AP2B1 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following AP2B1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AP2B1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu13375 XM_006056222.2
Latest version!
Bubalus bubalis adaptor related protein complex 2 subunit beta 1 (AP2B1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu13375 XM_025281091.1
Latest version!
Bubalus bubalis adaptor related protein complex 2 subunit beta 1 (AP2B1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu13375 XM_025281092.1
Latest version!
Bubalus bubalis adaptor related protein complex 2 subunit beta 1 (AP2B1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu13375 XM_006056221.2
Latest version!
Bubalus bubalis adaptor related protein complex 2 subunit beta 1 (AP2B1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu13376 XM_006056223.2
Latest version!
Bubalus bubalis adaptor related protein complex 2 subunit beta 1 (AP2B1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu62377 XM_025281093.1
Latest version!
Bubalus bubalis adaptor related protein complex 2 subunit beta 1 (AP2B1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
View Old Accession Versions >>
OBu13375 XM_006056221.1 Bubalus bubalis adaptor-related protein complex 2, beta 1 subunit (AP2B1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu13376 XM_006056223.1 Bubalus bubalis adaptor-related protein complex 2, beta 1 subunit (AP2B1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu13375 XM_006056222.1 Bubalus bubalis adaptor-related protein complex 2, beta 1 subunit (AP2B1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu13375
    Clone ID Related Accession (Same CDS sequence) XM_006056222.1 , XM_006056221.1 , XM_006056221.2 , XM_025281091.1 , XM_025281092.1 , XM_006056222.2
    Accession Version XM_006056222.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product AP-1 complex subunit beta-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784736.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGACTGACT CCAAGTACTT CACAACCAAT AAAAAGGGAG AAATCTTTGA ATTAAAGGCT 
    GAACTCAACA ATGAAAAGAA AGAAAAGAGG AAGGAGGCTG TGAAGAAAGT GATTGCTGCT
    ATGACTGTGG GGAAAGACGT TAGCTCTCTT TTTCCAGATG TAGTGAACTG TATGCAGACG
    GATAATCTGG AACTGAAGAA GCTTGTGTAT CTCTACTTGA TGAACTATGC CAAGAGTCAG
    CCAGACATGG CCATCATGGC TGTCAACAGC TTTGTGAAGG ATTGTGAAGA TCCCAATCCT
    CTGATTCGAG CCTTGGCAGT CAGAACCATG GGGTGCATCC GGGTGGACAA GATTACAGAG
    TATCTCTGTG AGCCCCTCCG CAAGTGCTTA AAGGATGAAG ATCCCTACGT CCGGAAGACA
    GCAGCAGTCT GTGTGGCAAA ACTCCATGAC ATCAATGCCC AGATGGTGGA AGATCAGGGA
    TTTCTGGATT CTCTGCGGGA TCTCATAGCA GATTCAAATC CAATGGTGGT GGCTAATGCT
    GTAGCAGCAC TATCTGAAAT CAGTGAGTCT CACCCCAACA GCAACTTACT CGATCTGAAT
    CCACAGAACA TTAATAAGCT ACTGACAGCC CTGAATGAGT GCACCGAATG GGGCCAGATT
    TTCATCCTGG ACTGCTTATC TAATTACAAT CCTAAAGATG ACCGGGAGGC TCAGAGCATC
    TGTGAGCGGG TAACTCCCCG GTTATCTCAT GCCAACTCAG CAGTGGTGCT TTCAGCAGTA
    AAAGTCCTAA TGAAATTTTT AGAGTTGCTG CCCAAGGACT CTGACTACTA CAGTATGCTG
    CTGAAGAAGT TGGCCCCTCC ACTTGTCACT CTGCTGTCTG GGGAGCCAGA GGTGCAATAC
    GTCGCCCTGA GGAACATCAA CCTAATTGTT CAGAAAAGGC CTGAAATCTT GAAGCAGGAA
    ATAAAAGTTT TCTTTGTGAA GTACAATGAT CCCATCTATG TGAAACTAGA GAAGTTGGAC
    ATCATGATTC GTTTGGCATC CCAAGCCAAC ATTGCTCAGG TTCTGGCTGA ACTGAAAGAG
    TATGCCACAG AGGTGGATGT TGACTTTGTG CGCAAAGCTG TGCGAGCCAT TGGGCGGTGT
    GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CGTTGCTTGA TCTCATCCAA
    ACCAAAGTAA ATTATGTGGT CCAAGAGGCG ATTGTTGTCA TCAGGGACAT TTTCCGCAAA
    TACCCCAACA AATACGAAAG TATCATTGCC ACACTGTGTG AGAACTTAGA TTCTCTGGAT
    GAGCCAGACG CTCGAGCAGC TATGATTTGG ATTGTGGGAG AGTATGCTGA AAGAATTGAC
    AATGCTGATG AGTTACTAGA GAGCTTCCTG GAGGGTTTTC ATGATGAAAG CACCCAAGTG
    CAGCTCACTC TGCTCACTGC CATAGTGAAG CTGTTTCTCA AGAAACCGTC AGAAACACAG
    GAGCTGGTAC AGCAGGTCTT GAGTTTGGCA ACACAGGATT CTGATAATCC TGACCTTCGA
    GACCGGGGCT ATATTTATTG GCGCCTTCTC TCAACTGATC CTGTCACAGC TAAAGAAGTA
    GTCTTGTCTG AAAAGCCACT GATTTCTGAG GAGACAGATC TTATTGAACC AACTCTACTG
    GATGAGCTAA TCTGCCACAT TGGTTCTTTG GCCTCAGTGT ACCATAAGCC GCCCAATGCT
    TTTGTGGAAG GAAGTCATGG AATCCATCGC AAACACTTGC CAATACATCA TGGGAGCACT
    GATGCAGGTG ACAGCCCTGT TGGCGCCGCC ACTGCCACCA ACCTGGAGCA GCCTCAGGTT
    ATTCCCTCCC AGGGTGACCT TCTGGGGGAT CTTTTAAACC TTGACCTTGG TCCCCCAGTC
    AATGTGCCAC AGGTATCTTC CATGCAGATG GGAGCAGTGG ATCTCTTGGG AGGAGGCCTA
    GATAGTCTGC TTGGCAGTGA CCTTGGCGGG GGCATTGGAG GAAGTCCGGC AGTGGGACAG
    TCCTTCATCC CCTCATCAGT GCCTGCAACC TTTGCTCCCT CCCCTACTCC TGCTGTGGTC
    AGCAGTGGTC TGAATGACCT GTTTGAACTC TCCACGGGGA TAGGCATGGC ACCTGGTGGA
    TATGTGGCTC CTAAGGCTGT CTGGCTGCCT GCGGTAAAGG CTAAAGGCCT GGAGATTTCA
    GGAACATTTA CTCACCGCCA AGGGCACATC TATATGGAAA TGAACTTCAC CAACAAAGCT
    CTGCAGCACA TGACAGACTT CGCAATTCAG TTTAACAAGA ATAGCTTTGG TGTCATCCCT
    AGCACTCCTC TGGCCATCCA TACGCCACTG ATGCCAAACC AGAGCATTGA TGTCTCCCTG
    CCCCTCAACA CCTTGGGCCC TGTCATGAAG ATGGAGCCTC TGAACAACCT GCAGGTGGCT
    GTGAAAAACA ATATTGATGT CTTCTACTTC AGCTGCCTCA TCCCACTCAA CGTGCTTTTT
    GTAGAAGATG GCAAAATGGA GCGCCAGGTC TTCCTTGCAA CATGGAAGGA TATTCCCAAT
    GAAAATGAAC TTCAGTTTCA GATTAAGGAA TGTCATTTAA ATGCTGACAC TGTTTCCAGC
    AAGTTGCAAA ACAACAATGT TTATACTATT GCCAAGAGGA ATGTGGAAGG GCAGGACATG
    CTGTACCAAT CCCTGAAGCT CACTAATGGC ATTTGGATTT TGGCCGAGCT ACGTATCCAA
    CCAGGAAACC CCAATTACAC GCTGTCGCTG AAGTGTAGAG CTCCCGAGGT CTCCCAGTAC
    ATCTATCAGG TCTACGACAG CATTTTGAAA AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006056284.1
    CDS386..3241
    Translation

    Target ORF information:

    RefSeq Version XM_006056222.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis adaptor-related protein complex 2, beta 1 subunit (AP2B1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006056222.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGACTGACT CCAAGTACTT CACAACCAAT AAAAAGGGAG AAATCTTTGA ATTAAAGGCT 
    GAACTCAACA ATGAAAAGAA AGAAAAGAGG AAGGAGGCTG TGAAGAAAGT GATTGCTGCT
    ATGACTGTGG GGAAAGACGT TAGCTCTCTT TTTCCAGATG TAGTGAACTG TATGCAGACG
    GATAATCTGG AACTGAAGAA GCTTGTGTAT CTCTACTTGA TGAACTATGC CAAGAGTCAG
    CCAGACATGG CCATCATGGC TGTCAACAGC TTTGTGAAGG ATTGTGAAGA TCCCAATCCT
    CTGATTCGAG CCTTGGCAGT CAGAACCATG GGGTGCATCC GGGTGGACAA GATTACAGAG
    TATCTCTGTG AGCCCCTCCG CAAGTGCTTA AAGGATGAAG ATCCCTACGT CCGGAAGACA
    GCAGCAGTCT GTGTGGCAAA ACTCCATGAC ATCAATGCCC AGATGGTGGA AGATCAGGGA
    TTTCTGGATT CTCTGCGGGA TCTCATAGCA GATTCAAATC CAATGGTGGT GGCTAATGCT
    GTAGCAGCAC TATCTGAAAT CAGTGAGTCT CACCCCAACA GCAACTTACT CGATCTGAAT
    CCACAGAACA TTAATAAGCT ACTGACAGCC CTGAATGAGT GCACCGAATG GGGCCAGATT
    TTCATCCTGG ACTGCTTATC TAATTACAAT CCTAAAGATG ACCGGGAGGC TCAGAGCATC
    TGTGAGCGGG TAACTCCCCG GTTATCTCAT GCCAACTCAG CAGTGGTGCT TTCAGCAGTA
    AAAGTCCTAA TGAAATTTTT AGAGTTGCTG CCCAAGGACT CTGACTACTA CAGTATGCTG
    CTGAAGAAGT TGGCCCCTCC ACTTGTCACT CTGCTGTCTG GGGAGCCAGA GGTGCAATAC
    GTCGCCCTGA GGAACATCAA CCTAATTGTT CAGAAAAGGC CTGAAATCTT GAAGCAGGAA
    ATAAAAGTTT TCTTTGTGAA GTACAATGAT CCCATCTATG TGAAACTAGA GAAGTTGGAC
    ATCATGATTC GTTTGGCATC CCAAGCCAAC ATTGCTCAGG TTCTGGCTGA ACTGAAAGAG
    TATGCCACAG AGGTGGATGT TGACTTTGTG CGCAAAGCTG TGCGAGCCAT TGGGCGGTGT
    GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CGTTGCTTGA TCTCATCCAA
    ACCAAAGTAA ATTATGTGGT CCAAGAGGCG ATTGTTGTCA TCAGGGACAT TTTCCGCAAA
    TACCCCAACA AATACGAAAG TATCATTGCC ACACTGTGTG AGAACTTAGA TTCTCTGGAT
    GAGCCAGACG CTCGAGCAGC TATGATTTGG ATTGTGGGAG AGTATGCTGA AAGAATTGAC
    AATGCTGATG AGTTACTAGA GAGCTTCCTG GAGGGTTTTC ATGATGAAAG CACCCAAGTG
    CAGCTCACTC TGCTCACTGC CATAGTGAAG CTGTTTCTCA AGAAACCGTC AGAAACACAG
    GAGCTGGTAC AGCAGGTCTT GAGTTTGGCA ACACAGGATT CTGATAATCC TGACCTTCGA
    GACCGGGGCT ATATTTATTG GCGCCTTCTC TCAACTGATC CTGTCACAGC TAAAGAAGTA
    GTCTTGTCTG AAAAGCCACT GATTTCTGAG GAGACAGATC TTATTGAACC AACTCTACTG
    GATGAGCTAA TCTGCCACAT TGGTTCTTTG GCCTCAGTGT ACCATAAGCC GCCCAATGCT
    TTTGTGGAAG GAAGTCATGG AATCCATCGC AAACACTTGC CAATACATCA TGGGAGCACT
    GATGCAGGTG ACAGCCCTGT TGGCGCCGCC ACTGCCACCA ACCTGGAGCA GCCTCAGGTT
    ATTCCCTCCC AGGGTGACCT TCTGGGGGAT CTTTTAAACC TTGACCTTGG TCCCCCAGTC
    AATGTGCCAC AGGTATCTTC CATGCAGATG GGAGCAGTGG ATCTCTTGGG AGGAGGCCTA
    GATAGTCTGC TTGGCAGTGA CCTTGGCGGG GGCATTGGAG GAAGTCCGGC AGTGGGACAG
    TCCTTCATCC CCTCATCAGT GCCTGCAACC TTTGCTCCCT CCCCTACTCC TGCTGTGGTC
    AGCAGTGGTC TGAATGACCT GTTTGAACTC TCCACGGGGA TAGGCATGGC ACCTGGTGGA
    TATGTGGCTC CTAAGGCTGT CTGGCTGCCT GCGGTAAAGG CTAAAGGCCT GGAGATTTCA
    GGAACATTTA CTCACCGCCA AGGGCACATC TATATGGAAA TGAACTTCAC CAACAAAGCT
    CTGCAGCACA TGACAGACTT CGCAATTCAG TTTAACAAGA ATAGCTTTGG TGTCATCCCT
    AGCACTCCTC TGGCCATCCA TACGCCACTG ATGCCAAACC AGAGCATTGA TGTCTCCCTG
    CCCCTCAACA CCTTGGGCCC TGTCATGAAG ATGGAGCCTC TGAACAACCT GCAGGTGGCT
    GTGAAAAACA ATATTGATGT CTTCTACTTC AGCTGCCTCA TCCCACTCAA CGTGCTTTTT
    GTAGAAGATG GCAAAATGGA GCGCCAGGTC TTCCTTGCAA CATGGAAGGA TATTCCCAAT
    GAAAATGAAC TTCAGTTTCA GATTAAGGAA TGTCATTTAA ATGCTGACAC TGTTTCCAGC
    AAGTTGCAAA ACAACAATGT TTATACTATT GCCAAGAGGA ATGTGGAAGG GCAGGACATG
    CTGTACCAAT CCCTGAAGCT CACTAATGGC ATTTGGATTT TGGCCGAGCT ACGTATCCAA
    CCAGGAAACC CCAATTACAC GCTGTCGCTG AAGTGTAGAG CTCCCGAGGT CTCCCAGTAC
    ATCTATCAGG TCTACGACAG CATTTTGAAA AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu13375
    Clone ID Related Accession (Same CDS sequence) XM_006056222.1 , XM_006056221.1 , XM_006056221.2 , XM_025281091.1 , XM_025281092.1 , XM_006056222.2
    Accession Version XM_006056221.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product AP-1 complex subunit beta-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784736.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGACTGACT CCAAGTACTT CACAACCAAT AAAAAGGGAG AAATCTTTGA ATTAAAGGCT 
    GAACTCAACA ATGAAAAGAA AGAAAAGAGG AAGGAGGCTG TGAAGAAAGT GATTGCTGCT
    ATGACTGTGG GGAAAGACGT TAGCTCTCTT TTTCCAGATG TAGTGAACTG TATGCAGACG
    GATAATCTGG AACTGAAGAA GCTTGTGTAT CTCTACTTGA TGAACTATGC CAAGAGTCAG
    CCAGACATGG CCATCATGGC TGTCAACAGC TTTGTGAAGG ATTGTGAAGA TCCCAATCCT
    CTGATTCGAG CCTTGGCAGT CAGAACCATG GGGTGCATCC GGGTGGACAA GATTACAGAG
    TATCTCTGTG AGCCCCTCCG CAAGTGCTTA AAGGATGAAG ATCCCTACGT CCGGAAGACA
    GCAGCAGTCT GTGTGGCAAA ACTCCATGAC ATCAATGCCC AGATGGTGGA AGATCAGGGA
    TTTCTGGATT CTCTGCGGGA TCTCATAGCA GATTCAAATC CAATGGTGGT GGCTAATGCT
    GTAGCAGCAC TATCTGAAAT CAGTGAGTCT CACCCCAACA GCAACTTACT CGATCTGAAT
    CCACAGAACA TTAATAAGCT ACTGACAGCC CTGAATGAGT GCACCGAATG GGGCCAGATT
    TTCATCCTGG ACTGCTTATC TAATTACAAT CCTAAAGATG ACCGGGAGGC TCAGAGCATC
    TGTGAGCGGG TAACTCCCCG GTTATCTCAT GCCAACTCAG CAGTGGTGCT TTCAGCAGTA
    AAAGTCCTAA TGAAATTTTT AGAGTTGCTG CCCAAGGACT CTGACTACTA CAGTATGCTG
    CTGAAGAAGT TGGCCCCTCC ACTTGTCACT CTGCTGTCTG GGGAGCCAGA GGTGCAATAC
    GTCGCCCTGA GGAACATCAA CCTAATTGTT CAGAAAAGGC CTGAAATCTT GAAGCAGGAA
    ATAAAAGTTT TCTTTGTGAA GTACAATGAT CCCATCTATG TGAAACTAGA GAAGTTGGAC
    ATCATGATTC GTTTGGCATC CCAAGCCAAC ATTGCTCAGG TTCTGGCTGA ACTGAAAGAG
    TATGCCACAG AGGTGGATGT TGACTTTGTG CGCAAAGCTG TGCGAGCCAT TGGGCGGTGT
    GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CGTTGCTTGA TCTCATCCAA
    ACCAAAGTAA ATTATGTGGT CCAAGAGGCG ATTGTTGTCA TCAGGGACAT TTTCCGCAAA
    TACCCCAACA AATACGAAAG TATCATTGCC ACACTGTGTG AGAACTTAGA TTCTCTGGAT
    GAGCCAGACG CTCGAGCAGC TATGATTTGG ATTGTGGGAG AGTATGCTGA AAGAATTGAC
    AATGCTGATG AGTTACTAGA GAGCTTCCTG GAGGGTTTTC ATGATGAAAG CACCCAAGTG
    CAGCTCACTC TGCTCACTGC CATAGTGAAG CTGTTTCTCA AGAAACCGTC AGAAACACAG
    GAGCTGGTAC AGCAGGTCTT GAGTTTGGCA ACACAGGATT CTGATAATCC TGACCTTCGA
    GACCGGGGCT ATATTTATTG GCGCCTTCTC TCAACTGATC CTGTCACAGC TAAAGAAGTA
    GTCTTGTCTG AAAAGCCACT GATTTCTGAG GAGACAGATC TTATTGAACC AACTCTACTG
    GATGAGCTAA TCTGCCACAT TGGTTCTTTG GCCTCAGTGT ACCATAAGCC GCCCAATGCT
    TTTGTGGAAG GAAGTCATGG AATCCATCGC AAACACTTGC CAATACATCA TGGGAGCACT
    GATGCAGGTG ACAGCCCTGT TGGCGCCGCC ACTGCCACCA ACCTGGAGCA GCCTCAGGTT
    ATTCCCTCCC AGGGTGACCT TCTGGGGGAT CTTTTAAACC TTGACCTTGG TCCCCCAGTC
    AATGTGCCAC AGGTATCTTC CATGCAGATG GGAGCAGTGG ATCTCTTGGG AGGAGGCCTA
    GATAGTCTGC TTGGCAGTGA CCTTGGCGGG GGCATTGGAG GAAGTCCGGC AGTGGGACAG
    TCCTTCATCC CCTCATCAGT GCCTGCAACC TTTGCTCCCT CCCCTACTCC TGCTGTGGTC
    AGCAGTGGTC TGAATGACCT GTTTGAACTC TCCACGGGGA TAGGCATGGC ACCTGGTGGA
    TATGTGGCTC CTAAGGCTGT CTGGCTGCCT GCGGTAAAGG CTAAAGGCCT GGAGATTTCA
    GGAACATTTA CTCACCGCCA AGGGCACATC TATATGGAAA TGAACTTCAC CAACAAAGCT
    CTGCAGCACA TGACAGACTT CGCAATTCAG TTTAACAAGA ATAGCTTTGG TGTCATCCCT
    AGCACTCCTC TGGCCATCCA TACGCCACTG ATGCCAAACC AGAGCATTGA TGTCTCCCTG
    CCCCTCAACA CCTTGGGCCC TGTCATGAAG ATGGAGCCTC TGAACAACCT GCAGGTGGCT
    GTGAAAAACA ATATTGATGT CTTCTACTTC AGCTGCCTCA TCCCACTCAA CGTGCTTTTT
    GTAGAAGATG GCAAAATGGA GCGCCAGGTC TTCCTTGCAA CATGGAAGGA TATTCCCAAT
    GAAAATGAAC TTCAGTTTCA GATTAAGGAA TGTCATTTAA ATGCTGACAC TGTTTCCAGC
    AAGTTGCAAA ACAACAATGT TTATACTATT GCCAAGAGGA ATGTGGAAGG GCAGGACATG
    CTGTACCAAT CCCTGAAGCT CACTAATGGC ATTTGGATTT TGGCCGAGCT ACGTATCCAA
    CCAGGAAACC CCAATTACAC GCTGTCGCTG AAGTGTAGAG CTCCCGAGGT CTCCCAGTAC
    ATCTATCAGG TCTACGACAG CATTTTGAAA AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006056283.1
    CDS152..3007
    Translation

