubiquitin-protein ligase HECW2 isoform X5
XM_006078136.2	XP_006078198.2	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_025277891.1	XP_025133676.1	E3 ubiquitin-protein ligase HECW2 isoform X4
XM_025277884.1	XP_025133669.1	E3 ubiquitin-protein ligase HECW2 isoform X2
XM_006078137.2	XP_006078199.2	E3 ubiquitin-protein ligase HECW2 isoform X3
XM_006078135.1	XP_006078197.1	E3 ubiquitin-protein ligase HECW2 isoform X3
XM_025277874.1	XP_025133659.1	E3 ubiquitin-protein ligase HECW2 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu31386	XM_006078137.1 Latest version!	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu55715	XM_006078137.2 Latest version!	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu55716	XM_025277891.1 Latest version!	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu31385	XM_006078135.1 Latest version!	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu31385	XM_006078134.1 Latest version!	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu31385	XM_006078133.1 Latest version!	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu31385	XM_006078136.1 Latest version!	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu55713	XM_025277874.1 Latest version!	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu55713	XM_006078136.2 Latest version!	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu55713	XM_006078135.2 Latest version!	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu55714	XM_025277884.1 Latest version!	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

HECW2 ( XM_006078137.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006078199.1 Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu31386
Clone ID Related Accession (Same CDS sequence)	XM_006078137.1
Accession Version	XM_006078137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase HECW2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785813.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG AGATAAAAAT CTGTTTCAAA TACTACCACG GCATCAGTGG AGCCCTGCGA 
GCCACGACCC CTTGCATCAC GGTGAAGAAC CCAGCTGTGA TGATGGGGGC AGAAGGCATG 
GAAGGAGGGG CTTCAGGAAA TCAGCATTCT CGGAAGCTTG TTAGCTTTAC TTTGTCAGAC 
CTTAGGGCAG TTGGGCTAAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG 
TCCATTCAGC CAGGGAAGAA GAGCAGTTTC CCCACCTGTG CCCACCATGG GCAGGAGAGG 
AGGTCTACTA TCATCAGTAA CACCACTAAT CCAGTTTGGC ACCGAGAGAA ATACTCCTTT 
TTTGCACTCC TTACTGATGT CTTAGAGATT GAAATTAAAG ACAAGTTTGC TAAGAGCCGG 
CCTATCATTA AGCGTTTCCT GGGGAAGCTC ACCATTCCAG TCCAGAGGCT ACTGGAGCGG 
CAAGCCATTG GTGACCAGAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC AGCTGACCAT 
GTGAGTGGGT ACCTCCAGTT CAGAGTGGAG GTCACATCTT CTATTCATGA AGATGCCTCT 
CCAGAAGCTG TTGGCACAAT ACTTGGAGTC AGTTCTGTGA ACGGAGACCT AGGAAGCCCT 
TCTGATGAGG AGGACATGCC AGGGGGCCAT CACGACAGCC CAGTTTGTTC CAATGGACCA 
GTTTCTGAGG AAAGTGCTGA TGGGACACCC AAGCATTCTC TCAGGACTAG TTCTACTCTG 
GAAATAGACA CAGAAGAATT AACTTCTACT TCTTCAAGGA CCTCACCTCC CAGAGGACGT 
CAGGATTCAC TCAATGATTA TTTAGATGCT ATTGAACACA GTGGCCATTC TAGGTCAGGT 
GCAGCTACTT GCTCGGAGAG GTCCGTGGGA GCCTCTCCAA AGTTAAGGAG TAGTTTTCCC 
ACTGATACAA GACTCAATGC AATGCTTCAT ATTGACTCAG ATGAAGAAGA CCACGAGTTC 
CAGCAAGACC TGGGCTATCC ATCCTCCTTG GAGGAGGAAG GAGGGTTGAT CATGTTTAGC 
AGGGCATCAA GAACTGATGA TGGGAGCCTG GCATCTCAGA CAAAGCCAGA AGACAACCCA 
GTGGAGACTG AGGAAGCCTC CATTAATGAA GCTGCTTCCT TTGAGGATAA GCCAGGAAAT 
CTTCCAGAGC TTGCAGAGAC CTCTTTACCC TCAGGCCCGG TCCCAGATGA AAGTGAGAAT 
GGCCCAGAAT CTCAACCAAG TGTGGACCAG GGCAGCACTG AATTGTGTGG CTCTCAGGAG 
GTGGATCAGC CCACAAGTGG AGCTGACACG GGCACTTCTG ACACGTCTGG GGGGAGCCGA 
AGAGCTGTCA GTGAGACAGA GTCCCTCGAT CAGGGGTCTG AGCCTTCCCA GGTGTCCTCA 
GAAACAGAGC CCAGCGACCC TGCCAGGACA GAGAGCGTGA GCGAGGCCAG CACCAGGCCC 
GAGGGAGAGA GTGACCTGGA AGGGGCCGAC AGCTCCTGCA ATGAGAGTGT TACCACGCAG 
CTCTCCTCGG TGGAGACACG GTGCTCATCT CTTGAGAGCG CACGGTTTCC TGAAACTCCA 
GCCTTTTCTT CTCAGGAGGA GGAAGATGGA GCCTGTGCAG CAGAGCCCGC CAGCAGTGGC 
CCTGCTGAAG GGTCCCAGGA TTCCATATGC ACTGCTGGTT CCTTACCTGT GGTGCAGGTG 
CCCAGTGGAG AGGAGGAAGG GCCAGGGTCA GAATCAACCA CTGTACCTGA CCAGGAGGAG 
GTGGGGGAGG TCTGGCAGCG GAGGGGGAGC CTGGAGGGAG CTGCTGCTGC TGCTGAGAGC 
CAGCCCCAAG CAGAGGGTGC TGCTCGGGAT ACCCCAGGGG CTTGTGAAGG GGCCACCGCC 
CAGGAGGAGG GCGCGATTGG AGGTTGTCAA TCCAACGGCC ACCAGCCACT GCGATCGCTG 
CCTTCAGTTC GCCAGGATGT TAGCCGGTAC CAGAGGGTGG ACGAGGCTCT CCCACCAAAC 
TGGGAGGCGC GCATTGACAG CCACGGCAGG ATCTTCTACG TGGATCACGT AAACAGAACC 
ACGACGTGGC AGCGGCCCAC AGCTCCCCCT GCCCCGCAGG TGCTGCAGAG GTCCAACTCC 
ATACAGCAGA TGGAGCAGCT GAACCGGCGA TACCAGAGTA TCCGCAGAAC CATGACCAAC 
GAGAGGCCTG AGGAAAATAG CAATGCTATT GATGGGGCCG GGGAGGAAGC CGACTTTCAC 
CAAGCCAGTG CAGATTTCCG ACGGGAGAGC GTCCTGCCCC ACTCTACCTC CAGATCCAGG 
CTCACGTTGC TGTTACAGTC TCCCCCCGTG AAGTTCCTCA TCAGCCCAGA GTTCTTCACC 
GTGCTGCATT CCAACCCTAG TGCCTACCGC ATGTTTACAA ACAACACATG TTTGAAGCAC 
ATGATCACCA AAGTCCGGCG GGACACCCAC CACTTTGAAC GCTACCAGCA TAACCGGGAC 
CTCGTGGGAT TCCTCAACAT GTTTGCAAAC AAACAACTGG AGCTGCCAAG GGGCTGGGAA 
ATGAAACACG ATCATCAGGG CAAGGCATTT TTTGTTGACC ACAACTCCCG AACCACCACT 
TTCATCGATC CCCGACTCCC GCTTCAGAGC AGCAGACCCA CGAGTGCACT GGTGCATCGA 
CAACACCTGA CCCGGCAGCG CAGCCACAGC GCAGGCGAGG TAGGAGAAGA TTCTCGACAT 
GCAGGACCAC CAGTTCTTCC CAGGCCGTCG AGTACGTTCA ATACAGTCAG CAGGCCGCAG 
TACCAGGACA TGGTTCCAGT GGCTTACAAT GACAAGATTG TTGCATTTCT GCGCCAGCCC 
AACATCTTTG AAATTCTGCA GGAACGTCAA CCTGATCTTA CCAGGAATCA CTCACTCAGG 
GAGAAGATCC AATTTATCCG AACTGAAGGG ACTCCAGGAT TGGTGCGTCT TTCAAGTGAT 
GCAGACCTTG TCATGCTGCT GAGTTTATTT GAAGAAGAGA TAATGTCATA TGTGCCTCCA 
CATGCCTTAC TCCATCCCAG CTACTGTCAG TCCCCACGTG GCTCCCCTGT GTCCTCTCCC 
CAGAACTCAC CAGGTACTCA GCGTGCCAAT GCCCGCGCTC CAGCCCCTTA CAAGCGAGAC 
TTTGAAGCCA AACTAAGGAA CTTTTACAGG AAGTTAGAGA CTAAAGGATA TGGACAAGGC 
CCAGGGAAAT TAAAGTTAAT TATCAGAAGA GATCACTTGC TTGAAGATGC TTTTAATCAA 
ATTATGGGCT ACTCCAGAAA AGACCTGCAG AGAAATAAGC TGTACGTCAC ATTCGTTGGA 
GAGGAAGGGC TGGATTACAG TGGACCTTCC AGAGAGTTTT TCTTCCTGGT ATCCAGAGAA 
CTCTTTAACC CATATTATGG CTTATTTGAA TATTCAGCCA ATGACACATA CACAGTACAA 
ATAAGTCCCA TGTCTGCCTT TGTAGACAAT CACCATGAAT GGTTCCGGTT CAGTGGTAGG 
ATCCTTGGTC TTGCACTAAT TCACCAGTAT TTGTTGGATG CCTTCTTCAC ACGGCCATTT 
TATAAGGCTC TTCTCAGAAT TCTCTGTGAC CTGAGTGATC TAGAATACCT TGATGAAGAG 
TTCCATCAGA GCCTGCAGTG GATGAAAGAC AACGACATCC ATGACATCCT GGACCTCACG 
TTCACTGTGA ACGAAGAAGT TTTTGGGCAG ATTACTGAAC GAGAATTAAA ACCAGGGGGT 
GCCAACATCC CAGTCACAGA GAAGAACAAG AAGGAGTACA TTGAGAGGAT GGTGAAATGG 
AGGATTGAGA GGGGTGTCGT GCAGCAGACA GAGAGCTTAG TGCGTGGCTT CTATGAGGTG 
GTGGATGCCA GGCTGGTATC TGTTTTTGAT GCAAGAGAAC TAGAACTGGT CATCGCAGGC 
ACAGCTGAAA TAGACCTAAG TGACTGGAGA AACAACACAG AATACAGAGG AGGATATCAT 
GACAATCACA TTGTAATTCG GTGGTTCTGG GCTGCAGTGG AAAGATTCAA CAATGAACAA 
CGACTGAGGT TGTTACAGTT TGTTACAGGC ACATCCAGCA TTCCCTATGA AGGATTTGCT 
TCTCTCCGAG GCAGTAATGG CCCAAGAAGA TTCTGTGTGG AGAAATGGGG GAAAATCACT 
GCTCTTCCCA GAGCTCACAC TTGTTTTAAC CGTCTGGATC TCCCTCCATA CCCATCCTTC 
TCCATGCTTT ATGAAAAACT CTTGACAGCA GTTGAAGAGA CCAGTACCTT TGGACTTGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006078199.1
CDS	449..4771
Translation	MEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPVWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSIHEDASPEAVGTILGVSSVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHSGHSRSGAATCSERSVGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLASQTKPEDNPVETEEASINEAASFEDKPGNLPELAETSLPSGPVPDESENGPESQPSVDQGSTELCGSQEVDQPTSGADTGTSDTSGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPASSGPAEGSQDSICTAGSLPVVQVPSGEEEGPGSESTTVPDQEEVGEVWQRRGSLEGAAAAAESQPQAEGAARDTPGACEGATAQEEGAIGGCQSNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENSNAIDGAGEEADFHQASADFRRESVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006078137.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006078137.1

