CSDE1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	CSDE1
Entrez Gene ID	102398731
Full Name	cold shock domain containing E1
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006064802.2	XP_006064864.1	cold shock domain-containing protein E1 isoform X1
XM_006064804.2	XP_006064866.1	cold shock domain-containing protein E1 isoform X3
XM_006064802.1	XP_006064864.1	cold shock domain-containing protein E1 isoform X1
XM_006064803.1	XP_006064865.1	cold shock domain-containing protein E1 isoform X2
XM_006064804.1	XP_006064866.1	cold shock domain-containing protein E1 isoform X3
XM_006064803.2	XP_006064865.1	cold shock domain-containing protein E1 isoform X2

Pathways from BioSystems
Gene Ontology

The following CSDE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CSDE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu20442	XM_006064802.2 Latest version!	Bubalus bubalis cold shock domain containing E1 (CSDE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu20443	XM_006064803.2 Latest version!	Bubalus bubalis cold shock domain containing E1 (CSDE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu20444	XM_006064804.2 Latest version!	Bubalus bubalis cold shock domain containing E1 (CSDE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OBu20442	XM_006064802.1	Bubalus bubalis cold shock domain containing E1, RNA-binding (CSDE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu20444	XM_006064804.1	Bubalus bubalis cold shock domain containing E1, RNA-binding (CSDE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu20443	XM_006064803.1	Bubalus bubalis cold shock domain containing E1, RNA-binding (CSDE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CSDE1 ( XM_006064802.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006064864.1 Bubalus bubalis cold shock domain containing E1 (CSDE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu20442
Clone ID Related Accession (Same CDS sequence)	XM_006064802.2 , XM_006064802.1
Accession Version	XM_006064802.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cold shock domain-containing protein E1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006064802.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG TTTTTACTGT GTCATCCGAT CCTCATCCCC CTCCTGCAGC CCCTCCTTCA 
CTTTCTTTAC CTTTATCTTC ATCTTCTACC TCATCTGGGA CTAAAAAACA AAAGAGGACC 
CCCACGTATC AGAGATCTAT GAGCTTTGAT CCAAACCTTC TCCACAACAA TGGACATAAT 
GGGTACCCCA ATGGTACTTC AGCAGCACTG CGTGAGACTG GGGTTATTGA AAAACTGCTA 
ACCTCTTACG GATTTATTCA GTGTTCAGAA CGGCAAGCTA GACTTTTCTT CCACTGTTCA 
CAGTATAATG GCAACCTGCA GGACTTAAAA GTAGGAGATG ATGTTGAATT TGAAGTATCA 
TCTGATCGGC GGACTGGGAA ACCCATAGCT GTTAAACTGG TGAAGATAAA ACAAGAAATC 
CTCCCTGAAG AACGAATTAA TGGACAAGTT GTGTGCGCTG TTCCTCACAA CTTAGAGAGT 
AAATCTCCAG CTGCCCCGGG TCAGAGTCCA ACAGGGAGTG TATGCTACGA ACGTAATGGG 
GAAGTGTTTT ATCTGACTTA TACCCCTGAA GATGTTGAAG GGAATGTTCA GCTGGAAACT 
GGAGATAAAA TAAACTTTGT AATTGATAAC AATAAACATA CTGGTGCTGT AAGTGCTCGT 
AATATTATGC TGTTGAAAAA GAAACAAGCT CGCTATCAGG GAGTAGTTTG TGCCATGAAA 
GAGGCGTTTG GCTTTATTGA AAGAGGCGAT ATTGTAAAGG AGATATTCTT TCACTATAGT 
GAATTTAAGG GTGACTTAGA ATCCTTACAG CCTGGAGATG ACGTGGAATT CACAATCAAG 
GACCGAAATG GTAAAGAAGT TGCAACAGAT GTCAGACTGT TGCCTCAAGG AACAGTCATT 
TTTGAAGATA TCAGCATTGA ACATTTTGAA GGAACTGTAA CCAAAGTCAT CCCCAAAGTA 
CCCAGTAAAA ACCAGAATGA CCCATTGCCA GGACGTATCA AAGTTGATTT TGTGATTCCT 
AAAGAACTTC CCTTTGGAGA CAAAGATACA AAATCCAAGG TGACCCTGTT GGAAGGTGAC 
CACGTTAGGT TTAATATTTC AACAGACCGT CGTGACAAAT TAGAACGAGC AACCAACATA 
GAAGTTCTAT CAAATACATT TCAGTTCACT AATGAAGCCA GAGAGATGGG TGTAATTGCT 
GCCATGAGAG ATGGTTTTGG TTTCATCAAG TGTGTGGATC GTGATGCTCG TATGTTCTTC 
CACTTCAGTG AAATTCTGGA TGGGAACCAG CTTCATATTG CAGATGAAGT AGAGTTTACT 
GTGGTTCCTG ATATGCTCTC TGCCCAAAGA AATCATGCTA TTAGGATTAA AAAACTTCCC 
AAGGGCACGG TTTCGTTCCA CTCCCATTCA GATCATCGTT TTCTGGGCAC TGTAGAAAAA 
GAGGCCACTT TTTCGAATCC TAAAACCACT AGCCCAAATA AAGGCAAAGA AAAGGAGGCT 
GAGGATGGCA TTATTGCTTA TGATGATTGT GGGGTGAAAT TGACTATTGC TTTTCAAGCC 
AAGGATGTGG AAGGATCTAC TTCTCCTCAA ATAGGAGACA AGGTTGAATT TAGTATTAGT 
GACAAACAGA GGCCTGGACA GCAGATTGCA ACTTGTGTGC GACTCTTAGG TCGTAATTCG 
AACTCCAAGA GGCTCTTGGG TTATGTGGCA ACTTTGAAGG ATAATTTTGG ATTTATTGAA 
ACAGCCAATC ATGATAAGGA AATCTTTTTC CATTACAGTG AGTTCTCTGG TGATGTTGAT 
AGCCTGGAAC TGGGGGACAT GGTCGAGTAC AGCTTGTCCA AAGGAAAAGG CAACAAAGTC 
AGTGCAGAAA AAGTGAACAA AACACACTCA GTGAATGGCA TTACTGAGGA AGCTGATCCC 
ACCATCTACT CTGGTAAAGT CATTCGCCCC TTGAGGAGTG TTGATCCAAC ACAGAATGAG 
TACCAAGGAA TGATTGAGAT CGTGGACGAA GGGGATATGA AAGGTGAGGT CTATCCATTT 
GGCATAGTTG GGATGGCCAA CAAAGGGGAT TGCCTACAGA AAGGGGAGAG TGTCAAGTTC 
CAGTTGTGTG TCCTGGGCCA AAACGCACAG ACTATGGCCT ACAACATCAC ACCCCTGCGT 
AGGGCTACAG TGGAGTGTGT GAAAGATCAG TTTGGTTTCA TTAACTATGA AGTAGGAGAT 
AGCAAGAAGC TCTTTTTCCA TGTGAAAGAA GTTCAGGATG GCATTGAGCT ACAAGCAGGA 
GATGAGGTGG AATTCTCAGT GATTCTTAAT CAGCGCACTG GCAAGTGCAG TGCTTGTAAT 
GTTTGGCGAG TCTGCGAGGG CCCCAAGGCT GTTGCAGCTC CACGACCTGA TAGGCTGGTC 
AATCGCTTGA AGAATATCAC CCTGGATGAT GCCAGTGCTC CTCGCCTAAT GGTTCTTCGT 
CAGCCAAGGG GACCAGATAA CTCAATGGGA TTTGGTGCAG AAAGAAAGAT CCGTCAAGCT 
GGTGTGATTG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006064864.1
CDS	550..3084
Translation	MENVFTVSSDPHPPPAAPPSLSLPLSSSSTSSGTKKQKRTPTYQRSMSFDPNLLHNNGHNGYPNGTSAALRETGVIEKLLTSYGFIQCSERQARLFFHCSQYNGNLQDLKVGDDVEFEVSSDRRTGKPIAVKLVKIKQEILPEERINGQVVCAVPHNLESKSPAAPGQSPTGSVCYERNGEVFYLTYTPEDVEGNVQLETGDKINFVIDNNKHTGAVSARNIMLLKKKQARYQGVVCAMKEAFGFIERGDIVKEIFFHYSEFKGDLESLQPGDDVEFTIKDRNGKEVATDVRLLPQGTVIFEDISIEHFEGTVTKVIPKVPSKNQNDPLPGRIKVDFVIPKELPFGDKDTKSKVTLLEGDHVRFNISTDRRDKLERATNIEVLSNTFQFTNEAREMGVIAAMRDGFGFIKCVDRDARMFFHFSEILDGNQLHIADEVEFTVVPDMLSAQRNHAIRIKKLPKGTVSFHSHSDHRFLGTVEKEATFSNPKTTSPNKGKEKEAEDGIIAYDDCGVKLTIAFQAKDVEGSTSPQIGDKVEFSISDKQRPGQQIATCVRLLGRNSNSKRLLGYVATLKDNFGFIETANHDKEIFFHYSEFSGDVDSLELGDMVEYSLSKGKGNKVSAEKVNKTHSVNGITEEADPTIYSGKVIRPLRSVDPTQNEYQGMIEIVDEGDMKGEVYPFGIVGMANKGDCLQKGESVKFQLCVLGQNAQTMAYNITPLRRATVECVKDQFGFINYEVGDSKKLFFHVKEVQDGIELQAGDEVEFSVILNQRTGKCSACNVWRVCEGPKAVAAPRPDRLVNRLKNITLDDASAPRLMVLRQPRGPDNSMGFGAERKIRQAGVID

Target ORF information:

RefSeq Version	XM_006064802.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cold shock domain containing E1 (CSDE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064802.2

ORF Insert Sequence:

