DENND4B gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_025287791.1	XP_025143576.1	DENN domain-containing protein 4B isoform X2
XM_006044144.2	XP_006044206.1	DENN domain-containing protein 4B isoform X4
XM_025287792.1	XP_025143577.1	DENN domain-containing protein 4B isoform X5
XM_006044143.2	XP_006044205.1	DENN domain-containing protein 4B isoform X3
XM_006044143.1	XP_006044205.1	DENN domain-containing protein 4B isoform X2
XM_025287790.1	XP_025143575.1	DENN domain-containing protein 4B isoform X2
XM_006044142.1	XP_006044204.1	DENN domain-containing protein 4B isoform X1
XM_006044144.1	XP_006044206.1	DENN domain-containing protein 4B isoform X3
XM_006044142.2	XP_006044204.1	DENN domain-containing protein 4B isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu03360	XM_006044143.2 Latest version!	Bubalus bubalis DENN domain containing 4B (DENND4B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu03361	XM_006044144.2 Latest version!	Bubalus bubalis DENN domain containing 4B (DENND4B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu68017	XM_025287790.1 Latest version!	Bubalus bubalis DENN domain containing 4B (DENND4B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu68017	XM_025287791.1 Latest version!	Bubalus bubalis DENN domain containing 4B (DENND4B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu68018	XM_025287792.1 Latest version!	Bubalus bubalis DENN domain containing 4B (DENND4B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu03359	XM_006044142.2 Latest version!	Bubalus bubalis DENN domain containing 4B (DENND4B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
View Old Accession Versions >>
OBu03360	XM_006044143.1	Bubalus bubalis DENN/MADD domain containing 4B (DENND4B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu03361	XM_006044144.1	Bubalus bubalis DENN/MADD domain containing 4B (DENND4B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu03359	XM_006044142.1	Bubalus bubalis DENN/MADD domain containing 4B (DENND4B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

DENND4B ( XM_006044143.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044205.1 Bubalus bubalis DENN domain containing 4B (DENND4B), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu03360
Clone ID Related Accession (Same CDS sequence)	XM_006044143.2 , XM_006044143.1
Accession Version	XM_006044143.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	DENN domain-containing protein 4B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006044143.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TCGTGATAGC TGGACTTGCA GGGAACGGAG CACCCATCCC TGAGGAAACA 
TGGATTCCTG AACCCAGTGG ACCCCTGCGC CCTCCTCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAAGGC CCTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCCGCGG GGGGCCACCC CTTGGAACTC AGCGCTGGGC TCCTAGGTGG AACTCAACCT 
GTCATCTGCT ACCGGAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ATACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGCG AGGGCACTCC TCATACTTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCCGTGCC CGTCTTCTCC ACCTTTGTGC TCACGGGTGC AGCTGGTGAT 
AAGGTGTACG GTGCTGCCCT GCAGTTCTAC GAGGCGTTCC CGAGGGCCAG GCTGTCGGAG 
CGGCAGGCTC GGGCCCTGGG CCTGCTGAGT GCTGTGGAGC GGGGCCGGGC ACTGGGGGGC 
CGGGCTGTGC GCAGCCGCCG CGCCATCGCT GTGCTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGCGCCT TCCTCACCTT CCTCTACCGC TACTCCGTCT CGGGCCCCCA CCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AATGTTCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGCCAGCC TGTATCCTCA 
CCCCTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGACCCTGGG CCCGGAGCTG 
GCGATCACGC TGCTGCTTGC TGTGCTCACG GAACACAAGC TGCTAGTCCA CTCGCTGCGG 
CCAGATCTGC TCACCAGCGT CTGCGAGGCC CTTGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG TGCCCCCGTG 
CCCTTCATCG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCACC TGTGGATGTC 
ATCTGTGTGG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACCC TGCCCCGCAG ACCCTACAAG GTTCTGCTGG CCACACTGAC ACATCTGTAC 
CAGCAGCTGG ATCAGACGTA TACTGGCCCC GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACGGACTATG AGGCAGTGTG TGGCCGCCGG GCCCGGCTAG AGCGCGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG CCTGCTCAAG GGCTACCGGG ACTTCCTGCG CCCACTCACC 
CAGGCCCCCT CTGAGGGGTC TCGCGATGTT GACAACCTCT TCTTCCTGCA AGGCTTCCTC 
AAGTCCCGAG AACGTTCCAG CCACAAGCTA TACTCCCAGC TGCTACACAC ACAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ACGCTGCCCT TGAATTCTTT 
GACTCTTGTG TCGACAAGGT CCACCCAGAG CAGGAGAAAC CTGAGCCCGT GCCCCTGGTG 
GAGCTGGAGG AGCTGTCGGG CAGTGAGCTC ACCGTGTTTA TCATGCCTCC CGAGGAGCCG 
CCAGCGCCCG AGGGCAGTGA ATCCACTCCC CAGTACTGTT ATGATGGGTT CCCTGAGCTA 
CGGGCTGAGC TGTTCGAGGC TCCTCAAGAA CAAGCCGGGG CTCTGCCTGT GCCAGGCCCT 
TCCCGCAGCG CCCCCAGCAG TCCTGCCCCT CGCCGCACCA AACAGGAGAT GAAGGTGGCG 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTGCTGGGC 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCCTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCAGACGGC CTACCAGGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCTGACG AGGTGTGTTA CCGGGTGCTG ATGCAGCTGT GCTCACACTA CGGGCAGCCC 
GTGCTGTCCG TGCGGGTCAT GCTGGACATG CGGCGGGCGG GCATCGTGCC CAACACCATC 
ACCTATGGCT ACTATAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG CACACCAGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTGGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGCGAG AACAGCGGCA GCGGCAGCAG AAGCGGGAGA AGGTGGAGAC AGCAGCCCAA 
GAGGCAGGCA GCTCGCAGAC AGAGCCCTAT TTGGAGCGCC CCTCCCCTAC CCGCCCGCTT 
CAGCGCCAGA CTACCTGGGC CGGGCGAAGC CTGCGGGACC CCGCCTCACC TATGGGGCGC 
CTGGTGAAGA GTGGAAGCCT GGGGAGTGCC CGAGGGGCAC AGCCCACCGT GGAGGCTGGT 
GTGGCCCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GGGGATCACC CATACCCTGG 
CATGATGGAA GCCTCTCAGA CCTGAGCCTG ACCGGGGAAG AGTCGGCACC CGGAGGCAGC 
CCAGGGGACT CAGGCTCAGC CCTGAGCACC CAGTCCACTG AAACCCTGGA AGGGCCAAGT 
GGGCGGGTGC CCAAGGCTGG TGGGCGTCAA GATGAGGCCA GCACCCCCCG AAGAGGGCTG 
GGCGCCCGCC TCCAACAGCT ACTCACTCCT TCCCGCCGCT CCCCCACCTC TCGTGCCCCC 
CCATCTGAGC TGCCCCCTGA CCTGCCTCCC TCAGCCCGTC GCAGCCCCAT GGACAGCCTT 
CTGCACCCCC GGGAGCGCCC TGGATCCACT GCATCCGAGA GCTCAGCCTC TCTGGGCAGT 
GAGTGGGACC TCTCAGAATC TTCTCTCAGC AGCGTGAGCC TTCGCCGTTC CTCAGAGCGC 
CTCAGTGACA CCCCTGGATC CTTCCAGCCA CCTTCCCTGG AAATTCTGCT GTCTAGCTGC 
TCGCTGTGCC GCGCCTGTGA CTCCCTGGTG TATGATGAGG AGATCATGGC TGGCTGGGCA 
CCTGACGACT CCAACCTCAA CACCACCTGT CCGTTCTGCG CCTGCCCCTT TGTGCCCCTG 
CTCAGCGTCC AGACCCTTGA TTCCCGACCC AGCGCCCCCA GCCCCAAGCC TGCTCCTGCT 
GGTGCCAGCG GCAGCAGCGA CGCTCCTGCC CCCGGGGGCC CGGGCCCTGT GCTCAGTGAC 
CGCAGGCTTT GCCTAGCCCT GGATGAGCCA CAGCTCTGCA ACGGGCACAT GGGGCGGCTG 
CGCCTGCCCA GTATTCTGCC GTGCTTGGTG CTGGCCTCCT GTGATGGGCC CCCGCCCCCC 
CAGGCCCCAG CTCCTTGGAT AATGCCTGAT CCAGCATCCG TGCAGGTGCG GCTGCTGTGG 
GATGTCCTGA CCCCTGATCC TGATAGCTGC CCACCTCTCT ATGTGCTCTG GAGGGTCCAC 
AGCCAGATCC CACAGCGGGT GGTATGGCCA GGTCCAGTAC CTGCATCCCT TAGCCTGGCG 
TTGCTGGAAT CGGTGCTGCG CCACGTTGGT CTCAACGAGG TGCACAAGGC TATAGGGCTC 
CTGCTAGAAA CTCTAGGGCC CCCTCCCACT GGCCTGCACC TACAAAGGGG CATCTACCGT 
GAGATCTTAT TCCTGACACT GGCTGCTCTG GGCAAGGACC ACATGGATAT AGTGGCCTTC 
GACAAGAAGT ACAAATCCGC CTTTAACAAG CTGGCCAGCA GCATGGGCAA GGAGGAGCTG 
AGGCAGCGGC GGGCACAGAT GCCTACCCCA AAGGCCATTG ATTGCCGGAA ATGTTTCGGA 
GCGCCTCTGG AATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044205.1
CDS	659..4996
Translation	MEADAVSEGGAMAEERPPRLVDYFVIAGLAGNGAPIPEETWIPEPSGPLRPPRPAEPITDVAVIAKALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQTLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPVDVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTHLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFFLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPVPLVELEELSGSELTVFIMPPEEPPAPEGSESTPQYCYDGFPELRAELFEAPQEQAGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALQTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLDMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLREQRQRQQKREKVETAAQEAGSSQTEPYLERPSPTRPLQRQTTWAGRSLRDPASPMGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPIPWHDGSLSDLSLTGEESAPGGSPGDSGSALSTQSTETLEGPSGRVPKAGGRQDEASTPRRGLGARLQQLLTPSRRSPTSRAPPSELPPDLPPSARRSPMDSLLHPRERPGSTASESSASLGSEWDLSESSLSSVSLRRSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKPAPAGASGSSDAPAPGGPGPVLSDRRLCLALDEPQLCNGHMGRLRLPSILPCLVLASCDGPPPPQAPAPWIMPDPASVQVRLLWDVLTPDPDSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVHKAIGLLLETLGPPPTGLHLQRGIYREILFLTLAALGKDHMDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_006044143.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis DENN domain containing 4B (DENND4B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044143.2

ORF Insert Sequence:

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TCGTGATAGC TGGACTTGCA GGGAACGGAG CACCCATCCC TGAGGAAACA 
TGGATTCCTG AACCCAGTGG ACCCCTGCGC CCTCCTCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAAGGC CCTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCCGCGG GGGGCCACCC CTTGGAACTC AGCGCTGGGC TCCTAGGTGG AACTCAACCT 
GTCATCTGCT ACCGGAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ATACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGCG AGGGCACTCC TCATACTTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCCGTGCC CGTCTTCTCC ACCTTTGTGC TCACGGGTGC AGCTGGTGAT 
AAGGTGTACG GTGCTGCCCT GCAGTTCTAC GAGGCGTTCC CGAGGGCCAG GCTGTCGGAG 
CGGCAGGCTC GGGCCCTGGG CCTGCTGAGT GCTGTGGAGC GGGGCCGGGC ACTGGGGGGC 
CGGGCTGTGC GCAGCCGCCG CGCCATCGCT GTGCTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGCGCCT TCCTCACCTT CCTCTACCGC TACTCCGTCT CGGGCCCCCA CCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AATGTTCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGCCAGCC TGTATCCTCA 
CCCCTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGACCCTGGG CCCGGAGCTG 
GCGATCACGC TGCTGCTTGC TGTGCTCACG GAACACAAGC TGCTAGTCCA CTCGCTGCGG 
CCAGATCTGC TCACCAGCGT CTGCGAGGCC CTTGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG TGCCCCCGTG 
CCCTTCATCG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCACC TGTGGATGTC 
ATCTGTGTGG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACCC TGCCCCGCAG ACCCTACAAG GTTCTGCTGG CCACACTGAC ACATCTGTAC 
CAGCAGCTGG ATCAGACGTA TACTGGCCCC GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACGGACTATG AGGCAGTGTG TGGCCGCCGG GCCCGGCTAG AGCGCGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG CCTGCTCAAG GGCTACCGGG ACTTCCTGCG CCCACTCACC 
CAGGCCCCCT CTGAGGGGTC TCGCGATGTT GACAACCTCT TCTTCCTGCA AGGCTTCCTC 
AAGTCCCGAG AACGTTCCAG CCACAAGCTA TACTCCCAGC TGCTACACAC ACAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ACGCTGCCCT TGAATTCTTT 
GACTCTTGTG TCGACAAGGT CCACCCAGAG CAGGAGAAAC CTGAGCCCGT GCCCCTGGTG 
GAGCTGGAGG AGCTGTCGGG CAGTGAGCTC ACCGTGTTTA TCATGCCTCC CGAGGAGCCG 
CCAGCGCCCG AGGGCAGTGA ATCCACTCCC CAGTACTGTT ATGATGGGTT CCCTGAGCTA 
CGGGCTGAGC TGTTCGAGGC TCCTCAAGAA CAAGCCGGGG CTCTGCCTGT GCCAGGCCCT 
TCCCGCAGCG CCCCCAGCAG TCCTGCCCCT CGCCGCACCA AACAGGAGAT GAAGGTGGCG 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTGCTGGGC 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCCTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCAGACGGC CTACCAGGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCTGACG AGGTGTGTTA CCGGGTGCTG ATGCAGCTGT GCTCACACTA CGGGCAGCCC 
GTGCTGTCCG TGCGGGTCAT GCTGGACATG CGGCGGGCGG GCATCGTGCC CAACACCATC 
ACCTATGGCT ACTATAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG CACACCAGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTGGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGCGAG AACAGCGGCA GCGGCAGCAG AAGCGGGAGA AGGTGGAGAC AGCAGCCCAA 
GAGGCAGGCA GCTCGCAGAC AGAGCCCTAT TTGGAGCGCC CCTCCCCTAC CCGCCCGCTT 
CAGCGCCAGA CTACCTGGGC CGGGCGAAGC CTGCGGGACC CCGCCTCACC TATGGGGCGC 
CTGGTGAAGA GTGGAAGCCT GGGGAGTGCC CGAGGGGCAC AGCCCACCGT GGAGGCTGGT 
GTGGCCCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GGGGATCACC CATACCCTGG 
CATGATGGAA GCCTCTCAGA CCTGAGCCTG ACCGGGGAAG AGTCGGCACC CGGAGGCAGC 
CCAGGGGACT CAGGCTCAGC CCTGAGCACC CAGTCCACTG AAACCCTGGA AGGGCCAAGT 
GGGCGGGTGC CCAAGGCTGG TGGGCGTCAA GATGAGGCCA GCACCCCCCG AAGAGGGCTG 
GGCGCCCGCC TCCAACAGCT ACTCACTCCT TCCCGCCGCT CCCCCACCTC TCGTGCCCCC 
CCATCTGAGC TGCCCCCTGA CCTGCCTCCC TCAGCCCGTC GCAGCCCCAT GGACAGCCTT 
CTGCACCCCC GGGAGCGCCC TGGATCCACT GCATCCGAGA GCTCAGCCTC TCTGGGCAGT 
GAGTGGGACC TCTCAGAATC TTCTCTCAGC AGCGTGAGCC TTCGCCGTTC CTCAGAGCGC 
CTCAGTGACA CCCCTGGATC CTTCCAGCCA CCTTCCCTGG AAATTCTGCT GTCTAGCTGC 
TCGCTGTGCC GCGCCTGTGA CTCCCTGGTG TATGATGAGG AGATCATGGC TGGCTGGGCA 
CCTGACGACT CCAACCTCAA CACCACCTGT CCGTTCTGCG CCTGCCCCTT TGTGCCCCTG 
CTCAGCGTCC AGACCCTTGA TTCCCGACCC AGCGCCCCCA GCCCCAAGCC TGCTCCTGCT 
GGTGCCAGCG GCAGCAGCGA CGCTCCTGCC CCCGGGGGCC CGGGCCCTGT GCTCAGTGAC 
CGCAGGCTTT GCCTAGCCCT GGATGAGCCA CAGCTCTGCA ACGGGCACAT GGGGCGGCTG 
CGCCTGCCCA GTATTCTGCC GTGCTTGGTG CTGGCCTCCT GTGATGGGCC CCCGCCCCCC 
CAGGCCCCAG CTCCTTGGAT AATGCCTGAT CCAGCATCCG TGCAGGTGCG GCTGCTGTGG 
GATGTCCTGA CCCCTGATCC TGATAGCTGC CCACCTCTCT ATGTGCTCTG GAGGGTCCAC 
AGCCAGATCC CACAGCGGGT GGTATGGCCA GGTCCAGTAC CTGCATCCCT TAGCCTGGCG 
TTGCTGGAAT CGGTGCTGCG CCACGTTGGT CTCAACGAGG TGCACAAGGC TATAGGGCTC 
CTGCTAGAAA CTCTAGGGCC CCCTCCCACT GGCCTGCACC TACAAAGGGG CATCTACCGT 
GAGATCTTAT TCCTGACACT GGCTGCTCTG GGCAAGGACC ACATGGATAT AGTGGCCTTC 
GACAAGAAGT ACAAATCCGC CTTTAACAAG CTGGCCAGCA GCATGGGCAA GGAGGAGCTG 
AGGCAGCGGC GGGCACAGAT GCCTACCCCA AAGGCCATTG ATTGCCGGAA ATGTTTCGGA 
GCGCCTCTGG AATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4B ( XM_006044143.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044205.1 Bubalus bubalis DENN/MADD domain containing 4B (DENND4B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu03360
Clone ID Related Accession (Same CDS sequence)	XM_006044143.2 , XM_006044143.1
Accession Version	XM_006044143.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	DENN domain-containing protein 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783544.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TCGTGATAGC TGGACTTGCA GGGAACGGAG CACCCATCCC TGAGGAAACA 
TGGATTCCTG AACCCAGTGG ACCCCTGCGC CCTCCTCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAAGGC CCTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCCGCGG GGGGCCACCC CTTGGAACTC AGCGCTGGGC TCCTAGGTGG AACTCAACCT 
GTCATCTGCT ACCGGAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ATACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGCG AGGGCACTCC TCATACTTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCCGTGCC CGTCTTCTCC ACCTTTGTGC TCACGGGTGC AGCTGGTGAT 
AAGGTGTACG GTGCTGCCCT GCAGTTCTAC GAGGCGTTCC CGAGGGCCAG GCTGTCGGAG 
CGGCAGGCTC GGGCCCTGGG CCTGCTGAGT GCTGTGGAGC GGGGCCGGGC ACTGGGGGGC 
CGGGCTGTGC GCAGCCGCCG CGCCATCGCT GTGCTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGCGCCT TCCTCACCTT CCTCTACCGC TACTCCGTCT CGGGCCCCCA CCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AATGTTCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGCCAGCC TGTATCCTCA 
CCCCTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGACCCTGGG CCCGGAGCTG 
GCGATCACGC TGCTGCTTGC TGTGCTCACG GAACACAAGC TGCTAGTCCA CTCGCTGCGG 
CCAGATCTGC TCACCAGCGT CTGCGAGGCC CTTGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG TGCCCCCGTG 
CCCTTCATCG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCACC TGTGGATGTC 
ATCTGTGTGG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACCC TGCCCCGCAG ACCCTACAAG GTTCTGCTGG CCACACTGAC ACATCTGTAC 
CAGCAGCTGG ATCAGACGTA TACTGGCCCC GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACGGACTATG AGGCAGTGTG TGGCCGCCGG GCCCGGCTAG AGCGCGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG CCTGCTCAAG GGCTACCGGG ACTTCCTGCG CCCACTCACC 
CAGGCCCCCT CTGAGGGGTC TCGCGATGTT GACAACCTCT TCTTCCTGCA AGGCTTCCTC 
AAGTCCCGAG AACGTTCCAG CCACAAGCTA TACTCCCAGC TGCTACACAC ACAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ACGCTGCCCT TGAATTCTTT 
GACTCTTGTG TCGACAAGGT CCACCCAGAG CAGGAGAAAC CTGAGCCCGT GCCCCTGGTG 
GAGCTGGAGG AGCTGTCGGG CAGTGAGCTC ACCGTGTTTA TCATGCCTCC CGAGGAGCCG 
CCAGCGCCCG AGGGCAGTGA ATCCACTCCC CAGTACTGTT ATGATGGGTT CCCTGAGCTA 
CGGGCTGAGC TGTTCGAGGC TCCTCAAGAA CAAGCCGGGG CTCTGCCTGT GCCAGGCCCT 
TCCCGCAGCG CCCCCAGCAG TCCTGCCCCT CGCCGCACCA AACAGGAGAT GAAGGTGGCG 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTGCTGGGC 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCCTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCAGACGGC CTACCAGGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCTGACG AGGTGTGTTA CCGGGTGCTG ATGCAGCTGT GCTCACACTA CGGGCAGCCC 
GTGCTGTCCG TGCGGGTCAT GCTGGACATG CGGCGGGCGG GCATCGTGCC CAACACCATC 
ACCTATGGCT ACTATAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG CACACCAGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTGGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGCGAG AACAGCGGCA GCGGCAGCAG AAGCGGGAGA AGGTGGAGAC AGCAGCCCAA 
GAGGCAGGCA GCTCGCAGAC AGAGCCCTAT TTGGAGCGCC CCTCCCCTAC CCGCCCGCTT 
CAGCGCCAGA CTACCTGGGC CGGGCGAAGC CTGCGGGACC CCGCCTCACC TATGGGGCGC 
CTGGTGAAGA GTGGAAGCCT GGGGAGTGCC CGAGGGGCAC AGCCCACCGT GGAGGCTGGT 
GTGGCCCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GGGGATCACC CATACCCTGG 
CATGATGGAA GCCTCTCAGA CCTGAGCCTG ACCGGGGAAG AGTCGGCACC CGGAGGCAGC 
CCAGGGGACT CAGGCTCAGC CCTGAGCACC CAGTCCACTG AAACCCTGGA AGGGCCAAGT 
GGGCGGGTGC CCAAGGCTGG TGGGCGTCAA GATGAGGCCA GCACCCCCCG AAGAGGGCTG 
GGCGCCCGCC TCCAACAGCT ACTCACTCCT TCCCGCCGCT CCCCCACCTC TCGTGCCCCC 
CCATCTGAGC TGCCCCCTGA CCTGCCTCCC TCAGCCCGTC GCAGCCCCAT GGACAGCCTT 
CTGCACCCCC GGGAGCGCCC TGGATCCACT GCATCCGAGA GCTCAGCCTC TCTGGGCAGT 
GAGTGGGACC TCTCAGAATC TTCTCTCAGC AGCGTGAGCC TTCGCCGTTC CTCAGAGCGC 
CTCAGTGACA CCCCTGGATC CTTCCAGCCA CCTTCCCTGG AAATTCTGCT GTCTAGCTGC 
TCGCTGTGCC GCGCCTGTGA CTCCCTGGTG TATGATGAGG AGATCATGGC TGGCTGGGCA 
CCTGACGACT CCAACCTCAA CACCACCTGT CCGTTCTGCG CCTGCCCCTT TGTGCCCCTG 
CTCAGCGTCC AGACCCTTGA TTCCCGACCC AGCGCCCCCA GCCCCAAGCC TGCTCCTGCT 
GGTGCCAGCG GCAGCAGCGA CGCTCCTGCC CCCGGGGGCC CGGGCCCTGT GCTCAGTGAC 
CGCAGGCTTT GCCTAGCCCT GGATGAGCCA CAGCTCTGCA ACGGGCACAT GGGGCGGCTG 
CGCCTGCCCA GTATTCTGCC GTGCTTGGTG CTGGCCTCCT GTGATGGGCC CCCGCCCCCC 
CAGGCCCCAG CTCCTTGGAT AATGCCTGAT CCAGCATCCG TGCAGGTGCG GCTGCTGTGG 
GATGTCCTGA CCCCTGATCC TGATAGCTGC CCACCTCTCT ATGTGCTCTG GAGGGTCCAC 
AGCCAGATCC CACAGCGGGT GGTATGGCCA GGTCCAGTAC CTGCATCCCT TAGCCTGGCG 
TTGCTGGAAT CGGTGCTGCG CCACGTTGGT CTCAACGAGG TGCACAAGGC TATAGGGCTC 
CTGCTAGAAA CTCTAGGGCC CCCTCCCACT GGCCTGCACC TACAAAGGGG CATCTACCGT 
GAGATCTTAT TCCTGACACT GGCTGCTCTG GGCAAGGACC ACATGGATAT AGTGGCCTTC 
GACAAGAAGT ACAAATCCGC CTTTAACAAG CTGGCCAGCA GCATGGGCAA GGAGGAGCTG 
AGGCAGCGGC GGGCACAGAT GCCTACCCCA AAGGCCATTG ATTGCCGGAA ATGTTTCGGA 
GCGCCTCTGG AATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044205.1
CDS	1032..5369
Translation	MEADAVSEGGAMAEERPPRLVDYFVIAGLAGNGAPIPEETWIPEPSGPLRPPRPAEPITDVAVIAKALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQTLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPVDVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTHLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFFLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPVPLVELEELSGSELTVFIMPPEEPPAPEGSESTPQYCYDGFPELRAELFEAPQEQAGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALQTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLDMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLREQRQRQQKREKVETAAQEAGSSQTEPYLERPSPTRPLQRQTTWAGRSLRDPASPMGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPIPWHDGSLSDLSLTGEESAPGGSPGDSGSALSTQSTETLEGPSGRVPKAGGRQDEASTPRRGLGARLQQLLTPSRRSPTSRAPPSELPPDLPPSARRSPMDSLLHPRERPGSTASESSASLGSEWDLSESSLSSVSLRRSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKPAPAGASGSSDAPAPGGPGPVLSDRRLCLALDEPQLCNGHMGRLRLPSILPCLVLASCDGPPPPQAPAPWIMPDPASVQVRLLWDVLTPDPDSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVHKAIGLLLETLGPPPTGLHLQRGIYREILFLTLAALGKDHMDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_006044143.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis DENN/MADD domain containing 4B (DENND4B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044143.1

