NCAM1 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following NCAM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCAM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu26733 XM_006072489.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu26734 XM_006072490.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu26735 XM_006072491.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu26736 XM_006072492.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu26737 XM_006072493.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu26738 XM_006072494.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBu26739 XM_006072495.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBu26740 XM_006072496.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBu50839 XM_006072489.2
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu50840 XM_006072490.2
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu50841 XM_006072491.2
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu50842 XM_006072492.2
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu50843 XM_006072493.2
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu50844 XM_006072494.2
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBu50845 XM_006072495.2
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBu50846 XM_025266888.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBu50847 XM_025266889.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBu50848 XM_006072496.2
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBu50850 XM_025266891.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBu50851 XM_025266892.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBu26728 XM_006072484.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBu26729 XM_006072485.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBu26730 XM_006072486.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBu26731 XM_006072487.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBu26732 XM_006072488.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBu50828 XM_006072484.2
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBu50829 XM_025266882.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBu50830 XM_025266883.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBu50831 XM_006072485.2
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBu50832 XM_025266884.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBu50833 XM_006072486.2
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBu50834 XM_025266885.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBu50835 XM_025266886.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBu50836 XM_006072487.2
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBu50837 XM_006072488.2
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBu50838 XM_025266887.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBu50849 XM_025266890.1
Latest version!
Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu26733
    Clone ID Related Accession (Same CDS sequence) XM_006072489.1
    Accession Version XM_006072489.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2673bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785492.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCTTC CTGCTCTTAC AACCACGGAA
    GCAACTAAAG GGGAACCCAG CGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGAAGCT CTCCTCCGAG TGGAAACCAG AGATCAGGCT CCCGTCTGGC
    AGTGACCACG TCATGCTCAA GTCCCTGGAC TGGAATGCCG AGTACGAGGT CTATGTGGTG
    GCCGAGAACC AGCAGGGCAA GTCCAAGGCG GCACACTTTG TGTTCAGGAC CTCGGCCCAG
    CCCACAGCCA TCCCAGCCAA CGGCAGCCCC ACCTCGGGCC TGAGCACTGG CGCCATCGTG
    GGCATCCTCG TCGTGACCTT CGTCCTGCTC CTGGTGGCCG TGGACGTCAC CTGCTACTTC
    CTGAACAAGT GTGGCCTGCT CATGTGCATT GCCGTCAACC TGTGTGGCAA AGCCGGGCCC
    GGAGCCAAGG GCAAGGACAT GGAGGAGGGC AAAGCTGCCT TCTCGAAAGA CGAGTCCAAG
    GAGCCTATCG TGGAGGTGCG AACGGAGGAG GAGCGGACCC CGAATCACGA CGGAGGGAAA
    CACACAGAGC CGAACGAGAC CACACCGCTG ACGGAGCCCG AGAAGGGTCC CGTAGAAGCA
    AAGCCGGAGA CAGAAACAAA GCCAGCGCCA GCCGAAGTCC AGACGGTCCC CAACGATGCC
    ACACAAATAA AGGAGAATGA GAGCAAAGCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072551.1
    CDS281..2953
    Translation

    Target ORF information:

    RefSeq Version XM_006072489.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072489.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCTTC CTGCTCTTAC AACCACGGAA
    GCAACTAAAG GGGAACCCAG CGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGAAGCT CTCCTCCGAG TGGAAACCAG AGATCAGGCT CCCGTCTGGC
    AGTGACCACG TCATGCTCAA GTCCCTGGAC TGGAATGCCG AGTACGAGGT CTATGTGGTG
    GCCGAGAACC AGCAGGGCAA GTCCAAGGCG GCACACTTTG TGTTCAGGAC CTCGGCCCAG
    CCCACAGCCA TCCCAGCCAA CGGCAGCCCC ACCTCGGGCC TGAGCACTGG CGCCATCGTG
    GGCATCCTCG TCGTGACCTT CGTCCTGCTC CTGGTGGCCG TGGACGTCAC CTGCTACTTC
    CTGAACAAGT GTGGCCTGCT CATGTGCATT GCCGTCAACC TGTGTGGCAA AGCCGGGCCC
    GGAGCCAAGG GCAAGGACAT GGAGGAGGGC AAAGCTGCCT TCTCGAAAGA CGAGTCCAAG
    GAGCCTATCG TGGAGGTGCG AACGGAGGAG GAGCGGACCC CGAATCACGA CGGAGGGAAA
    CACACAGAGC CGAACGAGAC CACACCGCTG ACGGAGCCCG AGAAGGGTCC CGTAGAAGCA
    AAGCCGGAGA CAGAAACAAA GCCAGCGCCA GCCGAAGTCC AGACGGTCCC CAACGATGCC
    ACACAAATAA AGGAGAATGA GAGCAAAGCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu26734
    Clone ID Related Accession (Same CDS sequence) XM_006072490.1
    Accession Version XM_006072490.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785492.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA CCCAGCGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAGG CGGCACACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCC AACGGCAGCC CCACCTCGGG CCTGAGCACT
    GGCGCCATCG TGGGCATCCT CGTCGTGACC TTCGTCCTGC TCCTGGTGGC CGTGGACGTC
    ACCTGCTACT TCCTGAACAA GTGTGGCCTG CTCATGTGCA TTGCCGTCAA CCTGTGTGGC
    AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC ATGGAGGAGG GCAAAGCTGC CTTCTCGAAA
    GACGAGTCCA AGGAGCCTAT CGTGGAGGTG CGAACGGAGG AGGAGCGGAC CCCGAATCAC
    GACGGAGGGA AACACACAGA GCCGAACGAG ACCACACCGC TGACGGAGCC CGAGAAGGGT
    CCCGTAGAAG CAAAGCCGGA GACAGAAACA AAGCCAGCGC CAGCCGAAGT CCAGACGGTC
    CCCAACGATG CCACACAAAT AAAGGAGAAT GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072552.1
    CDS280..2844
    Translation

    Target ORF information:

    RefSeq Version XM_006072490.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072490.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA CCCAGCGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAGG CGGCACACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCC AACGGCAGCC CCACCTCGGG CCTGAGCACT
    GGCGCCATCG TGGGCATCCT CGTCGTGACC TTCGTCCTGC TCCTGGTGGC CGTGGACGTC
    ACCTGCTACT TCCTGAACAA GTGTGGCCTG CTCATGTGCA TTGCCGTCAA CCTGTGTGGC
    AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC ATGGAGGAGG GCAAAGCTGC CTTCTCGAAA
    GACGAGTCCA AGGAGCCTAT CGTGGAGGTG CGAACGGAGG AGGAGCGGAC CCCGAATCAC
    GACGGAGGGA AACACACAGA GCCGAACGAG ACCACACCGC TGACGGAGCC CGAGAAGGGT
    CCCGTAGAAG CAAAGCCGGA GACAGAAACA AAGCCAGCGC CAGCCGAAGT CCAGACGGTC
    CCCAACGATG CCACACAAAT AAAGGAGAAT GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu26735
    Clone ID Related Accession (Same CDS sequence) XM_006072491.1
    Accession Version XM_006072491.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785492.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGCGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGCTC TCCTCCGAGT GGAAACCAGA GATCAGGCTC
    CCGTCTGGCA GTGACCACGT CATGCTCAAG TCCCTGGACT GGAATGCCGA GTACGAGGTC
    TATGTGGTGG CCGAGAACCA GCAGGGCAAG TCCAAGGCGG CACACTTTGT GTTCAGGACC
    TCGGCCCAGC CCACAGCCAT CCCAGCCAAC GGCAGCCCCA CCTCGGGCCT GAGCACTGGC
    GCCATCGTGG GCATCCTCGT CGTGACCTTC GTCCTGCTCC TGGTGGCCGT GGACGTCACC
    TGCTACTTCC TGAACAAGTG TGGCCTGCTC ATGTGCATTG CCGTCAACCT GTGTGGCAAA
    GCCGGGCCCG GAGCCAAGGG CAAGGACATG GAGGAGGGCA AAGCTGCCTT CTCGAAAGAC
    GAGTCCAAGG AGCCTATCGT GGAGGTGCGA ACGGAGGAGG AGCGGACCCC GAATCACGAC
    GGAGGGAAAC ACACAGAGCC GAACGAGACC ACACCGCTGA CGGAGCCCGA GAAGGGTCCC
    GTAGAAGCAA AGCCGGAGAC AGAAACAAAG CCAGCGCCAG CCGAAGTCCA GACGGTCCCC
    AACGATGCCA CACAAATAAA GGAGAATGAG AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072553.1
    CDS280..2841
    Translation

    Target ORF information:

    RefSeq Version XM_006072491.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072491.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGCGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGCTC TCCTCCGAGT GGAAACCAGA GATCAGGCTC
    CCGTCTGGCA GTGACCACGT CATGCTCAAG TCCCTGGACT GGAATGCCGA GTACGAGGTC
    TATGTGGTGG CCGAGAACCA GCAGGGCAAG TCCAAGGCGG CACACTTTGT GTTCAGGACC
    TCGGCCCAGC CCACAGCCAT CCCAGCCAAC GGCAGCCCCA CCTCGGGCCT GAGCACTGGC
    GCCATCGTGG GCATCCTCGT CGTGACCTTC GTCCTGCTCC TGGTGGCCGT GGACGTCACC
    TGCTACTTCC TGAACAAGTG TGGCCTGCTC ATGTGCATTG CCGTCAACCT GTGTGGCAAA
    GCCGGGCCCG GAGCCAAGGG CAAGGACATG GAGGAGGGCA AAGCTGCCTT CTCGAAAGAC
    GAGTCCAAGG AGCCTATCGT GGAGGTGCGA ACGGAGGAGG AGCGGACCCC GAATCACGAC
    GGAGGGAAAC ACACAGAGCC GAACGAGACC ACACCGCTGA CGGAGCCCGA GAAGGGTCCC
    GTAGAAGCAA AGCCGGAGAC AGAAACAAAG CCAGCGCCAG CCGAAGTCCA GACGGTCCCC
    AACGATGCCA CACAAATAAA GGAGAATGAG AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu26736
    Clone ID Related Accession (Same CDS sequence) XM_006072492.1
    Accession Version XM_006072492.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2535bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785492.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGCGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAGG CGGCACACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCC
    AACGGCAGCC CCACCTCGGG CCTGAGCACT GGCGCCATCG TGGGCATCCT CGTCGTGACC
    TTCGTCCTGC TCCTGGTGGC CGTGGACGTC ACCTGCTACT TCCTGAACAA GTGTGGCCTG
    CTCATGTGCA TTGCCGTCAA CCTGTGTGGC AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC
    ATGGAGGAGG GCAAAGCTGC CTTCTCGAAA GACGAGTCCA AGGAGCCTAT CGTGGAGGTG
    CGAACGGAGG AGGAGCGGAC CCCGAATCAC GACGGAGGGA AACACACAGA GCCGAACGAG
    ACCACACCGC TGACGGAGCC CGAGAAGGGT CCCGTAGAAG CAAAGCCGGA GACAGAAACA
    AAGCCAGCGC CAGCCGAAGT CCAGACGGTC CCCAACGATG CCACACAAAT AAAGGAGAAT
    GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072554.1
    CDS279..2813
    Translation

    Target ORF information:

    RefSeq Version XM_006072492.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072492.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGCGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAGG CGGCACACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCC
    AACGGCAGCC CCACCTCGGG CCTGAGCACT GGCGCCATCG TGGGCATCCT CGTCGTGACC
    TTCGTCCTGC TCCTGGTGGC CGTGGACGTC ACCTGCTACT TCCTGAACAA GTGTGGCCTG
    CTCATGTGCA TTGCCGTCAA CCTGTGTGGC AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC
    ATGGAGGAGG GCAAAGCTGC CTTCTCGAAA GACGAGTCCA AGGAGCCTAT CGTGGAGGTG
    CGAACGGAGG AGGAGCGGAC CCCGAATCAC GACGGAGGGA AACACACAGA GCCGAACGAG
    ACCACACCGC TGACGGAGCC CGAGAAGGGT CCCGTAGAAG CAAAGCCGGA GACAGAAACA
    AAGCCAGCGC CAGCCGAAGT CCAGACGGTC CCCAACGATG CCACACAAAT AAAGGAGAAT
    GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu26737
    Clone ID Related Accession (Same CDS sequence) XM_006072493.1
    Accession Version XM_006072493.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2532bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785492.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGCGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAGGCGG CACACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCCAAC
    GGCAGCCCCA CCTCGGGCCT GAGCACTGGC GCCATCGTGG GCATCCTCGT CGTGACCTTC
    GTCCTGCTCC TGGTGGCCGT GGACGTCACC TGCTACTTCC TGAACAAGTG TGGCCTGCTC
    ATGTGCATTG CCGTCAACCT GTGTGGCAAA GCCGGGCCCG GAGCCAAGGG CAAGGACATG
    GAGGAGGGCA AAGCTGCCTT CTCGAAAGAC GAGTCCAAGG AGCCTATCGT GGAGGTGCGA
    ACGGAGGAGG AGCGGACCCC GAATCACGAC GGAGGGAAAC ACACAGAGCC GAACGAGACC
    ACACCGCTGA CGGAGCCCGA GAAGGGTCCC GTAGAAGCAA AGCCGGAGAC AGAAACAAAG
    CCAGCGCCAG CCGAAGTCCA GACGGTCCCC AACGATGCCA CACAAATAAA GGAGAATGAG
    AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072555.1
    CDS279..2810
    Translation

    Target ORF information:

    RefSeq Version XM_006072493.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072493.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGCGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAGGCGG CACACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCCAAC
    GGCAGCCCCA CCTCGGGCCT GAGCACTGGC GCCATCGTGG GCATCCTCGT CGTGACCTTC
    GTCCTGCTCC TGGTGGCCGT GGACGTCACC TGCTACTTCC TGAACAAGTG TGGCCTGCTC
    ATGTGCATTG CCGTCAACCT GTGTGGCAAA GCCGGGCCCG GAGCCAAGGG CAAGGACATG
    GAGGAGGGCA AAGCTGCCTT CTCGAAAGAC GAGTCCAAGG AGCCTATCGT GGAGGTGCGA
    ACGGAGGAGG AGCGGACCCC GAATCACGAC GGAGGGAAAC ACACAGAGCC GAACGAGACC
    ACACCGCTGA CGGAGCCCGA GAAGGGTCCC GTAGAAGCAA AGCCGGAGAC AGAAACAAAG
    CCAGCGCCAG CCGAAGTCCA GACGGTCCCC AACGATGCCA CACAAATAAA GGAGAATGAG
    AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu26738
    Clone ID Related Accession (Same CDS sequence) XM_006072494.1
    Accession Version XM_006072494.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785492.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCTTC CTGCTCTTAC AACCACGGAA
    GCAACTAAAG GGGAACCCAG CGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGAAGCT CTCCTCCGAG TGGAAACCAG AGATCAGGCT CCCGTCTGGC
    AGTGACCACG TCATGCTCAA GTCCCTGGAC TGGAATGCCG AGTACGAGGT CTATGTGGTG
    GCCGAGAACC AGCAGGGCAA GTCCAAGGCG GCACACTTTG TGTTCAGGAC CTCGGCCCAG
    CCCACAGCCA TCCCAGCGAC CTTGGGAGGC TCCTCTGCAT CCTACACCTT TGTCTCATTG
    CTTTTCTCTG CAGTGACTCT TCTCTTGCTC TGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072556.1
    CDS281..2596
    Translation

    Target ORF information:

    RefSeq Version XM_006072494.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072494.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCTTC CTGCTCTTAC AACCACGGAA
    GCAACTAAAG GGGAACCCAG CGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGAAGCT CTCCTCCGAG TGGAAACCAG AGATCAGGCT CCCGTCTGGC
    AGTGACCACG TCATGCTCAA GTCCCTGGAC TGGAATGCCG AGTACGAGGT CTATGTGGTG
    GCCGAGAACC AGCAGGGCAA GTCCAAGGCG GCACACTTTG TGTTCAGGAC CTCGGCCCAG
    CCCACAGCCA TCCCAGCGAC CTTGGGAGGC TCCTCTGCAT CCTACACCTT TGTCTCATTG
    CTTTTCTCTG CAGTGACTCT TCTCTTGCTC TGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu26739
    Clone ID Related Accession (Same CDS sequence) XM_006072495.1
    Accession Version XM_006072495.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2283bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785492.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCATAGCCCT
    CTTCCACCGG CTCCTGCTAG CTCTTCCACC CCTGTTCCGT TGTCTCATCT GGATACAACC
    TGGCCTCTTC CTGCTCTTAC AACCACGGAA GCAACTAAAG GGGAACCCAG CGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGCTCTC CTCCGAGTGG
    AAACCAGAGA TCAGGCTCCC GTCTGGCAGT GACCACGTCA TGCTCAAGTC CCTGGACTGG
    AATGCCGAGT ACGAGGTCTA TGTGGTGGCC GAGAACCAGC AGGGCAAGTC CAAGGCGGCA
    CACTTTGTGT TCAGGACCTC GGCCCAGCCC ACAGCCATCC CAGCGACCTT GGGAGGCTCC
    TCTGCATCCT ACACCTTTGT CTCATTGCTT TTCTCTGCAG TGACTCTTCT CTTGCTCTGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072557.1
    CDS280..2562
    Translation

    Target ORF information:

    RefSeq Version XM_006072495.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072495.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCATAGCCCT
    CTTCCACCGG CTCCTGCTAG CTCTTCCACC CCTGTTCCGT TGTCTCATCT GGATACAACC
    TGGCCTCTTC CTGCTCTTAC AACCACGGAA GCAACTAAAG GGGAACCCAG CGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGCTCTC CTCCGAGTGG
    AAACCAGAGA TCAGGCTCCC GTCTGGCAGT GACCACGTCA TGCTCAAGTC CCTGGACTGG
    AATGCCGAGT ACGAGGTCTA TGTGGTGGCC GAGAACCAGC AGGGCAAGTC CAAGGCGGCA
    CACTTTGTGT TCAGGACCTC GGCCCAGCCC ACAGCCATCC CAGCGACCTT GGGAGGCTCC
    TCTGCATCCT ACACCTTTGT CTCATTGCTT TTCTCTGCAG TGACTCTTCT CTTGCTCTGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu26740
    Clone ID Related Accession (Same CDS sequence) XM_006072496.1
    Accession Version XM_006072496.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785492.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGCGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAGG CGGCACACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCG
    ACCTTGGGAG GCTCCTCTGC ATCCTACACC TTTGTCTCAT TGCTTTTCTC TGCAGTGACT
    CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072558.1
    CDS279..2456
    Translation

    Target ORF information:

    RefSeq Version XM_006072496.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072496.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGCGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAGG CGGCACACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCG
    ACCTTGGGAG GCTCCTCTGC ATCCTACACC TTTGTCTCAT TGCTTTTCTC TGCAGTGACT
    CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50839
    Clone ID Related Accession (Same CDS sequence) XM_006072489.2
    Accession Version XM_006072489.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2673bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006072489.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCTTC CTGCTCTTAC AACCACGGAA
    GCAACTAAAG GGGAACCCAG CGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGAAGCT CTCCTCCGAG TGGAAACCAG AGATCAGGCT CCCGTCTGGC
    AGTGACCACG TCATGCTCAA GTCCCTGGAC TGGAATGCCG AGTACGAGGT CTATGTGGTG
    GCCGAGAACC AGCAGGGCAA GTCCAAGGCG GCACACTTTG TGTTCAGGAC CTCGGCCCAG
    CCCACAGCCA TCCCAGCCAA CGGCAGCCCC ACCTCGGGCC TGAGCACTGG CGCCATCGTG
    GGCATCCTCG TCGTGACCTT CGTCCTGCTC CTGGTGGCCG TGGACGTCAC CTGCTACTTC
    CTGAACAAGT GTGGCCTGCT CATGTGCATT GCCGTCAACC TGTGTGGCAA AGCCGGGCCC
    GGAGCCAAGG GCAAGGACAT GGAGGAGGGC AAAGCTGCCT TCTCGAAAGA CGAGTCCAAG
    GAGCCTATCG TGGAGGTGCG AACGGAGGAG GAAAGGACCC CGAATCACGA CGGAGGGAAA
    CACACAGAGC CGAACGAGAC CACACCGCTG ACGGAGCCCG AGAAGGGTCC CGTAGAAGCA
    AAGCCGGAGA CAGAAACAAA GCCAGCGCCA GCCGAAGTCC AGACGGTCCC CAACGATGCC
    ACACAAATAA AGGAGAATGA GAGCAAAGCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072551.1
    CDS293..2965
    Translation

    Target ORF information:

    RefSeq Version XM_006072489.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072489.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCTTC CTGCTCTTAC AACCACGGAA
    GCAACTAAAG GGGAACCCAG CGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGAAGCT CTCCTCCGAG TGGAAACCAG AGATCAGGCT CCCGTCTGGC
    AGTGACCACG TCATGCTCAA GTCCCTGGAC TGGAATGCCG AGTACGAGGT CTATGTGGTG
    GCCGAGAACC AGCAGGGCAA GTCCAAGGCG GCACACTTTG TGTTCAGGAC CTCGGCCCAG
    CCCACAGCCA TCCCAGCCAA CGGCAGCCCC ACCTCGGGCC TGAGCACTGG CGCCATCGTG
    GGCATCCTCG TCGTGACCTT CGTCCTGCTC CTGGTGGCCG TGGACGTCAC CTGCTACTTC
    CTGAACAAGT GTGGCCTGCT CATGTGCATT GCCGTCAACC TGTGTGGCAA AGCCGGGCCC
    GGAGCCAAGG GCAAGGACAT GGAGGAGGGC AAAGCTGCCT TCTCGAAAGA CGAGTCCAAG
    GAGCCTATCG TGGAGGTGCG AACGGAGGAG GAAAGGACCC CGAATCACGA CGGAGGGAAA
    CACACAGAGC CGAACGAGAC CACACCGCTG ACGGAGCCCG AGAAGGGTCC CGTAGAAGCA
    AAGCCGGAGA CAGAAACAAA GCCAGCGCCA GCCGAAGTCC AGACGGTCCC CAACGATGCC
    ACACAAATAA AGGAGAATGA GAGCAAAGCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50840
    Clone ID Related Accession (Same CDS sequence) XM_006072490.2
    Accession Version XM_006072490.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006072490.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA CCCAGCGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAGG CGGCACACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCC AACGGCAGCC CCACCTCGGG CCTGAGCACT
    GGCGCCATCG TGGGCATCCT CGTCGTGACC TTCGTCCTGC TCCTGGTGGC CGTGGACGTC
    ACCTGCTACT TCCTGAACAA GTGTGGCCTG CTCATGTGCA TTGCCGTCAA CCTGTGTGGC
    AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC ATGGAGGAGG GCAAAGCTGC CTTCTCGAAA
    GACGAGTCCA AGGAGCCTAT CGTGGAGGTG CGAACGGAGG AGGAAAGGAC CCCGAATCAC
    GACGGAGGGA AACACACAGA GCCGAACGAG ACCACACCGC TGACGGAGCC CGAGAAGGGT
    CCCGTAGAAG CAAAGCCGGA GACAGAAACA AAGCCAGCGC CAGCCGAAGT CCAGACGGTC
    CCCAACGATG CCACACAAAT AAAGGAGAAT GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072552.1
    CDS292..2856
    Translation

    Target ORF information:

    RefSeq Version XM_006072490.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072490.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA CCCAGCGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAGG CGGCACACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCC AACGGCAGCC CCACCTCGGG CCTGAGCACT
    GGCGCCATCG TGGGCATCCT CGTCGTGACC TTCGTCCTGC TCCTGGTGGC CGTGGACGTC
    ACCTGCTACT TCCTGAACAA GTGTGGCCTG CTCATGTGCA TTGCCGTCAA CCTGTGTGGC
    AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC ATGGAGGAGG GCAAAGCTGC CTTCTCGAAA
    GACGAGTCCA AGGAGCCTAT CGTGGAGGTG CGAACGGAGG AGGAAAGGAC CCCGAATCAC
    GACGGAGGGA AACACACAGA GCCGAACGAG ACCACACCGC TGACGGAGCC CGAGAAGGGT
    CCCGTAGAAG CAAAGCCGGA GACAGAAACA AAGCCAGCGC CAGCCGAAGT CCAGACGGTC
    CCCAACGATG CCACACAAAT AAAGGAGAAT GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50841
    Clone ID Related Accession (Same CDS sequence) XM_006072491.2
    Accession Version XM_006072491.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006072491.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGCGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGCTC TCCTCCGAGT GGAAACCAGA GATCAGGCTC
    CCGTCTGGCA GTGACCACGT CATGCTCAAG TCCCTGGACT GGAATGCCGA GTACGAGGTC
    TATGTGGTGG CCGAGAACCA GCAGGGCAAG TCCAAGGCGG CACACTTTGT GTTCAGGACC
    TCGGCCCAGC CCACAGCCAT CCCAGCCAAC GGCAGCCCCA CCTCGGGCCT GAGCACTGGC
    GCCATCGTGG GCATCCTCGT CGTGACCTTC GTCCTGCTCC TGGTGGCCGT GGACGTCACC
    TGCTACTTCC TGAACAAGTG TGGCCTGCTC ATGTGCATTG CCGTCAACCT GTGTGGCAAA
    GCCGGGCCCG GAGCCAAGGG CAAGGACATG GAGGAGGGCA AAGCTGCCTT CTCGAAAGAC
    GAGTCCAAGG AGCCTATCGT GGAGGTGCGA ACGGAGGAGG AAAGGACCCC GAATCACGAC
    GGAGGGAAAC ACACAGAGCC GAACGAGACC ACACCGCTGA CGGAGCCCGA GAAGGGTCCC
    GTAGAAGCAA AGCCGGAGAC AGAAACAAAG CCAGCGCCAG CCGAAGTCCA GACGGTCCCC
    AACGATGCCA CACAAATAAA GGAGAATGAG AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072553.1
    CDS291..2852
    Translation

    Target ORF information:

    RefSeq Version XM_006072491.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072491.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGCGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGCTC TCCTCCGAGT GGAAACCAGA GATCAGGCTC
    CCGTCTGGCA GTGACCACGT CATGCTCAAG TCCCTGGACT GGAATGCCGA GTACGAGGTC
    TATGTGGTGG CCGAGAACCA GCAGGGCAAG TCCAAGGCGG CACACTTTGT GTTCAGGACC
    TCGGCCCAGC CCACAGCCAT CCCAGCCAAC GGCAGCCCCA CCTCGGGCCT GAGCACTGGC
    GCCATCGTGG GCATCCTCGT CGTGACCTTC GTCCTGCTCC TGGTGGCCGT GGACGTCACC
    TGCTACTTCC TGAACAAGTG TGGCCTGCTC ATGTGCATTG CCGTCAACCT GTGTGGCAAA
    GCCGGGCCCG GAGCCAAGGG CAAGGACATG GAGGAGGGCA AAGCTGCCTT CTCGAAAGAC
    GAGTCCAAGG AGCCTATCGT GGAGGTGCGA ACGGAGGAGG AAAGGACCCC GAATCACGAC
    GGAGGGAAAC ACACAGAGCC GAACGAGACC ACACCGCTGA CGGAGCCCGA GAAGGGTCCC
    GTAGAAGCAA AGCCGGAGAC AGAAACAAAG CCAGCGCCAG CCGAAGTCCA GACGGTCCCC
    AACGATGCCA CACAAATAAA GGAGAATGAG AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50842
    Clone ID Related Accession (Same CDS sequence) XM_006072492.2
    Accession Version XM_006072492.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2535bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006072492.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGCGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAGG CGGCACACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCC
    AACGGCAGCC CCACCTCGGG CCTGAGCACT GGCGCCATCG TGGGCATCCT CGTCGTGACC
    TTCGTCCTGC TCCTGGTGGC CGTGGACGTC ACCTGCTACT TCCTGAACAA GTGTGGCCTG
    CTCATGTGCA TTGCCGTCAA CCTGTGTGGC AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC
    ATGGAGGAGG GCAAAGCTGC CTTCTCGAAA GACGAGTCCA AGGAGCCTAT CGTGGAGGTG
    CGAACGGAGG AGGAAAGGAC CCCGAATCAC GACGGAGGGA AACACACAGA GCCGAACGAG
    ACCACACCGC TGACGGAGCC CGAGAAGGGT CCCGTAGAAG CAAAGCCGGA GACAGAAACA
    AAGCCAGCGC CAGCCGAAGT CCAGACGGTC CCCAACGATG CCACACAAAT AAAGGAGAAT
    GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072554.1
    CDS291..2825
    Translation

    Target ORF information:

    RefSeq Version XM_006072492.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072492.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGCGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAGG CGGCACACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCC
    AACGGCAGCC CCACCTCGGG CCTGAGCACT GGCGCCATCG TGGGCATCCT CGTCGTGACC
    TTCGTCCTGC TCCTGGTGGC CGTGGACGTC ACCTGCTACT TCCTGAACAA GTGTGGCCTG
    CTCATGTGCA TTGCCGTCAA CCTGTGTGGC AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC
    ATGGAGGAGG GCAAAGCTGC CTTCTCGAAA GACGAGTCCA AGGAGCCTAT CGTGGAGGTG
    CGAACGGAGG AGGAAAGGAC CCCGAATCAC GACGGAGGGA AACACACAGA GCCGAACGAG
    ACCACACCGC TGACGGAGCC CGAGAAGGGT CCCGTAGAAG CAAAGCCGGA GACAGAAACA
    AAGCCAGCGC CAGCCGAAGT CCAGACGGTC CCCAACGATG CCACACAAAT AAAGGAGAAT
    GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50843
    Clone ID Related Accession (Same CDS sequence) XM_006072493.2
    Accession Version XM_006072493.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2532bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006072493.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGCGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAGGCGG CACACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCCAAC
    GGCAGCCCCA CCTCGGGCCT GAGCACTGGC GCCATCGTGG GCATCCTCGT CGTGACCTTC
    GTCCTGCTCC TGGTGGCCGT GGACGTCACC TGCTACTTCC TGAACAAGTG TGGCCTGCTC
    ATGTGCATTG CCGTCAACCT GTGTGGCAAA GCCGGGCCCG GAGCCAAGGG CAAGGACATG
    GAGGAGGGCA AAGCTGCCTT CTCGAAAGAC GAGTCCAAGG AGCCTATCGT GGAGGTGCGA
    ACGGAGGAGG AAAGGACCCC GAATCACGAC GGAGGGAAAC ACACAGAGCC GAACGAGACC
    ACACCGCTGA CGGAGCCCGA GAAGGGTCCC GTAGAAGCAA AGCCGGAGAC AGAAACAAAG
    CCAGCGCCAG CCGAAGTCCA GACGGTCCCC AACGATGCCA CACAAATAAA GGAGAATGAG
    AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072555.1
    CDS291..2822
    Translation

    Target ORF information:

    RefSeq Version XM_006072493.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072493.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGCGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAGGCGG CACACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCCAAC
    GGCAGCCCCA CCTCGGGCCT GAGCACTGGC GCCATCGTGG GCATCCTCGT CGTGACCTTC
    GTCCTGCTCC TGGTGGCCGT GGACGTCACC TGCTACTTCC TGAACAAGTG TGGCCTGCTC
    ATGTGCATTG CCGTCAACCT GTGTGGCAAA GCCGGGCCCG GAGCCAAGGG CAAGGACATG
    GAGGAGGGCA AAGCTGCCTT CTCGAAAGAC GAGTCCAAGG AGCCTATCGT GGAGGTGCGA
    ACGGAGGAGG AAAGGACCCC GAATCACGAC GGAGGGAAAC ACACAGAGCC GAACGAGACC
    ACACCGCTGA CGGAGCCCGA GAAGGGTCCC GTAGAAGCAA AGCCGGAGAC AGAAACAAAG
    CCAGCGCCAG CCGAAGTCCA GACGGTCCCC AACGATGCCA CACAAATAAA GGAGAATGAG
    AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50844
    Clone ID Related Accession (Same CDS sequence) XM_006072494.2
    Accession Version XM_006072494.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006072494.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCTTC CTGCTCTTAC AACCACGGAA
    GCAACTAAAG GGGAACCCAG CGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGAAGCT CTCCTCCGAG TGGAAACCAG AGATCAGGCT CCCGTCTGGC
    AGTGACCACG TCATGCTCAA GTCCCTGGAC TGGAATGCCG AGTACGAGGT CTATGTGGTG
    GCCGAGAACC AGCAGGGCAA GTCCAAGGCG GCACACTTTG TGTTCAGGAC CTCGGCCCAG
    CCCACAGCCA TCCCAGCGAC CTTGGGAGGC TCCTCTGCAT CCTACACCTT TGTCTCATTG
    CTTTTCTCTG CAGTGACTCT TCTCTTGCTC TGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072556.1
    CDS294..2609
    Translation

    Target ORF information:

    RefSeq Version XM_006072494.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072494.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCTTC CTGCTCTTAC AACCACGGAA
    GCAACTAAAG GGGAACCCAG CGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGAAGCT CTCCTCCGAG TGGAAACCAG AGATCAGGCT CCCGTCTGGC
    AGTGACCACG TCATGCTCAA GTCCCTGGAC TGGAATGCCG AGTACGAGGT CTATGTGGTG
    GCCGAGAACC AGCAGGGCAA GTCCAAGGCG GCACACTTTG TGTTCAGGAC CTCGGCCCAG
    CCCACAGCCA TCCCAGCGAC CTTGGGAGGC TCCTCTGCAT CCTACACCTT TGTCTCATTG
    CTTTTCTCTG CAGTGACTCT TCTCTTGCTC TGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50845
    Clone ID Related Accession (Same CDS sequence) XM_006072495.2
    Accession Version XM_006072495.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2283bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006072495.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCATAGCCCT
    CTTCCACCGG CTCCTGCTAG CTCTTCCACC CCTGTTCCGT TGTCTCATCT GGATACAACC
    TGGCCTCTTC CTGCTCTTAC AACCACGGAA GCAACTAAAG GGGAACCCAG CGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGCTCTC CTCCGAGTGG
    AAACCAGAGA TCAGGCTCCC GTCTGGCAGT GACCACGTCA TGCTCAAGTC CCTGGACTGG
    AATGCCGAGT ACGAGGTCTA TGTGGTGGCC GAGAACCAGC AGGGCAAGTC CAAGGCGGCA
    CACTTTGTGT TCAGGACCTC GGCCCAGCCC ACAGCCATCC CAGCGACCTT GGGAGGCTCC
    TCTGCATCCT ACACCTTTGT CTCATTGCTT TTCTCTGCAG TGACTCTTCT CTTGCTCTGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072557.1
    CDS293..2575
    Translation

    Target ORF information:

    RefSeq Version XM_006072495.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072495.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCATAGCCCT
    CTTCCACCGG CTCCTGCTAG CTCTTCCACC CCTGTTCCGT TGTCTCATCT GGATACAACC
    TGGCCTCTTC CTGCTCTTAC AACCACGGAA GCAACTAAAG GGGAACCCAG CGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGCTCTC CTCCGAGTGG
    AAACCAGAGA TCAGGCTCCC GTCTGGCAGT GACCACGTCA TGCTCAAGTC CCTGGACTGG
    AATGCCGAGT ACGAGGTCTA TGTGGTGGCC GAGAACCAGC AGGGCAAGTC CAAGGCGGCA
    CACTTTGTGT TCAGGACCTC GGCCCAGCCC ACAGCCATCC CAGCGACCTT GGGAGGCTCC
    TCTGCATCCT ACACCTTTGT CTCATTGCTT TTCTCTGCAG TGACTCTTCT CTTGCTCTGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50846
    Clone ID Related Accession (Same CDS sequence) XM_025266888.1
    Accession Version XM_025266888.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGCGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGAAG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAGG CGGCACACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCG ACCTTGGGAG GCTCCTCTGC ATCCTACACC
    TTTGTCTCAT TGCTTTTCTC TGCAGTGACT CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025122673.1
    CDS292..2499
    Translation

    Target ORF information:

    RefSeq Version XM_025266888.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025266888.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGCGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGAAG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAGG CGGCACACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCG ACCTTGGGAG GCTCCTCTGC ATCCTACACC
    TTTGTCTCAT TGCTTTTCTC TGCAGTGACT CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50847
    Clone ID Related Accession (Same CDS sequence) XM_025266889.1
    Accession Version XM_025266889.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGCGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGCTC TCCTCCGAGT GGAAACCAGA GATCAGGCTC
    CCGTCTGGCA GTGACCACGT CATGCTCAAG TCCCTGGACT GGAATGCCGA GTACGAGGTC
    TATGTGGTGG CCGAGAACCA GCAGGGCAAG TCCAAGGCGG CACACTTTGT GTTCAGGACC
    TCGGCCCAGC CCACAGCCAT CCCAGCGACC TTGGGAGGCT CCTCTGCATC CTACACCTTT
    GTCTCATTGC TTTTCTCTGC AGTGACTCTT CTCTTGCTCT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025122674.1
    CDS291..2495
    Translation

    Target ORF information:

    RefSeq Version XM_025266889.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025266889.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGCGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGCTC TCCTCCGAGT GGAAACCAGA GATCAGGCTC
    CCGTCTGGCA GTGACCACGT CATGCTCAAG TCCCTGGACT GGAATGCCGA GTACGAGGTC
    TATGTGGTGG CCGAGAACCA GCAGGGCAAG TCCAAGGCGG CACACTTTGT GTTCAGGACC
    TCGGCCCAGC CCACAGCCAT CCCAGCGACC TTGGGAGGCT CCTCTGCATC CTACACCTTT
    GTCTCATTGC TTTTCTCTGC AGTGACTCTT CTCTTGCTCT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50848
    Clone ID Related Accession (Same CDS sequence) XM_006072496.2
    Accession Version XM_006072496.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006072496.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGCGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAGG CGGCACACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCG
    ACCTTGGGAG GCTCCTCTGC ATCCTACACC TTTGTCTCAT TGCTTTTCTC TGCAGTGACT
    CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072558.1
    CDS291..2468
    Translation

