CDC42BPA gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006062435.1	XP_006062497.1	serine/threonine-protein kinase MRCK alpha isoform X10
XM_006062437.1	XP_006062499.1	serine/threonine-protein kinase MRCK alpha isoform X12
XM_006062439.1	XP_006062501.1	serine/threonine-protein kinase MRCK alpha isoform X14
XM_006062428.1	XP_006062490.1	serine/threonine-protein kinase MRCK alpha isoform X3
XM_006062427.2	XP_006062489.1	serine/threonine-protein kinase MRCK alpha isoform X2
XM_006062436.2	XP_006062498.1	serine/threonine-protein kinase MRCK alpha isoform X11
XM_006062439.2	XP_006062501.1	serine/threonine-protein kinase MRCK alpha isoform X13
XM_025286026.1	XP_025141811.1	serine/threonine-protein kinase MRCK alpha isoform X14
XM_006062430.1	XP_006062492.1	serine/threonine-protein kinase MRCK alpha isoform X5
XM_006062442.1	XP_006062504.1	serine/threonine-protein kinase MRCK alpha isoform X17
XM_006062434.2	XP_006062496.1	serine/threonine-protein kinase MRCK alpha isoform X8
XM_006062440.2	XP_006062502.1	serine/threonine-protein kinase MRCK alpha isoform X15
XM_006062431.1	XP_006062493.1	serine/threonine-protein kinase MRCK alpha isoform X6
XM_006062434.1	XP_006062496.1	serine/threonine-protein kinase MRCK alpha isoform X9
XM_006062427.1	XP_006062489.1	serine/threonine-protein kinase MRCK alpha isoform X2
XM_006062426.2	XP_006062488.1	serine/threonine-protein kinase MRCK alpha isoform X1
XM_006062429.2	XP_006062491.1	serine/threonine-protein kinase MRCK alpha isoform X4
XM_025286024.1	XP_025141809.1	serine/threonine-protein kinase MRCK alpha isoform X5
XM_006062431.2	XP_006062493.1	serine/threonine-protein kinase MRCK alpha isoform X6
XM_006062433.2	XP_006062495.1	serine/threonine-protein kinase MRCK alpha isoform X7
XM_006062435.2	XP_006062497.1	serine/threonine-protein kinase MRCK alpha isoform X9
XM_025286025.1	XP_025141810.1	serine/threonine-protein kinase MRCK alpha isoform X10
XM_006062432.1	XP_006062494.1	serine/threonine-protein kinase MRCK alpha isoform X7
XM_006062433.1	XP_006062495.1	serine/threonine-protein kinase MRCK alpha isoform X8
XM_006062436.1	XP_006062498.1	serine/threonine-protein kinase MRCK alpha isoform X11
XM_006062438.1	XP_006062500.1	serine/threonine-protein kinase MRCK alpha isoform X13
XM_006062426.1	XP_006062488.1	serine/threonine-protein kinase MRCK alpha isoform X1
XM_006062428.2	XP_006062490.1	serine/threonine-protein kinase MRCK alpha isoform X3
XM_006062440.1	XP_006062502.1	serine/threonine-protein kinase MRCK alpha isoform X15
XM_006062429.1	XP_006062491.1	serine/threonine-protein kinase MRCK alpha isoform X4
XM_006062441.1	XP_006062503.1	serine/threonine-protein kinase MRCK alpha isoform X16
XM_006062438.2	XP_006062500.1	serine/threonine-protein kinase MRCK alpha isoform X12
XM_006062442.2	XP_006062504.1	serine/threonine-protein kinase MRCK alpha isoform X16

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu18503	XM_006062426.2 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18504	XM_006062427.2 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18505	XM_006062428.2 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18506	XM_006062429.2 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18507	XM_006062430.1 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18508	XM_006062431.2 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18509	XM_006062432.1 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18510	XM_006062433.2 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18511	XM_006062434.2 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18512	XM_006062435.2 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18513	XM_006062436.2 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18514	XM_006062437.1 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18515	XM_006062438.2 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18516	XM_006062439.2 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18517	XM_006062440.2 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18518	XM_006062441.1 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18519	XM_006062442.2 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu66401	XM_025286024.1 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu66402	XM_025286025.1 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu66403	XM_025286026.1 Latest version!	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OBu18516	XM_006062439.1	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18511	XM_006062434.1	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18504	XM_006062427.1	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18508	XM_006062431.1	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18512	XM_006062435.1	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18510	XM_006062433.1	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18513	XM_006062436.1	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18503	XM_006062426.1	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18505	XM_006062428.1	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18517	XM_006062440.1	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18506	XM_006062429.1	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18515	XM_006062438.1	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBu18519	XM_006062442.1	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

CDC42BPA ( XM_006062426.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062488.1 Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu18503
Clone ID Related Accession (Same CDS sequence)	XM_006062426.2 , XM_006062426.1
Accession Version	XM_006062426.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006062426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGATCCC 
GTTGAGAACA CATATGTACG GAATCCGAAC GTCAAGTTTT ACATCCAGTC AAGGTCCACC 
TCTCCTTCCA CATCTAGTGA AGCTGAGCCA GTTAAGACTG TAGACTCTAC TCCACTTCCA 
GTTCATACAC CAACCTTAAG GAAAAAGGGA TGCCCTGGCT CAACTGGCTT TCCATCGAAG 
CGCAAGATTC ATCAGTTTTT TGTCAAATCT TTTACTACTC CCACCAGGTG TCATCAGTGT 
ACATCTTTGA TGGTGGGTTT GATAAGACAA GGCTGTTCTT GTGAAGTGTG TGGATTTTCA 
TGTCACATAA CTTGTGTAAA CAAAGCTCCA ACTGCTTGTC CAGTTCCTCC TGAACAGACA 
AAAGGTCCTC TGGGTATAGA TCCACAGAAA GGAATAGGAA CAGCATATGA AGGGCATGTC 
AGGATCCCTA AGCCAGCTGG AGTGAAGAAA GGATGGCAAA GAGCACTGGC TGTAGTCTGT 
GACTTCAAGC TCTTTCTGTA CGATATTGCC GAAGGAAAGG CATCTCAGCC CAGTGTTGTA 
GTCAGTCAAG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT CTTGGCTTCT 
GATGTTATTC ATGCAAGTCG AAAAGATATA CCCTGTATAT TTAGAGTCAC AGCTTCCCAG 
CTCTCAGCAT CTAGTAACAA ATGTTCCATC CTGATGCTAG CTGATAGTGA AAATGAGAAG 
AGTAAATGGG TGGGAGTACT GAGTGAATTG CACAAGATTT TGAAGAAAAA CAAATTCAGA 
GACCGCTCAG TCTATGTTCC CAAAGAGGCT TATGACAGCA CTCTGCCCCT CATTAAAACA 
ACCCAGGCAG CTGCAATCAT AGATCATGAA AGAATAGCTT TGGGGAATGA AGAAGGGTTA 
TTTGTTGTGC ATGTCACCAA AGATGAAATT ATTAGAGTTG GTGACAATAA GAAGATTCAT 
CAGATAGAAC TCATTCCAAA TGATCAACTT GTTGCCGTGA TCTCAGGACG AAATCGACAC 
GTCCGACTTT TTCCTATGTC AGCTCTGGAC GGGCGAGAGA CTGATTTTTA TAAGCTGGCA 
GAAACTAAAG GCTGTCAAAC AATAACTTCT GGCAAAGTGC GTCATGGAGC TCTTACATGC 
TTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACGCGT 
CACAGAAAAT TTAAGGAAAT TCAAGTCCCG TACAACGTCC AGTGGATGGC GATCTTCAGT 
GAGCAGCTCT GTGTGGGATT CCAGTCTGGA TTTCTAAGAT ATCCCCTGAA TGGAGAAGGA 
AGTCCATACA GTATGCTCCA TTCAAATGAC AATACACTCT CATTTATTGC ACATCAACCA 
ATGGATGCCA TCTGTGCGGT TGAAATCTCC AGTAAAGAAT ACCTGCTGTG TTTTAACAGC 
ATTGGAATAT ACACTGACTG CCAGGGCCGA AGATCTAGAC AACAGGAACT GATGTGGCCA 
GCGAATCCTT CCTCTTGTTG TTACAATGCA CCGTATCTCT CAGTGTACAG TGAAAACGCA 
GTTGACATCT TTGATGTGAA CTCCATGGAA TGGATTCAGA CTCTCCCACT CAAAAAGGTT 
CGCCCCTTAA ACAGTGAAGG ATCATTAAAT CTTTTAGGCT TGGAGACGAT TAGATTGATA 
TATTTCAAAA ATAAAATGGC AGAAGGGGAT GAACTGGTAG TTCCTGAAAC ATCGGATAAT 
AGCCGGAAGC AAATGGTTAG AAATATTAAC AATAAGAGGC GTTACTCTTT CAGAGTTCCG 
GAAGAGGAAA GAATGCAACA GAGGAGGGAG ATGTTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACATA TGGGTCCAGG AGATGGAATA 
CAGATCCTAA AAGACCTGCC CATGCCGGGT TTCCCTTATC CATCCCCTCA TCATCACTCC 
GGTCTGATCT CCTCTCCGAT CAACTTTGAG CACATCTATC ACATGACTGT TAATTCTGCT 
GAAAAATTTC TCTCTCCTGA TTCCATAAAC CCTGAATATT CCCCGTCTCT GCGCTCTGTC 
CCTGGTACAC CAAGCTTTAT GACTCTGAGG AACCCTCGGC CTCAGGAAAG TCGGACAGCA 
TTCAGTGGCT CGGTCAGTAT TCCGTCTATC ACCAAATCCC GCCCTGAACC CGGTCGCTCC 
ATGAGCGCCA GCAGTGGCCT ATCTGCAAGG TCATCTGCAC AGAATGGCAG TGCGTTAAAG 
AGAGAATTCT CTGGAGGCAG CTACAGTGCA AAGCGGCAGC CCATGCCCTC CCCATCGGAG 
GGCTCGCTGT CCTCAGGAGG CATGGACCAG GGAAGTGACG CCCCAGCGCG GGACTATGAC 
GGAGAGGACT CTGATTCTCC GCGGCATTCC ACGGCTTCCA ACAGTTCCAA CCTGAGCAGT 
CCTCCCAGCC CGGTGTCGCC CCGGAAGACC AAGAGCCTGT CTCTGGAGAG CACAGACCGC 
GGGAGCTGGG ACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062488.1
CDS	766..6303
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062426.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062426.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGATCCC 
GTTGAGAACA CATATGTACG GAATCCGAAC GTCAAGTTTT ACATCCAGTC AAGGTCCACC 
TCTCCTTCCA CATCTAGTGA AGCTGAGCCA GTTAAGACTG TAGACTCTAC TCCACTTCCA 
GTTCATACAC CAACCTTAAG GAAAAAGGGA TGCCCTGGCT CAACTGGCTT TCCATCGAAG 
CGCAAGATTC ATCAGTTTTT TGTCAAATCT TTTACTACTC CCACCAGGTG TCATCAGTGT 
ACATCTTTGA TGGTGGGTTT GATAAGACAA GGCTGTTCTT GTGAAGTGTG TGGATTTTCA 
TGTCACATAA CTTGTGTAAA CAAAGCTCCA ACTGCTTGTC CAGTTCCTCC TGAACAGACA 
AAAGGTCCTC TGGGTATAGA TCCACAGAAA GGAATAGGAA CAGCATATGA AGGGCATGTC 
AGGATCCCTA AGCCAGCTGG AGTGAAGAAA GGATGGCAAA GAGCACTGGC TGTAGTCTGT 
GACTTCAAGC TCTTTCTGTA CGATATTGCC GAAGGAAAGG CATCTCAGCC CAGTGTTGTA 
GTCAGTCAAG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT CTTGGCTTCT 
GATGTTATTC ATGCAAGTCG AAAAGATATA CCCTGTATAT TTAGAGTCAC AGCTTCCCAG 
CTCTCAGCAT CTAGTAACAA ATGTTCCATC CTGATGCTAG CTGATAGTGA AAATGAGAAG 
AGTAAATGGG TGGGAGTACT GAGTGAATTG CACAAGATTT TGAAGAAAAA CAAATTCAGA 
GACCGCTCAG TCTATGTTCC CAAAGAGGCT TATGACAGCA CTCTGCCCCT CATTAAAACA 
ACCCAGGCAG CTGCAATCAT AGATCATGAA AGAATAGCTT TGGGGAATGA AGAAGGGTTA 
TTTGTTGTGC ATGTCACCAA AGATGAAATT ATTAGAGTTG GTGACAATAA GAAGATTCAT 
CAGATAGAAC TCATTCCAAA TGATCAACTT GTTGCCGTGA TCTCAGGACG AAATCGACAC 
GTCCGACTTT TTCCTATGTC AGCTCTGGAC GGGCGAGAGA CTGATTTTTA TAAGCTGGCA 
GAAACTAAAG GCTGTCAAAC AATAACTTCT GGCAAAGTGC GTCATGGAGC TCTTACATGC 
TTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACGCGT 
CACAGAAAAT TTAAGGAAAT TCAAGTCCCG TACAACGTCC AGTGGATGGC GATCTTCAGT 
GAGCAGCTCT GTGTGGGATT CCAGTCTGGA TTTCTAAGAT ATCCCCTGAA TGGAGAAGGA 
AGTCCATACA GTATGCTCCA TTCAAATGAC AATACACTCT CATTTATTGC ACATCAACCA 
ATGGATGCCA TCTGTGCGGT TGAAATCTCC AGTAAAGAAT ACCTGCTGTG TTTTAACAGC 
ATTGGAATAT ACACTGACTG CCAGGGCCGA AGATCTAGAC AACAGGAACT GATGTGGCCA 
GCGAATCCTT CCTCTTGTTG TTACAATGCA CCGTATCTCT CAGTGTACAG TGAAAACGCA 
GTTGACATCT TTGATGTGAA CTCCATGGAA TGGATTCAGA CTCTCCCACT CAAAAAGGTT 
CGCCCCTTAA ACAGTGAAGG ATCATTAAAT CTTTTAGGCT TGGAGACGAT TAGATTGATA 
TATTTCAAAA ATAAAATGGC AGAAGGGGAT GAACTGGTAG TTCCTGAAAC ATCGGATAAT 
AGCCGGAAGC AAATGGTTAG AAATATTAAC AATAAGAGGC GTTACTCTTT CAGAGTTCCG 
GAAGAGGAAA GAATGCAACA GAGGAGGGAG ATGTTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACATA TGGGTCCAGG AGATGGAATA 
CAGATCCTAA AAGACCTGCC CATGCCGGGT TTCCCTTATC CATCCCCTCA TCATCACTCC 
GGTCTGATCT CCTCTCCGAT CAACTTTGAG CACATCTATC ACATGACTGT TAATTCTGCT 
GAAAAATTTC TCTCTCCTGA TTCCATAAAC CCTGAATATT CCCCGTCTCT GCGCTCTGTC 
CCTGGTACAC CAAGCTTTAT GACTCTGAGG AACCCTCGGC CTCAGGAAAG TCGGACAGCA 
TTCAGTGGCT CGGTCAGTAT TCCGTCTATC ACCAAATCCC GCCCTGAACC CGGTCGCTCC 
ATGAGCGCCA GCAGTGGCCT ATCTGCAAGG TCATCTGCAC AGAATGGCAG TGCGTTAAAG 
AGAGAATTCT CTGGAGGCAG CTACAGTGCA AAGCGGCAGC CCATGCCCTC CCCATCGGAG 
GGCTCGCTGT CCTCAGGAGG CATGGACCAG GGAAGTGACG CCCCAGCGCG GGACTATGAC 
GGAGAGGACT CTGATTCTCC GCGGCATTCC ACGGCTTCCA ACAGTTCCAA CCTGAGCAGT 
CCTCCCAGCC CGGTGTCGCC CCGGAAGACC AAGAGCCTGT CTCTGGAGAG CACAGACCGC 
GGGAGCTGGG ACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062426.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062488.1 Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X1, mRNA.

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CloneID	OBu18503
Clone ID Related Accession (Same CDS sequence)	XM_006062426.2 , XM_006062426.1
Accession Version	XM_006062426.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGATCCC 
GTTGAGAACA CATATGTACG GAATCCGAAC GTCAAGTTTT ACATCCAGTC AAGGTCCACC 
TCTCCTTCCA CATCTAGTGA AGCTGAGCCA GTTAAGACTG TAGACTCTAC TCCACTTCCA 
GTTCATACAC CAACCTTAAG GAAAAAGGGA TGCCCTGGCT CAACTGGCTT TCCATCGAAG 
CGCAAGATTC ATCAGTTTTT TGTCAAATCT TTTACTACTC CCACCAGGTG TCATCAGTGT 
ACATCTTTGA TGGTGGGTTT GATAAGACAA GGCTGTTCTT GTGAAGTGTG TGGATTTTCA 
TGTCACATAA CTTGTGTAAA CAAAGCTCCA ACTGCTTGTC CAGTTCCTCC TGAACAGACA 
AAAGGTCCTC TGGGTATAGA TCCACAGAAA GGAATAGGAA CAGCATATGA AGGGCATGTC 
AGGATCCCTA AGCCAGCTGG AGTGAAGAAA GGATGGCAAA GAGCACTGGC TGTAGTCTGT 
GACTTCAAGC TCTTTCTGTA CGATATTGCC GAAGGAAAGG CATCTCAGCC CAGTGTTGTA 
GTCAGTCAAG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT CTTGGCTTCT 
GATGTTATTC ATGCAAGTCG AAAAGATATA CCCTGTATAT TTAGAGTCAC AGCTTCCCAG 
CTCTCAGCAT CTAGTAACAA ATGTTCCATC CTGATGCTAG CTGATAGTGA AAATGAGAAG 
AGTAAATGGG TGGGAGTACT GAGTGAATTG CACAAGATTT TGAAGAAAAA CAAATTCAGA 
GACCGCTCAG TCTATGTTCC CAAAGAGGCT TATGACAGCA CTCTGCCCCT CATTAAAACA 
ACCCAGGCAG CTGCAATCAT AGATCATGAA AGAATAGCTT TGGGGAATGA AGAAGGGTTA 
TTTGTTGTGC ATGTCACCAA AGATGAAATT ATTAGAGTTG GTGACAATAA GAAGATTCAT 
CAGATAGAAC TCATTCCAAA TGATCAACTT GTTGCCGTGA TCTCAGGACG AAATCGACAC 
GTCCGACTTT TTCCTATGTC AGCTCTGGAC GGGCGAGAGA CTGATTTTTA TAAGCTGGCA 
GAAACTAAAG GCTGTCAAAC AATAACTTCT GGCAAAGTGC GTCATGGAGC TCTTACATGC 
TTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACGCGT 
CACAGAAAAT TTAAGGAAAT TCAAGTCCCG TACAACGTCC AGTGGATGGC GATCTTCAGT 
GAGCAGCTCT GTGTGGGATT CCAGTCTGGA TTTCTAAGAT ATCCCCTGAA TGGAGAAGGA 
AGTCCATACA GTATGCTCCA TTCAAATGAC AATACACTCT CATTTATTGC ACATCAACCA 
ATGGATGCCA TCTGTGCGGT TGAAATCTCC AGTAAAGAAT ACCTGCTGTG TTTTAACAGC 
ATTGGAATAT ACACTGACTG CCAGGGCCGA AGATCTAGAC AACAGGAACT GATGTGGCCA 
GCGAATCCTT CCTCTTGTTG TTACAATGCA CCGTATCTCT CAGTGTACAG TGAAAACGCA 
GTTGACATCT TTGATGTGAA CTCCATGGAA TGGATTCAGA CTCTCCCACT CAAAAAGGTT 
CGCCCCTTAA ACAGTGAAGG ATCATTAAAT CTTTTAGGCT TGGAGACGAT TAGATTGATA 
TATTTCAAAA ATAAAATGGC AGAAGGGGAT GAACTGGTAG TTCCTGAAAC ATCGGATAAT 
AGCCGGAAGC AAATGGTTAG AAATATTAAC AATAAGAGGC GTTACTCTTT CAGAGTTCCG 
GAAGAGGAAA GAATGCAACA GAGGAGGGAG ATGTTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACATA TGGGTCCAGG AGATGGAATA 
CAGATCCTAA AAGACCTGCC CATGCCGGGT TTCCCTTATC CATCCCCTCA TCATCACTCC 
GGTCTGATCT CCTCTCCGAT CAACTTTGAG CACATCTATC ACATGACTGT TAATTCTGCT 
GAAAAATTTC TCTCTCCTGA TTCCATAAAC CCTGAATATT CCCCGTCTCT GCGCTCTGTC 
CCTGGTACAC CAAGCTTTAT GACTCTGAGG AACCCTCGGC CTCAGGAAAG TCGGACAGCA 
TTCAGTGGCT CGGTCAGTAT TCCGTCTATC ACCAAATCCC GCCCTGAACC CGGTCGCTCC 
ATGAGCGCCA GCAGTGGCCT ATCTGCAAGG TCATCTGCAC AGAATGGCAG TGCGTTAAAG 
AGAGAATTCT CTGGAGGCAG CTACAGTGCA AAGCGGCAGC CCATGCCCTC CCCATCGGAG 
GGCTCGCTGT CCTCAGGAGG CATGGACCAG GGAAGTGACG CCCCAGCGCG GGACTATGAC 
GGAGAGGACT CTGATTCTCC GCGGCATTCC ACGGCTTCCA ACAGTTCCAA CCTGAGCAGT 
CCTCCCAGCC CGGTGTCGCC CCGGAAGACC AAGAGCCTGT CTCTGGAGAG CACAGACCGC 
GGGAGCTGGG ACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062488.1
CDS	1075..6612
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062426.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062426.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGATCCC 
GTTGAGAACA CATATGTACG GAATCCGAAC GTCAAGTTTT ACATCCAGTC AAGGTCCACC 
TCTCCTTCCA CATCTAGTGA AGCTGAGCCA GTTAAGACTG TAGACTCTAC TCCACTTCCA 
GTTCATACAC CAACCTTAAG GAAAAAGGGA TGCCCTGGCT CAACTGGCTT TCCATCGAAG 
CGCAAGATTC ATCAGTTTTT TGTCAAATCT TTTACTACTC CCACCAGGTG TCATCAGTGT 
ACATCTTTGA TGGTGGGTTT GATAAGACAA GGCTGTTCTT GTGAAGTGTG TGGATTTTCA 
TGTCACATAA CTTGTGTAAA CAAAGCTCCA ACTGCTTGTC CAGTTCCTCC TGAACAGACA 
AAAGGTCCTC TGGGTATAGA TCCACAGAAA GGAATAGGAA CAGCATATGA AGGGCATGTC 
AGGATCCCTA AGCCAGCTGG AGTGAAGAAA GGATGGCAAA GAGCACTGGC TGTAGTCTGT 
GACTTCAAGC TCTTTCTGTA CGATATTGCC GAAGGAAAGG CATCTCAGCC CAGTGTTGTA 
GTCAGTCAAG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT CTTGGCTTCT 
GATGTTATTC ATGCAAGTCG AAAAGATATA CCCTGTATAT TTAGAGTCAC AGCTTCCCAG 
CTCTCAGCAT CTAGTAACAA ATGTTCCATC CTGATGCTAG CTGATAGTGA AAATGAGAAG 
AGTAAATGGG TGGGAGTACT GAGTGAATTG CACAAGATTT TGAAGAAAAA CAAATTCAGA 
GACCGCTCAG TCTATGTTCC CAAAGAGGCT TATGACAGCA CTCTGCCCCT CATTAAAACA 
ACCCAGGCAG CTGCAATCAT AGATCATGAA AGAATAGCTT TGGGGAATGA AGAAGGGTTA 
TTTGTTGTGC ATGTCACCAA AGATGAAATT ATTAGAGTTG GTGACAATAA GAAGATTCAT 
CAGATAGAAC TCATTCCAAA TGATCAACTT GTTGCCGTGA TCTCAGGACG AAATCGACAC 
GTCCGACTTT TTCCTATGTC AGCTCTGGAC GGGCGAGAGA CTGATTTTTA TAAGCTGGCA 
GAAACTAAAG GCTGTCAAAC AATAACTTCT GGCAAAGTGC GTCATGGAGC TCTTACATGC 
TTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACGCGT 
CACAGAAAAT TTAAGGAAAT TCAAGTCCCG TACAACGTCC AGTGGATGGC GATCTTCAGT 
GAGCAGCTCT GTGTGGGATT CCAGTCTGGA TTTCTAAGAT ATCCCCTGAA TGGAGAAGGA 
AGTCCATACA GTATGCTCCA TTCAAATGAC AATACACTCT CATTTATTGC ACATCAACCA 
ATGGATGCCA TCTGTGCGGT TGAAATCTCC AGTAAAGAAT ACCTGCTGTG TTTTAACAGC 
ATTGGAATAT ACACTGACTG CCAGGGCCGA AGATCTAGAC AACAGGAACT GATGTGGCCA 
GCGAATCCTT CCTCTTGTTG TTACAATGCA CCGTATCTCT CAGTGTACAG TGAAAACGCA 
GTTGACATCT TTGATGTGAA CTCCATGGAA TGGATTCAGA CTCTCCCACT CAAAAAGGTT 
CGCCCCTTAA ACAGTGAAGG ATCATTAAAT CTTTTAGGCT TGGAGACGAT TAGATTGATA 
TATTTCAAAA ATAAAATGGC AGAAGGGGAT GAACTGGTAG TTCCTGAAAC ATCGGATAAT 
AGCCGGAAGC AAATGGTTAG AAATATTAAC AATAAGAGGC GTTACTCTTT CAGAGTTCCG 
GAAGAGGAAA GAATGCAACA GAGGAGGGAG ATGTTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACATA TGGGTCCAGG AGATGGAATA 
CAGATCCTAA AAGACCTGCC CATGCCGGGT TTCCCTTATC CATCCCCTCA TCATCACTCC 
GGTCTGATCT CCTCTCCGAT CAACTTTGAG CACATCTATC ACATGACTGT TAATTCTGCT 
GAAAAATTTC TCTCTCCTGA TTCCATAAAC CCTGAATATT CCCCGTCTCT GCGCTCTGTC 
CCTGGTACAC CAAGCTTTAT GACTCTGAGG AACCCTCGGC CTCAGGAAAG TCGGACAGCA 
TTCAGTGGCT CGGTCAGTAT TCCGTCTATC ACCAAATCCC GCCCTGAACC CGGTCGCTCC 
ATGAGCGCCA GCAGTGGCCT ATCTGCAAGG TCATCTGCAC AGAATGGCAG TGCGTTAAAG 
AGAGAATTCT CTGGAGGCAG CTACAGTGCA AAGCGGCAGC CCATGCCCTC CCCATCGGAG 
GGCTCGCTGT CCTCAGGAGG CATGGACCAG GGAAGTGACG CCCCAGCGCG GGACTATGAC 
GGAGAGGACT CTGATTCTCC GCGGCATTCC ACGGCTTCCA ACAGTTCCAA CCTGAGCAGT 
CCTCCCAGCC CGGTGTCGCC CCGGAAGACC AAGAGCCTGT CTCTGGAGAG CACAGACCGC 
GGGAGCTGGG ACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062427.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062489.1 Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X2, mRNA.

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CloneID	OBu18504
Clone ID Related Accession (Same CDS sequence)	XM_006062427.2 , XM_006062427.1
Accession Version	XM_006062427.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006062427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGATC CCGTTGAGAA CACATATGTA CGGAATCCGA ACGTCAAGTT TTACATCCAG 
TCAAGGTCCA CCTCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCT 
ACTCCACTTC CAGTTCATAC ACCAACCTTA AGGAAAAAGG GATGCCCTGG CTCAACTGGC 
TTTCCATCGA AGCGCAAGAT TCATCAGTTT TTTGTCAAAT CTTTTACTAC TCCCACCAGG 
TGTCATCAGT GTACATCTTT GATGGTGGGT TTGATAAGAC AAGGCTGTTC TTGTGAAGTG 
TGTGGATTTT CATGTCACAT AACTTGTGTA AACAAAGCTC CAACTGCTTG TCCAGTTCCT 
CCTGAACAGA CAAAAGGTCC TCTGGGTATA GATCCACAGA AAGGAATAGG AACAGCATAT 
GAAGGGCATG TCAGGATCCC TAAGCCAGCT GGAGTGAAGA AAGGATGGCA AAGAGCACTG 
GCTGTAGTCT GTGACTTCAA GCTCTTTCTG TACGATATTG CCGAAGGAAA GGCATCTCAG 
CCCAGTGTTG TAGTCAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA 
GTCTTGGCTT CTGATGTTAT TCATGCAAGT CGAAAAGATA TACCCTGTAT ATTTAGAGTC 
ACAGCTTCCC AGCTCTCAGC ATCTAGTAAC AAATGTTCCA TCCTGATGCT AGCTGATAGT 
GAAAATGAGA AGAGTAAATG GGTGGGAGTA CTGAGTGAAT TGCACAAGAT TTTGAAGAAA 
AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTGCCC 
CTCATTAAAA CAACCCAGGC AGCTGCAATC ATAGATCATG AAAGAATAGC TTTGGGGAAT 
GAAGAAGGGT TATTTGTTGT GCATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT 
AAGAAGATTC ATCAGATAGA ACTCATTCCA AATGATCAAC TTGTTGCCGT GATCTCAGGA 
CGAAATCGAC ACGTCCGACT TTTTCCTATG TCAGCTCTGG ACGGGCGAGA GACTGATTTT 
TATAAGCTGG CAGAAACTAA AGGCTGTCAA ACAATAACTT CTGGCAAAGT GCGTCATGGA 
GCTCTTACAT GCTTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG 
AGCAAGACGC GTCACAGAAA ATTTAAGGAA ATTCAAGTCC CGTACAACGT CCAGTGGATG 
GCGATCTTCA GTGAGCAGCT CTGTGTGGGA TTCCAGTCTG GATTTCTAAG ATATCCCCTG 
AATGGAGAAG GAAGTCCATA CAGTATGCTC CATTCAAATG ACAATACACT CTCATTTATT 
GCACATCAAC CAATGGATGC CATCTGTGCG GTTGAAATCT CCAGTAAAGA ATACCTGCTG 
TGTTTTAACA GCATTGGAAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA 
CTGATGTGGC CAGCGAATCC TTCCTCTTGT TGTTACAATG CACCGTATCT CTCAGTGTAC 
AGTGAAAACG CAGTTGACAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTCCCA 
CTCAAAAAGG TTCGCCCCTT AAACAGTGAA GGATCATTAA ATCTTTTAGG CTTGGAGACG 
ATTAGATTGA TATATTTCAA AAATAAAATG GCAGAAGGGG ATGAACTGGT AGTTCCTGAA 
ACATCGGATA ATAGCCGGAA GCAAATGGTT AGAAATATTA ACAATAAGAG GCGTTACTCT 
TTCAGAGTTC CGGAAGAGGA AAGAATGCAA CAGAGGAGGG AGATGTTACG AGATCCAGAA 
ATGAGAAATA AATTAATTTC TAACCCAACT AATTTTAACC ACATAGCACA TATGGGTCCA 
GGAGATGGAA TACAGATCCT AAAAGACCTG CCCATGCCGG GTTTCCCTTA TCCATCCCCT 
CATCATCACT CCGGTCTGAT CTCCTCTCCG ATCAACTTTG AGCACATCTA TCACATGACT 
GTTAATTCTG CTGAAAAATT TCTCTCTCCT GATTCCATAA ACCCTGAATA TTCCCCGTCT 
CTGCGCTCTG TCCCTGGTAC ACCAAGCTTT ATGACTCTGA GGAACCCTCG GCCTCAGGAA 
AGTCGGACAG CATTCAGTGG CTCGGTCAGT ATTCCGTCTA TCACCAAATC CCGCCCTGAA 
CCCGGTCGCT CCATGAGCGC CAGCAGTGGC CTATCTGCAA GGTCATCTGC ACAGAATGGC 
AGTGCGTTAA AGAGAGAATT CTCTGGAGGC AGCTACAGTG CAAAGCGGCA GCCCATGCCC 
TCCCCATCGG AGGGCTCGCT GTCCTCAGGA GGCATGGACC AGGGAAGTGA CGCCCCAGCG 
CGGGACTATG ACGGAGAGGA CTCTGATTCT CCGCGGCATT CCACGGCTTC CAACAGTTCC 
AACCTGAGCA GTCCTCCCAG CCCGGTGTCG CCCCGGAAGA CCAAGAGCCT GTCTCTGGAG 
AGCACAGACC GCGGGAGCTG GGACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062489.1
CDS	766..6255
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062427.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062427.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGATC CCGTTGAGAA CACATATGTA CGGAATCCGA ACGTCAAGTT TTACATCCAG 
TCAAGGTCCA CCTCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCT 
ACTCCACTTC CAGTTCATAC ACCAACCTTA AGGAAAAAGG GATGCCCTGG CTCAACTGGC 
TTTCCATCGA AGCGCAAGAT TCATCAGTTT TTTGTCAAAT CTTTTACTAC TCCCACCAGG 
TGTCATCAGT GTACATCTTT GATGGTGGGT TTGATAAGAC AAGGCTGTTC TTGTGAAGTG 
TGTGGATTTT CATGTCACAT AACTTGTGTA AACAAAGCTC CAACTGCTTG TCCAGTTCCT 
CCTGAACAGA CAAAAGGTCC TCTGGGTATA GATCCACAGA AAGGAATAGG AACAGCATAT 
GAAGGGCATG TCAGGATCCC TAAGCCAGCT GGAGTGAAGA AAGGATGGCA AAGAGCACTG 
GCTGTAGTCT GTGACTTCAA GCTCTTTCTG TACGATATTG CCGAAGGAAA GGCATCTCAG 
CCCAGTGTTG TAGTCAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA 
GTCTTGGCTT CTGATGTTAT TCATGCAAGT CGAAAAGATA TACCCTGTAT ATTTAGAGTC 
ACAGCTTCCC AGCTCTCAGC ATCTAGTAAC AAATGTTCCA TCCTGATGCT AGCTGATAGT 
GAAAATGAGA AGAGTAAATG GGTGGGAGTA CTGAGTGAAT TGCACAAGAT TTTGAAGAAA 
AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTGCCC 
CTCATTAAAA CAACCCAGGC AGCTGCAATC ATAGATCATG AAAGAATAGC TTTGGGGAAT 
GAAGAAGGGT TATTTGTTGT GCATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT 
AAGAAGATTC ATCAGATAGA ACTCATTCCA AATGATCAAC TTGTTGCCGT GATCTCAGGA 
CGAAATCGAC ACGTCCGACT TTTTCCTATG TCAGCTCTGG ACGGGCGAGA GACTGATTTT 
TATAAGCTGG CAGAAACTAA AGGCTGTCAA ACAATAACTT CTGGCAAAGT GCGTCATGGA 
GCTCTTACAT GCTTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG 
AGCAAGACGC GTCACAGAAA ATTTAAGGAA ATTCAAGTCC CGTACAACGT CCAGTGGATG 
GCGATCTTCA GTGAGCAGCT CTGTGTGGGA TTCCAGTCTG GATTTCTAAG ATATCCCCTG 
AATGGAGAAG GAAGTCCATA CAGTATGCTC CATTCAAATG ACAATACACT CTCATTTATT 
GCACATCAAC CAATGGATGC CATCTGTGCG GTTGAAATCT CCAGTAAAGA ATACCTGCTG 
TGTTTTAACA GCATTGGAAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA 
CTGATGTGGC CAGCGAATCC TTCCTCTTGT TGTTACAATG CACCGTATCT CTCAGTGTAC 
AGTGAAAACG CAGTTGACAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTCCCA 
CTCAAAAAGG TTCGCCCCTT AAACAGTGAA GGATCATTAA ATCTTTTAGG CTTGGAGACG 
ATTAGATTGA TATATTTCAA AAATAAAATG GCAGAAGGGG ATGAACTGGT AGTTCCTGAA 
ACATCGGATA ATAGCCGGAA GCAAATGGTT AGAAATATTA ACAATAAGAG GCGTTACTCT 
TTCAGAGTTC CGGAAGAGGA AAGAATGCAA CAGAGGAGGG AGATGTTACG AGATCCAGAA 
ATGAGAAATA AATTAATTTC TAACCCAACT AATTTTAACC ACATAGCACA TATGGGTCCA 
GGAGATGGAA TACAGATCCT AAAAGACCTG CCCATGCCGG GTTTCCCTTA TCCATCCCCT 
CATCATCACT CCGGTCTGAT CTCCTCTCCG ATCAACTTTG AGCACATCTA TCACATGACT 
GTTAATTCTG CTGAAAAATT TCTCTCTCCT GATTCCATAA ACCCTGAATA TTCCCCGTCT 
CTGCGCTCTG TCCCTGGTAC ACCAAGCTTT ATGACTCTGA GGAACCCTCG GCCTCAGGAA 
AGTCGGACAG CATTCAGTGG CTCGGTCAGT ATTCCGTCTA TCACCAAATC CCGCCCTGAA 
CCCGGTCGCT CCATGAGCGC CAGCAGTGGC CTATCTGCAA GGTCATCTGC ACAGAATGGC 
AGTGCGTTAA AGAGAGAATT CTCTGGAGGC AGCTACAGTG CAAAGCGGCA GCCCATGCCC 
TCCCCATCGG AGGGCTCGCT GTCCTCAGGA GGCATGGACC AGGGAAGTGA CGCCCCAGCG 
CGGGACTATG ACGGAGAGGA CTCTGATTCT CCGCGGCATT CCACGGCTTC CAACAGTTCC 
AACCTGAGCA GTCCTCCCAG CCCGGTGTCG CCCCGGAAGA CCAAGAGCCT GTCTCTGGAG 
AGCACAGACC GCGGGAGCTG GGACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062427.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062489.1 Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X2, mRNA.

