PLA2G4E cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLA2G4E
Entrez Gene ID	102398203
Full Name	phospholipase A2 group IVE
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006066213.2	XP_006066275.1	cytosolic phospholipase A2 epsilon
XM_006066213.1	XP_006066275.1	cytosolic phospholipase A2 epsilon

Pathways from BioSystems
Gene Ontology

The following PLA2G4E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLA2G4E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu21605	XM_006066213.2 Latest version!	Bubalus bubalis phospholipase A2 group IVE (PLA2G4E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OBu21605	XM_006066213.1	Bubalus bubalis phospholipase A2, group IVE (PLA2G4E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLA2G4E ( XM_006066213.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006066275.1 Bubalus bubalis phospholipase A2 group IVE (PLA2G4E), mRNA.

Price & Availability↑

CloneID	OBu21605
Clone ID Related Accession (Same CDS sequence)	XM_006066213.2 , XM_006066213.1
Accession Version	XM_006066213.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cytosolic phospholipase A2 epsilon
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006066213.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCTCC AGACCTCGGA AGGCCATCCT GGCTTGGGAA CGGATGTGTT TGGCCCTCAG 
AGCCTTCAGT GGGATGAAGA AGGCAGCATG GCAGGAAGGA GTGTCTGTGA GTCGGAGCAG 
ATACAGGTCC TGGAAACTCC CCATCTCACA TCTCTACTTC CCGTGGCCCC TGATGGCTCT 
CAGGACGGGC TGTCTCCATG CCACCTGCTG ACAGTGAGGG TCATCCGCAT GAAGAACGTC 
AGACAGGCCG ACGTGGTGAG CCTGACAGAC TGCTTTGTGA GCCTCTGGCT GCCCACCGCC 
AGTGAAAAGA GGCTGAGGAC TAGGACCATC TCCAACTGCC GAAACCCAGA ATGGAATGAG 
ACCTTCAGCT TCCAGATCCA GAGCCAAGTG AAGAACGTTC TAGAGCTGAG CGTCTGTGAT 
GAAGACACGG TGACACCTGA TGACCATCTC TTGACAGTTC TCTATGACCT CACCAAGCTC 
TGCTTCCGAA AGAAAACCCA CGTGAAGTTC CCACTCAACA CAGAGGGCAT GGAGGAGCTG 
GAAGTGGAGT TCCTGCTGGA GGAGAGCCCC TCTCCACCTG AGAGCCTCAT CACGAACGGC 
GTGCTGGTGT CTCGACAAAT GTCCTGCCTG GAGGTTTGTG CAGAATCCAG GAGGCCAAAG 
AAGAGGAAAA AGAAGGACCT CCTGGTGACA GTGACGGAGT CCTTCGAGCA CACCCAGCAC 
ATCTCACCCT GCCTGGAGTC CCGCTGCTCA AACCTTGCCT GCTTCCAATA CCCCAAATAC 
TTCCAGCCCG AGGTGCACGT GGAGTTGCCC AAGAGCCCAT GGATCTGTGA GCTTTGCTGC 
TGTGCACGCC AGAAGGGCCC TGTGTGCCAG TCCCTCCACT GCCTCCCCGA TGGCCAGGCG 
GTGACCTTGC CTGTGAATGA GAACTGTGAA TTACACATGA AGTCTACACC CTGTTCCCAG 
GCGCTGGATG TGCGGCTGGG CTTCAGCCTG TGCCAGGCAG AACTGGAGTT CCTGCAGAAA 
CGGAGGGTGG TGGCAGCCCA GGCGCTGAAG GAGGTGCTGC AGCTGGAGGA GGACCTGCGG 
GCAGATGAGG TCCCGCTGAT AGCCATCATG GCCACTGGGG GTGGAACAAG ATCTATGACC 