ORF Insert Sequence:

ATGGAGCCCG AGATAAAAAT CTGTTTCAAA TACTACCACG GCATCAGTGG AGCCCTGCGA 
GCCACGACCC CTTGCATCAC GGTGAAGAAC CCAGCTGTGA TGATGGGGGC AGAAGGCATG 
GAAGGAGGGG CTTCAGGAAA TCAGCATTCT CGGAAGCTTG TTAGCTTTAC TTTGTCAGAC 
CTTAGGGCAG TTGGGCTAAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG 
TCCATTCAGC CAGGGAAGAA GAGCAGTTTC CCCACCTGTG CCCACCATGG GCAGGAGAGG 
AGGTCTACTA TCATCAGTAA CACCACTAAT CCAGTTTGGC ACCGAGAGAA ATACTCCTTT 
TTTGCACTCC TTACTGATGT CTTAGAGATT GAAATTAAAG ACAAGTTTGC TAAGAGCCGG 
CCTATCATTA AGCGTTTCCT GGGGAAGCTC ACCATTCCAG TCCAGAGGCT ACTGGAGCGG 
CAAGCCATTG GTGACCAGAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC AGCTGACCAT 
GTGAGTGGGT ACCTCCAGTT CAGAGTGGAG GTCACATCTT CTATTCATGA AGATGCCTCT 
CCAGAAGCTG TTGGCACAAT ACTTGGAGTC AGTTCTGTGA ACGGAGACCT AGGAAGCCCT 
TCTGATGAGG AGGACATGCC AGGGGGCCAT CACGACAGCC CAGTTTGTTC CAATGGACCA 
GTTTCTGAGG AAAGTGCTGA TGGGACACCC AAGCATTCTC TCAGGACTAG TTCTACTCTG 
GAAATAGACA CAGAAGAATT AACTTCTACT TCTTCAAGGA CCTCACCTCC CAGAGGACGT 
CAGGATTCAC TCAATGATTA TTTAGATGCT ATTGAACACA GTGGCCATTC TAGGTCAGGT 
GCAGCTACTT GCTCGGAGAG GTCCGTGGGA GCCTCTCCAA AGTTAAGGAG TAGTTTTCCC 
ACTGATACAA GACTCAATGC AATGCTTCAT ATTGACTCAG ATGAAGAAGA CCACGAGTTC 
CAGCAAGACC TGGGCTATCC ATCCTCCTTG GAGGAGGAAG GAGGGTTGAT CATGTTTAGC 
AGGGCATCAA GAACTGATGA TGGGAGCCTG GCATCTCAGA CAAAGCCAGA AGACAACCCA 
GTGGAGACTG AGGAAGCCTC CATTAATGAA GCTGCTTCCT TTGAGGATAA GCCAGGAAAT 
CTTCCAGAGC TTGCAGAGAC CTCTTTACCC TCAGGCCCGG TCCCAGATGA AAGTGAGAAT 
GGCCCAGAAT CTCAACCAAG TGTGGACCAG GGCAGCACTG AATTGTGTGG CTCTCAGGAG 
GTGGATCAGC CCACAAGTGG AGCTGACACG GGCACTTCTG ACACGTCTGG GGGGAGCCGA 
AGAGCTGTCA GTGAGACAGA GTCCCTCGAT CAGGGGTCTG AGCCTTCCCA GGTGTCCTCA 
GAAACAGAGC CCAGCGACCC TGCCAGGACA GAGAGCGTGA GCGAGGCCAG CACCAGGCCC 
GAGGGAGAGA GTGACCTGGA AGGGGCCGAC AGCTCCTGCA ATGAGAGTGT TACCACGCAG 
CTCTCCTCGG TGGAGACACG GTGCTCATCT CTTGAGAGCG CACGGTTTCC TGAAACTCCA 
GCCTTTTCTT CTCAGGAGGA GGAAGATGGA GCCTGTGCAG CAGAGCCCGC CAGCAGTGGC 
CCTGCTGAAG GGTCCCAGGA TTCCATATGC ACTGCTGGTT CCTTACCTGT GGTGCAGGTG 
CCCAGTGGAG AGGAGGAAGG GCCAGGGTCA GAATCAACCA CTGTACCTGA CCAGGAGGAG 
GTGGGGGAGG TCTGGCAGCG GAGGGGGAGC CTGGAGGGAG CTGCTGCTGC TGCTGAGAGC 
CAGCCCCAAG CAGAGGGTGC TGCTCGGGAT ACCCCAGGGG CTTGTGAAGG GGCCACCGCC 
CAGGAGGAGG GCGCGATTGG AGGTTGTCAA TCCAACGGCC ACCAGCCACT GCGATCGCTG 
CCTTCAGTTC GCCAGGATGT TAGCCGGTAC CAGAGGGTGG ACGAGGCTCT CCCACCAAAC 
TGGGAGGCGC GCATTGACAG CCACGGCAGG ATCTTCTACG TGGATCACGT AAACAGAACC 
ACGACGTGGC AGCGGCCCAC AGCTCCCCCT GCCCCGCAGG TGCTGCAGAG GTCCAACTCC 
ATACAGCAGA TGGAGCAGCT GAACCGGCGA TACCAGAGTA TCCGCAGAAC CATGACCAAC 
GAGAGGCCTG AGGAAAATAG CAATGCTATT GATGGGGCCG GGGAGGAAGC CGACTTTCAC 
CAAGCCAGTG CAGATTTCCG ACGGGAGAGC GTCCTGCCCC ACTCTACCTC CAGATCCAGG 
CTCACGTTGC TGTTACAGTC TCCCCCCGTG AAGTTCCTCA TCAGCCCAGA GTTCTTCACC 
GTGCTGCATT CCAACCCTAG TGCCTACCGC ATGTTTACAA ACAACACATG TTTGAAGCAC 
ATGATCACCA AAGTCCGGCG GGACACCCAC CACTTTGAAC GCTACCAGCA TAACCGGGAC 
CTCGTGGGAT TCCTCAACAT GTTTGCAAAC AAACAACTGG AGCTGCCAAG GGGCTGGGAA 
ATGAAACACG ATCATCAGGG CAAGGCATTT TTTGTTGACC ACAACTCCCG AACCACCACT 
TTCATCGATC CCCGACTCCC GCTTCAGAGC AGCAGACCCA CGAGTGCACT GGTGCATCGA 
CAACACCTGA CCCGGCAGCG CAGCCACAGC GCAGGCGAGG TAGGAGAAGA TTCTCGACAT 
GCAGGACCAC CAGTTCTTCC CAGGCCGTCG AGTACGTTCA ATACAGTCAG CAGGCCGCAG 
TACCAGGACA TGGTTCCAGT GGCTTACAAT GACAAGATTG TTGCATTTCT GCGCCAGCCC 
AACATCTTTG AAATTCTGCA GGAACGTCAA CCTGATCTTA CCAGGAATCA CTCACTCAGG 
GAGAAGATCC AATTTATCCG AACTGAAGGG ACTCCAGGAT TGGTGCGTCT TTCAAGTGAT 
GCAGACCTTG TCATGCTGCT GAGTTTATTT GAAGAAGAGA TAATGTCATA TGTGCCTCCA 
CATGCCTTAC TCCATCCCAG CTACTGTCAG TCCCCACGTG GCTCCCCTGT GTCCTCTCCC 
CAGAACTCAC CAGGTACTCA GCGTGCCAAT GCCCGCGCTC CAGCCCCTTA CAAGCGAGAC 
TTTGAAGCCA AACTAAGGAA CTTTTACAGG AAGTTAGAGA CTAAAGGATA TGGACAAGGC 
CCAGGGAAAT TAAAGTTAAT TATCAGAAGA GATCACTTGC TTGAAGATGC TTTTAATCAA 
ATTATGGGCT ACTCCAGAAA AGACCTGCAG AGAAATAAGC TGTACGTCAC ATTCGTTGGA 
GAGGAAGGGC TGGATTACAG TGGACCTTCC AGAGAGTTTT TCTTCCTGGT ATCCAGAGAA 
CTCTTTAACC CATATTATGG CTTATTTGAA TATTCAGCCA ATGACACATA CACAGTACAA 
ATAAGTCCCA TGTCTGCCTT TGTAGACAAT CACCATGAAT GGTTCCGGTT CAGTGGTAGG 
ATCCTTGGTC TTGCACTAAT TCACCAGTAT TTGTTGGATG CCTTCTTCAC ACGGCCATTT 
TATAAGGCTC TTCTCAGAAT TCTCTGTGAC CTGAGTGATC TAGAATACCT TGATGAAGAG 
TTCCATCAGA GCCTGCAGTG GATGAAAGAC AACGACATCC ATGACATCCT GGACCTCACG 
TTCACTGTGA ACGAAGAAGT TTTTGGGCAG ATTACTGAAC GAGAATTAAA ACCAGGGGGT 
GCCAACATCC CAGTCACAGA GAAGAACAAG AAGGAGTACA TTGAGAGGAT GGTGAAATGG 
AGGATTGAGA GGGGTGTCGT GCAGCAGACA GAGAGCTTAG TGCGTGGCTT CTATGAGGTG 
GTGGATGCCA GGCTGGTATC TGTTTTTGAT GCAAGAGAAC TAGAACTGGT CATCGCAGGC 
ACAGCTGAAA TAGACCTAAG TGACTGGAGA AACAACACAG AATACAGAGG AGGATATCAT 
GACAATCACA TTGTAATTCG GTGGTTCTGG GCTGCAGTGG AAAGATTCAA CAATGAACAA 
CGACTGAGGT TGTTACAGTT TGTTACAGGC ACATCCAGCA TTCCCTATGA AGGATTTGCT 
TCTCTCCGAG GCAGTAATGG CCCAAGAAGA TTCTGTGTGG AGAAATGGGG GAAAATCACT 
GCTCTTCCCA GAGCTCACAC TTGTTTTAAC CGTCTGGATC TCCCTCCATA CCCATCCTTC 
TCCATGCTTT ATGAAAAACT CTTGACAGCA GTTGAAGAGA CCAGTACCTT TGGACTTGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_006078137.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006078199.2 Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu55715
Clone ID Related Accession (Same CDS sequence)	XM_006078137.2
Accession Version	XM_006078137.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase HECW2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006078137.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG AGATAAAAAT CTGTTTCAAA TACTACCATG GCATCAGTGG AGCCCTGCGA 
GCCACGACCC CTTGCATCAC GGTGAAGAAC CCAGCTGTGA TGATGGGGGC AGAAGGCATG 