    Target ORF information:

    RefSeq Version XM_006056221.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis adaptor-related protein complex 2, beta 1 subunit (AP2B1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006056221.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGACTGACT CCAAGTACTT CACAACCAAT AAAAAGGGAG AAATCTTTGA ATTAAAGGCT 
    GAACTCAACA ATGAAAAGAA AGAAAAGAGG AAGGAGGCTG TGAAGAAAGT GATTGCTGCT
    ATGACTGTGG GGAAAGACGT TAGCTCTCTT TTTCCAGATG TAGTGAACTG TATGCAGACG
    GATAATCTGG AACTGAAGAA GCTTGTGTAT CTCTACTTGA TGAACTATGC CAAGAGTCAG
    CCAGACATGG CCATCATGGC TGTCAACAGC TTTGTGAAGG ATTGTGAAGA TCCCAATCCT
    CTGATTCGAG CCTTGGCAGT CAGAACCATG GGGTGCATCC GGGTGGACAA GATTACAGAG
    TATCTCTGTG AGCCCCTCCG CAAGTGCTTA AAGGATGAAG ATCCCTACGT CCGGAAGACA
    GCAGCAGTCT GTGTGGCAAA ACTCCATGAC ATCAATGCCC AGATGGTGGA AGATCAGGGA
    TTTCTGGATT CTCTGCGGGA TCTCATAGCA GATTCAAATC CAATGGTGGT GGCTAATGCT
    GTAGCAGCAC TATCTGAAAT CAGTGAGTCT CACCCCAACA GCAACTTACT CGATCTGAAT
    CCACAGAACA TTAATAAGCT ACTGACAGCC CTGAATGAGT GCACCGAATG GGGCCAGATT
    TTCATCCTGG ACTGCTTATC TAATTACAAT CCTAAAGATG ACCGGGAGGC TCAGAGCATC
    TGTGAGCGGG TAACTCCCCG GTTATCTCAT GCCAACTCAG CAGTGGTGCT TTCAGCAGTA
    AAAGTCCTAA TGAAATTTTT AGAGTTGCTG CCCAAGGACT CTGACTACTA CAGTATGCTG
    CTGAAGAAGT TGGCCCCTCC ACTTGTCACT CTGCTGTCTG GGGAGCCAGA GGTGCAATAC
    GTCGCCCTGA GGAACATCAA CCTAATTGTT CAGAAAAGGC CTGAAATCTT GAAGCAGGAA
    ATAAAAGTTT TCTTTGTGAA GTACAATGAT CCCATCTATG TGAAACTAGA GAAGTTGGAC
    ATCATGATTC GTTTGGCATC CCAAGCCAAC ATTGCTCAGG TTCTGGCTGA ACTGAAAGAG
    TATGCCACAG AGGTGGATGT TGACTTTGTG CGCAAAGCTG TGCGAGCCAT TGGGCGGTGT
    GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CGTTGCTTGA TCTCATCCAA
    ACCAAAGTAA ATTATGTGGT CCAAGAGGCG ATTGTTGTCA TCAGGGACAT TTTCCGCAAA
    TACCCCAACA AATACGAAAG TATCATTGCC ACACTGTGTG AGAACTTAGA TTCTCTGGAT
    GAGCCAGACG CTCGAGCAGC TATGATTTGG ATTGTGGGAG AGTATGCTGA AAGAATTGAC
    AATGCTGATG AGTTACTAGA GAGCTTCCTG GAGGGTTTTC ATGATGAAAG CACCCAAGTG
    CAGCTCACTC TGCTCACTGC CATAGTGAAG CTGTTTCTCA AGAAACCGTC AGAAACACAG
    GAGCTGGTAC AGCAGGTCTT GAGTTTGGCA ACACAGGATT CTGATAATCC TGACCTTCGA
    GACCGGGGCT ATATTTATTG GCGCCTTCTC TCAACTGATC CTGTCACAGC TAAAGAAGTA
    GTCTTGTCTG AAAAGCCACT GATTTCTGAG GAGACAGATC TTATTGAACC AACTCTACTG
    GATGAGCTAA TCTGCCACAT TGGTTCTTTG GCCTCAGTGT ACCATAAGCC GCCCAATGCT
    TTTGTGGAAG GAAGTCATGG AATCCATCGC AAACACTTGC CAATACATCA TGGGAGCACT
    GATGCAGGTG ACAGCCCTGT TGGCGCCGCC ACTGCCACCA ACCTGGAGCA GCCTCAGGTT
    ATTCCCTCCC AGGGTGACCT TCTGGGGGAT CTTTTAAACC TTGACCTTGG TCCCCCAGTC
    AATGTGCCAC AGGTATCTTC CATGCAGATG GGAGCAGTGG ATCTCTTGGG AGGAGGCCTA
    GATAGTCTGC TTGGCAGTGA CCTTGGCGGG GGCATTGGAG GAAGTCCGGC AGTGGGACAG
    TCCTTCATCC CCTCATCAGT GCCTGCAACC TTTGCTCCCT CCCCTACTCC TGCTGTGGTC
    AGCAGTGGTC TGAATGACCT GTTTGAACTC TCCACGGGGA TAGGCATGGC ACCTGGTGGA
    TATGTGGCTC CTAAGGCTGT CTGGCTGCCT GCGGTAAAGG CTAAAGGCCT GGAGATTTCA
    GGAACATTTA CTCACCGCCA AGGGCACATC TATATGGAAA TGAACTTCAC CAACAAAGCT
    CTGCAGCACA TGACAGACTT CGCAATTCAG TTTAACAAGA ATAGCTTTGG TGTCATCCCT
    AGCACTCCTC TGGCCATCCA TACGCCACTG ATGCCAAACC AGAGCATTGA TGTCTCCCTG
    CCCCTCAACA CCTTGGGCCC TGTCATGAAG ATGGAGCCTC TGAACAACCT GCAGGTGGCT
    GTGAAAAACA ATATTGATGT CTTCTACTTC AGCTGCCTCA TCCCACTCAA CGTGCTTTTT
    GTAGAAGATG GCAAAATGGA GCGCCAGGTC TTCCTTGCAA CATGGAAGGA TATTCCCAAT
    GAAAATGAAC TTCAGTTTCA GATTAAGGAA TGTCATTTAA ATGCTGACAC TGTTTCCAGC
    AAGTTGCAAA ACAACAATGT TTATACTATT GCCAAGAGGA ATGTGGAAGG GCAGGACATG
    CTGTACCAAT CCCTGAAGCT CACTAATGGC ATTTGGATTT TGGCCGAGCT ACGTATCCAA
    CCAGGAAACC CCAATTACAC GCTGTCGCTG AAGTGTAGAG CTCCCGAGGT CTCCCAGTAC
    ATCTATCAGG TCTACGACAG CATTTTGAAA AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu13375
    Clone ID Related Accession (Same CDS sequence) XM_006056222.1 , XM_006056221.1 , XM_006056221.2 , XM_025281091.1 , XM_025281092.1 , XM_006056222.2
    Accession Version XM_006056221.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product AP-2 complex subunit beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006056221.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGACTGACT CCAAGTACTT CACAACCAAT AAAAAGGGAG AAATCTTTGA ATTAAAGGCT 
    GAACTCAACA ATGAAAAGAA AGAAAAGAGG AAGGAGGCTG TGAAGAAAGT GATTGCTGCT
    ATGACTGTGG GGAAAGACGT TAGCTCTCTT TTTCCAGATG TAGTGAACTG TATGCAGACG
    GATAATCTGG AACTGAAGAA GCTTGTGTAT CTCTACTTGA TGAACTATGC CAAGAGTCAG
    CCAGACATGG CCATCATGGC TGTCAACAGC TTTGTGAAGG ATTGTGAAGA TCCCAATCCT
    CTGATTCGAG CCTTGGCAGT CAGAACCATG GGGTGCATCC GGGTGGACAA GATTACAGAG
    TATCTCTGTG AGCCCCTCCG CAAGTGCTTA AAGGATGAAG ATCCCTACGT CCGGAAGACA
    GCAGCAGTCT GTGTGGCAAA ACTCCATGAC ATCAATGCCC AGATGGTGGA AGATCAGGGA
    TTTCTGGATT CTCTGCGGGA TCTCATAGCA GATTCAAATC CAATGGTGGT GGCTAATGCT
    GTAGCAGCAC TATCTGAAAT CAGTGAGTCT CACCCCAACA GCAACTTACT CGATCTGAAT
    CCACAGAACA TTAATAAGCT ACTGACAGCC CTGAATGAGT GCACCGAATG GGGCCAGATT
    TTCATCCTGG ACTGCTTATC TAATTACAAT CCTAAAGATG ACCGGGAGGC TCAGAGCATC
    TGTGAGCGGG TAACTCCCCG GTTATCTCAT GCCAACTCAG CAGTGGTGCT TTCAGCAGTA
    AAAGTCCTAA TGAAATTTTT AGAGTTGCTG CCCAAGGACT CTGACTACTA CAGTATGCTG
    CTGAAGAAGT TGGCCCCTCC ACTTGTCACT CTGCTGTCTG GGGAGCCAGA GGTGCAATAC
    GTCGCCCTGA GGAACATCAA CCTAATTGTT CAGAAAAGGC CTGAAATCTT GAAGCAGGAA
    ATAAAAGTTT TCTTTGTGAA GTACAATGAT CCCATCTATG TGAAACTAGA GAAGTTGGAC
    ATCATGATTC GTTTGGCATC CCAAGCCAAC ATTGCTCAGG TTCTGGCTGA ACTGAAAGAG
    TATGCCACAG AGGTGGATGT TGACTTTGTG CGCAAAGCTG TGCGAGCCAT TGGGCGGTGT
    GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CGTTGCTTGA TCTCATCCAA
    ACCAAAGTAA ATTATGTGGT CCAAGAGGCG ATTGTTGTCA TCAGGGACAT TTTCCGCAAA
    TACCCCAACA AATACGAAAG TATCATTGCC ACACTGTGTG AGAACTTAGA TTCTCTGGAT
    GAGCCAGACG CTCGAGCAGC TATGATTTGG ATTGTGGGAG AGTATGCTGA AAGAATTGAC
    AATGCTGATG AGTTACTAGA GAGCTTCCTG GAGGGTTTTC ATGATGAAAG CACCCAAGTG
    CAGCTCACTC TGCTCACTGC CATAGTGAAG CTGTTTCTCA AGAAACCGTC AGAAACACAG
    GAGCTGGTAC AGCAGGTCTT GAGTTTGGCA ACACAGGATT CTGATAATCC TGACCTTCGA
    GACCGGGGCT ATATTTATTG GCGCCTTCTC TCAACTGATC CTGTCACAGC TAAAGAAGTA
    GTCTTGTCTG AAAAGCCACT GATTTCTGAG GAGACAGATC TTATTGAACC AACTCTACTG
    GATGAGCTAA TCTGCCACAT TGGTTCTTTG GCCTCAGTGT ACCATAAGCC GCCCAATGCT
    TTTGTGGAAG GAAGTCATGG AATCCATCGC AAACACTTGC CAATACATCA TGGGAGCACT
    GATGCAGGTG ACAGCCCTGT TGGCGCCGCC ACTGCCACCA ACCTGGAGCA GCCTCAGGTT
    ATTCCCTCCC AGGGTGACCT TCTGGGGGAT CTTTTAAACC TTGACCTTGG TCCCCCAGTC
    AATGTGCCAC AGGTATCTTC CATGCAGATG GGAGCAGTGG ATCTCTTGGG AGGAGGCCTA
    GATAGTCTGC TTGGCAGTGA CCTTGGCGGG GGCATTGGAG GAAGTCCGGC AGTGGGACAG
    TCCTTCATCC CCTCATCAGT GCCTGCAACC TTTGCTCCCT CCCCTACTCC TGCTGTGGTC
    AGCAGTGGTC TGAATGACCT GTTTGAACTC TCCACGGGGA TAGGCATGGC ACCTGGTGGA
    TATGTGGCTC CTAAGGCTGT CTGGCTGCCT GCGGTAAAGG CTAAAGGCCT GGAGATTTCA
    GGAACATTTA CTCACCGCCA AGGGCACATC TATATGGAAA TGAACTTCAC CAACAAAGCT
    CTGCAGCACA TGACAGACTT CGCAATTCAG TTTAACAAGA ATAGCTTTGG TGTCATCCCT
    AGCACTCCTC TGGCCATCCA TACGCCACTG ATGCCAAACC AGAGCATTGA TGTCTCCCTG
    CCCCTCAACA CCTTGGGCCC TGTCATGAAG ATGGAGCCTC TGAACAACCT GCAGGTGGCT
    GTGAAAAACA ATATTGATGT CTTCTACTTC AGCTGCCTCA TCCCACTCAA CGTGCTTTTT
    GTAGAAGATG GCAAAATGGA GCGCCAGGTC TTCCTTGCAA CATGGAAGGA TATTCCCAAT
    GAAAATGAAC TTCAGTTTCA GATTAAGGAA TGTCATTTAA ATGCTGACAC TGTTTCCAGC
    AAGTTGCAAA ACAACAATGT TTATACTATT GCCAAGAGGA ATGTGGAAGG GCAGGACATG
    CTGTACCAAT CCCTGAAGCT CACTAATGGC ATTTGGATTT TGGCCGAGCT ACGTATCCAA
    CCAGGAAACC CCAATTACAC GCTGTCGCTG AAGTGTAGAG CTCCCGAGGT CTCCCAGTAC
    ATCTATCAGG TCTACGACAG CATTTTGAAA AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006056283.1
    CDS175..3030
    Translation

