HIF1A cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	HIF1A
Entrez Gene ID	102392717
Full Name	hypoxia inducible factor 1 alpha subunit
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025295404.1	XP_025151189.1	hypoxia-inducible factor 1-alpha isoform X2
XM_025295406.1	XP_025151191.1	hypoxia-inducible factor 1-alpha isoform X4
XM_025295403.1	XP_025151188.1	hypoxia-inducible factor 1-alpha isoform X1
XM_025295405.1	XP_025151190.1	hypoxia-inducible factor 1-alpha isoform X3
XM_006054432.1	XP_006054494.1	hypoxia-inducible factor 1-alpha isoform X1
XM_006054433.1	XP_006054495.1	hypoxia-inducible factor 1-alpha isoform X2

Pathways from BioSystems
Gene Ontology

The following HIF1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIF1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu11869	XM_006054433.1 Latest version!	Bubalus bubalis hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu11869	XM_025295405.1 Latest version!	Bubalus bubalis hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu11869	XM_006054432.1 Latest version!	Bubalus bubalis hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu44607	XM_025295403.1 Latest version!	Bubalus bubalis hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu44608	XM_025295404.1 Latest version!	Bubalus bubalis hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu44609	XM_025295406.1 Latest version!	Bubalus bubalis hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HIF1A ( XM_025295405.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025151190.1 Bubalus bubalis hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu11869
Clone ID Related Accession (Same CDS sequence)	XM_025295405.1 , XM_006054432.1 , XM_006054433.1
Accession Version	XM_025295405.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	hypoxia-inducible factor 1-alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CCGGGGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG 
AAGTCTAGAG ATGCAGCCAG ATCTCGTCGA AGTAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTCCC CCATAATGTA AGCTCGCATC TTGATAAGGC TTCTGTTATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAG 
CAAAATACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGGGGGAGA 
ACTATGAACA TAAAATCTGC AACATGGAAA GTACTTCACT GCACAGGCCA TATTCATGTA 
TACGATACCA ACAGTAACCA ATCTCAGTGT GGGTATAAGA AACCACCGAT GACATGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAATATTG AAATTCCTTT AGACAGCAAG 
ACTTTTCTCA GTCGTCACAG TCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACA 
GAATTGATGG GATATGAGCC AGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCCTTGGACT CTGACCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACAACAGGGC AGTACAGAAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGAT AGAAACTCAA 
GCAACTGTCA TATATAACAC TAAGAACTCT CAGCCCCAGT GCATTGTATG TGTCAATTAT 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTT 
CTCAAACCAG TTGAATCTTC AGACATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GCAGTCTCTT TGATAAACTT AAGAAGGAGC CTGATGCTTT AACTTTGCTG 
GCCCCAGCTG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAATGA CACAGAGACT 
GATGACCAAC AACTTGAGGA AGTTCCATTG TATAACGATG TAATGCTCCC CTCATCTAAT 
GAGAAATTGC AGAATATAAA TTTGGCGATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG 
CCACTTAGAA GTAGTGCTGA CCCTGCACTC AACCAAGAAG TTGCATTAAA GTTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCTAGT 
CCTTCTGATG GAAGCACCAG ACAAAGTTCA CCTGAGCCTA ACAGTCCCAG TGAATATTGT 
TTTGATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA GAAACTTTTT 
GCTGAAGATA CAGAAGCAAA GAATCCATTT TCCACTCAGG ACACTGATTT AGACTTGGAG 
ATGTTAGCTC CTTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG 
TCACCACTGG AAAACAGTTC TACAAGTCCT CAGAGTGCAA GCACAAATAC AGTATTCCAG 
CCCACTCAAA TGCAAGAACC TCCCATAGCC ACCGTCACTA CCACAGCCAC CAGTGATGAA 
TTGAAAACAG TGACAAAAGA TGGTATGGAA GACATTAAAA TACTGATTGC ATTTCCATCT 
CCTCCCCACG TACCTAAAGA ACCTCCTTGT GCCACAACGT CACCATATAG TGATACTGGA 
AGTCGGACAG CCTCACCCAA CAGAGCAGGA AAAGGAGTCA TAGAACAGAC AGAAAAATCT 
CATCCAAGAA GCCCTAATGT GTTATCTGTC GCTTTGAGTC AAAGAACTAC TGCTCCTGAG 
GAAGAACTAA ATCCAAAGAT ACTAGCTTTG CAGAATGCTC AGAGAAAGCG AAAAATTGAA 
CATGATGGTT CACTTTTTCA AGCAGTAGGA ATTGGAACAT TATTACAGCA ACCAGATGAT 
CGTGCAACTA CCACATCACT TTCTTGGAAA CGTGTAAAAG GATGCAAATC TAGTGAACAA 
AATGGAATGG AGCAAAAGAC AATTATTTTA ATACCCTCTG ATTTAGCATG TAGACTGCTG 
GGGCAATCAA TGGATGAGAG TGGACTACCA CAGCTGACCA GTTATGATTG TGAAGTTAAT 
GCTCCTATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025151190.1
CDS	331..2802
Translation	MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQSQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWIETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSSNEKLQNINLAMSPLPASETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLENSSTSPQSASTNTVFQPTQMQEPPIATVTTTATSDELKTVTKDGMEDIKILIAFPSPPHVPKEPPCATTSPYSDTGSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTAPEEELNPKILALQNAQRKRKIEHDGSLFQAVGIGTLLQQPDDRATTTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_025295405.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025295405.1

ORF Insert Sequence:

ATGGAGGGCG CCGGGGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG 
AAGTCTAGAG ATGCAGCCAG ATCTCGTCGA AGTAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTCCC CCATAATGTA AGCTCGCATC TTGATAAGGC TTCTGTTATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAG 
CAAAATACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGGGGGAGA 
ACTATGAACA TAAAATCTGC AACATGGAAA GTACTTCACT GCACAGGCCA TATTCATGTA 
TACGATACCA ACAGTAACCA ATCTCAGTGT GGGTATAAGA AACCACCGAT GACATGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAATATTG AAATTCCTTT AGACAGCAAG 
ACTTTTCTCA GTCGTCACAG TCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACA 
GAATTGATGG GATATGAGCC AGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCCTTGGACT CTGACCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACAACAGGGC AGTACAGAAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGAT AGAAACTCAA 
GCAACTGTCA TATATAACAC TAAGAACTCT CAGCCCCAGT GCATTGTATG TGTCAATTAT 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTT 
CTCAAACCAG TTGAATCTTC AGACATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GCAGTCTCTT TGATAAACTT AAGAAGGAGC CTGATGCTTT AACTTTGCTG 
GCCCCAGCTG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAATGA CACAGAGACT 
GATGACCAAC AACTTGAGGA AGTTCCATTG TATAACGATG TAATGCTCCC CTCATCTAAT 
GAGAAATTGC AGAATATAAA TTTGGCGATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG 
CCACTTAGAA GTAGTGCTGA CCCTGCACTC AACCAAGAAG TTGCATTAAA GTTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCTAGT 
CCTTCTGATG GAAGCACCAG ACAAAGTTCA CCTGAGCCTA ACAGTCCCAG TGAATATTGT 
TTTGATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA GAAACTTTTT 
GCTGAAGATA CAGAAGCAAA GAATCCATTT TCCACTCAGG ACACTGATTT AGACTTGGAG 
ATGTTAGCTC CTTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG 
TCACCACTGG AAAACAGTTC TACAAGTCCT CAGAGTGCAA GCACAAATAC AGTATTCCAG 
CCCACTCAAA TGCAAGAACC TCCCATAGCC ACCGTCACTA CCACAGCCAC CAGTGATGAA 
TTGAAAACAG TGACAAAAGA TGGTATGGAA GACATTAAAA TACTGATTGC ATTTCCATCT 
CCTCCCCACG TACCTAAAGA ACCTCCTTGT GCCACAACGT CACCATATAG TGATACTGGA 
AGTCGGACAG CCTCACCCAA CAGAGCAGGA AAAGGAGTCA TAGAACAGAC AGAAAAATCT 
CATCCAAGAA GCCCTAATGT GTTATCTGTC GCTTTGAGTC AAAGAACTAC TGCTCCTGAG 
GAAGAACTAA ATCCAAAGAT ACTAGCTTTG CAGAATGCTC AGAGAAAGCG AAAAATTGAA 
CATGATGGTT CACTTTTTCA AGCAGTAGGA ATTGGAACAT TATTACAGCA ACCAGATGAT 
CGTGCAACTA CCACATCACT TTCTTGGAAA CGTGTAAAAG GATGCAAATC TAGTGAACAA 
AATGGAATGG AGCAAAAGAC AATTATTTTA ATACCCTCTG ATTTAGCATG TAGACTGCTG 
GGGCAATCAA TGGATGAGAG TGGACTACCA CAGCTGACCA GTTATGATTG TGAAGTTAAT 
GCTCCTATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIF1A ( XM_006054432.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006054494.1 Bubalus bubalis hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu11869
Clone ID Related Accession (Same CDS sequence)	XM_025295405.1 , XM_006054432.1 , XM_006054433.1
Accession Version	XM_006054432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	hypoxia-inducible factor 1-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784619.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CCGGGGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG 
AAGTCTAGAG ATGCAGCCAG ATCTCGTCGA AGTAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTCCC CCATAATGTA AGCTCGCATC TTGATAAGGC TTCTGTTATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAG 
CAAAATACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGGGGGAGA 
ACTATGAACA TAAAATCTGC AACATGGAAA GTACTTCACT GCACAGGCCA TATTCATGTA 
TACGATACCA ACAGTAACCA ATCTCAGTGT GGGTATAAGA AACCACCGAT GACATGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAATATTG AAATTCCTTT AGACAGCAAG 
ACTTTTCTCA GTCGTCACAG TCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACA 
GAATTGATGG GATATGAGCC AGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCCTTGGACT CTGACCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACAACAGGGC AGTACAGAAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGAT AGAAACTCAA 
GCAACTGTCA TATATAACAC TAAGAACTCT CAGCCCCAGT GCATTGTATG TGTCAATTAT 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTT 
CTCAAACCAG TTGAATCTTC AGACATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GCAGTCTCTT TGATAAACTT AAGAAGGAGC CTGATGCTTT AACTTTGCTG 
GCCCCAGCTG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAATGA CACAGAGACT 
GATGACCAAC AACTTGAGGA AGTTCCATTG TATAACGATG TAATGCTCCC CTCATCTAAT 
GAGAAATTGC AGAATATAAA TTTGGCGATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG 
CCACTTAGAA GTAGTGCTGA CCCTGCACTC AACCAAGAAG TTGCATTAAA GTTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCTAGT 
CCTTCTGATG GAAGCACCAG ACAAAGTTCA CCTGAGCCTA ACAGTCCCAG TGAATATTGT 
TTTGATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA GAAACTTTTT 
GCTGAAGATA CAGAAGCAAA GAATCCATTT TCCACTCAGG ACACTGATTT AGACTTGGAG 
ATGTTAGCTC CTTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG 
TCACCACTGG AAAACAGTTC TACAAGTCCT CAGAGTGCAA GCACAAATAC AGTATTCCAG 
CCCACTCAAA TGCAAGAACC TCCCATAGCC ACCGTCACTA CCACAGCCAC CAGTGATGAA 
TTGAAAACAG TGACAAAAGA TGGTATGGAA GACATTAAAA TACTGATTGC ATTTCCATCT 
CCTCCCCACG TACCTAAAGA ACCTCCTTGT GCCACAACGT CACCATATAG TGATACTGGA 
AGTCGGACAG CCTCACCCAA CAGAGCAGGA AAAGGAGTCA TAGAACAGAC AGAAAAATCT 
CATCCAAGAA GCCCTAATGT GTTATCTGTC GCTTTGAGTC AAAGAACTAC TGCTCCTGAG 
GAAGAACTAA ATCCAAAGAT ACTAGCTTTG CAGAATGCTC AGAGAAAGCG AAAAATTGAA 
CATGATGGTT CACTTTTTCA AGCAGTAGGA ATTGGAACAT TATTACAGCA ACCAGATGAT 
CGTGCAACTA CCACATCACT TTCTTGGAAA CGTGTAAAAG GATGCAAATC TAGTGAACAA 
AATGGAATGG AGCAAAAGAC AATTATTTTA ATACCCTCTG ATTTAGCATG TAGACTGCTG 
GGGCAATCAA TGGATGAGAG TGGACTACCA CAGCTGACCA GTTATGATTG TGAAGTTAAT 
GCTCCTATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006054494.1
CDS	334..2805
Translation	MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQSQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWIETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSSNEKLQNINLAMSPLPASETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLENSSTSPQSASTNTVFQPTQMQEPPIATVTTTATSDELKTVTKDGMEDIKILIAFPSPPHVPKEPPCATTSPYSDTGSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTAPEEELNPKILALQNAQRKRKIEHDGSLFQAVGIGTLLQQPDDRATTTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_006054432.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006054432.1

ORF Insert Sequence:

ATGGAGGGCG CCGGGGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG 
AAGTCTAGAG ATGCAGCCAG ATCTCGTCGA AGTAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTCCC CCATAATGTA AGCTCGCATC TTGATAAGGC TTCTGTTATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAG 
CAAAATACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGGGGGAGA 
ACTATGAACA TAAAATCTGC AACATGGAAA GTACTTCACT GCACAGGCCA TATTCATGTA 
TACGATACCA ACAGTAACCA ATCTCAGTGT GGGTATAAGA AACCACCGAT GACATGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAATATTG AAATTCCTTT AGACAGCAAG 
ACTTTTCTCA GTCGTCACAG TCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACA 
GAATTGATGG GATATGAGCC AGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCCTTGGACT CTGACCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACAACAGGGC AGTACAGAAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGAT AGAAACTCAA 
GCAACTGTCA TATATAACAC TAAGAACTCT CAGCCCCAGT GCATTGTATG TGTCAATTAT 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTT 
CTCAAACCAG TTGAATCTTC AGACATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GCAGTCTCTT TGATAAACTT AAGAAGGAGC CTGATGCTTT AACTTTGCTG 
GCCCCAGCTG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAATGA CACAGAGACT 
GATGACCAAC AACTTGAGGA AGTTCCATTG TATAACGATG TAATGCTCCC CTCATCTAAT 
GAGAAATTGC AGAATATAAA TTTGGCGATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG 
CCACTTAGAA GTAGTGCTGA CCCTGCACTC AACCAAGAAG TTGCATTAAA GTTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCTAGT 
CCTTCTGATG GAAGCACCAG ACAAAGTTCA CCTGAGCCTA ACAGTCCCAG TGAATATTGT 
TTTGATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA GAAACTTTTT 
GCTGAAGATA CAGAAGCAAA GAATCCATTT TCCACTCAGG ACACTGATTT AGACTTGGAG 
ATGTTAGCTC CTTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG 
TCACCACTGG AAAACAGTTC TACAAGTCCT CAGAGTGCAA GCACAAATAC AGTATTCCAG 
CCCACTCAAA TGCAAGAACC TCCCATAGCC ACCGTCACTA CCACAGCCAC CAGTGATGAA 
TTGAAAACAG TGACAAAAGA TGGTATGGAA GACATTAAAA TACTGATTGC ATTTCCATCT 
CCTCCCCACG TACCTAAAGA ACCTCCTTGT GCCACAACGT CACCATATAG TGATACTGGA 
AGTCGGACAG CCTCACCCAA CAGAGCAGGA AAAGGAGTCA TAGAACAGAC AGAAAAATCT 
CATCCAAGAA GCCCTAATGT GTTATCTGTC GCTTTGAGTC AAAGAACTAC TGCTCCTGAG 
GAAGAACTAA ATCCAAAGAT ACTAGCTTTG CAGAATGCTC AGAGAAAGCG AAAAATTGAA 
CATGATGGTT CACTTTTTCA AGCAGTAGGA ATTGGAACAT TATTACAGCA ACCAGATGAT 
CGTGCAACTA CCACATCACT TTCTTGGAAA CGTGTAAAAG GATGCAAATC TAGTGAACAA 
AATGGAATGG AGCAAAAGAC AATTATTTTA ATACCCTCTG ATTTAGCATG TAGACTGCTG 
GGGCAATCAA TGGATGAGAG TGGACTACCA CAGCTGACCA GTTATGATTG TGAAGTTAAT 
GCTCCTATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIF1A ( XM_006054433.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006054495.1 Bubalus bubalis hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu11869
Clone ID Related Accession (Same CDS sequence)	XM_025295405.1 , XM_006054432.1 , XM_006054433.1
Accession Version	XM_006054433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	hypoxia-inducible factor 1-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784619.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CCGGGGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG 
AAGTCTAGAG ATGCAGCCAG ATCTCGTCGA AGTAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTCCC CCATAATGTA AGCTCGCATC TTGATAAGGC TTCTGTTATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAG 
CAAAATACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGGGGGAGA 
ACTATGAACA TAAAATCTGC AACATGGAAA GTACTTCACT GCACAGGCCA TATTCATGTA 
TACGATACCA ACAGTAACCA ATCTCAGTGT GGGTATAAGA AACCACCGAT GACATGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAATATTG AAATTCCTTT AGACAGCAAG 
ACTTTTCTCA GTCGTCACAG TCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACA 
GAATTGATGG GATATGAGCC AGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCCTTGGACT CTGACCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACAACAGGGC AGTACAGAAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGAT AGAAACTCAA 
GCAACTGTCA TATATAACAC TAAGAACTCT CAGCCCCAGT GCATTGTATG TGTCAATTAT 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTT 
CTCAAACCAG TTGAATCTTC AGACATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GCAGTCTCTT TGATAAACTT AAGAAGGAGC CTGATGCTTT AACTTTGCTG 
GCCCCAGCTG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAATGA CACAGAGACT 
GATGACCAAC AACTTGAGGA AGTTCCATTG TATAACGATG TAATGCTCCC CTCATCTAAT 
GAGAAATTGC AGAATATAAA TTTGGCGATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG 
CCACTTAGAA GTAGTGCTGA CCCTGCACTC AACCAAGAAG TTGCATTAAA GTTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCTAGT 
CCTTCTGATG GAAGCACCAG ACAAAGTTCA CCTGAGCCTA ACAGTCCCAG TGAATATTGT 
TTTGATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA GAAACTTTTT 
GCTGAAGATA CAGAAGCAAA GAATCCATTT TCCACTCAGG ACACTGATTT AGACTTGGAG 
ATGTTAGCTC CTTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG 
TCACCACTGG AAAACAGTTC TACAAGTCCT CAGAGTGCAA GCACAAATAC AGTATTCCAG 
CCCACTCAAA TGCAAGAACC TCCCATAGCC ACCGTCACTA CCACAGCCAC CAGTGATGAA 
TTGAAAACAG TGACAAAAGA TGGTATGGAA GACATTAAAA TACTGATTGC ATTTCCATCT 
CCTCCCCACG TACCTAAAGA ACCTCCTTGT GCCACAACGT CACCATATAG TGATACTGGA 
AGTCGGACAG CCTCACCCAA CAGAGCAGGA AAAGGAGTCA TAGAACAGAC AGAAAAATCT 
CATCCAAGAA GCCCTAATGT GTTATCTGTC GCTTTGAGTC AAAGAACTAC TGCTCCTGAG 
GAAGAACTAA ATCCAAAGAT ACTAGCTTTG CAGAATGCTC AGAGAAAGCG AAAAATTGAA 
CATGATGGTT CACTTTTTCA AGCAGTAGGA ATTGGAACAT TATTACAGCA ACCAGATGAT 
CGTGCAACTA CCACATCACT TTCTTGGAAA CGTGTAAAAG GATGCAAATC TAGTGAACAA 
AATGGAATGG AGCAAAAGAC AATTATTTTA ATACCCTCTG ATTTAGCATG TAGACTGCTG 
GGGCAATCAA TGGATGAGAG TGGACTACCA CAGCTGACCA GTTATGATTG TGAAGTTAAT 
GCTCCTATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006054495.1
CDS	334..2805
Translation	MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQSQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWIETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSSNEKLQNINLAMSPLPASETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLENSSTSPQSASTNTVFQPTQMQEPPIATVTTTATSDELKTVTKDGMEDIKILIAFPSPPHVPKEPPCATTSPYSDTGSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTAPEEELNPKILALQNAQRKRKIEHDGSLFQAVGIGTLLQQPDDRATTTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_006054433.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006054433.1

ORF Insert Sequence:

ATGGAGGGCG CCGGGGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG 
AAGTCTAGAG ATGCAGCCAG ATCTCGTCGA AGTAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTCCC CCATAATGTA AGCTCGCATC TTGATAAGGC TTCTGTTATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAG 
CAAAATACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGGGGGAGA 
ACTATGAACA TAAAATCTGC AACATGGAAA GTACTTCACT GCACAGGCCA TATTCATGTA 
TACGATACCA ACAGTAACCA ATCTCAGTGT GGGTATAAGA AACCACCGAT GACATGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAATATTG AAATTCCTTT AGACAGCAAG 
ACTTTTCTCA GTCGTCACAG TCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACA 
GAATTGATGG GATATGAGCC AGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCCTTGGACT CTGACCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACAACAGGGC AGTACAGAAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGAT AGAAACTCAA 
GCAACTGTCA TATATAACAC TAAGAACTCT CAGCCCCAGT GCATTGTATG TGTCAATTAT 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTT 
CTCAAACCAG TTGAATCTTC AGACATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GCAGTCTCTT TGATAAACTT AAGAAGGAGC CTGATGCTTT AACTTTGCTG 
GCCCCAGCTG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAATGA CACAGAGACT 
GATGACCAAC AACTTGAGGA AGTTCCATTG TATAACGATG TAATGCTCCC CTCATCTAAT 
GAGAAATTGC AGAATATAAA TTTGGCGATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG 
CCACTTAGAA GTAGTGCTGA CCCTGCACTC AACCAAGAAG TTGCATTAAA GTTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCTAGT 
CCTTCTGATG GAAGCACCAG ACAAAGTTCA CCTGAGCCTA ACAGTCCCAG TGAATATTGT 
TTTGATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA GAAACTTTTT 
GCTGAAGATA CAGAAGCAAA GAATCCATTT TCCACTCAGG ACACTGATTT AGACTTGGAG 
ATGTTAGCTC CTTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG 
TCACCACTGG AAAACAGTTC TACAAGTCCT CAGAGTGCAA GCACAAATAC AGTATTCCAG 
CCCACTCAAA TGCAAGAACC TCCCATAGCC ACCGTCACTA CCACAGCCAC CAGTGATGAA 
TTGAAAACAG TGACAAAAGA TGGTATGGAA GACATTAAAA TACTGATTGC ATTTCCATCT 
CCTCCCCACG TACCTAAAGA ACCTCCTTGT GCCACAACGT CACCATATAG TGATACTGGA 
AGTCGGACAG CCTCACCCAA CAGAGCAGGA AAAGGAGTCA TAGAACAGAC AGAAAAATCT 
CATCCAAGAA GCCCTAATGT GTTATCTGTC GCTTTGAGTC AAAGAACTAC TGCTCCTGAG 
GAAGAACTAA ATCCAAAGAT ACTAGCTTTG CAGAATGCTC AGAGAAAGCG AAAAATTGAA 
CATGATGGTT CACTTTTTCA AGCAGTAGGA ATTGGAACAT TATTACAGCA ACCAGATGAT 
CGTGCAACTA CCACATCACT TTCTTGGAAA CGTGTAAAAG GATGCAAATC TAGTGAACAA 
AATGGAATGG AGCAAAAGAC AATTATTTTA ATACCCTCTG ATTTAGCATG TAGACTGCTG 
GGGCAATCAA TGGATGAGAG TGGACTACCA CAGCTGACCA GTTATGATTG TGAAGTTAAT 
GCTCCTATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIF1A ( XM_025295403.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025151188.1 Bubalus bubalis hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu44607
Clone ID Related Accession (Same CDS sequence)	XM_025295403.1
Accession Version	XM_025295403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	hypoxia-inducible factor 1-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CCGGGGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG 
AAGTCTAGAG ATGCAGCCAG ATCTCGTCGA AGTAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTCCC CCATAATGTA AGCTCGCATC TTGATAAGGC TTCTGTTATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGGA ATCACTGCTA TTGTGAACCC 
GTTACTTACT ACAAGTGCAT TAAATCCCTT GGATGTATTG GAGTGAGCAA AGCAGGCCTT 
GTGAAAAAGG GTAAAGAGCA AAATACACAG CGGAGCTTTT TTCTCAGAAT GAAGTGTACC 
CTAACTAGCC GGGGGAGAAC TATGAACATA AAATCTGCAA CATGGAAAGT ACTTCACTGC 
ACAGGCCATA TTCATGTATA CGATACCAAC AGTAACCAAT CTCAGTGTGG GTATAAGAAA 
CCACCGATGA CATGCTTGGT GCTGATTTGT GAACCCATTC CTCATCCATC AAATATTGAA 
ATTCCTTTAG ACAGCAAGAC TTTTCTCAGT CGTCACAGTC TGGATATGAA ATTTTCTTAT 
TGTGATGAAA GAATTACAGA ATTGATGGGA TATGAGCCAG AAGAACTTTT GGGCCGCTCA 
ATTTATGAAT ATTATCATGC CTTGGACTCT GACCATCTGA CCAAAACTCA TCATGATATG 
TTTACTAAAG GACAAGTCAC AACAGGGCAG TACAGAATGC TTGCCAAAAG AGGTGGATAT 
GTCTGGATAG AAACTCAAGC AACTGTCATA TATAACACTA AGAACTCTCA GCCCCAGTGC 
ATTGTATGTG TCAATTATGT TGTGAGTGGT ATTATTCAGC ACGACTTGAT TTTCTCCCTT 
CAACAAACAG AATGTGTTCT CAAACCAGTT GAATCTTCAG ACATGAAAAT GACTCAGCTA 
TTCACCAAAG TTGAATCAGA AGATACAAGC AGTCTCTTTG ATAAACTTAA GAAGGAGCCT 
GATGCTTTAA CTTTGCTGGC CCCAGCTGCT GGAGACACAA TCATATCTTT AGATTTTGGC 
AGCAATGACA CAGAGACTGA TGACCAACAA CTTGAGGAAG TTCCATTGTA TAACGATGTA 
ATGCTCCCCT CATCTAATGA GAAATTGCAG AATATAAATT TGGCGATGTC TCCATTACCT 
GCCTCTGAAA CTCCAAAGCC ACTTAGAAGT AGTGCTGACC CTGCACTCAA CCAAGAAGTT 
GCATTAAAGT TAGAACCAAA TCCAGAGTCA CTGGAACTTT CTTTTACCAT GCCCCAGATT 
CAAGATCAGC CAGCTAGTCC TTCTGATGGA AGCACCAGAC AAAGTTCACC TGAGCCTAAC 
AGTCCCAGTG AATATTGTTT TGATGTGGAT AGTGATATGG TCAATGAATT CAAGTTGGAA 
TTGGTAGAGA AACTTTTTGC TGAAGATACA GAAGCAAAGA ATCCATTTTC CACTCAGGAC 
ACTGATTTAG ACTTGGAGAT GTTAGCTCCT TATATCCCAA TGGATGATGA CTTCCAGTTA 
CGTTCCTTCG ATCAGTTGTC ACCACTGGAA AACAGTTCTA CAAGTCCTCA GAGTGCAAGC 
ACAAATACAG TATTCCAGCC CACTCAAATG CAAGAACCTC CCATAGCCAC CGTCACTACC 
ACAGCCACCA GTGATGAATT GAAAACAGTG ACAAAAGATG GTATGGAAGA CATTAAAATA 
CTGATTGCAT TTCCATCTCC TCCCCACGTA CCTAAAGAAC CTCCTTGTGC CACAACGTCA 
CCATATAGTG ATACTGGAAG TCGGACAGCC TCACCCAACA GAGCAGGAAA AGGAGTCATA 
GAACAGACAG AAAAATCTCA TCCAAGAAGC CCTAATGTGT TATCTGTCGC TTTGAGTCAA 
AGAACTACTG CTCCTGAGGA AGAACTAAAT CCAAAGATAC TAGCTTTGCA GAATGCTCAG 
AGAAAGCGAA AAATTGAACA TGATGGTTCA CTTTTTCAAG CAGTAGGAAT TGGAACATTA 
TTACAGCAAC CAGATGATCG TGCAACTACC ACATCACTTT CTTGGAAACG TGTAAAAGGA 
TGCAAATCTA GTGAACAAAA TGGAATGGAG CAAAAGACAA TTATTTTAAT ACCCTCTGAT 
TTAGCATGTA GACTGCTGGG GCAATCAATG GATGAGAGTG GACTACCACA GCTGACCAGT 
TATGATTGTG AAGTTAATGC TCCTATACAA GGCAGCAGAA ACCTACTGCA GGGTGAAGAA 
TTACTCAGAG CTTTGGATCA AGTTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025151188.1
CDS	332..2881
Translation	MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGNHCYCEPVTYYKCIKSLGCIGVSKAGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQSQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWIETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSSNEKLQNINLAMSPLPASETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLENSSTSPQSASTNTVFQPTQMQEPPIATVTTTATSDELKTVTKDGMEDIKILIAFPSPPHVPKEPPCATTSPYSDTGSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTAPEEELNPKILALQNAQRKRKIEHDGSLFQAVGIGTLLQQPDDRATTTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_025295403.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025295403.1

