DHX30 gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_025272643.1	XP_025128428.1	putative ATP-dependent RNA helicase DHX30 isoform X1
XM_025272650.1	XP_025128435.1	putative ATP-dependent RNA helicase DHX30 isoform X5
XM_006065227.1	XP_006065289.1	putative ATP-dependent RNA helicase DHX30
XM_025272646.1	XP_025128431.1	putative ATP-dependent RNA helicase DHX30 isoform X3
XM_025272647.1	XP_025128432.1	putative ATP-dependent RNA helicase DHX30 isoform X4
XM_025272648.1	XP_025128433.1	putative ATP-dependent RNA helicase DHX30 isoform X4
XM_025272644.1	XP_025128429.1	putative ATP-dependent RNA helicase DHX30 isoform X2
XM_025272645.1	XP_025128430.1	putative ATP-dependent RNA helicase DHX30 isoform X3
XM_025272651.1	XP_025128436.1	putative ATP-dependent RNA helicase DHX30 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu57928	XM_025272643.1 Latest version!	Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu57929	XM_025272644.1 Latest version!	Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu57930	XM_025272646.1 Latest version!	Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu57930	XM_025272645.1 Latest version!	Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu57931	XM_025272647.1 Latest version!	Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu57931	XM_025272648.1 Latest version!	Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu57932	XM_025272650.1 Latest version!	Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu57933	XM_025272651.1 Latest version!	Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu20799	XM_006065227.1 Latest version!	Bubalus bubalis DEAH (Asp-Glu-Ala-His) box helicase 30 (DHX30), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

DHX30 ( XM_025272643.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025128428.1 Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu57928
Clone ID Related Accession (Same CDS sequence)	XM_025272643.1
Accession Version	XM_025272643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	putative ATP-dependent RNA helicase DHX30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGA AAGATTCGTC CCCAGGATTC CAGCTTTCCC TCCTGGCCAG AAATGTTCAG 
CCTGGACTCA TTCAGGAAAG ATCGGGCCCA ACACAGGCAG CGTCAGTGCA AACTTCCCCC 
ACCCCGTCTT CCACCCATGT GTGTCAACCC TGCCCCTGGA GGGACCATCT CTCGAGGCTG 
AACGTTAACA TTTCCAACAT GGCAGCTTCT AGGGACCTAT TAAAAGAGTT TCCACAGCCC 
AAAAATCTTC TCAACAGTGT GATTGGAAGA GCACTTGGCA TCTCACACGC AAAAGACAAA 
TTGGTCTACG TGCATACAAA TGGACCGAAG AAAAAGAAAG TCACCCTCCA CATAAAGTGG 
CCCAAGAGCG TGGAGGTAGA AGGCTATGGC AGCAAGAAGA TCGATGCCGA GAGGCAGGCT 
GCAGCTGCGG CCTGCCAGCT GTTCAAGGGC TGGGGTCTGC TGGGTCCCCG GAATGAGCTG 
TTTGATGCAG CCAAATACCG CGTGCTAGCC GATCGCTTTG GCTCTCCGGC TGACAGCTGG 
TGGCGCCCAG AACCCACCAT GCCACCCACT TCCTGGCGGC AGCTGAATCC TGAGAGCATC 
CGGCCAGGGG GACCTGGGAG CCTGTCCCGC TCCTTGGGTC GGGAGGAAGA GGAGGATGAG 
GAGGAAGAAC TAGAAGAAGG GACCATCGAT GTCACTGAAT TTCTGTCTAT GACCCAGCAG 
GACTCCCACG CCCCACTCAG GGATTCCAGG GGGGGTTCCT TTGAAATGAC AGATGACGAC 
AGTGCTATCA GGGCTCTGAC CCAGTTTCCA CTTCCCAAGA ACCTTCTGGC CAAGGTGATT 
CAGATAGCAA CATCCTCCTC CACAGCCAAG AACCTCATGC AGTTCCACAC CGTGGGCACC 
AAGACCAAGC TGTCCACCCT CACCCTGCTC TGGCCCTGTC CCATGACCTT CGTGGCCAAG 
GGGCGTCGCA AAGCAGAGGC AGAGAATAAA GCGGCAGCCT TGGCTTGTAA GAAACTGAAG 
AGCCTGGGAC TGGTGGACCG GAACAACGAG CCGCTCACCC ACGCCATGTA TAACTTGGCC 
TCCTTGCGAG AGCTGGGTGA GACCCAGCGC CGGCCGTGTA CCATCCAGGT GCCTGAGCCC 
ATCCTCCGCA AGATCGAGAC CTTCCTTAAC CATTACCCTG TGGAGAGTTC GTGGATCTCC 
TCAGAGCTCC GGCTGCAGGG TGAGGACATC CTGCCCTTGG GCAAGGACTC GGGGCCCCTG 
AGTGACCCCA TCACAGGCAA GCCCTATGTG CCACTGTCAG AAGCAGAGGA GCTGCGTCTG 
AGCCAGAGTC TGCTGGAGCT GTGGCGGCGG CGAGGGCCAG TCTGGCAGGA GGCACCCCAG 
CTCCCCGTGG ACCCTCATCG GGACACCATC CTCAATGCCA TCGAGCAGCA CCCAGTGGTG 
GTCATTGCTG GGGACACGGG CTGTGGGAAG ACCACGCGCA TCCCCCAGCT GCTGCTGGAG 
CGCTACGTGA CCGAGGGCCG CGGTGCGCGC TGCAACGTGA TCATCACCCA GCCACGCCGC 
ATCTCGGCCG TGTCTGTGGC GCAGCGGGTC AGCCACGAAC TGGGCCCTTC CCTGCGCCGG 
AACGTAGGCT TCCAGGTGCG GTTGGAAAGC AAGCCTCCGG CCCGAGGAGG GGCCCTGCTG 
TTCTGCACGG TGGGCATCCT GCTGCGGAAG CTGCAGAGCA ACCCCAGCCT GGAGGGCGTG 
AGCCACGTGG TCGTGGACGA GGTGCACGAG CGAGACGTGA ACACGGACTT CCTGCTGATC 
CTGCTCAAGG GCCTGCAGCG GCTCAACCCG GCTCTGCGCT TGGTGCTCAT GAGCGCCACT 
GGCGACAACG AGCGTTTCTC CCGCTACTTT GGTGGCTGCC CTGTCATCAA GGTCCCCGGC 
TTCATGTACC CTGTCAAGGA GCACTACCTG GAGGACATCC TGGCCAAGCT GGGCAAGCAC 
CAGTATCCGC ACCGGCACCG GCACCACGAG TCTGAGGATG AATGCGCTCT CGATTTGGAC 
CTCGTGACTG ATCTGGTTCT GCACATCGAT GCCCGCGGGG AACCAGGTGG GATCCTCTGC 
TTCCTGCCGG GCTGGCAGGA GATCAAAGGA GTCCAGCAAC GCCTCCAGGA GGCCCTGGGC 
ATGCACGAGA GCAAGTACCT CATCCTGCCA GTGCACTCCA ACATCCCGAT GATGGACCAG 
AAGGCCATAT TCCAGCAGCC TCCCATTGGG GTACGCAAGA TCGTCTTGGC CACCAACATT 
GCCGAGACGT CCATCACGAT CAATGACATC GTGCACGTGG TGGACAGCGG TCTGCACAAG 
GAGGAGCGCT ACGACCTGAA GACCAAGGTT TCCTGCCTGG AGACCGTGTG GGTGTCACGA 
GCCAATGTGA TCCAGCGCCG GGGCCGGGCG GGCCGCTGCC AGTCTGGCTT TGCCTACCAC 
CTCTTCCCAC GGAGCCGGCT GGAGAAGATG GCTCCTTTCC AAGTGCCAGA GATCCTGCGC 
ACACCCCTGG AGAACCTGGT GCTGCAGGCC AAGATCCACA TGCCAGAGAA GACGGCAGTG 
GAGTTCCTCT CCAAGGCCGT AGACAGTCCA AACATCAAGG CGGTGGACGA GGCCGTGATC 
TTGCTCCAAG AGATTGGGGT TCTGGACCAG CGGGAGTACC TGACCACCCT GGGGCAGCGC 
CTGGCCCACA TCTCCACTGA CCCGCGGCTG GCCAAGGCCA TCGTGCTGGC TGCCATCTTC 
CGTTGCCTGC ACCCGCTGTT AGTGGTCGTC TCGTGCCTCA CCCGGGACCC CTTCAGCAGC 
AGCCTGCAGA ACCGGGCGGA GGTGGACAAG GTGAAAGCAC TGTTGAGCCA CGACAGCGGC 
AGTGACCACC TGGCCTTTGT GCGGGCGGTG TCCGGCTGGG AGGAGGTGCT GCGCTGGCAG 
GATCGCAGCT CCCGTGAGAA CTACCTGGAG GAAAACCTGC TCTACGCACC CAGCCTGCGC 
TTCATCCACG GACTCATCAA GCAGTTCTCA GAGAACATTT ATGAGGCTTT CCTGGTTGGG 
AAGCCCTCGG ACTGCACCCT GGCCTCTGCC CAGTGCAACG AGTACAGTGA GGAGGAGGAG 
CTGGTGAAGG GGGTGCTGAT GGCGGGTCTC TACCCCAACC TCATCCAGGT GAGGCAGGGC 
AAGGTGACCC GGCAGGGCAA GTTCAAGCCC AACAGTGTCA CGTACAGGAC CAAATCCGGC 
AACATCTTGC TGCACAAGTC GACCATTAAC AGGGAGGCCA CCCGGCTGCG GAGCCGATGG 
CTGACGTATT TCATGGCTGT CAAGTCCAAC GGCAGCGTCT TCGTCCGGGA CTCCTCCCAG 
GTGCACCCAC TAGCTGTGCT GCTACTGACC GACGGGGACG TCCACATCCG TGATGACGGG 
CGCCGGGCCA CCATTTCCCT GAGCGACAGT GACCTGCTGC GGCTGGAGGG TGACTCGCGC 
ACCGTGCGGC TGCTGCGGGA GCTGCGCCGG GCCCTGGGCC GCATGGTGGA GCGGAGCCTG 
CGCAGCGAGC TGGCGGCACT GCCGCCCTGT GTGCAGGAGG AGCATGGGCA GCTGCTCGCC 
CTGCTGGCGG AGCTGCTGCG CGGGCCCTGC GGCAGCTTTG ATGTGCGCAA GACCGCTGAC 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025128428.1
CDS	207..3872
Translation	MDLKDSSPGFQLSLLARNVQPGLIQERSGPTQAASVQTSPTPSSTHVCQPCPWRDHLSRLNVNISNMAASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGSLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHAPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWISSELRLQGEDILPLGKDSGPLSDPITGKPYVPLSEAEELRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVIAGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVVVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPIGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMAPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVSGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLRELRRALGRMVERSLRSELAALPPCVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_025272643.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025272643.1

ORF Insert Sequence:

