SMG7 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following SMG7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMG7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu07047 XM_006048642.1
Latest version!
Bubalus bubalis SMG7 nonsense mediated mRNA decay factor (SMG7), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBu65968 XM_025285494.1
Latest version!
Bubalus bubalis SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu65969 XM_006048642.2
Latest version!
Bubalus bubalis SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu65970 XM_025285495.1
Latest version!
Bubalus bubalis SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu65971 XM_025285496.1
Latest version!
Bubalus bubalis SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu65972 XM_025285498.1
Latest version!
Bubalus bubalis SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu65972 XM_025285497.1
Latest version!
Bubalus bubalis SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu65973 XM_025285500.1
Latest version!
Bubalus bubalis SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu65974 XM_025285501.1
Latest version!
Bubalus bubalis SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu07047
    Clone ID Related Accession (Same CDS sequence) XM_006048642.1
    Accession Version XM_006048642.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product LOW QUALITY PROTEIN: protein SMG7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784136.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC CGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TTGTTACTGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAGGCAAA GAATCGAGCA
    AACCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTGTTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAGTTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTTCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCGGCTCAG
    CTTGTCCCCT CTAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CTACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCTA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGGTG
    AAAACCAAAT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAGAA AGCTTTCAAC TCTCAGCAGT TAGTTCACGT CACTGTCATT
    AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAACG AAACGGAGCA GCATAGTTAT
    AGCCAAGATG AGCAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT CCAGAACAAG TCTCAGGAGG AATCCTACAA TGCCTATCCC
    CTACCTGCCG TCAAGGTGTC CATGGACTGG CTGAGACTCA GACCCAGGGT GTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCGTGGCTAA TTTCTCTTCT GAACAGTTTC
    CATCCCCATG AAGAAGATCT TTCAAGTACT AATGCAACAC CACTTCCAGA GGAGTTTGAG
    TTACAAGGAT TCTTGGCTTT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAT
    CAGGGTATTA CAGGAGACAA AGAGGGTCAG CAACGACAAA TACGACAGCA GCGTTTGATC
    TCTATAGGCA AATGGATTGC AGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAGTTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGACACAACC ATCATAGAGT CCCTGGCAGC TGACGGGAAC
    CCAGGACTGA AATCAGTGCT GTCGACAGGC CGGAATCTAA GCAACAGCTG TGACACGGGA
    GAGAAACCAG TGGTCACCTT CAAAGAGAAC ATTAAGCCCC GAGAAGTGAA CAGAGACCAA
    GGCAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAGT ATCTCTAACT
    AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACAG AAACCAAGAA ATGCACCTTA
    GAAAAGTTAC AGGAAACAGG AAAGCAGAGT GTGGCAGTGC AGGTAAAATC TCAGACAGAG
    TTAAGAAAGA CTCCAGTGTC TGAAGCCAGG AAAACACCTG TAACTCAAAC CCCAAGTCAA
    GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC
    CGGCCAGGGT TCCCGCCCCC AACATATGTT ATCCCCCCTC CTGTGGCATT TTCTATGGGC
    TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT
    CACTCTCCAC CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG
    CAACGGCCCT CCGGACCAGG GCCAGTGACC CAGGGACCTC AGCAGCCTCA GCCACCTTCC
    CAGCAGCCCC TCCCACCTCT GCCAGCTCCG CCAGCAGCAC AGACTGCAGG CCAGCTGCAG
    GTTCAGGCTC TAGCTCAGCA GCAACAGTCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA
    AGTCCTCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG
    TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATCA TGCCTGTGAA ACAGCCCTAC
    TACCTTCAAA CCCAAGACCC CATAAAACTG TTTGAGCCGT CGTTGCAACC TCCTGTAAGG
    CAGCAGCAGC CTCTCGAGAA AACAATGAAG CCTTTCCCCA TGGAGCCATA TAACCATAAC
    CCCTCAGAAG TCAAGATCCC AGAGTTCTAC TGGGATTCTG CCTACAGCGC TGCTGATAAC
    AGAGCTGTGA TGGCGCAGCA AGCCAGCGTG GACCGCAGGG GCAGACGGTC CCCGGGAGTC
    TTCCGTCCAG AGCAGGACCC TGTGCCCAGG ATGCCATTTG AGGATCCCAA AGGCTCCCCT
    CTGCTTCCTC CGGACCTGTT AAAGAGTCTG GCTGCCTTGG AGGAAGAGGA AGAGCTGATT
    TTTTCTAACC CTCCTGATCT TTACCCAGCT CTGCTGGGGC CTCTCGCCTC TCTTCCTGGA
    CGAAGCCTCT TTAAATCCTT ACTGGAGAAG CCCTCAGAGC TCATGTCACA TTCATCCTCT
    TTCCTGTCCC TCACCGGATT CTCCCTCAAT CAGGAACGAT ACCCAAATAA CAGTGTGTTC
    AATGAGGTAT ACGGGAAGAA CCTGACAACC AGCTCCAAAG CAGAACTTAA TCCCTCAATG
    GCTCCCCAGG AAACATCATT GTACTCGCTT TTTGAAGGGA CTCCATGGTC TCCATCTCTT
    CCTGCCAGTT CAGATCATTC AACACCAGCC AGCCAGTCTC CTCATTCCTC CAACCCAAGC
    AGCCTGCCAA GCTCCCCTCC AACACACAAC CACAATTCTG TCCCATTCTC CAATTTTGGA
    CCCATTGGGA CTCCAGATAA CAGGGATAGG AGAACTGCAG ACCGGTGGAA AACTGATAAG
    CCAGCCATGG GTGGGTTTGG CATCGATTAT CTCTCAGCAA CATCATCCTC TGAGAGCAGT
    TGGCATCAGG CCAGCGCTCC AAGTGGCTCC TGGACAGGCC ATGGCCCATC CATGGAGGAT
    TCCTCTGCTG TCCTCATGGA AAGCCTAAAG TCTATCTGGT CCAGTTCCAT GATGCATCCT
    GGACCTTCCG CTTTGGAGCA GCTGTTAATG CAGCAGAAGC AGAAACAGCA GCGGGGACAA
    GGCGCCATGA ACCCTCCACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006048704.1
    CDS142..3705
    Translation

    Target ORF information:

    RefSeq Version XM_006048642.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis SMG7 nonsense mediated mRNA decay factor (SMG7), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006048642.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC CGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TTGTTACTGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAGGCAAA GAATCGAGCA
    AACCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTGTTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAGTTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTTCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCGGCTCAG
    CTTGTCCCCT CTAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CTACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCTA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGGTG
    AAAACCAAAT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAGAA AGCTTTCAAC TCTCAGCAGT TAGTTCACGT CACTGTCATT
    AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAACG AAACGGAGCA GCATAGTTAT
    AGCCAAGATG AGCAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT CCAGAACAAG TCTCAGGAGG AATCCTACAA TGCCTATCCC
    CTACCTGCCG TCAAGGTGTC CATGGACTGG CTGAGACTCA GACCCAGGGT GTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCGTGGCTAA TTTCTCTTCT GAACAGTTTC
    CATCCCCATG AAGAAGATCT TTCAAGTACT AATGCAACAC CACTTCCAGA GGAGTTTGAG
    TTACAAGGAT TCTTGGCTTT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAT
    CAGGGTATTA CAGGAGACAA AGAGGGTCAG CAACGACAAA TACGACAGCA GCGTTTGATC
    TCTATAGGCA AATGGATTGC AGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAGTTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGACACAACC ATCATAGAGT CCCTGGCAGC TGACGGGAAC
    CCAGGACTGA AATCAGTGCT GTCGACAGGC CGGAATCTAA GCAACAGCTG TGACACGGGA
    GAGAAACCAG TGGTCACCTT CAAAGAGAAC ATTAAGCCCC GAGAAGTGAA CAGAGACCAA
    GGCAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAGT ATCTCTAACT
    AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACAG AAACCAAGAA ATGCACCTTA
    GAAAAGTTAC AGGAAACAGG AAAGCAGAGT GTGGCAGTGC AGGTAAAATC TCAGACAGAG
    TTAAGAAAGA CTCCAGTGTC TGAAGCCAGG AAAACACCTG TAACTCAAAC CCCAAGTCAA
    GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC
    CGGCCAGGGT TCCCGCCCCC AACATATGTT ATCCCCCCTC CTGTGGCATT TTCTATGGGC
    TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT
    CACTCTCCAC CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG
    CAACGGCCCT CCGGACCAGG GCCAGTGACC CAGGGACCTC AGCAGCCTCA GCCACCTTCC
    CAGCAGCCCC TCCCACCTCT GCCAGCTCCG CCAGCAGCAC AGACTGCAGG CCAGCTGCAG
    GTTCAGGCTC TAGCTCAGCA GCAACAGTCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA
    AGTCCTCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG
    TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATCA TGCCTGTGAA ACAGCCCTAC
    TACCTTCAAA CCCAAGACCC CATAAAACTG TTTGAGCCGT CGTTGCAACC TCCTGTAAGG
    CAGCAGCAGC CTCTCGAGAA AACAATGAAG CCTTTCCCCA TGGAGCCATA TAACCATAAC
    CCCTCAGAAG TCAAGATCCC AGAGTTCTAC TGGGATTCTG CCTACAGCGC TGCTGATAAC
    AGAGCTGTGA TGGCGCAGCA AGCCAGCGTG GACCGCAGGG GCAGACGGTC CCCGGGAGTC
    TTCCGTCCAG AGCAGGACCC TGTGCCCAGG ATGCCATTTG AGGATCCCAA AGGCTCCCCT
    CTGCTTCCTC CGGACCTGTT AAAGAGTCTG GCTGCCTTGG AGGAAGAGGA AGAGCTGATT
    TTTTCTAACC CTCCTGATCT TTACCCAGCT CTGCTGGGGC CTCTCGCCTC TCTTCCTGGA
    CGAAGCCTCT TTAAATCCTT ACTGGAGAAG CCCTCAGAGC TCATGTCACA TTCATCCTCT
    TTCCTGTCCC TCACCGGATT CTCCCTCAAT CAGGAACGAT ACCCAAATAA CAGTGTGTTC
    AATGAGGTAT ACGGGAAGAA CCTGACAACC AGCTCCAAAG CAGAACTTAA TCCCTCAATG
    GCTCCCCAGG AAACATCATT GTACTCGCTT TTTGAAGGGA CTCCATGGTC TCCATCTCTT
    CCTGCCAGTT CAGATCATTC AACACCAGCC AGCCAGTCTC CTCATTCCTC CAACCCAAGC
    AGCCTGCCAA GCTCCCCTCC AACACACAAC CACAATTCTG TCCCATTCTC CAATTTTGGA
    CCCATTGGGA CTCCAGATAA CAGGGATAGG AGAACTGCAG ACCGGTGGAA AACTGATAAG
    CCAGCCATGG GTGGGTTTGG CATCGATTAT CTCTCAGCAA CATCATCCTC TGAGAGCAGT
    TGGCATCAGG CCAGCGCTCC AAGTGGCTCC TGGACAGGCC ATGGCCCATC CATGGAGGAT
    TCCTCTGCTG TCCTCATGGA AAGCCTAAAG TCTATCTGGT CCAGTTCCAT GATGCATCCT
    GGACCTTCCG CTTTGGAGCA GCTGTTAATG CAGCAGAAGC AGAAACAGCA GCGGGGACAA
    GGCGCCATGA ACCCTCCACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu65968
    Clone ID Related Accession (Same CDS sequence) XM_025285494.1
    Accession Version XM_025285494.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein SMG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT
    CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT
    AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG
    TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT
    GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCATCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA
    CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC
    ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC GACAGGCCGG
    AATCTAAGCA ACAGCTGTGA CACGGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATT
    AAGCCCCGAG AAGTGAACAG AGACCAAGGC AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAGTAGC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG
    GCAGTGCAGG TAAAATCTCA GACAGAGTTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA
    ACACCTGTAA CTCAAACCCC AAGTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGGTTCC CGCCCCCAAC ATATGTTATC
    CCCCCTCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCACCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCCG GACCAGGGCC AGTGACCCAG
    GGACCTCAGC AGCCTCAGCC ACCTTCCCAG CAGCCCCTCC CACCTCTGCC AGCTCCGCCA
    GCAGCACAGA CTGCAGGCCA GCTGCAGGTT CAGGCTCTAG CTCAGCAGCA ACAGTCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGT CCTCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATCATGC CTGTGAAACA GCCCTACTAC CTTCAAACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCGT TGCAACCTCC TGTAAGGCAG CAGCAGCCTC TCGAGAAAAC AATGAAGCCT
    TTCCCCATGG AGCCATATAA CCATAACCCC TCAGAAGTCA AGATCCCAGA GTTCTACTGG
    GATTCTGCCT ACAGCGCTGC TGATAACAGA GCTGTGATGG CGCAGCAAGC CAGCGTGGAC
    CGCAGGGGCA GACGGTCCCC GGGAGTCTTC CGTCCAGAGC AGGACCCTGT GCCCAGGATG
    CCATTTGAGG ATCCCAAAGG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT
    GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCAGCTCTG
    CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTCTTTA AATCCTTACT GGAGAAGCCC
    TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC CCTCAATCAG
    GAACGATACC CAAATAACAG TGTGTTCAAT GAGGTATACG GGAAGAACCT GACAACCAGC
    TCCAAAGCAG AACTTAATCC CTCAATGGCT CCCCAGGAAA CATCATTGTA CTCGCTTTTT
    GAAGGGACTC CATGGTCTCC ATCTCTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC
    CAGTCTCCTC ATTCCTCCAA CCCAAGCAGC CTGCCAAGCT CCCCTCCAAC ACACAACCAC
    AATTCTGTCC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGGAGA
    ACTGCAGACC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT CGATTATCTC
    TCAGCAACAT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCGCTCCAAG TGGCTCCTGG
    ACAGGCCATG GCCCATCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT
    ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCCGCTT TGGAGCAGCT GTTAATGCAG
    CAGAAGCAGA AACAGCAGCG GGGACAAGGC GCCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025141279.1
    CDS142..3792
    Translation

    Target ORF information:

    RefSeq Version XM_025285494.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025285494.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT
    CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT
    AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG
    TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT
    GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCATCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA
    CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC
    ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC GACAGGCCGG
    AATCTAAGCA ACAGCTGTGA CACGGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATT
    AAGCCCCGAG AAGTGAACAG AGACCAAGGC AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAGTAGC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG
    GCAGTGCAGG TAAAATCTCA GACAGAGTTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA
    ACACCTGTAA CTCAAACCCC AAGTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGGTTCC CGCCCCCAAC ATATGTTATC
    CCCCCTCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCACCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCCG GACCAGGGCC AGTGACCCAG
    GGACCTCAGC AGCCTCAGCC ACCTTCCCAG CAGCCCCTCC CACCTCTGCC AGCTCCGCCA
    GCAGCACAGA CTGCAGGCCA GCTGCAGGTT CAGGCTCTAG CTCAGCAGCA ACAGTCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGT CCTCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATCATGC CTGTGAAACA GCCCTACTAC CTTCAAACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCGT TGCAACCTCC TGTAAGGCAG CAGCAGCCTC TCGAGAAAAC AATGAAGCCT
    TTCCCCATGG AGCCATATAA CCATAACCCC TCAGAAGTCA AGATCCCAGA GTTCTACTGG
    GATTCTGCCT ACAGCGCTGC TGATAACAGA GCTGTGATGG CGCAGCAAGC CAGCGTGGAC
    CGCAGGGGCA GACGGTCCCC GGGAGTCTTC CGTCCAGAGC AGGACCCTGT GCCCAGGATG
    CCATTTGAGG ATCCCAAAGG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT
    GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCAGCTCTG
    CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTCTTTA AATCCTTACT GGAGAAGCCC
    TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC CCTCAATCAG
    GAACGATACC CAAATAACAG TGTGTTCAAT GAGGTATACG GGAAGAACCT GACAACCAGC
    TCCAAAGCAG AACTTAATCC CTCAATGGCT CCCCAGGAAA CATCATTGTA CTCGCTTTTT
    GAAGGGACTC CATGGTCTCC ATCTCTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC
    CAGTCTCCTC ATTCCTCCAA CCCAAGCAGC CTGCCAAGCT CCCCTCCAAC ACACAACCAC
    AATTCTGTCC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGGAGA
    ACTGCAGACC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT CGATTATCTC
    TCAGCAACAT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCGCTCCAAG TGGCTCCTGG
    ACAGGCCATG GCCCATCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT
    ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCCGCTT TGGAGCAGCT GTTAATGCAG
    CAGAAGCAGA AACAGCAGCG GGGACAAGGC GCCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu65969
    Clone ID Related Accession (Same CDS sequence) XM_006048642.2
    Accession Version XM_006048642.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein SMG7 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006048642.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC CGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TTGTTACTGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAGGCAAA GAATCGAGCA
    AACCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTGTTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAGTTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTTCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCGGCTCAG
    CTTGTCCCCT CTAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CTACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCTA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGGTG
    AAAACCAAAT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAGAA AGCTTTCAAC TCTCAGCAGT TAGTTCACGT CACTGTCATT
    AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAACG AAACGGAGCA GCATAGTTAT
    AGCCAAGATG AGCAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT CCAGAACAAG TCTCAGGAGG AATCCTACAA TGCCTATCCC
    CTACCTGCCG TCAAGGTGTC CATGGACTGG CTGAGACTCA GACCCAGGGT GTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCGTGGCTAA TTTCTCTTCT GAACAGTTTC
    CATCCCCATG AAGAAGATCT TTCAAGTACT AATGCAACAC CACTTCCAGA GGAGTTTGAG
    TTACAAGGAT TCTTGGCTTT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAT
    CAGGGTATTA CAGGAGACAA AGAGGGTCAG CAACGACAAA TACGACAGCA GCGTTTGATC
    TCTATAGGCA AATGGATTGC AGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAGTTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGACACAACC ATCATAGAGT CCCTGGCAGC TGACGGGAAC
    CCAGGACTGA AATCAGTGCT GTCGACAGGC CGGAATCTAA GCAACAGCTG TGACACGGGA
    GAGAAACCAG TGGTCACCTT CAAAGAGAAC ATTAAGCCCC GAGAAGTGAA CAGAGACCAA
    GGCAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAGT AGCTGTAACT
    AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACAG AAACCAAGAA ATGCACCTTA
    GAAAAGTTAC AGGAAACAGG AAAGCAGAGT GTGGCAGTGC AGGTAAAATC TCAGACAGAG
    TTAAGAAAGA CTCCAGTGTC TGAAGCCAGG AAAACACCTG TAACTCAAAC CCCAAGTCAA
    GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC
    CGGCCAGGGT TCCCGCCCCC AACATATGTT ATCCCCCCTC CTGTGGCATT TTCTATGGGC
    TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT
    CACTCTCCAC CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG
    CAACGGCCCT CCGGACCAGG GCCAGTGACC CAGGGACCTC AGCAGCCTCA GCCACCTTCC
    CAGCAGCCCC TCCCACCTCT GCCAGCTCCG CCAGCAGCAC AGACTGCAGG CCAGCTGCAG
    GTTCAGGCTC TAGCTCAGCA GCAACAGTCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA
    AGTCCTCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG
    TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATCA TGCCTGTGAA ACAGCCCTAC
    TACCTTCAAA CCCAAGACCC CATAAAACTG TTTGAGCCGT CGTTGCAACC TCCTGTAAGG
    CAGCAGCAGC CTCTCGAGAA AACAATGAAG CCTTTCCCCA TGGAGCCATA TAACCATAAC
    CCCTCAGAAG TCAAGATCCC AGAGTTCTAC TGGGATTCTG CCTACAGCGC TGCTGATAAC
    AGAGCTGTGA TGGCGCAGCA AGCCAGCGTG GACCGCAGGG GCAGACGGTC CCCGGGAGTC
    TTCCGTCCAG AGCAGGACCC TGTGCCCAGG ATGCCATTTG AGGATCCCAA AGGCTCCCCT
    CTGCTTCCTC CGGACCTGTT AAAGAGTCTG GCTGCCTTGG AGGAAGAGGA AGAGCTGATT
    TTTTCTAACC CTCCTGATCT TTACCCAGCT CTGCTGGGGC CTCTCGCCTC TCTTCCTGGA
    CGAAGCCTCT TTAAATCCTT ACTGGAGAAG CCCTCAGAGC TCATGTCACA TTCATCCTCT
    TTCCTGTCCC TCACCGGATT CTCCCTCAAT CAGGAACGAT ACCCAAATAA CAGTGTGTTC
    AATGAGGTAT ACGGGAAGAA CCTGACAACC AGCTCCAAAG CAGAACTTAA TCCCTCAATG
    GCTCCCCAGG AAACATCATT GTACTCGCTT TTTGAAGGGA CTCCATGGTC TCCATCTCTT
    CCTGCCAGTT CAGATCATTC AACACCAGCC AGCCAGTCTC CTCATTCCTC CAACCCAAGC
    AGCCTGCCAA GCTCCCCTCC AACACACAAC CACAATTCTG TCCCATTCTC CAATTTTGGA
    CCCATTGGGA CTCCAGATAA CAGGGATAGG AGAACTGCAG ACCGGTGGAA AACTGATAAG
    CCAGCCATGG GTGGGTTTGG CATCGATTAT CTCTCAGCAA CATCATCCTC TGAGAGCAGT
    TGGCATCAGG CCAGCGCTCC AAGTGGCTCC TGGACAGGCC ATGGCCCATC CATGGAGGAT
    TCCTCTGCTG TCCTCATGGA AAGCCTAAAG TCTATCTGGT CCAGTTCCAT GATGCATCCT
    GGACCTTCCG CTTTGGAGCA GCTGTTAATG CAGCAGAAGC AGAAACAGCA GCGGGGACAA
    GGCGCCATGA ACCCTCCACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006048704.2
    CDS98..3661
    Translation