ORF Insert Sequence:

ATGGAGGGCG CCGGGGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG 
AAGTCTAGAG ATGCAGCCAG ATCTCGTCGA AGTAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTCCC CCATAATGTA AGCTCGCATC TTGATAAGGC TTCTGTTATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGGA ATCACTGCTA TTGTGAACCC 
GTTACTTACT ACAAGTGCAT TAAATCCCTT GGATGTATTG GAGTGAGCAA AGCAGGCCTT 
GTGAAAAAGG GTAAAGAGCA AAATACACAG CGGAGCTTTT TTCTCAGAAT GAAGTGTACC 
CTAACTAGCC GGGGGAGAAC TATGAACATA AAATCTGCAA CATGGAAAGT ACTTCACTGC 
ACAGGCCATA TTCATGTATA CGATACCAAC AGTAACCAAT CTCAGTGTGG GTATAAGAAA 
CCACCGATGA CATGCTTGGT GCTGATTTGT GAACCCATTC CTCATCCATC AAATATTGAA 
ATTCCTTTAG ACAGCAAGAC TTTTCTCAGT CGTCACAGTC TGGATATGAA ATTTTCTTAT 
TGTGATGAAA GAATTACAGA ATTGATGGGA TATGAGCCAG AAGAACTTTT GGGCCGCTCA 
ATTTATGAAT ATTATCATGC CTTGGACTCT GACCATCTGA CCAAAACTCA TCATGATATG 
TTTACTAAAG GACAAGTCAC AACAGGGCAG TACAGAATGC TTGCCAAAAG AGGTGGATAT 
GTCTGGATAG AAACTCAAGC AACTGTCATA TATAACACTA AGAACTCTCA GCCCCAGTGC 
ATTGTATGTG TCAATTATGT TGTGAGTGGT ATTATTCAGC ACGACTTGAT TTTCTCCCTT 
CAACAAACAG AATGTGTTCT CAAACCAGTT GAATCTTCAG ACATGAAAAT GACTCAGCTA 
TTCACCAAAG TTGAATCAGA AGATACAAGC AGTCTCTTTG ATAAACTTAA GAAGGAGCCT 
GATGCTTTAA CTTTGCTGGC CCCAGCTGCT GGAGACACAA TCATATCTTT AGATTTTGGC 
AGCAATGACA CAGAGACTGA TGACCAACAA CTTGAGGAAG TTCCATTGTA TAACGATGTA 
ATGCTCCCCT CATCTAATGA GAAATTGCAG AATATAAATT TGGCGATGTC TCCATTACCT 
GCCTCTGAAA CTCCAAAGCC ACTTAGAAGT AGTGCTGACC CTGCACTCAA CCAAGAAGTT 
GCATTAAAGT TAGAACCAAA TCCAGAGTCA CTGGAACTTT CTTTTACCAT GCCCCAGATT 
CAAGATCAGC CAGCTAGTCC TTCTGATGGA AGCACCAGAC AAAGTTCACC TGAGCCTAAC 
AGTCCCAGTG AATATTGTTT TGATGTGGAT AGTGATATGG TCAATGAATT CAAGTTGGAA 
TTGGTAGAGA AACTTTTTGC TGAAGATACA GAAGCAAAGA ATCCATTTTC CACTCAGGAC 
ACTGATTTAG ACTTGGAGAT GTTAGCTCCT TATATCCCAA TGGATGATGA CTTCCAGTTA 
CGTTCCTTCG ATCAGTTGTC ACCACTGGAA AACAGTTCTA CAAGTCCTCA GAGTGCAAGC 
ACAAATACAG TATTCCAGCC CACTCAAATG CAAGAACCTC CCATAGCCAC CGTCACTACC 
ACAGCCACCA GTGATGAATT GAAAACAGTG ACAAAAGATG GTATGGAAGA CATTAAAATA 
CTGATTGCAT TTCCATCTCC TCCCCACGTA CCTAAAGAAC CTCCTTGTGC CACAACGTCA 
CCATATAGTG ATACTGGAAG TCGGACAGCC TCACCCAACA GAGCAGGAAA AGGAGTCATA 
GAACAGACAG AAAAATCTCA TCCAAGAAGC CCTAATGTGT TATCTGTCGC TTTGAGTCAA 
AGAACTACTG CTCCTGAGGA AGAACTAAAT CCAAAGATAC TAGCTTTGCA GAATGCTCAG 
AGAAAGCGAA AAATTGAACA TGATGGTTCA CTTTTTCAAG CAGTAGGAAT TGGAACATTA 
TTACAGCAAC CAGATGATCG TGCAACTACC ACATCACTTT CTTGGAAACG TGTAAAAGGA 
TGCAAATCTA GTGAACAAAA TGGAATGGAG CAAAAGACAA TTATTTTAAT ACCCTCTGAT 
TTAGCATGTA GACTGCTGGG GCAATCAATG GATGAGAGTG GACTACCACA GCTGACCAGT 
TATGATTGTG AAGTTAATGC TCCTATACAA GGCAGCAGAA ACCTACTGCA GGGTGAAGAA 
TTACTCAGAG CTTTGGATCA AGTTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIF1A ( XM_025295404.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025151189.1 Bubalus bubalis hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu44608
Clone ID Related Accession (Same CDS sequence)	XM_025295404.1
Accession Version	XM_025295404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	hypoxia-inducible factor 1-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCTTC ACCAGATAAG TTCTGAACGT CGAAAAGAGA AGTCTAGAGA TGCAGCCAGA 
TCTCGTCGAA GTAAAGAGTC TGAAGTTTTT TATGAGCTTG CTCATCAGTT GCCACTCCCC 
CATAATGTAA GCTCGCATCT TGATAAGGCT TCTGTTATGA GGCTTACCAT CAGCTATTTG 
CGTGTGAGGA AACTTCTGGA TGCTGGTGAT TTGGATATTG AAGATGAAAT GAAGGCACAG 
