NPHP1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	NPHP1
Entrez Gene ID	102392209
Full Name	nephrocystin 1
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006059827.1	XP_006059889.1	nephrocystin-1 isoform X1
XM_006059829.1	XP_006059891.1	nephrocystin-1 isoform X3
XM_006059828.1	XP_006059890.1	nephrocystin-1 isoform X2
XM_025260600.1	XP_025116385.1	LOW QUALITY PROTEIN: nephrocystin-1
XM_006059830.1	XP_006059892.1	nephrocystin-1 isoform X4
XM_006059831.1	XP_006059893.1	nephrocystin-1 isoform X5

Pathways from BioSystems
Gene Ontology

The following NPHP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPHP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu16364	XM_006059827.1 Latest version!	Bubalus bubalis nephronophthisis 1 (juvenile) (NPHP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu16365	XM_006059828.1 Latest version!	Bubalus bubalis nephronophthisis 1 (juvenile) (NPHP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu16366	XM_006059829.1 Latest version!	Bubalus bubalis nephronophthisis 1 (juvenile) (NPHP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu16367	XM_006059830.1 Latest version!	Bubalus bubalis nephronophthisis 1 (juvenile) (NPHP1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OBu16368	XM_006059831.1 Latest version!	Bubalus bubalis nephronophthisis 1 (juvenile) (NPHP1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OBu45685	XM_025260600.1 Latest version!	Bubalus bubalis nephrocystin 1 (NPHP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPHP1 ( XM_006059827.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006059889.1 Bubalus bubalis nephronophthisis 1 (juvenile) (NPHP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu16364
Clone ID Related Accession (Same CDS sequence)	XM_006059827.1
Accession Version	XM_006059827.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784941.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGATGTATC CAGTTAAAAC AGGCAATAGA TGAAAATAAA 
AATGCTCTTC AAAAATTAAA AAAAGCTGAT GAACCTGCAC CTGTGGGCAA TTATTATCAG 
AAGAAAGAAG AGGAGCACAA TCTTTTGGAT AAGCTTACCC ACCAACTGCA GGAGCTTGCT 
GTGTCCATAA GCAGAGAAAA TGTCACTGAA ACTGGGGCAC TTACTGAAAG AGAGGAAGAG 
GAAAACTCTG CTGAAGAAGA TGAGGAAGAA GAAGACGATG AAGAAGAAAG CGAAGAAAGT 
GGGGGCGAGG ACGAAGAAAC AGAAGAGGAA GAGGAAGAGA AACAGGAAGG TGAATCTCAG 
CCAGAAGCCA CGAGTGGAGA ATACATTGCT CTTGGAGATT TTATGGCTCA GCAAGTAGGA 
GACCTGACAT TTAAAAAAGG GGAAATTCTC TGTATAATCA AAAAAAACTC TGATGGTTGG 
TGGATGGCTA AGAACGCCAA AGGAAATGAA GGCCTTATTC CTAAAACCTA CTTGGAGCCC 
TATAATAAAG AAGGAGGTCA AGAATCCAGT GAAGAAGGCA GTGAAGAAGA TACAGAGGTA 
GGGGATGGAA CAGTGGAAGG AGCCGAAGTC AAGCAAAGAA CCGATTCTCA CTGGAGTGCT 
GTCCGGGAAG CCATCTCAGA GCAGATAAAC ACCGTCGACG TGCTGACCGC GATGGGAGCG 