ATGGACCTGA AAGATTCGTC CCCAGGATTC CAGCTTTCCC TCCTGGCCAG AAATGTTCAG 
CCTGGACTCA TTCAGGAAAG ATCGGGCCCA ACACAGGCAG CGTCAGTGCA AACTTCCCCC 
ACCCCGTCTT CCACCCATGT GTGTCAACCC TGCCCCTGGA GGGACCATCT CTCGAGGCTG 
AACGTTAACA TTTCCAACAT GGCAGCTTCT AGGGACCTAT TAAAAGAGTT TCCACAGCCC 
AAAAATCTTC TCAACAGTGT GATTGGAAGA GCACTTGGCA TCTCACACGC AAAAGACAAA 
TTGGTCTACG TGCATACAAA TGGACCGAAG AAAAAGAAAG TCACCCTCCA CATAAAGTGG 
CCCAAGAGCG TGGAGGTAGA AGGCTATGGC AGCAAGAAGA TCGATGCCGA GAGGCAGGCT 
GCAGCTGCGG CCTGCCAGCT GTTCAAGGGC TGGGGTCTGC TGGGTCCCCG GAATGAGCTG 
TTTGATGCAG CCAAATACCG CGTGCTAGCC GATCGCTTTG GCTCTCCGGC TGACAGCTGG 
TGGCGCCCAG AACCCACCAT GCCACCCACT TCCTGGCGGC AGCTGAATCC TGAGAGCATC 
CGGCCAGGGG GACCTGGGAG CCTGTCCCGC TCCTTGGGTC GGGAGGAAGA GGAGGATGAG 
GAGGAAGAAC TAGAAGAAGG GACCATCGAT GTCACTGAAT TTCTGTCTAT GACCCAGCAG 
GACTCCCACG CCCCACTCAG GGATTCCAGG GGGGGTTCCT TTGAAATGAC AGATGACGAC 
AGTGCTATCA GGGCTCTGAC CCAGTTTCCA CTTCCCAAGA ACCTTCTGGC CAAGGTGATT 
CAGATAGCAA CATCCTCCTC CACAGCCAAG AACCTCATGC AGTTCCACAC CGTGGGCACC 
AAGACCAAGC TGTCCACCCT CACCCTGCTC TGGCCCTGTC CCATGACCTT CGTGGCCAAG 
GGGCGTCGCA AAGCAGAGGC AGAGAATAAA GCGGCAGCCT TGGCTTGTAA GAAACTGAAG 
AGCCTGGGAC TGGTGGACCG GAACAACGAG CCGCTCACCC ACGCCATGTA TAACTTGGCC 
TCCTTGCGAG AGCTGGGTGA GACCCAGCGC CGGCCGTGTA CCATCCAGGT GCCTGAGCCC 
ATCCTCCGCA AGATCGAGAC CTTCCTTAAC CATTACCCTG TGGAGAGTTC GTGGATCTCC 
TCAGAGCTCC GGCTGCAGGG TGAGGACATC CTGCCCTTGG GCAAGGACTC GGGGCCCCTG 
AGTGACCCCA TCACAGGCAA GCCCTATGTG CCACTGTCAG AAGCAGAGGA GCTGCGTCTG 
AGCCAGAGTC TGCTGGAGCT GTGGCGGCGG CGAGGGCCAG TCTGGCAGGA GGCACCCCAG 
CTCCCCGTGG ACCCTCATCG GGACACCATC CTCAATGCCA TCGAGCAGCA CCCAGTGGTG 
GTCATTGCTG GGGACACGGG CTGTGGGAAG ACCACGCGCA TCCCCCAGCT GCTGCTGGAG 
CGCTACGTGA CCGAGGGCCG CGGTGCGCGC TGCAACGTGA TCATCACCCA GCCACGCCGC 
ATCTCGGCCG TGTCTGTGGC GCAGCGGGTC AGCCACGAAC TGGGCCCTTC CCTGCGCCGG 
AACGTAGGCT TCCAGGTGCG GTTGGAAAGC AAGCCTCCGG CCCGAGGAGG GGCCCTGCTG 
TTCTGCACGG TGGGCATCCT GCTGCGGAAG CTGCAGAGCA ACCCCAGCCT GGAGGGCGTG 
AGCCACGTGG TCGTGGACGA GGTGCACGAG CGAGACGTGA ACACGGACTT CCTGCTGATC 
CTGCTCAAGG GCCTGCAGCG GCTCAACCCG GCTCTGCGCT TGGTGCTCAT GAGCGCCACT 
GGCGACAACG AGCGTTTCTC CCGCTACTTT GGTGGCTGCC CTGTCATCAA GGTCCCCGGC 
TTCATGTACC CTGTCAAGGA GCACTACCTG GAGGACATCC TGGCCAAGCT GGGCAAGCAC 
CAGTATCCGC ACCGGCACCG GCACCACGAG TCTGAGGATG AATGCGCTCT CGATTTGGAC 
CTCGTGACTG ATCTGGTTCT GCACATCGAT GCCCGCGGGG AACCAGGTGG GATCCTCTGC 
TTCCTGCCGG GCTGGCAGGA GATCAAAGGA GTCCAGCAAC GCCTCCAGGA GGCCCTGGGC 
ATGCACGAGA GCAAGTACCT CATCCTGCCA GTGCACTCCA ACATCCCGAT GATGGACCAG 
AAGGCCATAT TCCAGCAGCC TCCCATTGGG GTACGCAAGA TCGTCTTGGC CACCAACATT 
GCCGAGACGT CCATCACGAT CAATGACATC GTGCACGTGG TGGACAGCGG TCTGCACAAG 
GAGGAGCGCT ACGACCTGAA GACCAAGGTT TCCTGCCTGG AGACCGTGTG GGTGTCACGA 
GCCAATGTGA TCCAGCGCCG GGGCCGGGCG GGCCGCTGCC AGTCTGGCTT TGCCTACCAC 
CTCTTCCCAC GGAGCCGGCT GGAGAAGATG GCTCCTTTCC AAGTGCCAGA GATCCTGCGC 
ACACCCCTGG AGAACCTGGT GCTGCAGGCC AAGATCCACA TGCCAGAGAA GACGGCAGTG 
GAGTTCCTCT CCAAGGCCGT AGACAGTCCA AACATCAAGG CGGTGGACGA GGCCGTGATC 
TTGCTCCAAG AGATTGGGGT TCTGGACCAG CGGGAGTACC TGACCACCCT GGGGCAGCGC 
CTGGCCCACA TCTCCACTGA CCCGCGGCTG GCCAAGGCCA TCGTGCTGGC TGCCATCTTC 
CGTTGCCTGC ACCCGCTGTT AGTGGTCGTC TCGTGCCTCA CCCGGGACCC CTTCAGCAGC 
AGCCTGCAGA ACCGGGCGGA GGTGGACAAG GTGAAAGCAC TGTTGAGCCA CGACAGCGGC 
AGTGACCACC TGGCCTTTGT GCGGGCGGTG TCCGGCTGGG AGGAGGTGCT GCGCTGGCAG 
GATCGCAGCT CCCGTGAGAA CTACCTGGAG GAAAACCTGC TCTACGCACC CAGCCTGCGC 
TTCATCCACG GACTCATCAA GCAGTTCTCA GAGAACATTT ATGAGGCTTT CCTGGTTGGG 
AAGCCCTCGG ACTGCACCCT GGCCTCTGCC CAGTGCAACG AGTACAGTGA GGAGGAGGAG 
CTGGTGAAGG GGGTGCTGAT GGCGGGTCTC TACCCCAACC TCATCCAGGT GAGGCAGGGC 
AAGGTGACCC GGCAGGGCAA GTTCAAGCCC AACAGTGTCA CGTACAGGAC CAAATCCGGC 
AACATCTTGC TGCACAAGTC GACCATTAAC AGGGAGGCCA CCCGGCTGCG GAGCCGATGG 
CTGACGTATT TCATGGCTGT CAAGTCCAAC GGCAGCGTCT TCGTCCGGGA CTCCTCCCAG 
GTGCACCCAC TAGCTGTGCT GCTACTGACC GACGGGGACG TCCACATCCG TGATGACGGG 
CGCCGGGCCA CCATTTCCCT GAGCGACAGT GACCTGCTGC GGCTGGAGGG TGACTCGCGC 
ACCGTGCGGC TGCTGCGGGA GCTGCGCCGG GCCCTGGGCC GCATGGTGGA GCGGAGCCTG 
CGCAGCGAGC TGGCGGCACT GCCGCCCTGT GTGCAGGAGG AGCATGGGCA GCTGCTCGCC 
CTGCTGGCGG AGCTGCTGCG CGGGCCCTGC GGCAGCTTTG ATGTGCGCAA GACCGCTGAC 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_025272644.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025128429.1 Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu57929
Clone ID Related Accession (Same CDS sequence)	XM_025272644.1
Accession Version	XM_025272644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	putative ATP-dependent RNA helicase DHX30 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCG CCAGGAGACT CATGGCGCTG GCCGCCGGCG TCTCTCCGCG CCTGCGGCCA 
TCGGGTCCCC GCGCCATCGG GCGACAGGGA GGCCCGCGCG GCCTCTCCAC AGGCTGCTCC 
CGCCCCGACC GCACCCAGGA GGCCGCCGAG GCCGAGGCGG CCTCCGGCGA GCCTGGGGAA 
GGCGATGGAA GCGTGGTGAA CGCTTCTAGG GACCTATTAA AAGAGTTTCC ACAGCCCAAA 
AATCTTCTCA ACAGTGTGAT TGGAAGAGCA CTTGGCATCT CACACGCAAA AGACAAATTG 
GTCTACGTGC ATACAAATGG ACCGAAGAAA AAGAAAGTCA CCCTCCACAT AAAGTGGCCC 
AAGAGCGTGG AGGTAGAAGG CTATGGCAGC AAGAAGATCG ATGCCGAGAG GCAGGCTGCA 
GCTGCGGCCT GCCAGCTGTT CAAGGGCTGG GGTCTGCTGG GTCCCCGGAA TGAGCTGTTT 
GATGCAGCCA AATACCGCGT GCTAGCCGAT CGCTTTGGCT CTCCGGCTGA CAGCTGGTGG 
CGCCCAGAAC CCACCATGCC ACCCACTTCC TGGCGGCAGC TGAATCCTGA GAGCATCCGG 
CCAGGGGGAC CTGGGAGCCT GTCCCGCTCC TTGGGTCGGG AGGAAGAGGA GGATGAGGAG 
GAAGAACTAG AAGAAGGGAC CATCGATGTC ACTGAATTTC TGTCTATGAC CCAGCAGGAC 
TCCCACGCCC CACTCAGGGA TTCCAGGGGG GGTTCCTTTG AAATGACAGA TGACGACAGT 
GCTATCAGGG CTCTGACCCA GTTTCCACTT CCCAAGAACC TTCTGGCCAA GGTGATTCAG 
ATAGCAACAT CCTCCTCCAC AGCCAAGAAC CTCATGCAGT TCCACACCGT GGGCACCAAG 
ACCAAGCTGT CCACCCTCAC CCTGCTCTGG CCCTGTCCCA TGACCTTCGT GGCCAAGGGG 
CGTCGCAAAG CAGAGGCAGA GAATAAAGCG GCAGCCTTGG CTTGTAAGAA ACTGAAGAGC 
CTGGGACTGG TGGACCGGAA CAACGAGCCG CTCACCCACG CCATGTATAA CTTGGCCTCC 
TTGCGAGAGC TGGGTGAGAC CCAGCGCCGG CCGTGTACCA TCCAGGTGCC TGAGCCCATC 
CTCCGCAAGA TCGAGACCTT CCTTAACCAT TACCCTGTGG AGAGTTCGTG GATCTCCTCA 
GAGCTCCGGC TGCAGGGTGA GGACATCCTG CCCTTGGGCA AGGACTCGGG GCCCCTGAGT 
GACCCCATCA CAGGCAAGCC CTATGTGCCA CTGTCAGAAG CAGAGGAGCT GCGTCTGAGC 
CAGAGTCTGC TGGAGCTGTG GCGGCGGCGA GGGCCAGTCT GGCAGGAGGC ACCCCAGCTC 
CCCGTGGACC CTCATCGGGA CACCATCCTC AATGCCATCG AGCAGCACCC AGTGGTGGTC 
ATTGCTGGGG ACACGGGCTG TGGGAAGACC ACGCGCATCC CCCAGCTGCT GCTGGAGCGC 
TACGTGACCG AGGGCCGCGG TGCGCGCTGC AACGTGATCA TCACCCAGCC ACGCCGCATC 
TCGGCCGTGT CTGTGGCGCA GCGGGTCAGC CACGAACTGG GCCCTTCCCT GCGCCGGAAC 
GTAGGCTTCC AGGTGCGGTT GGAAAGCAAG CCTCCGGCCC GAGGAGGGGC CCTGCTGTTC 
TGCACGGTGG GCATCCTGCT GCGGAAGCTG CAGAGCAACC CCAGCCTGGA GGGCGTGAGC 
CACGTGGTCG TGGACGAGGT GCACGAGCGA GACGTGAACA CGGACTTCCT GCTGATCCTG 
CTCAAGGGCC TGCAGCGGCT CAACCCGGCT CTGCGCTTGG TGCTCATGAG CGCCACTGGC 
GACAACGAGC GTTTCTCCCG CTACTTTGGT GGCTGCCCTG TCATCAAGGT CCCCGGCTTC 
ATGTACCCTG TCAAGGAGCA CTACCTGGAG GACATCCTGG CCAAGCTGGG CAAGCACCAG 
TATCCGCACC GGCACCGGCA CCACGAGTCT GAGGATGAAT GCGCTCTCGA TTTGGACCTC 
GTGACTGATC TGGTTCTGCA CATCGATGCC CGCGGGGAAC CAGGTGGGAT CCTCTGCTTC 
CTGCCGGGCT GGCAGGAGAT CAAAGGAGTC CAGCAACGCC TCCAGGAGGC CCTGGGCATG 
CACGAGAGCA AGTACCTCAT CCTGCCAGTG CACTCCAACA TCCCGATGAT GGACCAGAAG 
GCCATATTCC AGCAGCCTCC CATTGGGGTA CGCAAGATCG TCTTGGCCAC CAACATTGCC 
GAGACGTCCA TCACGATCAA TGACATCGTG CACGTGGTGG ACAGCGGTCT GCACAAGGAG 
GAGCGCTACG ACCTGAAGAC CAAGGTTTCC TGCCTGGAGA CCGTGTGGGT GTCACGAGCC 
AATGTGATCC AGCGCCGGGG CCGGGCGGGC CGCTGCCAGT CTGGCTTTGC CTACCACCTC 
TTCCCACGGA GCCGGCTGGA GAAGATGGCT CCTTTCCAAG TGCCAGAGAT CCTGCGCACA 
CCCCTGGAGA ACCTGGTGCT GCAGGCCAAG ATCCACATGC CAGAGAAGAC GGCAGTGGAG 
TTCCTCTCCA AGGCCGTAGA CAGTCCAAAC ATCAAGGCGG TGGACGAGGC CGTGATCTTG 
CTCCAAGAGA TTGGGGTTCT GGACCAGCGG GAGTACCTGA CCACCCTGGG GCAGCGCCTG 
GCCCACATCT CCACTGACCC GCGGCTGGCC AAGGCCATCG TGCTGGCTGC CATCTTCCGT 
TGCCTGCACC CGCTGTTAGT GGTCGTCTCG TGCCTCACCC GGGACCCCTT CAGCAGCAGC 
CTGCAGAACC GGGCGGAGGT GGACAAGGTG AAAGCACTGT TGAGCCACGA CAGCGGCAGT 
GACCACCTGG CCTTTGTGCG GGCGGTGTCC GGCTGGGAGG AGGTGCTGCG CTGGCAGGAT 
CGCAGCTCCC GTGAGAACTA CCTGGAGGAA AACCTGCTCT ACGCACCCAG CCTGCGCTTC 
ATCCACGGAC TCATCAAGCA GTTCTCAGAG AACATTTATG AGGCTTTCCT GGTTGGGAAG 
CCCTCGGACT GCACCCTGGC CTCTGCCCAG TGCAACGAGT ACAGTGAGGA GGAGGAGCTG 
GTGAAGGGGG TGCTGATGGC GGGTCTCTAC CCCAACCTCA TCCAGGTGAG GCAGGGCAAG 
GTGACCCGGC AGGGCAAGTT CAAGCCCAAC AGTGTCACGT ACAGGACCAA ATCCGGCAAC 
ATCTTGCTGC ACAAGTCGAC CATTAACAGG GAGGCCACCC GGCTGCGGAG CCGATGGCTG 
ACGTATTTCA TGGCTGTCAA GTCCAACGGC AGCGTCTTCG TCCGGGACTC CTCCCAGGTG 
CACCCACTAG CTGTGCTGCT ACTGACCGAC GGGGACGTCC ACATCCGTGA TGACGGGCGC 
CGGGCCACCA TTTCCCTGAG CGACAGTGAC CTGCTGCGGC TGGAGGGTGA CTCGCGCACC 
GTGCGGCTGC TGCGGGAGCT GCGCCGGGCC CTGGGCCGCA TGGTGGAGCG GAGCCTGCGC 
AGCGAGCTGG CGGCACTGCC GCCCTGTGTG CAGGAGGAGC ATGGGCAGCT GCTCGCCCTG 
CTGGCGGAGC TGCTGCGCGG GCCCTGCGGC AGCTTTGATG TGCGCAAGAC CGCTGACGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025128429.1
CDS	566..4228
Translation	MATARRLMALAAGVSPRLRPSGPRAIGRQGGPRGLSTGCSRPDRTQEAAEAEAASGEPGEGDGSVVNASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGSLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHAPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWISSELRLQGEDILPLGKDSGPLSDPITGKPYVPLSEAEELRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVIAGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVVVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPIGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMAPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVSGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLRELRRALGRMVERSLRSELAALPPCVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_025272644.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025272644.1

ORF Insert Sequence:

ATGGCGACCG CCAGGAGACT CATGGCGCTG GCCGCCGGCG TCTCTCCGCG CCTGCGGCCA 
TCGGGTCCCC GCGCCATCGG GCGACAGGGA GGCCCGCGCG GCCTCTCCAC AGGCTGCTCC 
CGCCCCGACC GCACCCAGGA GGCCGCCGAG GCCGAGGCGG CCTCCGGCGA GCCTGGGGAA 
GGCGATGGAA GCGTGGTGAA CGCTTCTAGG GACCTATTAA AAGAGTTTCC ACAGCCCAAA 
AATCTTCTCA ACAGTGTGAT TGGAAGAGCA CTTGGCATCT CACACGCAAA AGACAAATTG 
GTCTACGTGC ATACAAATGG ACCGAAGAAA AAGAAAGTCA CCCTCCACAT AAAGTGGCCC 
AAGAGCGTGG AGGTAGAAGG CTATGGCAGC AAGAAGATCG ATGCCGAGAG GCAGGCTGCA 
GCTGCGGCCT GCCAGCTGTT CAAGGGCTGG GGTCTGCTGG GTCCCCGGAA TGAGCTGTTT 
GATGCAGCCA AATACCGCGT GCTAGCCGAT CGCTTTGGCT CTCCGGCTGA CAGCTGGTGG 
CGCCCAGAAC CCACCATGCC ACCCACTTCC TGGCGGCAGC TGAATCCTGA GAGCATCCGG 
CCAGGGGGAC CTGGGAGCCT GTCCCGCTCC TTGGGTCGGG AGGAAGAGGA GGATGAGGAG 
GAAGAACTAG AAGAAGGGAC CATCGATGTC ACTGAATTTC TGTCTATGAC CCAGCAGGAC 
TCCCACGCCC CACTCAGGGA TTCCAGGGGG GGTTCCTTTG AAATGACAGA TGACGACAGT 
GCTATCAGGG CTCTGACCCA GTTTCCACTT CCCAAGAACC TTCTGGCCAA GGTGATTCAG 
ATAGCAACAT CCTCCTCCAC AGCCAAGAAC CTCATGCAGT TCCACACCGT GGGCACCAAG 
ACCAAGCTGT CCACCCTCAC CCTGCTCTGG CCCTGTCCCA TGACCTTCGT GGCCAAGGGG 
CGTCGCAAAG CAGAGGCAGA GAATAAAGCG GCAGCCTTGG CTTGTAAGAA ACTGAAGAGC 
CTGGGACTGG TGGACCGGAA CAACGAGCCG CTCACCCACG CCATGTATAA CTTGGCCTCC 
TTGCGAGAGC TGGGTGAGAC CCAGCGCCGG CCGTGTACCA TCCAGGTGCC TGAGCCCATC 
CTCCGCAAGA TCGAGACCTT CCTTAACCAT TACCCTGTGG AGAGTTCGTG GATCTCCTCA 
GAGCTCCGGC TGCAGGGTGA GGACATCCTG CCCTTGGGCA AGGACTCGGG GCCCCTGAGT 
GACCCCATCA CAGGCAAGCC CTATGTGCCA CTGTCAGAAG CAGAGGAGCT GCGTCTGAGC 
CAGAGTCTGC TGGAGCTGTG GCGGCGGCGA GGGCCAGTCT GGCAGGAGGC ACCCCAGCTC 
CCCGTGGACC CTCATCGGGA CACCATCCTC AATGCCATCG AGCAGCACCC AGTGGTGGTC 
ATTGCTGGGG ACACGGGCTG TGGGAAGACC ACGCGCATCC CCCAGCTGCT GCTGGAGCGC 
TACGTGACCG AGGGCCGCGG TGCGCGCTGC AACGTGATCA TCACCCAGCC ACGCCGCATC 
TCGGCCGTGT CTGTGGCGCA GCGGGTCAGC CACGAACTGG GCCCTTCCCT GCGCCGGAAC 
GTAGGCTTCC AGGTGCGGTT GGAAAGCAAG CCTCCGGCCC GAGGAGGGGC CCTGCTGTTC 
TGCACGGTGG GCATCCTGCT GCGGAAGCTG CAGAGCAACC CCAGCCTGGA GGGCGTGAGC 
CACGTGGTCG TGGACGAGGT GCACGAGCGA GACGTGAACA CGGACTTCCT GCTGATCCTG 
CTCAAGGGCC TGCAGCGGCT CAACCCGGCT CTGCGCTTGG TGCTCATGAG CGCCACTGGC 
GACAACGAGC GTTTCTCCCG CTACTTTGGT GGCTGCCCTG TCATCAAGGT CCCCGGCTTC 
ATGTACCCTG TCAAGGAGCA CTACCTGGAG GACATCCTGG CCAAGCTGGG CAAGCACCAG 
TATCCGCACC GGCACCGGCA CCACGAGTCT GAGGATGAAT GCGCTCTCGA TTTGGACCTC 
GTGACTGATC TGGTTCTGCA CATCGATGCC CGCGGGGAAC CAGGTGGGAT CCTCTGCTTC 
CTGCCGGGCT GGCAGGAGAT CAAAGGAGTC CAGCAACGCC TCCAGGAGGC CCTGGGCATG 
CACGAGAGCA AGTACCTCAT CCTGCCAGTG CACTCCAACA TCCCGATGAT GGACCAGAAG 
GCCATATTCC AGCAGCCTCC CATTGGGGTA CGCAAGATCG TCTTGGCCAC CAACATTGCC 
GAGACGTCCA TCACGATCAA TGACATCGTG CACGTGGTGG ACAGCGGTCT GCACAAGGAG 
GAGCGCTACG ACCTGAAGAC CAAGGTTTCC TGCCTGGAGA CCGTGTGGGT GTCACGAGCC 
AATGTGATCC AGCGCCGGGG CCGGGCGGGC CGCTGCCAGT CTGGCTTTGC CTACCACCTC 
TTCCCACGGA GCCGGCTGGA GAAGATGGCT CCTTTCCAAG TGCCAGAGAT CCTGCGCACA 
CCCCTGGAGA ACCTGGTGCT GCAGGCCAAG ATCCACATGC CAGAGAAGAC GGCAGTGGAG 
TTCCTCTCCA AGGCCGTAGA CAGTCCAAAC ATCAAGGCGG TGGACGAGGC CGTGATCTTG 
CTCCAAGAGA TTGGGGTTCT GGACCAGCGG GAGTACCTGA CCACCCTGGG GCAGCGCCTG 
GCCCACATCT CCACTGACCC GCGGCTGGCC AAGGCCATCG TGCTGGCTGC CATCTTCCGT 
TGCCTGCACC CGCTGTTAGT GGTCGTCTCG TGCCTCACCC GGGACCCCTT CAGCAGCAGC 
CTGCAGAACC GGGCGGAGGT GGACAAGGTG AAAGCACTGT TGAGCCACGA CAGCGGCAGT 
GACCACCTGG CCTTTGTGCG GGCGGTGTCC GGCTGGGAGG AGGTGCTGCG CTGGCAGGAT 
CGCAGCTCCC GTGAGAACTA CCTGGAGGAA AACCTGCTCT ACGCACCCAG CCTGCGCTTC 
ATCCACGGAC TCATCAAGCA GTTCTCAGAG AACATTTATG AGGCTTTCCT GGTTGGGAAG 
CCCTCGGACT GCACCCTGGC CTCTGCCCAG TGCAACGAGT ACAGTGAGGA GGAGGAGCTG 
GTGAAGGGGG TGCTGATGGC GGGTCTCTAC CCCAACCTCA TCCAGGTGAG GCAGGGCAAG 
GTGACCCGGC AGGGCAAGTT CAAGCCCAAC AGTGTCACGT ACAGGACCAA ATCCGGCAAC 
ATCTTGCTGC ACAAGTCGAC CATTAACAGG GAGGCCACCC GGCTGCGGAG CCGATGGCTG 
ACGTATTTCA TGGCTGTCAA GTCCAACGGC AGCGTCTTCG TCCGGGACTC CTCCCAGGTG 
CACCCACTAG CTGTGCTGCT ACTGACCGAC GGGGACGTCC ACATCCGTGA TGACGGGCGC 
CGGGCCACCA TTTCCCTGAG CGACAGTGAC CTGCTGCGGC TGGAGGGTGA CTCGCGCACC 
GTGCGGCTGC TGCGGGAGCT GCGCCGGGCC CTGGGCCGCA TGGTGGAGCG GAGCCTGCGC 
AGCGAGCTGG CGGCACTGCC GCCCTGTGTG CAGGAGGAGC ATGGGCAGCT GCTCGCCCTG 
CTGGCGGAGC TGCTGCGCGG GCCCTGCGGC AGCTTTGATG TGCGCAAGAC CGCTGACGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_025272646.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025128431.1 Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X4, mRNA.

