CRIM1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRIM1
Entrez Gene ID	102395417
Full Name	cysteine rich transmembrane BMP regulator 1
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006056853.1	XP_006056915.1	cysteine-rich motor neuron 1 protein isoform X2
XM_006056853.2	XP_006056915.1	cysteine-rich motor neuron 1 protein isoform X2
XM_006056852.1	XP_006056914.1	cysteine-rich motor neuron 1 protein isoform X1
XM_006056854.1	XP_006056916.1	cysteine-rich motor neuron 1 protein isoform X3
XM_006056852.2	XP_006056914.1	cysteine-rich motor neuron 1 protein isoform X1
XM_006056855.2	XP_006056917.1	cysteine-rich motor neuron 1 protein isoform X3
XM_006056855.1	XP_006056917.1	cysteine-rich motor neuron 1 protein isoform X4

Pathways from BioSystems
Gene Ontology

The following CRIM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRIM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu13903	XM_006056853.1 Latest version!	Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu13904	XM_006056854.1 Latest version!	Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu13905	XM_006056855.1 Latest version!	Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu45932	XM_006056853.2 Latest version!	Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (CRIM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu45933	XM_006056855.2 Latest version!	Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (CRIM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu13902	XM_006056852.1 Latest version!	Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu45931	XM_006056852.2 Latest version!	Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (CRIM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRIM1 ( XM_006056853.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006056915.1 Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu13903
Clone ID Related Accession (Same CDS sequence)	XM_006056853.1
Accession Version	XM_006056853.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	cysteine-rich motor neuron 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784779.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCT GGTCTCGCTG 
CTGGGGCTGT TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAAGA GCCCAAGAGC TGCCCCGGGA GCATCGTGCA GGGCTTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGA AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GAAGAGAAGC CAGATTGCTC CAAGGCCCGC 
TGTGAAGTCC AGTTCTCTCC ACGTTGTCCT GAAGACTCCG TTCTGATCGA GGGCTACGCG 
CCCCCCGGGG AGTGCTGCCC CCTCCCCAGC CGCTGTGTGT GTGACCCCGC GGGCTGCCTG 
CGGAAAGTCT GCCAGCCAGG ATACCTGAAC ATCCTTGTGT CCAAAGCCTC AGGGAAGCCG 
GGAGAGTGCT GTGACCTCTA TGAGTGCAAA CCAGTTTTCA GTGTGGACTG CAGCACCGTG 
GAGTGCCCCT CCTTTCAGCA AACCGTGTGC CCCCTGGACA GCTACGAAAC CCAAGTCAGA 
CTCACAGCGG ATGGCTGCTG CACTCTTCCA ACAAGATGCG AGTGTCTCTC TGGCTTATGT 
GGTTTCCCCG TGTGTGAGGT GGGATCCACT CCCCGCATAG TCTCTCGTGG AGATGGGACA 
CCTGGAAAGT GCTGTGATGT CTTTGAATGT GTTAATGAAA CAAAGCCAGC CTGCGTGTTC 
AACAACGTGG AGTACAGTGA CGGAGACATG TTTCGAATGG ACAACTGTCG ATTCTGTCGA 
TGCCAAGGGG GTGTTTCCAT CTGCTTCACT GCCCAGTGTG GTGAGCTGAG CTGTGAGAGA 
TACTACATGC CCGAAGGGGA GTGCTGCCCT GTGTGTGAAG ATCCTGTGTA TCCTTTGAAC 
AACCCCGCTG GCTGCTATGC CAATGGCCAG ATCCACGCCC ACGGGGACCG GTGGCGGGAA 
GACGACTGCA CGTTCTGCCA GTGCATCAAT GGGGAGCCCC ACTGCGTGGC GACGGCCTGT 
GGACAGAGCT GCATGAACCC CGTCAAAGTG CCTGGGGAGT GCTGCCCTGT ATGCGAAGAA 
CCAACCTACA TCACAATTGA TCCACCTGCA TGTGGGGAGT TATCAAACTG TACCCTGACA 
GAGAAGGACT GCATTTATGG TTTCAAACTC GATCACAATG GTTGTCGAAC CTGTCAGTGC 
AAAAACAGGG AGGAGCTCTG CACGGGCCTC AGGCGAGGCT GCGCCTTGAA CTGCCCCTTC 
GGCTTCCTAA CCGACGCCTA CGGCTGTGAC GCCTGTCAGT GCCGCCCGCG GCCCCGGAAG 
TGCAGACCCA CAGCCTGCGA AAAGCATTGT CCGTTCGGAT ACCTGAAGAA TAAGCACGGC 
TGTGACATCT GTCGCTGTAA GAAATGCCCA GAGCTCCGGT GCAGTAAGAT CTGCTCCCTG 
GGTTTCCAGA AGGACAGTCA TGGCTGTCTC ATCTGCAAGT GCAGAGAGGT CTCTGCCTCC 
GCCGGGCCAC CCGTCCTGTC AGGCACGTGT CTATCCATGG ATGGCCATCA TCATAAAAAT 
GAGGAGAGCT GGCATGATGG GTGTCGGGAA TGCTACTGCC ACAGTGGACG GGAGATGTGC 
GCACTGATCA CCTGCCCCGT GCCTGCCTGT GGCAACCCCA CCATTCACCC GGGACGGTGC 
TGCCCATCAT GTTCAGATGA CTTCGTGGTG CAGAAGCCAG AGCTCAGCAC GCCCTCCATC 
TGCCACGCCC CCGGGGGAGA GTACTTTGTG GAGGGAGAAA CGTGGCACAT TGACACCTGC 
ACGCAATGCA CGTGCCACAG CGGGCGGGTG CTGTGTGAGA CGGAGGTGTG CCCGCCGCTG 
CTCTGCCAGA ACCCCACACG CACCCAGGAC TCCTGCTGCC CACAGTGCCC AGATGAACCT 
CTCCAGCCTT CCTCATCCCA CAATGACAGT GTGCCTAGTT ACTGCAAAAA CGATGAAGGG 
GATATATTCC TGGCAGCTGA GTCCTGGAAA CCCGACGTGT GTACCAGCTG CGTGTGCATG 
GATAGCGTCA TTAGCTGTTA CTCTGAGTCT TGCCCTTCTG TATCCTGTGA AAGACCTGTG 
TTGAGAAAAG GCCAGTGTTG TCCCTACTGC ATAGAGGACA CAATCCCAAA GAAGGTGGTG 
TGCCACTTCA GCGGGAAGGC CTACGCTGAC GAGGAGCGCT GGGACATCGA CAGCTGCACG 
CACTGCTACT GCCTGCAGGG CCAGACCCTC TGCTCCACCG TCAGCTGCCC GCCGCTGCCC 
TGCGTCGAGC CCATCAACGT GGAAGGCAGC TGCTGCCCCA TGTGCCCAGA AATGTATGTC 
CCAGAACCAA CCAATATACC CATTGAGAAG ACAAATCATC GGGAGATCGA CATGGAGGTC 
CCCCTGTGGC CCACACCTAG TGAAAATGAC ATTATCCATC TCCCCAGAGA CATGGGTCAC 
CCCCAGATAG ATTACAGAGA TAATACCAGG CTGAACTCAG AAGATTCTTC AGTGGACTCC 
ATTGCCTCGG TTGTGGTTCC CATAATAATA GGCCTCTCTA TCATAATAGC ATTCCTGTTC 
ATCAATCAGA AGAAACAGTG GATACCACTG CTTTGCTGGT ATCGAACACC AACTAAGCCT 
TCTTCTTTAA ATAATCAGCT GGTATCTGTG GACTGCAAGA AAGGAACCCG GGTCCAGGTG 
GACAGTTCCC AGAGAATGCT GAGAATCGCG GAACCAGACG CGAGATTCAG TGGCTTCTAC 
AGCATGCAGA AACAGAACCA CCTACAGGCA GACAATTTCT ACCAAACAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006056915.1
CDS	60..2993
Translation	MYLVAGGRGLAGCGHLLVSLLGLLLLLARSGTRALVCLPCDESKCEEPKSCPGSIVQGFCGCCYMCARQRNESCGGAYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEEEKPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCDPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPSFQQTVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPVCEVGSTPRIVSRGDGTPGKCCDVFECVNETKPACVFNNVEYSDGDMFRMDNCRFCRCQGGVSICFTAQCGELSCERYYMPEGECCPVCEDPVYPLNNPAGCYANGQIHAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLTEKDCIYGFKLDHNGCRTCQCKNREELCTGLRRGCALNCPFGFLTDAYGCDACQCRPRPRKCRPTACEKHCPFGYLKNKHGCDICRCKKCPELRCSKICSLGFQKDSHGCLICKCREVSASAGPPVLSGTCLSMDGHHHKNEESWHDGCRECYCHSGREMCALITCPVPACGNPTIHPGRCCPSCSDDFVVQKPELSTPSICHAPGGEYFVEGETWHIDTCTQCTCHSGRVLCETEVCPPLLCQNPTRTQDSCCPQCPDEPLQPSSSHNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCYSESCPSVSCERPVLRKGQCCPYCIEDTIPKKVVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHREIDMEVPLWPTPSENDIIHLPRDMGHPQIDYRDNTRLNSEDSSVDSIASVVVPIIIGLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGTRVQVDSSQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_006056853.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006056853.1

ORF Insert Sequence:

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCT GGTCTCGCTG 
CTGGGGCTGT TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAAGA GCCCAAGAGC TGCCCCGGGA GCATCGTGCA GGGCTTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGA AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GAAGAGAAGC CAGATTGCTC CAAGGCCCGC 
TGTGAAGTCC AGTTCTCTCC ACGTTGTCCT GAAGACTCCG TTCTGATCGA GGGCTACGCG 
CCCCCCGGGG AGTGCTGCCC CCTCCCCAGC CGCTGTGTGT GTGACCCCGC GGGCTGCCTG 
CGGAAAGTCT GCCAGCCAGG ATACCTGAAC ATCCTTGTGT CCAAAGCCTC AGGGAAGCCG 
GGAGAGTGCT GTGACCTCTA TGAGTGCAAA CCAGTTTTCA GTGTGGACTG CAGCACCGTG 
GAGTGCCCCT CCTTTCAGCA AACCGTGTGC CCCCTGGACA GCTACGAAAC CCAAGTCAGA 
CTCACAGCGG ATGGCTGCTG CACTCTTCCA ACAAGATGCG AGTGTCTCTC TGGCTTATGT 
GGTTTCCCCG TGTGTGAGGT GGGATCCACT CCCCGCATAG TCTCTCGTGG AGATGGGACA 
CCTGGAAAGT GCTGTGATGT CTTTGAATGT GTTAATGAAA CAAAGCCAGC CTGCGTGTTC 
AACAACGTGG AGTACAGTGA CGGAGACATG TTTCGAATGG ACAACTGTCG ATTCTGTCGA 
TGCCAAGGGG GTGTTTCCAT CTGCTTCACT GCCCAGTGTG GTGAGCTGAG CTGTGAGAGA 
TACTACATGC CCGAAGGGGA GTGCTGCCCT GTGTGTGAAG ATCCTGTGTA TCCTTTGAAC 
AACCCCGCTG GCTGCTATGC CAATGGCCAG ATCCACGCCC ACGGGGACCG GTGGCGGGAA 
GACGACTGCA CGTTCTGCCA GTGCATCAAT GGGGAGCCCC ACTGCGTGGC GACGGCCTGT 
GGACAGAGCT GCATGAACCC CGTCAAAGTG CCTGGGGAGT GCTGCCCTGT ATGCGAAGAA 
CCAACCTACA TCACAATTGA TCCACCTGCA TGTGGGGAGT TATCAAACTG TACCCTGACA 
GAGAAGGACT GCATTTATGG TTTCAAACTC GATCACAATG GTTGTCGAAC CTGTCAGTGC 
AAAAACAGGG AGGAGCTCTG CACGGGCCTC AGGCGAGGCT GCGCCTTGAA CTGCCCCTTC 
GGCTTCCTAA CCGACGCCTA CGGCTGTGAC GCCTGTCAGT GCCGCCCGCG GCCCCGGAAG 
TGCAGACCCA CAGCCTGCGA AAAGCATTGT CCGTTCGGAT ACCTGAAGAA TAAGCACGGC 
TGTGACATCT GTCGCTGTAA GAAATGCCCA GAGCTCCGGT GCAGTAAGAT CTGCTCCCTG 
GGTTTCCAGA AGGACAGTCA TGGCTGTCTC ATCTGCAAGT GCAGAGAGGT CTCTGCCTCC 
GCCGGGCCAC CCGTCCTGTC AGGCACGTGT CTATCCATGG ATGGCCATCA TCATAAAAAT 
GAGGAGAGCT GGCATGATGG GTGTCGGGAA TGCTACTGCC ACAGTGGACG GGAGATGTGC 
GCACTGATCA CCTGCCCCGT GCCTGCCTGT GGCAACCCCA CCATTCACCC GGGACGGTGC 
TGCCCATCAT GTTCAGATGA CTTCGTGGTG CAGAAGCCAG AGCTCAGCAC GCCCTCCATC 
TGCCACGCCC CCGGGGGAGA GTACTTTGTG GAGGGAGAAA CGTGGCACAT TGACACCTGC 
ACGCAATGCA CGTGCCACAG CGGGCGGGTG CTGTGTGAGA CGGAGGTGTG CCCGCCGCTG 
CTCTGCCAGA ACCCCACACG CACCCAGGAC TCCTGCTGCC CACAGTGCCC AGATGAACCT 
CTCCAGCCTT CCTCATCCCA CAATGACAGT GTGCCTAGTT ACTGCAAAAA CGATGAAGGG 
GATATATTCC TGGCAGCTGA GTCCTGGAAA CCCGACGTGT GTACCAGCTG CGTGTGCATG 
GATAGCGTCA TTAGCTGTTA CTCTGAGTCT TGCCCTTCTG TATCCTGTGA AAGACCTGTG 
TTGAGAAAAG GCCAGTGTTG TCCCTACTGC ATAGAGGACA CAATCCCAAA GAAGGTGGTG 
TGCCACTTCA GCGGGAAGGC CTACGCTGAC GAGGAGCGCT GGGACATCGA CAGCTGCACG 
CACTGCTACT GCCTGCAGGG CCAGACCCTC TGCTCCACCG TCAGCTGCCC GCCGCTGCCC 
TGCGTCGAGC CCATCAACGT GGAAGGCAGC TGCTGCCCCA TGTGCCCAGA AATGTATGTC 
CCAGAACCAA CCAATATACC CATTGAGAAG ACAAATCATC GGGAGATCGA CATGGAGGTC 
CCCCTGTGGC CCACACCTAG TGAAAATGAC ATTATCCATC TCCCCAGAGA CATGGGTCAC 
CCCCAGATAG ATTACAGAGA TAATACCAGG CTGAACTCAG AAGATTCTTC AGTGGACTCC 
ATTGCCTCGG TTGTGGTTCC CATAATAATA GGCCTCTCTA TCATAATAGC ATTCCTGTTC 
ATCAATCAGA AGAAACAGTG GATACCACTG CTTTGCTGGT ATCGAACACC AACTAAGCCT 
TCTTCTTTAA ATAATCAGCT GGTATCTGTG GACTGCAAGA AAGGAACCCG GGTCCAGGTG 
GACAGTTCCC AGAGAATGCT GAGAATCGCG GAACCAGACG CGAGATTCAG TGGCTTCTAC 
AGCATGCAGA AACAGAACCA CCTACAGGCA GACAATTTCT ACCAAACAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRIM1 ( XM_006056854.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006056916.1 Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu13904
Clone ID Related Accession (Same CDS sequence)	XM_006056854.1
Accession Version	XM_006056854.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	cysteine-rich motor neuron 1 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784779.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTCAA GCCTCAGTGA TGATGTCCAC ACCAGCTCCT TGACTGGCTC TGGGAGTCCT 
GGGAGCAGTG GCCCTGAGGC TAAGTGGAGA TGTCTTGGTT TTCAGACTCA CTGGTCCTGC 
AAACCCCAGG ATGAGAACTG GGATGATGAC CAACTGCTTG GGTTTGAACC ATGCAATGAA 
AACCTTGTCT CTGGCTGCAA TCTCATCAAT GGAAAATGTG AGTGTGACAC CATTCGAACC 
TGTAACAATC CCTTCGAGTT TCCAAGGAAG GATATGTGCC TTTCAGCTTT AAAGAGGATT 
GAAGAAGAGA AGCCAGATTG CTCCAAGGCC CGCTGTGAAG TCCAGTTCTC TCCACGTTGT 
CCTGAAGACT CCGTTCTGAT CGAGGGCTAC GCGCCCCCCG GGGAGTGCTG CCCCCTCCCC 
AGCCGCTGTG TGTGTGACCC CGCGGGCTGC CTGCGGAAAG TCTGCCAGCC AGGATACCTG 
AACATCCTTG TGTCCAAAGC CTCAGGGAAG CCGGGAGAGT GCTGTGACCT CTATGAGTGC 
AAACCAGTTT TCAGTGTGGA CTGCAGCACC GTGGAGTGCC CCTCCTTTCA GCAAACCGTG 
TGCCCCCTGG ACAGCTACGA AACCCAAGTC AGACTCACAG CGGATGGCTG CTGCACTCTT 
CCAACAAGAT GCGAGTGTCT CTCTGGCTTA TGTGGTTTCC CCGTGTGTGA GGTGGGATCC 
ACTCCCCGCA TAGTCTCTCG TGGAGATGGG ACACCTGGAA AGTGCTGTGA TGTCTTTGAA 
TGTGTTAATG AAACAAAGCC AGCCTGCGTG TTCAACAACG TGGAGTACAG TGACGGAGAC 
ATGTTTCGAA TGGACAACTG TCGATTCTGT CGATGCCAAG GGGGTGTTTC CATCTGCTTC 
ACTGCCCAGT GTGGTGAGCT GAGCTGTGAG AGATACTACA TGCCCGAAGG GGAGTGCTGC 
CCTGTGTGTG AAGATCCTGT GTATCCTTTG AACAACCCCG CTGGCTGCTA TGCCAATGGC 
CAGATCCACG CCCACGGGGA CCGGTGGCGG GAAGACGACT GCACGTTCTG CCAGTGCATC 
AATGGGGAGC CCCACTGCGT GGCGACGGCC TGTGGACAGA GCTGCATGAA CCCCGTCAAA 
GTGCCTGGGG AGTGCTGCCC TGTATGCGAA GAACCAACCT ACATCACAAT TGATCCACCT 
GCATGTGGGG AGTTATCAAA CTGTACCCTG ACAGAGAAGG ACTGCATTTA TGGTTTCAAA 
CTCGATCACA ATGGTTGTCG AACCTGTCAG TGCAAAAACA GGGAGGAGCT CTGCACGGGC 
CTCAGGCGAG GCTGCGCCTT GAACTGCCCC TTCGGCTTCC TAACCGACGC CTACGGCTGT 
GACGCCTGTC AGTGCCGCCC GCGGCCCCGG AAGTGCAGAC CCACAGCCTG CGAAAAGCAT 
TGTCCGTTCG GATACCTGAA GAATAAGCAC GGCTGTGACA TCTGTCGCTG TAAGAAATGC 
CCAGAGCTCC GGTGCAGTAA GATCTGCTCC CTGGGTTTCC AGAAGGACAG TCATGGCTGT 
CTCATCTGCA AGTGCAGAGA GGTCTCTGCC TCCGCCGGGC CACCCGTCCT GTCAGGCACG 
TGTCTATCCA TGGATGGCCA TCATCATAAA AATGAGGAGA GCTGGCATGA TGGGTGTCGG 
GAATGCTACT GCCACAGTGG ACGGGAGATG TGCGCACTGA TCACCTGCCC CGTGCCTGCC 
TGTGGCAACC CCACCATTCA CCCGGGACGG TGCTGCCCAT CATGTTCAGA TGACTTCGTG 
GTGCAGAAGC CAGAGCTCAG CACGCCCTCC ATCTGCCACG CCCCCGGGGG AGAGTACTTT 
GTGGAGGGAG AAACGTGGCA CATTGACACC TGCACGCAAT GCACGTGCCA CAGCGGGCGG 
GTGCTGTGTG AGACGGAGGT GTGCCCGCCG CTGCTCTGCC AGAACCCCAC ACGCACCCAG 
GACTCCTGCT GCCCACAGTG CCCAGATGAA CCTCTCCAGC CTTCCTCATC CCACAATGAC 
AGTGTGCCTA GTTACTGCAA AAACGATGAA GGGGATATAT TCCTGGCAGC TGAGTCCTGG 
AAACCCGACG TGTGTACCAG CTGCGTGTGC ATGGATAGCG TCATTAGCTG TTACTCTGAG 
TCTTGCCCTT CTGTATCCTG TGAAAGACCT GTGTTGAGAA AAGGCCAGTG TTGTCCCTAC 
TGCATAGAGG ACACAATCCC AAAGAAGGTG GTGTGCCACT TCAGCGGGAA GGCCTACGCT 
GACGAGGAGC GCTGGGACAT CGACAGCTGC ACGCACTGCT ACTGCCTGCA GGGCCAGACC 
CTCTGCTCCA CCGTCAGCTG CCCGCCGCTG CCCTGCGTCG AGCCCATCAA CGTGGAAGGC 
AGCTGCTGCC CCATGTGCCC AGAAATGTAT GTCCCAGAAC CAACCAATAT ACCCATTGAG 
AAGACAAATC ATCGGGAGAT CGACATGGAG GTCCCCCTGT GGCCCACACC TAGTGAAAAT 
GACATTATCC ATCTCCCCAG AGACATGGGT CACCCCCAGA TAGATTACAG AGATAATACC 
AGGCTGAACT CAGAAGATTC TTCAGTGGAC TCCATTGCCT CGGTTGTGGT TCCCATAATA 
ATAGGCCTCT CTATCATAAT AGCATTCCTG TTCATCAATC AGAAGAAACA GTGGATACCA 
CTGCTTTGCT GGTATCGAAC ACCAACTAAG CCTTCTTCTT TAAATAATCA GCTGGTATCT 
GTGGACTGCA AGAAAGGAAC CCGGGTCCAG GTGGACAGTT CCCAGAGAAT GCTGAGAATC 
GCGGAACCAG ACGCGAGATT CAGTGGCTTC TACAGCATGC AGAAACAGAA CCACCTACAG 
GCAGACAATT TCTACCAAAC AGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006056916.1
CDS	18..2924
Translation	MRSSLSDDVHTSSLTGSGSPGSSGPEAKWRCLGFQTHWSCKPQDENWDDDQLLGFEPCNENLVSGCNLINGKCECDTIRTCNNPFEFPRKDMCLSALKRIEEEKPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCDPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPSFQQTVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPVCEVGSTPRIVSRGDGTPGKCCDVFECVNETKPACVFNNVEYSDGDMFRMDNCRFCRCQGGVSICFTAQCGELSCERYYMPEGECCPVCEDPVYPLNNPAGCYANGQIHAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLTEKDCIYGFKLDHNGCRTCQCKNREELCTGLRRGCALNCPFGFLTDAYGCDACQCRPRPRKCRPTACEKHCPFGYLKNKHGCDICRCKKCPELRCSKICSLGFQKDSHGCLICKCREVSASAGPPVLSGTCLSMDGHHHKNEESWHDGCRECYCHSGREMCALITCPVPACGNPTIHPGRCCPSCSDDFVVQKPELSTPSICHAPGGEYFVEGETWHIDTCTQCTCHSGRVLCETEVCPPLLCQNPTRTQDSCCPQCPDEPLQPSSSHNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCYSESCPSVSCERPVLRKGQCCPYCIEDTIPKKVVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHREIDMEVPLWPTPSENDIIHLPRDMGHPQIDYRDNTRLNSEDSSVDSIASVVVPIIIGLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGTRVQVDSSQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_006056854.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006056854.1

ORF Insert Sequence:

ATGAGGTCAA GCCTCAGTGA TGATGTCCAC ACCAGCTCCT TGACTGGCTC TGGGAGTCCT 
GGGAGCAGTG GCCCTGAGGC TAAGTGGAGA TGTCTTGGTT TTCAGACTCA CTGGTCCTGC 
AAACCCCAGG ATGAGAACTG GGATGATGAC CAACTGCTTG GGTTTGAACC ATGCAATGAA 
AACCTTGTCT CTGGCTGCAA TCTCATCAAT GGAAAATGTG AGTGTGACAC CATTCGAACC 
TGTAACAATC CCTTCGAGTT TCCAAGGAAG GATATGTGCC TTTCAGCTTT AAAGAGGATT 
GAAGAAGAGA AGCCAGATTG CTCCAAGGCC CGCTGTGAAG TCCAGTTCTC TCCACGTTGT 
CCTGAAGACT CCGTTCTGAT CGAGGGCTAC GCGCCCCCCG GGGAGTGCTG CCCCCTCCCC 
AGCCGCTGTG TGTGTGACCC CGCGGGCTGC CTGCGGAAAG TCTGCCAGCC AGGATACCTG 
AACATCCTTG TGTCCAAAGC CTCAGGGAAG CCGGGAGAGT GCTGTGACCT CTATGAGTGC 
AAACCAGTTT TCAGTGTGGA CTGCAGCACC GTGGAGTGCC CCTCCTTTCA GCAAACCGTG 
TGCCCCCTGG ACAGCTACGA AACCCAAGTC AGACTCACAG CGGATGGCTG CTGCACTCTT 
CCAACAAGAT GCGAGTGTCT CTCTGGCTTA TGTGGTTTCC CCGTGTGTGA GGTGGGATCC 
ACTCCCCGCA TAGTCTCTCG TGGAGATGGG ACACCTGGAA AGTGCTGTGA TGTCTTTGAA 
TGTGTTAATG AAACAAAGCC AGCCTGCGTG TTCAACAACG TGGAGTACAG TGACGGAGAC 
ATGTTTCGAA TGGACAACTG TCGATTCTGT CGATGCCAAG GGGGTGTTTC CATCTGCTTC 
ACTGCCCAGT GTGGTGAGCT GAGCTGTGAG AGATACTACA TGCCCGAAGG GGAGTGCTGC 
CCTGTGTGTG AAGATCCTGT GTATCCTTTG AACAACCCCG CTGGCTGCTA TGCCAATGGC 
CAGATCCACG CCCACGGGGA CCGGTGGCGG GAAGACGACT GCACGTTCTG CCAGTGCATC 
AATGGGGAGC CCCACTGCGT GGCGACGGCC TGTGGACAGA GCTGCATGAA CCCCGTCAAA 
GTGCCTGGGG AGTGCTGCCC TGTATGCGAA GAACCAACCT ACATCACAAT TGATCCACCT 
GCATGTGGGG AGTTATCAAA CTGTACCCTG ACAGAGAAGG ACTGCATTTA TGGTTTCAAA 
CTCGATCACA ATGGTTGTCG AACCTGTCAG TGCAAAAACA GGGAGGAGCT CTGCACGGGC 
CTCAGGCGAG GCTGCGCCTT GAACTGCCCC TTCGGCTTCC TAACCGACGC CTACGGCTGT 
GACGCCTGTC AGTGCCGCCC GCGGCCCCGG AAGTGCAGAC CCACAGCCTG CGAAAAGCAT 
TGTCCGTTCG GATACCTGAA GAATAAGCAC GGCTGTGACA TCTGTCGCTG TAAGAAATGC 
CCAGAGCTCC GGTGCAGTAA GATCTGCTCC CTGGGTTTCC AGAAGGACAG TCATGGCTGT 
CTCATCTGCA AGTGCAGAGA GGTCTCTGCC TCCGCCGGGC CACCCGTCCT GTCAGGCACG 
TGTCTATCCA TGGATGGCCA TCATCATAAA AATGAGGAGA GCTGGCATGA TGGGTGTCGG 
GAATGCTACT GCCACAGTGG ACGGGAGATG TGCGCACTGA TCACCTGCCC CGTGCCTGCC 
TGTGGCAACC CCACCATTCA CCCGGGACGG TGCTGCCCAT CATGTTCAGA TGACTTCGTG 
GTGCAGAAGC CAGAGCTCAG CACGCCCTCC ATCTGCCACG CCCCCGGGGG AGAGTACTTT 
GTGGAGGGAG AAACGTGGCA CATTGACACC TGCACGCAAT GCACGTGCCA CAGCGGGCGG 
GTGCTGTGTG AGACGGAGGT GTGCCCGCCG CTGCTCTGCC AGAACCCCAC ACGCACCCAG 
GACTCCTGCT GCCCACAGTG CCCAGATGAA CCTCTCCAGC CTTCCTCATC CCACAATGAC 
AGTGTGCCTA GTTACTGCAA AAACGATGAA GGGGATATAT TCCTGGCAGC TGAGTCCTGG 
AAACCCGACG TGTGTACCAG CTGCGTGTGC ATGGATAGCG TCATTAGCTG TTACTCTGAG 
TCTTGCCCTT CTGTATCCTG TGAAAGACCT GTGTTGAGAA AAGGCCAGTG TTGTCCCTAC 
TGCATAGAGG ACACAATCCC AAAGAAGGTG GTGTGCCACT TCAGCGGGAA GGCCTACGCT 
GACGAGGAGC GCTGGGACAT CGACAGCTGC ACGCACTGCT ACTGCCTGCA GGGCCAGACC 
CTCTGCTCCA CCGTCAGCTG CCCGCCGCTG CCCTGCGTCG AGCCCATCAA CGTGGAAGGC 
AGCTGCTGCC CCATGTGCCC AGAAATGTAT GTCCCAGAAC CAACCAATAT ACCCATTGAG 
AAGACAAATC ATCGGGAGAT CGACATGGAG GTCCCCCTGT GGCCCACACC TAGTGAAAAT 
GACATTATCC ATCTCCCCAG AGACATGGGT CACCCCCAGA TAGATTACAG AGATAATACC 
AGGCTGAACT CAGAAGATTC TTCAGTGGAC TCCATTGCCT CGGTTGTGGT TCCCATAATA 
ATAGGCCTCT CTATCATAAT AGCATTCCTG TTCATCAATC AGAAGAAACA GTGGATACCA 
CTGCTTTGCT GGTATCGAAC ACCAACTAAG CCTTCTTCTT TAAATAATCA GCTGGTATCT 
GTGGACTGCA AGAAAGGAAC CCGGGTCCAG GTGGACAGTT CCCAGAGAAT GCTGAGAATC 
GCGGAACCAG ACGCGAGATT CAGTGGCTTC TACAGCATGC AGAAACAGAA CCACCTACAG 
GCAGACAATT TCTACCAAAC AGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRIM1 ( XM_006056855.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006056917.1 Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu13905
Clone ID Related Accession (Same CDS sequence)	XM_006056855.1
Accession Version	XM_006056855.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	cysteine-rich motor neuron 1 protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784779.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCA CAGCTCTAAT AGAAAGAGAT GAGAACTGGG ATGATGACCA ACTGCTTGGG 
TTTGAACCAT GCAATGAAAA CCTTGTCTCT GGCTGCAATC TCATCAATGG AAAATGTGAG 
TGTGACACCA TTCGAACCTG TAACAATCCC TTCGAGTTTC CAAGGAAGGA TATGTGCCTT 
TCAGCTTTAA AGAGGATTGA AGAAGAGAAG CCAGATTGCT CCAAGGCCCG CTGTGAAGTC 
CAGTTCTCTC CACGTTGTCC TGAAGACTCC GTTCTGATCG AGGGCTACGC GCCCCCCGGG 
GAGTGCTGCC CCCTCCCCAG CCGCTGTGTG TGTGACCCCG CGGGCTGCCT GCGGAAAGTC 
TGCCAGCCAG GATACCTGAA CATCCTTGTG TCCAAAGCCT CAGGGAAGCC GGGAGAGTGC 
TGTGACCTCT ATGAGTGCAA ACCAGTTTTC AGTGTGGACT GCAGCACCGT GGAGTGCCCC 
TCCTTTCAGC AAACCGTGTG CCCCCTGGAC AGCTACGAAA CCCAAGTCAG ACTCACAGCG 
GATGGCTGCT GCACTCTTCC AACAAGATGC GAGTGTCTCT CTGGCTTATG TGGTTTCCCC 
GTGTGTGAGG TGGGATCCAC TCCCCGCATA GTCTCTCGTG GAGATGGGAC ACCTGGAAAG 
TGCTGTGATG TCTTTGAATG TGTTAATGAA ACAAAGCCAG CCTGCGTGTT CAACAACGTG 
GAGTACAGTG ACGGAGACAT GTTTCGAATG GACAACTGTC GATTCTGTCG ATGCCAAGGG 
GGTGTTTCCA TCTGCTTCAC TGCCCAGTGT GGTGAGCTGA GCTGTGAGAG ATACTACATG 
CCCGAAGGGG AGTGCTGCCC TGTGTGTGAA GATCCTGTGT ATCCTTTGAA CAACCCCGCT 
GGCTGCTATG CCAATGGCCA GATCCACGCC CACGGGGACC GGTGGCGGGA AGACGACTGC 
ACGTTCTGCC AGTGCATCAA TGGGGAGCCC CACTGCGTGG CGACGGCCTG TGGACAGAGC 
TGCATGAACC CCGTCAAAGT GCCTGGGGAG TGCTGCCCTG TATGCGAAGA ACCAACCTAC 
ATCACAATTG ATCCACCTGC ATGTGGGGAG TTATCAAACT GTACCCTGAC AGAGAAGGAC 
TGCATTTATG GTTTCAAACT CGATCACAAT GGTTGTCGAA CCTGTCAGTG CAAAAACAGG 
GAGGAGCTCT GCACGGGCCT CAGGCGAGGC TGCGCCTTGA ACTGCCCCTT CGGCTTCCTA 
ACCGACGCCT ACGGCTGTGA CGCCTGTCAG TGCCGCCCGC GGCCCCGGAA GTGCAGACCC 
ACAGCCTGCG AAAAGCATTG TCCGTTCGGA TACCTGAAGA ATAAGCACGG CTGTGACATC 
TGTCGCTGTA AGAAATGCCC AGAGCTCCGG TGCAGTAAGA TCTGCTCCCT GGGTTTCCAG 
AAGGACAGTC ATGGCTGTCT CATCTGCAAG TGCAGAGAGG TCTCTGCCTC CGCCGGGCCA 
CCCGTCCTGT CAGGCACGTG TCTATCCATG GATGGCCATC ATCATAAAAA TGAGGAGAGC 
TGGCATGATG GGTGTCGGGA ATGCTACTGC CACAGTGGAC GGGAGATGTG CGCACTGATC 
ACCTGCCCCG TGCCTGCCTG TGGCAACCCC ACCATTCACC CGGGACGGTG CTGCCCATCA 
TGTTCAGATG ACTTCGTGGT GCAGAAGCCA GAGCTCAGCA CGCCCTCCAT CTGCCACGCC 
CCCGGGGGAG AGTACTTTGT GGAGGGAGAA ACGTGGCACA TTGACACCTG CACGCAATGC 
ACGTGCCACA GCGGGCGGGT GCTGTGTGAG ACGGAGGTGT GCCCGCCGCT GCTCTGCCAG 
AACCCCACAC GCACCCAGGA CTCCTGCTGC CCACAGTGCC CAGATGAACC TCTCCAGCCT 
TCCTCATCCC ACAATGACAG TGTGCCTAGT TACTGCAAAA ACGATGAAGG GGATATATTC 
CTGGCAGCTG AGTCCTGGAA ACCCGACGTG TGTACCAGCT GCGTGTGCAT GGATAGCGTC 
ATTAGCTGTT ACTCTGAGTC TTGCCCTTCT GTATCCTGTG AAAGACCTGT GTTGAGAAAA 
GGCCAGTGTT GTCCCTACTG CATAGAGGAC ACAATCCCAA AGAAGGTGGT GTGCCACTTC 
AGCGGGAAGG CCTACGCTGA CGAGGAGCGC TGGGACATCG ACAGCTGCAC GCACTGCTAC 
TGCCTGCAGG GCCAGACCCT CTGCTCCACC GTCAGCTGCC CGCCGCTGCC CTGCGTCGAG 
CCCATCAACG TGGAAGGCAG CTGCTGCCCC ATGTGCCCAG AAATGTATGT CCCAGAACCA 
ACCAATATAC CCATTGAGAA GACAAATCAT CGGGAGATCG ACATGGAGGT CCCCCTGTGG 
CCCACACCTA GTGAAAATGA CATTATCCAT CTCCCCAGAG ACATGGGTCA CCCCCAGATA 
GATTACAGAG ATAATACCAG GCTGAACTCA GAAGATTCTT CAGTGGACTC CATTGCCTCG 
GTTGTGGTTC CCATAATAAT AGGCCTCTCT ATCATAATAG CATTCCTGTT CATCAATCAG 
AAGAAACAGT GGATACCACT GCTTTGCTGG TATCGAACAC CAACTAAGCC TTCTTCTTTA 
AATAATCAGC TGGTATCTGT GGACTGCAAG AAAGGAACCC GGGTCCAGGT GGACAGTTCC 
CAGAGAATGC TGAGAATCGC GGAACCAGAC GCGAGATTCA GTGGCTTCTA CAGCATGCAG 
AAACAGAACC ACCTACAGGC AGACAATTTC TACCAAACAG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006056917.1
CDS	2381..5185
Translation	MKITALIERDENWDDDQLLGFEPCNENLVSGCNLINGKCECDTIRTCNNPFEFPRKDMCLSALKRIEEEKPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCDPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPSFQQTVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPVCEVGSTPRIVSRGDGTPGKCCDVFECVNETKPACVFNNVEYSDGDMFRMDNCRFCRCQGGVSICFTAQCGELSCERYYMPEGECCPVCEDPVYPLNNPAGCYANGQIHAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLTEKDCIYGFKLDHNGCRTCQCKNREELCTGLRRGCALNCPFGFLTDAYGCDACQCRPRPRKCRPTACEKHCPFGYLKNKHGCDICRCKKCPELRCSKICSLGFQKDSHGCLICKCREVSASAGPPVLSGTCLSMDGHHHKNEESWHDGCRECYCHSGREMCALITCPVPACGNPTIHPGRCCPSCSDDFVVQKPELSTPSICHAPGGEYFVEGETWHIDTCTQCTCHSGRVLCETEVCPPLLCQNPTRTQDSCCPQCPDEPLQPSSSHNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCYSESCPSVSCERPVLRKGQCCPYCIEDTIPKKVVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHREIDMEVPLWPTPSENDIIHLPRDMGHPQIDYRDNTRLNSEDSSVDSIASVVVPIIIGLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGTRVQVDSSQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_006056855.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006056855.1