    Target ORF information:

    RefSeq Version XM_006056221.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis adaptor related protein complex 2 subunit beta 1 (AP2B1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006056221.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGACTGACT CCAAGTACTT CACAACCAAT AAAAAGGGAG AAATCTTTGA ATTAAAGGCT 
    GAACTCAACA ATGAAAAGAA AGAAAAGAGG AAGGAGGCTG TGAAGAAAGT GATTGCTGCT
    ATGACTGTGG GGAAAGACGT TAGCTCTCTT TTTCCAGATG TAGTGAACTG TATGCAGACG
    GATAATCTGG AACTGAAGAA GCTTGTGTAT CTCTACTTGA TGAACTATGC CAAGAGTCAG
    CCAGACATGG CCATCATGGC TGTCAACAGC TTTGTGAAGG ATTGTGAAGA TCCCAATCCT
    CTGATTCGAG CCTTGGCAGT CAGAACCATG GGGTGCATCC GGGTGGACAA GATTACAGAG
    TATCTCTGTG AGCCCCTCCG CAAGTGCTTA AAGGATGAAG ATCCCTACGT CCGGAAGACA
    GCAGCAGTCT GTGTGGCAAA ACTCCATGAC ATCAATGCCC AGATGGTGGA AGATCAGGGA
    TTTCTGGATT CTCTGCGGGA TCTCATAGCA GATTCAAATC CAATGGTGGT GGCTAATGCT
    GTAGCAGCAC TATCTGAAAT CAGTGAGTCT CACCCCAACA GCAACTTACT CGATCTGAAT
    CCACAGAACA TTAATAAGCT ACTGACAGCC CTGAATGAGT GCACCGAATG GGGCCAGATT
    TTCATCCTGG ACTGCTTATC TAATTACAAT CCTAAAGATG ACCGGGAGGC TCAGAGCATC
    TGTGAGCGGG TAACTCCCCG GTTATCTCAT GCCAACTCAG CAGTGGTGCT TTCAGCAGTA
    AAAGTCCTAA TGAAATTTTT AGAGTTGCTG CCCAAGGACT CTGACTACTA CAGTATGCTG
    CTGAAGAAGT TGGCCCCTCC ACTTGTCACT CTGCTGTCTG GGGAGCCAGA GGTGCAATAC
    GTCGCCCTGA GGAACATCAA CCTAATTGTT CAGAAAAGGC CTGAAATCTT GAAGCAGGAA
    ATAAAAGTTT TCTTTGTGAA GTACAATGAT CCCATCTATG TGAAACTAGA GAAGTTGGAC
    ATCATGATTC GTTTGGCATC CCAAGCCAAC ATTGCTCAGG TTCTGGCTGA ACTGAAAGAG
    TATGCCACAG AGGTGGATGT TGACTTTGTG CGCAAAGCTG TGCGAGCCAT TGGGCGGTGT
    GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CGTTGCTTGA TCTCATCCAA
    ACCAAAGTAA ATTATGTGGT CCAAGAGGCG ATTGTTGTCA TCAGGGACAT TTTCCGCAAA
    TACCCCAACA AATACGAAAG TATCATTGCC ACACTGTGTG AGAACTTAGA TTCTCTGGAT
    GAGCCAGACG CTCGAGCAGC TATGATTTGG ATTGTGGGAG AGTATGCTGA AAGAATTGAC
    AATGCTGATG AGTTACTAGA GAGCTTCCTG GAGGGTTTTC ATGATGAAAG CACCCAAGTG
    CAGCTCACTC TGCTCACTGC CATAGTGAAG CTGTTTCTCA AGAAACCGTC AGAAACACAG
    GAGCTGGTAC AGCAGGTCTT GAGTTTGGCA ACACAGGATT CTGATAATCC TGACCTTCGA
    GACCGGGGCT ATATTTATTG GCGCCTTCTC TCAACTGATC CTGTCACAGC TAAAGAAGTA
    GTCTTGTCTG AAAAGCCACT GATTTCTGAG GAGACAGATC TTATTGAACC AACTCTACTG
    GATGAGCTAA TCTGCCACAT TGGTTCTTTG GCCTCAGTGT ACCATAAGCC GCCCAATGCT
    TTTGTGGAAG GAAGTCATGG AATCCATCGC AAACACTTGC CAATACATCA TGGGAGCACT
    GATGCAGGTG ACAGCCCTGT TGGCGCCGCC ACTGCCACCA ACCTGGAGCA GCCTCAGGTT
    ATTCCCTCCC AGGGTGACCT TCTGGGGGAT CTTTTAAACC TTGACCTTGG TCCCCCAGTC
    AATGTGCCAC AGGTATCTTC CATGCAGATG GGAGCAGTGG ATCTCTTGGG AGGAGGCCTA
    GATAGTCTGC TTGGCAGTGA CCTTGGCGGG GGCATTGGAG GAAGTCCGGC AGTGGGACAG
    TCCTTCATCC CCTCATCAGT GCCTGCAACC TTTGCTCCCT CCCCTACTCC TGCTGTGGTC
    AGCAGTGGTC TGAATGACCT GTTTGAACTC TCCACGGGGA TAGGCATGGC ACCTGGTGGA
    TATGTGGCTC CTAAGGCTGT CTGGCTGCCT GCGGTAAAGG CTAAAGGCCT GGAGATTTCA
    GGAACATTTA CTCACCGCCA AGGGCACATC TATATGGAAA TGAACTTCAC CAACAAAGCT
    CTGCAGCACA TGACAGACTT CGCAATTCAG TTTAACAAGA ATAGCTTTGG TGTCATCCCT
    AGCACTCCTC TGGCCATCCA TACGCCACTG ATGCCAAACC AGAGCATTGA TGTCTCCCTG
    CCCCTCAACA CCTTGGGCCC TGTCATGAAG ATGGAGCCTC TGAACAACCT GCAGGTGGCT
    GTGAAAAACA ATATTGATGT CTTCTACTTC AGCTGCCTCA TCCCACTCAA CGTGCTTTTT
    GTAGAAGATG GCAAAATGGA GCGCCAGGTC TTCCTTGCAA CATGGAAGGA TATTCCCAAT
    GAAAATGAAC TTCAGTTTCA GATTAAGGAA TGTCATTTAA ATGCTGACAC TGTTTCCAGC
    AAGTTGCAAA ACAACAATGT TTATACTATT GCCAAGAGGA ATGTGGAAGG GCAGGACATG
    CTGTACCAAT CCCTGAAGCT CACTAATGGC ATTTGGATTT TGGCCGAGCT ACGTATCCAA
    CCAGGAAACC CCAATTACAC GCTGTCGCTG AAGTGTAGAG CTCCCGAGGT CTCCCAGTAC
    ATCTATCAGG TCTACGACAG CATTTTGAAA AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu13375
    Clone ID Related Accession (Same CDS sequence) XM_006056222.1 , XM_006056221.1 , XM_006056221.2 , XM_025281091.1 , XM_025281092.1 , XM_006056222.2
    Accession Version XM_025281091.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product AP-2 complex subunit beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGACTGACT CCAAGTACTT CACAACCAAT AAAAAGGGAG AAATCTTTGA ATTAAAGGCT 
    GAACTCAACA ATGAAAAGAA AGAAAAGAGG AAGGAGGCTG TGAAGAAAGT GATTGCTGCT
    ATGACTGTGG GGAAAGACGT TAGCTCTCTT TTTCCAGATG TAGTGAACTG TATGCAGACG
    GATAATCTGG AACTGAAGAA GCTTGTGTAT CTCTACTTGA TGAACTATGC CAAGAGTCAG
    CCAGACATGG CCATCATGGC TGTCAACAGC TTTGTGAAGG ATTGTGAAGA TCCCAATCCT
    CTGATTCGAG CCTTGGCAGT CAGAACCATG GGGTGCATCC GGGTGGACAA GATTACAGAG
    TATCTCTGTG AGCCCCTCCG CAAGTGCTTA AAGGATGAAG ATCCCTACGT CCGGAAGACA
    GCAGCAGTCT GTGTGGCAAA ACTCCATGAC ATCAATGCCC AGATGGTGGA AGATCAGGGA
    TTTCTGGATT CTCTGCGGGA TCTCATAGCA GATTCAAATC CAATGGTGGT GGCTAATGCT
    GTAGCAGCAC TATCTGAAAT CAGTGAGTCT CACCCCAACA GCAACTTACT CGATCTGAAT
    CCACAGAACA TTAATAAGCT ACTGACAGCC CTGAATGAGT GCACCGAATG GGGCCAGATT
    TTCATCCTGG ACTGCTTATC TAATTACAAT CCTAAAGATG ACCGGGAGGC TCAGAGCATC
    TGTGAGCGGG TAACTCCCCG GTTATCTCAT GCCAACTCAG CAGTGGTGCT TTCAGCAGTA
    AAAGTCCTAA TGAAATTTTT AGAGTTGCTG CCCAAGGACT CTGACTACTA CAGTATGCTG
    CTGAAGAAGT TGGCCCCTCC ACTTGTCACT CTGCTGTCTG GGGAGCCAGA GGTGCAATAC
    GTCGCCCTGA GGAACATCAA CCTAATTGTT CAGAAAAGGC CTGAAATCTT GAAGCAGGAA
    ATAAAAGTTT TCTTTGTGAA GTACAATGAT CCCATCTATG TGAAACTAGA GAAGTTGGAC
    ATCATGATTC GTTTGGCATC CCAAGCCAAC ATTGCTCAGG TTCTGGCTGA ACTGAAAGAG
    TATGCCACAG AGGTGGATGT TGACTTTGTG CGCAAAGCTG TGCGAGCCAT TGGGCGGTGT
    GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CGTTGCTTGA TCTCATCCAA
    ACCAAAGTAA ATTATGTGGT CCAAGAGGCG ATTGTTGTCA TCAGGGACAT TTTCCGCAAA
    TACCCCAACA AATACGAAAG TATCATTGCC ACACTGTGTG AGAACTTAGA TTCTCTGGAT
    GAGCCAGACG CTCGAGCAGC TATGATTTGG ATTGTGGGAG AGTATGCTGA AAGAATTGAC
    AATGCTGATG AGTTACTAGA GAGCTTCCTG GAGGGTTTTC ATGATGAAAG CACCCAAGTG
    CAGCTCACTC TGCTCACTGC CATAGTGAAG CTGTTTCTCA AGAAACCGTC AGAAACACAG
    GAGCTGGTAC AGCAGGTCTT GAGTTTGGCA ACACAGGATT CTGATAATCC TGACCTTCGA
    GACCGGGGCT ATATTTATTG GCGCCTTCTC TCAACTGATC CTGTCACAGC TAAAGAAGTA
    GTCTTGTCTG AAAAGCCACT GATTTCTGAG GAGACAGATC TTATTGAACC AACTCTACTG
    GATGAGCTAA TCTGCCACAT TGGTTCTTTG GCCTCAGTGT ACCATAAGCC GCCCAATGCT
    TTTGTGGAAG GAAGTCATGG AATCCATCGC AAACACTTGC CAATACATCA TGGGAGCACT
    GATGCAGGTG ACAGCCCTGT TGGCGCCGCC ACTGCCACCA ACCTGGAGCA GCCTCAGGTT
    ATTCCCTCCC AGGGTGACCT TCTGGGGGAT CTTTTAAACC TTGACCTTGG TCCCCCAGTC
    AATGTGCCAC AGGTATCTTC CATGCAGATG GGAGCAGTGG ATCTCTTGGG AGGAGGCCTA
    GATAGTCTGC TTGGCAGTGA CCTTGGCGGG GGCATTGGAG GAAGTCCGGC AGTGGGACAG
    TCCTTCATCC CCTCATCAGT GCCTGCAACC TTTGCTCCCT CCCCTACTCC TGCTGTGGTC
    AGCAGTGGTC TGAATGACCT GTTTGAACTC TCCACGGGGA TAGGCATGGC ACCTGGTGGA
    TATGTGGCTC CTAAGGCTGT CTGGCTGCCT GCGGTAAAGG CTAAAGGCCT GGAGATTTCA
    GGAACATTTA CTCACCGCCA AGGGCACATC TATATGGAAA TGAACTTCAC CAACAAAGCT
    CTGCAGCACA TGACAGACTT CGCAATTCAG TTTAACAAGA ATAGCTTTGG TGTCATCCCT
    AGCACTCCTC TGGCCATCCA TACGCCACTG ATGCCAAACC AGAGCATTGA TGTCTCCCTG
    CCCCTCAACA CCTTGGGCCC TGTCATGAAG ATGGAGCCTC TGAACAACCT GCAGGTGGCT
    GTGAAAAACA ATATTGATGT CTTCTACTTC AGCTGCCTCA TCCCACTCAA CGTGCTTTTT
    GTAGAAGATG GCAAAATGGA GCGCCAGGTC TTCCTTGCAA CATGGAAGGA TATTCCCAAT
    GAAAATGAAC TTCAGTTTCA GATTAAGGAA TGTCATTTAA ATGCTGACAC TGTTTCCAGC
    AAGTTGCAAA ACAACAATGT TTATACTATT GCCAAGAGGA ATGTGGAAGG GCAGGACATG
    CTGTACCAAT CCCTGAAGCT CACTAATGGC ATTTGGATTT TGGCCGAGCT ACGTATCCAA
    CCAGGAAACC CCAATTACAC GCTGTCGCTG AAGTGTAGAG CTCCCGAGGT CTCCCAGTAC
    ATCTATCAGG TCTACGACAG CATTTTGAAA AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025136876.1
    CDS141..2996
    Translation