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CloneID	OBu57930
Clone ID Related Accession (Same CDS sequence)	XM_025272646.1 , XM_025272645.1
Accession Version	XM_025272646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	putative ATP-dependent RNA helicase DHX30 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCC TGGACTCATT CAGGAAAGAT CGGGCCCAAC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CACTTGGCAT CTCACACGCA AAAGACAAAT TGGTCTACGT GCATACAAAT 
GGACCGAAGA AAAAGAAAGT CACCCTCCAC ATAAAGTGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCCGAG AGGCAGGCTG CAGCTGCGGC CTGCCAGCTG 
TTCAAGGGCT GGGGTCTGCT GGGTCCCCGG AATGAGCTGT TTGATGCAGC CAAATACCGC 
GTGCTAGCCG ATCGCTTTGG CTCTCCGGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCACCCACTT CCTGGCGGCA GCTGAATCCT GAGAGCATCC GGCCAGGGGG ACCTGGGAGC 
CTGTCCCGCT CCTTGGGTCG GGAGGAAGAG GAGGATGAGG AGGAAGAACT AGAAGAAGGG 
ACCATCGATG TCACTGAATT TCTGTCTATG ACCCAGCAGG ACTCCCACGC CCCACTCAGG 
GATTCCAGGG GGGGTTCCTT TGAAATGACA GATGACGACA GTGCTATCAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATAGCAAC ATCCTCCTCC 
ACAGCCAAGA ACCTCATGCA GTTCCACACC GTGGGCACCA AGACCAAGCT GTCCACCCTC 
ACCCTGCTCT GGCCCTGTCC CATGACCTTC GTGGCCAAGG GGCGTCGCAA AGCAGAGGCA 
GAGAATAAAG CGGCAGCCTT GGCTTGTAAG AAACTGAAGA GCCTGGGACT GGTGGACCGG 
AACAACGAGC CGCTCACCCA CGCCATGTAT AACTTGGCCT CCTTGCGAGA GCTGGGTGAG 
ACCCAGCGCC GGCCGTGTAC CATCCAGGTG CCTGAGCCCA TCCTCCGCAA GATCGAGACC 
TTCCTTAACC ATTACCCTGT GGAGAGTTCG TGGATCTCCT CAGAGCTCCG GCTGCAGGGT 
GAGGACATCC TGCCCTTGGG CAAGGACTCG GGGCCCCTGA GTGACCCCAT CACAGGCAAG 
CCCTATGTGC CACTGTCAGA AGCAGAGGAG CTGCGTCTGA GCCAGAGTCT GCTGGAGCTG 
TGGCGGCGGC GAGGGCCAGT CTGGCAGGAG GCACCCCAGC TCCCCGTGGA CCCTCATCGG 
GACACCATCC TCAATGCCAT CGAGCAGCAC CCAGTGGTGG TCATTGCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG CTGCTGGAGC GCTACGTGAC CGAGGGCCGC 
GGTGCGCGCT GCAACGTGAT CATCACCCAG CCACGCCGCA TCTCGGCCGT GTCTGTGGCG 
CAGCGGGTCA GCCACGAACT GGGCCCTTCC CTGCGCCGGA ACGTAGGCTT CCAGGTGCGG 
TTGGAAAGCA AGCCTCCGGC CCGAGGAGGG GCCCTGCTGT TCTGCACGGT GGGCATCCTG 
CTGCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTGGT CGTGGACGAG 
GTGCACGAGC GAGACGTGAA CACGGACTTC CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CTCTGCGCTT GGTGCTCATG AGCGCCACTG GCGACAACGA GCGTTTCTCC 
CGCTACTTTG GTGGCTGCCC TGTCATCAAG GTCCCCGGCT TCATGTACCC TGTCAAGGAG 
CACTACCTGG AGGACATCCT GGCCAAGCTG GGCAAGCACC AGTATCCGCA CCGGCACCGG 
CACCACGAGT CTGAGGATGA ATGCGCTCTC GATTTGGACC TCGTGACTGA TCTGGTTCTG 
CACATCGATG CCCGCGGGGA ACCAGGTGGG ATCCTCTGCT TCCTGCCGGG CTGGCAGGAG 
ATCAAAGGAG TCCAGCAACG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCGATG ATGGACCAGA AGGCCATATT CCAGCAGCCT 
CCCATTGGGG TACGCAAGAT CGTCTTGGCC ACCAACATTG CCGAGACGTC CATCACGATC 
AATGACATCG TGCACGTGGT GGACAGCGGT CTGCACAAGG AGGAGCGCTA CGACCTGAAG 
ACCAAGGTTT CCTGCCTGGA GACCGTGTGG GTGTCACGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCTGGCTTT GCCTACCACC TCTTCCCACG GAGCCGGCTG 
GAGAAGATGG CTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCCCTGGA GAACCTGGTG 
CTGCAGGCCA AGATCCACAT GCCAGAGAAG ACGGCAGTGG AGTTCCTCTC CAAGGCCGTA 
GACAGTCCAA ACATCAAGGC GGTGGACGAG GCCGTGATCT TGCTCCAAGA GATTGGGGTT 
CTGGACCAGC GGGAGTACCT GACCACCCTG GGGCAGCGCC TGGCCCACAT CTCCACTGAC 
CCGCGGCTGG CCAAGGCCAT CGTGCTGGCT GCCATCTTCC GTTGCCTGCA CCCGCTGTTA 
GTGGTCGTCT CGTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTGCAGAA CCGGGCGGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAC GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCGGTGT CCGGCTGGGA GGAGGTGCTG CGCTGGCAGG ATCGCAGCTC CCGTGAGAAC 
TACCTGGAGG AAAACCTGCT CTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTGGTTGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCTGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG GGTGCTGATG 
GCGGGTCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTGACCCG GCAGGGCAAG 
TTCAAGCCCA ACAGTGTCAC GTACAGGACC AAATCCGGCA ACATCTTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC CCGGCTGCGG AGCCGATGGC TGACGTATTT CATGGCTGTC 
AAGTCCAACG GCAGCGTCTT CGTCCGGGAC TCCTCCCAGG TGCACCCACT AGCTGTGCTG 
CTACTGACCG ACGGGGACGT CCACATCCGT GATGACGGGC GCCGGGCCAC CATTTCCCTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGCA CCGTGCGGCT GCTGCGGGAG 
CTGCGCCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCGGCACTG 
CCGCCCTGTG TGCAGGAGGA GCATGGGCAG CTGCTCGCCC TGCTGGCGGA GCTGCTGCGC 
GGGCCCTGCG GCAGCTTTGA TGTGCGCAAG ACCGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025128431.1
CDS	259..3843
Translation	MFSLDSFRKDRAQHRQRQCKLPPPRLPPMCVNPAPGGTISRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGSLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHAPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWISSELRLQGEDILPLGKDSGPLSDPITGKPYVPLSEAEELRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVIAGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVVVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPIGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMAPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVSGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLRELRRALGRMVERSLRSELAALPPCVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_025272646.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025272646.1

ORF Insert Sequence:

ATGTTCAGCC TGGACTCATT CAGGAAAGAT CGGGCCCAAC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CACTTGGCAT CTCACACGCA AAAGACAAAT TGGTCTACGT GCATACAAAT 
GGACCGAAGA AAAAGAAAGT CACCCTCCAC ATAAAGTGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCCGAG AGGCAGGCTG CAGCTGCGGC CTGCCAGCTG 
TTCAAGGGCT GGGGTCTGCT GGGTCCCCGG AATGAGCTGT TTGATGCAGC CAAATACCGC 
GTGCTAGCCG ATCGCTTTGG CTCTCCGGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCACCCACTT CCTGGCGGCA GCTGAATCCT GAGAGCATCC GGCCAGGGGG ACCTGGGAGC 
CTGTCCCGCT CCTTGGGTCG GGAGGAAGAG GAGGATGAGG AGGAAGAACT AGAAGAAGGG 
ACCATCGATG TCACTGAATT TCTGTCTATG ACCCAGCAGG ACTCCCACGC CCCACTCAGG 
GATTCCAGGG GGGGTTCCTT TGAAATGACA GATGACGACA GTGCTATCAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATAGCAAC ATCCTCCTCC 
ACAGCCAAGA ACCTCATGCA GTTCCACACC GTGGGCACCA AGACCAAGCT GTCCACCCTC 
ACCCTGCTCT GGCCCTGTCC CATGACCTTC GTGGCCAAGG GGCGTCGCAA AGCAGAGGCA 
GAGAATAAAG CGGCAGCCTT GGCTTGTAAG AAACTGAAGA GCCTGGGACT GGTGGACCGG 
AACAACGAGC CGCTCACCCA CGCCATGTAT AACTTGGCCT CCTTGCGAGA GCTGGGTGAG 
ACCCAGCGCC GGCCGTGTAC CATCCAGGTG CCTGAGCCCA TCCTCCGCAA GATCGAGACC 
TTCCTTAACC ATTACCCTGT GGAGAGTTCG TGGATCTCCT CAGAGCTCCG GCTGCAGGGT 
GAGGACATCC TGCCCTTGGG CAAGGACTCG GGGCCCCTGA GTGACCCCAT CACAGGCAAG 
CCCTATGTGC CACTGTCAGA AGCAGAGGAG CTGCGTCTGA GCCAGAGTCT GCTGGAGCTG 
TGGCGGCGGC GAGGGCCAGT CTGGCAGGAG GCACCCCAGC TCCCCGTGGA CCCTCATCGG 
GACACCATCC TCAATGCCAT CGAGCAGCAC CCAGTGGTGG TCATTGCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG CTGCTGGAGC GCTACGTGAC CGAGGGCCGC 
GGTGCGCGCT GCAACGTGAT CATCACCCAG CCACGCCGCA TCTCGGCCGT GTCTGTGGCG 
CAGCGGGTCA GCCACGAACT GGGCCCTTCC CTGCGCCGGA ACGTAGGCTT CCAGGTGCGG 
TTGGAAAGCA AGCCTCCGGC CCGAGGAGGG GCCCTGCTGT TCTGCACGGT GGGCATCCTG 
CTGCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTGGT CGTGGACGAG 
GTGCACGAGC GAGACGTGAA CACGGACTTC CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CTCTGCGCTT GGTGCTCATG AGCGCCACTG GCGACAACGA GCGTTTCTCC 
CGCTACTTTG GTGGCTGCCC TGTCATCAAG GTCCCCGGCT TCATGTACCC TGTCAAGGAG 
CACTACCTGG AGGACATCCT GGCCAAGCTG GGCAAGCACC AGTATCCGCA CCGGCACCGG 
CACCACGAGT CTGAGGATGA ATGCGCTCTC GATTTGGACC TCGTGACTGA TCTGGTTCTG 
CACATCGATG CCCGCGGGGA ACCAGGTGGG ATCCTCTGCT TCCTGCCGGG CTGGCAGGAG 
ATCAAAGGAG TCCAGCAACG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCGATG ATGGACCAGA AGGCCATATT CCAGCAGCCT 
CCCATTGGGG TACGCAAGAT CGTCTTGGCC ACCAACATTG CCGAGACGTC CATCACGATC 
AATGACATCG TGCACGTGGT GGACAGCGGT CTGCACAAGG AGGAGCGCTA CGACCTGAAG 
ACCAAGGTTT CCTGCCTGGA GACCGTGTGG GTGTCACGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCTGGCTTT GCCTACCACC TCTTCCCACG GAGCCGGCTG 
GAGAAGATGG CTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCCCTGGA GAACCTGGTG 
CTGCAGGCCA AGATCCACAT GCCAGAGAAG ACGGCAGTGG AGTTCCTCTC CAAGGCCGTA 
GACAGTCCAA ACATCAAGGC GGTGGACGAG GCCGTGATCT TGCTCCAAGA GATTGGGGTT 
CTGGACCAGC GGGAGTACCT GACCACCCTG GGGCAGCGCC TGGCCCACAT CTCCACTGAC 
CCGCGGCTGG CCAAGGCCAT CGTGCTGGCT GCCATCTTCC GTTGCCTGCA CCCGCTGTTA 
GTGGTCGTCT CGTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTGCAGAA CCGGGCGGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAC GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCGGTGT CCGGCTGGGA GGAGGTGCTG CGCTGGCAGG ATCGCAGCTC CCGTGAGAAC 
TACCTGGAGG AAAACCTGCT CTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTGGTTGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCTGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG GGTGCTGATG 
GCGGGTCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTGACCCG GCAGGGCAAG 
TTCAAGCCCA ACAGTGTCAC GTACAGGACC AAATCCGGCA ACATCTTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC CCGGCTGCGG AGCCGATGGC TGACGTATTT CATGGCTGTC 
AAGTCCAACG GCAGCGTCTT CGTCCGGGAC TCCTCCCAGG TGCACCCACT AGCTGTGCTG 
CTACTGACCG ACGGGGACGT CCACATCCGT GATGACGGGC GCCGGGCCAC CATTTCCCTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGCA CCGTGCGGCT GCTGCGGGAG 
CTGCGCCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCGGCACTG 
CCGCCCTGTG TGCAGGAGGA GCATGGGCAG CTGCTCGCCC TGCTGGCGGA GCTGCTGCGC 
GGGCCCTGCG GCAGCTTTGA TGTGCGCAAG ACCGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_025272645.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025128430.1 Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X3, mRNA.