GCCATGTACG GCCACTTGCT GGCGCTGCAG AAGCTGAACA TCCTGAACTG TGCCAGCTAT 
ATCACCGGCC TGTCTGGGGC CACCTGGACC ATGGCCACCT TGTACCGTGA CCCTGACTGG 
TCCTCCAAAA ACCTGGAGCG TGCCGTCCTC GAGGCACGGA GGCATACGGT CAAGGACAAG 
ATGCCTGCCC TGTTCCCAGA CCAGCTCCTC AAATTCCAGG AGGAACTCCA GCAGCGCAGG 
CAGGAAGGCT ACAAGGTCAC CTTCACAGAC TTCTGGGGCC TGCTGATTGA GGCCTGTCTG 
GGGGATGAGA GAAATGAATG CAAACTGTCA GATCAGCGCG CTGCTTTGTG CCATGGCCAG 
AACCCCCTGC CCATCTACCT CACCATCAAT GTCAAGGATG ATGTAAGCAA CCAGGATTTC 
AGAGAGTGGT TCGAGTTCTC CCCCTACGAG GTGGGCATGC AGAAGTATGG AGCCTTCATC 
CCCACAGAGC TCTTCGGCTC CGAGTTCTTC ATGGGGCGGC TGATGAAGAG GATCCCAGAG 
TCGCGGATGT GCTACATGCT AGGTTTGTGG AGTAGCATCT TCTCCCTGAA CCTGCTTGAC 
GCCTGGAACC TATCCCACAC CTCGGAGGAG TTTTTTCACC AGTGGACAAG GGATAGAGTG 
CATGATATTG AAGACGAGCC ACTCCTGCCT GAAATCCCCA AATGTGATGC CAACGTCCTG 
GACACCACGG TGGTGACGCC AGCATCCTGG CTGTCCAACA CTTTCCGCGA CATCCTCACC 
CACAGGGCCT TTGTCTCCAA GTTCCACAAT TTCCTGCTGG GGCTGCAGCT GCACACCGAC 
TACCTCCAGA ACAGCCAGTT CTCCATGTGG AAAGACACGG TGCTGGACGG CTTCCCAAAC 
CAGCTGACAG GATCTGTGAA ACACCTGTGC CTGCTGGACA CCGCATTCTT TGTCAACTCC 
AGCTACGCGC CCCTCCTCAG GCCCGAGAGA AAAGTTGACC TCATCATCCA CCTTAATTAC 
TGTGCTGGGT CCCAGACAAA GCCCATGAAG CAAACCTGTG AGTACTGCAC TGTGCAGAAC 
ATCCCCTTCC TCAAATATGA GCTGCAGAAG GAGGAGGATA ACCTCAAGGA GTGCTACCTG 
CTGGAGAACT CCCAGGAGCC CGACACCCCC ATCGTGACCT TTTTCCCACT CATCTGCGAC 
ACCTTCCAAA ATTACAAGGC CCCAGGTGTG GAACGAAGCC CCGAGGAGCT GGAGCAGGGC 
CAGGTTGACA TCTACGGTCC CAAAAGTCCC TACGCCACCA AGGAGCTGAC ATACACTGAG 
GCCGCCTTCG ACAAGCTGGT GAAGCTTTCT GAGTATAACA TCCTGAATAA CAAGGACAAG 
CTCCTTCAGG CCTTGCGACT GGCTGTAGAG AGGAAACGTC AGAAAAGCCA GTGTCCTCCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006066275.1
CDS	333..2915
Translation	MTLQTSEGHPGLGTDVFGPQSLQWDEEGSMAGRSVCESEQIQVLETPHLTSLLPVAPDGSQDGLSPCHLLTVRVIRMKNVRQADVVSLTDCFVSLWLPTASEKRLRTRTISNCRNPEWNETFSFQIQSQVKNVLELSVCDEDTVTPDDHLLTVLYDLTKLCFRKKTHVKFPLNTEGMEELEVEFLLEESPSPPESLITNGVLVSRQMSCLEVCAESRRPKKRKKKDLLVTVTESFEHTQHISPCLESRCSNLACFQYPKYFQPEVHVELPKSPWICELCCCARQKGPVCQSLHCLPDGQAVTLPVNENCELHMKSTPCSQALDVRLGFSLCQAELEFLQKRRVVAAQALKEVLQLEEDLRADEVPLIAIMATGGGTRSMTAMYGHLLALQKLNILNCASYITGLSGATWTMATLYRDPDWSSKNLERAVLEARRHTVKDKMPALFPDQLLKFQEELQQRRQEGYKVTFTDFWGLLIEACLGDERNECKLSDQRAALCHGQNPLPIYLTINVKDDVSNQDFREWFEFSPYEVGMQKYGAFIPTELFGSEFFMGRLMKRIPESRMCYMLGLWSSIFSLNLLDAWNLSHTSEEFFHQWTRDRVHDIEDEPLLPEIPKCDANVLDTTVVTPASWLSNTFRDILTHRAFVSKFHNFLLGLQLHTDYLQNSQFSMWKDTVLDGFPNQLTGSVKHLCLLDTAFFVNSSYAPLLRPERKVDLIIHLNYCAGSQTKPMKQTCEYCTVQNIPFLKYELQKEEDNLKECYLLENSQEPDTPIVTFFPLICDTFQNYKAPGVERSPEELEQGQVDIYGPKSPYATKELTYTEAAFDKLVKLSEYNILNNKDKLLQALRLAVERKRQKSQCPP

