ARHGEF12 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARHGEF12
Entrez Gene ID	102397446
Full Name	Rho guanine nucleotide exchange factor 12
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006044891.1	XP_006044953.1	LOW QUALITY PROTEIN: rho guanine nucleotide exchange factor 12
XM_025266751.1	XP_025122536.1	rho guanine nucleotide exchange factor 12 isoform X3
XM_025266750.1	XP_025122535.1	rho guanine nucleotide exchange factor 12 isoform X2
XM_006044891.2	XP_006044953.2	rho guanine nucleotide exchange factor 12 isoform X1
XM_025266754.1	XP_025122539.1	rho guanine nucleotide exchange factor 12 isoform X4
XM_025266753.1	XP_025122538.1	rho guanine nucleotide exchange factor 12 isoform X4

Pathways from BioSystems
Gene Ontology

The following ARHGEF12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu50705	XM_025266753.1 Latest version!	Bubalus bubalis Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu50705	XM_025266754.1 Latest version!	Bubalus bubalis Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu03996	XM_006044891.1 Latest version!	Bubalus bubalis Rho guanine nucleotide exchange factor (GEF) 12 (ARHGEF12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu50702	XM_006044891.2 Latest version!	Bubalus bubalis Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu50703	XM_025266750.1 Latest version!	Bubalus bubalis Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu50704	XM_025266751.1 Latest version!	Bubalus bubalis Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARHGEF12 ( XM_025266754.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025122539.1 Bubalus bubalis Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu50705
Clone ID Related Accession (Same CDS sequence)	XM_025266754.1 , XM_025266753.1
Accession Version	XM_025266754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	rho guanine nucleotide exchange factor 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CATTCAAACC ATTTGGAAGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCCCCTGG GTCGCCCCAG ATTCCACTTG CTGATTCTGA AGTAGAGCCA 
TCGGTCACTG GACATATGTC TCCCATCATG ACATCCCCTC ATTCACCTGG TGCACCTGGG 
AATATGGAGA GAATTACTAG TCCTGTGCTC ATGGGGGAAG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT TCAGCTATTG 
CAGGAAGACT ACAACCGAAC ACCTGCTCAG AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CATCCTCCAA GCCGTTAGCT GACGGGCTAG CAGTGGGTGA AGGAGAAGCA 
GATCCTGGTG ATGGGCTAGG CAGGCTTGAC TATGGCAGCG GAGACGCTTC TCGGCCCGGT 
AGTGACAGTG CCGATAGTCC CAAGAGTGGC TTGAAAGAGA GGATTTATCT AGAGGAAAAC 
CCAGAAAAAA GTGAAGCAAT TCAGGACACT GACACTCAGT CGCTTGTTGG GAGTCCCTCT 
ACCCGTGTGG CACCTCATAT TATTGGAGCA GAGGACGATG ACTTTGGTAG TGAACATGAA 
CAGATCAATG GGCAGTGCAG CTGTTTTCAG AGCATTGAGC TGCTAAAATC TCGCCCTGCT 
CACTTGGCTG TCTTCTTACA CCATGTAGTT TCCCAGTTTG ACCCTGCAAC ATTGCTCTGT 
TACCTCTATT CAGACCTATA TAAACAGACC AATTCCAAGG AGACCCGTCG TGTCTTCCTT 
GAGTTCCATC AGTTCTTCCT GGATCGATCT GCTCACCTGA AGGTTTCTGT TCCTGATGAA 
ATATCAGTAG ATCTAGAAAA AAGACGACCA GAGCTTATTC CTGAGGATCT ACATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAC CCAGAAGTTC AGAAGCACTT GGAAGATTTT 
CGGCAGAAGC GCAGCATGGG GCTGACCCTG GCTGAAAGTG AACTGACTAA ACTTGATGTA 
GAGCGAGACA AGGACCGGTT AACTTTGGAG AAGGAACGGG CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTGTT GATGACTGCT CAGGCTGTAG AAGAAGAAAA GAGTTCAACA 
ATGCAATATG TTATTCTCAT GTACATGAAA CATTTGGGAG TAAAAGTGAA AGAACCTCGA 
AATTTGGAGC ACAAGCGAGG TCGGATTGGA TTCCTTCCAA AAATCAAGCA AAGTATGAAG 
AAAGATAGAG AAGGCGAAGA AAAGGGGAAG CGAAGAGGGT TCACCAGCAT CTTGGGACCC 
CCTAGGAGAC CAAGCCGCCA CGACAATAGC GCAATTGGCA GAGCCATGGA ACTGCAGAAG 
CAGCGCCACC CTAAGCACTT GTCCACACCC TCGTCTGTGA GTCCTGAACC TCAGGATTCT 
GCGAAGCTGC GCCAGAGTGG ATCAGCAAGT GAAGGAGCAG ACAGTGGATA CCTGCCAGCC 
AATTCCACGT CCTCCATGGT TTCAGGGGCC ACTCTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATATGGGAT CAAAACAAGC TGGAGAAACG CCAGCATCTG GAGACTCCTT GGATGGCACT 
CCTCGTACTC CCAACACCAT CTTTGATTTC CCACCTCCTC CATTAGACCA GGTGCAGGAG 
GAGGAATGGG AAGTGGAGAG AGTGACTGAG CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGCATAG CTTTTGGAGA AAGTCAGAGT GAGGATGAAC AGTTTGAAAA TGACTTAGAG 
ACGGACCCAC CCAACTGGCA ACAGCTCGTC AGTCGAGAAG TGTTATTGGG ACTCAAGCCT 
TGTGAGATCA AAAGACAGGA AGTAATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACATTGA AGGTTCTAGA TCAAGTGTTT TATCAGCGAG TGTCCAGAGA AGGAATTTTG 
TCACCTTCAG AGCTACGGAA AATTTTTTCA AACTTAGAAG ATATTCTTCA ACTTCACATT 
GGATTGAATG AGCAAATGAA GGCTATTCGG AAGAGGAATG AGACCTCTGT TATTGATCAG 
ATTGGGGAAG ATTTGCTGAT CTGGTTCAGT GGACCCGGGG AGGAAAAGTT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCCTTT GCCCTGGAAA TGATCAAGTC TCGCCAGAAA 
AAGGATTCTC GATTCCAGAC TTTTGTGCAA GATGCTGAGA GTAATCCACT GTGTCGTCGT 
CTGCAGCTAA AGGATATCAT TCCCACTCAA ATGCAGAGGC TTACTAAGTA CCCTCTTCTC 
TTGGACAATA TTGCCAAATA CACAGAGTGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAC GTGAACCAAG CTGTTAAGGA GGCAGAGAAC 
AAACAGCGCT TGGAAGATTA TCAGCGTCGC CTTGATACCT CTAATCTGAA GTTGTCAGAA 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCACGAA 
GGCCCATTGG TTTGGAAGGT GAATAGAGAT AAAACAATTG ATCTGTATAC GTTGCTGCTG 
GAAGATATTC TTGTATTATT ACAAAAGCAG GATGATAGAC TGGTGTTAAG GTGTCACAGT 
AAGATTCTGG CATCTACAGC TGACAGCAAA CACACATTTA GCCCCGTCAT CAAGCTGAAT 
ACGGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT CTCCATGTCT 
GATAATGGAG CCCAAATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TATGTCGGAT GGCCGCGTCC GTGAAGGAGC AGTCCACAAA GCCGATCCCA 
CTGCCCCAGC CGCCACCGTG CGAAGGAGAC AACGATGAAG AAGAACCTCC AAAATTAAAA 
GTGGAACATC ATGACATTTC AGTCACTGGT CTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAGTCTCCTT TAATGTCATC AAAACCTCAG TCTCATTCGC TGGGTACCTC TGGGAAGTCG 
GAAGTAGATG ACCTCTTTGT GGCTGAGAGA CAGTTTGCAA AGGAGCAGCA TGCAGATGGG 
ACACTAAAGG AAGTTGGAGT GAATTATCAA ATCACAATCC CAGATCCACA CTTGCCTGTC 
TCGGAAGAAC GGTGGGCGCT GGATGCACTA AGGAATTTGG GTTTGTTGAA GCAGCTGCTG 
GTACACCAGC TGGGCTTGAC TGAGAAGAGC ACTCAGGAAG ACTGGCCACA TTTTCCAAGA 
TGCAGGACAG CCTCTCTGGG GGTGCAGGCA GACAGTGGAA CCCAGAACCC TGAAAATATT 
AAGGCCTGTC ATCCTGTTGA AGCACAGATG CCCTTTAGAA CTGGAACTGG TGACATTTCA 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTCTGCTC CACGGGATTC AGTAGTACTG 
GCATTCCAAG AGAGCCAGGC AAGTGACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTC CCTCAGAGCC AGAAGGGGGT CTTGATGAGA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGATAATCCA TCAGAAGGTG ATGGAGCAGT TAAAAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA ACCTATCTGA TCCTTGATGG CTATGACCCG 
GTGCAGGAGA GCTCCACAGA TGACGAGGTT GCTTCCTCGT TCCCCCTTCA GCCCGGGGCA 
GGCATCCCCT CTCTGGACTC TGGACACCAG CAGCAGCCTT CTCCCCAGAA TGCTCATTCT 
GATGGGGCTG TTTCACCGTT CAACCCAGAA TTACTGGTCC GGCAGCGCTG GGGAGCTATG 
GAGGATTCCT GTTTTGAGAT CCAGAGTCCC TCTGCTTGTG CTGATTCACA AAGCCAGATC 
ATGGAGTACA TTCGTAAAAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025122539.1
CDS	229..4554
Translation	MRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVTGHMSPIMTSPHSPGAPGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTSSKPLADGLAVGEGEADPGDGLGRLDYGSGDASRPGSDSADSPKSGLKERIYLEENPEKSEAIQDTDTQSLVGSPSTRVAPHIIGAEDDDFGSEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKQTNSKETRRVFLEFHQFFLDRSAHLKVSVPDEISVDLEKRRPELIPEDLHRHYIQTMQERVHPEVQKHLEDFRQKRSMGLTLAESELTKLDVERDKDRLTLEKERACAEQIVAKIEEVLMTAQAVEEEKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDREGEEKGKRRGFTSILGPPRRPSRHDNSAIGRAMELQKQRHPKHLSTPSSVSPEPQDSAKLRQSGSASEGADSGYLPANSTSSMVSGATLSQEGGKENDMGSKQAGETPASGDSLDGTPRTPNTIFDFPPPPLDQVQEEEWEVERVTEHGTPKPFRKFDSIAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAIRKRNETSVIDQIGEDLLIWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSNLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLNTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQPPPCEGDNDEEEPPKLKVEHHDISVTGLQSPDRDLGLESPLMSSKPQSHSLGTSGKSEVDDLFVAERQFAKEQHADGTLKEVGVNYQITIPDPHLPVSEERWALDALRNLGLLKQLLVHQLGLTEKSTQEDWPHFPRCRTASLGVQADSGTQNPENIKACHPVEAQMPFRTGTGDISTCYSPRTSTESSAPRDSVVLAFQESQASDILVMDHMIMTPEMPPSEPEGGLDESGEHFFDAREAHSDDNPSEGDGAVKKEEKDVNLRISGTYLILDGYDPVQESSTDDEVASSFPLQPGAGIPSLDSGHQQQPSPQNAHSDGAVSPFNPELLVRQRWGAMEDSCFEIQSPSACADSQSQIMEYIRKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_025266754.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025266754.1

ORF Insert Sequence:

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CATTCAAACC ATTTGGAAGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCCCCTGG GTCGCCCCAG ATTCCACTTG CTGATTCTGA AGTAGAGCCA 
TCGGTCACTG GACATATGTC TCCCATCATG ACATCCCCTC ATTCACCTGG TGCACCTGGG 
AATATGGAGA GAATTACTAG TCCTGTGCTC ATGGGGGAAG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT TCAGCTATTG 
CAGGAAGACT ACAACCGAAC ACCTGCTCAG AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CATCCTCCAA GCCGTTAGCT GACGGGCTAG CAGTGGGTGA AGGAGAAGCA 
GATCCTGGTG ATGGGCTAGG CAGGCTTGAC TATGGCAGCG GAGACGCTTC TCGGCCCGGT 
AGTGACAGTG CCGATAGTCC CAAGAGTGGC TTGAAAGAGA GGATTTATCT AGAGGAAAAC 
CCAGAAAAAA GTGAAGCAAT TCAGGACACT GACACTCAGT CGCTTGTTGG GAGTCCCTCT 
ACCCGTGTGG CACCTCATAT TATTGGAGCA GAGGACGATG ACTTTGGTAG TGAACATGAA 
CAGATCAATG GGCAGTGCAG CTGTTTTCAG AGCATTGAGC TGCTAAAATC TCGCCCTGCT 
CACTTGGCTG TCTTCTTACA CCATGTAGTT TCCCAGTTTG ACCCTGCAAC ATTGCTCTGT 
TACCTCTATT CAGACCTATA TAAACAGACC AATTCCAAGG AGACCCGTCG TGTCTTCCTT 
GAGTTCCATC AGTTCTTCCT GGATCGATCT GCTCACCTGA AGGTTTCTGT TCCTGATGAA 
ATATCAGTAG ATCTAGAAAA AAGACGACCA GAGCTTATTC CTGAGGATCT ACATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAC CCAGAAGTTC AGAAGCACTT GGAAGATTTT 
CGGCAGAAGC GCAGCATGGG GCTGACCCTG GCTGAAAGTG AACTGACTAA ACTTGATGTA 
GAGCGAGACA AGGACCGGTT AACTTTGGAG AAGGAACGGG CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTGTT GATGACTGCT CAGGCTGTAG AAGAAGAAAA GAGTTCAACA 
ATGCAATATG TTATTCTCAT GTACATGAAA CATTTGGGAG TAAAAGTGAA AGAACCTCGA 
AATTTGGAGC ACAAGCGAGG TCGGATTGGA TTCCTTCCAA AAATCAAGCA AAGTATGAAG 
AAAGATAGAG AAGGCGAAGA AAAGGGGAAG CGAAGAGGGT TCACCAGCAT CTTGGGACCC 
CCTAGGAGAC CAAGCCGCCA CGACAATAGC GCAATTGGCA GAGCCATGGA ACTGCAGAAG 
CAGCGCCACC CTAAGCACTT GTCCACACCC TCGTCTGTGA GTCCTGAACC TCAGGATTCT 
GCGAAGCTGC GCCAGAGTGG ATCAGCAAGT GAAGGAGCAG ACAGTGGATA CCTGCCAGCC 
AATTCCACGT CCTCCATGGT TTCAGGGGCC ACTCTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATATGGGAT CAAAACAAGC TGGAGAAACG CCAGCATCTG GAGACTCCTT GGATGGCACT 
CCTCGTACTC CCAACACCAT CTTTGATTTC CCACCTCCTC CATTAGACCA GGTGCAGGAG 
GAGGAATGGG AAGTGGAGAG AGTGACTGAG CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGCATAG CTTTTGGAGA AAGTCAGAGT GAGGATGAAC AGTTTGAAAA TGACTTAGAG 
ACGGACCCAC CCAACTGGCA ACAGCTCGTC AGTCGAGAAG TGTTATTGGG ACTCAAGCCT 
TGTGAGATCA AAAGACAGGA AGTAATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACATTGA AGGTTCTAGA TCAAGTGTTT TATCAGCGAG TGTCCAGAGA AGGAATTTTG 
TCACCTTCAG AGCTACGGAA AATTTTTTCA AACTTAGAAG ATATTCTTCA ACTTCACATT 
GGATTGAATG AGCAAATGAA GGCTATTCGG AAGAGGAATG AGACCTCTGT TATTGATCAG 
ATTGGGGAAG ATTTGCTGAT CTGGTTCAGT GGACCCGGGG AGGAAAAGTT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCCTTT GCCCTGGAAA TGATCAAGTC TCGCCAGAAA 
AAGGATTCTC GATTCCAGAC TTTTGTGCAA GATGCTGAGA GTAATCCACT GTGTCGTCGT 
CTGCAGCTAA AGGATATCAT TCCCACTCAA ATGCAGAGGC TTACTAAGTA CCCTCTTCTC 
TTGGACAATA TTGCCAAATA CACAGAGTGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAC GTGAACCAAG CTGTTAAGGA GGCAGAGAAC 
AAACAGCGCT TGGAAGATTA TCAGCGTCGC CTTGATACCT CTAATCTGAA GTTGTCAGAA 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCACGAA 
GGCCCATTGG TTTGGAAGGT GAATAGAGAT AAAACAATTG ATCTGTATAC GTTGCTGCTG 
GAAGATATTC TTGTATTATT ACAAAAGCAG GATGATAGAC TGGTGTTAAG GTGTCACAGT 
AAGATTCTGG CATCTACAGC TGACAGCAAA CACACATTTA GCCCCGTCAT CAAGCTGAAT 
ACGGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT CTCCATGTCT 
GATAATGGAG CCCAAATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TATGTCGGAT GGCCGCGTCC GTGAAGGAGC AGTCCACAAA GCCGATCCCA 
CTGCCCCAGC CGCCACCGTG CGAAGGAGAC AACGATGAAG AAGAACCTCC AAAATTAAAA 
GTGGAACATC ATGACATTTC AGTCACTGGT CTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAGTCTCCTT TAATGTCATC AAAACCTCAG TCTCATTCGC TGGGTACCTC TGGGAAGTCG 
GAAGTAGATG ACCTCTTTGT GGCTGAGAGA CAGTTTGCAA AGGAGCAGCA TGCAGATGGG 
ACACTAAAGG AAGTTGGAGT GAATTATCAA ATCACAATCC CAGATCCACA CTTGCCTGTC 
TCGGAAGAAC GGTGGGCGCT GGATGCACTA AGGAATTTGG GTTTGTTGAA GCAGCTGCTG 
GTACACCAGC TGGGCTTGAC TGAGAAGAGC ACTCAGGAAG ACTGGCCACA TTTTCCAAGA 
TGCAGGACAG CCTCTCTGGG GGTGCAGGCA GACAGTGGAA CCCAGAACCC TGAAAATATT 
AAGGCCTGTC ATCCTGTTGA AGCACAGATG CCCTTTAGAA CTGGAACTGG TGACATTTCA 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTCTGCTC CACGGGATTC AGTAGTACTG 
GCATTCCAAG AGAGCCAGGC AAGTGACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTC CCTCAGAGCC AGAAGGGGGT CTTGATGAGA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGATAATCCA TCAGAAGGTG ATGGAGCAGT TAAAAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA ACCTATCTGA TCCTTGATGG CTATGACCCG 
GTGCAGGAGA GCTCCACAGA TGACGAGGTT GCTTCCTCGT TCCCCCTTCA GCCCGGGGCA 
GGCATCCCCT CTCTGGACTC TGGACACCAG CAGCAGCCTT CTCCCCAGAA TGCTCATTCT 
GATGGGGCTG TTTCACCGTT CAACCCAGAA TTACTGGTCC GGCAGCGCTG GGGAGCTATG 
GAGGATTCCT GTTTTGAGAT CCAGAGTCCC TCTGCTTGTG CTGATTCACA AAGCCAGATC 
ATGGAGTACA TTCGTAAAAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_025266753.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025122538.1 Bubalus bubalis Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu50705
Clone ID Related Accession (Same CDS sequence)	XM_025266754.1 , XM_025266753.1
Accession Version	XM_025266753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	rho guanine nucleotide exchange factor 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CATTCAAACC ATTTGGAAGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCCCCTGG GTCGCCCCAG ATTCCACTTG CTGATTCTGA AGTAGAGCCA 
TCGGTCACTG GACATATGTC TCCCATCATG ACATCCCCTC ATTCACCTGG TGCACCTGGG 
AATATGGAGA GAATTACTAG TCCTGTGCTC ATGGGGGAAG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT TCAGCTATTG 
CAGGAAGACT ACAACCGAAC ACCTGCTCAG AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CATCCTCCAA GCCGTTAGCT GACGGGCTAG CAGTGGGTGA AGGAGAAGCA 
GATCCTGGTG ATGGGCTAGG CAGGCTTGAC TATGGCAGCG GAGACGCTTC TCGGCCCGGT 
AGTGACAGTG CCGATAGTCC CAAGAGTGGC TTGAAAGAGA GGATTTATCT AGAGGAAAAC 
CCAGAAAAAA GTGAAGCAAT TCAGGACACT GACACTCAGT CGCTTGTTGG GAGTCCCTCT 
ACCCGTGTGG CACCTCATAT TATTGGAGCA GAGGACGATG ACTTTGGTAG TGAACATGAA 
CAGATCAATG GGCAGTGCAG CTGTTTTCAG AGCATTGAGC TGCTAAAATC TCGCCCTGCT 
CACTTGGCTG TCTTCTTACA CCATGTAGTT TCCCAGTTTG ACCCTGCAAC ATTGCTCTGT 
TACCTCTATT CAGACCTATA TAAACAGACC AATTCCAAGG AGACCCGTCG TGTCTTCCTT 
GAGTTCCATC AGTTCTTCCT GGATCGATCT GCTCACCTGA AGGTTTCTGT TCCTGATGAA 
ATATCAGTAG ATCTAGAAAA AAGACGACCA GAGCTTATTC CTGAGGATCT ACATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAC CCAGAAGTTC AGAAGCACTT GGAAGATTTT 
CGGCAGAAGC GCAGCATGGG GCTGACCCTG GCTGAAAGTG AACTGACTAA ACTTGATGTA 
GAGCGAGACA AGGACCGGTT AACTTTGGAG AAGGAACGGG CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTGTT GATGACTGCT CAGGCTGTAG AAGAAGAAAA GAGTTCAACA 
ATGCAATATG TTATTCTCAT GTACATGAAA CATTTGGGAG TAAAAGTGAA AGAACCTCGA 
AATTTGGAGC ACAAGCGAGG TCGGATTGGA TTCCTTCCAA AAATCAAGCA AAGTATGAAG 
AAAGATAGAG AAGGCGAAGA AAAGGGGAAG CGAAGAGGGT TCACCAGCAT CTTGGGACCC 
CCTAGGAGAC CAAGCCGCCA CGACAATAGC GCAATTGGCA GAGCCATGGA ACTGCAGAAG 
CAGCGCCACC CTAAGCACTT GTCCACACCC TCGTCTGTGA GTCCTGAACC TCAGGATTCT 
GCGAAGCTGC GCCAGAGTGG ATCAGCAAGT GAAGGAGCAG ACAGTGGATA CCTGCCAGCC 
AATTCCACGT CCTCCATGGT TTCAGGGGCC ACTCTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATATGGGAT CAAAACAAGC TGGAGAAACG CCAGCATCTG GAGACTCCTT GGATGGCACT 
CCTCGTACTC CCAACACCAT CTTTGATTTC CCACCTCCTC CATTAGACCA GGTGCAGGAG 
GAGGAATGGG AAGTGGAGAG AGTGACTGAG CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGCATAG CTTTTGGAGA AAGTCAGAGT GAGGATGAAC AGTTTGAAAA TGACTTAGAG 
ACGGACCCAC CCAACTGGCA ACAGCTCGTC AGTCGAGAAG TGTTATTGGG ACTCAAGCCT 
TGTGAGATCA AAAGACAGGA AGTAATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACATTGA AGGTTCTAGA TCAAGTGTTT TATCAGCGAG TGTCCAGAGA AGGAATTTTG 
TCACCTTCAG AGCTACGGAA AATTTTTTCA AACTTAGAAG ATATTCTTCA ACTTCACATT 
GGATTGAATG AGCAAATGAA GGCTATTCGG AAGAGGAATG AGACCTCTGT TATTGATCAG 
ATTGGGGAAG ATTTGCTGAT CTGGTTCAGT GGACCCGGGG AGGAAAAGTT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCCTTT GCCCTGGAAA TGATCAAGTC TCGCCAGAAA 
AAGGATTCTC GATTCCAGAC TTTTGTGCAA GATGCTGAGA GTAATCCACT GTGTCGTCGT 
CTGCAGCTAA AGGATATCAT TCCCACTCAA ATGCAGAGGC TTACTAAGTA CCCTCTTCTC 
TTGGACAATA TTGCCAAATA CACAGAGTGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAC GTGAACCAAG CTGTTAAGGA GGCAGAGAAC 
AAACAGCGCT TGGAAGATTA TCAGCGTCGC CTTGATACCT CTAATCTGAA GTTGTCAGAA 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCACGAA 
GGCCCATTGG TTTGGAAGGT GAATAGAGAT AAAACAATTG ATCTGTATAC GTTGCTGCTG 
GAAGATATTC TTGTATTATT ACAAAAGCAG GATGATAGAC TGGTGTTAAG GTGTCACAGT 
AAGATTCTGG CATCTACAGC TGACAGCAAA CACACATTTA GCCCCGTCAT CAAGCTGAAT 
ACGGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT CTCCATGTCT 
GATAATGGAG CCCAAATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TATGTCGGAT GGCCGCGTCC GTGAAGGAGC AGTCCACAAA GCCGATCCCA 
CTGCCCCAGC CGCCACCGTG CGAAGGAGAC AACGATGAAG AAGAACCTCC AAAATTAAAA 
GTGGAACATC ATGACATTTC AGTCACTGGT CTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAGTCTCCTT TAATGTCATC AAAACCTCAG TCTCATTCGC TGGGTACCTC TGGGAAGTCG 
GAAGTAGATG ACCTCTTTGT GGCTGAGAGA CAGTTTGCAA AGGAGCAGCA TGCAGATGGG 
ACACTAAAGG AAGTTGGAGT GAATTATCAA ATCACAATCC CAGATCCACA CTTGCCTGTC 
TCGGAAGAAC GGTGGGCGCT GGATGCACTA AGGAATTTGG GTTTGTTGAA GCAGCTGCTG 
GTACACCAGC TGGGCTTGAC TGAGAAGAGC ACTCAGGAAG ACTGGCCACA TTTTCCAAGA 
TGCAGGACAG CCTCTCTGGG GGTGCAGGCA GACAGTGGAA CCCAGAACCC TGAAAATATT 
AAGGCCTGTC ATCCTGTTGA AGCACAGATG CCCTTTAGAA CTGGAACTGG TGACATTTCA 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTCTGCTC CACGGGATTC AGTAGTACTG 
GCATTCCAAG AGAGCCAGGC AAGTGACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTC CCTCAGAGCC AGAAGGGGGT CTTGATGAGA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGATAATCCA TCAGAAGGTG ATGGAGCAGT TAAAAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA ACCTATCTGA TCCTTGATGG CTATGACCCG 
GTGCAGGAGA GCTCCACAGA TGACGAGGTT GCTTCCTCGT TCCCCCTTCA GCCCGGGGCA 
GGCATCCCCT CTCTGGACTC TGGACACCAG CAGCAGCCTT CTCCCCAGAA TGCTCATTCT 
GATGGGGCTG TTTCACCGTT CAACCCAGAA TTACTGGTCC GGCAGCGCTG GGGAGCTATG 
GAGGATTCCT GTTTTGAGAT CCAGAGTCCC TCTGCTTGTG CTGATTCACA AAGCCAGATC 
ATGGAGTACA TTCGTAAAAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025122538.1
CDS	288..4613
Translation	MRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVTGHMSPIMTSPHSPGAPGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTSSKPLADGLAVGEGEADPGDGLGRLDYGSGDASRPGSDSADSPKSGLKERIYLEENPEKSEAIQDTDTQSLVGSPSTRVAPHIIGAEDDDFGSEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKQTNSKETRRVFLEFHQFFLDRSAHLKVSVPDEISVDLEKRRPELIPEDLHRHYIQTMQERVHPEVQKHLEDFRQKRSMGLTLAESELTKLDVERDKDRLTLEKERACAEQIVAKIEEVLMTAQAVEEEKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDREGEEKGKRRGFTSILGPPRRPSRHDNSAIGRAMELQKQRHPKHLSTPSSVSPEPQDSAKLRQSGSASEGADSGYLPANSTSSMVSGATLSQEGGKENDMGSKQAGETPASGDSLDGTPRTPNTIFDFPPPPLDQVQEEEWEVERVTEHGTPKPFRKFDSIAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAIRKRNETSVIDQIGEDLLIWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSNLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLNTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQPPPCEGDNDEEEPPKLKVEHHDISVTGLQSPDRDLGLESPLMSSKPQSHSLGTSGKSEVDDLFVAERQFAKEQHADGTLKEVGVNYQITIPDPHLPVSEERWALDALRNLGLLKQLLVHQLGLTEKSTQEDWPHFPRCRTASLGVQADSGTQNPENIKACHPVEAQMPFRTGTGDISTCYSPRTSTESSAPRDSVVLAFQESQASDILVMDHMIMTPEMPPSEPEGGLDESGEHFFDAREAHSDDNPSEGDGAVKKEEKDVNLRISGTYLILDGYDPVQESSTDDEVASSFPLQPGAGIPSLDSGHQQQPSPQNAHSDGAVSPFNPELLVRQRWGAMEDSCFEIQSPSACADSQSQIMEYIRKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_025266753.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025266753.1