ORF Insert Sequence:

ATGAAGATCA CAGCTCTAAT AGAAAGAGAT GAGAACTGGG ATGATGACCA ACTGCTTGGG 
TTTGAACCAT GCAATGAAAA CCTTGTCTCT GGCTGCAATC TCATCAATGG AAAATGTGAG 
TGTGACACCA TTCGAACCTG TAACAATCCC TTCGAGTTTC CAAGGAAGGA TATGTGCCTT 
TCAGCTTTAA AGAGGATTGA AGAAGAGAAG CCAGATTGCT CCAAGGCCCG CTGTGAAGTC 
CAGTTCTCTC CACGTTGTCC TGAAGACTCC GTTCTGATCG AGGGCTACGC GCCCCCCGGG 
GAGTGCTGCC CCCTCCCCAG CCGCTGTGTG TGTGACCCCG CGGGCTGCCT GCGGAAAGTC 
TGCCAGCCAG GATACCTGAA CATCCTTGTG TCCAAAGCCT CAGGGAAGCC GGGAGAGTGC 
TGTGACCTCT ATGAGTGCAA ACCAGTTTTC AGTGTGGACT GCAGCACCGT GGAGTGCCCC 
TCCTTTCAGC AAACCGTGTG CCCCCTGGAC AGCTACGAAA CCCAAGTCAG ACTCACAGCG 
GATGGCTGCT GCACTCTTCC AACAAGATGC GAGTGTCTCT CTGGCTTATG TGGTTTCCCC 
GTGTGTGAGG TGGGATCCAC TCCCCGCATA GTCTCTCGTG GAGATGGGAC ACCTGGAAAG 
TGCTGTGATG TCTTTGAATG TGTTAATGAA ACAAAGCCAG CCTGCGTGTT CAACAACGTG 
GAGTACAGTG ACGGAGACAT GTTTCGAATG GACAACTGTC GATTCTGTCG ATGCCAAGGG 
GGTGTTTCCA TCTGCTTCAC TGCCCAGTGT GGTGAGCTGA GCTGTGAGAG ATACTACATG 
CCCGAAGGGG AGTGCTGCCC TGTGTGTGAA GATCCTGTGT ATCCTTTGAA CAACCCCGCT 
GGCTGCTATG CCAATGGCCA GATCCACGCC CACGGGGACC GGTGGCGGGA AGACGACTGC 
ACGTTCTGCC AGTGCATCAA TGGGGAGCCC CACTGCGTGG CGACGGCCTG TGGACAGAGC 
TGCATGAACC CCGTCAAAGT GCCTGGGGAG TGCTGCCCTG TATGCGAAGA ACCAACCTAC 
ATCACAATTG ATCCACCTGC ATGTGGGGAG TTATCAAACT GTACCCTGAC AGAGAAGGAC 
TGCATTTATG GTTTCAAACT CGATCACAAT GGTTGTCGAA CCTGTCAGTG CAAAAACAGG 
GAGGAGCTCT GCACGGGCCT CAGGCGAGGC TGCGCCTTGA ACTGCCCCTT CGGCTTCCTA 
ACCGACGCCT ACGGCTGTGA CGCCTGTCAG TGCCGCCCGC GGCCCCGGAA GTGCAGACCC 
ACAGCCTGCG AAAAGCATTG TCCGTTCGGA TACCTGAAGA ATAAGCACGG CTGTGACATC 
TGTCGCTGTA AGAAATGCCC AGAGCTCCGG TGCAGTAAGA TCTGCTCCCT GGGTTTCCAG 
AAGGACAGTC ATGGCTGTCT CATCTGCAAG TGCAGAGAGG TCTCTGCCTC CGCCGGGCCA 
CCCGTCCTGT CAGGCACGTG TCTATCCATG GATGGCCATC ATCATAAAAA TGAGGAGAGC 
TGGCATGATG GGTGTCGGGA ATGCTACTGC CACAGTGGAC GGGAGATGTG CGCACTGATC 
ACCTGCCCCG TGCCTGCCTG TGGCAACCCC ACCATTCACC CGGGACGGTG CTGCCCATCA 
TGTTCAGATG ACTTCGTGGT GCAGAAGCCA GAGCTCAGCA CGCCCTCCAT CTGCCACGCC 
CCCGGGGGAG AGTACTTTGT GGAGGGAGAA ACGTGGCACA TTGACACCTG CACGCAATGC 
ACGTGCCACA GCGGGCGGGT GCTGTGTGAG ACGGAGGTGT GCCCGCCGCT GCTCTGCCAG 
AACCCCACAC GCACCCAGGA CTCCTGCTGC CCACAGTGCC CAGATGAACC TCTCCAGCCT 
TCCTCATCCC ACAATGACAG TGTGCCTAGT TACTGCAAAA ACGATGAAGG GGATATATTC 
CTGGCAGCTG AGTCCTGGAA ACCCGACGTG TGTACCAGCT GCGTGTGCAT GGATAGCGTC 
ATTAGCTGTT ACTCTGAGTC TTGCCCTTCT GTATCCTGTG AAAGACCTGT GTTGAGAAAA 
GGCCAGTGTT GTCCCTACTG CATAGAGGAC ACAATCCCAA AGAAGGTGGT GTGCCACTTC 
AGCGGGAAGG CCTACGCTGA CGAGGAGCGC TGGGACATCG ACAGCTGCAC GCACTGCTAC 
TGCCTGCAGG GCCAGACCCT CTGCTCCACC GTCAGCTGCC CGCCGCTGCC CTGCGTCGAG 
CCCATCAACG TGGAAGGCAG CTGCTGCCCC ATGTGCCCAG AAATGTATGT CCCAGAACCA 
ACCAATATAC CCATTGAGAA GACAAATCAT CGGGAGATCG ACATGGAGGT CCCCCTGTGG 
CCCACACCTA GTGAAAATGA CATTATCCAT CTCCCCAGAG ACATGGGTCA CCCCCAGATA 
GATTACAGAG ATAATACCAG GCTGAACTCA GAAGATTCTT CAGTGGACTC CATTGCCTCG 
GTTGTGGTTC CCATAATAAT AGGCCTCTCT ATCATAATAG CATTCCTGTT CATCAATCAG 
AAGAAACAGT GGATACCACT GCTTTGCTGG TATCGAACAC CAACTAAGCC TTCTTCTTTA 
AATAATCAGC TGGTATCTGT GGACTGCAAG AAAGGAACCC GGGTCCAGGT GGACAGTTCC 
CAGAGAATGC TGAGAATCGC GGAACCAGAC GCGAGATTCA GTGGCTTCTA CAGCATGCAG 
AAACAGAACC ACCTACAGGC AGACAATTTC TACCAAACAG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRIM1 ( XM_006056853.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006056915.1 Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (CRIM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu45932
Clone ID Related Accession (Same CDS sequence)	XM_006056853.2
Accession Version	XM_006056853.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cysteine-rich motor neuron 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006056853.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCT GGTCTCGCTG 
CTGGGGCTGT TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAAGA GCCCAAGAGC TGCCCCGGGA GCATCGTGCA GGGCTTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGA AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GAAGAGAAGC CAGATTGCTC CAAGGCCCGC 
TGTGAAGTCC AGTTCTCTCC ACGTTGTCCT GAAGACTCCG TTCTGATCGA GGGCTACGCG 
CCCCCCGGGG AGTGCTGCCC CCTCCCCAGC CGCTGTGTGT GTGACCCCGC GGGCTGCCTG 
CGGAAAGTCT GCCAGCCAGG ATACCTGAAC ATCCTTGTGT CCAAAGCCTC AGGGAAGCCG 
GGAGAGTGCT GTGACCTCTA TGAGTGCAAA CCAGTTTTCA GTGTGGACTG CAGCACCGTG 
GAGTGCCCCT CCTTTCAGCA AACCGTGTGC CCCCTGGACA GCTACGAAAC CCAAGTCAGA 
CTCACAGCGG ATGGCTGCTG CACTCTTCCA ACAAGATGCG AGTGTCTCTC TGGCTTATGT 
GGTTTCCCCG TGTGTGAGGT GGGATCCACT CCCCGCATAG TCTCTCGTGG AGATGGGACA 
CCTGGAAAGT GCTGTGATGT CTTTGAATGT GTTAATGAAA CAAAGCCAGC CTGCGTGTTC 
AACAACGTGG AGTACAGTGA CGGAGACATG TTTCGAATGG ACAACTGTCG ATTCTGTCGA 
TGCCAAGGGG GTGTTTCCAT CTGCTTCACT GCCCAGTGTG GTGAGCTGAG CTGTGAGAGA 
TACTACATGC CCGAAGGGGA GTGCTGCCCT GTGTGTGAAG ATCCTGTGTA TCCTTTGAAC 
AACCCCGCTG GCTGCTATGC CAATGGCCAG ATCCACGCCC ACGGGGACCG GTGGCGGGAA 
GACGACTGCA CGTTCTGCCA GTGCATCAAT GGGGAGCCCC ACTGCGTGGC GACGGCCTGT 
GGACAGAGCT GCATGAACCC CGTCAAAGTG CCTGGGGAGT GCTGCCCTGT ATGCGAAGAA 
CCAACCTACA TCACAATTGA TCCACCTGCA TGTGGGGAGT TATCAAACTG TACCCTGACA 
GAGAAGGACT GCATTTATGG TTTCAAACTC GATCACAATG GTTGTCGAAC CTGTCAGTGC 
AAAAACAGGG AGGAGCTCTG CACGGGCCTC AGGCGAGGCT GCGCCTTGAA CTGCCCCTTC 
GGCTTCCTAA CCGACGCCTA CGGCTGTGAC GCCTGTCAGT GCCGCCCGCG CCCCCGGAAG 
TGCAGACCCA CAGCCTGCGA AAAGCATTGT CCGTTCGGAT ACCTGAAGAA TAAGCACGGC 
TGTGACATCT GTCGCTGTAA GAAATGCCCA GAGCTCCGGT GCAGTAAGAT CTGCTCCCTG 
GGTTTCCAGA AGGACAGTCA TGGCTGTCTC ATCTGCAAGT GCAGAGAGGT CTCTGCCTCC 
GCCGGGCCAC CCGTCCTGTC AGGCACGTGT CTATCCATGG ATGGCCATCA TCATAAAAAT 
GAGGAGAGCT GGCATGATGG GTGTCGGGAA TGCTACTGCC ACAGTGGACG GGAGATGTGC 
GCACTGATCA CCTGCCCCGT GCCTGCCTGT GGCAACCCCA CCATTCACCC GGGACGGTGC 
TGCCCATCAT GTTCAGATGA CTTCGTGGTG CAGAAGCCAG AGCTCAGCAC GCCCTCCATC 
TGCCACGCCC CCGGGGGAGA GTACTTTGTG GAGGGAGAAA CATGGCACAT TGACACCTGC 
ACGCAATGCA CGTGCCACAG CGGGCGGGTG CTGTGTGAGA CGGAGGTGTG CCCGCCGCTG 
CTCTGCCAGA ACCCCACACG CACCCAGGAC TCCTGCTGCC CACAGTGCCC AGATGAACCT 
CTCCAGCCTT CCTCATCCCA CAATGACAGT GTGCCTAGTT ACTGCAAAAA CGATGAAGGG 
GATATATTCC TGGCAGCTGA GTCCTGGAAA CCCGACGTGT GTACCAGCTG CGTGTGCATG 
GATAGCGTCA TTAGCTGTTA CTCTGAGTCT TGCCCTTCTG TATCCTGTGA AAGACCTGTG 
TTGAGAAAAG GCCAGTGTTG TCCCTACTGC ATAGAGGACA CAATCCCAAA GAAGGTGGTG 
TGCCACTTCA GCGGGAAGGC CTACGCTGAC GAGGAGCGCT GGGACATCGA CAGCTGCACG 
CACTGCTACT GCCTGCAGGG CCAGACCCTC TGCTCCACCG TCAGCTGCCC GCCGCTGCCC 
TGCGTCGAGC CCATCAACGT GGAAGGCAGC TGCTGCCCCA TGTGCCCAGA AATGTATGTC 
CCAGAACCAA CCAATATACC CATTGAGAAG ACAAATCATC GGGAGATCGA CATGGAGGTC 
CCCCTGTGGC CCACACCTAG TGAAAATGAC ATTATCCATC TCCCCAGAGA CATGGGTCAC 
CCCCAGATAG ATTACAGAGA TAATACCAGG CTGAACTCAG AAGATTCTTC AGTGGACTCC 
ATTGCCTCGG TTGTGGTTCC CATAATAATA GGCCTCTCTA TCATAATAGC ATTCCTGTTC 
ATCAATCAGA AGAAACAGTG GATACCACTG CTTTGCTGGT ATCGAACACC AACTAAGCCT 
TCTTCTTTAA ATAATCAGCT GGTATCTGTG GACTGCAAGA AAGGAACCCG GGTCCAGGTG 
GACAGTTCCC AGAGAATGCT GAGAATCGCG GAACCAGACG CGAGATTCAG TGGCTTCTAC 
AGCATGCAGA AACAGAACCA CCTACAGGCA GACAATTTCT ACCAAACAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006056915.1
CDS	534..3467
Translation	MYLVAGGRGLAGCGHLLVSLLGLLLLLARSGTRALVCLPCDESKCEEPKSCPGSIVQGFCGCCYMCARQRNESCGGAYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEEEKPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCDPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPSFQQTVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPVCEVGSTPRIVSRGDGTPGKCCDVFECVNETKPACVFNNVEYSDGDMFRMDNCRFCRCQGGVSICFTAQCGELSCERYYMPEGECCPVCEDPVYPLNNPAGCYANGQIHAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLTEKDCIYGFKLDHNGCRTCQCKNREELCTGLRRGCALNCPFGFLTDAYGCDACQCRPRPRKCRPTACEKHCPFGYLKNKHGCDICRCKKCPELRCSKICSLGFQKDSHGCLICKCREVSASAGPPVLSGTCLSMDGHHHKNEESWHDGCRECYCHSGREMCALITCPVPACGNPTIHPGRCCPSCSDDFVVQKPELSTPSICHAPGGEYFVEGETWHIDTCTQCTCHSGRVLCETEVCPPLLCQNPTRTQDSCCPQCPDEPLQPSSSHNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCYSESCPSVSCERPVLRKGQCCPYCIEDTIPKKVVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHREIDMEVPLWPTPSENDIIHLPRDMGHPQIDYRDNTRLNSEDSSVDSIASVVVPIIIGLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGTRVQVDSSQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_006056853.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (CRIM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006056853.2