Target ORF information:

RefSeq Version	XM_006066213.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phospholipase A2 group IVE (PLA2G4E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006066213.2

ORF Insert Sequence:

ATGACTCTCC AGACCTCGGA AGGCCATCCT GGCTTGGGAA CGGATGTGTT TGGCCCTCAG 
AGCCTTCAGT GGGATGAAGA AGGCAGCATG GCAGGAAGGA GTGTCTGTGA GTCGGAGCAG 
ATACAGGTCC TGGAAACTCC CCATCTCACA TCTCTACTTC CCGTGGCCCC TGATGGCTCT 
CAGGACGGGC TGTCTCCATG CCACCTGCTG ACAGTGAGGG TCATCCGCAT GAAGAACGTC 
AGACAGGCCG ACGTGGTGAG CCTGACAGAC TGCTTTGTGA GCCTCTGGCT GCCCACCGCC 
AGTGAAAAGA GGCTGAGGAC TAGGACCATC TCCAACTGCC GAAACCCAGA ATGGAATGAG 
ACCTTCAGCT TCCAGATCCA GAGCCAAGTG AAGAACGTTC TAGAGCTGAG CGTCTGTGAT 
GAAGACACGG TGACACCTGA TGACCATCTC TTGACAGTTC TCTATGACCT CACCAAGCTC 
TGCTTCCGAA AGAAAACCCA CGTGAAGTTC CCACTCAACA CAGAGGGCAT GGAGGAGCTG 
GAAGTGGAGT TCCTGCTGGA GGAGAGCCCC TCTCCACCTG AGAGCCTCAT CACGAACGGC 
GTGCTGGTGT CTCGACAAAT GTCCTGCCTG GAGGTTTGTG CAGAATCCAG GAGGCCAAAG 
AAGAGGAAAA AGAAGGACCT CCTGGTGACA GTGACGGAGT CCTTCGAGCA CACCCAGCAC 
ATCTCACCCT GCCTGGAGTC CCGCTGCTCA AACCTTGCCT GCTTCCAATA CCCCAAATAC 
TTCCAGCCCG AGGTGCACGT GGAGTTGCCC AAGAGCCCAT GGATCTGTGA GCTTTGCTGC 
TGTGCACGCC AGAAGGGCCC TGTGTGCCAG TCCCTCCACT GCCTCCCCGA TGGCCAGGCG 
GTGACCTTGC CTGTGAATGA GAACTGTGAA TTACACATGA AGTCTACACC CTGTTCCCAG 
GCGCTGGATG TGCGGCTGGG CTTCAGCCTG TGCCAGGCAG AACTGGAGTT CCTGCAGAAA 
CGGAGGGTGG TGGCAGCCCA GGCGCTGAAG GAGGTGCTGC AGCTGGAGGA GGACCTGCGG 
GCAGATGAGG TCCCGCTGAT AGCCATCATG GCCACTGGGG GTGGAACAAG ATCTATGACC 
GCCATGTACG GCCACTTGCT GGCGCTGCAG AAGCTGAACA TCCTGAACTG TGCCAGCTAT 
ATCACCGGCC TGTCTGGGGC CACCTGGACC ATGGCCACCT TGTACCGTGA CCCTGACTGG 