ATTCCTGCTG GGTTCAGGCC TTCCACGCTC TTCCAGCTCC TGGAGGAAGG GAATCAATTC 
CGAGCAAGCT ACTTCTTACA GCCAGAGCTC ACACCTTCCC AACTGGCCTT CAGAGATCTG 
ATGTGGGATG CAAAAACCGG CTCTATCAGG TCAAGACCGA GCCGTGTCTC CTTGATTCTG 
ACTCTCTGGA GCTGCAAGAT GATTCCTCTT CCAGGGGTGA GCATCCAAGT TCTGAGCAGA 
CACGTGCGCC TCTGTCTGTT TGACGGCAGT GAGGTTCTGA GCAACATTCA CACAGTCAGG 
GCCACGTGGC AACCTAAGAA GCCCAAAACA TGGACCTTTT CTCCCCAGGT TACTGGCATC 
TTGCCCTGCC TGCTTGATGG CGACTGTTTC ATCAGATCCA ACTCTTCCTC TCCAGATCTT 
GGAATATTAT TTGAACTTGG AATTTCTTAT ATTCGCAATT CCACTGGTGA AAGAGGAGAG 
TTAAGCTGTG GCTGGGTGTT TCTGAAACTT TTTGACACCA CTGGAATTCC TATTCCAGCA 
AAGACTTACA AGCTCTTCTT GAACGGAGGC ACCCCTTATG AAAAAGGTGT TGAAGTGGAC 
CCTTCAGTAT CCACAAGAGC ACATGGTAGT GTTTTCCATC AGTTGATGAC AATGAGGAGG 
CAGCCTCAAC TTCTAGTCAA ACTGAGATCT CTGAACAGAC GATCCAGGAG CACGCTAAGT 
CTACTACCAG AAACATTAAT CGGAAGCATG TGCTGCGTTC ATTTGTTGAT ATTCTATCGC 
CAGATTCTTG GAGATGTTCT CCTGAAAGAC AGGATGAGCC TGAACAGTGC AGACTTAATT 
AGTAATCCAG TGCTGGCTAC CTTCCCCAAG CTCTTGGAGG AGTCGGACAT AATGGATGCA 
CTCAGGAGTT CCTGGGCTGA GAAAGAGAGT ACATTAAAAA GATCAGAGAA GGGAGACAGG 
GAGCTCCTGA AGGCGGTGTT CCTGCTGGTT TACCACGACT GTGCGCTCCC CCTGCTGCAC 
TCTGCGCTCC TGCCCCCGTC TAGATGGGCA GAGGAGGAGA CTGAAGCTGC CCGGTGGAAG 
GGCATCGCCG ACTTCCTCAA GCAAAACCAG GAAAATGAGG GCGCCCTCCA GGTTCTGCTG 
TCGCCAGAGG GGGTCCACGA ACCTTTCGAC ATTTCCGAGC AGACCTATGA CTTCTTGGGT 
GAAAGAAGAA AGAACGTAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006059889.1
CDS	65..2128
Translation	MLARRQRDPLQSLQRRNQELKQQVDGLLFESRLKGALEASKRRDMYQRCIQLKQAIDENKNALQKLKKADEPAPVGNYYQKKEEEHNLLDKLTHQLQELAVSISRENVTETGALTEREEEENSAEEDEEEEDDEEESEESGGEDEETEEEEEEKQEGESQPEATSGEYIALGDFMAQQVGDLTFKKGEILCIIKKNSDGWWMAKNAKGNEGLIPKTYLEPYNKEGGQESSEEGSEEDTEVGDGTVEGAEVKQRTDSHWSAVREAISEQINTVDVLTAMGAIPAGFRPSTLFQLLEEGNQFRASYFLQPELTPSQLAFRDLMWDAKTGSIRSRPSRVSLILTLWSCKMIPLPGVSIQVLSRHVRLCLFDGSEVLSNIHTVRATWQPKKPKTWTFSPQVTGILPCLLDGDCFIRSNSSSPDLGILFELGISYIRNSTGERGELSCGWVFLKLFDTTGIPIPAKTYKLFLNGGTPYEKGVEVDPSVSTRAHGSVFHQLMTMRRQPQLLVKLRSLNRRSRSTLSLLPETLIGSMCCVHLLIFYRQILGDVLLKDRMSLNSADLISNPVLATFPKLLEESDIMDALRSSWAEKESTLKRSEKGDRELLKAVFLLVYHDCALPLLHSALLPPSRWAEEETEAARWKGIADFLKQNQENEGALQVLLSPEGVHEPFDISEQTYDFLGERRKNVA

Target ORF information:

RefSeq Version	XM_006059827.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephronophthisis 1 (juvenile) (NPHP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006059827.1

ORF Insert Sequence:

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGATGTATC CAGTTAAAAC AGGCAATAGA TGAAAATAAA 
AATGCTCTTC AAAAATTAAA AAAAGCTGAT GAACCTGCAC CTGTGGGCAA TTATTATCAG 
AAGAAAGAAG AGGAGCACAA TCTTTTGGAT AAGCTTACCC ACCAACTGCA GGAGCTTGCT 
GTGTCCATAA GCAGAGAAAA TGTCACTGAA ACTGGGGCAC TTACTGAAAG AGAGGAAGAG 
GAAAACTCTG CTGAAGAAGA TGAGGAAGAA GAAGACGATG AAGAAGAAAG CGAAGAAAGT 