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CloneID	OBu18504
Clone ID Related Accession (Same CDS sequence)	XM_006062427.2 , XM_006062427.1
Accession Version	XM_006062427.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGATC CCGTTGAGAA CACATATGTA CGGAATCCGA ACGTCAAGTT TTACATCCAG 
TCAAGGTCCA CCTCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCT 
ACTCCACTTC CAGTTCATAC ACCAACCTTA AGGAAAAAGG GATGCCCTGG CTCAACTGGC 
TTTCCATCGA AGCGCAAGAT TCATCAGTTT TTTGTCAAAT CTTTTACTAC TCCCACCAGG 
TGTCATCAGT GTACATCTTT GATGGTGGGT TTGATAAGAC AAGGCTGTTC TTGTGAAGTG 
TGTGGATTTT CATGTCACAT AACTTGTGTA AACAAAGCTC CAACTGCTTG TCCAGTTCCT 
CCTGAACAGA CAAAAGGTCC TCTGGGTATA GATCCACAGA AAGGAATAGG AACAGCATAT 
GAAGGGCATG TCAGGATCCC TAAGCCAGCT GGAGTGAAGA AAGGATGGCA AAGAGCACTG 
GCTGTAGTCT GTGACTTCAA GCTCTTTCTG TACGATATTG CCGAAGGAAA GGCATCTCAG 
CCCAGTGTTG TAGTCAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA 
GTCTTGGCTT CTGATGTTAT TCATGCAAGT CGAAAAGATA TACCCTGTAT ATTTAGAGTC 
ACAGCTTCCC AGCTCTCAGC ATCTAGTAAC AAATGTTCCA TCCTGATGCT AGCTGATAGT 
GAAAATGAGA AGAGTAAATG GGTGGGAGTA CTGAGTGAAT TGCACAAGAT TTTGAAGAAA 
AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTGCCC 
CTCATTAAAA CAACCCAGGC AGCTGCAATC ATAGATCATG AAAGAATAGC TTTGGGGAAT 
GAAGAAGGGT TATTTGTTGT GCATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT 
AAGAAGATTC ATCAGATAGA ACTCATTCCA AATGATCAAC TTGTTGCCGT GATCTCAGGA 
CGAAATCGAC ACGTCCGACT TTTTCCTATG TCAGCTCTGG ACGGGCGAGA GACTGATTTT 
TATAAGCTGG CAGAAACTAA AGGCTGTCAA ACAATAACTT CTGGCAAAGT GCGTCATGGA 
GCTCTTACAT GCTTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG 
AGCAAGACGC GTCACAGAAA ATTTAAGGAA ATTCAAGTCC CGTACAACGT CCAGTGGATG 
GCGATCTTCA GTGAGCAGCT CTGTGTGGGA TTCCAGTCTG GATTTCTAAG ATATCCCCTG 
AATGGAGAAG GAAGTCCATA CAGTATGCTC CATTCAAATG ACAATACACT CTCATTTATT 
GCACATCAAC CAATGGATGC CATCTGTGCG GTTGAAATCT CCAGTAAAGA ATACCTGCTG 
TGTTTTAACA GCATTGGAAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA 
CTGATGTGGC CAGCGAATCC TTCCTCTTGT TGTTACAATG CACCGTATCT CTCAGTGTAC 
AGTGAAAACG CAGTTGACAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTCCCA 
CTCAAAAAGG TTCGCCCCTT AAACAGTGAA GGATCATTAA ATCTTTTAGG CTTGGAGACG 
ATTAGATTGA TATATTTCAA AAATAAAATG GCAGAAGGGG ATGAACTGGT AGTTCCTGAA 
ACATCGGATA ATAGCCGGAA GCAAATGGTT AGAAATATTA ACAATAAGAG GCGTTACTCT 
TTCAGAGTTC CGGAAGAGGA AAGAATGCAA CAGAGGAGGG AGATGTTACG AGATCCAGAA 
ATGAGAAATA AATTAATTTC TAACCCAACT AATTTTAACC ACATAGCACA TATGGGTCCA 
GGAGATGGAA TACAGATCCT AAAAGACCTG CCCATGCCGG GTTTCCCTTA TCCATCCCCT 
CATCATCACT CCGGTCTGAT CTCCTCTCCG ATCAACTTTG AGCACATCTA TCACATGACT 
GTTAATTCTG CTGAAAAATT TCTCTCTCCT GATTCCATAA ACCCTGAATA TTCCCCGTCT 
CTGCGCTCTG TCCCTGGTAC ACCAAGCTTT ATGACTCTGA GGAACCCTCG GCCTCAGGAA 
AGTCGGACAG CATTCAGTGG CTCGGTCAGT ATTCCGTCTA TCACCAAATC CCGCCCTGAA 
CCCGGTCGCT CCATGAGCGC CAGCAGTGGC CTATCTGCAA GGTCATCTGC ACAGAATGGC 
AGTGCGTTAA AGAGAGAATT CTCTGGAGGC AGCTACAGTG CAAAGCGGCA GCCCATGCCC 
TCCCCATCGG AGGGCTCGCT GTCCTCAGGA GGCATGGACC AGGGAAGTGA CGCCCCAGCG 
CGGGACTATG ACGGAGAGGA CTCTGATTCT CCGCGGCATT CCACGGCTTC CAACAGTTCC 
AACCTGAGCA GTCCTCCCAG CCCGGTGTCG CCCCGGAAGA CCAAGAGCCT GTCTCTGGAG 
AGCACAGACC GCGGGAGCTG GGACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062489.1
CDS	1075..6564
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062427.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062427.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGATC CCGTTGAGAA CACATATGTA CGGAATCCGA ACGTCAAGTT TTACATCCAG 
TCAAGGTCCA CCTCTCCTTC CACATCTAGT GAAGCTGAGC CAGTTAAGAC TGTAGACTCT 
ACTCCACTTC CAGTTCATAC ACCAACCTTA AGGAAAAAGG GATGCCCTGG CTCAACTGGC 
TTTCCATCGA AGCGCAAGAT TCATCAGTTT TTTGTCAAAT CTTTTACTAC TCCCACCAGG 
TGTCATCAGT GTACATCTTT GATGGTGGGT TTGATAAGAC AAGGCTGTTC TTGTGAAGTG 
TGTGGATTTT CATGTCACAT AACTTGTGTA AACAAAGCTC CAACTGCTTG TCCAGTTCCT 
CCTGAACAGA CAAAAGGTCC TCTGGGTATA GATCCACAGA AAGGAATAGG AACAGCATAT 
GAAGGGCATG TCAGGATCCC TAAGCCAGCT GGAGTGAAGA AAGGATGGCA AAGAGCACTG 
GCTGTAGTCT GTGACTTCAA GCTCTTTCTG TACGATATTG CCGAAGGAAA GGCATCTCAG 
CCCAGTGTTG TAGTCAGTCA AGTGATTGAC ATGAGGGATG AAGAATTTTC TGTGAGTTCA 
GTCTTGGCTT CTGATGTTAT TCATGCAAGT CGAAAAGATA TACCCTGTAT ATTTAGAGTC 
ACAGCTTCCC AGCTCTCAGC ATCTAGTAAC AAATGTTCCA TCCTGATGCT AGCTGATAGT 
GAAAATGAGA AGAGTAAATG GGTGGGAGTA CTGAGTGAAT TGCACAAGAT TTTGAAGAAA 
AACAAATTCA GAGACCGCTC AGTCTATGTT CCCAAAGAGG CTTATGACAG CACTCTGCCC 
CTCATTAAAA CAACCCAGGC AGCTGCAATC ATAGATCATG AAAGAATAGC TTTGGGGAAT 
GAAGAAGGGT TATTTGTTGT GCATGTCACC AAAGATGAAA TTATTAGAGT TGGTGACAAT 
AAGAAGATTC ATCAGATAGA ACTCATTCCA AATGATCAAC TTGTTGCCGT GATCTCAGGA 
CGAAATCGAC ACGTCCGACT TTTTCCTATG TCAGCTCTGG ACGGGCGAGA GACTGATTTT 
TATAAGCTGG CAGAAACTAA AGGCTGTCAA ACAATAACTT CTGGCAAAGT GCGTCATGGA 
GCTCTTACAT GCTTGTGTGT GGCTATGAAA AGGCAGGTCC TCTGTTATGA ACTATTTCAG 
AGCAAGACGC GTCACAGAAA ATTTAAGGAA ATTCAAGTCC CGTACAACGT CCAGTGGATG 
GCGATCTTCA GTGAGCAGCT CTGTGTGGGA TTCCAGTCTG GATTTCTAAG ATATCCCCTG 
AATGGAGAAG GAAGTCCATA CAGTATGCTC CATTCAAATG ACAATACACT CTCATTTATT 
GCACATCAAC CAATGGATGC CATCTGTGCG GTTGAAATCT CCAGTAAAGA ATACCTGCTG 
TGTTTTAACA GCATTGGAAT ATACACTGAC TGCCAGGGCC GAAGATCTAG ACAACAGGAA 
CTGATGTGGC CAGCGAATCC TTCCTCTTGT TGTTACAATG CACCGTATCT CTCAGTGTAC 
AGTGAAAACG CAGTTGACAT CTTTGATGTG AACTCCATGG AATGGATTCA GACTCTCCCA 
CTCAAAAAGG TTCGCCCCTT AAACAGTGAA GGATCATTAA ATCTTTTAGG CTTGGAGACG 
ATTAGATTGA TATATTTCAA AAATAAAATG GCAGAAGGGG ATGAACTGGT AGTTCCTGAA 
ACATCGGATA ATAGCCGGAA GCAAATGGTT AGAAATATTA ACAATAAGAG GCGTTACTCT 
TTCAGAGTTC CGGAAGAGGA AAGAATGCAA CAGAGGAGGG AGATGTTACG AGATCCAGAA 
ATGAGAAATA AATTAATTTC TAACCCAACT AATTTTAACC ACATAGCACA TATGGGTCCA 
GGAGATGGAA TACAGATCCT AAAAGACCTG CCCATGCCGG GTTTCCCTTA TCCATCCCCT 
CATCATCACT CCGGTCTGAT CTCCTCTCCG ATCAACTTTG AGCACATCTA TCACATGACT 
GTTAATTCTG CTGAAAAATT TCTCTCTCCT GATTCCATAA ACCCTGAATA TTCCCCGTCT 
CTGCGCTCTG TCCCTGGTAC ACCAAGCTTT ATGACTCTGA GGAACCCTCG GCCTCAGGAA 
AGTCGGACAG CATTCAGTGG CTCGGTCAGT ATTCCGTCTA TCACCAAATC CCGCCCTGAA 
CCCGGTCGCT CCATGAGCGC CAGCAGTGGC CTATCTGCAA GGTCATCTGC ACAGAATGGC 
AGTGCGTTAA AGAGAGAATT CTCTGGAGGC AGCTACAGTG CAAAGCGGCA GCCCATGCCC 
TCCCCATCGG AGGGCTCGCT GTCCTCAGGA GGCATGGACC AGGGAAGTGA CGCCCCAGCG 
CGGGACTATG ACGGAGAGGA CTCTGATTCT CCGCGGCATT CCACGGCTTC CAACAGTTCC 
AACCTGAGCA GTCCTCCCAG CCCGGTGTCG CCCCGGAAGA CCAAGAGCCT GTCTCTGGAG 
AGCACAGACC GCGGGAGCTG GGACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062428.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062490.1 Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X3, mRNA.

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CloneID	OBu18505
Clone ID Related Accession (Same CDS sequence)	XM_006062428.1 , XM_006062428.2
Accession Version	XM_006062428.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGAT CCCGTTGAGA ACACATATGT ACGGAATCCG 
AACGTCAAGT TTTACATCCA GTCAAGGTCC ACCTCTCCTT CCACATCTAG TGAAGCTGAG 
CCAGTTAAGA CTGTAGACTC TACTCCACTT CCAGTTCATA CACCAACCTT AAGGAAAAAG 
GGATGCCCTG GCTCAACTGG CTTTCCATCG AAGCGCAAGA TTCATCAGTT TTTTGTCAAA 
TCTTTTACTA CTCCCACCAG GTGTCATCAG TGTACATCTT TGATGGTGGG TTTGATAAGA 
CAAGGCTGTT CTTGTGAAGT GTGTGGATTT TCATGTCACA TAACTTGTGT AAACAAAGCT 
CCAACTGCTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CTCTGGGTAT AGATCCACAG 
AAAGGAATAG GAACAGCATA TGAAGGGCAT GTCAGGATCC CTAAGCCAGC TGGAGTGAAG 
AAAGGATGGC AAAGAGCACT GGCTGTAGTC TGTGACTTCA AGCTCTTTCT GTACGATATT 
GCCGAAGGAA AGGCATCTCA GCCCAGTGTT GTAGTCAGTC AAGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TTCATGCAAG TCGAAAAGAT 
ATACCCTGTA TATTTAGAGT CACAGCTTCC CAGCTCTCAG CATCTAGTAA CAAATGTTCC 
ATCCTGATGC TAGCTGATAG TGAAAATGAG AAGAGTAAAT GGGTGGGAGT ACTGAGTGAA 
TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG 
GCTTATGACA GCACTCTGCC CCTCATTAAA ACAACCCAGG CAGCTGCAAT CATAGATCAT 
GAAAGAATAG CTTTGGGGAA TGAAGAAGGG TTATTTGTTG TGCATGTCAC CAAAGATGAA 
ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATAG AACTCATTCC AAATGATCAA 
CTTGTTGCCG TGATCTCAGG ACGAAATCGA CACGTCCGAC TTTTTCCTAT GTCAGCTCTG 
GACGGGCGAG AGACTGATTT TTATAAGCTG GCAGAAACTA AAGGCTGTCA AACAATAACT 
TCTGGCAAAG TGCGTCATGG AGCTCTTACA TGCTTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACG CGTCACAGAA AATTTAAGGA AATTCAAGTC 
CCGTACAACG TCCAGTGGAT GGCGATCTTC AGTGAGCAGC TCTGTGTGGG ATTCCAGTCT 
GGATTTCTAA GATATCCCCT GAATGGAGAA GGAAGTCCAT ACAGTATGCT CCATTCAAAT 
GACAATACAC TCTCATTTAT TGCACATCAA CCAATGGATG CCATCTGTGC GGTTGAAATC 
TCCAGTAAAG AATACCTGCT GTGTTTTAAC AGCATTGGAA TATACACTGA CTGCCAGGGC 
CGAAGATCTA GACAACAGGA ACTGATGTGG CCAGCGAATC CTTCCTCTTG TTGTTACAAT 
GCACCGTATC TCTCAGTGTA CAGTGAAAAC GCAGTTGACA TCTTTGATGT GAACTCCATG 
GAATGGATTC AGACTCTCCC ACTCAAAAAG GTTCGCCCCT TAAACAGTGA AGGATCATTA 
AATCTTTTAG GCTTGGAGAC GATTAGATTG ATATATTTCA AAAATAAAAT GGCAGAAGGG 
GATGAACTGG TAGTTCCTGA AACATCGGAT AATAGCCGGA AGCAAATGGT TAGAAATATT 
AACAATAAGA GGCGTTACTC TTTCAGAGTT CCGGAAGAGG AAAGAATGCA ACAGAGGAGG 
GAGATGTTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAACCCAAC TAATTTTAAC 
CACATAGCAC ATATGGGTCC AGGAGATGGA ATACAGATCC TAAAAGACCT GCCCATGCCG 
GGTTTCCCTT ATCCATCCCC TCATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT 
GAGCACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA 
AACCCTGAAT ATTCCCCGTC TCTGCGCTCT GTCCCTGGTA CACCAAGCTT TATGACTCTG 
AGGAACCCTC GGCCTCAGGA AAGTCGGACA GCATTCAGTG GCTCGGTCAG TATTCCGTCT 
ATCACCAAAT CCCGCCCTGA ACCCGGTCGC TCCATGAGCG CCAGCAGTGG CCTATCTGCA 
AGGTCATCTG CACAGAATGG CAGTGCGTTA AAGAGAGAAT TCTCTGGAGG CAGCTACAGT 
GCAAAGCGGC AGCCCATGCC CTCCCCATCG GAGGGCTCGC TGTCCTCAGG AGGCATGGAC 
CAGGGAAGTG ACGCCCCAGC GCGGGACTAT GACGGAGAGG ACTCTGATTC TCCGCGGCAT 
TCCACGGCTT CCAACAGTTC CAACCTGAGC AGTCCTCCCA GCCCGGTGTC GCCCCGGAAG 
ACCAAGAGCC TGTCTCTGGA GAGCACAGAC CGCGGGAGCT GGGACCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062490.1
CDS	291..5741
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062428.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062428.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGAT CCCGTTGAGA ACACATATGT ACGGAATCCG 
AACGTCAAGT TTTACATCCA GTCAAGGTCC ACCTCTCCTT CCACATCTAG TGAAGCTGAG 
CCAGTTAAGA CTGTAGACTC TACTCCACTT CCAGTTCATA CACCAACCTT AAGGAAAAAG 
GGATGCCCTG GCTCAACTGG CTTTCCATCG AAGCGCAAGA TTCATCAGTT TTTTGTCAAA 
TCTTTTACTA CTCCCACCAG GTGTCATCAG TGTACATCTT TGATGGTGGG TTTGATAAGA 
CAAGGCTGTT CTTGTGAAGT GTGTGGATTT TCATGTCACA TAACTTGTGT AAACAAAGCT 
CCAACTGCTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CTCTGGGTAT AGATCCACAG 
AAAGGAATAG GAACAGCATA TGAAGGGCAT GTCAGGATCC CTAAGCCAGC TGGAGTGAAG 
AAAGGATGGC AAAGAGCACT GGCTGTAGTC TGTGACTTCA AGCTCTTTCT GTACGATATT 
GCCGAAGGAA AGGCATCTCA GCCCAGTGTT GTAGTCAGTC AAGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TTCATGCAAG TCGAAAAGAT 
ATACCCTGTA TATTTAGAGT CACAGCTTCC CAGCTCTCAG CATCTAGTAA CAAATGTTCC 
ATCCTGATGC TAGCTGATAG TGAAAATGAG AAGAGTAAAT GGGTGGGAGT ACTGAGTGAA 
TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG 
GCTTATGACA GCACTCTGCC CCTCATTAAA ACAACCCAGG CAGCTGCAAT CATAGATCAT 
GAAAGAATAG CTTTGGGGAA TGAAGAAGGG TTATTTGTTG TGCATGTCAC CAAAGATGAA 
ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATAG AACTCATTCC AAATGATCAA 
CTTGTTGCCG TGATCTCAGG ACGAAATCGA CACGTCCGAC TTTTTCCTAT GTCAGCTCTG 
GACGGGCGAG AGACTGATTT TTATAAGCTG GCAGAAACTA AAGGCTGTCA AACAATAACT 
TCTGGCAAAG TGCGTCATGG AGCTCTTACA TGCTTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACG CGTCACAGAA AATTTAAGGA AATTCAAGTC 
CCGTACAACG TCCAGTGGAT GGCGATCTTC AGTGAGCAGC TCTGTGTGGG ATTCCAGTCT 
GGATTTCTAA GATATCCCCT GAATGGAGAA GGAAGTCCAT ACAGTATGCT CCATTCAAAT 
GACAATACAC TCTCATTTAT TGCACATCAA CCAATGGATG CCATCTGTGC GGTTGAAATC 
TCCAGTAAAG AATACCTGCT GTGTTTTAAC AGCATTGGAA TATACACTGA CTGCCAGGGC 
CGAAGATCTA GACAACAGGA ACTGATGTGG CCAGCGAATC CTTCCTCTTG TTGTTACAAT 
GCACCGTATC TCTCAGTGTA CAGTGAAAAC GCAGTTGACA TCTTTGATGT GAACTCCATG 
GAATGGATTC AGACTCTCCC ACTCAAAAAG GTTCGCCCCT TAAACAGTGA AGGATCATTA 
AATCTTTTAG GCTTGGAGAC GATTAGATTG ATATATTTCA AAAATAAAAT GGCAGAAGGG 
GATGAACTGG TAGTTCCTGA AACATCGGAT AATAGCCGGA AGCAAATGGT TAGAAATATT 
AACAATAAGA GGCGTTACTC TTTCAGAGTT CCGGAAGAGG AAAGAATGCA ACAGAGGAGG 
GAGATGTTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAACCCAAC TAATTTTAAC 
CACATAGCAC ATATGGGTCC AGGAGATGGA ATACAGATCC TAAAAGACCT GCCCATGCCG 
GGTTTCCCTT ATCCATCCCC TCATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT 
GAGCACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA 
AACCCTGAAT ATTCCCCGTC TCTGCGCTCT GTCCCTGGTA CACCAAGCTT TATGACTCTG 
AGGAACCCTC GGCCTCAGGA AAGTCGGACA GCATTCAGTG GCTCGGTCAG TATTCCGTCT 
ATCACCAAAT CCCGCCCTGA ACCCGGTCGC TCCATGAGCG CCAGCAGTGG CCTATCTGCA 
AGGTCATCTG CACAGAATGG CAGTGCGTTA AAGAGAGAAT TCTCTGGAGG CAGCTACAGT 
GCAAAGCGGC AGCCCATGCC CTCCCCATCG GAGGGCTCGC TGTCCTCAGG AGGCATGGAC 
CAGGGAAGTG ACGCCCCAGC GCGGGACTAT GACGGAGAGG ACTCTGATTC TCCGCGGCAT 
TCCACGGCTT CCAACAGTTC CAACCTGAGC AGTCCTCCCA GCCCGGTGTC GCCCCGGAAG 
ACCAAGAGCC TGTCTCTGGA GAGCACAGAC CGCGGGAGCT GGGACCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062428.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062490.1 Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X3, mRNA.

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CloneID	OBu18505
Clone ID Related Accession (Same CDS sequence)	XM_006062428.1 , XM_006062428.2
Accession Version	XM_006062428.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006062428.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGAT CCCGTTGAGA ACACATATGT ACGGAATCCG 
AACGTCAAGT TTTACATCCA GTCAAGGTCC ACCTCTCCTT CCACATCTAG TGAAGCTGAG 
CCAGTTAAGA CTGTAGACTC TACTCCACTT CCAGTTCATA CACCAACCTT AAGGAAAAAG 
GGATGCCCTG GCTCAACTGG CTTTCCATCG AAGCGCAAGA TTCATCAGTT TTTTGTCAAA 
TCTTTTACTA CTCCCACCAG GTGTCATCAG TGTACATCTT TGATGGTGGG TTTGATAAGA 
CAAGGCTGTT CTTGTGAAGT GTGTGGATTT TCATGTCACA TAACTTGTGT AAACAAAGCT 
CCAACTGCTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CTCTGGGTAT AGATCCACAG 
AAAGGAATAG GAACAGCATA TGAAGGGCAT GTCAGGATCC CTAAGCCAGC TGGAGTGAAG 
AAAGGATGGC AAAGAGCACT GGCTGTAGTC TGTGACTTCA AGCTCTTTCT GTACGATATT 
GCCGAAGGAA AGGCATCTCA GCCCAGTGTT GTAGTCAGTC AAGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TTCATGCAAG TCGAAAAGAT 
ATACCCTGTA TATTTAGAGT CACAGCTTCC CAGCTCTCAG CATCTAGTAA CAAATGTTCC 
ATCCTGATGC TAGCTGATAG TGAAAATGAG AAGAGTAAAT GGGTGGGAGT ACTGAGTGAA 
TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG 
GCTTATGACA GCACTCTGCC CCTCATTAAA ACAACCCAGG CAGCTGCAAT CATAGATCAT 
GAAAGAATAG CTTTGGGGAA TGAAGAAGGG TTATTTGTTG TGCATGTCAC CAAAGATGAA 
ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATAG AACTCATTCC AAATGATCAA 
CTTGTTGCCG TGATCTCAGG ACGAAATCGA CACGTCCGAC TTTTTCCTAT GTCAGCTCTG 
GACGGGCGAG AGACTGATTT TTATAAGCTG GCAGAAACTA AAGGCTGTCA AACAATAACT 
TCTGGCAAAG TGCGTCATGG AGCTCTTACA TGCTTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACG CGTCACAGAA AATTTAAGGA AATTCAAGTC 
CCGTACAACG TCCAGTGGAT GGCGATCTTC AGTGAGCAGC TCTGTGTGGG ATTCCAGTCT 
GGATTTCTAA GATATCCCCT GAATGGAGAA GGAAGTCCAT ACAGTATGCT CCATTCAAAT 
GACAATACAC TCTCATTTAT TGCACATCAA CCAATGGATG CCATCTGTGC GGTTGAAATC 
TCCAGTAAAG AATACCTGCT GTGTTTTAAC AGCATTGGAA TATACACTGA CTGCCAGGGC 
CGAAGATCTA GACAACAGGA ACTGATGTGG CCAGCGAATC CTTCCTCTTG TTGTTACAAT 
GCACCGTATC TCTCAGTGTA CAGTGAAAAC GCAGTTGACA TCTTTGATGT GAACTCCATG 
GAATGGATTC AGACTCTCCC ACTCAAAAAG GTTCGCCCCT TAAACAGTGA AGGATCATTA 
AATCTTTTAG GCTTGGAGAC GATTAGATTG ATATATTTCA AAAATAAAAT GGCAGAAGGG 
GATGAACTGG TAGTTCCTGA AACATCGGAT AATAGCCGGA AGCAAATGGT TAGAAATATT 
AACAATAAGA GGCGTTACTC TTTCAGAGTT CCGGAAGAGG AAAGAATGCA ACAGAGGAGG 
GAGATGTTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAACCCAAC TAATTTTAAC 
CACATAGCAC ATATGGGTCC AGGAGATGGA ATACAGATCC TAAAAGACCT GCCCATGCCG 
GGTTTCCCTT ATCCATCCCC TCATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT 
GAGCACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA 
AACCCTGAAT ATTCCCCGTC TCTGCGCTCT GTCCCTGGTA CACCAAGCTT TATGACTCTG 
AGGAACCCTC GGCCTCAGGA AAGTCGGACA GCATTCAGTG GCTCGGTCAG TATTCCGTCT 
ATCACCAAAT CCCGCCCTGA ACCCGGTCGC TCCATGAGCG CCAGCAGTGG CCTATCTGCA 
AGGTCATCTG CACAGAATGG CAGTGCGTTA AAGAGAGAAT TCTCTGGAGG CAGCTACAGT 
GCAAAGCGGC AGCCCATGCC CTCCCCATCG GAGGGCTCGC TGTCCTCAGG AGGCATGGAC 
CAGGGAAGTG ACGCCCCAGC GCGGGACTAT GACGGAGAGG ACTCTGATTC TCCGCGGCAT 
TCCACGGCTT CCAACAGTTC CAACCTGAGC AGTCCTCCCA GCCCGGTGTC GCCCCGGAAG 
ACCAAGAGCC TGTCTCTGGA GAGCACAGAC CGCGGGAGCT GGGACCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062490.1
CDS	766..6216
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062428.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062428.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGAT CCCGTTGAGA ACACATATGT ACGGAATCCG 
AACGTCAAGT TTTACATCCA GTCAAGGTCC ACCTCTCCTT CCACATCTAG TGAAGCTGAG 
CCAGTTAAGA CTGTAGACTC TACTCCACTT CCAGTTCATA CACCAACCTT AAGGAAAAAG 
GGATGCCCTG GCTCAACTGG CTTTCCATCG AAGCGCAAGA TTCATCAGTT TTTTGTCAAA 
TCTTTTACTA CTCCCACCAG GTGTCATCAG TGTACATCTT TGATGGTGGG TTTGATAAGA 
CAAGGCTGTT CTTGTGAAGT GTGTGGATTT TCATGTCACA TAACTTGTGT AAACAAAGCT 
CCAACTGCTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CTCTGGGTAT AGATCCACAG 
AAAGGAATAG GAACAGCATA TGAAGGGCAT GTCAGGATCC CTAAGCCAGC TGGAGTGAAG 
AAAGGATGGC AAAGAGCACT GGCTGTAGTC TGTGACTTCA AGCTCTTTCT GTACGATATT 
GCCGAAGGAA AGGCATCTCA GCCCAGTGTT GTAGTCAGTC AAGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TTCATGCAAG TCGAAAAGAT 
ATACCCTGTA TATTTAGAGT CACAGCTTCC CAGCTCTCAG CATCTAGTAA CAAATGTTCC 
ATCCTGATGC TAGCTGATAG TGAAAATGAG AAGAGTAAAT GGGTGGGAGT ACTGAGTGAA 
TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG 
GCTTATGACA GCACTCTGCC CCTCATTAAA ACAACCCAGG CAGCTGCAAT CATAGATCAT 
GAAAGAATAG CTTTGGGGAA TGAAGAAGGG TTATTTGTTG TGCATGTCAC CAAAGATGAA 
ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATAG AACTCATTCC AAATGATCAA 
CTTGTTGCCG TGATCTCAGG ACGAAATCGA CACGTCCGAC TTTTTCCTAT GTCAGCTCTG 
GACGGGCGAG AGACTGATTT TTATAAGCTG GCAGAAACTA AAGGCTGTCA AACAATAACT 
TCTGGCAAAG TGCGTCATGG AGCTCTTACA TGCTTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACG CGTCACAGAA AATTTAAGGA AATTCAAGTC 
CCGTACAACG TCCAGTGGAT GGCGATCTTC AGTGAGCAGC TCTGTGTGGG ATTCCAGTCT 
GGATTTCTAA GATATCCCCT GAATGGAGAA GGAAGTCCAT ACAGTATGCT CCATTCAAAT 
GACAATACAC TCTCATTTAT TGCACATCAA CCAATGGATG CCATCTGTGC GGTTGAAATC 
TCCAGTAAAG AATACCTGCT GTGTTTTAAC AGCATTGGAA TATACACTGA CTGCCAGGGC 
CGAAGATCTA GACAACAGGA ACTGATGTGG CCAGCGAATC CTTCCTCTTG TTGTTACAAT 
GCACCGTATC TCTCAGTGTA CAGTGAAAAC GCAGTTGACA TCTTTGATGT GAACTCCATG 
GAATGGATTC AGACTCTCCC ACTCAAAAAG GTTCGCCCCT TAAACAGTGA AGGATCATTA 
AATCTTTTAG GCTTGGAGAC GATTAGATTG ATATATTTCA AAAATAAAAT GGCAGAAGGG 
GATGAACTGG TAGTTCCTGA AACATCGGAT AATAGCCGGA AGCAAATGGT TAGAAATATT 
AACAATAAGA GGCGTTACTC TTTCAGAGTT CCGGAAGAGG AAAGAATGCA ACAGAGGAGG 
GAGATGTTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAACCCAAC TAATTTTAAC 
CACATAGCAC ATATGGGTCC AGGAGATGGA ATACAGATCC TAAAAGACCT GCCCATGCCG 
GGTTTCCCTT ATCCATCCCC TCATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT 
GAGCACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA 
AACCCTGAAT ATTCCCCGTC TCTGCGCTCT GTCCCTGGTA CACCAAGCTT TATGACTCTG 
AGGAACCCTC GGCCTCAGGA AAGTCGGACA GCATTCAGTG GCTCGGTCAG TATTCCGTCT 
ATCACCAAAT CCCGCCCTGA ACCCGGTCGC TCCATGAGCG CCAGCAGTGG CCTATCTGCA 
AGGTCATCTG CACAGAATGG CAGTGCGTTA AAGAGAGAAT TCTCTGGAGG CAGCTACAGT 
GCAAAGCGGC AGCCCATGCC CTCCCCATCG GAGGGCTCGC TGTCCTCAGG AGGCATGGAC 
CAGGGAAGTG ACGCCCCAGC GCGGGACTAT GACGGAGAGG ACTCTGATTC TCCGCGGCAT 
TCCACGGCTT CCAACAGTTC CAACCTGAGC AGTCCTCCCA GCCCGGTGTC GCCCCGGAAG 
ACCAAGAGCC TGTCTCTGGA GAGCACAGAC CGCGGGAGCT GGGACCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062429.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062491.1 Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X4, mRNA.

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CloneID	OBu18506
Clone ID Related Accession (Same CDS sequence)	XM_006062429.2 , XM_006062429.1
Accession Version	XM_006062429.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006062429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGTAGAC 
TCTACTCCAC TTCCAGTTCA TACACCAACC TTAAGGAAAA AGGGATGCCC TGGCTCAACT 
GGCTTTCCAT CGAAGCGCAA GATTCATCAG TTTTTTGTCA AATCTTTTAC TACTCCCACC 
AGGTGTCATC AGTGTACATC TTTGATGGTG GGTTTGATAA GACAAGGCTG TTCTTGTGAA 
GTGTGTGGAT TTTCATGTCA CATAACTTGT GTAAACAAAG CTCCAACTGC TTGTCCAGTT 
CCTCCTGAAC AGACAAAAGG TCCTCTGGGT ATAGATCCAC AGAAAGGAAT AGGAACAGCA 
TATGAAGGGC ATGTCAGGAT CCCTAAGCCA GCTGGAGTGA AGAAAGGATG GCAAAGAGCA 
CTGGCTGTAG TCTGTGACTT CAAGCTCTTT CTGTACGATA TTGCCGAAGG AAAGGCATCT 
CAGCCCAGTG TTGTAGTCAG TCAAGTGATT GACATGAGGG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTGG CTTCTGATGT TATTCATGCA AGTCGAAAAG ATATACCCTG TATATTTAGA 
GTCACAGCTT CCCAGCTCTC AGCATCTAGT AACAAATGTT CCATCCTGAT GCTAGCTGAT 
AGTGAAAATG AGAAGAGTAA ATGGGTGGGA GTACTGAGTG AATTGCACAA GATTTTGAAG 
AAAAACAAAT TCAGAGACCG CTCAGTCTAT GTTCCCAAAG AGGCTTATGA CAGCACTCTG 
CCCCTCATTA AAACAACCCA GGCAGCTGCA ATCATAGATC ATGAAAGAAT AGCTTTGGGG 
AATGAAGAAG GGTTATTTGT TGTGCATGTC ACCAAAGATG AAATTATTAG AGTTGGTGAC 
AATAAGAAGA TTCATCAGAT AGAACTCATT CCAAATGATC AACTTGTTGC CGTGATCTCA 
GGACGAAATC GACACGTCCG ACTTTTTCCT ATGTCAGCTC TGGACGGGCG AGAGACTGAT 
TTTTATAAGC TGGCAGAAAC TAAAGGCTGT CAAACAATAA CTTCTGGCAA AGTGCGTCAT 
GGAGCTCTTA CATGCTTGTG TGTGGCTATG AAAAGGCAGG TCCTCTGTTA TGAACTATTT 
CAGAGCAAGA CGCGTCACAG AAAATTTAAG GAAATTCAAG TCCCGTACAA CGTCCAGTGG 
ATGGCGATCT TCAGTGAGCA GCTCTGTGTG GGATTCCAGT CTGGATTTCT AAGATATCCC 
CTGAATGGAG AAGGAAGTCC ATACAGTATG CTCCATTCAA ATGACAATAC ACTCTCATTT 
ATTGCACATC AACCAATGGA TGCCATCTGT GCGGTTGAAA TCTCCAGTAA AGAATACCTG 
CTGTGTTTTA ACAGCATTGG AATATACACT GACTGCCAGG GCCGAAGATC TAGACAACAG 
GAACTGATGT GGCCAGCGAA TCCTTCCTCT TGTTGTTACA ATGCACCGTA TCTCTCAGTG 
TACAGTGAAA ACGCAGTTGA CATCTTTGAT GTGAACTCCA TGGAATGGAT TCAGACTCTC 
CCACTCAAAA AGGTTCGCCC CTTAAACAGT GAAGGATCAT TAAATCTTTT AGGCTTGGAG 
ACGATTAGAT TGATATATTT CAAAAATAAA ATGGCAGAAG GGGATGAACT GGTAGTTCCT 
GAAACATCGG ATAATAGCCG GAAGCAAATG GTTAGAAATA TTAACAATAA GAGGCGTTAC 
TCTTTCAGAG TTCCGGAAGA GGAAAGAATG CAACAGAGGA GGGAGATGTT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAACCCA ACTAATTTTA ACCACATAGC ACATATGGGT 
CCAGGAGATG GAATACAGAT CCTAAAAGAC CTGCCCATGC CGGGTTTCCC TTATCCATCC 
CCTCATCATC ACTCCGGTCT GATCTCCTCT CCGATCAACT TTGAGCACAT CTATCACATG 
ACTGTTAATT CTGCTGAAAA ATTTCTCTCT CCTGATTCCA TAAACCCTGA ATATTCCCCG 
TCTCTGCGCT CTGTCCCTGG TACACCAAGC TTTATGACTC TGAGGAACCC TCGGCCTCAG 
GAAAGTCGGA CAGCATTCAG TGGCTCGGTC AGTATTCCGT CTATCACCAA ATCCCGCCCT 
GAACCCGGTC GCTCCATGAG CGCCAGCAGT GGCCTATCTG CAAGGTCATC TGCACAGAAT 
GGCAGTGCGT TAAAGAGAGA ATTCTCTGGA GGCAGCTACA GTGCAAAGCG GCAGCCCATG 
CCCTCCCCAT CGGAGGGCTC GCTGTCCTCA GGAGGCATGG ACCAGGGAAG TGACGCCCCA 
GCGCGGGACT ATGACGGAGA GGACTCTGAT TCTCCGCGGC ATTCCACGGC TTCCAACAGT 
TCCAACCTGA GCAGTCCTCC CAGCCCGGTG TCGCCCCGGA AGACCAAGAG CCTGTCTCTG 
GAGAGCACAG ACCGCGGGAG CTGGGACCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062491.1
CDS	766..6198
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062429.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062429.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGTAGAC 
TCTACTCCAC TTCCAGTTCA TACACCAACC TTAAGGAAAA AGGGATGCCC TGGCTCAACT 
GGCTTTCCAT CGAAGCGCAA GATTCATCAG TTTTTTGTCA AATCTTTTAC TACTCCCACC 
AGGTGTCATC AGTGTACATC TTTGATGGTG GGTTTGATAA GACAAGGCTG TTCTTGTGAA 
GTGTGTGGAT TTTCATGTCA CATAACTTGT GTAAACAAAG CTCCAACTGC TTGTCCAGTT 
CCTCCTGAAC AGACAAAAGG TCCTCTGGGT ATAGATCCAC AGAAAGGAAT AGGAACAGCA 
TATGAAGGGC ATGTCAGGAT CCCTAAGCCA GCTGGAGTGA AGAAAGGATG GCAAAGAGCA 
CTGGCTGTAG TCTGTGACTT CAAGCTCTTT CTGTACGATA TTGCCGAAGG AAAGGCATCT 
CAGCCCAGTG TTGTAGTCAG TCAAGTGATT GACATGAGGG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTGG CTTCTGATGT TATTCATGCA AGTCGAAAAG ATATACCCTG TATATTTAGA 
GTCACAGCTT CCCAGCTCTC AGCATCTAGT AACAAATGTT CCATCCTGAT GCTAGCTGAT 
AGTGAAAATG AGAAGAGTAA ATGGGTGGGA GTACTGAGTG AATTGCACAA GATTTTGAAG 
AAAAACAAAT TCAGAGACCG CTCAGTCTAT GTTCCCAAAG AGGCTTATGA CAGCACTCTG 
CCCCTCATTA AAACAACCCA GGCAGCTGCA ATCATAGATC ATGAAAGAAT AGCTTTGGGG 
AATGAAGAAG GGTTATTTGT TGTGCATGTC ACCAAAGATG AAATTATTAG AGTTGGTGAC 
AATAAGAAGA TTCATCAGAT AGAACTCATT CCAAATGATC AACTTGTTGC CGTGATCTCA 
GGACGAAATC GACACGTCCG ACTTTTTCCT ATGTCAGCTC TGGACGGGCG AGAGACTGAT 
TTTTATAAGC TGGCAGAAAC TAAAGGCTGT CAAACAATAA CTTCTGGCAA AGTGCGTCAT 
GGAGCTCTTA CATGCTTGTG TGTGGCTATG AAAAGGCAGG TCCTCTGTTA TGAACTATTT 
CAGAGCAAGA CGCGTCACAG AAAATTTAAG GAAATTCAAG TCCCGTACAA CGTCCAGTGG 
ATGGCGATCT TCAGTGAGCA GCTCTGTGTG GGATTCCAGT CTGGATTTCT AAGATATCCC 
CTGAATGGAG AAGGAAGTCC ATACAGTATG CTCCATTCAA ATGACAATAC ACTCTCATTT 
ATTGCACATC AACCAATGGA TGCCATCTGT GCGGTTGAAA TCTCCAGTAA AGAATACCTG 
CTGTGTTTTA ACAGCATTGG AATATACACT GACTGCCAGG GCCGAAGATC TAGACAACAG 
GAACTGATGT GGCCAGCGAA TCCTTCCTCT TGTTGTTACA ATGCACCGTA TCTCTCAGTG 
TACAGTGAAA ACGCAGTTGA CATCTTTGAT GTGAACTCCA TGGAATGGAT TCAGACTCTC 
CCACTCAAAA AGGTTCGCCC CTTAAACAGT GAAGGATCAT TAAATCTTTT AGGCTTGGAG 
ACGATTAGAT TGATATATTT CAAAAATAAA ATGGCAGAAG GGGATGAACT GGTAGTTCCT 
GAAACATCGG ATAATAGCCG GAAGCAAATG GTTAGAAATA TTAACAATAA GAGGCGTTAC 
TCTTTCAGAG TTCCGGAAGA GGAAAGAATG CAACAGAGGA GGGAGATGTT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAACCCA ACTAATTTTA ACCACATAGC ACATATGGGT 
CCAGGAGATG GAATACAGAT CCTAAAAGAC CTGCCCATGC CGGGTTTCCC TTATCCATCC 
CCTCATCATC ACTCCGGTCT GATCTCCTCT CCGATCAACT TTGAGCACAT CTATCACATG 
ACTGTTAATT CTGCTGAAAA ATTTCTCTCT CCTGATTCCA TAAACCCTGA ATATTCCCCG 
TCTCTGCGCT CTGTCCCTGG TACACCAAGC TTTATGACTC TGAGGAACCC TCGGCCTCAG 
GAAAGTCGGA CAGCATTCAG TGGCTCGGTC AGTATTCCGT CTATCACCAA ATCCCGCCCT 
GAACCCGGTC GCTCCATGAG CGCCAGCAGT GGCCTATCTG CAAGGTCATC TGCACAGAAT 
GGCAGTGCGT TAAAGAGAGA ATTCTCTGGA GGCAGCTACA GTGCAAAGCG GCAGCCCATG 
CCCTCCCCAT CGGAGGGCTC GCTGTCCTCA GGAGGCATGG ACCAGGGAAG TGACGCCCCA 
GCGCGGGACT ATGACGGAGA GGACTCTGAT TCTCCGCGGC ATTCCACGGC TTCCAACAGT 
TCCAACCTGA GCAGTCCTCC CAGCCCGGTG TCGCCCCGGA AGACCAAGAG CCTGTCTCTG 
GAGAGCACAG ACCGCGGGAG CTGGGACCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062429.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062491.1 Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X4, mRNA.