TCCTCCAAAA ACCTGGAGCG TGCCGTCCTC GAGGCACGGA GGCATACGGT CAAGGACAAG 
ATGCCTGCCC TGTTCCCAGA CCAGCTCCTC AAATTCCAGG AGGAACTCCA GCAGCGCAGG 
CAGGAAGGCT ACAAGGTCAC CTTCACAGAC TTCTGGGGCC TGCTGATTGA GGCCTGTCTG 
GGGGATGAGA GAAATGAATG CAAACTGTCA GATCAGCGCG CTGCTTTGTG CCATGGCCAG 
AACCCCCTGC CCATCTACCT CACCATCAAT GTCAAGGATG ATGTAAGCAA CCAGGATTTC 
AGAGAGTGGT TCGAGTTCTC CCCCTACGAG GTGGGCATGC AGAAGTATGG AGCCTTCATC 
CCCACAGAGC TCTTCGGCTC CGAGTTCTTC ATGGGGCGGC TGATGAAGAG GATCCCAGAG 
TCGCGGATGT GCTACATGCT AGGTTTGTGG AGTAGCATCT TCTCCCTGAA CCTGCTTGAC 
GCCTGGAACC TATCCCACAC CTCGGAGGAG TTTTTTCACC AGTGGACAAG GGATAGAGTG 
CATGATATTG AAGACGAGCC ACTCCTGCCT GAAATCCCCA AATGTGATGC CAACGTCCTG 
GACACCACGG TGGTGACGCC AGCATCCTGG CTGTCCAACA CTTTCCGCGA CATCCTCACC 
CACAGGGCCT TTGTCTCCAA GTTCCACAAT TTCCTGCTGG GGCTGCAGCT GCACACCGAC 
TACCTCCAGA ACAGCCAGTT CTCCATGTGG AAAGACACGG TGCTGGACGG CTTCCCAAAC 
CAGCTGACAG GATCTGTGAA ACACCTGTGC CTGCTGGACA CCGCATTCTT TGTCAACTCC 
AGCTACGCGC CCCTCCTCAG GCCCGAGAGA AAAGTTGACC TCATCATCCA CCTTAATTAC 
TGTGCTGGGT CCCAGACAAA GCCCATGAAG CAAACCTGTG AGTACTGCAC TGTGCAGAAC 
ATCCCCTTCC TCAAATATGA GCTGCAGAAG GAGGAGGATA ACCTCAAGGA GTGCTACCTG 
CTGGAGAACT CCCAGGAGCC CGACACCCCC ATCGTGACCT TTTTCCCACT CATCTGCGAC 
ACCTTCCAAA ATTACAAGGC CCCAGGTGTG GAACGAAGCC CCGAGGAGCT GGAGCAGGGC 
CAGGTTGACA TCTACGGTCC CAAAAGTCCC TACGCCACCA AGGAGCTGAC ATACACTGAG 
GCCGCCTTCG ACAAGCTGGT GAAGCTTTCT GAGTATAACA TCCTGAATAA CAAGGACAAG 
CTCCTTCAGG CCTTGCGACT GGCTGTAGAG AGGAAACGTC AGAAAAGCCA GTGTCCTCCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLA2G4E ( XM_006066213.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006066275.1 Bubalus bubalis phospholipase A2, group IVE (PLA2G4E), mRNA.