GGGGGCGAGG ACGAAGAAAC AGAAGAGGAA GAGGAAGAGA AACAGGAAGG TGAATCTCAG 
CCAGAAGCCA CGAGTGGAGA ATACATTGCT CTTGGAGATT TTATGGCTCA GCAAGTAGGA 
GACCTGACAT TTAAAAAAGG GGAAATTCTC TGTATAATCA AAAAAAACTC TGATGGTTGG 
TGGATGGCTA AGAACGCCAA AGGAAATGAA GGCCTTATTC CTAAAACCTA CTTGGAGCCC 
TATAATAAAG AAGGAGGTCA AGAATCCAGT GAAGAAGGCA GTGAAGAAGA TACAGAGGTA 
GGGGATGGAA CAGTGGAAGG AGCCGAAGTC AAGCAAAGAA CCGATTCTCA CTGGAGTGCT 
GTCCGGGAAG CCATCTCAGA GCAGATAAAC ACCGTCGACG TGCTGACCGC GATGGGAGCG 
ATTCCTGCTG GGTTCAGGCC TTCCACGCTC TTCCAGCTCC TGGAGGAAGG GAATCAATTC 
CGAGCAAGCT ACTTCTTACA GCCAGAGCTC ACACCTTCCC AACTGGCCTT CAGAGATCTG 
ATGTGGGATG CAAAAACCGG CTCTATCAGG TCAAGACCGA GCCGTGTCTC CTTGATTCTG 
ACTCTCTGGA GCTGCAAGAT GATTCCTCTT CCAGGGGTGA GCATCCAAGT TCTGAGCAGA 
CACGTGCGCC TCTGTCTGTT TGACGGCAGT GAGGTTCTGA GCAACATTCA CACAGTCAGG 
GCCACGTGGC AACCTAAGAA GCCCAAAACA TGGACCTTTT CTCCCCAGGT TACTGGCATC 
TTGCCCTGCC TGCTTGATGG CGACTGTTTC ATCAGATCCA ACTCTTCCTC TCCAGATCTT 
GGAATATTAT TTGAACTTGG AATTTCTTAT ATTCGCAATT CCACTGGTGA AAGAGGAGAG 
TTAAGCTGTG GCTGGGTGTT TCTGAAACTT TTTGACACCA CTGGAATTCC TATTCCAGCA 
AAGACTTACA AGCTCTTCTT GAACGGAGGC ACCCCTTATG AAAAAGGTGT TGAAGTGGAC 
CCTTCAGTAT CCACAAGAGC ACATGGTAGT GTTTTCCATC AGTTGATGAC AATGAGGAGG 
CAGCCTCAAC TTCTAGTCAA ACTGAGATCT CTGAACAGAC GATCCAGGAG CACGCTAAGT 
CTACTACCAG AAACATTAAT CGGAAGCATG TGCTGCGTTC ATTTGTTGAT ATTCTATCGC 
CAGATTCTTG GAGATGTTCT CCTGAAAGAC AGGATGAGCC TGAACAGTGC AGACTTAATT 
AGTAATCCAG TGCTGGCTAC CTTCCCCAAG CTCTTGGAGG AGTCGGACAT AATGGATGCA 
CTCAGGAGTT CCTGGGCTGA GAAAGAGAGT ACATTAAAAA GATCAGAGAA GGGAGACAGG 
GAGCTCCTGA AGGCGGTGTT CCTGCTGGTT TACCACGACT GTGCGCTCCC CCTGCTGCAC 
TCTGCGCTCC TGCCCCCGTC TAGATGGGCA GAGGAGGAGA CTGAAGCTGC CCGGTGGAAG 
GGCATCGCCG ACTTCCTCAA GCAAAACCAG GAAAATGAGG GCGCCCTCCA GGTTCTGCTG 
TCGCCAGAGG GGGTCCACGA ACCTTTCGAC ATTTCCGAGC AGACCTATGA CTTCTTGGGT 
GAAAGAAGAA AGAACGTAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP1 ( XM_006059828.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006059890.1 Bubalus bubalis nephronophthisis 1 (juvenile) (NPHP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu16365
Clone ID Related Accession (Same CDS sequence)	XM_006059828.1
Accession Version	XM_006059828.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784941.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGATGTATC CAGTTAAAAC AGGCAATAGA TGAAAATAAA 
AATGCTCTTC AAAAATTAAA AAAAGCTGAT GAACCTGCAC CTGTGGGCAA TTATTATCAG 
AAGAAAGAAG AGGAGCACAA TCTTTTGGAT AAGCTTACCC ACCAACTGCA GGAGCTTGCT 
GTGTCCATAA GCAGAGAAAA TGTCACTGAA ACTGGGGCAC TTACTGAAAG AGAGGAAGAG 
GAAAACTCTG CTGAAGAAGA TGAGGAAGAA GAAGACGATG AAGAAGAAAG CGAAGAAAGT 
GGGGGCGAGG ACGAAGAAAC AGAAGAGGAA GAGGAAGAGA AACAGGAAGG TGAATCTCAG 