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CloneID	OBu57930
Clone ID Related Accession (Same CDS sequence)	XM_025272646.1 , XM_025272645.1
Accession Version	XM_025272645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	putative ATP-dependent RNA helicase DHX30 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCC TGGACTCATT CAGGAAAGAT CGGGCCCAAC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CACTTGGCAT CTCACACGCA AAAGACAAAT TGGTCTACGT GCATACAAAT 
GGACCGAAGA AAAAGAAAGT CACCCTCCAC ATAAAGTGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCCGAG AGGCAGGCTG CAGCTGCGGC CTGCCAGCTG 
TTCAAGGGCT GGGGTCTGCT GGGTCCCCGG AATGAGCTGT TTGATGCAGC CAAATACCGC 
GTGCTAGCCG ATCGCTTTGG CTCTCCGGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCACCCACTT CCTGGCGGCA GCTGAATCCT GAGAGCATCC GGCCAGGGGG ACCTGGGAGC 
CTGTCCCGCT CCTTGGGTCG GGAGGAAGAG GAGGATGAGG AGGAAGAACT AGAAGAAGGG 
ACCATCGATG TCACTGAATT TCTGTCTATG ACCCAGCAGG ACTCCCACGC CCCACTCAGG 
GATTCCAGGG GGGGTTCCTT TGAAATGACA GATGACGACA GTGCTATCAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATAGCAAC ATCCTCCTCC 
ACAGCCAAGA ACCTCATGCA GTTCCACACC GTGGGCACCA AGACCAAGCT GTCCACCCTC 
ACCCTGCTCT GGCCCTGTCC CATGACCTTC GTGGCCAAGG GGCGTCGCAA AGCAGAGGCA 
GAGAATAAAG CGGCAGCCTT GGCTTGTAAG AAACTGAAGA GCCTGGGACT GGTGGACCGG 
AACAACGAGC CGCTCACCCA CGCCATGTAT AACTTGGCCT CCTTGCGAGA GCTGGGTGAG 
ACCCAGCGCC GGCCGTGTAC CATCCAGGTG CCTGAGCCCA TCCTCCGCAA GATCGAGACC 
TTCCTTAACC ATTACCCTGT GGAGAGTTCG TGGATCTCCT CAGAGCTCCG GCTGCAGGGT 
GAGGACATCC TGCCCTTGGG CAAGGACTCG GGGCCCCTGA GTGACCCCAT CACAGGCAAG 
CCCTATGTGC CACTGTCAGA AGCAGAGGAG CTGCGTCTGA GCCAGAGTCT GCTGGAGCTG 
TGGCGGCGGC GAGGGCCAGT CTGGCAGGAG GCACCCCAGC TCCCCGTGGA CCCTCATCGG 
GACACCATCC TCAATGCCAT CGAGCAGCAC CCAGTGGTGG TCATTGCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG CTGCTGGAGC GCTACGTGAC CGAGGGCCGC 
GGTGCGCGCT GCAACGTGAT CATCACCCAG CCACGCCGCA TCTCGGCCGT GTCTGTGGCG 
CAGCGGGTCA GCCACGAACT GGGCCCTTCC CTGCGCCGGA ACGTAGGCTT CCAGGTGCGG 
TTGGAAAGCA AGCCTCCGGC CCGAGGAGGG GCCCTGCTGT TCTGCACGGT GGGCATCCTG 
CTGCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTGGT CGTGGACGAG 
GTGCACGAGC GAGACGTGAA CACGGACTTC CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CTCTGCGCTT GGTGCTCATG AGCGCCACTG GCGACAACGA GCGTTTCTCC 
CGCTACTTTG GTGGCTGCCC TGTCATCAAG GTCCCCGGCT TCATGTACCC TGTCAAGGAG 
CACTACCTGG AGGACATCCT GGCCAAGCTG GGCAAGCACC AGTATCCGCA CCGGCACCGG 
CACCACGAGT CTGAGGATGA ATGCGCTCTC GATTTGGACC TCGTGACTGA TCTGGTTCTG 
CACATCGATG CCCGCGGGGA ACCAGGTGGG ATCCTCTGCT TCCTGCCGGG CTGGCAGGAG 
ATCAAAGGAG TCCAGCAACG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCGATG ATGGACCAGA AGGCCATATT CCAGCAGCCT 
CCCATTGGGG TACGCAAGAT CGTCTTGGCC ACCAACATTG CCGAGACGTC CATCACGATC 
AATGACATCG TGCACGTGGT GGACAGCGGT CTGCACAAGG AGGAGCGCTA CGACCTGAAG 
ACCAAGGTTT CCTGCCTGGA GACCGTGTGG GTGTCACGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCTGGCTTT GCCTACCACC TCTTCCCACG GAGCCGGCTG 
GAGAAGATGG CTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCCCTGGA GAACCTGGTG 
CTGCAGGCCA AGATCCACAT GCCAGAGAAG ACGGCAGTGG AGTTCCTCTC CAAGGCCGTA 
GACAGTCCAA ACATCAAGGC GGTGGACGAG GCCGTGATCT TGCTCCAAGA GATTGGGGTT 
CTGGACCAGC GGGAGTACCT GACCACCCTG GGGCAGCGCC TGGCCCACAT CTCCACTGAC 
CCGCGGCTGG CCAAGGCCAT CGTGCTGGCT GCCATCTTCC GTTGCCTGCA CCCGCTGTTA 
GTGGTCGTCT CGTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTGCAGAA CCGGGCGGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAC GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCGGTGT CCGGCTGGGA GGAGGTGCTG CGCTGGCAGG ATCGCAGCTC CCGTGAGAAC 
TACCTGGAGG AAAACCTGCT CTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTGGTTGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCTGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG GGTGCTGATG 
GCGGGTCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTGACCCG GCAGGGCAAG 
TTCAAGCCCA ACAGTGTCAC GTACAGGACC AAATCCGGCA ACATCTTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC CCGGCTGCGG AGCCGATGGC TGACGTATTT CATGGCTGTC 
AAGTCCAACG GCAGCGTCTT CGTCCGGGAC TCCTCCCAGG TGCACCCACT AGCTGTGCTG 
CTACTGACCG ACGGGGACGT CCACATCCGT GATGACGGGC GCCGGGCCAC CATTTCCCTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGCA CCGTGCGGCT GCTGCGGGAG 
CTGCGCCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCGGCACTG 
CCGCCCTGTG TGCAGGAGGA GCATGGGCAG CTGCTCGCCC TGCTGGCGGA GCTGCTGCGC 
GGGCCCTGCG GCAGCTTTGA TGTGCGCAAG ACCGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025128430.1
CDS	165..3749
Translation	MFSLDSFRKDRAQHRQRQCKLPPPRLPPMCVNPAPGGTISRASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGSLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHAPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWISSELRLQGEDILPLGKDSGPLSDPITGKPYVPLSEAEELRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVIAGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVVVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPIGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMAPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVSGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLRELRRALGRMVERSLRSELAALPPCVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_025272645.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025272645.1

ORF Insert Sequence:

ATGTTCAGCC TGGACTCATT CAGGAAAGAT CGGGCCCAAC ACAGGCAGCG TCAGTGCAAA 
CTTCCCCCAC CCCGTCTTCC ACCCATGTGT GTCAACCCTG CCCCTGGAGG GACCATCTCT 
CGAGCTTCTA GGGACCTATT AAAAGAGTTT CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CACTTGGCAT CTCACACGCA AAAGACAAAT TGGTCTACGT GCATACAAAT 
GGACCGAAGA AAAAGAAAGT CACCCTCCAC ATAAAGTGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCCGAG AGGCAGGCTG CAGCTGCGGC CTGCCAGCTG 
TTCAAGGGCT GGGGTCTGCT GGGTCCCCGG AATGAGCTGT TTGATGCAGC CAAATACCGC 
GTGCTAGCCG ATCGCTTTGG CTCTCCGGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCACCCACTT CCTGGCGGCA GCTGAATCCT GAGAGCATCC GGCCAGGGGG ACCTGGGAGC 
CTGTCCCGCT CCTTGGGTCG GGAGGAAGAG GAGGATGAGG AGGAAGAACT AGAAGAAGGG 
ACCATCGATG TCACTGAATT TCTGTCTATG ACCCAGCAGG ACTCCCACGC CCCACTCAGG 
GATTCCAGGG GGGGTTCCTT TGAAATGACA GATGACGACA GTGCTATCAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATAGCAAC ATCCTCCTCC 
ACAGCCAAGA ACCTCATGCA GTTCCACACC GTGGGCACCA AGACCAAGCT GTCCACCCTC 
ACCCTGCTCT GGCCCTGTCC CATGACCTTC GTGGCCAAGG GGCGTCGCAA AGCAGAGGCA 
GAGAATAAAG CGGCAGCCTT GGCTTGTAAG AAACTGAAGA GCCTGGGACT GGTGGACCGG 
AACAACGAGC CGCTCACCCA CGCCATGTAT AACTTGGCCT CCTTGCGAGA GCTGGGTGAG 
ACCCAGCGCC GGCCGTGTAC CATCCAGGTG CCTGAGCCCA TCCTCCGCAA GATCGAGACC 
TTCCTTAACC ATTACCCTGT GGAGAGTTCG TGGATCTCCT CAGAGCTCCG GCTGCAGGGT 
GAGGACATCC TGCCCTTGGG CAAGGACTCG GGGCCCCTGA GTGACCCCAT CACAGGCAAG 
CCCTATGTGC CACTGTCAGA AGCAGAGGAG CTGCGTCTGA GCCAGAGTCT GCTGGAGCTG 
TGGCGGCGGC GAGGGCCAGT CTGGCAGGAG GCACCCCAGC TCCCCGTGGA CCCTCATCGG 
GACACCATCC TCAATGCCAT CGAGCAGCAC CCAGTGGTGG TCATTGCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG CTGCTGGAGC GCTACGTGAC CGAGGGCCGC 
GGTGCGCGCT GCAACGTGAT CATCACCCAG CCACGCCGCA TCTCGGCCGT GTCTGTGGCG 
CAGCGGGTCA GCCACGAACT GGGCCCTTCC CTGCGCCGGA ACGTAGGCTT CCAGGTGCGG 
TTGGAAAGCA AGCCTCCGGC CCGAGGAGGG GCCCTGCTGT TCTGCACGGT GGGCATCCTG 
CTGCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTGGT CGTGGACGAG 
GTGCACGAGC GAGACGTGAA CACGGACTTC CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CTCTGCGCTT GGTGCTCATG AGCGCCACTG GCGACAACGA GCGTTTCTCC 
CGCTACTTTG GTGGCTGCCC TGTCATCAAG GTCCCCGGCT TCATGTACCC TGTCAAGGAG 
CACTACCTGG AGGACATCCT GGCCAAGCTG GGCAAGCACC AGTATCCGCA CCGGCACCGG 
CACCACGAGT CTGAGGATGA ATGCGCTCTC GATTTGGACC TCGTGACTGA TCTGGTTCTG 
CACATCGATG CCCGCGGGGA ACCAGGTGGG ATCCTCTGCT TCCTGCCGGG CTGGCAGGAG 
ATCAAAGGAG TCCAGCAACG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCGATG ATGGACCAGA AGGCCATATT CCAGCAGCCT 
CCCATTGGGG TACGCAAGAT CGTCTTGGCC ACCAACATTG CCGAGACGTC CATCACGATC 
AATGACATCG TGCACGTGGT GGACAGCGGT CTGCACAAGG AGGAGCGCTA CGACCTGAAG 
ACCAAGGTTT CCTGCCTGGA GACCGTGTGG GTGTCACGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCTGGCTTT GCCTACCACC TCTTCCCACG GAGCCGGCTG 
GAGAAGATGG CTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCCCTGGA GAACCTGGTG 
CTGCAGGCCA AGATCCACAT GCCAGAGAAG ACGGCAGTGG AGTTCCTCTC CAAGGCCGTA 
GACAGTCCAA ACATCAAGGC GGTGGACGAG GCCGTGATCT TGCTCCAAGA GATTGGGGTT 
CTGGACCAGC GGGAGTACCT GACCACCCTG GGGCAGCGCC TGGCCCACAT CTCCACTGAC 
CCGCGGCTGG CCAAGGCCAT CGTGCTGGCT GCCATCTTCC GTTGCCTGCA CCCGCTGTTA 
GTGGTCGTCT CGTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTGCAGAA CCGGGCGGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAC GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCGGTGT CCGGCTGGGA GGAGGTGCTG CGCTGGCAGG ATCGCAGCTC CCGTGAGAAC 
TACCTGGAGG AAAACCTGCT CTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTGGTTGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCTGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG GGTGCTGATG 
GCGGGTCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTGACCCG GCAGGGCAAG 
TTCAAGCCCA ACAGTGTCAC GTACAGGACC AAATCCGGCA ACATCTTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC CCGGCTGCGG AGCCGATGGC TGACGTATTT CATGGCTGTC 
AAGTCCAACG GCAGCGTCTT CGTCCGGGAC TCCTCCCAGG TGCACCCACT AGCTGTGCTG 
CTACTGACCG ACGGGGACGT CCACATCCGT GATGACGGGC GCCGGGCCAC CATTTCCCTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGCA CCGTGCGGCT GCTGCGGGAG 
CTGCGCCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCGGCACTG 
CCGCCCTGTG TGCAGGAGGA GCATGGGCAG CTGCTCGCCC TGCTGGCGGA GCTGCTGCGC 
GGGCCCTGCG GCAGCTTTGA TGTGCGCAAG ACCGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_025272647.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025128432.1 Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X5, mRNA.

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CloneID	OBu57931
Clone ID Related Accession (Same CDS sequence)	XM_025272647.1 , XM_025272648.1
Accession Version	XM_025272647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	putative ATP-dependent RNA helicase DHX30 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCC TGGACTCATT CAGGAAAGCT TCTAGGGACC TATTAAAAGA GTTTCCACAG 
CCCAAAAATC TTCTCAACAG TGTGATTGGA AGAGCACTTG GCATCTCACA CGCAAAAGAC 
AAATTGGTCT ACGTGCATAC AAATGGACCG AAGAAAAAGA AAGTCACCCT CCACATAAAG 
TGGCCCAAGA GCGTGGAGGT AGAAGGCTAT GGCAGCAAGA AGATCGATGC CGAGAGGCAG 
GCTGCAGCTG CGGCCTGCCA GCTGTTCAAG GGCTGGGGTC TGCTGGGTCC CCGGAATGAG 
CTGTTTGATG CAGCCAAATA CCGCGTGCTA GCCGATCGCT TTGGCTCTCC GGCTGACAGC 
TGGTGGCGCC CAGAACCCAC CATGCCACCC ACTTCCTGGC GGCAGCTGAA TCCTGAGAGC 
ATCCGGCCAG GGGGACCTGG GAGCCTGTCC CGCTCCTTGG GTCGGGAGGA AGAGGAGGAT 
GAGGAGGAAG AACTAGAAGA AGGGACCATC GATGTCACTG AATTTCTGTC TATGACCCAG 
CAGGACTCCC ACGCCCCACT CAGGGATTCC AGGGGGGGTT CCTTTGAAAT GACAGATGAC 
GACAGTGCTA TCAGGGCTCT GACCCAGTTT CCACTTCCCA AGAACCTTCT GGCCAAGGTG 
ATTCAGATAG CAACATCCTC CTCCACAGCC AAGAACCTCA TGCAGTTCCA CACCGTGGGC 
ACCAAGACCA AGCTGTCCAC CCTCACCCTG CTCTGGCCCT GTCCCATGAC CTTCGTGGCC 
AAGGGGCGTC GCAAAGCAGA GGCAGAGAAT AAAGCGGCAG CCTTGGCTTG TAAGAAACTG 
AAGAGCCTGG GACTGGTGGA CCGGAACAAC GAGCCGCTCA CCCACGCCAT GTATAACTTG 
GCCTCCTTGC GAGAGCTGGG TGAGACCCAG CGCCGGCCGT GTACCATCCA GGTGCCTGAG 
CCCATCCTCC GCAAGATCGA GACCTTCCTT AACCATTACC CTGTGGAGAG TTCGTGGATC 
TCCTCAGAGC TCCGGCTGCA GGGTGAGGAC ATCCTGCCCT TGGGCAAGGA CTCGGGGCCC 
CTGAGTGACC CCATCACAGG CAAGCCCTAT GTGCCACTGT CAGAAGCAGA GGAGCTGCGT 
CTGAGCCAGA GTCTGCTGGA GCTGTGGCGG CGGCGAGGGC CAGTCTGGCA GGAGGCACCC 
CAGCTCCCCG TGGACCCTCA TCGGGACACC ATCCTCAATG CCATCGAGCA GCACCCAGTG 
GTGGTCATTG CTGGGGACAC GGGCTGTGGG AAGACCACGC GCATCCCCCA GCTGCTGCTG 
GAGCGCTACG TGACCGAGGG CCGCGGTGCG CGCTGCAACG TGATCATCAC CCAGCCACGC 
CGCATCTCGG CCGTGTCTGT GGCGCAGCGG GTCAGCCACG AACTGGGCCC TTCCCTGCGC 
CGGAACGTAG GCTTCCAGGT GCGGTTGGAA AGCAAGCCTC CGGCCCGAGG AGGGGCCCTG 
CTGTTCTGCA CGGTGGGCAT CCTGCTGCGG AAGCTGCAGA GCAACCCCAG CCTGGAGGGC 
GTGAGCCACG TGGTCGTGGA CGAGGTGCAC GAGCGAGACG TGAACACGGA CTTCCTGCTG 
ATCCTGCTCA AGGGCCTGCA GCGGCTCAAC CCGGCTCTGC GCTTGGTGCT CATGAGCGCC 
ACTGGCGACA ACGAGCGTTT CTCCCGCTAC TTTGGTGGCT GCCCTGTCAT CAAGGTCCCC 
GGCTTCATGT ACCCTGTCAA GGAGCACTAC CTGGAGGACA TCCTGGCCAA GCTGGGCAAG 
CACCAGTATC CGCACCGGCA CCGGCACCAC GAGTCTGAGG ATGAATGCGC TCTCGATTTG 
GACCTCGTGA CTGATCTGGT TCTGCACATC GATGCCCGCG GGGAACCAGG TGGGATCCTC 
TGCTTCCTGC CGGGCTGGCA GGAGATCAAA GGAGTCCAGC AACGCCTCCA GGAGGCCCTG 
GGCATGCACG AGAGCAAGTA CCTCATCCTG CCAGTGCACT CCAACATCCC GATGATGGAC 
CAGAAGGCCA TATTCCAGCA GCCTCCCATT GGGGTACGCA AGATCGTCTT GGCCACCAAC 
ATTGCCGAGA CGTCCATCAC GATCAATGAC ATCGTGCACG TGGTGGACAG CGGTCTGCAC 
AAGGAGGAGC GCTACGACCT GAAGACCAAG GTTTCCTGCC TGGAGACCGT GTGGGTGTCA 
CGAGCCAATG TGATCCAGCG CCGGGGCCGG GCGGGCCGCT GCCAGTCTGG CTTTGCCTAC 
CACCTCTTCC CACGGAGCCG GCTGGAGAAG ATGGCTCCTT TCCAAGTGCC AGAGATCCTG 
CGCACACCCC TGGAGAACCT GGTGCTGCAG GCCAAGATCC ACATGCCAGA GAAGACGGCA 
GTGGAGTTCC TCTCCAAGGC CGTAGACAGT CCAAACATCA AGGCGGTGGA CGAGGCCGTG 
ATCTTGCTCC AAGAGATTGG GGTTCTGGAC CAGCGGGAGT ACCTGACCAC CCTGGGGCAG 
CGCCTGGCCC ACATCTCCAC TGACCCGCGG CTGGCCAAGG CCATCGTGCT GGCTGCCATC 
TTCCGTTGCC TGCACCCGCT GTTAGTGGTC GTCTCGTGCC TCACCCGGGA CCCCTTCAGC 
AGCAGCCTGC AGAACCGGGC GGAGGTGGAC AAGGTGAAAG CACTGTTGAG CCACGACAGC 
GGCAGTGACC ACCTGGCCTT TGTGCGGGCG GTGTCCGGCT GGGAGGAGGT GCTGCGCTGG 
CAGGATCGCA GCTCCCGTGA GAACTACCTG GAGGAAAACC TGCTCTACGC ACCCAGCCTG 
CGCTTCATCC ACGGACTCAT CAAGCAGTTC TCAGAGAACA TTTATGAGGC TTTCCTGGTT 
GGGAAGCCCT CGGACTGCAC CCTGGCCTCT GCCCAGTGCA ACGAGTACAG TGAGGAGGAG 
GAGCTGGTGA AGGGGGTGCT GATGGCGGGT CTCTACCCCA ACCTCATCCA GGTGAGGCAG 
GGCAAGGTGA CCCGGCAGGG CAAGTTCAAG CCCAACAGTG TCACGTACAG GACCAAATCC 
GGCAACATCT TGCTGCACAA GTCGACCATT AACAGGGAGG CCACCCGGCT GCGGAGCCGA 
TGGCTGACGT ATTTCATGGC TGTCAAGTCC AACGGCAGCG TCTTCGTCCG GGACTCCTCC 
CAGGTGCACC CACTAGCTGT GCTGCTACTG ACCGACGGGG ACGTCCACAT CCGTGATGAC 
GGGCGCCGGG CCACCATTTC CCTGAGCGAC AGTGACCTGC TGCGGCTGGA GGGTGACTCG 
CGCACCGTGC GGCTGCTGCG GGAGCTGCGC CGGGCCCTGG GCCGCATGGT GGAGCGGAGC 
CTGCGCAGCG AGCTGGCGGC ACTGCCGCCC TGTGTGCAGG AGGAGCATGG GCAGCTGCTC 
GCCCTGCTGG CGGAGCTGCT GCGCGGGCCC TGCGGCAGCT TTGATGTGCG CAAGACCGCT 
GACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025128432.1
CDS	165..3653
Translation	MFSLDSFRKASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGSLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHAPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWISSELRLQGEDILPLGKDSGPLSDPITGKPYVPLSEAEELRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVIAGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVVVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPIGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMAPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVSGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLRELRRALGRMVERSLRSELAALPPCVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_025272647.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025272647.1