    Target ORF information:

    RefSeq Version XM_006072496.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072496.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGCGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAGG CGGCACACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCG
    ACCTTGGGAG GCTCCTCTGC ATCCTACACC TTTGTCTCAT TGCTTTTCTC TGCAGTGACT
    CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50850
    Clone ID Related Accession (Same CDS sequence) XM_025266891.1
    Accession Version XM_025266891.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGCGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGAAG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAGG CGGCACACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCG
    ACCTTGGGAG GCTCCTCTGC ATCCTACACC TTTGTCTCAT TGCTTTTCTC TGCAGTGACT
    CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025122676.1
    CDS291..2468
    Translation

    Target ORF information:

    RefSeq Version XM_025266891.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025266891.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGCGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGAAG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAGG CGGCACACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCG
    ACCTTGGGAG GCTCCTCTGC ATCCTACACC TTTGTCTCAT TGCTTTTCTC TGCAGTGACT
    CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50851
    Clone ID Related Accession (Same CDS sequence) XM_025266892.1
    Accession Version XM_025266892.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGCGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAGGCGG CACACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCGACC
    TTGGGAGGCT CCTCTGCATC CTACACCTTT GTCTCATTGC TTTTCTCTGC AGTGACTCTT
    CTCTTGCTCT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025122677.1
    CDS291..2465
    Translation

    Target ORF information:

    RefSeq Version XM_025266892.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025266892.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGCGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAGGCGG CACACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCGACC
    TTGGGAGGCT CCTCTGCATC CTACACCTTT GTCTCATTGC TTTTCTCTGC AGTGACTCTT
    CTCTTGCTCT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu26728
    Clone ID Related Accession (Same CDS sequence) XM_006072484.1
    Accession Version XM_006072484.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785492.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCTTC CTGCTCTTAC AACCACGGAA
    GCAACTAAAG GGGAACCCAG CGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGAAGCT CTCCTCCGAG TGGAAACCAG AGATCAGGCT CCCGTCTGGC
    AGTGACCACG TCATGCTCAA GTCCCTGGAC TGGAATGCCG AGTACGAGGT CTATGTGGTG
    GCCGAGAACC AGCAGGGCAA GTCCAAGGCG GCACACTTTG TGTTCAGGAC CTCGGCCCAG
    CCCACAGCCA TCCCAGCCAA CGGCAGCCCC ACCTCGGGCC TGAGCACTGG CGCCATCGTG
    GGCATCCTCG TCGTGACCTT CGTCCTGCTC CTGGTGGCCG TGGACGTCAC CTGCTACTTC
    CTGAACAAGT GTGGCCTGCT CATGTGCATT GCCGTCAACC TGTGTGGCAA AGCCGGGCCC
    GGAGCCAAGG GCAAGGACAT GGAGGAGGGC AAAGCTGCCT TCTCGAAAGA CGAGTCCAAG
    GAGCCTATCG TGGAGGTGCG AACGGAGGAG GAGCGGACCC CGAATCACGA CGGAGGGAAA
    CACACAGAGC CGAACGAGAC CACACCGCTG ACGGAGCCCG AGCTGCCTGC TGACACCACA
    GCCACTGTGG AGGACATGCT GCCTTCTGTC ACCACCGTCA CCGCTAACTC TGACACTATC
    ACCGAAACCT TTGCCACTGC TCAGAACAGC CCCACCAGTG AGACCACCAC CCTGACCTCC
    AGTATCGCCC CCCCGGTCGC GGCCACCCCT GAGTCAAACT CTGTGCCCGC CGGCCAGGCC
    ACCCCGTCCA AGGGGACCGG CGTCACTGCC GCCTCCCCGG CCCCGGCCCC AGCCCCCAAG
    GTCGCCCCCC TCGTTGACCT GAGCGACCCT CCGGCCTCAG CCCCGGCCGC CAACAACCTG
    TCCTCTAGCG TCCTGGCCAA CCAGGGGGCC GTGCTCAGCC CCAGCACCCC GGCCAGCGCG
    GGCGAGGCCC CCAAGGCCCC TCCAGCCAGC AAGGCCGCCT CCCCGTCGGC AGCCAGTCCT
    CTGGCAGCAG CCCCGACGGC CCCTGCCCCG GAAGCCCCCC AGGCCAAGCA GGAGGCTCCC
    GGCACCAAAG GTCCGGACCC CGAGCCCACC CAGCCCGGCG CCTCGAAGAG CCCACCCGAG
    GCCGCCCGGG CCCTCGCCAG CCCGAAGAGC GAGGCGGCCT CCGTCAGCAC CACAAACCCT
    GCCCAGGGCG AGGACTTTAA AATGGACGAA GGGAACTTCA AGACCCCAGA TATTGACCTT
    GCCAAGGATG TTTTTGCAGC CCTGGGCTCT CCTGCTCCCG CCGCTGGGGC CAGTGGACAA
    GCCTCTGAGC TTGCTCCTTC CACGGCCGAC GGCTCTCTTC CGCCTGCACC AGCCAAGACC
    GAGAAGGGTC CCGTAGAAGC AAAGCCGGAG ACAGAAACAA AGCCAGCGCC AGCCGAAGTC
    CAGACGGTCC CCAACGATGC CACACAAATA AAGGAGAATG AGAGCAAAGC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072546.1
    CDS285..3758
    Translation

    Target ORF information:

    RefSeq Version XM_006072484.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072484.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCTTC CTGCTCTTAC AACCACGGAA
    GCAACTAAAG GGGAACCCAG CGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGAAGCT CTCCTCCGAG TGGAAACCAG AGATCAGGCT CCCGTCTGGC
    AGTGACCACG TCATGCTCAA GTCCCTGGAC TGGAATGCCG AGTACGAGGT CTATGTGGTG
    GCCGAGAACC AGCAGGGCAA GTCCAAGGCG GCACACTTTG TGTTCAGGAC CTCGGCCCAG
    CCCACAGCCA TCCCAGCCAA CGGCAGCCCC ACCTCGGGCC TGAGCACTGG CGCCATCGTG
    GGCATCCTCG TCGTGACCTT CGTCCTGCTC CTGGTGGCCG TGGACGTCAC CTGCTACTTC
    CTGAACAAGT GTGGCCTGCT CATGTGCATT GCCGTCAACC TGTGTGGCAA AGCCGGGCCC
    GGAGCCAAGG GCAAGGACAT GGAGGAGGGC AAAGCTGCCT TCTCGAAAGA CGAGTCCAAG
    GAGCCTATCG TGGAGGTGCG AACGGAGGAG GAGCGGACCC CGAATCACGA CGGAGGGAAA
    CACACAGAGC CGAACGAGAC CACACCGCTG ACGGAGCCCG AGCTGCCTGC TGACACCACA
    GCCACTGTGG AGGACATGCT GCCTTCTGTC ACCACCGTCA CCGCTAACTC TGACACTATC
    ACCGAAACCT TTGCCACTGC TCAGAACAGC CCCACCAGTG AGACCACCAC CCTGACCTCC
    AGTATCGCCC CCCCGGTCGC GGCCACCCCT GAGTCAAACT CTGTGCCCGC CGGCCAGGCC
    ACCCCGTCCA AGGGGACCGG CGTCACTGCC GCCTCCCCGG CCCCGGCCCC AGCCCCCAAG
    GTCGCCCCCC TCGTTGACCT GAGCGACCCT CCGGCCTCAG CCCCGGCCGC CAACAACCTG
    TCCTCTAGCG TCCTGGCCAA CCAGGGGGCC GTGCTCAGCC CCAGCACCCC GGCCAGCGCG
    GGCGAGGCCC CCAAGGCCCC TCCAGCCAGC AAGGCCGCCT CCCCGTCGGC AGCCAGTCCT
    CTGGCAGCAG CCCCGACGGC CCCTGCCCCG GAAGCCCCCC AGGCCAAGCA GGAGGCTCCC
    GGCACCAAAG GTCCGGACCC CGAGCCCACC CAGCCCGGCG CCTCGAAGAG CCCACCCGAG
    GCCGCCCGGG CCCTCGCCAG CCCGAAGAGC GAGGCGGCCT CCGTCAGCAC CACAAACCCT
    GCCCAGGGCG AGGACTTTAA AATGGACGAA GGGAACTTCA AGACCCCAGA TATTGACCTT
    GCCAAGGATG TTTTTGCAGC CCTGGGCTCT CCTGCTCCCG CCGCTGGGGC CAGTGGACAA
    GCCTCTGAGC TTGCTCCTTC CACGGCCGAC GGCTCTCTTC CGCCTGCACC AGCCAAGACC
    GAGAAGGGTC CCGTAGAAGC AAAGCCGGAG ACAGAAACAA AGCCAGCGCC AGCCGAAGTC
    CAGACGGTCC CCAACGATGC CACACAAATA AAGGAGAATG AGAGCAAAGC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu26729
    Clone ID Related Accession (Same CDS sequence) XM_006072485.1
    Accession Version XM_006072485.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3444bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785492.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCATAGCCCT
    CTTCCACCGG CTCCTGCTAG CTCTTCCACC CCTGTTCCGT TGTCTCATCT GGATACAACC
    TGGCCTCTTC CTGCTCTTAC AACCACGGAA GCAACTAAAG GGGAACCCAG CGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGAAGCT CTCCTCCGAG
    TGGAAACCAG AGATCAGGCT CCCGTCTGGC AGTGACCACG TCATGCTCAA GTCCCTGGAC
    TGGAATGCCG AGTACGAGGT CTATGTGGTG GCCGAGAACC AGCAGGGCAA GTCCAAGGCG
    GCACACTTTG TGTTCAGGAC CTCGGCCCAG CCCACAGCCA TCCCAGCCAA CGGCAGCCCC
    ACCTCGGGCC TGAGCACTGG CGCCATCGTG GGCATCCTCG TCGTGACCTT CGTCCTGCTC
    CTGGTGGCCG TGGACGTCAC CTGCTACTTC CTGAACAAGT GTGGCCTGCT CATGTGCATT
    GCCGTCAACC TGTGTGGCAA AGCCGGGCCC GGAGCCAAGG GCAAGGACAT GGAGGAGGGC
    AAAGCTGCCT TCTCGAAAGA CGAGTCCAAG GAGCCTATCG TGGAGGTGCG AACGGAGGAG
    GAGCGGACCC CGAATCACGA CGGAGGGAAA CACACAGAGC CGAACGAGAC CACACCGCTG
    ACGGAGCCCG AGCTGCCTGC TGACACCACA GCCACTGTGG AGGACATGCT GCCTTCTGTC
    ACCACCGTCA CCGCTAACTC TGACACTATC ACCGAAACCT TTGCCACTGC TCAGAACAGC
    CCCACCAGTG AGACCACCAC CCTGACCTCC AGTATCGCCC CCCCGGTCGC GGCCACCCCT
    GAGTCAAACT CTGTGCCCGC CGGCCAGGCC ACCCCGTCCA AGGGGACCGG CGTCACTGCC
    GCCTCCCCGG CCCCGGCCCC AGCCCCCAAG GTCGCCCCCC TCGTTGACCT GAGCGACCCT
    CCGGCCTCAG CCCCGGCCGC CAACAACCTG TCCTCTAGCG TCCTGGCCAA CCAGGGGGCC
    GTGCTCAGCC CCAGCACCCC GGCCAGCGCG GGCGAGGCCC CCAAGGCCCC TCCAGCCAGC
    AAGGCCGCCT CCCCGTCGGC AGCCAGTCCT CTGGCAGCAG CCCCGACGGC CCCTGCCCCG
    GAAGCCCCCC AGGCCAAGCA GGAGGCTCCC GGCACCAAAG GTCCGGACCC CGAGCCCACC
    CAGCCCGGCG CCTCGAAGAG CCCACCCGAG GCCGCCCGGG CCCTCGCCAG CCCGAAGAGC
    GAGGCGGCCT CCGTCAGCAC CACAAACCCT GCCCAGGGCG AGGACTTTAA AATGGACGAA
    GGGAACTTCA AGACCCCAGA TATTGACCTT GCCAAGGATG TTTTTGCAGC CCTGGGCTCT
    CCTGCTCCCG CCGCTGGGGC CAGTGGACAA GCCTCTGAGC TTGCTCCTTC CACGGCCGAC
    GGCTCTCTTC CGCCTGCACC AGCCAAGACC GAGAAGGGTC CCGTAGAAGC AAAGCCGGAG
    ACAGAAACAA AGCCAGCGCC AGCCGAAGTC CAGACGGTCC CCAACGATGC CACACAAATA
    AAGGAGAATG AGAGCAAAGC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072547.1
    CDS285..3728
    Translation

    Target ORF information:

    RefSeq Version XM_006072485.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072485.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCATAGCCCT
    CTTCCACCGG CTCCTGCTAG CTCTTCCACC CCTGTTCCGT TGTCTCATCT GGATACAACC
    TGGCCTCTTC CTGCTCTTAC AACCACGGAA GCAACTAAAG GGGAACCCAG CGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGAAGCT CTCCTCCGAG
    TGGAAACCAG AGATCAGGCT CCCGTCTGGC AGTGACCACG TCATGCTCAA GTCCCTGGAC
    TGGAATGCCG AGTACGAGGT CTATGTGGTG GCCGAGAACC AGCAGGGCAA GTCCAAGGCG
    GCACACTTTG TGTTCAGGAC CTCGGCCCAG CCCACAGCCA TCCCAGCCAA CGGCAGCCCC
    ACCTCGGGCC TGAGCACTGG CGCCATCGTG GGCATCCTCG TCGTGACCTT CGTCCTGCTC
    CTGGTGGCCG TGGACGTCAC CTGCTACTTC CTGAACAAGT GTGGCCTGCT CATGTGCATT
    GCCGTCAACC TGTGTGGCAA AGCCGGGCCC GGAGCCAAGG GCAAGGACAT GGAGGAGGGC
    AAAGCTGCCT TCTCGAAAGA CGAGTCCAAG GAGCCTATCG TGGAGGTGCG AACGGAGGAG
    GAGCGGACCC CGAATCACGA CGGAGGGAAA CACACAGAGC CGAACGAGAC CACACCGCTG
    ACGGAGCCCG AGCTGCCTGC TGACACCACA GCCACTGTGG AGGACATGCT GCCTTCTGTC
    ACCACCGTCA CCGCTAACTC TGACACTATC ACCGAAACCT TTGCCACTGC TCAGAACAGC
    CCCACCAGTG AGACCACCAC CCTGACCTCC AGTATCGCCC CCCCGGTCGC GGCCACCCCT
    GAGTCAAACT CTGTGCCCGC CGGCCAGGCC ACCCCGTCCA AGGGGACCGG CGTCACTGCC
    GCCTCCCCGG CCCCGGCCCC AGCCCCCAAG GTCGCCCCCC TCGTTGACCT GAGCGACCCT
    CCGGCCTCAG CCCCGGCCGC CAACAACCTG TCCTCTAGCG TCCTGGCCAA CCAGGGGGCC
    GTGCTCAGCC CCAGCACCCC GGCCAGCGCG GGCGAGGCCC CCAAGGCCCC TCCAGCCAGC
    AAGGCCGCCT CCCCGTCGGC AGCCAGTCCT CTGGCAGCAG CCCCGACGGC CCCTGCCCCG
    GAAGCCCCCC AGGCCAAGCA GGAGGCTCCC GGCACCAAAG GTCCGGACCC CGAGCCCACC
    CAGCCCGGCG CCTCGAAGAG CCCACCCGAG GCCGCCCGGG CCCTCGCCAG CCCGAAGAGC
    GAGGCGGCCT CCGTCAGCAC CACAAACCCT GCCCAGGGCG AGGACTTTAA AATGGACGAA
    GGGAACTTCA AGACCCCAGA TATTGACCTT GCCAAGGATG TTTTTGCAGC CCTGGGCTCT
    CCTGCTCCCG CCGCTGGGGC CAGTGGACAA GCCTCTGAGC TTGCTCCTTC CACGGCCGAC
    GGCTCTCTTC CGCCTGCACC AGCCAAGACC GAGAAGGGTC CCGTAGAAGC AAAGCCGGAG
    ACAGAAACAA AGCCAGCGCC AGCCGAAGTC CAGACGGTCC CCAACGATGC CACACAAATA
    AAGGAGAATG AGAGCAAAGC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu26730
    Clone ID Related Accession (Same CDS sequence) XM_006072486.1
    Accession Version XM_006072486.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785492.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAGGGAA CCCAGCGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG AAGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AGGCGGCACA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC GGGCCTGAGC
    ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC
    GTCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGCTCATGT GCATTGCCGT CAACCTGTGT
    GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG GACATGGAGG AGGGCAAAGC TGCCTTCTCG
    AAAGACGAGT CCAAGGAGCC TATCGTGGAG GTGCGAACGG AGGAGGAGCG GACCCCGAAT
    CACGACGGAG GGAAACACAC AGAGCCGAAC GAGACCACAC CGCTGACGGA GCCCGAGCTG
    CCTGCTGACA CCACAGCCAC TGTGGAGGAC ATGCTGCCTT CTGTCACCAC CGTCACCGCT
    AACTCTGACA CTATCACCGA AACCTTTGCC ACTGCTCAGA ACAGCCCCAC CAGTGAGACC
    ACCACCCTGA CCTCCAGTAT CGCCCCCCCG GTCGCGGCCA CCCCTGAGTC AAACTCTGTG
    CCCGCCGGCC AGGCCACCCC GTCCAAGGGG ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG
    GCCCCAGCCC CCAAGGTCGC CCCCCTCGTT GACCTGAGCG ACCCTCCGGC CTCAGCCCCG
    GCCGCCAACA ACCTGTCCTC TAGCGTCCTG GCCAACCAGG GGGCCGTGCT CAGCCCCAGC
    ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG GCCCCTCCAG CCAGCAAGGC CGCCTCCCCG
    TCGGCAGCCA GTCCTCTGGC AGCAGCCCCG ACGGCCCCTG CCCCGGAAGC CCCCCAGGCC
    AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG GACCCCGAGC CCACCCAGCC CGGCGCCTCG
    AAGAGCCCAC CCGAGGCCGC CCGGGCCCTC GCCAGCCCGA AGAGCGAGGC GGCCTCCGTC
    AGCACCACAA ACCCTGCCCA GGGCGAGGAC TTTAAAATGG ACGAAGGGAA CTTCAAGACC
    CCAGATATTG ACCTTGCCAA GGATGTTTTT GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT
    GGGGCCAGTG GACAAGCCTC TGAGCTTGCT CCTTCCACGG CCGACGGCTC TCTTCCGCCT
    GCACCAGCCA AGACCGAGAA GGGTCCCGTA GAAGCAAAGC CGGAGACAGA AACAAAGCCA
    GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC GATGCCACAC AAATAAAGGA GAATGAGAGC
    AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072548.1
    CDS285..3713
    Translation