CCAGAAGCCA CGAGTGGAGA ATACATTGCT CTTGGAGATT TTATGGCTCA GCAAGTAGGA 
GACCTGACAT TTAAAAAAGG GGAAATTCTC TGTATAATCA AAAAAAACTC TGATGGTTGG 
TGGATGGCTA AGAACGCCAA AGGAAATGAA GGCCTTATTC CTAAAACCTA CTTGGAGCCC 
TATAATAAAG AAGGAGGTCA AGAATCCAGT GAAGAAGGCA GTGAAGAAGA TACAGAGGTA 
GGGGATGGAA CAGTGGAAGG AGCCGAAGTC AAGCAAAGAA CCGATTCTCA CTGGAGTGCT 
GTCCGGGAAG CCATCTCAGA GATAAACACC GTCGACGTGC TGACCGCGAT GGGAGCGATT 
CCTGCTGGGT TCAGGCCTTC CACGCTCTTC CAGCTCCTGG AGGAAGGGAA TCAATTCCGA 
GCAAGCTACT TCTTACAGCC AGAGCTCACA CCTTCCCAAC TGGCCTTCAG AGATCTGATG 
TGGGATGCAA AAACCGGCTC TATCAGGTCA AGACCGAGCC GTGTCTCCTT GATTCTGACT 
CTCTGGAGCT GCAAGATGAT TCCTCTTCCA GGGGTGAGCA TCCAAGTTCT GAGCAGACAC 
GTGCGCCTCT GTCTGTTTGA CGGCAGTGAG GTTCTGAGCA ACATTCACAC AGTCAGGGCC 
ACGTGGCAAC CTAAGAAGCC CAAAACATGG ACCTTTTCTC CCCAGGTTAC TGGCATCTTG 
CCCTGCCTGC TTGATGGCGA CTGTTTCATC AGATCCAACT CTTCCTCTCC AGATCTTGGA 
ATATTATTTG AACTTGGAAT TTCTTATATT CGCAATTCCA CTGGTGAAAG AGGAGAGTTA 
AGCTGTGGCT GGGTGTTTCT GAAACTTTTT GACACCACTG GAATTCCTAT TCCAGCAAAG 
ACTTACAAGC TCTTCTTGAA CGGAGGCACC CCTTATGAAA AAGGTGTTGA AGTGGACCCT 
TCAGTATCCA CAAGAGCACA TGGTAGTGTT TTCCATCAGT TGATGACAAT GAGGAGGCAG 
CCTCAACTTC TAGTCAAACT GAGATCTCTG AACAGACGAT CCAGGAGCAC GCTAAGTCTA 
CTACCAGAAA CATTAATCGG AAGCATGTGC TGCGTTCATT TGTTGATATT CTATCGCCAG 
ATTCTTGGAG ATGTTCTCCT GAAAGACAGG ATGAGCCTGA ACAGTGCAGA CTTAATTAGT 
AATCCAGTGC TGGCTACCTT CCCCAAGCTC TTGGAGGAGT CGGACATAAT GGATGCACTC 
AGGAGTTCCT GGGCTGAGAA AGAGAGTACA TTAAAAAGAT CAGAGAAGGG AGACAGGGAG 
CTCCTGAAGG CGGTGTTCCT GCTGGTTTAC CACGACTGTG CGCTCCCCCT GCTGCACTCT 
GCGCTCCTGC CCCCGTCTAG ATGGGCAGAG GAGGAGACTG AAGCTGCCCG GTGGAAGGGC 
ATCGCCGACT TCCTCAAGCA AAACCAGGAA AATGAGGGCG CCCTCCAGGT TCTGCTGTCG 
CCAGAGGGGG TCCACGAACC TTTCGACATT TCCGAGCAGA CCTATGACTT CTTGGGTGAA 
AGAAGAAAGA ACGTAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006059890.1
CDS	65..2125
Translation	MLARRQRDPLQSLQRRNQELKQQVDGLLFESRLKGALEASKRRDMYQRCIQLKQAIDENKNALQKLKKADEPAPVGNYYQKKEEEHNLLDKLTHQLQELAVSISRENVTETGALTEREEEENSAEEDEEEEDDEEESEESGGEDEETEEEEEEKQEGESQPEATSGEYIALGDFMAQQVGDLTFKKGEILCIIKKNSDGWWMAKNAKGNEGLIPKTYLEPYNKEGGQESSEEGSEEDTEVGDGTVEGAEVKQRTDSHWSAVREAISEINTVDVLTAMGAIPAGFRPSTLFQLLEEGNQFRASYFLQPELTPSQLAFRDLMWDAKTGSIRSRPSRVSLILTLWSCKMIPLPGVSIQVLSRHVRLCLFDGSEVLSNIHTVRATWQPKKPKTWTFSPQVTGILPCLLDGDCFIRSNSSSPDLGILFELGISYIRNSTGERGELSCGWVFLKLFDTTGIPIPAKTYKLFLNGGTPYEKGVEVDPSVSTRAHGSVFHQLMTMRRQPQLLVKLRSLNRRSRSTLSLLPETLIGSMCCVHLLIFYRQILGDVLLKDRMSLNSADLISNPVLATFPKLLEESDIMDALRSSWAEKESTLKRSEKGDRELLKAVFLLVYHDCALPLLHSALLPPSRWAEEETEAARWKGIADFLKQNQENEGALQVLLSPEGVHEPFDISEQTYDFLGERRKNVA

Target ORF information:

RefSeq Version	XM_006059828.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephronophthisis 1 (juvenile) (NPHP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006059828.1