ORF Insert Sequence:

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CATTCAAACC ATTTGGAAGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCCCCTGG GTCGCCCCAG ATTCCACTTG CTGATTCTGA AGTAGAGCCA 
TCGGTCACTG GACATATGTC TCCCATCATG ACATCCCCTC ATTCACCTGG TGCACCTGGG 
AATATGGAGA GAATTACTAG TCCTGTGCTC ATGGGGGAAG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT TCAGCTATTG 
CAGGAAGACT ACAACCGAAC ACCTGCTCAG AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CATCCTCCAA GCCGTTAGCT GACGGGCTAG CAGTGGGTGA AGGAGAAGCA 
GATCCTGGTG ATGGGCTAGG CAGGCTTGAC TATGGCAGCG GAGACGCTTC TCGGCCCGGT 
AGTGACAGTG CCGATAGTCC CAAGAGTGGC TTGAAAGAGA GGATTTATCT AGAGGAAAAC 
CCAGAAAAAA GTGAAGCAAT TCAGGACACT GACACTCAGT CGCTTGTTGG GAGTCCCTCT 
ACCCGTGTGG CACCTCATAT TATTGGAGCA GAGGACGATG ACTTTGGTAG TGAACATGAA 
CAGATCAATG GGCAGTGCAG CTGTTTTCAG AGCATTGAGC TGCTAAAATC TCGCCCTGCT 
CACTTGGCTG TCTTCTTACA CCATGTAGTT TCCCAGTTTG ACCCTGCAAC ATTGCTCTGT 
TACCTCTATT CAGACCTATA TAAACAGACC AATTCCAAGG AGACCCGTCG TGTCTTCCTT 
GAGTTCCATC AGTTCTTCCT GGATCGATCT GCTCACCTGA AGGTTTCTGT TCCTGATGAA 
ATATCAGTAG ATCTAGAAAA AAGACGACCA GAGCTTATTC CTGAGGATCT ACATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAC CCAGAAGTTC AGAAGCACTT GGAAGATTTT 
CGGCAGAAGC GCAGCATGGG GCTGACCCTG GCTGAAAGTG AACTGACTAA ACTTGATGTA 
GAGCGAGACA AGGACCGGTT AACTTTGGAG AAGGAACGGG CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTGTT GATGACTGCT CAGGCTGTAG AAGAAGAAAA GAGTTCAACA 
ATGCAATATG TTATTCTCAT GTACATGAAA CATTTGGGAG TAAAAGTGAA AGAACCTCGA 
AATTTGGAGC ACAAGCGAGG TCGGATTGGA TTCCTTCCAA AAATCAAGCA AAGTATGAAG 
AAAGATAGAG AAGGCGAAGA AAAGGGGAAG CGAAGAGGGT TCACCAGCAT CTTGGGACCC 
CCTAGGAGAC CAAGCCGCCA CGACAATAGC GCAATTGGCA GAGCCATGGA ACTGCAGAAG 
CAGCGCCACC CTAAGCACTT GTCCACACCC TCGTCTGTGA GTCCTGAACC TCAGGATTCT 
GCGAAGCTGC GCCAGAGTGG ATCAGCAAGT GAAGGAGCAG ACAGTGGATA CCTGCCAGCC 
AATTCCACGT CCTCCATGGT TTCAGGGGCC ACTCTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATATGGGAT CAAAACAAGC TGGAGAAACG CCAGCATCTG GAGACTCCTT GGATGGCACT 
CCTCGTACTC CCAACACCAT CTTTGATTTC CCACCTCCTC CATTAGACCA GGTGCAGGAG 
GAGGAATGGG AAGTGGAGAG AGTGACTGAG CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGCATAG CTTTTGGAGA AAGTCAGAGT GAGGATGAAC AGTTTGAAAA TGACTTAGAG 
ACGGACCCAC CCAACTGGCA ACAGCTCGTC AGTCGAGAAG TGTTATTGGG ACTCAAGCCT 
TGTGAGATCA AAAGACAGGA AGTAATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACATTGA AGGTTCTAGA TCAAGTGTTT TATCAGCGAG TGTCCAGAGA AGGAATTTTG 
TCACCTTCAG AGCTACGGAA AATTTTTTCA AACTTAGAAG ATATTCTTCA ACTTCACATT 
GGATTGAATG AGCAAATGAA GGCTATTCGG AAGAGGAATG AGACCTCTGT TATTGATCAG 
ATTGGGGAAG ATTTGCTGAT CTGGTTCAGT GGACCCGGGG AGGAAAAGTT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCCTTT GCCCTGGAAA TGATCAAGTC TCGCCAGAAA 
AAGGATTCTC GATTCCAGAC TTTTGTGCAA GATGCTGAGA GTAATCCACT GTGTCGTCGT 
CTGCAGCTAA AGGATATCAT TCCCACTCAA ATGCAGAGGC TTACTAAGTA CCCTCTTCTC 
TTGGACAATA TTGCCAAATA CACAGAGTGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAC GTGAACCAAG CTGTTAAGGA GGCAGAGAAC 
AAACAGCGCT TGGAAGATTA TCAGCGTCGC CTTGATACCT CTAATCTGAA GTTGTCAGAA 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCACGAA 
GGCCCATTGG TTTGGAAGGT GAATAGAGAT AAAACAATTG ATCTGTATAC GTTGCTGCTG 
GAAGATATTC TTGTATTATT ACAAAAGCAG GATGATAGAC TGGTGTTAAG GTGTCACAGT 
AAGATTCTGG CATCTACAGC TGACAGCAAA CACACATTTA GCCCCGTCAT CAAGCTGAAT 
ACGGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT CTCCATGTCT 
GATAATGGAG CCCAAATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TATGTCGGAT GGCCGCGTCC GTGAAGGAGC AGTCCACAAA GCCGATCCCA 
CTGCCCCAGC CGCCACCGTG CGAAGGAGAC AACGATGAAG AAGAACCTCC AAAATTAAAA 
GTGGAACATC ATGACATTTC AGTCACTGGT CTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAGTCTCCTT TAATGTCATC AAAACCTCAG TCTCATTCGC TGGGTACCTC TGGGAAGTCG 
GAAGTAGATG ACCTCTTTGT GGCTGAGAGA CAGTTTGCAA AGGAGCAGCA TGCAGATGGG 
ACACTAAAGG AAGTTGGAGT GAATTATCAA ATCACAATCC CAGATCCACA CTTGCCTGTC 
TCGGAAGAAC GGTGGGCGCT GGATGCACTA AGGAATTTGG GTTTGTTGAA GCAGCTGCTG 
GTACACCAGC TGGGCTTGAC TGAGAAGAGC ACTCAGGAAG ACTGGCCACA TTTTCCAAGA 
TGCAGGACAG CCTCTCTGGG GGTGCAGGCA GACAGTGGAA CCCAGAACCC TGAAAATATT 
AAGGCCTGTC ATCCTGTTGA AGCACAGATG CCCTTTAGAA CTGGAACTGG TGACATTTCA 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTCTGCTC CACGGGATTC AGTAGTACTG 
GCATTCCAAG AGAGCCAGGC AAGTGACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTC CCTCAGAGCC AGAAGGGGGT CTTGATGAGA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGATAATCCA TCAGAAGGTG ATGGAGCAGT TAAAAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA ACCTATCTGA TCCTTGATGG CTATGACCCG 
GTGCAGGAGA GCTCCACAGA TGACGAGGTT GCTTCCTCGT TCCCCCTTCA GCCCGGGGCA 
GGCATCCCCT CTCTGGACTC TGGACACCAG CAGCAGCCTT CTCCCCAGAA TGCTCATTCT 
GATGGGGCTG TTTCACCGTT CAACCCAGAA TTACTGGTCC GGCAGCGCTG GGGAGCTATG 
GAGGATTCCT GTTTTGAGAT CCAGAGTCCC TCTGCTTGTG CTGATTCACA AAGCCAGATC 
ATGGAGTACA TTCGTAAAAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_006044891.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044953.1 Bubalus bubalis Rho guanine nucleotide exchange factor (GEF) 12 (ARHGEF12), mRNA.