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CloneID	OBu18506
Clone ID Related Accession (Same CDS sequence)	XM_006062429.2 , XM_006062429.1
Accession Version	XM_006062429.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGTAGAC 
TCTACTCCAC TTCCAGTTCA TACACCAACC TTAAGGAAAA AGGGATGCCC TGGCTCAACT 
GGCTTTCCAT CGAAGCGCAA GATTCATCAG TTTTTTGTCA AATCTTTTAC TACTCCCACC 
AGGTGTCATC AGTGTACATC TTTGATGGTG GGTTTGATAA GACAAGGCTG TTCTTGTGAA 
GTGTGTGGAT TTTCATGTCA CATAACTTGT GTAAACAAAG CTCCAACTGC TTGTCCAGTT 
CCTCCTGAAC AGACAAAAGG TCCTCTGGGT ATAGATCCAC AGAAAGGAAT AGGAACAGCA 
TATGAAGGGC ATGTCAGGAT CCCTAAGCCA GCTGGAGTGA AGAAAGGATG GCAAAGAGCA 
CTGGCTGTAG TCTGTGACTT CAAGCTCTTT CTGTACGATA TTGCCGAAGG AAAGGCATCT 
CAGCCCAGTG TTGTAGTCAG TCAAGTGATT GACATGAGGG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTGG CTTCTGATGT TATTCATGCA AGTCGAAAAG ATATACCCTG TATATTTAGA 
GTCACAGCTT CCCAGCTCTC AGCATCTAGT AACAAATGTT CCATCCTGAT GCTAGCTGAT 
AGTGAAAATG AGAAGAGTAA ATGGGTGGGA GTACTGAGTG AATTGCACAA GATTTTGAAG 
AAAAACAAAT TCAGAGACCG CTCAGTCTAT GTTCCCAAAG AGGCTTATGA CAGCACTCTG 
CCCCTCATTA AAACAACCCA GGCAGCTGCA ATCATAGATC ATGAAAGAAT AGCTTTGGGG 
AATGAAGAAG GGTTATTTGT TGTGCATGTC ACCAAAGATG AAATTATTAG AGTTGGTGAC 
AATAAGAAGA TTCATCAGAT AGAACTCATT CCAAATGATC AACTTGTTGC CGTGATCTCA 
GGACGAAATC GACACGTCCG ACTTTTTCCT ATGTCAGCTC TGGACGGGCG AGAGACTGAT 
TTTTATAAGC TGGCAGAAAC TAAAGGCTGT CAAACAATAA CTTCTGGCAA AGTGCGTCAT 
GGAGCTCTTA CATGCTTGTG TGTGGCTATG AAAAGGCAGG TCCTCTGTTA TGAACTATTT 
CAGAGCAAGA CGCGTCACAG AAAATTTAAG GAAATTCAAG TCCCGTACAA CGTCCAGTGG 
ATGGCGATCT TCAGTGAGCA GCTCTGTGTG GGATTCCAGT CTGGATTTCT AAGATATCCC 
CTGAATGGAG AAGGAAGTCC ATACAGTATG CTCCATTCAA ATGACAATAC ACTCTCATTT 
ATTGCACATC AACCAATGGA TGCCATCTGT GCGGTTGAAA TCTCCAGTAA AGAATACCTG 
CTGTGTTTTA ACAGCATTGG AATATACACT GACTGCCAGG GCCGAAGATC TAGACAACAG 
GAACTGATGT GGCCAGCGAA TCCTTCCTCT TGTTGTTACA ATGCACCGTA TCTCTCAGTG 
TACAGTGAAA ACGCAGTTGA CATCTTTGAT GTGAACTCCA TGGAATGGAT TCAGACTCTC 
CCACTCAAAA AGGTTCGCCC CTTAAACAGT GAAGGATCAT TAAATCTTTT AGGCTTGGAG 
ACGATTAGAT TGATATATTT CAAAAATAAA ATGGCAGAAG GGGATGAACT GGTAGTTCCT 
GAAACATCGG ATAATAGCCG GAAGCAAATG GTTAGAAATA TTAACAATAA GAGGCGTTAC 
TCTTTCAGAG TTCCGGAAGA GGAAAGAATG CAACAGAGGA GGGAGATGTT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAACCCA ACTAATTTTA ACCACATAGC ACATATGGGT 
CCAGGAGATG GAATACAGAT CCTAAAAGAC CTGCCCATGC CGGGTTTCCC TTATCCATCC 
CCTCATCATC ACTCCGGTCT GATCTCCTCT CCGATCAACT TTGAGCACAT CTATCACATG 
ACTGTTAATT CTGCTGAAAA ATTTCTCTCT CCTGATTCCA TAAACCCTGA ATATTCCCCG 
TCTCTGCGCT CTGTCCCTGG TACACCAAGC TTTATGACTC TGAGGAACCC TCGGCCTCAG 
GAAAGTCGGA CAGCATTCAG TGGCTCGGTC AGTATTCCGT CTATCACCAA ATCCCGCCCT 
GAACCCGGTC GCTCCATGAG CGCCAGCAGT GGCCTATCTG CAAGGTCATC TGCACAGAAT 
GGCAGTGCGT TAAAGAGAGA ATTCTCTGGA GGCAGCTACA GTGCAAAGCG GCAGCCCATG 
CCCTCCCCAT CGGAGGGCTC GCTGTCCTCA GGAGGCATGG ACCAGGGAAG TGACGCCCCA 
GCGCGGGACT ATGACGGAGA GGACTCTGAT TCTCCGCGGC ATTCCACGGC TTCCAACAGT 
TCCAACCTGA GCAGTCCTCC CAGCCCGGTG TCGCCCCGGA AGACCAAGAG CCTGTCTCTG 
GAGAGCACAG ACCGCGGGAG CTGGGACCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062491.1
CDS	291..5723
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062429.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062429.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGTAGAC 
TCTACTCCAC TTCCAGTTCA TACACCAACC TTAAGGAAAA AGGGATGCCC TGGCTCAACT 
GGCTTTCCAT CGAAGCGCAA GATTCATCAG TTTTTTGTCA AATCTTTTAC TACTCCCACC 
AGGTGTCATC AGTGTACATC TTTGATGGTG GGTTTGATAA GACAAGGCTG TTCTTGTGAA 
GTGTGTGGAT TTTCATGTCA CATAACTTGT GTAAACAAAG CTCCAACTGC TTGTCCAGTT 
CCTCCTGAAC AGACAAAAGG TCCTCTGGGT ATAGATCCAC AGAAAGGAAT AGGAACAGCA 
TATGAAGGGC ATGTCAGGAT CCCTAAGCCA GCTGGAGTGA AGAAAGGATG GCAAAGAGCA 
CTGGCTGTAG TCTGTGACTT CAAGCTCTTT CTGTACGATA TTGCCGAAGG AAAGGCATCT 
CAGCCCAGTG TTGTAGTCAG TCAAGTGATT GACATGAGGG ATGAAGAATT TTCTGTGAGT 
TCAGTCTTGG CTTCTGATGT TATTCATGCA AGTCGAAAAG ATATACCCTG TATATTTAGA 
GTCACAGCTT CCCAGCTCTC AGCATCTAGT AACAAATGTT CCATCCTGAT GCTAGCTGAT 
AGTGAAAATG AGAAGAGTAA ATGGGTGGGA GTACTGAGTG AATTGCACAA GATTTTGAAG 
AAAAACAAAT TCAGAGACCG CTCAGTCTAT GTTCCCAAAG AGGCTTATGA CAGCACTCTG 
CCCCTCATTA AAACAACCCA GGCAGCTGCA ATCATAGATC ATGAAAGAAT AGCTTTGGGG 
AATGAAGAAG GGTTATTTGT TGTGCATGTC ACCAAAGATG AAATTATTAG AGTTGGTGAC 
AATAAGAAGA TTCATCAGAT AGAACTCATT CCAAATGATC AACTTGTTGC CGTGATCTCA 
GGACGAAATC GACACGTCCG ACTTTTTCCT ATGTCAGCTC TGGACGGGCG AGAGACTGAT 
TTTTATAAGC TGGCAGAAAC TAAAGGCTGT CAAACAATAA CTTCTGGCAA AGTGCGTCAT 
GGAGCTCTTA CATGCTTGTG TGTGGCTATG AAAAGGCAGG TCCTCTGTTA TGAACTATTT 
CAGAGCAAGA CGCGTCACAG AAAATTTAAG GAAATTCAAG TCCCGTACAA CGTCCAGTGG 
ATGGCGATCT TCAGTGAGCA GCTCTGTGTG GGATTCCAGT CTGGATTTCT AAGATATCCC 
CTGAATGGAG AAGGAAGTCC ATACAGTATG CTCCATTCAA ATGACAATAC ACTCTCATTT 
ATTGCACATC AACCAATGGA TGCCATCTGT GCGGTTGAAA TCTCCAGTAA AGAATACCTG 
CTGTGTTTTA ACAGCATTGG AATATACACT GACTGCCAGG GCCGAAGATC TAGACAACAG 
GAACTGATGT GGCCAGCGAA TCCTTCCTCT TGTTGTTACA ATGCACCGTA TCTCTCAGTG 
TACAGTGAAA ACGCAGTTGA CATCTTTGAT GTGAACTCCA TGGAATGGAT TCAGACTCTC 
CCACTCAAAA AGGTTCGCCC CTTAAACAGT GAAGGATCAT TAAATCTTTT AGGCTTGGAG 
ACGATTAGAT TGATATATTT CAAAAATAAA ATGGCAGAAG GGGATGAACT GGTAGTTCCT 
GAAACATCGG ATAATAGCCG GAAGCAAATG GTTAGAAATA TTAACAATAA GAGGCGTTAC 
TCTTTCAGAG TTCCGGAAGA GGAAAGAATG CAACAGAGGA GGGAGATGTT ACGAGATCCA 
GAAATGAGAA ATAAATTAAT TTCTAACCCA ACTAATTTTA ACCACATAGC ACATATGGGT 
CCAGGAGATG GAATACAGAT CCTAAAAGAC CTGCCCATGC CGGGTTTCCC TTATCCATCC 
CCTCATCATC ACTCCGGTCT GATCTCCTCT CCGATCAACT TTGAGCACAT CTATCACATG 
ACTGTTAATT CTGCTGAAAA ATTTCTCTCT CCTGATTCCA TAAACCCTGA ATATTCCCCG 
TCTCTGCGCT CTGTCCCTGG TACACCAAGC TTTATGACTC TGAGGAACCC TCGGCCTCAG 
GAAAGTCGGA CAGCATTCAG TGGCTCGGTC AGTATTCCGT CTATCACCAA ATCCCGCCCT 
GAACCCGGTC GCTCCATGAG CGCCAGCAGT GGCCTATCTG CAAGGTCATC TGCACAGAAT 
GGCAGTGCGT TAAAGAGAGA ATTCTCTGGA GGCAGCTACA GTGCAAAGCG GCAGCCCATG 
CCCTCCCCAT CGGAGGGCTC GCTGTCCTCA GGAGGCATGG ACCAGGGAAG TGACGCCCCA 
GCGCGGGACT ATGACGGAGA GGACTCTGAT TCTCCGCGGC ATTCCACGGC TTCCAACAGT 
TCCAACCTGA GCAGTCCTCC CAGCCCGGTG TCGCCCCGGA AGACCAAGAG CCTGTCTCTG 
GAGAGCACAG ACCGCGGGAG CTGGGACCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062430.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062492.1 Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X5, mRNA.

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CloneID	OBu18507
Clone ID Related Accession (Same CDS sequence)	XM_006062430.1
Accession Version	XM_006062430.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTGGCCAA GAGACTGATC CCGTTGAGAA CACATATGTA 
CGGAATCCGA ACGTCAAGTT TTACATCCAG TCAAGGTCCA CCTCTCCTTC CACATCTAGT 
GAAGCTGAGC CAGTTAAGAC TGTAGACTCT ACTCCACTTC CAGTTCATAC ACCAACCTTA 
AGGAAAAAGG GATGCCCTGG CTCAACTGGC TTTCCATCGA AGCGCAAGAT TCATCAGTTT 
TTTGTCAAAT CTTTTACTAC TCCCACCAGG TGTCATCAGT GTACATCTTT GATGGTGGGT 
TTGATAAGAC AAGGCTGTTC TTGTGAAGTG TGTGGATTTT CATGTCACAT AACTTGTGTA 
AACAAAGCTC CAACTGCTTG TCCAGTTCCT CCTGAACAGA CAAAAGGTCC TCTGGGTATA 
GATCCACAGA AAGGAATAGG AACAGCATAT GAAGGGCATG TCAGGATCCC TAAGCCAGCT 
GGAGTGAAGA AAGGATGGCA AAGAGCACTG GCTGTAGTCT GTGACTTCAA GCTCTTTCTG 
TACGATATTG CCGAAGGAAA GGCATCTCAG CCCAGTGTTG TAGTCAGTCA AGTGATTGAC 
ATGAGGGATG AAGAATTTTC TGTGAGTTCA GTCTTGGCTT CTGATGTTAT TCATGCAAGT 
CGAAAAGATA TACCCTGTAT ATTTAGAGTC ACAGCTTCCC AGCTCTCAGC ATCTAGTAAC 
AAATGTTCCA TCCTGATGCT AGCTGATAGT GAAAATGAGA AGAGTAAATG GGTGGGAGTA 
CTGAGTGAAT TGCACAAGAT TTTGAAGAAA AACAAATTCA GAGACCGCTC AGTCTATGTT 
CCCAAAGAGG CTTATGACAG CACTCTGCCC CTCATTAAAA CAACCCAGGC AGCTGCAATC 
ATAGATCATG AAAGAATAGC TTTGGGGAAT GAAGAAGGGT TATTTGTTGT GCATGTCACC 
AAAGATGAAA TTATTAGAGT TGGTGACAAT AAGAAGATTC ATCAGATAGA ACTCATTCCA 
AATGATCAAC TTGTTGCCGT GATCTCAGGA CGAAATCGAC ACGTCCGACT TTTTCCTATG 
TCAGCTCTGG ACGGGCGAGA GACTGATTTT TATAAGCTGG CAGAAACTAA AGGCTGTCAA 
ACAATAACTT CTGGCAAAGT GCGTCATGGA GCTCTTACAT GCTTGTGTGT GGCTATGAAA 
AGGCAGGTCC TCTGTTATGA ACTATTTCAG AGCAAGACGC GTCACAGAAA ATTTAAGGAA 
ATTCAAGTCC CGTACAACGT CCAGTGGATG GCGATCTTCA GTGAGCAGCT CTGTGTGGGA 
TTCCAGTCTG GATTTCTAAG ATATCCCCTG AATGGAGAAG GAAGTCCATA CAGTATGCTC 
CATTCAAATG ACAATACACT CTCATTTATT GCACATCAAC CAATGGATGC CATCTGTGCG 
GTTGAAATCT CCAGTAAAGA ATACCTGCTG TGTTTTAACA GCATTGGAAT ATACACTGAC 
TGCCAGGGCC GAAGATCTAG ACAACAGGAA CTGATGTGGC CAGCGAATCC TTCCTCTTGT 
TGTTACAATG CACCGTATCT CTCAGTGTAC AGTGAAAACG CAGTTGACAT CTTTGATGTG 
AACTCCATGG AATGGATTCA GACTCTCCCA CTCAAAAAGG TTCGCCCCTT AAACAGTGAA 
GGATCATTAA ATCTTTTAGG CTTGGAGACG ATTAGATTGA TATATTTCAA AAATAAAATG 
GCAGAAGGGG ATGAACTGGT AGTTCCTGAA ACATCGGATA ATAGCCGGAA GCAAATGGTT 
AGAAATATTA ACAATAAGAG GCGTTACTCT TTCAGAGTTC CGGAAGAGGA AAGAATGCAA 
CAGAGGAGGG AGATGTTACG AGATCCAGAA ATGAGAAATA AATTAATTTC TAACCCAACT 
AATTTTAACC ACATAGCACA TATGGGTCCA GGAGATGGAA TACAGATCCT AAAAGACCTG 
CCCATGCCGG GTTTCCCTTA TCCATCCCCT CATCATCACT CCGGTCTGAT CTCCTCTCCG 
ATCAACTTTG AGCACATCTA TCACATGACT GTTAATTCTG CTGAAAAATT TCTCTCTCCT 
GATTCCATAA ACCCTGAATA TTCCCCGTCT CTGCGCTCTG TCCCTGGTAC ACCAAGCTTT 
ATGACTCTGA GGAACCCTCG GCCTCAGGAA AGTCGGACAG CATTCAGTGG CTCGGTCAGT 
ATTCCGTCTA TCACCAAATC CCGCCCTGAA CCCGGTCGCT CCATGAGCGC CAGCAGTGGC 
CTATCTGCAA GGTCATCTGC ACAGAATGGC AGTGCGTTAA AGAGAGAATT CTCTGGAGGC 
AGCTACAGTG CAAAGCGGCA GCCCATGCCC TCCCCATCGG AGGGCTCGCT GTCCTCAGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCG CGGGACTATG ACGGAGAGGA CTCTGATTCT 
CCGCGGCATT CCACGGCTTC CAACAGTTCC AACCTGAGCA GTCCTCCCAG CCCGGTGTCG 
CCCCGGAAGA CCAAGAGCCT GTCTCTGGAG AGCACAGACC GCGGGAGCTG GGACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062492.1
CDS	1075..6474
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKASKGRRGQETDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062430.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062430.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTGGCCAA GAGACTGATC CCGTTGAGAA CACATATGTA 
CGGAATCCGA ACGTCAAGTT TTACATCCAG TCAAGGTCCA CCTCTCCTTC CACATCTAGT 
GAAGCTGAGC CAGTTAAGAC TGTAGACTCT ACTCCACTTC CAGTTCATAC ACCAACCTTA 
AGGAAAAAGG GATGCCCTGG CTCAACTGGC TTTCCATCGA AGCGCAAGAT TCATCAGTTT 
TTTGTCAAAT CTTTTACTAC TCCCACCAGG TGTCATCAGT GTACATCTTT GATGGTGGGT 
TTGATAAGAC AAGGCTGTTC TTGTGAAGTG TGTGGATTTT CATGTCACAT AACTTGTGTA 
AACAAAGCTC CAACTGCTTG TCCAGTTCCT CCTGAACAGA CAAAAGGTCC TCTGGGTATA 
GATCCACAGA AAGGAATAGG AACAGCATAT GAAGGGCATG TCAGGATCCC TAAGCCAGCT 
GGAGTGAAGA AAGGATGGCA AAGAGCACTG GCTGTAGTCT GTGACTTCAA GCTCTTTCTG 
TACGATATTG CCGAAGGAAA GGCATCTCAG CCCAGTGTTG TAGTCAGTCA AGTGATTGAC 
ATGAGGGATG AAGAATTTTC TGTGAGTTCA GTCTTGGCTT CTGATGTTAT TCATGCAAGT 
CGAAAAGATA TACCCTGTAT ATTTAGAGTC ACAGCTTCCC AGCTCTCAGC ATCTAGTAAC 
AAATGTTCCA TCCTGATGCT AGCTGATAGT GAAAATGAGA AGAGTAAATG GGTGGGAGTA 
CTGAGTGAAT TGCACAAGAT TTTGAAGAAA AACAAATTCA GAGACCGCTC AGTCTATGTT 
CCCAAAGAGG CTTATGACAG CACTCTGCCC CTCATTAAAA CAACCCAGGC AGCTGCAATC 
ATAGATCATG AAAGAATAGC TTTGGGGAAT GAAGAAGGGT TATTTGTTGT GCATGTCACC 
AAAGATGAAA TTATTAGAGT TGGTGACAAT AAGAAGATTC ATCAGATAGA ACTCATTCCA 
AATGATCAAC TTGTTGCCGT GATCTCAGGA CGAAATCGAC ACGTCCGACT TTTTCCTATG 
TCAGCTCTGG ACGGGCGAGA GACTGATTTT TATAAGCTGG CAGAAACTAA AGGCTGTCAA 
ACAATAACTT CTGGCAAAGT GCGTCATGGA GCTCTTACAT GCTTGTGTGT GGCTATGAAA 
AGGCAGGTCC TCTGTTATGA ACTATTTCAG AGCAAGACGC GTCACAGAAA ATTTAAGGAA 
ATTCAAGTCC CGTACAACGT CCAGTGGATG GCGATCTTCA GTGAGCAGCT CTGTGTGGGA 
TTCCAGTCTG GATTTCTAAG ATATCCCCTG AATGGAGAAG GAAGTCCATA CAGTATGCTC 
CATTCAAATG ACAATACACT CTCATTTATT GCACATCAAC CAATGGATGC CATCTGTGCG 
GTTGAAATCT CCAGTAAAGA ATACCTGCTG TGTTTTAACA GCATTGGAAT ATACACTGAC 
TGCCAGGGCC GAAGATCTAG ACAACAGGAA CTGATGTGGC CAGCGAATCC TTCCTCTTGT 
TGTTACAATG CACCGTATCT CTCAGTGTAC AGTGAAAACG CAGTTGACAT CTTTGATGTG 
AACTCCATGG AATGGATTCA GACTCTCCCA CTCAAAAAGG TTCGCCCCTT AAACAGTGAA 
GGATCATTAA ATCTTTTAGG CTTGGAGACG ATTAGATTGA TATATTTCAA AAATAAAATG 
GCAGAAGGGG ATGAACTGGT AGTTCCTGAA ACATCGGATA ATAGCCGGAA GCAAATGGTT 
AGAAATATTA ACAATAAGAG GCGTTACTCT TTCAGAGTTC CGGAAGAGGA AAGAATGCAA 
CAGAGGAGGG AGATGTTACG AGATCCAGAA ATGAGAAATA AATTAATTTC TAACCCAACT 
AATTTTAACC ACATAGCACA TATGGGTCCA GGAGATGGAA TACAGATCCT AAAAGACCTG 
CCCATGCCGG GTTTCCCTTA TCCATCCCCT CATCATCACT CCGGTCTGAT CTCCTCTCCG 
ATCAACTTTG AGCACATCTA TCACATGACT GTTAATTCTG CTGAAAAATT TCTCTCTCCT 
GATTCCATAA ACCCTGAATA TTCCCCGTCT CTGCGCTCTG TCCCTGGTAC ACCAAGCTTT 
ATGACTCTGA GGAACCCTCG GCCTCAGGAA AGTCGGACAG CATTCAGTGG CTCGGTCAGT 
ATTCCGTCTA TCACCAAATC CCGCCCTGAA CCCGGTCGCT CCATGAGCGC CAGCAGTGGC 
CTATCTGCAA GGTCATCTGC ACAGAATGGC AGTGCGTTAA AGAGAGAATT CTCTGGAGGC 
AGCTACAGTG CAAAGCGGCA GCCCATGCCC TCCCCATCGG AGGGCTCGCT GTCCTCAGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCG CGGGACTATG ACGGAGAGGA CTCTGATTCT 
CCGCGGCATT CCACGGCTTC CAACAGTTCC AACCTGAGCA GTCCTCCCAG CCCGGTGTCG 
CCCCGGAAGA CCAAGAGCCT GTCTCTGGAG AGCACAGACC GCGGGAGCTG GGACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062431.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062493.1 Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X6, mRNA.

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CloneID	OBu18508
Clone ID Related Accession (Same CDS sequence)	XM_006062431.1 , XM_006062431.2
Accession Version	XM_006062431.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACACTGTAGA CTCTACTCCA CTTCCAGTTC ATACACCAAC CTTAAGGAAA 
AAGGGATGCC CTGGCTCAAC TGGCTTTCCA TCGAAGCGCA AGATTCATCA GTTTTTTGTC 
AAATCTTTTA CTACTCCCAC CAGGTGTCAT CAGTGTACAT CTTTGATGGT GGGTTTGATA 
AGACAAGGCT GTTCTTGTGA AGTGTGTGGA TTTTCATGTC ACATAACTTG TGTAAACAAA 
GCTCCAACTG CTTGTCCAGT TCCTCCTGAA CAGACAAAAG GTCCTCTGGG TATAGATCCA 
CAGAAAGGAA TAGGAACAGC ATATGAAGGG CATGTCAGGA TCCCTAAGCC AGCTGGAGTG 
AAGAAAGGAT GGCAAAGAGC ACTGGCTGTA GTCTGTGACT TCAAGCTCTT TCTGTACGAT 
ATTGCCGAAG GAAAGGCATC TCAGCCCAGT GTTGTAGTCA GTCAAGTGAT TGACATGAGG 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTG GCTTCTGATG TTATTCATGC AAGTCGAAAA 
GATATACCCT GTATATTTAG AGTCACAGCT TCCCAGCTCT CAGCATCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCTGA TAGTGAAAAT GAGAAGAGTA AATGGGTGGG AGTACTGAGT 
GAATTGCACA AGATTTTGAA GAAAAACAAA TTCAGAGACC GCTCAGTCTA TGTTCCCAAA 
GAGGCTTATG ACAGCACTCT GCCCCTCATT AAAACAACCC AGGCAGCTGC AATCATAGAT 
CATGAAAGAA TAGCTTTGGG GAATGAAGAA GGGTTATTTG TTGTGCATGT CACCAAAGAT 
GAAATTATTA GAGTTGGTGA CAATAAGAAG ATTCATCAGA TAGAACTCAT TCCAAATGAT 
CAACTTGTTG CCGTGATCTC AGGACGAAAT CGACACGTCC GACTTTTTCC TATGTCAGCT 
CTGGACGGGC GAGAGACTGA TTTTTATAAG CTGGCAGAAA CTAAAGGCTG TCAAACAATA 
ACTTCTGGCA AAGTGCGTCA TGGAGCTCTT ACATGCTTGT GTGTGGCTAT GAAAAGGCAG 
GTCCTCTGTT ATGAACTATT TCAGAGCAAG ACGCGTCACA GAAAATTTAA GGAAATTCAA 
GTCCCGTACA ACGTCCAGTG GATGGCGATC TTCAGTGAGC AGCTCTGTGT GGGATTCCAG 
TCTGGATTTC TAAGATATCC CCTGAATGGA GAAGGAAGTC CATACAGTAT GCTCCATTCA 
AATGACAATA CACTCTCATT TATTGCACAT CAACCAATGG ATGCCATCTG TGCGGTTGAA 
ATCTCCAGTA AAGAATACCT GCTGTGTTTT AACAGCATTG GAATATACAC TGACTGCCAG 
GGCCGAAGAT CTAGACAACA GGAACTGATG TGGCCAGCGA ATCCTTCCTC TTGTTGTTAC 
AATGCACCGT ATCTCTCAGT GTACAGTGAA AACGCAGTTG ACATCTTTGA TGTGAACTCC 
ATGGAATGGA TTCAGACTCT CCCACTCAAA AAGGTTCGCC CCTTAAACAG TGAAGGATCA 
TTAAATCTTT TAGGCTTGGA GACGATTAGA TTGATATATT TCAAAAATAA AATGGCAGAA 
GGGGATGAAC TGGTAGTTCC TGAAACATCG GATAATAGCC GGAAGCAAAT GGTTAGAAAT 
ATTAACAATA AGAGGCGTTA CTCTTTCAGA GTTCCGGAAG AGGAAAGAAT GCAACAGAGG 
AGGGAGATGT TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACATATGGG TCCAGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
CCGGGTTTCC CTTATCCATC CCCTCATCAT CACTCCGGTC TGATCTCCTC TCCGATCAAC 
TTTGAGCACA TCTATCACAT GACTGTTAAT TCTGCTGAAA AATTTCTCTC TCCTGATTCC 
ATAAACCCTG AATATTCCCC GTCTCTGCGC TCTGTCCCTG GTACACCAAG CTTTATGACT 
CTGAGGAACC CTCGGCCTCA GGAAAGTCGG ACAGCATTCA GTGGCTCGGT CAGTATTCCG 
TCTATCACCA AATCCCGCCC TGAACCCGGT CGCTCCATGA GCGCCAGCAG TGGCCTATCT 
GCAAGGTCAT CTGCACAGAA TGGCAGTGCG TTAAAGAGAG AATTCTCTGG AGGCAGCTAC 
AGTGCAAAGC GGCAGCCCAT GCCCTCCCCA TCGGAGGGCT CGCTGTCCTC AGGAGGCATG 
GACCAGGGAA GTGACGCCCC AGCGCGGGAC TATGACGGAG AGGACTCTGA TTCTCCGCGG 
CATTCCACGG CTTCCAACAG TTCCAACCTG AGCAGTCCTC CCAGCCCGGT GTCGCCCCGG 
AAGACCAAGA GCCTGTCTCT GGAGAGCACA GACCGCGGGA GCTGGGACCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062493.1
CDS	291..5684
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062431.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062431.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACACTGTAGA CTCTACTCCA CTTCCAGTTC ATACACCAAC CTTAAGGAAA 
AAGGGATGCC CTGGCTCAAC TGGCTTTCCA TCGAAGCGCA AGATTCATCA GTTTTTTGTC 
AAATCTTTTA CTACTCCCAC CAGGTGTCAT CAGTGTACAT CTTTGATGGT GGGTTTGATA 
AGACAAGGCT GTTCTTGTGA AGTGTGTGGA TTTTCATGTC ACATAACTTG TGTAAACAAA 
GCTCCAACTG CTTGTCCAGT TCCTCCTGAA CAGACAAAAG GTCCTCTGGG TATAGATCCA 
CAGAAAGGAA TAGGAACAGC ATATGAAGGG CATGTCAGGA TCCCTAAGCC AGCTGGAGTG 
AAGAAAGGAT GGCAAAGAGC ACTGGCTGTA GTCTGTGACT TCAAGCTCTT TCTGTACGAT 
ATTGCCGAAG GAAAGGCATC TCAGCCCAGT GTTGTAGTCA GTCAAGTGAT TGACATGAGG 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTG GCTTCTGATG TTATTCATGC AAGTCGAAAA 
GATATACCCT GTATATTTAG AGTCACAGCT TCCCAGCTCT CAGCATCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCTGA TAGTGAAAAT GAGAAGAGTA AATGGGTGGG AGTACTGAGT 
GAATTGCACA AGATTTTGAA GAAAAACAAA TTCAGAGACC GCTCAGTCTA TGTTCCCAAA 
GAGGCTTATG ACAGCACTCT GCCCCTCATT AAAACAACCC AGGCAGCTGC AATCATAGAT 
CATGAAAGAA TAGCTTTGGG GAATGAAGAA GGGTTATTTG TTGTGCATGT CACCAAAGAT 
GAAATTATTA GAGTTGGTGA CAATAAGAAG ATTCATCAGA TAGAACTCAT TCCAAATGAT 
CAACTTGTTG CCGTGATCTC AGGACGAAAT CGACACGTCC GACTTTTTCC TATGTCAGCT 
CTGGACGGGC GAGAGACTGA TTTTTATAAG CTGGCAGAAA CTAAAGGCTG TCAAACAATA 
ACTTCTGGCA AAGTGCGTCA TGGAGCTCTT ACATGCTTGT GTGTGGCTAT GAAAAGGCAG 
GTCCTCTGTT ATGAACTATT TCAGAGCAAG ACGCGTCACA GAAAATTTAA GGAAATTCAA 
GTCCCGTACA ACGTCCAGTG GATGGCGATC TTCAGTGAGC AGCTCTGTGT GGGATTCCAG 
TCTGGATTTC TAAGATATCC CCTGAATGGA GAAGGAAGTC CATACAGTAT GCTCCATTCA 
AATGACAATA CACTCTCATT TATTGCACAT CAACCAATGG ATGCCATCTG TGCGGTTGAA 
ATCTCCAGTA AAGAATACCT GCTGTGTTTT AACAGCATTG GAATATACAC TGACTGCCAG 
GGCCGAAGAT CTAGACAACA GGAACTGATG TGGCCAGCGA ATCCTTCCTC TTGTTGTTAC 
AATGCACCGT ATCTCTCAGT GTACAGTGAA AACGCAGTTG ACATCTTTGA TGTGAACTCC 
ATGGAATGGA TTCAGACTCT CCCACTCAAA AAGGTTCGCC CCTTAAACAG TGAAGGATCA 
TTAAATCTTT TAGGCTTGGA GACGATTAGA TTGATATATT TCAAAAATAA AATGGCAGAA 
GGGGATGAAC TGGTAGTTCC TGAAACATCG GATAATAGCC GGAAGCAAAT GGTTAGAAAT 
ATTAACAATA AGAGGCGTTA CTCTTTCAGA GTTCCGGAAG AGGAAAGAAT GCAACAGAGG 
AGGGAGATGT TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACATATGGG TCCAGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
CCGGGTTTCC CTTATCCATC CCCTCATCAT CACTCCGGTC TGATCTCCTC TCCGATCAAC 
TTTGAGCACA TCTATCACAT GACTGTTAAT TCTGCTGAAA AATTTCTCTC TCCTGATTCC 
ATAAACCCTG AATATTCCCC GTCTCTGCGC TCTGTCCCTG GTACACCAAG CTTTATGACT 
CTGAGGAACC CTCGGCCTCA GGAAAGTCGG ACAGCATTCA GTGGCTCGGT CAGTATTCCG 
TCTATCACCA AATCCCGCCC TGAACCCGGT CGCTCCATGA GCGCCAGCAG TGGCCTATCT 
GCAAGGTCAT CTGCACAGAA TGGCAGTGCG TTAAAGAGAG AATTCTCTGG AGGCAGCTAC 
AGTGCAAAGC GGCAGCCCAT GCCCTCCCCA TCGGAGGGCT CGCTGTCCTC AGGAGGCATG 
GACCAGGGAA GTGACGCCCC AGCGCGGGAC TATGACGGAG AGGACTCTGA TTCTCCGCGG 
CATTCCACGG CTTCCAACAG TTCCAACCTG AGCAGTCCTC CCAGCCCGGT GTCGCCCCGG 
AAGACCAAGA GCCTGTCTCT GGAGAGCACA GACCGCGGGA GCTGGGACCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062431.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062493.1 Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X6, mRNA.

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CloneID	OBu18508
Clone ID Related Accession (Same CDS sequence)	XM_006062431.1 , XM_006062431.2
Accession Version	XM_006062431.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006062431.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACACTGTAGA CTCTACTCCA CTTCCAGTTC ATACACCAAC CTTAAGGAAA 
AAGGGATGCC CTGGCTCAAC TGGCTTTCCA TCGAAGCGCA AGATTCATCA GTTTTTTGTC 
AAATCTTTTA CTACTCCCAC CAGGTGTCAT CAGTGTACAT CTTTGATGGT GGGTTTGATA 
AGACAAGGCT GTTCTTGTGA AGTGTGTGGA TTTTCATGTC ACATAACTTG TGTAAACAAA 
GCTCCAACTG CTTGTCCAGT TCCTCCTGAA CAGACAAAAG GTCCTCTGGG TATAGATCCA 
CAGAAAGGAA TAGGAACAGC ATATGAAGGG CATGTCAGGA TCCCTAAGCC AGCTGGAGTG 
AAGAAAGGAT GGCAAAGAGC ACTGGCTGTA GTCTGTGACT TCAAGCTCTT TCTGTACGAT 
ATTGCCGAAG GAAAGGCATC TCAGCCCAGT GTTGTAGTCA GTCAAGTGAT TGACATGAGG 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTG GCTTCTGATG TTATTCATGC AAGTCGAAAA 
GATATACCCT GTATATTTAG AGTCACAGCT TCCCAGCTCT CAGCATCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCTGA TAGTGAAAAT GAGAAGAGTA AATGGGTGGG AGTACTGAGT 
GAATTGCACA AGATTTTGAA GAAAAACAAA TTCAGAGACC GCTCAGTCTA TGTTCCCAAA 
GAGGCTTATG ACAGCACTCT GCCCCTCATT AAAACAACCC AGGCAGCTGC AATCATAGAT 
CATGAAAGAA TAGCTTTGGG GAATGAAGAA GGGTTATTTG TTGTGCATGT CACCAAAGAT 
GAAATTATTA GAGTTGGTGA CAATAAGAAG ATTCATCAGA TAGAACTCAT TCCAAATGAT 
CAACTTGTTG CCGTGATCTC AGGACGAAAT CGACACGTCC GACTTTTTCC TATGTCAGCT 
CTGGACGGGC GAGAGACTGA TTTTTATAAG CTGGCAGAAA CTAAAGGCTG TCAAACAATA 
ACTTCTGGCA AAGTGCGTCA TGGAGCTCTT ACATGCTTGT GTGTGGCTAT GAAAAGGCAG 
GTCCTCTGTT ATGAACTATT TCAGAGCAAG ACGCGTCACA GAAAATTTAA GGAAATTCAA 
GTCCCGTACA ACGTCCAGTG GATGGCGATC TTCAGTGAGC AGCTCTGTGT GGGATTCCAG 
TCTGGATTTC TAAGATATCC CCTGAATGGA GAAGGAAGTC CATACAGTAT GCTCCATTCA 
AATGACAATA CACTCTCATT TATTGCACAT CAACCAATGG ATGCCATCTG TGCGGTTGAA 
ATCTCCAGTA AAGAATACCT GCTGTGTTTT AACAGCATTG GAATATACAC TGACTGCCAG 
GGCCGAAGAT CTAGACAACA GGAACTGATG TGGCCAGCGA ATCCTTCCTC TTGTTGTTAC 
AATGCACCGT ATCTCTCAGT GTACAGTGAA AACGCAGTTG ACATCTTTGA TGTGAACTCC 
ATGGAATGGA TTCAGACTCT CCCACTCAAA AAGGTTCGCC CCTTAAACAG TGAAGGATCA 
TTAAATCTTT TAGGCTTGGA GACGATTAGA TTGATATATT TCAAAAATAA AATGGCAGAA 
GGGGATGAAC TGGTAGTTCC TGAAACATCG GATAATAGCC GGAAGCAAAT GGTTAGAAAT 
ATTAACAATA AGAGGCGTTA CTCTTTCAGA GTTCCGGAAG AGGAAAGAAT GCAACAGAGG 
AGGGAGATGT TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACATATGGG TCCAGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
CCGGGTTTCC CTTATCCATC CCCTCATCAT CACTCCGGTC TGATCTCCTC TCCGATCAAC 
TTTGAGCACA TCTATCACAT GACTGTTAAT TCTGCTGAAA AATTTCTCTC TCCTGATTCC 
ATAAACCCTG AATATTCCCC GTCTCTGCGC TCTGTCCCTG GTACACCAAG CTTTATGACT 
CTGAGGAACC CTCGGCCTCA GGAAAGTCGG ACAGCATTCA GTGGCTCGGT CAGTATTCCG 
TCTATCACCA AATCCCGCCC TGAACCCGGT CGCTCCATGA GCGCCAGCAG TGGCCTATCT 
GCAAGGTCAT CTGCACAGAA TGGCAGTGCG TTAAAGAGAG AATTCTCTGG AGGCAGCTAC 
AGTGCAAAGC GGCAGCCCAT GCCCTCCCCA TCGGAGGGCT CGCTGTCCTC AGGAGGCATG 
GACCAGGGAA GTGACGCCCC AGCGCGGGAC TATGACGGAG AGGACTCTGA TTCTCCGCGG 
CATTCCACGG CTTCCAACAG TTCCAACCTG AGCAGTCCTC CCAGCCCGGT GTCGCCCCGG 
AAGACCAAGA GCCTGTCTCT GGAGAGCACA GACCGCGGGA GCTGGGACCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062493.1
CDS	766..6159
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062431.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062431.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACACTGTAGA CTCTACTCCA CTTCCAGTTC ATACACCAAC CTTAAGGAAA 
AAGGGATGCC CTGGCTCAAC TGGCTTTCCA TCGAAGCGCA AGATTCATCA GTTTTTTGTC 
AAATCTTTTA CTACTCCCAC CAGGTGTCAT CAGTGTACAT CTTTGATGGT GGGTTTGATA 
AGACAAGGCT GTTCTTGTGA AGTGTGTGGA TTTTCATGTC ACATAACTTG TGTAAACAAA 
GCTCCAACTG CTTGTCCAGT TCCTCCTGAA CAGACAAAAG GTCCTCTGGG TATAGATCCA 
CAGAAAGGAA TAGGAACAGC ATATGAAGGG CATGTCAGGA TCCCTAAGCC AGCTGGAGTG 
AAGAAAGGAT GGCAAAGAGC ACTGGCTGTA GTCTGTGACT TCAAGCTCTT TCTGTACGAT 
ATTGCCGAAG GAAAGGCATC TCAGCCCAGT GTTGTAGTCA GTCAAGTGAT TGACATGAGG 
GATGAAGAAT TTTCTGTGAG TTCAGTCTTG GCTTCTGATG TTATTCATGC AAGTCGAAAA 
GATATACCCT GTATATTTAG AGTCACAGCT TCCCAGCTCT CAGCATCTAG TAACAAATGT 
TCCATCCTGA TGCTAGCTGA TAGTGAAAAT GAGAAGAGTA AATGGGTGGG AGTACTGAGT 
GAATTGCACA AGATTTTGAA GAAAAACAAA TTCAGAGACC GCTCAGTCTA TGTTCCCAAA 
GAGGCTTATG ACAGCACTCT GCCCCTCATT AAAACAACCC AGGCAGCTGC AATCATAGAT 
CATGAAAGAA TAGCTTTGGG GAATGAAGAA GGGTTATTTG TTGTGCATGT CACCAAAGAT 
GAAATTATTA GAGTTGGTGA CAATAAGAAG ATTCATCAGA TAGAACTCAT TCCAAATGAT 
CAACTTGTTG CCGTGATCTC AGGACGAAAT CGACACGTCC GACTTTTTCC TATGTCAGCT 
CTGGACGGGC GAGAGACTGA TTTTTATAAG CTGGCAGAAA CTAAAGGCTG TCAAACAATA 
ACTTCTGGCA AAGTGCGTCA TGGAGCTCTT ACATGCTTGT GTGTGGCTAT GAAAAGGCAG 
GTCCTCTGTT ATGAACTATT TCAGAGCAAG ACGCGTCACA GAAAATTTAA GGAAATTCAA 
GTCCCGTACA ACGTCCAGTG GATGGCGATC TTCAGTGAGC AGCTCTGTGT GGGATTCCAG 
TCTGGATTTC TAAGATATCC CCTGAATGGA GAAGGAAGTC CATACAGTAT GCTCCATTCA 
AATGACAATA CACTCTCATT TATTGCACAT CAACCAATGG ATGCCATCTG TGCGGTTGAA 
ATCTCCAGTA AAGAATACCT GCTGTGTTTT AACAGCATTG GAATATACAC TGACTGCCAG 
GGCCGAAGAT CTAGACAACA GGAACTGATG TGGCCAGCGA ATCCTTCCTC TTGTTGTTAC 
AATGCACCGT ATCTCTCAGT GTACAGTGAA AACGCAGTTG ACATCTTTGA TGTGAACTCC 
ATGGAATGGA TTCAGACTCT CCCACTCAAA AAGGTTCGCC CCTTAAACAG TGAAGGATCA 
TTAAATCTTT TAGGCTTGGA GACGATTAGA TTGATATATT TCAAAAATAA AATGGCAGAA 
GGGGATGAAC TGGTAGTTCC TGAAACATCG GATAATAGCC GGAAGCAAAT GGTTAGAAAT 
ATTAACAATA AGAGGCGTTA CTCTTTCAGA GTTCCGGAAG AGGAAAGAAT GCAACAGAGG 
AGGGAGATGT TACGAGATCC AGAAATGAGA AATAAATTAA TTTCTAACCC AACTAATTTT 
AACCACATAG CACATATGGG TCCAGGAGAT GGAATACAGA TCCTAAAAGA CCTGCCCATG 
CCGGGTTTCC CTTATCCATC CCCTCATCAT CACTCCGGTC TGATCTCCTC TCCGATCAAC 
TTTGAGCACA TCTATCACAT GACTGTTAAT TCTGCTGAAA AATTTCTCTC TCCTGATTCC 
ATAAACCCTG AATATTCCCC GTCTCTGCGC TCTGTCCCTG GTACACCAAG CTTTATGACT 
CTGAGGAACC CTCGGCCTCA GGAAAGTCGG ACAGCATTCA GTGGCTCGGT CAGTATTCCG 
TCTATCACCA AATCCCGCCC TGAACCCGGT CGCTCCATGA GCGCCAGCAG TGGCCTATCT 
GCAAGGTCAT CTGCACAGAA TGGCAGTGCG TTAAAGAGAG AATTCTCTGG AGGCAGCTAC 
AGTGCAAAGC GGCAGCCCAT GCCCTCCCCA TCGGAGGGCT CGCTGTCCTC AGGAGGCATG 
GACCAGGGAA GTGACGCCCC AGCGCGGGAC TATGACGGAG AGGACTCTGA TTCTCCGCGG 
CATTCCACGG CTTCCAACAG TTCCAACCTG AGCAGTCCTC CCAGCCCGGT GTCGCCCCGG 
AAGACCAAGA GCCTGTCTCT GGAGAGCACA GACCGCGGGA GCTGGGACCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062432.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062494.1 Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X7, mRNA.