    Target ORF information:

    RefSeq Version XM_006072486.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072486.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAGGGAA CCCAGCGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG AAGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AGGCGGCACA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC GGGCCTGAGC
    ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC
    GTCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGCTCATGT GCATTGCCGT CAACCTGTGT
    GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG GACATGGAGG AGGGCAAAGC TGCCTTCTCG
    AAAGACGAGT CCAAGGAGCC TATCGTGGAG GTGCGAACGG AGGAGGAGCG GACCCCGAAT
    CACGACGGAG GGAAACACAC AGAGCCGAAC GAGACCACAC CGCTGACGGA GCCCGAGCTG
    CCTGCTGACA CCACAGCCAC TGTGGAGGAC ATGCTGCCTT CTGTCACCAC CGTCACCGCT
    AACTCTGACA CTATCACCGA AACCTTTGCC ACTGCTCAGA ACAGCCCCAC CAGTGAGACC
    ACCACCCTGA CCTCCAGTAT CGCCCCCCCG GTCGCGGCCA CCCCTGAGTC AAACTCTGTG
    CCCGCCGGCC AGGCCACCCC GTCCAAGGGG ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG
    GCCCCAGCCC CCAAGGTCGC CCCCCTCGTT GACCTGAGCG ACCCTCCGGC CTCAGCCCCG
    GCCGCCAACA ACCTGTCCTC TAGCGTCCTG GCCAACCAGG GGGCCGTGCT CAGCCCCAGC
    ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG GCCCCTCCAG CCAGCAAGGC CGCCTCCCCG
    TCGGCAGCCA GTCCTCTGGC AGCAGCCCCG ACGGCCCCTG CCCCGGAAGC CCCCCAGGCC
    AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG GACCCCGAGC CCACCCAGCC CGGCGCCTCG
    AAGAGCCCAC CCGAGGCCGC CCGGGCCCTC GCCAGCCCGA AGAGCGAGGC GGCCTCCGTC
    AGCACCACAA ACCCTGCCCA GGGCGAGGAC TTTAAAATGG ACGAAGGGAA CTTCAAGACC
    CCAGATATTG ACCTTGCCAA GGATGTTTTT GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT
    GGGGCCAGTG GACAAGCCTC TGAGCTTGCT CCTTCCACGG CCGACGGCTC TCTTCCGCCT
    GCACCAGCCA AGACCGAGAA GGGTCCCGTA GAAGCAAAGC CGGAGACAGA AACAAAGCCA
    GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC GATGCCACAC AAATAAAGGA GAATGAGAGC
    AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu26731
    Clone ID Related Accession (Same CDS sequence) XM_006072487.1
    Accession Version XM_006072487.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3369bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785492.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA CCCAGCGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG AAGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AGGCGGCACA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC GGGCCTGAGC
    ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC
    GTCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGCTCATGT GCATTGCCGT CAACCTGTGT
    GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG GACATGGAGG AGGGCAAAGC TGCCTTCTCG
    AAAGACGAGT CCAAGGAGCC TATCGTGGAG GTGCGAACGG AGGAGGAGCG GACCCCGAAT
    CACGACGGAG GGAAACACAC AGAGCCGAAC GAGACCACAC CGCTGACGGA GCCCGAGCTG
    CCTGCTGACA CCACAGCCAC TGTGGAGGAC ATGCTGCCTT CTGTCACCAC CGTCACCGCT
    AACTCTGACA CTATCACCGA AACCTTTGCC ACTGCTCAGA ACAGCCCCAC CAGTGAGACC
    ACCACCCTGA CCTCCAGTAT CGCCCCCCCG GTCGCGGCCA CCCCTGAGTC AAACTCTGTG
    CCCGCCGGCC AGGCCACCCC GTCCAAGGGG ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG
    GCCCCAGCCC CCAAGGTCGC CCCCCTCGTT GACCTGAGCG ACCCTCCGGC CTCAGCCCCG
    GCCGCCAACA ACCTGTCCTC TAGCGTCCTG GCCAACCAGG GGGCCGTGCT CAGCCCCAGC
    ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG GCCCCTCCAG CCAGCAAGGC CGCCTCCCCG
    TCGGCAGCCA GTCCTCTGGC AGCAGCCCCG ACGGCCCCTG CCCCGGAAGC CCCCCAGGCC
    AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG GACCCCGAGC CCACCCAGCC CGGCGCCTCG
    AAGAGCCCAC CCGAGGCCGC CCGGGCCCTC GCCAGCCCGA AGAGCGAGGC GGCCTCCGTC
    AGCACCACAA ACCCTGCCCA GGGCGAGGAC TTTAAAATGG ACGAAGGGAA CTTCAAGACC
    CCAGATATTG ACCTTGCCAA GGATGTTTTT GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT
    GGGGCCAGTG GACAAGCCTC TGAGCTTGCT CCTTCCACGG CCGACGGCTC TCTTCCGCCT
    GCACCAGCCA AGACCGAGAA GGGTCCCGTA GAAGCAAAGC CGGAGACAGA AACAAAGCCA
    GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC GATGCCACAC AAATAAAGGA GAATGAGAGC
    AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072549.1
    CDS285..3653
    Translation

    Target ORF information:

    RefSeq Version XM_006072487.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072487.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA CCCAGCGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG AAGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AGGCGGCACA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC GGGCCTGAGC
    ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC
    GTCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGCTCATGT GCATTGCCGT CAACCTGTGT
    GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG GACATGGAGG AGGGCAAAGC TGCCTTCTCG
    AAAGACGAGT CCAAGGAGCC TATCGTGGAG GTGCGAACGG AGGAGGAGCG GACCCCGAAT
    CACGACGGAG GGAAACACAC AGAGCCGAAC GAGACCACAC CGCTGACGGA GCCCGAGCTG
    CCTGCTGACA CCACAGCCAC TGTGGAGGAC ATGCTGCCTT CTGTCACCAC CGTCACCGCT
    AACTCTGACA CTATCACCGA AACCTTTGCC ACTGCTCAGA ACAGCCCCAC CAGTGAGACC
    ACCACCCTGA CCTCCAGTAT CGCCCCCCCG GTCGCGGCCA CCCCTGAGTC AAACTCTGTG
    CCCGCCGGCC AGGCCACCCC GTCCAAGGGG ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG
    GCCCCAGCCC CCAAGGTCGC CCCCCTCGTT GACCTGAGCG ACCCTCCGGC CTCAGCCCCG
    GCCGCCAACA ACCTGTCCTC TAGCGTCCTG GCCAACCAGG GGGCCGTGCT CAGCCCCAGC
    ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG GCCCCTCCAG CCAGCAAGGC CGCCTCCCCG
    TCGGCAGCCA GTCCTCTGGC AGCAGCCCCG ACGGCCCCTG CCCCGGAAGC CCCCCAGGCC
    AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG GACCCCGAGC CCACCCAGCC CGGCGCCTCG
    AAGAGCCCAC CCGAGGCCGC CCGGGCCCTC GCCAGCCCGA AGAGCGAGGC GGCCTCCGTC
    AGCACCACAA ACCCTGCCCA GGGCGAGGAC TTTAAAATGG ACGAAGGGAA CTTCAAGACC
    CCAGATATTG ACCTTGCCAA GGATGTTTTT GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT
    GGGGCCAGTG GACAAGCCTC TGAGCTTGCT CCTTCCACGG CCGACGGCTC TCTTCCGCCT
    GCACCAGCCA AGACCGAGAA GGGTCCCGTA GAAGCAAAGC CGGAGACAGA AACAAAGCCA
    GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC GATGCCACAC AAATAAAGGA GAATGAGAGC
    AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu26732
    Clone ID Related Accession (Same CDS sequence) XM_006072488.1
    Accession Version XM_006072488.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785492.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGCGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGAAG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAGG CGGCACACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCC AACGGCAGCC CCACCTCGGG CCTGAGCACT
    GGCGCCATCG TGGGCATCCT CGTCGTGACC TTCGTCCTGC TCCTGGTGGC CGTGGACGTC
    ACCTGCTACT TCCTGAACAA GTGTGGCCTG CTCATGTGCA TTGCCGTCAA CCTGTGTGGC
    AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC ATGGAGGAGG GCAAAGCTGC CTTCTCGAAA
    GACGAGTCCA AGGAGCCTAT CGTGGAGGTG CGAACGGAGG AGGAGCGGAC CCCGAATCAC
    GACGGAGGGA AACACACAGA GCCGAACGAG ACCACACCGC TGACGGAGCC CGAGCTGCCT
    GCTGACACCA CAGCCACTGT GGAGGACATG CTGCCTTCTG TCACCACCGT CACCGCTAAC
    TCTGACACTA TCACCGAAAC CTTTGCCACT GCTCAGAACA GCCCCACCAG TGAGACCACC
    ACCCTGACCT CCAGTATCGC CCCCCCGGTC GCGGCCACCC CTGAGTCAAA CTCTGTGCCC
    GCCGGCCAGG CCACCCCGTC CAAGGGGACC GGCGTCACTG CCGCCTCCCC GGCCCCGGCC
    CCAGCCCCCA AGGTCGCCCC CCTCGTTGAC CTGAGCGACC CTCCGGCCTC AGCCCCGGCC
    GCCAACAACC TGTCCTCTAG CGTCCTGGCC AACCAGGGGG CCGTGCTCAG CCCCAGCACC
    CCGGCCAGCG CGGGCGAGGC CCCCAAGGCC CCTCCAGCCA GCAAGGCCGC CTCCCCGTCG
    GCAGCCAGTC CTCTGGCAGC AGCCCCGACG GCCCCTGCCC CGGAAGCCCC CCAGGCCAAG
    CAGGAGGCTC CCGGCACCAA AGGTCCGGAC CCCGAGCCCA CCCAGCCCGG CGCCTCGAAG
    AGCCCACCCG AGGCCGCCCG GGCCCTCGCC AGCCCGAAGA GCGAGGCGGC CTCCGTCAGC
    ACCACAAACC CTGCCCAGGG CGAGGACTTT AAAATGGACG AAGGGAACTT CAAGACCCCA
    GATATTGACC TTGCCAAGGA TGTTTTTGCA GCCCTGGGCT CTCCTGCTCC CGCCGCTGGG
    GCCAGTGGAC AAGCCTCTGA GCTTGCTCCT TCCACGGCCG ACGGCTCTCT TCCGCCTGCA
    CCAGCCAAGA CCGAGAAGGG TCCCGTAGAA GCAAAGCCGG AGACAGAAAC AAAGCCAGCG
    CCAGCCGAAG TCCAGACGGT CCCCAACGAT GCCACACAAA TAAAGGAGAA TGAGAGCAAA
    GCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072550.1
    CDS285..3650
    Translation

    Target ORF information:

    RefSeq Version XM_006072488.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072488.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGCGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGAAG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAGG CGGCACACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCC AACGGCAGCC CCACCTCGGG CCTGAGCACT
    GGCGCCATCG TGGGCATCCT CGTCGTGACC TTCGTCCTGC TCCTGGTGGC CGTGGACGTC
    ACCTGCTACT TCCTGAACAA GTGTGGCCTG CTCATGTGCA TTGCCGTCAA CCTGTGTGGC
    AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC ATGGAGGAGG GCAAAGCTGC CTTCTCGAAA
    GACGAGTCCA AGGAGCCTAT CGTGGAGGTG CGAACGGAGG AGGAGCGGAC CCCGAATCAC
    GACGGAGGGA AACACACAGA GCCGAACGAG ACCACACCGC TGACGGAGCC CGAGCTGCCT
    GCTGACACCA CAGCCACTGT GGAGGACATG CTGCCTTCTG TCACCACCGT CACCGCTAAC
    TCTGACACTA TCACCGAAAC CTTTGCCACT GCTCAGAACA GCCCCACCAG TGAGACCACC
    ACCCTGACCT CCAGTATCGC CCCCCCGGTC GCGGCCACCC CTGAGTCAAA CTCTGTGCCC
    GCCGGCCAGG CCACCCCGTC CAAGGGGACC GGCGTCACTG CCGCCTCCCC GGCCCCGGCC
    CCAGCCCCCA AGGTCGCCCC CCTCGTTGAC CTGAGCGACC CTCCGGCCTC AGCCCCGGCC
    GCCAACAACC TGTCCTCTAG CGTCCTGGCC AACCAGGGGG CCGTGCTCAG CCCCAGCACC
    CCGGCCAGCG CGGGCGAGGC CCCCAAGGCC CCTCCAGCCA GCAAGGCCGC CTCCCCGTCG
    GCAGCCAGTC CTCTGGCAGC AGCCCCGACG GCCCCTGCCC CGGAAGCCCC CCAGGCCAAG
    CAGGAGGCTC CCGGCACCAA AGGTCCGGAC CCCGAGCCCA CCCAGCCCGG CGCCTCGAAG
    AGCCCACCCG AGGCCGCCCG GGCCCTCGCC AGCCCGAAGA GCGAGGCGGC CTCCGTCAGC
    ACCACAAACC CTGCCCAGGG CGAGGACTTT AAAATGGACG AAGGGAACTT CAAGACCCCA
    GATATTGACC TTGCCAAGGA TGTTTTTGCA GCCCTGGGCT CTCCTGCTCC CGCCGCTGGG
    GCCAGTGGAC AAGCCTCTGA GCTTGCTCCT TCCACGGCCG ACGGCTCTCT TCCGCCTGCA
    CCAGCCAAGA CCGAGAAGGG TCCCGTAGAA GCAAAGCCGG AGACAGAAAC AAAGCCAGCG
    CCAGCCGAAG TCCAGACGGT CCCCAACGAT GCCACACAAA TAAAGGAGAA TGAGAGCAAA
    GCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50828
    Clone ID Related Accession (Same CDS sequence) XM_006072484.2
    Accession Version XM_006072484.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006072484.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCTTC CTGCTCTTAC AACCACGGAA
    GCAACTAAAG GGGAACCCAG CGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGAAGCT CTCCTCCGAG TGGAAACCAG AGATCAGGCT CCCGTCTGGC
    AGTGACCACG TCATGCTCAA GTCCCTGGAC TGGAATGCCG AGTACGAGGT CTATGTGGTG
    GCCGAGAACC AGCAGGGCAA GTCCAAGGCG GCACACTTTG TGTTCAGGAC CTCGGCCCAG
    CCCACAGCCA TCCCAGCCAA CGGCAGCCCC ACCTCGGGCC TGAGCACTGG CGCCATCGTG
    GGCATCCTCG TCGTGACCTT CGTCCTGCTC CTGGTGGCCG TGGACGTCAC CTGCTACTTC
    CTGAACAAGT GTGGCCTGCT CATGTGCATT GCCGTCAACC TGTGTGGCAA AGCCGGGCCC
    GGAGCCAAGG GCAAGGACAT GGAGGAGGGC AAAGCTGCCT TCTCGAAAGA CGAGTCCAAG
    GAGCCTATCG TGGAGGTGCG AACGGAGGAG GAAAGGACCC CGAATCACGA CGGAGGGAAA
    CACACAGAGC CGAACGAGAC CACACCGCTG ACGGAGCCCG AGCTGCCTGC TGACACCACA
    GCCACTGTGG AGGACATGCT GCCTTCTGTC ACCACCGTCA CCGCTAACTC TGACACTATC
    ACCGAAACCT TTGCCACTGC TCAGAACAGC CCCACCAGTG AGACCACCAC CCTGACCTCC
    AGTATCGCCC CCCCGGTCGC GGCCACCCCT GAGTCAAACT CTGTGCCCGC CGGCCAGGCC
    ACCCCGTCCA AGGGGACCGG CGTCACTGCC GCCTCCCCGG CCCCGGCCCC AGCCCCCAAG
    GTTGCCCCCC TCGTTGACCT GAGCGACCCT CCGGCCTCAG CCCCGGCCGC CAACAACCTG
    TCCTCTAGCG TCCTGGCCAA CCAGGGGGCC GTGCTCAGCC CCAGCACCCC GGCCAGCGCG
    GGCGAGGCCC CCAAGGCCCC TCCAGCCAGC AAGGCCGCCT CCCCGTCGGC AGCCAGTCCT
    CTGGCAGCAG CCCCGACGGC CCCTGCCCCG GAAGCCCCCC AGGCCAAGCA GGAGGCTCCC
    GGCACCAAAG GTCCGGACCC CGAGCCCACC CAGCCCGGCG CCTCGAAGAG CCCACCCGAG
    GCCGCCCGGG CCCTCGCCAG CCCGAAGAGC GAGGCGGCCT CCGTCAGCAC CACAAACCCT
    GCCCAGGGCG AGGACTTTAA AATGGACGAA GGGAACTTCA AGACCCCAGA TATTGACCTT
    GCCAAGGATG TTTTTGCAGC CCTGGGCTCT CCTGCTCCCG CCGCTGGGGC CAGTGGACAA
    GCCTCTGAGC TTGCTCCTTC CACGGCCGAC GGCTCTCTTC CGCCTGCACC AGCCAAGACC
    GAGAAGGGTC CCGTAGAAGC AAAGCCGGAG ACAGAAACAA AGCCAGCGCC AGCCGAAGTC
    CAGACGGTCC CCAACGATGC CACACAAATA AAGGAGAATG AGAGCAAAGC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072546.1
    CDS300..3773
    Translation

    Target ORF information:

    RefSeq Version XM_006072484.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072484.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCTTC CTGCTCTTAC AACCACGGAA
    GCAACTAAAG GGGAACCCAG CGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGAAGCT CTCCTCCGAG TGGAAACCAG AGATCAGGCT CCCGTCTGGC
    AGTGACCACG TCATGCTCAA GTCCCTGGAC TGGAATGCCG AGTACGAGGT CTATGTGGTG
    GCCGAGAACC AGCAGGGCAA GTCCAAGGCG GCACACTTTG TGTTCAGGAC CTCGGCCCAG
    CCCACAGCCA TCCCAGCCAA CGGCAGCCCC ACCTCGGGCC TGAGCACTGG CGCCATCGTG
    GGCATCCTCG TCGTGACCTT CGTCCTGCTC CTGGTGGCCG TGGACGTCAC CTGCTACTTC
    CTGAACAAGT GTGGCCTGCT CATGTGCATT GCCGTCAACC TGTGTGGCAA AGCCGGGCCC
    GGAGCCAAGG GCAAGGACAT GGAGGAGGGC AAAGCTGCCT TCTCGAAAGA CGAGTCCAAG
    GAGCCTATCG TGGAGGTGCG AACGGAGGAG GAAAGGACCC CGAATCACGA CGGAGGGAAA
    CACACAGAGC CGAACGAGAC CACACCGCTG ACGGAGCCCG AGCTGCCTGC TGACACCACA
    GCCACTGTGG AGGACATGCT GCCTTCTGTC ACCACCGTCA CCGCTAACTC TGACACTATC
    ACCGAAACCT TTGCCACTGC TCAGAACAGC CCCACCAGTG AGACCACCAC CCTGACCTCC
    AGTATCGCCC CCCCGGTCGC GGCCACCCCT GAGTCAAACT CTGTGCCCGC CGGCCAGGCC
    ACCCCGTCCA AGGGGACCGG CGTCACTGCC GCCTCCCCGG CCCCGGCCCC AGCCCCCAAG
    GTTGCCCCCC TCGTTGACCT GAGCGACCCT CCGGCCTCAG CCCCGGCCGC CAACAACCTG
    TCCTCTAGCG TCCTGGCCAA CCAGGGGGCC GTGCTCAGCC CCAGCACCCC GGCCAGCGCG
    GGCGAGGCCC CCAAGGCCCC TCCAGCCAGC AAGGCCGCCT CCCCGTCGGC AGCCAGTCCT
    CTGGCAGCAG CCCCGACGGC CCCTGCCCCG GAAGCCCCCC AGGCCAAGCA GGAGGCTCCC
    GGCACCAAAG GTCCGGACCC CGAGCCCACC CAGCCCGGCG CCTCGAAGAG CCCACCCGAG
    GCCGCCCGGG CCCTCGCCAG CCCGAAGAGC GAGGCGGCCT CCGTCAGCAC CACAAACCCT
    GCCCAGGGCG AGGACTTTAA AATGGACGAA GGGAACTTCA AGACCCCAGA TATTGACCTT
    GCCAAGGATG TTTTTGCAGC CCTGGGCTCT CCTGCTCCCG CCGCTGGGGC CAGTGGACAA
    GCCTCTGAGC TTGCTCCTTC CACGGCCGAC GGCTCTCTTC CGCCTGCACC AGCCAAGACC
    GAGAAGGGTC CCGTAGAAGC AAAGCCGGAG ACAGAAACAA AGCCAGCGCC AGCCGAAGTC
    CAGACGGTCC CCAACGATGC CACACAAATA AAGGAGAATG AGAGCAAAGC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50829
    Clone ID Related Accession (Same CDS sequence) XM_025266882.1
    Accession Version XM_025266882.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCTTC CTGCTCTTAC AACCACGGAA
    GCAACTAGGG AACCCAGCGC ACCTAAGCTC GAAGGGCAGA TGGGAGAGGA TGGAAACTCT
    ATTAAGGTGA AGCTGATCAA GCAAGACGAC GGCGGCTCCC CCATCCGACA CTACCTGGTC
    AAATACCGAG CGAAGCTCTC CTCCGAGTGG AAACCAGAGA TCAGGCTCCC GTCTGGCAGT
    GACCACGTCA TGCTCAAGTC CCTGGACTGG AATGCCGAGT ACGAGGTCTA TGTGGTGGCC
    GAGAACCAGC AGGGCAAGTC CAAGGCGGCA CACTTTGTGT TCAGGACCTC GGCCCAGCCC
    ACAGCCATCC CAGCCAACGG CAGCCCCACC TCGGGCCTGA GCACTGGCGC CATCGTGGGC
    ATCCTCGTCG TGACCTTCGT CCTGCTCCTG GTGGCCGTGG ACGTCACCTG CTACTTCCTG
    AACAAGTGTG GCCTGCTCAT GTGCATTGCC GTCAACCTGT GTGGCAAAGC CGGGCCCGGA
    GCCAAGGGCA AGGACATGGA GGAGGGCAAA GCTGCCTTCT CGAAAGACGA GTCCAAGGAG
    CCTATCGTGG AGGTGCGAAC GGAGGAGGAA AGGACCCCGA ATCACGACGG AGGGAAACAC
    ACAGAGCCGA ACGAGACCAC ACCGCTGACG GAGCCCGAGC TGCCTGCTGA CACCACAGCC
    ACTGTGGAGG ACATGCTGCC TTCTGTCACC ACCGTCACCG CTAACTCTGA CACTATCACC
    GAAACCTTTG CCACTGCTCA GAACAGCCCC ACCAGTGAGA CCACCACCCT GACCTCCAGT
    ATCGCCCCCC CGGTCGCGGC CACCCCTGAG TCAAACTCTG TGCCCGCCGG CCAGGCCACC
    CCGTCCAAGG GGACCGGCGT CACTGCCGCC TCCCCGGCCC CGGCCCCAGC CCCCAAGGTT
    GCCCCCCTCG TTGACCTGAG CGACCCTCCG GCCTCAGCCC CGGCCGCCAA CAACCTGTCC
    TCTAGCGTCC TGGCCAACCA GGGGGCCGTG CTCAGCCCCA GCACCCCGGC CAGCGCGGGC
    GAGGCCCCCA AGGCCCCTCC AGCCAGCAAG GCCGCCTCCC CGTCGGCAGC CAGTCCTCTG
    GCAGCAGCCC CGACGGCCCC TGCCCCGGAA GCCCCCCAGG CCAAGCAGGA GGCTCCCGGC
    ACCAAAGGTC CGGACCCCGA GCCCACCCAG CCCGGCGCCT CGAAGAGCCC ACCCGAGGCC
    GCCCGGGCCC TCGCCAGCCC GAAGAGCGAG GCGGCCTCCG TCAGCACCAC AAACCCTGCC
    CAGGGCGAGG ACTTTAAAAT GGACGAAGGG AACTTCAAGA CCCCAGATAT TGACCTTGCC
    AAGGATGTTT TTGCAGCCCT GGGCTCTCCT GCTCCCGCCG CTGGGGCCAG TGGACAAGCC
    TCTGAGCTTG CTCCTTCCAC GGCCGACGGC TCTCTTCCGC CTGCACCAGC CAAGACCGAG
    AAGGGTCCCG TAGAAGCAAA GCCGGAGACA GAAACAAAGC CAGCGCCAGC CGAAGTCCAG
    ACGGTCCCCA ACGATGCCAC ACAAATAAAG GAGAATGAGA GCAAAGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025122667.1
    CDS300..3770
    Translation

    Target ORF information:

    RefSeq Version XM_025266882.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025266882.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCTTC CTGCTCTTAC AACCACGGAA
    GCAACTAGGG AACCCAGCGC ACCTAAGCTC GAAGGGCAGA TGGGAGAGGA TGGAAACTCT
    ATTAAGGTGA AGCTGATCAA GCAAGACGAC GGCGGCTCCC CCATCCGACA CTACCTGGTC
    AAATACCGAG CGAAGCTCTC CTCCGAGTGG AAACCAGAGA TCAGGCTCCC GTCTGGCAGT
    GACCACGTCA TGCTCAAGTC CCTGGACTGG AATGCCGAGT ACGAGGTCTA TGTGGTGGCC
    GAGAACCAGC AGGGCAAGTC CAAGGCGGCA CACTTTGTGT TCAGGACCTC GGCCCAGCCC
    ACAGCCATCC CAGCCAACGG CAGCCCCACC TCGGGCCTGA GCACTGGCGC CATCGTGGGC
    ATCCTCGTCG TGACCTTCGT CCTGCTCCTG GTGGCCGTGG ACGTCACCTG CTACTTCCTG
    AACAAGTGTG GCCTGCTCAT GTGCATTGCC GTCAACCTGT GTGGCAAAGC CGGGCCCGGA
    GCCAAGGGCA AGGACATGGA GGAGGGCAAA GCTGCCTTCT CGAAAGACGA GTCCAAGGAG
    CCTATCGTGG AGGTGCGAAC GGAGGAGGAA AGGACCCCGA ATCACGACGG AGGGAAACAC
    ACAGAGCCGA ACGAGACCAC ACCGCTGACG GAGCCCGAGC TGCCTGCTGA CACCACAGCC
    ACTGTGGAGG ACATGCTGCC TTCTGTCACC ACCGTCACCG CTAACTCTGA CACTATCACC
    GAAACCTTTG CCACTGCTCA GAACAGCCCC ACCAGTGAGA CCACCACCCT GACCTCCAGT
    ATCGCCCCCC CGGTCGCGGC CACCCCTGAG TCAAACTCTG TGCCCGCCGG CCAGGCCACC
    CCGTCCAAGG GGACCGGCGT CACTGCCGCC TCCCCGGCCC CGGCCCCAGC CCCCAAGGTT
    GCCCCCCTCG TTGACCTGAG CGACCCTCCG GCCTCAGCCC CGGCCGCCAA CAACCTGTCC
    TCTAGCGTCC TGGCCAACCA GGGGGCCGTG CTCAGCCCCA GCACCCCGGC CAGCGCGGGC
    GAGGCCCCCA AGGCCCCTCC AGCCAGCAAG GCCGCCTCCC CGTCGGCAGC CAGTCCTCTG
    GCAGCAGCCC CGACGGCCCC TGCCCCGGAA GCCCCCCAGG CCAAGCAGGA GGCTCCCGGC
    ACCAAAGGTC CGGACCCCGA GCCCACCCAG CCCGGCGCCT CGAAGAGCCC ACCCGAGGCC
    GCCCGGGCCC TCGCCAGCCC GAAGAGCGAG GCGGCCTCCG TCAGCACCAC AAACCCTGCC
    CAGGGCGAGG ACTTTAAAAT GGACGAAGGG AACTTCAAGA CCCCAGATAT TGACCTTGCC
    AAGGATGTTT TTGCAGCCCT GGGCTCTCCT GCTCCCGCCG CTGGGGCCAG TGGACAAGCC
    TCTGAGCTTG CTCCTTCCAC GGCCGACGGC TCTCTTCCGC CTGCACCAGC CAAGACCGAG
    AAGGGTCCCG TAGAAGCAAA GCCGGAGACA GAAACAAAGC CAGCGCCAGC CGAAGTCCAG
    ACGGTCCCCA ACGATGCCAC ACAAATAAAG GAGAATGAGA GCAAAGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50830
    Clone ID Related Accession (Same CDS sequence) XM_025266883.1
    Accession Version XM_025266883.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCTTC CTGCTCTTAC AACCACGGAA
    GCAACTAAAG GGGAACCCAG CGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGCTCTC CTCCGAGTGG AAACCAGAGA TCAGGCTCCC GTCTGGCAGT
    GACCACGTCA TGCTCAAGTC CCTGGACTGG AATGCCGAGT ACGAGGTCTA TGTGGTGGCC
    GAGAACCAGC AGGGCAAGTC CAAGGCGGCA CACTTTGTGT TCAGGACCTC GGCCCAGCCC
    ACAGCCATCC CAGCCAACGG CAGCCCCACC TCGGGCCTGA GCACTGGCGC CATCGTGGGC
    ATCCTCGTCG TGACCTTCGT CCTGCTCCTG GTGGCCGTGG ACGTCACCTG CTACTTCCTG
    AACAAGTGTG GCCTGCTCAT GTGCATTGCC GTCAACCTGT GTGGCAAAGC CGGGCCCGGA
    GCCAAGGGCA AGGACATGGA GGAGGGCAAA GCTGCCTTCT CGAAAGACGA GTCCAAGGAG
    CCTATCGTGG AGGTGCGAAC GGAGGAGGAA AGGACCCCGA ATCACGACGG AGGGAAACAC
    ACAGAGCCGA ACGAGACCAC ACCGCTGACG GAGCCCGAGC TGCCTGCTGA CACCACAGCC
    ACTGTGGAGG ACATGCTGCC TTCTGTCACC ACCGTCACCG CTAACTCTGA CACTATCACC
    GAAACCTTTG CCACTGCTCA GAACAGCCCC ACCAGTGAGA CCACCACCCT GACCTCCAGT
    ATCGCCCCCC CGGTCGCGGC CACCCCTGAG TCAAACTCTG TGCCCGCCGG CCAGGCCACC
    CCGTCCAAGG GGACCGGCGT CACTGCCGCC TCCCCGGCCC CGGCCCCAGC CCCCAAGGTT
    GCCCCCCTCG TTGACCTGAG CGACCCTCCG GCCTCAGCCC CGGCCGCCAA CAACCTGTCC
    TCTAGCGTCC TGGCCAACCA GGGGGCCGTG CTCAGCCCCA GCACCCCGGC CAGCGCGGGC
    GAGGCCCCCA AGGCCCCTCC AGCCAGCAAG GCCGCCTCCC CGTCGGCAGC CAGTCCTCTG
    GCAGCAGCCC CGACGGCCCC TGCCCCGGAA GCCCCCCAGG CCAAGCAGGA GGCTCCCGGC
    ACCAAAGGTC CGGACCCCGA GCCCACCCAG CCCGGCGCCT CGAAGAGCCC ACCCGAGGCC
    GCCCGGGCCC TCGCCAGCCC GAAGAGCGAG GCGGCCTCCG TCAGCACCAC AAACCCTGCC
    CAGGGCGAGG ACTTTAAAAT GGACGAAGGG AACTTCAAGA CCCCAGATAT TGACCTTGCC
    AAGGATGTTT TTGCAGCCCT GGGCTCTCCT GCTCCCGCCG CTGGGGCCAG TGGACAAGCC
    TCTGAGCTTG CTCCTTCCAC GGCCGACGGC TCTCTTCCGC CTGCACCAGC CAAGACCGAG
    AAGGGTCCCG TAGAAGCAAA GCCGGAGACA GAAACAAAGC CAGCGCCAGC CGAAGTCCAG
    ACGGTCCCCA ACGATGCCAC ACAAATAAAG GAGAATGAGA GCAAAGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025122668.1
    CDS300..3770
    Translation

    Target ORF information:

    RefSeq Version XM_025266883.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025266883.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCTTC CTGCTCTTAC AACCACGGAA
    GCAACTAAAG GGGAACCCAG CGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGCTCTC CTCCGAGTGG AAACCAGAGA TCAGGCTCCC GTCTGGCAGT
    GACCACGTCA TGCTCAAGTC CCTGGACTGG AATGCCGAGT ACGAGGTCTA TGTGGTGGCC
    GAGAACCAGC AGGGCAAGTC CAAGGCGGCA CACTTTGTGT TCAGGACCTC GGCCCAGCCC
    ACAGCCATCC CAGCCAACGG CAGCCCCACC TCGGGCCTGA GCACTGGCGC CATCGTGGGC
    ATCCTCGTCG TGACCTTCGT CCTGCTCCTG GTGGCCGTGG ACGTCACCTG CTACTTCCTG
    AACAAGTGTG GCCTGCTCAT GTGCATTGCC GTCAACCTGT GTGGCAAAGC CGGGCCCGGA
    GCCAAGGGCA AGGACATGGA GGAGGGCAAA GCTGCCTTCT CGAAAGACGA GTCCAAGGAG
    CCTATCGTGG AGGTGCGAAC GGAGGAGGAA AGGACCCCGA ATCACGACGG AGGGAAACAC
    ACAGAGCCGA ACGAGACCAC ACCGCTGACG GAGCCCGAGC TGCCTGCTGA CACCACAGCC
    ACTGTGGAGG ACATGCTGCC TTCTGTCACC ACCGTCACCG CTAACTCTGA CACTATCACC
    GAAACCTTTG CCACTGCTCA GAACAGCCCC ACCAGTGAGA CCACCACCCT GACCTCCAGT
    ATCGCCCCCC CGGTCGCGGC CACCCCTGAG TCAAACTCTG TGCCCGCCGG CCAGGCCACC
    CCGTCCAAGG GGACCGGCGT CACTGCCGCC TCCCCGGCCC CGGCCCCAGC CCCCAAGGTT
    GCCCCCCTCG TTGACCTGAG CGACCCTCCG GCCTCAGCCC CGGCCGCCAA CAACCTGTCC
    TCTAGCGTCC TGGCCAACCA GGGGGCCGTG CTCAGCCCCA GCACCCCGGC CAGCGCGGGC
    GAGGCCCCCA AGGCCCCTCC AGCCAGCAAG GCCGCCTCCC CGTCGGCAGC CAGTCCTCTG
    GCAGCAGCCC CGACGGCCCC TGCCCCGGAA GCCCCCCAGG CCAAGCAGGA GGCTCCCGGC
    ACCAAAGGTC CGGACCCCGA GCCCACCCAG CCCGGCGCCT CGAAGAGCCC ACCCGAGGCC
    GCCCGGGCCC TCGCCAGCCC GAAGAGCGAG GCGGCCTCCG TCAGCACCAC AAACCCTGCC
    CAGGGCGAGG ACTTTAAAAT GGACGAAGGG AACTTCAAGA CCCCAGATAT TGACCTTGCC
    AAGGATGTTT TTGCAGCCCT GGGCTCTCCT GCTCCCGCCG CTGGGGCCAG TGGACAAGCC
    TCTGAGCTTG CTCCTTCCAC GGCCGACGGC TCTCTTCCGC CTGCACCAGC CAAGACCGAG
    AAGGGTCCCG TAGAAGCAAA GCCGGAGACA GAAACAAAGC CAGCGCCAGC CGAAGTCCAG
    ACGGTCCCCA ACGATGCCAC ACAAATAAAG GAGAATGAGA GCAAAGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50831
    Clone ID Related Accession (Same CDS sequence) XM_006072485.2
    Accession Version XM_006072485.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3444bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006072485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCATAGCCCT
    CTTCCACCGG CTCCTGCTAG CTCTTCCACC CCTGTTCCGT TGTCTCATCT GGATACAACC
    TGGCCTCTTC CTGCTCTTAC AACCACGGAA GCAACTAAAG GGGAACCCAG CGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGAAGCT CTCCTCCGAG
    TGGAAACCAG AGATCAGGCT CCCGTCTGGC AGTGACCACG TCATGCTCAA GTCCCTGGAC
    TGGAATGCCG AGTACGAGGT CTATGTGGTG GCCGAGAACC AGCAGGGCAA GTCCAAGGCG
    GCACACTTTG TGTTCAGGAC CTCGGCCCAG CCCACAGCCA TCCCAGCCAA CGGCAGCCCC
    ACCTCGGGCC TGAGCACTGG CGCCATCGTG GGCATCCTCG TCGTGACCTT CGTCCTGCTC
    CTGGTGGCCG TGGACGTCAC CTGCTACTTC CTGAACAAGT GTGGCCTGCT CATGTGCATT
    GCCGTCAACC TGTGTGGCAA AGCCGGGCCC GGAGCCAAGG GCAAGGACAT GGAGGAGGGC
    AAAGCTGCCT TCTCGAAAGA CGAGTCCAAG GAGCCTATCG TGGAGGTGCG AACGGAGGAG
    GAAAGGACCC CGAATCACGA CGGAGGGAAA CACACAGAGC CGAACGAGAC CACACCGCTG
    ACGGAGCCCG AGCTGCCTGC TGACACCACA GCCACTGTGG AGGACATGCT GCCTTCTGTC
    ACCACCGTCA CCGCTAACTC TGACACTATC ACCGAAACCT TTGCCACTGC TCAGAACAGC
    CCCACCAGTG AGACCACCAC CCTGACCTCC AGTATCGCCC CCCCGGTCGC GGCCACCCCT
    GAGTCAAACT CTGTGCCCGC CGGCCAGGCC ACCCCGTCCA AGGGGACCGG CGTCACTGCC
    GCCTCCCCGG CCCCGGCCCC AGCCCCCAAG GTTGCCCCCC TCGTTGACCT GAGCGACCCT
    CCGGCCTCAG CCCCGGCCGC CAACAACCTG TCCTCTAGCG TCCTGGCCAA CCAGGGGGCC
    GTGCTCAGCC CCAGCACCCC GGCCAGCGCG GGCGAGGCCC CCAAGGCCCC TCCAGCCAGC
    AAGGCCGCCT CCCCGTCGGC AGCCAGTCCT CTGGCAGCAG CCCCGACGGC CCCTGCCCCG
    GAAGCCCCCC AGGCCAAGCA GGAGGCTCCC GGCACCAAAG GTCCGGACCC CGAGCCCACC
    CAGCCCGGCG CCTCGAAGAG CCCACCCGAG GCCGCCCGGG CCCTCGCCAG CCCGAAGAGC
    GAGGCGGCCT CCGTCAGCAC CACAAACCCT GCCCAGGGCG AGGACTTTAA AATGGACGAA
    GGGAACTTCA AGACCCCAGA TATTGACCTT GCCAAGGATG TTTTTGCAGC CCTGGGCTCT
    CCTGCTCCCG CCGCTGGGGC CAGTGGACAA GCCTCTGAGC TTGCTCCTTC CACGGCCGAC
    GGCTCTCTTC CGCCTGCACC AGCCAAGACC GAGAAGGGTC CCGTAGAAGC AAAGCCGGAG
    ACAGAAACAA AGCCAGCGCC AGCCGAAGTC CAGACGGTCC CCAACGATGC CACACAAATA
    AAGGAGAATG AGAGCAAAGC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072547.1
    CDS300..3743
    Translation