GAAGGAGGGG CTTCAGGAAA TCAGCATTCT CGGAAGCTTG TTAGCTTTAC TTTGTCAGAC 
CTTAGGGCAG TTGGGCTAAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG 
TCCATTCAGC CAGGGAAGAA GAGCAGTTTC CCCACCTGTG CCCACCATGG GCAGGAGAGG 
AGGTCTACTA TCATCAGTAA CACCACTAAT CCAGTTTGGC ACCGAGAGAA ATACTCCTTT 
TTTGCACTCC TTACTGATGT CTTAGAGATT GAAATTAAAG ACAAGTTTGC TAAGAGCCGG 
CCTATCATTA AGCGTTTCCT GGGGAAGCTC ACCATTCCAG TCCAGAGGCT ACTGGAGCGG 
CAAGCCATTG GTGACCAGAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC AGCTGACCAT 
GTGAGTGGGT ACCTCCAGTT CAGAGTGGAG GTCACATCTT CTATTCATGA AGATGCCTCT 
CCAGAAGCTG TTGGCACAAT ACTTGGAGTC AGTTCTGTGA ACGGAGACCT AGGAAGCCCT 
TCTGATGAGG AGGACATGCC AGGGGGCCAT CACGACAGCC CAGTTTGTTC CAATGGACCA 
GTTTCTGAGG AAAGTGCTGA TGGGACACCC AAGCATTCTC TCAGGACTAG TTCTACTCTG 
GAAATAGACA CAGAAGAATT AACTTCTACT TCTTCAAGGA CCTCACCTCC CAGAGGACGT 
CAGGATTCAC TCAATGATTA TTTAGATGCT ATTGAACACA GTGGCCATTC TAGGTCAGGT 
GCAGCTACTT GCTCGGAGAG GTCCGTGGGA GCCTCTCCAA AGTTAAGGAG TAGTTTTCCC 
ACTGATACAA GACTCAATGC AATGCTTCAT ATTGACTCAG ATGAAGAAGA CCACGAGTTC 
CAGCAAGACC TGGGCTATCC ATCCTCCTTG GAGGAGGAAG GAGGGTTGAT CATGTTTAGC 
AGGGCATCAA GAACTGATGA TGGGAGCCTG GCATCTCAGA CAAAGCCAGA AGACAACCCA 
GTGGAGACTG AGGAAGCCTC CATTAATGAA GCTGCTTCCT TTGAGGATAA GCCAGGAAAT 
CTTCCAGAGC TTGCAGAGAC CTCTTTACCC TCAGGCCCGG TCCCAGATGA AAGTGAGAAT 
GGCCCAGAAT CTCAACCAAG TGTGGACCAG GGCAGCACTG AATTGTGTGG CTCTCAGGAG 
GTGGATCAGC CCACAAGTGG AGCTGACACG GGCACTTCTG ACACGTCTGG GGGGAGCCGA 
AGAGCTGTCA GTGAGACAGA GTCCCTCGAT CAGGGGTCTG AGCCTTCCCA GGTGTCCTCA 
GAAACAGAGC CCAGCGACCC TGCCAGGACA GAGAGTGTGA GCGAGGCCAG CACCAGGCCC 
GAGGGAGAGA GTGACCTGGA AGGGGCCGAC AGCTCCTGCA ATGAGAGTGT TACCACGCAG 
CTCTCCTCGG TGGAGACACG GTGCTCATCT CTTGAGAGCG CACGGTTTCC TGAAACTCCA 
GCCTTTTCTT CTCAGGAGGA GGAAGATGGA GCCTGTGCAG CAGAGCCCAC CAGCAGTGGC 
CCTGCTGAAG GGTCCCAGGA TTCCATATGC ACTGCTGGTT CCTTACCTGT GGTGCAGGTG 
CCCAGTGGAG AGGAGGAAGG GCCAGGGTCA GAATCAACCA CTGTACCTGA CCAGGAGGAG 
GTGGGGGAGG TCTGGCAGCG GAGGGGGAGC CTGGAGGGAG CTGCTGCTGC TGCTGAGAGC 
CAGCCCCAAG CAGAAGGTGC TGCTCGGGAT ACCCCAGGGG CTTGTGAAGG GGCCACCGCC 
CAGGAGGAGG GCGCGATTGG AGGTTGTCAA TCCAACGGCC ACCAGCCACT GCGATCGCTG 
CCTTCAGTTC GCCAGGATGT TAGCCGGTAC CAGAGGGTGG ACGAGGCTCT CCCACCAAAC 
TGGGAGGCGC GCATTGACAG CCACGGCAGG ATCTTCTACG TGGATCACGT AAACAGAACC 
ACGACGTGGC AGCGGCCCAC AGCTCCCCCT GCCCCGCAGG TGCTGCAGAG GTCCAACTCC 
ATACAGCAGA TGGAGCAGCT GAACCGGCGA TACCAGAGTA TCCGCAGAAC CATGACCAAC 
GAGAGGCCTG AGGAAAATAG CAATGCTATT GATGGGGCCG GGGAGGAAGC CGACTTTCAC 
CAAGCCAGTG CAGATTTCCG ACGGGAGAGC GTCCTGCCCC ACTCTACCTC CAGATCCAGG 
CTCACGTTGC TGTTACAGTC TCCCCCCGTG AAGTTCCTCA TCAGCCCAGA GTTCTTCACC 
GTGCTGCATT CCAACCCTAG TGCCTACCGC ATGTTTACAA ACAACACATG TTTGAAGCAC 
ATGATCACCA AAGTCCGGCG GGACACCCAC CACTTTGAAC GCTACCAGCA TAACCGGGAC 
CTCGTGGGAT TCCTCAACAT GTTTGCAAAC AAACAACTGG AGCTGCCAAG GGGCTGGGAA 
ATGAAACACG ATCATCAGGG CAAGGCATTT TTTGTTGACC ACAACTCCCG AACCACCACT 
TTCATCGATC CCCGACTCCC GCTTCAGAGC AGCAGACCCA CGAGTGCACT GGTGCATCGA 
CAACACCTGA CCCGGCAGCG CAGCCACAGC GCAGGCGAGG TAGGAGAAGA TTCTCGACAT 
GCAGGACCAC CAGTTCTTCC CAGGCCGTCG AGTACGTTCA ATACAGTCAG CAGGCCGCAG 
TACCAGGACA TGGTTCCAGT GGCTTACAAT GACAAGATTG TTGCATTTCT GCGCCAGCCC 
AACATCTTTG AAATTCTGCA GGAACGTCAA CCTGATCTTA CCAGGAATCA CTCACTCAGG 
GAGAAGATCC AATTTATCCG AACTGAAGGG ACTCCAGGAT TGGTGCGTCT TTCAAGTGAT 
GCAGACCTTG TCATGCTGCT GAGTTTATTT GAAGAAGAGA TAATGTCATA TGTGCCTCCA 
CATGCCTTAC TCCATCCCAG CTACTGTCAG TCCCCACGTG GCTCCCCTGT GTCCTCTCCC 
CAGAACTCAC CAGGTACTCA GCGTGCCAAT GCCCGCGCTC CAGCCCCTTA CAAGCGAGAC 
TTTGAAGCCA AACTAAGGAA CTTTTACAGG AAGTTAGAGA CTAAAGGATA TGGACAAGGC 
CCAGGGAAAT TAAAGTTAAT TATCAGAAGA GATCACTTGC TTGAAGATGC TTTTAATCAA 
ATTATGGGCT ACTCCAGAAA AGACCTGCAG AGAAATAAGC TGTACGTCAC ATTCGTTGGA 
GAGGAAGGGC TGGATTACAG TGGACCTTCC AGAGAGTTTT TCTTCCTGGT ATCCAGAGAA 
CTCTTTAACC CATATTATGG CTTATTTGAA TATTCAGCCA ATGACACATA CACAGTACAA 
ATAAGTCCCA TGTCTGCCTT TGTAGACAAT CACCATGAAT GGTTCCGGTT CAGTGGTAGG 
ATCCTTGGTC TTGCACTAAT TCACCAGTAT TTGTTGGATG CCTTCTTCAC ACGGCCATTT 
TATAAGGCTC TTCTCAGAAT TCTCTGTGAC CTGAGTGATC TAGAATACCT TGATGAAGAG 
TTCCATCAGA GCCTGCAGTG GATGAAAGAC AACGACATCC ATGACATCCT GGACCTCACG 
TTCACTGTGA ACGAAGAAGT TTTTGGGCAG ATTACTGAAC GAGAATTAAA ACCAGGGGGT 
GCCAACATCC CAGTCACAGA GAAGAACAAG AAGGAGTACA TTGAGAGGAT GGTGAAATGG 
AGGATTGAGA GGGGTGTCGT GCAGCAGACA GAGAGCTTAG TGCGTGGCTT CTATGAGGTG 
GTGGATGCCA GGCTGGTATC TGTTTTTGAT GCAAGAGAAC TAGAACTGGT CATCGCAGGC 
ACAGCTGAAA TAGACCTAAG TGACTGGAGA AACAACACAG AATACAGAGG AGGATATCAT 
GACAATCACA TTGTAATTCG GTGGTTCTGG GCTGCAGTGG AAAGATTCAA CAATGAACAA 
CGACTGAGGT TGTTACAGTT TGTTACAGGC ACATCCAGCA TTCCCTATGA AGGATTTGCT 
TCTCTCCGAG GCAGTAATGG CCCAAGAAGA TTCTGTGTGG AGAAATGGGG GAAAATCACT 
GCTCTTCCCA GAGCTCACAC TTGTTTTAAC CGTCTGGATC TCCCTCCATA CCCATCCTTC 
TCCATGCTTT ATGAAAAACT CTTGACAGCA GTTGAAGAGA CCAGTACCTT TGGACTTGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006078199.2
CDS	882..5204
Translation	MEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPVWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSIHEDASPEAVGTILGVSSVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHSGHSRSGAATCSERSVGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLASQTKPEDNPVETEEASINEAASFEDKPGNLPELAETSLPSGPVPDESENGPESQPSVDQGSTELCGSQEVDQPTSGADTGTSDTSGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQDSICTAGSLPVVQVPSGEEEGPGSESTTVPDQEEVGEVWQRRGSLEGAAAAAESQPQAEGAARDTPGACEGATAQEEGAIGGCQSNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENSNAIDGAGEEADFHQASADFRRESVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006078137.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006078137.2