ATGGAGAACG TTTTTACTGT GTCATCCGAT CCTCATCCCC CTCCTGCAGC CCCTCCTTCA 
CTTTCTTTAC CTTTATCTTC ATCTTCTACC TCATCTGGGA CTAAAAAACA AAAGAGGACC 
CCCACGTATC AGAGATCTAT GAGCTTTGAT CCAAACCTTC TCCACAACAA TGGACATAAT 
GGGTACCCCA ATGGTACTTC AGCAGCACTG CGTGAGACTG GGGTTATTGA AAAACTGCTA 
ACCTCTTACG GATTTATTCA GTGTTCAGAA CGGCAAGCTA GACTTTTCTT CCACTGTTCA 
CAGTATAATG GCAACCTGCA GGACTTAAAA GTAGGAGATG ATGTTGAATT TGAAGTATCA 
TCTGATCGGC GGACTGGGAA ACCCATAGCT GTTAAACTGG TGAAGATAAA ACAAGAAATC 
CTCCCTGAAG AACGAATTAA TGGACAAGTT GTGTGCGCTG TTCCTCACAA CTTAGAGAGT 
AAATCTCCAG CTGCCCCGGG TCAGAGTCCA ACAGGGAGTG TATGCTACGA ACGTAATGGG 
GAAGTGTTTT ATCTGACTTA TACCCCTGAA GATGTTGAAG GGAATGTTCA GCTGGAAACT 
GGAGATAAAA TAAACTTTGT AATTGATAAC AATAAACATA CTGGTGCTGT AAGTGCTCGT 
AATATTATGC TGTTGAAAAA GAAACAAGCT CGCTATCAGG GAGTAGTTTG TGCCATGAAA 
GAGGCGTTTG GCTTTATTGA AAGAGGCGAT ATTGTAAAGG AGATATTCTT TCACTATAGT 
GAATTTAAGG GTGACTTAGA ATCCTTACAG CCTGGAGATG ACGTGGAATT CACAATCAAG 
GACCGAAATG GTAAAGAAGT TGCAACAGAT GTCAGACTGT TGCCTCAAGG AACAGTCATT 
TTTGAAGATA TCAGCATTGA ACATTTTGAA GGAACTGTAA CCAAAGTCAT CCCCAAAGTA 
CCCAGTAAAA ACCAGAATGA CCCATTGCCA GGACGTATCA AAGTTGATTT TGTGATTCCT 
AAAGAACTTC CCTTTGGAGA CAAAGATACA AAATCCAAGG TGACCCTGTT GGAAGGTGAC 
CACGTTAGGT TTAATATTTC AACAGACCGT CGTGACAAAT TAGAACGAGC AACCAACATA 
GAAGTTCTAT CAAATACATT TCAGTTCACT AATGAAGCCA GAGAGATGGG TGTAATTGCT 
GCCATGAGAG ATGGTTTTGG TTTCATCAAG TGTGTGGATC GTGATGCTCG TATGTTCTTC 
CACTTCAGTG AAATTCTGGA TGGGAACCAG CTTCATATTG CAGATGAAGT AGAGTTTACT 
GTGGTTCCTG ATATGCTCTC TGCCCAAAGA AATCATGCTA TTAGGATTAA AAAACTTCCC 
AAGGGCACGG TTTCGTTCCA CTCCCATTCA GATCATCGTT TTCTGGGCAC TGTAGAAAAA 
GAGGCCACTT TTTCGAATCC TAAAACCACT AGCCCAAATA AAGGCAAAGA AAAGGAGGCT 
GAGGATGGCA TTATTGCTTA TGATGATTGT GGGGTGAAAT TGACTATTGC TTTTCAAGCC 
AAGGATGTGG AAGGATCTAC TTCTCCTCAA ATAGGAGACA AGGTTGAATT TAGTATTAGT 
GACAAACAGA GGCCTGGACA GCAGATTGCA ACTTGTGTGC GACTCTTAGG TCGTAATTCG 
AACTCCAAGA GGCTCTTGGG TTATGTGGCA ACTTTGAAGG ATAATTTTGG ATTTATTGAA 
ACAGCCAATC ATGATAAGGA AATCTTTTTC CATTACAGTG AGTTCTCTGG TGATGTTGAT 
AGCCTGGAAC TGGGGGACAT GGTCGAGTAC AGCTTGTCCA AAGGAAAAGG CAACAAAGTC 
AGTGCAGAAA AAGTGAACAA AACACACTCA GTGAATGGCA TTACTGAGGA AGCTGATCCC 
ACCATCTACT CTGGTAAAGT CATTCGCCCC TTGAGGAGTG TTGATCCAAC ACAGAATGAG 
TACCAAGGAA TGATTGAGAT CGTGGACGAA GGGGATATGA AAGGTGAGGT CTATCCATTT 
GGCATAGTTG GGATGGCCAA CAAAGGGGAT TGCCTACAGA AAGGGGAGAG TGTCAAGTTC 
CAGTTGTGTG TCCTGGGCCA AAACGCACAG ACTATGGCCT ACAACATCAC ACCCCTGCGT 
AGGGCTACAG TGGAGTGTGT GAAAGATCAG TTTGGTTTCA TTAACTATGA AGTAGGAGAT 
AGCAAGAAGC TCTTTTTCCA TGTGAAAGAA GTTCAGGATG GCATTGAGCT ACAAGCAGGA 
GATGAGGTGG AATTCTCAGT GATTCTTAAT CAGCGCACTG GCAAGTGCAG TGCTTGTAAT 
GTTTGGCGAG TCTGCGAGGG CCCCAAGGCT GTTGCAGCTC CACGACCTGA TAGGCTGGTC 
AATCGCTTGA AGAATATCAC CCTGGATGAT GCCAGTGCTC CTCGCCTAAT GGTTCTTCGT 
CAGCCAAGGG GACCAGATAA CTCAATGGGA TTTGGTGCAG AAAGAAAGAT CCGTCAAGCT 
GGTGTGATTG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CSDE1 ( XM_006064802.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006064864.1 Bubalus bubalis cold shock domain containing E1, RNA-binding (CSDE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu20442
Clone ID Related Accession (Same CDS sequence)	XM_006064802.2 , XM_006064802.1
Accession Version	XM_006064802.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	cold shock domain-containing protein E1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785149.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG TTTTTACTGT GTCATCCGAT CCTCATCCCC CTCCTGCAGC CCCTCCTTCA 
CTTTCTTTAC CTTTATCTTC ATCTTCTACC TCATCTGGGA CTAAAAAACA AAAGAGGACC 
CCCACGTATC AGAGATCTAT GAGCTTTGAT CCAAACCTTC TCCACAACAA TGGACATAAT 