ORF Insert Sequence:

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGATGTATC CAGTTAAAAC AGGCAATAGA TGAAAATAAA 
AATGCTCTTC AAAAATTAAA AAAAGCTGAT GAACCTGCAC CTGTGGGCAA TTATTATCAG 
AAGAAAGAAG AGGAGCACAA TCTTTTGGAT AAGCTTACCC ACCAACTGCA GGAGCTTGCT 
GTGTCCATAA GCAGAGAAAA TGTCACTGAA ACTGGGGCAC TTACTGAAAG AGAGGAAGAG 
GAAAACTCTG CTGAAGAAGA TGAGGAAGAA GAAGACGATG AAGAAGAAAG CGAAGAAAGT 
GGGGGCGAGG ACGAAGAAAC AGAAGAGGAA GAGGAAGAGA AACAGGAAGG TGAATCTCAG 
CCAGAAGCCA CGAGTGGAGA ATACATTGCT CTTGGAGATT TTATGGCTCA GCAAGTAGGA 
GACCTGACAT TTAAAAAAGG GGAAATTCTC TGTATAATCA AAAAAAACTC TGATGGTTGG 
TGGATGGCTA AGAACGCCAA AGGAAATGAA GGCCTTATTC CTAAAACCTA CTTGGAGCCC 
TATAATAAAG AAGGAGGTCA AGAATCCAGT GAAGAAGGCA GTGAAGAAGA TACAGAGGTA 
GGGGATGGAA CAGTGGAAGG AGCCGAAGTC AAGCAAAGAA CCGATTCTCA CTGGAGTGCT 
GTCCGGGAAG CCATCTCAGA GATAAACACC GTCGACGTGC TGACCGCGAT GGGAGCGATT 
CCTGCTGGGT TCAGGCCTTC CACGCTCTTC CAGCTCCTGG AGGAAGGGAA TCAATTCCGA 
GCAAGCTACT TCTTACAGCC AGAGCTCACA CCTTCCCAAC TGGCCTTCAG AGATCTGATG 
TGGGATGCAA AAACCGGCTC TATCAGGTCA AGACCGAGCC GTGTCTCCTT GATTCTGACT 
CTCTGGAGCT GCAAGATGAT TCCTCTTCCA GGGGTGAGCA TCCAAGTTCT GAGCAGACAC 
GTGCGCCTCT GTCTGTTTGA CGGCAGTGAG GTTCTGAGCA ACATTCACAC AGTCAGGGCC 
ACGTGGCAAC CTAAGAAGCC CAAAACATGG ACCTTTTCTC CCCAGGTTAC TGGCATCTTG 
CCCTGCCTGC TTGATGGCGA CTGTTTCATC AGATCCAACT CTTCCTCTCC AGATCTTGGA 
ATATTATTTG AACTTGGAAT TTCTTATATT CGCAATTCCA CTGGTGAAAG AGGAGAGTTA 
AGCTGTGGCT GGGTGTTTCT GAAACTTTTT GACACCACTG GAATTCCTAT TCCAGCAAAG 
ACTTACAAGC TCTTCTTGAA CGGAGGCACC CCTTATGAAA AAGGTGTTGA AGTGGACCCT 
TCAGTATCCA CAAGAGCACA TGGTAGTGTT TTCCATCAGT TGATGACAAT GAGGAGGCAG 
CCTCAACTTC TAGTCAAACT GAGATCTCTG AACAGACGAT CCAGGAGCAC GCTAAGTCTA 
CTACCAGAAA CATTAATCGG AAGCATGTGC TGCGTTCATT TGTTGATATT CTATCGCCAG 
ATTCTTGGAG ATGTTCTCCT GAAAGACAGG ATGAGCCTGA ACAGTGCAGA CTTAATTAGT 
AATCCAGTGC TGGCTACCTT CCCCAAGCTC TTGGAGGAGT CGGACATAAT GGATGCACTC 
AGGAGTTCCT GGGCTGAGAA AGAGAGTACA TTAAAAAGAT CAGAGAAGGG AGACAGGGAG 
CTCCTGAAGG CGGTGTTCCT GCTGGTTTAC CACGACTGTG CGCTCCCCCT GCTGCACTCT 
GCGCTCCTGC CCCCGTCTAG ATGGGCAGAG GAGGAGACTG AAGCTGCCCG GTGGAAGGGC 
ATCGCCGACT TCCTCAAGCA AAACCAGGAA AATGAGGGCG CCCTCCAGGT TCTGCTGTCG 
CCAGAGGGGG TCCACGAACC TTTCGACATT TCCGAGCAGA CCTATGACTT CTTGGGTGAA 
AGAAGAAAGA ACGTAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP1 ( XM_006059829.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006059891.1 Bubalus bubalis nephronophthisis 1 (juvenile) (NPHP1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu16366
Clone ID Related Accession (Same CDS sequence)	XM_006059829.1
Accession Version	XM_006059829.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784941.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGATGTATC CAGTTAAAAC AGGCAATAGA TGAAAATAAA 