ORF Insert Sequence:

ATGGAGCCCG AGATAAAAAT CTGTTTCAAA TACTACCATG GCATCAGTGG AGCCCTGCGA 
GCCACGACCC CTTGCATCAC GGTGAAGAAC CCAGCTGTGA TGATGGGGGC AGAAGGCATG 
GAAGGAGGGG CTTCAGGAAA TCAGCATTCT CGGAAGCTTG TTAGCTTTAC TTTGTCAGAC 
CTTAGGGCAG TTGGGCTAAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG 
TCCATTCAGC CAGGGAAGAA GAGCAGTTTC CCCACCTGTG CCCACCATGG GCAGGAGAGG 
AGGTCTACTA TCATCAGTAA CACCACTAAT CCAGTTTGGC ACCGAGAGAA ATACTCCTTT 
TTTGCACTCC TTACTGATGT CTTAGAGATT GAAATTAAAG ACAAGTTTGC TAAGAGCCGG 
CCTATCATTA AGCGTTTCCT GGGGAAGCTC ACCATTCCAG TCCAGAGGCT ACTGGAGCGG 
CAAGCCATTG GTGACCAGAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC AGCTGACCAT 
GTGAGTGGGT ACCTCCAGTT CAGAGTGGAG GTCACATCTT CTATTCATGA AGATGCCTCT 
CCAGAAGCTG TTGGCACAAT ACTTGGAGTC AGTTCTGTGA ACGGAGACCT AGGAAGCCCT 
TCTGATGAGG AGGACATGCC AGGGGGCCAT CACGACAGCC CAGTTTGTTC CAATGGACCA 
GTTTCTGAGG AAAGTGCTGA TGGGACACCC AAGCATTCTC TCAGGACTAG TTCTACTCTG 
GAAATAGACA CAGAAGAATT AACTTCTACT TCTTCAAGGA CCTCACCTCC CAGAGGACGT 
CAGGATTCAC TCAATGATTA TTTAGATGCT ATTGAACACA GTGGCCATTC TAGGTCAGGT 
GCAGCTACTT GCTCGGAGAG GTCCGTGGGA GCCTCTCCAA AGTTAAGGAG TAGTTTTCCC 
ACTGATACAA GACTCAATGC AATGCTTCAT ATTGACTCAG ATGAAGAAGA CCACGAGTTC 
CAGCAAGACC TGGGCTATCC ATCCTCCTTG GAGGAGGAAG GAGGGTTGAT CATGTTTAGC 
AGGGCATCAA GAACTGATGA TGGGAGCCTG GCATCTCAGA CAAAGCCAGA AGACAACCCA 
GTGGAGACTG AGGAAGCCTC CATTAATGAA GCTGCTTCCT TTGAGGATAA GCCAGGAAAT 
CTTCCAGAGC TTGCAGAGAC CTCTTTACCC TCAGGCCCGG TCCCAGATGA AAGTGAGAAT 
GGCCCAGAAT CTCAACCAAG TGTGGACCAG GGCAGCACTG AATTGTGTGG CTCTCAGGAG 
GTGGATCAGC CCACAAGTGG AGCTGACACG GGCACTTCTG ACACGTCTGG GGGGAGCCGA 
AGAGCTGTCA GTGAGACAGA GTCCCTCGAT CAGGGGTCTG AGCCTTCCCA GGTGTCCTCA 
GAAACAGAGC CCAGCGACCC TGCCAGGACA GAGAGTGTGA GCGAGGCCAG CACCAGGCCC 
GAGGGAGAGA GTGACCTGGA AGGGGCCGAC AGCTCCTGCA ATGAGAGTGT TACCACGCAG 
CTCTCCTCGG TGGAGACACG GTGCTCATCT CTTGAGAGCG CACGGTTTCC TGAAACTCCA 
GCCTTTTCTT CTCAGGAGGA GGAAGATGGA GCCTGTGCAG CAGAGCCCAC CAGCAGTGGC 
CCTGCTGAAG GGTCCCAGGA TTCCATATGC ACTGCTGGTT CCTTACCTGT GGTGCAGGTG 
CCCAGTGGAG AGGAGGAAGG GCCAGGGTCA GAATCAACCA CTGTACCTGA CCAGGAGGAG 
GTGGGGGAGG TCTGGCAGCG GAGGGGGAGC CTGGAGGGAG CTGCTGCTGC TGCTGAGAGC 
CAGCCCCAAG CAGAAGGTGC TGCTCGGGAT ACCCCAGGGG CTTGTGAAGG GGCCACCGCC 
CAGGAGGAGG GCGCGATTGG AGGTTGTCAA TCCAACGGCC ACCAGCCACT GCGATCGCTG 
CCTTCAGTTC GCCAGGATGT TAGCCGGTAC CAGAGGGTGG ACGAGGCTCT CCCACCAAAC 
TGGGAGGCGC GCATTGACAG CCACGGCAGG ATCTTCTACG TGGATCACGT AAACAGAACC 
ACGACGTGGC AGCGGCCCAC AGCTCCCCCT GCCCCGCAGG TGCTGCAGAG GTCCAACTCC 
ATACAGCAGA TGGAGCAGCT GAACCGGCGA TACCAGAGTA TCCGCAGAAC CATGACCAAC 
GAGAGGCCTG AGGAAAATAG CAATGCTATT GATGGGGCCG GGGAGGAAGC CGACTTTCAC 
CAAGCCAGTG CAGATTTCCG ACGGGAGAGC GTCCTGCCCC ACTCTACCTC CAGATCCAGG 
CTCACGTTGC TGTTACAGTC TCCCCCCGTG AAGTTCCTCA TCAGCCCAGA GTTCTTCACC 
GTGCTGCATT CCAACCCTAG TGCCTACCGC ATGTTTACAA ACAACACATG TTTGAAGCAC 
ATGATCACCA AAGTCCGGCG GGACACCCAC CACTTTGAAC GCTACCAGCA TAACCGGGAC 
CTCGTGGGAT TCCTCAACAT GTTTGCAAAC AAACAACTGG AGCTGCCAAG GGGCTGGGAA 
ATGAAACACG ATCATCAGGG CAAGGCATTT TTTGTTGACC ACAACTCCCG AACCACCACT 
TTCATCGATC CCCGACTCCC GCTTCAGAGC AGCAGACCCA CGAGTGCACT GGTGCATCGA 
CAACACCTGA CCCGGCAGCG CAGCCACAGC GCAGGCGAGG TAGGAGAAGA TTCTCGACAT 
GCAGGACCAC CAGTTCTTCC CAGGCCGTCG AGTACGTTCA ATACAGTCAG CAGGCCGCAG 
TACCAGGACA TGGTTCCAGT GGCTTACAAT GACAAGATTG TTGCATTTCT GCGCCAGCCC 
AACATCTTTG AAATTCTGCA GGAACGTCAA CCTGATCTTA CCAGGAATCA CTCACTCAGG 
GAGAAGATCC AATTTATCCG AACTGAAGGG ACTCCAGGAT TGGTGCGTCT TTCAAGTGAT 
GCAGACCTTG TCATGCTGCT GAGTTTATTT GAAGAAGAGA TAATGTCATA TGTGCCTCCA 
CATGCCTTAC TCCATCCCAG CTACTGTCAG TCCCCACGTG GCTCCCCTGT GTCCTCTCCC 
CAGAACTCAC CAGGTACTCA GCGTGCCAAT GCCCGCGCTC CAGCCCCTTA CAAGCGAGAC 
TTTGAAGCCA AACTAAGGAA CTTTTACAGG AAGTTAGAGA CTAAAGGATA TGGACAAGGC 
CCAGGGAAAT TAAAGTTAAT TATCAGAAGA GATCACTTGC TTGAAGATGC TTTTAATCAA 
ATTATGGGCT ACTCCAGAAA AGACCTGCAG AGAAATAAGC TGTACGTCAC ATTCGTTGGA 
GAGGAAGGGC TGGATTACAG TGGACCTTCC AGAGAGTTTT TCTTCCTGGT ATCCAGAGAA 
CTCTTTAACC CATATTATGG CTTATTTGAA TATTCAGCCA ATGACACATA CACAGTACAA 
ATAAGTCCCA TGTCTGCCTT TGTAGACAAT CACCATGAAT GGTTCCGGTT CAGTGGTAGG 
ATCCTTGGTC TTGCACTAAT TCACCAGTAT TTGTTGGATG CCTTCTTCAC ACGGCCATTT 
TATAAGGCTC TTCTCAGAAT TCTCTGTGAC CTGAGTGATC TAGAATACCT TGATGAAGAG 
TTCCATCAGA GCCTGCAGTG GATGAAAGAC AACGACATCC ATGACATCCT GGACCTCACG 
TTCACTGTGA ACGAAGAAGT TTTTGGGCAG ATTACTGAAC GAGAATTAAA ACCAGGGGGT 
GCCAACATCC CAGTCACAGA GAAGAACAAG AAGGAGTACA TTGAGAGGAT GGTGAAATGG 
AGGATTGAGA GGGGTGTCGT GCAGCAGACA GAGAGCTTAG TGCGTGGCTT CTATGAGGTG 
GTGGATGCCA GGCTGGTATC TGTTTTTGAT GCAAGAGAAC TAGAACTGGT CATCGCAGGC 
ACAGCTGAAA TAGACCTAAG TGACTGGAGA AACAACACAG AATACAGAGG AGGATATCAT 
GACAATCACA TTGTAATTCG GTGGTTCTGG GCTGCAGTGG AAAGATTCAA CAATGAACAA 
CGACTGAGGT TGTTACAGTT TGTTACAGGC ACATCCAGCA TTCCCTATGA AGGATTTGCT 
TCTCTCCGAG GCAGTAATGG CCCAAGAAGA TTCTGTGTGG AGAAATGGGG GAAAATCACT 
GCTCTTCCCA GAGCTCACAC TTGTTTTAAC CGTCTGGATC TCCCTCCATA CCCATCCTTC 
TCCATGCTTT ATGAAAAACT CTTGACAGCA GTTGAAGAGA CCAGTACCTT TGGACTTGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_025277891.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133676.1 Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X6, mRNA.