ORF Insert Sequence:

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TCGTGATAGC TGGACTTGCA GGGAACGGAG CACCCATCCC TGAGGAAACA 
TGGATTCCTG AACCCAGTGG ACCCCTGCGC CCTCCTCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAAGGC CCTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCCGCGG GGGGCCACCC CTTGGAACTC AGCGCTGGGC TCCTAGGTGG AACTCAACCT 
GTCATCTGCT ACCGGAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ATACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGCG AGGGCACTCC TCATACTTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCCGTGCC CGTCTTCTCC ACCTTTGTGC TCACGGGTGC AGCTGGTGAT 
AAGGTGTACG GTGCTGCCCT GCAGTTCTAC GAGGCGTTCC CGAGGGCCAG GCTGTCGGAG 
CGGCAGGCTC GGGCCCTGGG CCTGCTGAGT GCTGTGGAGC GGGGCCGGGC ACTGGGGGGC 
CGGGCTGTGC GCAGCCGCCG CGCCATCGCT GTGCTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGCGCCT TCCTCACCTT CCTCTACCGC TACTCCGTCT CGGGCCCCCA CCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AATGTTCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGCCAGCC TGTATCCTCA 
CCCCTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGACCCTGGG CCCGGAGCTG 
GCGATCACGC TGCTGCTTGC TGTGCTCACG GAACACAAGC TGCTAGTCCA CTCGCTGCGG 
CCAGATCTGC TCACCAGCGT CTGCGAGGCC CTTGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG TGCCCCCGTG 
CCCTTCATCG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCACC TGTGGATGTC 
ATCTGTGTGG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACCC TGCCCCGCAG ACCCTACAAG GTTCTGCTGG CCACACTGAC ACATCTGTAC 
CAGCAGCTGG ATCAGACGTA TACTGGCCCC GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACGGACTATG AGGCAGTGTG TGGCCGCCGG GCCCGGCTAG AGCGCGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG CCTGCTCAAG GGCTACCGGG ACTTCCTGCG CCCACTCACC 
CAGGCCCCCT CTGAGGGGTC TCGCGATGTT GACAACCTCT TCTTCCTGCA AGGCTTCCTC 
AAGTCCCGAG AACGTTCCAG CCACAAGCTA TACTCCCAGC TGCTACACAC ACAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ACGCTGCCCT TGAATTCTTT 
GACTCTTGTG TCGACAAGGT CCACCCAGAG CAGGAGAAAC CTGAGCCCGT GCCCCTGGTG 
GAGCTGGAGG AGCTGTCGGG CAGTGAGCTC ACCGTGTTTA TCATGCCTCC CGAGGAGCCG 
CCAGCGCCCG AGGGCAGTGA ATCCACTCCC CAGTACTGTT ATGATGGGTT CCCTGAGCTA 
CGGGCTGAGC TGTTCGAGGC TCCTCAAGAA CAAGCCGGGG CTCTGCCTGT GCCAGGCCCT 
TCCCGCAGCG CCCCCAGCAG TCCTGCCCCT CGCCGCACCA AACAGGAGAT GAAGGTGGCG 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTGCTGGGC 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCCTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCAGACGGC CTACCAGGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCTGACG AGGTGTGTTA CCGGGTGCTG ATGCAGCTGT GCTCACACTA CGGGCAGCCC 
GTGCTGTCCG TGCGGGTCAT GCTGGACATG CGGCGGGCGG GCATCGTGCC CAACACCATC 
ACCTATGGCT ACTATAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG CACACCAGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTGGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGCGAG AACAGCGGCA GCGGCAGCAG AAGCGGGAGA AGGTGGAGAC AGCAGCCCAA 
GAGGCAGGCA GCTCGCAGAC AGAGCCCTAT TTGGAGCGCC CCTCCCCTAC CCGCCCGCTT 
CAGCGCCAGA CTACCTGGGC CGGGCGAAGC CTGCGGGACC CCGCCTCACC TATGGGGCGC 
CTGGTGAAGA GTGGAAGCCT GGGGAGTGCC CGAGGGGCAC AGCCCACCGT GGAGGCTGGT 
GTGGCCCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GGGGATCACC CATACCCTGG 
CATGATGGAA GCCTCTCAGA CCTGAGCCTG ACCGGGGAAG AGTCGGCACC CGGAGGCAGC 
CCAGGGGACT CAGGCTCAGC CCTGAGCACC CAGTCCACTG AAACCCTGGA AGGGCCAAGT 
GGGCGGGTGC CCAAGGCTGG TGGGCGTCAA GATGAGGCCA GCACCCCCCG AAGAGGGCTG 
GGCGCCCGCC TCCAACAGCT ACTCACTCCT TCCCGCCGCT CCCCCACCTC TCGTGCCCCC 
CCATCTGAGC TGCCCCCTGA CCTGCCTCCC TCAGCCCGTC GCAGCCCCAT GGACAGCCTT 
CTGCACCCCC GGGAGCGCCC TGGATCCACT GCATCCGAGA GCTCAGCCTC TCTGGGCAGT 
GAGTGGGACC TCTCAGAATC TTCTCTCAGC AGCGTGAGCC TTCGCCGTTC CTCAGAGCGC 
CTCAGTGACA CCCCTGGATC CTTCCAGCCA CCTTCCCTGG AAATTCTGCT GTCTAGCTGC 
TCGCTGTGCC GCGCCTGTGA CTCCCTGGTG TATGATGAGG AGATCATGGC TGGCTGGGCA 
CCTGACGACT CCAACCTCAA CACCACCTGT CCGTTCTGCG CCTGCCCCTT TGTGCCCCTG 
CTCAGCGTCC AGACCCTTGA TTCCCGACCC AGCGCCCCCA GCCCCAAGCC TGCTCCTGCT 
GGTGCCAGCG GCAGCAGCGA CGCTCCTGCC CCCGGGGGCC CGGGCCCTGT GCTCAGTGAC 
CGCAGGCTTT GCCTAGCCCT GGATGAGCCA CAGCTCTGCA ACGGGCACAT GGGGCGGCTG 
CGCCTGCCCA GTATTCTGCC GTGCTTGGTG CTGGCCTCCT GTGATGGGCC CCCGCCCCCC 
CAGGCCCCAG CTCCTTGGAT AATGCCTGAT CCAGCATCCG TGCAGGTGCG GCTGCTGTGG 
GATGTCCTGA CCCCTGATCC TGATAGCTGC CCACCTCTCT ATGTGCTCTG GAGGGTCCAC 
AGCCAGATCC CACAGCGGGT GGTATGGCCA GGTCCAGTAC CTGCATCCCT TAGCCTGGCG 
TTGCTGGAAT CGGTGCTGCG CCACGTTGGT CTCAACGAGG TGCACAAGGC TATAGGGCTC 
CTGCTAGAAA CTCTAGGGCC CCCTCCCACT GGCCTGCACC TACAAAGGGG CATCTACCGT 
GAGATCTTAT TCCTGACACT GGCTGCTCTG GGCAAGGACC ACATGGATAT AGTGGCCTTC 
GACAAGAAGT ACAAATCCGC CTTTAACAAG CTGGCCAGCA GCATGGGCAA GGAGGAGCTG 
AGGCAGCGGC GGGCACAGAT GCCTACCCCA AAGGCCATTG ATTGCCGGAA ATGTTTCGGA 
GCGCCTCTGG AATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4B ( XM_006044144.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044206.1 Bubalus bubalis DENN domain containing 4B (DENND4B), transcript variant X5, mRNA.

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CloneID	OBu03361
Clone ID Related Accession (Same CDS sequence)	XM_006044144.2 , XM_006044144.1
Accession Version	XM_006044144.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	DENN domain-containing protein 4B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006044144.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TCGTGATAGC TGGACTTGCA GGGAACGGAG CACCCATCCC TGAGGAAACA 
TGGATTCCTG AACCCAGTGG ACCCCTGCGC CCTCCTCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAAGGC CCTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCCGCGG GGGGCCACCC CTTGGAACTC AGCGCTGGGC TCCTAGGTGG AACTCAACCT 
GTCATCTGCT ACCGGAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ATACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGCG AGGGCACTCC TCATACTTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCCGTGCC CGTCTTCTCC ACCTTTGTGC TCACGGGTGC AGCTGGTGAT 
AAGGTGTACG GTGCTGCCCT GCAGTTCTAC GAGGCGTTCC CGAGGGCCAG GCTGTCGGAG 
CGGCAGGCTC GGGCCCTGGG CCTGCTGAGT GCTGTGGAGC GGGGCCGGGC ACTGGGGGGC 
CGGGCTGTGC GCAGCCGCCG CGCCATCGCT GTGCTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGCGCCT TCCTCACCTT CCTCTACCGC TACTCCGTCT CGGGCCCCCA CCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AATGTTCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGCCAGCC TGTATCCTCA 
CCCCTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGACCCTGGG CCCGGAGCTG 
GCGATCACGC TGCTGCTTGC TGTGCTCACG GAACACAAGC TGCTAGTCCA CTCGCTGCGG 
CCAGATCTGC TCACCAGCGT CTGCGAGGCC CTTGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG TGCCCCCGTG 
CCCTTCATCG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCACC TGTGGATGTC 
ATCTGTGTGG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACCC TGCCCCGCAG ACCCTACAAG GTTCTGCTGG CCACACTGAC ACATCTGTAC 
CAGCAGCTGG ATCAGACGTA TACTGGCCCC GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACGGACTATG AGGCAGTGTG TGGCCGCCGG GCCCGGCTAG AGCGCGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG CCTGCTCAAG GGCTACCGGG ACTTCCTGCG CCCACTCACC 
CAGGCCCCCT CTGAGGGGTC TCGCGATGTT GACAACCTCT TCTTCCTGCA AGGCTTCCTC 
AAGTCCCGAG AACGTTCCAG CCACAAGCTA TACTCCCAGC TGCTACACAC ACAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ACGCTGCCCT TGAATTCTTT 
GACTCTTGTG TCGACAAGGT CCACCCAGAG CAGGAGAAAC CTGAGCCCGT GCCCCTGGTG 
GAGCTGGAGG AGCTGTCGGG CAGTGAGCTC ACCGTGTTTA TCATGCCTCC CGAGGAGCCG 
CCAGCGCCCG AGGGCAGTGA ATCCACTCCC CAGTACTGTT ATGATGGGTT CCCTGAGCTA 
CGGGCTGAGC TGTTCGAGGC TCCTCAAGAA CAAGCCGGGG CTCTGCCTGT GCCAGGCCCT 
TCCCGCAGCG CCCCCAGCAG TCCTGCCCCT CGCCGCACCA AACAGGAGAT GAAGGTGGCG 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTGCTGGGC 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCCTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCAGACGGC CTACCAGGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCTGACG AGGTGTGTTA CCGGGTGCTG ATGCAGCTGT GCTCACACTA CGGGCAGCCC 
GTGCTGTCCG TGCGGGTCAT GCTGGACATG CGGCGGGCGG GCATCGTGCC CAACACCATC 
ACCTATGGCT ACTATAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG CACACCAGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTGGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGCGAG AACAGCGGCA GCGGCAGCAG AAGCGGGAGA AGGTGGAGAC AGCAGCCCAA 
GAGGCAGGCA GCTCGCAGAC AGAGCCCTAT TTGGAGCGCC CCTCCCCTAC CCGCCCGCTT 
CAGCGCCAGA CTACCTGGGC CGGGCGAAGC CTGCGGGACC CCGCCTCACC TATGGGGCGC 
CTGGTGAAGA GTGGAAGCCT GGGGAGTGCC CGAGGGGCAC AGCCCACCGT GGAGGCTGGT 
GTGGCCCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GGGGATCACC CATACCCTGG 
CATGATGGAA GCCTCTCAGA CCTGAGCCTG ACCGGGGAAG AGTCGGCACC CGGAGGCAGC 
CCAGGGGACT CAGGCTCAGC CCTGAGCACC CAGTCCACTG AAACCCTGGA AGGGCCAAGT 
GGGCGGGTGC CCAAGGCTGG TGGGCGTCAA GATGAGGCCA GCACCCCCCG AAGAGGGCTG 
GGCGCCCGCC TCCAACAGCT ACTCACTCCT TCCCGCCGCT CCCCCACCTC TCGTGCCCCC 
CCATCTGAGC TGCCCCCTGA CCTGCCTCCC TCAGCCCGTC GCAGCCCCAT GGACAGCCTT 
CTGCACCCCC GGGAGCGCCC TGGATCCACT GCATCCGAGA GCTCAGCCTC TCTGGGCAGT 
GAGTGGGACC TCTCAGAATC TTCTCTCAGC AGCGTGAGCC TTCGCCGTTC CTCAGAGCGC 
CTCAGTGACA CCCCTGGATC CTTCCAGCCA CCTTCCCTGG AAATTCTGCT GTCTAGCTGC 
TCGCTGTGCC GCGCCTGTGA CTCCCTGGTG TATGATGAGG AGATCATGGC TGGCTGGGCA 
CCTGACGACT CCAACCTCAA CACCACCTGT CCGTTCTGCG CCTGCCCCTT TGTGCCCCTG 
CTCAGCGTCC AGACCCTTGA TTCCCGACCC AGCGCCCCCA GCCCCAAGCC TGCTCCTGCT 
GGTGCCAGCG GCAGCAGCGA CGCTCCTGCC CCCGGGGGCC CGGGCCCTGT GCTCAGTGAC 
CGCAGGCTTT GCCTAGCCCT GGATGAGCCA CAGCTCTGCA ACGGGCACAT GGGGACTGCC 
TCCCGGCGTG TTGAGAGTGG GGCATGGGCG TATCTCAGCC CCCTCGTGCT GCGGAAGGAG 
CTGGAGTCAC TGGTAGAGAA CGAGGGCAGT GAGGTGCTGG CATTGCCTGA GTTGCCTGCC 
GCTCACCCCA TCATCTTCTG GAACCTTCTG TGGTATTTCC AGCGGCTGCG CCTGCCCAGT 
ATTCTGCCGT GCTTGGTGCT GGCCTCCTGT GATGGGCCCC CGCCCCCCCA GGCCCCAGCT 
CCTTGGATAA TGCCTGATCC AGCATCCGTG CAGGTGCGGC TGCTGTGGGA TGTCCTGACC 
CCTGATCCTG ATAGCTGCCC ACCTCTCTAT GTGCTCTGGA GGGTCCACAG GGCATCTACC 
GTGAGATCTT ATTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044206.1
CDS	659..4816
Translation	MEADAVSEGGAMAEERPPRLVDYFVIAGLAGNGAPIPEETWIPEPSGPLRPPRPAEPITDVAVIAKALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQTLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPVDVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTHLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFFLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPVPLVELEELSGSELTVFIMPPEEPPAPEGSESTPQYCYDGFPELRAELFEAPQEQAGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALQTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLDMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLREQRQRQQKREKVETAAQEAGSSQTEPYLERPSPTRPLQRQTTWAGRSLRDPASPMGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPIPWHDGSLSDLSLTGEESAPGGSPGDSGSALSTQSTETLEGPSGRVPKAGGRQDEASTPRRGLGARLQQLLTPSRRSPTSRAPPSELPPDLPPSARRSPMDSLLHPRERPGSTASESSASLGSEWDLSESSLSSVSLRRSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKPAPAGASGSSDAPAPGGPGPVLSDRRLCLALDEPQLCNGHMGTASRRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPCLVLASCDGPPPPQAPAPWIMPDPASVQVRLLWDVLTPDPDSCPPLYVLWRVHRASTVRSYS