    Target ORF information:

    RefSeq Version XM_025281091.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis adaptor related protein complex 2 subunit beta 1 (AP2B1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025281091.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGACTGACT CCAAGTACTT CACAACCAAT AAAAAGGGAG AAATCTTTGA ATTAAAGGCT 
    GAACTCAACA ATGAAAAGAA AGAAAAGAGG AAGGAGGCTG TGAAGAAAGT GATTGCTGCT
    ATGACTGTGG GGAAAGACGT TAGCTCTCTT TTTCCAGATG TAGTGAACTG TATGCAGACG
    GATAATCTGG AACTGAAGAA GCTTGTGTAT CTCTACTTGA TGAACTATGC CAAGAGTCAG
    CCAGACATGG CCATCATGGC TGTCAACAGC TTTGTGAAGG ATTGTGAAGA TCCCAATCCT
    CTGATTCGAG CCTTGGCAGT CAGAACCATG GGGTGCATCC GGGTGGACAA GATTACAGAG
    TATCTCTGTG AGCCCCTCCG CAAGTGCTTA AAGGATGAAG ATCCCTACGT CCGGAAGACA
    GCAGCAGTCT GTGTGGCAAA ACTCCATGAC ATCAATGCCC AGATGGTGGA AGATCAGGGA
    TTTCTGGATT CTCTGCGGGA TCTCATAGCA GATTCAAATC CAATGGTGGT GGCTAATGCT
    GTAGCAGCAC TATCTGAAAT CAGTGAGTCT CACCCCAACA GCAACTTACT CGATCTGAAT
    CCACAGAACA TTAATAAGCT ACTGACAGCC CTGAATGAGT GCACCGAATG GGGCCAGATT
    TTCATCCTGG ACTGCTTATC TAATTACAAT CCTAAAGATG ACCGGGAGGC TCAGAGCATC
    TGTGAGCGGG TAACTCCCCG GTTATCTCAT GCCAACTCAG CAGTGGTGCT TTCAGCAGTA
    AAAGTCCTAA TGAAATTTTT AGAGTTGCTG CCCAAGGACT CTGACTACTA CAGTATGCTG
    CTGAAGAAGT TGGCCCCTCC ACTTGTCACT CTGCTGTCTG GGGAGCCAGA GGTGCAATAC
    GTCGCCCTGA GGAACATCAA CCTAATTGTT CAGAAAAGGC CTGAAATCTT GAAGCAGGAA
    ATAAAAGTTT TCTTTGTGAA GTACAATGAT CCCATCTATG TGAAACTAGA GAAGTTGGAC
    ATCATGATTC GTTTGGCATC CCAAGCCAAC ATTGCTCAGG TTCTGGCTGA ACTGAAAGAG
    TATGCCACAG AGGTGGATGT TGACTTTGTG CGCAAAGCTG TGCGAGCCAT TGGGCGGTGT
    GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CGTTGCTTGA TCTCATCCAA
    ACCAAAGTAA ATTATGTGGT CCAAGAGGCG ATTGTTGTCA TCAGGGACAT TTTCCGCAAA
    TACCCCAACA AATACGAAAG TATCATTGCC ACACTGTGTG AGAACTTAGA TTCTCTGGAT
    GAGCCAGACG CTCGAGCAGC TATGATTTGG ATTGTGGGAG AGTATGCTGA AAGAATTGAC
    AATGCTGATG AGTTACTAGA GAGCTTCCTG GAGGGTTTTC ATGATGAAAG CACCCAAGTG
    CAGCTCACTC TGCTCACTGC CATAGTGAAG CTGTTTCTCA AGAAACCGTC AGAAACACAG
    GAGCTGGTAC AGCAGGTCTT GAGTTTGGCA ACACAGGATT CTGATAATCC TGACCTTCGA
    GACCGGGGCT ATATTTATTG GCGCCTTCTC TCAACTGATC CTGTCACAGC TAAAGAAGTA
    GTCTTGTCTG AAAAGCCACT GATTTCTGAG GAGACAGATC TTATTGAACC AACTCTACTG
    GATGAGCTAA TCTGCCACAT TGGTTCTTTG GCCTCAGTGT ACCATAAGCC GCCCAATGCT
    TTTGTGGAAG GAAGTCATGG AATCCATCGC AAACACTTGC CAATACATCA TGGGAGCACT
    GATGCAGGTG ACAGCCCTGT TGGCGCCGCC ACTGCCACCA ACCTGGAGCA GCCTCAGGTT
    ATTCCCTCCC AGGGTGACCT TCTGGGGGAT CTTTTAAACC TTGACCTTGG TCCCCCAGTC
    AATGTGCCAC AGGTATCTTC CATGCAGATG GGAGCAGTGG ATCTCTTGGG AGGAGGCCTA
    GATAGTCTGC TTGGCAGTGA CCTTGGCGGG GGCATTGGAG GAAGTCCGGC AGTGGGACAG
    TCCTTCATCC CCTCATCAGT GCCTGCAACC TTTGCTCCCT CCCCTACTCC TGCTGTGGTC
    AGCAGTGGTC TGAATGACCT GTTTGAACTC TCCACGGGGA TAGGCATGGC ACCTGGTGGA
    TATGTGGCTC CTAAGGCTGT CTGGCTGCCT GCGGTAAAGG CTAAAGGCCT GGAGATTTCA
    GGAACATTTA CTCACCGCCA AGGGCACATC TATATGGAAA TGAACTTCAC CAACAAAGCT
    CTGCAGCACA TGACAGACTT CGCAATTCAG TTTAACAAGA ATAGCTTTGG TGTCATCCCT
    AGCACTCCTC TGGCCATCCA TACGCCACTG ATGCCAAACC AGAGCATTGA TGTCTCCCTG
    CCCCTCAACA CCTTGGGCCC TGTCATGAAG ATGGAGCCTC TGAACAACCT GCAGGTGGCT
    GTGAAAAACA ATATTGATGT CTTCTACTTC AGCTGCCTCA TCCCACTCAA CGTGCTTTTT
    GTAGAAGATG GCAAAATGGA GCGCCAGGTC TTCCTTGCAA CATGGAAGGA TATTCCCAAT
    GAAAATGAAC TTCAGTTTCA GATTAAGGAA TGTCATTTAA ATGCTGACAC TGTTTCCAGC
    AAGTTGCAAA ACAACAATGT TTATACTATT GCCAAGAGGA ATGTGGAAGG GCAGGACATG
    CTGTACCAAT CCCTGAAGCT CACTAATGGC ATTTGGATTT TGGCCGAGCT ACGTATCCAA
    CCAGGAAACC CCAATTACAC GCTGTCGCTG AAGTGTAGAG CTCCCGAGGT CTCCCAGTAC
    ATCTATCAGG TCTACGACAG CATTTTGAAA AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu13375
    Clone ID Related Accession (Same CDS sequence) XM_006056222.1 , XM_006056221.1 , XM_006056221.2 , XM_025281091.1 , XM_025281092.1 , XM_006056222.2
    Accession Version XM_025281092.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product AP-2 complex subunit beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGACTGACT CCAAGTACTT CACAACCAAT AAAAAGGGAG AAATCTTTGA ATTAAAGGCT 
    GAACTCAACA ATGAAAAGAA AGAAAAGAGG AAGGAGGCTG TGAAGAAAGT GATTGCTGCT
    ATGACTGTGG GGAAAGACGT TAGCTCTCTT TTTCCAGATG TAGTGAACTG TATGCAGACG
    GATAATCTGG AACTGAAGAA GCTTGTGTAT CTCTACTTGA TGAACTATGC CAAGAGTCAG
    CCAGACATGG CCATCATGGC TGTCAACAGC TTTGTGAAGG ATTGTGAAGA TCCCAATCCT
    CTGATTCGAG CCTTGGCAGT CAGAACCATG GGGTGCATCC GGGTGGACAA GATTACAGAG
    TATCTCTGTG AGCCCCTCCG CAAGTGCTTA AAGGATGAAG ATCCCTACGT CCGGAAGACA
    GCAGCAGTCT GTGTGGCAAA ACTCCATGAC ATCAATGCCC AGATGGTGGA AGATCAGGGA
    TTTCTGGATT CTCTGCGGGA TCTCATAGCA GATTCAAATC CAATGGTGGT GGCTAATGCT
    GTAGCAGCAC TATCTGAAAT CAGTGAGTCT CACCCCAACA GCAACTTACT CGATCTGAAT
    CCACAGAACA TTAATAAGCT ACTGACAGCC CTGAATGAGT GCACCGAATG GGGCCAGATT
    TTCATCCTGG ACTGCTTATC TAATTACAAT CCTAAAGATG ACCGGGAGGC TCAGAGCATC
    TGTGAGCGGG TAACTCCCCG GTTATCTCAT GCCAACTCAG CAGTGGTGCT TTCAGCAGTA
    AAAGTCCTAA TGAAATTTTT AGAGTTGCTG CCCAAGGACT CTGACTACTA CAGTATGCTG
    CTGAAGAAGT TGGCCCCTCC ACTTGTCACT CTGCTGTCTG GGGAGCCAGA GGTGCAATAC
    GTCGCCCTGA GGAACATCAA CCTAATTGTT CAGAAAAGGC CTGAAATCTT GAAGCAGGAA
    ATAAAAGTTT TCTTTGTGAA GTACAATGAT CCCATCTATG TGAAACTAGA GAAGTTGGAC
    ATCATGATTC GTTTGGCATC CCAAGCCAAC ATTGCTCAGG TTCTGGCTGA ACTGAAAGAG
    TATGCCACAG AGGTGGATGT TGACTTTGTG CGCAAAGCTG TGCGAGCCAT TGGGCGGTGT
    GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CGTTGCTTGA TCTCATCCAA
    ACCAAAGTAA ATTATGTGGT CCAAGAGGCG ATTGTTGTCA TCAGGGACAT TTTCCGCAAA
    TACCCCAACA AATACGAAAG TATCATTGCC ACACTGTGTG AGAACTTAGA TTCTCTGGAT
    GAGCCAGACG CTCGAGCAGC TATGATTTGG ATTGTGGGAG AGTATGCTGA AAGAATTGAC
    AATGCTGATG AGTTACTAGA GAGCTTCCTG GAGGGTTTTC ATGATGAAAG CACCCAAGTG
    CAGCTCACTC TGCTCACTGC CATAGTGAAG CTGTTTCTCA AGAAACCGTC AGAAACACAG
    GAGCTGGTAC AGCAGGTCTT GAGTTTGGCA ACACAGGATT CTGATAATCC TGACCTTCGA
    GACCGGGGCT ATATTTATTG GCGCCTTCTC TCAACTGATC CTGTCACAGC TAAAGAAGTA
    GTCTTGTCTG AAAAGCCACT GATTTCTGAG GAGACAGATC TTATTGAACC AACTCTACTG
    GATGAGCTAA TCTGCCACAT TGGTTCTTTG GCCTCAGTGT ACCATAAGCC GCCCAATGCT
    TTTGTGGAAG GAAGTCATGG AATCCATCGC AAACACTTGC CAATACATCA TGGGAGCACT
    GATGCAGGTG ACAGCCCTGT TGGCGCCGCC ACTGCCACCA ACCTGGAGCA GCCTCAGGTT
    ATTCCCTCCC AGGGTGACCT TCTGGGGGAT CTTTTAAACC TTGACCTTGG TCCCCCAGTC
    AATGTGCCAC AGGTATCTTC CATGCAGATG GGAGCAGTGG ATCTCTTGGG AGGAGGCCTA
    GATAGTCTGC TTGGCAGTGA CCTTGGCGGG GGCATTGGAG GAAGTCCGGC AGTGGGACAG
    TCCTTCATCC CCTCATCAGT GCCTGCAACC TTTGCTCCCT CCCCTACTCC TGCTGTGGTC
    AGCAGTGGTC TGAATGACCT GTTTGAACTC TCCACGGGGA TAGGCATGGC ACCTGGTGGA
    TATGTGGCTC CTAAGGCTGT CTGGCTGCCT GCGGTAAAGG CTAAAGGCCT GGAGATTTCA
    GGAACATTTA CTCACCGCCA AGGGCACATC TATATGGAAA TGAACTTCAC CAACAAAGCT
    CTGCAGCACA TGACAGACTT CGCAATTCAG TTTAACAAGA ATAGCTTTGG TGTCATCCCT
    AGCACTCCTC TGGCCATCCA TACGCCACTG ATGCCAAACC AGAGCATTGA TGTCTCCCTG
    CCCCTCAACA CCTTGGGCCC TGTCATGAAG ATGGAGCCTC TGAACAACCT GCAGGTGGCT
    GTGAAAAACA ATATTGATGT CTTCTACTTC AGCTGCCTCA TCCCACTCAA CGTGCTTTTT
    GTAGAAGATG GCAAAATGGA GCGCCAGGTC TTCCTTGCAA CATGGAAGGA TATTCCCAAT
    GAAAATGAAC TTCAGTTTCA GATTAAGGAA TGTCATTTAA ATGCTGACAC TGTTTCCAGC
    AAGTTGCAAA ACAACAATGT TTATACTATT GCCAAGAGGA ATGTGGAAGG GCAGGACATG
    CTGTACCAAT CCCTGAAGCT CACTAATGGC ATTTGGATTT TGGCCGAGCT ACGTATCCAA
    CCAGGAAACC CCAATTACAC GCTGTCGCTG AAGTGTAGAG CTCCCGAGGT CTCCCAGTAC
    ATCTATCAGG TCTACGACAG CATTTTGAAA AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025136877.1
    CDS44..2899
    Translation