    Target ORF information:

    RefSeq Version XM_006072485.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072485.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCATAGCCCT
    CTTCCACCGG CTCCTGCTAG CTCTTCCACC CCTGTTCCGT TGTCTCATCT GGATACAACC
    TGGCCTCTTC CTGCTCTTAC AACCACGGAA GCAACTAAAG GGGAACCCAG CGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGAAGCT CTCCTCCGAG
    TGGAAACCAG AGATCAGGCT CCCGTCTGGC AGTGACCACG TCATGCTCAA GTCCCTGGAC
    TGGAATGCCG AGTACGAGGT CTATGTGGTG GCCGAGAACC AGCAGGGCAA GTCCAAGGCG
    GCACACTTTG TGTTCAGGAC CTCGGCCCAG CCCACAGCCA TCCCAGCCAA CGGCAGCCCC
    ACCTCGGGCC TGAGCACTGG CGCCATCGTG GGCATCCTCG TCGTGACCTT CGTCCTGCTC
    CTGGTGGCCG TGGACGTCAC CTGCTACTTC CTGAACAAGT GTGGCCTGCT CATGTGCATT
    GCCGTCAACC TGTGTGGCAA AGCCGGGCCC GGAGCCAAGG GCAAGGACAT GGAGGAGGGC
    AAAGCTGCCT TCTCGAAAGA CGAGTCCAAG GAGCCTATCG TGGAGGTGCG AACGGAGGAG
    GAAAGGACCC CGAATCACGA CGGAGGGAAA CACACAGAGC CGAACGAGAC CACACCGCTG
    ACGGAGCCCG AGCTGCCTGC TGACACCACA GCCACTGTGG AGGACATGCT GCCTTCTGTC
    ACCACCGTCA CCGCTAACTC TGACACTATC ACCGAAACCT TTGCCACTGC TCAGAACAGC
    CCCACCAGTG AGACCACCAC CCTGACCTCC AGTATCGCCC CCCCGGTCGC GGCCACCCCT
    GAGTCAAACT CTGTGCCCGC CGGCCAGGCC ACCCCGTCCA AGGGGACCGG CGTCACTGCC
    GCCTCCCCGG CCCCGGCCCC AGCCCCCAAG GTTGCCCCCC TCGTTGACCT GAGCGACCCT
    CCGGCCTCAG CCCCGGCCGC CAACAACCTG TCCTCTAGCG TCCTGGCCAA CCAGGGGGCC
    GTGCTCAGCC CCAGCACCCC GGCCAGCGCG GGCGAGGCCC CCAAGGCCCC TCCAGCCAGC
    AAGGCCGCCT CCCCGTCGGC AGCCAGTCCT CTGGCAGCAG CCCCGACGGC CCCTGCCCCG
    GAAGCCCCCC AGGCCAAGCA GGAGGCTCCC GGCACCAAAG GTCCGGACCC CGAGCCCACC
    CAGCCCGGCG CCTCGAAGAG CCCACCCGAG GCCGCCCGGG CCCTCGCCAG CCCGAAGAGC
    GAGGCGGCCT CCGTCAGCAC CACAAACCCT GCCCAGGGCG AGGACTTTAA AATGGACGAA
    GGGAACTTCA AGACCCCAGA TATTGACCTT GCCAAGGATG TTTTTGCAGC CCTGGGCTCT
    CCTGCTCCCG CCGCTGGGGC CAGTGGACAA GCCTCTGAGC TTGCTCCTTC CACGGCCGAC
    GGCTCTCTTC CGCCTGCACC AGCCAAGACC GAGAAGGGTC CCGTAGAAGC AAAGCCGGAG
    ACAGAAACAA AGCCAGCGCC AGCCGAAGTC CAGACGGTCC CCAACGATGC CACACAAATA
    AAGGAGAATG AGAGCAAAGC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50832
    Clone ID Related Accession (Same CDS sequence) XM_025266884.1
    Accession Version XM_025266884.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3432bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAAAGGG GAACCCAGCG CACCTAAGCT CGAAGGGCAG
    ATGGGAGAGG ATGGAAACTC TATTAAGGTG AAGCTGATCA AGCAAGACGA CGGCGGCTCC
    CCCATCCGAC ACTACCTGGT CAAATACCGA GCGAAGCTCT CCTCCGAGTG GAAACCAGAG
    ATCAGGCTCC CGTCTGGCAG TGACCACGTC ATGCTCAAGT CCCTGGACTG GAATGCCGAG
    TACGAGGTCT ATGTGGTGGC CGAGAACCAG CAGGGCAAGT CCAAGGCGGC ACACTTTGTG
    TTCAGGACCT CGGCCCAGCC CACAGCCATC CCAGCCAACG GCAGCCCCAC CTCGGGCCTG
    AGCACTGGCG CCATCGTGGG CATCCTCGTC GTGACCTTCG TCCTGCTCCT GGTGGCCGTG
    GACGTCACCT GCTACTTCCT GAACAAGTGT GGCCTGCTCA TGTGCATTGC CGTCAACCTG
    TGTGGCAAAG CCGGGCCCGG AGCCAAGGGC AAGGACATGG AGGAGGGCAA AGCTGCCTTC
    TCGAAAGACG AGTCCAAGGA GCCTATCGTG GAGGTGCGAA CGGAGGAGGA AAGGACCCCG
    AATCACGACG GAGGGAAACA CACAGAGCCG AACGAGACCA CACCGCTGAC GGAGCCCGAG
    CTGCCTGCTG ACACCACAGC CACTGTGGAG GACATGCTGC CTTCTGTCAC CACCGTCACC
    GCTAACTCTG ACACTATCAC CGAAACCTTT GCCACTGCTC AGAACAGCCC CACCAGTGAG
    ACCACCACCC TGACCTCCAG TATCGCCCCC CCGGTCGCGG CCACCCCTGA GTCAAACTCT
    GTGCCCGCCG GCCAGGCCAC CCCGTCCAAG GGGACCGGCG TCACTGCCGC CTCCCCGGCC
    CCGGCCCCAG CCCCCAAGGT TGCCCCCCTC GTTGACCTGA GCGACCCTCC GGCCTCAGCC
    CCGGCCGCCA ACAACCTGTC CTCTAGCGTC CTGGCCAACC AGGGGGCCGT GCTCAGCCCC
    AGCACCCCGG CCAGCGCGGG CGAGGCCCCC AAGGCCCCTC CAGCCAGCAA GGCCGCCTCC
    CCGTCGGCAG CCAGTCCTCT GGCAGCAGCC CCGACGGCCC CTGCCCCGGA AGCCCCCCAG
    GCCAAGCAGG AGGCTCCCGG CACCAAAGGT CCGGACCCCG AGCCCACCCA GCCCGGCGCC
    TCGAAGAGCC CACCCGAGGC CGCCCGGGCC CTCGCCAGCC CGAAGAGCGA GGCGGCCTCC
    GTCAGCACCA CAAACCCTGC CCAGGGCGAG GACTTTAAAA TGGACGAAGG GAACTTCAAG
    ACCCCAGATA TTGACCTTGC CAAGGATGTT TTTGCAGCCC TGGGCTCTCC TGCTCCCGCC
    GCTGGGGCCA GTGGACAAGC CTCTGAGCTT GCTCCTTCCA CGGCCGACGG CTCTCTTCCG
    CCTGCACCAG CCAAGACCGA GAAGGGTCCC GTAGAAGCAA AGCCGGAGAC AGAAACAAAG
    CCAGCGCCAG CCGAAGTCCA GACGGTCCCC AACGATGCCA CACAAATAAA GGAGAATGAG
    AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025122669.1
    CDS300..3731
    Translation

    Target ORF information:

    RefSeq Version XM_025266884.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025266884.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAAAGGG GAACCCAGCG CACCTAAGCT CGAAGGGCAG
    ATGGGAGAGG ATGGAAACTC TATTAAGGTG AAGCTGATCA AGCAAGACGA CGGCGGCTCC
    CCCATCCGAC ACTACCTGGT CAAATACCGA GCGAAGCTCT CCTCCGAGTG GAAACCAGAG
    ATCAGGCTCC CGTCTGGCAG TGACCACGTC ATGCTCAAGT CCCTGGACTG GAATGCCGAG
    TACGAGGTCT ATGTGGTGGC CGAGAACCAG CAGGGCAAGT CCAAGGCGGC ACACTTTGTG
    TTCAGGACCT CGGCCCAGCC CACAGCCATC CCAGCCAACG GCAGCCCCAC CTCGGGCCTG
    AGCACTGGCG CCATCGTGGG CATCCTCGTC GTGACCTTCG TCCTGCTCCT GGTGGCCGTG
    GACGTCACCT GCTACTTCCT GAACAAGTGT GGCCTGCTCA TGTGCATTGC CGTCAACCTG
    TGTGGCAAAG CCGGGCCCGG AGCCAAGGGC AAGGACATGG AGGAGGGCAA AGCTGCCTTC
    TCGAAAGACG AGTCCAAGGA GCCTATCGTG GAGGTGCGAA CGGAGGAGGA AAGGACCCCG
    AATCACGACG GAGGGAAACA CACAGAGCCG AACGAGACCA CACCGCTGAC GGAGCCCGAG
    CTGCCTGCTG ACACCACAGC CACTGTGGAG GACATGCTGC CTTCTGTCAC CACCGTCACC
    GCTAACTCTG ACACTATCAC CGAAACCTTT GCCACTGCTC AGAACAGCCC CACCAGTGAG
    ACCACCACCC TGACCTCCAG TATCGCCCCC CCGGTCGCGG CCACCCCTGA GTCAAACTCT
    GTGCCCGCCG GCCAGGCCAC CCCGTCCAAG GGGACCGGCG TCACTGCCGC CTCCCCGGCC
    CCGGCCCCAG CCCCCAAGGT TGCCCCCCTC GTTGACCTGA GCGACCCTCC GGCCTCAGCC
    CCGGCCGCCA ACAACCTGTC CTCTAGCGTC CTGGCCAACC AGGGGGCCGT GCTCAGCCCC
    AGCACCCCGG CCAGCGCGGG CGAGGCCCCC AAGGCCCCTC CAGCCAGCAA GGCCGCCTCC
    CCGTCGGCAG CCAGTCCTCT GGCAGCAGCC CCGACGGCCC CTGCCCCGGA AGCCCCCCAG
    GCCAAGCAGG AGGCTCCCGG CACCAAAGGT CCGGACCCCG AGCCCACCCA GCCCGGCGCC
    TCGAAGAGCC CACCCGAGGC CGCCCGGGCC CTCGCCAGCC CGAAGAGCGA GGCGGCCTCC
    GTCAGCACCA CAAACCCTGC CCAGGGCGAG GACTTTAAAA TGGACGAAGG GAACTTCAAG
    ACCCCAGATA TTGACCTTGC CAAGGATGTT TTTGCAGCCC TGGGCTCTCC TGCTCCCGCC
    GCTGGGGCCA GTGGACAAGC CTCTGAGCTT GCTCCTTCCA CGGCCGACGG CTCTCTTCCG
    CCTGCACCAG CCAAGACCGA GAAGGGTCCC GTAGAAGCAA AGCCGGAGAC AGAAACAAAG
    CCAGCGCCAG CCGAAGTCCA GACGGTCCCC AACGATGCCA CACAAATAAA GGAGAATGAG
    AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50833
    Clone ID Related Accession (Same CDS sequence) XM_006072486.2
    Accession Version XM_006072486.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006072486.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAGGGAA CCCAGCGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG AAGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AGGCGGCACA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC GGGCCTGAGC
    ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC
    GTCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGCTCATGT GCATTGCCGT CAACCTGTGT
    GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG GACATGGAGG AGGGCAAAGC TGCCTTCTCG
    AAAGACGAGT CCAAGGAGCC TATCGTGGAG GTGCGAACGG AGGAGGAAAG GACCCCGAAT
    CACGACGGAG GGAAACACAC AGAGCCGAAC GAGACCACAC CGCTGACGGA GCCCGAGCTG
    CCTGCTGACA CCACAGCCAC TGTGGAGGAC ATGCTGCCTT CTGTCACCAC CGTCACCGCT
    AACTCTGACA CTATCACCGA AACCTTTGCC ACTGCTCAGA ACAGCCCCAC CAGTGAGACC
    ACCACCCTGA CCTCCAGTAT CGCCCCCCCG GTCGCGGCCA CCCCTGAGTC AAACTCTGTG
    CCCGCCGGCC AGGCCACCCC GTCCAAGGGG ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG
    GCCCCAGCCC CCAAGGTTGC CCCCCTCGTT GACCTGAGCG ACCCTCCGGC CTCAGCCCCG
    GCCGCCAACA ACCTGTCCTC TAGCGTCCTG GCCAACCAGG GGGCCGTGCT CAGCCCCAGC
    ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG GCCCCTCCAG CCAGCAAGGC CGCCTCCCCG
    TCGGCAGCCA GTCCTCTGGC AGCAGCCCCG ACGGCCCCTG CCCCGGAAGC CCCCCAGGCC
    AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG GACCCCGAGC CCACCCAGCC CGGCGCCTCG
    AAGAGCCCAC CCGAGGCCGC CCGGGCCCTC GCCAGCCCGA AGAGCGAGGC GGCCTCCGTC
    AGCACCACAA ACCCTGCCCA GGGCGAGGAC TTTAAAATGG ACGAAGGGAA CTTCAAGACC
    CCAGATATTG ACCTTGCCAA GGATGTTTTT GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT
    GGGGCCAGTG GACAAGCCTC TGAGCTTGCT CCTTCCACGG CCGACGGCTC TCTTCCGCCT
    GCACCAGCCA AGACCGAGAA GGGTCCCGTA GAAGCAAAGC CGGAGACAGA AACAAAGCCA
    GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC GATGCCACAC AAATAAAGGA GAATGAGAGC
    AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072548.1
    CDS300..3728
    Translation

    Target ORF information:

    RefSeq Version XM_006072486.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072486.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAGGGAA CCCAGCGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG AAGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AGGCGGCACA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC GGGCCTGAGC
    ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC
    GTCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGCTCATGT GCATTGCCGT CAACCTGTGT
    GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG GACATGGAGG AGGGCAAAGC TGCCTTCTCG
    AAAGACGAGT CCAAGGAGCC TATCGTGGAG GTGCGAACGG AGGAGGAAAG GACCCCGAAT
    CACGACGGAG GGAAACACAC AGAGCCGAAC GAGACCACAC CGCTGACGGA GCCCGAGCTG
    CCTGCTGACA CCACAGCCAC TGTGGAGGAC ATGCTGCCTT CTGTCACCAC CGTCACCGCT
    AACTCTGACA CTATCACCGA AACCTTTGCC ACTGCTCAGA ACAGCCCCAC CAGTGAGACC
    ACCACCCTGA CCTCCAGTAT CGCCCCCCCG GTCGCGGCCA CCCCTGAGTC AAACTCTGTG
    CCCGCCGGCC AGGCCACCCC GTCCAAGGGG ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG
    GCCCCAGCCC CCAAGGTTGC CCCCCTCGTT GACCTGAGCG ACCCTCCGGC CTCAGCCCCG
    GCCGCCAACA ACCTGTCCTC TAGCGTCCTG GCCAACCAGG GGGCCGTGCT CAGCCCCAGC
    ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG GCCCCTCCAG CCAGCAAGGC CGCCTCCCCG
    TCGGCAGCCA GTCCTCTGGC AGCAGCCCCG ACGGCCCCTG CCCCGGAAGC CCCCCAGGCC
    AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG GACCCCGAGC CCACCCAGCC CGGCGCCTCG
    AAGAGCCCAC CCGAGGCCGC CCGGGCCCTC GCCAGCCCGA AGAGCGAGGC GGCCTCCGTC
    AGCACCACAA ACCCTGCCCA GGGCGAGGAC TTTAAAATGG ACGAAGGGAA CTTCAAGACC
    CCAGATATTG ACCTTGCCAA GGATGTTTTT GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT
    GGGGCCAGTG GACAAGCCTC TGAGCTTGCT CCTTCCACGG CCGACGGCTC TCTTCCGCCT
    GCACCAGCCA AGACCGAGAA GGGTCCCGTA GAAGCAAAGC CGGAGACAGA AACAAAGCCA
    GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC GATGCCACAC AAATAAAGGA GAATGAGAGC
    AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50834
    Clone ID Related Accession (Same CDS sequence) XM_025266885.1
    Accession Version XM_025266885.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3384bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACAAG GGGAACCCAG CGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGAAGCT CTCCTCCGAG
    TGGAAACCAG AGATCAGGCT CCCGTCTGGC AGTGACCACG TCATGCTCAA GTCCCTGGAC
    TGGAATGCCG AGTACGAGGT CTATGTGGTG GCCGAGAACC AGCAGGGCAA GTCCAAGGCG
    GCACACTTTG TGTTCAGGAC CTCGGCCCAG CCCACAGCCA TCCCAGCCAA CGGCAGCCCC
    ACCTCGGGCC TGAGCACTGG CGCCATCGTG GGCATCCTCG TCGTGACCTT CGTCCTGCTC
    CTGGTGGCCG TGGACGTCAC CTGCTACTTC CTGAACAAGT GTGGCCTGCT CATGTGCATT
    GCCGTCAACC TGTGTGGCAA AGCCGGGCCC GGAGCCAAGG GCAAGGACAT GGAGGAGGGC
    AAAGCTGCCT TCTCGAAAGA CGAGTCCAAG GAGCCTATCG TGGAGGTGCG AACGGAGGAG
    GAAAGGACCC CGAATCACGA CGGAGGGAAA CACACAGAGC CGAACGAGAC CACACCGCTG
    ACGGAGCCCG AGCTGCCTGC TGACACCACA GCCACTGTGG AGGACATGCT GCCTTCTGTC
    ACCACCGTCA CCGCTAACTC TGACACTATC ACCGAAACCT TTGCCACTGC TCAGAACAGC
    CCCACCAGTG AGACCACCAC CCTGACCTCC AGTATCGCCC CCCCGGTCGC GGCCACCCCT
    GAGTCAAACT CTGTGCCCGC CGGCCAGGCC ACCCCGTCCA AGGGGACCGG CGTCACTGCC
    GCCTCCCCGG CCCCGGCCCC AGCCCCCAAG GTTGCCCCCC TCGTTGACCT GAGCGACCCT
    CCGGCCTCAG CCCCGGCCGC CAACAACCTG TCCTCTAGCG TCCTGGCCAA CCAGGGGGCC
    GTGCTCAGCC CCAGCACCCC GGCCAGCGCG GGCGAGGCCC CCAAGGCCCC TCCAGCCAGC
    AAGGCCGCCT CCCCGTCGGC AGCCAGTCCT CTGGCAGCAG CCCCGACGGC CCCTGCCCCG
    GAAGCCCCCC AGGCCAAGCA GGAGGCTCCC GGCACCAAAG GTCCGGACCC CGAGCCCACC
    CAGCCCGGCG CCTCGAAGAG CCCACCCGAG GCCGCCCGGG CCCTCGCCAG CCCGAAGAGC
    GAGGCGGCCT CCGTCAGCAC CACAAACCCT GCCCAGGGCG AGGACTTTAA AATGGACGAA
    GGGAACTTCA AGACCCCAGA TATTGACCTT GCCAAGGATG TTTTTGCAGC CCTGGGCTCT
    CCTGCTCCCG CCGCTGGGGC CAGTGGACAA GCCTCTGAGC TTGCTCCTTC CACGGCCGAC
    GGCTCTCTTC CGCCTGCACC AGCCAAGACC GAGAAGGGTC CCGTAGAAGC AAAGCCGGAG
    ACAGAAACAA AGCCAGCGCC AGCCGAAGTC CAGACGGTCC CCAACGATGC CACACAAATA
    AAGGAGAATG AGAGCAAAGC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025122670.1
    CDS299..3682
    Translation