Target ORF information:

RefSeq Version	XM_006044144.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis DENN domain containing 4B (DENND4B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044144.2

ORF Insert Sequence:

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TCGTGATAGC TGGACTTGCA GGGAACGGAG CACCCATCCC TGAGGAAACA 
TGGATTCCTG AACCCAGTGG ACCCCTGCGC CCTCCTCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAAGGC CCTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCCGCGG GGGGCCACCC CTTGGAACTC AGCGCTGGGC TCCTAGGTGG AACTCAACCT 
GTCATCTGCT ACCGGAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ATACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGCG AGGGCACTCC TCATACTTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCCGTGCC CGTCTTCTCC ACCTTTGTGC TCACGGGTGC AGCTGGTGAT 
AAGGTGTACG GTGCTGCCCT GCAGTTCTAC GAGGCGTTCC CGAGGGCCAG GCTGTCGGAG 
CGGCAGGCTC GGGCCCTGGG CCTGCTGAGT GCTGTGGAGC GGGGCCGGGC ACTGGGGGGC 
CGGGCTGTGC GCAGCCGCCG CGCCATCGCT GTGCTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGCGCCT TCCTCACCTT CCTCTACCGC TACTCCGTCT CGGGCCCCCA CCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AATGTTCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGCCAGCC TGTATCCTCA 
CCCCTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGACCCTGGG CCCGGAGCTG 
GCGATCACGC TGCTGCTTGC TGTGCTCACG GAACACAAGC TGCTAGTCCA CTCGCTGCGG 
CCAGATCTGC TCACCAGCGT CTGCGAGGCC CTTGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG TGCCCCCGTG 
CCCTTCATCG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCACC TGTGGATGTC 
ATCTGTGTGG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACCC TGCCCCGCAG ACCCTACAAG GTTCTGCTGG CCACACTGAC ACATCTGTAC 
CAGCAGCTGG ATCAGACGTA TACTGGCCCC GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACGGACTATG AGGCAGTGTG TGGCCGCCGG GCCCGGCTAG AGCGCGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG CCTGCTCAAG GGCTACCGGG ACTTCCTGCG CCCACTCACC 
CAGGCCCCCT CTGAGGGGTC TCGCGATGTT GACAACCTCT TCTTCCTGCA AGGCTTCCTC 
AAGTCCCGAG AACGTTCCAG CCACAAGCTA TACTCCCAGC TGCTACACAC ACAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ACGCTGCCCT TGAATTCTTT 
GACTCTTGTG TCGACAAGGT CCACCCAGAG CAGGAGAAAC CTGAGCCCGT GCCCCTGGTG 
GAGCTGGAGG AGCTGTCGGG CAGTGAGCTC ACCGTGTTTA TCATGCCTCC CGAGGAGCCG 
CCAGCGCCCG AGGGCAGTGA ATCCACTCCC CAGTACTGTT ATGATGGGTT CCCTGAGCTA 
CGGGCTGAGC TGTTCGAGGC TCCTCAAGAA CAAGCCGGGG CTCTGCCTGT GCCAGGCCCT 
TCCCGCAGCG CCCCCAGCAG TCCTGCCCCT CGCCGCACCA AACAGGAGAT GAAGGTGGCG 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTGCTGGGC 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCCTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCAGACGGC CTACCAGGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCTGACG AGGTGTGTTA CCGGGTGCTG ATGCAGCTGT GCTCACACTA CGGGCAGCCC 
GTGCTGTCCG TGCGGGTCAT GCTGGACATG CGGCGGGCGG GCATCGTGCC CAACACCATC 
ACCTATGGCT ACTATAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG CACACCAGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTGGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGCGAG AACAGCGGCA GCGGCAGCAG AAGCGGGAGA AGGTGGAGAC AGCAGCCCAA 
GAGGCAGGCA GCTCGCAGAC AGAGCCCTAT TTGGAGCGCC CCTCCCCTAC CCGCCCGCTT 
CAGCGCCAGA CTACCTGGGC CGGGCGAAGC CTGCGGGACC CCGCCTCACC TATGGGGCGC 
CTGGTGAAGA GTGGAAGCCT GGGGAGTGCC CGAGGGGCAC AGCCCACCGT GGAGGCTGGT 
GTGGCCCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GGGGATCACC CATACCCTGG 
CATGATGGAA GCCTCTCAGA CCTGAGCCTG ACCGGGGAAG AGTCGGCACC CGGAGGCAGC 
CCAGGGGACT CAGGCTCAGC CCTGAGCACC CAGTCCACTG AAACCCTGGA AGGGCCAAGT 
GGGCGGGTGC CCAAGGCTGG TGGGCGTCAA GATGAGGCCA GCACCCCCCG AAGAGGGCTG 
GGCGCCCGCC TCCAACAGCT ACTCACTCCT TCCCGCCGCT CCCCCACCTC TCGTGCCCCC 
CCATCTGAGC TGCCCCCTGA CCTGCCTCCC TCAGCCCGTC GCAGCCCCAT GGACAGCCTT 
CTGCACCCCC GGGAGCGCCC TGGATCCACT GCATCCGAGA GCTCAGCCTC TCTGGGCAGT 
GAGTGGGACC TCTCAGAATC TTCTCTCAGC AGCGTGAGCC TTCGCCGTTC CTCAGAGCGC 
CTCAGTGACA CCCCTGGATC CTTCCAGCCA CCTTCCCTGG AAATTCTGCT GTCTAGCTGC 
TCGCTGTGCC GCGCCTGTGA CTCCCTGGTG TATGATGAGG AGATCATGGC TGGCTGGGCA 
CCTGACGACT CCAACCTCAA CACCACCTGT CCGTTCTGCG CCTGCCCCTT TGTGCCCCTG 
CTCAGCGTCC AGACCCTTGA TTCCCGACCC AGCGCCCCCA GCCCCAAGCC TGCTCCTGCT 
GGTGCCAGCG GCAGCAGCGA CGCTCCTGCC CCCGGGGGCC CGGGCCCTGT GCTCAGTGAC 
CGCAGGCTTT GCCTAGCCCT GGATGAGCCA CAGCTCTGCA ACGGGCACAT GGGGACTGCC 
TCCCGGCGTG TTGAGAGTGG GGCATGGGCG TATCTCAGCC CCCTCGTGCT GCGGAAGGAG 
CTGGAGTCAC TGGTAGAGAA CGAGGGCAGT GAGGTGCTGG CATTGCCTGA GTTGCCTGCC 
GCTCACCCCA TCATCTTCTG GAACCTTCTG TGGTATTTCC AGCGGCTGCG CCTGCCCAGT 
ATTCTGCCGT GCTTGGTGCT GGCCTCCTGT GATGGGCCCC CGCCCCCCCA GGCCCCAGCT 
CCTTGGATAA TGCCTGATCC AGCATCCGTG CAGGTGCGGC TGCTGTGGGA TGTCCTGACC 
CCTGATCCTG ATAGCTGCCC ACCTCTCTAT GTGCTCTGGA GGGTCCACAG GGCATCTACC 
GTGAGATCTT ATTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4B ( XM_006044144.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044206.1 Bubalus bubalis DENN/MADD domain containing 4B (DENND4B), transcript variant X3, mRNA.

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CloneID	OBu03361
Clone ID Related Accession (Same CDS sequence)	XM_006044144.2 , XM_006044144.1
Accession Version	XM_006044144.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	DENN domain-containing protein 4B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783544.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TCGTGATAGC TGGACTTGCA GGGAACGGAG CACCCATCCC TGAGGAAACA 
TGGATTCCTG AACCCAGTGG ACCCCTGCGC CCTCCTCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAAGGC CCTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCCGCGG GGGGCCACCC CTTGGAACTC AGCGCTGGGC TCCTAGGTGG AACTCAACCT 
GTCATCTGCT ACCGGAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ATACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGCG AGGGCACTCC TCATACTTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCCGTGCC CGTCTTCTCC ACCTTTGTGC TCACGGGTGC AGCTGGTGAT 
AAGGTGTACG GTGCTGCCCT GCAGTTCTAC GAGGCGTTCC CGAGGGCCAG GCTGTCGGAG 
CGGCAGGCTC GGGCCCTGGG CCTGCTGAGT GCTGTGGAGC GGGGCCGGGC ACTGGGGGGC 
CGGGCTGTGC GCAGCCGCCG CGCCATCGCT GTGCTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGCGCCT TCCTCACCTT CCTCTACCGC TACTCCGTCT CGGGCCCCCA CCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AATGTTCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGCCAGCC TGTATCCTCA 
CCCCTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGACCCTGGG CCCGGAGCTG 
GCGATCACGC TGCTGCTTGC TGTGCTCACG GAACACAAGC TGCTAGTCCA CTCGCTGCGG 
CCAGATCTGC TCACCAGCGT CTGCGAGGCC CTTGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG TGCCCCCGTG 
CCCTTCATCG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCACC TGTGGATGTC 
ATCTGTGTGG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACCC TGCCCCGCAG ACCCTACAAG GTTCTGCTGG CCACACTGAC ACATCTGTAC 
CAGCAGCTGG ATCAGACGTA TACTGGCCCC GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACGGACTATG AGGCAGTGTG TGGCCGCCGG GCCCGGCTAG AGCGCGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG CCTGCTCAAG GGCTACCGGG ACTTCCTGCG CCCACTCACC 
CAGGCCCCCT CTGAGGGGTC TCGCGATGTT GACAACCTCT TCTTCCTGCA AGGCTTCCTC 
AAGTCCCGAG AACGTTCCAG CCACAAGCTA TACTCCCAGC TGCTACACAC ACAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ACGCTGCCCT TGAATTCTTT 
GACTCTTGTG TCGACAAGGT CCACCCAGAG CAGGAGAAAC CTGAGCCCGT GCCCCTGGTG 
GAGCTGGAGG AGCTGTCGGG CAGTGAGCTC ACCGTGTTTA TCATGCCTCC CGAGGAGCCG 
CCAGCGCCCG AGGGCAGTGA ATCCACTCCC CAGTACTGTT ATGATGGGTT CCCTGAGCTA 
CGGGCTGAGC TGTTCGAGGC TCCTCAAGAA CAAGCCGGGG CTCTGCCTGT GCCAGGCCCT 
TCCCGCAGCG CCCCCAGCAG TCCTGCCCCT CGCCGCACCA AACAGGAGAT GAAGGTGGCG 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTGCTGGGC 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCCTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCAGACGGC CTACCAGGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCTGACG AGGTGTGTTA CCGGGTGCTG ATGCAGCTGT GCTCACACTA CGGGCAGCCC 
GTGCTGTCCG TGCGGGTCAT GCTGGACATG CGGCGGGCGG GCATCGTGCC CAACACCATC 
ACCTATGGCT ACTATAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG CACACCAGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTGGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGCGAG AACAGCGGCA GCGGCAGCAG AAGCGGGAGA AGGTGGAGAC AGCAGCCCAA 
GAGGCAGGCA GCTCGCAGAC AGAGCCCTAT TTGGAGCGCC CCTCCCCTAC CCGCCCGCTT 
CAGCGCCAGA CTACCTGGGC CGGGCGAAGC CTGCGGGACC CCGCCTCACC TATGGGGCGC 
CTGGTGAAGA GTGGAAGCCT GGGGAGTGCC CGAGGGGCAC AGCCCACCGT GGAGGCTGGT 
GTGGCCCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GGGGATCACC CATACCCTGG 
CATGATGGAA GCCTCTCAGA CCTGAGCCTG ACCGGGGAAG AGTCGGCACC CGGAGGCAGC 
CCAGGGGACT CAGGCTCAGC CCTGAGCACC CAGTCCACTG AAACCCTGGA AGGGCCAAGT 
GGGCGGGTGC CCAAGGCTGG TGGGCGTCAA GATGAGGCCA GCACCCCCCG AAGAGGGCTG 
GGCGCCCGCC TCCAACAGCT ACTCACTCCT TCCCGCCGCT CCCCCACCTC TCGTGCCCCC 
CCATCTGAGC TGCCCCCTGA CCTGCCTCCC TCAGCCCGTC GCAGCCCCAT GGACAGCCTT 
CTGCACCCCC GGGAGCGCCC TGGATCCACT GCATCCGAGA GCTCAGCCTC TCTGGGCAGT 
GAGTGGGACC TCTCAGAATC TTCTCTCAGC AGCGTGAGCC TTCGCCGTTC CTCAGAGCGC 
CTCAGTGACA CCCCTGGATC CTTCCAGCCA CCTTCCCTGG AAATTCTGCT GTCTAGCTGC 
TCGCTGTGCC GCGCCTGTGA CTCCCTGGTG TATGATGAGG AGATCATGGC TGGCTGGGCA 
CCTGACGACT CCAACCTCAA CACCACCTGT CCGTTCTGCG CCTGCCCCTT TGTGCCCCTG 
CTCAGCGTCC AGACCCTTGA TTCCCGACCC AGCGCCCCCA GCCCCAAGCC TGCTCCTGCT 
GGTGCCAGCG GCAGCAGCGA CGCTCCTGCC CCCGGGGGCC CGGGCCCTGT GCTCAGTGAC 
CGCAGGCTTT GCCTAGCCCT GGATGAGCCA CAGCTCTGCA ACGGGCACAT GGGGACTGCC 
TCCCGGCGTG TTGAGAGTGG GGCATGGGCG TATCTCAGCC CCCTCGTGCT GCGGAAGGAG 
CTGGAGTCAC TGGTAGAGAA CGAGGGCAGT GAGGTGCTGG CATTGCCTGA GTTGCCTGCC 
GCTCACCCCA TCATCTTCTG GAACCTTCTG TGGTATTTCC AGCGGCTGCG CCTGCCCAGT 
ATTCTGCCGT GCTTGGTGCT GGCCTCCTGT GATGGGCCCC CGCCCCCCCA GGCCCCAGCT 
CCTTGGATAA TGCCTGATCC AGCATCCGTG CAGGTGCGGC TGCTGTGGGA TGTCCTGACC 
CCTGATCCTG ATAGCTGCCC ACCTCTCTAT GTGCTCTGGA GGGTCCACAG GGCATCTACC 
GTGAGATCTT ATTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044206.1
CDS	1032..5189
Translation	MEADAVSEGGAMAEERPPRLVDYFVIAGLAGNGAPIPEETWIPEPSGPLRPPRPAEPITDVAVIAKALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQTLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPVDVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTHLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFFLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPVPLVELEELSGSELTVFIMPPEEPPAPEGSESTPQYCYDGFPELRAELFEAPQEQAGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALQTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLDMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLREQRQRQQKREKVETAAQEAGSSQTEPYLERPSPTRPLQRQTTWAGRSLRDPASPMGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPIPWHDGSLSDLSLTGEESAPGGSPGDSGSALSTQSTETLEGPSGRVPKAGGRQDEASTPRRGLGARLQQLLTPSRRSPTSRAPPSELPPDLPPSARRSPMDSLLHPRERPGSTASESSASLGSEWDLSESSLSSVSLRRSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKPAPAGASGSSDAPAPGGPGPVLSDRRLCLALDEPQLCNGHMGTASRRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPCLVLASCDGPPPPQAPAPWIMPDPASVQVRLLWDVLTPDPDSCPPLYVLWRVHRASTVRSYS

Target ORF information:

RefSeq Version	XM_006044144.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis DENN/MADD domain containing 4B (DENND4B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044144.1

ORF Insert Sequence:

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TCGTGATAGC TGGACTTGCA GGGAACGGAG CACCCATCCC TGAGGAAACA 
TGGATTCCTG AACCCAGTGG ACCCCTGCGC CCTCCTCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAAGGC CCTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCCGCGG GGGGCCACCC CTTGGAACTC AGCGCTGGGC TCCTAGGTGG AACTCAACCT 
GTCATCTGCT ACCGGAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ATACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGCG AGGGCACTCC TCATACTTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCCGTGCC CGTCTTCTCC ACCTTTGTGC TCACGGGTGC AGCTGGTGAT 
AAGGTGTACG GTGCTGCCCT GCAGTTCTAC GAGGCGTTCC CGAGGGCCAG GCTGTCGGAG 
CGGCAGGCTC GGGCCCTGGG CCTGCTGAGT GCTGTGGAGC GGGGCCGGGC ACTGGGGGGC 
CGGGCTGTGC GCAGCCGCCG CGCCATCGCT GTGCTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGCGCCT TCCTCACCTT CCTCTACCGC TACTCCGTCT CGGGCCCCCA CCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AATGTTCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGCCAGCC TGTATCCTCA 
CCCCTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGACCCTGGG CCCGGAGCTG 
GCGATCACGC TGCTGCTTGC TGTGCTCACG GAACACAAGC TGCTAGTCCA CTCGCTGCGG 
CCAGATCTGC TCACCAGCGT CTGCGAGGCC CTTGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG TGCCCCCGTG 
CCCTTCATCG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCACC TGTGGATGTC 
ATCTGTGTGG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACCC TGCCCCGCAG ACCCTACAAG GTTCTGCTGG CCACACTGAC ACATCTGTAC 
CAGCAGCTGG ATCAGACGTA TACTGGCCCC GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACGGACTATG AGGCAGTGTG TGGCCGCCGG GCCCGGCTAG AGCGCGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG CCTGCTCAAG GGCTACCGGG ACTTCCTGCG CCCACTCACC 
CAGGCCCCCT CTGAGGGGTC TCGCGATGTT GACAACCTCT TCTTCCTGCA AGGCTTCCTC 
AAGTCCCGAG AACGTTCCAG CCACAAGCTA TACTCCCAGC TGCTACACAC ACAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ACGCTGCCCT TGAATTCTTT 
GACTCTTGTG TCGACAAGGT CCACCCAGAG CAGGAGAAAC CTGAGCCCGT GCCCCTGGTG 
GAGCTGGAGG AGCTGTCGGG CAGTGAGCTC ACCGTGTTTA TCATGCCTCC CGAGGAGCCG 
CCAGCGCCCG AGGGCAGTGA ATCCACTCCC CAGTACTGTT ATGATGGGTT CCCTGAGCTA 
CGGGCTGAGC TGTTCGAGGC TCCTCAAGAA CAAGCCGGGG CTCTGCCTGT GCCAGGCCCT 
TCCCGCAGCG CCCCCAGCAG TCCTGCCCCT CGCCGCACCA AACAGGAGAT GAAGGTGGCG 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTGCTGGGC 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCCTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCAGACGGC CTACCAGGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCTGACG AGGTGTGTTA CCGGGTGCTG ATGCAGCTGT GCTCACACTA CGGGCAGCCC 
GTGCTGTCCG TGCGGGTCAT GCTGGACATG CGGCGGGCGG GCATCGTGCC CAACACCATC 
ACCTATGGCT ACTATAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG CACACCAGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTGGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGCGAG AACAGCGGCA GCGGCAGCAG AAGCGGGAGA AGGTGGAGAC AGCAGCCCAA 
GAGGCAGGCA GCTCGCAGAC AGAGCCCTAT TTGGAGCGCC CCTCCCCTAC CCGCCCGCTT 
CAGCGCCAGA CTACCTGGGC CGGGCGAAGC CTGCGGGACC CCGCCTCACC TATGGGGCGC 
CTGGTGAAGA GTGGAAGCCT GGGGAGTGCC CGAGGGGCAC AGCCCACCGT GGAGGCTGGT 
GTGGCCCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GGGGATCACC CATACCCTGG 
CATGATGGAA GCCTCTCAGA CCTGAGCCTG ACCGGGGAAG AGTCGGCACC CGGAGGCAGC 
CCAGGGGACT CAGGCTCAGC CCTGAGCACC CAGTCCACTG AAACCCTGGA AGGGCCAAGT 
GGGCGGGTGC CCAAGGCTGG TGGGCGTCAA GATGAGGCCA GCACCCCCCG AAGAGGGCTG 
GGCGCCCGCC TCCAACAGCT ACTCACTCCT TCCCGCCGCT CCCCCACCTC TCGTGCCCCC 
CCATCTGAGC TGCCCCCTGA CCTGCCTCCC TCAGCCCGTC GCAGCCCCAT GGACAGCCTT 
CTGCACCCCC GGGAGCGCCC TGGATCCACT GCATCCGAGA GCTCAGCCTC TCTGGGCAGT 
GAGTGGGACC TCTCAGAATC TTCTCTCAGC AGCGTGAGCC TTCGCCGTTC CTCAGAGCGC 
CTCAGTGACA CCCCTGGATC CTTCCAGCCA CCTTCCCTGG AAATTCTGCT GTCTAGCTGC 
TCGCTGTGCC GCGCCTGTGA CTCCCTGGTG TATGATGAGG AGATCATGGC TGGCTGGGCA 
CCTGACGACT CCAACCTCAA CACCACCTGT CCGTTCTGCG CCTGCCCCTT TGTGCCCCTG 
CTCAGCGTCC AGACCCTTGA TTCCCGACCC AGCGCCCCCA GCCCCAAGCC TGCTCCTGCT 
GGTGCCAGCG GCAGCAGCGA CGCTCCTGCC CCCGGGGGCC CGGGCCCTGT GCTCAGTGAC 
CGCAGGCTTT GCCTAGCCCT GGATGAGCCA CAGCTCTGCA ACGGGCACAT GGGGACTGCC 
TCCCGGCGTG TTGAGAGTGG GGCATGGGCG TATCTCAGCC CCCTCGTGCT GCGGAAGGAG 
CTGGAGTCAC TGGTAGAGAA CGAGGGCAGT GAGGTGCTGG CATTGCCTGA GTTGCCTGCC 
GCTCACCCCA TCATCTTCTG GAACCTTCTG TGGTATTTCC AGCGGCTGCG CCTGCCCAGT 
ATTCTGCCGT GCTTGGTGCT GGCCTCCTGT GATGGGCCCC CGCCCCCCCA GGCCCCAGCT 
CCTTGGATAA TGCCTGATCC AGCATCCGTG CAGGTGCGGC TGCTGTGGGA TGTCCTGACC 
CCTGATCCTG ATAGCTGCCC ACCTCTCTAT GTGCTCTGGA GGGTCCACAG GGCATCTACC 
GTGAGATCTT ATTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4B ( XM_025287791.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025143576.1 Bubalus bubalis DENN domain containing 4B (DENND4B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu68017
Clone ID Related Accession (Same CDS sequence)	XM_025287791.1 , XM_025287790.1
Accession Version	XM_025287791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	DENN domain-containing protein 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG AGCGGCCCCC CCGGCTGGTG GATTACTTCG TGATAGCTGG ACTTGCAGGG 
AACGGAGCAC CCATCCCTGA GGAAACATGG ATTCCTGAAC CCAGTGGACC CCTGCGCCCT 
CCTCGGCCAG CTGAGCCCAT CACAGATGTG GCAGTCATCG CTAAGGCCCT GGGCGAGGAG 
GTGCCCCAGG GCTACACATG CATCCAGGCT TCCGCGGGGG GCCACCCCTT GGAACTCAGC 
GCTGGGCTCC TAGGTGGAAC TCAACCTGTC ATCTGCTACC GGAGGGGGCG TGACAAGCCC 
CCCCTCGTTG AGCTGGGGGT GTTGTATGAG GGGAAGGAAC GTCCCAAGCC TGGCTTCCAA 
GTGCTAGATA CGACACCCTA CAGCCACTCA GCCAACCTGG CCCCTCCAGG CCCTGGGCAC 
CCCCGCACCT ACCTCACTTA CCGGCGGGCA GCAGAGGGGG CAGGGCTGCA TGCCCTGGGC 
ATCACTGACC TCTGCCTGGT ACTGCCCAGC AAGGGCGAGG GCACTCCTCA TACTTACTGC 
CGATTGCCCC GCAACCTCAA CCCTGGCATG TGGGGCCCAG CAGTGTACCT GTGCTACAAG 
GTGGGCCTGG CCAAGGCCAA CACACTGGTG TATGAGGCAG AGCTGCTGGG CCGCTACCCA 
GAGGAGGACA ATGAGGCGTT CCCGCTGCCC GAGTCAGTGC CCGTCTTCTG CCTGCCCATG 
GGGGCCACTA TCGAGTGCTG GCCTGCCCAG ACCAAGTACC CCGTGCCCGT CTTCTCCACC 
TTTGTGCTCA CGGGTGCAGC TGGTGATAAG GTGTACGGTG CTGCCCTGCA GTTCTACGAG 
GCGTTCCCGA GGGCCAGGCT GTCGGAGCGG CAGGCTCGGG CCCTGGGCCT GCTGAGTGCT 
GTGGAGCGGG GCCGGGCACT GGGGGGCCGG GCTGTGCGCA GCCGCCGCGC CATCGCTGTG 
CTGTCCCGCT GGCCTGCCTT CCCAGCCTTC CGCGCCTTCC TCACCTTCCT CTACCGCTAC 
TCCGTCTCGG GCCCCCACCG TCTGCCCCTG GAAGCGCACA TCTCCCACTT CATTCACAAT 
GTTCCCTTCC CTTCCCCACA GAGACCCCGC ATCCTGGTGC AGATGTCTCC CTATGACAAC 
CTGCTCCTCT GCCAGCCTGT ATCCTCACCC CTGCCCCTCA GTGGTGCCAG CTTCCTGCAG 
CTGCTGCAGA CCCTGGGCCC GGAGCTGGCG ATCACGCTGC TGCTTGCTGT GCTCACGGAA 
CACAAGCTGC TAGTCCACTC GCTGCGGCCA GATCTGCTCA CCAGCGTCTG CGAGGCCCTT 
GTCTCTATGA TCTTCCCGCT GCACTGGCAG TGCCCCTACA TTCCGCTGTG CCCACTGGTG 
CTGGCAGATG TGCTGAGTGC CCCCGTGCCC TTCATCGTGG GTATCCACTC CAGCTACTTC 
GATCTGCATG ACCCACCTGT GGATGTCATC TGTGTGGATC TTGATACCAA CACACTCTTC 
CAGACTGAGG AAAAGAAGCC CCTCTCCCCT CGGACCCTGC CCCGCAGACC CTACAAGGTT 
CTGCTGGCCA CACTGACACA TCTGTACCAG CAGCTGGATC AGACGTATAC TGGCCCCGAG 
GAGGAGGCCT CCCTGGAATT CCTGCTGACG GACTATGAGG CAGTGTGTGG CCGCCGGGCC 
CGGCTAGAGC GCGAGGTCCA GGGAGCCTTC CTCCGCTTCA TGGCCTGCCT GCTCAAGGGC 
TACCGGGACT TCCTGCGCCC ACTCACCCAG GCCCCCTCTG AGGGGTCTCG CGATGTTGAC 
AACCTCTTCT TCCTGCAAGG CTTCCTCAAG TCCCGAGAAC GTTCCAGCCA CAAGCTATAC 
TCCCAGCTGC TACACACACA GATGTTCTCC CAGTTCATCG AGGAATGCTC TTTTGGCTCT 
GCTCGGCACG CTGCCCTTGA ATTCTTTGAC TCTTGTGTCG ACAAGGTCCA CCCAGAGCAG 
GAGAAACCTG AGCCCGTGCC CCTGGTGGAG CTGGAGGAGC TGTCGGGCAG TGAGCTCACC 
GTGTTTATCA TGCCTCCCGA GGAGCCGCCA GCGCCCGAGG GCAGTGAATC CACTCCCCAG 
TACTGTTATG ATGGGTTCCC TGAGCTACGG GCTGAGCTGT TCGAGGCTCC TCAAGAACAA 
GCCGGGGCTC TGCCTGTGCC AGGCCCTTCC CGCAGCGCCC CCAGCAGTCC TGCCCCTCGC 
CGCACCAAAC AGGAGATGAA GGTGGCGCAG CGGATGGCAC AGAAGTCAGC AGCTGTGCCT 
GAGCTGTGGG CCCGGTGCCT GCTGGGCCAC TGCTACGGGC TGTGGTTCCT GTGTCTGCCT 
GCCTACGTGC GCTCGGCGCC CTCCCGGGTG CGGGCACTGC AGACGGCCTA CCAGGTGCTG 
CGCCAGATGG AGAGCCGCAA GGTGGTGCTG CCTGACGAGG TGTGTTACCG GGTGCTGATG 
CAGCTGTGCT CACACTACGG GCAGCCCGTG CTGTCCGTGC GGGTCATGCT GGACATGCGG 
CGGGCGGGCA TCGTGCCCAA CACCATCACC TATGGCTACT ATAACAAGGC TGTGCTGGAA 
AGCAAGTGGC CGTCTGGCAC ACCAGGTGGG CGCCTGCGCT GGGCCAAGCT CCGGAACGTG 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTGCGAGAAC AGCGGCAGCG GCAGCAGAAG 
CGGGAGAAGG TGGAGACAGC AGCCCAAGAG GCAGGCAGCT CGCAGACAGA GCCCTATTTG 
GAGCGCCCCT CCCCTACCCG CCCGCTTCAG CGCCAGACTA CCTGGGCCGG GCGAAGCCTG 
CGGGACCCCG CCTCACCTAT GGGGCGCCTG GTGAAGAGTG GAAGCCTGGG GAGTGCCCGA 
GGGGCACAGC CCACCGTGGA GGCTGGTGTG GCCCACATGA TAGAGGCCTT GGGGGTCCTG 
GAACCCCGGG GATCACCCAT ACCCTGGCAT GATGGAAGCC TCTCAGACCT GAGCCTGACC 
GGGGAAGAGT CGGCACCCGG AGGCAGCCCA GGGGACTCAG GCTCAGCCCT GAGCACCCAG 
TCCACTGAAA CCCTGGAAGG GCCAAGTGGG CGGGTGCCCA AGGCTGGTGG GCGTCAAGAT 
GAGGCCAGCA CCCCCCGAAG AGGGCTGGGC GCCCGCCTCC AACAGCTACT CACTCCTTCC 
CGCCGCTCCC CCACCTCTCG TGCCCCCCCA TCTGAGCTGC CCCCTGACCT GCCTCCCTCA 
GCCCGTCGCA GCCCCATGGA CAGCCTTCTG CACCCCCGGG AGCGCCCTGG ATCCACTGCA 
TCCGAGAGCT CAGCCTCTCT GGGCAGTGAG TGGGACCTCT CAGAATCTTC TCTCAGCAGC 
GTGAGCCTTC GCCGTTCCTC AGAGCGCCTC AGTGACACCC CTGGATCCTT CCAGCCACCT 
TCCCTGGAAA TTCTGCTGTC TAGCTGCTCG CTGTGCCGCG CCTGTGACTC CCTGGTGTAT 
GATGAGGAGA TCATGGCTGG CTGGGCACCT GACGACTCCA ACCTCAACAC CACCTGTCCG 
TTCTGCGCCT GCCCCTTTGT GCCCCTGCTC AGCGTCCAGA CCCTTGATTC CCGACCCAGC 
GCCCCCAGCC CCAAGCCTGC TCCTGCTGGT GCCAGCGGCA GCAGCGACGC TCCTGCCCCC 
GGGGGCCCGG GCCCTGTGCT CAGTGACCGC AGGCTTTGCC TAGCCCTGGA TGAGCCACAG 
CTCTGCAACG GGCACATGGG GACTGCCTCC CGGCGTGTTG AGAGTGGGGC ATGGGCGTAT 
CTCAGCCCCC TCGTGCTGCG GAAGGAGCTG GAGTCACTGG TAGAGAACGA GGGCAGTGAG 
GTGCTGGCAT TGCCTGAGTT GCCTGCCGCT CACCCCATCA TCTTCTGGAA CCTTCTGTGG 
TATTTCCAGC GGCTGCGCCT GCCCAGTATT CTGCCGTGCT TGGTGCTGGC CTCCTGTGAT 
GGGCCCCCGC CCCCCCAGGC CCCAGCTCCT TGGATAATGC CTGATCCAGC ATCCGTGCAG 
GTGCGGCTGC TGTGGGATGT CCTGACCCCT GATCCTGATA GCTGCCCACC TCTCTATGTG 
CTCTGGAGGG TCCACAGCCA GATCCCACAG CGGGTGGTAT GGCCAGGTCC AGTACCTGCA 
TCCCTTAGCC TGGCGTTGCT GGAATCGGTG CTGCGCCACG TTGGTCTCAA CGAGGTGCAC 
AAGGCTATAG GGCTCCTGCT AGAAACTCTA GGGCCCCCTC CCACTGGCCT GCACCTACAA 
AGGGGCATCT ACCGTGAGAT CTTATTCCTG ACACTGGCTG CTCTGGGCAA GGACCACATG 
GATATAGTGG CCTTCGACAA GAAGTACAAA TCCGCCTTTA ACAAGCTGGC CAGCAGCATG 
GGCAAGGAGG AGCTGAGGCA GCGGCGGGCA CAGATGCCTA CCCCAAAGGC CATTGATTGC 
CGGAAATGTT TCGGAGCGCC TCTGGAATGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025143576.1
CDS	48..4520
Translation	MAEERPPRLVDYFVIAGLAGNGAPIPEETWIPEPSGPLRPPRPAEPITDVAVIAKALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQTLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPVDVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTHLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFFLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPVPLVELEELSGSELTVFIMPPEEPPAPEGSESTPQYCYDGFPELRAELFEAPQEQAGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALQTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLDMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLREQRQRQQKREKVETAAQEAGSSQTEPYLERPSPTRPLQRQTTWAGRSLRDPASPMGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPIPWHDGSLSDLSLTGEESAPGGSPGDSGSALSTQSTETLEGPSGRVPKAGGRQDEASTPRRGLGARLQQLLTPSRRSPTSRAPPSELPPDLPPSARRSPMDSLLHPRERPGSTASESSASLGSEWDLSESSLSSVSLRRSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKPAPAGASGSSDAPAPGGPGPVLSDRRLCLALDEPQLCNGHMGTASRRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPCLVLASCDGPPPPQAPAPWIMPDPASVQVRLLWDVLTPDPDSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVHKAIGLLLETLGPPPTGLHLQRGIYREILFLTLAALGKDHMDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_025287791.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis DENN domain containing 4B (DENND4B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025287791.1

ORF Insert Sequence:

ATGGCGGAGG AGCGGCCCCC CCGGCTGGTG GATTACTTCG TGATAGCTGG ACTTGCAGGG 
AACGGAGCAC CCATCCCTGA GGAAACATGG ATTCCTGAAC CCAGTGGACC CCTGCGCCCT 
CCTCGGCCAG CTGAGCCCAT CACAGATGTG GCAGTCATCG CTAAGGCCCT GGGCGAGGAG 
GTGCCCCAGG GCTACACATG CATCCAGGCT TCCGCGGGGG GCCACCCCTT GGAACTCAGC 
GCTGGGCTCC TAGGTGGAAC TCAACCTGTC ATCTGCTACC GGAGGGGGCG TGACAAGCCC 
CCCCTCGTTG AGCTGGGGGT GTTGTATGAG GGGAAGGAAC GTCCCAAGCC TGGCTTCCAA 
GTGCTAGATA CGACACCCTA CAGCCACTCA GCCAACCTGG CCCCTCCAGG CCCTGGGCAC 
CCCCGCACCT ACCTCACTTA CCGGCGGGCA GCAGAGGGGG CAGGGCTGCA TGCCCTGGGC 
ATCACTGACC TCTGCCTGGT ACTGCCCAGC AAGGGCGAGG GCACTCCTCA TACTTACTGC 
CGATTGCCCC GCAACCTCAA CCCTGGCATG TGGGGCCCAG CAGTGTACCT GTGCTACAAG 
GTGGGCCTGG CCAAGGCCAA CACACTGGTG TATGAGGCAG AGCTGCTGGG CCGCTACCCA 
GAGGAGGACA ATGAGGCGTT CCCGCTGCCC GAGTCAGTGC CCGTCTTCTG CCTGCCCATG 
GGGGCCACTA TCGAGTGCTG GCCTGCCCAG ACCAAGTACC CCGTGCCCGT CTTCTCCACC 
TTTGTGCTCA CGGGTGCAGC TGGTGATAAG GTGTACGGTG CTGCCCTGCA GTTCTACGAG 
GCGTTCCCGA GGGCCAGGCT GTCGGAGCGG CAGGCTCGGG CCCTGGGCCT GCTGAGTGCT 
GTGGAGCGGG GCCGGGCACT GGGGGGCCGG GCTGTGCGCA GCCGCCGCGC CATCGCTGTG 
CTGTCCCGCT GGCCTGCCTT CCCAGCCTTC CGCGCCTTCC TCACCTTCCT CTACCGCTAC 
TCCGTCTCGG GCCCCCACCG TCTGCCCCTG GAAGCGCACA TCTCCCACTT CATTCACAAT 
GTTCCCTTCC CTTCCCCACA GAGACCCCGC ATCCTGGTGC AGATGTCTCC CTATGACAAC 
CTGCTCCTCT GCCAGCCTGT ATCCTCACCC CTGCCCCTCA GTGGTGCCAG CTTCCTGCAG 
CTGCTGCAGA CCCTGGGCCC GGAGCTGGCG ATCACGCTGC TGCTTGCTGT GCTCACGGAA 
CACAAGCTGC TAGTCCACTC GCTGCGGCCA GATCTGCTCA CCAGCGTCTG CGAGGCCCTT 
GTCTCTATGA TCTTCCCGCT GCACTGGCAG TGCCCCTACA TTCCGCTGTG CCCACTGGTG 
CTGGCAGATG TGCTGAGTGC CCCCGTGCCC TTCATCGTGG GTATCCACTC CAGCTACTTC 
GATCTGCATG ACCCACCTGT GGATGTCATC TGTGTGGATC TTGATACCAA CACACTCTTC 
CAGACTGAGG AAAAGAAGCC CCTCTCCCCT CGGACCCTGC CCCGCAGACC CTACAAGGTT 
CTGCTGGCCA CACTGACACA TCTGTACCAG CAGCTGGATC AGACGTATAC TGGCCCCGAG 
GAGGAGGCCT CCCTGGAATT CCTGCTGACG GACTATGAGG CAGTGTGTGG CCGCCGGGCC 
CGGCTAGAGC GCGAGGTCCA GGGAGCCTTC CTCCGCTTCA TGGCCTGCCT GCTCAAGGGC 
TACCGGGACT TCCTGCGCCC ACTCACCCAG GCCCCCTCTG AGGGGTCTCG CGATGTTGAC 
AACCTCTTCT TCCTGCAAGG CTTCCTCAAG TCCCGAGAAC GTTCCAGCCA CAAGCTATAC 
TCCCAGCTGC TACACACACA GATGTTCTCC CAGTTCATCG AGGAATGCTC TTTTGGCTCT 
GCTCGGCACG CTGCCCTTGA ATTCTTTGAC TCTTGTGTCG ACAAGGTCCA CCCAGAGCAG 
GAGAAACCTG AGCCCGTGCC CCTGGTGGAG CTGGAGGAGC TGTCGGGCAG TGAGCTCACC 
GTGTTTATCA TGCCTCCCGA GGAGCCGCCA GCGCCCGAGG GCAGTGAATC CACTCCCCAG 
TACTGTTATG ATGGGTTCCC TGAGCTACGG GCTGAGCTGT TCGAGGCTCC TCAAGAACAA 
GCCGGGGCTC TGCCTGTGCC AGGCCCTTCC CGCAGCGCCC CCAGCAGTCC TGCCCCTCGC 
CGCACCAAAC AGGAGATGAA GGTGGCGCAG CGGATGGCAC AGAAGTCAGC AGCTGTGCCT 
GAGCTGTGGG CCCGGTGCCT GCTGGGCCAC TGCTACGGGC TGTGGTTCCT GTGTCTGCCT 
GCCTACGTGC GCTCGGCGCC CTCCCGGGTG CGGGCACTGC AGACGGCCTA CCAGGTGCTG 
CGCCAGATGG AGAGCCGCAA GGTGGTGCTG CCTGACGAGG TGTGTTACCG GGTGCTGATG 
CAGCTGTGCT CACACTACGG GCAGCCCGTG CTGTCCGTGC GGGTCATGCT GGACATGCGG 
CGGGCGGGCA TCGTGCCCAA CACCATCACC TATGGCTACT ATAACAAGGC TGTGCTGGAA 
AGCAAGTGGC CGTCTGGCAC ACCAGGTGGG CGCCTGCGCT GGGCCAAGCT CCGGAACGTG 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTGCGAGAAC AGCGGCAGCG GCAGCAGAAG 
CGGGAGAAGG TGGAGACAGC AGCCCAAGAG GCAGGCAGCT CGCAGACAGA GCCCTATTTG 
GAGCGCCCCT CCCCTACCCG CCCGCTTCAG CGCCAGACTA CCTGGGCCGG GCGAAGCCTG 
CGGGACCCCG CCTCACCTAT GGGGCGCCTG GTGAAGAGTG GAAGCCTGGG GAGTGCCCGA 
GGGGCACAGC CCACCGTGGA GGCTGGTGTG GCCCACATGA TAGAGGCCTT GGGGGTCCTG 
GAACCCCGGG GATCACCCAT ACCCTGGCAT GATGGAAGCC TCTCAGACCT GAGCCTGACC 
GGGGAAGAGT CGGCACCCGG AGGCAGCCCA GGGGACTCAG GCTCAGCCCT GAGCACCCAG 
TCCACTGAAA CCCTGGAAGG GCCAAGTGGG CGGGTGCCCA AGGCTGGTGG GCGTCAAGAT 
GAGGCCAGCA CCCCCCGAAG AGGGCTGGGC GCCCGCCTCC AACAGCTACT CACTCCTTCC 
CGCCGCTCCC CCACCTCTCG TGCCCCCCCA TCTGAGCTGC CCCCTGACCT GCCTCCCTCA 
GCCCGTCGCA GCCCCATGGA CAGCCTTCTG CACCCCCGGG AGCGCCCTGG ATCCACTGCA 
TCCGAGAGCT CAGCCTCTCT GGGCAGTGAG TGGGACCTCT CAGAATCTTC TCTCAGCAGC 
GTGAGCCTTC GCCGTTCCTC AGAGCGCCTC AGTGACACCC CTGGATCCTT CCAGCCACCT 
TCCCTGGAAA TTCTGCTGTC TAGCTGCTCG CTGTGCCGCG CCTGTGACTC CCTGGTGTAT 
GATGAGGAGA TCATGGCTGG CTGGGCACCT GACGACTCCA ACCTCAACAC CACCTGTCCG 
TTCTGCGCCT GCCCCTTTGT GCCCCTGCTC AGCGTCCAGA CCCTTGATTC CCGACCCAGC 
GCCCCCAGCC CCAAGCCTGC TCCTGCTGGT GCCAGCGGCA GCAGCGACGC TCCTGCCCCC 
GGGGGCCCGG GCCCTGTGCT CAGTGACCGC AGGCTTTGCC TAGCCCTGGA TGAGCCACAG 
CTCTGCAACG GGCACATGGG GACTGCCTCC CGGCGTGTTG AGAGTGGGGC ATGGGCGTAT 
CTCAGCCCCC TCGTGCTGCG GAAGGAGCTG GAGTCACTGG TAGAGAACGA GGGCAGTGAG 
GTGCTGGCAT TGCCTGAGTT GCCTGCCGCT CACCCCATCA TCTTCTGGAA CCTTCTGTGG 
TATTTCCAGC GGCTGCGCCT GCCCAGTATT CTGCCGTGCT TGGTGCTGGC CTCCTGTGAT 
GGGCCCCCGC CCCCCCAGGC CCCAGCTCCT TGGATAATGC CTGATCCAGC ATCCGTGCAG 
GTGCGGCTGC TGTGGGATGT CCTGACCCCT GATCCTGATA GCTGCCCACC TCTCTATGTG 
CTCTGGAGGG TCCACAGCCA GATCCCACAG CGGGTGGTAT GGCCAGGTCC AGTACCTGCA 
TCCCTTAGCC TGGCGTTGCT GGAATCGGTG CTGCGCCACG TTGGTCTCAA CGAGGTGCAC 
AAGGCTATAG GGCTCCTGCT AGAAACTCTA GGGCCCCCTC CCACTGGCCT GCACCTACAA 
AGGGGCATCT ACCGTGAGAT CTTATTCCTG ACACTGGCTG CTCTGGGCAA GGACCACATG 
GATATAGTGG CCTTCGACAA GAAGTACAAA TCCGCCTTTA ACAAGCTGGC CAGCAGCATG 
GGCAAGGAGG AGCTGAGGCA GCGGCGGGCA CAGATGCCTA CCCCAAAGGC CATTGATTGC 
CGGAAATGTT TCGGAGCGCC TCTGGAATGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4B ( XM_025287790.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025143575.1 Bubalus bubalis DENN domain containing 4B (DENND4B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu68017
Clone ID Related Accession (Same CDS sequence)	XM_025287791.1 , XM_025287790.1
Accession Version	XM_025287790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	DENN domain-containing protein 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG AGCGGCCCCC CCGGCTGGTG GATTACTTCG TGATAGCTGG ACTTGCAGGG 
AACGGAGCAC CCATCCCTGA GGAAACATGG ATTCCTGAAC CCAGTGGACC CCTGCGCCCT 
CCTCGGCCAG CTGAGCCCAT CACAGATGTG GCAGTCATCG CTAAGGCCCT GGGCGAGGAG 
GTGCCCCAGG GCTACACATG CATCCAGGCT TCCGCGGGGG GCCACCCCTT GGAACTCAGC 
GCTGGGCTCC TAGGTGGAAC TCAACCTGTC ATCTGCTACC GGAGGGGGCG TGACAAGCCC 
CCCCTCGTTG AGCTGGGGGT GTTGTATGAG GGGAAGGAAC GTCCCAAGCC TGGCTTCCAA 
GTGCTAGATA CGACACCCTA CAGCCACTCA GCCAACCTGG CCCCTCCAGG CCCTGGGCAC 
CCCCGCACCT ACCTCACTTA CCGGCGGGCA GCAGAGGGGG CAGGGCTGCA TGCCCTGGGC 
ATCACTGACC TCTGCCTGGT ACTGCCCAGC AAGGGCGAGG GCACTCCTCA TACTTACTGC 
CGATTGCCCC GCAACCTCAA CCCTGGCATG TGGGGCCCAG CAGTGTACCT GTGCTACAAG 
GTGGGCCTGG CCAAGGCCAA CACACTGGTG TATGAGGCAG AGCTGCTGGG CCGCTACCCA 
GAGGAGGACA ATGAGGCGTT CCCGCTGCCC GAGTCAGTGC CCGTCTTCTG CCTGCCCATG 
GGGGCCACTA TCGAGTGCTG GCCTGCCCAG ACCAAGTACC CCGTGCCCGT CTTCTCCACC 
TTTGTGCTCA CGGGTGCAGC TGGTGATAAG GTGTACGGTG CTGCCCTGCA GTTCTACGAG 
GCGTTCCCGA GGGCCAGGCT GTCGGAGCGG CAGGCTCGGG CCCTGGGCCT GCTGAGTGCT 
GTGGAGCGGG GCCGGGCACT GGGGGGCCGG GCTGTGCGCA GCCGCCGCGC CATCGCTGTG 
CTGTCCCGCT GGCCTGCCTT CCCAGCCTTC CGCGCCTTCC TCACCTTCCT CTACCGCTAC 
TCCGTCTCGG GCCCCCACCG TCTGCCCCTG GAAGCGCACA TCTCCCACTT CATTCACAAT 
GTTCCCTTCC CTTCCCCACA GAGACCCCGC ATCCTGGTGC AGATGTCTCC CTATGACAAC 
CTGCTCCTCT GCCAGCCTGT ATCCTCACCC CTGCCCCTCA GTGGTGCCAG CTTCCTGCAG 
CTGCTGCAGA CCCTGGGCCC GGAGCTGGCG ATCACGCTGC TGCTTGCTGT GCTCACGGAA 
CACAAGCTGC TAGTCCACTC GCTGCGGCCA GATCTGCTCA CCAGCGTCTG CGAGGCCCTT 
GTCTCTATGA TCTTCCCGCT GCACTGGCAG TGCCCCTACA TTCCGCTGTG CCCACTGGTG 
CTGGCAGATG TGCTGAGTGC CCCCGTGCCC TTCATCGTGG GTATCCACTC CAGCTACTTC 
GATCTGCATG ACCCACCTGT GGATGTCATC TGTGTGGATC TTGATACCAA CACACTCTTC 
CAGACTGAGG AAAAGAAGCC CCTCTCCCCT CGGACCCTGC CCCGCAGACC CTACAAGGTT 
CTGCTGGCCA CACTGACACA TCTGTACCAG CAGCTGGATC AGACGTATAC TGGCCCCGAG 
GAGGAGGCCT CCCTGGAATT CCTGCTGACG GACTATGAGG CAGTGTGTGG CCGCCGGGCC 
CGGCTAGAGC GCGAGGTCCA GGGAGCCTTC CTCCGCTTCA TGGCCTGCCT GCTCAAGGGC 
TACCGGGACT TCCTGCGCCC ACTCACCCAG GCCCCCTCTG AGGGGTCTCG CGATGTTGAC 
AACCTCTTCT TCCTGCAAGG CTTCCTCAAG TCCCGAGAAC GTTCCAGCCA CAAGCTATAC 
TCCCAGCTGC TACACACACA GATGTTCTCC CAGTTCATCG AGGAATGCTC TTTTGGCTCT 
GCTCGGCACG CTGCCCTTGA ATTCTTTGAC TCTTGTGTCG ACAAGGTCCA CCCAGAGCAG 
GAGAAACCTG AGCCCGTGCC CCTGGTGGAG CTGGAGGAGC TGTCGGGCAG TGAGCTCACC 
GTGTTTATCA TGCCTCCCGA GGAGCCGCCA GCGCCCGAGG GCAGTGAATC CACTCCCCAG 
TACTGTTATG ATGGGTTCCC TGAGCTACGG GCTGAGCTGT TCGAGGCTCC TCAAGAACAA 
GCCGGGGCTC TGCCTGTGCC AGGCCCTTCC CGCAGCGCCC CCAGCAGTCC TGCCCCTCGC 
CGCACCAAAC AGGAGATGAA GGTGGCGCAG CGGATGGCAC AGAAGTCAGC AGCTGTGCCT 
GAGCTGTGGG CCCGGTGCCT GCTGGGCCAC TGCTACGGGC TGTGGTTCCT GTGTCTGCCT 
GCCTACGTGC GCTCGGCGCC CTCCCGGGTG CGGGCACTGC AGACGGCCTA CCAGGTGCTG 
CGCCAGATGG AGAGCCGCAA GGTGGTGCTG CCTGACGAGG TGTGTTACCG GGTGCTGATG 
CAGCTGTGCT CACACTACGG GCAGCCCGTG CTGTCCGTGC GGGTCATGCT GGACATGCGG 
CGGGCGGGCA TCGTGCCCAA CACCATCACC TATGGCTACT ATAACAAGGC TGTGCTGGAA 
AGCAAGTGGC CGTCTGGCAC ACCAGGTGGG CGCCTGCGCT GGGCCAAGCT CCGGAACGTG 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTGCGAGAAC AGCGGCAGCG GCAGCAGAAG 
CGGGAGAAGG TGGAGACAGC AGCCCAAGAG GCAGGCAGCT CGCAGACAGA GCCCTATTTG 
GAGCGCCCCT CCCCTACCCG CCCGCTTCAG CGCCAGACTA CCTGGGCCGG GCGAAGCCTG 
CGGGACCCCG CCTCACCTAT GGGGCGCCTG GTGAAGAGTG GAAGCCTGGG GAGTGCCCGA 
GGGGCACAGC CCACCGTGGA GGCTGGTGTG GCCCACATGA TAGAGGCCTT GGGGGTCCTG 
GAACCCCGGG GATCACCCAT ACCCTGGCAT GATGGAAGCC TCTCAGACCT GAGCCTGACC 
GGGGAAGAGT CGGCACCCGG AGGCAGCCCA GGGGACTCAG GCTCAGCCCT GAGCACCCAG 
TCCACTGAAA CCCTGGAAGG GCCAAGTGGG CGGGTGCCCA AGGCTGGTGG GCGTCAAGAT 
GAGGCCAGCA CCCCCCGAAG AGGGCTGGGC GCCCGCCTCC AACAGCTACT CACTCCTTCC 
CGCCGCTCCC CCACCTCTCG TGCCCCCCCA TCTGAGCTGC CCCCTGACCT GCCTCCCTCA 
GCCCGTCGCA GCCCCATGGA CAGCCTTCTG CACCCCCGGG AGCGCCCTGG ATCCACTGCA 
TCCGAGAGCT CAGCCTCTCT GGGCAGTGAG TGGGACCTCT CAGAATCTTC TCTCAGCAGC 
GTGAGCCTTC GCCGTTCCTC AGAGCGCCTC AGTGACACCC CTGGATCCTT CCAGCCACCT 
TCCCTGGAAA TTCTGCTGTC TAGCTGCTCG CTGTGCCGCG CCTGTGACTC CCTGGTGTAT 
GATGAGGAGA TCATGGCTGG CTGGGCACCT GACGACTCCA ACCTCAACAC CACCTGTCCG 
TTCTGCGCCT GCCCCTTTGT GCCCCTGCTC AGCGTCCAGA CCCTTGATTC CCGACCCAGC 
GCCCCCAGCC CCAAGCCTGC TCCTGCTGGT GCCAGCGGCA GCAGCGACGC TCCTGCCCCC 
GGGGGCCCGG GCCCTGTGCT CAGTGACCGC AGGCTTTGCC TAGCCCTGGA TGAGCCACAG 
CTCTGCAACG GGCACATGGG GACTGCCTCC CGGCGTGTTG AGAGTGGGGC ATGGGCGTAT 
CTCAGCCCCC TCGTGCTGCG GAAGGAGCTG GAGTCACTGG TAGAGAACGA GGGCAGTGAG 
GTGCTGGCAT TGCCTGAGTT GCCTGCCGCT CACCCCATCA TCTTCTGGAA CCTTCTGTGG 
TATTTCCAGC GGCTGCGCCT GCCCAGTATT CTGCCGTGCT TGGTGCTGGC CTCCTGTGAT 
GGGCCCCCGC CCCCCCAGGC CCCAGCTCCT TGGATAATGC CTGATCCAGC ATCCGTGCAG 
GTGCGGCTGC TGTGGGATGT CCTGACCCCT GATCCTGATA GCTGCCCACC TCTCTATGTG 
CTCTGGAGGG TCCACAGCCA GATCCCACAG CGGGTGGTAT GGCCAGGTCC AGTACCTGCA 
TCCCTTAGCC TGGCGTTGCT GGAATCGGTG CTGCGCCACG TTGGTCTCAA CGAGGTGCAC 
AAGGCTATAG GGCTCCTGCT AGAAACTCTA GGGCCCCCTC CCACTGGCCT GCACCTACAA 
AGGGGCATCT ACCGTGAGAT CTTATTCCTG ACACTGGCTG CTCTGGGCAA GGACCACATG 
GATATAGTGG CCTTCGACAA GAAGTACAAA TCCGCCTTTA ACAAGCTGGC CAGCAGCATG 
GGCAAGGAGG AGCTGAGGCA GCGGCGGGCA CAGATGCCTA CCCCAAAGGC CATTGATTGC 
CGGAAATGTT TCGGAGCGCC TCTGGAATGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025143575.1
CDS	167..4639
Translation	MAEERPPRLVDYFVIAGLAGNGAPIPEETWIPEPSGPLRPPRPAEPITDVAVIAKALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQTLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPVDVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTHLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFFLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPVPLVELEELSGSELTVFIMPPEEPPAPEGSESTPQYCYDGFPELRAELFEAPQEQAGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALQTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLDMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLREQRQRQQKREKVETAAQEAGSSQTEPYLERPSPTRPLQRQTTWAGRSLRDPASPMGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPIPWHDGSLSDLSLTGEESAPGGSPGDSGSALSTQSTETLEGPSGRVPKAGGRQDEASTPRRGLGARLQQLLTPSRRSPTSRAPPSELPPDLPPSARRSPMDSLLHPRERPGSTASESSASLGSEWDLSESSLSSVSLRRSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKPAPAGASGSSDAPAPGGPGPVLSDRRLCLALDEPQLCNGHMGTASRRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPCLVLASCDGPPPPQAPAPWIMPDPASVQVRLLWDVLTPDPDSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVHKAIGLLLETLGPPPTGLHLQRGIYREILFLTLAALGKDHMDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_025287790.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis DENN domain containing 4B (DENND4B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025287790.1

ORF Insert Sequence:

ATGGCGGAGG AGCGGCCCCC CCGGCTGGTG GATTACTTCG TGATAGCTGG ACTTGCAGGG 
AACGGAGCAC CCATCCCTGA GGAAACATGG ATTCCTGAAC CCAGTGGACC CCTGCGCCCT 
CCTCGGCCAG CTGAGCCCAT CACAGATGTG GCAGTCATCG CTAAGGCCCT GGGCGAGGAG 
GTGCCCCAGG GCTACACATG CATCCAGGCT TCCGCGGGGG GCCACCCCTT GGAACTCAGC 
GCTGGGCTCC TAGGTGGAAC TCAACCTGTC ATCTGCTACC GGAGGGGGCG TGACAAGCCC 
CCCCTCGTTG AGCTGGGGGT GTTGTATGAG GGGAAGGAAC GTCCCAAGCC TGGCTTCCAA 
GTGCTAGATA CGACACCCTA CAGCCACTCA GCCAACCTGG CCCCTCCAGG CCCTGGGCAC 
CCCCGCACCT ACCTCACTTA CCGGCGGGCA GCAGAGGGGG CAGGGCTGCA TGCCCTGGGC 
ATCACTGACC TCTGCCTGGT ACTGCCCAGC AAGGGCGAGG GCACTCCTCA TACTTACTGC 
CGATTGCCCC GCAACCTCAA CCCTGGCATG TGGGGCCCAG CAGTGTACCT GTGCTACAAG 
GTGGGCCTGG CCAAGGCCAA CACACTGGTG TATGAGGCAG AGCTGCTGGG CCGCTACCCA 
GAGGAGGACA ATGAGGCGTT CCCGCTGCCC GAGTCAGTGC CCGTCTTCTG CCTGCCCATG 
GGGGCCACTA TCGAGTGCTG GCCTGCCCAG ACCAAGTACC CCGTGCCCGT CTTCTCCACC 
TTTGTGCTCA CGGGTGCAGC TGGTGATAAG GTGTACGGTG CTGCCCTGCA GTTCTACGAG 
GCGTTCCCGA GGGCCAGGCT GTCGGAGCGG CAGGCTCGGG CCCTGGGCCT GCTGAGTGCT 
GTGGAGCGGG GCCGGGCACT GGGGGGCCGG GCTGTGCGCA GCCGCCGCGC CATCGCTGTG 
CTGTCCCGCT GGCCTGCCTT CCCAGCCTTC CGCGCCTTCC TCACCTTCCT CTACCGCTAC 
TCCGTCTCGG GCCCCCACCG TCTGCCCCTG GAAGCGCACA TCTCCCACTT CATTCACAAT 
GTTCCCTTCC CTTCCCCACA GAGACCCCGC ATCCTGGTGC AGATGTCTCC CTATGACAAC 
CTGCTCCTCT GCCAGCCTGT ATCCTCACCC CTGCCCCTCA GTGGTGCCAG CTTCCTGCAG 
CTGCTGCAGA CCCTGGGCCC GGAGCTGGCG ATCACGCTGC TGCTTGCTGT GCTCACGGAA 
CACAAGCTGC TAGTCCACTC GCTGCGGCCA GATCTGCTCA CCAGCGTCTG CGAGGCCCTT 
GTCTCTATGA TCTTCCCGCT GCACTGGCAG TGCCCCTACA TTCCGCTGTG CCCACTGGTG 
CTGGCAGATG TGCTGAGTGC CCCCGTGCCC TTCATCGTGG GTATCCACTC CAGCTACTTC 
GATCTGCATG ACCCACCTGT GGATGTCATC TGTGTGGATC TTGATACCAA CACACTCTTC 
CAGACTGAGG AAAAGAAGCC CCTCTCCCCT CGGACCCTGC CCCGCAGACC CTACAAGGTT 
CTGCTGGCCA CACTGACACA TCTGTACCAG CAGCTGGATC AGACGTATAC TGGCCCCGAG 
GAGGAGGCCT CCCTGGAATT CCTGCTGACG GACTATGAGG CAGTGTGTGG CCGCCGGGCC 
CGGCTAGAGC GCGAGGTCCA GGGAGCCTTC CTCCGCTTCA TGGCCTGCCT GCTCAAGGGC 
TACCGGGACT TCCTGCGCCC ACTCACCCAG GCCCCCTCTG AGGGGTCTCG CGATGTTGAC 
AACCTCTTCT TCCTGCAAGG CTTCCTCAAG TCCCGAGAAC GTTCCAGCCA CAAGCTATAC 
TCCCAGCTGC TACACACACA GATGTTCTCC CAGTTCATCG AGGAATGCTC TTTTGGCTCT 
GCTCGGCACG CTGCCCTTGA ATTCTTTGAC TCTTGTGTCG ACAAGGTCCA CCCAGAGCAG 
GAGAAACCTG AGCCCGTGCC CCTGGTGGAG CTGGAGGAGC TGTCGGGCAG TGAGCTCACC 
GTGTTTATCA TGCCTCCCGA GGAGCCGCCA GCGCCCGAGG GCAGTGAATC CACTCCCCAG 
TACTGTTATG ATGGGTTCCC TGAGCTACGG GCTGAGCTGT TCGAGGCTCC TCAAGAACAA 
GCCGGGGCTC TGCCTGTGCC AGGCCCTTCC CGCAGCGCCC CCAGCAGTCC TGCCCCTCGC 
CGCACCAAAC AGGAGATGAA GGTGGCGCAG CGGATGGCAC AGAAGTCAGC AGCTGTGCCT 
GAGCTGTGGG CCCGGTGCCT GCTGGGCCAC TGCTACGGGC TGTGGTTCCT GTGTCTGCCT 
GCCTACGTGC GCTCGGCGCC CTCCCGGGTG CGGGCACTGC AGACGGCCTA CCAGGTGCTG 
CGCCAGATGG AGAGCCGCAA GGTGGTGCTG CCTGACGAGG TGTGTTACCG GGTGCTGATG 
CAGCTGTGCT CACACTACGG GCAGCCCGTG CTGTCCGTGC GGGTCATGCT GGACATGCGG 
CGGGCGGGCA TCGTGCCCAA CACCATCACC TATGGCTACT ATAACAAGGC TGTGCTGGAA 
AGCAAGTGGC CGTCTGGCAC ACCAGGTGGG CGCCTGCGCT GGGCCAAGCT CCGGAACGTG 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTGCGAGAAC AGCGGCAGCG GCAGCAGAAG 
CGGGAGAAGG TGGAGACAGC AGCCCAAGAG GCAGGCAGCT CGCAGACAGA GCCCTATTTG 
GAGCGCCCCT CCCCTACCCG CCCGCTTCAG CGCCAGACTA CCTGGGCCGG GCGAAGCCTG 
CGGGACCCCG CCTCACCTAT GGGGCGCCTG GTGAAGAGTG GAAGCCTGGG GAGTGCCCGA 
GGGGCACAGC CCACCGTGGA GGCTGGTGTG GCCCACATGA TAGAGGCCTT GGGGGTCCTG 
GAACCCCGGG GATCACCCAT ACCCTGGCAT GATGGAAGCC TCTCAGACCT GAGCCTGACC 
GGGGAAGAGT CGGCACCCGG AGGCAGCCCA GGGGACTCAG GCTCAGCCCT GAGCACCCAG 
TCCACTGAAA CCCTGGAAGG GCCAAGTGGG CGGGTGCCCA AGGCTGGTGG GCGTCAAGAT 
GAGGCCAGCA CCCCCCGAAG AGGGCTGGGC GCCCGCCTCC AACAGCTACT CACTCCTTCC 
CGCCGCTCCC CCACCTCTCG TGCCCCCCCA TCTGAGCTGC CCCCTGACCT GCCTCCCTCA 
GCCCGTCGCA GCCCCATGGA CAGCCTTCTG CACCCCCGGG AGCGCCCTGG ATCCACTGCA 
TCCGAGAGCT CAGCCTCTCT GGGCAGTGAG TGGGACCTCT CAGAATCTTC TCTCAGCAGC 
GTGAGCCTTC GCCGTTCCTC AGAGCGCCTC AGTGACACCC CTGGATCCTT CCAGCCACCT 
TCCCTGGAAA TTCTGCTGTC TAGCTGCTCG CTGTGCCGCG CCTGTGACTC CCTGGTGTAT 
GATGAGGAGA TCATGGCTGG CTGGGCACCT GACGACTCCA ACCTCAACAC CACCTGTCCG 
TTCTGCGCCT GCCCCTTTGT GCCCCTGCTC AGCGTCCAGA CCCTTGATTC CCGACCCAGC 
GCCCCCAGCC CCAAGCCTGC TCCTGCTGGT GCCAGCGGCA GCAGCGACGC TCCTGCCCCC 
GGGGGCCCGG GCCCTGTGCT CAGTGACCGC AGGCTTTGCC TAGCCCTGGA TGAGCCACAG 
CTCTGCAACG GGCACATGGG GACTGCCTCC CGGCGTGTTG AGAGTGGGGC ATGGGCGTAT 
CTCAGCCCCC TCGTGCTGCG GAAGGAGCTG GAGTCACTGG TAGAGAACGA GGGCAGTGAG 
GTGCTGGCAT TGCCTGAGTT GCCTGCCGCT CACCCCATCA TCTTCTGGAA CCTTCTGTGG 
TATTTCCAGC GGCTGCGCCT GCCCAGTATT CTGCCGTGCT TGGTGCTGGC CTCCTGTGAT 
GGGCCCCCGC CCCCCCAGGC CCCAGCTCCT TGGATAATGC CTGATCCAGC ATCCGTGCAG 
GTGCGGCTGC TGTGGGATGT CCTGACCCCT GATCCTGATA GCTGCCCACC TCTCTATGTG 
CTCTGGAGGG TCCACAGCCA GATCCCACAG CGGGTGGTAT GGCCAGGTCC AGTACCTGCA 
TCCCTTAGCC TGGCGTTGCT GGAATCGGTG CTGCGCCACG TTGGTCTCAA CGAGGTGCAC 
AAGGCTATAG GGCTCCTGCT AGAAACTCTA GGGCCCCCTC CCACTGGCCT GCACCTACAA 
AGGGGCATCT ACCGTGAGAT CTTATTCCTG ACACTGGCTG CTCTGGGCAA GGACCACATG 
GATATAGTGG CCTTCGACAA GAAGTACAAA TCCGCCTTTA ACAAGCTGGC CAGCAGCATG 
GGCAAGGAGG AGCTGAGGCA GCGGCGGGCA CAGATGCCTA CCCCAAAGGC CATTGATTGC 
CGGAAATGTT TCGGAGCGCC TCTGGAATGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4B ( XM_025287792.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025143577.1 Bubalus bubalis DENN domain containing 4B (DENND4B), transcript variant X6, mRNA.

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CloneID	OBu68018
Clone ID Related Accession (Same CDS sequence)	XM_025287792.1
Accession Version	XM_025287792.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	DENN domain-containing protein 4B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCCGTGCC CGTCTTCTCC ACCTTTGTGC TCACGGGTGC AGCTGGTGAT 
AAGGTGTACG GTGCTGCCCT GCAGTTCTAC GAGGCGTTCC CGAGGGCCAG GCTGTCGGAG 
CGGCAGGCTC GGGCCCTGGG CCTGCTGAGT GCTGTGGAGC GGGGCCGGGC ACTGGGGGGC 
CGGGCTGTGC GCAGCCGCCG CGCCATCGCT GTGCTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGCGCCT TCCTCACCTT CCTCTACCGC TACTCCGTCT CGGGCCCCCA CCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AATGTTCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGCCAGCC TGTATCCTCA 
CCCCTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGACCCTGGG CCCGGAGCTG 
GCGATCACGC TGCTGCTTGC TGTGCTCACG GAACACAAGC TGCTAGTCCA CTCGCTGCGG 
CCAGATCTGC TCACCAGCGT CTGCGAGGCC CTTGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG TGCCCCCGTG 
CCCTTCATCG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCACC TGTGGATGTC 
ATCTGTGTGG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACCC TGCCCCGCAG ACCCTACAAG GTTCTGCTGG CCACACTGAC ACATCTGTAC 
CAGCAGCTGG ATCAGACGTA TACTGGCCCC GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACGGACTATG AGGCAGTGTG TGGCCGCCGG GCCCGGCTAG AGCGCGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG CCTGCTCAAG GGCTACCGGG ACTTCCTGCG CCCACTCACC 
CAGGCCCCCT CTGAGGGGTC TCGCGATGTT GACAACCTCT TCTTCCTGCA AGGCTTCCTC 
AAGTCCCGAG AACGTTCCAG CCACAAGCTA TACTCCCAGC TGCTACACAC ACAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ACGCTGCCCT TGAATTCTTT 
GACTCTTGTG TCGACAAGGT CCACCCAGAG CAGGAGAAAC CTGAGCCCGT GCCCCTGGTG 
GAGCTGGAGG AGCTGTCGGG CAGTGAGCTC ACCGTGTTTA TCATGCCTCC CGAGGAGCCG 
CCAGCGCCCG AGGGCAGTGA ATCCACTCCC CAGTACTGTT ATGATGGGTT CCCTGAGCTA 
CGGGCTGAGC TGTTCGAGGC TCCTCAAGAA CAAGCCGGGG CTCTGCCTGT GCCAGGCCCT 
TCCCGCAGCG CCCCCAGCAG TCCTGCCCCT CGCCGCACCA AACAGGAGAT GAAGGTGGCG 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTGCTGGGC 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCCTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCAGACGGC CTACCAGGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCTGACG AGGTGTGTTA CCGGGTGCTG ATGCAGCTGT GCTCACACTA CGGGCAGCCC 
GTGCTGTCCG TGCGGGTCAT GCTGGACATG CGGCGGGCGG GCATCGTGCC CAACACCATC 
ACCTATGGCT ACTATAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG CACACCAGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTGGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGCGAG AACAGCGGCA GCGGCAGCAG AAGCGGGAGA AGGTGGAGAC AGCAGCCCAA 
GAGGCAGGCA GCTCGCAGAC AGAGCCCTAT TTGGAGCGCC CCTCCCCTAC CCGCCCGCTT 
CAGCGCCAGA CTACCTGGGC CGGGCGAAGC CTGCGGGACC CCGCCTCACC TATGGGGCGC 
CTGGTGAAGA GTGGAAGCCT GGGGAGTGCC CGAGGGGCAC AGCCCACCGT GGAGGCTGGT 
GTGGCCCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GGGGATCACC CATACCCTGG 
CATGATGGAA GCCTCTCAGA CCTGAGCCTG ACCGGGGAAG AGTCGGCACC CGGAGGCAGC 
CCAGGGGACT CAGGCTCAGC CCTGAGCACC CAGTCCACTG AAACCCTGGA AGGGCCAAGT 
GGGCGGGTGC CCAAGGCTGG TGGGCGTCAA GATGAGGCCA GCACCCCCCG AAGAGGGCTG 
GGCGCCCGCC TCCAACAGCT ACTCACTCCT TCCCGCCGCT CCCCCACCTC TCGTGCCCCC 
CCATCTGAGC TGCCCCCTGA CCTGCCTCCC TCAGCCCGTC GCAGCCCCAT GGACAGCCTT 
CTGCACCCCC GGGAGCGCCC TGGATCCACT GCATCCGAGA GCTCAGCCTC TCTGGGCAGT 
GAGTGGGACC TCTCAGAATC TTCTCTCAGC AGCGTGAGCC TTCGCCGTTC CTCAGAGCGC 
CTCAGTGACA CCCCTGGATC CTTCCAGCCA CCTTCCCTGG AAATTCTGCT GTCTAGCTGC 
TCGCTGTGCC GCGCCTGTGA CTCCCTGGTG TATGATGAGG AGATCATGGC TGGCTGGGCA 
CCTGACGACT CCAACCTCAA CACCACCTGT CCGTTCTGCG CCTGCCCCTT TGTGCCCCTG 
CTCAGCGTCC AGACCCTTGA TTCCCGACCC AGCGCCCCCA GCCCCAAGCC TGCTCCTGCT 
GGTGCCAGCG GCAGCAGCGA CGCTCCTGCC CCCGGGGGCC CGGGCCCTGT GCTCAGTGAC 
CGCAGGCTTT GCCTAGCCCT GGATGAGCCA CAGCTCTGCA ACGGGCACAT GGGGACTGCC 
TCCCGGCGTG TTGAGAGTGG GGCATGGGCG TATCTCAGCC CCCTCGTGCT GCGGAAGGAG 
CTGGAGTCAC TGGTAGAGAA CGAGGGCAGT GAGGTGCTGG CATTGCCTGA GTTGCCTGCC 
GCTCACCCCA TCATCTTCTG GAACCTTCTG TGGTATTTCC AGCGGCTGCG CCTGCCCAGT 
ATTCTGCCGT GCTTGGTGCT GGCCTCCTGT GATGGGCCCC CGCCCCCCCA GGCCCCAGCT 
CCTTGGATAA TGCCTGATCC AGCATCCGTG CAGGTGCGGC TGCTGTGGGA TGTCCTGACC 
CCTGATCCTG ATAGCTGCCC ACCTCTCTAT GTGCTCTGGA GGGTCCACAG CCAGATCCCA 
CAGCGGGTGG TATGGCCAGG TCCAGTACCT GCATCCCTTA GCCTGGCGTT GCTGGAATCG 
GTGCTGCGCC ACGTTGGTCT CAACGAGGTG CACAAGGCTA TAGGGCTCCT GCTAGAAACT 
CTAGGGCCCC CTCCCACTGG CCTGCACCTA CAAAGGGGCA TCTACCGTGA GATCTTATTC 
CTGACACTGG CTGCTCTGGG CAAGGACCAC ATGGATATAG TGGCCTTCGA CAAGAAGTAC 
AAATCCGCCT TTAACAAGCT GGCCAGCAGC ATGGGCAAGG AGGAGCTGAG GCAGCGGCGG 
GCACAGATGC CTACCCCAAA GGCCATTGAT TGCCGGAAAT GTTTCGGAGC GCCTCTGGAA 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025143577.1
CDS	266..4171
Translation	MWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQTLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPVDVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTHLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFFLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPVPLVELEELSGSELTVFIMPPEEPPAPEGSESTPQYCYDGFPELRAELFEAPQEQAGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALQTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLDMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLREQRQRQQKREKVETAAQEAGSSQTEPYLERPSPTRPLQRQTTWAGRSLRDPASPMGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPIPWHDGSLSDLSLTGEESAPGGSPGDSGSALSTQSTETLEGPSGRVPKAGGRQDEASTPRRGLGARLQQLLTPSRRSPTSRAPPSELPPDLPPSARRSPMDSLLHPRERPGSTASESSASLGSEWDLSESSLSSVSLRRSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKPAPAGASGSSDAPAPGGPGPVLSDRRLCLALDEPQLCNGHMGTASRRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPCLVLASCDGPPPPQAPAPWIMPDPASVQVRLLWDVLTPDPDSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVHKAIGLLLETLGPPPTGLHLQRGIYREILFLTLAALGKDHMDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_025287792.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis DENN domain containing 4B (DENND4B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025287792.1

ORF Insert Sequence:

ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCCGTGCC CGTCTTCTCC ACCTTTGTGC TCACGGGTGC AGCTGGTGAT 
AAGGTGTACG GTGCTGCCCT GCAGTTCTAC GAGGCGTTCC CGAGGGCCAG GCTGTCGGAG 
CGGCAGGCTC GGGCCCTGGG CCTGCTGAGT GCTGTGGAGC GGGGCCGGGC ACTGGGGGGC 
CGGGCTGTGC GCAGCCGCCG CGCCATCGCT GTGCTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGCGCCT TCCTCACCTT CCTCTACCGC TACTCCGTCT CGGGCCCCCA CCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AATGTTCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGCCAGCC TGTATCCTCA 
CCCCTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGACCCTGGG CCCGGAGCTG 
GCGATCACGC TGCTGCTTGC TGTGCTCACG GAACACAAGC TGCTAGTCCA CTCGCTGCGG 
CCAGATCTGC TCACCAGCGT CTGCGAGGCC CTTGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG TGCCCCCGTG 
CCCTTCATCG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCACC TGTGGATGTC 
ATCTGTGTGG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACCC TGCCCCGCAG ACCCTACAAG GTTCTGCTGG CCACACTGAC ACATCTGTAC 
CAGCAGCTGG ATCAGACGTA TACTGGCCCC GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACGGACTATG AGGCAGTGTG TGGCCGCCGG GCCCGGCTAG AGCGCGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG CCTGCTCAAG GGCTACCGGG ACTTCCTGCG CCCACTCACC 
CAGGCCCCCT CTGAGGGGTC TCGCGATGTT GACAACCTCT TCTTCCTGCA AGGCTTCCTC 
AAGTCCCGAG AACGTTCCAG CCACAAGCTA TACTCCCAGC TGCTACACAC ACAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ACGCTGCCCT TGAATTCTTT 
GACTCTTGTG TCGACAAGGT CCACCCAGAG CAGGAGAAAC CTGAGCCCGT GCCCCTGGTG 
GAGCTGGAGG AGCTGTCGGG CAGTGAGCTC ACCGTGTTTA TCATGCCTCC CGAGGAGCCG 
CCAGCGCCCG AGGGCAGTGA ATCCACTCCC CAGTACTGTT ATGATGGGTT CCCTGAGCTA 
CGGGCTGAGC TGTTCGAGGC TCCTCAAGAA CAAGCCGGGG CTCTGCCTGT GCCAGGCCCT 
TCCCGCAGCG CCCCCAGCAG TCCTGCCCCT CGCCGCACCA AACAGGAGAT GAAGGTGGCG 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTGCTGGGC 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCCTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCAGACGGC CTACCAGGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCTGACG AGGTGTGTTA CCGGGTGCTG ATGCAGCTGT GCTCACACTA CGGGCAGCCC 
GTGCTGTCCG TGCGGGTCAT GCTGGACATG CGGCGGGCGG GCATCGTGCC CAACACCATC 
ACCTATGGCT ACTATAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG CACACCAGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTGGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGCGAG AACAGCGGCA GCGGCAGCAG AAGCGGGAGA AGGTGGAGAC AGCAGCCCAA 
GAGGCAGGCA GCTCGCAGAC AGAGCCCTAT TTGGAGCGCC CCTCCCCTAC CCGCCCGCTT 
CAGCGCCAGA CTACCTGGGC CGGGCGAAGC CTGCGGGACC CCGCCTCACC TATGGGGCGC 
CTGGTGAAGA GTGGAAGCCT GGGGAGTGCC CGAGGGGCAC AGCCCACCGT GGAGGCTGGT 
GTGGCCCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GGGGATCACC CATACCCTGG 
CATGATGGAA GCCTCTCAGA CCTGAGCCTG ACCGGGGAAG AGTCGGCACC CGGAGGCAGC 
CCAGGGGACT CAGGCTCAGC CCTGAGCACC CAGTCCACTG AAACCCTGGA AGGGCCAAGT 
GGGCGGGTGC CCAAGGCTGG TGGGCGTCAA GATGAGGCCA GCACCCCCCG AAGAGGGCTG 
GGCGCCCGCC TCCAACAGCT ACTCACTCCT TCCCGCCGCT CCCCCACCTC TCGTGCCCCC 
CCATCTGAGC TGCCCCCTGA CCTGCCTCCC TCAGCCCGTC GCAGCCCCAT GGACAGCCTT 
CTGCACCCCC GGGAGCGCCC TGGATCCACT GCATCCGAGA GCTCAGCCTC TCTGGGCAGT 
GAGTGGGACC TCTCAGAATC TTCTCTCAGC AGCGTGAGCC TTCGCCGTTC CTCAGAGCGC 
CTCAGTGACA CCCCTGGATC CTTCCAGCCA CCTTCCCTGG AAATTCTGCT GTCTAGCTGC 
TCGCTGTGCC GCGCCTGTGA CTCCCTGGTG TATGATGAGG AGATCATGGC TGGCTGGGCA 
CCTGACGACT CCAACCTCAA CACCACCTGT CCGTTCTGCG CCTGCCCCTT TGTGCCCCTG 
CTCAGCGTCC AGACCCTTGA TTCCCGACCC AGCGCCCCCA GCCCCAAGCC TGCTCCTGCT 
GGTGCCAGCG GCAGCAGCGA CGCTCCTGCC CCCGGGGGCC CGGGCCCTGT GCTCAGTGAC 
CGCAGGCTTT GCCTAGCCCT GGATGAGCCA CAGCTCTGCA ACGGGCACAT GGGGACTGCC 
TCCCGGCGTG TTGAGAGTGG GGCATGGGCG TATCTCAGCC CCCTCGTGCT GCGGAAGGAG 
CTGGAGTCAC TGGTAGAGAA CGAGGGCAGT GAGGTGCTGG CATTGCCTGA GTTGCCTGCC 
GCTCACCCCA TCATCTTCTG GAACCTTCTG TGGTATTTCC AGCGGCTGCG CCTGCCCAGT 
ATTCTGCCGT GCTTGGTGCT GGCCTCCTGT GATGGGCCCC CGCCCCCCCA GGCCCCAGCT 
CCTTGGATAA TGCCTGATCC AGCATCCGTG CAGGTGCGGC TGCTGTGGGA TGTCCTGACC 
CCTGATCCTG ATAGCTGCCC ACCTCTCTAT GTGCTCTGGA GGGTCCACAG CCAGATCCCA 
CAGCGGGTGG TATGGCCAGG TCCAGTACCT GCATCCCTTA GCCTGGCGTT GCTGGAATCG 
GTGCTGCGCC ACGTTGGTCT CAACGAGGTG CACAAGGCTA TAGGGCTCCT GCTAGAAACT 
CTAGGGCCCC CTCCCACTGG CCTGCACCTA CAAAGGGGCA TCTACCGTGA GATCTTATTC 
CTGACACTGG CTGCTCTGGG CAAGGACCAC ATGGATATAG TGGCCTTCGA CAAGAAGTAC 
AAATCCGCCT TTAACAAGCT GGCCAGCAGC ATGGGCAAGG AGGAGCTGAG GCAGCGGCGG 
GCACAGATGC CTACCCCAAA GGCCATTGAT TGCCGGAAAT GTTTCGGAGC GCCTCTGGAA 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4B ( XM_006044142.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044204.1 Bubalus bubalis DENN/MADD domain containing 4B (DENND4B), transcript variant X1, mRNA.

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CloneID	OBu03359
Clone ID Related Accession (Same CDS sequence)	XM_006044142.1 , XM_006044142.2
Accession Version	XM_006044142.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	DENN domain-containing protein 4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783544.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TCGTGATAGC TGGACTTGCA GGGAACGGAG CACCCATCCC TGAGGAAACA 
TGGATTCCTG AACCCAGTGG ACCCCTGCGC CCTCCTCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAAGGC CCTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCCGCGG GGGGCCACCC CTTGGAACTC AGCGCTGGGC TCCTAGGTGG AACTCAACCT 
GTCATCTGCT ACCGGAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ATACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGCG AGGGCACTCC TCATACTTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCCGTGCC CGTCTTCTCC ACCTTTGTGC TCACGGGTGC AGCTGGTGAT 
AAGGTGTACG GTGCTGCCCT GCAGTTCTAC GAGGCGTTCC CGAGGGCCAG GCTGTCGGAG 
CGGCAGGCTC GGGCCCTGGG CCTGCTGAGT GCTGTGGAGC GGGGCCGGGC ACTGGGGGGC 
CGGGCTGTGC GCAGCCGCCG CGCCATCGCT GTGCTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGCGCCT TCCTCACCTT CCTCTACCGC TACTCCGTCT CGGGCCCCCA CCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AATGTTCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGCCAGCC TGTATCCTCA 
CCCCTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGACCCTGGG CCCGGAGCTG 
GCGATCACGC TGCTGCTTGC TGTGCTCACG GAACACAAGC TGCTAGTCCA CTCGCTGCGG 
CCAGATCTGC TCACCAGCGT CTGCGAGGCC CTTGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG TGCCCCCGTG 
CCCTTCATCG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCACC TGTGGATGTC 
ATCTGTGTGG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACCC TGCCCCGCAG ACCCTACAAG GTTCTGCTGG CCACACTGAC ACATCTGTAC 
CAGCAGCTGG ATCAGACGTA TACTGGCCCC GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACGGACTATG AGGCAGTGTG TGGCCGCCGG GCCCGGCTAG AGCGCGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG CCTGCTCAAG GGCTACCGGG ACTTCCTGCG CCCACTCACC 
CAGGCCCCCT CTGAGGGGTC TCGCGATGTT GACAACCTCT TCTTCCTGCA AGGCTTCCTC 
AAGTCCCGAG AACGTTCCAG CCACAAGCTA TACTCCCAGC TGCTACACAC ACAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ACGCTGCCCT TGAATTCTTT 
GACTCTTGTG TCGACAAGGT CCACCCAGAG CAGGAGAAAC CTGAGCCCGT GCCCCTGGTG 
GAGCTGGAGG AGCTGTCGGG CAGTGAGCTC ACCGTGTTTA TCATGCCTCC CGAGGAGCCG 
CCAGCGCCCG AGGGCAGTGA ATCCACTCCC CAGTACTGTT ATGATGGGTT CCCTGAGCTA 
CGGGCTGAGC TGTTCGAGGC TCCTCAAGAA CAAGCCGGGG CTCTGCCTGT GCCAGGCCCT 
TCCCGCAGCG CCCCCAGCAG TCCTGCCCCT CGCCGCACCA AACAGGAGAT GAAGGTGGCG 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTGCTGGGC 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCCTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCAGACGGC CTACCAGGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCTGACG AGGTGTGTTA CCGGGTGCTG ATGCAGCTGT GCTCACACTA CGGGCAGCCC 
GTGCTGTCCG TGCGGGTCAT GCTGGACATG CGGCGGGCGG GCATCGTGCC CAACACCATC 
ACCTATGGCT ACTATAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG CACACCAGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTGGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGCGAG AACAGCGGCA GCGGCAGCAG AAGCGGGAGA AGGTGGAGAC AGCAGCCCAA 
GAGGCAGGCA GCTCGCAGAC AGAGCCCTAT TTGGAGCGCC CCTCCCCTAC CCGCCCGCTT 
CAGCGCCAGA CTACCTGGGC CGGGCGAAGC CTGCGGGACC CCGCCTCACC TATGGGGCGC 
CTGGTGAAGA GTGGAAGCCT GGGGAGTGCC CGAGGGGCAC AGCCCACCGT GGAGGCTGGT 
GTGGCCCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GGGGATCACC CATACCCTGG 
CATGATGGAA GCCTCTCAGA CCTGAGCCTG ACCGGGGAAG AGTCGGCACC CGGAGGCAGC 
CCAGGGGACT CAGGCTCAGC CCTGAGCACC CAGTCCACTG AAACCCTGGA AGGGCCAAGT 
GGGCGGGTGC CCAAGGCTGG TGGGCGTCAA GATGAGGCCA GCACCCCCCG AAGAGGGCTG 
GGCGCCCGCC TCCAACAGCT ACTCACTCCT TCCCGCCGCT CCCCCACCTC TCGTGCCCCC 
CCATCTGAGC TGCCCCCTGA CCTGCCTCCC TCAGCCCGTC GCAGCCCCAT GGACAGCCTT 
CTGCACCCCC GGGAGCGCCC TGGATCCACT GCATCCGAGA GCTCAGCCTC TCTGGGCAGT 
GAGTGGGACC TCTCAGAATC TTCTCTCAGC AGCGTGAGCC TTCGCCGTTC CTCAGAGCGC 
CTCAGTGACA CCCCTGGATC CTTCCAGCCA CCTTCCCTGG AAATTCTGCT GTCTAGCTGC 
TCGCTGTGCC GCGCCTGTGA CTCCCTGGTG TATGATGAGG AGATCATGGC TGGCTGGGCA 
CCTGACGACT CCAACCTCAA CACCACCTGT CCGTTCTGCG CCTGCCCCTT TGTGCCCCTG 
CTCAGCGTCC AGACCCTTGA TTCCCGACCC AGCGCCCCCA GCCCCAAGCC TGCTCCTGCT 
GGTGCCAGCG GCAGCAGCGA CGCTCCTGCC CCCGGGGGCC CGGGCCCTGT GCTCAGTGAC 
CGCAGGCTTT GCCTAGCCCT GGATGAGCCA CAGCTCTGCA ACGGGCACAT GGGGACTGCC 
TCCCGGCGTG TTGAGAGTGG GGCATGGGCG TATCTCAGCC CCCTCGTGCT GCGGAAGGAG 
CTGGAGTCAC TGGTAGAGAA CGAGGGCAGT GAGGTGCTGG CATTGCCTGA GTTGCCTGCC 
GCTCACCCCA TCATCTTCTG GAACCTTCTG TGGTATTTCC AGCGGCTGCG CCTGCCCAGT 
ATTCTGCCGT GCTTGGTGCT GGCCTCCTGT GATGGGCCCC CGCCCCCCCA GGCCCCAGCT 
CCTTGGATAA TGCCTGATCC AGCATCCGTG CAGGTGCGGC TGCTGTGGGA TGTCCTGACC 
CCTGATCCTG ATAGCTGCCC ACCTCTCTAT GTGCTCTGGA GGGTCCACAG CCAGATCCCA 
CAGCGGGTGG TATGGCCAGG TCCAGTACCT GCATCCCTTA GCCTGGCGTT GCTGGAATCG 
GTGCTGCGCC ACGTTGGTCT CAACGAGGTG CACAAGGCTA TAGGGCTCCT GCTAGAAACT 
CTAGGGCCCC CTCCCACTGG CCTGCACCTA CAAAGGGGCA TCTACCGTGA GATCTTATTC 
CTGACACTGG CTGCTCTGGG CAAGGACCAC ATGGATATAG TGGCCTTCGA CAAGAAGTAC 
AAATCCGCCT TTAACAAGCT GGCCAGCAGC ATGGGCAAGG AGGAGCTGAG GCAGCGGCGG 
GCACAGATGC CTACCCCAAA GGCCATTGAT TGCCGGAAAT GTTTCGGAGC GCCTCTGGAA 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044204.1
CDS	1030..5535
Translation	MEADAVSEGGAMAEERPPRLVDYFVIAGLAGNGAPIPEETWIPEPSGPLRPPRPAEPITDVAVIAKALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQTLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPVDVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTHLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFFLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPVPLVELEELSGSELTVFIMPPEEPPAPEGSESTPQYCYDGFPELRAELFEAPQEQAGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALQTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLDMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLREQRQRQQKREKVETAAQEAGSSQTEPYLERPSPTRPLQRQTTWAGRSLRDPASPMGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPIPWHDGSLSDLSLTGEESAPGGSPGDSGSALSTQSTETLEGPSGRVPKAGGRQDEASTPRRGLGARLQQLLTPSRRSPTSRAPPSELPPDLPPSARRSPMDSLLHPRERPGSTASESSASLGSEWDLSESSLSSVSLRRSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKPAPAGASGSSDAPAPGGPGPVLSDRRLCLALDEPQLCNGHMGTASRRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPCLVLASCDGPPPPQAPAPWIMPDPASVQVRLLWDVLTPDPDSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVHKAIGLLLETLGPPPTGLHLQRGIYREILFLTLAALGKDHMDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_006044142.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis DENN/MADD domain containing 4B (DENND4B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044142.1

ORF Insert Sequence:

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TCGTGATAGC TGGACTTGCA GGGAACGGAG CACCCATCCC TGAGGAAACA 
TGGATTCCTG AACCCAGTGG ACCCCTGCGC CCTCCTCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAAGGC CCTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCCGCGG GGGGCCACCC CTTGGAACTC AGCGCTGGGC TCCTAGGTGG AACTCAACCT 
GTCATCTGCT ACCGGAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ATACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGCG AGGGCACTCC TCATACTTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCCGTGCC CGTCTTCTCC ACCTTTGTGC TCACGGGTGC AGCTGGTGAT 
AAGGTGTACG GTGCTGCCCT GCAGTTCTAC GAGGCGTTCC CGAGGGCCAG GCTGTCGGAG 
CGGCAGGCTC GGGCCCTGGG CCTGCTGAGT GCTGTGGAGC GGGGCCGGGC ACTGGGGGGC 
CGGGCTGTGC GCAGCCGCCG CGCCATCGCT GTGCTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGCGCCT TCCTCACCTT CCTCTACCGC TACTCCGTCT CGGGCCCCCA CCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AATGTTCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGCCAGCC TGTATCCTCA 
CCCCTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGACCCTGGG CCCGGAGCTG 
GCGATCACGC TGCTGCTTGC TGTGCTCACG GAACACAAGC TGCTAGTCCA CTCGCTGCGG 
CCAGATCTGC TCACCAGCGT CTGCGAGGCC CTTGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG TGCCCCCGTG 
CCCTTCATCG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCACC TGTGGATGTC 
ATCTGTGTGG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACCC TGCCCCGCAG ACCCTACAAG GTTCTGCTGG CCACACTGAC ACATCTGTAC 
CAGCAGCTGG ATCAGACGTA TACTGGCCCC GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACGGACTATG AGGCAGTGTG TGGCCGCCGG GCCCGGCTAG AGCGCGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG CCTGCTCAAG GGCTACCGGG ACTTCCTGCG CCCACTCACC 
CAGGCCCCCT CTGAGGGGTC TCGCGATGTT GACAACCTCT TCTTCCTGCA AGGCTTCCTC 
AAGTCCCGAG AACGTTCCAG CCACAAGCTA TACTCCCAGC TGCTACACAC ACAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ACGCTGCCCT TGAATTCTTT 
GACTCTTGTG TCGACAAGGT CCACCCAGAG CAGGAGAAAC CTGAGCCCGT GCCCCTGGTG 
GAGCTGGAGG AGCTGTCGGG CAGTGAGCTC ACCGTGTTTA TCATGCCTCC CGAGGAGCCG 
CCAGCGCCCG AGGGCAGTGA ATCCACTCCC CAGTACTGTT ATGATGGGTT CCCTGAGCTA 
CGGGCTGAGC TGTTCGAGGC TCCTCAAGAA CAAGCCGGGG CTCTGCCTGT GCCAGGCCCT 
TCCCGCAGCG CCCCCAGCAG TCCTGCCCCT CGCCGCACCA AACAGGAGAT GAAGGTGGCG 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTGCTGGGC 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCCTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCAGACGGC CTACCAGGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCTGACG AGGTGTGTTA CCGGGTGCTG ATGCAGCTGT GCTCACACTA CGGGCAGCCC 
GTGCTGTCCG TGCGGGTCAT GCTGGACATG CGGCGGGCGG GCATCGTGCC CAACACCATC 
ACCTATGGCT ACTATAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG CACACCAGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTGGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGCGAG AACAGCGGCA GCGGCAGCAG AAGCGGGAGA AGGTGGAGAC AGCAGCCCAA 
GAGGCAGGCA GCTCGCAGAC AGAGCCCTAT TTGGAGCGCC CCTCCCCTAC CCGCCCGCTT 
CAGCGCCAGA CTACCTGGGC CGGGCGAAGC CTGCGGGACC CCGCCTCACC TATGGGGCGC 
CTGGTGAAGA GTGGAAGCCT GGGGAGTGCC CGAGGGGCAC AGCCCACCGT GGAGGCTGGT 
GTGGCCCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GGGGATCACC CATACCCTGG 
CATGATGGAA GCCTCTCAGA CCTGAGCCTG ACCGGGGAAG AGTCGGCACC CGGAGGCAGC 
CCAGGGGACT CAGGCTCAGC CCTGAGCACC CAGTCCACTG AAACCCTGGA AGGGCCAAGT 
GGGCGGGTGC CCAAGGCTGG TGGGCGTCAA GATGAGGCCA GCACCCCCCG AAGAGGGCTG 
GGCGCCCGCC TCCAACAGCT ACTCACTCCT TCCCGCCGCT CCCCCACCTC TCGTGCCCCC 
CCATCTGAGC TGCCCCCTGA CCTGCCTCCC TCAGCCCGTC GCAGCCCCAT GGACAGCCTT 
CTGCACCCCC GGGAGCGCCC TGGATCCACT GCATCCGAGA GCTCAGCCTC TCTGGGCAGT 
GAGTGGGACC TCTCAGAATC TTCTCTCAGC AGCGTGAGCC TTCGCCGTTC CTCAGAGCGC 
CTCAGTGACA CCCCTGGATC CTTCCAGCCA CCTTCCCTGG AAATTCTGCT GTCTAGCTGC 
TCGCTGTGCC GCGCCTGTGA CTCCCTGGTG TATGATGAGG AGATCATGGC TGGCTGGGCA 
CCTGACGACT CCAACCTCAA CACCACCTGT CCGTTCTGCG CCTGCCCCTT TGTGCCCCTG 
CTCAGCGTCC AGACCCTTGA TTCCCGACCC AGCGCCCCCA GCCCCAAGCC TGCTCCTGCT 
GGTGCCAGCG GCAGCAGCGA CGCTCCTGCC CCCGGGGGCC CGGGCCCTGT GCTCAGTGAC 
CGCAGGCTTT GCCTAGCCCT GGATGAGCCA CAGCTCTGCA ACGGGCACAT GGGGACTGCC 
TCCCGGCGTG TTGAGAGTGG GGCATGGGCG TATCTCAGCC CCCTCGTGCT GCGGAAGGAG 
CTGGAGTCAC TGGTAGAGAA CGAGGGCAGT GAGGTGCTGG CATTGCCTGA GTTGCCTGCC 
GCTCACCCCA TCATCTTCTG GAACCTTCTG TGGTATTTCC AGCGGCTGCG CCTGCCCAGT 
ATTCTGCCGT GCTTGGTGCT GGCCTCCTGT GATGGGCCCC CGCCCCCCCA GGCCCCAGCT 
CCTTGGATAA TGCCTGATCC AGCATCCGTG CAGGTGCGGC TGCTGTGGGA TGTCCTGACC 
CCTGATCCTG ATAGCTGCCC ACCTCTCTAT GTGCTCTGGA GGGTCCACAG CCAGATCCCA 
CAGCGGGTGG TATGGCCAGG TCCAGTACCT GCATCCCTTA GCCTGGCGTT GCTGGAATCG 
GTGCTGCGCC ACGTTGGTCT CAACGAGGTG CACAAGGCTA TAGGGCTCCT GCTAGAAACT 
CTAGGGCCCC CTCCCACTGG CCTGCACCTA CAAAGGGGCA TCTACCGTGA GATCTTATTC 
CTGACACTGG CTGCTCTGGG CAAGGACCAC ATGGATATAG TGGCCTTCGA CAAGAAGTAC 
AAATCCGCCT TTAACAAGCT GGCCAGCAGC ATGGGCAAGG AGGAGCTGAG GCAGCGGCGG 
GCACAGATGC CTACCCCAAA GGCCATTGAT TGCCGGAAAT GTTTCGGAGC GCCTCTGGAA 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4B ( XM_006044142.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006044204.1 Bubalus bubalis DENN domain containing 4B (DENND4B), transcript variant X1, mRNA.