AATGCTCTTC AAAAATTAAA AAAAGCTGAT GAACCTGCAC CTGTGGGCAA TTATTATCAG 
AAGAAAGAAG AGGAGCACAA TCTTTTGGAT AAGCTTACCC ACCAACTGCA GGAGCTTGCT 
GTGTCCATAA GCAGAGAAAA TGTCACTGAA ACTGGGGCAC TTACTGAAAG AGAGGAAGAG 
GAAAACTCTG CTGAAGAAGA TGAGGAAGAA GAAGACGATG AAGAAGAAAG CGAAGAAAGT 
GGGGGCGAGG ACGAAGAAAC AGAAGAGGAA GAGGAAGAGA AACAGGAAGG TGAATCTCAG 
CCAGAAGCCA CGAGTGGAGA ATACATTGCT CTTGGAGATT TTATGGCTCA GCAAGTAGGA 
GACCTGACAT TTAAAAAAGG GGAAATTCTC TGTATAATCA AAAAAAACTC TGATGGTTGG 
TGGATGGCTA AGAACGCCAA AGGAAATGAA GGCCTTATTC CTAAAACCTA CTTGGAGCCC 
TATAATAAAG AAGGAGGTCA AGAATCCAGT GAAGAAGGCA GTGAAGAAGA TACAGAGGTA 
GGGGATGGAA CAGTGGAAGG AGCCGAAGTC AAGCAAAGAA CCGATTCTCA CTGGAGTGCT 
GTCCGGGAAG CCATCTCAGA GCAGATAAAC ACCGTCGACG TGCTGACCGC GATGGGAGCG 
ATTCCTGCTG GGTTCAGGCC TTCCACGCTC TTCCAGCTCC TGGAGGAAGG GAATCAATTC 
CGAGCAAGCT ACTTCTTACA GCCAGAGCTC ACACCTTCCC AACTGGCCTT CAGAGATCTG 
ATGTGGGATG CAAAAACCGG CTCTATCAGG TCAAGACCGA GCCGTGTCTC CTTGATTCTG 
ACTCTCTGGA GCTGCAAGAT GATTCCTCTT CCAGGGGTTC TGAGCAACAT TCACACAGTC 
AGGGCCACGT GGCAACCTAA GAAGCCCAAA ACATGGACCT TTTCTCCCCA GGTTACTGGC 
ATCTTGCCCT GCCTGCTTGA TGGCGACTGT TTCATCAGAT CCAACTCTTC CTCTCCAGAT 
CTTGGAATAT TATTTGAACT TGGAATTTCT TATATTCGCA ATTCCACTGG TGAAAGAGGA 
GAGTTAAGCT GTGGCTGGGT GTTTCTGAAA CTTTTTGACA CCACTGGAAT TCCTATTCCA 
GCAAAGACTT ACAAGCTCTT CTTGAACGGA GGCACCCCTT ATGAAAAAGG TGTTGAAGTG 
GACCCTTCAG TATCCACAAG AGCACATGGT AGTGTTTTCC ATCAGTTGAT GACAATGAGG 
AGGCAGCCTC AACTTCTAGT CAAACTGAGA TCTCTGAACA GACGATCCAG GAGCACGCTA 
AGTCTACTAC CAGAAACATT AATCGGAAGC ATGTGCTGCG TTCATTTGTT GATATTCTAT 
CGCCAGATTC TTGGAGATGT TCTCCTGAAA GACAGGATGA GCCTGAACAG TGCAGACTTA 
ATTAGTAATC CAGTGCTGGC TACCTTCCCC AAGCTCTTGG AGGAGTCGGA CATAATGGAT 
GCACTCAGGA GTTCCTGGGC TGAGAAAGAG AGTACATTAA AAAGATCAGA GAAGGGAGAC 
AGGGAGCTCC TGAAGGCGGT GTTCCTGCTG GTTTACCACG ACTGTGCGCT CCCCCTGCTG 
CACTCTGCGC TCCTGCCCCC GTCTAGATGG GCAGAGGAGG AGACTGAAGC TGCCCGGTGG 
AAGGGCATCG CCGACTTCCT CAAGCAAAAC CAGGAAAATG AGGGCGCCCT CCAGGTTCTG 
CTGTCGCCAG AGGGGGTCCA CGAACCTTTC GACATTTCCG AGCAGACCTA TGACTTCTTG 
GGTGAAAGAA GAAAGAACGT AGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006059891.1
CDS	65..2071
Translation	MLARRQRDPLQSLQRRNQELKQQVDGLLFESRLKGALEASKRRDMYQRCIQLKQAIDENKNALQKLKKADEPAPVGNYYQKKEEEHNLLDKLTHQLQELAVSISRENVTETGALTEREEEENSAEEDEEEEDDEEESEESGGEDEETEEEEEEKQEGESQPEATSGEYIALGDFMAQQVGDLTFKKGEILCIIKKNSDGWWMAKNAKGNEGLIPKTYLEPYNKEGGQESSEEGSEEDTEVGDGTVEGAEVKQRTDSHWSAVREAISEQINTVDVLTAMGAIPAGFRPSTLFQLLEEGNQFRASYFLQPELTPSQLAFRDLMWDAKTGSIRSRPSRVSLILTLWSCKMIPLPGVLSNIHTVRATWQPKKPKTWTFSPQVTGILPCLLDGDCFIRSNSSSPDLGILFELGISYIRNSTGERGELSCGWVFLKLFDTTGIPIPAKTYKLFLNGGTPYEKGVEVDPSVSTRAHGSVFHQLMTMRRQPQLLVKLRSLNRRSRSTLSLLPETLIGSMCCVHLLIFYRQILGDVLLKDRMSLNSADLISNPVLATFPKLLEESDIMDALRSSWAEKESTLKRSEKGDRELLKAVFLLVYHDCALPLLHSALLPPSRWAEEETEAARWKGIADFLKQNQENEGALQVLLSPEGVHEPFDISEQTYDFLGERRKNVA