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CloneID	OBu03996
Clone ID Related Accession (Same CDS sequence)	XM_006044891.1
Accession Version	XM_006044891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	LOW QUALITY PROTEIN: rho guanine nucleotide exchange factor 12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783669.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGTGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTACA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AAATATATGG TCTTGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTGTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCCCCTGGG TCGCCCCAGA TTCCACTTGC TGATTCTGAA 
GTAGAGCCAT CGGTCACTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGT 
GCACCTGGGA ATATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAAGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTT 
CAGCTATTGC AGGAAGACTA CAACCGAACA CCTGCTCAGA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC ATCCTCCAAG CCGTTAGCTG ACGGGCTAGC AGTGGGTGAA 
GGAGAAGCAG ATCCTGGTGA TGGGCTAGGC AGGCTTGACT ATGGCAGCGG AGACGCTTCT 
CGGCCCGGTA GTGACAGTGC CGATAGTCCC AAGAGTGGCT TGAAAGAGAG GATTTATCTA 
GAGGAAAACC CAGAAAAAAG TGAAGCAATT CAGGACACTG ACACTCAGTC GCTTGTTGGG 
AGTCCCTCTA CCCGTGTGGC ACCTCATATT ATTNNAGCAG AGGACGATGA CTTTGGTAGT 
GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTTCAGA GCATTGAGCT GCTAAAATCT 
CGCCCTGCTC ACTTGGCTGT CTTCTTACAC CATGTAGTTT CCCAGTTTGA CCCTGCAACA 
TTGCTCTGTT ACCTCTATTC AGACCTATAT AAACAGACCA ATTCCAAGGA GACCCGTCGT 
GTCTTCCTTG AGTTCCATCA GTTCTTCCTG GATCGATCTG CTCACCTGAA GGTTTCTGTT 
CCTGATGAAA TATCAGTAGA TCTAGAAAAA AGACGACCAG AGCTTATTCC TGAGGATCTA 
CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG 
GAAGATTTTC GGCAGAAGCG CAGCATGGGG CTGACCCTGG CTGAAAGTGA ACTGACTAAA 
CTTGATGTAG AGCGAGACAA GGACCGGTTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA 
CAGATTGTTG CCAAAATTGA AGAAGTGTTG ATGACTGCTC AGGCTGTAGA AGAAGAAAAG 
AGTTCAACAA TGCAATATGT TATTCTCATG TACATGAAAC ATTTGGGAGT AAAAGTGAAA 
GAACCTCGAA ATTTGGAGCA CAAGCGAGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAA 
AGTATGAAGA AAGATAGAGA AGGCGAAGAA AAGGGGAAGC GAAGAGGGTT CACCAGCATC 
TTGGGACCCC CTAGGAGACC AAGCCGCCAC GACAATAGCG CAATTGGCAG AGCCATGGAA 
CTGCAGAAGC AGCGCCACCC TAAGCACTTG TCCACACCCT CGTCTGTGAG TCCTGAACCT 
CAGGATTCTG CGAAGCTGCG CCAGAGTGGA TCAGCAAGTG AAGGAGCAGA CAGTGGATAC 
CTGCCAGCCA ATTCCACGTC CTCCATGGTT TCAGGGGCCA CTCTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATATGGGATC AAAACAAGCT GGAGAAACGC CAGCATCTGG AGACTCCTTG 
GATGGCACTC CTCGTACTCC CAACACCATC TTTGATTTCC CACCTCCTCC ATTAGACCAG 
GTGCAGGAGG AGGAATGGGA AGTGGAGAGA GTGACTGAGC ATGGGACACC AAAGCCCTTT 
CGAAAGTTTG ACAGCATAGC TTTTGGAGAA AGTCAGAGTG AGGATGAACA GTTTGAAAAT 
GACTTAGAGA CGGACCCACC CAACTGGCAA CAGCTCGTCA GTCGAGAAGT GTTATTGGGA 
CTCAAGCCTT GTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACATTGAA GGTTCTAGAT CAAGTGTTTT ATCAGCGAGT GTCCAGAGAA 
GGAATTTTGT CACCTTCAGA GCTACGGAAA ATTTTTTCAA ACTTAGAAGA TATTCTTCAA 
CTTCACATTG GATTGAATGA GCAAATGAAG GCTATTCGGA AGAGGAATGA GACCTCTGTT 
ATTGATCAGA TTGGGGAAGA TTTGCTGATC TGGTTCAGTG GACCCGGGGA GGAAAAGTTG 
AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCCTTTG CCCTGGAAAT GATCAAGTCT 
CGCCAGAAAA AGGATTCTCG ATTCCAGACT TTTGTGCAAG ATGCTGAGAG TAATCCACTG 
TGTCGTCGTC TGCAGCTAAA GGATATCATT CCCACTCAAA TGCAGAGGCT TACTAAGTAC 
CCTCTTCTCT TGGACAATAT TGCCAAATAC ACAGAGTGGC CAACAGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTACG TGAACCAAGC TGTTAAGGAG 
GCAGAGAACA AACAGCGCTT GGAAGATTAT CAGCGTCGCC TTGATACCTC TAATCTGAAG 
TTGTCAGAAT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCACGAAG GCCCATTGGT TTGGAAGGTG AATAGAGATA AAACAATTGA TCTGTATACG 
TTGCTGCTGG AAGATATTCT TGTATTATTA CAAAAGCAGG ATGATAGACT GGTGTTAAGG 
TGTCACAGTA AGATTCTGGC ATCTACAGCT GACAGCAAAC ACACATTTAG CCCTGTCATC 
AAGCTGAATA CGGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATC 
TCCATGTCTG ATAATGGAGC CCAAATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTAAT ATGTCGGATG GCCGCGTCCG TGAAGGAGCA GTCCACAAAG 
CCGATCCCAC TGCCCCAGCC GCCACCGTGC GAAGGAGACA ACGATGAAGA AGAACCTCCA 
AAATTAAAAG TGGAACATCA TGACATTTCA GTCACTGGTC TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AGTCTCCTTT AATGTCATCA AAACCTCAGT CTCATTCGCT GGGTACCTCT 
GGGAAGTCGG AAGTAGATGA CCTCTTTGTG GCTGAGAGAC AGTTTGCAAA GGAGCAGCAT 
GCAGATGGGA CACTAAAGGA AGTTGGAGTG AATTATCAAA TCACAATCCC AGATCCACAC 
TTGCCTGTCG CGGAAGAACG GTGGGCGCTG GATGCACTAA GGAATTTGGG TTTGTTGAAG 
CAGCTGCTGG TACACCAGCT GGGCTTGACT GAGAAGAGCA CTCAGGAAGA CTGGCCACAT 
TTTCCAAGAT GCAGGACAGC CTCTCTGGGG GTGCAGGCAG ACAGTGGAAC CCAGAACCCT 
GAAAATATTA AGGCCTGTCA TCCTGTTGAA GCACAGATGC CCTTTAGAAC TGGAACTGGT 
GACATTTCAA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTCTGCTCC ACGGGATTCA 
GTAGTACTGG CATTCCAAGA GAGCCAGGCA AGTGACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTCC CTCAGAGCCA GAAGGGGGTC TTGATGAGAG TGGAGAGCAC 
TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT 
AAAAAGGAAG AGAAGGATGT TAATTTGCGC ATCTCAGGAA CCTATCTGAT CCTTGATGGC 
TATGACCCGG TGCAGGAGAG CTCCACAGAT GACGAGGTTG CTTCCTCGTT CCCCCTTCAG 
CCCGGGGCAG GCATCCCCTC TCTGGACTCT GGACACCAGC AGCAGCCTTC TCCCCAGAAT 
GCTCATTCTG ATGGGGCTGT TTCACCGTTC AACCCAGAAT TACTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGGATTCCTG TTTTGAGATC CAGAGTCCCT CTGCTTGTGC TGATTCACAA 
AGCCAGATCA TGGAGTACAT TCGTAAAATA GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044953.1
CDS	319..4953
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERSTSHDFDPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVTGHMSPIMTSPHSPGAPGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTSSKPLADGLAVGEGEADPGDGLGRLDYGSGDASRPGSDSADSPKSGLKERIYLEENPEKSEAIQDTDTQSLVGSPSTRVAPHIInullAEDDDFGSEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKQTNSKETRRVFLEFHQFFLDRSAHLKVSVPDEISVDLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDVERDKDRLTLEKERACAEQIVAKIEEVLMTAQAVEEEKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDREGEEKGKRRGFTSILGPPRRPSRHDNSAIGRAMELQKQRHPKHLSTPSSVSPEPQDSAKLRQSGSASEGADSGYLPANSTSSMVSGATLSQEGGKENDMGSKQAGETPASGDSLDGTPRTPNTIFDFPPPPLDQVQEEEWEVERVTEHGTPKPFRKFDSIAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAIRKRNETSVIDQIGEDLLIWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSNLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLNTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQPPPCEGDNDEEEPPKLKVEHHDISVTGLQSPDRDLGLESPLMSSKPQSHSLGTSGKSEVDDLFVAERQFAKEQHADGTLKEVGVNYQITIPDPHLPVAEERWALDALRNLGLLKQLLVHQLGLTEKSTQEDWPHFPRCRTASLGVQADSGTQNPENIKACHPVEAQMPFRTGTGDISTCYSPRTSTESSAPRDSVVLAFQESQASDILVMDHMIMTPEMPPSEPEGGLDESGEHFFDAREAHSDDNPSEGDGAVKKEEKDVNLRISGTYLILDGYDPVQESSTDDEVASSFPLQPGAGIPSLDSGHQQQPSPQNAHSDGAVSPFNPELLVQQRWGAMEDSCFEIQSPSACADSQSQIMEYIRKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_006044891.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis Rho guanine nucleotide exchange factor (GEF) 12 (ARHGEF12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044891.1

ORF Insert Sequence:

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGTGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTACA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AAATATATGG TCTTGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTGTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCCCCTGGG TCGCCCCAGA TTCCACTTGC TGATTCTGAA 
GTAGAGCCAT CGGTCACTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGT 
GCACCTGGGA ATATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAAGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTT 
CAGCTATTGC AGGAAGACTA CAACCGAACA CCTGCTCAGA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC ATCCTCCAAG CCGTTAGCTG ACGGGCTAGC AGTGGGTGAA 
GGAGAAGCAG ATCCTGGTGA TGGGCTAGGC AGGCTTGACT ATGGCAGCGG AGACGCTTCT 
CGGCCCGGTA GTGACAGTGC CGATAGTCCC AAGAGTGGCT TGAAAGAGAG GATTTATCTA 
GAGGAAAACC CAGAAAAAAG TGAAGCAATT CAGGACACTG ACACTCAGTC GCTTGTTGGG 
AGTCCCTCTA CCCGTGTGGC ACCTCATATT ATTNNAGCAG AGGACGATGA CTTTGGTAGT 
GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTTCAGA GCATTGAGCT GCTAAAATCT 
CGCCCTGCTC ACTTGGCTGT CTTCTTACAC CATGTAGTTT CCCAGTTTGA CCCTGCAACA 
TTGCTCTGTT ACCTCTATTC AGACCTATAT AAACAGACCA ATTCCAAGGA GACCCGTCGT 
GTCTTCCTTG AGTTCCATCA GTTCTTCCTG GATCGATCTG CTCACCTGAA GGTTTCTGTT 
CCTGATGAAA TATCAGTAGA TCTAGAAAAA AGACGACCAG AGCTTATTCC TGAGGATCTA 
CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG 
GAAGATTTTC GGCAGAAGCG CAGCATGGGG CTGACCCTGG CTGAAAGTGA ACTGACTAAA 
CTTGATGTAG AGCGAGACAA GGACCGGTTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA 
CAGATTGTTG CCAAAATTGA AGAAGTGTTG ATGACTGCTC AGGCTGTAGA AGAAGAAAAG 
AGTTCAACAA TGCAATATGT TATTCTCATG TACATGAAAC ATTTGGGAGT AAAAGTGAAA 
GAACCTCGAA ATTTGGAGCA CAAGCGAGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAA 
AGTATGAAGA AAGATAGAGA AGGCGAAGAA AAGGGGAAGC GAAGAGGGTT CACCAGCATC 
TTGGGACCCC CTAGGAGACC AAGCCGCCAC GACAATAGCG CAATTGGCAG AGCCATGGAA 
CTGCAGAAGC AGCGCCACCC TAAGCACTTG TCCACACCCT CGTCTGTGAG TCCTGAACCT 
CAGGATTCTG CGAAGCTGCG CCAGAGTGGA TCAGCAAGTG AAGGAGCAGA CAGTGGATAC 
CTGCCAGCCA ATTCCACGTC CTCCATGGTT TCAGGGGCCA CTCTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATATGGGATC AAAACAAGCT GGAGAAACGC CAGCATCTGG AGACTCCTTG 
GATGGCACTC CTCGTACTCC CAACACCATC TTTGATTTCC CACCTCCTCC ATTAGACCAG 
GTGCAGGAGG AGGAATGGGA AGTGGAGAGA GTGACTGAGC ATGGGACACC AAAGCCCTTT 
CGAAAGTTTG ACAGCATAGC TTTTGGAGAA AGTCAGAGTG AGGATGAACA GTTTGAAAAT 
GACTTAGAGA CGGACCCACC CAACTGGCAA CAGCTCGTCA GTCGAGAAGT GTTATTGGGA 
CTCAAGCCTT GTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACATTGAA GGTTCTAGAT CAAGTGTTTT ATCAGCGAGT GTCCAGAGAA 
GGAATTTTGT CACCTTCAGA GCTACGGAAA ATTTTTTCAA ACTTAGAAGA TATTCTTCAA 
CTTCACATTG GATTGAATGA GCAAATGAAG GCTATTCGGA AGAGGAATGA GACCTCTGTT 
ATTGATCAGA TTGGGGAAGA TTTGCTGATC TGGTTCAGTG GACCCGGGGA GGAAAAGTTG 
AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCCTTTG CCCTGGAAAT GATCAAGTCT 
CGCCAGAAAA AGGATTCTCG ATTCCAGACT TTTGTGCAAG ATGCTGAGAG TAATCCACTG 
TGTCGTCGTC TGCAGCTAAA GGATATCATT CCCACTCAAA TGCAGAGGCT TACTAAGTAC 
CCTCTTCTCT TGGACAATAT TGCCAAATAC ACAGAGTGGC CAACAGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTACG TGAACCAAGC TGTTAAGGAG 
GCAGAGAACA AACAGCGCTT GGAAGATTAT CAGCGTCGCC TTGATACCTC TAATCTGAAG 
TTGTCAGAAT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCACGAAG GCCCATTGGT TTGGAAGGTG AATAGAGATA AAACAATTGA TCTGTATACG 
TTGCTGCTGG AAGATATTCT TGTATTATTA CAAAAGCAGG ATGATAGACT GGTGTTAAGG 
TGTCACAGTA AGATTCTGGC ATCTACAGCT GACAGCAAAC ACACATTTAG CCCTGTCATC 
AAGCTGAATA CGGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATC 
TCCATGTCTG ATAATGGAGC CCAAATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTAAT ATGTCGGATG GCCGCGTCCG TGAAGGAGCA GTCCACAAAG 
CCGATCCCAC TGCCCCAGCC GCCACCGTGC GAAGGAGACA ACGATGAAGA AGAACCTCCA 
AAATTAAAAG TGGAACATCA TGACATTTCA GTCACTGGTC TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AGTCTCCTTT AATGTCATCA AAACCTCAGT CTCATTCGCT GGGTACCTCT 
GGGAAGTCGG AAGTAGATGA CCTCTTTGTG GCTGAGAGAC AGTTTGCAAA GGAGCAGCAT 
GCAGATGGGA CACTAAAGGA AGTTGGAGTG AATTATCAAA TCACAATCCC AGATCCACAC 
TTGCCTGTCG CGGAAGAACG GTGGGCGCTG GATGCACTAA GGAATTTGGG TTTGTTGAAG 
CAGCTGCTGG TACACCAGCT GGGCTTGACT GAGAAGAGCA CTCAGGAAGA CTGGCCACAT 
TTTCCAAGAT GCAGGACAGC CTCTCTGGGG GTGCAGGCAG ACAGTGGAAC CCAGAACCCT 
GAAAATATTA AGGCCTGTCA TCCTGTTGAA GCACAGATGC CCTTTAGAAC TGGAACTGGT 
GACATTTCAA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTCTGCTCC ACGGGATTCA 
GTAGTACTGG CATTCCAAGA GAGCCAGGCA AGTGACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTCC CTCAGAGCCA GAAGGGGGTC TTGATGAGAG TGGAGAGCAC 
TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT 
AAAAAGGAAG AGAAGGATGT TAATTTGCGC ATCTCAGGAA CCTATCTGAT CCTTGATGGC 
TATGACCCGG TGCAGGAGAG CTCCACAGAT GACGAGGTTG CTTCCTCGTT CCCCCTTCAG 
CCCGGGGCAG GCATCCCCTC TCTGGACTCT GGACACCAGC AGCAGCCTTC TCCCCAGAAT 
GCTCATTCTG ATGGGGCTGT TTCACCGTTC AACCCAGAAT TACTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGGATTCCTG TTTTGAGATC CAGAGTCCCT CTGCTTGTGC TGATTCACAA 
AGCCAGATCA TGGAGTACAT TCGTAAAATA GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_006044891.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044953.2 Bubalus bubalis Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.