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CloneID	OBu55716
Clone ID Related Accession (Same CDS sequence)	XM_025277891.1
Accession Version	XM_025277891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase HECW2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACAGAGAAT 
ATGACCCTAC AGCGGGCCAA CAGTGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC 
TTGACGGCCA GCATGTATGA GTACACTCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TATATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC 
AAATACTACC ATGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT 
TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT 
AATCCAGTTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG 
ATTGAAATTA AAGACAAGTT TGCTAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG 
CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACAT CTTCTATTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA 
CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAAC ACAGTGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG 
GGAGCCTCTC CAAAGTTAAG GAGTAGTTTT CCCACTGATA CAAGACTCAA TGCAATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC 
TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC 
CTGGCATCTC AGACAAAGCC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA 
CCCTCAGGCC CGGTCCCAGA TGAAAGTGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC 
ACGGGCACTT CTGACACGTC TGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTC 
GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGCGA CCCTGCCAGG 
ACAGAGAGTG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA 
TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGCAGAAGG TGCTGCTCGG 
GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AGGGCGCGAT TGGAGGTTGT 
CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA CGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG 
CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT 
ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG 
AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG 
AGCAGCAGAC CCACGAGTGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC 
AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG 
TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA 
GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTACTGT 
CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG 
CAGAGAAATA AGCTGTACGT CACATTCGTT GGAGAGGAAG GGCTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCACT AATTCACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCA TTTTATAAGG CTCTTCTCAG AATATTTCAA 
GCTCTGTCCC CGCTACCTAC TGGCTGTGTG ACATCAGGCA ACTTATATAA CATTCCTGAG 
CTGAGTTTCT CATCAGTAAA ATTGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133676.1
CDS	150..4259
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMTENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPVWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSIHEDASPEAVGTILGVSSVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHSGHSRSGAATCSERSVGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLASQTKPEDNPVETEEASINEAASFEDKPGNLPELAETSLPSGPVPDESENGPESQPSVDQGSTELCGSQEVDQPTSGADTGTSDTSGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQDSICTAGSLPVVQVPSGEEEGPGSESTTVPDQEEVGEVWQRRGSLEGAAAAAESQPQAEGAARDTPGACEGATAQEEGAIGGCQSNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENSNAIDGAGEEADFHQASADFRRESVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRIFQALSPLPTGCVTSGNLYNIPELSFSSVKLR

Target ORF information:

RefSeq Version	XM_025277891.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025277891.1

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACAGAGAAT 
ATGACCCTAC AGCGGGCCAA CAGTGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC 
TTGACGGCCA GCATGTATGA GTACACTCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TATATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC 
AAATACTACC ATGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT 
TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT 
AATCCAGTTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG 
ATTGAAATTA AAGACAAGTT TGCTAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG 
CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACAT CTTCTATTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA 
CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAAC ACAGTGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG 
GGAGCCTCTC CAAAGTTAAG GAGTAGTTTT CCCACTGATA CAAGACTCAA TGCAATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC 
TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC 
CTGGCATCTC AGACAAAGCC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA 
CCCTCAGGCC CGGTCCCAGA TGAAAGTGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC 
ACGGGCACTT CTGACACGTC TGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTC 
GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGCGA CCCTGCCAGG 
ACAGAGAGTG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA 
TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGCAGAAGG TGCTGCTCGG 
GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AGGGCGCGAT TGGAGGTTGT 
CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA CGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG 
CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT 
ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG 
AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG 
AGCAGCAGAC CCACGAGTGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC 
AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG 
TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA 
GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTACTGT 
CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG 
CAGAGAAATA AGCTGTACGT CACATTCGTT GGAGAGGAAG GGCTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCACT AATTCACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCA TTTTATAAGG CTCTTCTCAG AATATTTCAA 
GCTCTGTCCC CGCTACCTAC TGGCTGTGTG ACATCAGGCA ACTTATATAA CATTCCTGAG 
CTGAGTTTCT CATCAGTAAA ATTGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_006078133.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006078195.1 Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

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CloneID	OBu31385
Clone ID Related Accession (Same CDS sequence)	XM_006078133.1 , XM_006078134.1 , XM_006078136.1 , XM_006078135.1
Accession Version	XM_006078133.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785813.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACAGAGAAT 
ATGACCCTAC AGCGGGCCAA CAGTGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC 
TTGACGGCCA GCATGTATGA GTACACTCTG GGGCAAGCCC AGAACCTCAT CATCTTCTGG 
GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TATATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT 
TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT 
AATCCAGTTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG 
ATTGAAATTA AAGACAAGTT TGCTAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG 
CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACAT CTTCTATTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA 
CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAAC ACAGTGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG 
GGAGCCTCTC CAAAGTTAAG GAGTAGTTTT CCCACTGATA CAAGACTCAA TGCAATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC 
TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC 
CTGGCATCTC AGACAAAGCC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA 
CCCTCAGGCC CGGTCCCAGA TGAAAGTGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC 
ACGGGCACTT CTGACACGTC TGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTC 
GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGCGA CCCTGCCAGG 
ACAGAGAGCG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CGCCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA 
TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGCAGAGGG TGCTGCTCGG 
GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AGGGCGCGAT TGGAGGTTGT 
CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA CGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG 
CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT 
ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG 
AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG 
AGCAGCAGAC CCACGAGTGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC 
AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG 
TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA 
GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTACTGT 
CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG 
CAGAGAAATA AGCTGTACGT CACATTCGTT GGAGAGGAAG GGCTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCACT AATTCACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCA TTTTATAAGG CTCTTCTCAG AATTCTCTGT 
GACCTGAGTG ATCTAGAATA CCTTGATGAA GAGTTCCATC AGAGCCTGCA GTGGATGAAA 
GACAACGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAACGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAACA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATTGAGAG GATGGTGAAA TGGAGGATTG AGAGGGGTGT CGTGCAGCAG 
ACAGAGAGCT TAGTGCGTGG CTTCTATGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT GGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATACAG AGGAGGATAT CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTGA GGTTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGCAGTAA TGGCCCAAGA 
AGATTCTGTG TGGAGAAATG GGGGAAAATC ACTGCTCTTC CCAGAGCTCA CACTTGTTTT 
AACCGTCTGG ATCTCCCTCC ATACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006078195.1
CDS	146..4861
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMTENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPVWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSIHEDASPEAVGTILGVSSVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHSGHSRSGAATCSERSVGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLASQTKPEDNPVETEEASINEAASFEDKPGNLPELAETSLPSGPVPDESENGPESQPSVDQGSTELCGSQEVDQPTSGADTGTSDTSGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPASSGPAEGSQDSICTAGSLPVVQVPSGEEEGPGSESTTVPDQEEVGEVWQRRGSLEGAAAAAESQPQAEGAARDTPGACEGATAQEEGAIGGCQSNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENSNAIDGAGEEADFHQASADFRRESVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006078133.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006078133.1

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACAGAGAAT 
ATGACCCTAC AGCGGGCCAA CAGTGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC 
TTGACGGCCA GCATGTATGA GTACACTCTG GGGCAAGCCC AGAACCTCAT CATCTTCTGG 
GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TATATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT 
TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT 
AATCCAGTTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG 
ATTGAAATTA AAGACAAGTT TGCTAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG 
CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACAT CTTCTATTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA 
CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAAC ACAGTGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG 
GGAGCCTCTC CAAAGTTAAG GAGTAGTTTT CCCACTGATA CAAGACTCAA TGCAATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC 
TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC 
CTGGCATCTC AGACAAAGCC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA 
CCCTCAGGCC CGGTCCCAGA TGAAAGTGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC 
ACGGGCACTT CTGACACGTC TGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTC 
GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGCGA CCCTGCCAGG 
ACAGAGAGCG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CGCCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA 
TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGCAGAGGG TGCTGCTCGG 
GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AGGGCGCGAT TGGAGGTTGT 
CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA CGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG 
CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT 
ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG 
AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG 
AGCAGCAGAC CCACGAGTGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC 
AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG 
TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA 
GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTACTGT 
CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG 
CAGAGAAATA AGCTGTACGT CACATTCGTT GGAGAGGAAG GGCTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCACT AATTCACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCA TTTTATAAGG CTCTTCTCAG AATTCTCTGT 
GACCTGAGTG ATCTAGAATA CCTTGATGAA GAGTTCCATC AGAGCCTGCA GTGGATGAAA 
GACAACGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAACGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAACA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATTGAGAG GATGGTGAAA TGGAGGATTG AGAGGGGTGT CGTGCAGCAG 
ACAGAGAGCT TAGTGCGTGG CTTCTATGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT GGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATACAG AGGAGGATAT CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTGA GGTTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGCAGTAA TGGCCCAAGA 
AGATTCTGTG TGGAGAAATG GGGGAAAATC ACTGCTCTTC CCAGAGCTCA CACTTGTTTT 
AACCGTCTGG ATCTCCCTCC ATACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_006078134.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006078196.1 Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu31385
Clone ID Related Accession (Same CDS sequence)	XM_006078133.1 , XM_006078134.1 , XM_006078136.1 , XM_006078135.1
Accession Version	XM_006078134.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase HECW2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785813.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACAGAGAAT 
ATGACCCTAC AGCGGGCCAA CAGTGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC 
TTGACGGCCA GCATGTATGA GTACACTCTG GGGCAAGCCC AGAACCTCAT CATCTTCTGG 
GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TATATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT 
TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT 
AATCCAGTTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG 
ATTGAAATTA AAGACAAGTT TGCTAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG 
CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACAT CTTCTATTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA 
CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAAC ACAGTGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG 
GGAGCCTCTC CAAAGTTAAG GAGTAGTTTT CCCACTGATA CAAGACTCAA TGCAATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC 
TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC 
CTGGCATCTC AGACAAAGCC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA 
CCCTCAGGCC CGGTCCCAGA TGAAAGTGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC 
ACGGGCACTT CTGACACGTC TGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTC 
GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGCGA CCCTGCCAGG 
ACAGAGAGCG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CGCCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA 
TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGCAGAGGG TGCTGCTCGG 
GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AGGGCGCGAT TGGAGGTTGT 
CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA CGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG 
CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT 
ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG 
AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG 
AGCAGCAGAC CCACGAGTGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC 
AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG 
TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA 
GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTACTGT 
CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG 
CAGAGAAATA AGCTGTACGT CACATTCGTT GGAGAGGAAG GGCTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCACT AATTCACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCA TTTTATAAGG CTCTTCTCAG AATTCTCTGT 
GACCTGAGTG ATCTAGAATA CCTTGATGAA GAGTTCCATC AGAGCCTGCA GTGGATGAAA 
GACAACGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAACGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAACA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATTGAGAG GATGGTGAAA TGGAGGATTG AGAGGGGTGT CGTGCAGCAG 
ACAGAGAGCT TAGTGCGTGG CTTCTATGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT GGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATACAG AGGAGGATAT CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTGA GGTTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGCAGTAA TGGCCCAAGA 
AGATTCTGTG TGGAGAAATG GGGGAAAATC ACTGCTCTTC CCAGAGCTCA CACTTGTTTT 
AACCGTCTGG ATCTCCCTCC ATACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006078196.1
CDS	80..4795
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMTENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPVWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSIHEDASPEAVGTILGVSSVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHSGHSRSGAATCSERSVGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLASQTKPEDNPVETEEASINEAASFEDKPGNLPELAETSLPSGPVPDESENGPESQPSVDQGSTELCGSQEVDQPTSGADTGTSDTSGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPASSGPAEGSQDSICTAGSLPVVQVPSGEEEGPGSESTTVPDQEEVGEVWQRRGSLEGAAAAAESQPQAEGAARDTPGACEGATAQEEGAIGGCQSNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENSNAIDGAGEEADFHQASADFRRESVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006078134.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006078134.1