ATGAATTGCT TTTATTTGAA AGCCTTGGAT GGTTTTGTTA TGGTTCTCAC AGATGATGGT 
GACATGATTT ACATTTCTGA TAATGTGAAC AAATACATGG GATTAACTCA GTTTGAACTA 
ACTGGACACA GTGTGTTTGA TTTTACTCAT CCATGTGACC ATGAGGAAAT GAGAGAAATG 
CTTACACACA GAAATGGGAA TCACTGCTAT TGTGAACCCG TTACTTACTA CAAGTGCATT 
AAATCCCTTG GATGTATTGG AGTGAGCAAA GCAGGCCTTG TGAAAAAGGG TAAAGAGCAA 
AATACACAGC GGAGCTTTTT TCTCAGAATG AAGTGTACCC TAACTAGCCG GGGGAGAACT 
ATGAACATAA AATCTGCAAC ATGGAAAGTA CTTCACTGCA CAGGCCATAT TCATGTATAC 
GATACCAACA GTAACCAATC TCAGTGTGGG TATAAGAAAC CACCGATGAC ATGCTTGGTG 
CTGATTTGTG AACCCATTCC TCATCCATCA AATATTGAAA TTCCTTTAGA CAGCAAGACT 
TTTCTCAGTC GTCACAGTCT GGATATGAAA TTTTCTTATT GTGATGAAAG AATTACAGAA 
TTGATGGGAT ATGAGCCAGA AGAACTTTTG GGCCGCTCAA TTTATGAATA TTATCATGCC 
TTGGACTCTG ACCATCTGAC CAAAACTCAT CATGATATGT TTACTAAAGG ACAAGTCACA 
ACAGGGCAGT ACAGAATGCT TGCCAAAAGA GGTGGATATG TCTGGATAGA AACTCAAGCA 
ACTGTCATAT ATAACACTAA GAACTCTCAG CCCCAGTGCA TTGTATGTGT CAATTATGTT 
GTGAGTGGTA TTATTCAGCA CGACTTGATT TTCTCCCTTC AACAAACAGA ATGTGTTCTC 
AAACCAGTTG AATCTTCAGA CATGAAAATG ACTCAGCTAT TCACCAAAGT TGAATCAGAA 
GATACAAGCA GTCTCTTTGA TAAACTTAAG AAGGAGCCTG ATGCTTTAAC TTTGCTGGCC 
CCAGCTGCTG GAGACACAAT CATATCTTTA GATTTTGGCA GCAATGACAC AGAGACTGAT 
GACCAACAAC TTGAGGAAGT TCCATTGTAT AACGATGTAA TGCTCCCCTC ATCTAATGAG 
AAATTGCAGA ATATAAATTT GGCGATGTCT CCATTACCTG CCTCTGAAAC TCCAAAGCCA 
CTTAGAAGTA GTGCTGACCC TGCACTCAAC CAAGAAGTTG CATTAAAGTT AGAACCAAAT 
CCAGAGTCAC TGGAACTTTC TTTTACCATG CCCCAGATTC AAGATCAGCC AGCTAGTCCT 
TCTGATGGAA GCACCAGACA AAGTTCACCT GAGCCTAACA GTCCCAGTGA ATATTGTTTT 
GATGTGGATA GTGATATGGT CAATGAATTC AAGTTGGAAT TGGTAGAGAA ACTTTTTGCT 
GAAGATACAG AAGCAAAGAA TCCATTTTCC ACTCAGGACA CTGATTTAGA CTTGGAGATG 
TTAGCTCCTT ATATCCCAAT GGATGATGAC TTCCAGTTAC GTTCCTTCGA TCAGTTGTCA 
CCACTGGAAA ACAGTTCTAC AAGTCCTCAG AGTGCAAGCA CAAATACAGT ATTCCAGCCC 
ACTCAAATGC AAGAACCTCC CATAGCCACC GTCACTACCA CAGCCACCAG TGATGAATTG 
AAAACAGTGA CAAAAGATGG TATGGAAGAC ATTAAAATAC TGATTGCATT TCCATCTCCT 
CCCCACGTAC CTAAAGAACC TCCTTGTGCC ACAACGTCAC CATATAGTGA TACTGGAAGT 
CGGACAGCCT CACCCAACAG AGCAGGAAAA GGAGTCATAG AACAGACAGA AAAATCTCAT 
CCAAGAAGCC CTAATGTGTT ATCTGTCGCT TTGAGTCAAA GAACTACTGC TCCTGAGGAA 
GAACTAAATC CAAAGATACT AGCTTTGCAG AATGCTCAGA GAAAGCGAAA AATTGAACAT 
GATGGTTCAC TTTTTCAAGC AGTAGGAATT GGAACATTAT TACAGCAACC AGATGATCGT 
GCAACTACCA CATCACTTTC TTGGAAACGT GTAAAAGGAT GCAAATCTAG TGAACAAAAT 
GGAATGGAGC AAAAGACAAT TATTTTAATA CCCTCTGATT TAGCATGTAG ACTGCTGGGG 
CAATCAATGG ATGAGAGTGG ACTACCACAG CTGACCAGTT ATGATTGTGA AGTTAATGCT 
CCTATACAAG GCAGCAGAAA CCTACTGCAG GGTGAAGAAT TACTCAGAGC TTTGGATCAA 
GTTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025151189.1
CDS	31..2559
Translation	MHLHQISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGNHCYCEPVTYYKCIKSLGCIGVSKAGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQSQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWIETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSSNEKLQNINLAMSPLPASETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLENSSTSPQSASTNTVFQPTQMQEPPIATVTTTATSDELKTVTKDGMEDIKILIAFPSPPHVPKEPPCATTSPYSDTGSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTAPEEELNPKILALQNAQRKRKIEHDGSLFQAVGIGTLLQQPDDRATTTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_025295404.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025295404.1