Price & Availability↑

CloneID	OBu21605
Clone ID Related Accession (Same CDS sequence)	XM_006066213.2 , XM_006066213.1
Accession Version	XM_006066213.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	cytosolic phospholipase A2 epsilon
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785199.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCTCC AGACCTCGGA AGGCCATCCT GGCTTGGGAA CGGATGTGTT TGGCCCTCAG 
AGCCTTCAGT GGGATGAAGA AGGCAGCATG GCAGGAAGGA GTGTCTGTGA GTCGGAGCAG 
ATACAGGTCC TGGAAACTCC CCATCTCACA TCTCTACTTC CCGTGGCCCC TGATGGCTCT 
CAGGACGGGC TGTCTCCATG CCACCTGCTG ACAGTGAGGG TCATCCGCAT GAAGAACGTC 
AGACAGGCCG ACGTGGTGAG CCTGACAGAC TGCTTTGTGA GCCTCTGGCT GCCCACCGCC 
AGTGAAAAGA GGCTGAGGAC TAGGACCATC TCCAACTGCC GAAACCCAGA ATGGAATGAG 
ACCTTCAGCT TCCAGATCCA GAGCCAAGTG AAGAACGTTC TAGAGCTGAG CGTCTGTGAT 
GAAGACACGG TGACACCTGA TGACCATCTC TTGACAGTTC TCTATGACCT CACCAAGCTC 
TGCTTCCGAA AGAAAACCCA CGTGAAGTTC CCACTCAACA CAGAGGGCAT GGAGGAGCTG 
GAAGTGGAGT TCCTGCTGGA GGAGAGCCCC TCTCCACCTG AGAGCCTCAT CACGAACGGC 
GTGCTGGTGT CTCGACAAAT GTCCTGCCTG GAGGTTTGTG CAGAATCCAG GAGGCCAAAG 
AAGAGGAAAA AGAAGGACCT CCTGGTGACA GTGACGGAGT CCTTCGAGCA CACCCAGCAC 
ATCTCACCCT GCCTGGAGTC CCGCTGCTCA AACCTTGCCT GCTTCCAATA CCCCAAATAC 
TTCCAGCCCG AGGTGCACGT GGAGTTGCCC AAGAGCCCAT GGATCTGTGA GCTTTGCTGC 
TGTGCACGCC AGAAGGGCCC TGTGTGCCAG TCCCTCCACT GCCTCCCCGA TGGCCAGGCG 
GTGACCTTGC CTGTGAATGA GAACTGTGAA TTACACATGA AGTCTACACC CTGTTCCCAG 
GCGCTGGATG TGCGGCTGGG CTTCAGCCTG TGCCAGGCAG AACTGGAGTT CCTGCAGAAA 
CGGAGGGTGG TGGCAGCCCA GGCGCTGAAG GAGGTGCTGC AGCTGGAGGA GGACCTGCGG 
GCAGATGAGG TCCCGCTGAT AGCCATCATG GCCACTGGGG GTGGAACAAG ATCTATGACC 
GCCATGTACG GCCACTTGCT GGCGCTGCAG AAGCTGAACA TCCTGAACTG TGCCAGCTAT 
ATCACCGGCC TGTCTGGGGC CACCTGGACC ATGGCCACCT TGTACCGTGA CCCTGACTGG 
TCCTCCAAAA ACCTGGAGCG TGCCGTCCTC GAGGCACGGA GGCATACGGT CAAGGACAAG 
ATGCCTGCCC TGTTCCCAGA CCAGCTCCTC AAATTCCAGG AGGAACTCCA GCAGCGCAGG 
CAGGAAGGCT ACAAGGTCAC CTTCACAGAC TTCTGGGGCC TGCTGATTGA GGCCTGTCTG 
GGGGATGAGA GAAATGAATG CAAACTGTCA GATCAGCGCG CTGCTTTGTG CCATGGCCAG 
AACCCCCTGC CCATCTACCT CACCATCAAT GTCAAGGATG ATGTAAGCAA CCAGGATTTC 