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CloneID	OBu50702
Clone ID Related Accession (Same CDS sequence)	XM_006044891.2
Accession Version	XM_006044891.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	rho guanine nucleotide exchange factor 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006044891.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGTGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTACA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AAATATATGG TCTTGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTGTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCCCCTGGG TCGCCCCAGA TTCCACTTGC TGATTCTGAA 
GTAGAGCCAT CGGTCACTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGT 
GCACCTGGGA ATATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAAGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTT 
CAGCTATTGC AGGAAGACTA CAACCGAACA CCTGCTCAGA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC ATCCTCCAAG CCGTTAGCTG ACGGGCTAGC AGTGGGTGAA 
GGAGAAGCAG ATCCTGGTGA TGGGCTAGGC AGGCTTGACT ATGGCAGCGG AGACGCTTCT 
CGGCCCGGTA GTGACAGTGC CGATAGTCCC AAGAGTGGCT TGAAAGAGAG GATTTATCTA 
GAGGAAAACC CAGAAAAAAG TGAAGCAATT CAGGACACTG ACACTCAGTC GCTTGTTGGG 
AGTCCCTCTA CCCGTGTGGC ACCTCATATT ATTGGAGCAG AGGACGATGA CTTTGGTAGT 
GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTTCAGA GCATTGAGCT GCTAAAATCT 
CGCCCTGCTC ACTTGGCTGT CTTCTTACAC CATGTAGTTT CCCAGTTTGA CCCTGCAACA 
TTGCTCTGTT ACCTCTATTC AGACCTATAT AAACAGACCA ATTCCAAGGA GACCCGTCGT 
GTCTTCCTTG AGTTCCATCA GTTCTTCCTG GATCGATCTG CTCACCTGAA GGTTTCTGTT 
CCTGATGAAA TATCAGTAGA TCTAGAAAAA AGACGACCAG AGCTTATTCC TGAGGATCTA 
CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCACC CAGAAGTTCA GAAGCACTTG 
GAAGATTTTC GGCAGAAGCG CAGCATGGGG CTGACCCTGG CTGAAAGTGA ACTGACTAAA 
CTTGATGTAG AGCGAGACAA GGACCGGTTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA 
CAGATTGTTG CCAAAATTGA AGAAGTGTTG ATGACTGCTC AGGCTGTAGA AGAAGAAAAG 
AGTTCAACAA TGCAATATGT TATTCTCATG TACATGAAAC ATTTGGGAGT AAAAGTGAAA 
GAACCTCGAA ATTTGGAGCA CAAGCGAGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAA 
AGTATGAAGA AAGATAGAGA AGGCGAAGAA AAGGGGAAGC GAAGAGGGTT CACCAGCATC 
TTGGGACCCC CTAGGAGACC AAGCCGCCAC GACAATAGCG CAATTGGCAG AGCCATGGAA 
CTGCAGAAGC AGCGCCACCC TAAGCACTTG TCCACACCCT CGTCTGTGAG TCCTGAACCT 
CAGGATTCTG CGAAGCTGCG CCAGAGTGGA TCAGCAAGTG AAGGAGCAGA CAGTGGATAC 
CTGCCAGCCA ATTCCACGTC CTCCATGGTT TCAGGGGCCA CTCTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATATGGGATC AAAACAAGCT GGAGAAACGC CAGCATCTGG AGACTCCTTG 
GATGGCACTC CTCGTACTCC CAACACCATC TTTGATTTCC CACCTCCTCC ATTAGACCAG 
GTGCAGGAGG AGGAATGGGA AGTGGAGAGA GTGACTGAGC ATGGGACACC AAAGCCCTTT 
CGAAAGTTTG ACAGCATAGC TTTTGGAGAA AGTCAGAGTG AGGATGAACA GTTTGAAAAT 
GACTTAGAGA CGGACCCACC CAACTGGCAA CAGCTCGTCA GTCGAGAAGT GTTATTGGGA 
CTCAAGCCTT GTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACATTGAA GGTTCTAGAT CAAGTGTTTT ATCAGCGAGT GTCCAGAGAA 
GGAATTTTGT CACCTTCAGA GCTACGGAAA ATTTTTTCAA ACTTAGAAGA TATTCTTCAA 
CTTCACATTG GATTGAATGA GCAAATGAAG GCTATTCGGA AGAGGAATGA GACCTCTGTT 
ATTGATCAGA TTGGGGAAGA TTTGCTGATC TGGTTCAGTG GACCCGGGGA GGAAAAGTTG 
AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCCTTTG CCCTGGAAAT GATCAAGTCT 
CGCCAGAAAA AGGATTCTCG ATTCCAGACT TTTGTGCAAG ATGCTGAGAG TAATCCACTG 
TGTCGTCGTC TGCAGCTAAA GGATATCATT CCCACTCAAA TGCAGAGGCT TACTAAGTAC 
CCTCTTCTCT TGGACAATAT TGCCAAATAC ACAGAGTGGC CAACAGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTACG TGAACCAAGC TGTTAAGGAG 
GCAGAGAACA AACAGCGCTT GGAAGATTAT CAGCGTCGCC TTGATACCTC TAATCTGAAG 
TTGTCAGAAT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCACGAAG GCCCATTGGT TTGGAAGGTG AATAGAGATA AAACAATTGA TCTGTATACG 
TTGCTGCTGG AAGATATTCT TGTATTATTA CAAAAGCAGG ATGATAGACT GGTGTTAAGG 
TGTCACAGTA AGATTCTGGC ATCTACAGCT GACAGCAAAC ACACATTTAG CCCCGTCATC 
AAGCTGAATA CGGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATC 
TCCATGTCTG ATAATGGAGC CCAAATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTAAT ATGTCGGATG GCCGCGTCCG TGAAGGAGCA GTCCACAAAG 
CCGATCCCAC TGCCCCAGCC GCCACCGTGC GAAGGAGACA ACGATGAAGA AGAACCTCCA 
AAATTAAAAG TGGAACATCA TGACATTTCA GTCACTGGTC TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AGTCTCCTTT AATGTCATCA AAACCTCAGT CTCATTCGCT GGGTACCTCT 
GGGAAGTCGG AAGTAGATGA CCTCTTTGTG GCTGAGAGAC AGTTTGCAAA GGAGCAGCAT 
GCAGATGGGA CACTAAAGGA AGTTGGAGTG AATTATCAAA TCACAATCCC AGATCCACAC 
TTGCCTGTCT CGGAAGAACG GTGGGCGCTG GATGCACTAA GGAATTTGGG TTTGTTGAAG 
CAGCTGCTGG TACACCAGCT GGGCTTGACT GAGAAGAGCA CTCAGGAAGA CTGGCCACAT 
TTTCCAAGAT GCAGGACAGC CTCTCTGGGG GTGCAGGCAG ACAGTGGAAC CCAGAACCCT 
GAAAATATTA AGGCCTGTCA TCCTGTTGAA GCACAGATGC CCTTTAGAAC TGGAACTGGT 
GACATTTCAA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTCTGCTCC ACGGGATTCA 
GTAGTACTGG CATTCCAAGA GAGCCAGGCA AGTGACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTCC CTCAGAGCCA GAAGGGGGTC TTGATGAGAG TGGAGAGCAC 
TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT 
AAAAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA CCTATCTGAT CCTTGATGGC 
TATGACCCGG TGCAGGAGAG CTCCACAGAT GACGAGGTTG CTTCCTCGTT CCCCCTTCAG 
CCCGGGGCAG GCATCCCCTC TCTGGACTCT GGACACCAGC AGCAGCCTTC TCCCCAGAAT 
GCTCATTCTG ATGGGGCTGT TTCACCGTTC AACCCAGAAT TACTGGTCCG GCAGCGCTGG 
GGAGCTATGG AGGATTCCTG TTTTGAGATC CAGAGTCCCT CTGCTTGTGC TGATTCACAA 
AGCCAGATCA TGGAGTACAT TCGTAAAATA GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044953.2
CDS	166..4800
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERSTSHDFDPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVTGHMSPIMTSPHSPGAPGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTSSKPLADGLAVGEGEADPGDGLGRLDYGSGDASRPGSDSADSPKSGLKERIYLEENPEKSEAIQDTDTQSLVGSPSTRVAPHIIGAEDDDFGSEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKQTNSKETRRVFLEFHQFFLDRSAHLKVSVPDEISVDLEKRRPELIPEDLHRHYIQTMQERVHPEVQKHLEDFRQKRSMGLTLAESELTKLDVERDKDRLTLEKERACAEQIVAKIEEVLMTAQAVEEEKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDREGEEKGKRRGFTSILGPPRRPSRHDNSAIGRAMELQKQRHPKHLSTPSSVSPEPQDSAKLRQSGSASEGADSGYLPANSTSSMVSGATLSQEGGKENDMGSKQAGETPASGDSLDGTPRTPNTIFDFPPPPLDQVQEEEWEVERVTEHGTPKPFRKFDSIAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAIRKRNETSVIDQIGEDLLIWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSNLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLNTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQPPPCEGDNDEEEPPKLKVEHHDISVTGLQSPDRDLGLESPLMSSKPQSHSLGTSGKSEVDDLFVAERQFAKEQHADGTLKEVGVNYQITIPDPHLPVSEERWALDALRNLGLLKQLLVHQLGLTEKSTQEDWPHFPRCRTASLGVQADSGTQNPENIKACHPVEAQMPFRTGTGDISTCYSPRTSTESSAPRDSVVLAFQESQASDILVMDHMIMTPEMPPSEPEGGLDESGEHFFDAREAHSDDNPSEGDGAVKKEEKDVNLRISGTYLILDGYDPVQESSTDDEVASSFPLQPGAGIPSLDSGHQQQPSPQNAHSDGAVSPFNPELLVRQRWGAMEDSCFEIQSPSACADSQSQIMEYIRKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_006044891.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044891.2

ORF Insert Sequence:

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGTGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTACA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AAATATATGG TCTTGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTGTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCCCCTGGG TCGCCCCAGA TTCCACTTGC TGATTCTGAA 
GTAGAGCCAT CGGTCACTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGT 
GCACCTGGGA ATATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAAGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTT 
CAGCTATTGC AGGAAGACTA CAACCGAACA CCTGCTCAGA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC ATCCTCCAAG CCGTTAGCTG ACGGGCTAGC AGTGGGTGAA 
GGAGAAGCAG ATCCTGGTGA TGGGCTAGGC AGGCTTGACT ATGGCAGCGG AGACGCTTCT 
CGGCCCGGTA GTGACAGTGC CGATAGTCCC AAGAGTGGCT TGAAAGAGAG GATTTATCTA 
GAGGAAAACC CAGAAAAAAG TGAAGCAATT CAGGACACTG ACACTCAGTC GCTTGTTGGG 
AGTCCCTCTA CCCGTGTGGC ACCTCATATT ATTGGAGCAG AGGACGATGA CTTTGGTAGT 
GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTTCAGA GCATTGAGCT GCTAAAATCT 
CGCCCTGCTC ACTTGGCTGT CTTCTTACAC CATGTAGTTT CCCAGTTTGA CCCTGCAACA 
TTGCTCTGTT ACCTCTATTC AGACCTATAT AAACAGACCA ATTCCAAGGA GACCCGTCGT 
GTCTTCCTTG AGTTCCATCA GTTCTTCCTG GATCGATCTG CTCACCTGAA GGTTTCTGTT 
CCTGATGAAA TATCAGTAGA TCTAGAAAAA AGACGACCAG AGCTTATTCC TGAGGATCTA 
CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCACC CAGAAGTTCA GAAGCACTTG 
GAAGATTTTC GGCAGAAGCG CAGCATGGGG CTGACCCTGG CTGAAAGTGA ACTGACTAAA 
CTTGATGTAG AGCGAGACAA GGACCGGTTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA 
CAGATTGTTG CCAAAATTGA AGAAGTGTTG ATGACTGCTC AGGCTGTAGA AGAAGAAAAG 
AGTTCAACAA TGCAATATGT TATTCTCATG TACATGAAAC ATTTGGGAGT AAAAGTGAAA 
GAACCTCGAA ATTTGGAGCA CAAGCGAGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAA 
AGTATGAAGA AAGATAGAGA AGGCGAAGAA AAGGGGAAGC GAAGAGGGTT CACCAGCATC 
TTGGGACCCC CTAGGAGACC AAGCCGCCAC GACAATAGCG CAATTGGCAG AGCCATGGAA 
CTGCAGAAGC AGCGCCACCC TAAGCACTTG TCCACACCCT CGTCTGTGAG TCCTGAACCT 
CAGGATTCTG CGAAGCTGCG CCAGAGTGGA TCAGCAAGTG AAGGAGCAGA CAGTGGATAC 
CTGCCAGCCA ATTCCACGTC CTCCATGGTT TCAGGGGCCA CTCTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATATGGGATC AAAACAAGCT GGAGAAACGC CAGCATCTGG AGACTCCTTG 
GATGGCACTC CTCGTACTCC CAACACCATC TTTGATTTCC CACCTCCTCC ATTAGACCAG 
GTGCAGGAGG AGGAATGGGA AGTGGAGAGA GTGACTGAGC ATGGGACACC AAAGCCCTTT 
CGAAAGTTTG ACAGCATAGC TTTTGGAGAA AGTCAGAGTG AGGATGAACA GTTTGAAAAT 
GACTTAGAGA CGGACCCACC CAACTGGCAA CAGCTCGTCA GTCGAGAAGT GTTATTGGGA 
CTCAAGCCTT GTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACATTGAA GGTTCTAGAT CAAGTGTTTT ATCAGCGAGT GTCCAGAGAA 
GGAATTTTGT CACCTTCAGA GCTACGGAAA ATTTTTTCAA ACTTAGAAGA TATTCTTCAA 
CTTCACATTG GATTGAATGA GCAAATGAAG GCTATTCGGA AGAGGAATGA GACCTCTGTT 
ATTGATCAGA TTGGGGAAGA TTTGCTGATC TGGTTCAGTG GACCCGGGGA GGAAAAGTTG 
AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCCTTTG CCCTGGAAAT GATCAAGTCT 
CGCCAGAAAA AGGATTCTCG ATTCCAGACT TTTGTGCAAG ATGCTGAGAG TAATCCACTG 
TGTCGTCGTC TGCAGCTAAA GGATATCATT CCCACTCAAA TGCAGAGGCT TACTAAGTAC 
CCTCTTCTCT TGGACAATAT TGCCAAATAC ACAGAGTGGC CAACAGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTACG TGAACCAAGC TGTTAAGGAG 
GCAGAGAACA AACAGCGCTT GGAAGATTAT CAGCGTCGCC TTGATACCTC TAATCTGAAG 
TTGTCAGAAT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCACGAAG GCCCATTGGT TTGGAAGGTG AATAGAGATA AAACAATTGA TCTGTATACG 
TTGCTGCTGG AAGATATTCT TGTATTATTA CAAAAGCAGG ATGATAGACT GGTGTTAAGG 
TGTCACAGTA AGATTCTGGC ATCTACAGCT GACAGCAAAC ACACATTTAG CCCCGTCATC 
AAGCTGAATA CGGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATC 
TCCATGTCTG ATAATGGAGC CCAAATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTAAT ATGTCGGATG GCCGCGTCCG TGAAGGAGCA GTCCACAAAG 
CCGATCCCAC TGCCCCAGCC GCCACCGTGC GAAGGAGACA ACGATGAAGA AGAACCTCCA 
AAATTAAAAG TGGAACATCA TGACATTTCA GTCACTGGTC TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AGTCTCCTTT AATGTCATCA AAACCTCAGT CTCATTCGCT GGGTACCTCT 
GGGAAGTCGG AAGTAGATGA CCTCTTTGTG GCTGAGAGAC AGTTTGCAAA GGAGCAGCAT 
GCAGATGGGA CACTAAAGGA AGTTGGAGTG AATTATCAAA TCACAATCCC AGATCCACAC 
TTGCCTGTCT CGGAAGAACG GTGGGCGCTG GATGCACTAA GGAATTTGGG TTTGTTGAAG 
CAGCTGCTGG TACACCAGCT GGGCTTGACT GAGAAGAGCA CTCAGGAAGA CTGGCCACAT 
TTTCCAAGAT GCAGGACAGC CTCTCTGGGG GTGCAGGCAG ACAGTGGAAC CCAGAACCCT 
GAAAATATTA AGGCCTGTCA TCCTGTTGAA GCACAGATGC CCTTTAGAAC TGGAACTGGT 
GACATTTCAA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTCTGCTCC ACGGGATTCA 
GTAGTACTGG CATTCCAAGA GAGCCAGGCA AGTGACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTCC CTCAGAGCCA GAAGGGGGTC TTGATGAGAG TGGAGAGCAC 
TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT 
AAAAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA CCTATCTGAT CCTTGATGGC 
TATGACCCGG TGCAGGAGAG CTCCACAGAT GACGAGGTTG CTTCCTCGTT CCCCCTTCAG 
CCCGGGGCAG GCATCCCCTC TCTGGACTCT GGACACCAGC AGCAGCCTTC TCCCCAGAAT 
GCTCATTCTG ATGGGGCTGT TTCACCGTTC AACCCAGAAT TACTGGTCCG GCAGCGCTGG 
GGAGCTATGG AGGATTCCTG TTTTGAGATC CAGAGTCCCT CTGCTTGTGC TGATTCACAA 
AGCCAGATCA TGGAGTACAT TCGTAAAATA GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_025266750.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025122535.1 Bubalus bubalis Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu50703
Clone ID Related Accession (Same CDS sequence)	XM_025266750.1
Accession Version	XM_025266750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	rho guanine nucleotide exchange factor 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGTGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTACA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AAATATATGG TCTTGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTGTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCCCCTGGG TCGCCCCAGA TTCCACTTGC TGATTCTGAA 
GTAGAGCCAT CGGTCACTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGT 
GCACCTGGGA ATATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAAGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTT 
CAGCTATTGC AGGAAGACTA CAACCGAACA CCTGCTCAGA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC ATCCTCCAAG CCGTTAGCTG ACGGGCTAGC AGTGGGTGAA 
GGAGAAGCAG ATCCTGGTGA TGGGCTAGGC AGGCTTGACT ATGGCAGCGG AGACGCTTCT 
CGGCCCGGTA GTGACAGTGC CGATAGTGGC TTGAAAGAGA GGATTTATCT AGAGGAAAAC 
CCAGAAAAAA GTGAAGCAAT TCAGGACACT GACACTCAGT CGCTTGTTGG GAGTCCCTCT 
ACCCGTGTGG CACCTCATAT TATTGGAGCA GAGGACGATG ACTTTGGTAG TGAACATGAA 
CAGATCAATG GGCAGTGCAG CTGTTTTCAG AGCATTGAGC TGCTAAAATC TCGCCCTGCT 
CACTTGGCTG TCTTCTTACA CCATGTAGTT TCCCAGTTTG ACCCTGCAAC ATTGCTCTGT 
TACCTCTATT CAGACCTATA TAAACAGACC AATTCCAAGG AGACCCGTCG TGTCTTCCTT 
GAGTTCCATC AGTTCTTCCT GGATCGATCT GCTCACCTGA AGGTTTCTGT TCCTGATGAA 
ATATCAGTAG ATCTAGAAAA AAGACGACCA GAGCTTATTC CTGAGGATCT ACATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAC CCAGAAGTTC AGAAGCACTT GGAAGATTTT 
CGGCAGAAGC GCAGCATGGG GCTGACCCTG GCTGAAAGTG AACTGACTAA ACTTGATGTA 
GAGCGAGACA AGGACCGGTT AACTTTGGAG AAGGAACGGG CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTGTT GATGACTGCT CAGGCTGTAG AAGAAGAAAA GAGTTCAACA 
ATGCAATATG TTATTCTCAT GTACATGAAA CATTTGGGAG TAAAAGTGAA AGAACCTCGA 
AATTTGGAGC ACAAGCGAGG TCGGATTGGA TTCCTTCCAA AAATCAAGCA AAGTATGAAG 
AAAGATAGAG AAGGCGAAGA AAAGGGGAAG CGAAGAGGGT TCACCAGCAT CTTGGGACCC 
CCTAGGAGAC CAAGCCGCCA CGACAATAGC GCAATTGGCA GAGCCATGGA ACTGCAGAAG 
CAGCGCCACC CTAAGCACTT GTCCACACCC TCGTCTGTGA GTCCTGAACC TCAGGATTCT 
GCGAAGCTGC GCCAGAGTGG ATCAGCAAGT GAAGGAGCAG ACAGTGGATA CCTGCCAGCC 
AATTCCACGT CCTCCATGGT TTCAGGGGCC ACTCTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATATGGGAT CAAAACAAGC TGGAGAAACG CCAGCATCTG GAGACTCCTT GGATGGCACT 
CCTCGTACTC CCAACACCAT CTTTGATTTC CCACCTCCTC CATTAGACCA GGTGCAGGAG 
GAGGAATGGG AAGTGGAGAG AGTGACTGAG CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGCATAG CTTTTGGAGA AAGTCAGAGT GAGGATGAAC AGTTTGAAAA TGACTTAGAG 
ACGGACCCAC CCAACTGGCA ACAGCTCGTC AGTCGAGAAG TGTTATTGGG ACTCAAGCCT 
TGTGAGATCA AAAGACAGGA AGTAATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACATTGA AGGTTCTAGA TCAAGTGTTT TATCAGCGAG TGTCCAGAGA AGGAATTTTG 
TCACCTTCAG AGCTACGGAA AATTTTTTCA AACTTAGAAG ATATTCTTCA ACTTCACATT 
GGATTGAATG AGCAAATGAA GGCTATTCGG AAGAGGAATG AGACCTCTGT TATTGATCAG 
ATTGGGGAAG ATTTGCTGAT CTGGTTCAGT GGACCCGGGG AGGAAAAGTT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCCTTT GCCCTGGAAA TGATCAAGTC TCGCCAGAAA 
AAGGATTCTC GATTCCAGAC TTTTGTGCAA GATGCTGAGA GTAATCCACT GTGTCGTCGT 
CTGCAGCTAA AGGATATCAT TCCCACTCAA ATGCAGAGGC TTACTAAGTA CCCTCTTCTC 
TTGGACAATA TTGCCAAATA CACAGAGTGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAC GTGAACCAAG CTGTTAAGGA GGCAGAGAAC 
AAACAGCGCT TGGAAGATTA TCAGCGTCGC CTTGATACCT CTAATCTGAA GTTGTCAGAA 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCACGAA 
GGCCCATTGG TTTGGAAGGT GAATAGAGAT AAAACAATTG ATCTGTATAC GTTGCTGCTG 
GAAGATATTC TTGTATTATT ACAAAAGCAG GATGATAGAC TGGTGTTAAG GTGTCACAGT 
AAGATTCTGG CATCTACAGC TGACAGCAAA CACACATTTA GCCCCGTCAT CAAGCTGAAT 
ACGGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT CTCCATGTCT 
GATAATGGAG CCCAAATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TATGTCGGAT GGCCGCGTCC GTGAAGGAGC AGTCCACAAA GCCGATCCCA 
CTGCCCCAGC CGCCACCGTG CGAAGGAGAC AACGATGAAG AAGAACCTCC AAAATTAAAA 
GTGGAACATC ATGACATTTC AGTCACTGGT CTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAGTCTCCTT TAATGTCATC AAAACCTCAG TCTCATTCGC TGGGTACCTC TGGGAAGTCG 
GAAGTAGATG ACCTCTTTGT GGCTGAGAGA CAGTTTGCAA AGGAGCAGCA TGCAGATGGG 
ACACTAAAGG AAGTTGGAGT GAATTATCAA ATCACAATCC CAGATCCACA CTTGCCTGTC 
TCGGAAGAAC GGTGGGCGCT GGATGCACTA AGGAATTTGG GTTTGTTGAA GCAGCTGCTG 
GTACACCAGC TGGGCTTGAC TGAGAAGAGC ACTCAGGAAG ACTGGCCACA TTTTCCAAGA 
TGCAGGACAG CCTCTCTGGG GGTGCAGGCA GACAGTGGAA CCCAGAACCC TGAAAATATT 
AAGGCCTGTC ATCCTGTTGA AGCACAGATG CCCTTTAGAA CTGGAACTGG TGACATTTCA 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTCTGCTC CACGGGATTC AGTAGTACTG 
GCATTCCAAG AGAGCCAGGC AAGTGACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTC CCTCAGAGCC AGAAGGGGGT CTTGATGAGA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGATAATCCA TCAGAAGGTG ATGGAGCAGT TAAAAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA ACCTATCTGA TCCTTGATGG CTATGACCCG 
GTGCAGGAGA GCTCCACAGA TGACGAGGTT GCTTCCTCGT TCCCCCTTCA GCCCGGGGCA 
GGCATCCCCT CTCTGGACTC TGGACACCAG CAGCAGCCTT CTCCCCAGAA TGCTCATTCT 
GATGGGGCTG TTTCACCGTT CAACCCAGAA TTACTGGTCC GGCAGCGCTG GGGAGCTATG 
GAGGATTCCT GTTTTGAGAT CCAGAGTCCC TCTGCTTGTG CTGATTCACA AAGCCAGATC 
ATGGAGTACA TTCGTAAAAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025122535.1
CDS	145..4770
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERSTSHDFDPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVTGHMSPIMTSPHSPGAPGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTSSKPLADGLAVGEGEADPGDGLGRLDYGSGDASRPGSDSADSGLKERIYLEENPEKSEAIQDTDTQSLVGSPSTRVAPHIIGAEDDDFGSEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKQTNSKETRRVFLEFHQFFLDRSAHLKVSVPDEISVDLEKRRPELIPEDLHRHYIQTMQERVHPEVQKHLEDFRQKRSMGLTLAESELTKLDVERDKDRLTLEKERACAEQIVAKIEEVLMTAQAVEEEKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDREGEEKGKRRGFTSILGPPRRPSRHDNSAIGRAMELQKQRHPKHLSTPSSVSPEPQDSAKLRQSGSASEGADSGYLPANSTSSMVSGATLSQEGGKENDMGSKQAGETPASGDSLDGTPRTPNTIFDFPPPPLDQVQEEEWEVERVTEHGTPKPFRKFDSIAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAIRKRNETSVIDQIGEDLLIWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSNLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLNTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQPPPCEGDNDEEEPPKLKVEHHDISVTGLQSPDRDLGLESPLMSSKPQSHSLGTSGKSEVDDLFVAERQFAKEQHADGTLKEVGVNYQITIPDPHLPVSEERWALDALRNLGLLKQLLVHQLGLTEKSTQEDWPHFPRCRTASLGVQADSGTQNPENIKACHPVEAQMPFRTGTGDISTCYSPRTSTESSAPRDSVVLAFQESQASDILVMDHMIMTPEMPPSEPEGGLDESGEHFFDAREAHSDDNPSEGDGAVKKEEKDVNLRISGTYLILDGYDPVQESSTDDEVASSFPLQPGAGIPSLDSGHQQQPSPQNAHSDGAVSPFNPELLVRQRWGAMEDSCFEIQSPSACADSQSQIMEYIRKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_025266750.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025266750.1

ORF Insert Sequence:

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGTGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTACA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AAATATATGG TCTTGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTGTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCCCCTGGG TCGCCCCAGA TTCCACTTGC TGATTCTGAA 
GTAGAGCCAT CGGTCACTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGT 
GCACCTGGGA ATATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAAGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTT 
CAGCTATTGC AGGAAGACTA CAACCGAACA CCTGCTCAGA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC ATCCTCCAAG CCGTTAGCTG ACGGGCTAGC AGTGGGTGAA 
GGAGAAGCAG ATCCTGGTGA TGGGCTAGGC AGGCTTGACT ATGGCAGCGG AGACGCTTCT 
CGGCCCGGTA GTGACAGTGC CGATAGTGGC TTGAAAGAGA GGATTTATCT AGAGGAAAAC 
CCAGAAAAAA GTGAAGCAAT TCAGGACACT GACACTCAGT CGCTTGTTGG GAGTCCCTCT 
ACCCGTGTGG CACCTCATAT TATTGGAGCA GAGGACGATG ACTTTGGTAG TGAACATGAA 
CAGATCAATG GGCAGTGCAG CTGTTTTCAG AGCATTGAGC TGCTAAAATC TCGCCCTGCT 
CACTTGGCTG TCTTCTTACA CCATGTAGTT TCCCAGTTTG ACCCTGCAAC ATTGCTCTGT 
TACCTCTATT CAGACCTATA TAAACAGACC AATTCCAAGG AGACCCGTCG TGTCTTCCTT 
GAGTTCCATC AGTTCTTCCT GGATCGATCT GCTCACCTGA AGGTTTCTGT TCCTGATGAA 
ATATCAGTAG ATCTAGAAAA AAGACGACCA GAGCTTATTC CTGAGGATCT ACATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAC CCAGAAGTTC AGAAGCACTT GGAAGATTTT 
CGGCAGAAGC GCAGCATGGG GCTGACCCTG GCTGAAAGTG AACTGACTAA ACTTGATGTA 
GAGCGAGACA AGGACCGGTT AACTTTGGAG AAGGAACGGG CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTGTT GATGACTGCT CAGGCTGTAG AAGAAGAAAA GAGTTCAACA 
ATGCAATATG TTATTCTCAT GTACATGAAA CATTTGGGAG TAAAAGTGAA AGAACCTCGA 
AATTTGGAGC ACAAGCGAGG TCGGATTGGA TTCCTTCCAA AAATCAAGCA AAGTATGAAG 
AAAGATAGAG AAGGCGAAGA AAAGGGGAAG CGAAGAGGGT TCACCAGCAT CTTGGGACCC 
CCTAGGAGAC CAAGCCGCCA CGACAATAGC GCAATTGGCA GAGCCATGGA ACTGCAGAAG 
CAGCGCCACC CTAAGCACTT GTCCACACCC TCGTCTGTGA GTCCTGAACC TCAGGATTCT 
GCGAAGCTGC GCCAGAGTGG ATCAGCAAGT GAAGGAGCAG ACAGTGGATA CCTGCCAGCC 
AATTCCACGT CCTCCATGGT TTCAGGGGCC ACTCTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATATGGGAT CAAAACAAGC TGGAGAAACG CCAGCATCTG GAGACTCCTT GGATGGCACT 
CCTCGTACTC CCAACACCAT CTTTGATTTC CCACCTCCTC CATTAGACCA GGTGCAGGAG 
GAGGAATGGG AAGTGGAGAG AGTGACTGAG CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGCATAG CTTTTGGAGA AAGTCAGAGT GAGGATGAAC AGTTTGAAAA TGACTTAGAG 
ACGGACCCAC CCAACTGGCA ACAGCTCGTC AGTCGAGAAG TGTTATTGGG ACTCAAGCCT 
TGTGAGATCA AAAGACAGGA AGTAATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACATTGA AGGTTCTAGA TCAAGTGTTT TATCAGCGAG TGTCCAGAGA AGGAATTTTG 
TCACCTTCAG AGCTACGGAA AATTTTTTCA AACTTAGAAG ATATTCTTCA ACTTCACATT 
GGATTGAATG AGCAAATGAA GGCTATTCGG AAGAGGAATG AGACCTCTGT TATTGATCAG 
ATTGGGGAAG ATTTGCTGAT CTGGTTCAGT GGACCCGGGG AGGAAAAGTT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCCTTT GCCCTGGAAA TGATCAAGTC TCGCCAGAAA 
AAGGATTCTC GATTCCAGAC TTTTGTGCAA GATGCTGAGA GTAATCCACT GTGTCGTCGT 
CTGCAGCTAA AGGATATCAT TCCCACTCAA ATGCAGAGGC TTACTAAGTA CCCTCTTCTC 
TTGGACAATA TTGCCAAATA CACAGAGTGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAC GTGAACCAAG CTGTTAAGGA GGCAGAGAAC 
AAACAGCGCT TGGAAGATTA TCAGCGTCGC CTTGATACCT CTAATCTGAA GTTGTCAGAA 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCACGAA 
GGCCCATTGG TTTGGAAGGT GAATAGAGAT AAAACAATTG ATCTGTATAC GTTGCTGCTG 
GAAGATATTC TTGTATTATT ACAAAAGCAG GATGATAGAC TGGTGTTAAG GTGTCACAGT 
AAGATTCTGG CATCTACAGC TGACAGCAAA CACACATTTA GCCCCGTCAT CAAGCTGAAT 
ACGGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT CTCCATGTCT 
GATAATGGAG CCCAAATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TATGTCGGAT GGCCGCGTCC GTGAAGGAGC AGTCCACAAA GCCGATCCCA 
CTGCCCCAGC CGCCACCGTG CGAAGGAGAC AACGATGAAG AAGAACCTCC AAAATTAAAA 
GTGGAACATC ATGACATTTC AGTCACTGGT CTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAGTCTCCTT TAATGTCATC AAAACCTCAG TCTCATTCGC TGGGTACCTC TGGGAAGTCG 
GAAGTAGATG ACCTCTTTGT GGCTGAGAGA CAGTTTGCAA AGGAGCAGCA TGCAGATGGG 
ACACTAAAGG AAGTTGGAGT GAATTATCAA ATCACAATCC CAGATCCACA CTTGCCTGTC 
TCGGAAGAAC GGTGGGCGCT GGATGCACTA AGGAATTTGG GTTTGTTGAA GCAGCTGCTG 
GTACACCAGC TGGGCTTGAC TGAGAAGAGC ACTCAGGAAG ACTGGCCACA TTTTCCAAGA 
TGCAGGACAG CCTCTCTGGG GGTGCAGGCA GACAGTGGAA CCCAGAACCC TGAAAATATT 
AAGGCCTGTC ATCCTGTTGA AGCACAGATG CCCTTTAGAA CTGGAACTGG TGACATTTCA 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTCTGCTC CACGGGATTC AGTAGTACTG 
GCATTCCAAG AGAGCCAGGC AAGTGACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTC CCTCAGAGCC AGAAGGGGGT CTTGATGAGA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGATAATCCA TCAGAAGGTG ATGGAGCAGT TAAAAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA ACCTATCTGA TCCTTGATGG CTATGACCCG 
GTGCAGGAGA GCTCCACAGA TGACGAGGTT GCTTCCTCGT TCCCCCTTCA GCCCGGGGCA 
GGCATCCCCT CTCTGGACTC TGGACACCAG CAGCAGCCTT CTCCCCAGAA TGCTCATTCT 
GATGGGGCTG TTTCACCGTT CAACCCAGAA TTACTGGTCC GGCAGCGCTG GGGAGCTATG 
GAGGATTCCT GTTTTGAGAT CCAGAGTCCC TCTGCTTGTG CTGATTCACA AAGCCAGATC 
ATGGAGTACA TTCGTAAAAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_025266751.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025122536.1 Bubalus bubalis Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu50704
Clone ID Related Accession (Same CDS sequence)	XM_025266751.1
Accession Version	XM_025266751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	rho guanine nucleotide exchange factor 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGTGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTACA 
TCACATGATT TTGACCCCAC AGGTCTTGTT CAGCGTTGTG TGATCATCCA GAAGGATGAC 
AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTGT TCGTACAGTC TGTCAAAGAA 
GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA 
ACTCTGGTGA CTCATTCAAA CCATTTGGAA GTGGTGAAGC TAATCAAATC TGGTTCCTAT 
GTAGCTCTCA CTGTTCAGGG ACGCCCCCCT GGGTCGCCCC AGATTCCACT TGCTGATTCT 
GAAGTAGAGC CATCGGTCAC TGGACATATG TCTCCCATCA TGACATCCCC TCATTCACCT 
GGTGCACCTG GGAATATGGA GAGAATTACT AGTCCTGTGC TCATGGGGGA AGAAAACAAT 
GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAAATGT TACAGAAAGA ACAGGAACGG 
CTTCAGCTAT TGCAGGAAGA CTACAACCGA ACACCTGCTC AGAGATTGCT AAAAGAGATC 
CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT 
GCTCAGGATG GAGCTGTAGT TACATCCTCC AAGCCGTTAG CTGACGGGCT AGCAGTGGGT 
GAAGGAGAAG CAGATCCTGG TGATGGGCTA GGCAGGCTTG ACTATGGCAG CGGAGACGCT 
TCTCGGCCCG GTAGTGACAG TGCCGATAGT CCCAAGAGTG GCTTGAAAGA GAGGATTTAT 
CTAGAGGAAA ACCCAGAAAA AAGTGAAGCA ATTCAGGACA CTGACACTCA GTCGCTTGTT 
GGGAGTCCCT CTACCCGTGT GGCACCTCAT ATTATTGGAG CAGAGGACGA TGACTTTGGT 
AGTGAACATG AACAGATCAA TGGGCAGTGC AGCTGTTTTC AGAGCATTGA GCTGCTAAAA 
TCTCGCCCTG CTCACTTGGC TGTCTTCTTA CACCATGTAG TTTCCCAGTT TGACCCTGCA 
ACATTGCTCT GTTACCTCTA TTCAGACCTA TATAAACAGA CCAATTCCAA GGAGACCCGT 
CGTGTCTTCC TTGAGTTCCA TCAGTTCTTC CTGGATCGAT CTGCTCACCT GAAGGTTTCT 
GTTCCTGATG AAATATCAGT AGATCTAGAA AAAAGACGAC CAGAGCTTAT TCCTGAGGAT 
CTACATCGCC ACTATATCCA AACTATGCAA GAAAGAGTCC ACCCAGAAGT TCAGAAGCAC 
TTGGAAGATT TTCGGCAGAA GCGCAGCATG GGGCTGACCC TGGCTGAAAG TGAACTGACT 
AAACTTGATG TAGAGCGAGA CAAGGACCGG TTAACTTTGG AGAAGGAACG GGCATGTGCA 
GAACAGATTG TTGCCAAAAT TGAAGAAGTG TTGATGACTG CTCAGGCTGT AGAAGAAGAA 
AAGAGTTCAA CAATGCAATA TGTTATTCTC ATGTACATGA AACATTTGGG AGTAAAAGTG 
AAAGAACCTC GAAATTTGGA GCACAAGCGA GGTCGGATTG GATTCCTTCC AAAAATCAAG 
CAAAGTATGA AGAAAGATAG AGAAGGCGAA GAAAAGGGGA AGCGAAGAGG GTTCACCAGC 
ATCTTGGGAC CCCCTAGGAG ACCAAGCCGC CACGACAATA GCGCAATTGG CAGAGCCATG 
GAACTGCAGA AGCAGCGCCA CCCTAAGCAC TTGTCCACAC CCTCGTCTGT GAGTCCTGAA 
CCTCAGGATT CTGCGAAGCT GCGCCAGAGT GGATCAGCAA GTGAAGGAGC AGACAGTGGA 
TACCTGCCAG CCAATTCCAC GTCCTCCATG GTTTCAGGGG CCACTCTTTC CCAGGAAGGA 
GGGAAAGAGA ATGATATGGG ATCAAAACAA GCTGGAGAAA CGCCAGCATC TGGAGACTCC 
TTGGATGGCA CTCCTCGTAC TCCCAACACC ATCTTTGATT TCCCACCTCC TCCATTAGAC 
CAGGTGCAGG AGGAGGAATG GGAAGTGGAG AGAGTGACTG AGCATGGGAC ACCAAAGCCC 
TTTCGAAAGT TTGACAGCAT AGCTTTTGGA GAAAGTCAGA GTGAGGATGA ACAGTTTGAA 
AATGACTTAG AGACGGACCC ACCCAACTGG CAACAGCTCG TCAGTCGAGA AGTGTTATTG 
GGACTCAAGC CTTGTGAGAT CAAAAGACAG GAAGTAATTA ATGAATTGTT CTACACTGAA 
AGAGCTCATG TTCGAACATT GAAGGTTCTA GATCAAGTGT TTTATCAGCG AGTGTCCAGA 
GAAGGAATTT TGTCACCTTC AGAGCTACGG AAAATTTTTT CAAACTTAGA AGATATTCTT 
CAACTTCACA TTGGATTGAA TGAGCAAATG AAGGCTATTC GGAAGAGGAA TGAGACCTCT 
GTTATTGATC AGATTGGGGA AGATTTGCTG ATCTGGTTCA GTGGACCCGG GGAGGAAAAG 
TTGAAACATG CTGCTGCTAC CTTTTGCAGT AACCAACCCT TTGCCCTGGA AATGATCAAG 
TCTCGCCAGA AAAAGGATTC TCGATTCCAG ACTTTTGTGC AAGATGCTGA GAGTAATCCA 
CTGTGTCGTC GTCTGCAGCT AAAGGATATC ATTCCCACTC AAATGCAGAG GCTTACTAAG 
TACCCTCTTC TCTTGGACAA TATTGCCAAA TACACAGAGT GGCCAACAGA AAGGGAGAAG 
GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAATT ACGTGAACCA AGCTGTTAAG 
GAGGCAGAGA ACAAACAGCG CTTGGAAGAT TATCAGCGTC GCCTTGATAC CTCTAATCTG 
AAGTTGTCAG AATACCCAAA TGTTGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG 
ATGATTCACG AAGGCCCATT GGTTTGGAAG GTGAATAGAG ATAAAACAAT TGATCTGTAT 
ACGTTGCTGC TGGAAGATAT TCTTGTATTA TTACAAAAGC AGGATGATAG ACTGGTGTTA 
AGGTGTCACA GTAAGATTCT GGCATCTACA GCTGACAGCA AACACACATT TAGCCCCGTC 
ATCAAGCTGA ATACGGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTCGTC 
ATCTCCATGT CTGATAATGG AGCCCAAATT TATGAACTGG TGGCACAGAC AGTTTCTGAA 
AAGACTGTCT GGCAGGACCT AATATGTCGG ATGGCCGCGT CCGTGAAGGA GCAGTCCACA 
AAGCCGATCC CACTGCCCCA GCCGCCACCG TGCGAAGGAG ACAACGATGA AGAAGAACCT 
CCAAAATTAA AAGTGGAACA TCATGACATT TCAGTCACTG GTCTGCAGAG TCCAGACAGA 
GATTTGGGAT TAGAGTCTCC TTTAATGTCA TCAAAACCTC AGTCTCATTC GCTGGGTACC 
TCTGGGAAGT CGGAAGTAGA TGACCTCTTT GTGGCTGAGA GACAGTTTGC AAAGGAGCAG 
CATGCAGATG GGACACTAAA GGAAGTTGGA GTGAATTATC AAATCACAAT CCCAGATCCA 
CACTTGCCTG TCTCGGAAGA ACGGTGGGCG CTGGATGCAC TAAGGAATTT GGGTTTGTTG 
AAGCAGCTGC TGGTACACCA GCTGGGCTTG ACTGAGAAGA GCACTCAGGA AGACTGGCCA 
CATTTTCCAA GATGCAGGAC AGCCTCTCTG GGGGTGCAGG CAGACAGTGG AACCCAGAAC 
CCTGAAAATA TTAAGGCCTG TCATCCTGTT GAAGCACAGA TGCCCTTTAG AACTGGAACT 
GGTGACATTT CAACTTGTTA CAGTCCACGG ACTTCAACTG AATCTTCTGC TCCACGGGAT 
TCAGTAGTAC TGGCATTCCA AGAGAGCCAG GCAAGTGACA TTTTAGTAAT GGACCACATG 
ATTATGACCC CAGAGATGCC TCCCTCAGAG CCAGAAGGGG GTCTTGATGA GAGTGGAGAG 
CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGATAATC CATCAGAAGG TGATGGAGCA 
GTTAAAAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAACCTATCT GATCCTTGAT 
GGCTATGACC CGGTGCAGGA GAGCTCCACA GATGACGAGG TTGCTTCCTC GTTCCCCCTT 
CAGCCCGGGG CAGGCATCCC CTCTCTGGAC TCTGGACACC AGCAGCAGCC TTCTCCCCAG 
AATGCTCATT CTGATGGGGC TGTTTCACCG TTCAACCCAG AATTACTGGT CCGGCAGCGC 
TGGGGAGCTA TGGAGGATTC CTGTTTTGAG ATCCAGAGTC CCTCTGCTTG TGCTGATTCA 
CAAAGCCAGA TCATGGAGTA CATTCGTAAA ATAGAGGCTG ACCTTGAACA CTTAAAGAAG 
GTGGAGGAAA GTTACACCAT TCTTTGCCAA AGGCTGGCTG GATCAGCCCT CACAGACAAG 
CACTCAGATA AAAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025122536.1
CDS	145..4722
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERSTSHDFDPTGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVTGHMSPIMTSPHSPGAPGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTSSKPLADGLAVGEGEADPGDGLGRLDYGSGDASRPGSDSADSPKSGLKERIYLEENPEKSEAIQDTDTQSLVGSPSTRVAPHIIGAEDDDFGSEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKQTNSKETRRVFLEFHQFFLDRSAHLKVSVPDEISVDLEKRRPELIPEDLHRHYIQTMQERVHPEVQKHLEDFRQKRSMGLTLAESELTKLDVERDKDRLTLEKERACAEQIVAKIEEVLMTAQAVEEEKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDREGEEKGKRRGFTSILGPPRRPSRHDNSAIGRAMELQKQRHPKHLSTPSSVSPEPQDSAKLRQSGSASEGADSGYLPANSTSSMVSGATLSQEGGKENDMGSKQAGETPASGDSLDGTPRTPNTIFDFPPPPLDQVQEEEWEVERVTEHGTPKPFRKFDSIAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAIRKRNETSVIDQIGEDLLIWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSNLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLNTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQPPPCEGDNDEEEPPKLKVEHHDISVTGLQSPDRDLGLESPLMSSKPQSHSLGTSGKSEVDDLFVAERQFAKEQHADGTLKEVGVNYQITIPDPHLPVSEERWALDALRNLGLLKQLLVHQLGLTEKSTQEDWPHFPRCRTASLGVQADSGTQNPENIKACHPVEAQMPFRTGTGDISTCYSPRTSTESSAPRDSVVLAFQESQASDILVMDHMIMTPEMPPSEPEGGLDESGEHFFDAREAHSDDNPSEGDGAVKKEEKDVNLRISGTYLILDGYDPVQESSTDDEVASSFPLQPGAGIPSLDSGHQQQPSPQNAHSDGAVSPFNPELLVRQRWGAMEDSCFEIQSPSACADSQSQIMEYIRKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_025266751.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025266751.1