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CloneID	OBu03359
Clone ID Related Accession (Same CDS sequence)	XM_006044142.1 , XM_006044142.2
Accession Version	XM_006044142.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	DENN domain-containing protein 4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006044142.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TCGTGATAGC TGGACTTGCA GGGAACGGAG CACCCATCCC TGAGGAAACA 
TGGATTCCTG AACCCAGTGG ACCCCTGCGC CCTCCTCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAAGGC CCTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCCGCGG GGGGCCACCC CTTGGAACTC AGCGCTGGGC TCCTAGGTGG AACTCAACCT 
GTCATCTGCT ACCGGAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ATACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGCG AGGGCACTCC TCATACTTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCCGTGCC CGTCTTCTCC ACCTTTGTGC TCACGGGTGC AGCTGGTGAT 
AAGGTGTACG GTGCTGCCCT GCAGTTCTAC GAGGCGTTCC CGAGGGCCAG GCTGTCGGAG 
CGGCAGGCTC GGGCCCTGGG CCTGCTGAGT GCTGTGGAGC GGGGCCGGGC ACTGGGGGGC 
CGGGCTGTGC GCAGCCGCCG CGCCATCGCT GTGCTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGCGCCT TCCTCACCTT CCTCTACCGC TACTCCGTCT CGGGCCCCCA CCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AATGTTCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGCCAGCC TGTATCCTCA 
CCCCTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGACCCTGGG CCCGGAGCTG 
GCGATCACGC TGCTGCTTGC TGTGCTCACG GAACACAAGC TGCTAGTCCA CTCGCTGCGG 
CCAGATCTGC TCACCAGCGT CTGCGAGGCC CTTGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG TGCCCCCGTG 
CCCTTCATCG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCACC TGTGGATGTC 
ATCTGTGTGG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACCC TGCCCCGCAG ACCCTACAAG GTTCTGCTGG CCACACTGAC ACATCTGTAC 
CAGCAGCTGG ATCAGACGTA TACTGGCCCC GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACGGACTATG AGGCAGTGTG TGGCCGCCGG GCCCGGCTAG AGCGCGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG CCTGCTCAAG GGCTACCGGG ACTTCCTGCG CCCACTCACC 
CAGGCCCCCT CTGAGGGGTC TCGCGATGTT GACAACCTCT TCTTCCTGCA AGGCTTCCTC 
AAGTCCCGAG AACGTTCCAG CCACAAGCTA TACTCCCAGC TGCTACACAC ACAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ACGCTGCCCT TGAATTCTTT 
GACTCTTGTG TCGACAAGGT CCACCCAGAG CAGGAGAAAC CTGAGCCCGT GCCCCTGGTG 
GAGCTGGAGG AGCTGTCGGG CAGTGAGCTC ACCGTGTTTA TCATGCCTCC CGAGGAGCCG 
CCAGCGCCCG AGGGCAGTGA ATCCACTCCC CAGTACTGTT ATGATGGGTT CCCTGAGCTA 
CGGGCTGAGC TGTTCGAGGC TCCTCAAGAA CAAGCCGGGG CTCTGCCTGT GCCAGGCCCT 
TCCCGCAGCG CCCCCAGCAG TCCTGCCCCT CGCCGCACCA AACAGGAGAT GAAGGTGGCG 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTGCTGGGC 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCCTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCAGACGGC CTACCAGGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCTGACG AGGTGTGTTA CCGGGTGCTG ATGCAGCTGT GCTCACACTA CGGGCAGCCC 
GTGCTGTCCG TGCGGGTCAT GCTGGACATG CGGCGGGCGG GCATCGTGCC CAACACCATC 
ACCTATGGCT ACTATAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG CACACCAGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTGGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGCGAG AACAGCGGCA GCGGCAGCAG AAGCGGGAGA AGGTGGAGAC AGCAGCCCAA 
GAGGCAGGCA GCTCGCAGAC AGAGCCCTAT TTGGAGCGCC CCTCCCCTAC CCGCCCGCTT 
CAGCGCCAGA CTACCTGGGC CGGGCGAAGC CTGCGGGACC CCGCCTCACC TATGGGGCGC 
CTGGTGAAGA GTGGAAGCCT GGGGAGTGCC CGAGGGGCAC AGCCCACCGT GGAGGCTGGT 
GTGGCCCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GGGGATCACC CATACCCTGG 
CATGATGGAA GCCTCTCAGA CCTGAGCCTG ACCGGGGAAG AGTCGGCACC CGGAGGCAGC 
CCAGGGGACT CAGGCTCAGC CCTGAGCACC CAGTCCACTG AAACCCTGGA AGGGCCAAGT 
GGGCGGGTGC CCAAGGCTGG TGGGCGTCAA GATGAGGCCA GCACCCCCCG AAGAGGGCTG 
GGCGCCCGCC TCCAACAGCT ACTCACTCCT TCCCGCCGCT CCCCCACCTC TCGTGCCCCC 
CCATCTGAGC TGCCCCCTGA CCTGCCTCCC TCAGCCCGTC GCAGCCCCAT GGACAGCCTT 
CTGCACCCCC GGGAGCGCCC TGGATCCACT GCATCCGAGA GCTCAGCCTC TCTGGGCAGT 
GAGTGGGACC TCTCAGAATC TTCTCTCAGC AGCGTGAGCC TTCGCCGTTC CTCAGAGCGC 
CTCAGTGACA CCCCTGGATC CTTCCAGCCA CCTTCCCTGG AAATTCTGCT GTCTAGCTGC 
TCGCTGTGCC GCGCCTGTGA CTCCCTGGTG TATGATGAGG AGATCATGGC TGGCTGGGCA 
CCTGACGACT CCAACCTCAA CACCACCTGT CCGTTCTGCG CCTGCCCCTT TGTGCCCCTG 
CTCAGCGTCC AGACCCTTGA TTCCCGACCC AGCGCCCCCA GCCCCAAGCC TGCTCCTGCT 
GGTGCCAGCG GCAGCAGCGA CGCTCCTGCC CCCGGGGGCC CGGGCCCTGT GCTCAGTGAC 
CGCAGGCTTT GCCTAGCCCT GGATGAGCCA CAGCTCTGCA ACGGGCACAT GGGGACTGCC 
TCCCGGCGTG TTGAGAGTGG GGCATGGGCG TATCTCAGCC CCCTCGTGCT GCGGAAGGAG 
CTGGAGTCAC TGGTAGAGAA CGAGGGCAGT GAGGTGCTGG CATTGCCTGA GTTGCCTGCC 
GCTCACCCCA TCATCTTCTG GAACCTTCTG TGGTATTTCC AGCGGCTGCG CCTGCCCAGT 
ATTCTGCCGT GCTTGGTGCT GGCCTCCTGT GATGGGCCCC CGCCCCCCCA GGCCCCAGCT 
CCTTGGATAA TGCCTGATCC AGCATCCGTG CAGGTGCGGC TGCTGTGGGA TGTCCTGACC 
CCTGATCCTG ATAGCTGCCC ACCTCTCTAT GTGCTCTGGA GGGTCCACAG CCAGATCCCA 
CAGCGGGTGG TATGGCCAGG TCCAGTACCT GCATCCCTTA GCCTGGCGTT GCTGGAATCG 
GTGCTGCGCC ACGTTGGTCT CAACGAGGTG CACAAGGCTA TAGGGCTCCT GCTAGAAACT 
CTAGGGCCCC CTCCCACTGG CCTGCACCTA CAAAGGGGCA TCTACCGTGA GATCTTATTC 
CTGACACTGG CTGCTCTGGG CAAGGACCAC ATGGATATAG TGGCCTTCGA CAAGAAGTAC 
AAATCCGCCT TTAACAAGCT GGCCAGCAGC ATGGGCAAGG AGGAGCTGAG GCAGCGGCGG 
GCACAGATGC CTACCCCAAA GGCCATTGAT TGCCGGAAAT GTTTCGGAGC GCCTCTGGAA 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006044204.1
CDS	659..5164
Translation	MEADAVSEGGAMAEERPPRLVDYFVIAGLAGNGAPIPEETWIPEPSGPLRPPRPAEPITDVAVIAKALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQTLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPVDVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTHLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFFLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPVPLVELEELSGSELTVFIMPPEEPPAPEGSESTPQYCYDGFPELRAELFEAPQEQAGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALQTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLDMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLREQRQRQQKREKVETAAQEAGSSQTEPYLERPSPTRPLQRQTTWAGRSLRDPASPMGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPIPWHDGSLSDLSLTGEESAPGGSPGDSGSALSTQSTETLEGPSGRVPKAGGRQDEASTPRRGLGARLQQLLTPSRRSPTSRAPPSELPPDLPPSARRSPMDSLLHPRERPGSTASESSASLGSEWDLSESSLSSVSLRRSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKPAPAGASGSSDAPAPGGPGPVLSDRRLCLALDEPQLCNGHMGTASRRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPCLVLASCDGPPPPQAPAPWIMPDPASVQVRLLWDVLTPDPDSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVHKAIGLLLETLGPPPTGLHLQRGIYREILFLTLAALGKDHMDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_006044142.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis DENN domain containing 4B (DENND4B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006044142.2

ORF Insert Sequence:

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TCGTGATAGC TGGACTTGCA GGGAACGGAG CACCCATCCC TGAGGAAACA 
TGGATTCCTG AACCCAGTGG ACCCCTGCGC CCTCCTCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAAGGC CCTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCCGCGG GGGGCCACCC CTTGGAACTC AGCGCTGGGC TCCTAGGTGG AACTCAACCT 
GTCATCTGCT ACCGGAGGGG GCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ATACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCCTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGCG AGGGCACTCC TCATACTTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCCGTGCC CGTCTTCTCC ACCTTTGTGC TCACGGGTGC AGCTGGTGAT 
AAGGTGTACG GTGCTGCCCT GCAGTTCTAC GAGGCGTTCC CGAGGGCCAG GCTGTCGGAG 
CGGCAGGCTC GGGCCCTGGG CCTGCTGAGT GCTGTGGAGC GGGGCCGGGC ACTGGGGGGC 
CGGGCTGTGC GCAGCCGCCG CGCCATCGCT GTGCTGTCCC GCTGGCCTGC CTTCCCAGCC 
TTCCGCGCCT TCCTCACCTT CCTCTACCGC TACTCCGTCT CGGGCCCCCA CCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AATGTTCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGCCAGCC TGTATCCTCA 
CCCCTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGACCCTGGG CCCGGAGCTG 
GCGATCACGC TGCTGCTTGC TGTGCTCACG GAACACAAGC TGCTAGTCCA CTCGCTGCGG 
CCAGATCTGC TCACCAGCGT CTGCGAGGCC CTTGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG TGCCCCCGTG 
CCCTTCATCG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCACC TGTGGATGTC 
ATCTGTGTGG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACCC TGCCCCGCAG ACCCTACAAG GTTCTGCTGG CCACACTGAC ACATCTGTAC 
CAGCAGCTGG ATCAGACGTA TACTGGCCCC GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACGGACTATG AGGCAGTGTG TGGCCGCCGG GCCCGGCTAG AGCGCGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG CCTGCTCAAG GGCTACCGGG ACTTCCTGCG CCCACTCACC 
CAGGCCCCCT CTGAGGGGTC TCGCGATGTT GACAACCTCT TCTTCCTGCA AGGCTTCCTC 
AAGTCCCGAG AACGTTCCAG CCACAAGCTA TACTCCCAGC TGCTACACAC ACAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ACGCTGCCCT TGAATTCTTT 
GACTCTTGTG TCGACAAGGT CCACCCAGAG CAGGAGAAAC CTGAGCCCGT GCCCCTGGTG 
GAGCTGGAGG AGCTGTCGGG CAGTGAGCTC ACCGTGTTTA TCATGCCTCC CGAGGAGCCG 
CCAGCGCCCG AGGGCAGTGA ATCCACTCCC CAGTACTGTT ATGATGGGTT CCCTGAGCTA 
CGGGCTGAGC TGTTCGAGGC TCCTCAAGAA CAAGCCGGGG CTCTGCCTGT GCCAGGCCCT 
TCCCGCAGCG CCCCCAGCAG TCCTGCCCCT CGCCGCACCA AACAGGAGAT GAAGGTGGCG 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTGCTGGGC 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCCTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCAGACGGC CTACCAGGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCTGACG AGGTGTGTTA CCGGGTGCTG ATGCAGCTGT GCTCACACTA CGGGCAGCCC 
GTGCTGTCCG TGCGGGTCAT GCTGGACATG CGGCGGGCGG GCATCGTGCC CAACACCATC 
ACCTATGGCT ACTATAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG CACACCAGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTGGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGCGAG AACAGCGGCA GCGGCAGCAG AAGCGGGAGA AGGTGGAGAC AGCAGCCCAA 
GAGGCAGGCA GCTCGCAGAC AGAGCCCTAT TTGGAGCGCC CCTCCCCTAC CCGCCCGCTT 
CAGCGCCAGA CTACCTGGGC CGGGCGAAGC CTGCGGGACC CCGCCTCACC TATGGGGCGC 
CTGGTGAAGA GTGGAAGCCT GGGGAGTGCC CGAGGGGCAC AGCCCACCGT GGAGGCTGGT 
GTGGCCCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GGGGATCACC CATACCCTGG 
CATGATGGAA GCCTCTCAGA CCTGAGCCTG ACCGGGGAAG AGTCGGCACC CGGAGGCAGC 
CCAGGGGACT CAGGCTCAGC CCTGAGCACC CAGTCCACTG AAACCCTGGA AGGGCCAAGT 
GGGCGGGTGC CCAAGGCTGG TGGGCGTCAA GATGAGGCCA GCACCCCCCG AAGAGGGCTG 
GGCGCCCGCC TCCAACAGCT ACTCACTCCT TCCCGCCGCT CCCCCACCTC TCGTGCCCCC 
CCATCTGAGC TGCCCCCTGA CCTGCCTCCC TCAGCCCGTC GCAGCCCCAT GGACAGCCTT 
CTGCACCCCC GGGAGCGCCC TGGATCCACT GCATCCGAGA GCTCAGCCTC TCTGGGCAGT 
GAGTGGGACC TCTCAGAATC TTCTCTCAGC AGCGTGAGCC TTCGCCGTTC CTCAGAGCGC 
CTCAGTGACA CCCCTGGATC CTTCCAGCCA CCTTCCCTGG AAATTCTGCT GTCTAGCTGC 
TCGCTGTGCC GCGCCTGTGA CTCCCTGGTG TATGATGAGG AGATCATGGC TGGCTGGGCA 
CCTGACGACT CCAACCTCAA CACCACCTGT CCGTTCTGCG CCTGCCCCTT TGTGCCCCTG 
CTCAGCGTCC AGACCCTTGA TTCCCGACCC AGCGCCCCCA GCCCCAAGCC TGCTCCTGCT 
GGTGCCAGCG GCAGCAGCGA CGCTCCTGCC CCCGGGGGCC CGGGCCCTGT GCTCAGTGAC 
CGCAGGCTTT GCCTAGCCCT GGATGAGCCA CAGCTCTGCA ACGGGCACAT GGGGACTGCC 
TCCCGGCGTG TTGAGAGTGG GGCATGGGCG TATCTCAGCC CCCTCGTGCT GCGGAAGGAG 
CTGGAGTCAC TGGTAGAGAA CGAGGGCAGT GAGGTGCTGG CATTGCCTGA GTTGCCTGCC 
GCTCACCCCA TCATCTTCTG GAACCTTCTG TGGTATTTCC AGCGGCTGCG CCTGCCCAGT 
ATTCTGCCGT GCTTGGTGCT GGCCTCCTGT GATGGGCCCC CGCCCCCCCA GGCCCCAGCT 
CCTTGGATAA TGCCTGATCC AGCATCCGTG CAGGTGCGGC TGCTGTGGGA TGTCCTGACC 
CCTGATCCTG ATAGCTGCCC ACCTCTCTAT GTGCTCTGGA GGGTCCACAG CCAGATCCCA 
CAGCGGGTGG TATGGCCAGG TCCAGTACCT GCATCCCTTA GCCTGGCGTT GCTGGAATCG 
GTGCTGCGCC ACGTTGGTCT CAACGAGGTG CACAAGGCTA TAGGGCTCCT GCTAGAAACT 
CTAGGGCCCC CTCCCACTGG CCTGCACCTA CAAAGGGGCA TCTACCGTGA GATCTTATTC 
CTGACACTGG CTGCTCTGGG CAAGGACCAC ATGGATATAG TGGCCTTCGA CAAGAAGTAC 
AAATCCGCCT TTAACAAGCT GGCCAGCAGC ATGGGCAAGG AGGAGCTGAG GCAGCGGCGG 
GCACAGATGC CTACCCCAAA GGCCATTGAT TGCCGGAAAT GTTTCGGAGC GCCTCTGGAA 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DENND4B
Entrez Gene ID	102396304
Full Name	DENN domain containing 4B
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DENND4B cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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