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CloneID	OBu18509
Clone ID Related Accession (Same CDS sequence)	XM_006062432.1
Accession Version	XM_006062432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTACTGAT CCCGTTGAGA ACACATATGT ACGGAATCCG 
AACGTCAAGT TTTACATCCA GTCAAGGTCC ACCTCTCCTT CCACATCTAG TGAAGCTGAG 
CCAGTTAAGA CTGTAGACTC TACTCCACTT CCAGTTCATA CACCAACCTT AAGGAAAAAG 
GGATGCCCTG GCTCAACTGG CTTTCCATCG AAGCGCAAGA TTCATCAGTT TTTTGTCAAA 
TCTTTTACTA CTCCCACCAG GTGTCATCAG TGTACATCTT TGATGGTGGG TTTGATAAGA 
CAAGGCTGTT CTTGTGAAGT GTGTGGATTT TCATGTCACA TAACTTGTGT AAACAAAGCT 
CCAACTGCTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CTCTGGGTAT AGATCCACAG 
AAAGGAATAG GAACAGCATA TGAAGGGCAT GTCAGGATCC CTAAGCCAGC TGGAGTGAAG 
AAAGGATGGC AAAGAGCACT GGCTGTAGTC TGTGACTTCA AGCTCTTTCT GTACGATATT 
GCCGAAGGAA AGGCATCTCA GCCCAGTGTT GTAGTCAGTC AAGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TTCATGCAAG TCGAAAAGAT 
ATACCCTGTA TATTTAGAGT CACAGCTTCC CAGCTCTCAG CATCTAGTAA CAAATGTTCC 
ATCCTGATGC TAGCTGATAG TGAAAATGAG AAGAGTAAAT GGGTGGGAGT ACTGAGTGAA 
TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG 
GCTTATGACA GCACTCTGCC CCTCATTAAA ACAACCCAGG CAGCTGCAAT CATAGATCAT 
GAAAGAATAG CTTTGGGGAA TGAAGAAGGG TTATTTGTTG TGCATGTCAC CAAAGATGAA 
ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATAG AACTCATTCC AAATGATCAA 
CTTGTTGCCG TGATCTCAGG ACGAAATCGA CACGTCCGAC TTTTTCCTAT GTCAGCTCTG 
GACGGGCGAG AGACTGATTT TTATAAGCTG GCAGAAACTA AAGGCTGTCA AACAATAACT 
TCTGGCAAAG TGCGTCATGG AGCTCTTACA TGCTTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACG CGTCACAGAA AATTTAAGGA AATTCAAGTC 
CCGTACAACG TCCAGTGGAT GGCGATCTTC AGTGAGCAGC TCTGTGTGGG ATTCCAGTCT 
GGATTTCTAA GATATCCCCT GAATGGAGAA GGAAGTCCAT ACAGTATGCT CCATTCAAAT 
GACAATACAC TCTCATTTAT TGCACATCAA CCAATGGATG CCATCTGTGC GGTTGAAATC 
TCCAGTAAAG AATACCTGCT GTGTTTTAAC AGCATTGGAA TATACACTGA CTGCCAGGGC 
CGAAGATCTA GACAACAGGA ACTGATGTGG CCAGCGAATC CTTCCTCTTG TTGTTACAAT 
GCACCGTATC TCTCAGTGTA CAGTGAAAAC GCAGTTGACA TCTTTGATGT GAACTCCATG 
GAATGGATTC AGACTCTCCC ACTCAAAAAG GTTCGCCCCT TAAACAGTGA AGGATCATTA 
AATCTTTTAG GCTTGGAGAC GATTAGATTG ATATATTTCA AAAATAAAAT GGCAGAAGGG 
GATGAACTGG TAGTTCCTGA AACATCGGAT AATAGCCGGA AGCAAATGGT TAGAAATATT 
AACAATAAGA GGCGTTACTC TTTCAGAGTT CCGGAAGAGG AAAGAATGCA ACAGAGGAGG 
GAGATGTTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAACCCAAC TAATTTTAAC 
CACATAGCAC ATATGGGTCC AGGAGATGGA ATACAGATCC TAAAAGACCT GCCCATGCCG 
GGTTTCCCTT ATCCATCCCC TCATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT 
GAGCACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA 
AACCCTGAAT ATTCCCCGTC TCTGCGCTCT GTCCCTGGTA CACCAAGCTT TATGACTCTG 
AGGAACCCTC GGCCTCAGGA AAGTCGGACA GCATTCAGTG GCTCGGTCAG TATTCCGTCT 
ATCACCAAAT CCCGCCCTGA ACCCGGTCGC TCCATGAGCG CCAGCAGTGG CCTATCTGCA 
AGGTCATCTG CACAGAATGG CAGTGCGTTA AAGAGAGAAT TCTCTGGAGG CAGCTACAGT 
GCAAAGCGGC AGCCCATGCC CTCCCCATCG GAGGGCTCGC TGTCCTCAGG AGGCATGGAC 
CAGGGAAGTG ACGCCCCAGC GCGGGACTAT GACGGAGAGG ACTCTGATTC TCCGCGGCAT 
TCCACGGCTT CCAACAGTTC CAACCTGAGC AGTCCTCCCA GCCCGGTGTC GCCCCGGAAG 
ACCAAGAGCC TGTCTCTGGA GAGCACAGAC CGCGGGAGCT GGGACCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062494.1
CDS	1075..6465
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKASKGRRTDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062432.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062432.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTACTGAT CCCGTTGAGA ACACATATGT ACGGAATCCG 
AACGTCAAGT TTTACATCCA GTCAAGGTCC ACCTCTCCTT CCACATCTAG TGAAGCTGAG 
CCAGTTAAGA CTGTAGACTC TACTCCACTT CCAGTTCATA CACCAACCTT AAGGAAAAAG 
GGATGCCCTG GCTCAACTGG CTTTCCATCG AAGCGCAAGA TTCATCAGTT TTTTGTCAAA 
TCTTTTACTA CTCCCACCAG GTGTCATCAG TGTACATCTT TGATGGTGGG TTTGATAAGA 
CAAGGCTGTT CTTGTGAAGT GTGTGGATTT TCATGTCACA TAACTTGTGT AAACAAAGCT 
CCAACTGCTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CTCTGGGTAT AGATCCACAG 
AAAGGAATAG GAACAGCATA TGAAGGGCAT GTCAGGATCC CTAAGCCAGC TGGAGTGAAG 
AAAGGATGGC AAAGAGCACT GGCTGTAGTC TGTGACTTCA AGCTCTTTCT GTACGATATT 
GCCGAAGGAA AGGCATCTCA GCCCAGTGTT GTAGTCAGTC AAGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TTCATGCAAG TCGAAAAGAT 
ATACCCTGTA TATTTAGAGT CACAGCTTCC CAGCTCTCAG CATCTAGTAA CAAATGTTCC 
ATCCTGATGC TAGCTGATAG TGAAAATGAG AAGAGTAAAT GGGTGGGAGT ACTGAGTGAA 
TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG 
GCTTATGACA GCACTCTGCC CCTCATTAAA ACAACCCAGG CAGCTGCAAT CATAGATCAT 
GAAAGAATAG CTTTGGGGAA TGAAGAAGGG TTATTTGTTG TGCATGTCAC CAAAGATGAA 
ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATAG AACTCATTCC AAATGATCAA 
CTTGTTGCCG TGATCTCAGG ACGAAATCGA CACGTCCGAC TTTTTCCTAT GTCAGCTCTG 
GACGGGCGAG AGACTGATTT TTATAAGCTG GCAGAAACTA AAGGCTGTCA AACAATAACT 
TCTGGCAAAG TGCGTCATGG AGCTCTTACA TGCTTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACG CGTCACAGAA AATTTAAGGA AATTCAAGTC 
CCGTACAACG TCCAGTGGAT GGCGATCTTC AGTGAGCAGC TCTGTGTGGG ATTCCAGTCT 
GGATTTCTAA GATATCCCCT GAATGGAGAA GGAAGTCCAT ACAGTATGCT CCATTCAAAT 
GACAATACAC TCTCATTTAT TGCACATCAA CCAATGGATG CCATCTGTGC GGTTGAAATC 
TCCAGTAAAG AATACCTGCT GTGTTTTAAC AGCATTGGAA TATACACTGA CTGCCAGGGC 
CGAAGATCTA GACAACAGGA ACTGATGTGG CCAGCGAATC CTTCCTCTTG TTGTTACAAT 
GCACCGTATC TCTCAGTGTA CAGTGAAAAC GCAGTTGACA TCTTTGATGT GAACTCCATG 
GAATGGATTC AGACTCTCCC ACTCAAAAAG GTTCGCCCCT TAAACAGTGA AGGATCATTA 
AATCTTTTAG GCTTGGAGAC GATTAGATTG ATATATTTCA AAAATAAAAT GGCAGAAGGG 
GATGAACTGG TAGTTCCTGA AACATCGGAT AATAGCCGGA AGCAAATGGT TAGAAATATT 
AACAATAAGA GGCGTTACTC TTTCAGAGTT CCGGAAGAGG AAAGAATGCA ACAGAGGAGG 
GAGATGTTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAACCCAAC TAATTTTAAC 
CACATAGCAC ATATGGGTCC AGGAGATGGA ATACAGATCC TAAAAGACCT GCCCATGCCG 
GGTTTCCCTT ATCCATCCCC TCATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT 
GAGCACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA 
AACCCTGAAT ATTCCCCGTC TCTGCGCTCT GTCCCTGGTA CACCAAGCTT TATGACTCTG 
AGGAACCCTC GGCCTCAGGA AAGTCGGACA GCATTCAGTG GCTCGGTCAG TATTCCGTCT 
ATCACCAAAT CCCGCCCTGA ACCCGGTCGC TCCATGAGCG CCAGCAGTGG CCTATCTGCA 
AGGTCATCTG CACAGAATGG CAGTGCGTTA AAGAGAGAAT TCTCTGGAGG CAGCTACAGT 
GCAAAGCGGC AGCCCATGCC CTCCCCATCG GAGGGCTCGC TGTCCTCAGG AGGCATGGAC 
CAGGGAAGTG ACGCCCCAGC GCGGGACTAT GACGGAGAGG ACTCTGATTC TCCGCGGCAT 
TCCACGGCTT CCAACAGTTC CAACCTGAGC AGTCCTCCCA GCCCGGTGTC GCCCCGGAAG 
ACCAAGAGCC TGTCTCTGGA GAGCACAGAC CGCGGGAGCT GGGACCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062433.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062495.1 Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X7, mRNA.

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CloneID	OBu18510
Clone ID Related Accession (Same CDS sequence)	XM_006062433.2 , XM_006062433.1
Accession Version	XM_006062433.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006062433.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCTACTCC ACTTCCAGTT CATACACCAA CCTTAAGGAA AAAGGGATGC 
CCTGGCTCAA CTGGCTTTCC ATCGAAGCGC AAGATTCATC AGTTTTTTGT CAAATCTTTT 
ACTACTCCCA CCAGGTGTCA TCAGTGTACA TCTTTGATGG TGGGTTTGAT AAGACAAGGC 
TGTTCTTGTG AAGTGTGTGG ATTTTCATGT CACATAACTT GTGTAAACAA AGCTCCAACT 
GCTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCTCTGG GTATAGATCC ACAGAAAGGA 
ATAGGAACAG CATATGAAGG GCATGTCAGG ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA 
TGGCAAAGAG CACTGGCTGT AGTCTGTGAC TTCAAGCTCT TTCTGTACGA TATTGCCGAA 
GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC 
TGTATATTTA GAGTCACAGC TTCCCAGCTC TCAGCATCTA GTAACAAATG TTCCATCCTG 
ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT AAATGGGTGG GAGTACTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCATGAAAGA 
ATAGCTTTGG GGAATGAAGA AGGGTTATTT GTTGTGCATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATAGAACTCA TTCCAAATGA TCAACTTGTT 
GCCGTGATCT CAGGACGAAA TCGACACGTC CGACTTTTTC CTATGTCAGC TCTGGACGGG 
CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGCT GTCAAACAAT AACTTCTGGC 
AAAGTGCGTC ATGGAGCTCT TACATGCTTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACGCGTCAC AGAAAATTTA AGGAAATTCA AGTCCCGTAC 
AACGTCCAGT GGATGGCGAT CTTCAGTGAG CAGCTCTGTG TGGGATTCCA GTCTGGATTT 
CTAAGATATC CCCTGAATGG AGAAGGAAGT CCATACAGTA TGCTCCATTC AAATGACAAT 
ACACTCTCAT TTATTGCACA TCAACCAATG GATGCCATCT GTGCGGTTGA AATCTCCAGT 
AAAGAATACC TGCTGTGTTT TAACAGCATT GGAATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAACTGAT GTGGCCAGCG AATCCTTCCT CTTGTTGTTA CAATGCACCG 
TATCTCTCAG TGTACAGTGA AAACGCAGTT GACATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TCCCACTCAA AAAGGTTCGC CCCTTAAACA GTGAAGGATC ATTAAATCTT 
TTAGGCTTGG AGACGATTAG ATTGATATAT TTCAAAAATA AAATGGCAGA AGGGGATGAA 
CTGGTAGTTC CTGAAACATC GGATAATAGC CGGAAGCAAA TGGTTAGAAA TATTAACAAT 
AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA GAGGAAAGAA TGCAACAGAG GAGGGAGATG 
TTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACATATGG GTCCAGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GCCGGGTTTC 
CCTTATCCAT CCCCTCATCA TCACTCCGGT CTGATCTCCT CTCCGATCAA CTTTGAGCAC 
ATCTATCACA TGACTGTTAA TTCTGCTGAA AAATTTCTCT CTCCTGATTC CATAAACCCT 
GAATATTCCC CGTCTCTGCG CTCTGTCCCT GGTACACCAA GCTTTATGAC TCTGAGGAAC 
CCTCGGCCTC AGGAAAGTCG GACAGCATTC AGTGGCTCGG TCAGTATTCC GTCTATCACC 
AAATCCCGCC CTGAACCCGG TCGCTCCATG AGCGCCAGCA GTGGCCTATC TGCAAGGTCA 
TCTGCACAGA ATGGCAGTGC GTTAAAGAGA GAATTCTCTG GAGGCAGCTA CAGTGCAAAG 
CGGCAGCCCA TGCCCTCCCC ATCGGAGGGC TCGCTGTCCT CAGGAGGCAT GGACCAGGGA 
AGTGACGCCC CAGCGCGGGA CTATGACGGA GAGGACTCTG ATTCTCCGCG GCATTCCACG 
GCTTCCAACA GTTCCAACCT GAGCAGTCCT CCCAGCCCGG TGTCGCCCCG GAAGACCAAG 
AGCCTGTCTC TGGAGAGCAC AGACCGCGGG AGCTGGGACC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062495.1
CDS	766..6150
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062433.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062433.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCTACTCC ACTTCCAGTT CATACACCAA CCTTAAGGAA AAAGGGATGC 
CCTGGCTCAA CTGGCTTTCC ATCGAAGCGC AAGATTCATC AGTTTTTTGT CAAATCTTTT 
ACTACTCCCA CCAGGTGTCA TCAGTGTACA TCTTTGATGG TGGGTTTGAT AAGACAAGGC 
TGTTCTTGTG AAGTGTGTGG ATTTTCATGT CACATAACTT GTGTAAACAA AGCTCCAACT 
GCTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCTCTGG GTATAGATCC ACAGAAAGGA 
ATAGGAACAG CATATGAAGG GCATGTCAGG ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA 
TGGCAAAGAG CACTGGCTGT AGTCTGTGAC TTCAAGCTCT TTCTGTACGA TATTGCCGAA 
GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC 
TGTATATTTA GAGTCACAGC TTCCCAGCTC TCAGCATCTA GTAACAAATG TTCCATCCTG 
ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT AAATGGGTGG GAGTACTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCATGAAAGA 
ATAGCTTTGG GGAATGAAGA AGGGTTATTT GTTGTGCATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATAGAACTCA TTCCAAATGA TCAACTTGTT 
GCCGTGATCT CAGGACGAAA TCGACACGTC CGACTTTTTC CTATGTCAGC TCTGGACGGG 
CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGCT GTCAAACAAT AACTTCTGGC 
AAAGTGCGTC ATGGAGCTCT TACATGCTTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACGCGTCAC AGAAAATTTA AGGAAATTCA AGTCCCGTAC 
AACGTCCAGT GGATGGCGAT CTTCAGTGAG CAGCTCTGTG TGGGATTCCA GTCTGGATTT 
CTAAGATATC CCCTGAATGG AGAAGGAAGT CCATACAGTA TGCTCCATTC AAATGACAAT 
ACACTCTCAT TTATTGCACA TCAACCAATG GATGCCATCT GTGCGGTTGA AATCTCCAGT 
AAAGAATACC TGCTGTGTTT TAACAGCATT GGAATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAACTGAT GTGGCCAGCG AATCCTTCCT CTTGTTGTTA CAATGCACCG 
TATCTCTCAG TGTACAGTGA AAACGCAGTT GACATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TCCCACTCAA AAAGGTTCGC CCCTTAAACA GTGAAGGATC ATTAAATCTT 
TTAGGCTTGG AGACGATTAG ATTGATATAT TTCAAAAATA AAATGGCAGA AGGGGATGAA 
CTGGTAGTTC CTGAAACATC GGATAATAGC CGGAAGCAAA TGGTTAGAAA TATTAACAAT 
AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA GAGGAAAGAA TGCAACAGAG GAGGGAGATG 
TTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACATATGG GTCCAGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GCCGGGTTTC 
CCTTATCCAT CCCCTCATCA TCACTCCGGT CTGATCTCCT CTCCGATCAA CTTTGAGCAC 
ATCTATCACA TGACTGTTAA TTCTGCTGAA AAATTTCTCT CTCCTGATTC CATAAACCCT 
GAATATTCCC CGTCTCTGCG CTCTGTCCCT GGTACACCAA GCTTTATGAC TCTGAGGAAC 
CCTCGGCCTC AGGAAAGTCG GACAGCATTC AGTGGCTCGG TCAGTATTCC GTCTATCACC 
AAATCCCGCC CTGAACCCGG TCGCTCCATG AGCGCCAGCA GTGGCCTATC TGCAAGGTCA 
TCTGCACAGA ATGGCAGTGC GTTAAAGAGA GAATTCTCTG GAGGCAGCTA CAGTGCAAAG 
CGGCAGCCCA TGCCCTCCCC ATCGGAGGGC TCGCTGTCCT CAGGAGGCAT GGACCAGGGA 
AGTGACGCCC CAGCGCGGGA CTATGACGGA GAGGACTCTG ATTCTCCGCG GCATTCCACG 
GCTTCCAACA GTTCCAACCT GAGCAGTCCT CCCAGCCCGG TGTCGCCCCG GAAGACCAAG 
AGCCTGTCTC TGGAGAGCAC AGACCGCGGG AGCTGGGACC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062433.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062495.1 Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X8, mRNA.

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CloneID	OBu18510
Clone ID Related Accession (Same CDS sequence)	XM_006062433.2 , XM_006062433.1
Accession Version	XM_006062433.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCTACTCC ACTTCCAGTT CATACACCAA CCTTAAGGAA AAAGGGATGC 
CCTGGCTCAA CTGGCTTTCC ATCGAAGCGC AAGATTCATC AGTTTTTTGT CAAATCTTTT 
ACTACTCCCA CCAGGTGTCA TCAGTGTACA TCTTTGATGG TGGGTTTGAT AAGACAAGGC 
TGTTCTTGTG AAGTGTGTGG ATTTTCATGT CACATAACTT GTGTAAACAA AGCTCCAACT 
GCTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCTCTGG GTATAGATCC ACAGAAAGGA 
ATAGGAACAG CATATGAAGG GCATGTCAGG ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA 
TGGCAAAGAG CACTGGCTGT AGTCTGTGAC TTCAAGCTCT TTCTGTACGA TATTGCCGAA 
GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC 
TGTATATTTA GAGTCACAGC TTCCCAGCTC TCAGCATCTA GTAACAAATG TTCCATCCTG 
ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT AAATGGGTGG GAGTACTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCATGAAAGA 
ATAGCTTTGG GGAATGAAGA AGGGTTATTT GTTGTGCATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATAGAACTCA TTCCAAATGA TCAACTTGTT 
GCCGTGATCT CAGGACGAAA TCGACACGTC CGACTTTTTC CTATGTCAGC TCTGGACGGG 
CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGCT GTCAAACAAT AACTTCTGGC 
AAAGTGCGTC ATGGAGCTCT TACATGCTTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACGCGTCAC AGAAAATTTA AGGAAATTCA AGTCCCGTAC 
AACGTCCAGT GGATGGCGAT CTTCAGTGAG CAGCTCTGTG TGGGATTCCA GTCTGGATTT 
CTAAGATATC CCCTGAATGG AGAAGGAAGT CCATACAGTA TGCTCCATTC AAATGACAAT 
ACACTCTCAT TTATTGCACA TCAACCAATG GATGCCATCT GTGCGGTTGA AATCTCCAGT 
AAAGAATACC TGCTGTGTTT TAACAGCATT GGAATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAACTGAT GTGGCCAGCG AATCCTTCCT CTTGTTGTTA CAATGCACCG 
TATCTCTCAG TGTACAGTGA AAACGCAGTT GACATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TCCCACTCAA AAAGGTTCGC CCCTTAAACA GTGAAGGATC ATTAAATCTT 
TTAGGCTTGG AGACGATTAG ATTGATATAT TTCAAAAATA AAATGGCAGA AGGGGATGAA 
CTGGTAGTTC CTGAAACATC GGATAATAGC CGGAAGCAAA TGGTTAGAAA TATTAACAAT 
AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA GAGGAAAGAA TGCAACAGAG GAGGGAGATG 
TTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACATATGG GTCCAGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GCCGGGTTTC 
CCTTATCCAT CCCCTCATCA TCACTCCGGT CTGATCTCCT CTCCGATCAA CTTTGAGCAC 
ATCTATCACA TGACTGTTAA TTCTGCTGAA AAATTTCTCT CTCCTGATTC CATAAACCCT 
GAATATTCCC CGTCTCTGCG CTCTGTCCCT GGTACACCAA GCTTTATGAC TCTGAGGAAC 
CCTCGGCCTC AGGAAAGTCG GACAGCATTC AGTGGCTCGG TCAGTATTCC GTCTATCACC 
AAATCCCGCC CTGAACCCGG TCGCTCCATG AGCGCCAGCA GTGGCCTATC TGCAAGGTCA 
TCTGCACAGA ATGGCAGTGC GTTAAAGAGA GAATTCTCTG GAGGCAGCTA CAGTGCAAAG 
CGGCAGCCCA TGCCCTCCCC ATCGGAGGGC TCGCTGTCCT CAGGAGGCAT GGACCAGGGA 
AGTGACGCCC CAGCGCGGGA CTATGACGGA GAGGACTCTG ATTCTCCGCG GCATTCCACG 
GCTTCCAACA GTTCCAACCT GAGCAGTCCT CCCAGCCCGG TGTCGCCCCG GAAGACCAAG 
AGCCTGTCTC TGGAGAGCAC AGACCGCGGG AGCTGGGACC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062495.1
CDS	290..5674
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062433.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062433.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCTACTCC ACTTCCAGTT CATACACCAA CCTTAAGGAA AAAGGGATGC 
CCTGGCTCAA CTGGCTTTCC ATCGAAGCGC AAGATTCATC AGTTTTTTGT CAAATCTTTT 
ACTACTCCCA CCAGGTGTCA TCAGTGTACA TCTTTGATGG TGGGTTTGAT AAGACAAGGC 
TGTTCTTGTG AAGTGTGTGG ATTTTCATGT CACATAACTT GTGTAAACAA AGCTCCAACT 
GCTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCTCTGG GTATAGATCC ACAGAAAGGA 
ATAGGAACAG CATATGAAGG GCATGTCAGG ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA 
TGGCAAAGAG CACTGGCTGT AGTCTGTGAC TTCAAGCTCT TTCTGTACGA TATTGCCGAA 
GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC 
TGTATATTTA GAGTCACAGC TTCCCAGCTC TCAGCATCTA GTAACAAATG TTCCATCCTG 
ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT AAATGGGTGG GAGTACTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCATGAAAGA 
ATAGCTTTGG GGAATGAAGA AGGGTTATTT GTTGTGCATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATAGAACTCA TTCCAAATGA TCAACTTGTT 
GCCGTGATCT CAGGACGAAA TCGACACGTC CGACTTTTTC CTATGTCAGC TCTGGACGGG 
CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGCT GTCAAACAAT AACTTCTGGC 
AAAGTGCGTC ATGGAGCTCT TACATGCTTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACGCGTCAC AGAAAATTTA AGGAAATTCA AGTCCCGTAC 
AACGTCCAGT GGATGGCGAT CTTCAGTGAG CAGCTCTGTG TGGGATTCCA GTCTGGATTT 
CTAAGATATC CCCTGAATGG AGAAGGAAGT CCATACAGTA TGCTCCATTC AAATGACAAT 
ACACTCTCAT TTATTGCACA TCAACCAATG GATGCCATCT GTGCGGTTGA AATCTCCAGT 
AAAGAATACC TGCTGTGTTT TAACAGCATT GGAATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAACTGAT GTGGCCAGCG AATCCTTCCT CTTGTTGTTA CAATGCACCG 
TATCTCTCAG TGTACAGTGA AAACGCAGTT GACATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TCCCACTCAA AAAGGTTCGC CCCTTAAACA GTGAAGGATC ATTAAATCTT 
TTAGGCTTGG AGACGATTAG ATTGATATAT TTCAAAAATA AAATGGCAGA AGGGGATGAA 
CTGGTAGTTC CTGAAACATC GGATAATAGC CGGAAGCAAA TGGTTAGAAA TATTAACAAT 
AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA GAGGAAAGAA TGCAACAGAG GAGGGAGATG 
TTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACATATGG GTCCAGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GCCGGGTTTC 
CCTTATCCAT CCCCTCATCA TCACTCCGGT CTGATCTCCT CTCCGATCAA CTTTGAGCAC 
ATCTATCACA TGACTGTTAA TTCTGCTGAA AAATTTCTCT CTCCTGATTC CATAAACCCT 
GAATATTCCC CGTCTCTGCG CTCTGTCCCT GGTACACCAA GCTTTATGAC TCTGAGGAAC 
CCTCGGCCTC AGGAAAGTCG GACAGCATTC AGTGGCTCGG TCAGTATTCC GTCTATCACC 
AAATCCCGCC CTGAACCCGG TCGCTCCATG AGCGCCAGCA GTGGCCTATC TGCAAGGTCA 
TCTGCACAGA ATGGCAGTGC GTTAAAGAGA GAATTCTCTG GAGGCAGCTA CAGTGCAAAG 
CGGCAGCCCA TGCCCTCCCC ATCGGAGGGC TCGCTGTCCT CAGGAGGCAT GGACCAGGGA 
AGTGACGCCC CAGCGCGGGA CTATGACGGA GAGGACTCTG ATTCTCCGCG GCATTCCACG 
GCTTCCAACA GTTCCAACCT GAGCAGTCCT CCCAGCCCGG TGTCGCCCCG GAAGACCAAG 
AGCCTGTCTC TGGAGAGCAC AGACCGCGGG AGCTGGGACC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062434.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062496.1 Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X8, mRNA.

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CloneID	OBu18511
Clone ID Related Accession (Same CDS sequence)	XM_006062434.2 , XM_006062434.1
Accession Version	XM_006062434.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006062434.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGATCCC 
GTTGAGAACA CATATGTACG GAATCCGAAC GTCAAGTTTT ACATCCAGTC AAGGTCCACC 
TCTCCTTCCA CATCTAGTGA AGCTGAGCCA GTTAAGACTG TAGACTCTAC TCCACTTCCA 
GTTCATACAC CAACCTTAAG GAAAAAGGGA TGCCCTGGCT CAACTGGCTT TCCATCGAAG 
CGCAAGATTC ATCAGTTTTT TGTCAAATCT TTTACTACTC CCACCAGGTG TCATCAGTGT 
ACATCTTTGA TGGTGGGTTT GATAAGACAA GGCTGTTCTT GTGAAGTGTG TGGATTTTCA 
TGTCACATAA CTTGTGTAAA CAAAGCTCCA ACTGCTTGTC CAGTTCCTCC TGAACAGACA 
AAAGGTCCTC TGGGTATAGA TCCACAGAAA GGAATAGGAA CAGCATATGA AGGGCATGTC 
AGGATCCCTA AGCCAGCTGG AGTGAAGAAA GGATGGCAAA GAGCACTGGC TGTAGTCTGT 
GACTTCAAGC TCTTTCTGTA CGATATTGCC GAAGGAAAGG CATCTCAGCC CAGTGTTGTA 
GTCAGTCAAG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT CTTGGCTTCT 
GATGTTATTC ATGCAAGTCG AAAAGATATA CCCTGTATAT TTAGAGTCAC AGCTTCCCAG 
CTCTCAGCAT CTAGTAACAA ATGTTCCATC CTGATGCTAG CTGATAGTGA AAATGAGAAG 
AGTAAATGGG TGGGAGTACT GAGTGAATTG CACAAGATTT TGAAGAAAAA CAAATTCAGA 
GACCGCTCAG TCTATGTTCC CAAAGAGGCT TATGACAGCA CTCTGCCCCT CATTAAAACA 
ACCCAGGCAG CTGCAATCAT AGATCATGAA AGAATAGCTT TGGGGAATGA AGAAGGGTTA 
TTTGTTGTGC ATGTCACCAA AGATGAAATT ATTAGAGTTG GTGACAATAA GAAGATTCAT 
CAGATAGAAC TCATTCCAAA TGATCAACTT GTTGCCGTGA TCTCAGGACG AAATCGACAC 
GTCCGACTTT TTCCTATGTC AGCTCTGGAC GGGCGAGAGA CTGATTTTTA TAAGCTGGCA 
GAAACTAAAG GCTGTCAAAC AATAACTTCT GGCAAAGTGC GTCATGGAGC TCTTACATGC 
TTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACGCGT 
CACAGAAAAT TTAAGGAAAT TCAAGTCCCG TACAACGTCC AGTGGATGGC GATCTTCAGT 
GAGCAGCTCT GTGTGGGATT CCAGTCTGGA TTTCTAAGAT ATCCCCTGAA TGGAGAAGGA 
AGTCCATACA GTATGCTCCA TTCAAATGAC AATACACTCT CATTTATTGC ACATCAACCA 
ATGGATGCCA TCTGTGCGGT TGAAATCTCC AGTAAAGAAT ACCTGCTGTG TTTTAACAGC 
ATTGGAATAT ACACTGACTG CCAGGGCCGA AGATCTAGAC AACAGGAACT GATGTGGCCA 
GCGAATCCTT CCTCTTGTTG TTACAATGCA CCGTATCTCT CAGTGTACAG TGAAAACGCA 
GTTGACATCT TTGATGTGAA CTCCATGGAA TGGATTCAGA CTCTCCCACT CAAAAAGGTT 
CGCCCCTTAA ACAGTGAAGG ATCATTAAAT CTTTTAGGCT TGGAGACGAT TAGATTGATA 
TATTTCAAAA ATAAAATGGC AGAAGGGGAT GAACTGGTAG TTCCTGAAAC ATCGGATAAT 
AGCCGGAAGC AAATGGTTAG AAATATTAAC AATAAGAGGC GTTACTCTTT CAGAGTTCCG 
GAAGAGGAAA GAATGCAACA GAGGAGGGAG ATGTTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACATA TGGGTCCAGG AGATGGAATA 
CAGATCCTAA AAGACCTGCC CATGAACCCT CGGCCTCAGG AAAGTCGGAC AGCATTCAGT 
GGCTCGGTCA GTATTCCGTC TATCACCAAA TCCCGCCCTG AACCCGGTCG CTCCATGAGC 
GCCAGCAGTG GCCTATCTGC AAGGTCATCT GCACAGAATG GCAGTGCGTT AAAGAGAGAA 
TTCTCTGGAG GCAGCTACAG TGCAAAGCGG CAGCCCATGC CCTCCCCATC GGAGGGCTCG 
CTGTCCTCAG GAGGCATGGA CCAGGGAAGT GACGCCCCAG CGCGGGACTA TGACGGAGAG 
GACTCTGATT CTCCGCGGCA TTCCACGGCT TCCAACAGTT CCAACCTGAG CAGTCCTCCC 
AGCCCGGTGT CGCCCCGGAA GACCAAGAGC CTGTCTCTGG AGAGCACAGA CCGCGGGAGC 
TGGGACCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062496.1
CDS	766..6117
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062434.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062434.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGATCCC 
GTTGAGAACA CATATGTACG GAATCCGAAC GTCAAGTTTT ACATCCAGTC AAGGTCCACC 
TCTCCTTCCA CATCTAGTGA AGCTGAGCCA GTTAAGACTG TAGACTCTAC TCCACTTCCA 
GTTCATACAC CAACCTTAAG GAAAAAGGGA TGCCCTGGCT CAACTGGCTT TCCATCGAAG 
CGCAAGATTC ATCAGTTTTT TGTCAAATCT TTTACTACTC CCACCAGGTG TCATCAGTGT 
ACATCTTTGA TGGTGGGTTT GATAAGACAA GGCTGTTCTT GTGAAGTGTG TGGATTTTCA 
TGTCACATAA CTTGTGTAAA CAAAGCTCCA ACTGCTTGTC CAGTTCCTCC TGAACAGACA 
AAAGGTCCTC TGGGTATAGA TCCACAGAAA GGAATAGGAA CAGCATATGA AGGGCATGTC 
AGGATCCCTA AGCCAGCTGG AGTGAAGAAA GGATGGCAAA GAGCACTGGC TGTAGTCTGT 
GACTTCAAGC TCTTTCTGTA CGATATTGCC GAAGGAAAGG CATCTCAGCC CAGTGTTGTA 
GTCAGTCAAG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT CTTGGCTTCT 
GATGTTATTC ATGCAAGTCG AAAAGATATA CCCTGTATAT TTAGAGTCAC AGCTTCCCAG 
CTCTCAGCAT CTAGTAACAA ATGTTCCATC CTGATGCTAG CTGATAGTGA AAATGAGAAG 
AGTAAATGGG TGGGAGTACT GAGTGAATTG CACAAGATTT TGAAGAAAAA CAAATTCAGA 
GACCGCTCAG TCTATGTTCC CAAAGAGGCT TATGACAGCA CTCTGCCCCT CATTAAAACA 
ACCCAGGCAG CTGCAATCAT AGATCATGAA AGAATAGCTT TGGGGAATGA AGAAGGGTTA 
TTTGTTGTGC ATGTCACCAA AGATGAAATT ATTAGAGTTG GTGACAATAA GAAGATTCAT 
CAGATAGAAC TCATTCCAAA TGATCAACTT GTTGCCGTGA TCTCAGGACG AAATCGACAC 
GTCCGACTTT TTCCTATGTC AGCTCTGGAC GGGCGAGAGA CTGATTTTTA TAAGCTGGCA 
GAAACTAAAG GCTGTCAAAC AATAACTTCT GGCAAAGTGC GTCATGGAGC TCTTACATGC 
TTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACGCGT 
CACAGAAAAT TTAAGGAAAT TCAAGTCCCG TACAACGTCC AGTGGATGGC GATCTTCAGT 
GAGCAGCTCT GTGTGGGATT CCAGTCTGGA TTTCTAAGAT ATCCCCTGAA TGGAGAAGGA 
AGTCCATACA GTATGCTCCA TTCAAATGAC AATACACTCT CATTTATTGC ACATCAACCA 
ATGGATGCCA TCTGTGCGGT TGAAATCTCC AGTAAAGAAT ACCTGCTGTG TTTTAACAGC 
ATTGGAATAT ACACTGACTG CCAGGGCCGA AGATCTAGAC AACAGGAACT GATGTGGCCA 
GCGAATCCTT CCTCTTGTTG TTACAATGCA CCGTATCTCT CAGTGTACAG TGAAAACGCA 
GTTGACATCT TTGATGTGAA CTCCATGGAA TGGATTCAGA CTCTCCCACT CAAAAAGGTT 
CGCCCCTTAA ACAGTGAAGG ATCATTAAAT CTTTTAGGCT TGGAGACGAT TAGATTGATA 
TATTTCAAAA ATAAAATGGC AGAAGGGGAT GAACTGGTAG TTCCTGAAAC ATCGGATAAT 
AGCCGGAAGC AAATGGTTAG AAATATTAAC AATAAGAGGC GTTACTCTTT CAGAGTTCCG 
GAAGAGGAAA GAATGCAACA GAGGAGGGAG ATGTTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACATA TGGGTCCAGG AGATGGAATA 
CAGATCCTAA AAGACCTGCC CATGAACCCT CGGCCTCAGG AAAGTCGGAC AGCATTCAGT 
GGCTCGGTCA GTATTCCGTC TATCACCAAA TCCCGCCCTG AACCCGGTCG CTCCATGAGC 
GCCAGCAGTG GCCTATCTGC AAGGTCATCT GCACAGAATG GCAGTGCGTT AAAGAGAGAA 
TTCTCTGGAG GCAGCTACAG TGCAAAGCGG CAGCCCATGC CCTCCCCATC GGAGGGCTCG 
CTGTCCTCAG GAGGCATGGA CCAGGGAAGT GACGCCCCAG CGCGGGACTA TGACGGAGAG 
GACTCTGATT CTCCGCGGCA TTCCACGGCT TCCAACAGTT CCAACCTGAG CAGTCCTCCC 
AGCCCGGTGT CGCCCCGGAA GACCAAGAGC CTGTCTCTGG AGAGCACAGA CCGCGGGAGC 
TGGGACCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062434.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062496.1 Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X9, mRNA.

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CloneID	OBu18511
Clone ID Related Accession (Same CDS sequence)	XM_006062434.2 , XM_006062434.1
Accession Version	XM_006062434.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGATCCC 
GTTGAGAACA CATATGTACG GAATCCGAAC GTCAAGTTTT ACATCCAGTC AAGGTCCACC 
TCTCCTTCCA CATCTAGTGA AGCTGAGCCA GTTAAGACTG TAGACTCTAC TCCACTTCCA 
GTTCATACAC CAACCTTAAG GAAAAAGGGA TGCCCTGGCT CAACTGGCTT TCCATCGAAG 
CGCAAGATTC ATCAGTTTTT TGTCAAATCT TTTACTACTC CCACCAGGTG TCATCAGTGT 
ACATCTTTGA TGGTGGGTTT GATAAGACAA GGCTGTTCTT GTGAAGTGTG TGGATTTTCA 
TGTCACATAA CTTGTGTAAA CAAAGCTCCA ACTGCTTGTC CAGTTCCTCC TGAACAGACA 
AAAGGTCCTC TGGGTATAGA TCCACAGAAA GGAATAGGAA CAGCATATGA AGGGCATGTC 
AGGATCCCTA AGCCAGCTGG AGTGAAGAAA GGATGGCAAA GAGCACTGGC TGTAGTCTGT 
GACTTCAAGC TCTTTCTGTA CGATATTGCC GAAGGAAAGG CATCTCAGCC CAGTGTTGTA 
GTCAGTCAAG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT CTTGGCTTCT 
GATGTTATTC ATGCAAGTCG AAAAGATATA CCCTGTATAT TTAGAGTCAC AGCTTCCCAG 
CTCTCAGCAT CTAGTAACAA ATGTTCCATC CTGATGCTAG CTGATAGTGA AAATGAGAAG 
AGTAAATGGG TGGGAGTACT GAGTGAATTG CACAAGATTT TGAAGAAAAA CAAATTCAGA 
GACCGCTCAG TCTATGTTCC CAAAGAGGCT TATGACAGCA CTCTGCCCCT CATTAAAACA 
ACCCAGGCAG CTGCAATCAT AGATCATGAA AGAATAGCTT TGGGGAATGA AGAAGGGTTA 
TTTGTTGTGC ATGTCACCAA AGATGAAATT ATTAGAGTTG GTGACAATAA GAAGATTCAT 
CAGATAGAAC TCATTCCAAA TGATCAACTT GTTGCCGTGA TCTCAGGACG AAATCGACAC 
GTCCGACTTT TTCCTATGTC AGCTCTGGAC GGGCGAGAGA CTGATTTTTA TAAGCTGGCA 
GAAACTAAAG GCTGTCAAAC AATAACTTCT GGCAAAGTGC GTCATGGAGC TCTTACATGC 
TTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACGCGT 
CACAGAAAAT TTAAGGAAAT TCAAGTCCCG TACAACGTCC AGTGGATGGC GATCTTCAGT 
GAGCAGCTCT GTGTGGGATT CCAGTCTGGA TTTCTAAGAT ATCCCCTGAA TGGAGAAGGA 
AGTCCATACA GTATGCTCCA TTCAAATGAC AATACACTCT CATTTATTGC ACATCAACCA 
ATGGATGCCA TCTGTGCGGT TGAAATCTCC AGTAAAGAAT ACCTGCTGTG TTTTAACAGC 
ATTGGAATAT ACACTGACTG CCAGGGCCGA AGATCTAGAC AACAGGAACT GATGTGGCCA 
GCGAATCCTT CCTCTTGTTG TTACAATGCA CCGTATCTCT CAGTGTACAG TGAAAACGCA 
GTTGACATCT TTGATGTGAA CTCCATGGAA TGGATTCAGA CTCTCCCACT CAAAAAGGTT 
CGCCCCTTAA ACAGTGAAGG ATCATTAAAT CTTTTAGGCT TGGAGACGAT TAGATTGATA 
TATTTCAAAA ATAAAATGGC AGAAGGGGAT GAACTGGTAG TTCCTGAAAC ATCGGATAAT 
AGCCGGAAGC AAATGGTTAG AAATATTAAC AATAAGAGGC GTTACTCTTT CAGAGTTCCG 
GAAGAGGAAA GAATGCAACA GAGGAGGGAG ATGTTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACATA TGGGTCCAGG AGATGGAATA 
CAGATCCTAA AAGACCTGCC CATGAACCCT CGGCCTCAGG AAAGTCGGAC AGCATTCAGT 
GGCTCGGTCA GTATTCCGTC TATCACCAAA TCCCGCCCTG AACCCGGTCG CTCCATGAGC 
GCCAGCAGTG GCCTATCTGC AAGGTCATCT GCACAGAATG GCAGTGCGTT AAAGAGAGAA 
TTCTCTGGAG GCAGCTACAG TGCAAAGCGG CAGCCCATGC CCTCCCCATC GGAGGGCTCG 
CTGTCCTCAG GAGGCATGGA CCAGGGAAGT GACGCCCCAG CGCGGGACTA TGACGGAGAG 
GACTCTGATT CTCCGCGGCA TTCCACGGCT TCCAACAGTT CCAACCTGAG CAGTCCTCCC 
AGCCCGGTGT CGCCCCGGAA GACCAAGAGC CTGTCTCTGG AGAGCACAGA CCGCGGGAGC 
TGGGACCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062496.1
CDS	289..5640
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062434.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062434.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGATCCC 
GTTGAGAACA CATATGTACG GAATCCGAAC GTCAAGTTTT ACATCCAGTC AAGGTCCACC 
TCTCCTTCCA CATCTAGTGA AGCTGAGCCA GTTAAGACTG TAGACTCTAC TCCACTTCCA 
GTTCATACAC CAACCTTAAG GAAAAAGGGA TGCCCTGGCT CAACTGGCTT TCCATCGAAG 
CGCAAGATTC ATCAGTTTTT TGTCAAATCT TTTACTACTC CCACCAGGTG TCATCAGTGT 
ACATCTTTGA TGGTGGGTTT GATAAGACAA GGCTGTTCTT GTGAAGTGTG TGGATTTTCA 
TGTCACATAA CTTGTGTAAA CAAAGCTCCA ACTGCTTGTC CAGTTCCTCC TGAACAGACA 
AAAGGTCCTC TGGGTATAGA TCCACAGAAA GGAATAGGAA CAGCATATGA AGGGCATGTC 
AGGATCCCTA AGCCAGCTGG AGTGAAGAAA GGATGGCAAA GAGCACTGGC TGTAGTCTGT 
GACTTCAAGC TCTTTCTGTA CGATATTGCC GAAGGAAAGG CATCTCAGCC CAGTGTTGTA 
GTCAGTCAAG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT CTTGGCTTCT 
GATGTTATTC ATGCAAGTCG AAAAGATATA CCCTGTATAT TTAGAGTCAC AGCTTCCCAG 
CTCTCAGCAT CTAGTAACAA ATGTTCCATC CTGATGCTAG CTGATAGTGA AAATGAGAAG 
AGTAAATGGG TGGGAGTACT GAGTGAATTG CACAAGATTT TGAAGAAAAA CAAATTCAGA 
GACCGCTCAG TCTATGTTCC CAAAGAGGCT TATGACAGCA CTCTGCCCCT CATTAAAACA 
ACCCAGGCAG CTGCAATCAT AGATCATGAA AGAATAGCTT TGGGGAATGA AGAAGGGTTA 
TTTGTTGTGC ATGTCACCAA AGATGAAATT ATTAGAGTTG GTGACAATAA GAAGATTCAT 
CAGATAGAAC TCATTCCAAA TGATCAACTT GTTGCCGTGA TCTCAGGACG AAATCGACAC 
GTCCGACTTT TTCCTATGTC AGCTCTGGAC GGGCGAGAGA CTGATTTTTA TAAGCTGGCA 
GAAACTAAAG GCTGTCAAAC AATAACTTCT GGCAAAGTGC GTCATGGAGC TCTTACATGC 
TTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACGCGT 
CACAGAAAAT TTAAGGAAAT TCAAGTCCCG TACAACGTCC AGTGGATGGC GATCTTCAGT 
GAGCAGCTCT GTGTGGGATT CCAGTCTGGA TTTCTAAGAT ATCCCCTGAA TGGAGAAGGA 
AGTCCATACA GTATGCTCCA TTCAAATGAC AATACACTCT CATTTATTGC ACATCAACCA 
ATGGATGCCA TCTGTGCGGT TGAAATCTCC AGTAAAGAAT ACCTGCTGTG TTTTAACAGC 
ATTGGAATAT ACACTGACTG CCAGGGCCGA AGATCTAGAC AACAGGAACT GATGTGGCCA 
GCGAATCCTT CCTCTTGTTG TTACAATGCA CCGTATCTCT CAGTGTACAG TGAAAACGCA 
GTTGACATCT TTGATGTGAA CTCCATGGAA TGGATTCAGA CTCTCCCACT CAAAAAGGTT 
CGCCCCTTAA ACAGTGAAGG ATCATTAAAT CTTTTAGGCT TGGAGACGAT TAGATTGATA 
TATTTCAAAA ATAAAATGGC AGAAGGGGAT GAACTGGTAG TTCCTGAAAC ATCGGATAAT 
AGCCGGAAGC AAATGGTTAG AAATATTAAC AATAAGAGGC GTTACTCTTT CAGAGTTCCG 
GAAGAGGAAA GAATGCAACA GAGGAGGGAG ATGTTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACATA TGGGTCCAGG AGATGGAATA 
CAGATCCTAA AAGACCTGCC CATGAACCCT CGGCCTCAGG AAAGTCGGAC AGCATTCAGT 
GGCTCGGTCA GTATTCCGTC TATCACCAAA TCCCGCCCTG AACCCGGTCG CTCCATGAGC 
GCCAGCAGTG GCCTATCTGC AAGGTCATCT GCACAGAATG GCAGTGCGTT AAAGAGAGAA 
TTCTCTGGAG GCAGCTACAG TGCAAAGCGG CAGCCCATGC CCTCCCCATC GGAGGGCTCG 
CTGTCCTCAG GAGGCATGGA CCAGGGAAGT GACGCCCCAG CGCGGGACTA TGACGGAGAG 
GACTCTGATT CTCCGCGGCA TTCCACGGCT TCCAACAGTT CCAACCTGAG CAGTCCTCCC 
AGCCCGGTGT CGCCCCGGAA GACCAAGAGC CTGTCTCTGG AGAGCACAGA CCGCGGGAGC 
TGGGACCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062435.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062497.1 Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X10, mRNA.