Target ORF information:

RefSeq Version	XM_006059829.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephronophthisis 1 (juvenile) (NPHP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006059829.1

ORF Insert Sequence:

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGATGTATC CAGTTAAAAC AGGCAATAGA TGAAAATAAA 
AATGCTCTTC AAAAATTAAA AAAAGCTGAT GAACCTGCAC CTGTGGGCAA TTATTATCAG 
AAGAAAGAAG AGGAGCACAA TCTTTTGGAT AAGCTTACCC ACCAACTGCA GGAGCTTGCT 
GTGTCCATAA GCAGAGAAAA TGTCACTGAA ACTGGGGCAC TTACTGAAAG AGAGGAAGAG 
GAAAACTCTG CTGAAGAAGA TGAGGAAGAA GAAGACGATG AAGAAGAAAG CGAAGAAAGT 
GGGGGCGAGG ACGAAGAAAC AGAAGAGGAA GAGGAAGAGA AACAGGAAGG TGAATCTCAG 
CCAGAAGCCA CGAGTGGAGA ATACATTGCT CTTGGAGATT TTATGGCTCA GCAAGTAGGA 
GACCTGACAT TTAAAAAAGG GGAAATTCTC TGTATAATCA AAAAAAACTC TGATGGTTGG 
TGGATGGCTA AGAACGCCAA AGGAAATGAA GGCCTTATTC CTAAAACCTA CTTGGAGCCC 
TATAATAAAG AAGGAGGTCA AGAATCCAGT GAAGAAGGCA GTGAAGAAGA TACAGAGGTA 
GGGGATGGAA CAGTGGAAGG AGCCGAAGTC AAGCAAAGAA CCGATTCTCA CTGGAGTGCT 
GTCCGGGAAG CCATCTCAGA GCAGATAAAC ACCGTCGACG TGCTGACCGC GATGGGAGCG 
ATTCCTGCTG GGTTCAGGCC TTCCACGCTC TTCCAGCTCC TGGAGGAAGG GAATCAATTC 
CGAGCAAGCT ACTTCTTACA GCCAGAGCTC ACACCTTCCC AACTGGCCTT CAGAGATCTG 
ATGTGGGATG CAAAAACCGG CTCTATCAGG TCAAGACCGA GCCGTGTCTC CTTGATTCTG 
ACTCTCTGGA GCTGCAAGAT GATTCCTCTT CCAGGGGTTC TGAGCAACAT TCACACAGTC 
AGGGCCACGT GGCAACCTAA GAAGCCCAAA ACATGGACCT TTTCTCCCCA GGTTACTGGC 
ATCTTGCCCT GCCTGCTTGA TGGCGACTGT TTCATCAGAT CCAACTCTTC CTCTCCAGAT 
CTTGGAATAT TATTTGAACT TGGAATTTCT TATATTCGCA ATTCCACTGG TGAAAGAGGA 
GAGTTAAGCT GTGGCTGGGT GTTTCTGAAA CTTTTTGACA CCACTGGAAT TCCTATTCCA 
GCAAAGACTT ACAAGCTCTT CTTGAACGGA GGCACCCCTT ATGAAAAAGG TGTTGAAGTG 
GACCCTTCAG TATCCACAAG AGCACATGGT AGTGTTTTCC ATCAGTTGAT GACAATGAGG 
AGGCAGCCTC AACTTCTAGT CAAACTGAGA TCTCTGAACA GACGATCCAG GAGCACGCTA 
AGTCTACTAC CAGAAACATT AATCGGAAGC ATGTGCTGCG TTCATTTGTT GATATTCTAT 
CGCCAGATTC TTGGAGATGT TCTCCTGAAA GACAGGATGA GCCTGAACAG TGCAGACTTA 
ATTAGTAATC CAGTGCTGGC TACCTTCCCC AAGCTCTTGG AGGAGTCGGA CATAATGGAT 
GCACTCAGGA GTTCCTGGGC TGAGAAAGAG AGTACATTAA AAAGATCAGA GAAGGGAGAC 
AGGGAGCTCC TGAAGGCGGT GTTCCTGCTG GTTTACCACG ACTGTGCGCT CCCCCTGCTG 
CACTCTGCGC TCCTGCCCCC GTCTAGATGG GCAGAGGAGG AGACTGAAGC TGCCCGGTGG 
AAGGGCATCG CCGACTTCCT CAAGCAAAAC CAGGAAAATG AGGGCGCCCT CCAGGTTCTG 
CTGTCGCCAG AGGGGGTCCA CGAACCTTTC GACATTTCCG AGCAGACCTA TGACTTCTTG 
GGTGAAAGAA GAAAGAACGT AGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP1 ( XM_006059830.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006059892.1 Bubalus bubalis nephronophthisis 1 (juvenile) (NPHP1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu16367