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACAGAGAAT 
ATGACCCTAC AGCGGGCCAA CAGTGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC 
TTGACGGCCA GCATGTATGA GTACACTCTG GGGCAAGCCC AGAACCTCAT CATCTTCTGG 
GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TATATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT 
TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT 
AATCCAGTTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG 
ATTGAAATTA AAGACAAGTT TGCTAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG 
CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACAT CTTCTATTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA 
CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAAC ACAGTGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG 
GGAGCCTCTC CAAAGTTAAG GAGTAGTTTT CCCACTGATA CAAGACTCAA TGCAATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC 
TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC 
CTGGCATCTC AGACAAAGCC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA 
CCCTCAGGCC CGGTCCCAGA TGAAAGTGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC 
ACGGGCACTT CTGACACGTC TGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTC 
GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGCGA CCCTGCCAGG 
ACAGAGAGCG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CGCCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA 
TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGCAGAGGG TGCTGCTCGG 
GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AGGGCGCGAT TGGAGGTTGT 
CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA CGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG 
CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT 
ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG 
AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG 
AGCAGCAGAC CCACGAGTGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC 
AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG 
TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA 
GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTACTGT 
CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG 
CAGAGAAATA AGCTGTACGT CACATTCGTT GGAGAGGAAG GGCTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCACT AATTCACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCA TTTTATAAGG CTCTTCTCAG AATTCTCTGT 
GACCTGAGTG ATCTAGAATA CCTTGATGAA GAGTTCCATC AGAGCCTGCA GTGGATGAAA 
GACAACGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAACGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAACA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATTGAGAG GATGGTGAAA TGGAGGATTG AGAGGGGTGT CGTGCAGCAG 
ACAGAGAGCT TAGTGCGTGG CTTCTATGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT GGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATACAG AGGAGGATAT CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTGA GGTTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGCAGTAA TGGCCCAAGA 
AGATTCTGTG TGGAGAAATG GGGGAAAATC ACTGCTCTTC CCAGAGCTCA CACTTGTTTT 
AACCGTCTGG ATCTCCCTCC ATACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_006078136.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006078198.1 Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu31385
Clone ID Related Accession (Same CDS sequence)	XM_006078133.1 , XM_006078134.1 , XM_006078136.1 , XM_006078135.1
Accession Version	XM_006078136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase HECW2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785813.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACAGAGAAT 
ATGACCCTAC AGCGGGCCAA CAGTGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC 
TTGACGGCCA GCATGTATGA GTACACTCTG GGGCAAGCCC AGAACCTCAT CATCTTCTGG 
GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TATATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT 
TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT 
AATCCAGTTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG 
ATTGAAATTA AAGACAAGTT TGCTAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG 
CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACAT CTTCTATTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA 
CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAAC ACAGTGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG 
GGAGCCTCTC CAAAGTTAAG GAGTAGTTTT CCCACTGATA CAAGACTCAA TGCAATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC 
TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC 
CTGGCATCTC AGACAAAGCC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA 
CCCTCAGGCC CGGTCCCAGA TGAAAGTGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC 
ACGGGCACTT CTGACACGTC TGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTC 
GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGCGA CCCTGCCAGG 
ACAGAGAGCG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CGCCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA 
TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGCAGAGGG TGCTGCTCGG 
GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AGGGCGCGAT TGGAGGTTGT 
CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA CGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG 
CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT 
ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG 
AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG 
AGCAGCAGAC CCACGAGTGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC 
AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG 
TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA 
GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTACTGT 
CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG 
CAGAGAAATA AGCTGTACGT CACATTCGTT GGAGAGGAAG GGCTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCACT AATTCACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCA TTTTATAAGG CTCTTCTCAG AATTCTCTGT 
GACCTGAGTG ATCTAGAATA CCTTGATGAA GAGTTCCATC AGAGCCTGCA GTGGATGAAA 
GACAACGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAACGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAACA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATTGAGAG GATGGTGAAA TGGAGGATTG AGAGGGGTGT CGTGCAGCAG 
ACAGAGAGCT TAGTGCGTGG CTTCTATGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT GGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATACAG AGGAGGATAT CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTGA GGTTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGCAGTAA TGGCCCAAGA 
AGATTCTGTG TGGAGAAATG GGGGAAAATC ACTGCTCTTC CCAGAGCTCA CACTTGTTTT 
AACCGTCTGG ATCTCCCTCC ATACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006078198.1
CDS	51..4766
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMTENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPVWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSIHEDASPEAVGTILGVSSVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHSGHSRSGAATCSERSVGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLASQTKPEDNPVETEEASINEAASFEDKPGNLPELAETSLPSGPVPDESENGPESQPSVDQGSTELCGSQEVDQPTSGADTGTSDTSGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPASSGPAEGSQDSICTAGSLPVVQVPSGEEEGPGSESTTVPDQEEVGEVWQRRGSLEGAAAAAESQPQAEGAARDTPGACEGATAQEEGAIGGCQSNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENSNAIDGAGEEADFHQASADFRRESVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006078136.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006078136.1

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACAGAGAAT 
ATGACCCTAC AGCGGGCCAA CAGTGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC 
TTGACGGCCA GCATGTATGA GTACACTCTG GGGCAAGCCC AGAACCTCAT CATCTTCTGG 
GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TATATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT 
TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT 
AATCCAGTTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG 
ATTGAAATTA AAGACAAGTT TGCTAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG 
CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACAT CTTCTATTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA 
CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAAC ACAGTGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG 
GGAGCCTCTC CAAAGTTAAG GAGTAGTTTT CCCACTGATA CAAGACTCAA TGCAATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC 
TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC 
CTGGCATCTC AGACAAAGCC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA 
CCCTCAGGCC CGGTCCCAGA TGAAAGTGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC 
ACGGGCACTT CTGACACGTC TGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTC 
GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGCGA CCCTGCCAGG 
ACAGAGAGCG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CGCCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA 
TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGCAGAGGG TGCTGCTCGG 
GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AGGGCGCGAT TGGAGGTTGT 
CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA CGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG 
CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT 
ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG 
AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG 
AGCAGCAGAC CCACGAGTGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC 
AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG 
TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA 
GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTACTGT 
CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG 
CAGAGAAATA AGCTGTACGT CACATTCGTT GGAGAGGAAG GGCTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCACT AATTCACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCA TTTTATAAGG CTCTTCTCAG AATTCTCTGT 
GACCTGAGTG ATCTAGAATA CCTTGATGAA GAGTTCCATC AGAGCCTGCA GTGGATGAAA 
GACAACGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAACGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAACA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATTGAGAG GATGGTGAAA TGGAGGATTG AGAGGGGTGT CGTGCAGCAG 
ACAGAGAGCT TAGTGCGTGG CTTCTATGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT GGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATACAG AGGAGGATAT CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTGA GGTTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGCAGTAA TGGCCCAAGA 
AGATTCTGTG TGGAGAAATG GGGGAAAATC ACTGCTCTTC CCAGAGCTCA CACTTGTTTT 
AACCGTCTGG ATCTCCCTCC ATACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_006078135.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006078197.1 Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.

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CloneID	OBu31385
Clone ID Related Accession (Same CDS sequence)	XM_006078133.1 , XM_006078134.1 , XM_006078136.1 , XM_006078135.1
Accession Version	XM_006078135.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase HECW2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785813.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACAGAGAAT 
ATGACCCTAC AGCGGGCCAA CAGTGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC 
TTGACGGCCA GCATGTATGA GTACACTCTG GGGCAAGCCC AGAACCTCAT CATCTTCTGG 
GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TATATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT 
TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT 
AATCCAGTTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG 
ATTGAAATTA AAGACAAGTT TGCTAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG 
CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACAT CTTCTATTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA 
CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAAC ACAGTGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG 
GGAGCCTCTC CAAAGTTAAG GAGTAGTTTT CCCACTGATA CAAGACTCAA TGCAATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC 
TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC 
CTGGCATCTC AGACAAAGCC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA 
CCCTCAGGCC CGGTCCCAGA TGAAAGTGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC 
ACGGGCACTT CTGACACGTC TGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTC 
GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGCGA CCCTGCCAGG 
ACAGAGAGCG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CGCCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA 
TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGCAGAGGG TGCTGCTCGG 
GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AGGGCGCGAT TGGAGGTTGT 
CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA CGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG 
CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT 
ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG 
AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG 
AGCAGCAGAC CCACGAGTGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC 
AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG 
TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA 
GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTACTGT 
CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG 
CAGAGAAATA AGCTGTACGT CACATTCGTT GGAGAGGAAG GGCTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCACT AATTCACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCA TTTTATAAGG CTCTTCTCAG AATTCTCTGT 
GACCTGAGTG ATCTAGAATA CCTTGATGAA GAGTTCCATC AGAGCCTGCA GTGGATGAAA 
GACAACGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAACGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAACA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATTGAGAG GATGGTGAAA TGGAGGATTG AGAGGGGTGT CGTGCAGCAG 
ACAGAGAGCT TAGTGCGTGG CTTCTATGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT GGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATACAG AGGAGGATAT CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTGA GGTTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGCAGTAA TGGCCCAAGA 
AGATTCTGTG TGGAGAAATG GGGGAAAATC ACTGCTCTTC CCAGAGCTCA CACTTGTTTT 
AACCGTCTGG ATCTCCCTCC ATACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006078197.1
CDS	52..4767
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMTENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPVWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSIHEDASPEAVGTILGVSSVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHSGHSRSGAATCSERSVGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLASQTKPEDNPVETEEASINEAASFEDKPGNLPELAETSLPSGPVPDESENGPESQPSVDQGSTELCGSQEVDQPTSGADTGTSDTSGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPASSGPAEGSQDSICTAGSLPVVQVPSGEEEGPGSESTTVPDQEEVGEVWQRRGSLEGAAAAAESQPQAEGAARDTPGACEGATAQEEGAIGGCQSNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENSNAIDGAGEEADFHQASADFRRESVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006078135.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006078135.1

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACAGAGAAT 
ATGACCCTAC AGCGGGCCAA CAGTGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC 
TTGACGGCCA GCATGTATGA GTACACTCTG GGGCAAGCCC AGAACCTCAT CATCTTCTGG 
GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TATATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC 
AAATACTACC ACGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT 
TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT 
AATCCAGTTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG 
ATTGAAATTA AAGACAAGTT TGCTAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG 
CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACAT CTTCTATTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA 
CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAAC ACAGTGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG 
GGAGCCTCTC CAAAGTTAAG GAGTAGTTTT CCCACTGATA CAAGACTCAA TGCAATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC 
TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC 
CTGGCATCTC AGACAAAGCC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA 
CCCTCAGGCC CGGTCCCAGA TGAAAGTGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC 
ACGGGCACTT CTGACACGTC TGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTC 
GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGCGA CCCTGCCAGG 
ACAGAGAGCG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CGCCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA 
TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGCAGAGGG TGCTGCTCGG 
GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AGGGCGCGAT TGGAGGTTGT 
CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA CGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG 
CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT 
ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG 
AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG 
AGCAGCAGAC CCACGAGTGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC 
AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG 
TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA 
GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTACTGT 
CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG 
CAGAGAAATA AGCTGTACGT CACATTCGTT GGAGAGGAAG GGCTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCACT AATTCACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCA TTTTATAAGG CTCTTCTCAG AATTCTCTGT 
GACCTGAGTG ATCTAGAATA CCTTGATGAA GAGTTCCATC AGAGCCTGCA GTGGATGAAA 
GACAACGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAACGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAACA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATTGAGAG GATGGTGAAA TGGAGGATTG AGAGGGGTGT CGTGCAGCAG 
ACAGAGAGCT TAGTGCGTGG CTTCTATGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT GGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATACAG AGGAGGATAT CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTGA GGTTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGCAGTAA TGGCCCAAGA 
AGATTCTGTG TGGAGAAATG GGGGAAAATC ACTGCTCTTC CCAGAGCTCA CACTTGTTTT 
AACCGTCTGG ATCTCCCTCC ATACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_006078135.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006078197.2 Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.