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CloneID	OBu18512
Clone ID Related Accession (Same CDS sequence)	XM_006062435.1 , XM_006062435.2
Accession Version	XM_006062435.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGCTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAGGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAATGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG CAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCGTA CAACGTCCAG TGGATGGCGA TCTTCAGTGA GCAGCTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAACGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CGGATAATAG CCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCGGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGCATT CAGTGGCTCG 
GTCAGTATTC CGTCTATCAC CAAATCCCGC CCTGAACCCG GTCGCTCCAT GAGCGCCAGC 
AGTGGCCTAT CTGCAAGGTC ATCTGCACAG AATGGCAGTG CGTTAAAGAG AGAATTCTCT 
GGAGGCAGCT ACAGTGCAAA GCGGCAGCCC ATGCCCTCCC CATCGGAGGG CTCGCTGTCC 
TCAGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCGCGGG ACTATGACGG AGAGGACTCT 
GATTCTCCGC GGCATTCCAC GGCTTCCAAC AGTTCCAACC TGAGCAGTCC TCCCAGCCCG 
GTGTCGCCCC GGAAGACCAA GAGCCTGTCT CTGGAGAGCA CAGACCGCGG GAGCTGGGAC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062497.1
CDS	289..5634
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062435.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062435.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGCTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAGGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAATGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG CAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCGTA CAACGTCCAG TGGATGGCGA TCTTCAGTGA GCAGCTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAACGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CGGATAATAG CCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCGGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGCATT CAGTGGCTCG 
GTCAGTATTC CGTCTATCAC CAAATCCCGC CCTGAACCCG GTCGCTCCAT GAGCGCCAGC 
AGTGGCCTAT CTGCAAGGTC ATCTGCACAG AATGGCAGTG CGTTAAAGAG AGAATTCTCT 
GGAGGCAGCT ACAGTGCAAA GCGGCAGCCC ATGCCCTCCC CATCGGAGGG CTCGCTGTCC 
TCAGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCGCGGG ACTATGACGG AGAGGACTCT 
GATTCTCCGC GGCATTCCAC GGCTTCCAAC AGTTCCAACC TGAGCAGTCC TCCCAGCCCG 
GTGTCGCCCC GGAAGACCAA GAGCCTGTCT CTGGAGAGCA CAGACCGCGG GAGCTGGGAC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062435.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062497.1 Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X9, mRNA.

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CloneID	OBu18512
Clone ID Related Accession (Same CDS sequence)	XM_006062435.1 , XM_006062435.2
Accession Version	XM_006062435.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006062435.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGCTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAGGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAATGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG CAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCGTA CAACGTCCAG TGGATGGCGA TCTTCAGTGA GCAGCTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAACGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CGGATAATAG CCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCGGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGCATT CAGTGGCTCG 
GTCAGTATTC CGTCTATCAC CAAATCCCGC CCTGAACCCG GTCGCTCCAT GAGCGCCAGC 
AGTGGCCTAT CTGCAAGGTC ATCTGCACAG AATGGCAGTG CGTTAAAGAG AGAATTCTCT 
GGAGGCAGCT ACAGTGCAAA GCGGCAGCCC ATGCCCTCCC CATCGGAGGG CTCGCTGTCC 
TCAGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCGCGGG ACTATGACGG AGAGGACTCT 
GATTCTCCGC GGCATTCCAC GGCTTCCAAC AGTTCCAACC TGAGCAGTCC TCCCAGCCCG 
GTGTCGCCCC GGAAGACCAA GAGCCTGTCT CTGGAGAGCA CAGACCGCGG GAGCTGGGAC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062497.1
CDS	766..6111
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062435.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062435.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGCTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAGGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAATGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG CAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCGTA CAACGTCCAG TGGATGGCGA TCTTCAGTGA GCAGCTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAACGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CGGATAATAG CCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCGGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGCATT CAGTGGCTCG 
GTCAGTATTC CGTCTATCAC CAAATCCCGC CCTGAACCCG GTCGCTCCAT GAGCGCCAGC 
AGTGGCCTAT CTGCAAGGTC ATCTGCACAG AATGGCAGTG CGTTAAAGAG AGAATTCTCT 
GGAGGCAGCT ACAGTGCAAA GCGGCAGCCC ATGCCCTCCC CATCGGAGGG CTCGCTGTCC 
TCAGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCGCGGG ACTATGACGG AGAGGACTCT 
GATTCTCCGC GGCATTCCAC GGCTTCCAAC AGTTCCAACC TGAGCAGTCC TCCCAGCCCG 
GTGTCGCCCC GGAAGACCAA GAGCCTGTCT CTGGAGAGCA CAGACCGCGG GAGCTGGGAC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062436.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062498.1 Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X11, mRNA.

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CloneID	OBu18513
Clone ID Related Accession (Same CDS sequence)	XM_006062436.2 , XM_006062436.1
Accession Version	XM_006062436.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006062436.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGCTG GAAGTTCGTA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ACAGGAAATT GCGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAGAAT 
GAACTAGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGCGTATG CAGCATAGAA 
CATCAGCAAG AAATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTGT CCAAGAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTACATGATT CAGAAGGCCA ACAACTTGCT CTCAACAAAG AAATTATGAT TTTGAAAGAC 
AAATTGGAAA AAAACAGGAG AGAGAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAGCAACAGT ATGAACGAGA AAAAGTGTTA CTAACTGAAG AAAATAAAAA ACTGACAAGT 
GAGCTTGATA AGCTTACCAC TCTGTGTGAG AACTTAAGTG CACGCAACCA GCAGTTAGAA 
GAAGAGGTCA AAGATCTAGC AGATAAGAAG GAATCGGTTG CTCATTGGGA GGCTCAGATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT TCAAGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTAAGAAACT CCAGCTTGGG GACCCGAGCA 
ACAGATATGC CCTGGAAAAT GCGACGTTTT GCAAAACTGG ATATGTCAGC CAGACTGGAG 
TTACAGTCAG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGCTGAAT 
AAAGTTAAAG CATCTAACAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TATCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AGATTCCTTT TCTTCTTCCT CATCTTCACT GATTGATTTT 
TTGGATGACC GCTCTCCATC CTGTACTCCA GCTAGCAAAG GCAGACGTAC TGATCCCGTT 
GAGAACACAT ATGTACGGAA TCCGAACGTC AAGTTTTACA TCCAGTCAAG GTCCACCTCT 
CCTTCCACAT CTAGTGAAGC TGAGCCAGTT AAGACTGTAG ACTCTACTCC ACTTCCAGTT 
CATACACCAA CCTTAAGGAA AAAGGGATGC CCTGGCTCAA CTGGCTTTCC ATCGAAGCGC 
AAGATTCATC AGTTTTTTGT CAAATCTTTT ACTACTCCCA CCAGGTGTCA TCAGTGTACA 
TCTTTGATGG TGGGTTTGAT AAGACAAGGC TGTTCTTGTG AAGTGTGTGG ATTTTCATGT 
CACATAACTT GTGTAAACAA AGCTCCAACT GCTTGTCCAG TTCCTCCTGA ACAGACAAAA 
GGTCCTCTGG GTATAGATCC ACAGAAAGGA ATAGGAACAG CATATGAAGG GCATGTCAGG 
ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA TGGCAAAGAG CACTGGCTGT AGTCTGTGAC 
TTCAAGCTCT TTCTGTACGA TATTGCCGAA GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC 
AGTCAAGTGA TTGACATGAG GGATGAAGAA TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT 
GTTATTCATG CAAGTCGAAA AGATATACCC TGTATATTTA GAGTCACAGC TTCCCAGCTC 
TCAGCATCTA GTAACAAATG TTCCATCCTG ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT 
AAATGGGTGG GAGTACTGAG TGAATTGCAC AAGATTTTGA AGAAAAACAA ATTCAGAGAC 
CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT GACAGCACTC TGCCCCTCAT TAAAACAACC 
CAGGCAGCTG CAATCATAGA TCATGAAAGA ATAGCTTTGG GGAATGAAGA AGGGTTATTT 
GTTGTGCATG TCACCAAAGA TGAAATTATT AGAGTTGGTG ACAATAAGAA GATTCATCAG 
ATAGAACTCA TTCCAAATGA TCAACTTGTT GCCGTGATCT CAGGACGAAA TCGACACGTC 
CGACTTTTTC CTATGTCAGC TCTGGACGGG CGAGAGACTG ATTTTTATAA GCTGGCAGAA 
ACTAAAGGCT GTCAAACAAT AACTTCTGGC AAAGTGCGTC ATGGAGCTCT TACATGCTTG 
TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT TATGAACTAT TTCAGAGCAA GACGCGTCAC 
AGAAAATTTA AGGAAATTCA AGTCCCGTAC AACGTCCAGT GGATGGCGAT CTTCAGTGAG 
CAGCTCTGTG TGGGATTCCA GTCTGGATTT CTAAGATATC CCCTGAATGG AGAAGGAAGT 
CCATACAGTA TGCTCCATTC AAATGACAAT ACACTCTCAT TTATTGCACA TCAACCAATG 
GATGCCATCT GTGCGGTTGA AATCTCCAGT AAAGAATACC TGCTGTGTTT TAACAGCATT 
GGAATATACA CTGACTGCCA GGGCCGAAGA TCTAGACAAC AGGAACTGAT GTGGCCAGCG 
AATCCTTCCT CTTGTTGTTA CAATGCACCG TATCTCTCAG TGTACAGTGA AAACGCAGTT 
GACATCTTTG ATGTGAACTC CATGGAATGG ATTCAGACTC TCCCACTCAA AAAGGTTCGC 
CCCTTAAACA GTGAAGGATC ATTAAATCTT TTAGGCTTGG AGACGATTAG ATTGATATAT 
TTCAAAAATA AAATGGCAGA AGGGGATGAA CTGGTAGTTC CTGAAACATC GGATAATAGC 
CGGAAGCAAA TGGTTAGAAA TATTAACAAT AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA 
GAGGAAAGAA TGCAACAGAG GAGGGAGATG TTACGAGATC CAGAAATGAG AAATAAATTA 
ATTTCTAACC CAACTAATTT TAACCACATA GCACATATGG GTCCAGGAGA TGGAATACAG 
ATCCTAAAAG ACCTGCCCAT GCCGGGTTTC CCTTATCCAT CCCCTCATCA TCACTCCGGT 
CTGATCTCCT CTCCGATCAA CTTTGAGCAC ATCTATCACA TGACTGTTAA TTCTGCTGAA 
AAATTTCTCT CTCCTGATTC CATAAACCCT GAATATTCCC CGTCTCTGCG CTCTGTCCCT 
GGTACACCAA GCTTTATGAC TCTGAGGAAC CCTCGGCCTC AGGAAAGTCG GACAGCATTC 
AGTGGCTCGG TCAGTATTCC GTCTATCACC AAATCCCGCC CTGAACCCGG TCGCTCCATG 
AGCGCCAGCA GTGGCCTATC TGCAAGGTCA TCTGCACAGA ATGGCAGTGC GTTAAAGAGA 
GAATTCTCTG GAGGCAGCTA CAGTGCAAAG CGGCAGCCCA TGCCCTCCCC ATCGGAGGGC 
TCGCTGTCCT CAGGAGGCAT GGACCAGGGA AGTGACGCCC CAGCGCGGGA CTATGACGGA 
GAGGACTCTG ATTCTCCGCG GCATTCCACG GCTTCCAACA GTTCCAACCT GAGCAGTCCT 
CCCAGCCCGG TGTCGCCCCG GAAGACCAAG AGCCTGTCTC TGGAGAGCAC AGACCGCGGG 
AGCTGGGACC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062498.1
CDS	766..6060
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKLEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062436.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062436.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGCTG GAAGTTCGTA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ACAGGAAATT GCGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAGAAT 
GAACTAGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGCGTATG CAGCATAGAA 
CATCAGCAAG AAATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTGT CCAAGAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTACATGATT CAGAAGGCCA ACAACTTGCT CTCAACAAAG AAATTATGAT TTTGAAAGAC 
AAATTGGAAA AAAACAGGAG AGAGAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAGCAACAGT ATGAACGAGA AAAAGTGTTA CTAACTGAAG AAAATAAAAA ACTGACAAGT 
GAGCTTGATA AGCTTACCAC TCTGTGTGAG AACTTAAGTG CACGCAACCA GCAGTTAGAA 
GAAGAGGTCA AAGATCTAGC AGATAAGAAG GAATCGGTTG CTCATTGGGA GGCTCAGATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT TCAAGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTAAGAAACT CCAGCTTGGG GACCCGAGCA 
ACAGATATGC CCTGGAAAAT GCGACGTTTT GCAAAACTGG ATATGTCAGC CAGACTGGAG 
TTACAGTCAG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGCTGAAT 
AAAGTTAAAG CATCTAACAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TATCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AGATTCCTTT TCTTCTTCCT CATCTTCACT GATTGATTTT 
TTGGATGACC GCTCTCCATC CTGTACTCCA GCTAGCAAAG GCAGACGTAC TGATCCCGTT 
GAGAACACAT ATGTACGGAA TCCGAACGTC AAGTTTTACA TCCAGTCAAG GTCCACCTCT 
CCTTCCACAT CTAGTGAAGC TGAGCCAGTT AAGACTGTAG ACTCTACTCC ACTTCCAGTT 
CATACACCAA CCTTAAGGAA AAAGGGATGC CCTGGCTCAA CTGGCTTTCC ATCGAAGCGC 
AAGATTCATC AGTTTTTTGT CAAATCTTTT ACTACTCCCA CCAGGTGTCA TCAGTGTACA 
TCTTTGATGG TGGGTTTGAT AAGACAAGGC TGTTCTTGTG AAGTGTGTGG ATTTTCATGT 
CACATAACTT GTGTAAACAA AGCTCCAACT GCTTGTCCAG TTCCTCCTGA ACAGACAAAA 
GGTCCTCTGG GTATAGATCC ACAGAAAGGA ATAGGAACAG CATATGAAGG GCATGTCAGG 
ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA TGGCAAAGAG CACTGGCTGT AGTCTGTGAC 
TTCAAGCTCT TTCTGTACGA TATTGCCGAA GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC 
AGTCAAGTGA TTGACATGAG GGATGAAGAA TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT 
GTTATTCATG CAAGTCGAAA AGATATACCC TGTATATTTA GAGTCACAGC TTCCCAGCTC 
TCAGCATCTA GTAACAAATG TTCCATCCTG ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT 
AAATGGGTGG GAGTACTGAG TGAATTGCAC AAGATTTTGA AGAAAAACAA ATTCAGAGAC 
CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT GACAGCACTC TGCCCCTCAT TAAAACAACC 
CAGGCAGCTG CAATCATAGA TCATGAAAGA ATAGCTTTGG GGAATGAAGA AGGGTTATTT 
GTTGTGCATG TCACCAAAGA TGAAATTATT AGAGTTGGTG ACAATAAGAA GATTCATCAG 
ATAGAACTCA TTCCAAATGA TCAACTTGTT GCCGTGATCT CAGGACGAAA TCGACACGTC 
CGACTTTTTC CTATGTCAGC TCTGGACGGG CGAGAGACTG ATTTTTATAA GCTGGCAGAA 
ACTAAAGGCT GTCAAACAAT AACTTCTGGC AAAGTGCGTC ATGGAGCTCT TACATGCTTG 
TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT TATGAACTAT TTCAGAGCAA GACGCGTCAC 
AGAAAATTTA AGGAAATTCA AGTCCCGTAC AACGTCCAGT GGATGGCGAT CTTCAGTGAG 
CAGCTCTGTG TGGGATTCCA GTCTGGATTT CTAAGATATC CCCTGAATGG AGAAGGAAGT 
CCATACAGTA TGCTCCATTC AAATGACAAT ACACTCTCAT TTATTGCACA TCAACCAATG 
GATGCCATCT GTGCGGTTGA AATCTCCAGT AAAGAATACC TGCTGTGTTT TAACAGCATT 
GGAATATACA CTGACTGCCA GGGCCGAAGA TCTAGACAAC AGGAACTGAT GTGGCCAGCG 
AATCCTTCCT CTTGTTGTTA CAATGCACCG TATCTCTCAG TGTACAGTGA AAACGCAGTT 
GACATCTTTG ATGTGAACTC CATGGAATGG ATTCAGACTC TCCCACTCAA AAAGGTTCGC 
CCCTTAAACA GTGAAGGATC ATTAAATCTT TTAGGCTTGG AGACGATTAG ATTGATATAT 
TTCAAAAATA AAATGGCAGA AGGGGATGAA CTGGTAGTTC CTGAAACATC GGATAATAGC 
CGGAAGCAAA TGGTTAGAAA TATTAACAAT AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA 
GAGGAAAGAA TGCAACAGAG GAGGGAGATG TTACGAGATC CAGAAATGAG AAATAAATTA 
ATTTCTAACC CAACTAATTT TAACCACATA GCACATATGG GTCCAGGAGA TGGAATACAG 
ATCCTAAAAG ACCTGCCCAT GCCGGGTTTC CCTTATCCAT CCCCTCATCA TCACTCCGGT 
CTGATCTCCT CTCCGATCAA CTTTGAGCAC ATCTATCACA TGACTGTTAA TTCTGCTGAA 
AAATTTCTCT CTCCTGATTC CATAAACCCT GAATATTCCC CGTCTCTGCG CTCTGTCCCT 
GGTACACCAA GCTTTATGAC TCTGAGGAAC CCTCGGCCTC AGGAAAGTCG GACAGCATTC 
AGTGGCTCGG TCAGTATTCC GTCTATCACC AAATCCCGCC CTGAACCCGG TCGCTCCATG 
AGCGCCAGCA GTGGCCTATC TGCAAGGTCA TCTGCACAGA ATGGCAGTGC GTTAAAGAGA 
GAATTCTCTG GAGGCAGCTA CAGTGCAAAG CGGCAGCCCA TGCCCTCCCC ATCGGAGGGC 
TCGCTGTCCT CAGGAGGCAT GGACCAGGGA AGTGACGCCC CAGCGCGGGA CTATGACGGA 
GAGGACTCTG ATTCTCCGCG GCATTCCACG GCTTCCAACA GTTCCAACCT GAGCAGTCCT 
CCCAGCCCGG TGTCGCCCCG GAAGACCAAG AGCCTGTCTC TGGAGAGCAC AGACCGCGGG 
AGCTGGGACC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062436.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062498.1 Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X11, mRNA.

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CloneID	OBu18513
Clone ID Related Accession (Same CDS sequence)	XM_006062436.2 , XM_006062436.1
Accession Version	XM_006062436.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGCTG GAAGTTCGTA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ACAGGAAATT GCGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAGAAT 
GAACTAGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGCGTATG CAGCATAGAA 
CATCAGCAAG AAATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTGT CCAAGAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTACATGATT CAGAAGGCCA ACAACTTGCT CTCAACAAAG AAATTATGAT TTTGAAAGAC 
AAATTGGAAA AAAACAGGAG AGAGAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAGCAACAGT ATGAACGAGA AAAAGTGTTA CTAACTGAAG AAAATAAAAA ACTGACAAGT 
GAGCTTGATA AGCTTACCAC TCTGTGTGAG AACTTAAGTG CACGCAACCA GCAGTTAGAA 
GAAGAGGTCA AAGATCTAGC AGATAAGAAG GAATCGGTTG CTCATTGGGA GGCTCAGATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT TCAAGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTAAGAAACT CCAGCTTGGG GACCCGAGCA 
ACAGATATGC CCTGGAAAAT GCGACGTTTT GCAAAACTGG ATATGTCAGC CAGACTGGAG 
TTACAGTCAG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGCTGAAT 
AAAGTTAAAG CATCTAACAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TATCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AGATTCCTTT TCTTCTTCCT CATCTTCACT GATTGATTTT 
TTGGATGACC GCTCTCCATC CTGTACTCCA GCTAGCAAAG GCAGACGTAC TGATCCCGTT 
GAGAACACAT ATGTACGGAA TCCGAACGTC AAGTTTTACA TCCAGTCAAG GTCCACCTCT 
CCTTCCACAT CTAGTGAAGC TGAGCCAGTT AAGACTGTAG ACTCTACTCC ACTTCCAGTT 
CATACACCAA CCTTAAGGAA AAAGGGATGC CCTGGCTCAA CTGGCTTTCC ATCGAAGCGC 
AAGATTCATC AGTTTTTTGT CAAATCTTTT ACTACTCCCA CCAGGTGTCA TCAGTGTACA 
TCTTTGATGG TGGGTTTGAT AAGACAAGGC TGTTCTTGTG AAGTGTGTGG ATTTTCATGT 
CACATAACTT GTGTAAACAA AGCTCCAACT GCTTGTCCAG TTCCTCCTGA ACAGACAAAA 
GGTCCTCTGG GTATAGATCC ACAGAAAGGA ATAGGAACAG CATATGAAGG GCATGTCAGG 
ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA TGGCAAAGAG CACTGGCTGT AGTCTGTGAC 
TTCAAGCTCT TTCTGTACGA TATTGCCGAA GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC 
AGTCAAGTGA TTGACATGAG GGATGAAGAA TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT 
GTTATTCATG CAAGTCGAAA AGATATACCC TGTATATTTA GAGTCACAGC TTCCCAGCTC 
TCAGCATCTA GTAACAAATG TTCCATCCTG ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT 
AAATGGGTGG GAGTACTGAG TGAATTGCAC AAGATTTTGA AGAAAAACAA ATTCAGAGAC 
CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT GACAGCACTC TGCCCCTCAT TAAAACAACC 
CAGGCAGCTG CAATCATAGA TCATGAAAGA ATAGCTTTGG GGAATGAAGA AGGGTTATTT 
GTTGTGCATG TCACCAAAGA TGAAATTATT AGAGTTGGTG ACAATAAGAA GATTCATCAG 
ATAGAACTCA TTCCAAATGA TCAACTTGTT GCCGTGATCT CAGGACGAAA TCGACACGTC 
CGACTTTTTC CTATGTCAGC TCTGGACGGG CGAGAGACTG ATTTTTATAA GCTGGCAGAA 
ACTAAAGGCT GTCAAACAAT AACTTCTGGC AAAGTGCGTC ATGGAGCTCT TACATGCTTG 
TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT TATGAACTAT TTCAGAGCAA GACGCGTCAC 
AGAAAATTTA AGGAAATTCA AGTCCCGTAC AACGTCCAGT GGATGGCGAT CTTCAGTGAG 
CAGCTCTGTG TGGGATTCCA GTCTGGATTT CTAAGATATC CCCTGAATGG AGAAGGAAGT 
CCATACAGTA TGCTCCATTC AAATGACAAT ACACTCTCAT TTATTGCACA TCAACCAATG 
GATGCCATCT GTGCGGTTGA AATCTCCAGT AAAGAATACC TGCTGTGTTT TAACAGCATT 
GGAATATACA CTGACTGCCA GGGCCGAAGA TCTAGACAAC AGGAACTGAT GTGGCCAGCG 
AATCCTTCCT CTTGTTGTTA CAATGCACCG TATCTCTCAG TGTACAGTGA AAACGCAGTT 
GACATCTTTG ATGTGAACTC CATGGAATGG ATTCAGACTC TCCCACTCAA AAAGGTTCGC 
CCCTTAAACA GTGAAGGATC ATTAAATCTT TTAGGCTTGG AGACGATTAG ATTGATATAT 
TTCAAAAATA AAATGGCAGA AGGGGATGAA CTGGTAGTTC CTGAAACATC GGATAATAGC 
CGGAAGCAAA TGGTTAGAAA TATTAACAAT AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA 
GAGGAAAGAA TGCAACAGAG GAGGGAGATG TTACGAGATC CAGAAATGAG AAATAAATTA 
ATTTCTAACC CAACTAATTT TAACCACATA GCACATATGG GTCCAGGAGA TGGAATACAG 
ATCCTAAAAG ACCTGCCCAT GCCGGGTTTC CCTTATCCAT CCCCTCATCA TCACTCCGGT 
CTGATCTCCT CTCCGATCAA CTTTGAGCAC ATCTATCACA TGACTGTTAA TTCTGCTGAA 
AAATTTCTCT CTCCTGATTC CATAAACCCT GAATATTCCC CGTCTCTGCG CTCTGTCCCT 
GGTACACCAA GCTTTATGAC TCTGAGGAAC CCTCGGCCTC AGGAAAGTCG GACAGCATTC 
AGTGGCTCGG TCAGTATTCC GTCTATCACC AAATCCCGCC CTGAACCCGG TCGCTCCATG 
AGCGCCAGCA GTGGCCTATC TGCAAGGTCA TCTGCACAGA ATGGCAGTGC GTTAAAGAGA 
GAATTCTCTG GAGGCAGCTA CAGTGCAAAG CGGCAGCCCA TGCCCTCCCC ATCGGAGGGC 
TCGCTGTCCT CAGGAGGCAT GGACCAGGGA AGTGACGCCC CAGCGCGGGA CTATGACGGA 
GAGGACTCTG ATTCTCCGCG GCATTCCACG GCTTCCAACA GTTCCAACCT GAGCAGTCCT 
CCCAGCCCGG TGTCGCCCCG GAAGACCAAG AGCCTGTCTC TGGAGAGCAC AGACCGCGGG 
AGCTGGGACC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062498.1
CDS	291..5585
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKLEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062436.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062436.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGCTG GAAGTTCGTA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ACAGGAAATT GCGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAGAAT 
GAACTAGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGCGTATG CAGCATAGAA 
CATCAGCAAG AAATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTGT CCAAGAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTACATGATT CAGAAGGCCA ACAACTTGCT CTCAACAAAG AAATTATGAT TTTGAAAGAC 
AAATTGGAAA AAAACAGGAG AGAGAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAGCAACAGT ATGAACGAGA AAAAGTGTTA CTAACTGAAG AAAATAAAAA ACTGACAAGT 
GAGCTTGATA AGCTTACCAC TCTGTGTGAG AACTTAAGTG CACGCAACCA GCAGTTAGAA 
GAAGAGGTCA AAGATCTAGC AGATAAGAAG GAATCGGTTG CTCATTGGGA GGCTCAGATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT TCAAGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTAAGAAACT CCAGCTTGGG GACCCGAGCA 
ACAGATATGC CCTGGAAAAT GCGACGTTTT GCAAAACTGG ATATGTCAGC CAGACTGGAG 
TTACAGTCAG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGCTGAAT 
AAAGTTAAAG CATCTAACAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TATCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AGATTCCTTT TCTTCTTCCT CATCTTCACT GATTGATTTT 
TTGGATGACC GCTCTCCATC CTGTACTCCA GCTAGCAAAG GCAGACGTAC TGATCCCGTT 
GAGAACACAT ATGTACGGAA TCCGAACGTC AAGTTTTACA TCCAGTCAAG GTCCACCTCT 
CCTTCCACAT CTAGTGAAGC TGAGCCAGTT AAGACTGTAG ACTCTACTCC ACTTCCAGTT 
CATACACCAA CCTTAAGGAA AAAGGGATGC CCTGGCTCAA CTGGCTTTCC ATCGAAGCGC 
AAGATTCATC AGTTTTTTGT CAAATCTTTT ACTACTCCCA CCAGGTGTCA TCAGTGTACA 
TCTTTGATGG TGGGTTTGAT AAGACAAGGC TGTTCTTGTG AAGTGTGTGG ATTTTCATGT 
CACATAACTT GTGTAAACAA AGCTCCAACT GCTTGTCCAG TTCCTCCTGA ACAGACAAAA 
GGTCCTCTGG GTATAGATCC ACAGAAAGGA ATAGGAACAG CATATGAAGG GCATGTCAGG 
ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA TGGCAAAGAG CACTGGCTGT AGTCTGTGAC 
TTCAAGCTCT TTCTGTACGA TATTGCCGAA GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC 
AGTCAAGTGA TTGACATGAG GGATGAAGAA TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT 
GTTATTCATG CAAGTCGAAA AGATATACCC TGTATATTTA GAGTCACAGC TTCCCAGCTC 
TCAGCATCTA GTAACAAATG TTCCATCCTG ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT 
AAATGGGTGG GAGTACTGAG TGAATTGCAC AAGATTTTGA AGAAAAACAA ATTCAGAGAC 
CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT GACAGCACTC TGCCCCTCAT TAAAACAACC 
CAGGCAGCTG CAATCATAGA TCATGAAAGA ATAGCTTTGG GGAATGAAGA AGGGTTATTT 
GTTGTGCATG TCACCAAAGA TGAAATTATT AGAGTTGGTG ACAATAAGAA GATTCATCAG 
ATAGAACTCA TTCCAAATGA TCAACTTGTT GCCGTGATCT CAGGACGAAA TCGACACGTC 
CGACTTTTTC CTATGTCAGC TCTGGACGGG CGAGAGACTG ATTTTTATAA GCTGGCAGAA 
ACTAAAGGCT GTCAAACAAT AACTTCTGGC AAAGTGCGTC ATGGAGCTCT TACATGCTTG 
TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT TATGAACTAT TTCAGAGCAA GACGCGTCAC 
AGAAAATTTA AGGAAATTCA AGTCCCGTAC AACGTCCAGT GGATGGCGAT CTTCAGTGAG 
CAGCTCTGTG TGGGATTCCA GTCTGGATTT CTAAGATATC CCCTGAATGG AGAAGGAAGT 
CCATACAGTA TGCTCCATTC AAATGACAAT ACACTCTCAT TTATTGCACA TCAACCAATG 
GATGCCATCT GTGCGGTTGA AATCTCCAGT AAAGAATACC TGCTGTGTTT TAACAGCATT 
GGAATATACA CTGACTGCCA GGGCCGAAGA TCTAGACAAC AGGAACTGAT GTGGCCAGCG 
AATCCTTCCT CTTGTTGTTA CAATGCACCG TATCTCTCAG TGTACAGTGA AAACGCAGTT 
GACATCTTTG ATGTGAACTC CATGGAATGG ATTCAGACTC TCCCACTCAA AAAGGTTCGC 
CCCTTAAACA GTGAAGGATC ATTAAATCTT TTAGGCTTGG AGACGATTAG ATTGATATAT 
TTCAAAAATA AAATGGCAGA AGGGGATGAA CTGGTAGTTC CTGAAACATC GGATAATAGC 
CGGAAGCAAA TGGTTAGAAA TATTAACAAT AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA 
GAGGAAAGAA TGCAACAGAG GAGGGAGATG TTACGAGATC CAGAAATGAG AAATAAATTA 
ATTTCTAACC CAACTAATTT TAACCACATA GCACATATGG GTCCAGGAGA TGGAATACAG 
ATCCTAAAAG ACCTGCCCAT GCCGGGTTTC CCTTATCCAT CCCCTCATCA TCACTCCGGT 
CTGATCTCCT CTCCGATCAA CTTTGAGCAC ATCTATCACA TGACTGTTAA TTCTGCTGAA 
AAATTTCTCT CTCCTGATTC CATAAACCCT GAATATTCCC CGTCTCTGCG CTCTGTCCCT 
GGTACACCAA GCTTTATGAC TCTGAGGAAC CCTCGGCCTC AGGAAAGTCG GACAGCATTC 
AGTGGCTCGG TCAGTATTCC GTCTATCACC AAATCCCGCC CTGAACCCGG TCGCTCCATG 
AGCGCCAGCA GTGGCCTATC TGCAAGGTCA TCTGCACAGA ATGGCAGTGC GTTAAAGAGA 
GAATTCTCTG GAGGCAGCTA CAGTGCAAAG CGGCAGCCCA TGCCCTCCCC ATCGGAGGGC 
TCGCTGTCCT CAGGAGGCAT GGACCAGGGA AGTGACGCCC CAGCGCGGGA CTATGACGGA 
GAGGACTCTG ATTCTCCGCG GCATTCCACG GCTTCCAACA GTTCCAACCT GAGCAGTCCT 
CCCAGCCCGG TGTCGCCCCG GAAGACCAAG AGCCTGTCTC TGGAGAGCAC AGACCGCGGG 
AGCTGGGACC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062437.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062499.1 Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X12, mRNA.

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CloneID	OBu18514
Clone ID Related Accession (Same CDS sequence)	XM_006062437.1
Accession Version	XM_006062437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGCTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAGGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAATGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG CAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCGTA CAACGTCCAG TGGATGGCGA TCTTCAGTGA GCAGCTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAACGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CGGATAATAG CCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCGGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGCATT CAGTGGCTCG 
GTCAGTATTC CGTCTATCAC CAAATCCCGC CCTGAACCCG GTCGCTCCAT GAGCGCCAGC 
AGTGGCCTAT CTGCAAGGTC ATCTGCACAG AATGGCAGTG CGTTAAAGAG AGAATTCTCT 
GGAGGCAGCT ACAGTGCAAA GCGGCAGCCC ATGCCCTCCC CATCGGAGGG CTCGCTGTCC 
TCAGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCGCGGG ACTATGACGG AGAGGACTCT 
GATTCTCCGC GGCATTCCAC GGCTTCCAAC AGTTCCAACC TGAGCAGTCC TCCCAGCCCG 
GTGTCGCCCC GGAAGACCAA GAGCCTGTCT CTGGAGAGCA CAGACCGCGG GAGCTGGGAC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062499.1
CDS	290..5575
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKASKGRRTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062437.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062437.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGCTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAGGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAATGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG CAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCGTA CAACGTCCAG TGGATGGCGA TCTTCAGTGA GCAGCTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAACGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CGGATAATAG CCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCGGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGCATT CAGTGGCTCG 
GTCAGTATTC CGTCTATCAC CAAATCCCGC CCTGAACCCG GTCGCTCCAT GAGCGCCAGC 
AGTGGCCTAT CTGCAAGGTC ATCTGCACAG AATGGCAGTG CGTTAAAGAG AGAATTCTCT 
GGAGGCAGCT ACAGTGCAAA GCGGCAGCCC ATGCCCTCCC CATCGGAGGG CTCGCTGTCC 
TCAGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCGCGGG ACTATGACGG AGAGGACTCT 
GATTCTCCGC GGCATTCCAC GGCTTCCAAC AGTTCCAACC TGAGCAGTCC TCCCAGCCCG 
GTGTCGCCCC GGAAGACCAA GAGCCTGTCT CTGGAGAGCA CAGACCGCGG GAGCTGGGAC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062438.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062500.1 Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X13, mRNA.

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CloneID	OBu18515
Clone ID Related Accession (Same CDS sequence)	XM_006062438.1 , XM_006062438.2
Accession Version	XM_006062438.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATAT TGAATAAATG GGAAATGCTG AAAAGAGCTG AGACAGCATG TTTTCGTGAA 
GAAAGGGATG TATTAGTGAA TGGAGACAAT AAATGGATTA CAACTTTGCA TTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTGCTTACT 
CTACTCAGCA AATTTGAAGA TAGATTACCT GAAGACATGG CTCGATTTTA CTTGGCTGAG 
ATGGTGATAG CAATTGATTC AGTTCATCAG CTGCATTATG TACACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGGTTAG CAGATTTTGG TTCATGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAATCC TCAGTGGCTG TTGGAACTCC AGATTATATC 
TCTCCTGAAA TCCTTCAAGC CATGGAAGAT GGAAAAGGCA GATATGGACC TGAGTGTGAC 
TGGTGGTCTT TGGGGGTCTG TATGTATGAA ATGCTTTATG GAGAAACACC ATTTTATGCA 
GAATCTCTGG TGGAAACATA TGGAAAAATC ATGAATCACA AAGAGAGGTT TCAGTTTCCT 
CCTCAAGTGA GTGATGTGTC TGAAAATGCT AAGGATCTTA TTCGAAGACT CATCTGTAGC 
AGAGAACATC GACTTGGTCA AAATGGAATA GAAGACTTTA AGAAGCACCC ATTTTTCAAT 
GGAATTGATT GGGATAATAT TCGAAACTGT GAAGCACCTT ATATTCCAGA AGTTAGTAGC 
CCAACAGATA CGTCGAATTT TGATGTGGAT GATGATTGTT TAAAGAATTC TGAAACGATG 
CCCCCACCAA CACATACTGC CTTTTCTGGT CACCATCTGC CATTTGTTGG TTTTACATAT 
ACCAGTAGCT GTGTTCTTTC TGATCGAAGC TGTTTAAGAG TTACAGCTGG TCCCACACCA 
TTGGATCTCG ATGTTAATGT GCAGAGGACT CTAGATAACA ACTTAGCAAC TGAAGCTTAT 
GAAAGAAGAA TTAAGCGCCT TGAGCAGGAA AAACTTGAAC TTAGTAGAAA GCTTCAAGAG 
TCAACGCAGA CTGTCCAGGC TCTGCAGTAT TCAACTGCTG ATGGTCCACT AACAGCAAGC 
AAAGATTTAG AAATAAAAAG CTTAAAAGAA GAAATTGAAA AACTAAGGAA ACAAGTAAGA 
GAATCAAGTC ACTTGGAACA GCAACTTGAA GAAGCTAATT CTGTGAGGCG AGAATTAGAT 
GATGCTTTTA GACAAATCAA GGCTTATGAA AAACAAATCA AGACATTACA ACAAGAAAGG 
GAAGAATTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGACG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGGCTAACAG AATTGCACAC CCAAAAACAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGTT GAAAGCTTAA GGCAAGAACT GCGCAGAACA 
GAAAGAGCCA AAAAAGAGCT GGAAGTTCGT ACAGAAGCTG TGGCTGCTGA AGCATCTAAA 
GACAGGAAAT TGCGTGAACA GAGTGAGCAC TATTCTAAGC AACTGGAGAA TGAACTAGAG 
GGACTGAAGC AAAAACAAAT TAGTTACTCA CCAGGCGTAT GCAGCATAGA ACATCAGCAA 
GAAATAACCA AACTAAAGAC TGATTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAATTG 
TCCAAGAGAG AAGGAATACA TGCAAATGAA ATTAAAAATC TGAAGAAAGA ACTACATGAT 
TCAGAAGGCC AACAACTTGC TCTCAACAAA GAAATTATGA TTTTGAAAGA CAAATTGGAA 
AAAAACAGGA GAGAGAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAGCAACAG 
TATGAACGAG AAAAAGTGTT ACTAACTGAA GAAAATAAAA AACTGACAAG TGAGCTTGAT 
AAGCTTACCA CTCTGTGTGA GAACTTAAGT GCACGCAACC AGCAGTTAGA AGAAGAGGTC 
AAAGATCTAG CAGATAAGAA GGAATCGGTT GCTCATTGGG AGGCTCAGAT CACAGAAATA 
ATTCAGTGGG TCAGTGATGA AAAGGATGCA CGAGGGTATC TTCAAGCCTT AGCTTCTAAA 
ATGACTGAAG AATTGGAAGC TTTAAGAAAC TCCAGCTTGG GGACCCGAGC AACAGATATG 
CCCTGGAAAA TGCGACGTTT TGCAAAACTG GATATGTCAG CCAGACTGGA GTTACAGTCA 
GCTCTGGATG CAGAAATAAG AGCCAAACAG GCCATCCAAG AAGAGCTGAA TAAAGTTAAA 
GCATCTAACA TCATAACAGA ATGTAAACTA AAAGATTCAG AGAAGAAGAA CTTGGAACTA 
CTATCAGAAA TCGAACAACT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GATCAATTTG AAGATTCCTT TTCTTCTTCC TCATCTTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCAT CCTGTACTCC AGCTAGCAAA GGCAGACGTA CTGATCCCGT TGAGAACACA 
TATGTACGGA ATCCGAACGT CAAGTTTTAC ATCCAGTCAA GGTCCACCTC TCCTTCCACA 
TCTAGTGAAG CTGAGCCAGT TAAGACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGCTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAGGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAATGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG CAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCGTA CAACGTCCAG TGGATGGCGA TCTTCAGTGA GCAGCTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAACGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CGGATAATAG CCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCGGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGCATT CAGTGGCTCG 
GTCAGTATTC CGTCTATCAC CAAATCCCGC CCTGAACCCG GTCGCTCCAT GAGCGCCAGC 
AGTGGCCTAT CTGCAAGGTC ATCTGCACAG AATGGCAGTG CGTTAAAGAG AGAATTCTCT 
GGAGGCAGCT ACAGTGCAAA GCGGCAGCCC ATGCCCTCCC CATCGGAGGG CTCGCTGTCC 
TCAGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCGCGGG ACTATGACGG AGAGGACTCT 
GATTCTCCGC GGCATTCCAC GGCTTCCAAC AGTTCCAACC TGAGCAGTCC TCCCAGCCCG 
GTGTCGCCCC GGAAGACCAA GAGCCTGTCT CTGGAGAGCA CAGACCGCGG GAGCTGGGAC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062500.1
CDS	283..5508
Translation	MKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062438.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062438.1

ORF Insert Sequence:

ATGAAAATAT TGAATAAATG GGAAATGCTG AAAAGAGCTG AGACAGCATG TTTTCGTGAA 
GAAAGGGATG TATTAGTGAA TGGAGACAAT AAATGGATTA CAACTTTGCA TTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTGCTTACT 
CTACTCAGCA AATTTGAAGA TAGATTACCT GAAGACATGG CTCGATTTTA CTTGGCTGAG 
ATGGTGATAG CAATTGATTC AGTTCATCAG CTGCATTATG TACACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGGTTAG CAGATTTTGG TTCATGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAATCC TCAGTGGCTG TTGGAACTCC AGATTATATC 
TCTCCTGAAA TCCTTCAAGC CATGGAAGAT GGAAAAGGCA GATATGGACC TGAGTGTGAC 
TGGTGGTCTT TGGGGGTCTG TATGTATGAA ATGCTTTATG GAGAAACACC ATTTTATGCA 
GAATCTCTGG TGGAAACATA TGGAAAAATC ATGAATCACA AAGAGAGGTT TCAGTTTCCT 
CCTCAAGTGA GTGATGTGTC TGAAAATGCT AAGGATCTTA TTCGAAGACT CATCTGTAGC 
AGAGAACATC GACTTGGTCA AAATGGAATA GAAGACTTTA AGAAGCACCC ATTTTTCAAT 
GGAATTGATT GGGATAATAT TCGAAACTGT GAAGCACCTT ATATTCCAGA AGTTAGTAGC 
CCAACAGATA CGTCGAATTT TGATGTGGAT GATGATTGTT TAAAGAATTC TGAAACGATG 
CCCCCACCAA CACATACTGC CTTTTCTGGT CACCATCTGC CATTTGTTGG TTTTACATAT 
ACCAGTAGCT GTGTTCTTTC TGATCGAAGC TGTTTAAGAG TTACAGCTGG TCCCACACCA 
TTGGATCTCG ATGTTAATGT GCAGAGGACT CTAGATAACA ACTTAGCAAC TGAAGCTTAT 
GAAAGAAGAA TTAAGCGCCT TGAGCAGGAA AAACTTGAAC TTAGTAGAAA GCTTCAAGAG 
TCAACGCAGA CTGTCCAGGC TCTGCAGTAT TCAACTGCTG ATGGTCCACT AACAGCAAGC 
AAAGATTTAG AAATAAAAAG CTTAAAAGAA GAAATTGAAA AACTAAGGAA ACAAGTAAGA 
GAATCAAGTC ACTTGGAACA GCAACTTGAA GAAGCTAATT CTGTGAGGCG AGAATTAGAT 
GATGCTTTTA GACAAATCAA GGCTTATGAA AAACAAATCA AGACATTACA ACAAGAAAGG 
GAAGAATTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGACG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGGCTAACAG AATTGCACAC CCAAAAACAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGTT GAAAGCTTAA GGCAAGAACT GCGCAGAACA 
GAAAGAGCCA AAAAAGAGCT GGAAGTTCGT ACAGAAGCTG TGGCTGCTGA AGCATCTAAA 
GACAGGAAAT TGCGTGAACA GAGTGAGCAC TATTCTAAGC AACTGGAGAA TGAACTAGAG 
GGACTGAAGC AAAAACAAAT TAGTTACTCA CCAGGCGTAT GCAGCATAGA ACATCAGCAA 
GAAATAACCA AACTAAAGAC TGATTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAATTG 
TCCAAGAGAG AAGGAATACA TGCAAATGAA ATTAAAAATC TGAAGAAAGA ACTACATGAT 
TCAGAAGGCC AACAACTTGC TCTCAACAAA GAAATTATGA TTTTGAAAGA CAAATTGGAA 
AAAAACAGGA GAGAGAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAGCAACAG 
TATGAACGAG AAAAAGTGTT ACTAACTGAA GAAAATAAAA AACTGACAAG TGAGCTTGAT 
AAGCTTACCA CTCTGTGTGA GAACTTAAGT GCACGCAACC AGCAGTTAGA AGAAGAGGTC 
AAAGATCTAG CAGATAAGAA GGAATCGGTT GCTCATTGGG AGGCTCAGAT CACAGAAATA 
ATTCAGTGGG TCAGTGATGA AAAGGATGCA CGAGGGTATC TTCAAGCCTT AGCTTCTAAA 
ATGACTGAAG AATTGGAAGC TTTAAGAAAC TCCAGCTTGG GGACCCGAGC AACAGATATG 
CCCTGGAAAA TGCGACGTTT TGCAAAACTG GATATGTCAG CCAGACTGGA GTTACAGTCA 
GCTCTGGATG CAGAAATAAG AGCCAAACAG GCCATCCAAG AAGAGCTGAA TAAAGTTAAA 
GCATCTAACA TCATAACAGA ATGTAAACTA AAAGATTCAG AGAAGAAGAA CTTGGAACTA 
CTATCAGAAA TCGAACAACT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GATCAATTTG AAGATTCCTT TTCTTCTTCC TCATCTTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCAT CCTGTACTCC AGCTAGCAAA GGCAGACGTA CTGATCCCGT TGAGAACACA 
TATGTACGGA ATCCGAACGT CAAGTTTTAC ATCCAGTCAA GGTCCACCTC TCCTTCCACA 
TCTAGTGAAG CTGAGCCAGT TAAGACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGCTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAGGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAATGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG CAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCGTA CAACGTCCAG TGGATGGCGA TCTTCAGTGA GCAGCTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAACGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CGGATAATAG CCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCGGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGCATT CAGTGGCTCG 
GTCAGTATTC CGTCTATCAC CAAATCCCGC CCTGAACCCG GTCGCTCCAT GAGCGCCAGC 
AGTGGCCTAT CTGCAAGGTC ATCTGCACAG AATGGCAGTG CGTTAAAGAG AGAATTCTCT 
GGAGGCAGCT ACAGTGCAAA GCGGCAGCCC ATGCCCTCCC CATCGGAGGG CTCGCTGTCC 
TCAGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCGCGGG ACTATGACGG AGAGGACTCT 
GATTCTCCGC GGCATTCCAC GGCTTCCAAC AGTTCCAACC TGAGCAGTCC TCCCAGCCCG 
GTGTCGCCCC GGAAGACCAA GAGCCTGTCT CTGGAGAGCA CAGACCGCGG GAGCTGGGAC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062438.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062500.1 Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X12, mRNA.