    Target ORF information:

    RefSeq Version XM_025266885.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025266885.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACAAG GGGAACCCAG CGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGAAGCT CTCCTCCGAG
    TGGAAACCAG AGATCAGGCT CCCGTCTGGC AGTGACCACG TCATGCTCAA GTCCCTGGAC
    TGGAATGCCG AGTACGAGGT CTATGTGGTG GCCGAGAACC AGCAGGGCAA GTCCAAGGCG
    GCACACTTTG TGTTCAGGAC CTCGGCCCAG CCCACAGCCA TCCCAGCCAA CGGCAGCCCC
    ACCTCGGGCC TGAGCACTGG CGCCATCGTG GGCATCCTCG TCGTGACCTT CGTCCTGCTC
    CTGGTGGCCG TGGACGTCAC CTGCTACTTC CTGAACAAGT GTGGCCTGCT CATGTGCATT
    GCCGTCAACC TGTGTGGCAA AGCCGGGCCC GGAGCCAAGG GCAAGGACAT GGAGGAGGGC
    AAAGCTGCCT TCTCGAAAGA CGAGTCCAAG GAGCCTATCG TGGAGGTGCG AACGGAGGAG
    GAAAGGACCC CGAATCACGA CGGAGGGAAA CACACAGAGC CGAACGAGAC CACACCGCTG
    ACGGAGCCCG AGCTGCCTGC TGACACCACA GCCACTGTGG AGGACATGCT GCCTTCTGTC
    ACCACCGTCA CCGCTAACTC TGACACTATC ACCGAAACCT TTGCCACTGC TCAGAACAGC
    CCCACCAGTG AGACCACCAC CCTGACCTCC AGTATCGCCC CCCCGGTCGC GGCCACCCCT
    GAGTCAAACT CTGTGCCCGC CGGCCAGGCC ACCCCGTCCA AGGGGACCGG CGTCACTGCC
    GCCTCCCCGG CCCCGGCCCC AGCCCCCAAG GTTGCCCCCC TCGTTGACCT GAGCGACCCT
    CCGGCCTCAG CCCCGGCCGC CAACAACCTG TCCTCTAGCG TCCTGGCCAA CCAGGGGGCC
    GTGCTCAGCC CCAGCACCCC GGCCAGCGCG GGCGAGGCCC CCAAGGCCCC TCCAGCCAGC
    AAGGCCGCCT CCCCGTCGGC AGCCAGTCCT CTGGCAGCAG CCCCGACGGC CCCTGCCCCG
    GAAGCCCCCC AGGCCAAGCA GGAGGCTCCC GGCACCAAAG GTCCGGACCC CGAGCCCACC
    CAGCCCGGCG CCTCGAAGAG CCCACCCGAG GCCGCCCGGG CCCTCGCCAG CCCGAAGAGC
    GAGGCGGCCT CCGTCAGCAC CACAAACCCT GCCCAGGGCG AGGACTTTAA AATGGACGAA
    GGGAACTTCA AGACCCCAGA TATTGACCTT GCCAAGGATG TTTTTGCAGC CCTGGGCTCT
    CCTGCTCCCG CCGCTGGGGC CAGTGGACAA GCCTCTGAGC TTGCTCCTTC CACGGCCGAC
    GGCTCTCTTC CGCCTGCACC AGCCAAGACC GAGAAGGGTC CCGTAGAAGC AAAGCCGGAG
    ACAGAAACAA AGCCAGCGCC AGCCGAAGTC CAGACGGTCC CCAACGATGC CACACAAATA
    AAGGAGAATG AGAGCAAAGC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50835
    Clone ID Related Accession (Same CDS sequence) XM_025266886.1
    Accession Version XM_025266886.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACGGG AACCCAGCGC ACCTAAGCTC
    GAAGGGCAGA TGGGAGAGGA TGGAAACTCT ATTAAGGTGA AGCTGATCAA GCAAGACGAC
    GGCGGCTCCC CCATCCGACA CTACCTGGTC AAATACCGAG CGAAGCTCTC CTCCGAGTGG
    AAACCAGAGA TCAGGCTCCC GTCTGGCAGT GACCACGTCA TGCTCAAGTC CCTGGACTGG
    AATGCCGAGT ACGAGGTCTA TGTGGTGGCC GAGAACCAGC AGGGCAAGTC CAAGGCGGCA
    CACTTTGTGT TCAGGACCTC GGCCCAGCCC ACAGCCATCC CAGCCAACGG CAGCCCCACC
    TCGGGCCTGA GCACTGGCGC CATCGTGGGC ATCCTCGTCG TGACCTTCGT CCTGCTCCTG
    GTGGCCGTGG ACGTCACCTG CTACTTCCTG AACAAGTGTG GCCTGCTCAT GTGCATTGCC
    GTCAACCTGT GTGGCAAAGC CGGGCCCGGA GCCAAGGGCA AGGACATGGA GGAGGGCAAA
    GCTGCCTTCT CGAAAGACGA GTCCAAGGAG CCTATCGTGG AGGTGCGAAC GGAGGAGGAA
    AGGACCCCGA ATCACGACGG AGGGAAACAC ACAGAGCCGA ACGAGACCAC ACCGCTGACG
    GAGCCCGAGC TGCCTGCTGA CACCACAGCC ACTGTGGAGG ACATGCTGCC TTCTGTCACC
    ACCGTCACCG CTAACTCTGA CACTATCACC GAAACCTTTG CCACTGCTCA GAACAGCCCC
    ACCAGTGAGA CCACCACCCT GACCTCCAGT ATCGCCCCCC CGGTCGCGGC CACCCCTGAG
    TCAAACTCTG TGCCCGCCGG CCAGGCCACC CCGTCCAAGG GGACCGGCGT CACTGCCGCC
    TCCCCGGCCC CGGCCCCAGC CCCCAAGGTT GCCCCCCTCG TTGACCTGAG CGACCCTCCG
    GCCTCAGCCC CGGCCGCCAA CAACCTGTCC TCTAGCGTCC TGGCCAACCA GGGGGCCGTG
    CTCAGCCCCA GCACCCCGGC CAGCGCGGGC GAGGCCCCCA AGGCCCCTCC AGCCAGCAAG
    GCCGCCTCCC CGTCGGCAGC CAGTCCTCTG GCAGCAGCCC CGACGGCCCC TGCCCCGGAA
    GCCCCCCAGG CCAAGCAGGA GGCTCCCGGC ACCAAAGGTC CGGACCCCGA GCCCACCCAG
    CCCGGCGCCT CGAAGAGCCC ACCCGAGGCC GCCCGGGCCC TCGCCAGCCC GAAGAGCGAG
    GCGGCCTCCG TCAGCACCAC AAACCCTGCC CAGGGCGAGG ACTTTAAAAT GGACGAAGGG
    AACTTCAAGA CCCCAGATAT TGACCTTGCC AAGGATGTTT TTGCAGCCCT GGGCTCTCCT
    GCTCCCGCCG CTGGGGCCAG TGGACAAGCC TCTGAGCTTG CTCCTTCCAC GGCCGACGGC
    TCTCTTCCGC CTGCACCAGC CAAGACCGAG AAGGGTCCCG TAGAAGCAAA GCCGGAGACA
    GAAACAAAGC CAGCGCCAGC CGAAGTCCAG ACGGTCCCCA ACGATGCCAC ACAAATAAAG
    GAGAATGAGA GCAAAGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025122671.1
    CDS299..3679
    Translation

    Target ORF information:

    RefSeq Version XM_025266886.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025266886.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACGGG AACCCAGCGC ACCTAAGCTC
    GAAGGGCAGA TGGGAGAGGA TGGAAACTCT ATTAAGGTGA AGCTGATCAA GCAAGACGAC
    GGCGGCTCCC CCATCCGACA CTACCTGGTC AAATACCGAG CGAAGCTCTC CTCCGAGTGG
    AAACCAGAGA TCAGGCTCCC GTCTGGCAGT GACCACGTCA TGCTCAAGTC CCTGGACTGG
    AATGCCGAGT ACGAGGTCTA TGTGGTGGCC GAGAACCAGC AGGGCAAGTC CAAGGCGGCA
    CACTTTGTGT TCAGGACCTC GGCCCAGCCC ACAGCCATCC CAGCCAACGG CAGCCCCACC
    TCGGGCCTGA GCACTGGCGC CATCGTGGGC ATCCTCGTCG TGACCTTCGT CCTGCTCCTG
    GTGGCCGTGG ACGTCACCTG CTACTTCCTG AACAAGTGTG GCCTGCTCAT GTGCATTGCC
    GTCAACCTGT GTGGCAAAGC CGGGCCCGGA GCCAAGGGCA AGGACATGGA GGAGGGCAAA
    GCTGCCTTCT CGAAAGACGA GTCCAAGGAG CCTATCGTGG AGGTGCGAAC GGAGGAGGAA
    AGGACCCCGA ATCACGACGG AGGGAAACAC ACAGAGCCGA ACGAGACCAC ACCGCTGACG
    GAGCCCGAGC TGCCTGCTGA CACCACAGCC ACTGTGGAGG ACATGCTGCC TTCTGTCACC
    ACCGTCACCG CTAACTCTGA CACTATCACC GAAACCTTTG CCACTGCTCA GAACAGCCCC
    ACCAGTGAGA CCACCACCCT GACCTCCAGT ATCGCCCCCC CGGTCGCGGC CACCCCTGAG
    TCAAACTCTG TGCCCGCCGG CCAGGCCACC CCGTCCAAGG GGACCGGCGT CACTGCCGCC
    TCCCCGGCCC CGGCCCCAGC CCCCAAGGTT GCCCCCCTCG TTGACCTGAG CGACCCTCCG
    GCCTCAGCCC CGGCCGCCAA CAACCTGTCC TCTAGCGTCC TGGCCAACCA GGGGGCCGTG
    CTCAGCCCCA GCACCCCGGC CAGCGCGGGC GAGGCCCCCA AGGCCCCTCC AGCCAGCAAG
    GCCGCCTCCC CGTCGGCAGC CAGTCCTCTG GCAGCAGCCC CGACGGCCCC TGCCCCGGAA
    GCCCCCCAGG CCAAGCAGGA GGCTCCCGGC ACCAAAGGTC CGGACCCCGA GCCCACCCAG
    CCCGGCGCCT CGAAGAGCCC ACCCGAGGCC GCCCGGGCCC TCGCCAGCCC GAAGAGCGAG
    GCGGCCTCCG TCAGCACCAC AAACCCTGCC CAGGGCGAGG ACTTTAAAAT GGACGAAGGG
    AACTTCAAGA CCCCAGATAT TGACCTTGCC AAGGATGTTT TTGCAGCCCT GGGCTCTCCT
    GCTCCCGCCG CTGGGGCCAG TGGACAAGCC TCTGAGCTTG CTCCTTCCAC GGCCGACGGC
    TCTCTTCCGC CTGCACCAGC CAAGACCGAG AAGGGTCCCG TAGAAGCAAA GCCGGAGACA
    GAAACAAAGC CAGCGCCAGC CGAAGTCCAG ACGGTCCCCA ACGATGCCAC ACAAATAAAG
    GAGAATGAGA GCAAAGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50836
    Clone ID Related Accession (Same CDS sequence) XM_006072487.2
    Accession Version XM_006072487.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3369bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006072487.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA CCCAGCGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG AAGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AGGCGGCACA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC GGGCCTGAGC
    ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC
    GTCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGCTCATGT GCATTGCCGT CAACCTGTGT
    GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG GACATGGAGG AGGGCAAAGC TGCCTTCTCG
    AAAGACGAGT CCAAGGAGCC TATCGTGGAG GTGCGAACGG AGGAGGAAAG GACCCCGAAT
    CACGACGGAG GGAAACACAC AGAGCCGAAC GAGACCACAC CGCTGACGGA GCCCGAGCTG
    CCTGCTGACA CCACAGCCAC TGTGGAGGAC ATGCTGCCTT CTGTCACCAC CGTCACCGCT
    AACTCTGACA CTATCACCGA AACCTTTGCC ACTGCTCAGA ACAGCCCCAC CAGTGAGACC
    ACCACCCTGA CCTCCAGTAT CGCCCCCCCG GTCGCGGCCA CCCCTGAGTC AAACTCTGTG
    CCCGCCGGCC AGGCCACCCC GTCCAAGGGG ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG
    GCCCCAGCCC CCAAGGTTGC CCCCCTCGTT GACCTGAGCG ACCCTCCGGC CTCAGCCCCG
    GCCGCCAACA ACCTGTCCTC TAGCGTCCTG GCCAACCAGG GGGCCGTGCT CAGCCCCAGC
    ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG GCCCCTCCAG CCAGCAAGGC CGCCTCCCCG
    TCGGCAGCCA GTCCTCTGGC AGCAGCCCCG ACGGCCCCTG CCCCGGAAGC CCCCCAGGCC
    AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG GACCCCGAGC CCACCCAGCC CGGCGCCTCG
    AAGAGCCCAC CCGAGGCCGC CCGGGCCCTC GCCAGCCCGA AGAGCGAGGC GGCCTCCGTC
    AGCACCACAA ACCCTGCCCA GGGCGAGGAC TTTAAAATGG ACGAAGGGAA CTTCAAGACC
    CCAGATATTG ACCTTGCCAA GGATGTTTTT GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT
    GGGGCCAGTG GACAAGCCTC TGAGCTTGCT CCTTCCACGG CCGACGGCTC TCTTCCGCCT
    GCACCAGCCA AGACCGAGAA GGGTCCCGTA GAAGCAAAGC CGGAGACAGA AACAAAGCCA
    GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC GATGCCACAC AAATAAAGGA GAATGAGAGC
    AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072549.1
    CDS299..3667
    Translation

    Target ORF information:

    RefSeq Version XM_006072487.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072487.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA CCCAGCGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG AAGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AGGCGGCACA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC GGGCCTGAGC
    ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC
    GTCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGCTCATGT GCATTGCCGT CAACCTGTGT
    GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG GACATGGAGG AGGGCAAAGC TGCCTTCTCG
    AAAGACGAGT CCAAGGAGCC TATCGTGGAG GTGCGAACGG AGGAGGAAAG GACCCCGAAT
    CACGACGGAG GGAAACACAC AGAGCCGAAC GAGACCACAC CGCTGACGGA GCCCGAGCTG
    CCTGCTGACA CCACAGCCAC TGTGGAGGAC ATGCTGCCTT CTGTCACCAC CGTCACCGCT
    AACTCTGACA CTATCACCGA AACCTTTGCC ACTGCTCAGA ACAGCCCCAC CAGTGAGACC
    ACCACCCTGA CCTCCAGTAT CGCCCCCCCG GTCGCGGCCA CCCCTGAGTC AAACTCTGTG
    CCCGCCGGCC AGGCCACCCC GTCCAAGGGG ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG
    GCCCCAGCCC CCAAGGTTGC CCCCCTCGTT GACCTGAGCG ACCCTCCGGC CTCAGCCCCG
    GCCGCCAACA ACCTGTCCTC TAGCGTCCTG GCCAACCAGG GGGCCGTGCT CAGCCCCAGC
    ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG GCCCCTCCAG CCAGCAAGGC CGCCTCCCCG
    TCGGCAGCCA GTCCTCTGGC AGCAGCCCCG ACGGCCCCTG CCCCGGAAGC CCCCCAGGCC
    AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG GACCCCGAGC CCACCCAGCC CGGCGCCTCG
    AAGAGCCCAC CCGAGGCCGC CCGGGCCCTC GCCAGCCCGA AGAGCGAGGC GGCCTCCGTC
    AGCACCACAA ACCCTGCCCA GGGCGAGGAC TTTAAAATGG ACGAAGGGAA CTTCAAGACC
    CCAGATATTG ACCTTGCCAA GGATGTTTTT GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT
    GGGGCCAGTG GACAAGCCTC TGAGCTTGCT CCTTCCACGG CCGACGGCTC TCTTCCGCCT
    GCACCAGCCA AGACCGAGAA GGGTCCCGTA GAAGCAAAGC CGGAGACAGA AACAAAGCCA
    GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC GATGCCACAC AAATAAAGGA GAATGAGAGC
    AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50837
    Clone ID Related Accession (Same CDS sequence) XM_006072488.2
    Accession Version XM_006072488.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006072488.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGCGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGAAG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAGG CGGCACACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCC AACGGCAGCC CCACCTCGGG CCTGAGCACT
    GGCGCCATCG TGGGCATCCT CGTCGTGACC TTCGTCCTGC TCCTGGTGGC CGTGGACGTC
    ACCTGCTACT TCCTGAACAA GTGTGGCCTG CTCATGTGCA TTGCCGTCAA CCTGTGTGGC
    AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC ATGGAGGAGG GCAAAGCTGC CTTCTCGAAA
    GACGAGTCCA AGGAGCCTAT CGTGGAGGTG CGAACGGAGG AGGAAAGGAC CCCGAATCAC
    GACGGAGGGA AACACACAGA GCCGAACGAG ACCACACCGC TGACGGAGCC CGAGCTGCCT
    GCTGACACCA CAGCCACTGT GGAGGACATG CTGCCTTCTG TCACCACCGT CACCGCTAAC
    TCTGACACTA TCACCGAAAC CTTTGCCACT GCTCAGAACA GCCCCACCAG TGAGACCACC
    ACCCTGACCT CCAGTATCGC CCCCCCGGTC GCGGCCACCC CTGAGTCAAA CTCTGTGCCC
    GCCGGCCAGG CCACCCCGTC CAAGGGGACC GGCGTCACTG CCGCCTCCCC GGCCCCGGCC
    CCAGCCCCCA AGGTTGCCCC CCTCGTTGAC CTGAGCGACC CTCCGGCCTC AGCCCCGGCC
    GCCAACAACC TGTCCTCTAG CGTCCTGGCC AACCAGGGGG CCGTGCTCAG CCCCAGCACC
    CCGGCCAGCG CGGGCGAGGC CCCCAAGGCC CCTCCAGCCA GCAAGGCCGC CTCCCCGTCG
    GCAGCCAGTC CTCTGGCAGC AGCCCCGACG GCCCCTGCCC CGGAAGCCCC CCAGGCCAAG
    CAGGAGGCTC CCGGCACCAA AGGTCCGGAC CCCGAGCCCA CCCAGCCCGG CGCCTCGAAG
    AGCCCACCCG AGGCCGCCCG GGCCCTCGCC AGCCCGAAGA GCGAGGCGGC CTCCGTCAGC
    ACCACAAACC CTGCCCAGGG CGAGGACTTT AAAATGGACG AAGGGAACTT CAAGACCCCA
    GATATTGACC TTGCCAAGGA TGTTTTTGCA GCCCTGGGCT CTCCTGCTCC CGCCGCTGGG
    GCCAGTGGAC AAGCCTCTGA GCTTGCTCCT TCCACGGCCG ACGGCTCTCT TCCGCCTGCA
    CCAGCCAAGA CCGAGAAGGG TCCCGTAGAA GCAAAGCCGG AGACAGAAAC AAAGCCAGCG
    CCAGCCGAAG TCCAGACGGT CCCCAACGAT GCCACACAAA TAAAGGAGAA TGAGAGCAAA
    GCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006072550.1
    CDS299..3664
    Translation