GGGTACCCCA ATGGTACTTC AGCAGCACTG CGTGAGACTG GGGTTATTGA AAAACTGCTA 
ACCTCTTACG GATTTATTCA GTGTTCAGAA CGGCAAGCTA GACTTTTCTT CCACTGTTCA 
CAGTATAATG GCAACCTGCA GGACTTAAAA GTAGGAGATG ATGTTGAATT TGAAGTATCA 
TCTGATCGGC GGACTGGGAA ACCCATAGCT GTTAAACTGG TGAAGATAAA ACAAGAAATC 
CTCCCTGAAG AACGAATTAA TGGACAAGTT GTGTGCGCTG TTCCTCACAA CTTAGAGAGT 
AAATCTCCAG CTGCCCCGGG TCAGAGTCCA ACAGGGAGTG TATGCTACGA ACGTAATGGG 
GAAGTGTTTT ATCTGACTTA TACCCCTGAA GATGTTGAAG GGAATGTTCA GCTGGAAACT 
GGAGATAAAA TAAACTTTGT AATTGATAAC AATAAACATA CTGGTGCTGT AAGTGCTCGT 
AATATTATGC TGTTGAAAAA GAAACAAGCT CGCTATCAGG GAGTAGTTTG TGCCATGAAA 
GAGGCGTTTG GCTTTATTGA AAGAGGCGAT ATTGTAAAGG AGATATTCTT TCACTATAGT 
GAATTTAAGG GTGACTTAGA ATCCTTACAG CCTGGAGATG ACGTGGAATT CACAATCAAG 
GACCGAAATG GTAAAGAAGT TGCAACAGAT GTCAGACTGT TGCCTCAAGG AACAGTCATT 
TTTGAAGATA TCAGCATTGA ACATTTTGAA GGAACTGTAA CCAAAGTCAT CCCCAAAGTA 
CCCAGTAAAA ACCAGAATGA CCCATTGCCA GGACGTATCA AAGTTGATTT TGTGATTCCT 
AAAGAACTTC CCTTTGGAGA CAAAGATACA AAATCCAAGG TGACCCTGTT GGAAGGTGAC 
CACGTTAGGT TTAATATTTC AACAGACCGT CGTGACAAAT TAGAACGAGC AACCAACATA 
GAAGTTCTAT CAAATACATT TCAGTTCACT AATGAAGCCA GAGAGATGGG TGTAATTGCT 
GCCATGAGAG ATGGTTTTGG TTTCATCAAG TGTGTGGATC GTGATGCTCG TATGTTCTTC 
CACTTCAGTG AAATTCTGGA TGGGAACCAG CTTCATATTG CAGATGAAGT AGAGTTTACT 
GTGGTTCCTG ATATGCTCTC TGCCCAAAGA AATCATGCTA TTAGGATTAA AAAACTTCCC 
AAGGGCACGG TTTCGTTCCA CTCCCATTCA GATCATCGTT TTCTGGGCAC TGTAGAAAAA 
GAGGCCACTT TTTCGAATCC TAAAACCACT AGCCCAAATA AAGGCAAAGA AAAGGAGGCT 
GAGGATGGCA TTATTGCTTA TGATGATTGT GGGGTGAAAT TGACTATTGC TTTTCAAGCC 
AAGGATGTGG AAGGATCTAC TTCTCCTCAA ATAGGAGACA AGGTTGAATT TAGTATTAGT 
GACAAACAGA GGCCTGGACA GCAGATTGCA ACTTGTGTGC GACTCTTAGG TCGTAATTCG 
AACTCCAAGA GGCTCTTGGG TTATGTGGCA ACTTTGAAGG ATAATTTTGG ATTTATTGAA 
ACAGCCAATC ATGATAAGGA AATCTTTTTC CATTACAGTG AGTTCTCTGG TGATGTTGAT 
AGCCTGGAAC TGGGGGACAT GGTCGAGTAC AGCTTGTCCA AAGGAAAAGG CAACAAAGTC 
AGTGCAGAAA AAGTGAACAA AACACACTCA GTGAATGGCA TTACTGAGGA AGCTGATCCC 
ACCATCTACT CTGGTAAAGT CATTCGCCCC TTGAGGAGTG TTGATCCAAC ACAGAATGAG 
TACCAAGGAA TGATTGAGAT CGTGGACGAA GGGGATATGA AAGGTGAGGT CTATCCATTT 
GGCATAGTTG GGATGGCCAA CAAAGGGGAT TGCCTACAGA AAGGGGAGAG TGTCAAGTTC 
CAGTTGTGTG TCCTGGGCCA AAACGCACAG ACTATGGCCT ACAACATCAC ACCCCTGCGT 
AGGGCTACAG TGGAGTGTGT GAAAGATCAG TTTGGTTTCA TTAACTATGA AGTAGGAGAT 
AGCAAGAAGC TCTTTTTCCA TGTGAAAGAA GTTCAGGATG GCATTGAGCT ACAAGCAGGA 
GATGAGGTGG AATTCTCAGT GATTCTTAAT CAGCGCACTG GCAAGTGCAG TGCTTGTAAT 
GTTTGGCGAG TCTGCGAGGG CCCCAAGGCT GTTGCAGCTC CACGACCTGA TAGGCTGGTC 
AATCGCTTGA AGAATATCAC CCTGGATGAT GCCAGTGCTC CTCGCCTAAT GGTTCTTCGT 
CAGCCAAGGG GACCAGATAA CTCAATGGGA TTTGGTGCAG AAAGAAAGAT CCGTCAAGCT 
GGTGTGATTG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006064864.1
CDS	515..3049
Translation	MENVFTVSSDPHPPPAAPPSLSLPLSSSSTSSGTKKQKRTPTYQRSMSFDPNLLHNNGHNGYPNGTSAALRETGVIEKLLTSYGFIQCSERQARLFFHCSQYNGNLQDLKVGDDVEFEVSSDRRTGKPIAVKLVKIKQEILPEERINGQVVCAVPHNLESKSPAAPGQSPTGSVCYERNGEVFYLTYTPEDVEGNVQLETGDKINFVIDNNKHTGAVSARNIMLLKKKQARYQGVVCAMKEAFGFIERGDIVKEIFFHYSEFKGDLESLQPGDDVEFTIKDRNGKEVATDVRLLPQGTVIFEDISIEHFEGTVTKVIPKVPSKNQNDPLPGRIKVDFVIPKELPFGDKDTKSKVTLLEGDHVRFNISTDRRDKLERATNIEVLSNTFQFTNEAREMGVIAAMRDGFGFIKCVDRDARMFFHFSEILDGNQLHIADEVEFTVVPDMLSAQRNHAIRIKKLPKGTVSFHSHSDHRFLGTVEKEATFSNPKTTSPNKGKEKEAEDGIIAYDDCGVKLTIAFQAKDVEGSTSPQIGDKVEFSISDKQRPGQQIATCVRLLGRNSNSKRLLGYVATLKDNFGFIETANHDKEIFFHYSEFSGDVDSLELGDMVEYSLSKGKGNKVSAEKVNKTHSVNGITEEADPTIYSGKVIRPLRSVDPTQNEYQGMIEIVDEGDMKGEVYPFGIVGMANKGDCLQKGESVKFQLCVLGQNAQTMAYNITPLRRATVECVKDQFGFINYEVGDSKKLFFHVKEVQDGIELQAGDEVEFSVILNQRTGKCSACNVWRVCEGPKAVAAPRPDRLVNRLKNITLDDASAPRLMVLRQPRGPDNSMGFGAERKIRQAGVID