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CloneID	OBu18515
Clone ID Related Accession (Same CDS sequence)	XM_006062438.1 , XM_006062438.2
Accession Version	XM_006062438.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006062438.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATAT TGAATAAATG GGAAATGCTG AAAAGAGCTG AGACAGCATG TTTTCGTGAA 
GAAAGGGATG TATTAGTGAA TGGAGACAAT AAATGGATTA CAACTTTGCA TTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTGCTTACT 
CTACTCAGCA AATTTGAAGA TAGATTACCT GAAGACATGG CTCGATTTTA CTTGGCTGAG 
ATGGTGATAG CAATTGATTC AGTTCATCAG CTGCATTATG TACACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGGTTAG CAGATTTTGG TTCATGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAATCC TCAGTGGCTG TTGGAACTCC AGATTATATC 
TCTCCTGAAA TCCTTCAAGC CATGGAAGAT GGAAAAGGCA GATATGGACC TGAGTGTGAC 
TGGTGGTCTT TGGGGGTCTG TATGTATGAA ATGCTTTATG GAGAAACACC ATTTTATGCA 
GAATCTCTGG TGGAAACATA TGGAAAAATC ATGAATCACA AAGAGAGGTT TCAGTTTCCT 
CCTCAAGTGA GTGATGTGTC TGAAAATGCT AAGGATCTTA TTCGAAGACT CATCTGTAGC 
AGAGAACATC GACTTGGTCA AAATGGAATA GAAGACTTTA AGAAGCACCC ATTTTTCAAT 
GGAATTGATT GGGATAATAT TCGAAACTGT GAAGCACCTT ATATTCCAGA AGTTAGTAGC 
CCAACAGATA CGTCGAATTT TGATGTGGAT GATGATTGTT TAAAGAATTC TGAAACGATG 
CCCCCACCAA CACATACTGC CTTTTCTGGT CACCATCTGC CATTTGTTGG TTTTACATAT 
ACCAGTAGCT GTGTTCTTTC TGATCGAAGC TGTTTAAGAG TTACAGCTGG TCCCACACCA 
TTGGATCTCG ATGTTAATGT GCAGAGGACT CTAGATAACA ACTTAGCAAC TGAAGCTTAT 
GAAAGAAGAA TTAAGCGCCT TGAGCAGGAA AAACTTGAAC TTAGTAGAAA GCTTCAAGAG 
TCAACGCAGA CTGTCCAGGC TCTGCAGTAT TCAACTGCTG ATGGTCCACT AACAGCAAGC 
AAAGATTTAG AAATAAAAAG CTTAAAAGAA GAAATTGAAA AACTAAGGAA ACAAGTAAGA 
GAATCAAGTC ACTTGGAACA GCAACTTGAA GAAGCTAATT CTGTGAGGCG AGAATTAGAT 
GATGCTTTTA GACAAATCAA GGCTTATGAA AAACAAATCA AGACATTACA ACAAGAAAGG 
GAAGAATTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGACG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGGCTAACAG AATTGCACAC CCAAAAACAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGTT GAAAGCTTAA GGCAAGAACT GCGCAGAACA 
GAAAGAGCCA AAAAAGAGCT GGAAGTTCGT ACAGAAGCTG TGGCTGCTGA AGCATCTAAA 
GACAGGAAAT TGCGTGAACA GAGTGAGCAC TATTCTAAGC AACTGGAGAA TGAACTAGAG 
GGACTGAAGC AAAAACAAAT TAGTTACTCA CCAGGCGTAT GCAGCATAGA ACATCAGCAA 
GAAATAACCA AACTAAAGAC TGATTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAATTG 
TCCAAGAGAG AAGGAATACA TGCAAATGAA ATTAAAAATC TGAAGAAAGA ACTACATGAT 
TCAGAAGGCC AACAACTTGC TCTCAACAAA GAAATTATGA TTTTGAAAGA CAAATTGGAA 
AAAAACAGGA GAGAGAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAGCAACAG 
TATGAACGAG AAAAAGTGTT ACTAACTGAA GAAAATAAAA AACTGACAAG TGAGCTTGAT 
AAGCTTACCA CTCTGTGTGA GAACTTAAGT GCACGCAACC AGCAGTTAGA AGAAGAGGTC 
AAAGATCTAG CAGATAAGAA GGAATCGGTT GCTCATTGGG AGGCTCAGAT CACAGAAATA 
ATTCAGTGGG TCAGTGATGA AAAGGATGCA CGAGGGTATC TTCAAGCCTT AGCTTCTAAA 
ATGACTGAAG AATTGGAAGC TTTAAGAAAC TCCAGCTTGG GGACCCGAGC AACAGATATG 
CCCTGGAAAA TGCGACGTTT TGCAAAACTG GATATGTCAG CCAGACTGGA GTTACAGTCA 
GCTCTGGATG CAGAAATAAG AGCCAAACAG GCCATCCAAG AAGAGCTGAA TAAAGTTAAA 
GCATCTAACA TCATAACAGA ATGTAAACTA AAAGATTCAG AGAAGAAGAA CTTGGAACTA 
CTATCAGAAA TCGAACAACT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GATCAATTTG AAGATTCCTT TTCTTCTTCC TCATCTTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCAT CCTGTACTCC AGCTAGCAAA GGCAGACGTA CTGATCCCGT TGAGAACACA 
TATGTACGGA ATCCGAACGT CAAGTTTTAC ATCCAGTCAA GGTCCACCTC TCCTTCCACA 
TCTAGTGAAG CTGAGCCAGT TAAGACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGCTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAGGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAATGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG CAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCGTA CAACGTCCAG TGGATGGCGA TCTTCAGTGA GCAGCTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAACGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CGGATAATAG CCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCGGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGCATT CAGTGGCTCG 
GTCAGTATTC CGTCTATCAC CAAATCCCGC CCTGAACCCG GTCGCTCCAT GAGCGCCAGC 
AGTGGCCTAT CTGCAAGGTC ATCTGCACAG AATGGCAGTG CGTTAAAGAG AGAATTCTCT 
GGAGGCAGCT ACAGTGCAAA GCGGCAGCCC ATGCCCTCCC CATCGGAGGG CTCGCTGTCC 
TCAGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCGCGGG ACTATGACGG AGAGGACTCT 
GATTCTCCGC GGCATTCCAC GGCTTCCAAC AGTTCCAACC TGAGCAGTCC TCCCAGCCCG 
GTGTCGCCCC GGAAGACCAA GAGCCTGTCT CTGGAGAGCA CAGACCGCGG GAGCTGGGAC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062500.1
CDS	293..5518
Translation	MKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062438.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062438.2

ORF Insert Sequence:

ATGAAAATAT TGAATAAATG GGAAATGCTG AAAAGAGCTG AGACAGCATG TTTTCGTGAA 
GAAAGGGATG TATTAGTGAA TGGAGACAAT AAATGGATTA CAACTTTGCA TTATGCTTTC 
CAGGATGACA ATAACTTATA CCTGGTTATG GATTATTATG TTGGTGGGGA TTTGCTTACT 
CTACTCAGCA AATTTGAAGA TAGATTACCT GAAGACATGG CTCGATTTTA CTTGGCTGAG 
ATGGTGATAG CAATTGATTC AGTTCATCAG CTGCATTATG TACACAGAGA CATCAAACCT 
GACAATATAT TGATGGATAT GAATGGACAT ATTCGGTTAG CAGATTTTGG TTCATGTCTG 
AAGCTGATGG AAGATGGAAC GGTCCAATCC TCAGTGGCTG TTGGAACTCC AGATTATATC 
TCTCCTGAAA TCCTTCAAGC CATGGAAGAT GGAAAAGGCA GATATGGACC TGAGTGTGAC 
TGGTGGTCTT TGGGGGTCTG TATGTATGAA ATGCTTTATG GAGAAACACC ATTTTATGCA 
GAATCTCTGG TGGAAACATA TGGAAAAATC ATGAATCACA AAGAGAGGTT TCAGTTTCCT 
CCTCAAGTGA GTGATGTGTC TGAAAATGCT AAGGATCTTA TTCGAAGACT CATCTGTAGC 
AGAGAACATC GACTTGGTCA AAATGGAATA GAAGACTTTA AGAAGCACCC ATTTTTCAAT 
GGAATTGATT GGGATAATAT TCGAAACTGT GAAGCACCTT ATATTCCAGA AGTTAGTAGC 
CCAACAGATA CGTCGAATTT TGATGTGGAT GATGATTGTT TAAAGAATTC TGAAACGATG 
CCCCCACCAA CACATACTGC CTTTTCTGGT CACCATCTGC CATTTGTTGG TTTTACATAT 
ACCAGTAGCT GTGTTCTTTC TGATCGAAGC TGTTTAAGAG TTACAGCTGG TCCCACACCA 
TTGGATCTCG ATGTTAATGT GCAGAGGACT CTAGATAACA ACTTAGCAAC TGAAGCTTAT 
GAAAGAAGAA TTAAGCGCCT TGAGCAGGAA AAACTTGAAC TTAGTAGAAA GCTTCAAGAG 
TCAACGCAGA CTGTCCAGGC TCTGCAGTAT TCAACTGCTG ATGGTCCACT AACAGCAAGC 
AAAGATTTAG AAATAAAAAG CTTAAAAGAA GAAATTGAAA AACTAAGGAA ACAAGTAAGA 
GAATCAAGTC ACTTGGAACA GCAACTTGAA GAAGCTAATT CTGTGAGGCG AGAATTAGAT 
GATGCTTTTA GACAAATCAA GGCTTATGAA AAACAAATCA AGACATTACA ACAAGAAAGG 
GAAGAATTAA ATAAGGAACT AGTCCAGGCT AGTGAGCGAT TAAAAAACCA ATCCAAAGAG 
CTGAAAGACG CACACTGTCA GAGGAAACTG GCCATGCAGG AATTCATGGA GATCAATGAG 
CGGCTAACAG AATTGCACAC CCAAAAACAG AAACTTGCTC GCCATGTCCG AGATAAGGAA 
GAAGAGGTGG ACCTGGTGAT GCAAAAAGTT GAAAGCTTAA GGCAAGAACT GCGCAGAACA 
GAAAGAGCCA AAAAAGAGCT GGAAGTTCGT ACAGAAGCTG TGGCTGCTGA AGCATCTAAA 
GACAGGAAAT TGCGTGAACA GAGTGAGCAC TATTCTAAGC AACTGGAGAA TGAACTAGAG 
GGACTGAAGC AAAAACAAAT TAGTTACTCA CCAGGCGTAT GCAGCATAGA ACATCAGCAA 
GAAATAACCA AACTAAAGAC TGATTTGGAA AAGAAAAGTA TCTTTTATGA AGAAGAATTG 
TCCAAGAGAG AAGGAATACA TGCAAATGAA ATTAAAAATC TGAAGAAAGA ACTACATGAT 
TCAGAAGGCC AACAACTTGC TCTCAACAAA GAAATTATGA TTTTGAAAGA CAAATTGGAA 
AAAAACAGGA GAGAGAGTCA AAGTGAAAGG GAAGAATTTG AAAATGAGTT CAAGCAACAG 
TATGAACGAG AAAAAGTGTT ACTAACTGAA GAAAATAAAA AACTGACAAG TGAGCTTGAT 
AAGCTTACCA CTCTGTGTGA GAACTTAAGT GCACGCAACC AGCAGTTAGA AGAAGAGGTC 
AAAGATCTAG CAGATAAGAA GGAATCGGTT GCTCATTGGG AGGCTCAGAT CACAGAAATA 
ATTCAGTGGG TCAGTGATGA AAAGGATGCA CGAGGGTATC TTCAAGCCTT AGCTTCTAAA 
ATGACTGAAG AATTGGAAGC TTTAAGAAAC TCCAGCTTGG GGACCCGAGC AACAGATATG 
CCCTGGAAAA TGCGACGTTT TGCAAAACTG GATATGTCAG CCAGACTGGA GTTACAGTCA 
GCTCTGGATG CAGAAATAAG AGCCAAACAG GCCATCCAAG AAGAGCTGAA TAAAGTTAAA 
GCATCTAACA TCATAACAGA ATGTAAACTA AAAGATTCAG AGAAGAAGAA CTTGGAACTA 
CTATCAGAAA TCGAACAACT GATAAAGGAC ACTGAAGAGC TTAGATCTGA AAAGGGTATA 
GAGCACCAAG ACTCACAGCA TTCTTTCTTG GCATTTTTGA ATACGCCTAC CGATGCTCTG 
GATCAATTTG AAGATTCCTT TTCTTCTTCC TCATCTTCAC TGATTGATTT TTTGGATGAC 
CGCTCTCCAT CCTGTACTCC AGCTAGCAAA GGCAGACGTA CTGATCCCGT TGAGAACACA 
TATGTACGGA ATCCGAACGT CAAGTTTTAC ATCCAGTCAA GGTCCACCTC TCCTTCCACA 
TCTAGTGAAG CTGAGCCAGT TAAGACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGCTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAGGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAATGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG CAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCGTA CAACGTCCAG TGGATGGCGA TCTTCAGTGA GCAGCTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAACGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CGGATAATAG CCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGCCGGGTTT CCCTTATCCA TCCCCTCATC ATCACTCCGG TCTGATCTCC 
TCTCCGATCA ACTTTGAGCA CATCTATCAC ATGACTGTTA ATTCTGCTGA AAAATTTCTC 
TCTCCTGATT CCATAAACCC TGAATATTCC CCGTCTCTGC GCTCTGTCCC TGGTACACCA 
AGCTTTATGA CTCTGAGGAA CCCTCGGCCT CAGGAAAGTC GGACAGCATT CAGTGGCTCG 
GTCAGTATTC CGTCTATCAC CAAATCCCGC CCTGAACCCG GTCGCTCCAT GAGCGCCAGC 
AGTGGCCTAT CTGCAAGGTC ATCTGCACAG AATGGCAGTG CGTTAAAGAG AGAATTCTCT 
GGAGGCAGCT ACAGTGCAAA GCGGCAGCCC ATGCCCTCCC CATCGGAGGG CTCGCTGTCC 
TCAGGAGGCA TGGACCAGGG AAGTGACGCC CCAGCGCGGG ACTATGACGG AGAGGACTCT 
GATTCTCCGC GGCATTCCAC GGCTTCCAAC AGTTCCAACC TGAGCAGTCC TCCCAGCCCG 
GTGTCGCCCC GGAAGACCAA GAGCCTGTCT CTGGAGAGCA CAGACCGCGG GAGCTGGGAC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062439.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062501.1 Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBu18516
Clone ID Related Accession (Same CDS sequence)	XM_006062439.1 , XM_006062439.2
Accession Version	XM_006062439.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCTACTCC ACTTCCAGTT CATACACCAA CCTTAAGGAA AAAGGGATGC 
CCTGGCTCAA CTGGCTTTCC ATCGAAGCGC AAGATTCATC AGTTTTTTGT CAAATCTTTT 
ACTACTCCCA CCAGGTGTCA TCAGTGTACA TCTTTGATGG TGGGTTTGAT AAGACAAGGC 
TGTTCTTGTG AAGTGTGTGG ATTTTCATGT CACATAACTT GTGTAAACAA AGCTCCAACT 
GCTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCTCTGG GTATAGATCC ACAGAAAGGA 
ATAGGAACAG CATATGAAGG GCATGTCAGG ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA 
TGGCAAAGAG CACTGGCTGT AGTCTGTGAC TTCAAGCTCT TTCTGTACGA TATTGCCGAA 
GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC 
TGTATATTTA GAGTCACAGC TTCCCAGCTC TCAGCATCTA GTAACAAATG TTCCATCCTG 
ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT AAATGGGTGG GAGTACTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCATGAAAGA 
ATAGCTTTGG GGAATGAAGA AGGGTTATTT GTTGTGCATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATAGAACTCA TTCCAAATGA TCAACTTGTT 
GCCGTGATCT CAGGACGAAA TCGACACGTC CGACTTTTTC CTATGTCAGC TCTGGACGGG 
CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGCT GTCAAACAAT AACTTCTGGC 
AAAGTGCGTC ATGGAGCTCT TACATGCTTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACGCGTCAC AGAAAATTTA AGGAAATTCA AGTCCCGTAC 
AACGTCCAGT GGATGGCGAT CTTCAGTGAG CAGCTCTGTG TGGGATTCCA GTCTGGATTT 
CTAAGATATC CCCTGAATGG AGAAGGAAGT CCATACAGTA TGCTCCATTC AAATGACAAT 
ACACTCTCAT TTATTGCACA TCAACCAATG GATGCCATCT GTGCGGTTGA AATCTCCAGT 
AAAGAATACC TGCTGTGTTT TAACAGCATT GGAATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAACTGAT GTGGCCAGCG AATCCTTCCT CTTGTTGTTA CAATGCACCG 
TATCTCTCAG TGTACAGTGA AAACGCAGTT GACATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TCCCACTCAA AAAGGTTCGC CCCTTAAACA GTGAAGGATC ATTAAATCTT 
TTAGGCTTGG AGACGATTAG ATTGATATAT TTCAAAAATA AAATGGCAGA AGGGGATGAA 
CTGGTAGTTC CTGAAACATC GGATAATAGC CGGAAGCAAA TGGTTAGAAA TATTAACAAT 
AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA GAGGAAAGAA TGCAACAGAG GAGGGAGATG 
TTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACATATGG GTCCAGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GAACCCTCGG 
CCTCAGGAAA GTCGGACAGC ATTCAGTGGC TCGGTCAGTA TTCCGTCTAT CACCAAATCC 
CGCCCTGAAC CCGGTCGCTC CATGAGCGCC AGCAGTGGCC TATCTGCAAG GTCATCTGCA 
CAGAATGGCA GTGCGTTAAA GAGAGAATTC TCTGGAGGCA GCTACAGTGC AAAGCGGCAG 
CCCATGCCCT CCCCATCGGA GGGCTCGCTG TCCTCAGGAG GCATGGACCA GGGAAGTGAC 
GCCCCAGCGC GGGACTATGA CGGAGAGGAC TCTGATTCTC CGCGGCATTC CACGGCTTCC 
AACAGTTCCA ACCTGAGCAG TCCTCCCAGC CCGGTGTCGC CCCGGAAGAC CAAGAGCCTG 
TCTCTGGAGA GCACAGACCG CGGGAGCTGG GACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062501.1
CDS	286..5484
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062439.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062439.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCTACTCC ACTTCCAGTT CATACACCAA CCTTAAGGAA AAAGGGATGC 
CCTGGCTCAA CTGGCTTTCC ATCGAAGCGC AAGATTCATC AGTTTTTTGT CAAATCTTTT 
ACTACTCCCA CCAGGTGTCA TCAGTGTACA TCTTTGATGG TGGGTTTGAT AAGACAAGGC 
TGTTCTTGTG AAGTGTGTGG ATTTTCATGT CACATAACTT GTGTAAACAA AGCTCCAACT 
GCTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCTCTGG GTATAGATCC ACAGAAAGGA 
ATAGGAACAG CATATGAAGG GCATGTCAGG ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA 
TGGCAAAGAG CACTGGCTGT AGTCTGTGAC TTCAAGCTCT TTCTGTACGA TATTGCCGAA 
GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC 
TGTATATTTA GAGTCACAGC TTCCCAGCTC TCAGCATCTA GTAACAAATG TTCCATCCTG 
ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT AAATGGGTGG GAGTACTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCATGAAAGA 
ATAGCTTTGG GGAATGAAGA AGGGTTATTT GTTGTGCATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATAGAACTCA TTCCAAATGA TCAACTTGTT 
GCCGTGATCT CAGGACGAAA TCGACACGTC CGACTTTTTC CTATGTCAGC TCTGGACGGG 
CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGCT GTCAAACAAT AACTTCTGGC 
AAAGTGCGTC ATGGAGCTCT TACATGCTTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACGCGTCAC AGAAAATTTA AGGAAATTCA AGTCCCGTAC 
AACGTCCAGT GGATGGCGAT CTTCAGTGAG CAGCTCTGTG TGGGATTCCA GTCTGGATTT 
CTAAGATATC CCCTGAATGG AGAAGGAAGT CCATACAGTA TGCTCCATTC AAATGACAAT 
ACACTCTCAT TTATTGCACA TCAACCAATG GATGCCATCT GTGCGGTTGA AATCTCCAGT 
AAAGAATACC TGCTGTGTTT TAACAGCATT GGAATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAACTGAT GTGGCCAGCG AATCCTTCCT CTTGTTGTTA CAATGCACCG 
TATCTCTCAG TGTACAGTGA AAACGCAGTT GACATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TCCCACTCAA AAAGGTTCGC CCCTTAAACA GTGAAGGATC ATTAAATCTT 
TTAGGCTTGG AGACGATTAG ATTGATATAT TTCAAAAATA AAATGGCAGA AGGGGATGAA 
CTGGTAGTTC CTGAAACATC GGATAATAGC CGGAAGCAAA TGGTTAGAAA TATTAACAAT 
AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA GAGGAAAGAA TGCAACAGAG GAGGGAGATG 
TTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACATATGG GTCCAGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GAACCCTCGG 
CCTCAGGAAA GTCGGACAGC ATTCAGTGGC TCGGTCAGTA TTCCGTCTAT CACCAAATCC 
CGCCCTGAAC CCGGTCGCTC CATGAGCGCC AGCAGTGGCC TATCTGCAAG GTCATCTGCA 
CAGAATGGCA GTGCGTTAAA GAGAGAATTC TCTGGAGGCA GCTACAGTGC AAAGCGGCAG 
CCCATGCCCT CCCCATCGGA GGGCTCGCTG TCCTCAGGAG GCATGGACCA GGGAAGTGAC 
GCCCCAGCGC GGGACTATGA CGGAGAGGAC TCTGATTCTC CGCGGCATTC CACGGCTTCC 
AACAGTTCCA ACCTGAGCAG TCCTCCCAGC CCGGTGTCGC CCCGGAAGAC CAAGAGCCTG 
TCTCTGGAGA GCACAGACCG CGGGAGCTGG GACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062439.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062501.1 Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X13, mRNA.

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CloneID	OBu18516
Clone ID Related Accession (Same CDS sequence)	XM_006062439.1 , XM_006062439.2
Accession Version	XM_006062439.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006062439.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCTACTCC ACTTCCAGTT CATACACCAA CCTTAAGGAA AAAGGGATGC 
CCTGGCTCAA CTGGCTTTCC ATCGAAGCGC AAGATTCATC AGTTTTTTGT CAAATCTTTT 
ACTACTCCCA CCAGGTGTCA TCAGTGTACA TCTTTGATGG TGGGTTTGAT AAGACAAGGC 
TGTTCTTGTG AAGTGTGTGG ATTTTCATGT CACATAACTT GTGTAAACAA AGCTCCAACT 
GCTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCTCTGG GTATAGATCC ACAGAAAGGA 
ATAGGAACAG CATATGAAGG GCATGTCAGG ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA 
TGGCAAAGAG CACTGGCTGT AGTCTGTGAC TTCAAGCTCT TTCTGTACGA TATTGCCGAA 
GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC 
TGTATATTTA GAGTCACAGC TTCCCAGCTC TCAGCATCTA GTAACAAATG TTCCATCCTG 
ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT AAATGGGTGG GAGTACTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCATGAAAGA 
ATAGCTTTGG GGAATGAAGA AGGGTTATTT GTTGTGCATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATAGAACTCA TTCCAAATGA TCAACTTGTT 
GCCGTGATCT CAGGACGAAA TCGACACGTC CGACTTTTTC CTATGTCAGC TCTGGACGGG 
CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGCT GTCAAACAAT AACTTCTGGC 
AAAGTGCGTC ATGGAGCTCT TACATGCTTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACGCGTCAC AGAAAATTTA AGGAAATTCA AGTCCCGTAC 
AACGTCCAGT GGATGGCGAT CTTCAGTGAG CAGCTCTGTG TGGGATTCCA GTCTGGATTT 
CTAAGATATC CCCTGAATGG AGAAGGAAGT CCATACAGTA TGCTCCATTC AAATGACAAT 
ACACTCTCAT TTATTGCACA TCAACCAATG GATGCCATCT GTGCGGTTGA AATCTCCAGT 
AAAGAATACC TGCTGTGTTT TAACAGCATT GGAATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAACTGAT GTGGCCAGCG AATCCTTCCT CTTGTTGTTA CAATGCACCG 
TATCTCTCAG TGTACAGTGA AAACGCAGTT GACATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TCCCACTCAA AAAGGTTCGC CCCTTAAACA GTGAAGGATC ATTAAATCTT 
TTAGGCTTGG AGACGATTAG ATTGATATAT TTCAAAAATA AAATGGCAGA AGGGGATGAA 
CTGGTAGTTC CTGAAACATC GGATAATAGC CGGAAGCAAA TGGTTAGAAA TATTAACAAT 
AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA GAGGAAAGAA TGCAACAGAG GAGGGAGATG 
TTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACATATGG GTCCAGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GAACCCTCGG 
CCTCAGGAAA GTCGGACAGC ATTCAGTGGC TCGGTCAGTA TTCCGTCTAT CACCAAATCC 
CGCCCTGAAC CCGGTCGCTC CATGAGCGCC AGCAGTGGCC TATCTGCAAG GTCATCTGCA 
CAGAATGGCA GTGCGTTAAA GAGAGAATTC TCTGGAGGCA GCTACAGTGC AAAGCGGCAG 
CCCATGCCCT CCCCATCGGA GGGCTCGCTG TCCTCAGGAG GCATGGACCA GGGAAGTGAC 
GCCCCAGCGC GGGACTATGA CGGAGAGGAC TCTGATTCTC CGCGGCATTC CACGGCTTCC 
AACAGTTCCA ACCTGAGCAG TCCTCCCAGC CCGGTGTCGC CCCGGAAGAC CAAGAGCCTG 
TCTCTGGAGA GCACAGACCG CGGGAGCTGG GACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062501.1
CDS	766..5964
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062439.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062439.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAACGCTCT CCATCCTGTA CTCCAGCTAG CAAAGGCAGA 
CGTACTGTAG ACTCTACTCC ACTTCCAGTT CATACACCAA CCTTAAGGAA AAAGGGATGC 
CCTGGCTCAA CTGGCTTTCC ATCGAAGCGC AAGATTCATC AGTTTTTTGT CAAATCTTTT 
ACTACTCCCA CCAGGTGTCA TCAGTGTACA TCTTTGATGG TGGGTTTGAT AAGACAAGGC 
TGTTCTTGTG AAGTGTGTGG ATTTTCATGT CACATAACTT GTGTAAACAA AGCTCCAACT 
GCTTGTCCAG TTCCTCCTGA ACAGACAAAA GGTCCTCTGG GTATAGATCC ACAGAAAGGA 
ATAGGAACAG CATATGAAGG GCATGTCAGG ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA 
TGGCAAAGAG CACTGGCTGT AGTCTGTGAC TTCAAGCTCT TTCTGTACGA TATTGCCGAA 
GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC AGTCAAGTGA TTGACATGAG GGATGAAGAA 
TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT GTTATTCATG CAAGTCGAAA AGATATACCC 
TGTATATTTA GAGTCACAGC TTCCCAGCTC TCAGCATCTA GTAACAAATG TTCCATCCTG 
ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT AAATGGGTGG GAGTACTGAG TGAATTGCAC 
AAGATTTTGA AGAAAAACAA ATTCAGAGAC CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT 
GACAGCACTC TGCCCCTCAT TAAAACAACC CAGGCAGCTG CAATCATAGA TCATGAAAGA 
ATAGCTTTGG GGAATGAAGA AGGGTTATTT GTTGTGCATG TCACCAAAGA TGAAATTATT 
AGAGTTGGTG ACAATAAGAA GATTCATCAG ATAGAACTCA TTCCAAATGA TCAACTTGTT 
GCCGTGATCT CAGGACGAAA TCGACACGTC CGACTTTTTC CTATGTCAGC TCTGGACGGG 
CGAGAGACTG ATTTTTATAA GCTGGCAGAA ACTAAAGGCT GTCAAACAAT AACTTCTGGC 
AAAGTGCGTC ATGGAGCTCT TACATGCTTG TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT 
TATGAACTAT TTCAGAGCAA GACGCGTCAC AGAAAATTTA AGGAAATTCA AGTCCCGTAC 
AACGTCCAGT GGATGGCGAT CTTCAGTGAG CAGCTCTGTG TGGGATTCCA GTCTGGATTT 
CTAAGATATC CCCTGAATGG AGAAGGAAGT CCATACAGTA TGCTCCATTC AAATGACAAT 
ACACTCTCAT TTATTGCACA TCAACCAATG GATGCCATCT GTGCGGTTGA AATCTCCAGT 
AAAGAATACC TGCTGTGTTT TAACAGCATT GGAATATACA CTGACTGCCA GGGCCGAAGA 
TCTAGACAAC AGGAACTGAT GTGGCCAGCG AATCCTTCCT CTTGTTGTTA CAATGCACCG 
TATCTCTCAG TGTACAGTGA AAACGCAGTT GACATCTTTG ATGTGAACTC CATGGAATGG 
ATTCAGACTC TCCCACTCAA AAAGGTTCGC CCCTTAAACA GTGAAGGATC ATTAAATCTT 
TTAGGCTTGG AGACGATTAG ATTGATATAT TTCAAAAATA AAATGGCAGA AGGGGATGAA 
CTGGTAGTTC CTGAAACATC GGATAATAGC CGGAAGCAAA TGGTTAGAAA TATTAACAAT 
AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA GAGGAAAGAA TGCAACAGAG GAGGGAGATG 
TTACGAGATC CAGAAATGAG AAATAAATTA ATTTCTAACC CAACTAATTT TAACCACATA 
GCACATATGG GTCCAGGAGA TGGAATACAG ATCCTAAAAG ACCTGCCCAT GAACCCTCGG 
CCTCAGGAAA GTCGGACAGC ATTCAGTGGC TCGGTCAGTA TTCCGTCTAT CACCAAATCC 
CGCCCTGAAC CCGGTCGCTC CATGAGCGCC AGCAGTGGCC TATCTGCAAG GTCATCTGCA 
CAGAATGGCA GTGCGTTAAA GAGAGAATTC TCTGGAGGCA GCTACAGTGC AAAGCGGCAG 
CCCATGCCCT CCCCATCGGA GGGCTCGCTG TCCTCAGGAG GCATGGACCA GGGAAGTGAC 
GCCCCAGCGC GGGACTATGA CGGAGAGGAC TCTGATTCTC CGCGGCATTC CACGGCTTCC 
AACAGTTCCA ACCTGAGCAG TCCTCCCAGC CCGGTGTCGC CCCGGAAGAC CAAGAGCCTG 
TCTCTGGAGA GCACAGACCG CGGGAGCTGG GACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062440.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062502.1 Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X15, mRNA.

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CloneID	OBu18517
Clone ID Related Accession (Same CDS sequence)	XM_006062440.2 , XM_006062440.1
Accession Version	XM_006062440.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006062440.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGCTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAGGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAATGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG CAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCGTA CAACGTCCAG TGGATGGCGA TCTTCAGTGA GCAGCTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAACGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CGGATAATAG CCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG CATTCAGTGG CTCGGTCAGT 
ATTCCGTCTA TCACCAAATC CCGCCCTGAA CCCGGTCGCT CCATGAGCGC CAGCAGTGGC 
CTATCTGCAA GGTCATCTGC ACAGAATGGC AGTGCGTTAA AGAGAGAATT CTCTGGAGGC 
AGCTACAGTG CAAAGCGGCA GCCCATGCCC TCCCCATCGG AGGGCTCGCT GTCCTCAGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCG CGGGACTATG ACGGAGAGGA CTCTGATTCT 
CCGCGGCATT CCACGGCTTC CAACAGTTCC AACCTGAGCA GTCCTCCCAG CCCGGTGTCG 
CCCCGGAAGA CCAAGAGCCT GTCTCTGGAG AGCACAGACC GCGGGAGCTG GGACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062502.1
CDS	766..5925
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062440.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062440.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGCTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAGGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAATGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG CAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCGTA CAACGTCCAG TGGATGGCGA TCTTCAGTGA GCAGCTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAACGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CGGATAATAG CCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG CATTCAGTGG CTCGGTCAGT 
ATTCCGTCTA TCACCAAATC CCGCCCTGAA CCCGGTCGCT CCATGAGCGC CAGCAGTGGC 
CTATCTGCAA GGTCATCTGC ACAGAATGGC AGTGCGTTAA AGAGAGAATT CTCTGGAGGC 
AGCTACAGTG CAAAGCGGCA GCCCATGCCC TCCCCATCGG AGGGCTCGCT GTCCTCAGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCG CGGGACTATG ACGGAGAGGA CTCTGATTCT 
CCGCGGCATT CCACGGCTTC CAACAGTTCC AACCTGAGCA GTCCTCCCAG CCCGGTGTCG 
CCCCGGAAGA CCAAGAGCCT GTCTCTGGAG AGCACAGACC GCGGGAGCTG GGACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062440.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062502.1 Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X15, mRNA.

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CloneID	OBu18517
Clone ID Related Accession (Same CDS sequence)	XM_006062440.2 , XM_006062440.1
Accession Version	XM_006062440.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGCTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAGGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAATGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG CAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCGTA CAACGTCCAG TGGATGGCGA TCTTCAGTGA GCAGCTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAACGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CGGATAATAG CCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG CATTCAGTGG CTCGGTCAGT 
ATTCCGTCTA TCACCAAATC CCGCCCTGAA CCCGGTCGCT CCATGAGCGC CAGCAGTGGC 
CTATCTGCAA GGTCATCTGC ACAGAATGGC AGTGCGTTAA AGAGAGAATT CTCTGGAGGC 
AGCTACAGTG CAAAGCGGCA GCCCATGCCC TCCCCATCGG AGGGCTCGCT GTCCTCAGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCG CGGGACTATG ACGGAGAGGA CTCTGATTCT 
CCGCGGCATT CCACGGCTTC CAACAGTTCC AACCTGAGCA GTCCTCCCAG CCCGGTGTCG 
CCCCGGAAGA CCAAGAGCCT GTCTCTGGAG AGCACAGACC GCGGGAGCTG GGACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062502.1
CDS	284..5443
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062440.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062440.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGCTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAGGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAATGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG CAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCGTA CAACGTCCAG TGGATGGCGA TCTTCAGTGA GCAGCTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAACGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CGGATAATAG CCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG CATTCAGTGG CTCGGTCAGT 
ATTCCGTCTA TCACCAAATC CCGCCCTGAA CCCGGTCGCT CCATGAGCGC CAGCAGTGGC 
CTATCTGCAA GGTCATCTGC ACAGAATGGC AGTGCGTTAA AGAGAGAATT CTCTGGAGGC 
AGCTACAGTG CAAAGCGGCA GCCCATGCCC TCCCCATCGG AGGGCTCGCT GTCCTCAGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCG CGGGACTATG ACGGAGAGGA CTCTGATTCT 
CCGCGGCATT CCACGGCTTC CAACAGTTCC AACCTGAGCA GTCCTCCCAG CCCGGTGTCG 
CCCCGGAAGA CCAAGAGCCT GTCTCTGGAG AGCACAGACC GCGGGAGCTG GGACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062441.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062503.1 Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X16, mRNA.

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CloneID	OBu18518
Clone ID Related Accession (Same CDS sequence)	XM_006062441.1
Accession Version	XM_006062441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGCTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAGGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAATGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG CAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCGTA CAACGTCCAG TGGATGGCGA TCTTCAGTGA GCAGCTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAACGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CGGATAATAG CCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG CATTCAGTGG CTCGGTCAGT 
ATTCCGTCTA TCACCAAATC CCGCCCTGAA CCCGGTCGCT CCATGAGCGC CAGCAGTGGC 
CTATCTGCAA GGTCATCTGC ACAGAATGGC AGTGCGTTAA AGAGAGAATT CTCTGGAGGC 
AGCTACAGTG CAAAGCGGCA GCCCATGCCC TCCCCATCGG AGGGCTCGCT GTCCTCAGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCG CGGGACTATG ACGGAGAGGA CTCTGATTCT 
CCGCGGCATT CCACGGCTTC CAACAGTTCC AACCTGAGCA GTCCTCCCAG CCCGGTGTCG 
CCCCGGAAGA CCAAGAGCCT GTCTCTGGAG AGCACAGACC GCGGGAGCTG GGACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062503.1
CDS	285..5384
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKASKGRRTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062441.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062441.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGCTA GCAAAGGCAG ACGTACTGTA GACTCTACTC CACTTCCAGT TCATACACCA 
ACCTTAAGGA AAAAGGGATG CCCTGGCTCA ACTGGCTTTC CATCGAAGCG CAAGATTCAT 
CAGTTTTTTG TCAAATCTTT TACTACTCCC ACCAGGTGTC ATCAGTGTAC ATCTTTGATG 
GTGGGTTTGA TAAGACAAGG CTGTTCTTGT GAAGTGTGTG GATTTTCATG TCACATAACT 
TGTGTAAACA AAGCTCCAAC TGCTTGTCCA GTTCCTCCTG AACAGACAAA AGGTCCTCTG 
GGTATAGATC CACAGAAAGG AATAGGAACA GCATATGAAG GGCATGTCAG GATCCCTAAG 
CCAGCTGGAG TGAAGAAAGG ATGGCAAAGA GCACTGGCTG TAGTCTGTGA CTTCAAGCTC 
TTTCTGTACG ATATTGCCGA AGGAAAGGCA TCTCAGCCCA GTGTTGTAGT CAGTCAAGTG 
ATTGACATGA GGGATGAAGA ATTTTCTGTG AGTTCAGTCT TGGCTTCTGA TGTTATTCAT 
GCAAGTCGAA AAGATATACC CTGTATATTT AGAGTCACAG CTTCCCAGCT CTCAGCATCT 
AGTAACAAAT GTTCCATCCT GATGCTAGCT GATAGTGAAA ATGAGAAGAG TAAATGGGTG 
GGAGTACTGA GTGAATTGCA CAAGATTTTG AAGAAAAACA AATTCAGAGA CCGCTCAGTC 
TATGTTCCCA AAGAGGCTTA TGACAGCACT CTGCCCCTCA TTAAAACAAC CCAGGCAGCT 
GCAATCATAG ATCATGAAAG AATAGCTTTG GGGAATGAAG AAGGGTTATT TGTTGTGCAT 
GTCACCAAAG ATGAAATTAT TAGAGTTGGT GACAATAAGA AGATTCATCA GATAGAACTC 
ATTCCAAATG ATCAACTTGT TGCCGTGATC TCAGGACGAA ATCGACACGT CCGACTTTTT 
CCTATGTCAG CTCTGGACGG GCGAGAGACT GATTTTTATA AGCTGGCAGA AACTAAAGGC 
TGTCAAACAA TAACTTCTGG CAAAGTGCGT CATGGAGCTC TTACATGCTT GTGTGTGGCT 
ATGAAAAGGC AGGTCCTCTG TTATGAACTA TTTCAGAGCA AGACGCGTCA CAGAAAATTT 
AAGGAAATTC AAGTCCCGTA CAACGTCCAG TGGATGGCGA TCTTCAGTGA GCAGCTCTGT 
GTGGGATTCC AGTCTGGATT TCTAAGATAT CCCCTGAATG GAGAAGGAAG TCCATACAGT 
ATGCTCCATT CAAATGACAA TACACTCTCA TTTATTGCAC ATCAACCAAT GGATGCCATC 
TGTGCGGTTG AAATCTCCAG TAAAGAATAC CTGCTGTGTT TTAACAGCAT TGGAATATAC 
ACTGACTGCC AGGGCCGAAG ATCTAGACAA CAGGAACTGA TGTGGCCAGC GAATCCTTCC 
TCTTGTTGTT ACAATGCACC GTATCTCTCA GTGTACAGTG AAAACGCAGT TGACATCTTT 
GATGTGAACT CCATGGAATG GATTCAGACT CTCCCACTCA AAAAGGTTCG CCCCTTAAAC 
AGTGAAGGAT CATTAAATCT TTTAGGCTTG GAGACGATTA GATTGATATA TTTCAAAAAT 
AAAATGGCAG AAGGGGATGA ACTGGTAGTT CCTGAAACAT CGGATAATAG CCGGAAGCAA 
ATGGTTAGAA ATATTAACAA TAAGAGGCGT TACTCTTTCA GAGTTCCGGA AGAGGAAAGA 
ATGCAACAGA GGAGGGAGAT GTTACGAGAT CCAGAAATGA GAAATAAATT AATTTCTAAC 
CCAACTAATT TTAACCACAT AGCACATATG GGTCCAGGAG ATGGAATACA GATCCTAAAA 
GACCTGCCCA TGAACCCTCG GCCTCAGGAA AGTCGGACAG CATTCAGTGG CTCGGTCAGT 
ATTCCGTCTA TCACCAAATC CCGCCCTGAA CCCGGTCGCT CCATGAGCGC CAGCAGTGGC 
CTATCTGCAA GGTCATCTGC ACAGAATGGC AGTGCGTTAA AGAGAGAATT CTCTGGAGGC 
AGCTACAGTG CAAAGCGGCA GCCCATGCCC TCCCCATCGG AGGGCTCGCT GTCCTCAGGA 
GGCATGGACC AGGGAAGTGA CGCCCCAGCG CGGGACTATG ACGGAGAGGA CTCTGATTCT 
CCGCGGCATT CCACGGCTTC CAACAGTTCC AACCTGAGCA GTCCTCCCAG CCCGGTGTCG 
CCCCGGAAGA CCAAGAGCCT GTCTCTGGAG AGCACAGACC GCGGGAGCTG GGACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062442.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062504.1 Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X17, mRNA.