    Target ORF information:

    RefSeq Version XM_006072488.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006072488.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGCGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGAAG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAGG CGGCACACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCC AACGGCAGCC CCACCTCGGG CCTGAGCACT
    GGCGCCATCG TGGGCATCCT CGTCGTGACC TTCGTCCTGC TCCTGGTGGC CGTGGACGTC
    ACCTGCTACT TCCTGAACAA GTGTGGCCTG CTCATGTGCA TTGCCGTCAA CCTGTGTGGC
    AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC ATGGAGGAGG GCAAAGCTGC CTTCTCGAAA
    GACGAGTCCA AGGAGCCTAT CGTGGAGGTG CGAACGGAGG AGGAAAGGAC CCCGAATCAC
    GACGGAGGGA AACACACAGA GCCGAACGAG ACCACACCGC TGACGGAGCC CGAGCTGCCT
    GCTGACACCA CAGCCACTGT GGAGGACATG CTGCCTTCTG TCACCACCGT CACCGCTAAC
    TCTGACACTA TCACCGAAAC CTTTGCCACT GCTCAGAACA GCCCCACCAG TGAGACCACC
    ACCCTGACCT CCAGTATCGC CCCCCCGGTC GCGGCCACCC CTGAGTCAAA CTCTGTGCCC
    GCCGGCCAGG CCACCCCGTC CAAGGGGACC GGCGTCACTG CCGCCTCCCC GGCCCCGGCC
    CCAGCCCCCA AGGTTGCCCC CCTCGTTGAC CTGAGCGACC CTCCGGCCTC AGCCCCGGCC
    GCCAACAACC TGTCCTCTAG CGTCCTGGCC AACCAGGGGG CCGTGCTCAG CCCCAGCACC
    CCGGCCAGCG CGGGCGAGGC CCCCAAGGCC CCTCCAGCCA GCAAGGCCGC CTCCCCGTCG
    GCAGCCAGTC CTCTGGCAGC AGCCCCGACG GCCCCTGCCC CGGAAGCCCC CCAGGCCAAG
    CAGGAGGCTC CCGGCACCAA AGGTCCGGAC CCCGAGCCCA CCCAGCCCGG CGCCTCGAAG
    AGCCCACCCG AGGCCGCCCG GGCCCTCGCC AGCCCGAAGA GCGAGGCGGC CTCCGTCAGC
    ACCACAAACC CTGCCCAGGG CGAGGACTTT AAAATGGACG AAGGGAACTT CAAGACCCCA
    GATATTGACC TTGCCAAGGA TGTTTTTGCA GCCCTGGGCT CTCCTGCTCC CGCCGCTGGG
    GCCAGTGGAC AAGCCTCTGA GCTTGCTCCT TCCACGGCCG ACGGCTCTCT TCCGCCTGCA
    CCAGCCAAGA CCGAGAAGGG TCCCGTAGAA GCAAAGCCGG AGACAGAAAC AAAGCCAGCG
    CCAGCCGAAG TCCAGACGGT CCCCAACGAT GCCACACAAA TAAAGGAGAA TGAGAGCAAA
    GCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50838
    Clone ID Related Accession (Same CDS sequence) XM_025266887.1
    Accession Version XM_025266887.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGCGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGCTC TCCTCCGAGT GGAAACCAGA GATCAGGCTC
    CCGTCTGGCA GTGACCACGT CATGCTCAAG TCCCTGGACT GGAATGCCGA GTACGAGGTC
    TATGTGGTGG CCGAGAACCA GCAGGGCAAG TCCAAGGCGG CACACTTTGT GTTCAGGACC
    TCGGCCCAGC CCACAGCCAT CCCAGCCAAC GGCAGCCCCA CCTCGGGCCT GAGCACTGGC
    GCCATCGTGG GCATCCTCGT CGTGACCTTC GTCCTGCTCC TGGTGGCCGT GGACGTCACC
    TGCTACTTCC TGAACAAGTG TGGCCTGCTC ATGTGCATTG CCGTCAACCT GTGTGGCAAA
    GCCGGGCCCG GAGCCAAGGG CAAGGACATG GAGGAGGGCA AAGCTGCCTT CTCGAAAGAC
    GAGTCCAAGG AGCCTATCGT GGAGGTGCGA ACGGAGGAGG AAAGGACCCC GAATCACGAC
    GGAGGGAAAC ACACAGAGCC GAACGAGACC ACACCGCTGA CGGAGCCCGA GCTGCCTGCT
    GACACCACAG CCACTGTGGA GGACATGCTG CCTTCTGTCA CCACCGTCAC CGCTAACTCT
    GACACTATCA CCGAAACCTT TGCCACTGCT CAGAACAGCC CCACCAGTGA GACCACCACC
    CTGACCTCCA GTATCGCCCC CCCGGTCGCG GCCACCCCTG AGTCAAACTC TGTGCCCGCC
    GGCCAGGCCA CCCCGTCCAA GGGGACCGGC GTCACTGCCG CCTCCCCGGC CCCGGCCCCA
    GCCCCCAAGG TTGCCCCCCT CGTTGACCTG AGCGACCCTC CGGCCTCAGC CCCGGCCGCC
    AACAACCTGT CCTCTAGCGT CCTGGCCAAC CAGGGGGCCG TGCTCAGCCC CAGCACCCCG
    GCCAGCGCGG GCGAGGCCCC CAAGGCCCCT CCAGCCAGCA AGGCCGCCTC CCCGTCGGCA
    GCCAGTCCTC TGGCAGCAGC CCCGACGGCC CCTGCCCCGG AAGCCCCCCA GGCCAAGCAG
    GAGGCTCCCG GCACCAAAGG TCCGGACCCC GAGCCCACCC AGCCCGGCGC CTCGAAGAGC
    CCACCCGAGG CCGCCCGGGC CCTCGCCAGC CCGAAGAGCG AGGCGGCCTC CGTCAGCACC
    ACAAACCCTG CCCAGGGCGA GGACTTTAAA ATGGACGAAG GGAACTTCAA GACCCCAGAT
    ATTGACCTTG CCAAGGATGT TTTTGCAGCC CTGGGCTCTC CTGCTCCCGC CGCTGGGGCC
    AGTGGACAAG CCTCTGAGCT TGCTCCTTCC ACGGCCGACG GCTCTCTTCC GCCTGCACCA
    GCCAAGACCG AGAAGGGTCC CGTAGAAGCA AAGCCGGAGA CAGAAACAAA GCCAGCGCCA
    GCCGAAGTCC AGACGGTCCC CAACGATGCC ACACAAATAA AGGAGAATGA GAGCAAAGCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025122672.1
    CDS299..3661
    Translation

    Target ORF information:

    RefSeq Version XM_025266887.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025266887.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTACGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCATCTGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA CACCCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGTGGGGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCGATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATCGTC ACCATCGTGG GTCTCAAGCC TGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCC
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGCGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGCTC TCCTCCGAGT GGAAACCAGA GATCAGGCTC
    CCGTCTGGCA GTGACCACGT CATGCTCAAG TCCCTGGACT GGAATGCCGA GTACGAGGTC
    TATGTGGTGG CCGAGAACCA GCAGGGCAAG TCCAAGGCGG CACACTTTGT GTTCAGGACC
    TCGGCCCAGC CCACAGCCAT CCCAGCCAAC GGCAGCCCCA CCTCGGGCCT GAGCACTGGC
    GCCATCGTGG GCATCCTCGT CGTGACCTTC GTCCTGCTCC TGGTGGCCGT GGACGTCACC
    TGCTACTTCC TGAACAAGTG TGGCCTGCTC ATGTGCATTG CCGTCAACCT GTGTGGCAAA
    GCCGGGCCCG GAGCCAAGGG CAAGGACATG GAGGAGGGCA AAGCTGCCTT CTCGAAAGAC
    GAGTCCAAGG AGCCTATCGT GGAGGTGCGA ACGGAGGAGG AAAGGACCCC GAATCACGAC
    GGAGGGAAAC ACACAGAGCC GAACGAGACC ACACCGCTGA CGGAGCCCGA GCTGCCTGCT
    GACACCACAG CCACTGTGGA GGACATGCTG CCTTCTGTCA CCACCGTCAC CGCTAACTCT
    GACACTATCA CCGAAACCTT TGCCACTGCT CAGAACAGCC CCACCAGTGA GACCACCACC
    CTGACCTCCA GTATCGCCCC CCCGGTCGCG GCCACCCCTG AGTCAAACTC TGTGCCCGCC
    GGCCAGGCCA CCCCGTCCAA GGGGACCGGC GTCACTGCCG CCTCCCCGGC CCCGGCCCCA
    GCCCCCAAGG TTGCCCCCCT CGTTGACCTG AGCGACCCTC CGGCCTCAGC CCCGGCCGCC
    AACAACCTGT CCTCTAGCGT CCTGGCCAAC CAGGGGGCCG TGCTCAGCCC CAGCACCCCG
    GCCAGCGCGG GCGAGGCCCC CAAGGCCCCT CCAGCCAGCA AGGCCGCCTC CCCGTCGGCA
    GCCAGTCCTC TGGCAGCAGC CCCGACGGCC CCTGCCCCGG AAGCCCCCCA GGCCAAGCAG
    GAGGCTCCCG GCACCAAAGG TCCGGACCCC GAGCCCACCC AGCCCGGCGC CTCGAAGAGC
    CCACCCGAGG CCGCCCGGGC CCTCGCCAGC CCGAAGAGCG AGGCGGCCTC CGTCAGCACC
    ACAAACCCTG CCCAGGGCGA GGACTTTAAA ATGGACGAAG GGAACTTCAA GACCCCAGAT
    ATTGACCTTG CCAAGGATGT TTTTGCAGCC CTGGGCTCTC CTGCTCCCGC CGCTGGGGCC
    AGTGGACAAG CCTCTGAGCT TGCTCCTTCC ACGGCCGACG GCTCTCTTCC GCCTGCACCA
    GCCAAGACCG AGAAGGGTCC CGTAGAAGCA AAGCCGGAGA CAGAAACAAA GCCAGCGCCA
    GCCGAAGTCC AGACGGTCCC CAACGATGCC ACACAAATAA AGGAGAATGA GAGCAAAGCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu50849
    Clone ID Related Accession (Same CDS sequence) XM_025266890.1
    Accession Version XM_025266890.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3333bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product neural cell adhesion molecule 1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGCGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAGGCGG CACACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCCAAC
    GGCAGCCCCA CCTCGGGCCT GAGCACTGGC GCCATCGTGG GCATCCTCGT CGTGACCTTC
    GTCCTGCTCC TGGTGGCCGT GGACGTCACC TGCTACTTCC TGAACAAGTG TGGCCTGCTC
    ATGTGCATTG CCGTCAACCT GTGTGGCAAA GCCGGGCCCG GAGCCAAGGG CAAGGACATG
    GAGGAGGGCA AAGCTGCCTT CTCGAAAGAC GAGTCCAAGG AGCCTATCGT GGAGGTGCGA
    ACGGAGGAGG AAAGGACCCC GAATCACGAC GGAGGGAAAC ACACAGAGCC GAACGAGACC
    ACACCGCTGA CGGAGCCCGA GCTGCCTGCT GACACCACAG CCACTGTGGA GGACATGCTG
    CCTTCTGTCA CCACCGTCAC CGCTAACTCT GACACTATCA CCGAAACCTT TGCCACTGCT
    CAGAACAGCC CCACCAGTGA GACCACCACC CTGACCTCCA GTATCGCCCC CCCGGTCGCG
    GCCACCCCTG AGTCAAACTC TGTGCCCGCC GGCCAGGCCA CCCCGTCCAA GGGGACCGGC
    GTCACTGCCG CCTCCCCGGC CCCGGCCCCA GCCCCCAAGG TTGCCCCCCT CGTTGACCTG
    AGCGACCCTC CGGCCTCAGC CCCGGCCGCC AACAACCTGT CCTCTAGCGT CCTGGCCAAC
    CAGGGGGCCG TGCTCAGCCC CAGCACCCCG GCCAGCGCGG GCGAGGCCCC CAAGGCCCCT
    CCAGCCAGCA AGGCCGCCTC CCCGTCGGCA GCCAGTCCTC TGGCAGCAGC CCCGACGGCC
    CCTGCCCCGG AAGCCCCCCA GGCCAAGCAG GAGGCTCCCG GCACCAAAGG TCCGGACCCC
    GAGCCCACCC AGCCCGGCGC CTCGAAGAGC CCACCCGAGG CCGCCCGGGC CCTCGCCAGC
    CCGAAGAGCG AGGCGGCCTC CGTCAGCACC ACAAACCCTG CCCAGGGCGA GGACTTTAAA
    ATGGACGAAG GGAACTTCAA GACCCCAGAT ATTGACCTTG CCAAGGATGT TTTTGCAGCC
    CTGGGCTCTC CTGCTCCCGC CGCTGGGGCC AGTGGACAAG CCTCTGAGCT TGCTCCTTCC
    ACGGCCGACG GCTCTCTTCC GCCTGCACCA GCCAAGACCG AGAAGGGTCC CGTAGAAGCA
    AAGCCGGAGA CAGAAACAAA GCCAGCGCCA GCCGAAGTCC AGACGGTCCC CAACGATGCC
    ACACAAATAA AGGAGAATGA GAGCAAAGCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025122675.1
    CDS298..3630
    Translation

    Target ORF information:

    RefSeq Version XM_025266890.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neural cell adhesion molecule 1 (NCAM1), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025266890.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAACGGAGAA
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGCGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAATGCAC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATA
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCATGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGATGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCCGAGT ACGTCTGCAT TGCTGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACGTCCA CCCGAAACAT CAGCAGCGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTACGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCATCTGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA CACCCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGTGGGGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCGATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATCGTC
    ACCATCGTGG GTCTCAAGCC TGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCC GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGCGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAGGCGG CACACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCCAAC
    GGCAGCCCCA CCTCGGGCCT GAGCACTGGC GCCATCGTGG GCATCCTCGT CGTGACCTTC
    GTCCTGCTCC TGGTGGCCGT GGACGTCACC TGCTACTTCC TGAACAAGTG TGGCCTGCTC
    ATGTGCATTG CCGTCAACCT GTGTGGCAAA GCCGGGCCCG GAGCCAAGGG CAAGGACATG
    GAGGAGGGCA AAGCTGCCTT CTCGAAAGAC GAGTCCAAGG AGCCTATCGT GGAGGTGCGA
    ACGGAGGAGG AAAGGACCCC GAATCACGAC GGAGGGAAAC ACACAGAGCC GAACGAGACC
    ACACCGCTGA CGGAGCCCGA GCTGCCTGCT GACACCACAG CCACTGTGGA GGACATGCTG
    CCTTCTGTCA CCACCGTCAC CGCTAACTCT GACACTATCA CCGAAACCTT TGCCACTGCT
    CAGAACAGCC CCACCAGTGA GACCACCACC CTGACCTCCA GTATCGCCCC CCCGGTCGCG
    GCCACCCCTG AGTCAAACTC TGTGCCCGCC GGCCAGGCCA CCCCGTCCAA GGGGACCGGC
    GTCACTGCCG CCTCCCCGGC CCCGGCCCCA GCCCCCAAGG TTGCCCCCCT CGTTGACCTG
    AGCGACCCTC CGGCCTCAGC CCCGGCCGCC AACAACCTGT CCTCTAGCGT CCTGGCCAAC
    CAGGGGGCCG TGCTCAGCCC CAGCACCCCG GCCAGCGCGG GCGAGGCCCC CAAGGCCCCT
    CCAGCCAGCA AGGCCGCCTC CCCGTCGGCA GCCAGTCCTC TGGCAGCAGC CCCGACGGCC
    CCTGCCCCGG AAGCCCCCCA GGCCAAGCAG GAGGCTCCCG GCACCAAAGG TCCGGACCCC
    GAGCCCACCC AGCCCGGCGC CTCGAAGAGC CCACCCGAGG CCGCCCGGGC CCTCGCCAGC
    CCGAAGAGCG AGGCGGCCTC CGTCAGCACC ACAAACCCTG CCCAGGGCGA GGACTTTAAA
    ATGGACGAAG GGAACTTCAA GACCCCAGAT ATTGACCTTG CCAAGGATGT TTTTGCAGCC
    CTGGGCTCTC CTGCTCCCGC CGCTGGGGCC AGTGGACAAG CCTCTGAGCT TGCTCCTTCC
    ACGGCCGACG GCTCTCTTCC GCCTGCACCA GCCAAGACCG AGAAGGGTCC CGTAGAAGCA
    AAGCCGGAGA CAGAAACAAA GCCAGCGCCA GCCGAAGTCC AGACGGTCCC CAACGATGCC
    ACACAAATAA AGGAGAATGA GAGCAAAGCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.