ORF Insert Sequence:

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCT GGTCTCGCTG 
CTGGGGCTGT TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAAGA GCCCAAGAGC TGCCCCGGGA GCATCGTGCA GGGCTTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGA AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GAAGAGAAGC CAGATTGCTC CAAGGCCCGC 
TGTGAAGTCC AGTTCTCTCC ACGTTGTCCT GAAGACTCCG TTCTGATCGA GGGCTACGCG 
CCCCCCGGGG AGTGCTGCCC CCTCCCCAGC CGCTGTGTGT GTGACCCCGC GGGCTGCCTG 
CGGAAAGTCT GCCAGCCAGG ATACCTGAAC ATCCTTGTGT CCAAAGCCTC AGGGAAGCCG 
GGAGAGTGCT GTGACCTCTA TGAGTGCAAA CCAGTTTTCA GTGTGGACTG CAGCACCGTG 
GAGTGCCCCT CCTTTCAGCA AACCGTGTGC CCCCTGGACA GCTACGAAAC CCAAGTCAGA 
CTCACAGCGG ATGGCTGCTG CACTCTTCCA ACAAGATGCG AGTGTCTCTC TGGCTTATGT 
GGTTTCCCCG TGTGTGAGGT GGGATCCACT CCCCGCATAG TCTCTCGTGG AGATGGGACA 
CCTGGAAAGT GCTGTGATGT CTTTGAATGT GTTAATGAAA CAAAGCCAGC CTGCGTGTTC 
AACAACGTGG AGTACAGTGA CGGAGACATG TTTCGAATGG ACAACTGTCG ATTCTGTCGA 
TGCCAAGGGG GTGTTTCCAT CTGCTTCACT GCCCAGTGTG GTGAGCTGAG CTGTGAGAGA 
TACTACATGC CCGAAGGGGA GTGCTGCCCT GTGTGTGAAG ATCCTGTGTA TCCTTTGAAC 
AACCCCGCTG GCTGCTATGC CAATGGCCAG ATCCACGCCC ACGGGGACCG GTGGCGGGAA 
GACGACTGCA CGTTCTGCCA GTGCATCAAT GGGGAGCCCC ACTGCGTGGC GACGGCCTGT 
GGACAGAGCT GCATGAACCC CGTCAAAGTG CCTGGGGAGT GCTGCCCTGT ATGCGAAGAA 
CCAACCTACA TCACAATTGA TCCACCTGCA TGTGGGGAGT TATCAAACTG TACCCTGACA 
GAGAAGGACT GCATTTATGG TTTCAAACTC GATCACAATG GTTGTCGAAC CTGTCAGTGC 
AAAAACAGGG AGGAGCTCTG CACGGGCCTC AGGCGAGGCT GCGCCTTGAA CTGCCCCTTC 
GGCTTCCTAA CCGACGCCTA CGGCTGTGAC GCCTGTCAGT GCCGCCCGCG CCCCCGGAAG 
TGCAGACCCA CAGCCTGCGA AAAGCATTGT CCGTTCGGAT ACCTGAAGAA TAAGCACGGC 
TGTGACATCT GTCGCTGTAA GAAATGCCCA GAGCTCCGGT GCAGTAAGAT CTGCTCCCTG 
GGTTTCCAGA AGGACAGTCA TGGCTGTCTC ATCTGCAAGT GCAGAGAGGT CTCTGCCTCC 
GCCGGGCCAC CCGTCCTGTC AGGCACGTGT CTATCCATGG ATGGCCATCA TCATAAAAAT 
GAGGAGAGCT GGCATGATGG GTGTCGGGAA TGCTACTGCC ACAGTGGACG GGAGATGTGC 
GCACTGATCA CCTGCCCCGT GCCTGCCTGT GGCAACCCCA CCATTCACCC GGGACGGTGC 
TGCCCATCAT GTTCAGATGA CTTCGTGGTG CAGAAGCCAG AGCTCAGCAC GCCCTCCATC 
TGCCACGCCC CCGGGGGAGA GTACTTTGTG GAGGGAGAAA CATGGCACAT TGACACCTGC 
ACGCAATGCA CGTGCCACAG CGGGCGGGTG CTGTGTGAGA CGGAGGTGTG CCCGCCGCTG 
CTCTGCCAGA ACCCCACACG CACCCAGGAC TCCTGCTGCC CACAGTGCCC AGATGAACCT 
CTCCAGCCTT CCTCATCCCA CAATGACAGT GTGCCTAGTT ACTGCAAAAA CGATGAAGGG 
GATATATTCC TGGCAGCTGA GTCCTGGAAA CCCGACGTGT GTACCAGCTG CGTGTGCATG 
GATAGCGTCA TTAGCTGTTA CTCTGAGTCT TGCCCTTCTG TATCCTGTGA AAGACCTGTG 
TTGAGAAAAG GCCAGTGTTG TCCCTACTGC ATAGAGGACA CAATCCCAAA GAAGGTGGTG 
TGCCACTTCA GCGGGAAGGC CTACGCTGAC GAGGAGCGCT GGGACATCGA CAGCTGCACG 
CACTGCTACT GCCTGCAGGG CCAGACCCTC TGCTCCACCG TCAGCTGCCC GCCGCTGCCC 
TGCGTCGAGC CCATCAACGT GGAAGGCAGC TGCTGCCCCA TGTGCCCAGA AATGTATGTC 
CCAGAACCAA CCAATATACC CATTGAGAAG ACAAATCATC GGGAGATCGA CATGGAGGTC 
CCCCTGTGGC CCACACCTAG TGAAAATGAC ATTATCCATC TCCCCAGAGA CATGGGTCAC 
CCCCAGATAG ATTACAGAGA TAATACCAGG CTGAACTCAG AAGATTCTTC AGTGGACTCC 
ATTGCCTCGG TTGTGGTTCC CATAATAATA GGCCTCTCTA TCATAATAGC ATTCCTGTTC 
ATCAATCAGA AGAAACAGTG GATACCACTG CTTTGCTGGT ATCGAACACC AACTAAGCCT 
TCTTCTTTAA ATAATCAGCT GGTATCTGTG GACTGCAAGA AAGGAACCCG GGTCCAGGTG 
GACAGTTCCC AGAGAATGCT GAGAATCGCG GAACCAGACG CGAGATTCAG TGGCTTCTAC 
AGCATGCAGA AACAGAACCA CCTACAGGCA GACAATTTCT ACCAAACAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRIM1 ( XM_006056855.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006056917.1 Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (CRIM1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu45933
Clone ID Related Accession (Same CDS sequence)	XM_006056855.2
Accession Version	XM_006056855.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cysteine-rich motor neuron 1 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006056855.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCA CAGCTCTAAT AGAAAGAGAT GAGAACTGGG ATGATGACCA ACTGCTTGGG 
TTTGAACCAT GCAATGAAAA CCTTGTCTCT GGCTGCAATC TCATCAATGG AAAATGTGAG 
TGTGACACCA TTCGAACCTG TAACAATCCC TTCGAGTTTC CAAGGAAGGA TATGTGCCTT 
TCAGCTTTAA AGAGGATTGA AGAAGAGAAG CCAGATTGCT CCAAGGCCCG CTGTGAAGTC 
CAGTTCTCTC CACGTTGTCC TGAAGACTCC GTTCTGATCG AGGGCTACGC GCCCCCCGGG 
GAGTGCTGCC CCCTCCCCAG CCGCTGTGTG TGTGACCCCG CGGGCTGCCT GCGGAAAGTC 
TGCCAGCCAG GATACCTGAA CATCCTTGTG TCCAAAGCCT CAGGGAAGCC GGGAGAGTGC 
TGTGACCTCT ATGAGTGCAA ACCAGTTTTC AGTGTGGACT GCAGCACCGT GGAGTGCCCC 
TCCTTTCAGC AAACCGTGTG CCCCCTGGAC AGCTACGAAA CCCAAGTCAG ACTCACAGCG 
GATGGCTGCT GCACTCTTCC AACAAGATGC GAGTGTCTCT CTGGCTTATG TGGTTTCCCC 
GTGTGTGAGG TGGGATCCAC TCCCCGCATA GTCTCTCGTG GAGATGGGAC ACCTGGAAAG 
TGCTGTGATG TCTTTGAATG TGTTAATGAA ACAAAGCCAG CCTGCGTGTT CAACAACGTG 
GAGTACAGTG ACGGAGACAT GTTTCGAATG GACAACTGTC GATTCTGTCG ATGCCAAGGG 
GGTGTTTCCA TCTGCTTCAC TGCCCAGTGT GGTGAGCTGA GCTGTGAGAG ATACTACATG 
CCCGAAGGGG AGTGCTGCCC TGTGTGTGAA GATCCTGTGT ATCCTTTGAA CAACCCCGCT 
GGCTGCTATG CCAATGGCCA GATCCACGCC CACGGGGACC GGTGGCGGGA AGACGACTGC 
ACGTTCTGCC AGTGCATCAA TGGGGAGCCC CACTGCGTGG CGACGGCCTG TGGACAGAGC 
TGCATGAACC CCGTCAAAGT GCCTGGGGAG TGCTGCCCTG TATGCGAAGA ACCAACCTAC 
ATCACAATTG ATCCACCTGC ATGTGGGGAG TTATCAAACT GTACCCTGAC AGAGAAGGAC 
TGCATTTATG GTTTCAAACT CGATCACAAT GGTTGTCGAA CCTGTCAGTG CAAAAACAGG 
GAGGAGCTCT GCACGGGCCT CAGGCGAGGC TGCGCCTTGA ACTGCCCCTT CGGCTTCCTA 
ACCGACGCCT ACGGCTGTGA CGCCTGTCAG TGCCGCCCGC GCCCCCGGAA GTGCAGACCC 
ACAGCCTGCG AAAAGCATTG TCCGTTCGGA TACCTGAAGA ATAAGCACGG CTGTGACATC 
TGTCGCTGTA AGAAATGCCC AGAGCTCCGG TGCAGTAAGA TCTGCTCCCT GGGTTTCCAG 
AAGGACAGTC ATGGCTGTCT CATCTGCAAG TGCAGAGAGG TCTCTGCCTC CGCCGGGCCA 
CCCGTCCTGT CAGGCACGTG TCTATCCATG GATGGCCATC ATCATAAAAA TGAGGAGAGC 
TGGCATGATG GGTGTCGGGA ATGCTACTGC CACAGTGGAC GGGAGATGTG CGCACTGATC 
ACCTGCCCCG TGCCTGCCTG TGGCAACCCC ACCATTCACC CGGGACGGTG CTGCCCATCA 
TGTTCAGATG ACTTCGTGGT GCAGAAGCCA GAGCTCAGCA CGCCCTCCAT CTGCCACGCC 
CCCGGGGGAG AGTACTTTGT GGAGGGAGAA ACATGGCACA TTGACACCTG CACGCAATGC 
ACGTGCCACA GCGGGCGGGT GCTGTGTGAG ACGGAGGTGT GCCCGCCGCT GCTCTGCCAG 
AACCCCACAC GCACCCAGGA CTCCTGCTGC CCACAGTGCC CAGATGAACC TCTCCAGCCT 
TCCTCATCCC ACAATGACAG TGTGCCTAGT TACTGCAAAA ACGATGAAGG GGATATATTC 
CTGGCAGCTG AGTCCTGGAA ACCCGACGTG TGTACCAGCT GCGTGTGCAT GGATAGCGTC 
ATTAGCTGTT ACTCTGAGTC TTGCCCTTCT GTATCCTGTG AAAGACCTGT GTTGAGAAAA 
GGCCAGTGTT GTCCCTACTG CATAGAGGAC ACAATCCCAA AGAAGGTGGT GTGCCACTTC 
AGCGGGAAGG CCTACGCTGA CGAGGAGCGC TGGGACATCG ACAGCTGCAC GCACTGCTAC 
TGCCTGCAGG GCCAGACCCT CTGCTCCACC GTCAGCTGCC CGCCGCTGCC CTGCGTCGAG 
CCCATCAACG TGGAAGGCAG CTGCTGCCCC ATGTGCCCAG AAATGTATGT CCCAGAACCA 
ACCAATATAC CCATTGAGAA GACAAATCAT CGGGAGATCG ACATGGAGGT CCCCCTGTGG 
CCCACACCTA GTGAAAATGA CATTATCCAT CTCCCCAGAG ACATGGGTCA CCCCCAGATA 
GATTACAGAG ATAATACCAG GCTGAACTCA GAAGATTCTT CAGTGGACTC CATTGCCTCG 
GTTGTGGTTC CCATAATAAT AGGCCTCTCT ATCATAATAG CATTCCTGTT CATCAATCAG 
AAGAAACAGT GGATACCACT GCTTTGCTGG TATCGAACAC CAACTAAGCC TTCTTCTTTA 
AATAATCAGC TGGTATCTGT GGACTGCAAG AAAGGAACCC GGGTCCAGGT GGACAGTTCC 
CAGAGAATGC TGAGAATCGC GGAACCAGAC GCGAGATTCA GTGGCTTCTA CAGCATGCAG 
AAACAGAACC ACCTACAGGC AGACAATTTC TACCAAACAG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006056917.1
CDS	1910..4714
Translation	MKITALIERDENWDDDQLLGFEPCNENLVSGCNLINGKCECDTIRTCNNPFEFPRKDMCLSALKRIEEEKPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCDPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPSFQQTVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPVCEVGSTPRIVSRGDGTPGKCCDVFECVNETKPACVFNNVEYSDGDMFRMDNCRFCRCQGGVSICFTAQCGELSCERYYMPEGECCPVCEDPVYPLNNPAGCYANGQIHAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLTEKDCIYGFKLDHNGCRTCQCKNREELCTGLRRGCALNCPFGFLTDAYGCDACQCRPRPRKCRPTACEKHCPFGYLKNKHGCDICRCKKCPELRCSKICSLGFQKDSHGCLICKCREVSASAGPPVLSGTCLSMDGHHHKNEESWHDGCRECYCHSGREMCALITCPVPACGNPTIHPGRCCPSCSDDFVVQKPELSTPSICHAPGGEYFVEGETWHIDTCTQCTCHSGRVLCETEVCPPLLCQNPTRTQDSCCPQCPDEPLQPSSSHNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCYSESCPSVSCERPVLRKGQCCPYCIEDTIPKKVVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHREIDMEVPLWPTPSENDIIHLPRDMGHPQIDYRDNTRLNSEDSSVDSIASVVVPIIIGLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGTRVQVDSSQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_006056855.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (CRIM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006056855.2