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CloneID	OBu18519
Clone ID Related Accession (Same CDS sequence)	XM_006062442.1 , XM_006062442.2
Accession Version	XM_006062442.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785050.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGCTG GAAGTTCGTA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ACAGGAAATT GCGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAGAAT 
GAACTAGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGCGTATG CAGCATAGAA 
CATCAGCAAG AAATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTGT CCAAGAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTACATGATT CAGAAGGCCA ACAACTTGCT CTCAACAAAG AAATTATGAT TTTGAAAGAC 
AAATTGGAAA AAAACAGGAG AGAGAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAGCAACAGT ATGAACGAGA AAAAGTGTTA CTAACTGAAG AAAATAAAAA ACTGACAAGT 
GAGCTTGATA AGCTTACCAC TCTGTGTGAG AACTTAAGTG CACGCAACCA GCAGTTAGAA 
GAAGAGGTCA AAGATCTAGC AGATAAGAAG GAATCGGTTG CTCATTGGGA GGCTCAGATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT TCAAGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTAAGAAACT CCAGCTTGGG GACCCGAGCA 
ACAGATATGC CCTGGAAAAT GCGACGTTTT GCAAAACTGG ATATGTCAGC CAGACTGGAG 
TTACAGTCAG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGCTGAAT 
AAAGTTAAAG CATCTAACAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TATCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AACTGTAGAC TCTACTCCAC TTCCAGTTCA TACACCAACC 
TTAAGGAAAA AGGGATGCCC TGGCTCAACT GGCTTTCCAT CGAAGCGCAA GATTCATCAG 
TTTTTTGTCA AATCTTTTAC TACTCCCACC AGGTGTCATC AGTGTACATC TTTGATGGTG 
GGTTTGATAA GACAAGGCTG TTCTTGTGAA GTGTGTGGAT TTTCATGTCA CATAACTTGT 
GTAAACAAAG CTCCAACTGC TTGTCCAGTT CCTCCTGAAC AGACAAAAGG TCCTCTGGGT 
ATAGATCCAC AGAAAGGAAT AGGAACAGCA TATGAAGGGC ATGTCAGGAT CCCTAAGCCA 
GCTGGAGTGA AGAAAGGATG GCAAAGAGCA CTGGCTGTAG TCTGTGACTT CAAGCTCTTT 
CTGTACGATA TTGCCGAAGG AAAGGCATCT CAGCCCAGTG TTGTAGTCAG TCAAGTGATT 
GACATGAGGG ATGAAGAATT TTCTGTGAGT TCAGTCTTGG CTTCTGATGT TATTCATGCA 
AGTCGAAAAG ATATACCCTG TATATTTAGA GTCACAGCTT CCCAGCTCTC AGCATCTAGT 
AACAAATGTT CCATCCTGAT GCTAGCTGAT AGTGAAAATG AGAAGAGTAA ATGGGTGGGA 
GTACTGAGTG AATTGCACAA GATTTTGAAG AAAAACAAAT TCAGAGACCG CTCAGTCTAT 
GTTCCCAAAG AGGCTTATGA CAGCACTCTG CCCCTCATTA AAACAACCCA GGCAGCTGCA 
ATCATAGATC ATGAAAGAAT AGCTTTGGGG AATGAAGAAG GGTTATTTGT TGTGCATGTC 
ACCAAAGATG AAATTATTAG AGTTGGTGAC AATAAGAAGA TTCATCAGAT AGAACTCATT 
CCAAATGATC AACTTGTTGC CGTGATCTCA GGACGAAATC GACACGTCCG ACTTTTTCCT 
ATGTCAGCTC TGGACGGGCG AGAGACTGAT TTTTATAAGC TGGCAGAAAC TAAAGGCTGT 
CAAACAATAA CTTCTGGCAA AGTGCGTCAT GGAGCTCTTA CATGCTTGTG TGTGGCTATG 
AAAAGGCAGG TCCTCTGTTA TGAACTATTT CAGAGCAAGA CGCGTCACAG AAAATTTAAG 
GAAATTCAAG TCCCGTACAA CGTCCAGTGG ATGGCGATCT TCAGTGAGCA GCTCTGTGTG 
GGATTCCAGT CTGGATTTCT AAGATATCCC CTGAATGGAG AAGGAAGTCC ATACAGTATG 
CTCCATTCAA ATGACAATAC ACTCTCATTT ATTGCACATC AACCAATGGA TGCCATCTGT 
GCGGTTGAAA TCTCCAGTAA AGAATACCTG CTGTGTTTTA ACAGCATTGG AATATACACT 
GACTGCCAGG GCCGAAGATC TAGACAACAG GAACTGATGT GGCCAGCGAA TCCTTCCTCT 
TGTTGTTACA ATGCACCGTA TCTCTCAGTG TACAGTGAAA ACGCAGTTGA CATCTTTGAT 
GTGAACTCCA TGGAATGGAT TCAGACTCTC CCACTCAAAA AGGTTCGCCC CTTAAACAGT 
GAAGGATCAT TAAATCTTTT AGGCTTGGAG ACGATTAGAT TGATATATTT CAAAAATAAA 
ATGGCAGAAG GGGATGAACT GGTAGTTCCT GAAACATCGG ATAATAGCCG GAAGCAAATG 
GTTAGAAATA TTAACAATAA GAGGCGTTAC TCTTTCAGAG TTCCGGAAGA GGAAAGAATG 
CAACAGAGGA GGGAGATGTT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAACCCA 
ACTAATTTTA ACCACATAGC ACATATGGGT CCAGGAGATG GAATACAGAT CCTAAAAGAC 
CTGCCCATGA ACCCTCGGCC TCAGGAAAGT CGGACAGCAT TCAGTGGCTC GGTCAGTATT 
CCGTCTATCA CCAAATCCCG CCCTGAACCC GGTCGCTCCA TGAGCGCCAG CAGTGGCCTA 
TCTGCAAGGT CATCTGCACA GAATGGCAGT GCGTTAAAGA GAGAATTCTC TGGAGGCAGC 
TACAGTGCAA AGCGGCAGCC CATGCCCTCC CCATCGGAGG GCTCGCTGTC CTCAGGAGGC 
ATGGACCAGG GAAGTGACGC CCCAGCGCGG GACTATGACG GAGAGGACTC TGATTCTCCG 
CGGCATTCCA CGGCTTCCAA CAGTTCCAAC CTGAGCAGTC CTCCCAGCCC GGTGTCGCCC 
CGGAAGACCA AGAGCCTGTC TCTGGAGAGC ACAGACCGCG GGAGCTGGGA CCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062504.1
CDS	281..5197
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKLEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062442.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062442.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGCTG GAAGTTCGTA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ACAGGAAATT GCGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAGAAT 
GAACTAGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGCGTATG CAGCATAGAA 
CATCAGCAAG AAATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTGT CCAAGAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTACATGATT CAGAAGGCCA ACAACTTGCT CTCAACAAAG AAATTATGAT TTTGAAAGAC 
AAATTGGAAA AAAACAGGAG AGAGAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAGCAACAGT ATGAACGAGA AAAAGTGTTA CTAACTGAAG AAAATAAAAA ACTGACAAGT 
GAGCTTGATA AGCTTACCAC TCTGTGTGAG AACTTAAGTG CACGCAACCA GCAGTTAGAA 
GAAGAGGTCA AAGATCTAGC AGATAAGAAG GAATCGGTTG CTCATTGGGA GGCTCAGATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT TCAAGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTAAGAAACT CCAGCTTGGG GACCCGAGCA 
ACAGATATGC CCTGGAAAAT GCGACGTTTT GCAAAACTGG ATATGTCAGC CAGACTGGAG 
TTACAGTCAG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGCTGAAT 
AAAGTTAAAG CATCTAACAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TATCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AACTGTAGAC TCTACTCCAC TTCCAGTTCA TACACCAACC 
TTAAGGAAAA AGGGATGCCC TGGCTCAACT GGCTTTCCAT CGAAGCGCAA GATTCATCAG 
TTTTTTGTCA AATCTTTTAC TACTCCCACC AGGTGTCATC AGTGTACATC TTTGATGGTG 
GGTTTGATAA GACAAGGCTG TTCTTGTGAA GTGTGTGGAT TTTCATGTCA CATAACTTGT 
GTAAACAAAG CTCCAACTGC TTGTCCAGTT CCTCCTGAAC AGACAAAAGG TCCTCTGGGT 
ATAGATCCAC AGAAAGGAAT AGGAACAGCA TATGAAGGGC ATGTCAGGAT CCCTAAGCCA 
GCTGGAGTGA AGAAAGGATG GCAAAGAGCA CTGGCTGTAG TCTGTGACTT CAAGCTCTTT 
CTGTACGATA TTGCCGAAGG AAAGGCATCT CAGCCCAGTG TTGTAGTCAG TCAAGTGATT 
GACATGAGGG ATGAAGAATT TTCTGTGAGT TCAGTCTTGG CTTCTGATGT TATTCATGCA 
AGTCGAAAAG ATATACCCTG TATATTTAGA GTCACAGCTT CCCAGCTCTC AGCATCTAGT 
AACAAATGTT CCATCCTGAT GCTAGCTGAT AGTGAAAATG AGAAGAGTAA ATGGGTGGGA 
GTACTGAGTG AATTGCACAA GATTTTGAAG AAAAACAAAT TCAGAGACCG CTCAGTCTAT 
GTTCCCAAAG AGGCTTATGA CAGCACTCTG CCCCTCATTA AAACAACCCA GGCAGCTGCA 
ATCATAGATC ATGAAAGAAT AGCTTTGGGG AATGAAGAAG GGTTATTTGT TGTGCATGTC 
ACCAAAGATG AAATTATTAG AGTTGGTGAC AATAAGAAGA TTCATCAGAT AGAACTCATT 
CCAAATGATC AACTTGTTGC CGTGATCTCA GGACGAAATC GACACGTCCG ACTTTTTCCT 
ATGTCAGCTC TGGACGGGCG AGAGACTGAT TTTTATAAGC TGGCAGAAAC TAAAGGCTGT 
CAAACAATAA CTTCTGGCAA AGTGCGTCAT GGAGCTCTTA CATGCTTGTG TGTGGCTATG 
AAAAGGCAGG TCCTCTGTTA TGAACTATTT CAGAGCAAGA CGCGTCACAG AAAATTTAAG 
GAAATTCAAG TCCCGTACAA CGTCCAGTGG ATGGCGATCT TCAGTGAGCA GCTCTGTGTG 
GGATTCCAGT CTGGATTTCT AAGATATCCC CTGAATGGAG AAGGAAGTCC ATACAGTATG 
CTCCATTCAA ATGACAATAC ACTCTCATTT ATTGCACATC AACCAATGGA TGCCATCTGT 
GCGGTTGAAA TCTCCAGTAA AGAATACCTG CTGTGTTTTA ACAGCATTGG AATATACACT 
GACTGCCAGG GCCGAAGATC TAGACAACAG GAACTGATGT GGCCAGCGAA TCCTTCCTCT 
TGTTGTTACA ATGCACCGTA TCTCTCAGTG TACAGTGAAA ACGCAGTTGA CATCTTTGAT 
GTGAACTCCA TGGAATGGAT TCAGACTCTC CCACTCAAAA AGGTTCGCCC CTTAAACAGT 
GAAGGATCAT TAAATCTTTT AGGCTTGGAG ACGATTAGAT TGATATATTT CAAAAATAAA 
ATGGCAGAAG GGGATGAACT GGTAGTTCCT GAAACATCGG ATAATAGCCG GAAGCAAATG 
GTTAGAAATA TTAACAATAA GAGGCGTTAC TCTTTCAGAG TTCCGGAAGA GGAAAGAATG 
CAACAGAGGA GGGAGATGTT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAACCCA 
ACTAATTTTA ACCACATAGC ACATATGGGT CCAGGAGATG GAATACAGAT CCTAAAAGAC 
CTGCCCATGA ACCCTCGGCC TCAGGAAAGT CGGACAGCAT TCAGTGGCTC GGTCAGTATT 
CCGTCTATCA CCAAATCCCG CCCTGAACCC GGTCGCTCCA TGAGCGCCAG CAGTGGCCTA 
TCTGCAAGGT CATCTGCACA GAATGGCAGT GCGTTAAAGA GAGAATTCTC TGGAGGCAGC 
TACAGTGCAA AGCGGCAGCC CATGCCCTCC CCATCGGAGG GCTCGCTGTC CTCAGGAGGC 
ATGGACCAGG GAAGTGACGC CCCAGCGCGG GACTATGACG GAGAGGACTC TGATTCTCCG 
CGGCATTCCA CGGCTTCCAA CAGTTCCAAC CTGAGCAGTC CTCCCAGCCC GGTGTCGCCC 
CGGAAGACCA AGAGCCTGTC TCTGGAGAGC ACAGACCGCG GGAGCTGGGA CCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_006062442.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062504.1 Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X16, mRNA.

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CloneID	OBu18519
Clone ID Related Accession (Same CDS sequence)	XM_006062442.1 , XM_006062442.2
Accession Version	XM_006062442.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006062442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGCTG GAAGTTCGTA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ACAGGAAATT GCGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAGAAT 
GAACTAGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGCGTATG CAGCATAGAA 
CATCAGCAAG AAATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTGT CCAAGAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTACATGATT CAGAAGGCCA ACAACTTGCT CTCAACAAAG AAATTATGAT TTTGAAAGAC 
AAATTGGAAA AAAACAGGAG AGAGAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAGCAACAGT ATGAACGAGA AAAAGTGTTA CTAACTGAAG AAAATAAAAA ACTGACAAGT 
GAGCTTGATA AGCTTACCAC TCTGTGTGAG AACTTAAGTG CACGCAACCA GCAGTTAGAA 
GAAGAGGTCA AAGATCTAGC AGATAAGAAG GAATCGGTTG CTCATTGGGA GGCTCAGATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT TCAAGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTAAGAAACT CCAGCTTGGG GACCCGAGCA 
ACAGATATGC CCTGGAAAAT GCGACGTTTT GCAAAACTGG ATATGTCAGC CAGACTGGAG 
TTACAGTCAG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGCTGAAT 
AAAGTTAAAG CATCTAACAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TATCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AACTGTAGAC TCTACTCCAC TTCCAGTTCA TACACCAACC 
TTAAGGAAAA AGGGATGCCC TGGCTCAACT GGCTTTCCAT CGAAGCGCAA GATTCATCAG 
TTTTTTGTCA AATCTTTTAC TACTCCCACC AGGTGTCATC AGTGTACATC TTTGATGGTG 
GGTTTGATAA GACAAGGCTG TTCTTGTGAA GTGTGTGGAT TTTCATGTCA CATAACTTGT 
GTAAACAAAG CTCCAACTGC TTGTCCAGTT CCTCCTGAAC AGACAAAAGG TCCTCTGGGT 
ATAGATCCAC AGAAAGGAAT AGGAACAGCA TATGAAGGGC ATGTCAGGAT CCCTAAGCCA 
GCTGGAGTGA AGAAAGGATG GCAAAGAGCA CTGGCTGTAG TCTGTGACTT CAAGCTCTTT 
CTGTACGATA TTGCCGAAGG AAAGGCATCT CAGCCCAGTG TTGTAGTCAG TCAAGTGATT 
GACATGAGGG ATGAAGAATT TTCTGTGAGT TCAGTCTTGG CTTCTGATGT TATTCATGCA 
AGTCGAAAAG ATATACCCTG TATATTTAGA GTCACAGCTT CCCAGCTCTC AGCATCTAGT 
AACAAATGTT CCATCCTGAT GCTAGCTGAT AGTGAAAATG AGAAGAGTAA ATGGGTGGGA 
GTACTGAGTG AATTGCACAA GATTTTGAAG AAAAACAAAT TCAGAGACCG CTCAGTCTAT 
GTTCCCAAAG AGGCTTATGA CAGCACTCTG CCCCTCATTA AAACAACCCA GGCAGCTGCA 
ATCATAGATC ATGAAAGAAT AGCTTTGGGG AATGAAGAAG GGTTATTTGT TGTGCATGTC 
ACCAAAGATG AAATTATTAG AGTTGGTGAC AATAAGAAGA TTCATCAGAT AGAACTCATT 
CCAAATGATC AACTTGTTGC CGTGATCTCA GGACGAAATC GACACGTCCG ACTTTTTCCT 
ATGTCAGCTC TGGACGGGCG AGAGACTGAT TTTTATAAGC TGGCAGAAAC TAAAGGCTGT 
CAAACAATAA CTTCTGGCAA AGTGCGTCAT GGAGCTCTTA CATGCTTGTG TGTGGCTATG 
AAAAGGCAGG TCCTCTGTTA TGAACTATTT CAGAGCAAGA CGCGTCACAG AAAATTTAAG 
GAAATTCAAG TCCCGTACAA CGTCCAGTGG ATGGCGATCT TCAGTGAGCA GCTCTGTGTG 
GGATTCCAGT CTGGATTTCT AAGATATCCC CTGAATGGAG AAGGAAGTCC ATACAGTATG 
CTCCATTCAA ATGACAATAC ACTCTCATTT ATTGCACATC AACCAATGGA TGCCATCTGT 
GCGGTTGAAA TCTCCAGTAA AGAATACCTG CTGTGTTTTA ACAGCATTGG AATATACACT 
GACTGCCAGG GCCGAAGATC TAGACAACAG GAACTGATGT GGCCAGCGAA TCCTTCCTCT 
TGTTGTTACA ATGCACCGTA TCTCTCAGTG TACAGTGAAA ACGCAGTTGA CATCTTTGAT 
GTGAACTCCA TGGAATGGAT TCAGACTCTC CCACTCAAAA AGGTTCGCCC CTTAAACAGT 
GAAGGATCAT TAAATCTTTT AGGCTTGGAG ACGATTAGAT TGATATATTT CAAAAATAAA 
ATGGCAGAAG GGGATGAACT GGTAGTTCCT GAAACATCGG ATAATAGCCG GAAGCAAATG 
GTTAGAAATA TTAACAATAA GAGGCGTTAC TCTTTCAGAG TTCCGGAAGA GGAAAGAATG 
CAACAGAGGA GGGAGATGTT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAACCCA 
ACTAATTTTA ACCACATAGC ACATATGGGT CCAGGAGATG GAATACAGAT CCTAAAAGAC 
CTGCCCATGA ACCCTCGGCC TCAGGAAAGT CGGACAGCAT TCAGTGGCTC GGTCAGTATT 
CCGTCTATCA CCAAATCCCG CCCTGAACCC GGTCGCTCCA TGAGCGCCAG CAGTGGCCTA 
TCTGCAAGGT CATCTGCACA GAATGGCAGT GCGTTAAAGA GAGAATTCTC TGGAGGCAGC 
TACAGTGCAA AGCGGCAGCC CATGCCCTCC CCATCGGAGG GCTCGCTGTC CTCAGGAGGC 
ATGGACCAGG GAAGTGACGC CCCAGCGCGG GACTATGACG GAGAGGACTC TGATTCTCCG 
CGGCATTCCA CGGCTTCCAA CAGTTCCAAC CTGAGCAGTC CTCCCAGCCC GGTGTCGCCC 
CGGAAGACCA AGAGCCTGTC TCTGGAGAGC ACAGACCGCG GGAGCTGGGA CCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062504.1
CDS	766..5682
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKLEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_006062442.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062442.2

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGCTG GAAGTTCGTA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ACAGGAAATT GCGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAGAAT 
GAACTAGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGCGTATG CAGCATAGAA 
CATCAGCAAG AAATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTGT CCAAGAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTACATGATT CAGAAGGCCA ACAACTTGCT CTCAACAAAG AAATTATGAT TTTGAAAGAC 
AAATTGGAAA AAAACAGGAG AGAGAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAGCAACAGT ATGAACGAGA AAAAGTGTTA CTAACTGAAG AAAATAAAAA ACTGACAAGT 
GAGCTTGATA AGCTTACCAC TCTGTGTGAG AACTTAAGTG CACGCAACCA GCAGTTAGAA 
GAAGAGGTCA AAGATCTAGC AGATAAGAAG GAATCGGTTG CTCATTGGGA GGCTCAGATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT TCAAGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTAAGAAACT CCAGCTTGGG GACCCGAGCA 
ACAGATATGC CCTGGAAAAT GCGACGTTTT GCAAAACTGG ATATGTCAGC CAGACTGGAG 
TTACAGTCAG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGCTGAAT 
AAAGTTAAAG CATCTAACAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TATCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AACTGTAGAC TCTACTCCAC TTCCAGTTCA TACACCAACC 
TTAAGGAAAA AGGGATGCCC TGGCTCAACT GGCTTTCCAT CGAAGCGCAA GATTCATCAG 
TTTTTTGTCA AATCTTTTAC TACTCCCACC AGGTGTCATC AGTGTACATC TTTGATGGTG 
GGTTTGATAA GACAAGGCTG TTCTTGTGAA GTGTGTGGAT TTTCATGTCA CATAACTTGT 
GTAAACAAAG CTCCAACTGC TTGTCCAGTT CCTCCTGAAC AGACAAAAGG TCCTCTGGGT 
ATAGATCCAC AGAAAGGAAT AGGAACAGCA TATGAAGGGC ATGTCAGGAT CCCTAAGCCA 
GCTGGAGTGA AGAAAGGATG GCAAAGAGCA CTGGCTGTAG TCTGTGACTT CAAGCTCTTT 
CTGTACGATA TTGCCGAAGG AAAGGCATCT CAGCCCAGTG TTGTAGTCAG TCAAGTGATT 
GACATGAGGG ATGAAGAATT TTCTGTGAGT TCAGTCTTGG CTTCTGATGT TATTCATGCA 
AGTCGAAAAG ATATACCCTG TATATTTAGA GTCACAGCTT CCCAGCTCTC AGCATCTAGT 
AACAAATGTT CCATCCTGAT GCTAGCTGAT AGTGAAAATG AGAAGAGTAA ATGGGTGGGA 
GTACTGAGTG AATTGCACAA GATTTTGAAG AAAAACAAAT TCAGAGACCG CTCAGTCTAT 
GTTCCCAAAG AGGCTTATGA CAGCACTCTG CCCCTCATTA AAACAACCCA GGCAGCTGCA 
ATCATAGATC ATGAAAGAAT AGCTTTGGGG AATGAAGAAG GGTTATTTGT TGTGCATGTC 
ACCAAAGATG AAATTATTAG AGTTGGTGAC AATAAGAAGA TTCATCAGAT AGAACTCATT 
CCAAATGATC AACTTGTTGC CGTGATCTCA GGACGAAATC GACACGTCCG ACTTTTTCCT 
ATGTCAGCTC TGGACGGGCG AGAGACTGAT TTTTATAAGC TGGCAGAAAC TAAAGGCTGT 
CAAACAATAA CTTCTGGCAA AGTGCGTCAT GGAGCTCTTA CATGCTTGTG TGTGGCTATG 
AAAAGGCAGG TCCTCTGTTA TGAACTATTT CAGAGCAAGA CGCGTCACAG AAAATTTAAG 
GAAATTCAAG TCCCGTACAA CGTCCAGTGG ATGGCGATCT TCAGTGAGCA GCTCTGTGTG 
GGATTCCAGT CTGGATTTCT AAGATATCCC CTGAATGGAG AAGGAAGTCC ATACAGTATG 
CTCCATTCAA ATGACAATAC ACTCTCATTT ATTGCACATC AACCAATGGA TGCCATCTGT 
GCGGTTGAAA TCTCCAGTAA AGAATACCTG CTGTGTTTTA ACAGCATTGG AATATACACT 
GACTGCCAGG GCCGAAGATC TAGACAACAG GAACTGATGT GGCCAGCGAA TCCTTCCTCT 
TGTTGTTACA ATGCACCGTA TCTCTCAGTG TACAGTGAAA ACGCAGTTGA CATCTTTGAT 
GTGAACTCCA TGGAATGGAT TCAGACTCTC CCACTCAAAA AGGTTCGCCC CTTAAACAGT 
GAAGGATCAT TAAATCTTTT AGGCTTGGAG ACGATTAGAT TGATATATTT CAAAAATAAA 
ATGGCAGAAG GGGATGAACT GGTAGTTCCT GAAACATCGG ATAATAGCCG GAAGCAAATG 
GTTAGAAATA TTAACAATAA GAGGCGTTAC TCTTTCAGAG TTCCGGAAGA GGAAAGAATG 
CAACAGAGGA GGGAGATGTT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAACCCA 
ACTAATTTTA ACCACATAGC ACATATGGGT CCAGGAGATG GAATACAGAT CCTAAAAGAC 
CTGCCCATGA ACCCTCGGCC TCAGGAAAGT CGGACAGCAT TCAGTGGCTC GGTCAGTATT 
CCGTCTATCA CCAAATCCCG CCCTGAACCC GGTCGCTCCA TGAGCGCCAG CAGTGGCCTA 
TCTGCAAGGT CATCTGCACA GAATGGCAGT GCGTTAAAGA GAGAATTCTC TGGAGGCAGC 
TACAGTGCAA AGCGGCAGCC CATGCCCTCC CCATCGGAGG GCTCGCTGTC CTCAGGAGGC 
ATGGACCAGG GAAGTGACGC CCCAGCGCGG GACTATGACG GAGAGGACTC TGATTCTCCG 
CGGCATTCCA CGGCTTCCAA CAGTTCCAAC CTGAGCAGTC CTCCCAGCCC GGTGTCGCCC 
CGGAAGACCA AGAGCCTGTC TCTGGAGAGC ACAGACCGCG GGAGCTGGGA CCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_025286024.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141809.1 Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu66401
Clone ID Related Accession (Same CDS sequence)	XM_025286024.1
Accession Version	XM_025286024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AATCAAGTCA CTTGGAACAG CAACTTGAAG AAGCTAATTC TGTGAGGCGA 
GAATTAGATG ATGCTTTTAG ACAAATCAAG GCTTATGAAA AACAAATCAA GACATTACAA 
CAAGAAAGGG AAGAATTAAA TAAGGAACTA GTCCAGGCTA GTGAGCGATT AAAAAACCAA 
TCCAAAGAGC TGAAAGACGC ACACTGTCAG AGGAAACTGG CCATGCAGGA ATTCATGGAG 
ATCAATGAGC GGCTAACAGA ATTGCACACC CAAAAACAGA AACTTGCTCG CCATGTCCGA 
GATAAGGAAG AAGAGGTGGA CCTGGTGATG CAAAAAGTTG AAAGCTTAAG GCAAGAACTG 
CGCAGAACAG AAAGAGCCAA AAAAGAGCTG GAAGTTCGTA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ACAGGAAATT GCGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAGAAT 
GAACTAGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGCGTATG CAGCATAGAA 
CATCAGCAAG AAATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTGT CCAAGAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTACATGATT CAGAAGGCCA ACAACTTGCT CTCAACAAAG AAATTATGAT TTTGAAAGAC 
AAATTGGAAA AAAACAGGAG AGAGAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAGCAACAGT ATGAACGAGA AAAAGTGTTA CTAACTGAAG AAAATAAAAA ACTGACAAGT 
GAGCTTGATA AGCTTACCAC TCTGTGTGAG AACTTAAGTG CACGCAACCA GCAGTTAGAA 
GAAGAGGTCA AAGATCTAGC AGATAAGAAG GAATCGGTTG CTCATTGGGA GGCTCAGATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT TCAAGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTAAGAAACT CCAGCTTGGG GACCCGAGCA 
ACAGATATGC CCTGGAAAAT GCGACGTTTT GCAAAACTGG ATATGTCAGC CAGACTGGAG 
TTACAGTCAG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGCTGAAT 
AAAGTTAAAG CATCTAACAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TATCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AGATTCCTTT TCTTCTTCCT CATCTTCACT GATTGATTTT 
TTGGATGACC GCTCTCCATC CTGTACTCCA GCTAGCAAAG GCAGACGTAC TGATCCCGTT 
GAGAACACAT ATGTACGGAA TCCGAACGTC AAGTTTTACA TCCAGTCAAG GTCCACCTCT 
CCTTCCACAT CTAGTGAAGC TGAGCCAGTT AAGACTGTAG ACTCTACTCC ACTTCCAGTT 
CATACACCAA CCTTAAGGAA AAAGGGATGC CCTGGCTCAA CTGGCTTTCC ATCGAAGCGC 
AAGATTCATC AGTTTTTTGT CAAATCTTTT ACTACTCCCA CCAGGTGTCA TCAGTGTACA 
TCTTTGATGG TGGGTTTGAT AAGACAAGGC TGTTCTTGTG AAGTGTGTGG ATTTTCATGT 
CACATAACTT GTGTAAACAA AGCTCCAACT GCTTGTCCAG TTCCTCCTGA ACAGACAAAA 
GGTCCTCTGG GTATAGATCC ACAGAAAGGA ATAGGAACAG CATATGAAGG GCATGTCAGG 
ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA TGGCAAAGAG CACTGGCTGT AGTCTGTGAC 
TTCAAGCTCT TTCTGTACGA TATTGCCGAA GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC 
AGTCAAGTGA TTGACATGAG GGATGAAGAA TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT 
GTTATTCATG CAAGTCGAAA AGATATACCC TGTATATTTA GAGTCACAGC TTCCCAGCTC 
TCAGCATCTA GTAACAAATG TTCCATCCTG ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT 
AAATGGGTGG GAGTACTGAG TGAATTGCAC AAGATTTTGA AGAAAAACAA ATTCAGAGAC 
CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT GACAGCACTC TGCCCCTCAT TAAAACAACC 
CAGGCAGCTG CAATCATAGA TCATGAAAGA ATAGCTTTGG GGAATGAAGA AGGGTTATTT 
GTTGTGCATG TCACCAAAGA TGAAATTATT AGAGTTGGTG ACAATAAGAA GATTCATCAG 
ATAGAACTCA TTCCAAATGA TCAACTTGTT GCCGTGATCT CAGGACGAAA TCGACACGTC 
CGACTTTTTC CTATGTCAGC TCTGGACGGG CGAGAGACTG ATTTTTATAA GCTGGCAGAA 
ACTAAAGGCT GTCAAACAAT AACTTCTGGC AAAGTGCGTC ATGGAGCTCT TACATGCTTG 
TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT TATGAACTAT TTCAGAGCAA GACGCGTCAC 
AGAAAATTTA AGGAAATTCA AGTCCCGTAC AACGTCCAGT GGATGGCGAT CTTCAGTGAG 
CAGCTCTGTG TGGGATTCCA GTCTGGATTT CTAAGATATC CCCTGAATGG AGAAGGAAGT 
CCATACAGTA TGCTCCATTC AAATGACAAT ACACTCTCAT TTATTGCACA TCAACCAATG 
GATGCCATCT GTGCGGTTGA AATCTCCAGT AAAGAATACC TGCTGTGTTT TAACAGCATT 
GGAATATACA CTGACTGCCA GGGCCGAAGA TCTAGACAAC AGGAACTGAT GTGGCCAGCG 
AATCCTTCCT CTTGTTGTTA CAATGCACCG TATCTCTCAG TGTACAGTGA AAACGCAGTT 
GACATCTTTG ATGTGAACTC CATGGAATGG ATTCAGACTC TCCCACTCAA AAAGGTTCGC 
CCCTTAAACA GTGAAGGATC ATTAAATCTT TTAGGCTTGG AGACGATTAG ATTGATATAT 
TTCAAAAATA AAATGGCAGA AGGGGATGAA CTGGTAGTTC CTGAAACATC GGATAATAGC 
CGGAAGCAAA TGGTTAGAAA TATTAACAAT AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA 
GAGGAAAGAA TGCAACAGAG GAGGGAGATG TTACGAGATC CAGAAATGAG AAATAAATTA 
ATTTCTAACC CAACTAATTT TAACCACATA GCACATATGG GTCCAGGAGA TGGAATACAG 
ATCCTAAAAG ACCTGCCCAT GCCGGGTTTC CCTTATCCAT CCCCTCATCA TCACTCCGGT 
CTGATCTCCT CTCCGATCAA CTTTGAGCAC ATCTATCACA TGACTGTTAA TTCTGCTGAA 
AAATTTCTCT CTCCTGATTC CATAAACCCT GAATATTCCC CGTCTCTGCG CTCTGTCCCT 
GGTACACCAA GCTTTATGAC TCTGAGGAAC CCTCGGCCTC AGGAAAGTCG GACAGCATTC 
AGTGGCTCGG TCAGTATTCC GTCTATCACC AAATCCCGCC CTGAACCCGG TCGCTCCATG 
AGCGCCAGCA GTGGCCTATC TGCAAGGTCA TCTGCACAGA ATGGCAGTGC GTTAAAGAGA 
GAATTCTCTG GAGGCAGCTA CAGTGCAAAG CGGCAGCCCA TGCCCTCCCC ATCGGAGGGC 
TCGCTGTCCT CAGGAGGCAT GGACCAGGGA AGTGACGCCC CAGCGCGGGA CTATGACGGA 
GAGGACTCTG ATTCTCCGCG GCATTCCACG GCTTCCAACA GTTCCAACCT GAGCAGTCCT 
CCCAGCCCGG TGTCGCCCCG GAAGACCAAG AGCCTGTCTC TGGAGAGCAC AGACCGCGGG 
AGCTGGGACC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141809.1
CDS	766..6180
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_025286024.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025286024.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AATCAAGTCA CTTGGAACAG CAACTTGAAG AAGCTAATTC TGTGAGGCGA 
GAATTAGATG ATGCTTTTAG ACAAATCAAG GCTTATGAAA AACAAATCAA GACATTACAA 
CAAGAAAGGG AAGAATTAAA TAAGGAACTA GTCCAGGCTA GTGAGCGATT AAAAAACCAA 
TCCAAAGAGC TGAAAGACGC ACACTGTCAG AGGAAACTGG CCATGCAGGA ATTCATGGAG 
ATCAATGAGC GGCTAACAGA ATTGCACACC CAAAAACAGA AACTTGCTCG CCATGTCCGA 
GATAAGGAAG AAGAGGTGGA CCTGGTGATG CAAAAAGTTG AAAGCTTAAG GCAAGAACTG 
CGCAGAACAG AAAGAGCCAA AAAAGAGCTG GAAGTTCGTA CAGAAGCTGT GGCTGCTGAA 
GCATCTAAAG ACAGGAAATT GCGTGAACAG AGTGAGCACT ATTCTAAGCA ACTGGAGAAT 
GAACTAGAGG GACTGAAGCA AAAACAAATT AGTTACTCAC CAGGCGTATG CAGCATAGAA 
CATCAGCAAG AAATAACCAA ACTAAAGACT GATTTGGAAA AGAAAAGTAT CTTTTATGAA 
GAAGAATTGT CCAAGAGAGA AGGAATACAT GCAAATGAAA TTAAAAATCT GAAGAAAGAA 
CTACATGATT CAGAAGGCCA ACAACTTGCT CTCAACAAAG AAATTATGAT TTTGAAAGAC 
AAATTGGAAA AAAACAGGAG AGAGAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 
AAGCAACAGT ATGAACGAGA AAAAGTGTTA CTAACTGAAG AAAATAAAAA ACTGACAAGT 
GAGCTTGATA AGCTTACCAC TCTGTGTGAG AACTTAAGTG CACGCAACCA GCAGTTAGAA 
GAAGAGGTCA AAGATCTAGC AGATAAGAAG GAATCGGTTG CTCATTGGGA GGCTCAGATC 
ACAGAAATAA TTCAGTGGGT CAGTGATGAA AAGGATGCAC GAGGGTATCT TCAAGCCTTA 
GCTTCTAAAA TGACTGAAGA ATTGGAAGCT TTAAGAAACT CCAGCTTGGG GACCCGAGCA 
ACAGATATGC CCTGGAAAAT GCGACGTTTT GCAAAACTGG ATATGTCAGC CAGACTGGAG 
TTACAGTCAG CTCTGGATGC AGAAATAAGA GCCAAACAGG CCATCCAAGA AGAGCTGAAT 
AAAGTTAAAG CATCTAACAT CATAACAGAA TGTAAACTAA AAGATTCAGA GAAGAAGAAC 
TTGGAACTAC TATCAGAAAT CGAACAACTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 
AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 
GATGCTCTGG ATCAATTTGA AGATTCCTTT TCTTCTTCCT CATCTTCACT GATTGATTTT 
TTGGATGACC GCTCTCCATC CTGTACTCCA GCTAGCAAAG GCAGACGTAC TGATCCCGTT 
GAGAACACAT ATGTACGGAA TCCGAACGTC AAGTTTTACA TCCAGTCAAG GTCCACCTCT 
CCTTCCACAT CTAGTGAAGC TGAGCCAGTT AAGACTGTAG ACTCTACTCC ACTTCCAGTT 
CATACACCAA CCTTAAGGAA AAAGGGATGC CCTGGCTCAA CTGGCTTTCC ATCGAAGCGC 
AAGATTCATC AGTTTTTTGT CAAATCTTTT ACTACTCCCA CCAGGTGTCA TCAGTGTACA 
TCTTTGATGG TGGGTTTGAT AAGACAAGGC TGTTCTTGTG AAGTGTGTGG ATTTTCATGT 
CACATAACTT GTGTAAACAA AGCTCCAACT GCTTGTCCAG TTCCTCCTGA ACAGACAAAA 
GGTCCTCTGG GTATAGATCC ACAGAAAGGA ATAGGAACAG CATATGAAGG GCATGTCAGG 
ATCCCTAAGC CAGCTGGAGT GAAGAAAGGA TGGCAAAGAG CACTGGCTGT AGTCTGTGAC 
TTCAAGCTCT TTCTGTACGA TATTGCCGAA GGAAAGGCAT CTCAGCCCAG TGTTGTAGTC 
AGTCAAGTGA TTGACATGAG GGATGAAGAA TTTTCTGTGA GTTCAGTCTT GGCTTCTGAT 
GTTATTCATG CAAGTCGAAA AGATATACCC TGTATATTTA GAGTCACAGC TTCCCAGCTC 
TCAGCATCTA GTAACAAATG TTCCATCCTG ATGCTAGCTG ATAGTGAAAA TGAGAAGAGT 
AAATGGGTGG GAGTACTGAG TGAATTGCAC AAGATTTTGA AGAAAAACAA ATTCAGAGAC 
CGCTCAGTCT ATGTTCCCAA AGAGGCTTAT GACAGCACTC TGCCCCTCAT TAAAACAACC 
CAGGCAGCTG CAATCATAGA TCATGAAAGA ATAGCTTTGG GGAATGAAGA AGGGTTATTT 
GTTGTGCATG TCACCAAAGA TGAAATTATT AGAGTTGGTG ACAATAAGAA GATTCATCAG 
ATAGAACTCA TTCCAAATGA TCAACTTGTT GCCGTGATCT CAGGACGAAA TCGACACGTC 
CGACTTTTTC CTATGTCAGC TCTGGACGGG CGAGAGACTG ATTTTTATAA GCTGGCAGAA 
ACTAAAGGCT GTCAAACAAT AACTTCTGGC AAAGTGCGTC ATGGAGCTCT TACATGCTTG 
TGTGTGGCTA TGAAAAGGCA GGTCCTCTGT TATGAACTAT TTCAGAGCAA GACGCGTCAC 
AGAAAATTTA AGGAAATTCA AGTCCCGTAC AACGTCCAGT GGATGGCGAT CTTCAGTGAG 
CAGCTCTGTG TGGGATTCCA GTCTGGATTT CTAAGATATC CCCTGAATGG AGAAGGAAGT 
CCATACAGTA TGCTCCATTC AAATGACAAT ACACTCTCAT TTATTGCACA TCAACCAATG 
GATGCCATCT GTGCGGTTGA AATCTCCAGT AAAGAATACC TGCTGTGTTT TAACAGCATT 
GGAATATACA CTGACTGCCA GGGCCGAAGA TCTAGACAAC AGGAACTGAT GTGGCCAGCG 
AATCCTTCCT CTTGTTGTTA CAATGCACCG TATCTCTCAG TGTACAGTGA AAACGCAGTT 
GACATCTTTG ATGTGAACTC CATGGAATGG ATTCAGACTC TCCCACTCAA AAAGGTTCGC 
CCCTTAAACA GTGAAGGATC ATTAAATCTT TTAGGCTTGG AGACGATTAG ATTGATATAT 
TTCAAAAATA AAATGGCAGA AGGGGATGAA CTGGTAGTTC CTGAAACATC GGATAATAGC 
CGGAAGCAAA TGGTTAGAAA TATTAACAAT AAGAGGCGTT ACTCTTTCAG AGTTCCGGAA 
GAGGAAAGAA TGCAACAGAG GAGGGAGATG TTACGAGATC CAGAAATGAG AAATAAATTA 
ATTTCTAACC CAACTAATTT TAACCACATA GCACATATGG GTCCAGGAGA TGGAATACAG 
ATCCTAAAAG ACCTGCCCAT GCCGGGTTTC CCTTATCCAT CCCCTCATCA TCACTCCGGT 
CTGATCTCCT CTCCGATCAA CTTTGAGCAC ATCTATCACA TGACTGTTAA TTCTGCTGAA 
AAATTTCTCT CTCCTGATTC CATAAACCCT GAATATTCCC CGTCTCTGCG CTCTGTCCCT 
GGTACACCAA GCTTTATGAC TCTGAGGAAC CCTCGGCCTC AGGAAAGTCG GACAGCATTC 
AGTGGCTCGG TCAGTATTCC GTCTATCACC AAATCCCGCC CTGAACCCGG TCGCTCCATG 
AGCGCCAGCA GTGGCCTATC TGCAAGGTCA TCTGCACAGA ATGGCAGTGC GTTAAAGAGA 
GAATTCTCTG GAGGCAGCTA CAGTGCAAAG CGGCAGCCCA TGCCCTCCCC ATCGGAGGGC 
TCGCTGTCCT CAGGAGGCAT GGACCAGGGA AGTGACGCCC CAGCGCGGGA CTATGACGGA 
GAGGACTCTG ATTCTCCGCG GCATTCCACG GCTTCCAACA GTTCCAACCT GAGCAGTCCT 
CCCAGCCCGG TGTCGCCCCG GAAGACCAAG AGCCTGTCTC TGGAGAGCAC AGACCGCGGG 
AGCTGGGACC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_025286025.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141810.1 Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X10, mRNA.