Target ORF information:

RefSeq Version	XM_006064802.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cold shock domain containing E1, RNA-binding (CSDE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064802.1

ORF Insert Sequence:

ATGGAGAACG TTTTTACTGT GTCATCCGAT CCTCATCCCC CTCCTGCAGC CCCTCCTTCA 
CTTTCTTTAC CTTTATCTTC ATCTTCTACC TCATCTGGGA CTAAAAAACA AAAGAGGACC 
CCCACGTATC AGAGATCTAT GAGCTTTGAT CCAAACCTTC TCCACAACAA TGGACATAAT 
GGGTACCCCA ATGGTACTTC AGCAGCACTG CGTGAGACTG GGGTTATTGA AAAACTGCTA 
ACCTCTTACG GATTTATTCA GTGTTCAGAA CGGCAAGCTA GACTTTTCTT CCACTGTTCA 
CAGTATAATG GCAACCTGCA GGACTTAAAA GTAGGAGATG ATGTTGAATT TGAAGTATCA 
TCTGATCGGC GGACTGGGAA ACCCATAGCT GTTAAACTGG TGAAGATAAA ACAAGAAATC 
CTCCCTGAAG AACGAATTAA TGGACAAGTT GTGTGCGCTG TTCCTCACAA CTTAGAGAGT 
AAATCTCCAG CTGCCCCGGG TCAGAGTCCA ACAGGGAGTG TATGCTACGA ACGTAATGGG 
GAAGTGTTTT ATCTGACTTA TACCCCTGAA GATGTTGAAG GGAATGTTCA GCTGGAAACT 
GGAGATAAAA TAAACTTTGT AATTGATAAC AATAAACATA CTGGTGCTGT AAGTGCTCGT 
AATATTATGC TGTTGAAAAA GAAACAAGCT CGCTATCAGG GAGTAGTTTG TGCCATGAAA 
GAGGCGTTTG GCTTTATTGA AAGAGGCGAT ATTGTAAAGG AGATATTCTT TCACTATAGT 
GAATTTAAGG GTGACTTAGA ATCCTTACAG CCTGGAGATG ACGTGGAATT CACAATCAAG 
GACCGAAATG GTAAAGAAGT TGCAACAGAT GTCAGACTGT TGCCTCAAGG AACAGTCATT 
TTTGAAGATA TCAGCATTGA ACATTTTGAA GGAACTGTAA CCAAAGTCAT CCCCAAAGTA 
CCCAGTAAAA ACCAGAATGA CCCATTGCCA GGACGTATCA AAGTTGATTT TGTGATTCCT 
AAAGAACTTC CCTTTGGAGA CAAAGATACA AAATCCAAGG TGACCCTGTT GGAAGGTGAC 
CACGTTAGGT TTAATATTTC AACAGACCGT CGTGACAAAT TAGAACGAGC AACCAACATA 
GAAGTTCTAT CAAATACATT TCAGTTCACT AATGAAGCCA GAGAGATGGG TGTAATTGCT 
GCCATGAGAG ATGGTTTTGG TTTCATCAAG TGTGTGGATC GTGATGCTCG TATGTTCTTC 
CACTTCAGTG AAATTCTGGA TGGGAACCAG CTTCATATTG CAGATGAAGT AGAGTTTACT 
GTGGTTCCTG ATATGCTCTC TGCCCAAAGA AATCATGCTA TTAGGATTAA AAAACTTCCC 
AAGGGCACGG TTTCGTTCCA CTCCCATTCA GATCATCGTT TTCTGGGCAC TGTAGAAAAA 
GAGGCCACTT TTTCGAATCC TAAAACCACT AGCCCAAATA AAGGCAAAGA AAAGGAGGCT 
GAGGATGGCA TTATTGCTTA TGATGATTGT GGGGTGAAAT TGACTATTGC TTTTCAAGCC 
AAGGATGTGG AAGGATCTAC TTCTCCTCAA ATAGGAGACA AGGTTGAATT TAGTATTAGT 
GACAAACAGA GGCCTGGACA GCAGATTGCA ACTTGTGTGC GACTCTTAGG TCGTAATTCG 
AACTCCAAGA GGCTCTTGGG TTATGTGGCA ACTTTGAAGG ATAATTTTGG ATTTATTGAA 
ACAGCCAATC ATGATAAGGA AATCTTTTTC CATTACAGTG AGTTCTCTGG TGATGTTGAT 
AGCCTGGAAC TGGGGGACAT GGTCGAGTAC AGCTTGTCCA AAGGAAAAGG CAACAAAGTC 
AGTGCAGAAA AAGTGAACAA AACACACTCA GTGAATGGCA TTACTGAGGA AGCTGATCCC 
ACCATCTACT CTGGTAAAGT CATTCGCCCC TTGAGGAGTG TTGATCCAAC ACAGAATGAG 
TACCAAGGAA TGATTGAGAT CGTGGACGAA GGGGATATGA AAGGTGAGGT CTATCCATTT 
GGCATAGTTG GGATGGCCAA CAAAGGGGAT TGCCTACAGA AAGGGGAGAG TGTCAAGTTC 
CAGTTGTGTG TCCTGGGCCA AAACGCACAG ACTATGGCCT ACAACATCAC ACCCCTGCGT 
AGGGCTACAG TGGAGTGTGT GAAAGATCAG TTTGGTTTCA TTAACTATGA AGTAGGAGAT 
AGCAAGAAGC TCTTTTTCCA TGTGAAAGAA GTTCAGGATG GCATTGAGCT ACAAGCAGGA 
GATGAGGTGG AATTCTCAGT GATTCTTAAT CAGCGCACTG GCAAGTGCAG TGCTTGTAAT 
GTTTGGCGAG TCTGCGAGGG CCCCAAGGCT GTTGCAGCTC CACGACCTGA TAGGCTGGTC 
AATCGCTTGA AGAATATCAC CCTGGATGAT GCCAGTGCTC CTCGCCTAAT GGTTCTTCGT 
CAGCCAAGGG GACCAGATAA CTCAATGGGA TTTGGTGCAG AAAGAAAGAT CCGTCAAGCT 
GGTGTGATTG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CSDE1 ( XM_006064803.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006064865.1 Bubalus bubalis cold shock domain containing E1, RNA-binding (CSDE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu20443
Clone ID Related Accession (Same CDS sequence)	XM_006064803.1 , XM_006064803.2
Accession Version	XM_006064803.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	cold shock domain-containing protein E1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785149.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG TTTTTACTGT GTCATCCGAT CCTCATCCCC CTCCTGCAGC CCCTCCTTCA 
CTTTCTTTAC CTTTATCTTC ATCTTCTACC TCATCTGGGA CTAAAAAACA AAAGAGGACC 
CCCACGTATC AGAGATCTAT GAGCTTTGAT CCAAACCTTC TCCACAACAA TGGACATAAT 
GGGTACCCCA ATGGTACTTC AGCAGCACTG CGTGAGACTG GGGTTATTGA AAAACTGCTA 
ACCTCTTACG GATTTATTCA GTGTTCAGAA CGGCAAGCTA GACTTTTCTT CCACTGTTCA 
CAGTATAATG GCAACCTGCA GGACTTAAAA GTAGGAGATG ATGTTGAATT TGAAGTATCA 
TCTGATCGGC GGACTGGGAA ACCCATAGCT GTTAAACTGG TGAAGATAAA ACAAGAAATC 
CTCCCTGAAG AACGAATTAA TGGACAAGAA GTGTTTTATC TGACTTATAC CCCTGAAGAT 
GTTGAAGGGA ATGTTCAGCT GGAAACTGGA GATAAAATAA ACTTTGTAAT TGATAACAAT 
AAACATACTG GTGCTGTAAG TGCTCGTAAT ATTATGCTGT TGAAAAAGAA ACAAGCTCGC 
TATCAGGGAG TAGTTTGTGC CATGAAAGAG GCGTTTGGCT TTATTGAAAG AGGCGATATT 
GTAAAGGAGA TATTCTTTCA CTATAGTGAA TTTAAGGGTG ACTTAGAATC CTTACAGCCT 
GGAGATGACG TGGAATTCAC AATCAAGGAC CGAAATGGTA AAGAAGTTGC AACAGATGTC 
AGACTGTTGC CTCAAGGAAC AGTCATTTTT GAAGATATCA GCATTGAACA TTTTGAAGGA 
ACTGTAACCA AAGTCATCCC CAAAGTACCC AGTAAAAACC AGAATGACCC ATTGCCAGGA 
CGTATCAAAG TTGATTTTGT GATTCCTAAA GAACTTCCCT TTGGAGACAA AGATACAAAA 
TCCAAGGTGA CCCTGTTGGA AGGTGACCAC GTTAGGTTTA ATATTTCAAC AGACCGTCGT 
GACAAATTAG AACGAGCAAC CAACATAGAA GTTCTATCAA ATACATTTCA GTTCACTAAT 
GAAGCCAGAG AGATGGGTGT AATTGCTGCC ATGAGAGATG GTTTTGGTTT CATCAAGTGT 
GTGGATCGTG ATGCTCGTAT GTTCTTCCAC TTCAGTGAAA TTCTGGATGG GAACCAGCTT 
CATATTGCAG ATGAAGTAGA GTTTACTGTG GTTCCTGATA TGCTCTCTGC CCAAAGAAAT 
CATGCTATTA GGATTAAAAA ACTTCCCAAG GGCACGGTTT CGTTCCACTC CCATTCAGAT 
CATCGTTTTC TGGGCACTGT AGAAAAAGAG GCCACTTTTT CGAATCCTAA AACCACTAGC 
CCAAATAAAG GCAAAGAAAA GGAGGCTGAG GATGGCATTA TTGCTTATGA TGATTGTGGG 
GTGAAATTGA CTATTGCTTT TCAAGCCAAG GATGTGGAAG GATCTACTTC TCCTCAAATA 
GGAGACAAGG TTGAATTTAG TATTAGTGAC AAACAGAGGC CTGGACAGCA GATTGCAACT 
TGTGTGCGAC TCTTAGGTCG TAATTCGAAC TCCAAGAGGC TCTTGGGTTA TGTGGCAACT 
TTGAAGGATA ATTTTGGATT TATTGAAACA GCCAATCATG ATAAGGAAAT CTTTTTCCAT 
TACAGTGAGT TCTCTGGTGA TGTTGATAGC CTGGAACTGG GGGACATGGT CGAGTACAGC 
TTGTCCAAAG GAAAAGGCAA CAAAGTCAGT GCAGAAAAAG TGAACAAAAC ACACTCAGTG 
AATGGCATTA CTGAGGAAGC TGATCCCACC ATCTACTCTG GTAAAGTCAT TCGCCCCTTG 
AGGAGTGTTG ATCCAACACA GAATGAGTAC CAAGGAATGA TTGAGATCGT GGACGAAGGG 
GATATGAAAG GTGAGGTCTA TCCATTTGGC ATAGTTGGGA TGGCCAACAA AGGGGATTGC 
CTACAGAAAG GGGAGAGTGT CAAGTTCCAG TTGTGTGTCC TGGGCCAAAA CGCACAGACT 
ATGGCCTACA ACATCACACC CCTGCGTAGG GCTACAGTGG AGTGTGTGAA AGATCAGTTT 
GGTTTCATTA ACTATGAAGT AGGAGATAGC AAGAAGCTCT TTTTCCATGT GAAAGAAGTT 
CAGGATGGCA TTGAGCTACA AGCAGGAGAT GAGGTGGAAT TCTCAGTGAT TCTTAATCAG 
CGCACTGGCA AGTGCAGTGC TTGTAATGTT TGGCGAGTCT GCGAGGGCCC CAAGGCTGTT 
GCAGCTCCAC GACCTGATAG GCTGGTCAAT CGCTTGAAGA ATATCACCCT GGATGATGCC 
AGTGCTCCTC GCCTAATGGT TCTTCGTCAG CCAAGGGGAC CAGATAACTC AATGGGATTT 
GGTGCAGAAA GAAAGATCCG TCAAGCTGGT GTGATTGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006064865.1
CDS	514..2955
Translation	MENVFTVSSDPHPPPAAPPSLSLPLSSSSTSSGTKKQKRTPTYQRSMSFDPNLLHNNGHNGYPNGTSAALRETGVIEKLLTSYGFIQCSERQARLFFHCSQYNGNLQDLKVGDDVEFEVSSDRRTGKPIAVKLVKIKQEILPEERINGQEVFYLTYTPEDVEGNVQLETGDKINFVIDNNKHTGAVSARNIMLLKKKQARYQGVVCAMKEAFGFIERGDIVKEIFFHYSEFKGDLESLQPGDDVEFTIKDRNGKEVATDVRLLPQGTVIFEDISIEHFEGTVTKVIPKVPSKNQNDPLPGRIKVDFVIPKELPFGDKDTKSKVTLLEGDHVRFNISTDRRDKLERATNIEVLSNTFQFTNEAREMGVIAAMRDGFGFIKCVDRDARMFFHFSEILDGNQLHIADEVEFTVVPDMLSAQRNHAIRIKKLPKGTVSFHSHSDHRFLGTVEKEATFSNPKTTSPNKGKEKEAEDGIIAYDDCGVKLTIAFQAKDVEGSTSPQIGDKVEFSISDKQRPGQQIATCVRLLGRNSNSKRLLGYVATLKDNFGFIETANHDKEIFFHYSEFSGDVDSLELGDMVEYSLSKGKGNKVSAEKVNKTHSVNGITEEADPTIYSGKVIRPLRSVDPTQNEYQGMIEIVDEGDMKGEVYPFGIVGMANKGDCLQKGESVKFQLCVLGQNAQTMAYNITPLRRATVECVKDQFGFINYEVGDSKKLFFHVKEVQDGIELQAGDEVEFSVILNQRTGKCSACNVWRVCEGPKAVAAPRPDRLVNRLKNITLDDASAPRLMVLRQPRGPDNSMGFGAERKIRQAGVID