ORF Insert Sequence:

ATGCATCTTC ACCAGATAAG TTCTGAACGT CGAAAAGAGA AGTCTAGAGA TGCAGCCAGA 
TCTCGTCGAA GTAAAGAGTC TGAAGTTTTT TATGAGCTTG CTCATCAGTT GCCACTCCCC 
CATAATGTAA GCTCGCATCT TGATAAGGCT TCTGTTATGA GGCTTACCAT CAGCTATTTG 
CGTGTGAGGA AACTTCTGGA TGCTGGTGAT TTGGATATTG AAGATGAAAT GAAGGCACAG 
ATGAATTGCT TTTATTTGAA AGCCTTGGAT GGTTTTGTTA TGGTTCTCAC AGATGATGGT 
GACATGATTT ACATTTCTGA TAATGTGAAC AAATACATGG GATTAACTCA GTTTGAACTA 
ACTGGACACA GTGTGTTTGA TTTTACTCAT CCATGTGACC ATGAGGAAAT GAGAGAAATG 
CTTACACACA GAAATGGGAA TCACTGCTAT TGTGAACCCG TTACTTACTA CAAGTGCATT 
AAATCCCTTG GATGTATTGG AGTGAGCAAA GCAGGCCTTG TGAAAAAGGG TAAAGAGCAA 
AATACACAGC GGAGCTTTTT TCTCAGAATG AAGTGTACCC TAACTAGCCG GGGGAGAACT 
ATGAACATAA AATCTGCAAC ATGGAAAGTA CTTCACTGCA CAGGCCATAT TCATGTATAC 
GATACCAACA GTAACCAATC TCAGTGTGGG TATAAGAAAC CACCGATGAC ATGCTTGGTG 
CTGATTTGTG AACCCATTCC TCATCCATCA AATATTGAAA TTCCTTTAGA CAGCAAGACT 
TTTCTCAGTC GTCACAGTCT GGATATGAAA TTTTCTTATT GTGATGAAAG AATTACAGAA 
TTGATGGGAT ATGAGCCAGA AGAACTTTTG GGCCGCTCAA TTTATGAATA TTATCATGCC 
TTGGACTCTG ACCATCTGAC CAAAACTCAT CATGATATGT TTACTAAAGG ACAAGTCACA 
ACAGGGCAGT ACAGAATGCT TGCCAAAAGA GGTGGATATG TCTGGATAGA AACTCAAGCA 
ACTGTCATAT ATAACACTAA GAACTCTCAG CCCCAGTGCA TTGTATGTGT CAATTATGTT 
GTGAGTGGTA TTATTCAGCA CGACTTGATT TTCTCCCTTC AACAAACAGA ATGTGTTCTC 
AAACCAGTTG AATCTTCAGA CATGAAAATG ACTCAGCTAT TCACCAAAGT TGAATCAGAA 
GATACAAGCA GTCTCTTTGA TAAACTTAAG AAGGAGCCTG ATGCTTTAAC TTTGCTGGCC 
CCAGCTGCTG GAGACACAAT CATATCTTTA GATTTTGGCA GCAATGACAC AGAGACTGAT 
GACCAACAAC TTGAGGAAGT TCCATTGTAT AACGATGTAA TGCTCCCCTC ATCTAATGAG 
AAATTGCAGA ATATAAATTT GGCGATGTCT CCATTACCTG CCTCTGAAAC TCCAAAGCCA 
CTTAGAAGTA GTGCTGACCC TGCACTCAAC CAAGAAGTTG CATTAAAGTT AGAACCAAAT 
CCAGAGTCAC TGGAACTTTC TTTTACCATG CCCCAGATTC AAGATCAGCC AGCTAGTCCT 
TCTGATGGAA GCACCAGACA AAGTTCACCT GAGCCTAACA GTCCCAGTGA ATATTGTTTT 
GATGTGGATA GTGATATGGT CAATGAATTC AAGTTGGAAT TGGTAGAGAA ACTTTTTGCT 
GAAGATACAG AAGCAAAGAA TCCATTTTCC ACTCAGGACA CTGATTTAGA CTTGGAGATG 
TTAGCTCCTT ATATCCCAAT GGATGATGAC TTCCAGTTAC GTTCCTTCGA TCAGTTGTCA 
CCACTGGAAA ACAGTTCTAC AAGTCCTCAG AGTGCAAGCA CAAATACAGT ATTCCAGCCC 
ACTCAAATGC AAGAACCTCC CATAGCCACC GTCACTACCA CAGCCACCAG TGATGAATTG 
AAAACAGTGA CAAAAGATGG TATGGAAGAC ATTAAAATAC TGATTGCATT TCCATCTCCT 
CCCCACGTAC CTAAAGAACC TCCTTGTGCC ACAACGTCAC CATATAGTGA TACTGGAAGT 
CGGACAGCCT CACCCAACAG AGCAGGAAAA GGAGTCATAG AACAGACAGA AAAATCTCAT 
CCAAGAAGCC CTAATGTGTT ATCTGTCGCT TTGAGTCAAA GAACTACTGC TCCTGAGGAA 
GAACTAAATC CAAAGATACT AGCTTTGCAG AATGCTCAGA GAAAGCGAAA AATTGAACAT 
GATGGTTCAC TTTTTCAAGC AGTAGGAATT GGAACATTAT TACAGCAACC AGATGATCGT 
GCAACTACCA CATCACTTTC TTGGAAACGT GTAAAAGGAT GCAAATCTAG TGAACAAAAT 
GGAATGGAGC AAAAGACAAT TATTTTAATA CCCTCTGATT TAGCATGTAG ACTGCTGGGG 
CAATCAATGG ATGAGAGTGG ACTACCACAG CTGACCAGTT ATGATTGTGA AGTTAATGCT 
CCTATACAAG GCAGCAGAAA CCTACTGCAG GGTGAAGAAT TACTCAGAGC TTTGGATCAA 
GTTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIF1A ( XM_025295406.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025151191.1 Bubalus bubalis hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X5, mRNA.

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CloneID	OBu44609
Clone ID Related Accession (Same CDS sequence)	XM_025295406.1
Accession Version	XM_025295406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	hypoxia-inducible factor 1-alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGAG GAAATCAAGT TGCTCTTTGG AATCACTGCT ATTGTGAACC CGTTACTTAC 
TACAAGTGCA TTAAATCCCT TGGATGTATT GGAGTGAGCA AAGCAGGCCT TGTGAAAAAG 
GGTAAAGAGC AAAATACACA GCGGAGCTTT TTTCTCAGAA TGAAGTGTAC CCTAACTAGC 
CGGGGGAGAA CTATGAACAT AAAATCTGCA ACATGGAAAG TACTTCACTG CACAGGCCAT 
ATTCATGTAT ACGATACCAA CAGTAACCAA TCTCAGTGTG GGTATAAGAA ACCACCGATG 
ACATGCTTGG TGCTGATTTG TGAACCCATT CCTCATCCAT CAAATATTGA AATTCCTTTA 
GACAGCAAGA CTTTTCTCAG TCGTCACAGT CTGGATATGA AATTTTCTTA TTGTGATGAA 
AGAATTACAG AATTGATGGG ATATGAGCCA GAAGAACTTT TGGGCCGCTC AATTTATGAA 
TATTATCATG CCTTGGACTC TGACCATCTG ACCAAAACTC ATCATGATAT GTTTACTAAA 
GGACAAGTCA CAACAGGGCA GTACAGAATG CTTGCCAAAA GAGGTGGATA TGTCTGGATA 
GAAACTCAAG CAACTGTCAT ATATAACACT AAGAACTCTC AGCCCCAGTG CATTGTATGT 
GTCAATTATG TTGTGAGTGG TATTATTCAG CACGACTTGA TTTTCTCCCT TCAACAAACA 
GAATGTGTTC TCAAACCAGT TGAATCTTCA GACATGAAAA TGACTCAGCT ATTCACCAAA 
GTTGAATCAG AAGATACAAG CAGTCTCTTT GATAAACTTA AGAAGGAGCC TGATGCTTTA 
ACTTTGCTGG CCCCAGCTGC TGGAGACACA ATCATATCTT TAGATTTTGG CAGCAATGAC 
ACAGAGACTG ATGACCAACA ACTTGAGGAA GTTCCATTGT ATAACGATGT AATGCTCCCC 
TCATCTAATG AGAAATTGCA GAATATAAAT TTGGCGATGT CTCCATTACC TGCCTCTGAA 
ACTCCAAAGC CACTTAGAAG TAGTGCTGAC CCTGCACTCA ACCAAGAAGT TGCATTAAAG 
TTAGAACCAA ATCCAGAGTC ACTGGAACTT TCTTTTACCA TGCCCCAGAT TCAAGATCAG 
CCAGCTAGTC CTTCTGATGG AAGCACCAGA CAAAGTTCAC CTGAGCCTAA CAGTCCCAGT 
GAATATTGTT TTGATGTGGA TAGTGATATG GTCAATGAAT TCAAGTTGGA ATTGGTAGAG 
AAACTTTTTG CTGAAGATAC AGAAGCAAAG AATCCATTTT CCACTCAGGA CACTGATTTA 
GACTTGGAGA TGTTAGCTCC TTATATCCCA ATGGATGATG ACTTCCAGTT ACGTTCCTTC 
GATCAGTTGT CACCACTGGA AAACAGTTCT ACAAGTCCTC AGAGTGCAAG CACAAATACA 
GTATTCCAGC CCACTCAAAT GCAAGAACCT CCCATAGCCA CCGTCACTAC CACAGCCACC 
AGTGATGAAT TGAAAACAGT GACAAAAGAT GGTATGGAAG ACATTAAAAT ACTGATTGCA 
TTTCCATCTC CTCCCCACGT ACCTAAAGAA CCTCCTTGTG CCACAACGTC ACCATATAGT 
GATACTGGAA GTCGGACAGC CTCACCCAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA 
GAAAAATCTC ATCCAAGAAG CCCTAATGTG TTATCTGTCG CTTTGAGTCA AAGAACTACT 
GCTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA 
AAAATTGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTGGAACATT ATTACAGCAA 
CCAGATGATC GTGCAACTAC CACATCACTT TCTTGGAAAC GTGTAAAAGG ATGCAAATCT 
AGTGAACAAA ATGGAATGGA GCAAAAGACA ATTATTTTAA TACCCTCTGA TTTAGCATGT 
AGACTGCTGG GGCAATCAAT GGATGAGAGT GGACTACCAC AGCTGACCAG TTATGATTGT 
GAAGTTAATG CTCCTATACA AGGCAGCAGA AACCTACTGC AGGGTGAAGA ATTACTCAGA 
GCTTTGGATC AAGTTAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025151191.1
CDS	98..2218
Translation	MKRGNQVALWNHCYCEPVTYYKCIKSLGCIGVSKAGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQSQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWIETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSSNEKLQNINLAMSPLPASETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLENSSTSPQSASTNTVFQPTQMQEPPIATVTTTATSDELKTVTKDGMEDIKILIAFPSPPHVPKEPPCATTSPYSDTGSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTAPEEELNPKILALQNAQRKRKIEHDGSLFQAVGIGTLLQQPDDRATTTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_025295406.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025295406.1