ORF Insert Sequence:

ATGTTCAGCC TGGACTCATT CAGGAAAGCT TCTAGGGACC TATTAAAAGA GTTTCCACAG 
CCCAAAAATC TTCTCAACAG TGTGATTGGA AGAGCACTTG GCATCTCACA CGCAAAAGAC 
AAATTGGTCT ACGTGCATAC AAATGGACCG AAGAAAAAGA AAGTCACCCT CCACATAAAG 
TGGCCCAAGA GCGTGGAGGT AGAAGGCTAT GGCAGCAAGA AGATCGATGC CGAGAGGCAG 
GCTGCAGCTG CGGCCTGCCA GCTGTTCAAG GGCTGGGGTC TGCTGGGTCC CCGGAATGAG 
CTGTTTGATG CAGCCAAATA CCGCGTGCTA GCCGATCGCT TTGGCTCTCC GGCTGACAGC 
TGGTGGCGCC CAGAACCCAC CATGCCACCC ACTTCCTGGC GGCAGCTGAA TCCTGAGAGC 
ATCCGGCCAG GGGGACCTGG GAGCCTGTCC CGCTCCTTGG GTCGGGAGGA AGAGGAGGAT 
GAGGAGGAAG AACTAGAAGA AGGGACCATC GATGTCACTG AATTTCTGTC TATGACCCAG 
CAGGACTCCC ACGCCCCACT CAGGGATTCC AGGGGGGGTT CCTTTGAAAT GACAGATGAC 
GACAGTGCTA TCAGGGCTCT GACCCAGTTT CCACTTCCCA AGAACCTTCT GGCCAAGGTG 
ATTCAGATAG CAACATCCTC CTCCACAGCC AAGAACCTCA TGCAGTTCCA CACCGTGGGC 
ACCAAGACCA AGCTGTCCAC CCTCACCCTG CTCTGGCCCT GTCCCATGAC CTTCGTGGCC 
AAGGGGCGTC GCAAAGCAGA GGCAGAGAAT AAAGCGGCAG CCTTGGCTTG TAAGAAACTG 
AAGAGCCTGG GACTGGTGGA CCGGAACAAC GAGCCGCTCA CCCACGCCAT GTATAACTTG 
GCCTCCTTGC GAGAGCTGGG TGAGACCCAG CGCCGGCCGT GTACCATCCA GGTGCCTGAG 
CCCATCCTCC GCAAGATCGA GACCTTCCTT AACCATTACC CTGTGGAGAG TTCGTGGATC 
TCCTCAGAGC TCCGGCTGCA GGGTGAGGAC ATCCTGCCCT TGGGCAAGGA CTCGGGGCCC 
CTGAGTGACC CCATCACAGG CAAGCCCTAT GTGCCACTGT CAGAAGCAGA GGAGCTGCGT 
CTGAGCCAGA GTCTGCTGGA GCTGTGGCGG CGGCGAGGGC CAGTCTGGCA GGAGGCACCC 
CAGCTCCCCG TGGACCCTCA TCGGGACACC ATCCTCAATG CCATCGAGCA GCACCCAGTG 
GTGGTCATTG CTGGGGACAC GGGCTGTGGG AAGACCACGC GCATCCCCCA GCTGCTGCTG 
GAGCGCTACG TGACCGAGGG CCGCGGTGCG CGCTGCAACG TGATCATCAC CCAGCCACGC 
CGCATCTCGG CCGTGTCTGT GGCGCAGCGG GTCAGCCACG AACTGGGCCC TTCCCTGCGC 
CGGAACGTAG GCTTCCAGGT GCGGTTGGAA AGCAAGCCTC CGGCCCGAGG AGGGGCCCTG 
CTGTTCTGCA CGGTGGGCAT CCTGCTGCGG AAGCTGCAGA GCAACCCCAG CCTGGAGGGC 
GTGAGCCACG TGGTCGTGGA CGAGGTGCAC GAGCGAGACG TGAACACGGA CTTCCTGCTG 
ATCCTGCTCA AGGGCCTGCA GCGGCTCAAC CCGGCTCTGC GCTTGGTGCT CATGAGCGCC 
ACTGGCGACA ACGAGCGTTT CTCCCGCTAC TTTGGTGGCT GCCCTGTCAT CAAGGTCCCC 
GGCTTCATGT ACCCTGTCAA GGAGCACTAC CTGGAGGACA TCCTGGCCAA GCTGGGCAAG 
CACCAGTATC CGCACCGGCA CCGGCACCAC GAGTCTGAGG ATGAATGCGC TCTCGATTTG 
GACCTCGTGA CTGATCTGGT TCTGCACATC GATGCCCGCG GGGAACCAGG TGGGATCCTC 
TGCTTCCTGC CGGGCTGGCA GGAGATCAAA GGAGTCCAGC AACGCCTCCA GGAGGCCCTG 
GGCATGCACG AGAGCAAGTA CCTCATCCTG CCAGTGCACT CCAACATCCC GATGATGGAC 
CAGAAGGCCA TATTCCAGCA GCCTCCCATT GGGGTACGCA AGATCGTCTT GGCCACCAAC 
ATTGCCGAGA CGTCCATCAC GATCAATGAC ATCGTGCACG TGGTGGACAG CGGTCTGCAC 
AAGGAGGAGC GCTACGACCT GAAGACCAAG GTTTCCTGCC TGGAGACCGT GTGGGTGTCA 
CGAGCCAATG TGATCCAGCG CCGGGGCCGG GCGGGCCGCT GCCAGTCTGG CTTTGCCTAC 
CACCTCTTCC CACGGAGCCG GCTGGAGAAG ATGGCTCCTT TCCAAGTGCC AGAGATCCTG 
CGCACACCCC TGGAGAACCT GGTGCTGCAG GCCAAGATCC ACATGCCAGA GAAGACGGCA 
GTGGAGTTCC TCTCCAAGGC CGTAGACAGT CCAAACATCA AGGCGGTGGA CGAGGCCGTG 
ATCTTGCTCC AAGAGATTGG GGTTCTGGAC CAGCGGGAGT ACCTGACCAC CCTGGGGCAG 
CGCCTGGCCC ACATCTCCAC TGACCCGCGG CTGGCCAAGG CCATCGTGCT GGCTGCCATC 
TTCCGTTGCC TGCACCCGCT GTTAGTGGTC GTCTCGTGCC TCACCCGGGA CCCCTTCAGC 
AGCAGCCTGC AGAACCGGGC GGAGGTGGAC AAGGTGAAAG CACTGTTGAG CCACGACAGC 
GGCAGTGACC ACCTGGCCTT TGTGCGGGCG GTGTCCGGCT GGGAGGAGGT GCTGCGCTGG 
CAGGATCGCA GCTCCCGTGA GAACTACCTG GAGGAAAACC TGCTCTACGC ACCCAGCCTG 
CGCTTCATCC ACGGACTCAT CAAGCAGTTC TCAGAGAACA TTTATGAGGC TTTCCTGGTT 
GGGAAGCCCT CGGACTGCAC CCTGGCCTCT GCCCAGTGCA ACGAGTACAG TGAGGAGGAG 
GAGCTGGTGA AGGGGGTGCT GATGGCGGGT CTCTACCCCA ACCTCATCCA GGTGAGGCAG 
GGCAAGGTGA CCCGGCAGGG CAAGTTCAAG CCCAACAGTG TCACGTACAG GACCAAATCC 
GGCAACATCT TGCTGCACAA GTCGACCATT AACAGGGAGG CCACCCGGCT GCGGAGCCGA 
TGGCTGACGT ATTTCATGGC TGTCAAGTCC AACGGCAGCG TCTTCGTCCG GGACTCCTCC 
CAGGTGCACC CACTAGCTGT GCTGCTACTG ACCGACGGGG ACGTCCACAT CCGTGATGAC 
GGGCGCCGGG CCACCATTTC CCTGAGCGAC AGTGACCTGC TGCGGCTGGA GGGTGACTCG 
CGCACCGTGC GGCTGCTGCG GGAGCTGCGC CGGGCCCTGG GCCGCATGGT GGAGCGGAGC 
CTGCGCAGCG AGCTGGCGGC ACTGCCGCCC TGTGTGCAGG AGGAGCATGG GCAGCTGCTC 
GCCCTGCTGG CGGAGCTGCT GCGCGGGCCC TGCGGCAGCT TTGATGTGCG CAAGACCGCT 
GACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_025272648.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025128433.1 Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X6, mRNA.

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CloneID	OBu57931
Clone ID Related Accession (Same CDS sequence)	XM_025272647.1 , XM_025272648.1
Accession Version	XM_025272648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	putative ATP-dependent RNA helicase DHX30 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCC TGGACTCATT CAGGAAAGCT TCTAGGGACC TATTAAAAGA GTTTCCACAG 
CCCAAAAATC TTCTCAACAG TGTGATTGGA AGAGCACTTG GCATCTCACA CGCAAAAGAC 
AAATTGGTCT ACGTGCATAC AAATGGACCG AAGAAAAAGA AAGTCACCCT CCACATAAAG 
TGGCCCAAGA GCGTGGAGGT AGAAGGCTAT GGCAGCAAGA AGATCGATGC CGAGAGGCAG 
GCTGCAGCTG CGGCCTGCCA GCTGTTCAAG GGCTGGGGTC TGCTGGGTCC CCGGAATGAG 
CTGTTTGATG CAGCCAAATA CCGCGTGCTA GCCGATCGCT TTGGCTCTCC GGCTGACAGC 
TGGTGGCGCC CAGAACCCAC CATGCCACCC ACTTCCTGGC GGCAGCTGAA TCCTGAGAGC 
ATCCGGCCAG GGGGACCTGG GAGCCTGTCC CGCTCCTTGG GTCGGGAGGA AGAGGAGGAT 
GAGGAGGAAG AACTAGAAGA AGGGACCATC GATGTCACTG AATTTCTGTC TATGACCCAG 
CAGGACTCCC ACGCCCCACT CAGGGATTCC AGGGGGGGTT CCTTTGAAAT GACAGATGAC 
GACAGTGCTA TCAGGGCTCT GACCCAGTTT CCACTTCCCA AGAACCTTCT GGCCAAGGTG 
ATTCAGATAG CAACATCCTC CTCCACAGCC AAGAACCTCA TGCAGTTCCA CACCGTGGGC 
ACCAAGACCA AGCTGTCCAC CCTCACCCTG CTCTGGCCCT GTCCCATGAC CTTCGTGGCC 
AAGGGGCGTC GCAAAGCAGA GGCAGAGAAT AAAGCGGCAG CCTTGGCTTG TAAGAAACTG 
AAGAGCCTGG GACTGGTGGA CCGGAACAAC GAGCCGCTCA CCCACGCCAT GTATAACTTG 
GCCTCCTTGC GAGAGCTGGG TGAGACCCAG CGCCGGCCGT GTACCATCCA GGTGCCTGAG 
CCCATCCTCC GCAAGATCGA GACCTTCCTT AACCATTACC CTGTGGAGAG TTCGTGGATC 
TCCTCAGAGC TCCGGCTGCA GGGTGAGGAC ATCCTGCCCT TGGGCAAGGA CTCGGGGCCC 
CTGAGTGACC CCATCACAGG CAAGCCCTAT GTGCCACTGT CAGAAGCAGA GGAGCTGCGT 
CTGAGCCAGA GTCTGCTGGA GCTGTGGCGG CGGCGAGGGC CAGTCTGGCA GGAGGCACCC 
CAGCTCCCCG TGGACCCTCA TCGGGACACC ATCCTCAATG CCATCGAGCA GCACCCAGTG 
GTGGTCATTG CTGGGGACAC GGGCTGTGGG AAGACCACGC GCATCCCCCA GCTGCTGCTG 
GAGCGCTACG TGACCGAGGG CCGCGGTGCG CGCTGCAACG TGATCATCAC CCAGCCACGC 
CGCATCTCGG CCGTGTCTGT GGCGCAGCGG GTCAGCCACG AACTGGGCCC TTCCCTGCGC 
CGGAACGTAG GCTTCCAGGT GCGGTTGGAA AGCAAGCCTC CGGCCCGAGG AGGGGCCCTG 
CTGTTCTGCA CGGTGGGCAT CCTGCTGCGG AAGCTGCAGA GCAACCCCAG CCTGGAGGGC 
GTGAGCCACG TGGTCGTGGA CGAGGTGCAC GAGCGAGACG TGAACACGGA CTTCCTGCTG 
ATCCTGCTCA AGGGCCTGCA GCGGCTCAAC CCGGCTCTGC GCTTGGTGCT CATGAGCGCC 
ACTGGCGACA ACGAGCGTTT CTCCCGCTAC TTTGGTGGCT GCCCTGTCAT CAAGGTCCCC 
GGCTTCATGT ACCCTGTCAA GGAGCACTAC CTGGAGGACA TCCTGGCCAA GCTGGGCAAG 
CACCAGTATC CGCACCGGCA CCGGCACCAC GAGTCTGAGG ATGAATGCGC TCTCGATTTG 
GACCTCGTGA CTGATCTGGT TCTGCACATC GATGCCCGCG GGGAACCAGG TGGGATCCTC 
TGCTTCCTGC CGGGCTGGCA GGAGATCAAA GGAGTCCAGC AACGCCTCCA GGAGGCCCTG 
GGCATGCACG AGAGCAAGTA CCTCATCCTG CCAGTGCACT CCAACATCCC GATGATGGAC 
CAGAAGGCCA TATTCCAGCA GCCTCCCATT GGGGTACGCA AGATCGTCTT GGCCACCAAC 
ATTGCCGAGA CGTCCATCAC GATCAATGAC ATCGTGCACG TGGTGGACAG CGGTCTGCAC 
AAGGAGGAGC GCTACGACCT GAAGACCAAG GTTTCCTGCC TGGAGACCGT GTGGGTGTCA 
CGAGCCAATG TGATCCAGCG CCGGGGCCGG GCGGGCCGCT GCCAGTCTGG CTTTGCCTAC 
CACCTCTTCC CACGGAGCCG GCTGGAGAAG ATGGCTCCTT TCCAAGTGCC AGAGATCCTG 
CGCACACCCC TGGAGAACCT GGTGCTGCAG GCCAAGATCC ACATGCCAGA GAAGACGGCA 
GTGGAGTTCC TCTCCAAGGC CGTAGACAGT CCAAACATCA AGGCGGTGGA CGAGGCCGTG 
ATCTTGCTCC AAGAGATTGG GGTTCTGGAC CAGCGGGAGT ACCTGACCAC CCTGGGGCAG 
CGCCTGGCCC ACATCTCCAC TGACCCGCGG CTGGCCAAGG CCATCGTGCT GGCTGCCATC 
TTCCGTTGCC TGCACCCGCT GTTAGTGGTC GTCTCGTGCC TCACCCGGGA CCCCTTCAGC 
AGCAGCCTGC AGAACCGGGC GGAGGTGGAC AAGGTGAAAG CACTGTTGAG CCACGACAGC 
GGCAGTGACC ACCTGGCCTT TGTGCGGGCG GTGTCCGGCT GGGAGGAGGT GCTGCGCTGG 
CAGGATCGCA GCTCCCGTGA GAACTACCTG GAGGAAAACC TGCTCTACGC ACCCAGCCTG 
CGCTTCATCC ACGGACTCAT CAAGCAGTTC TCAGAGAACA TTTATGAGGC TTTCCTGGTT 
GGGAAGCCCT CGGACTGCAC CCTGGCCTCT GCCCAGTGCA ACGAGTACAG TGAGGAGGAG 
GAGCTGGTGA AGGGGGTGCT GATGGCGGGT CTCTACCCCA ACCTCATCCA GGTGAGGCAG 
GGCAAGGTGA CCCGGCAGGG CAAGTTCAAG CCCAACAGTG TCACGTACAG GACCAAATCC 
GGCAACATCT TGCTGCACAA GTCGACCATT AACAGGGAGG CCACCCGGCT GCGGAGCCGA 
TGGCTGACGT ATTTCATGGC TGTCAAGTCC AACGGCAGCG TCTTCGTCCG GGACTCCTCC 
CAGGTGCACC CACTAGCTGT GCTGCTACTG ACCGACGGGG ACGTCCACAT CCGTGATGAC 
GGGCGCCGGG CCACCATTTC CCTGAGCGAC AGTGACCTGC TGCGGCTGGA GGGTGACTCG 
CGCACCGTGC GGCTGCTGCG GGAGCTGCGC CGGGCCCTGG GCCGCATGGT GGAGCGGAGC 
CTGCGCAGCG AGCTGGCGGC ACTGCCGCCC TGTGTGCAGG AGGAGCATGG GCAGCTGCTC 
GCCCTGCTGG CGGAGCTGCT GCGCGGGCCC TGCGGCAGCT TTGATGTGCG CAAGACCGCT 
GACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025128433.1
CDS	259..3747
Translation	MFSLDSFRKASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGSLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHAPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWISSELRLQGEDILPLGKDSGPLSDPITGKPYVPLSEAEELRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVIAGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVVVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPIGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMAPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVSGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLRELRRALGRMVERSLRSELAALPPCVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_025272648.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025272648.1