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CloneID	OBu66402
Clone ID Related Accession (Same CDS sequence)	XM_025286025.1
Accession Version	XM_025286025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATTAC ATAGAGAAGA CTTTGAAATA TTAAAGGTGA TTGGTCGAGG GGCTTTTGGA 
GAGGTTGCTG TAGTGAAACT AAAAAATGCA GATAAAGTAT TTGCCATGAA AATATTGAAT 
AAATGGGAAA TGCTGAAAAG AGCTGAGACA GCATGTTTTC GTGAAGAAAG GGATGTATTA 
GTGAATGGAG ACAATAAATG GATTACAACT TTGCATTATG CTTTCCAGGA TGACAATAAC 
TTATACCTGG TTATGGATTA TTATGTTGGT GGGGATTTGC TTACTCTACT CAGCAAATTT 
GAAGATAGAT TACCTGAAGA CATGGCTCGA TTTTACTTGG CTGAGATGGT GATAGCAATT 
GATTCAGTTC ATCAGCTGCA TTATGTACAC AGAGACATCA AACCTGACAA TATATTGATG 
GATATGAATG GACATATTCG GTTAGCAGAT TTTGGTTCAT GTCTGAAGCT GATGGAAGAT 
GGAACGGTCC AATCCTCAGT GGCTGTTGGA ACTCCAGATT ATATCTCTCC TGAAATCCTT 
CAAGCCATGG AAGATGGAAA AGGCAGATAT GGACCTGAGT GTGACTGGTG GTCTTTGGGG 
GTCTGTATGT ATGAAATGCT TTATGGAGAA ACACCATTTT ATGCAGAATC TCTGGTGGAA 
ACATATGGAA AAATCATGAA TCACAAAGAG AGGTTTCAGT TTCCTCCTCA AGTGAGTGAT 
GTGTCTGAAA ATGCTAAGGA TCTTATTCGA AGACTCATCT GTAGCAGAGA ACATCGACTT 
GGTCAAAATG GAATAGAAGA CTTTAAGAAG CACCCATTTT TCAATGGAAT TGATTGGGAT 
AATATTCGAA ACTGTGAAGC ACCTTATATT CCAGAAGTTA GTAGCCCAAC AGATACGTCG 
AATTTTGATG TGGATGATGA TTGTTTAAAG AATTCTGAAA CGATGCCCCC ACCAACACAT 
ACTGCCTTTT CTGGTCACCA TCTGCCATTT GTTGGTTTTA CATATACCAG TAGCTGTGTT 
CTTTCTGATC GAAGCTGTTT AAGAGTTACA GCTGGTCCCA CACCATTGGA TCTCGATGTT 
AATGTGCAGA GGACTCTAGA TAACAACTTA GCAACTGAAG CTTATGAAAG AAGAATTAAG 
CGCCTTGAGC AGGAAAAACT TGAACTTAGT AGAAAGCTTC AAGAGTCAAC GCAGACTGTC 
CAGGCTCTGC AGTATTCAAC TGCTGATGGT CCACTAACAG CAAGCAAAGA TTTAGAAATA 
AAAAGCTTAA AAGAAGAAAT TGAAAAACTA AGGAAACAAG TAAGAGAATC AAGTCACTTG 
GAACAGCAAC TTGAAGAAGC TAATTCTGTG AGGCGAGAAT TAGATGATGC TTTTAGACAA 
ATCAAGGCTT ATGAAAAACA AATCAAGACA TTACAACAAG AAAGGGAAGA ATTAAATAAG 
GAACTAGTCC AGGCTAGTGA GCGATTAAAA AACCAATCCA AAGAGCTGAA AGACGCACAC 
TGTCAGAGGA AACTGGCCAT GCAGGAATTC ATGGAGATCA ATGAGCGGCT AACAGAATTG 
CACACCCAAA AACAGAAACT TGCTCGCCAT GTCCGAGATA AGGAAGAAGA GGTGGACCTG 
GTGATGCAAA AAGTTGAAAG CTTAAGGCAA GAACTGCGCA GAACAGAAAG AGCCAAAAAA 
GAGCTGGAAG TTCGTACAGA AGCTGTGGCT GCTGAAGCAT CTAAAGACAG GAAATTGCGT 
GAACAGAGTG AGCACTATTC TAAGCAACTG GAGAATGAAC TAGAGGGACT GAAGCAAAAA 
CAAATTAGTT ACTCACCAGG CGTATGCAGC ATAGAACATC AGCAAGAAAT AACCAAACTA 
AAGACTGATT TGGAAAAGAA AAGTATCTTT TATGAAGAAG AATTGTCCAA GAGAGAAGGA 
ATACATGCAA ATGAAATTAA AAATCTGAAG AAAGAACTAC ATGATTCAGA AGGCCAACAA 
CTTGCTCTCA ACAAAGAAAT TATGATTTTG AAAGACAAAT TGGAAAAAAA CAGGAGAGAG 
AGTCAAAGTG AAAGGGAAGA ATTTGAAAAT GAGTTCAAGC AACAGTATGA ACGAGAAAAA 
GTGTTACTAA CTGAAGAAAA TAAAAAACTG ACAAGTGAGC TTGATAAGCT TACCACTCTG 
TGTGAGAACT TAAGTGCACG CAACCAGCAG TTAGAAGAAG AGGTCAAAGA TCTAGCAGAT 
AAGAAGGAAT CGGTTGCTCA TTGGGAGGCT CAGATCACAG AAATAATTCA GTGGGTCAGT 
GATGAAAAGG ATGCACGAGG GTATCTTCAA GCCTTAGCTT CTAAAATGAC TGAAGAATTG 
GAAGCTTTAA GAAACTCCAG CTTGGGGACC CGAGCAACAG ATATGCCCTG GAAAATGCGA 
CGTTTTGCAA AACTGGATAT GTCAGCCAGA CTGGAGTTAC AGTCAGCTCT GGATGCAGAA 
ATAAGAGCCA AACAGGCCAT CCAAGAAGAG CTGAATAAAG TTAAAGCATC TAACATCATA 
ACAGAATGTA AACTAAAAGA TTCAGAGAAG AAGAACTTGG AACTACTATC AGAAATCGAA 
CAACTGATAA AGGACACTGA AGAGCTTAGA TCTGAAAAGG GTATAGAGCA CCAAGACTCA 
CAGCATTCTT TCTTGGCATT TTTGAATACG CCTACCGATG CTCTGGATCA ATTTGAAGAT 
TCCTTTTCTT CTTCCTCATC TTCACTGATT GATTTTTTGG ATGACCGCTC TCCATCCTGT 
ACTCCAGCTA GCAAAGGCAG ACGTACTGAT CCCGTTGAGA ACACATATGT ACGGAATCCG 
AACGTCAAGT TTTACATCCA GTCAAGGTCC ACCTCTCCTT CCACATCTAG TGAAGCTGAG 
CCAGTTAAGA CTGTAGACTC TACTCCACTT CCAGTTCATA CACCAACCTT AAGGAAAAAG 
GGATGCCCTG GCTCAACTGG CTTTCCATCG AAGCGCAAGA TTCATCAGTT TTTTGTCAAA 
TCTTTTACTA CTCCCACCAG GTGTCATCAG TGTACATCTT TGATGGTGGG TTTGATAAGA 
CAAGGCTGTT CTTGTGAAGT GTGTGGATTT TCATGTCACA TAACTTGTGT AAACAAAGCT 
CCAACTGCTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CTCTGGGTAT AGATCCACAG 
AAAGGAATAG GAACAGCATA TGAAGGGCAT GTCAGGATCC CTAAGCCAGC TGGAGTGAAG 
AAAGGATGGC AAAGAGCACT GGCTGTAGTC TGTGACTTCA AGCTCTTTCT GTACGATATT 
GCCGAAGGAA AGGCATCTCA GCCCAGTGTT GTAGTCAGTC AAGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TTCATGCAAG TCGAAAAGAT 
ATACCCTGTA TATTTAGAGT CACAGCTTCC CAGCTCTCAG CATCTAGTAA CAAATGTTCC 
ATCCTGATGC TAGCTGATAG TGAAAATGAG AAGAGTAAAT GGGTGGGAGT ACTGAGTGAA 
TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG 
GCTTATGACA GCACTCTGCC CCTCATTAAA ACAACCCAGG CAGCTGCAAT CATAGATCAT 
GAAAGAATAG CTTTGGGGAA TGAAGAAGGG TTATTTGTTG TGCATGTCAC CAAAGATGAA 
ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATAG AACTCATTCC AAATGATCAA 
CTTGTTGCCG TGATCTCAGG ACGAAATCGA CACGTCCGAC TTTTTCCTAT GTCAGCTCTG 
GACGGGCGAG AGACTGATTT TTATAAGCTG GCAGAAACTA AAGGCTGTCA AACAATAACT 
TCTGGCAAAG TGCGTCATGG AGCTCTTACA TGCTTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACG CGTCACAGAA AATTTAAGGA AATTCAAGTC 
CCGTACAACG TCCAGTGGAT GGCGATCTTC AGTGAGCAGC TCTGTGTGGG ATTCCAGTCT 
GGATTTCTAA GATATCCCCT GAATGGAGAA GGAAGTCCAT ACAGTATGCT CCATTCAAAT 
GACAATACAC TCTCATTTAT TGCACATCAA CCAATGGATG CCATCTGTGC GGTTGAAATC 
TCCAGTAAAG AATACCTGCT GTGTTTTAAC AGCATTGGAA TATACACTGA CTGCCAGGGC 
CGAAGATCTA GACAACAGGA ACTGATGTGG CCAGCGAATC CTTCCTCTTG TTGTTACAAT 
GCACCGTATC TCTCAGTGTA CAGTGAAAAC GCAGTTGACA TCTTTGATGT GAACTCCATG 
GAATGGATTC AGACTCTCCC ACTCAAAAAG GTTCGCCCCT TAAACAGTGA AGGATCATTA 
AATCTTTTAG GCTTGGAGAC GATTAGATTG ATATATTTCA AAAATAAAAT GGCAGAAGGG 
GATGAACTGG TAGTTCCTGA AACATCGGAT AATAGCCGGA AGCAAATGGT TAGAAATATT 
AACAATAAGA GGCGTTACTC TTTCAGAGTT CCGGAAGAGG AAAGAATGCA ACAGAGGAGG 
GAGATGTTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAACCCAAC TAATTTTAAC 
CACATAGCAC ATATGGGTCC AGGAGATGGA ATACAGATCC TAAAAGACCT GCCCATGCCG 
GGTTTCCCTT ATCCATCCCC TCATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT 
GAGCACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA 
AACCCTGAAT ATTCCCCGTC TCTGCGCTCT GTCCCTGGTA CACCAAGCTT TATGACTCTG 
AGGAACCCTC GGCCTCAGGA AAGTCGGACA GCATTCAGTG GCTCGGTCAG TATTCCGTCT 
ATCACCAAAT CCCGCCCTGA ACCCGGTCGC TCCATGAGCG CCAGCAGTGG CCTATCTGCA 
AGGTCATCTG CACAGAATGG CAGTGCGTTA AAGAGAGAAT TCTCTGGAGG CAGCTACAGT 
GCAAAGCGGC AGCCCATGCC CTCCCCATCG GAGGGCTCGC TGTCCTCAGG AGGCATGGAC 
CAGGGAAGTG ACGCCCCAGC GCGGGACTAT GACGGAGAGG ACTCTGATTC TCCGCGGCAT 
TCCACGGCTT CCAACAGTTC CAACCTGAGC AGTCCTCCCA GCCCGGTGTC GCCCCGGAAG 
ACCAAGAGCC TGTCTCTGGA GAGCACAGAC CGCGGGAGCT GGGACCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141810.1
CDS	390..5720
Translation	MRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLRNPRPQESRTAFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP

Target ORF information:

RefSeq Version	XM_025286025.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025286025.1

ORF Insert Sequence:

ATGCGATTAC ATAGAGAAGA CTTTGAAATA TTAAAGGTGA TTGGTCGAGG GGCTTTTGGA 
GAGGTTGCTG TAGTGAAACT AAAAAATGCA GATAAAGTAT TTGCCATGAA AATATTGAAT 
AAATGGGAAA TGCTGAAAAG AGCTGAGACA GCATGTTTTC GTGAAGAAAG GGATGTATTA 
GTGAATGGAG ACAATAAATG GATTACAACT TTGCATTATG CTTTCCAGGA TGACAATAAC 
TTATACCTGG TTATGGATTA TTATGTTGGT GGGGATTTGC TTACTCTACT CAGCAAATTT 
GAAGATAGAT TACCTGAAGA CATGGCTCGA TTTTACTTGG CTGAGATGGT GATAGCAATT 
GATTCAGTTC ATCAGCTGCA TTATGTACAC AGAGACATCA AACCTGACAA TATATTGATG 
GATATGAATG GACATATTCG GTTAGCAGAT TTTGGTTCAT GTCTGAAGCT GATGGAAGAT 
GGAACGGTCC AATCCTCAGT GGCTGTTGGA ACTCCAGATT ATATCTCTCC TGAAATCCTT 
CAAGCCATGG AAGATGGAAA AGGCAGATAT GGACCTGAGT GTGACTGGTG GTCTTTGGGG 
GTCTGTATGT ATGAAATGCT TTATGGAGAA ACACCATTTT ATGCAGAATC TCTGGTGGAA 
ACATATGGAA AAATCATGAA TCACAAAGAG AGGTTTCAGT TTCCTCCTCA AGTGAGTGAT 
GTGTCTGAAA ATGCTAAGGA TCTTATTCGA AGACTCATCT GTAGCAGAGA ACATCGACTT 
GGTCAAAATG GAATAGAAGA CTTTAAGAAG CACCCATTTT TCAATGGAAT TGATTGGGAT 
AATATTCGAA ACTGTGAAGC ACCTTATATT CCAGAAGTTA GTAGCCCAAC AGATACGTCG 
AATTTTGATG TGGATGATGA TTGTTTAAAG AATTCTGAAA CGATGCCCCC ACCAACACAT 
ACTGCCTTTT CTGGTCACCA TCTGCCATTT GTTGGTTTTA CATATACCAG TAGCTGTGTT 
CTTTCTGATC GAAGCTGTTT AAGAGTTACA GCTGGTCCCA CACCATTGGA TCTCGATGTT 
AATGTGCAGA GGACTCTAGA TAACAACTTA GCAACTGAAG CTTATGAAAG AAGAATTAAG 
CGCCTTGAGC AGGAAAAACT TGAACTTAGT AGAAAGCTTC AAGAGTCAAC GCAGACTGTC 
CAGGCTCTGC AGTATTCAAC TGCTGATGGT CCACTAACAG CAAGCAAAGA TTTAGAAATA 
AAAAGCTTAA AAGAAGAAAT TGAAAAACTA AGGAAACAAG TAAGAGAATC AAGTCACTTG 
GAACAGCAAC TTGAAGAAGC TAATTCTGTG AGGCGAGAAT TAGATGATGC TTTTAGACAA 
ATCAAGGCTT ATGAAAAACA AATCAAGACA TTACAACAAG AAAGGGAAGA ATTAAATAAG 
GAACTAGTCC AGGCTAGTGA GCGATTAAAA AACCAATCCA AAGAGCTGAA AGACGCACAC 
TGTCAGAGGA AACTGGCCAT GCAGGAATTC ATGGAGATCA ATGAGCGGCT AACAGAATTG 
CACACCCAAA AACAGAAACT TGCTCGCCAT GTCCGAGATA AGGAAGAAGA GGTGGACCTG 
GTGATGCAAA AAGTTGAAAG CTTAAGGCAA GAACTGCGCA GAACAGAAAG AGCCAAAAAA 
GAGCTGGAAG TTCGTACAGA AGCTGTGGCT GCTGAAGCAT CTAAAGACAG GAAATTGCGT 
GAACAGAGTG AGCACTATTC TAAGCAACTG GAGAATGAAC TAGAGGGACT GAAGCAAAAA 
CAAATTAGTT ACTCACCAGG CGTATGCAGC ATAGAACATC AGCAAGAAAT AACCAAACTA 
AAGACTGATT TGGAAAAGAA AAGTATCTTT TATGAAGAAG AATTGTCCAA GAGAGAAGGA 
ATACATGCAA ATGAAATTAA AAATCTGAAG AAAGAACTAC ATGATTCAGA AGGCCAACAA 
CTTGCTCTCA ACAAAGAAAT TATGATTTTG AAAGACAAAT TGGAAAAAAA CAGGAGAGAG 
AGTCAAAGTG AAAGGGAAGA ATTTGAAAAT GAGTTCAAGC AACAGTATGA ACGAGAAAAA 
GTGTTACTAA CTGAAGAAAA TAAAAAACTG ACAAGTGAGC TTGATAAGCT TACCACTCTG 
TGTGAGAACT TAAGTGCACG CAACCAGCAG TTAGAAGAAG AGGTCAAAGA TCTAGCAGAT 
AAGAAGGAAT CGGTTGCTCA TTGGGAGGCT CAGATCACAG AAATAATTCA GTGGGTCAGT 
GATGAAAAGG ATGCACGAGG GTATCTTCAA GCCTTAGCTT CTAAAATGAC TGAAGAATTG 
GAAGCTTTAA GAAACTCCAG CTTGGGGACC CGAGCAACAG ATATGCCCTG GAAAATGCGA 
CGTTTTGCAA AACTGGATAT GTCAGCCAGA CTGGAGTTAC AGTCAGCTCT GGATGCAGAA 
ATAAGAGCCA AACAGGCCAT CCAAGAAGAG CTGAATAAAG TTAAAGCATC TAACATCATA 
ACAGAATGTA AACTAAAAGA TTCAGAGAAG AAGAACTTGG AACTACTATC AGAAATCGAA 
CAACTGATAA AGGACACTGA AGAGCTTAGA TCTGAAAAGG GTATAGAGCA CCAAGACTCA 
CAGCATTCTT TCTTGGCATT TTTGAATACG CCTACCGATG CTCTGGATCA ATTTGAAGAT 
TCCTTTTCTT CTTCCTCATC TTCACTGATT GATTTTTTGG ATGACCGCTC TCCATCCTGT 
ACTCCAGCTA GCAAAGGCAG ACGTACTGAT CCCGTTGAGA ACACATATGT ACGGAATCCG 
AACGTCAAGT TTTACATCCA GTCAAGGTCC ACCTCTCCTT CCACATCTAG TGAAGCTGAG 
CCAGTTAAGA CTGTAGACTC TACTCCACTT CCAGTTCATA CACCAACCTT AAGGAAAAAG 
GGATGCCCTG GCTCAACTGG CTTTCCATCG AAGCGCAAGA TTCATCAGTT TTTTGTCAAA 
TCTTTTACTA CTCCCACCAG GTGTCATCAG TGTACATCTT TGATGGTGGG TTTGATAAGA 
CAAGGCTGTT CTTGTGAAGT GTGTGGATTT TCATGTCACA TAACTTGTGT AAACAAAGCT 
CCAACTGCTT GTCCAGTTCC TCCTGAACAG ACAAAAGGTC CTCTGGGTAT AGATCCACAG 
AAAGGAATAG GAACAGCATA TGAAGGGCAT GTCAGGATCC CTAAGCCAGC TGGAGTGAAG 
AAAGGATGGC AAAGAGCACT GGCTGTAGTC TGTGACTTCA AGCTCTTTCT GTACGATATT 
GCCGAAGGAA AGGCATCTCA GCCCAGTGTT GTAGTCAGTC AAGTGATTGA CATGAGGGAT 
GAAGAATTTT CTGTGAGTTC AGTCTTGGCT TCTGATGTTA TTCATGCAAG TCGAAAAGAT 
ATACCCTGTA TATTTAGAGT CACAGCTTCC CAGCTCTCAG CATCTAGTAA CAAATGTTCC 
ATCCTGATGC TAGCTGATAG TGAAAATGAG AAGAGTAAAT GGGTGGGAGT ACTGAGTGAA 
TTGCACAAGA TTTTGAAGAA AAACAAATTC AGAGACCGCT CAGTCTATGT TCCCAAAGAG 
GCTTATGACA GCACTCTGCC CCTCATTAAA ACAACCCAGG CAGCTGCAAT CATAGATCAT 
GAAAGAATAG CTTTGGGGAA TGAAGAAGGG TTATTTGTTG TGCATGTCAC CAAAGATGAA 
ATTATTAGAG TTGGTGACAA TAAGAAGATT CATCAGATAG AACTCATTCC AAATGATCAA 
CTTGTTGCCG TGATCTCAGG ACGAAATCGA CACGTCCGAC TTTTTCCTAT GTCAGCTCTG 
GACGGGCGAG AGACTGATTT TTATAAGCTG GCAGAAACTA AAGGCTGTCA AACAATAACT 
TCTGGCAAAG TGCGTCATGG AGCTCTTACA TGCTTGTGTG TGGCTATGAA AAGGCAGGTC 
CTCTGTTATG AACTATTTCA GAGCAAGACG CGTCACAGAA AATTTAAGGA AATTCAAGTC 
CCGTACAACG TCCAGTGGAT GGCGATCTTC AGTGAGCAGC TCTGTGTGGG ATTCCAGTCT 
GGATTTCTAA GATATCCCCT GAATGGAGAA GGAAGTCCAT ACAGTATGCT CCATTCAAAT 
GACAATACAC TCTCATTTAT TGCACATCAA CCAATGGATG CCATCTGTGC GGTTGAAATC 
TCCAGTAAAG AATACCTGCT GTGTTTTAAC AGCATTGGAA TATACACTGA CTGCCAGGGC 
CGAAGATCTA GACAACAGGA ACTGATGTGG CCAGCGAATC CTTCCTCTTG TTGTTACAAT 
GCACCGTATC TCTCAGTGTA CAGTGAAAAC GCAGTTGACA TCTTTGATGT GAACTCCATG 
GAATGGATTC AGACTCTCCC ACTCAAAAAG GTTCGCCCCT TAAACAGTGA AGGATCATTA 
AATCTTTTAG GCTTGGAGAC GATTAGATTG ATATATTTCA AAAATAAAAT GGCAGAAGGG 
GATGAACTGG TAGTTCCTGA AACATCGGAT AATAGCCGGA AGCAAATGGT TAGAAATATT 
AACAATAAGA GGCGTTACTC TTTCAGAGTT CCGGAAGAGG AAAGAATGCA ACAGAGGAGG 
GAGATGTTAC GAGATCCAGA AATGAGAAAT AAATTAATTT CTAACCCAAC TAATTTTAAC 
CACATAGCAC ATATGGGTCC AGGAGATGGA ATACAGATCC TAAAAGACCT GCCCATGCCG 
GGTTTCCCTT ATCCATCCCC TCATCATCAC TCCGGTCTGA TCTCCTCTCC GATCAACTTT 
GAGCACATCT ATCACATGAC TGTTAATTCT GCTGAAAAAT TTCTCTCTCC TGATTCCATA 
AACCCTGAAT ATTCCCCGTC TCTGCGCTCT GTCCCTGGTA CACCAAGCTT TATGACTCTG 
AGGAACCCTC GGCCTCAGGA AAGTCGGACA GCATTCAGTG GCTCGGTCAG TATTCCGTCT 
ATCACCAAAT CCCGCCCTGA ACCCGGTCGC TCCATGAGCG CCAGCAGTGG CCTATCTGCA 
AGGTCATCTG CACAGAATGG CAGTGCGTTA AAGAGAGAAT TCTCTGGAGG CAGCTACAGT 
GCAAAGCGGC AGCCCATGCC CTCCCCATCG GAGGGCTCGC TGTCCTCAGG AGGCATGGAC 
CAGGGAAGTG ACGCCCCAGC GCGGGACTAT GACGGAGAGG ACTCTGATTC TCCGCGGCAT 
TCCACGGCTT CCAACAGTTC CAACCTGAGC AGTCCTCCCA GCCCGGTGTC GCCCCGGAAG 
ACCAAGAGCC TGTCTCTGGA GAGCACAGAC CGCGGGAGCT GGGACCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPA ( XM_025286026.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141811.1 Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X14, mRNA.

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CloneID	OBu66403
Clone ID Related Accession (Same CDS sequence)	XM_025286026.1
Accession Version	XM_025286026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	serine/threonine-protein kinase MRCK alpha isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGATCCC 
GTTGAGAACA CATATGTACG GAATCCGAAC GTCAAGTTTT ACATCCAGTC AAGGTCCACC 
TCTCCTTCCA CATCTAGTGA AGCTGAGCCA GTTAAGACTG TAGACTCTAC TCCACTTCCA 
GTTCATACAC CAACCTTAAG GAAAAAGGGA TGCCCTGGCT CAACTGGCTT TCCATCGAAG 
CGCAAGATTC ATCAGTTTTT TGTCAAATCT TTTACTACTC CCACCAGGTG TCATCAGTGT 
ACATCTTTGA TGGTGGGTTT GATAAGACAA GGCTGTTCTT GTGAAGTGTG TGGATTTTCA 
TGTCACATAA CTTGTGTAAA CAAAGCTCCA ACTGCTTGTC CAGTTCCTCC TGAACAGACA 
AAAGGTCCTC TGGGTATAGA TCCACAGAAA GGAATAGGAA CAGCATATGA AGGGCATGTC 
AGGATCCCTA AGCCAGCTGG AGTGAAGAAA GGATGGCAAA GAGCACTGGC TGTAGTCTGT 
GACTTCAAGC TCTTTCTGTA CGATATTGCC GAAGGAAAGG CATCTCAGCC CAGTGTTGTA 
GTCAGTCAAG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT CTTGGCTTCT 
GATGTTATTC ATGCAAGTCG AAAAGATATA CCCTGTATAT TTAGAGTCAC AGCTTCCCAG 
CTCTCAGCAT CTAGTAACAA ATGTTCCATC CTGATGCTAG CTGATAGTGA AAATGAGAAG 
AGTAAATGGG TGGGAGTACT GAGTGAATTG CACAAGATTT TGAAGAAAAA CAAATTCAGA 
GACCGCTCAG TCTATGTTCC CAAAGAGGCT TATGACAGCA CTCTGCCCCT CATTAAAACA 
ACCCAGGCAG CTGCAATCAT AGATCATGAA AGAATAGCTT TGGGGAATGA AGAAGGGTTA 
TTTGTTGTGC ATGTCACCAA AGATGAAATT ATTAGAGTTG GTGACAATAA GAAGATTCAT 
CAGATAGAAC TCATTCCAAA TGATCAACTT GTTGCCGTGA TCTCAGGACG AAATCGACAC 
GTCCGACTTT TTCCTATGTC AGCTCTGGAC GGGCGAGAGA CTGATTTTTA TAAGCTGGCA 
GAAACTAAAG GCTGTCAAAC AATAACTTCT GGCAAAGTGC GTCATGGAGC TCTTACATGC 
TTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACGCGT 
CACAGAAAAT TTAAGGAAAT TCAAGTCCCG TACAACGTCC AGTGGATGGC GATCTTCAGT 
GAGCAGCTCT GTGTGGGATT CCAGTCTGGA TTTCTAAGAT ATCCCCTGAA TGGAGAAGGA 
AGTCCATACA GTATGCTCCA TTCAAATGAC AATACACTCT CATTTATTGC ACATCAACCA 
ATGGATGCCA TCTGTGCGGT TGAAATCTCC AGTAAAGAAT ACCTGCTGTG TTTTAACAGC 
ATTGGAATAT ACACTGACTG CCAGGGCCGA AGATCTAGAC AACAGGAACT GATGTGGCCA 
GCGAATCCTT CCTCTTGTTG TTACAATGCA CCGTATCTCT CAGTGTACAG TGAAAACGCA 
GTTGACATCT TTGATGTGAA CTCCATGGAA TGGATTCAGA CTCTCCCACT CAAAAAGGTT 
CGCCCCTTAA ACAGTGAAGG ATCATTAAAT CTTTTAGGCT TGGAGACGAT TAGATTGATA 
TATTTCAAAA ATAAAATGGC AGAAGGGGAT GAACTGGTAG TTCCTGAAAC ATCGGATAAT 
AGCCGGAAGC AAATGGTTAG AAATATTAAC AATAAGAGGC GTTACTCTTT CAGAGTTCCG 
GAAGAGGAAA GAATGCAACA GAGGAGGGAG ATGTTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACATA TGGGTCCAGG AGATGGAATA 
CAGATCCTAA AAGACCTGCC CATGCCGGGT TTCCCTTATC CATCCCCTCA TCATCACTCC 
GGTCTGATCT CCTCTCCGAT CAACTTTGAG CACATCTATC ACATGACTGT TAATTCTGCT 
GAAAAATTTC TCTCTCCTGA TTCCATAAAC CCTGAATATT CCCCGTCTCT GCGCTCTGTC 
CCTGAACCCT CGGCCTCAGG AAAGTCGGAC AGCATTCAGT GGCTCGGTCA GTATTCCGTC 
TATCACCAAA TCCCGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141811.1
CDS	766..5946
Translation	MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPPQVSDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFNGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTPLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTADGPLTASKDLEIKSLKEEIEKLRKQVRESSHLEQQLEEANSVRRELDDAFRQIKAYEKQIKTLQQEREELNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVRTEAVAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKNRRESQSEREEFENEFKQQYEREKVLLTEENKKLTSELDKLTTLCENLSARNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFEDSFSSSSSSLIDFLDDRSPSCTPASKGRRTDPVENTYVRNPNVKFYIQSRSTSPSTSSEAEPVKTVDSTPLPVHTPTLRKKGCPGSTGFPSKRKIHQFFVKSFTTPTRCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTACPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAEGKASQPSVVVSQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASSNKCSILMLADSENEKSKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTITSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGSPYSMLHSNDNTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNSEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPEPSASGKSDSIQWLGQYSVYHQIPP

Target ORF information:

RefSeq Version	XM_025286026.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025286026.1

ORF Insert Sequence:

ATGTCTGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTGGACGG GCCCGCTCAG 
ACCAATGGGC AGTGCTTCAG TGTGGAAACG TTACTGGATA TCCTCATCTG CCTTTACGAT 
GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATACCT AGAATGGGCT 
AAACCATTTA CTTCTAAAGT GAAACAGATG CGATTACATA GAGAAGACTT TGAAATATTA 
AAGGTGATTG GTCGAGGGGC TTTTGGAGAG GTTGCTGTAG TGAAACTAAA AAATGCAGAT 
AAAGTATTTG CCATGAAAAT ATTGAATAAA TGGGAAATGC TGAAAAGAGC TGAGACAGCA 
TGTTTTCGTG AAGAAAGGGA TGTATTAGTG AATGGAGACA ATAAATGGAT TACAACTTTG 
CATTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 
GATTTGCTTA CTCTACTCAG CAAATTTGAA GATAGATTAC CTGAAGACAT GGCTCGATTT 
TACTTGGCTG AGATGGTGAT AGCAATTGAT TCAGTTCATC AGCTGCATTA TGTACACAGA 
GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGGTT AGCAGATTTT 
GGTTCATGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAAT CCTCAGTGGC TGTTGGAACT 
CCAGATTATA TCTCTCCTGA AATCCTTCAA GCCATGGAAG ATGGAAAAGG CAGATATGGA 
CCTGAGTGTG ACTGGTGGTC TTTGGGGGTC TGTATGTATG AAATGCTTTA TGGAGAAACA 
CCATTTTATG CAGAATCTCT GGTGGAAACA TATGGAAAAA TCATGAATCA CAAAGAGAGG 
TTTCAGTTTC CTCCTCAAGT GAGTGATGTG TCTGAAAATG CTAAGGATCT TATTCGAAGA 
CTCATCTGTA GCAGAGAACA TCGACTTGGT CAAAATGGAA TAGAAGACTT TAAGAAGCAC 
CCATTTTTCA ATGGAATTGA TTGGGATAAT ATTCGAAACT GTGAAGCACC TTATATTCCA 
GAAGTTAGTA GCCCAACAGA TACGTCGAAT TTTGATGTGG ATGATGATTG TTTAAAGAAT 
TCTGAAACGA TGCCCCCACC AACACATACT GCCTTTTCTG GTCACCATCT GCCATTTGTT 
GGTTTTACAT ATACCAGTAG CTGTGTTCTT TCTGATCGAA GCTGTTTAAG AGTTACAGCT 
GGTCCCACAC CATTGGATCT CGATGTTAAT GTGCAGAGGA CTCTAGATAA CAACTTAGCA 
ACTGAAGCTT ATGAAAGAAG AATTAAGCGC CTTGAGCAGG AAAAACTTGA ACTTAGTAGA 
AAGCTTCAAG AGTCAACGCA GACTGTCCAG GCTCTGCAGT ATTCAACTGC TGATGGTCCA 
CTAACAGCAA GCAAAGATTT AGAAATAAAA AGCTTAAAAG AAGAAATTGA AAAACTAAGG 
AAACAAGTAA GAGAATCAAG TCACTTGGAA CAGCAACTTG AAGAAGCTAA TTCTGTGAGG 
CGAGAATTAG ATGATGCTTT TAGACAAATC AAGGCTTATG AAAAACAAAT CAAGACATTA 
CAACAAGAAA GGGAAGAATT AAATAAGGAA CTAGTCCAGG CTAGTGAGCG ATTAAAAAAC 
CAATCCAAAG AGCTGAAAGA CGCACACTGT CAGAGGAAAC TGGCCATGCA GGAATTCATG 
GAGATCAATG AGCGGCTAAC AGAATTGCAC ACCCAAAAAC AGAAACTTGC TCGCCATGTC 
CGAGATAAGG AAGAAGAGGT GGACCTGGTG ATGCAAAAAG TTGAAAGCTT AAGGCAAGAA 
CTGCGCAGAA CAGAAAGAGC CAAAAAAGAG CTGGAAGTTC GTACAGAAGC TGTGGCTGCT 
GAAGCATCTA AAGACAGGAA ATTGCGTGAA CAGAGTGAGC ACTATTCTAA GCAACTGGAG 
AATGAACTAG AGGGACTGAA GCAAAAACAA ATTAGTTACT CACCAGGCGT ATGCAGCATA 
GAACATCAGC AAGAAATAAC CAAACTAAAG ACTGATTTGG AAAAGAAAAG TATCTTTTAT 
GAAGAAGAAT TGTCCAAGAG AGAAGGAATA CATGCAAATG AAATTAAAAA TCTGAAGAAA 
GAACTACATG ATTCAGAAGG CCAACAACTT GCTCTCAACA AAGAAATTAT GATTTTGAAA 
GACAAATTGG AAAAAAACAG GAGAGAGAGT CAAAGTGAAA GGGAAGAATT TGAAAATGAG 
TTCAAGCAAC AGTATGAACG AGAAAAAGTG TTACTAACTG AAGAAAATAA AAAACTGACA 
AGTGAGCTTG ATAAGCTTAC CACTCTGTGT GAGAACTTAA GTGCACGCAA CCAGCAGTTA 
GAAGAAGAGG TCAAAGATCT AGCAGATAAG AAGGAATCGG TTGCTCATTG GGAGGCTCAG 
ATCACAGAAA TAATTCAGTG GGTCAGTGAT GAAAAGGATG CACGAGGGTA TCTTCAAGCC 
TTAGCTTCTA AAATGACTGA AGAATTGGAA GCTTTAAGAA ACTCCAGCTT GGGGACCCGA 
GCAACAGATA TGCCCTGGAA AATGCGACGT TTTGCAAAAC TGGATATGTC AGCCAGACTG 
GAGTTACAGT CAGCTCTGGA TGCAGAAATA AGAGCCAAAC AGGCCATCCA AGAAGAGCTG 
AATAAAGTTA AAGCATCTAA CATCATAACA GAATGTAAAC TAAAAGATTC AGAGAAGAAG 
AACTTGGAAC TACTATCAGA AATCGAACAA CTGATAAAGG ACACTGAAGA GCTTAGATCT 
GAAAAGGGTA TAGAGCACCA AGACTCACAG CATTCTTTCT TGGCATTTTT GAATACGCCT 
ACCGATGCTC TGGATCAATT TGAAGATTCC TTTTCTTCTT CCTCATCTTC ACTGATTGAT 
TTTTTGGATG ACCGCTCTCC ATCCTGTACT CCAGCTAGCA AAGGCAGACG TACTGATCCC 
GTTGAGAACA CATATGTACG GAATCCGAAC GTCAAGTTTT ACATCCAGTC AAGGTCCACC 
TCTCCTTCCA CATCTAGTGA AGCTGAGCCA GTTAAGACTG TAGACTCTAC TCCACTTCCA 
GTTCATACAC CAACCTTAAG GAAAAAGGGA TGCCCTGGCT CAACTGGCTT TCCATCGAAG 
CGCAAGATTC ATCAGTTTTT TGTCAAATCT TTTACTACTC CCACCAGGTG TCATCAGTGT 
ACATCTTTGA TGGTGGGTTT GATAAGACAA GGCTGTTCTT GTGAAGTGTG TGGATTTTCA 
TGTCACATAA CTTGTGTAAA CAAAGCTCCA ACTGCTTGTC CAGTTCCTCC TGAACAGACA 
AAAGGTCCTC TGGGTATAGA TCCACAGAAA GGAATAGGAA CAGCATATGA AGGGCATGTC 
AGGATCCCTA AGCCAGCTGG AGTGAAGAAA GGATGGCAAA GAGCACTGGC TGTAGTCTGT 
GACTTCAAGC TCTTTCTGTA CGATATTGCC GAAGGAAAGG CATCTCAGCC CAGTGTTGTA 
GTCAGTCAAG TGATTGACAT GAGGGATGAA GAATTTTCTG TGAGTTCAGT CTTGGCTTCT 
GATGTTATTC ATGCAAGTCG AAAAGATATA CCCTGTATAT TTAGAGTCAC AGCTTCCCAG 
CTCTCAGCAT CTAGTAACAA ATGTTCCATC CTGATGCTAG CTGATAGTGA AAATGAGAAG 
AGTAAATGGG TGGGAGTACT GAGTGAATTG CACAAGATTT TGAAGAAAAA CAAATTCAGA 
GACCGCTCAG TCTATGTTCC CAAAGAGGCT TATGACAGCA CTCTGCCCCT CATTAAAACA 
ACCCAGGCAG CTGCAATCAT AGATCATGAA AGAATAGCTT TGGGGAATGA AGAAGGGTTA 
TTTGTTGTGC ATGTCACCAA AGATGAAATT ATTAGAGTTG GTGACAATAA GAAGATTCAT 
CAGATAGAAC TCATTCCAAA TGATCAACTT GTTGCCGTGA TCTCAGGACG AAATCGACAC 
GTCCGACTTT TTCCTATGTC AGCTCTGGAC GGGCGAGAGA CTGATTTTTA TAAGCTGGCA 
GAAACTAAAG GCTGTCAAAC AATAACTTCT GGCAAAGTGC GTCATGGAGC TCTTACATGC 
TTGTGTGTGG CTATGAAAAG GCAGGTCCTC TGTTATGAAC TATTTCAGAG CAAGACGCGT 
CACAGAAAAT TTAAGGAAAT TCAAGTCCCG TACAACGTCC AGTGGATGGC GATCTTCAGT 
GAGCAGCTCT GTGTGGGATT CCAGTCTGGA TTTCTAAGAT ATCCCCTGAA TGGAGAAGGA 
AGTCCATACA GTATGCTCCA TTCAAATGAC AATACACTCT CATTTATTGC ACATCAACCA 
ATGGATGCCA TCTGTGCGGT TGAAATCTCC AGTAAAGAAT ACCTGCTGTG TTTTAACAGC 
ATTGGAATAT ACACTGACTG CCAGGGCCGA AGATCTAGAC AACAGGAACT GATGTGGCCA 
GCGAATCCTT CCTCTTGTTG TTACAATGCA CCGTATCTCT CAGTGTACAG TGAAAACGCA 
GTTGACATCT TTGATGTGAA CTCCATGGAA TGGATTCAGA CTCTCCCACT CAAAAAGGTT 
CGCCCCTTAA ACAGTGAAGG ATCATTAAAT CTTTTAGGCT TGGAGACGAT TAGATTGATA 
TATTTCAAAA ATAAAATGGC AGAAGGGGAT GAACTGGTAG TTCCTGAAAC ATCGGATAAT 
AGCCGGAAGC AAATGGTTAG AAATATTAAC AATAAGAGGC GTTACTCTTT CAGAGTTCCG 
GAAGAGGAAA GAATGCAACA GAGGAGGGAG ATGTTACGAG ATCCAGAAAT GAGAAATAAA 
TTAATTTCTA ACCCAACTAA TTTTAACCAC ATAGCACATA TGGGTCCAGG AGATGGAATA 
CAGATCCTAA AAGACCTGCC CATGCCGGGT TTCCCTTATC CATCCCCTCA TCATCACTCC 
GGTCTGATCT CCTCTCCGAT CAACTTTGAG CACATCTATC ACATGACTGT TAATTCTGCT 
GAAAAATTTC TCTCTCCTGA TTCCATAAAC CCTGAATATT CCCCGTCTCT GCGCTCTGTC 
CCTGAACCCT CGGCCTCAGG AAAGTCGGAC AGCATTCAGT GGCTCGGTCA GTATTCCGTC 
TATCACCAAA TCCCGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CDC42BPA
Entrez Gene ID	102398286
Full Name	CDC42 binding protein kinase alpha
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CDC42BPA cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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