    Target ORF information:

    RefSeq Version XM_006048642.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006048642.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCCTGC AGAGCGCGCA GTATCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC CGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TTGTTACTGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCATG CCTTTAAGAA TCAGATCACA ACACTACAAG GCCAGGCAAA GAATCGAGCA
    AACCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTGTTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAGTTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTTCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCGGCTCAG
    CTTGTCCCCT CTAATGGTCA GCCTTACAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CTACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCTA CTAATCTACA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG GGATGAGGTG
    AAAACCAAAT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAGTTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAGAA AGCTTTCAAC TCTCAGCAGT TAGTTCACGT CACTGTCATT
    AACCTGTTTC AACTTCATCA TCTTCGTGAC TTTAGCAACG AAACGGAGCA GCATAGTTAT
    AGCCAAGATG AGCAGCTGTG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT CCAGAACAAG TCTCAGGAGG AATCCTACAA TGCCTATCCC
    CTACCTGCCG TCAAGGTGTC CATGGACTGG CTGAGACTCA GACCCAGGGT GTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCGTGGCTAA TTTCTCTTCT GAACAGTTTC
    CATCCCCATG AAGAAGATCT TTCAAGTACT AATGCAACAC CACTTCCAGA GGAGTTTGAG
    TTACAAGGAT TCTTGGCTTT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAT
    CAGGGTATTA CAGGAGACAA AGAGGGTCAG CAACGACAAA TACGACAGCA GCGTTTGATC
    TCTATAGGCA AATGGATTGC AGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAGTTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGACACAACC ATCATAGAGT CCCTGGCAGC TGACGGGAAC
    CCAGGACTGA AATCAGTGCT GTCGACAGGC CGGAATCTAA GCAACAGCTG TGACACGGGA
    GAGAAACCAG TGGTCACCTT CAAAGAGAAC ATTAAGCCCC GAGAAGTGAA CAGAGACCAA
    GGCAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAGT AGCTGTAACT
    AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACAG AAACCAAGAA ATGCACCTTA
    GAAAAGTTAC AGGAAACAGG AAAGCAGAGT GTGGCAGTGC AGGTAAAATC TCAGACAGAG
    TTAAGAAAGA CTCCAGTGTC TGAAGCCAGG AAAACACCTG TAACTCAAAC CCCAAGTCAA
    GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC
    CGGCCAGGGT TCCCGCCCCC AACATATGTT ATCCCCCCTC CTGTGGCATT TTCTATGGGC
    TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT
    CACTCTCCAC CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG
    CAACGGCCCT CCGGACCAGG GCCAGTGACC CAGGGACCTC AGCAGCCTCA GCCACCTTCC
    CAGCAGCCCC TCCCACCTCT GCCAGCTCCG CCAGCAGCAC AGACTGCAGG CCAGCTGCAG
    GTTCAGGCTC TAGCTCAGCA GCAACAGTCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA
    AGTCCTCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG
    TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATCA TGCCTGTGAA ACAGCCCTAC
    TACCTTCAAA CCCAAGACCC CATAAAACTG TTTGAGCCGT CGTTGCAACC TCCTGTAAGG
    CAGCAGCAGC CTCTCGAGAA AACAATGAAG CCTTTCCCCA TGGAGCCATA TAACCATAAC
    CCCTCAGAAG TCAAGATCCC AGAGTTCTAC TGGGATTCTG CCTACAGCGC TGCTGATAAC
    AGAGCTGTGA TGGCGCAGCA AGCCAGCGTG GACCGCAGGG GCAGACGGTC CCCGGGAGTC
    TTCCGTCCAG AGCAGGACCC TGTGCCCAGG ATGCCATTTG AGGATCCCAA AGGCTCCCCT
    CTGCTTCCTC CGGACCTGTT AAAGAGTCTG GCTGCCTTGG AGGAAGAGGA AGAGCTGATT
    TTTTCTAACC CTCCTGATCT TTACCCAGCT CTGCTGGGGC CTCTCGCCTC TCTTCCTGGA
    CGAAGCCTCT TTAAATCCTT ACTGGAGAAG CCCTCAGAGC TCATGTCACA TTCATCCTCT
    TTCCTGTCCC TCACCGGATT CTCCCTCAAT CAGGAACGAT ACCCAAATAA CAGTGTGTTC
    AATGAGGTAT ACGGGAAGAA CCTGACAACC AGCTCCAAAG CAGAACTTAA TCCCTCAATG
    GCTCCCCAGG AAACATCATT GTACTCGCTT TTTGAAGGGA CTCCATGGTC TCCATCTCTT
    CCTGCCAGTT CAGATCATTC AACACCAGCC AGCCAGTCTC CTCATTCCTC CAACCCAAGC
    AGCCTGCCAA GCTCCCCTCC AACACACAAC CACAATTCTG TCCCATTCTC CAATTTTGGA
    CCCATTGGGA CTCCAGATAA CAGGGATAGG AGAACTGCAG ACCGGTGGAA AACTGATAAG
    CCAGCCATGG GTGGGTTTGG CATCGATTAT CTCTCAGCAA CATCATCCTC TGAGAGCAGT
    TGGCATCAGG CCAGCGCTCC AAGTGGCTCC TGGACAGGCC ATGGCCCATC CATGGAGGAT
    TCCTCTGCTG TCCTCATGGA AAGCCTAAAG TCTATCTGGT CCAGTTCCAT GATGCATCCT
    GGACCTTCCG CTTTGGAGCA GCTGTTAATG CAGCAGAAGC AGAAACAGCA GCGGGGACAA
    GGCGCCATGA ACCCTCCACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu65970
    Clone ID Related Accession (Same CDS sequence) XM_025285495.1
    Accession Version XM_025285495.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein SMG7 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT
    CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT
    AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG
    TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT
    GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCATCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA
    CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC
    ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC GACAGGCCGG
    AATCTAAGCA ACAGCTGTGA CACGGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATT
    AAGCCCCGAG AAGTGAACAG AGACCAAGGC AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAGTAGC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG
    GCAGTGCAGG TAAAATCTCA GACAGAGTTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA
    ACACCTGTAA CTCAAACCCC AAGTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGAACCT TTCTTCAGCC TACAGCTCAC
    TCTCCACCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCCG GACCAGGGCC AGTGACCCAG GGACCTCAGC AGCCTCAGCC ACCTTCCCAG
    CAGCCCCTCC CACCTCTGCC AGCTCCGCCA GCAGCACAGA CTGCAGGCCA GCTGCAGGTT
    CAGGCTCTAG CTCAGCAGCA ACAGTCCCCT ACAAAAGCTG TGCCGGCTTT GGGGAAAAGT
    CCTCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG
    AATCCCCCTC AGGTTCAAGG CCCATTAGGG AAAATCATGC CTGTGAAACA GCCCTACTAC
    CTTCAAACCC AAGACCCCAT AAAACTGTTT GAGCCGTCGT TGCAACCTCC TGTAAGGCAG
    CAGCAGCCTC TCGAGAAAAC AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAACCCC
    TCAGAAGTCA AGATCCCAGA GTTCTACTGG GATTCTGCCT ACAGCGCTGC TGATAACAGA
    GCTGTGATGG CGCAGCAAGC CAGCGTGGAC CGCAGGGGCA GACGGTCCCC GGGAGTCTTC
    CGTCCAGAGC AGGACCCTGT GCCCAGGATG CCATTTGAGG ATCCCAAAGG CTCCCCTCTG
    CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATTTTT
    TCTAACCCTC CTGATCTTTA CCCAGCTCTG CTGGGGCCTC TCGCCTCTCT TCCTGGACGA
    AGCCTCTTTA AATCCTTACT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC
    CTGTCCCTCA CCGGATTCTC CCTCAATCAG GAACGATACC CAAATAACAG TGTGTTCAAT
    GAGGTATACG GGAAGAACCT GACAACCAGC TCCAAAGCAG AACTTAATCC CTCAATGGCT
    CCCCAGGAAA CATCATTGTA CTCGCTTTTT GAAGGGACTC CATGGTCTCC ATCTCTTCCT
    GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCCAA CCCAAGCAGC
    CTGCCAAGCT CCCCTCCAAC ACACAACCAC AATTCTGTCC CATTCTCCAA TTTTGGACCC
    ATTGGGACTC CAGATAACAG GGATAGGAGA ACTGCAGACC GGTGGAAAAC TGATAAGCCA
    GCCATGGGTG GGTTTGGCAT CGATTATCTC TCAGCAACAT CATCCTCTGA GAGCAGTTGG
    CATCAGGCCA GCGCTCCAAG TGGCTCCTGG ACAGGCCATG GCCCATCCAT GGAGGATTCC
    TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA
    CCTTCCGCTT TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGACAAGGC
    GCCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025141280.1
    CDS142..3702
    Translation