AGAGAGTGGT TCGAGTTCTC CCCCTACGAG GTGGGCATGC AGAAGTATGG AGCCTTCATC 
CCCACAGAGC TCTTCGGCTC CGAGTTCTTC ATGGGGCGGC TGATGAAGAG GATCCCAGAG 
TCGCGGATGT GCTACATGCT AGGTTTGTGG AGTAGCATCT TCTCCCTGAA CCTGCTTGAC 
GCCTGGAACC TATCCCACAC CTCGGAGGAG TTTTTTCACC AGTGGACAAG GGATAGAGTG 
CATGATATTG AAGACGAGCC ACTCCTGCCT GAAATCCCCA AATGTGATGC CAACGTCCTG 
GACACCACGG TGGTGACGCC AGCATCCTGG CTGTCCAACA CTTTCCGCGA CATCCTCACC 
CACAGGGCCT TTGTCTCCAA GTTCCACAAT TTCCTGCTGG GGCTGCAGCT GCACACCGAC 
TACCTCCAGA ACAGCCAGTT CTCCATGTGG AAAGACACGG TGCTGGACGG CTTCCCAAAC 
CAGCTGACAG GATCTGTGAA ACACCTGTGC CTGCTGGACA CCGCATTCTT TGTCAACTCC 
AGCTACGCGC CCCTCCTCAG GCCCGAGAGA AAAGTTGACC TCATCATCCA CCTTAATTAC 
TGTGCTGGGT CCCAGACAAA GCCCATGAAG CAAACCTGTG AGTACTGCAC TGTGCAGAAC 
ATCCCCTTCC TCAAATATGA GCTGCAGAAG GAGGAGGATA ACCTCAAGGA GTGCTACCTG 
CTGGAGAACT CCCAGGAGCC CGACACCCCC ATCGTGACCT TTTTCCCACT CATCTGCGAC 
ACCTTCCAAA ATTACAAGGC CCCAGGTGTG GAACGAAGCC CCGAGGAGCT GGAGCAGGGC 
CAGGTTGACA TCTACGGTCC CAAAAGTCCC TACGCCACCA AGGAGCTGAC ATACACTGAG 
GCCGCCTTCG ACAAGCTGGT GAAGCTTTCT GAGTATAACA TCCTGAATAA CAAGGACAAG 
CTCCTTCAGG CCTTGCGACT GGCTGTAGAG AGGAAACGTC AGAAAAGCCA GTGTCCTCCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006066275.1
CDS	299..2881
Translation	MTLQTSEGHPGLGTDVFGPQSLQWDEEGSMAGRSVCESEQIQVLETPHLTSLLPVAPDGSQDGLSPCHLLTVRVIRMKNVRQADVVSLTDCFVSLWLPTASEKRLRTRTISNCRNPEWNETFSFQIQSQVKNVLELSVCDEDTVTPDDHLLTVLYDLTKLCFRKKTHVKFPLNTEGMEELEVEFLLEESPSPPESLITNGVLVSRQMSCLEVCAESRRPKKRKKKDLLVTVTESFEHTQHISPCLESRCSNLACFQYPKYFQPEVHVELPKSPWICELCCCARQKGPVCQSLHCLPDGQAVTLPVNENCELHMKSTPCSQALDVRLGFSLCQAELEFLQKRRVVAAQALKEVLQLEEDLRADEVPLIAIMATGGGTRSMTAMYGHLLALQKLNILNCASYITGLSGATWTMATLYRDPDWSSKNLERAVLEARRHTVKDKMPALFPDQLLKFQEELQQRRQEGYKVTFTDFWGLLIEACLGDERNECKLSDQRAALCHGQNPLPIYLTINVKDDVSNQDFREWFEFSPYEVGMQKYGAFIPTELFGSEFFMGRLMKRIPESRMCYMLGLWSSIFSLNLLDAWNLSHTSEEFFHQWTRDRVHDIEDEPLLPEIPKCDANVLDTTVVTPASWLSNTFRDILTHRAFVSKFHNFLLGLQLHTDYLQNSQFSMWKDTVLDGFPNQLTGSVKHLCLLDTAFFVNSSYAPLLRPERKVDLIIHLNYCAGSQTKPMKQTCEYCTVQNIPFLKYELQKEEDNLKECYLLENSQEPDTPIVTFFPLICDTFQNYKAPGVERSPEELEQGQVDIYGPKSPYATKELTYTEAAFDKLVKLSEYNILNNKDKLLQALRLAVERKRQKSQCPP