ORF Insert Sequence:

ATGAAGAGAG GAAATCAAGT TGCTCTTTGG AATCACTGCT ATTGTGAACC CGTTACTTAC 
TACAAGTGCA TTAAATCCCT TGGATGTATT GGAGTGAGCA AAGCAGGCCT TGTGAAAAAG 
GGTAAAGAGC AAAATACACA GCGGAGCTTT TTTCTCAGAA TGAAGTGTAC CCTAACTAGC 
CGGGGGAGAA CTATGAACAT AAAATCTGCA ACATGGAAAG TACTTCACTG CACAGGCCAT 
ATTCATGTAT ACGATACCAA CAGTAACCAA TCTCAGTGTG GGTATAAGAA ACCACCGATG 
ACATGCTTGG TGCTGATTTG TGAACCCATT CCTCATCCAT CAAATATTGA AATTCCTTTA 
GACAGCAAGA CTTTTCTCAG TCGTCACAGT CTGGATATGA AATTTTCTTA TTGTGATGAA 
AGAATTACAG AATTGATGGG ATATGAGCCA GAAGAACTTT TGGGCCGCTC AATTTATGAA 
TATTATCATG CCTTGGACTC TGACCATCTG ACCAAAACTC ATCATGATAT GTTTACTAAA 
GGACAAGTCA CAACAGGGCA GTACAGAATG CTTGCCAAAA GAGGTGGATA TGTCTGGATA 
GAAACTCAAG CAACTGTCAT ATATAACACT AAGAACTCTC AGCCCCAGTG CATTGTATGT 
GTCAATTATG TTGTGAGTGG TATTATTCAG CACGACTTGA TTTTCTCCCT TCAACAAACA 
GAATGTGTTC TCAAACCAGT TGAATCTTCA GACATGAAAA TGACTCAGCT ATTCACCAAA 
GTTGAATCAG AAGATACAAG CAGTCTCTTT GATAAACTTA AGAAGGAGCC TGATGCTTTA 
ACTTTGCTGG CCCCAGCTGC TGGAGACACA ATCATATCTT TAGATTTTGG CAGCAATGAC 
ACAGAGACTG ATGACCAACA ACTTGAGGAA GTTCCATTGT ATAACGATGT AATGCTCCCC 
TCATCTAATG AGAAATTGCA GAATATAAAT TTGGCGATGT CTCCATTACC TGCCTCTGAA 
ACTCCAAAGC CACTTAGAAG TAGTGCTGAC CCTGCACTCA ACCAAGAAGT TGCATTAAAG 
TTAGAACCAA ATCCAGAGTC ACTGGAACTT TCTTTTACCA TGCCCCAGAT TCAAGATCAG 
CCAGCTAGTC CTTCTGATGG AAGCACCAGA CAAAGTTCAC CTGAGCCTAA CAGTCCCAGT 
GAATATTGTT TTGATGTGGA TAGTGATATG GTCAATGAAT TCAAGTTGGA ATTGGTAGAG 
AAACTTTTTG CTGAAGATAC AGAAGCAAAG AATCCATTTT CCACTCAGGA CACTGATTTA 
GACTTGGAGA TGTTAGCTCC TTATATCCCA ATGGATGATG ACTTCCAGTT ACGTTCCTTC 
GATCAGTTGT CACCACTGGA AAACAGTTCT ACAAGTCCTC AGAGTGCAAG CACAAATACA 
GTATTCCAGC CCACTCAAAT GCAAGAACCT CCCATAGCCA CCGTCACTAC CACAGCCACC 
AGTGATGAAT TGAAAACAGT GACAAAAGAT GGTATGGAAG ACATTAAAAT ACTGATTGCA 
TTTCCATCTC CTCCCCACGT ACCTAAAGAA CCTCCTTGTG CCACAACGTC ACCATATAGT 
GATACTGGAA GTCGGACAGC CTCACCCAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA 
GAAAAATCTC ATCCAAGAAG CCCTAATGTG TTATCTGTCG CTTTGAGTCA AAGAACTACT 
GCTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA 
AAAATTGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTGGAACATT ATTACAGCAA 
CCAGATGATC GTGCAACTAC CACATCACTT TCTTGGAAAC GTGTAAAAGG ATGCAAATCT 
AGTGAACAAA ATGGAATGGA GCAAAAGACA ATTATTTTAA TACCCTCTGA TTTAGCATGT 
AGACTGCTGG GGCAATCAAT GGATGAGAGT GGACTACCAC AGCTGACCAG TTATGATTGT 
GAAGTTAATG CTCCTATACA AGGCAGCAGA AACCTACTGC AGGGTGAAGA ATTACTCAGA 
GCTTTGGATC AAGTTAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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HIF1A cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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