    Target ORF information:

    RefSeq Version XM_025285495.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025285495.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT
    CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT
    AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG
    TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT
    GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCATCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA
    CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC
    ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC GACAGGCCGG
    AATCTAAGCA ACAGCTGTGA CACGGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATT
    AAGCCCCGAG AAGTGAACAG AGACCAAGGC AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAGTAGC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG
    GCAGTGCAGG TAAAATCTCA GACAGAGTTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA
    ACACCTGTAA CTCAAACCCC AAGTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGAACCT TTCTTCAGCC TACAGCTCAC
    TCTCCACCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCCG GACCAGGGCC AGTGACCCAG GGACCTCAGC AGCCTCAGCC ACCTTCCCAG
    CAGCCCCTCC CACCTCTGCC AGCTCCGCCA GCAGCACAGA CTGCAGGCCA GCTGCAGGTT
    CAGGCTCTAG CTCAGCAGCA ACAGTCCCCT ACAAAAGCTG TGCCGGCTTT GGGGAAAAGT
    CCTCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG
    AATCCCCCTC AGGTTCAAGG CCCATTAGGG AAAATCATGC CTGTGAAACA GCCCTACTAC
    CTTCAAACCC AAGACCCCAT AAAACTGTTT GAGCCGTCGT TGCAACCTCC TGTAAGGCAG
    CAGCAGCCTC TCGAGAAAAC AATGAAGCCT TTCCCCATGG AGCCATATAA CCATAACCCC
    TCAGAAGTCA AGATCCCAGA GTTCTACTGG GATTCTGCCT ACAGCGCTGC TGATAACAGA
    GCTGTGATGG CGCAGCAAGC CAGCGTGGAC CGCAGGGGCA GACGGTCCCC GGGAGTCTTC
    CGTCCAGAGC AGGACCCTGT GCCCAGGATG CCATTTGAGG ATCCCAAAGG CTCCCCTCTG
    CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATTTTT
    TCTAACCCTC CTGATCTTTA CCCAGCTCTG CTGGGGCCTC TCGCCTCTCT TCCTGGACGA
    AGCCTCTTTA AATCCTTACT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC
    CTGTCCCTCA CCGGATTCTC CCTCAATCAG GAACGATACC CAAATAACAG TGTGTTCAAT
    GAGGTATACG GGAAGAACCT GACAACCAGC TCCAAAGCAG AACTTAATCC CTCAATGGCT
    CCCCAGGAAA CATCATTGTA CTCGCTTTTT GAAGGGACTC CATGGTCTCC ATCTCTTCCT
    GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCCAA CCCAAGCAGC
    CTGCCAAGCT CCCCTCCAAC ACACAACCAC AATTCTGTCC CATTCTCCAA TTTTGGACCC
    ATTGGGACTC CAGATAACAG GGATAGGAGA ACTGCAGACC GGTGGAAAAC TGATAAGCCA
    GCCATGGGTG GGTTTGGCAT CGATTATCTC TCAGCAACAT CATCCTCTGA GAGCAGTTGG
    CATCAGGCCA GCGCTCCAAG TGGCTCCTGG ACAGGCCATG GCCCATCCAT GGAGGATTCC
    TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA
    CCTTCCGCTT TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGACAAGGC
    GCCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu65971
    Clone ID Related Accession (Same CDS sequence) XM_025285496.1
    Accession Version XM_025285496.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3513bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein SMG7 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT
    CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT
    AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG
    TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT
    GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCATCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA
    CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC
    ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC GACAGGCCGG
    AATCTAAGCA ACAGCTGTGA CACGGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATT
    AAGCCCCGAG AAGTGAACAG AGACCAAGGC AGAAGTTTTC CTCCCAAAGA GGTAAAATCT
    CAGACAGAGT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACACCTGT AACTCAAACC
    CCAAGTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCC GGCCAGGGTT CCCGCCCCCA ACATATGTTA TCCCCCCTCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCACC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC CGGACCAGGG CCAGTGACCC AGGGACCTCA GCAGCCTCAG
    CCACCTTCCC AGCAGCCCCT CCCACCTCTG CCAGCTCCGC CAGCAGCACA GACTGCAGGC
    CAGCTGCAGG TTCAGGCTCT AGCTCAGCAG CAACAGTCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GTCCTCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATCAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAAAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC GTTGCAACCT
    CCTGTAAGGC AGCAGCAGCC TCTCGAGAAA ACAATGAAGC CTTTCCCCAT GGAGCCATAT
    AACCATAACC CCTCAGAAGT CAAGATCCCA GAGTTCTACT GGGATTCTGC CTACAGCGCT
    GCTGATAACA GAGCTGTGAT GGCGCAGCAA GCCAGCGTGG ACCGCAGGGG CAGACGGTCC
    CCGGGAGTCT TCCGTCCAGA GCAGGACCCT GTGCCCAGGA TGCCATTTGA GGATCCCAAA
    GGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA
    GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCAGCTC TGCTGGGGCC TCTCGCCTCT
    CTTCCTGGAC GAAGCCTCTT TAAATCCTTA CTGGAGAAGC CCTCAGAGCT CATGTCACAT
    TCATCCTCTT TCCTGTCCCT CACCGGATTC TCCCTCAATC AGGAACGATA CCCAAATAAC
    AGTGTGTTCA ATGAGGTATA CGGGAAGAAC CTGACAACCA GCTCCAAAGC AGAACTTAAT
    CCCTCAATGG CTCCCCAGGA AACATCATTG TACTCGCTTT TTGAAGGGAC TCCATGGTCT
    CCATCTCTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCC
    AACCCAAGCA GCCTGCCAAG CTCCCCTCCA ACACACAACC ACAATTCTGT CCCATTCTCC
    AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGGA GAACTGCAGA CCGGTGGAAA
    ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATCGATTATC TCTCAGCAAC ATCATCCTCT
    GAGAGCAGTT GGCATCAGGC CAGCGCTCCA AGTGGCTCCT GGACAGGCCA TGGCCCATCC
    ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG
    ATGCATCCTG GACCTTCCGC TTTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAG
    CGGGGACAAG GCGCCATGAA CCCTCCACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025141281.1
    CDS142..3654
    Translation

    Target ORF information:

    RefSeq Version XM_025285496.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025285496.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT
    CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT
    AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG
    TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT
    GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCATCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA
    CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC
    ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC GACAGGCCGG
    AATCTAAGCA ACAGCTGTGA CACGGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATT
    AAGCCCCGAG AAGTGAACAG AGACCAAGGC AGAAGTTTTC CTCCCAAAGA GGTAAAATCT
    CAGACAGAGT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACACCTGT AACTCAAACC
    CCAAGTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCC GGCCAGGGTT CCCGCCCCCA ACATATGTTA TCCCCCCTCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCACC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC CGGACCAGGG CCAGTGACCC AGGGACCTCA GCAGCCTCAG
    CCACCTTCCC AGCAGCCCCT CCCACCTCTG CCAGCTCCGC CAGCAGCACA GACTGCAGGC
    CAGCTGCAGG TTCAGGCTCT AGCTCAGCAG CAACAGTCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GTCCTCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATCAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAAAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC GTTGCAACCT
    CCTGTAAGGC AGCAGCAGCC TCTCGAGAAA ACAATGAAGC CTTTCCCCAT GGAGCCATAT
    AACCATAACC CCTCAGAAGT CAAGATCCCA GAGTTCTACT GGGATTCTGC CTACAGCGCT
    GCTGATAACA GAGCTGTGAT GGCGCAGCAA GCCAGCGTGG ACCGCAGGGG CAGACGGTCC
    CCGGGAGTCT TCCGTCCAGA GCAGGACCCT GTGCCCAGGA TGCCATTTGA GGATCCCAAA
    GGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA
    GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCAGCTC TGCTGGGGCC TCTCGCCTCT
    CTTCCTGGAC GAAGCCTCTT TAAATCCTTA CTGGAGAAGC CCTCAGAGCT CATGTCACAT
    TCATCCTCTT TCCTGTCCCT CACCGGATTC TCCCTCAATC AGGAACGATA CCCAAATAAC
    AGTGTGTTCA ATGAGGTATA CGGGAAGAAC CTGACAACCA GCTCCAAAGC AGAACTTAAT
    CCCTCAATGG CTCCCCAGGA AACATCATTG TACTCGCTTT TTGAAGGGAC TCCATGGTCT
    CCATCTCTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCC
    AACCCAAGCA GCCTGCCAAG CTCCCCTCCA ACACACAACC ACAATTCTGT CCCATTCTCC
    AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGGA GAACTGCAGA CCGGTGGAAA
    ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATCGATTATC TCTCAGCAAC ATCATCCTCT
    GAGAGCAGTT GGCATCAGGC CAGCGCTCCA AGTGGCTCCT GGACAGGCCA TGGCCCATCC
    ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG
    ATGCATCCTG GACCTTCCGC TTTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAG
    CGGGGACAAG GCGCCATGAA CCCTCCACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu65972
    Clone ID Related Accession (Same CDS sequence) XM_025285498.1 , XM_025285497.1
    Accession Version XM_025285498.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3510bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein SMG7 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACAGATT CTAAGCTGGG TCCAGCCGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
    CTGTACCAGA AAATGCTTGT TACTGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG
    GATCTCTGGA ATCATGCCTT TAAGAATCAG ATCACAACAC TACAAGGCCA GGCAAAGAAT
    CGAGCAAACC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT
    AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TGTTTAATGT AGATTTACCA
    TGCCGTGTGA AGTCTTCCCA GTTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC
    ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTTCACCTT
    GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCG
    GCTCAGCTTG TCCCCTCTAA TGGTCAGCCT TACAATCAGT TGGCTATCTT AGCTTCTTCC
    AAAGGAGACC ATCTGACTAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT
    TTCCCAGCTG CCTCTACTAA TCTACAAAAA GCACTTTCTA AAGCACTGGA AAGCCGGGAT
    GAGGTGAAAA CCAAATGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT
    CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA GTTGGAAGAA
    CAGTTTAAGA GGCTGCTATT CCAGAAAGCT TTCAACTCTC AGCAGTTAGT TCACGTCACT
    GTCATTAACC TGTTTCAACT TCATCATCTT CGTGACTTTA GCAACGAAAC GGAGCAGCAT
    AGTTATAGCC AAGATGAGCA GCTGTGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT
    CTTGGCATCC TGTGCAAGTG TCCTCTCCAG AACAAGTCTC AGGAGGAATC CTACAATGCC
    TATCCCCTAC CTGCCGTCAA GGTGTCCATG GACTGGCTGA GACTCAGACC CAGGGTGTTT
    CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCGT GGCTAATTTC TCTTCTGAAC
    AGTTTCCATC CCCATGAAGA AGATCTTTCA AGTACTAATG CAACACCACT TCCAGAGGAG
    TTTGAGTTAC AAGGATTCTT GGCTTTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA
    GGTCATCAGG GTATTACAGG AGACAAAGAG GGTCAGCAAC GACAAATACG ACAGCAGCGT
    TTGATCTCTA TAGGCAAATG GATTGCAGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT
    GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAGT TAATACTGGA AGACCCCAGT
    GAAGCCAAAG AGAACCTCAT TCTGCAAGAC ACAACCATCA TAGAGTCCCT GGCAGCTGAC
    GGGAACCCAG GACTGAAATC AGTGCTGTCG ACAGGCCGGA ATCTAAGCAA CAGCTGTGAC
    ACGGGAGAGA AACCAGTGGT CACCTTCAAA GAGAACATTA AGCCCCGAGA AGTGAACAGA
    GACCAAGGCA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAGTAGCT
    GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACAGAAAC CAAGAAATGC
    ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAGTGTGG CAGTGCAGGT AAAATCTCAG
    ACAGAGTTAA GAAAGACTCC AGTGTCTGAA GCCAGGAAAA CACCTGTAAC TCAAACCCCA
    AGTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT
    CCCAGCCGGC CAGGGTTCCC GCCCCCAACA TATGTTATCC CCCCTCCTGT GGCATTTTCT
    ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT
    ACAGCTCACT CTCCACCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC
    AGCCAGCAAC GGCCCTCCGG ACCAGGGCCA GTGACCCAGG GACCTCAGCA GCCTCAGCCA
    CCTTCCCAGC AGCCCCTCCC ACCTCTGCCA GCTCCGCCAG CAGCACAGAC TGCAGGCCAG
    CTGCAGGTTC AGGCTCTAGC TCAGCAGCAA CAGTCCCCTA CAAAAGCTGT GCCGGCTTTG
    GGGAAAAGTC CTCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATCATGCC TGTGAAACAG
    CCCTACTACC TTCAAACCCA AGACCCCATA AAACTGTTTG AGCCGTCGTT GCAACCTCCT
    GTAAGGCAGC AGCAGCCTCT CGAGAAAACA ATGAAGCCTT TCCCCATGGA GCCATATAAC
    CATAACCCCT CAGAAGTCAA GATCCCAGAG TTCTACTGGG ATTCTGCCTA CAGCGCTGCT
    GATAACAGAG CTGTGATGGC GCAGCAAGCC AGCGTGGACC GCAGGGGCAG ACGGTCCCCG
    GGAGTCTTCC GTCCAGAGCA GGACCCTGTG CCCAGGATGC CATTTGAGGA TCCCAAAGGC
    TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG
    CTGATTTTTT CTAACCCTCC TGATCTTTAC CCAGCTCTGC TGGGGCCTCT CGCCTCTCTT
    CCTGGACGAA GCCTCTTTAA ATCCTTACTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA
    TCCTCTTTCC TGTCCCTCAC CGGATTCTCC CTCAATCAGG AACGATACCC AAATAACAGT
    GTGTTCAATG AGGTATACGG GAAGAACCTG ACAACCAGCT CCAAAGCAGA ACTTAATCCC
    TCAATGGCTC CCCAGGAAAC ATCATTGTAC TCGCTTTTTG AAGGGACTCC ATGGTCTCCA
    TCTCTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCCAAC
    CCAAGCAGCC TGCCAAGCTC CCCTCCAACA CACAACCACA ATTCTGTCCC ATTCTCCAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGGAGAA CTGCAGACCG GTGGAAAACT
    GATAAGCCAG CCATGGGTGG GTTTGGCATC GATTATCTCT CAGCAACATC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CGCTCCAAGT GGCTCCTGGA CAGGCCATGG CCCATCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCCGCTTT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAGCGG
    GGACAAGGCG CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025141283.1
    CDS219..3728
    Translation

    Target ORF information:

    RefSeq Version XM_025285498.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025285498.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACAGATT CTAAGCTGGG TCCAGCCGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
    CTGTACCAGA AAATGCTTGT TACTGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG
    GATCTCTGGA ATCATGCCTT TAAGAATCAG ATCACAACAC TACAAGGCCA GGCAAAGAAT
    CGAGCAAACC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT
    AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TGTTTAATGT AGATTTACCA
    TGCCGTGTGA AGTCTTCCCA GTTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC
    ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTTCACCTT
    GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCG
    GCTCAGCTTG TCCCCTCTAA TGGTCAGCCT TACAATCAGT TGGCTATCTT AGCTTCTTCC
    AAAGGAGACC ATCTGACTAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT
    TTCCCAGCTG CCTCTACTAA TCTACAAAAA GCACTTTCTA AAGCACTGGA AAGCCGGGAT
    GAGGTGAAAA CCAAATGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT
    CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA GTTGGAAGAA
    CAGTTTAAGA GGCTGCTATT CCAGAAAGCT TTCAACTCTC AGCAGTTAGT TCACGTCACT
    GTCATTAACC TGTTTCAACT TCATCATCTT CGTGACTTTA GCAACGAAAC GGAGCAGCAT
    AGTTATAGCC AAGATGAGCA GCTGTGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT
    CTTGGCATCC TGTGCAAGTG TCCTCTCCAG AACAAGTCTC AGGAGGAATC CTACAATGCC
    TATCCCCTAC CTGCCGTCAA GGTGTCCATG GACTGGCTGA GACTCAGACC CAGGGTGTTT
    CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCGT GGCTAATTTC TCTTCTGAAC
    AGTTTCCATC CCCATGAAGA AGATCTTTCA AGTACTAATG CAACACCACT TCCAGAGGAG
    TTTGAGTTAC AAGGATTCTT GGCTTTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA
    GGTCATCAGG GTATTACAGG AGACAAAGAG GGTCAGCAAC GACAAATACG ACAGCAGCGT
    TTGATCTCTA TAGGCAAATG GATTGCAGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT
    GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAGT TAATACTGGA AGACCCCAGT
    GAAGCCAAAG AGAACCTCAT TCTGCAAGAC ACAACCATCA TAGAGTCCCT GGCAGCTGAC
    GGGAACCCAG GACTGAAATC AGTGCTGTCG ACAGGCCGGA ATCTAAGCAA CAGCTGTGAC
    ACGGGAGAGA AACCAGTGGT CACCTTCAAA GAGAACATTA AGCCCCGAGA AGTGAACAGA
    GACCAAGGCA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAGTAGCT
    GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACAGAAAC CAAGAAATGC
    ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAGTGTGG CAGTGCAGGT AAAATCTCAG
    ACAGAGTTAA GAAAGACTCC AGTGTCTGAA GCCAGGAAAA CACCTGTAAC TCAAACCCCA
    AGTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT
    CCCAGCCGGC CAGGGTTCCC GCCCCCAACA TATGTTATCC CCCCTCCTGT GGCATTTTCT
    ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT
    ACAGCTCACT CTCCACCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC
    AGCCAGCAAC GGCCCTCCGG ACCAGGGCCA GTGACCCAGG GACCTCAGCA GCCTCAGCCA
    CCTTCCCAGC AGCCCCTCCC ACCTCTGCCA GCTCCGCCAG CAGCACAGAC TGCAGGCCAG
    CTGCAGGTTC AGGCTCTAGC TCAGCAGCAA CAGTCCCCTA CAAAAGCTGT GCCGGCTTTG
    GGGAAAAGTC CTCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATCATGCC TGTGAAACAG
    CCCTACTACC TTCAAACCCA AGACCCCATA AAACTGTTTG AGCCGTCGTT GCAACCTCCT
    GTAAGGCAGC AGCAGCCTCT CGAGAAAACA ATGAAGCCTT TCCCCATGGA GCCATATAAC
    CATAACCCCT CAGAAGTCAA GATCCCAGAG TTCTACTGGG ATTCTGCCTA CAGCGCTGCT
    GATAACAGAG CTGTGATGGC GCAGCAAGCC AGCGTGGACC GCAGGGGCAG ACGGTCCCCG
    GGAGTCTTCC GTCCAGAGCA GGACCCTGTG CCCAGGATGC CATTTGAGGA TCCCAAAGGC
    TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG
    CTGATTTTTT CTAACCCTCC TGATCTTTAC CCAGCTCTGC TGGGGCCTCT CGCCTCTCTT
    CCTGGACGAA GCCTCTTTAA ATCCTTACTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA
    TCCTCTTTCC TGTCCCTCAC CGGATTCTCC CTCAATCAGG AACGATACCC AAATAACAGT
    GTGTTCAATG AGGTATACGG GAAGAACCTG ACAACCAGCT CCAAAGCAGA ACTTAATCCC
    TCAATGGCTC CCCAGGAAAC ATCATTGTAC TCGCTTTTTG AAGGGACTCC ATGGTCTCCA
    TCTCTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCCAAC
    CCAAGCAGCC TGCCAAGCTC CCCTCCAACA CACAACCACA ATTCTGTCCC ATTCTCCAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGGAGAA CTGCAGACCG GTGGAAAACT
    GATAAGCCAG CCATGGGTGG GTTTGGCATC GATTATCTCT CAGCAACATC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CGCTCCAAGT GGCTCCTGGA CAGGCCATGG CCCATCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCCGCTTT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAGCGG
    GGACAAGGCG CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu65972
    Clone ID Related Accession (Same CDS sequence) XM_025285498.1 , XM_025285497.1
    Accession Version XM_025285497.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3510bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein SMG7 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACAGATT CTAAGCTGGG TCCAGCCGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
    CTGTACCAGA AAATGCTTGT TACTGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG
    GATCTCTGGA ATCATGCCTT TAAGAATCAG ATCACAACAC TACAAGGCCA GGCAAAGAAT
    CGAGCAAACC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT
    AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TGTTTAATGT AGATTTACCA
    TGCCGTGTGA AGTCTTCCCA GTTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC
    ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTTCACCTT
    GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCG
    GCTCAGCTTG TCCCCTCTAA TGGTCAGCCT TACAATCAGT TGGCTATCTT AGCTTCTTCC
    AAAGGAGACC ATCTGACTAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT
    TTCCCAGCTG CCTCTACTAA TCTACAAAAA GCACTTTCTA AAGCACTGGA AAGCCGGGAT
    GAGGTGAAAA CCAAATGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT
    CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA GTTGGAAGAA
    CAGTTTAAGA GGCTGCTATT CCAGAAAGCT TTCAACTCTC AGCAGTTAGT TCACGTCACT
    GTCATTAACC TGTTTCAACT TCATCATCTT CGTGACTTTA GCAACGAAAC GGAGCAGCAT
    AGTTATAGCC AAGATGAGCA GCTGTGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT
    CTTGGCATCC TGTGCAAGTG TCCTCTCCAG AACAAGTCTC AGGAGGAATC CTACAATGCC
    TATCCCCTAC CTGCCGTCAA GGTGTCCATG GACTGGCTGA GACTCAGACC CAGGGTGTTT
    CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCGT GGCTAATTTC TCTTCTGAAC
    AGTTTCCATC CCCATGAAGA AGATCTTTCA AGTACTAATG CAACACCACT TCCAGAGGAG
    TTTGAGTTAC AAGGATTCTT GGCTTTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA
    GGTCATCAGG GTATTACAGG AGACAAAGAG GGTCAGCAAC GACAAATACG ACAGCAGCGT
    TTGATCTCTA TAGGCAAATG GATTGCAGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT
    GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAGT TAATACTGGA AGACCCCAGT
    GAAGCCAAAG AGAACCTCAT TCTGCAAGAC ACAACCATCA TAGAGTCCCT GGCAGCTGAC
    GGGAACCCAG GACTGAAATC AGTGCTGTCG ACAGGCCGGA ATCTAAGCAA CAGCTGTGAC
    ACGGGAGAGA AACCAGTGGT CACCTTCAAA GAGAACATTA AGCCCCGAGA AGTGAACAGA
    GACCAAGGCA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAGTAGCT
    GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACAGAAAC CAAGAAATGC
    ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAGTGTGG CAGTGCAGGT AAAATCTCAG
    ACAGAGTTAA GAAAGACTCC AGTGTCTGAA GCCAGGAAAA CACCTGTAAC TCAAACCCCA
    AGTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT
    CCCAGCCGGC CAGGGTTCCC GCCCCCAACA TATGTTATCC CCCCTCCTGT GGCATTTTCT
    ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT
    ACAGCTCACT CTCCACCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC
    AGCCAGCAAC GGCCCTCCGG ACCAGGGCCA GTGACCCAGG GACCTCAGCA GCCTCAGCCA
    CCTTCCCAGC AGCCCCTCCC ACCTCTGCCA GCTCCGCCAG CAGCACAGAC TGCAGGCCAG
    CTGCAGGTTC AGGCTCTAGC TCAGCAGCAA CAGTCCCCTA CAAAAGCTGT GCCGGCTTTG
    GGGAAAAGTC CTCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATCATGCC TGTGAAACAG
    CCCTACTACC TTCAAACCCA AGACCCCATA AAACTGTTTG AGCCGTCGTT GCAACCTCCT
    GTAAGGCAGC AGCAGCCTCT CGAGAAAACA ATGAAGCCTT TCCCCATGGA GCCATATAAC
    CATAACCCCT CAGAAGTCAA GATCCCAGAG TTCTACTGGG ATTCTGCCTA CAGCGCTGCT
    GATAACAGAG CTGTGATGGC GCAGCAAGCC AGCGTGGACC GCAGGGGCAG ACGGTCCCCG
    GGAGTCTTCC GTCCAGAGCA GGACCCTGTG CCCAGGATGC CATTTGAGGA TCCCAAAGGC
    TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG
    CTGATTTTTT CTAACCCTCC TGATCTTTAC CCAGCTCTGC TGGGGCCTCT CGCCTCTCTT
    CCTGGACGAA GCCTCTTTAA ATCCTTACTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA
    TCCTCTTTCC TGTCCCTCAC CGGATTCTCC CTCAATCAGG AACGATACCC AAATAACAGT
    GTGTTCAATG AGGTATACGG GAAGAACCTG ACAACCAGCT CCAAAGCAGA ACTTAATCCC
    TCAATGGCTC CCCAGGAAAC ATCATTGTAC TCGCTTTTTG AAGGGACTCC ATGGTCTCCA
    TCTCTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCCAAC
    CCAAGCAGCC TGCCAAGCTC CCCTCCAACA CACAACCACA ATTCTGTCCC ATTCTCCAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGGAGAA CTGCAGACCG GTGGAAAACT
    GATAAGCCAG CCATGGGTGG GTTTGGCATC GATTATCTCT CAGCAACATC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CGCTCCAAGT GGCTCCTGGA CAGGCCATGG CCCATCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCCGCTTT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAGCGG
    GGACAAGGCG CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025141282.1
    CDS202..3711
    Translation