ORF Insert Sequence:

ATGAAGATCA CAGCTCTAAT AGAAAGAGAT GAGAACTGGG ATGATGACCA ACTGCTTGGG 
TTTGAACCAT GCAATGAAAA CCTTGTCTCT GGCTGCAATC TCATCAATGG AAAATGTGAG 
TGTGACACCA TTCGAACCTG TAACAATCCC TTCGAGTTTC CAAGGAAGGA TATGTGCCTT 
TCAGCTTTAA AGAGGATTGA AGAAGAGAAG CCAGATTGCT CCAAGGCCCG CTGTGAAGTC 
CAGTTCTCTC CACGTTGTCC TGAAGACTCC GTTCTGATCG AGGGCTACGC GCCCCCCGGG 
GAGTGCTGCC CCCTCCCCAG CCGCTGTGTG TGTGACCCCG CGGGCTGCCT GCGGAAAGTC 
TGCCAGCCAG GATACCTGAA CATCCTTGTG TCCAAAGCCT CAGGGAAGCC GGGAGAGTGC 
TGTGACCTCT ATGAGTGCAA ACCAGTTTTC AGTGTGGACT GCAGCACCGT GGAGTGCCCC 
TCCTTTCAGC AAACCGTGTG CCCCCTGGAC AGCTACGAAA CCCAAGTCAG ACTCACAGCG 
GATGGCTGCT GCACTCTTCC AACAAGATGC GAGTGTCTCT CTGGCTTATG TGGTTTCCCC 
GTGTGTGAGG TGGGATCCAC TCCCCGCATA GTCTCTCGTG GAGATGGGAC ACCTGGAAAG 
TGCTGTGATG TCTTTGAATG TGTTAATGAA ACAAAGCCAG CCTGCGTGTT CAACAACGTG 
GAGTACAGTG ACGGAGACAT GTTTCGAATG GACAACTGTC GATTCTGTCG ATGCCAAGGG 
GGTGTTTCCA TCTGCTTCAC TGCCCAGTGT GGTGAGCTGA GCTGTGAGAG ATACTACATG 
CCCGAAGGGG AGTGCTGCCC TGTGTGTGAA GATCCTGTGT ATCCTTTGAA CAACCCCGCT 
GGCTGCTATG CCAATGGCCA GATCCACGCC CACGGGGACC GGTGGCGGGA AGACGACTGC 
ACGTTCTGCC AGTGCATCAA TGGGGAGCCC CACTGCGTGG CGACGGCCTG TGGACAGAGC 
TGCATGAACC CCGTCAAAGT GCCTGGGGAG TGCTGCCCTG TATGCGAAGA ACCAACCTAC 
ATCACAATTG ATCCACCTGC ATGTGGGGAG TTATCAAACT GTACCCTGAC AGAGAAGGAC 
TGCATTTATG GTTTCAAACT CGATCACAAT GGTTGTCGAA CCTGTCAGTG CAAAAACAGG 
GAGGAGCTCT GCACGGGCCT CAGGCGAGGC TGCGCCTTGA ACTGCCCCTT CGGCTTCCTA 
ACCGACGCCT ACGGCTGTGA CGCCTGTCAG TGCCGCCCGC GCCCCCGGAA GTGCAGACCC 
ACAGCCTGCG AAAAGCATTG TCCGTTCGGA TACCTGAAGA ATAAGCACGG CTGTGACATC 
TGTCGCTGTA AGAAATGCCC AGAGCTCCGG TGCAGTAAGA TCTGCTCCCT GGGTTTCCAG 
AAGGACAGTC ATGGCTGTCT CATCTGCAAG TGCAGAGAGG TCTCTGCCTC CGCCGGGCCA 
CCCGTCCTGT CAGGCACGTG TCTATCCATG GATGGCCATC ATCATAAAAA TGAGGAGAGC 
TGGCATGATG GGTGTCGGGA ATGCTACTGC CACAGTGGAC GGGAGATGTG CGCACTGATC 
ACCTGCCCCG TGCCTGCCTG TGGCAACCCC ACCATTCACC CGGGACGGTG CTGCCCATCA 
TGTTCAGATG ACTTCGTGGT GCAGAAGCCA GAGCTCAGCA CGCCCTCCAT CTGCCACGCC 
CCCGGGGGAG AGTACTTTGT GGAGGGAGAA ACATGGCACA TTGACACCTG CACGCAATGC 
ACGTGCCACA GCGGGCGGGT GCTGTGTGAG ACGGAGGTGT GCCCGCCGCT GCTCTGCCAG 
AACCCCACAC GCACCCAGGA CTCCTGCTGC CCACAGTGCC CAGATGAACC TCTCCAGCCT 
TCCTCATCCC ACAATGACAG TGTGCCTAGT TACTGCAAAA ACGATGAAGG GGATATATTC 
CTGGCAGCTG AGTCCTGGAA ACCCGACGTG TGTACCAGCT GCGTGTGCAT GGATAGCGTC 
ATTAGCTGTT ACTCTGAGTC TTGCCCTTCT GTATCCTGTG AAAGACCTGT GTTGAGAAAA 
GGCCAGTGTT GTCCCTACTG CATAGAGGAC ACAATCCCAA AGAAGGTGGT GTGCCACTTC 
AGCGGGAAGG CCTACGCTGA CGAGGAGCGC TGGGACATCG ACAGCTGCAC GCACTGCTAC 
TGCCTGCAGG GCCAGACCCT CTGCTCCACC GTCAGCTGCC CGCCGCTGCC CTGCGTCGAG 
CCCATCAACG TGGAAGGCAG CTGCTGCCCC ATGTGCCCAG AAATGTATGT CCCAGAACCA 
ACCAATATAC CCATTGAGAA GACAAATCAT CGGGAGATCG ACATGGAGGT CCCCCTGTGG 
CCCACACCTA GTGAAAATGA CATTATCCAT CTCCCCAGAG ACATGGGTCA CCCCCAGATA 
GATTACAGAG ATAATACCAG GCTGAACTCA GAAGATTCTT CAGTGGACTC CATTGCCTCG 
GTTGTGGTTC CCATAATAAT AGGCCTCTCT ATCATAATAG CATTCCTGTT CATCAATCAG 
AAGAAACAGT GGATACCACT GCTTTGCTGG TATCGAACAC CAACTAAGCC TTCTTCTTTA 
AATAATCAGC TGGTATCTGT GGACTGCAAG AAAGGAACCC GGGTCCAGGT GGACAGTTCC 
CAGAGAATGC TGAGAATCGC GGAACCAGAC GCGAGATTCA GTGGCTTCTA CAGCATGCAG 
AAACAGAACC ACCTACAGGC AGACAATTTC TACCAAACAG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRIM1 ( XM_006056852.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006056914.1 Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu13902
Clone ID Related Accession (Same CDS sequence)	XM_006056852.1
Accession Version	XM_006056852.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	cysteine-rich motor neuron 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784779.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCT GGTCTCGCTG 
CTGGGGCTGT TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAAGA GCCCAAGAGC TGCCCCGGGA GCATCGTGCA GGGCTTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGA AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAACTGCTT 
GGGTTTGAAC CATGCAATGA AAACCTTGTC TCTGGCTGCA ATCTCATCAA TGGAAAATGT 
GAGTGTGACA CCATTCGAAC CTGTAACAAT CCCTTCGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TAAAGAGGAT TGAAGAAGAG AAGCCAGATT GCTCCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCACGTTG TCCTGAAGAC TCCGTTCTGA TCGAGGGCTA CGCGCCCCCC 
GGGGAGTGCT GCCCCCTCCC CAGCCGCTGT GTGTGTGACC CCGCGGGCTG CCTGCGGAAA 
GTCTGCCAGC CAGGATACCT GAACATCCTT GTGTCCAAAG CCTCAGGGAA GCCGGGAGAG 
TGCTGTGACC TCTATGAGTG CAAACCAGTT TTCAGTGTGG ACTGCAGCAC CGTGGAGTGC 
CCCTCCTTTC AGCAAACCGT GTGCCCCCTG GACAGCTACG AAACCCAAGT CAGACTCACA 
GCGGATGGCT GCTGCACTCT TCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCCCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGCGT GTTCAACAAC 
GTGGAGTACA GTGACGGAGA CATGTTTCGA ATGGACAACT GTCGATTCTG TCGATGCCAA 
GGGGGTGTTT CCATCTGCTT CACTGCCCAG TGTGGTGAGC TGAGCTGTGA GAGATACTAC 
ATGCCCGAAG GGGAGTGCTG CCCTGTGTGT GAAGATCCTG TGTATCCTTT GAACAACCCC 
GCTGGCTGCT ATGCCAATGG CCAGATCCAC GCCCACGGGG ACCGGTGGCG GGAAGACGAC 
TGCACGTTCT GCCAGTGCAT CAATGGGGAG CCCCACTGCG TGGCGACGGC CTGTGGACAG 
AGCTGCATGA ACCCCGTCAA AGTGCCTGGG GAGTGCTGCC CTGTATGCGA AGAACCAACC 
TACATCACAA TTGATCCACC TGCATGTGGG GAGTTATCAA ACTGTACCCT GACAGAGAAG 
GACTGCATTT ATGGTTTCAA ACTCGATCAC AATGGTTGTC GAACCTGTCA GTGCAAAAAC 
AGGGAGGAGC TCTGCACGGG CCTCAGGCGA GGCTGCGCCT TGAACTGCCC CTTCGGCTTC 
CTAACCGACG CCTACGGCTG TGACGCCTGT CAGTGCCGCC CGCGGCCCCG GAAGTGCAGA 
CCCACAGCCT GCGAAAAGCA TTGTCCGTTC GGATACCTGA AGAATAAGCA CGGCTGTGAC 
ATCTGTCGCT GTAAGAAATG CCCAGAGCTC CGGTGCAGTA AGATCTGCTC CCTGGGTTTC 
CAGAAGGACA GTCATGGCTG TCTCATCTGC AAGTGCAGAG AGGTCTCTGC CTCCGCCGGG 
CCACCCGTCC TGTCAGGCAC GTGTCTATCC ATGGATGGCC ATCATCATAA AAATGAGGAG 
AGCTGGCATG ATGGGTGTCG GGAATGCTAC TGCCACAGTG GACGGGAGAT GTGCGCACTG 
ATCACCTGCC CCGTGCCTGC CTGTGGCAAC CCCACCATTC ACCCGGGACG GTGCTGCCCA 
TCATGTTCAG ATGACTTCGT GGTGCAGAAG CCAGAGCTCA GCACGCCCTC CATCTGCCAC 
GCCCCCGGGG GAGAGTACTT TGTGGAGGGA GAAACGTGGC ACATTGACAC CTGCACGCAA 
TGCACGTGCC ACAGCGGGCG GGTGCTGTGT GAGACGGAGG TGTGCCCGCC GCTGCTCTGC 
CAGAACCCCA CACGCACCCA GGACTCCTGC TGCCCACAGT GCCCAGATGA ACCTCTCCAG 
CCTTCCTCAT CCCACAATGA CAGTGTGCCT AGTTACTGCA AAAACGATGA AGGGGATATA 
TTCCTGGCAG CTGAGTCCTG GAAACCCGAC GTGTGTACCA GCTGCGTGTG CATGGATAGC 
GTCATTAGCT GTTACTCTGA GTCTTGCCCT TCTGTATCCT GTGAAAGACC TGTGTTGAGA 
AAAGGCCAGT GTTGTCCCTA CTGCATAGAG GACACAATCC CAAAGAAGGT GGTGTGCCAC 
TTCAGCGGGA AGGCCTACGC TGACGAGGAG CGCTGGGACA TCGACAGCTG CACGCACTGC 
TACTGCCTGC AGGGCCAGAC CCTCTGCTCC ACCGTCAGCT GCCCGCCGCT GCCCTGCGTC 
GAGCCCATCA ACGTGGAAGG CAGCTGCTGC CCCATGTGCC CAGAAATGTA TGTCCCAGAA 
CCAACCAATA TACCCATTGA GAAGACAAAT CATCGGGAGA TCGACATGGA GGTCCCCCTG 
TGGCCCACAC CTAGTGAAAA TGACATTATC CATCTCCCCA GAGACATGGG TCACCCCCAG 
ATAGATTACA GAGATAATAC CAGGCTGAAC TCAGAAGATT CTTCAGTGGA CTCCATTGCC 
TCGGTTGTGG TTCCCATAAT AATAGGCCTC TCTATCATAA TAGCATTCCT GTTCATCAAT 
CAGAAGAAAC AGTGGATACC ACTGCTTTGC TGGTATCGAA CACCAACTAA GCCTTCTTCT 
TTAAATAATC AGCTGGTATC TGTGGACTGC AAGAAAGGAA CCCGGGTCCA GGTGGACAGT 
TCCCAGAGAA TGCTGAGAAT CGCGGAACCA GACGCGAGAT TCAGTGGCTT CTACAGCATG 
CAGAAACAGA ACCACCTACA GGCAGACAAT TTCTACCAAA CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006056914.1
CDS	60..3167
Translation	MYLVAGGRGLAGCGHLLVSLLGLLLLLARSGTRALVCLPCDESKCEEPKSCPGSIVQGFCGCCYMCARQRNESCGGAYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLLGFEPCNENLVSGCNLINGKCECDTIRTCNNPFEFPRKDMCLSALKRIEEEKPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCDPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPSFQQTVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPVCEVGSTPRIVSRGDGTPGKCCDVFECVNETKPACVFNNVEYSDGDMFRMDNCRFCRCQGGVSICFTAQCGELSCERYYMPEGECCPVCEDPVYPLNNPAGCYANGQIHAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLTEKDCIYGFKLDHNGCRTCQCKNREELCTGLRRGCALNCPFGFLTDAYGCDACQCRPRPRKCRPTACEKHCPFGYLKNKHGCDICRCKKCPELRCSKICSLGFQKDSHGCLICKCREVSASAGPPVLSGTCLSMDGHHHKNEESWHDGCRECYCHSGREMCALITCPVPACGNPTIHPGRCCPSCSDDFVVQKPELSTPSICHAPGGEYFVEGETWHIDTCTQCTCHSGRVLCETEVCPPLLCQNPTRTQDSCCPQCPDEPLQPSSSHNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCYSESCPSVSCERPVLRKGQCCPYCIEDTIPKKVVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHREIDMEVPLWPTPSENDIIHLPRDMGHPQIDYRDNTRLNSEDSSVDSIASVVVPIIIGLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGTRVQVDSSQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_006056852.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006056852.1