Target ORF information:

RefSeq Version	XM_006064803.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cold shock domain containing E1, RNA-binding (CSDE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064803.1

ORF Insert Sequence:

ATGGAGAACG TTTTTACTGT GTCATCCGAT CCTCATCCCC CTCCTGCAGC CCCTCCTTCA 
CTTTCTTTAC CTTTATCTTC ATCTTCTACC TCATCTGGGA CTAAAAAACA AAAGAGGACC 
CCCACGTATC AGAGATCTAT GAGCTTTGAT CCAAACCTTC TCCACAACAA TGGACATAAT 
GGGTACCCCA ATGGTACTTC AGCAGCACTG CGTGAGACTG GGGTTATTGA AAAACTGCTA 
ACCTCTTACG GATTTATTCA GTGTTCAGAA CGGCAAGCTA GACTTTTCTT CCACTGTTCA 
CAGTATAATG GCAACCTGCA GGACTTAAAA GTAGGAGATG ATGTTGAATT TGAAGTATCA 
TCTGATCGGC GGACTGGGAA ACCCATAGCT GTTAAACTGG TGAAGATAAA ACAAGAAATC 
CTCCCTGAAG AACGAATTAA TGGACAAGAA GTGTTTTATC TGACTTATAC CCCTGAAGAT 
GTTGAAGGGA ATGTTCAGCT GGAAACTGGA GATAAAATAA ACTTTGTAAT TGATAACAAT 
AAACATACTG GTGCTGTAAG TGCTCGTAAT ATTATGCTGT TGAAAAAGAA ACAAGCTCGC 
TATCAGGGAG TAGTTTGTGC CATGAAAGAG GCGTTTGGCT TTATTGAAAG AGGCGATATT 
GTAAAGGAGA TATTCTTTCA CTATAGTGAA TTTAAGGGTG ACTTAGAATC CTTACAGCCT 
GGAGATGACG TGGAATTCAC AATCAAGGAC CGAAATGGTA AAGAAGTTGC AACAGATGTC 
AGACTGTTGC CTCAAGGAAC AGTCATTTTT GAAGATATCA GCATTGAACA TTTTGAAGGA 
ACTGTAACCA AAGTCATCCC CAAAGTACCC AGTAAAAACC AGAATGACCC ATTGCCAGGA 
CGTATCAAAG TTGATTTTGT GATTCCTAAA GAACTTCCCT TTGGAGACAA AGATACAAAA 
TCCAAGGTGA CCCTGTTGGA AGGTGACCAC GTTAGGTTTA ATATTTCAAC AGACCGTCGT 
GACAAATTAG AACGAGCAAC CAACATAGAA GTTCTATCAA ATACATTTCA GTTCACTAAT 
GAAGCCAGAG AGATGGGTGT AATTGCTGCC ATGAGAGATG GTTTTGGTTT CATCAAGTGT 
GTGGATCGTG ATGCTCGTAT GTTCTTCCAC TTCAGTGAAA TTCTGGATGG GAACCAGCTT 
CATATTGCAG ATGAAGTAGA GTTTACTGTG GTTCCTGATA TGCTCTCTGC CCAAAGAAAT 
CATGCTATTA GGATTAAAAA ACTTCCCAAG GGCACGGTTT CGTTCCACTC CCATTCAGAT 
CATCGTTTTC TGGGCACTGT AGAAAAAGAG GCCACTTTTT CGAATCCTAA AACCACTAGC 
CCAAATAAAG GCAAAGAAAA GGAGGCTGAG GATGGCATTA TTGCTTATGA TGATTGTGGG 
GTGAAATTGA CTATTGCTTT TCAAGCCAAG GATGTGGAAG GATCTACTTC TCCTCAAATA 
GGAGACAAGG TTGAATTTAG TATTAGTGAC AAACAGAGGC CTGGACAGCA GATTGCAACT 
TGTGTGCGAC TCTTAGGTCG TAATTCGAAC TCCAAGAGGC TCTTGGGTTA TGTGGCAACT 
TTGAAGGATA ATTTTGGATT TATTGAAACA GCCAATCATG ATAAGGAAAT CTTTTTCCAT 
TACAGTGAGT TCTCTGGTGA TGTTGATAGC CTGGAACTGG GGGACATGGT CGAGTACAGC 
TTGTCCAAAG GAAAAGGCAA CAAAGTCAGT GCAGAAAAAG TGAACAAAAC ACACTCAGTG 
AATGGCATTA CTGAGGAAGC TGATCCCACC ATCTACTCTG GTAAAGTCAT TCGCCCCTTG 
AGGAGTGTTG ATCCAACACA GAATGAGTAC CAAGGAATGA TTGAGATCGT GGACGAAGGG 
GATATGAAAG GTGAGGTCTA TCCATTTGGC ATAGTTGGGA TGGCCAACAA AGGGGATTGC 
CTACAGAAAG GGGAGAGTGT CAAGTTCCAG TTGTGTGTCC TGGGCCAAAA CGCACAGACT 
ATGGCCTACA ACATCACACC CCTGCGTAGG GCTACAGTGG AGTGTGTGAA AGATCAGTTT 
GGTTTCATTA ACTATGAAGT AGGAGATAGC AAGAAGCTCT TTTTCCATGT GAAAGAAGTT 
CAGGATGGCA TTGAGCTACA AGCAGGAGAT GAGGTGGAAT TCTCAGTGAT TCTTAATCAG 
CGCACTGGCA AGTGCAGTGC TTGTAATGTT TGGCGAGTCT GCGAGGGCCC CAAGGCTGTT 
GCAGCTCCAC GACCTGATAG GCTGGTCAAT CGCTTGAAGA ATATCACCCT GGATGATGCC 
AGTGCTCCTC GCCTAATGGT TCTTCGTCAG CCAAGGGGAC CAGATAACTC AATGGGATTT 
GGTGCAGAAA GAAAGATCCG TCAAGCTGGT GTGATTGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CSDE1 ( XM_006064803.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006064865.1 Bubalus bubalis cold shock domain containing E1 (CSDE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu20443
Clone ID Related Accession (Same CDS sequence)	XM_006064803.1 , XM_006064803.2
Accession Version	XM_006064803.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cold shock domain-containing protein E1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006064803.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG TTTTTACTGT GTCATCCGAT CCTCATCCCC CTCCTGCAGC CCCTCCTTCA 
CTTTCTTTAC CTTTATCTTC ATCTTCTACC TCATCTGGGA CTAAAAAACA AAAGAGGACC 
CCCACGTATC AGAGATCTAT GAGCTTTGAT CCAAACCTTC TCCACAACAA TGGACATAAT 
GGGTACCCCA ATGGTACTTC AGCAGCACTG CGTGAGACTG GGGTTATTGA AAAACTGCTA 
ACCTCTTACG GATTTATTCA GTGTTCAGAA CGGCAAGCTA GACTTTTCTT CCACTGTTCA 
CAGTATAATG GCAACCTGCA GGACTTAAAA GTAGGAGATG ATGTTGAATT TGAAGTATCA 
TCTGATCGGC GGACTGGGAA ACCCATAGCT GTTAAACTGG TGAAGATAAA ACAAGAAATC 
CTCCCTGAAG AACGAATTAA TGGACAAGAA GTGTTTTATC TGACTTATAC CCCTGAAGAT 
GTTGAAGGGA ATGTTCAGCT GGAAACTGGA GATAAAATAA ACTTTGTAAT TGATAACAAT 
AAACATACTG GTGCTGTAAG TGCTCGTAAT ATTATGCTGT TGAAAAAGAA ACAAGCTCGC 
TATCAGGGAG TAGTTTGTGC CATGAAAGAG GCGTTTGGCT TTATTGAAAG AGGCGATATT 
GTAAAGGAGA TATTCTTTCA CTATAGTGAA TTTAAGGGTG ACTTAGAATC CTTACAGCCT 
GGAGATGACG TGGAATTCAC AATCAAGGAC CGAAATGGTA AAGAAGTTGC AACAGATGTC 
AGACTGTTGC CTCAAGGAAC AGTCATTTTT GAAGATATCA GCATTGAACA TTTTGAAGGA 
ACTGTAACCA AAGTCATCCC CAAAGTACCC AGTAAAAACC AGAATGACCC ATTGCCAGGA 
CGTATCAAAG TTGATTTTGT GATTCCTAAA GAACTTCCCT TTGGAGACAA AGATACAAAA 
TCCAAGGTGA CCCTGTTGGA AGGTGACCAC GTTAGGTTTA ATATTTCAAC AGACCGTCGT 
GACAAATTAG AACGAGCAAC CAACATAGAA GTTCTATCAA ATACATTTCA GTTCACTAAT 
GAAGCCAGAG AGATGGGTGT AATTGCTGCC ATGAGAGATG GTTTTGGTTT CATCAAGTGT 
GTGGATCGTG ATGCTCGTAT GTTCTTCCAC TTCAGTGAAA TTCTGGATGG GAACCAGCTT 
CATATTGCAG ATGAAGTAGA GTTTACTGTG GTTCCTGATA TGCTCTCTGC CCAAAGAAAT 
CATGCTATTA GGATTAAAAA ACTTCCCAAG GGCACGGTTT CGTTCCACTC CCATTCAGAT 
CATCGTTTTC TGGGCACTGT AGAAAAAGAG GCCACTTTTT CGAATCCTAA AACCACTAGC 
CCAAATAAAG GCAAAGAAAA GGAGGCTGAG GATGGCATTA TTGCTTATGA TGATTGTGGG 
GTGAAATTGA CTATTGCTTT TCAAGCCAAG GATGTGGAAG GATCTACTTC TCCTCAAATA 
GGAGACAAGG TTGAATTTAG TATTAGTGAC AAACAGAGGC CTGGACAGCA GATTGCAACT 
TGTGTGCGAC TCTTAGGTCG TAATTCGAAC TCCAAGAGGC TCTTGGGTTA TGTGGCAACT 
TTGAAGGATA ATTTTGGATT TATTGAAACA GCCAATCATG ATAAGGAAAT CTTTTTCCAT 
TACAGTGAGT TCTCTGGTGA TGTTGATAGC CTGGAACTGG GGGACATGGT CGAGTACAGC 
TTGTCCAAAG GAAAAGGCAA CAAAGTCAGT GCAGAAAAAG TGAACAAAAC ACACTCAGTG 
AATGGCATTA CTGAGGAAGC TGATCCCACC ATCTACTCTG GTAAAGTCAT TCGCCCCTTG 
AGGAGTGTTG ATCCAACACA GAATGAGTAC CAAGGAATGA TTGAGATCGT GGACGAAGGG 
GATATGAAAG GTGAGGTCTA TCCATTTGGC ATAGTTGGGA TGGCCAACAA AGGGGATTGC 
CTACAGAAAG GGGAGAGTGT CAAGTTCCAG TTGTGTGTCC TGGGCCAAAA CGCACAGACT 
ATGGCCTACA ACATCACACC CCTGCGTAGG GCTACAGTGG AGTGTGTGAA AGATCAGTTT 
GGTTTCATTA ACTATGAAGT AGGAGATAGC AAGAAGCTCT TTTTCCATGT GAAAGAAGTT 
CAGGATGGCA TTGAGCTACA AGCAGGAGAT GAGGTGGAAT TCTCAGTGAT TCTTAATCAG 
CGCACTGGCA AGTGCAGTGC TTGTAATGTT TGGCGAGTCT GCGAGGGCCC CAAGGCTGTT 
GCAGCTCCAC GACCTGATAG GCTGGTCAAT CGCTTGAAGA ATATCACCCT GGATGATGCC 
AGTGCTCCTC GCCTAATGGT TCTTCGTCAG CCAAGGGGAC CAGATAACTC AATGGGATTT 
GGTGCAGAAA GAAAGATCCG TCAAGCTGGT GTGATTGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006064865.1
CDS	550..2991
Translation	MENVFTVSSDPHPPPAAPPSLSLPLSSSSTSSGTKKQKRTPTYQRSMSFDPNLLHNNGHNGYPNGTSAALRETGVIEKLLTSYGFIQCSERQARLFFHCSQYNGNLQDLKVGDDVEFEVSSDRRTGKPIAVKLVKIKQEILPEERINGQEVFYLTYTPEDVEGNVQLETGDKINFVIDNNKHTGAVSARNIMLLKKKQARYQGVVCAMKEAFGFIERGDIVKEIFFHYSEFKGDLESLQPGDDVEFTIKDRNGKEVATDVRLLPQGTVIFEDISIEHFEGTVTKVIPKVPSKNQNDPLPGRIKVDFVIPKELPFGDKDTKSKVTLLEGDHVRFNISTDRRDKLERATNIEVLSNTFQFTNEAREMGVIAAMRDGFGFIKCVDRDARMFFHFSEILDGNQLHIADEVEFTVVPDMLSAQRNHAIRIKKLPKGTVSFHSHSDHRFLGTVEKEATFSNPKTTSPNKGKEKEAEDGIIAYDDCGVKLTIAFQAKDVEGSTSPQIGDKVEFSISDKQRPGQQIATCVRLLGRNSNSKRLLGYVATLKDNFGFIETANHDKEIFFHYSEFSGDVDSLELGDMVEYSLSKGKGNKVSAEKVNKTHSVNGITEEADPTIYSGKVIRPLRSVDPTQNEYQGMIEIVDEGDMKGEVYPFGIVGMANKGDCLQKGESVKFQLCVLGQNAQTMAYNITPLRRATVECVKDQFGFINYEVGDSKKLFFHVKEVQDGIELQAGDEVEFSVILNQRTGKCSACNVWRVCEGPKAVAAPRPDRLVNRLKNITLDDASAPRLMVLRQPRGPDNSMGFGAERKIRQAGVID

Target ORF information:

RefSeq Version	XM_006064803.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cold shock domain containing E1 (CSDE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064803.2

ORF Insert Sequence:

ATGGAGAACG TTTTTACTGT GTCATCCGAT CCTCATCCCC CTCCTGCAGC CCCTCCTTCA 
CTTTCTTTAC CTTTATCTTC ATCTTCTACC TCATCTGGGA CTAAAAAACA AAAGAGGACC 
CCCACGTATC AGAGATCTAT GAGCTTTGAT CCAAACCTTC TCCACAACAA TGGACATAAT 
GGGTACCCCA ATGGTACTTC AGCAGCACTG CGTGAGACTG GGGTTATTGA AAAACTGCTA 
ACCTCTTACG GATTTATTCA GTGTTCAGAA CGGCAAGCTA GACTTTTCTT CCACTGTTCA 
CAGTATAATG GCAACCTGCA GGACTTAAAA GTAGGAGATG ATGTTGAATT TGAAGTATCA 
TCTGATCGGC GGACTGGGAA ACCCATAGCT GTTAAACTGG TGAAGATAAA ACAAGAAATC 
CTCCCTGAAG AACGAATTAA TGGACAAGAA GTGTTTTATC TGACTTATAC CCCTGAAGAT 
GTTGAAGGGA ATGTTCAGCT GGAAACTGGA GATAAAATAA ACTTTGTAAT TGATAACAAT 
AAACATACTG GTGCTGTAAG TGCTCGTAAT ATTATGCTGT TGAAAAAGAA ACAAGCTCGC 
TATCAGGGAG TAGTTTGTGC CATGAAAGAG GCGTTTGGCT TTATTGAAAG AGGCGATATT 
GTAAAGGAGA TATTCTTTCA CTATAGTGAA TTTAAGGGTG ACTTAGAATC CTTACAGCCT 
GGAGATGACG TGGAATTCAC AATCAAGGAC CGAAATGGTA AAGAAGTTGC AACAGATGTC 
AGACTGTTGC CTCAAGGAAC AGTCATTTTT GAAGATATCA GCATTGAACA TTTTGAAGGA 
ACTGTAACCA AAGTCATCCC CAAAGTACCC AGTAAAAACC AGAATGACCC ATTGCCAGGA 
CGTATCAAAG TTGATTTTGT GATTCCTAAA GAACTTCCCT TTGGAGACAA AGATACAAAA 
TCCAAGGTGA CCCTGTTGGA AGGTGACCAC GTTAGGTTTA ATATTTCAAC AGACCGTCGT 
GACAAATTAG AACGAGCAAC CAACATAGAA GTTCTATCAA ATACATTTCA GTTCACTAAT 
GAAGCCAGAG AGATGGGTGT AATTGCTGCC ATGAGAGATG GTTTTGGTTT CATCAAGTGT 
GTGGATCGTG ATGCTCGTAT GTTCTTCCAC TTCAGTGAAA TTCTGGATGG GAACCAGCTT 
CATATTGCAG ATGAAGTAGA GTTTACTGTG GTTCCTGATA TGCTCTCTGC CCAAAGAAAT 
CATGCTATTA GGATTAAAAA ACTTCCCAAG GGCACGGTTT CGTTCCACTC CCATTCAGAT 
CATCGTTTTC TGGGCACTGT AGAAAAAGAG GCCACTTTTT CGAATCCTAA AACCACTAGC 
CCAAATAAAG GCAAAGAAAA GGAGGCTGAG GATGGCATTA TTGCTTATGA TGATTGTGGG 
GTGAAATTGA CTATTGCTTT TCAAGCCAAG GATGTGGAAG GATCTACTTC TCCTCAAATA 
GGAGACAAGG TTGAATTTAG TATTAGTGAC AAACAGAGGC CTGGACAGCA GATTGCAACT 
TGTGTGCGAC TCTTAGGTCG TAATTCGAAC TCCAAGAGGC TCTTGGGTTA TGTGGCAACT 
TTGAAGGATA ATTTTGGATT TATTGAAACA GCCAATCATG ATAAGGAAAT CTTTTTCCAT 
TACAGTGAGT TCTCTGGTGA TGTTGATAGC CTGGAACTGG GGGACATGGT CGAGTACAGC 
TTGTCCAAAG GAAAAGGCAA CAAAGTCAGT GCAGAAAAAG TGAACAAAAC ACACTCAGTG 
AATGGCATTA CTGAGGAAGC TGATCCCACC ATCTACTCTG GTAAAGTCAT TCGCCCCTTG 
AGGAGTGTTG ATCCAACACA GAATGAGTAC CAAGGAATGA TTGAGATCGT GGACGAAGGG 
GATATGAAAG GTGAGGTCTA TCCATTTGGC ATAGTTGGGA TGGCCAACAA AGGGGATTGC 
CTACAGAAAG GGGAGAGTGT CAAGTTCCAG TTGTGTGTCC TGGGCCAAAA CGCACAGACT 
ATGGCCTACA ACATCACACC CCTGCGTAGG GCTACAGTGG AGTGTGTGAA AGATCAGTTT 
GGTTTCATTA ACTATGAAGT AGGAGATAGC AAGAAGCTCT TTTTCCATGT GAAAGAAGTT 
CAGGATGGCA TTGAGCTACA AGCAGGAGAT GAGGTGGAAT TCTCAGTGAT TCTTAATCAG 
CGCACTGGCA AGTGCAGTGC TTGTAATGTT TGGCGAGTCT GCGAGGGCCC CAAGGCTGTT 
GCAGCTCCAC GACCTGATAG GCTGGTCAAT CGCTTGAAGA ATATCACCCT GGATGATGCC 
AGTGCTCCTC GCCTAATGGT TCTTCGTCAG CCAAGGGGAC CAGATAACTC AATGGGATTT 
GGTGCAGAAA GAAAGATCCG TCAAGCTGGT GTGATTGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CSDE1 ( XM_006064804.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006064866.1 Bubalus bubalis cold shock domain containing E1 (CSDE1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu20444
Clone ID Related Accession (Same CDS sequence)	XM_006064804.2 , XM_006064804.1
Accession Version	XM_006064804.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cold shock domain-containing protein E1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006064804.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTTG ATCCAAACCT TCTCCACAAC AATGGACATA ATGGGTACCC CAATGGTACT 
TCAGCAGCAC TGCGTGAGAC TGGGGTTATT GAAAAACTGC TAACCTCTTA CGGATTTATT 
CAGTGTTCAG AACGGCAAGC TAGACTTTTC TTCCACTGTT CACAGTATAA TGGCAACCTG 
CAGGACTTAA AAGTAGGAGA TGATGTTGAA TTTGAAGTAT CATCTGATCG GCGGACTGGG 
AAACCCATAG CTGTTAAACT GGTGAAGATA AAACAAGAAA TCCTCCCTGA AGAACGAATT 
AATGGACAAG TTGTGTGCGC TGTTCCTCAC AACTTAGAGA GTAAATCTCC AGCTGCCCCG 
GGTCAGAGTC CAACAGGGAG TGTATGCTAC GAACGTAATG GGGAAGTGTT TTATCTGACT 
TATACCCCTG AAGATGTTGA AGGGAATGTT CAGCTGGAAA CTGGAGATAA AATAAACTTT 
GTAATTGATA ACAATAAACA TACTGGTGCT GTAAGTGCTC GTAATATTAT GCTGTTGAAA 
AAGAAACAAG CTCGCTATCA GGGAGTAGTT TGTGCCATGA AAGAGGCGTT TGGCTTTATT 
GAAAGAGGCG ATATTGTAAA GGAGATATTC TTTCACTATA GTGAATTTAA GGGTGACTTA 
GAATCCTTAC AGCCTGGAGA TGACGTGGAA TTCACAATCA AGGACCGAAA TGGTAAAGAA 
GTTGCAACAG ATGTCAGACT GTTGCCTCAA GGAACAGTCA TTTTTGAAGA TATCAGCATT 
GAACATTTTG AAGGAACTGT AACCAAAGTC ATCCCCAAAG TACCCAGTAA AAACCAGAAT 
GACCCATTGC CAGGACGTAT CAAAGTTGAT TTTGTGATTC CTAAAGAACT TCCCTTTGGA 
GACAAAGATA CAAAATCCAA GGTGACCCTG TTGGAAGGTG ACCACGTTAG GTTTAATATT 
TCAACAGACC GTCGTGACAA ATTAGAACGA GCAACCAACA TAGAAGTTCT ATCAAATACA 
TTTCAGTTCA CTAATGAAGC CAGAGAGATG GGTGTAATTG CTGCCATGAG AGATGGTTTT 
GGTTTCATCA AGTGTGTGGA TCGTGATGCT CGTATGTTCT TCCACTTCAG TGAAATTCTG 
GATGGGAACC AGCTTCATAT TGCAGATGAA GTAGAGTTTA CTGTGGTTCC TGATATGCTC 
TCTGCCCAAA GAAATCATGC TATTAGGATT AAAAAACTTC CCAAGGGCAC GGTTTCGTTC 
CACTCCCATT CAGATCATCG TTTTCTGGGC ACTGTAGAAA AAGAGGCCAC TTTTTCGAAT 
CCTAAAACCA CTAGCCCAAA TAAAGGCAAA GAAAAGGAGG CTGAGGATGG CATTATTGCT 
TATGATGATT GTGGGGTGAA ATTGACTATT GCTTTTCAAG CCAAGGATGT GGAAGGATCT 
ACTTCTCCTC AAATAGGAGA CAAGGTTGAA TTTAGTATTA GTGACAAACA GAGGCCTGGA 
CAGCAGATTG CAACTTGTGT GCGACTCTTA GGTCGTAATT CGAACTCCAA GAGGCTCTTG 
GGTTATGTGG CAACTTTGAA GGATAATTTT GGATTTATTG AAACAGCCAA TCATGATAAG 
GAAATCTTTT TCCATTACAG TGAGTTCTCT GGTGATGTTG ATAGCCTGGA ACTGGGGGAC 
ATGGTCGAGT ACAGCTTGTC CAAAGGAAAA GGCAACAAAG TCAGTGCAGA AAAAGTGAAC 
AAAACACACT CAGTGAATGG CATTACTGAG GAAGCTGATC CCACCATCTA CTCTGGTAAA 
GTCATTCGCC CCTTGAGGAG TGTTGATCCA ACACAGAATG AGTACCAAGG AATGATTGAG 
ATCGTGGACG AAGGGGATAT GAAAGGTGAG GTCTATCCAT TTGGCATAGT TGGGATGGCC 
AACAAAGGGG ATTGCCTACA GAAAGGGGAG AGTGTCAAGT TCCAGTTGTG TGTCCTGGGC 
CAAAACGCAC AGACTATGGC CTACAACATC ACACCCCTGC GTAGGGCTAC AGTGGAGTGT 
GTGAAAGATC AGTTTGGTTT CATTAACTAT GAAGTAGGAG ATAGCAAGAA GCTCTTTTTC 
CATGTGAAAG AAGTTCAGGA TGGCATTGAG CTACAAGCAG GAGATGAGGT GGAATTCTCA 
GTGATTCTTA ATCAGCGCAC TGGCAAGTGC AGTGCTTGTA ATGTTTGGCG AGTCTGCGAG 
GGCCCCAAGG CTGTTGCAGC TCCACGACCT GATAGGCTGG TCAATCGCTT GAAGAATATC 
ACCCTGGATG ATGCCAGTGC TCCTCGCCTA ATGGTTCTTC GTCAGCCAAG GGGACCAGAT 
AACTCAATGG GATTTGGTGC AGAAAGAAAG ATCCGTCAAG CTGGTGTGAT TGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006064866.1
CDS	541..2937
Translation	MSFDPNLLHNNGHNGYPNGTSAALRETGVIEKLLTSYGFIQCSERQARLFFHCSQYNGNLQDLKVGDDVEFEVSSDRRTGKPIAVKLVKIKQEILPEERINGQVVCAVPHNLESKSPAAPGQSPTGSVCYERNGEVFYLTYTPEDVEGNVQLETGDKINFVIDNNKHTGAVSARNIMLLKKKQARYQGVVCAMKEAFGFIERGDIVKEIFFHYSEFKGDLESLQPGDDVEFTIKDRNGKEVATDVRLLPQGTVIFEDISIEHFEGTVTKVIPKVPSKNQNDPLPGRIKVDFVIPKELPFGDKDTKSKVTLLEGDHVRFNISTDRRDKLERATNIEVLSNTFQFTNEAREMGVIAAMRDGFGFIKCVDRDARMFFHFSEILDGNQLHIADEVEFTVVPDMLSAQRNHAIRIKKLPKGTVSFHSHSDHRFLGTVEKEATFSNPKTTSPNKGKEKEAEDGIIAYDDCGVKLTIAFQAKDVEGSTSPQIGDKVEFSISDKQRPGQQIATCVRLLGRNSNSKRLLGYVATLKDNFGFIETANHDKEIFFHYSEFSGDVDSLELGDMVEYSLSKGKGNKVSAEKVNKTHSVNGITEEADPTIYSGKVIRPLRSVDPTQNEYQGMIEIVDEGDMKGEVYPFGIVGMANKGDCLQKGESVKFQLCVLGQNAQTMAYNITPLRRATVECVKDQFGFINYEVGDSKKLFFHVKEVQDGIELQAGDEVEFSVILNQRTGKCSACNVWRVCEGPKAVAAPRPDRLVNRLKNITLDDASAPRLMVLRQPRGPDNSMGFGAERKIRQAGVID