Target ORF information:

RefSeq Version	XM_006066213.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phospholipase A2, group IVE (PLA2G4E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006066213.1

ORF Insert Sequence:

ATGACTCTCC AGACCTCGGA AGGCCATCCT GGCTTGGGAA CGGATGTGTT TGGCCCTCAG 
AGCCTTCAGT GGGATGAAGA AGGCAGCATG GCAGGAAGGA GTGTCTGTGA GTCGGAGCAG 
ATACAGGTCC TGGAAACTCC CCATCTCACA TCTCTACTTC CCGTGGCCCC TGATGGCTCT 
CAGGACGGGC TGTCTCCATG CCACCTGCTG ACAGTGAGGG TCATCCGCAT GAAGAACGTC 
AGACAGGCCG ACGTGGTGAG CCTGACAGAC TGCTTTGTGA GCCTCTGGCT GCCCACCGCC 
AGTGAAAAGA GGCTGAGGAC TAGGACCATC TCCAACTGCC GAAACCCAGA ATGGAATGAG 
ACCTTCAGCT TCCAGATCCA GAGCCAAGTG AAGAACGTTC TAGAGCTGAG CGTCTGTGAT 
GAAGACACGG TGACACCTGA TGACCATCTC TTGACAGTTC TCTATGACCT CACCAAGCTC 
TGCTTCCGAA AGAAAACCCA CGTGAAGTTC CCACTCAACA CAGAGGGCAT GGAGGAGCTG 
GAAGTGGAGT TCCTGCTGGA GGAGAGCCCC TCTCCACCTG AGAGCCTCAT CACGAACGGC 
GTGCTGGTGT CTCGACAAAT GTCCTGCCTG GAGGTTTGTG CAGAATCCAG GAGGCCAAAG 
AAGAGGAAAA AGAAGGACCT CCTGGTGACA GTGACGGAGT CCTTCGAGCA CACCCAGCAC 
ATCTCACCCT GCCTGGAGTC CCGCTGCTCA AACCTTGCCT GCTTCCAATA CCCCAAATAC 
TTCCAGCCCG AGGTGCACGT GGAGTTGCCC AAGAGCCCAT GGATCTGTGA GCTTTGCTGC 
TGTGCACGCC AGAAGGGCCC TGTGTGCCAG TCCCTCCACT GCCTCCCCGA TGGCCAGGCG 
GTGACCTTGC CTGTGAATGA GAACTGTGAA TTACACATGA AGTCTACACC CTGTTCCCAG 
GCGCTGGATG TGCGGCTGGG CTTCAGCCTG TGCCAGGCAG AACTGGAGTT CCTGCAGAAA 
CGGAGGGTGG TGGCAGCCCA GGCGCTGAAG GAGGTGCTGC AGCTGGAGGA GGACCTGCGG 
GCAGATGAGG TCCCGCTGAT AGCCATCATG GCCACTGGGG GTGGAACAAG ATCTATGACC 
GCCATGTACG GCCACTTGCT GGCGCTGCAG AAGCTGAACA TCCTGAACTG TGCCAGCTAT 
ATCACCGGCC TGTCTGGGGC CACCTGGACC ATGGCCACCT TGTACCGTGA CCCTGACTGG 
TCCTCCAAAA ACCTGGAGCG TGCCGTCCTC GAGGCACGGA GGCATACGGT CAAGGACAAG 
ATGCCTGCCC TGTTCCCAGA CCAGCTCCTC AAATTCCAGG AGGAACTCCA GCAGCGCAGG 
CAGGAAGGCT ACAAGGTCAC CTTCACAGAC TTCTGGGGCC TGCTGATTGA GGCCTGTCTG 
GGGGATGAGA GAAATGAATG CAAACTGTCA GATCAGCGCG CTGCTTTGTG CCATGGCCAG 
AACCCCCTGC CCATCTACCT CACCATCAAT GTCAAGGATG ATGTAAGCAA CCAGGATTTC 
AGAGAGTGGT TCGAGTTCTC CCCCTACGAG GTGGGCATGC AGAAGTATGG AGCCTTCATC 
CCCACAGAGC TCTTCGGCTC CGAGTTCTTC ATGGGGCGGC TGATGAAGAG GATCCCAGAG 
TCGCGGATGT GCTACATGCT AGGTTTGTGG AGTAGCATCT TCTCCCTGAA CCTGCTTGAC 
GCCTGGAACC TATCCCACAC CTCGGAGGAG TTTTTTCACC AGTGGACAAG GGATAGAGTG 
CATGATATTG AAGACGAGCC ACTCCTGCCT GAAATCCCCA AATGTGATGC CAACGTCCTG 
GACACCACGG TGGTGACGCC AGCATCCTGG CTGTCCAACA CTTTCCGCGA CATCCTCACC 
CACAGGGCCT TTGTCTCCAA GTTCCACAAT TTCCTGCTGG GGCTGCAGCT GCACACCGAC 
TACCTCCAGA ACAGCCAGTT CTCCATGTGG AAAGACACGG TGCTGGACGG CTTCCCAAAC 
CAGCTGACAG GATCTGTGAA ACACCTGTGC CTGCTGGACA CCGCATTCTT TGTCAACTCC 
AGCTACGCGC CCCTCCTCAG GCCCGAGAGA AAAGTTGACC TCATCATCCA CCTTAATTAC 
TGTGCTGGGT CCCAGACAAA GCCCATGAAG CAAACCTGTG AGTACTGCAC TGTGCAGAAC 
ATCCCCTTCC TCAAATATGA GCTGCAGAAG GAGGAGGATA ACCTCAAGGA GTGCTACCTG 
CTGGAGAACT CCCAGGAGCC CGACACCCCC ATCGTGACCT TTTTCCCACT CATCTGCGAC 
ACCTTCCAAA ATTACAAGGC CCCAGGTGTG GAACGAAGCC CCGAGGAGCT GGAGCAGGGC 
CAGGTTGACA TCTACGGTCC CAAAAGTCCC TACGCCACCA AGGAGCTGAC ATACACTGAG 
GCCGCCTTCG ACAAGCTGGT GAAGCTTTCT GAGTATAACA TCCTGAATAA CAAGGACAAG 
CTCCTTCAGG CCTTGCGACT GGCTGTAGAG AGGAAACGTC AGAAAAGCCA GTGTCCTCCC 
TAA