ORF Insert Sequence:

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCT GGTCTCGCTG 
CTGGGGCTGT TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAAGA GCCCAAGAGC TGCCCCGGGA GCATCGTGCA GGGCTTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGA AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAACTGCTT 
GGGTTTGAAC CATGCAATGA AAACCTTGTC TCTGGCTGCA ATCTCATCAA TGGAAAATGT 
GAGTGTGACA CCATTCGAAC CTGTAACAAT CCCTTCGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TAAAGAGGAT TGAAGAAGAG AAGCCAGATT GCTCCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCACGTTG TCCTGAAGAC TCCGTTCTGA TCGAGGGCTA CGCGCCCCCC 
GGGGAGTGCT GCCCCCTCCC CAGCCGCTGT GTGTGTGACC CCGCGGGCTG CCTGCGGAAA 
GTCTGCCAGC CAGGATACCT GAACATCCTT GTGTCCAAAG CCTCAGGGAA GCCGGGAGAG 
TGCTGTGACC TCTATGAGTG CAAACCAGTT TTCAGTGTGG ACTGCAGCAC CGTGGAGTGC 
CCCTCCTTTC AGCAAACCGT GTGCCCCCTG GACAGCTACG AAACCCAAGT CAGACTCACA 
GCGGATGGCT GCTGCACTCT TCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCCCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGCGT GTTCAACAAC 
GTGGAGTACA GTGACGGAGA CATGTTTCGA ATGGACAACT GTCGATTCTG TCGATGCCAA 
GGGGGTGTTT CCATCTGCTT CACTGCCCAG TGTGGTGAGC TGAGCTGTGA GAGATACTAC 
ATGCCCGAAG GGGAGTGCTG CCCTGTGTGT GAAGATCCTG TGTATCCTTT GAACAACCCC 
GCTGGCTGCT ATGCCAATGG CCAGATCCAC GCCCACGGGG ACCGGTGGCG GGAAGACGAC 
TGCACGTTCT GCCAGTGCAT CAATGGGGAG CCCCACTGCG TGGCGACGGC CTGTGGACAG 
AGCTGCATGA ACCCCGTCAA AGTGCCTGGG GAGTGCTGCC CTGTATGCGA AGAACCAACC 
TACATCACAA TTGATCCACC TGCATGTGGG GAGTTATCAA ACTGTACCCT GACAGAGAAG 
GACTGCATTT ATGGTTTCAA ACTCGATCAC AATGGTTGTC GAACCTGTCA GTGCAAAAAC 
AGGGAGGAGC TCTGCACGGG CCTCAGGCGA GGCTGCGCCT TGAACTGCCC CTTCGGCTTC 
CTAACCGACG CCTACGGCTG TGACGCCTGT CAGTGCCGCC CGCGGCCCCG GAAGTGCAGA 
CCCACAGCCT GCGAAAAGCA TTGTCCGTTC GGATACCTGA AGAATAAGCA CGGCTGTGAC 
ATCTGTCGCT GTAAGAAATG CCCAGAGCTC CGGTGCAGTA AGATCTGCTC CCTGGGTTTC 
CAGAAGGACA GTCATGGCTG TCTCATCTGC AAGTGCAGAG AGGTCTCTGC CTCCGCCGGG 
CCACCCGTCC TGTCAGGCAC GTGTCTATCC ATGGATGGCC ATCATCATAA AAATGAGGAG 
AGCTGGCATG ATGGGTGTCG GGAATGCTAC TGCCACAGTG GACGGGAGAT GTGCGCACTG 
ATCACCTGCC CCGTGCCTGC CTGTGGCAAC CCCACCATTC ACCCGGGACG GTGCTGCCCA 
TCATGTTCAG ATGACTTCGT GGTGCAGAAG CCAGAGCTCA GCACGCCCTC CATCTGCCAC 
GCCCCCGGGG GAGAGTACTT TGTGGAGGGA GAAACGTGGC ACATTGACAC CTGCACGCAA 
TGCACGTGCC ACAGCGGGCG GGTGCTGTGT GAGACGGAGG TGTGCCCGCC GCTGCTCTGC 
CAGAACCCCA CACGCACCCA GGACTCCTGC TGCCCACAGT GCCCAGATGA ACCTCTCCAG 
CCTTCCTCAT CCCACAATGA CAGTGTGCCT AGTTACTGCA AAAACGATGA AGGGGATATA 
TTCCTGGCAG CTGAGTCCTG GAAACCCGAC GTGTGTACCA GCTGCGTGTG CATGGATAGC 
GTCATTAGCT GTTACTCTGA GTCTTGCCCT TCTGTATCCT GTGAAAGACC TGTGTTGAGA 
AAAGGCCAGT GTTGTCCCTA CTGCATAGAG GACACAATCC CAAAGAAGGT GGTGTGCCAC 
TTCAGCGGGA AGGCCTACGC TGACGAGGAG CGCTGGGACA TCGACAGCTG CACGCACTGC 
TACTGCCTGC AGGGCCAGAC CCTCTGCTCC ACCGTCAGCT GCCCGCCGCT GCCCTGCGTC 
GAGCCCATCA ACGTGGAAGG CAGCTGCTGC CCCATGTGCC CAGAAATGTA TGTCCCAGAA 
CCAACCAATA TACCCATTGA GAAGACAAAT CATCGGGAGA TCGACATGGA GGTCCCCCTG 
TGGCCCACAC CTAGTGAAAA TGACATTATC CATCTCCCCA GAGACATGGG TCACCCCCAG 
ATAGATTACA GAGATAATAC CAGGCTGAAC TCAGAAGATT CTTCAGTGGA CTCCATTGCC 
TCGGTTGTGG TTCCCATAAT AATAGGCCTC TCTATCATAA TAGCATTCCT GTTCATCAAT 
CAGAAGAAAC AGTGGATACC ACTGCTTTGC TGGTATCGAA CACCAACTAA GCCTTCTTCT 
TTAAATAATC AGCTGGTATC TGTGGACTGC AAGAAAGGAA CCCGGGTCCA GGTGGACAGT 
TCCCAGAGAA TGCTGAGAAT CGCGGAACCA GACGCGAGAT TCAGTGGCTT CTACAGCATG 
CAGAAACAGA ACCACCTACA GGCAGACAAT TTCTACCAAA CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRIM1 ( XM_006056852.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006056914.1 Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (CRIM1), transcript variant X1, mRNA.