Target ORF information:

RefSeq Version	XM_006064804.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cold shock domain containing E1 (CSDE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064804.2

ORF Insert Sequence:

ATGAGCTTTG ATCCAAACCT TCTCCACAAC AATGGACATA ATGGGTACCC CAATGGTACT 
TCAGCAGCAC TGCGTGAGAC TGGGGTTATT GAAAAACTGC TAACCTCTTA CGGATTTATT 
CAGTGTTCAG AACGGCAAGC TAGACTTTTC TTCCACTGTT CACAGTATAA TGGCAACCTG 
CAGGACTTAA AAGTAGGAGA TGATGTTGAA TTTGAAGTAT CATCTGATCG GCGGACTGGG 
AAACCCATAG CTGTTAAACT GGTGAAGATA AAACAAGAAA TCCTCCCTGA AGAACGAATT 
AATGGACAAG TTGTGTGCGC TGTTCCTCAC AACTTAGAGA GTAAATCTCC AGCTGCCCCG 
GGTCAGAGTC CAACAGGGAG TGTATGCTAC GAACGTAATG GGGAAGTGTT TTATCTGACT 
TATACCCCTG AAGATGTTGA AGGGAATGTT CAGCTGGAAA CTGGAGATAA AATAAACTTT 
GTAATTGATA ACAATAAACA TACTGGTGCT GTAAGTGCTC GTAATATTAT GCTGTTGAAA 
AAGAAACAAG CTCGCTATCA GGGAGTAGTT TGTGCCATGA AAGAGGCGTT TGGCTTTATT 
GAAAGAGGCG ATATTGTAAA GGAGATATTC TTTCACTATA GTGAATTTAA GGGTGACTTA 
GAATCCTTAC AGCCTGGAGA TGACGTGGAA TTCACAATCA AGGACCGAAA TGGTAAAGAA 
GTTGCAACAG ATGTCAGACT GTTGCCTCAA GGAACAGTCA TTTTTGAAGA TATCAGCATT 
GAACATTTTG AAGGAACTGT AACCAAAGTC ATCCCCAAAG TACCCAGTAA AAACCAGAAT 
GACCCATTGC CAGGACGTAT CAAAGTTGAT TTTGTGATTC CTAAAGAACT TCCCTTTGGA 
GACAAAGATA CAAAATCCAA GGTGACCCTG TTGGAAGGTG ACCACGTTAG GTTTAATATT 
TCAACAGACC GTCGTGACAA ATTAGAACGA GCAACCAACA TAGAAGTTCT ATCAAATACA 
TTTCAGTTCA CTAATGAAGC CAGAGAGATG GGTGTAATTG CTGCCATGAG AGATGGTTTT 
GGTTTCATCA AGTGTGTGGA TCGTGATGCT CGTATGTTCT TCCACTTCAG TGAAATTCTG 
GATGGGAACC AGCTTCATAT TGCAGATGAA GTAGAGTTTA CTGTGGTTCC TGATATGCTC 
TCTGCCCAAA GAAATCATGC TATTAGGATT AAAAAACTTC CCAAGGGCAC GGTTTCGTTC 
CACTCCCATT CAGATCATCG TTTTCTGGGC ACTGTAGAAA AAGAGGCCAC TTTTTCGAAT 
CCTAAAACCA CTAGCCCAAA TAAAGGCAAA GAAAAGGAGG CTGAGGATGG CATTATTGCT 
TATGATGATT GTGGGGTGAA ATTGACTATT GCTTTTCAAG CCAAGGATGT GGAAGGATCT 
ACTTCTCCTC AAATAGGAGA CAAGGTTGAA TTTAGTATTA GTGACAAACA GAGGCCTGGA 
CAGCAGATTG CAACTTGTGT GCGACTCTTA GGTCGTAATT CGAACTCCAA GAGGCTCTTG 
GGTTATGTGG CAACTTTGAA GGATAATTTT GGATTTATTG AAACAGCCAA TCATGATAAG 
GAAATCTTTT TCCATTACAG TGAGTTCTCT GGTGATGTTG ATAGCCTGGA ACTGGGGGAC 
ATGGTCGAGT ACAGCTTGTC CAAAGGAAAA GGCAACAAAG TCAGTGCAGA AAAAGTGAAC 
AAAACACACT CAGTGAATGG CATTACTGAG GAAGCTGATC CCACCATCTA CTCTGGTAAA 
GTCATTCGCC CCTTGAGGAG TGTTGATCCA ACACAGAATG AGTACCAAGG AATGATTGAG 
ATCGTGGACG AAGGGGATAT GAAAGGTGAG GTCTATCCAT TTGGCATAGT TGGGATGGCC 
AACAAAGGGG ATTGCCTACA GAAAGGGGAG AGTGTCAAGT TCCAGTTGTG TGTCCTGGGC 
CAAAACGCAC AGACTATGGC CTACAACATC ACACCCCTGC GTAGGGCTAC AGTGGAGTGT 
GTGAAAGATC AGTTTGGTTT CATTAACTAT GAAGTAGGAG ATAGCAAGAA GCTCTTTTTC 
CATGTGAAAG AAGTTCAGGA TGGCATTGAG CTACAAGCAG GAGATGAGGT GGAATTCTCA 
GTGATTCTTA ATCAGCGCAC TGGCAAGTGC AGTGCTTGTA ATGTTTGGCG AGTCTGCGAG 
GGCCCCAAGG CTGTTGCAGC TCCACGACCT GATAGGCTGG TCAATCGCTT GAAGAATATC 
ACCCTGGATG ATGCCAGTGC TCCTCGCCTA ATGGTTCTTC GTCAGCCAAG GGGACCAGAT 
AACTCAATGG GATTTGGTGC AGAAAGAAAG ATCCGTCAAG CTGGTGTGAT TGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CSDE1 ( XM_006064804.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006064866.1 Bubalus bubalis cold shock domain containing E1, RNA-binding (CSDE1), transcript variant X3, mRNA.