ORF Insert Sequence:

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGTGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTACA 
TCACATGATT TTGACCCCAC AGGTCTTGTT CAGCGTTGTG TGATCATCCA GAAGGATGAC 
AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTGT TCGTACAGTC TGTCAAAGAA 
GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA 
ACTCTGGTGA CTCATTCAAA CCATTTGGAA GTGGTGAAGC TAATCAAATC TGGTTCCTAT 
GTAGCTCTCA CTGTTCAGGG ACGCCCCCCT GGGTCGCCCC AGATTCCACT TGCTGATTCT 
GAAGTAGAGC CATCGGTCAC TGGACATATG TCTCCCATCA TGACATCCCC TCATTCACCT 
GGTGCACCTG GGAATATGGA GAGAATTACT AGTCCTGTGC TCATGGGGGA AGAAAACAAT 
GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAAATGT TACAGAAAGA ACAGGAACGG 
CTTCAGCTAT TGCAGGAAGA CTACAACCGA ACACCTGCTC AGAGATTGCT AAAAGAGATC 
CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT 
GCTCAGGATG GAGCTGTAGT TACATCCTCC AAGCCGTTAG CTGACGGGCT AGCAGTGGGT 
GAAGGAGAAG CAGATCCTGG TGATGGGCTA GGCAGGCTTG ACTATGGCAG CGGAGACGCT 
TCTCGGCCCG GTAGTGACAG TGCCGATAGT CCCAAGAGTG GCTTGAAAGA GAGGATTTAT 
CTAGAGGAAA ACCCAGAAAA AAGTGAAGCA ATTCAGGACA CTGACACTCA GTCGCTTGTT 
GGGAGTCCCT CTACCCGTGT GGCACCTCAT ATTATTGGAG CAGAGGACGA TGACTTTGGT 
AGTGAACATG AACAGATCAA TGGGCAGTGC AGCTGTTTTC AGAGCATTGA GCTGCTAAAA 
TCTCGCCCTG CTCACTTGGC TGTCTTCTTA CACCATGTAG TTTCCCAGTT TGACCCTGCA 
ACATTGCTCT GTTACCTCTA TTCAGACCTA TATAAACAGA CCAATTCCAA GGAGACCCGT 
CGTGTCTTCC TTGAGTTCCA TCAGTTCTTC CTGGATCGAT CTGCTCACCT GAAGGTTTCT 
GTTCCTGATG AAATATCAGT AGATCTAGAA AAAAGACGAC CAGAGCTTAT TCCTGAGGAT 
CTACATCGCC ACTATATCCA AACTATGCAA GAAAGAGTCC ACCCAGAAGT TCAGAAGCAC 
TTGGAAGATT TTCGGCAGAA GCGCAGCATG GGGCTGACCC TGGCTGAAAG TGAACTGACT 
AAACTTGATG TAGAGCGAGA CAAGGACCGG TTAACTTTGG AGAAGGAACG GGCATGTGCA 
GAACAGATTG TTGCCAAAAT TGAAGAAGTG TTGATGACTG CTCAGGCTGT AGAAGAAGAA 
AAGAGTTCAA CAATGCAATA TGTTATTCTC ATGTACATGA AACATTTGGG AGTAAAAGTG 
AAAGAACCTC GAAATTTGGA GCACAAGCGA GGTCGGATTG GATTCCTTCC AAAAATCAAG 
CAAAGTATGA AGAAAGATAG AGAAGGCGAA GAAAAGGGGA AGCGAAGAGG GTTCACCAGC 
ATCTTGGGAC CCCCTAGGAG ACCAAGCCGC CACGACAATA GCGCAATTGG CAGAGCCATG 
GAACTGCAGA AGCAGCGCCA CCCTAAGCAC TTGTCCACAC CCTCGTCTGT GAGTCCTGAA 
CCTCAGGATT CTGCGAAGCT GCGCCAGAGT GGATCAGCAA GTGAAGGAGC AGACAGTGGA 
TACCTGCCAG CCAATTCCAC GTCCTCCATG GTTTCAGGGG CCACTCTTTC CCAGGAAGGA 
GGGAAAGAGA ATGATATGGG ATCAAAACAA GCTGGAGAAA CGCCAGCATC TGGAGACTCC 
TTGGATGGCA CTCCTCGTAC TCCCAACACC ATCTTTGATT TCCCACCTCC TCCATTAGAC 
CAGGTGCAGG AGGAGGAATG GGAAGTGGAG AGAGTGACTG AGCATGGGAC ACCAAAGCCC 
TTTCGAAAGT TTGACAGCAT AGCTTTTGGA GAAAGTCAGA GTGAGGATGA ACAGTTTGAA 
AATGACTTAG AGACGGACCC ACCCAACTGG CAACAGCTCG TCAGTCGAGA AGTGTTATTG 
GGACTCAAGC CTTGTGAGAT CAAAAGACAG GAAGTAATTA ATGAATTGTT CTACACTGAA 
AGAGCTCATG TTCGAACATT GAAGGTTCTA GATCAAGTGT TTTATCAGCG AGTGTCCAGA 
GAAGGAATTT TGTCACCTTC AGAGCTACGG AAAATTTTTT CAAACTTAGA AGATATTCTT 
CAACTTCACA TTGGATTGAA TGAGCAAATG AAGGCTATTC GGAAGAGGAA TGAGACCTCT 
GTTATTGATC AGATTGGGGA AGATTTGCTG ATCTGGTTCA GTGGACCCGG GGAGGAAAAG 
TTGAAACATG CTGCTGCTAC CTTTTGCAGT AACCAACCCT TTGCCCTGGA AATGATCAAG 
TCTCGCCAGA AAAAGGATTC TCGATTCCAG ACTTTTGTGC AAGATGCTGA GAGTAATCCA 
CTGTGTCGTC GTCTGCAGCT AAAGGATATC ATTCCCACTC AAATGCAGAG GCTTACTAAG 
TACCCTCTTC TCTTGGACAA TATTGCCAAA TACACAGAGT GGCCAACAGA AAGGGAGAAG 
GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAATT ACGTGAACCA AGCTGTTAAG 
GAGGCAGAGA ACAAACAGCG CTTGGAAGAT TATCAGCGTC GCCTTGATAC CTCTAATCTG 
AAGTTGTCAG AATACCCAAA TGTTGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG 
ATGATTCACG AAGGCCCATT GGTTTGGAAG GTGAATAGAG ATAAAACAAT TGATCTGTAT 
ACGTTGCTGC TGGAAGATAT TCTTGTATTA TTACAAAAGC AGGATGATAG ACTGGTGTTA 
AGGTGTCACA GTAAGATTCT GGCATCTACA GCTGACAGCA AACACACATT TAGCCCCGTC 
ATCAAGCTGA ATACGGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTCGTC 
ATCTCCATGT CTGATAATGG AGCCCAAATT TATGAACTGG TGGCACAGAC AGTTTCTGAA 
AAGACTGTCT GGCAGGACCT AATATGTCGG ATGGCCGCGT CCGTGAAGGA GCAGTCCACA 
AAGCCGATCC CACTGCCCCA GCCGCCACCG TGCGAAGGAG ACAACGATGA AGAAGAACCT 
CCAAAATTAA AAGTGGAACA TCATGACATT TCAGTCACTG GTCTGCAGAG TCCAGACAGA 
GATTTGGGAT TAGAGTCTCC TTTAATGTCA TCAAAACCTC AGTCTCATTC GCTGGGTACC 
TCTGGGAAGT CGGAAGTAGA TGACCTCTTT GTGGCTGAGA GACAGTTTGC AAAGGAGCAG 
CATGCAGATG GGACACTAAA GGAAGTTGGA GTGAATTATC AAATCACAAT CCCAGATCCA 
CACTTGCCTG TCTCGGAAGA ACGGTGGGCG CTGGATGCAC TAAGGAATTT GGGTTTGTTG 
AAGCAGCTGC TGGTACACCA GCTGGGCTTG ACTGAGAAGA GCACTCAGGA AGACTGGCCA 
CATTTTCCAA GATGCAGGAC AGCCTCTCTG GGGGTGCAGG CAGACAGTGG AACCCAGAAC 
CCTGAAAATA TTAAGGCCTG TCATCCTGTT GAAGCACAGA TGCCCTTTAG AACTGGAACT 
GGTGACATTT CAACTTGTTA CAGTCCACGG ACTTCAACTG AATCTTCTGC TCCACGGGAT 
TCAGTAGTAC TGGCATTCCA AGAGAGCCAG GCAAGTGACA TTTTAGTAAT GGACCACATG 
ATTATGACCC CAGAGATGCC TCCCTCAGAG CCAGAAGGGG GTCTTGATGA GAGTGGAGAG 
CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGATAATC CATCAGAAGG TGATGGAGCA 
GTTAAAAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAACCTATCT GATCCTTGAT 
GGCTATGACC CGGTGCAGGA GAGCTCCACA GATGACGAGG TTGCTTCCTC GTTCCCCCTT 
CAGCCCGGGG CAGGCATCCC CTCTCTGGAC TCTGGACACC AGCAGCAGCC TTCTCCCCAG 
AATGCTCATT CTGATGGGGC TGTTTCACCG TTCAACCCAG AATTACTGGT CCGGCAGCGC 
TGGGGAGCTA TGGAGGATTC CTGTTTTGAG ATCCAGAGTC CCTCTGCTTG TGCTGATTCA 
CAAAGCCAGA TCATGGAGTA CATTCGTAAA ATAGAGGCTG ACCTTGAACA CTTAAAGAAG 
GTGGAGGAAA GTTACACCAT TCTTTGCCAA AGGCTGGCTG GATCAGCCCT CACAGACAAG 
CACTCAGATA AAAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

ARHGEF12 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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