    Target ORF information:

    RefSeq Version XM_025281092.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis adaptor related protein complex 2 subunit beta 1 (AP2B1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025281092.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGACTGACT CCAAGTACTT CACAACCAAT AAAAAGGGAG AAATCTTTGA ATTAAAGGCT 
    GAACTCAACA ATGAAAAGAA AGAAAAGAGG AAGGAGGCTG TGAAGAAAGT GATTGCTGCT
    ATGACTGTGG GGAAAGACGT TAGCTCTCTT TTTCCAGATG TAGTGAACTG TATGCAGACG
    GATAATCTGG AACTGAAGAA GCTTGTGTAT CTCTACTTGA TGAACTATGC CAAGAGTCAG
    CCAGACATGG CCATCATGGC TGTCAACAGC TTTGTGAAGG ATTGTGAAGA TCCCAATCCT
    CTGATTCGAG CCTTGGCAGT CAGAACCATG GGGTGCATCC GGGTGGACAA GATTACAGAG
    TATCTCTGTG AGCCCCTCCG CAAGTGCTTA AAGGATGAAG ATCCCTACGT CCGGAAGACA
    GCAGCAGTCT GTGTGGCAAA ACTCCATGAC ATCAATGCCC AGATGGTGGA AGATCAGGGA
    TTTCTGGATT CTCTGCGGGA TCTCATAGCA GATTCAAATC CAATGGTGGT GGCTAATGCT
    GTAGCAGCAC TATCTGAAAT CAGTGAGTCT CACCCCAACA GCAACTTACT CGATCTGAAT
    CCACAGAACA TTAATAAGCT ACTGACAGCC CTGAATGAGT GCACCGAATG GGGCCAGATT
    TTCATCCTGG ACTGCTTATC TAATTACAAT CCTAAAGATG ACCGGGAGGC TCAGAGCATC
    TGTGAGCGGG TAACTCCCCG GTTATCTCAT GCCAACTCAG CAGTGGTGCT TTCAGCAGTA
    AAAGTCCTAA TGAAATTTTT AGAGTTGCTG CCCAAGGACT CTGACTACTA CAGTATGCTG
    CTGAAGAAGT TGGCCCCTCC ACTTGTCACT CTGCTGTCTG GGGAGCCAGA GGTGCAATAC
    GTCGCCCTGA GGAACATCAA CCTAATTGTT CAGAAAAGGC CTGAAATCTT GAAGCAGGAA
    ATAAAAGTTT TCTTTGTGAA GTACAATGAT CCCATCTATG TGAAACTAGA GAAGTTGGAC
    ATCATGATTC GTTTGGCATC CCAAGCCAAC ATTGCTCAGG TTCTGGCTGA ACTGAAAGAG
    TATGCCACAG AGGTGGATGT TGACTTTGTG CGCAAAGCTG TGCGAGCCAT TGGGCGGTGT
    GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CGTTGCTTGA TCTCATCCAA
    ACCAAAGTAA ATTATGTGGT CCAAGAGGCG ATTGTTGTCA TCAGGGACAT TTTCCGCAAA
    TACCCCAACA AATACGAAAG TATCATTGCC ACACTGTGTG AGAACTTAGA TTCTCTGGAT
    GAGCCAGACG CTCGAGCAGC TATGATTTGG ATTGTGGGAG AGTATGCTGA AAGAATTGAC
    AATGCTGATG AGTTACTAGA GAGCTTCCTG GAGGGTTTTC ATGATGAAAG CACCCAAGTG
    CAGCTCACTC TGCTCACTGC CATAGTGAAG CTGTTTCTCA AGAAACCGTC AGAAACACAG
    GAGCTGGTAC AGCAGGTCTT GAGTTTGGCA ACACAGGATT CTGATAATCC TGACCTTCGA
    GACCGGGGCT ATATTTATTG GCGCCTTCTC TCAACTGATC CTGTCACAGC TAAAGAAGTA
    GTCTTGTCTG AAAAGCCACT GATTTCTGAG GAGACAGATC TTATTGAACC AACTCTACTG
    GATGAGCTAA TCTGCCACAT TGGTTCTTTG GCCTCAGTGT ACCATAAGCC GCCCAATGCT
    TTTGTGGAAG GAAGTCATGG AATCCATCGC AAACACTTGC CAATACATCA TGGGAGCACT
    GATGCAGGTG ACAGCCCTGT TGGCGCCGCC ACTGCCACCA ACCTGGAGCA GCCTCAGGTT
    ATTCCCTCCC AGGGTGACCT TCTGGGGGAT CTTTTAAACC TTGACCTTGG TCCCCCAGTC
    AATGTGCCAC AGGTATCTTC CATGCAGATG GGAGCAGTGG ATCTCTTGGG AGGAGGCCTA
    GATAGTCTGC TTGGCAGTGA CCTTGGCGGG GGCATTGGAG GAAGTCCGGC AGTGGGACAG
    TCCTTCATCC CCTCATCAGT GCCTGCAACC TTTGCTCCCT CCCCTACTCC TGCTGTGGTC
    AGCAGTGGTC TGAATGACCT GTTTGAACTC TCCACGGGGA TAGGCATGGC ACCTGGTGGA
    TATGTGGCTC CTAAGGCTGT CTGGCTGCCT GCGGTAAAGG CTAAAGGCCT GGAGATTTCA
    GGAACATTTA CTCACCGCCA AGGGCACATC TATATGGAAA TGAACTTCAC CAACAAAGCT
    CTGCAGCACA TGACAGACTT CGCAATTCAG TTTAACAAGA ATAGCTTTGG TGTCATCCCT
    AGCACTCCTC TGGCCATCCA TACGCCACTG ATGCCAAACC AGAGCATTGA TGTCTCCCTG
    CCCCTCAACA CCTTGGGCCC TGTCATGAAG ATGGAGCCTC TGAACAACCT GCAGGTGGCT
    GTGAAAAACA ATATTGATGT CTTCTACTTC AGCTGCCTCA TCCCACTCAA CGTGCTTTTT
    GTAGAAGATG GCAAAATGGA GCGCCAGGTC TTCCTTGCAA CATGGAAGGA TATTCCCAAT
    GAAAATGAAC TTCAGTTTCA GATTAAGGAA TGTCATTTAA ATGCTGACAC TGTTTCCAGC
    AAGTTGCAAA ACAACAATGT TTATACTATT GCCAAGAGGA ATGTGGAAGG GCAGGACATG
    CTGTACCAAT CCCTGAAGCT CACTAATGGC ATTTGGATTT TGGCCGAGCT ACGTATCCAA
    CCAGGAAACC CCAATTACAC GCTGTCGCTG AAGTGTAGAG CTCCCGAGGT CTCCCAGTAC
    ATCTATCAGG TCTACGACAG CATTTTGAAA AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu13375
    Clone ID Related Accession (Same CDS sequence) XM_006056222.1 , XM_006056221.1 , XM_006056221.2 , XM_025281091.1 , XM_025281092.1 , XM_006056222.2
    Accession Version XM_006056222.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product AP-2 complex subunit beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006056222.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGACTGACT CCAAGTACTT CACAACCAAT AAAAAGGGAG AAATCTTTGA ATTAAAGGCT 
    GAACTCAACA ATGAAAAGAA AGAAAAGAGG AAGGAGGCTG TGAAGAAAGT GATTGCTGCT
    ATGACTGTGG GGAAAGACGT TAGCTCTCTT TTTCCAGATG TAGTGAACTG TATGCAGACG
    GATAATCTGG AACTGAAGAA GCTTGTGTAT CTCTACTTGA TGAACTATGC CAAGAGTCAG
    CCAGACATGG CCATCATGGC TGTCAACAGC TTTGTGAAGG ATTGTGAAGA TCCCAATCCT
    CTGATTCGAG CCTTGGCAGT CAGAACCATG GGGTGCATCC GGGTGGACAA GATTACAGAG
    TATCTCTGTG AGCCCCTCCG CAAGTGCTTA AAGGATGAAG ATCCCTACGT CCGGAAGACA
    GCAGCAGTCT GTGTGGCAAA ACTCCATGAC ATCAATGCCC AGATGGTGGA AGATCAGGGA
    TTTCTGGATT CTCTGCGGGA TCTCATAGCA GATTCAAATC CAATGGTGGT GGCTAATGCT
    GTAGCAGCAC TATCTGAAAT CAGTGAGTCT CACCCCAACA GCAACTTACT CGATCTGAAT
    CCACAGAACA TTAATAAGCT ACTGACAGCC CTGAATGAGT GCACCGAATG GGGCCAGATT
    TTCATCCTGG ACTGCTTATC TAATTACAAT CCTAAAGATG ACCGGGAGGC TCAGAGCATC
    TGTGAGCGGG TAACTCCCCG GTTATCTCAT GCCAACTCAG CAGTGGTGCT TTCAGCAGTA
    AAAGTCCTAA TGAAATTTTT AGAGTTGCTG CCCAAGGACT CTGACTACTA CAGTATGCTG
    CTGAAGAAGT TGGCCCCTCC ACTTGTCACT CTGCTGTCTG GGGAGCCAGA GGTGCAATAC
    GTCGCCCTGA GGAACATCAA CCTAATTGTT CAGAAAAGGC CTGAAATCTT GAAGCAGGAA
    ATAAAAGTTT TCTTTGTGAA GTACAATGAT CCCATCTATG TGAAACTAGA GAAGTTGGAC
    ATCATGATTC GTTTGGCATC CCAAGCCAAC ATTGCTCAGG TTCTGGCTGA ACTGAAAGAG
    TATGCCACAG AGGTGGATGT TGACTTTGTG CGCAAAGCTG TGCGAGCCAT TGGGCGGTGT
    GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CGTTGCTTGA TCTCATCCAA
    ACCAAAGTAA ATTATGTGGT CCAAGAGGCG ATTGTTGTCA TCAGGGACAT TTTCCGCAAA
    TACCCCAACA AATACGAAAG TATCATTGCC ACACTGTGTG AGAACTTAGA TTCTCTGGAT
    GAGCCAGACG CTCGAGCAGC TATGATTTGG ATTGTGGGAG AGTATGCTGA AAGAATTGAC
    AATGCTGATG AGTTACTAGA GAGCTTCCTG GAGGGTTTTC ATGATGAAAG CACCCAAGTG
    CAGCTCACTC TGCTCACTGC CATAGTGAAG CTGTTTCTCA AGAAACCGTC AGAAACACAG
    GAGCTGGTAC AGCAGGTCTT GAGTTTGGCA ACACAGGATT CTGATAATCC TGACCTTCGA
    GACCGGGGCT ATATTTATTG GCGCCTTCTC TCAACTGATC CTGTCACAGC TAAAGAAGTA
    GTCTTGTCTG AAAAGCCACT GATTTCTGAG GAGACAGATC TTATTGAACC AACTCTACTG
    GATGAGCTAA TCTGCCACAT TGGTTCTTTG GCCTCAGTGT ACCATAAGCC GCCCAATGCT
    TTTGTGGAAG GAAGTCATGG AATCCATCGC AAACACTTGC CAATACATCA TGGGAGCACT
    GATGCAGGTG ACAGCCCTGT TGGCGCCGCC ACTGCCACCA ACCTGGAGCA GCCTCAGGTT
    ATTCCCTCCC AGGGTGACCT TCTGGGGGAT CTTTTAAACC TTGACCTTGG TCCCCCAGTC
    AATGTGCCAC AGGTATCTTC CATGCAGATG GGAGCAGTGG ATCTCTTGGG AGGAGGCCTA
    GATAGTCTGC TTGGCAGTGA CCTTGGCGGG GGCATTGGAG GAAGTCCGGC AGTGGGACAG
    TCCTTCATCC CCTCATCAGT GCCTGCAACC TTTGCTCCCT CCCCTACTCC TGCTGTGGTC
    AGCAGTGGTC TGAATGACCT GTTTGAACTC TCCACGGGGA TAGGCATGGC ACCTGGTGGA
    TATGTGGCTC CTAAGGCTGT CTGGCTGCCT GCGGTAAAGG CTAAAGGCCT GGAGATTTCA
    GGAACATTTA CTCACCGCCA AGGGCACATC TATATGGAAA TGAACTTCAC CAACAAAGCT
    CTGCAGCACA TGACAGACTT CGCAATTCAG TTTAACAAGA ATAGCTTTGG TGTCATCCCT
    AGCACTCCTC TGGCCATCCA TACGCCACTG ATGCCAAACC AGAGCATTGA TGTCTCCCTG
    CCCCTCAACA CCTTGGGCCC TGTCATGAAG ATGGAGCCTC TGAACAACCT GCAGGTGGCT
    GTGAAAAACA ATATTGATGT CTTCTACTTC AGCTGCCTCA TCCCACTCAA CGTGCTTTTT
    GTAGAAGATG GCAAAATGGA GCGCCAGGTC TTCCTTGCAA CATGGAAGGA TATTCCCAAT
    GAAAATGAAC TTCAGTTTCA GATTAAGGAA TGTCATTTAA ATGCTGACAC TGTTTCCAGC
    AAGTTGCAAA ACAACAATGT TTATACTATT GCCAAGAGGA ATGTGGAAGG GCAGGACATG
    CTGTACCAAT CCCTGAAGCT CACTAATGGC ATTTGGATTT TGGCCGAGCT ACGTATCCAA
    CCAGGAAACC CCAATTACAC GCTGTCGCTG AAGTGTAGAG CTCCCGAGGT CTCCCAGTAC
    ATCTATCAGG TCTACGACAG CATTTTGAAA AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006056284.1
    CDS408..3263
    Translation

    Target ORF information:

    RefSeq Version XM_006056222.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis adaptor related protein complex 2 subunit beta 1 (AP2B1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006056222.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGACTGACT CCAAGTACTT CACAACCAAT AAAAAGGGAG AAATCTTTGA ATTAAAGGCT 
    GAACTCAACA ATGAAAAGAA AGAAAAGAGG AAGGAGGCTG TGAAGAAAGT GATTGCTGCT
    ATGACTGTGG GGAAAGACGT TAGCTCTCTT TTTCCAGATG TAGTGAACTG TATGCAGACG
    GATAATCTGG AACTGAAGAA GCTTGTGTAT CTCTACTTGA TGAACTATGC CAAGAGTCAG
    CCAGACATGG CCATCATGGC TGTCAACAGC TTTGTGAAGG ATTGTGAAGA TCCCAATCCT
    CTGATTCGAG CCTTGGCAGT CAGAACCATG GGGTGCATCC GGGTGGACAA GATTACAGAG
    TATCTCTGTG AGCCCCTCCG CAAGTGCTTA AAGGATGAAG ATCCCTACGT CCGGAAGACA
    GCAGCAGTCT GTGTGGCAAA ACTCCATGAC ATCAATGCCC AGATGGTGGA AGATCAGGGA
    TTTCTGGATT CTCTGCGGGA TCTCATAGCA GATTCAAATC CAATGGTGGT GGCTAATGCT
    GTAGCAGCAC TATCTGAAAT CAGTGAGTCT CACCCCAACA GCAACTTACT CGATCTGAAT
    CCACAGAACA TTAATAAGCT ACTGACAGCC CTGAATGAGT GCACCGAATG GGGCCAGATT
    TTCATCCTGG ACTGCTTATC TAATTACAAT CCTAAAGATG ACCGGGAGGC TCAGAGCATC
    TGTGAGCGGG TAACTCCCCG GTTATCTCAT GCCAACTCAG CAGTGGTGCT TTCAGCAGTA
    AAAGTCCTAA TGAAATTTTT AGAGTTGCTG CCCAAGGACT CTGACTACTA CAGTATGCTG
    CTGAAGAAGT TGGCCCCTCC ACTTGTCACT CTGCTGTCTG GGGAGCCAGA GGTGCAATAC
    GTCGCCCTGA GGAACATCAA CCTAATTGTT CAGAAAAGGC CTGAAATCTT GAAGCAGGAA
    ATAAAAGTTT TCTTTGTGAA GTACAATGAT CCCATCTATG TGAAACTAGA GAAGTTGGAC
    ATCATGATTC GTTTGGCATC CCAAGCCAAC ATTGCTCAGG TTCTGGCTGA ACTGAAAGAG
    TATGCCACAG AGGTGGATGT TGACTTTGTG CGCAAAGCTG TGCGAGCCAT TGGGCGGTGT
    GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CGTTGCTTGA TCTCATCCAA
    ACCAAAGTAA ATTATGTGGT CCAAGAGGCG ATTGTTGTCA TCAGGGACAT TTTCCGCAAA
    TACCCCAACA AATACGAAAG TATCATTGCC ACACTGTGTG AGAACTTAGA TTCTCTGGAT
    GAGCCAGACG CTCGAGCAGC TATGATTTGG ATTGTGGGAG AGTATGCTGA AAGAATTGAC
    AATGCTGATG AGTTACTAGA GAGCTTCCTG GAGGGTTTTC ATGATGAAAG CACCCAAGTG
    CAGCTCACTC TGCTCACTGC CATAGTGAAG CTGTTTCTCA AGAAACCGTC AGAAACACAG
    GAGCTGGTAC AGCAGGTCTT GAGTTTGGCA ACACAGGATT CTGATAATCC TGACCTTCGA
    GACCGGGGCT ATATTTATTG GCGCCTTCTC TCAACTGATC CTGTCACAGC TAAAGAAGTA
    GTCTTGTCTG AAAAGCCACT GATTTCTGAG GAGACAGATC TTATTGAACC AACTCTACTG
    GATGAGCTAA TCTGCCACAT TGGTTCTTTG GCCTCAGTGT ACCATAAGCC GCCCAATGCT
    TTTGTGGAAG GAAGTCATGG AATCCATCGC AAACACTTGC CAATACATCA TGGGAGCACT
    GATGCAGGTG ACAGCCCTGT TGGCGCCGCC ACTGCCACCA ACCTGGAGCA GCCTCAGGTT
    ATTCCCTCCC AGGGTGACCT TCTGGGGGAT CTTTTAAACC TTGACCTTGG TCCCCCAGTC
    AATGTGCCAC AGGTATCTTC CATGCAGATG GGAGCAGTGG ATCTCTTGGG AGGAGGCCTA
    GATAGTCTGC TTGGCAGTGA CCTTGGCGGG GGCATTGGAG GAAGTCCGGC AGTGGGACAG
    TCCTTCATCC CCTCATCAGT GCCTGCAACC TTTGCTCCCT CCCCTACTCC TGCTGTGGTC
    AGCAGTGGTC TGAATGACCT GTTTGAACTC TCCACGGGGA TAGGCATGGC ACCTGGTGGA
    TATGTGGCTC CTAAGGCTGT CTGGCTGCCT GCGGTAAAGG CTAAAGGCCT GGAGATTTCA
    GGAACATTTA CTCACCGCCA AGGGCACATC TATATGGAAA TGAACTTCAC CAACAAAGCT
    CTGCAGCACA TGACAGACTT CGCAATTCAG TTTAACAAGA ATAGCTTTGG TGTCATCCCT
    AGCACTCCTC TGGCCATCCA TACGCCACTG ATGCCAAACC AGAGCATTGA TGTCTCCCTG
    CCCCTCAACA CCTTGGGCCC TGTCATGAAG ATGGAGCCTC TGAACAACCT GCAGGTGGCT
    GTGAAAAACA ATATTGATGT CTTCTACTTC AGCTGCCTCA TCCCACTCAA CGTGCTTTTT
    GTAGAAGATG GCAAAATGGA GCGCCAGGTC TTCCTTGCAA CATGGAAGGA TATTCCCAAT
    GAAAATGAAC TTCAGTTTCA GATTAAGGAA TGTCATTTAA ATGCTGACAC TGTTTCCAGC
    AAGTTGCAAA ACAACAATGT TTATACTATT GCCAAGAGGA ATGTGGAAGG GCAGGACATG
    CTGTACCAAT CCCTGAAGCT CACTAATGGC ATTTGGATTT TGGCCGAGCT ACGTATCCAA
    CCAGGAAACC CCAATTACAC GCTGTCGCTG AAGTGTAGAG CTCCCGAGGT CTCCCAGTAC
    ATCTATCAGG TCTACGACAG CATTTTGAAA AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu13376
    Clone ID Related Accession (Same CDS sequence) XM_006056223.2 , XM_006056223.1
    Accession Version XM_006056223.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2814bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product AP-2 complex subunit beta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006056223.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACTGACT CCAAGTACTT CACAACCAAT AAAAAGGGAG AAATCTTTGA ATTAAAGGCT 
    GAACTCAACA ATGAAAAGAA AGAAAAGAGG AAGGAGGCTG TGAAGAAAGT GATTGCTGCT
    ATGACTGTGG GGAAAGACGT TAGCTCTCTT TTTCCAGATG TAGTGAACTG TATGCAGACG
    GATAATCTGG AACTGAAGAA GCTTGTGTAT CTCTACTTGA TGAACTATGC CAAGAGTCAG
    CCAGACATGG CCATCATGGC TGTCAACAGC TTTGTGAAGG ATTGTGAAGA TCCCAATCCT
    CTGATTCGAG CCTTGGCAGT CAGAACCATG GGGTGCATCC GGGTGGACAA GATTACAGAG
    TATCTCTGTG AGCCCCTCCG CAAGTGCTTA AAGGATGAAG ATCCCTACGT CCGGAAGACA
    GCAGCAGTCT GTGTGGCAAA ACTCCATGAC ATCAATGCCC AGATGGTGGA AGATCAGGGA
    TTTCTGGATT CTCTGCGGGA TCTCATAGCA GATTCAAATC CAATGGTGGT GGCTAATGCT
    GTAGCAGCAC TATCTGAAAT CAGTGAGTCT CACCCCAACA GCAACTTACT CGATCTGAAT
    CCACAGAACA TTAATAAGCT ACTGACAGCC CTGAATGAGT GCACCGAATG GGGCCAGATT
    TTCATCCTGG ACTGCTTATC TAATTACAAT CCTAAAGATG ACCGGGAGGC TCAGAGCATC
    TGTGAGCGGG TAACTCCCCG GTTATCTCAT GCCAACTCAG CAGTGGTGCT TTCAGCAGTA
    AAAGTCCTAA TGAAATTTTT AGAGTTGCTG CCCAAGGACT CTGACTACTA CAGTATGCTG
    CTGAAGAAGT TGGCCCCTCC ACTTGTCACT CTGCTGTCTG GGGAGCCAGA GGTGCAATAC
    GTCGCCCTGA GGAACATCAA CCTAATTGTT CAGAAAAGGC CTGAAATCTT GAAGCAGGAA
    ATAAAAGTTT TCTTTGTGAA GTACAATGAT CCCATCTATG TGAAACTAGA GAAGTTGGAC
    ATCATGATTC GTTTGGCATC CCAAGCCAAC ATTGCTCAGG TTCTGGCTGA ACTGAAAGAG
    TATGCCACAG AGGTGGATGT TGACTTTGTG CGCAAAGCTG TGCGAGCCAT TGGGCGGTGT
    GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CGTTGCTTGA TCTCATCCAA
    ACCAAAGTAA ATTATGTGGT CCAAGAGGCG ATTGTTGTCA TCAGGGACAT TTTCCGCAAA
    TACCCCAACA AATACGAAAG TATCATTGCC ACACTGTGTG AGAACTTAGA TTCTCTGGAT
    GAGCCAGACG CTCGAGCAGC TATGATTTGG ATTGTGGGAG AGTATGCTGA AAGAATTGAC
    AATGCTGATG AGTTACTAGA GAGCTTCCTG GAGGGTTTTC ATGATGAAAG CACCCAAGTG
    CAGCTCACTC TGCTCACTGC CATAGTGAAG CTGTTTCTCA AGAAACCGTC AGAAACACAG
    GAGCTGGTAC AGCAGGTCTT GAGTTTGGCA ACACAGGATT CTGATAATCC TGACCTTCGA
    GACCGGGGCT ATATTTATTG GCGCCTTCTC TCAACTGATC CTGTCACAGC TAAAGAAGTA
    GTCTTGTCTG AAAAGCCACT GATTTCTGAG GAGACAGATC TTATTGAACC AACTCTACTG
    GATGAGCTAA TCTGCCACAT TGGTTCTTTG GCCTCAGTGT ACCATAAGCC GCCCAATGCT
    TTTGTGGAAG GAAGTCATGG AATCCATCGC AAACACTTGC CAATACATCA TGGGAGCACT
    GATGCAGGTG ACAGCCCTGT TGGCGCCGCC ACTGCCACCA ACCTGGAGCA GCCTCAGGTT
    ATTCCCTCCC AGGGTGACCT TCTGGGGGAT CTTTTAAACC TTGACCTTGG TCCCCCAGTC
    AATGTGCCAC AGGTATCTTC CATGCAGATG GGAGCAGTGG ATCTCTTGGG AGGAGGCCTA
    GATAGTCTGG TGGGACAGTC CTTCATCCCC TCATCAGTGC CTGCAACCTT TGCTCCCTCC
    CCTACTCCTG CTGTGGTCAG CAGTGGTCTG AATGACCTGT TTGAACTCTC CACGGGGATA
    GGCATGGCAC CTGGTGGATA TGTGGCTCCT AAGGCTGTCT GGCTGCCTGC GGTAAAGGCT
    AAAGGCCTGG AGATTTCAGG AACATTTACT CACCGCCAAG GGCACATCTA TATGGAAATG
    AACTTCACCA ACAAAGCTCT GCAGCACATG ACAGACTTCG CAATTCAGTT TAACAAGAAT
    AGCTTTGGTG TCATCCCTAG CACTCCTCTG GCCATCCATA CGCCACTGAT GCCAAACCAG
    AGCATTGATG TCTCCCTGCC CCTCAACACC TTGGGCCCTG TCATGAAGAT GGAGCCTCTG
    AACAACCTGC AGGTGGCTGT GAAAAACAAT ATTGATGTCT TCTACTTCAG CTGCCTCATC
    CCACTCAACG TGCTTTTTGT AGAAGATGGC AAAATGGAGC GCCAGGTCTT CCTTGCAACA
    TGGAAGGATA TTCCCAATGA AAATGAACTT CAGTTTCAGA TTAAGGAATG TCATTTAAAT
    GCTGACACTG TTTCCAGCAA GTTGCAAAAC AACAATGTTT ATACTATTGC CAAGAGGAAT
    GTGGAAGGGC AGGACATGCT GTACCAATCC CTGAAGCTCA CTAATGGCAT TTGGATTTTG
    GCCGAGCTAC GTATCCAACC AGGAAACCCC AATTACACGC TGTCGCTGAA GTGTAGAGCT
    CCCGAGGTCT CCCAGTACAT CTATCAGGTC TACGACAGCA TTTTGAAAAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006056285.1
    CDS175..2988
    Translation

    Target ORF information:

    RefSeq Version XM_006056223.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis adaptor related protein complex 2 subunit beta 1 (AP2B1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006056223.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACTGACT CCAAGTACTT CACAACCAAT AAAAAGGGAG AAATCTTTGA ATTAAAGGCT 
    GAACTCAACA ATGAAAAGAA AGAAAAGAGG AAGGAGGCTG TGAAGAAAGT GATTGCTGCT
    ATGACTGTGG GGAAAGACGT TAGCTCTCTT TTTCCAGATG TAGTGAACTG TATGCAGACG
    GATAATCTGG AACTGAAGAA GCTTGTGTAT CTCTACTTGA TGAACTATGC CAAGAGTCAG
    CCAGACATGG CCATCATGGC TGTCAACAGC TTTGTGAAGG ATTGTGAAGA TCCCAATCCT
    CTGATTCGAG CCTTGGCAGT CAGAACCATG GGGTGCATCC GGGTGGACAA GATTACAGAG
    TATCTCTGTG AGCCCCTCCG CAAGTGCTTA AAGGATGAAG ATCCCTACGT CCGGAAGACA
    GCAGCAGTCT GTGTGGCAAA ACTCCATGAC ATCAATGCCC AGATGGTGGA AGATCAGGGA
    TTTCTGGATT CTCTGCGGGA TCTCATAGCA GATTCAAATC CAATGGTGGT GGCTAATGCT
    GTAGCAGCAC TATCTGAAAT CAGTGAGTCT CACCCCAACA GCAACTTACT CGATCTGAAT
    CCACAGAACA TTAATAAGCT ACTGACAGCC CTGAATGAGT GCACCGAATG GGGCCAGATT
    TTCATCCTGG ACTGCTTATC TAATTACAAT CCTAAAGATG ACCGGGAGGC TCAGAGCATC
    TGTGAGCGGG TAACTCCCCG GTTATCTCAT GCCAACTCAG CAGTGGTGCT TTCAGCAGTA
    AAAGTCCTAA TGAAATTTTT AGAGTTGCTG CCCAAGGACT CTGACTACTA CAGTATGCTG
    CTGAAGAAGT TGGCCCCTCC ACTTGTCACT CTGCTGTCTG GGGAGCCAGA GGTGCAATAC
    GTCGCCCTGA GGAACATCAA CCTAATTGTT CAGAAAAGGC CTGAAATCTT GAAGCAGGAA
    ATAAAAGTTT TCTTTGTGAA GTACAATGAT CCCATCTATG TGAAACTAGA GAAGTTGGAC
    ATCATGATTC GTTTGGCATC CCAAGCCAAC ATTGCTCAGG TTCTGGCTGA ACTGAAAGAG
    TATGCCACAG AGGTGGATGT TGACTTTGTG CGCAAAGCTG TGCGAGCCAT TGGGCGGTGT
    GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CGTTGCTTGA TCTCATCCAA
    ACCAAAGTAA ATTATGTGGT CCAAGAGGCG ATTGTTGTCA TCAGGGACAT TTTCCGCAAA
    TACCCCAACA AATACGAAAG TATCATTGCC ACACTGTGTG AGAACTTAGA TTCTCTGGAT
    GAGCCAGACG CTCGAGCAGC TATGATTTGG ATTGTGGGAG AGTATGCTGA AAGAATTGAC
    AATGCTGATG AGTTACTAGA GAGCTTCCTG GAGGGTTTTC ATGATGAAAG CACCCAAGTG
    CAGCTCACTC TGCTCACTGC CATAGTGAAG CTGTTTCTCA AGAAACCGTC AGAAACACAG
    GAGCTGGTAC AGCAGGTCTT GAGTTTGGCA ACACAGGATT CTGATAATCC TGACCTTCGA
    GACCGGGGCT ATATTTATTG GCGCCTTCTC TCAACTGATC CTGTCACAGC TAAAGAAGTA
    GTCTTGTCTG AAAAGCCACT GATTTCTGAG GAGACAGATC TTATTGAACC AACTCTACTG
    GATGAGCTAA TCTGCCACAT TGGTTCTTTG GCCTCAGTGT ACCATAAGCC GCCCAATGCT
    TTTGTGGAAG GAAGTCATGG AATCCATCGC AAACACTTGC CAATACATCA TGGGAGCACT
    GATGCAGGTG ACAGCCCTGT TGGCGCCGCC ACTGCCACCA ACCTGGAGCA GCCTCAGGTT
    ATTCCCTCCC AGGGTGACCT TCTGGGGGAT CTTTTAAACC TTGACCTTGG TCCCCCAGTC
    AATGTGCCAC AGGTATCTTC CATGCAGATG GGAGCAGTGG ATCTCTTGGG AGGAGGCCTA
    GATAGTCTGG TGGGACAGTC CTTCATCCCC TCATCAGTGC CTGCAACCTT TGCTCCCTCC
    CCTACTCCTG CTGTGGTCAG CAGTGGTCTG AATGACCTGT TTGAACTCTC CACGGGGATA
    GGCATGGCAC CTGGTGGATA TGTGGCTCCT AAGGCTGTCT GGCTGCCTGC GGTAAAGGCT
    AAAGGCCTGG AGATTTCAGG AACATTTACT CACCGCCAAG GGCACATCTA TATGGAAATG
    AACTTCACCA ACAAAGCTCT GCAGCACATG ACAGACTTCG CAATTCAGTT TAACAAGAAT
    AGCTTTGGTG TCATCCCTAG CACTCCTCTG GCCATCCATA CGCCACTGAT GCCAAACCAG
    AGCATTGATG TCTCCCTGCC CCTCAACACC TTGGGCCCTG TCATGAAGAT GGAGCCTCTG
    AACAACCTGC AGGTGGCTGT GAAAAACAAT ATTGATGTCT TCTACTTCAG CTGCCTCATC
    CCACTCAACG TGCTTTTTGT AGAAGATGGC AAAATGGAGC GCCAGGTCTT CCTTGCAACA
    TGGAAGGATA TTCCCAATGA AAATGAACTT CAGTTTCAGA TTAAGGAATG TCATTTAAAT
    GCTGACACTG TTTCCAGCAA GTTGCAAAAC AACAATGTTT ATACTATTGC CAAGAGGAAT
    GTGGAAGGGC AGGACATGCT GTACCAATCC CTGAAGCTCA CTAATGGCAT TTGGATTTTG
    GCCGAGCTAC GTATCCAACC AGGAAACCCC AATTACACGC TGTCGCTGAA GTGTAGAGCT
    CCCGAGGTCT CCCAGTACAT CTATCAGGTC TACGACAGCA TTTTGAAAAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu13376
    Clone ID Related Accession (Same CDS sequence) XM_006056223.2 , XM_006056223.1
    Accession Version XM_006056223.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2814bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product AP-1 complex subunit beta-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784736.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACTGACT CCAAGTACTT CACAACCAAT AAAAAGGGAG AAATCTTTGA ATTAAAGGCT 
    GAACTCAACA ATGAAAAGAA AGAAAAGAGG AAGGAGGCTG TGAAGAAAGT GATTGCTGCT
    ATGACTGTGG GGAAAGACGT TAGCTCTCTT TTTCCAGATG TAGTGAACTG TATGCAGACG
    GATAATCTGG AACTGAAGAA GCTTGTGTAT CTCTACTTGA TGAACTATGC CAAGAGTCAG
    CCAGACATGG CCATCATGGC TGTCAACAGC TTTGTGAAGG ATTGTGAAGA TCCCAATCCT
    CTGATTCGAG CCTTGGCAGT CAGAACCATG GGGTGCATCC GGGTGGACAA GATTACAGAG
    TATCTCTGTG AGCCCCTCCG CAAGTGCTTA AAGGATGAAG ATCCCTACGT CCGGAAGACA
    GCAGCAGTCT GTGTGGCAAA ACTCCATGAC ATCAATGCCC AGATGGTGGA AGATCAGGGA
    TTTCTGGATT CTCTGCGGGA TCTCATAGCA GATTCAAATC CAATGGTGGT GGCTAATGCT
    GTAGCAGCAC TATCTGAAAT CAGTGAGTCT CACCCCAACA GCAACTTACT CGATCTGAAT
    CCACAGAACA TTAATAAGCT ACTGACAGCC CTGAATGAGT GCACCGAATG GGGCCAGATT
    TTCATCCTGG ACTGCTTATC TAATTACAAT CCTAAAGATG ACCGGGAGGC TCAGAGCATC
    TGTGAGCGGG TAACTCCCCG GTTATCTCAT GCCAACTCAG CAGTGGTGCT TTCAGCAGTA
    AAAGTCCTAA TGAAATTTTT AGAGTTGCTG CCCAAGGACT CTGACTACTA CAGTATGCTG
    CTGAAGAAGT TGGCCCCTCC ACTTGTCACT CTGCTGTCTG GGGAGCCAGA GGTGCAATAC
    GTCGCCCTGA GGAACATCAA CCTAATTGTT CAGAAAAGGC CTGAAATCTT GAAGCAGGAA
    ATAAAAGTTT TCTTTGTGAA GTACAATGAT CCCATCTATG TGAAACTAGA GAAGTTGGAC
    ATCATGATTC GTTTGGCATC CCAAGCCAAC ATTGCTCAGG TTCTGGCTGA ACTGAAAGAG
    TATGCCACAG AGGTGGATGT TGACTTTGTG CGCAAAGCTG TGCGAGCCAT TGGGCGGTGT
    GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CGTTGCTTGA TCTCATCCAA
    ACCAAAGTAA ATTATGTGGT CCAAGAGGCG ATTGTTGTCA TCAGGGACAT TTTCCGCAAA
    TACCCCAACA AATACGAAAG TATCATTGCC ACACTGTGTG AGAACTTAGA TTCTCTGGAT
    GAGCCAGACG CTCGAGCAGC TATGATTTGG ATTGTGGGAG AGTATGCTGA AAGAATTGAC
    AATGCTGATG AGTTACTAGA GAGCTTCCTG GAGGGTTTTC ATGATGAAAG CACCCAAGTG
    CAGCTCACTC TGCTCACTGC CATAGTGAAG CTGTTTCTCA AGAAACCGTC AGAAACACAG
    GAGCTGGTAC AGCAGGTCTT GAGTTTGGCA ACACAGGATT CTGATAATCC TGACCTTCGA
    GACCGGGGCT ATATTTATTG GCGCCTTCTC TCAACTGATC CTGTCACAGC TAAAGAAGTA
    GTCTTGTCTG AAAAGCCACT GATTTCTGAG GAGACAGATC TTATTGAACC AACTCTACTG
    GATGAGCTAA TCTGCCACAT TGGTTCTTTG GCCTCAGTGT ACCATAAGCC GCCCAATGCT
    TTTGTGGAAG GAAGTCATGG AATCCATCGC AAACACTTGC CAATACATCA TGGGAGCACT
    GATGCAGGTG ACAGCCCTGT TGGCGCCGCC ACTGCCACCA ACCTGGAGCA GCCTCAGGTT
    ATTCCCTCCC AGGGTGACCT TCTGGGGGAT CTTTTAAACC TTGACCTTGG TCCCCCAGTC
    AATGTGCCAC AGGTATCTTC CATGCAGATG GGAGCAGTGG ATCTCTTGGG AGGAGGCCTA
    GATAGTCTGG TGGGACAGTC CTTCATCCCC TCATCAGTGC CTGCAACCTT TGCTCCCTCC
    CCTACTCCTG CTGTGGTCAG CAGTGGTCTG AATGACCTGT TTGAACTCTC CACGGGGATA
    GGCATGGCAC CTGGTGGATA TGTGGCTCCT AAGGCTGTCT GGCTGCCTGC GGTAAAGGCT
    AAAGGCCTGG AGATTTCAGG AACATTTACT CACCGCCAAG GGCACATCTA TATGGAAATG
    AACTTCACCA ACAAAGCTCT GCAGCACATG ACAGACTTCG CAATTCAGTT TAACAAGAAT
    AGCTTTGGTG TCATCCCTAG CACTCCTCTG GCCATCCATA CGCCACTGAT GCCAAACCAG
    AGCATTGATG TCTCCCTGCC CCTCAACACC TTGGGCCCTG TCATGAAGAT GGAGCCTCTG
    AACAACCTGC AGGTGGCTGT GAAAAACAAT ATTGATGTCT TCTACTTCAG CTGCCTCATC
    CCACTCAACG TGCTTTTTGT AGAAGATGGC AAAATGGAGC GCCAGGTCTT CCTTGCAACA
    TGGAAGGATA TTCCCAATGA AAATGAACTT CAGTTTCAGA TTAAGGAATG TCATTTAAAT
    GCTGACACTG TTTCCAGCAA GTTGCAAAAC AACAATGTTT ATACTATTGC CAAGAGGAAT
    GTGGAAGGGC AGGACATGCT GTACCAATCC CTGAAGCTCA CTAATGGCAT TTGGATTTTG
    GCCGAGCTAC GTATCCAACC AGGAAACCCC AATTACACGC TGTCGCTGAA GTGTAGAGCT
    CCCGAGGTCT CCCAGTACAT CTATCAGGTC TACGACAGCA TTTTGAAAAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006056285.1
    CDS152..2965
    Translation

    Target ORF information:

    RefSeq Version XM_006056223.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis adaptor-related protein complex 2, beta 1 subunit (AP2B1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006056223.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACTGACT CCAAGTACTT CACAACCAAT AAAAAGGGAG AAATCTTTGA ATTAAAGGCT 
    GAACTCAACA ATGAAAAGAA AGAAAAGAGG AAGGAGGCTG TGAAGAAAGT GATTGCTGCT
    ATGACTGTGG GGAAAGACGT TAGCTCTCTT TTTCCAGATG TAGTGAACTG TATGCAGACG
    GATAATCTGG AACTGAAGAA GCTTGTGTAT CTCTACTTGA TGAACTATGC CAAGAGTCAG
    CCAGACATGG CCATCATGGC TGTCAACAGC TTTGTGAAGG ATTGTGAAGA TCCCAATCCT
    CTGATTCGAG CCTTGGCAGT CAGAACCATG GGGTGCATCC GGGTGGACAA GATTACAGAG
    TATCTCTGTG AGCCCCTCCG CAAGTGCTTA AAGGATGAAG ATCCCTACGT CCGGAAGACA
    GCAGCAGTCT GTGTGGCAAA ACTCCATGAC ATCAATGCCC AGATGGTGGA AGATCAGGGA
    TTTCTGGATT CTCTGCGGGA TCTCATAGCA GATTCAAATC CAATGGTGGT GGCTAATGCT
    GTAGCAGCAC TATCTGAAAT CAGTGAGTCT CACCCCAACA GCAACTTACT CGATCTGAAT
    CCACAGAACA TTAATAAGCT ACTGACAGCC CTGAATGAGT GCACCGAATG GGGCCAGATT
    TTCATCCTGG ACTGCTTATC TAATTACAAT CCTAAAGATG ACCGGGAGGC TCAGAGCATC
    TGTGAGCGGG TAACTCCCCG GTTATCTCAT GCCAACTCAG CAGTGGTGCT TTCAGCAGTA
    AAAGTCCTAA TGAAATTTTT AGAGTTGCTG CCCAAGGACT CTGACTACTA CAGTATGCTG
    CTGAAGAAGT TGGCCCCTCC ACTTGTCACT CTGCTGTCTG GGGAGCCAGA GGTGCAATAC
    GTCGCCCTGA GGAACATCAA CCTAATTGTT CAGAAAAGGC CTGAAATCTT GAAGCAGGAA
    ATAAAAGTTT TCTTTGTGAA GTACAATGAT CCCATCTATG TGAAACTAGA GAAGTTGGAC
    ATCATGATTC GTTTGGCATC CCAAGCCAAC ATTGCTCAGG TTCTGGCTGA ACTGAAAGAG
    TATGCCACAG AGGTGGATGT TGACTTTGTG CGCAAAGCTG TGCGAGCCAT TGGGCGGTGT
    GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CGTTGCTTGA TCTCATCCAA
    ACCAAAGTAA ATTATGTGGT CCAAGAGGCG ATTGTTGTCA TCAGGGACAT TTTCCGCAAA
    TACCCCAACA AATACGAAAG TATCATTGCC ACACTGTGTG AGAACTTAGA TTCTCTGGAT
    GAGCCAGACG CTCGAGCAGC TATGATTTGG ATTGTGGGAG AGTATGCTGA AAGAATTGAC
    AATGCTGATG AGTTACTAGA GAGCTTCCTG GAGGGTTTTC ATGATGAAAG CACCCAAGTG
    CAGCTCACTC TGCTCACTGC CATAGTGAAG CTGTTTCTCA AGAAACCGTC AGAAACACAG
    GAGCTGGTAC AGCAGGTCTT GAGTTTGGCA ACACAGGATT CTGATAATCC TGACCTTCGA
    GACCGGGGCT ATATTTATTG GCGCCTTCTC TCAACTGATC CTGTCACAGC TAAAGAAGTA
    GTCTTGTCTG AAAAGCCACT GATTTCTGAG GAGACAGATC TTATTGAACC AACTCTACTG
    GATGAGCTAA TCTGCCACAT TGGTTCTTTG GCCTCAGTGT ACCATAAGCC GCCCAATGCT
    TTTGTGGAAG GAAGTCATGG AATCCATCGC AAACACTTGC CAATACATCA TGGGAGCACT
    GATGCAGGTG ACAGCCCTGT TGGCGCCGCC ACTGCCACCA ACCTGGAGCA GCCTCAGGTT
    ATTCCCTCCC AGGGTGACCT TCTGGGGGAT CTTTTAAACC TTGACCTTGG TCCCCCAGTC
    AATGTGCCAC AGGTATCTTC CATGCAGATG GGAGCAGTGG ATCTCTTGGG AGGAGGCCTA
    GATAGTCTGG TGGGACAGTC CTTCATCCCC TCATCAGTGC CTGCAACCTT TGCTCCCTCC
    CCTACTCCTG CTGTGGTCAG CAGTGGTCTG AATGACCTGT TTGAACTCTC CACGGGGATA
    GGCATGGCAC CTGGTGGATA TGTGGCTCCT AAGGCTGTCT GGCTGCCTGC GGTAAAGGCT
    AAAGGCCTGG AGATTTCAGG AACATTTACT CACCGCCAAG GGCACATCTA TATGGAAATG
    AACTTCACCA ACAAAGCTCT GCAGCACATG ACAGACTTCG CAATTCAGTT TAACAAGAAT
    AGCTTTGGTG TCATCCCTAG CACTCCTCTG GCCATCCATA CGCCACTGAT GCCAAACCAG
    AGCATTGATG TCTCCCTGCC CCTCAACACC TTGGGCCCTG TCATGAAGAT GGAGCCTCTG
    AACAACCTGC AGGTGGCTGT GAAAAACAAT ATTGATGTCT TCTACTTCAG CTGCCTCATC
    CCACTCAACG TGCTTTTTGT AGAAGATGGC AAAATGGAGC GCCAGGTCTT CCTTGCAACA
    TGGAAGGATA TTCCCAATGA AAATGAACTT CAGTTTCAGA TTAAGGAATG TCATTTAAAT
    GCTGACACTG TTTCCAGCAA GTTGCAAAAC AACAATGTTT ATACTATTGC CAAGAGGAAT
    GTGGAAGGGC AGGACATGCT GTACCAATCC CTGAAGCTCA CTAATGGCAT TTGGATTTTG
    GCCGAGCTAC GTATCCAACC AGGAAACCCC AATTACACGC TGTCGCTGAA GTGTAGAGCT
    CCCGAGGTCT CCCAGTACAT CTATCAGGTC TACGACAGCA TTTTGAAAAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu62377
    Clone ID Related Accession (Same CDS sequence) XM_025281093.1
    Accession Version XM_025281093.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2643bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product AP-2 complex subunit beta isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGACTGACT CCAAGTACTT CACAACCAAT AAAAAGGGAG AAATCTTTGA ATTAAAGGCT 
    GAACTCAACA ATGAAAAGAA AGAAAAGAGG AAGGAGGCTG TGAAGAAAGT GATTGCTGCT
    ATGACTGTGG GGAAAGACGT TAGCTCTCTT TTTCCAGATG TAGTGAACTG TATGCAGACG
    GATAATCTGG AACTGAAGAA GCTTGTGTAT CTCTACTTGA TGAACTATGC CAAGAGTCAG
    CCAGACATGG CCATCATGGC TGTCAACAGC TTTGTGAAGG ATTGTGAAGA TCCCAATCCT
    CTGATTCGAG CCTTGGCAGT CAGAACCATG GGGTGCATCC GGGTGGACAA GATTACAGAG
    TATCTCTGTG AGCCCCTCCG CAAGTGCTTA AAGGATGAAG ATCCCTACGT CCGGAAGACA
    GCAGCAGTCT GTGTGGCAAA ACTCCATGAC ATCAATGCCC AGATGGTGGA AGATCAGGGA
    TTTCTGGATT CTCTGCGGGA TCTCATAGCA GATTCAAATC CAATGGTGGT GGCTAATGCT
    GTAGCAGCAC TATCTGAAAT CAGTGAGTCT CACCCCAACA GCAACTTACT CGATCTGAAT
    CCACAGAACA TTAATAAGCT ACTGACAGCC CTGAATGAGT GCACCGAATG GGGCCAGATT
    TTCATCCTGG ACTGCTTATC TAATTACAAT CCTAAAGATG ACCGGGAGGC TCAGAGCATC
    TGTGAGCGGG TAACTCCCCG GTTATCTCAT GCCAACTCAG CAGTGGTGCT TTCAGCAGTA
    AAAGTCCTAA TGAAATTTTT AGAGTTGCTG CCCAAGGACT CTGACTACTA CAGTATGCTG
    CTGAAGAAGT TGGCCCCTCC ACTTGTCACT CTGCTGTCTG GGGAGCCAGA GGTGCAATAC
    GTCGCCCTGA GGAACATCAA CCTAATTGTT CAGAAAAGGC CTGAAATCTT GAAGCAGGAA
    ATAAAAGTTT TCTTTGTGAA GTACAATGAT CCCATCTATG TGAAACTAGA GAAGTTGGAC
    ATCATGATTC GTTTGGCATC CCAAGCCAAC ATTGCTCAGG TTCTGGCTGA ACTGAAAGAG
    TATGCCACAG AGGTGGATGT TGACTTTGTG CGCAAAGCTG TGCGAGCCAT TGGGCGGTGT
    GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CGTTGCTTGA TCTCATCCAA
    ACCAAAGTAA ATTATGTGGT CCAAGAGGCG ATTGTTGTCA TCAGGGACAT TTTCCGCAAA
    TACCCCAACA AATACGAAAG TATCATTGCC ACACTGTGTG AGAACTTAGA TTCTCTGGAT
    GAGCCAGACG CTCGAGCAGC TATGATTTGG ATTGTGGGAG AGTATGCTGA AAGAATTGAC
    AATGCTGATG AGTTACTAGA GAGCTTCCTG GAGGGTTTTC ATGATGAAAG CACCCAAGTG
    CAGCTCACTC TGCTCACTGC CATAGTGAAG CTGTTTCTCA AGAAACCGTC AGAAACACAG
    GAGCTGGTAC AGCAGGTCTT GAGTTTGGCA ACACAGGATT CTGATAATCC TGACCTTCGA
    GACCGGGGCT ATATTTATTG GCGCCTTCTC TCAACTGATC CTGTCACAGC TAAAGAAGTA
    GTCTTGTCTG AAAAGCCACT GATTTCTGAG GAGACAGATC TTATTGAACC AACTCTACTG
    GATGAGCTAA TCTGCCACAT TGGTTCTTTG GCCTCAGTGT ACCATAAGCC GCCCAATGCT
    TTTGTGGAAG GAAGTCATGG AATCCATCGC AAACACTTGC CAATACATCA TGGGAGCACT
    GATGCAGGTG ACAGCCCTGT TGGCGCCGCC ACTGCCACCA ACCTGGAGCA GCCTCAGGTT
    ATTCCCTCCC AGGGTGACCT TCTGGGGGAT CTTTTAAACC TTGACCTTGG TCCCCCAGTC
    AATGTGCCAC AGGTATCTTC CATGCAGATG GGAGCAGTGG ATCTCTTGGG AGGAGGCCTA
    GATAGTCTGC TTGGCAGTGA CCTTGGCGGG GGCATTGGAG GAAGTCCGGC AGTGGGACAG
    TCCTTCATCC CCTCATCAGT GCCTGCAACC TTTGCTCCCT CCCCTACTCC TGCTGTGGTC
    AGCAGTGGTC TGAATGACCT GTTTGAACTC TCCACGGGGA TAGGCATGGC ACCTGGTGGA
    TATGTGGCTC CTAAGGCTGT CTGGCTGCCT GCGGTAAAGG CTAAAGGCCT GGAGATTTCA
    GGAACATTTA CTCACCGCCA AGGGCACATC TATATGGAAA TGAACTTCAC CAACAAAGCT
    CTGCAGCACA TGACAGACTT CGCAATTCAG TTTAACAAGA ATAGCTTTGG TGTCATCCCT
    AGCACTCCTC TGGCCATCCA TACGCCACTG ATGCCAAACC AGAGCATTGA TGTCTCCCTG
    CCCCTCAACA CCTTGGGCCC TGTCATGAAG ATGGAGCCTC TGAACAACCT GCAGGTGGCT
    GTGAAAAACA ATATTGATGT CTTCTACTTC AGCTGCCTCA TCCCACTCAA CGTGCTTTTT
    GTAGAAGATG GCAAAATGGA GCGCCAGGTC TTCCTTGCAA CATGGAAGGA TATTCCCAAT
    GAAAATGAAC TTCAGTTTCA GATTAAGGAA TGTCATTTAA ATGCTGCTGT CGCTGAAGTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025136878.1
    CDS175..2817
    Translation

    Target ORF information:

    RefSeq Version XM_025281093.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis adaptor related protein complex 2 subunit beta 1 (AP2B1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025281093.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGACTGACT CCAAGTACTT CACAACCAAT AAAAAGGGAG AAATCTTTGA ATTAAAGGCT 
    GAACTCAACA ATGAAAAGAA AGAAAAGAGG AAGGAGGCTG TGAAGAAAGT GATTGCTGCT
    ATGACTGTGG GGAAAGACGT TAGCTCTCTT TTTCCAGATG TAGTGAACTG TATGCAGACG
    GATAATCTGG AACTGAAGAA GCTTGTGTAT CTCTACTTGA TGAACTATGC CAAGAGTCAG
    CCAGACATGG CCATCATGGC TGTCAACAGC TTTGTGAAGG ATTGTGAAGA TCCCAATCCT
    CTGATTCGAG CCTTGGCAGT CAGAACCATG GGGTGCATCC GGGTGGACAA GATTACAGAG
    TATCTCTGTG AGCCCCTCCG CAAGTGCTTA AAGGATGAAG ATCCCTACGT CCGGAAGACA
    GCAGCAGTCT GTGTGGCAAA ACTCCATGAC ATCAATGCCC AGATGGTGGA AGATCAGGGA
    TTTCTGGATT CTCTGCGGGA TCTCATAGCA GATTCAAATC CAATGGTGGT GGCTAATGCT
    GTAGCAGCAC TATCTGAAAT CAGTGAGTCT CACCCCAACA GCAACTTACT CGATCTGAAT
    CCACAGAACA TTAATAAGCT ACTGACAGCC CTGAATGAGT GCACCGAATG GGGCCAGATT
    TTCATCCTGG ACTGCTTATC TAATTACAAT CCTAAAGATG ACCGGGAGGC TCAGAGCATC
    TGTGAGCGGG TAACTCCCCG GTTATCTCAT GCCAACTCAG CAGTGGTGCT TTCAGCAGTA
    AAAGTCCTAA TGAAATTTTT AGAGTTGCTG CCCAAGGACT CTGACTACTA CAGTATGCTG
    CTGAAGAAGT TGGCCCCTCC ACTTGTCACT CTGCTGTCTG GGGAGCCAGA GGTGCAATAC
    GTCGCCCTGA GGAACATCAA CCTAATTGTT CAGAAAAGGC CTGAAATCTT GAAGCAGGAA
    ATAAAAGTTT TCTTTGTGAA GTACAATGAT CCCATCTATG TGAAACTAGA GAAGTTGGAC
    ATCATGATTC GTTTGGCATC CCAAGCCAAC ATTGCTCAGG TTCTGGCTGA ACTGAAAGAG
    TATGCCACAG AGGTGGATGT TGACTTTGTG CGCAAAGCTG TGCGAGCCAT TGGGCGGTGT
    GCCATCAAGG TGGAGCAATC TGCAGAGCGC TGTGTGAGCA CGTTGCTTGA TCTCATCCAA
    ACCAAAGTAA ATTATGTGGT CCAAGAGGCG ATTGTTGTCA TCAGGGACAT TTTCCGCAAA
    TACCCCAACA AATACGAAAG TATCATTGCC ACACTGTGTG AGAACTTAGA TTCTCTGGAT
    GAGCCAGACG CTCGAGCAGC TATGATTTGG ATTGTGGGAG AGTATGCTGA AAGAATTGAC
    AATGCTGATG AGTTACTAGA GAGCTTCCTG GAGGGTTTTC ATGATGAAAG CACCCAAGTG
    CAGCTCACTC TGCTCACTGC CATAGTGAAG CTGTTTCTCA AGAAACCGTC AGAAACACAG
    GAGCTGGTAC AGCAGGTCTT GAGTTTGGCA ACACAGGATT CTGATAATCC TGACCTTCGA
    GACCGGGGCT ATATTTATTG GCGCCTTCTC TCAACTGATC CTGTCACAGC TAAAGAAGTA
    GTCTTGTCTG AAAAGCCACT GATTTCTGAG GAGACAGATC TTATTGAACC AACTCTACTG
    GATGAGCTAA TCTGCCACAT TGGTTCTTTG GCCTCAGTGT ACCATAAGCC GCCCAATGCT
    TTTGTGGAAG GAAGTCATGG AATCCATCGC AAACACTTGC CAATACATCA TGGGAGCACT
    GATGCAGGTG ACAGCCCTGT TGGCGCCGCC ACTGCCACCA ACCTGGAGCA GCCTCAGGTT
    ATTCCCTCCC AGGGTGACCT TCTGGGGGAT CTTTTAAACC TTGACCTTGG TCCCCCAGTC
    AATGTGCCAC AGGTATCTTC CATGCAGATG GGAGCAGTGG ATCTCTTGGG AGGAGGCCTA
    GATAGTCTGC TTGGCAGTGA CCTTGGCGGG GGCATTGGAG GAAGTCCGGC AGTGGGACAG
    TCCTTCATCC CCTCATCAGT GCCTGCAACC TTTGCTCCCT CCCCTACTCC TGCTGTGGTC
    AGCAGTGGTC TGAATGACCT GTTTGAACTC TCCACGGGGA TAGGCATGGC ACCTGGTGGA
    TATGTGGCTC CTAAGGCTGT CTGGCTGCCT GCGGTAAAGG CTAAAGGCCT GGAGATTTCA
    GGAACATTTA CTCACCGCCA AGGGCACATC TATATGGAAA TGAACTTCAC CAACAAAGCT
    CTGCAGCACA TGACAGACTT CGCAATTCAG TTTAACAAGA ATAGCTTTGG TGTCATCCCT
    AGCACTCCTC TGGCCATCCA TACGCCACTG ATGCCAAACC AGAGCATTGA TGTCTCCCTG
    CCCCTCAACA CCTTGGGCCC TGTCATGAAG ATGGAGCCTC TGAACAACCT GCAGGTGGCT
    GTGAAAAACA ATATTGATGT CTTCTACTTC AGCTGCCTCA TCCCACTCAA CGTGCTTTTT
    GTAGAAGATG GCAAAATGGA GCGCCAGGTC TTCCTTGCAA CATGGAAGGA TATTCCCAAT
    GAAAATGAAC TTCAGTTTCA GATTAAGGAA TGTCATTTAA ATGCTGCTGT CGCTGAAGTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.