ORF Insert Sequence:

ATGTTCAGCC TGGACTCATT CAGGAAAGCT TCTAGGGACC TATTAAAAGA GTTTCCACAG 
CCCAAAAATC TTCTCAACAG TGTGATTGGA AGAGCACTTG GCATCTCACA CGCAAAAGAC 
AAATTGGTCT ACGTGCATAC AAATGGACCG AAGAAAAAGA AAGTCACCCT CCACATAAAG 
TGGCCCAAGA GCGTGGAGGT AGAAGGCTAT GGCAGCAAGA AGATCGATGC CGAGAGGCAG 
GCTGCAGCTG CGGCCTGCCA GCTGTTCAAG GGCTGGGGTC TGCTGGGTCC CCGGAATGAG 
CTGTTTGATG CAGCCAAATA CCGCGTGCTA GCCGATCGCT TTGGCTCTCC GGCTGACAGC 
TGGTGGCGCC CAGAACCCAC CATGCCACCC ACTTCCTGGC GGCAGCTGAA TCCTGAGAGC 
ATCCGGCCAG GGGGACCTGG GAGCCTGTCC CGCTCCTTGG GTCGGGAGGA AGAGGAGGAT 
GAGGAGGAAG AACTAGAAGA AGGGACCATC GATGTCACTG AATTTCTGTC TATGACCCAG 
CAGGACTCCC ACGCCCCACT CAGGGATTCC AGGGGGGGTT CCTTTGAAAT GACAGATGAC 
GACAGTGCTA TCAGGGCTCT GACCCAGTTT CCACTTCCCA AGAACCTTCT GGCCAAGGTG 
ATTCAGATAG CAACATCCTC CTCCACAGCC AAGAACCTCA TGCAGTTCCA CACCGTGGGC 
ACCAAGACCA AGCTGTCCAC CCTCACCCTG CTCTGGCCCT GTCCCATGAC CTTCGTGGCC 
AAGGGGCGTC GCAAAGCAGA GGCAGAGAAT AAAGCGGCAG CCTTGGCTTG TAAGAAACTG 
AAGAGCCTGG GACTGGTGGA CCGGAACAAC GAGCCGCTCA CCCACGCCAT GTATAACTTG 
GCCTCCTTGC GAGAGCTGGG TGAGACCCAG CGCCGGCCGT GTACCATCCA GGTGCCTGAG 
CCCATCCTCC GCAAGATCGA GACCTTCCTT AACCATTACC CTGTGGAGAG TTCGTGGATC 
TCCTCAGAGC TCCGGCTGCA GGGTGAGGAC ATCCTGCCCT TGGGCAAGGA CTCGGGGCCC 
CTGAGTGACC CCATCACAGG CAAGCCCTAT GTGCCACTGT CAGAAGCAGA GGAGCTGCGT 
CTGAGCCAGA GTCTGCTGGA GCTGTGGCGG CGGCGAGGGC CAGTCTGGCA GGAGGCACCC 
CAGCTCCCCG TGGACCCTCA TCGGGACACC ATCCTCAATG CCATCGAGCA GCACCCAGTG 
GTGGTCATTG CTGGGGACAC GGGCTGTGGG AAGACCACGC GCATCCCCCA GCTGCTGCTG 
GAGCGCTACG TGACCGAGGG CCGCGGTGCG CGCTGCAACG TGATCATCAC CCAGCCACGC 
CGCATCTCGG CCGTGTCTGT GGCGCAGCGG GTCAGCCACG AACTGGGCCC TTCCCTGCGC 
CGGAACGTAG GCTTCCAGGT GCGGTTGGAA AGCAAGCCTC CGGCCCGAGG AGGGGCCCTG 
CTGTTCTGCA CGGTGGGCAT CCTGCTGCGG AAGCTGCAGA GCAACCCCAG CCTGGAGGGC 
GTGAGCCACG TGGTCGTGGA CGAGGTGCAC GAGCGAGACG TGAACACGGA CTTCCTGCTG 
ATCCTGCTCA AGGGCCTGCA GCGGCTCAAC CCGGCTCTGC GCTTGGTGCT CATGAGCGCC 
ACTGGCGACA ACGAGCGTTT CTCCCGCTAC TTTGGTGGCT GCCCTGTCAT CAAGGTCCCC 
GGCTTCATGT ACCCTGTCAA GGAGCACTAC CTGGAGGACA TCCTGGCCAA GCTGGGCAAG 
CACCAGTATC CGCACCGGCA CCGGCACCAC GAGTCTGAGG ATGAATGCGC TCTCGATTTG 
GACCTCGTGA CTGATCTGGT TCTGCACATC GATGCCCGCG GGGAACCAGG TGGGATCCTC 
TGCTTCCTGC CGGGCTGGCA GGAGATCAAA GGAGTCCAGC AACGCCTCCA GGAGGCCCTG 
GGCATGCACG AGAGCAAGTA CCTCATCCTG CCAGTGCACT CCAACATCCC GATGATGGAC 
CAGAAGGCCA TATTCCAGCA GCCTCCCATT GGGGTACGCA AGATCGTCTT GGCCACCAAC 
ATTGCCGAGA CGTCCATCAC GATCAATGAC ATCGTGCACG TGGTGGACAG CGGTCTGCAC 
AAGGAGGAGC GCTACGACCT GAAGACCAAG GTTTCCTGCC TGGAGACCGT GTGGGTGTCA 
CGAGCCAATG TGATCCAGCG CCGGGGCCGG GCGGGCCGCT GCCAGTCTGG CTTTGCCTAC 
CACCTCTTCC CACGGAGCCG GCTGGAGAAG ATGGCTCCTT TCCAAGTGCC AGAGATCCTG 
CGCACACCCC TGGAGAACCT GGTGCTGCAG GCCAAGATCC ACATGCCAGA GAAGACGGCA 
GTGGAGTTCC TCTCCAAGGC CGTAGACAGT CCAAACATCA AGGCGGTGGA CGAGGCCGTG 
ATCTTGCTCC AAGAGATTGG GGTTCTGGAC CAGCGGGAGT ACCTGACCAC CCTGGGGCAG 
CGCCTGGCCC ACATCTCCAC TGACCCGCGG CTGGCCAAGG CCATCGTGCT GGCTGCCATC 
TTCCGTTGCC TGCACCCGCT GTTAGTGGTC GTCTCGTGCC TCACCCGGGA CCCCTTCAGC 
AGCAGCCTGC AGAACCGGGC GGAGGTGGAC AAGGTGAAAG CACTGTTGAG CCACGACAGC 
GGCAGTGACC ACCTGGCCTT TGTGCGGGCG GTGTCCGGCT GGGAGGAGGT GCTGCGCTGG 
CAGGATCGCA GCTCCCGTGA GAACTACCTG GAGGAAAACC TGCTCTACGC ACCCAGCCTG 
CGCTTCATCC ACGGACTCAT CAAGCAGTTC TCAGAGAACA TTTATGAGGC TTTCCTGGTT 
GGGAAGCCCT CGGACTGCAC CCTGGCCTCT GCCCAGTGCA ACGAGTACAG TGAGGAGGAG 
GAGCTGGTGA AGGGGGTGCT GATGGCGGGT CTCTACCCCA ACCTCATCCA GGTGAGGCAG 
GGCAAGGTGA CCCGGCAGGG CAAGTTCAAG CCCAACAGTG TCACGTACAG GACCAAATCC 
GGCAACATCT TGCTGCACAA GTCGACCATT AACAGGGAGG CCACCCGGCT GCGGAGCCGA 
TGGCTGACGT ATTTCATGGC TGTCAAGTCC AACGGCAGCG TCTTCGTCCG GGACTCCTCC 
CAGGTGCACC CACTAGCTGT GCTGCTACTG ACCGACGGGG ACGTCCACAT CCGTGATGAC 
GGGCGCCGGG CCACCATTTC CCTGAGCGAC AGTGACCTGC TGCGGCTGGA GGGTGACTCG 
CGCACCGTGC GGCTGCTGCG GGAGCTGCGC CGGGCCCTGG GCCGCATGGT GGAGCGGAGC 
CTGCGCAGCG AGCTGGCGGC ACTGCCGCCC TGTGTGCAGG AGGAGCATGG GCAGCTGCTC 
GCCCTGCTGG CGGAGCTGCT GCGCGGGCCC TGCGGCAGCT TTGATGTGCG CAAGACCGCT 
GACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_025272650.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025128435.1 Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X7, mRNA.

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CloneID	OBu57932
Clone ID Related Accession (Same CDS sequence)	XM_025272650.1
Accession Version	XM_025272650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	putative ATP-dependent RNA helicase DHX30 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCCA CTTCCTGGCG GCAGCTGAAT CCTGAGAGCA TCCGGCCAGG GGGACCTGGG 
AGCCTGTCCC GCTCCTTGGG TCGGGAGGAA GAGGAGGATG AGGAGGAAGA ACTAGAAGAA 
GGGACCATCG ATGTCACTGA ATTTCTGTCT ATGACCCAGC AGGACTCCCA CGCCCCACTC 
AGGGATTCCA GGGGGGGTTC CTTTGAAATG ACAGATGACG ACAGTGCTAT CAGGGCTCTG 
ACCCAGTTTC CACTTCCCAA GAACCTTCTG GCCAAGGTGA TTCAGATAGC AACATCCTCC 
TCCACAGCCA AGAACCTCAT GCAGTTCCAC ACCGTGGGCA CCAAGACCAA GCTGTCCACC 
CTCACCCTGC TCTGGCCCTG TCCCATGACC TTCGTGGCCA AGGGGCGTCG CAAAGCAGAG 
GCAGAGAATA AAGCGGCAGC CTTGGCTTGT AAGAAACTGA AGAGCCTGGG ACTGGTGGAC 
CGGAACAACG AGCCGCTCAC CCACGCCATG TATAACTTGG CCTCCTTGCG AGAGCTGGGT 
GAGACCCAGC GCCGGCCGTG TACCATCCAG GTGCCTGAGC CCATCCTCCG CAAGATCGAG 
ACCTTCCTTA ACCATTACCC TGTGGAGAGT TCGTGGATCT CCTCAGAGCT CCGGCTGCAG 
GGTGAGGACA TCCTGCCCTT GGGCAAGGAC TCGGGGCCCC TGAGTGACCC CATCACAGGC 
AAGCCCTATG TGCCACTGTC AGAAGCAGAG GAGCTGCGTC TGAGCCAGAG TCTGCTGGAG 
CTGTGGCGGC GGCGAGGGCC AGTCTGGCAG GAGGCACCCC AGCTCCCCGT GGACCCTCAT 
CGGGACACCA TCCTCAATGC CATCGAGCAG CACCCAGTGG TGGTCATTGC TGGGGACACG 
GGCTGTGGGA AGACCACGCG CATCCCCCAG CTGCTGCTGG AGCGCTACGT GACCGAGGGC 
CGCGGTGCGC GCTGCAACGT GATCATCACC CAGCCACGCC GCATCTCGGC CGTGTCTGTG 
GCGCAGCGGG TCAGCCACGA ACTGGGCCCT TCCCTGCGCC GGAACGTAGG CTTCCAGGTG 
CGGTTGGAAA GCAAGCCTCC GGCCCGAGGA GGGGCCCTGC TGTTCTGCAC GGTGGGCATC 
CTGCTGCGGA AGCTGCAGAG CAACCCCAGC CTGGAGGGCG TGAGCCACGT GGTCGTGGAC 
GAGGTGCACG AGCGAGACGT GAACACGGAC TTCCTGCTGA TCCTGCTCAA GGGCCTGCAG 
CGGCTCAACC CGGCTCTGCG CTTGGTGCTC ATGAGCGCCA CTGGCGACAA CGAGCGTTTC 
TCCCGCTACT TTGGTGGCTG CCCTGTCATC AAGGTCCCCG GCTTCATGTA CCCTGTCAAG 
GAGCACTACC TGGAGGACAT CCTGGCCAAG CTGGGCAAGC ACCAGTATCC GCACCGGCAC 
CGGCACCACG AGTCTGAGGA TGAATGCGCT CTCGATTTGG ACCTCGTGAC TGATCTGGTT 
CTGCACATCG ATGCCCGCGG GGAACCAGGT GGGATCCTCT GCTTCCTGCC GGGCTGGCAG 
GAGATCAAAG GAGTCCAGCA ACGCCTCCAG GAGGCCCTGG GCATGCACGA GAGCAAGTAC 
CTCATCCTGC CAGTGCACTC CAACATCCCG ATGATGGACC AGAAGGCCAT ATTCCAGCAG 
CCTCCCATTG GGGTACGCAA GATCGTCTTG GCCACCAACA TTGCCGAGAC GTCCATCACG 
ATCAATGACA TCGTGCACGT GGTGGACAGC GGTCTGCACA AGGAGGAGCG CTACGACCTG 
AAGACCAAGG TTTCCTGCCT GGAGACCGTG TGGGTGTCAC GAGCCAATGT GATCCAGCGC 
CGGGGCCGGG CGGGCCGCTG CCAGTCTGGC TTTGCCTACC ACCTCTTCCC ACGGAGCCGG 
CTGGAGAAGA TGGCTCCTTT CCAAGTGCCA GAGATCCTGC GCACACCCCT GGAGAACCTG 
GTGCTGCAGG CCAAGATCCA CATGCCAGAG AAGACGGCAG TGGAGTTCCT CTCCAAGGCC 
GTAGACAGTC CAAACATCAA GGCGGTGGAC GAGGCCGTGA TCTTGCTCCA AGAGATTGGG 
GTTCTGGACC AGCGGGAGTA CCTGACCACC CTGGGGCAGC GCCTGGCCCA CATCTCCACT 
GACCCGCGGC TGGCCAAGGC CATCGTGCTG GCTGCCATCT TCCGTTGCCT GCACCCGCTG 
TTAGTGGTCG TCTCGTGCCT CACCCGGGAC CCCTTCAGCA GCAGCCTGCA GAACCGGGCG 
GAGGTGGACA AGGTGAAAGC ACTGTTGAGC CACGACAGCG GCAGTGACCA CCTGGCCTTT 
GTGCGGGCGG TGTCCGGCTG GGAGGAGGTG CTGCGCTGGC AGGATCGCAG CTCCCGTGAG 
AACTACCTGG AGGAAAACCT GCTCTACGCA CCCAGCCTGC GCTTCATCCA CGGACTCATC 
AAGCAGTTCT CAGAGAACAT TTATGAGGCT TTCCTGGTTG GGAAGCCCTC GGACTGCACC 
CTGGCCTCTG CCCAGTGCAA CGAGTACAGT GAGGAGGAGG AGCTGGTGAA GGGGGTGCTG 
ATGGCGGGTC TCTACCCCAA CCTCATCCAG GTGAGGCAGG GCAAGGTGAC CCGGCAGGGC 
AAGTTCAAGC CCAACAGTGT CACGTACAGG ACCAAATCCG GCAACATCTT GCTGCACAAG 
TCGACCATTA ACAGGGAGGC CACCCGGCTG CGGAGCCGAT GGCTGACGTA TTTCATGGCT 
GTCAAGTCCA ACGGCAGCGT CTTCGTCCGG GACTCCTCCC AGGTGCACCC ACTAGCTGTG 
CTGCTACTGA CCGACGGGGA CGTCCACATC CGTGATGACG GGCGCCGGGC CACCATTTCC 
CTGAGCGACA GTGACCTGCT GCGGCTGGAG GGTGACTCGC GCACCGTGCG GCTGCTGCGG 
GAGCTGCGCC GGGCCCTGGG CCGCATGGTG GAGCGGAGCC TGCGCAGCGA GCTGGCGGCA 
CTGCCGCCCT GTGTGCAGGA GGAGCATGGG CAGCTGCTCG CCCTGCTGGC GGAGCTGCTG 
CGCGGGCCCT GCGGCAGCTT TGATGTGCGC AAGACCGCTG ACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025128435.1
CDS	662..3769
Translation	MPPTSWRQLNPESIRPGGPGSLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHAPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWISSELRLQGEDILPLGKDSGPLSDPITGKPYVPLSEAEELRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVIAGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVVVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPIGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMAPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVSGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLRELRRALGRMVERSLRSELAALPPCVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_025272650.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025272650.1

ORF Insert Sequence:

ATGCCACCCA CTTCCTGGCG GCAGCTGAAT CCTGAGAGCA TCCGGCCAGG GGGACCTGGG 
AGCCTGTCCC GCTCCTTGGG TCGGGAGGAA GAGGAGGATG AGGAGGAAGA ACTAGAAGAA 
GGGACCATCG ATGTCACTGA ATTTCTGTCT ATGACCCAGC AGGACTCCCA CGCCCCACTC 
AGGGATTCCA GGGGGGGTTC CTTTGAAATG ACAGATGACG ACAGTGCTAT CAGGGCTCTG 
ACCCAGTTTC CACTTCCCAA GAACCTTCTG GCCAAGGTGA TTCAGATAGC AACATCCTCC 
TCCACAGCCA AGAACCTCAT GCAGTTCCAC ACCGTGGGCA CCAAGACCAA GCTGTCCACC 
CTCACCCTGC TCTGGCCCTG TCCCATGACC TTCGTGGCCA AGGGGCGTCG CAAAGCAGAG 
GCAGAGAATA AAGCGGCAGC CTTGGCTTGT AAGAAACTGA AGAGCCTGGG ACTGGTGGAC 
CGGAACAACG AGCCGCTCAC CCACGCCATG TATAACTTGG CCTCCTTGCG AGAGCTGGGT 
GAGACCCAGC GCCGGCCGTG TACCATCCAG GTGCCTGAGC CCATCCTCCG CAAGATCGAG 
ACCTTCCTTA ACCATTACCC TGTGGAGAGT TCGTGGATCT CCTCAGAGCT CCGGCTGCAG 
GGTGAGGACA TCCTGCCCTT GGGCAAGGAC TCGGGGCCCC TGAGTGACCC CATCACAGGC 
AAGCCCTATG TGCCACTGTC AGAAGCAGAG GAGCTGCGTC TGAGCCAGAG TCTGCTGGAG 
CTGTGGCGGC GGCGAGGGCC AGTCTGGCAG GAGGCACCCC AGCTCCCCGT GGACCCTCAT 
CGGGACACCA TCCTCAATGC CATCGAGCAG CACCCAGTGG TGGTCATTGC TGGGGACACG 
GGCTGTGGGA AGACCACGCG CATCCCCCAG CTGCTGCTGG AGCGCTACGT GACCGAGGGC 
CGCGGTGCGC GCTGCAACGT GATCATCACC CAGCCACGCC GCATCTCGGC CGTGTCTGTG 
GCGCAGCGGG TCAGCCACGA ACTGGGCCCT TCCCTGCGCC GGAACGTAGG CTTCCAGGTG 
CGGTTGGAAA GCAAGCCTCC GGCCCGAGGA GGGGCCCTGC TGTTCTGCAC GGTGGGCATC 
CTGCTGCGGA AGCTGCAGAG CAACCCCAGC CTGGAGGGCG TGAGCCACGT GGTCGTGGAC 
GAGGTGCACG AGCGAGACGT GAACACGGAC TTCCTGCTGA TCCTGCTCAA GGGCCTGCAG 
CGGCTCAACC CGGCTCTGCG CTTGGTGCTC ATGAGCGCCA CTGGCGACAA CGAGCGTTTC 
TCCCGCTACT TTGGTGGCTG CCCTGTCATC AAGGTCCCCG GCTTCATGTA CCCTGTCAAG 
GAGCACTACC TGGAGGACAT CCTGGCCAAG CTGGGCAAGC ACCAGTATCC GCACCGGCAC 
CGGCACCACG AGTCTGAGGA TGAATGCGCT CTCGATTTGG ACCTCGTGAC TGATCTGGTT 
CTGCACATCG ATGCCCGCGG GGAACCAGGT GGGATCCTCT GCTTCCTGCC GGGCTGGCAG 
GAGATCAAAG GAGTCCAGCA ACGCCTCCAG GAGGCCCTGG GCATGCACGA GAGCAAGTAC 
CTCATCCTGC CAGTGCACTC CAACATCCCG ATGATGGACC AGAAGGCCAT ATTCCAGCAG 
CCTCCCATTG GGGTACGCAA GATCGTCTTG GCCACCAACA TTGCCGAGAC GTCCATCACG 
ATCAATGACA TCGTGCACGT GGTGGACAGC GGTCTGCACA AGGAGGAGCG CTACGACCTG 
AAGACCAAGG TTTCCTGCCT GGAGACCGTG TGGGTGTCAC GAGCCAATGT GATCCAGCGC 
CGGGGCCGGG CGGGCCGCTG CCAGTCTGGC TTTGCCTACC ACCTCTTCCC ACGGAGCCGG 
CTGGAGAAGA TGGCTCCTTT CCAAGTGCCA GAGATCCTGC GCACACCCCT GGAGAACCTG 
GTGCTGCAGG CCAAGATCCA CATGCCAGAG AAGACGGCAG TGGAGTTCCT CTCCAAGGCC 
GTAGACAGTC CAAACATCAA GGCGGTGGAC GAGGCCGTGA TCTTGCTCCA AGAGATTGGG 
GTTCTGGACC AGCGGGAGTA CCTGACCACC CTGGGGCAGC GCCTGGCCCA CATCTCCACT 
GACCCGCGGC TGGCCAAGGC CATCGTGCTG GCTGCCATCT TCCGTTGCCT GCACCCGCTG 
TTAGTGGTCG TCTCGTGCCT CACCCGGGAC CCCTTCAGCA GCAGCCTGCA GAACCGGGCG 
GAGGTGGACA AGGTGAAAGC ACTGTTGAGC CACGACAGCG GCAGTGACCA CCTGGCCTTT 
GTGCGGGCGG TGTCCGGCTG GGAGGAGGTG CTGCGCTGGC AGGATCGCAG CTCCCGTGAG 
AACTACCTGG AGGAAAACCT GCTCTACGCA CCCAGCCTGC GCTTCATCCA CGGACTCATC 
AAGCAGTTCT CAGAGAACAT TTATGAGGCT TTCCTGGTTG GGAAGCCCTC GGACTGCACC 
CTGGCCTCTG CCCAGTGCAA CGAGTACAGT GAGGAGGAGG AGCTGGTGAA GGGGGTGCTG 
ATGGCGGGTC TCTACCCCAA CCTCATCCAG GTGAGGCAGG GCAAGGTGAC CCGGCAGGGC 
AAGTTCAAGC CCAACAGTGT CACGTACAGG ACCAAATCCG GCAACATCTT GCTGCACAAG 
TCGACCATTA ACAGGGAGGC CACCCGGCTG CGGAGCCGAT GGCTGACGTA TTTCATGGCT 
GTCAAGTCCA ACGGCAGCGT CTTCGTCCGG GACTCCTCCC AGGTGCACCC ACTAGCTGTG 
CTGCTACTGA CCGACGGGGA CGTCCACATC CGTGATGACG GGCGCCGGGC CACCATTTCC 
CTGAGCGACA GTGACCTGCT GCGGCTGGAG GGTGACTCGC GCACCGTGCG GCTGCTGCGG 
GAGCTGCGCC GGGCCCTGGG CCGCATGGTG GAGCGGAGCC TGCGCAGCGA GCTGGCGGCA 
CTGCCGCCCT GTGTGCAGGA GGAGCATGGG CAGCTGCTCG CCCTGCTGGC GGAGCTGCTG 
CGCGGGCCCT GCGGCAGCTT TGATGTGCGC AAGACCGCTG ACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_025272651.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025128436.1 Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X8, mRNA.

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CloneID	OBu57933
Clone ID Related Accession (Same CDS sequence)	XM_025272651.1
Accession Version	XM_025272651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	putative ATP-dependent RNA helicase DHX30 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTA GGGACCTATT AAAAGAGTTT CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CACTTGGCAT CTCACACGCA AAAGACAAAT TGGTCTACGT GCATACAAAT 
GGACCGAAGA AAAAGAAAGT CACCCTCCAC ATAAAGTGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCCGAG AGGCAGGCTG CAGCTGCGGC CTGCCAGCTG 
TTCAAGGGCT GGGGTCTGCT GGGTCCCCGG AATGAGCTGT TTGATGCAGC CAAATACCGC 
GTGCTAGCCG ATCGCTTTGG CTCTCCGGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCACCCACTT CCTGGCGGCA GCTGAATCCT GAGAGCATCC GGCCAGGGGG ACCTGGGAGC 
CTGTCCCGCT CCTTGGGTCG GGAGGAAGAG GAGGATGAGG AGGAAGAACT AGAAGAAGGG 
ACCATCGATG TCACTGAATT TCTGTCTATG ACCCAGCAGG ACTCCCACGC CCCACTCAGG 
GATTCCAGGG GGGGTTCCTT TGAAATGACA GATGACGACA GTGCTATCAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATAGCAAC ATCCTCCTCC 
ACAGCCAAGA ACCTCATGCA GTTCCACACC GTGGGCACCA AGACCAAGCT GTCCACCCTC 
ACCCTGCTCT GGCCCTGTCC CATGACCTTC GTGGCCAAGG GGCGTCGCAA AGCAGAGGCA 
GAGAATAAAG CGGCAGCCTT GGCTTGTAAG AAACTGAAGA GCCTGGGACT GGTGGACCGG 
AACAACGAGC CGCTCACCCA CGCCATGTAT AACTTGGCCT CCTTGCGAGA GCTGGGTGAG 
ACCCAGCGCC GGCCGTGTAC CATCCAGGTG CCTGAGCCCA TCCTCCGCAA GATCGAGACC 
TTCCTTAACC ATTACCCTGT GGAGAGTTCG TGGATCTCCT CAGAGCTCCG GCTGCAGGGT 
GAGGACATCC TGCCCTTGGG CAAGGACTCG GGGCCCCTGA GTGACCCCAT CACAGGCAAG 
CCCTATGTGC CACTGTCAGA AGCAGAGGAG CTGCGTCTGA GCCAGAGTCT GCTGGAGCTG 
TGGCGGCGGC GAGGGCCAGT CTGGCAGGAG GCACCCCAGC TCCCCGTGGA CCCTCATCGG 
GACACCATCC TCAATGCCAT CGAGCAGCAC CCAGTGGTGG TCATTGCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG CTGCTGGAGC GCTACGTGAC CGAGGGCCGC 
GGTGCGCGCT GCAACGTGAT CATCACCCAG CCACGCCGCA TCTCGGCCGT GTCTGTGGCG 
CAGCGGGTCA GCCACGAACT GGGCCCTTCC CTGCGCCGGA ACGTAGGCTT CCAGGTGCGG 
TTGGAAAGCA AGCCTCCGGC CCGAGGAGGG GCCCTGCTGT TCTGCACGGT GGGCATCCTG 
CTGCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTGGT CGTGGACGAG 
GTGCACGAGC GAGACGTGAA CACGGACTTC CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CTCTGCGCTT GGTGCTCATG AGCGCCACTG GCGACAACGA GCGTTTCTCC 
CGCTACTTTG GTGGCTGCCC TGTCATCAAG GTCCCCGGCT TCATGTACCC TGTCAAGGAG 
CACTACCTGG AGGACATCCT GGCCAAGCTG GGCAAGCACC AGTATCCGCA CCGGCACCGG 
CACCACGAGT CTGAGGATGA ATGCGCTCTC GATTTGGACC TCGTGACTGA TCTGGTTCTG 
CACATCGATG CCCGCGGGGA ACCAGGTGGG ATCCTCTGCT TCCTGCCGGG CTGGCAGGAG 
ATCAAAGGAG TCCAGCAACG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCGATG ATGGACCAGA AGGCCATATT CCAGCAGCCT 
CCCATTGGGG TACGCAAGAT CGTCTTGGCC ACCAACATTG CCGAGACGTC CATCACGATC 
AATGACATCG TGCACGTGGT GGACAGCGGT CTGCACAAGG AGGAGCGCTA CGACCTGAAG 
ACCAAGGTTT CCTGCCTGGA GACCGTGTGG GTGTCACGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCTGGCTTT GCCTACCACC TCTTCCCACG GAGCCGGCTG 
GAGAAGATGG CTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCCCTGGA GAACCTGGTG 
CTGCAGGCCA AGATCCACAT GCCAGAGAAG ACGGCAGTGG AGTTCCTCTC CAAGGCCGTA 
GACAGTCCAA ACATCAAGGC GGTGGACGAG GCCGTGATCT TGCTCCAAGA GATTGGGGTT 
CTGGACCAGC GGGAGTACCT GACCACCCTG GGGCAGCGCC TGGCCCACAT CTCCACTGAC 
CCGCGGCTGG CCAAGGCCAT CGTGCTGGCT GCCATCTTCC GTTGCCTGCA CCCGCTGTTA 
GTGGTCGTCT CGTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTGCAGAA CCGGGCGGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAC GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCGGTGT CCGGCTGGGA GGAGGTGCTG CGCTGGCAGG ATCGCAGCTC CCGTGAGAAC 
TACCTGGAGG AAAACCTGCT CTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTGGTTGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCTGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG GGTGCTGATG 
GCGGGTCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTGACCCG GCAGGGCAAG 
TTCAAGCCCA ACAGTGTCAC GTACAGGACC AAATCCGGCA ACATCTTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC CCGGCTGCGG AGCCGATGGC TGACGTATTT CATGGCTGTC 
AAGTCCAACG GCAGCGTCTT CGTCCGGGAC TCCTCCCAGG TGCACCCACT AGCTGTGCTG 
CTACTGACCG ACGGGGACGT CCACATCCGT GATGACGGGC GCCGGGCCAC CATTTCCCTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGCA CCGTGCGGCT GCTGCGGGAG 
CTGCGCCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCGGCACTG 
CCGCCCTGTG TGCAGGAGGA GCATGGGCAG CTGCTCGCCC TGCTGGCGGA GCTGCTGCGC 
GGGCCCTGCG GCAGCTTTGA TGTGCGCAAG ACCGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025128436.1
CDS	104..3568
Translation	MASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGSLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHAPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQVPEPILRKIETFLNHYPVESSWISSELRLQGEDILPLGKDSGPLSDPITGKPYVPLSEAEELRLSQSLLELWRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVIAGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQPRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVVVDEVHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYLILPVHSNIPMMDQKAIFQQPPIGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMAPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVSGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLRELRRALGRMVERSLRSELAALPPCVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_025272651.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis DExH-box helicase 30 (DHX30), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025272651.1

ORF Insert Sequence:

ATGGCTTCTA GGGACCTATT AAAAGAGTTT CCACAGCCCA AAAATCTTCT CAACAGTGTG 
ATTGGAAGAG CACTTGGCAT CTCACACGCA AAAGACAAAT TGGTCTACGT GCATACAAAT 
GGACCGAAGA AAAAGAAAGT CACCCTCCAC ATAAAGTGGC CCAAGAGCGT GGAGGTAGAA 
GGCTATGGCA GCAAGAAGAT CGATGCCGAG AGGCAGGCTG CAGCTGCGGC CTGCCAGCTG 
TTCAAGGGCT GGGGTCTGCT GGGTCCCCGG AATGAGCTGT TTGATGCAGC CAAATACCGC 
GTGCTAGCCG ATCGCTTTGG CTCTCCGGCT GACAGCTGGT GGCGCCCAGA ACCCACCATG 
CCACCCACTT CCTGGCGGCA GCTGAATCCT GAGAGCATCC GGCCAGGGGG ACCTGGGAGC 
CTGTCCCGCT CCTTGGGTCG GGAGGAAGAG GAGGATGAGG AGGAAGAACT AGAAGAAGGG 
ACCATCGATG TCACTGAATT TCTGTCTATG ACCCAGCAGG ACTCCCACGC CCCACTCAGG 
GATTCCAGGG GGGGTTCCTT TGAAATGACA GATGACGACA GTGCTATCAG GGCTCTGACC 
CAGTTTCCAC TTCCCAAGAA CCTTCTGGCC AAGGTGATTC AGATAGCAAC ATCCTCCTCC 
ACAGCCAAGA ACCTCATGCA GTTCCACACC GTGGGCACCA AGACCAAGCT GTCCACCCTC 
ACCCTGCTCT GGCCCTGTCC CATGACCTTC GTGGCCAAGG GGCGTCGCAA AGCAGAGGCA 
GAGAATAAAG CGGCAGCCTT GGCTTGTAAG AAACTGAAGA GCCTGGGACT GGTGGACCGG 
AACAACGAGC CGCTCACCCA CGCCATGTAT AACTTGGCCT CCTTGCGAGA GCTGGGTGAG 
ACCCAGCGCC GGCCGTGTAC CATCCAGGTG CCTGAGCCCA TCCTCCGCAA GATCGAGACC 
TTCCTTAACC ATTACCCTGT GGAGAGTTCG TGGATCTCCT CAGAGCTCCG GCTGCAGGGT 
GAGGACATCC TGCCCTTGGG CAAGGACTCG GGGCCCCTGA GTGACCCCAT CACAGGCAAG 
CCCTATGTGC CACTGTCAGA AGCAGAGGAG CTGCGTCTGA GCCAGAGTCT GCTGGAGCTG 
TGGCGGCGGC GAGGGCCAGT CTGGCAGGAG GCACCCCAGC TCCCCGTGGA CCCTCATCGG 
GACACCATCC TCAATGCCAT CGAGCAGCAC CCAGTGGTGG TCATTGCTGG GGACACGGGC 
TGTGGGAAGA CCACGCGCAT CCCCCAGCTG CTGCTGGAGC GCTACGTGAC CGAGGGCCGC 
GGTGCGCGCT GCAACGTGAT CATCACCCAG CCACGCCGCA TCTCGGCCGT GTCTGTGGCG 
CAGCGGGTCA GCCACGAACT GGGCCCTTCC CTGCGCCGGA ACGTAGGCTT CCAGGTGCGG 
TTGGAAAGCA AGCCTCCGGC CCGAGGAGGG GCCCTGCTGT TCTGCACGGT GGGCATCCTG 
CTGCGGAAGC TGCAGAGCAA CCCCAGCCTG GAGGGCGTGA GCCACGTGGT CGTGGACGAG 
GTGCACGAGC GAGACGTGAA CACGGACTTC CTGCTGATCC TGCTCAAGGG CCTGCAGCGG 
CTCAACCCGG CTCTGCGCTT GGTGCTCATG AGCGCCACTG GCGACAACGA GCGTTTCTCC 
CGCTACTTTG GTGGCTGCCC TGTCATCAAG GTCCCCGGCT TCATGTACCC TGTCAAGGAG 
CACTACCTGG AGGACATCCT GGCCAAGCTG GGCAAGCACC AGTATCCGCA CCGGCACCGG 
CACCACGAGT CTGAGGATGA ATGCGCTCTC GATTTGGACC TCGTGACTGA TCTGGTTCTG 
CACATCGATG CCCGCGGGGA ACCAGGTGGG ATCCTCTGCT TCCTGCCGGG CTGGCAGGAG 
ATCAAAGGAG TCCAGCAACG CCTCCAGGAG GCCCTGGGCA TGCACGAGAG CAAGTACCTC 
ATCCTGCCAG TGCACTCCAA CATCCCGATG ATGGACCAGA AGGCCATATT CCAGCAGCCT 
CCCATTGGGG TACGCAAGAT CGTCTTGGCC ACCAACATTG CCGAGACGTC CATCACGATC 
AATGACATCG TGCACGTGGT GGACAGCGGT CTGCACAAGG AGGAGCGCTA CGACCTGAAG 
ACCAAGGTTT CCTGCCTGGA GACCGTGTGG GTGTCACGAG CCAATGTGAT CCAGCGCCGG 
GGCCGGGCGG GCCGCTGCCA GTCTGGCTTT GCCTACCACC TCTTCCCACG GAGCCGGCTG 
GAGAAGATGG CTCCTTTCCA AGTGCCAGAG ATCCTGCGCA CACCCCTGGA GAACCTGGTG 
CTGCAGGCCA AGATCCACAT GCCAGAGAAG ACGGCAGTGG AGTTCCTCTC CAAGGCCGTA 
GACAGTCCAA ACATCAAGGC GGTGGACGAG GCCGTGATCT TGCTCCAAGA GATTGGGGTT 
CTGGACCAGC GGGAGTACCT GACCACCCTG GGGCAGCGCC TGGCCCACAT CTCCACTGAC 
CCGCGGCTGG CCAAGGCCAT CGTGCTGGCT GCCATCTTCC GTTGCCTGCA CCCGCTGTTA 
GTGGTCGTCT CGTGCCTCAC CCGGGACCCC TTCAGCAGCA GCCTGCAGAA CCGGGCGGAG 
GTGGACAAGG TGAAAGCACT GTTGAGCCAC GACAGCGGCA GTGACCACCT GGCCTTTGTG 
CGGGCGGTGT CCGGCTGGGA GGAGGTGCTG CGCTGGCAGG ATCGCAGCTC CCGTGAGAAC 
TACCTGGAGG AAAACCTGCT CTACGCACCC AGCCTGCGCT TCATCCACGG ACTCATCAAG 
CAGTTCTCAG AGAACATTTA TGAGGCTTTC CTGGTTGGGA AGCCCTCGGA CTGCACCCTG 
GCCTCTGCCC AGTGCAACGA GTACAGTGAG GAGGAGGAGC TGGTGAAGGG GGTGCTGATG 
GCGGGTCTCT ACCCCAACCT CATCCAGGTG AGGCAGGGCA AGGTGACCCG GCAGGGCAAG 
TTCAAGCCCA ACAGTGTCAC GTACAGGACC AAATCCGGCA ACATCTTGCT GCACAAGTCG 
ACCATTAACA GGGAGGCCAC CCGGCTGCGG AGCCGATGGC TGACGTATTT CATGGCTGTC 
AAGTCCAACG GCAGCGTCTT CGTCCGGGAC TCCTCCCAGG TGCACCCACT AGCTGTGCTG 
CTACTGACCG ACGGGGACGT CCACATCCGT GATGACGGGC GCCGGGCCAC CATTTCCCTG 
AGCGACAGTG ACCTGCTGCG GCTGGAGGGT GACTCGCGCA CCGTGCGGCT GCTGCGGGAG 
CTGCGCCGGG CCCTGGGCCG CATGGTGGAG CGGAGCCTGC GCAGCGAGCT GGCGGCACTG 
CCGCCCTGTG TGCAGGAGGA GCATGGGCAG CTGCTCGCCC TGCTGGCGGA GCTGCTGCGC 
GGGCCCTGCG GCAGCTTTGA TGTGCGCAAG ACCGCTGACG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_006065227.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065289.1 Bubalus bubalis DEAH (Asp-Glu-Ala-His) box helicase 30 (DHX30), mRNA.

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CloneID	OBu20799
Clone ID Related Accession (Same CDS sequence)	XM_006065227.1
Accession Version	XM_006065227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	putative ATP-dependent RNA helicase DHX30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785163.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCG CCAGGAGACT CATGGCGCTG GCCGCCGGCG TCTCTCCGCG CCTGCGGCCA 
TCGGGTCCCC GCGCCATCGG GCGACAGGGA GGCCCGCGCG GCCTCTCCAC AGGCTGCTCC 
CGCCCCGACC GCACCCAGGA GGCCGCCGAG GCCGAGGCGG CCTCCGGCGA GCCTGGGGAA 
GGCGATGGAA GCGTGGTGAA CGCTTCTAGG GACCTATTAA AAGAGTTTCC ACAGCCCAAA 
AATCTTCTCA ACAGTGTGAT TGGAAGAGCA CTTGGCATCT CACACGCAAA AGACAAATTG 
GTCTACGTGC ATACAAATGG ACCGAAGAAA AAGAAAGTCA CCCTCCACAT AAAGTGGCCC 
AAGAGCGTGG AGGTAGAAGG CTATGGCAGC AAGAAGATCG ATGCCGAGAG GCAGGCTGCA 
GCTGCGGCCT GCCAGCTGTT CAAGGGCTGG GGTCTGCTGG GTCCCCGGAA TGAGCTGTTT 
GATGCAGCCA AATACCGCGT GCTAGCCGAT CGCTTTGGCT CTCCGGCTGA CAGCTGGTGG 
CGCCCAGAAC CCACCATGCC ACCCACTTCC TGGCGGCAGC TGAATCCTGA GAGCATCCGG 
CCAGGGGGAC CTGGGAGCCT GTCCCGCTCC TTGGGTCGGG AGGAAGAGGA GGATGAGGAG 
GAAGAACTAG AAGAAGGGAC CATCGATGTC ACTGAATTTC TGTCTATGAC CCAGCAGGAC 
TCCCACGCCC CACTCAGGGA TTCCAGGGGG GGTTCCTTTG AAATGACAGA TGACGACAGT 
GCTATCAGGG CTCTGACCCA GTTTCCACTT CCCAAGAACC TTCTGGCCAA GGTGATTCAG 
ATAGCAACAT CCTCCTCCAC AGCCAAGAAC CTCATGCAGT TCCACACCGT GGGCACCAAG 
ACCAAGCTGT CCACCCTCAC CCTGCTCTGG CCCTGTCCCA TGACCTTCGT GGCCAAGGGG 
CGTCGCAAAG CAGAGGCAGA GAATAAAGCG GCAGCCTTGG CTTGTAAGAA ACTGAAGGTG 
AGTGCGGAAG GGTCCCTGGT GACGGTGGGC ATCCTGCTGC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GCGTGAGCCA CGTGGTCGTG GACGAGGTGC ACGAGCGAGA CGTGAACACG 
GACTTCCTGC TGATCCTGCT CAAGGGCCTG CAGCGGCTCA ACCCGGCTCT GCGCTTGGTG 
GACAGCGGTC TGCACAAGGA GGAGCGCTAC GACCTGAAGA CCAAGGTTTC CTGCCTGGAG 
ACCGTGTGGG TGTCACGAGC CAATGTGATC CAGCGCCGGG GCCGGGCGGG CCGCTGCCAG 
TCTGGCTTTG CCTACCACCT CTTCCCACGG AGCCGGCTGG AGAAGATGGC TCCTTTCCAA 
GTGCCAGAGA TCCTGCGCAC ACCCCTGGAG AACCTGGTGC TGCAGGCCAA GATCCACATG 
CCAGAGAAGA CGGCAGTGGA GTTCCTCTCC AAGGCCGTAG ACAGTCCAAA CATCAAGGCG 
GTGGACGAGG CCGTGATCTT GCTCCAAGAG ATTGGGGTTC TGGACCAGCG GGAGTACCTG 
ACCACCCTGG GGCAGCGCCT GGCCCACATC TCCACTGACC CGCGGCTGGC CAAGGCCATC 
GTGCTGGCTG CCATCTTCCG TTGCCTGCAC CCGCTGTTAG TGGTCGTCTC GTGCCTCACC 
CGGGACCCCT TCAGCAGCAG CCTGCAGAAC CGGGCGGAGG TGGACAAGGT GAAAGCACTG 
TTGAGCCACG ACAGCGGCAG TGACCACCTG GCCTTTGTGC GGGCGGTGTC CGGCTGGGAG 
GAGGTGCTGC GCTGGCAGGA TCGCAGCTCC CGTGAGAACT ACCTGGAGGA AAACCTGCTC 
TACGCACCCA GCCTGCGCTT CATCCACGGA CTCATCAAGC AGTTCTCAGA GAACATTTAT 
GAGGCTTTCC TGGTTGGGAA GCCCTCGGAC TGCACCCTGG CCTCTGCCCA GTGCAACGAG 
TACAGTGAGG AGGAGGAGCT GGTGAAGGGG GTGCTGATGG CGGGTCTCTA CCCCAACCTC 
ATCCAGGTGA GGCAGGGCAA GGTGACCCGG CAGGGCAAGT TCAAGCCCAA CAGTGTCACG 
TACAGGACCA AATCCGGCAA CATCTTGCTG CACAAGTCGA CCATTAACAG GGAGGCCACC 
CGGCTGCGGA GCCGATGGCT GACGTATTTC ATGGCTGTCA AGTCCAACGG CAGCGTCTTC 
GTCCGGGACT CCTCCCAGGT GCACCCACTA GCTGTGCTGC TACTGACCGA CGGGGACGTC 
CACATCCGTG ATGACGGGCG CCGGGCCACC ATTTCCCTGA GCGACAGTGA CCTGCTGCGG 
CTGGAGGGTG ACTCGCGCAC CGTGCGGCTG CTGCGGGAGC TGCGCCGGGC CCTGGGCCGC 
ATGGTGGAGC GGAGCCTGCG CAGCGAGCTG GCGGCACTGC CGCCCTGTGT GCAGGAGGAG 
CATGGGCAGC TGCTCGCCCT GCTGGCGGAG CTGCTGCGCG GGCCCTGCGG CAGCTTTGAT 
GTGCGCAAGA CCGCTGACGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065289.1
CDS	19..2562
Translation	MATARRLMALAAGVSPRLRPSGPRAIGRQGGPRGLSTGCSRPDRTQEAAEAEAASGEPGEGDGSVVNASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGSLSRSLGREEEEDEEEELEEGTIDVTEFLSMTQQDSHAPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAEAENKAAALACKKLKVSAEGSLVTVGILLRKLQSNPSLEGVSHVVVDEVHERDVNTDFLLILLKGLQRLNPALRLVDSGLHKEERYDLKTKVSCLETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMAPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVSGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVRLLRELRRALGRMVERSLRSELAALPPCVQEEHGQLLALLAELLRGPCGSFDVRKTADD

Target ORF information:

RefSeq Version	XM_006065227.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis DEAH (Asp-Glu-Ala-His) box helicase 30 (DHX30), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006065227.1

ORF Insert Sequence:

ATGGCGACCG CCAGGAGACT CATGGCGCTG GCCGCCGGCG TCTCTCCGCG CCTGCGGCCA 
TCGGGTCCCC GCGCCATCGG GCGACAGGGA GGCCCGCGCG GCCTCTCCAC AGGCTGCTCC 
CGCCCCGACC GCACCCAGGA GGCCGCCGAG GCCGAGGCGG CCTCCGGCGA GCCTGGGGAA 
GGCGATGGAA GCGTGGTGAA CGCTTCTAGG GACCTATTAA AAGAGTTTCC ACAGCCCAAA 
AATCTTCTCA ACAGTGTGAT TGGAAGAGCA CTTGGCATCT CACACGCAAA AGACAAATTG 
GTCTACGTGC ATACAAATGG ACCGAAGAAA AAGAAAGTCA CCCTCCACAT AAAGTGGCCC 
AAGAGCGTGG AGGTAGAAGG CTATGGCAGC AAGAAGATCG ATGCCGAGAG GCAGGCTGCA 
GCTGCGGCCT GCCAGCTGTT CAAGGGCTGG GGTCTGCTGG GTCCCCGGAA TGAGCTGTTT 
GATGCAGCCA AATACCGCGT GCTAGCCGAT CGCTTTGGCT CTCCGGCTGA CAGCTGGTGG 
CGCCCAGAAC CCACCATGCC ACCCACTTCC TGGCGGCAGC TGAATCCTGA GAGCATCCGG 
CCAGGGGGAC CTGGGAGCCT GTCCCGCTCC TTGGGTCGGG AGGAAGAGGA GGATGAGGAG 
GAAGAACTAG AAGAAGGGAC CATCGATGTC ACTGAATTTC TGTCTATGAC CCAGCAGGAC 
TCCCACGCCC CACTCAGGGA TTCCAGGGGG GGTTCCTTTG AAATGACAGA TGACGACAGT 
GCTATCAGGG CTCTGACCCA GTTTCCACTT CCCAAGAACC TTCTGGCCAA GGTGATTCAG 
ATAGCAACAT CCTCCTCCAC AGCCAAGAAC CTCATGCAGT TCCACACCGT GGGCACCAAG 
ACCAAGCTGT CCACCCTCAC CCTGCTCTGG CCCTGTCCCA TGACCTTCGT GGCCAAGGGG 
CGTCGCAAAG CAGAGGCAGA GAATAAAGCG GCAGCCTTGG CTTGTAAGAA ACTGAAGGTG 
AGTGCGGAAG GGTCCCTGGT GACGGTGGGC ATCCTGCTGC GGAAGCTGCA GAGCAACCCC 
AGCCTGGAGG GCGTGAGCCA CGTGGTCGTG GACGAGGTGC ACGAGCGAGA CGTGAACACG 
GACTTCCTGC TGATCCTGCT CAAGGGCCTG CAGCGGCTCA ACCCGGCTCT GCGCTTGGTG 
GACAGCGGTC TGCACAAGGA GGAGCGCTAC GACCTGAAGA CCAAGGTTTC CTGCCTGGAG 
ACCGTGTGGG TGTCACGAGC CAATGTGATC CAGCGCCGGG GCCGGGCGGG CCGCTGCCAG 
TCTGGCTTTG CCTACCACCT CTTCCCACGG AGCCGGCTGG AGAAGATGGC TCCTTTCCAA 
GTGCCAGAGA TCCTGCGCAC ACCCCTGGAG AACCTGGTGC TGCAGGCCAA GATCCACATG 
CCAGAGAAGA CGGCAGTGGA GTTCCTCTCC AAGGCCGTAG ACAGTCCAAA CATCAAGGCG 
GTGGACGAGG CCGTGATCTT GCTCCAAGAG ATTGGGGTTC TGGACCAGCG GGAGTACCTG 
ACCACCCTGG GGCAGCGCCT GGCCCACATC TCCACTGACC CGCGGCTGGC CAAGGCCATC 
GTGCTGGCTG CCATCTTCCG TTGCCTGCAC CCGCTGTTAG TGGTCGTCTC GTGCCTCACC 
CGGGACCCCT TCAGCAGCAG CCTGCAGAAC CGGGCGGAGG TGGACAAGGT GAAAGCACTG 
TTGAGCCACG ACAGCGGCAG TGACCACCTG GCCTTTGTGC GGGCGGTGTC CGGCTGGGAG 
GAGGTGCTGC GCTGGCAGGA TCGCAGCTCC CGTGAGAACT ACCTGGAGGA AAACCTGCTC 
TACGCACCCA GCCTGCGCTT CATCCACGGA CTCATCAAGC AGTTCTCAGA GAACATTTAT 
GAGGCTTTCC TGGTTGGGAA GCCCTCGGAC TGCACCCTGG CCTCTGCCCA GTGCAACGAG 
TACAGTGAGG AGGAGGAGCT GGTGAAGGGG GTGCTGATGG CGGGTCTCTA CCCCAACCTC 
ATCCAGGTGA GGCAGGGCAA GGTGACCCGG CAGGGCAAGT TCAAGCCCAA CAGTGTCACG 
TACAGGACCA AATCCGGCAA CATCTTGCTG CACAAGTCGA CCATTAACAG GGAGGCCACC 
CGGCTGCGGA GCCGATGGCT GACGTATTTC ATGGCTGTCA AGTCCAACGG CAGCGTCTTC 
GTCCGGGACT CCTCCCAGGT GCACCCACTA GCTGTGCTGC TACTGACCGA CGGGGACGTC 
CACATCCGTG ATGACGGGCG CCGGGCCACC ATTTCCCTGA GCGACAGTGA CCTGCTGCGG 
CTGGAGGGTG ACTCGCGCAC CGTGCGGCTG CTGCGGGAGC TGCGCCGGGC CCTGGGCCGC 
ATGGTGGAGC GGAGCCTGCG CAGCGAGCTG GCGGCACTGC CGCCCTGTGT GCAGGAGGAG 
CATGGGCAGC TGCTCGCCCT GCTGGCGGAG CTGCTGCGCG GGCCCTGCGG CAGCTTTGAT 
GTGCGCAAGA CCGCTGACGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DHX30
Entrez Gene ID	102397980
Full Name	DExH-box helicase 30
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DHX30 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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