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CloneID	OBu55713
Clone ID Related Accession (Same CDS sequence)	XM_006078135.2 , XM_006078136.2 , XM_025277874.1
Accession Version	XM_006078135.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006078135.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACAGAGAAT 
ATGACCCTAC AGCGGGCCAA CAGTGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC 
TTGACGGCCA GCATGTATGA GTACACTCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TATATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC 
AAATACTACC ATGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT 
TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT 
AATCCAGTTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG 
ATTGAAATTA AAGACAAGTT TGCTAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG 
CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACAT CTTCTATTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA 
CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAAC ACAGTGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG 
GGAGCCTCTC CAAAGTTAAG GAGTAGTTTT CCCACTGATA CAAGACTCAA TGCAATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC 
TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC 
CTGGCATCTC AGACAAAGCC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA 
CCCTCAGGCC CGGTCCCAGA TGAAAGTGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC 
ACGGGCACTT CTGACACGTC TGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTC 
GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGCGA CCCTGCCAGG 
ACAGAGAGTG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA 
TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGCAGAAGG TGCTGCTCGG 
GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AGGGCGCGAT TGGAGGTTGT 
CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA CGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG 
CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT 
ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG 
AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG 
AGCAGCAGAC CCACGAGTGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC 
AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG 
TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA 
GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTACTGT 
CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG 
CAGAGAAATA AGCTGTACGT CACATTCGTT GGAGAGGAAG GGCTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCACT AATTCACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCA TTTTATAAGG CTCTTCTCAG AATTCTCTGT 
GACCTGAGTG ATCTAGAATA CCTTGATGAA GAGTTCCATC AGAGCCTGCA GTGGATGAAA 
GACAACGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAACGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAACA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATTGAGAG GATGGTGAAA TGGAGGATTG AGAGGGGTGT CGTGCAGCAG 
ACAGAGAGCT TAGTGCGTGG CTTCTATGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT GGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATACAG AGGAGGATAT CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTGA GGTTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGCAGTAA TGGCCCAAGA 
AGATTCTGTG TGGAGAAATG GGGGAAAATC ACTGCTCTTC CCAGAGCTCA CACTTGTTTT 
AACCGTCTGG ATCTCCCTCC ATACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006078197.2
CDS	133..4848
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMTENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPVWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSIHEDASPEAVGTILGVSSVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHSGHSRSGAATCSERSVGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLASQTKPEDNPVETEEASINEAASFEDKPGNLPELAETSLPSGPVPDESENGPESQPSVDQGSTELCGSQEVDQPTSGADTGTSDTSGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQDSICTAGSLPVVQVPSGEEEGPGSESTTVPDQEEVGEVWQRRGSLEGAAAAAESQPQAEGAARDTPGACEGATAQEEGAIGGCQSNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENSNAIDGAGEEADFHQASADFRRESVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006078135.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006078135.2

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACAGAGAAT 
ATGACCCTAC AGCGGGCCAA CAGTGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC 
TTGACGGCCA GCATGTATGA GTACACTCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TATATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC 
AAATACTACC ATGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT 
TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT 
AATCCAGTTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG 
ATTGAAATTA AAGACAAGTT TGCTAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG 
CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACAT CTTCTATTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA 
CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAAC ACAGTGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG 
GGAGCCTCTC CAAAGTTAAG GAGTAGTTTT CCCACTGATA CAAGACTCAA TGCAATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC 
TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC 
CTGGCATCTC AGACAAAGCC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA 
CCCTCAGGCC CGGTCCCAGA TGAAAGTGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC 
ACGGGCACTT CTGACACGTC TGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTC 
GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGCGA CCCTGCCAGG 
ACAGAGAGTG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA 
TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGCAGAAGG TGCTGCTCGG 
GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AGGGCGCGAT TGGAGGTTGT 
CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA CGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG 
CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT 
ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG 
AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG 
AGCAGCAGAC CCACGAGTGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC 
AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG 
TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA 
GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTACTGT 
CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG 
CAGAGAAATA AGCTGTACGT CACATTCGTT GGAGAGGAAG GGCTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCACT AATTCACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCA TTTTATAAGG CTCTTCTCAG AATTCTCTGT 
GACCTGAGTG ATCTAGAATA CCTTGATGAA GAGTTCCATC AGAGCCTGCA GTGGATGAAA 
GACAACGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAACGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAACA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATTGAGAG GATGGTGAAA TGGAGGATTG AGAGGGGTGT CGTGCAGCAG 
ACAGAGAGCT TAGTGCGTGG CTTCTATGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT GGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATACAG AGGAGGATAT CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTGA GGTTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGCAGTAA TGGCCCAAGA 
AGATTCTGTG TGGAGAAATG GGGGAAAATC ACTGCTCTTC CCAGAGCTCA CACTTGTTTT 
AACCGTCTGG ATCTCCCTCC ATACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_006078136.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006078198.2 Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

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CloneID	OBu55713
Clone ID Related Accession (Same CDS sequence)	XM_006078135.2 , XM_006078136.2 , XM_025277874.1
Accession Version	XM_006078136.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006078136.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACAGAGAAT 
ATGACCCTAC AGCGGGCCAA CAGTGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC 
TTGACGGCCA GCATGTATGA GTACACTCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TATATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC 
AAATACTACC ATGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT 
TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT 
AATCCAGTTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG 
ATTGAAATTA AAGACAAGTT TGCTAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG 
CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACAT CTTCTATTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA 
CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAAC ACAGTGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG 
GGAGCCTCTC CAAAGTTAAG GAGTAGTTTT CCCACTGATA CAAGACTCAA TGCAATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC 
TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC 
CTGGCATCTC AGACAAAGCC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA 
CCCTCAGGCC CGGTCCCAGA TGAAAGTGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC 
ACGGGCACTT CTGACACGTC TGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTC 
GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGCGA CCCTGCCAGG 
ACAGAGAGTG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA 
TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGCAGAAGG TGCTGCTCGG 
GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AGGGCGCGAT TGGAGGTTGT 
CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA CGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG 
CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT 
ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG 
AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG 
AGCAGCAGAC CCACGAGTGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC 
AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG 
TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA 
GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTACTGT 
CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG 
CAGAGAAATA AGCTGTACGT CACATTCGTT GGAGAGGAAG GGCTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCACT AATTCACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCA TTTTATAAGG CTCTTCTCAG AATTCTCTGT 
GACCTGAGTG ATCTAGAATA CCTTGATGAA GAGTTCCATC AGAGCCTGCA GTGGATGAAA 
GACAACGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAACGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAACA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATTGAGAG GATGGTGAAA TGGAGGATTG AGAGGGGTGT CGTGCAGCAG 
ACAGAGAGCT TAGTGCGTGG CTTCTATGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT GGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATACAG AGGAGGATAT CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTGA GGTTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGCAGTAA TGGCCCAAGA 
AGATTCTGTG TGGAGAAATG GGGGAAAATC ACTGCTCTTC CCAGAGCTCA CACTTGTTTT 
AACCGTCTGG ATCTCCCTCC ATACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006078198.2
CDS	174..4889
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMTENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPVWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSIHEDASPEAVGTILGVSSVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHSGHSRSGAATCSERSVGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLASQTKPEDNPVETEEASINEAASFEDKPGNLPELAETSLPSGPVPDESENGPESQPSVDQGSTELCGSQEVDQPTSGADTGTSDTSGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQDSICTAGSLPVVQVPSGEEEGPGSESTTVPDQEEVGEVWQRRGSLEGAAAAAESQPQAEGAARDTPGACEGATAQEEGAIGGCQSNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENSNAIDGAGEEADFHQASADFRRESVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006078136.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006078136.2

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACAGAGAAT 
ATGACCCTAC AGCGGGCCAA CAGTGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC 
TTGACGGCCA GCATGTATGA GTACACTCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TATATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC 
AAATACTACC ATGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT 
TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT 
AATCCAGTTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG 
ATTGAAATTA AAGACAAGTT TGCTAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG 
CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACAT CTTCTATTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA 
CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAAC ACAGTGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG 
GGAGCCTCTC CAAAGTTAAG GAGTAGTTTT CCCACTGATA CAAGACTCAA TGCAATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC 
TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC 
CTGGCATCTC AGACAAAGCC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA 
CCCTCAGGCC CGGTCCCAGA TGAAAGTGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC 
ACGGGCACTT CTGACACGTC TGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTC 
GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGCGA CCCTGCCAGG 
ACAGAGAGTG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA 
TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGCAGAAGG TGCTGCTCGG 
GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AGGGCGCGAT TGGAGGTTGT 
CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA CGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG 
CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT 
ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG 
AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG 
AGCAGCAGAC CCACGAGTGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC 
AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG 
TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA 
GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTACTGT 
CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG 
CAGAGAAATA AGCTGTACGT CACATTCGTT GGAGAGGAAG GGCTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCACT AATTCACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCA TTTTATAAGG CTCTTCTCAG AATTCTCTGT 
GACCTGAGTG ATCTAGAATA CCTTGATGAA GAGTTCCATC AGAGCCTGCA GTGGATGAAA 
GACAACGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAACGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAACA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATTGAGAG GATGGTGAAA TGGAGGATTG AGAGGGGTGT CGTGCAGCAG 
ACAGAGAGCT TAGTGCGTGG CTTCTATGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT GGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATACAG AGGAGGATAT CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTGA GGTTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGCAGTAA TGGCCCAAGA 
AGATTCTGTG TGGAGAAATG GGGGAAAATC ACTGCTCTTC CCAGAGCTCA CACTTGTTTT 
AACCGTCTGG ATCTCCCTCC ATACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_025277874.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133659.1 Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