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CloneID	OBu45931
Clone ID Related Accession (Same CDS sequence)	XM_006056852.2
Accession Version	XM_006056852.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cysteine-rich motor neuron 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006056852.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCT GGTCTCGCTG 
CTGGGGCTGT TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAAGA GCCCAAGAGC TGCCCCGGGA GCATCGTGCA GGGCTTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGA AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAACTGCTT 
GGGTTTGAAC CATGCAATGA AAACCTTGTC TCTGGCTGCA ATCTCATCAA TGGAAAATGT 
GAGTGTGACA CCATTCGAAC CTGTAACAAT CCCTTCGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TAAAGAGGAT TGAAGAAGAG AAGCCAGATT GCTCCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCACGTTG TCCTGAAGAC TCCGTTCTGA TCGAGGGCTA CGCGCCCCCC 
GGGGAGTGCT GCCCCCTCCC CAGCCGCTGT GTGTGTGACC CCGCGGGCTG CCTGCGGAAA 
GTCTGCCAGC CAGGATACCT GAACATCCTT GTGTCCAAAG CCTCAGGGAA GCCGGGAGAG 
TGCTGTGACC TCTATGAGTG CAAACCAGTT TTCAGTGTGG ACTGCAGCAC CGTGGAGTGC 
CCCTCCTTTC AGCAAACCGT GTGCCCCCTG GACAGCTACG AAACCCAAGT CAGACTCACA 
GCGGATGGCT GCTGCACTCT TCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCCCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGCGT GTTCAACAAC 
GTGGAGTACA GTGACGGAGA CATGTTTCGA ATGGACAACT GTCGATTCTG TCGATGCCAA 
GGGGGTGTTT CCATCTGCTT CACTGCCCAG TGTGGTGAGC TGAGCTGTGA GAGATACTAC 
ATGCCCGAAG GGGAGTGCTG CCCTGTGTGT GAAGATCCTG TGTATCCTTT GAACAACCCC 
GCTGGCTGCT ATGCCAATGG CCAGATCCAC GCCCACGGGG ACCGGTGGCG GGAAGACGAC 
TGCACGTTCT GCCAGTGCAT CAATGGGGAG CCCCACTGCG TGGCGACGGC CTGTGGACAG 
AGCTGCATGA ACCCCGTCAA AGTGCCTGGG GAGTGCTGCC CTGTATGCGA AGAACCAACC 
TACATCACAA TTGATCCACC TGCATGTGGG GAGTTATCAA ACTGTACCCT GACAGAGAAG 
GACTGCATTT ATGGTTTCAA ACTCGATCAC AATGGTTGTC GAACCTGTCA GTGCAAAAAC 
AGGGAGGAGC TCTGCACGGG CCTCAGGCGA GGCTGCGCCT TGAACTGCCC CTTCGGCTTC 
CTAACCGACG CCTACGGCTG TGACGCCTGT CAGTGCCGCC CGCGCCCCCG GAAGTGCAGA 
CCCACAGCCT GCGAAAAGCA TTGTCCGTTC GGATACCTGA AGAATAAGCA CGGCTGTGAC 
ATCTGTCGCT GTAAGAAATG CCCAGAGCTC CGGTGCAGTA AGATCTGCTC CCTGGGTTTC 
CAGAAGGACA GTCATGGCTG TCTCATCTGC AAGTGCAGAG AGGTCTCTGC CTCCGCCGGG 
CCACCCGTCC TGTCAGGCAC GTGTCTATCC ATGGATGGCC ATCATCATAA AAATGAGGAG 
AGCTGGCATG ATGGGTGTCG GGAATGCTAC TGCCACAGTG GACGGGAGAT GTGCGCACTG 
ATCACCTGCC CCGTGCCTGC CTGTGGCAAC CCCACCATTC ACCCGGGACG GTGCTGCCCA 
TCATGTTCAG ATGACTTCGT GGTGCAGAAG CCAGAGCTCA GCACGCCCTC CATCTGCCAC 
GCCCCCGGGG GAGAGTACTT TGTGGAGGGA GAAACATGGC ACATTGACAC CTGCACGCAA 
TGCACGTGCC ACAGCGGGCG GGTGCTGTGT GAGACGGAGG TGTGCCCGCC GCTGCTCTGC 
CAGAACCCCA CACGCACCCA GGACTCCTGC TGCCCACAGT GCCCAGATGA ACCTCTCCAG 
CCTTCCTCAT CCCACAATGA CAGTGTGCCT AGTTACTGCA AAAACGATGA AGGGGATATA 
TTCCTGGCAG CTGAGTCCTG GAAACCCGAC GTGTGTACCA GCTGCGTGTG CATGGATAGC 
GTCATTAGCT GTTACTCTGA GTCTTGCCCT TCTGTATCCT GTGAAAGACC TGTGTTGAGA 
AAAGGCCAGT GTTGTCCCTA CTGCATAGAG GACACAATCC CAAAGAAGGT GGTGTGCCAC 
TTCAGCGGGA AGGCCTACGC TGACGAGGAG CGCTGGGACA TCGACAGCTG CACGCACTGC 
TACTGCCTGC AGGGCCAGAC CCTCTGCTCC ACCGTCAGCT GCCCGCCGCT GCCCTGCGTC 
GAGCCCATCA ACGTGGAAGG CAGCTGCTGC CCCATGTGCC CAGAAATGTA TGTCCCAGAA 
CCAACCAATA TACCCATTGA GAAGACAAAT CATCGGGAGA TCGACATGGA GGTCCCCCTG 
TGGCCCACAC CTAGTGAAAA TGACATTATC CATCTCCCCA GAGACATGGG TCACCCCCAG 
ATAGATTACA GAGATAATAC CAGGCTGAAC TCAGAAGATT CTTCAGTGGA CTCCATTGCC 
TCGGTTGTGG TTCCCATAAT AATAGGCCTC TCTATCATAA TAGCATTCCT GTTCATCAAT 
CAGAAGAAAC AGTGGATACC ACTGCTTTGC TGGTATCGAA CACCAACTAA GCCTTCTTCT 
TTAAATAATC AGCTGGTATC TGTGGACTGC AAGAAAGGAA CCCGGGTCCA GGTGGACAGT 
TCCCAGAGAA TGCTGAGAAT CGCGGAACCA GACGCGAGAT TCAGTGGCTT CTACAGCATG 
CAGAAACAGA ACCACCTACA GGCAGACAAT TTCTACCAAA CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006056914.1
CDS	533..3640
Translation	MYLVAGGRGLAGCGHLLVSLLGLLLLLARSGTRALVCLPCDESKCEEPKSCPGSIVQGFCGCCYMCARQRNESCGGAYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLLGFEPCNENLVSGCNLINGKCECDTIRTCNNPFEFPRKDMCLSALKRIEEEKPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCDPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPSFQQTVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPVCEVGSTPRIVSRGDGTPGKCCDVFECVNETKPACVFNNVEYSDGDMFRMDNCRFCRCQGGVSICFTAQCGELSCERYYMPEGECCPVCEDPVYPLNNPAGCYANGQIHAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLTEKDCIYGFKLDHNGCRTCQCKNREELCTGLRRGCALNCPFGFLTDAYGCDACQCRPRPRKCRPTACEKHCPFGYLKNKHGCDICRCKKCPELRCSKICSLGFQKDSHGCLICKCREVSASAGPPVLSGTCLSMDGHHHKNEESWHDGCRECYCHSGREMCALITCPVPACGNPTIHPGRCCPSCSDDFVVQKPELSTPSICHAPGGEYFVEGETWHIDTCTQCTCHSGRVLCETEVCPPLLCQNPTRTQDSCCPQCPDEPLQPSSSHNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCYSESCPSVSCERPVLRKGQCCPYCIEDTIPKKVVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHREIDMEVPLWPTPSENDIIHLPRDMGHPQIDYRDNTRLNSEDSSVDSIASVVVPIIIGLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGTRVQVDSSQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_006056852.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cysteine rich transmembrane BMP regulator 1 (CRIM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006056852.2

ORF Insert Sequence:

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCT GGTCTCGCTG 
CTGGGGCTGT TGCTGCTGCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAAGA GCCCAAGAGC TGCCCCGGGA GCATCGTGCA GGGCTTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGA AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACCGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAACTGCTT 
GGGTTTGAAC CATGCAATGA AAACCTTGTC TCTGGCTGCA ATCTCATCAA TGGAAAATGT 
GAGTGTGACA CCATTCGAAC CTGTAACAAT CCCTTCGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TAAAGAGGAT TGAAGAAGAG AAGCCAGATT GCTCCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCACGTTG TCCTGAAGAC TCCGTTCTGA TCGAGGGCTA CGCGCCCCCC 
GGGGAGTGCT GCCCCCTCCC CAGCCGCTGT GTGTGTGACC CCGCGGGCTG CCTGCGGAAA 
GTCTGCCAGC CAGGATACCT GAACATCCTT GTGTCCAAAG CCTCAGGGAA GCCGGGAGAG 
TGCTGTGACC TCTATGAGTG CAAACCAGTT TTCAGTGTGG ACTGCAGCAC CGTGGAGTGC 
CCCTCCTTTC AGCAAACCGT GTGCCCCCTG GACAGCTACG AAACCCAAGT CAGACTCACA 
GCGGATGGCT GCTGCACTCT TCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCCCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGCGT GTTCAACAAC 
GTGGAGTACA GTGACGGAGA CATGTTTCGA ATGGACAACT GTCGATTCTG TCGATGCCAA 
GGGGGTGTTT CCATCTGCTT CACTGCCCAG TGTGGTGAGC TGAGCTGTGA GAGATACTAC 
ATGCCCGAAG GGGAGTGCTG CCCTGTGTGT GAAGATCCTG TGTATCCTTT GAACAACCCC 
GCTGGCTGCT ATGCCAATGG CCAGATCCAC GCCCACGGGG ACCGGTGGCG GGAAGACGAC 
TGCACGTTCT GCCAGTGCAT CAATGGGGAG CCCCACTGCG TGGCGACGGC CTGTGGACAG 
AGCTGCATGA ACCCCGTCAA AGTGCCTGGG GAGTGCTGCC CTGTATGCGA AGAACCAACC 
TACATCACAA TTGATCCACC TGCATGTGGG GAGTTATCAA ACTGTACCCT GACAGAGAAG 
GACTGCATTT ATGGTTTCAA ACTCGATCAC AATGGTTGTC GAACCTGTCA GTGCAAAAAC 
AGGGAGGAGC TCTGCACGGG CCTCAGGCGA GGCTGCGCCT TGAACTGCCC CTTCGGCTTC 
CTAACCGACG CCTACGGCTG TGACGCCTGT CAGTGCCGCC CGCGCCCCCG GAAGTGCAGA 
CCCACAGCCT GCGAAAAGCA TTGTCCGTTC GGATACCTGA AGAATAAGCA CGGCTGTGAC 
ATCTGTCGCT GTAAGAAATG CCCAGAGCTC CGGTGCAGTA AGATCTGCTC CCTGGGTTTC 
CAGAAGGACA GTCATGGCTG TCTCATCTGC AAGTGCAGAG AGGTCTCTGC CTCCGCCGGG 
CCACCCGTCC TGTCAGGCAC GTGTCTATCC ATGGATGGCC ATCATCATAA AAATGAGGAG 
AGCTGGCATG ATGGGTGTCG GGAATGCTAC TGCCACAGTG GACGGGAGAT GTGCGCACTG 
ATCACCTGCC CCGTGCCTGC CTGTGGCAAC CCCACCATTC ACCCGGGACG GTGCTGCCCA 
TCATGTTCAG ATGACTTCGT GGTGCAGAAG CCAGAGCTCA GCACGCCCTC CATCTGCCAC 
GCCCCCGGGG GAGAGTACTT TGTGGAGGGA GAAACATGGC ACATTGACAC CTGCACGCAA 
TGCACGTGCC ACAGCGGGCG GGTGCTGTGT GAGACGGAGG TGTGCCCGCC GCTGCTCTGC 
CAGAACCCCA CACGCACCCA GGACTCCTGC TGCCCACAGT GCCCAGATGA ACCTCTCCAG 
CCTTCCTCAT CCCACAATGA CAGTGTGCCT AGTTACTGCA AAAACGATGA AGGGGATATA 
TTCCTGGCAG CTGAGTCCTG GAAACCCGAC GTGTGTACCA GCTGCGTGTG CATGGATAGC 
GTCATTAGCT GTTACTCTGA GTCTTGCCCT TCTGTATCCT GTGAAAGACC TGTGTTGAGA 
AAAGGCCAGT GTTGTCCCTA CTGCATAGAG GACACAATCC CAAAGAAGGT GGTGTGCCAC 
TTCAGCGGGA AGGCCTACGC TGACGAGGAG CGCTGGGACA TCGACAGCTG CACGCACTGC 
TACTGCCTGC AGGGCCAGAC CCTCTGCTCC ACCGTCAGCT GCCCGCCGCT GCCCTGCGTC 
GAGCCCATCA ACGTGGAAGG CAGCTGCTGC CCCATGTGCC CAGAAATGTA TGTCCCAGAA 
CCAACCAATA TACCCATTGA GAAGACAAAT CATCGGGAGA TCGACATGGA GGTCCCCCTG 
TGGCCCACAC CTAGTGAAAA TGACATTATC CATCTCCCCA GAGACATGGG TCACCCCCAG 
ATAGATTACA GAGATAATAC CAGGCTGAAC TCAGAAGATT CTTCAGTGGA CTCCATTGCC 
TCGGTTGTGG TTCCCATAAT AATAGGCCTC TCTATCATAA TAGCATTCCT GTTCATCAAT 
CAGAAGAAAC AGTGGATACC ACTGCTTTGC TGGTATCGAA CACCAACTAA GCCTTCTTCT 
TTAAATAATC AGCTGGTATC TGTGGACTGC AAGAAAGGAA CCCGGGTCCA GGTGGACAGT 
TCCCAGAGAA TGCTGAGAAT CGCGGAACCA GACGCGAGAT TCAGTGGCTT CTACAGCATG 
CAGAAACAGA ACCACCTACA GGCAGACAAT TTCTACCAAA CAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CRIM1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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