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CloneID	OBu20444
Clone ID Related Accession (Same CDS sequence)	XM_006064804.2 , XM_006064804.1
Accession Version	XM_006064804.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	cold shock domain-containing protein E1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785149.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTTG ATCCAAACCT TCTCCACAAC AATGGACATA ATGGGTACCC CAATGGTACT 
TCAGCAGCAC TGCGTGAGAC TGGGGTTATT GAAAAACTGC TAACCTCTTA CGGATTTATT 
CAGTGTTCAG AACGGCAAGC TAGACTTTTC TTCCACTGTT CACAGTATAA TGGCAACCTG 
CAGGACTTAA AAGTAGGAGA TGATGTTGAA TTTGAAGTAT CATCTGATCG GCGGACTGGG 
AAACCCATAG CTGTTAAACT GGTGAAGATA AAACAAGAAA TCCTCCCTGA AGAACGAATT 
AATGGACAAG TTGTGTGCGC TGTTCCTCAC AACTTAGAGA GTAAATCTCC AGCTGCCCCG 
GGTCAGAGTC CAACAGGGAG TGTATGCTAC GAACGTAATG GGGAAGTGTT TTATCTGACT 
TATACCCCTG AAGATGTTGA AGGGAATGTT CAGCTGGAAA CTGGAGATAA AATAAACTTT 
GTAATTGATA ACAATAAACA TACTGGTGCT GTAAGTGCTC GTAATATTAT GCTGTTGAAA 
AAGAAACAAG CTCGCTATCA GGGAGTAGTT TGTGCCATGA AAGAGGCGTT TGGCTTTATT 
GAAAGAGGCG ATATTGTAAA GGAGATATTC TTTCACTATA GTGAATTTAA GGGTGACTTA 
GAATCCTTAC AGCCTGGAGA TGACGTGGAA TTCACAATCA AGGACCGAAA TGGTAAAGAA 
GTTGCAACAG ATGTCAGACT GTTGCCTCAA GGAACAGTCA TTTTTGAAGA TATCAGCATT 
GAACATTTTG AAGGAACTGT AACCAAAGTC ATCCCCAAAG TACCCAGTAA AAACCAGAAT 
GACCCATTGC CAGGACGTAT CAAAGTTGAT TTTGTGATTC CTAAAGAACT TCCCTTTGGA 
GACAAAGATA CAAAATCCAA GGTGACCCTG TTGGAAGGTG ACCACGTTAG GTTTAATATT 
TCAACAGACC GTCGTGACAA ATTAGAACGA GCAACCAACA TAGAAGTTCT ATCAAATACA 
TTTCAGTTCA CTAATGAAGC CAGAGAGATG GGTGTAATTG CTGCCATGAG AGATGGTTTT 
GGTTTCATCA AGTGTGTGGA TCGTGATGCT CGTATGTTCT TCCACTTCAG TGAAATTCTG 
GATGGGAACC AGCTTCATAT TGCAGATGAA GTAGAGTTTA CTGTGGTTCC TGATATGCTC 
TCTGCCCAAA GAAATCATGC TATTAGGATT AAAAAACTTC CCAAGGGCAC GGTTTCGTTC 
CACTCCCATT CAGATCATCG TTTTCTGGGC ACTGTAGAAA AAGAGGCCAC TTTTTCGAAT 
CCTAAAACCA CTAGCCCAAA TAAAGGCAAA GAAAAGGAGG CTGAGGATGG CATTATTGCT 
TATGATGATT GTGGGGTGAA ATTGACTATT GCTTTTCAAG CCAAGGATGT GGAAGGATCT 
ACTTCTCCTC AAATAGGAGA CAAGGTTGAA TTTAGTATTA GTGACAAACA GAGGCCTGGA 
CAGCAGATTG CAACTTGTGT GCGACTCTTA GGTCGTAATT CGAACTCCAA GAGGCTCTTG 
GGTTATGTGG CAACTTTGAA GGATAATTTT GGATTTATTG AAACAGCCAA TCATGATAAG 
GAAATCTTTT TCCATTACAG TGAGTTCTCT GGTGATGTTG ATAGCCTGGA ACTGGGGGAC 
ATGGTCGAGT ACAGCTTGTC CAAAGGAAAA GGCAACAAAG TCAGTGCAGA AAAAGTGAAC 
AAAACACACT CAGTGAATGG CATTACTGAG GAAGCTGATC CCACCATCTA CTCTGGTAAA 
GTCATTCGCC CCTTGAGGAG TGTTGATCCA ACACAGAATG AGTACCAAGG AATGATTGAG 
ATCGTGGACG AAGGGGATAT GAAAGGTGAG GTCTATCCAT TTGGCATAGT TGGGATGGCC 
AACAAAGGGG ATTGCCTACA GAAAGGGGAG AGTGTCAAGT TCCAGTTGTG TGTCCTGGGC 
CAAAACGCAC AGACTATGGC CTACAACATC ACACCCCTGC GTAGGGCTAC AGTGGAGTGT 
GTGAAAGATC AGTTTGGTTT CATTAACTAT GAAGTAGGAG ATAGCAAGAA GCTCTTTTTC 
CATGTGAAAG AAGTTCAGGA TGGCATTGAG CTACAAGCAG GAGATGAGGT GGAATTCTCA 
GTGATTCTTA ATCAGCGCAC TGGCAAGTGC AGTGCTTGTA ATGTTTGGCG AGTCTGCGAG 
GGCCCCAAGG CTGTTGCAGC TCCACGACCT GATAGGCTGG TCAATCGCTT GAAGAATATC 
ACCCTGGATG ATGCCAGTGC TCCTCGCCTA ATGGTTCTTC GTCAGCCAAG GGGACCAGAT 
AACTCAATGG GATTTGGTGC AGAAAGAAAG ATCCGTCAAG CTGGTGTGAT TGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006064866.1
CDS	505..2901
Translation	MSFDPNLLHNNGHNGYPNGTSAALRETGVIEKLLTSYGFIQCSERQARLFFHCSQYNGNLQDLKVGDDVEFEVSSDRRTGKPIAVKLVKIKQEILPEERINGQVVCAVPHNLESKSPAAPGQSPTGSVCYERNGEVFYLTYTPEDVEGNVQLETGDKINFVIDNNKHTGAVSARNIMLLKKKQARYQGVVCAMKEAFGFIERGDIVKEIFFHYSEFKGDLESLQPGDDVEFTIKDRNGKEVATDVRLLPQGTVIFEDISIEHFEGTVTKVIPKVPSKNQNDPLPGRIKVDFVIPKELPFGDKDTKSKVTLLEGDHVRFNISTDRRDKLERATNIEVLSNTFQFTNEAREMGVIAAMRDGFGFIKCVDRDARMFFHFSEILDGNQLHIADEVEFTVVPDMLSAQRNHAIRIKKLPKGTVSFHSHSDHRFLGTVEKEATFSNPKTTSPNKGKEKEAEDGIIAYDDCGVKLTIAFQAKDVEGSTSPQIGDKVEFSISDKQRPGQQIATCVRLLGRNSNSKRLLGYVATLKDNFGFIETANHDKEIFFHYSEFSGDVDSLELGDMVEYSLSKGKGNKVSAEKVNKTHSVNGITEEADPTIYSGKVIRPLRSVDPTQNEYQGMIEIVDEGDMKGEVYPFGIVGMANKGDCLQKGESVKFQLCVLGQNAQTMAYNITPLRRATVECVKDQFGFINYEVGDSKKLFFHVKEVQDGIELQAGDEVEFSVILNQRTGKCSACNVWRVCEGPKAVAAPRPDRLVNRLKNITLDDASAPRLMVLRQPRGPDNSMGFGAERKIRQAGVID

Target ORF information:

RefSeq Version	XM_006064804.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cold shock domain containing E1, RNA-binding (CSDE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064804.1

ORF Insert Sequence:

ATGAGCTTTG ATCCAAACCT TCTCCACAAC AATGGACATA ATGGGTACCC CAATGGTACT 
TCAGCAGCAC TGCGTGAGAC TGGGGTTATT GAAAAACTGC TAACCTCTTA CGGATTTATT 
CAGTGTTCAG AACGGCAAGC TAGACTTTTC TTCCACTGTT CACAGTATAA TGGCAACCTG 
CAGGACTTAA AAGTAGGAGA TGATGTTGAA TTTGAAGTAT CATCTGATCG GCGGACTGGG 
AAACCCATAG CTGTTAAACT GGTGAAGATA AAACAAGAAA TCCTCCCTGA AGAACGAATT 
AATGGACAAG TTGTGTGCGC TGTTCCTCAC AACTTAGAGA GTAAATCTCC AGCTGCCCCG 
GGTCAGAGTC CAACAGGGAG TGTATGCTAC GAACGTAATG GGGAAGTGTT TTATCTGACT 
TATACCCCTG AAGATGTTGA AGGGAATGTT CAGCTGGAAA CTGGAGATAA AATAAACTTT 
GTAATTGATA ACAATAAACA TACTGGTGCT GTAAGTGCTC GTAATATTAT GCTGTTGAAA 
AAGAAACAAG CTCGCTATCA GGGAGTAGTT TGTGCCATGA AAGAGGCGTT TGGCTTTATT 
GAAAGAGGCG ATATTGTAAA GGAGATATTC TTTCACTATA GTGAATTTAA GGGTGACTTA 
GAATCCTTAC AGCCTGGAGA TGACGTGGAA TTCACAATCA AGGACCGAAA TGGTAAAGAA 
GTTGCAACAG ATGTCAGACT GTTGCCTCAA GGAACAGTCA TTTTTGAAGA TATCAGCATT 
GAACATTTTG AAGGAACTGT AACCAAAGTC ATCCCCAAAG TACCCAGTAA AAACCAGAAT 
GACCCATTGC CAGGACGTAT CAAAGTTGAT TTTGTGATTC CTAAAGAACT TCCCTTTGGA 
GACAAAGATA CAAAATCCAA GGTGACCCTG TTGGAAGGTG ACCACGTTAG GTTTAATATT 
TCAACAGACC GTCGTGACAA ATTAGAACGA GCAACCAACA TAGAAGTTCT ATCAAATACA 
TTTCAGTTCA CTAATGAAGC CAGAGAGATG GGTGTAATTG CTGCCATGAG AGATGGTTTT 
GGTTTCATCA AGTGTGTGGA TCGTGATGCT CGTATGTTCT TCCACTTCAG TGAAATTCTG 
GATGGGAACC AGCTTCATAT TGCAGATGAA GTAGAGTTTA CTGTGGTTCC TGATATGCTC 
TCTGCCCAAA GAAATCATGC TATTAGGATT AAAAAACTTC CCAAGGGCAC GGTTTCGTTC 
CACTCCCATT CAGATCATCG TTTTCTGGGC ACTGTAGAAA AAGAGGCCAC TTTTTCGAAT 
CCTAAAACCA CTAGCCCAAA TAAAGGCAAA GAAAAGGAGG CTGAGGATGG CATTATTGCT 
TATGATGATT GTGGGGTGAA ATTGACTATT GCTTTTCAAG CCAAGGATGT GGAAGGATCT 
ACTTCTCCTC AAATAGGAGA CAAGGTTGAA TTTAGTATTA GTGACAAACA GAGGCCTGGA 
CAGCAGATTG CAACTTGTGT GCGACTCTTA GGTCGTAATT CGAACTCCAA GAGGCTCTTG 
GGTTATGTGG CAACTTTGAA GGATAATTTT GGATTTATTG AAACAGCCAA TCATGATAAG 
GAAATCTTTT TCCATTACAG TGAGTTCTCT GGTGATGTTG ATAGCCTGGA ACTGGGGGAC 
ATGGTCGAGT ACAGCTTGTC CAAAGGAAAA GGCAACAAAG TCAGTGCAGA AAAAGTGAAC 
AAAACACACT CAGTGAATGG CATTACTGAG GAAGCTGATC CCACCATCTA CTCTGGTAAA 
GTCATTCGCC CCTTGAGGAG TGTTGATCCA ACACAGAATG AGTACCAAGG AATGATTGAG 
ATCGTGGACG AAGGGGATAT GAAAGGTGAG GTCTATCCAT TTGGCATAGT TGGGATGGCC 
AACAAAGGGG ATTGCCTACA GAAAGGGGAG AGTGTCAAGT TCCAGTTGTG TGTCCTGGGC 
CAAAACGCAC AGACTATGGC CTACAACATC ACACCCCTGC GTAGGGCTAC AGTGGAGTGT 
GTGAAAGATC AGTTTGGTTT CATTAACTAT GAAGTAGGAG ATAGCAAGAA GCTCTTTTTC 
CATGTGAAAG AAGTTCAGGA TGGCATTGAG CTACAAGCAG GAGATGAGGT GGAATTCTCA 
GTGATTCTTA ATCAGCGCAC TGGCAAGTGC AGTGCTTGTA ATGTTTGGCG AGTCTGCGAG 
GGCCCCAAGG CTGTTGCAGC TCCACGACCT GATAGGCTGG TCAATCGCTT GAAGAATATC 
ACCCTGGATG ATGCCAGTGC TCCTCGCCTA ATGGTTCTTC GTCAGCCAAG GGGACCAGAT 
AACTCAATGG GATTTGGTGC AGAAAGAAAG ATCCGTCAAG CTGGTGTGAT TGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CSDE1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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