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CloneID	OBu55713
Clone ID Related Accession (Same CDS sequence)	XM_006078135.2 , XM_006078136.2 , XM_025277874.1
Accession Version	XM_025277874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACAGAGAAT 
ATGACCCTAC AGCGGGCCAA CAGTGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC 
TTGACGGCCA GCATGTATGA GTACACTCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TATATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC 
AAATACTACC ATGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT 
TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT 
AATCCAGTTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG 
ATTGAAATTA AAGACAAGTT TGCTAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG 
CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACAT CTTCTATTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA 
CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAAC ACAGTGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG 
GGAGCCTCTC CAAAGTTAAG GAGTAGTTTT CCCACTGATA CAAGACTCAA TGCAATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC 
TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC 
CTGGCATCTC AGACAAAGCC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA 
CCCTCAGGCC CGGTCCCAGA TGAAAGTGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC 
ACGGGCACTT CTGACACGTC TGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTC 
GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGCGA CCCTGCCAGG 
ACAGAGAGTG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA 
TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGCAGAAGG TGCTGCTCGG 
GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AGGGCGCGAT TGGAGGTTGT 
CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA CGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG 
CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT 
ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG 
AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG 
AGCAGCAGAC CCACGAGTGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC 
AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG 
TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA 
GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTACTGT 
CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG 
CAGAGAAATA AGCTGTACGT CACATTCGTT GGAGAGGAAG GGCTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCACT AATTCACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCA TTTTATAAGG CTCTTCTCAG AATTCTCTGT 
GACCTGAGTG ATCTAGAATA CCTTGATGAA GAGTTCCATC AGAGCCTGCA GTGGATGAAA 
GACAACGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAACGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAACA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATTGAGAG GATGGTGAAA TGGAGGATTG AGAGGGGTGT CGTGCAGCAG 
ACAGAGAGCT TAGTGCGTGG CTTCTATGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT GGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATACAG AGGAGGATAT CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTGA GGTTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGCAGTAA TGGCCCAAGA 
AGATTCTGTG TGGAGAAATG GGGGAAAATC ACTGCTCTTC CCAGAGCTCA CACTTGTTTT 
AACCGTCTGG ATCTCCCTCC ATACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133659.1
CDS	150..4865
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMTENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPVWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSIHEDASPEAVGTILGVSSVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHSGHSRSGAATCSERSVGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLASQTKPEDNPVETEEASINEAASFEDKPGNLPELAETSLPSGPVPDESENGPESQPSVDQGSTELCGSQEVDQPTSGADTGTSDTSGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQDSICTAGSLPVVQVPSGEEEGPGSESTTVPDQEEVGEVWQRRGSLEGAAAAAESQPQAEGAARDTPGACEGATAQEEGAIGGCQSNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENSNAIDGAGEEADFHQASADFRRESVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_025277874.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025277874.1

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACAGAGAAT 
ATGACCCTAC AGCGGGCCAA CAGTGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC 
TTGACGGCCA GCATGTATGA GTACACTCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TATATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC 
AAATACTACC ATGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT 
TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT 
AATCCAGTTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG 
ATTGAAATTA AAGACAAGTT TGCTAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG 
CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACAT CTTCTATTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA 
CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAAC ACAGTGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG 
GGAGCCTCTC CAAAGTTAAG GAGTAGTTTT CCCACTGATA CAAGACTCAA TGCAATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC 
TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC 
CTGGCATCTC AGACAAAGCC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA 
CCCTCAGGCC CGGTCCCAGA TGAAAGTGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC 
ACGGGCACTT CTGACACGTC TGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTC 
GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGCGA CCCTGCCAGG 
ACAGAGAGTG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA 
TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGCAGAAGG TGCTGCTCGG 
GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AGGGCGCGAT TGGAGGTTGT 
CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA CGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG 
CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT 
ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG 
AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG 
AGCAGCAGAC CCACGAGTGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC 
AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG 
TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA 
GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTACTGT 
CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG 
CAGAGAAATA AGCTGTACGT CACATTCGTT GGAGAGGAAG GGCTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCACT AATTCACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCA TTTTATAAGG CTCTTCTCAG AATTCTCTGT 
GACCTGAGTG ATCTAGAATA CCTTGATGAA GAGTTCCATC AGAGCCTGCA GTGGATGAAA 
GACAACGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAACGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAACA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATTGAGAG GATGGTGAAA TGGAGGATTG AGAGGGGTGT CGTGCAGCAG 
ACAGAGAGCT TAGTGCGTGG CTTCTATGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT GGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATACAG AGGAGGATAT CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTGA GGTTGTTACA GTTTGTTACA 
GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGCAGTAA TGGCCCAAGA 
AGATTCTGTG TGGAGAAATG GGGGAAAATC ACTGCTCTTC CCAGAGCTCA CACTTGTTTT 
AACCGTCTGG ATCTCCCTCC ATACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA 
GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_025277884.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133669.1 Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.

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CloneID	OBu55714
Clone ID Related Accession (Same CDS sequence)	XM_025277884.1
Accession Version	XM_025277884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase HECW2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACAGAGAAT 
ATGACCCTAC AGCGGGCCAA CAGTGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC 
TTGACGGCCA GCATGTATGA GTACACTCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TATATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC 
AAATACTACC ATGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT 
TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT 
AATCCAGTTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG 
ATTGAAATTA AAGACAAGTT TGCTAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG 
CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACAT CTTCTATTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA 
CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAAC ACAGTGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG 
GGAGCCTCTC CAAAGTTAAG GAGTAGTTTT CCCACTGATA CAAGACTCAA TGCAATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC 
TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC 
CTGGCATCTC AGACAAAGCC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA 
CCCTCAGGCC CGGTCCCAGA TGAAAGTGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC 
ACGGGCACTT CTGACACGTC TGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTC 
GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGCGA CCCTGCCAGG 
ACAGAGAGTG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA 
TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGCAGAAGG TGCTGCTCGG 
GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AGGGCGCGAT TGGAGGTTGT 
CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA CGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG 
CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT 
ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG 
AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG 
AGCAGCAGAC CCACGAGTGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC 
AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG 
TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA 
GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTACTGT 
CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG 
CAGAGAAATA AGCTGTACGT CACATTCGTT GGAGAGGAAG GGCTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCACT AATTCACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCA TTTTATAAGG CTCTTCTCAG AATTCTCTGT 
GACCTGAGTG ATCTAGAATA CCTTGATGAA GAGTTCCATC AGAGCCTGCA GTGGATGAAA 
GACAACGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAACGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAACA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATTGAGAG GATGGTGAAA TGGAGGATTG AGAGGGGTGT CGTGCAGCAG 
ACAGAGAGCT TAGTGCGTGG CTTCTATGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT GGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATACAG AGGAGGATAT CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTGA GGTTGTTACA GAGCTCACAC 
TTGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133669.1
CDS	150..4658
Translation	MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMTENMTLQRANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGNQHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPVWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFRVEVTSSIHEDASPEAVGTILGVSSVNGDLGSPSDEEDMPGGHHDSPVCSNGPVSEESADGTPKHSLRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHSGHSRSGAATCSERSVGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRTDDGSLASQTKPEDNPVETEEASINEAASFEDKPGNLPELAETSLPSGPVPDESENGPESQPSVDQGSTELCGSQEVDQPTSGADTGTSDTSGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQDSICTAGSLPVVQVPSGEEEGPGSESTTVPDQEEVGEVWQRRGSLEGAAAAAESQPQAEGAARDTPGACEGATAQEEGAIGGCQSNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENSNAIDGAGEEADFHQASADFRRESVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQSSHLF

Target ORF information:

RefSeq Version	XM_025277884.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025277884.1

ORF Insert Sequence:

ATGGCTAGCT CAGCCCGGGA GCACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACAGAGAAT 
ATGACCCTAC AGCGGGCCAA CAGTGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC 
TTGACGGCCA GCATGTATGA GTACACTCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG 
GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TATATCACAT AGATGAGAAT 
TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA 
ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC 
AAATACTACC ATGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG 
AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT 
TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGGCT AAAGAAAGGG 
ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT 
TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT 
AATCCAGTTT GGCACCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG 
ATTGAAATTA AAGACAAGTT TGCTAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG 
CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC 
TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG 
GAGGTCACAT CTTCTATTCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA 
GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC 
CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA 
CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT 
ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT 
GCTATTGAAC ACAGTGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG 
GGAGCCTCTC CAAAGTTAAG GAGTAGTTTT CCCACTGATA CAAGACTCAA TGCAATGCTT 
CATATTGACT CAGATGAAGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC 
TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC 
CTGGCATCTC AGACAAAGCC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT 
GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA 
CCCTCAGGCC CGGTCCCAGA TGAAAGTGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC 
CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC 
ACGGGCACTT CTGACACGTC TGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTC 
GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGCGA CCCTGCCAGG 
ACAGAGAGTG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCC 
GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA 
TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT 
GGAGCCTGTG CAGCAGAGCC CACCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA 
TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG 
TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG 
AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGCAGAAGG TGCTGCTCGG 
GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AGGGCGCGAT TGGAGGTTGT 
CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG 
TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC 
AGGATCTTCT ACGTGGATCA CGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC 
CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG 
CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT 
ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG 
AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC 
GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC 
CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC 
CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA 
AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA 
TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG 
AGCAGCAGAC CCACGAGTGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC 
AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG 
TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC 
AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT 
CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA 
GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA 
TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTACTGT 
CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC 
AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC 
AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA 
AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG 
CAGAGAAATA AGCTGTACGT CACATTCGTT GGAGAGGAAG GGCTGGATTA CAGTGGACCT 
TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT 
GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC 
AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GTCTTGCACT AATTCACCAG 
TATTTGTTGG ATGCCTTCTT CACACGGCCA TTTTATAAGG CTCTTCTCAG AATTCTCTGT 
GACCTGAGTG ATCTAGAATA CCTTGATGAA GAGTTCCATC AGAGCCTGCA GTGGATGAAA 
GACAACGACA TCCATGACAT CCTGGACCTC ACGTTCACTG TGAACGAAGA AGTTTTTGGG 
CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAACA TCCCAGTCAC AGAGAAGAAC 
AAGAAGGAGT ACATTGAGAG GATGGTGAAA TGGAGGATTG AGAGGGGTGT CGTGCAGCAG 
ACAGAGAGCT TAGTGCGTGG CTTCTATGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT 
GATGCAAGAG AACTAGAACT GGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGACTGG 
AGAAACAACA CAGAATACAG AGGAGGATAT CATGACAATC ACATTGTAAT TCGGTGGTTC 
TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTGA GGTTGTTACA GAGCTCACAC 
TTGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	HECW2
Entrez Gene ID	102399118
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

HECW2 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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