    Target ORF information:

    RefSeq Version XM_025285497.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025285497.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACAGATT CTAAGCTGGG TCCAGCCGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
    CTGTACCAGA AAATGCTTGT TACTGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG
    GATCTCTGGA ATCATGCCTT TAAGAATCAG ATCACAACAC TACAAGGCCA GGCAAAGAAT
    CGAGCAAACC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT
    AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TGTTTAATGT AGATTTACCA
    TGCCGTGTGA AGTCTTCCCA GTTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC
    ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTTCACCTT
    GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCG
    GCTCAGCTTG TCCCCTCTAA TGGTCAGCCT TACAATCAGT TGGCTATCTT AGCTTCTTCC
    AAAGGAGACC ATCTGACTAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT
    TTCCCAGCTG CCTCTACTAA TCTACAAAAA GCACTTTCTA AAGCACTGGA AAGCCGGGAT
    GAGGTGAAAA CCAAATGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT
    CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA GTTGGAAGAA
    CAGTTTAAGA GGCTGCTATT CCAGAAAGCT TTCAACTCTC AGCAGTTAGT TCACGTCACT
    GTCATTAACC TGTTTCAACT TCATCATCTT CGTGACTTTA GCAACGAAAC GGAGCAGCAT
    AGTTATAGCC AAGATGAGCA GCTGTGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT
    CTTGGCATCC TGTGCAAGTG TCCTCTCCAG AACAAGTCTC AGGAGGAATC CTACAATGCC
    TATCCCCTAC CTGCCGTCAA GGTGTCCATG GACTGGCTGA GACTCAGACC CAGGGTGTTT
    CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCGT GGCTAATTTC TCTTCTGAAC
    AGTTTCCATC CCCATGAAGA AGATCTTTCA AGTACTAATG CAACACCACT TCCAGAGGAG
    TTTGAGTTAC AAGGATTCTT GGCTTTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA
    GGTCATCAGG GTATTACAGG AGACAAAGAG GGTCAGCAAC GACAAATACG ACAGCAGCGT
    TTGATCTCTA TAGGCAAATG GATTGCAGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT
    GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAGT TAATACTGGA AGACCCCAGT
    GAAGCCAAAG AGAACCTCAT TCTGCAAGAC ACAACCATCA TAGAGTCCCT GGCAGCTGAC
    GGGAACCCAG GACTGAAATC AGTGCTGTCG ACAGGCCGGA ATCTAAGCAA CAGCTGTGAC
    ACGGGAGAGA AACCAGTGGT CACCTTCAAA GAGAACATTA AGCCCCGAGA AGTGAACAGA
    GACCAAGGCA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAGTAGCT
    GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACAGAAAC CAAGAAATGC
    ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAGTGTGG CAGTGCAGGT AAAATCTCAG
    ACAGAGTTAA GAAAGACTCC AGTGTCTGAA GCCAGGAAAA CACCTGTAAC TCAAACCCCA
    AGTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT
    CCCAGCCGGC CAGGGTTCCC GCCCCCAACA TATGTTATCC CCCCTCCTGT GGCATTTTCT
    ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT
    ACAGCTCACT CTCCACCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC
    AGCCAGCAAC GGCCCTCCGG ACCAGGGCCA GTGACCCAGG GACCTCAGCA GCCTCAGCCA
    CCTTCCCAGC AGCCCCTCCC ACCTCTGCCA GCTCCGCCAG CAGCACAGAC TGCAGGCCAG
    CTGCAGGTTC AGGCTCTAGC TCAGCAGCAA CAGTCCCCTA CAAAAGCTGT GCCGGCTTTG
    GGGAAAAGTC CTCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATCATGCC TGTGAAACAG
    CCCTACTACC TTCAAACCCA AGACCCCATA AAACTGTTTG AGCCGTCGTT GCAACCTCCT
    GTAAGGCAGC AGCAGCCTCT CGAGAAAACA ATGAAGCCTT TCCCCATGGA GCCATATAAC
    CATAACCCCT CAGAAGTCAA GATCCCAGAG TTCTACTGGG ATTCTGCCTA CAGCGCTGCT
    GATAACAGAG CTGTGATGGC GCAGCAAGCC AGCGTGGACC GCAGGGGCAG ACGGTCCCCG
    GGAGTCTTCC GTCCAGAGCA GGACCCTGTG CCCAGGATGC CATTTGAGGA TCCCAAAGGC
    TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG
    CTGATTTTTT CTAACCCTCC TGATCTTTAC CCAGCTCTGC TGGGGCCTCT CGCCTCTCTT
    CCTGGACGAA GCCTCTTTAA ATCCTTACTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA
    TCCTCTTTCC TGTCCCTCAC CGGATTCTCC CTCAATCAGG AACGATACCC AAATAACAGT
    GTGTTCAATG AGGTATACGG GAAGAACCTG ACAACCAGCT CCAAAGCAGA ACTTAATCCC
    TCAATGGCTC CCCAGGAAAC ATCATTGTAC TCGCTTTTTG AAGGGACTCC ATGGTCTCCA
    TCTCTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCCAAC
    CCAAGCAGCC TGCCAAGCTC CCCTCCAACA CACAACCACA ATTCTGTCCC ATTCTCCAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGGAGAA CTGCAGACCG GTGGAAAACT
    GATAAGCCAG CCATGGGTGG GTTTGGCATC GATTATCTCT CAGCAACATC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CGCTCCAAGT GGCTCCTGGA CAGGCCATGG CCCATCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCCGCTTT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAGCGG
    GGACAAGGCG CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu65973
    Clone ID Related Accession (Same CDS sequence) XM_025285500.1
    Accession Version XM_025285500.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein SMG7 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT
    CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT
    AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG
    TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT
    GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCATCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA
    CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC
    ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC GACAGGCCGG
    AATCTAAGCA ACAGCTGTGA CACGGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATT
    AAGCCCCGAG AAGTGAACAG AGACCAAGGC AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAGTAGC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG
    GCAGTGCAGG TAAAATCTCA GACAGAGTTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA
    ACACCTGTAA CTCAAACCCC AAGTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGGTTCC CGCCCCCAAC ATATGTTATC
    CCCCCTCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCACCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCCG GACCAGGGCC AGTGACCCAG
    GGACCTCAGC AGCCTCAGCC ACCTTCCCAG CAGCCCCTCC CACCTCTGCC AGCTCCGCCA
    GCAGCACAGA CTGCAGGCCA GCTGCAGGTT CAGGCTCTAG CTCAGCAGCA ACAGTCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGT CCTCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATCATGC CTGTGAAACA GCCCTACTAC CTTCAAACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCGT TGCAACCTCC TGTAAGGCAG CAGCAGCCTC TCGAGAAAAC AATGAAGCCT
    TTCCCCATGG AGCCATATAA CCATAACCCC TCAGAAGTCA AGATCCCAGA GTTCTACTGG
    GATTCTGCCT ACAGCGCTGC TGATAACAGA GCTGTGATGG CGCAGCAAGC CAGCGTGGAC
    CGCAGGGGCA GACGGTCCCC GGGAGTCTTC CGTCCAGAGC AGGACCCTGT GCCCAGGATG
    CCATTTGAGA AATCCTTACT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC
    CTGTCCCTCA CCGGATTCTC CCTCAATCAG GAACGATACC CAAATAACAG TGTGTTCAAT
    GAGGTATACG GGAAGAACCT GACAACCAGC TCCAAAGCAG AACTTAATCC CTCAATGGCT
    CCCCAGGAAA CATCATTGTA CTCGCTTTTT GAAGGGACTC CATGGTCTCC ATCTCTTCCT
    GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCCAA CCCAAGCAGC
    CTGCCAAGCT CCCCTCCAAC ACACAACCAC AATTCTGTCC CATTCTCCAA TTTTGGACCC
    ATTGGGACTC CAGATAACAG GGATAGGAGA ACTGCAGACC GGTGGAAAAC TGATAAGCCA
    GCCATGGGTG GGTTTGGCAT CGATTATCTC TCAGCAACAT CATCCTCTGA GAGCAGTTGG
    CATCAGGCCA GCGCTCCAAG TGGCTCCTGG ACAGGCCATG GCCCATCCAT GGAGGATTCC
    TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA
    CCTTCCGCTT TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGACAAGGC
    GCCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025141285.1
    CDS142..3642
    Translation

    Target ORF information:

    RefSeq Version XM_025285500.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025285500.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT
    CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT
    AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG
    TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT
    GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCATCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA
    CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC
    ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC GACAGGCCGG
    AATCTAAGCA ACAGCTGTGA CACGGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATT
    AAGCCCCGAG AAGTGAACAG AGACCAAGGC AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAGTAGC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACAGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAGTGTG
    GCAGTGCAGG TAAAATCTCA GACAGAGTTA AGAAAGACTC CAGTGTCTGA AGCCAGGAAA
    ACACCTGTAA CTCAAACCCC AAGTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCCGG CCAGGGTTCC CGCCCCCAAC ATATGTTATC
    CCCCCTCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCACCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCCG GACCAGGGCC AGTGACCCAG
    GGACCTCAGC AGCCTCAGCC ACCTTCCCAG CAGCCCCTCC CACCTCTGCC AGCTCCGCCA
    GCAGCACAGA CTGCAGGCCA GCTGCAGGTT CAGGCTCTAG CTCAGCAGCA ACAGTCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGT CCTCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATCATGC CTGTGAAACA GCCCTACTAC CTTCAAACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCGT TGCAACCTCC TGTAAGGCAG CAGCAGCCTC TCGAGAAAAC AATGAAGCCT
    TTCCCCATGG AGCCATATAA CCATAACCCC TCAGAAGTCA AGATCCCAGA GTTCTACTGG
    GATTCTGCCT ACAGCGCTGC TGATAACAGA GCTGTGATGG CGCAGCAAGC CAGCGTGGAC
    CGCAGGGGCA GACGGTCCCC GGGAGTCTTC CGTCCAGAGC AGGACCCTGT GCCCAGGATG
    CCATTTGAGA AATCCTTACT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC
    CTGTCCCTCA CCGGATTCTC CCTCAATCAG GAACGATACC CAAATAACAG TGTGTTCAAT
    GAGGTATACG GGAAGAACCT GACAACCAGC TCCAAAGCAG AACTTAATCC CTCAATGGCT
    CCCCAGGAAA CATCATTGTA CTCGCTTTTT GAAGGGACTC CATGGTCTCC ATCTCTTCCT
    GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCCAA CCCAAGCAGC
    CTGCCAAGCT CCCCTCCAAC ACACAACCAC AATTCTGTCC CATTCTCCAA TTTTGGACCC
    ATTGGGACTC CAGATAACAG GGATAGGAGA ACTGCAGACC GGTGGAAAAC TGATAAGCCA
    GCCATGGGTG GGTTTGGCAT CGATTATCTC TCAGCAACAT CATCCTCTGA GAGCAGTTGG
    CATCAGGCCA GCGCTCCAAG TGGCTCCTGG ACAGGCCATG GCCCATCCAT GGAGGATTCC
    TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA
    CCTTCCGCTT TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAGCG GGGACAAGGC
    GCCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu65974
    Clone ID Related Accession (Same CDS sequence) XM_025285501.1
    Accession Version XM_025285501.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein SMG7 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT
    CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT
    AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG
    TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT
    GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCATCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA
    CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC
    ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC GACAGGCCGG
    AATCTAAGCA ACAGCTGTGA CACGGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATT
    AAGCCCCGAG AAGTGAACAG AGACCAAGGC AGAAGTTTTC CTCCCAAAGA GGTAAAATCT
    CAGACAGAGT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACACCTGT AACTCAAACC
    CCAAGTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCC GGCCAGGGTT CCCGCCCCCA ACATATGTTA TCCCCCCTCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCACC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC CGGACCAGGG CCAGTGACCC AGGGACCTCA GCAGCCTCAG
    CCACCTTCCC AGCAGCCCCT CCCACCTCTG CCAGCTCCGC CAGCAGCACA GACTGCAGGC
    CAGCTGCAGG TTCAGGCTCT AGCTCAGCAG CAACAGTCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GTCCTCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATCAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAAAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC GTTGCAACCT
    CCTGTAAGGC AGCAGCAGCC TCTCGAGAAA ACAATGAAGC CTTTCCCCAT GGAGCCATAT
    AACCATAACC CCTCAGAAGT CAAGATCCCA GAGTTCTACT GGGATTCTGC CTACAGCGCT
    GCTGATAACA GAGCTGTGAT GGCGCAGCAA GCCAGCGTGG ACCGCAGGGG CAGACGGTCC
    CCGGGAGTCT TCCGTCCAGA GCAGGACCCT GTGCCCAGGA TGCCATTTGA GAAATCCTTA
    CTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC
    TCCCTCAATC AGGAACGATA CCCAAATAAC AGTGTGTTCA ATGAGGTATA CGGGAAGAAC
    CTGACAACCA GCTCCAAAGC AGAACTTAAT CCCTCAATGG CTCCCCAGGA AACATCATTG
    TACTCGCTTT TTGAAGGGAC TCCATGGTCT CCATCTCTTC CTGCCAGTTC AGATCATTCA
    ACACCAGCCA GCCAGTCTCC TCATTCCTCC AACCCAAGCA GCCTGCCAAG CTCCCCTCCA
    ACACACAACC ACAATTCTGT CCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC
    AGGGATAGGA GAACTGCAGA CCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC
    ATCGATTATC TCTCAGCAAC ATCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCGCTCCA
    AGTGGCTCCT GGACAGGCCA TGGCCCATCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA
    AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCCGC TTTGGAGCAG
    CTGTTAATGC AGCAGAAGCA GAAACAGCAG CGGGGACAAG GCGCCATGAA CCCTCCACAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025141286.1
    CDS142..3504
    Translation

    Target ORF information:

    RefSeq Version XM_025285501.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025285501.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGGAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCCGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTTG TTACTGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCATGCCT TTAAGAATCA GATCACAACA
    CTACAAGGCC AGGCAAAGAA TCGAGCAAAC CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTGTTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AGTTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTTCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC GGCTCAGCTT GTCCCCTCTA ATGGTCAGCC TTACAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACTA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCTACTA ATCTACAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGGGA TGAGGTGAAA ACCAAATGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AGTTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAGAAAGC TTTCAACTCT
    CAGCAGTTAG TTCACGTCAC TGTCATTAAC CTGTTTCAAC TTCATCATCT TCGTGACTTT
    AGCAACGAAA CGGAGCAGCA TAGTTATAGC CAAGATGAGC AGCTGTGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTCCA GAACAAGTCT
    CAGGAGGAAT CCTACAATGC CTATCCCCTA CCTGCCGTCA AGGTGTCCAT GGACTGGCTG
    AGACTCAGAC CCAGGGTGTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCG
    TGGCTAATTT CTCTTCTGAA CAGTTTCCAT CCCCATGAAG AAGATCTTTC AAGTACTAAT
    GCAACACCAC TTCCAGAGGA GTTTGAGTTA CAAGGATTCT TGGCTTTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCATCAG GGTATTACAG GAGACAAAGA GGGTCAGCAA
    CGACAAATAC GACAGCAGCG TTTGATCTCT ATAGGCAAAT GGATTGCAGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAG
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA CACAACCATC
    ATAGAGTCCC TGGCAGCTGA CGGGAACCCA GGACTGAAAT CAGTGCTGTC GACAGGCCGG
    AATCTAAGCA ACAGCTGTGA CACGGGAGAG AAACCAGTGG TCACCTTCAA AGAGAACATT
    AAGCCCCGAG AAGTGAACAG AGACCAAGGC AGAAGTTTTC CTCCCAAAGA GGTAAAATCT
    CAGACAGAGT TAAGAAAGAC TCCAGTGTCT GAAGCCAGGA AAACACCTGT AACTCAAACC
    CCAAGTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCC GGCCAGGGTT CCCGCCCCCA ACATATGTTA TCCCCCCTCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCACC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC CGGACCAGGG CCAGTGACCC AGGGACCTCA GCAGCCTCAG
    CCACCTTCCC AGCAGCCCCT CCCACCTCTG CCAGCTCCGC CAGCAGCACA GACTGCAGGC
    CAGCTGCAGG TTCAGGCTCT AGCTCAGCAG CAACAGTCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GTCCTCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATCAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAAAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC GTTGCAACCT
    CCTGTAAGGC AGCAGCAGCC TCTCGAGAAA ACAATGAAGC CTTTCCCCAT GGAGCCATAT
    AACCATAACC CCTCAGAAGT CAAGATCCCA GAGTTCTACT GGGATTCTGC CTACAGCGCT
    GCTGATAACA GAGCTGTGAT GGCGCAGCAA GCCAGCGTGG ACCGCAGGGG CAGACGGTCC
    CCGGGAGTCT TCCGTCCAGA GCAGGACCCT GTGCCCAGGA TGCCATTTGA GAAATCCTTA
    CTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC
    TCCCTCAATC AGGAACGATA CCCAAATAAC AGTGTGTTCA ATGAGGTATA CGGGAAGAAC
    CTGACAACCA GCTCCAAAGC AGAACTTAAT CCCTCAATGG CTCCCCAGGA AACATCATTG
    TACTCGCTTT TTGAAGGGAC TCCATGGTCT CCATCTCTTC CTGCCAGTTC AGATCATTCA
    ACACCAGCCA GCCAGTCTCC TCATTCCTCC AACCCAAGCA GCCTGCCAAG CTCCCCTCCA
    ACACACAACC ACAATTCTGT CCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC
    AGGGATAGGA GAACTGCAGA CCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC
    ATCGATTATC TCTCAGCAAC ATCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCGCTCCA
    AGTGGCTCCT GGACAGGCCA TGGCCCATCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA
    AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCCGC TTTGGAGCAG
    CTGTTAATGC AGCAGAAGCA GAAACAGCAG CGGGGACAAG GCGCCATGAA CCCTCCACAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.