Clone ID Related Accession (Same CDS sequence)	XM_006059830.1
Accession Version	XM_006059830.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784941.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCAAA GATGTATCCA GTTAAAACAG GCAATAGATG AAAATAAAAA TGCTCTTCAA 
AAATTAAAAA AAGCTGATGA ACCTGCACCT GTGGGCAATT ATTATCAGAA GAAAGAAGAG 
GAGCACAATC TTTTGGATAA GCTTACCCAC CAACTGCAGG AGCTTGCTGT GTCCATAAGC 
AGAGAAAATG TCACTGAAAC TGGGGCACTT ACTGAAAGAG AGGAAGAGGA AAACTCTGCT 
GAAGAAGATG AGGAAGAAGA AGACGATGAA GAAGAAAGCG AAGAAAGTGG GGGCGAGGAC 
GAAGAAACAG AAGAGGAAGA GGAAGAGAAA CAGGAAGGTG AATCTCAGCC AGAAGCCACG 
AGTGGAGAAT ACATTGCTCT TGGAGATTTT ATGGCTCAGC AAGTAGGAGA CCTGACATTT 
AAAAAAGGGG AAATTCTCTG TATAATCAAA AAAAACTCTG ATGGTTGGTG GATGGCTAAG 
AACGCCAAAG GAAATGAAGG CCTTATTCCT AAAACCTACT TGGAGCCCTA TAATAAAGAA 
GGAGGTCAAG AATCCAGTGA AGAAGGCAGT GAAGAAGATA CAGAGGTAGG GGATGGAACA 
GTGGAAGGAG CCGAAGTCAA GCAAAGAACC GATTCTCACT GGAGTGCTGT CCGGGAAGCC 
ATCTCAGAGC AGATAAACAC CGTCGACGTG CTGACCGCGA TGGGAGCGAT TCCTGCTGGG 
TTCAGGCCTT CCACGCTCTT CCAGCTCCTG GAGGAAGGGA ATCAATTCCG AGCAAGCTAC 
TTCTTACAGC CAGAGCTCAC ACCTTCCCAA CTGGCCTTCA GAGATCTGAT GTGGGATGCA 
AAAACCGGCT CTATCAGGTC AAGACCGAGC CGTGTCTCCT TGATTCTGAC TCTCTGGAGC 
TGCAAGATGA TTCCTCTTCC AGGGGTGAGC ATCCAAGTTC TGAGCAGACA CGTGCGCCTC 
TGTCTGTTTG ACGGCAGTGA GGTTCTGAGC AACATTCACA CAGTCAGGGC CACGTGGCAA 
CCTAAGAAGC CCAAAACATG GACCTTTTCT CCCCAGGTTA CTGGCATCTT GCCCTGCCTG 
CTTGATGGCG ACTGTTTCAT CAGATCCAAC TCTTCCTCTC CAGATCTTGG AATATTATTT 
GAACTTGGAA TTTCTTATAT TCGCAATTCC ACTGGTGAAA GAGGAGAGTT AAGCTGTGGC 
TGGGTGTTTC TGAAACTTTT TGACACCACT GGAATTCCTA TTCCAGCAAA GACTTACAAG 
CTCTTCTTGA ACGGAGGCAC CCCTTATGAA AAAGGTGTTG AAGTGGACCC TTCAGTATCC 
ACAAGAGCAC ATGGTAGTGT TTTCCATCAG TTGATGACAA TGAGGAGGCA GCCTCAACTT 
CTAGTCAAAC TGAGATCTCT GAACAGACGA TCCAGGAGCA CGCTAAGTCT ACTACCAGAA 
ACATTAATCG GAAGCATGTG CTGCGTTCAT TTGTTGATAT TCTATCGCCA GATTCTTGGA 
GATGTTCTCC TGAAAGACAG GATGAGCCTG AACAGTGCAG ACTTAATTAG TAATCCAGTG 
CTGGCTACCT TCCCCAAGCT CTTGGAGGAG TCGGACATAA TGGATGCACT CAGGAGTTCC 
TGGGCTGAGA AAGAGAGTAC ATTAAAAAGA TCAGAGAAGG GAGACAGGGA GCTCCTGAAG 
GCGGTGTTCC TGCTGGTTTA CCACGACTGT GCGCTCCCCC TGCTGCACTC TGCGCTCCTG 
CCCCCGTCTA GATGGGCAGA GGAGGAGACT GAAGCTGCCC GGTGGAAGGG CATCGCCGAC 
TTCCTCAAGC AAAACCAGGA AAATGAGGGC GCCCTCCAGG TTCTGCTGTC GCCAGAGGGG 
GTCCACGAAC CTTTCGACAT TTCCGAGCAG ACCTATGACT TCTTGGGTGA AAGAAGAAAG 
AACGTAGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006059892.1
CDS	113..2044
Translation	MYQRCIQLKQAIDENKNALQKLKKADEPAPVGNYYQKKEEEHNLLDKLTHQLQELAVSISRENVTETGALTEREEEENSAEEDEEEEDDEEESEESGGEDEETEEEEEEKQEGESQPEATSGEYIALGDFMAQQVGDLTFKKGEILCIIKKNSDGWWMAKNAKGNEGLIPKTYLEPYNKEGGQESSEEGSEEDTEVGDGTVEGAEVKQRTDSHWSAVREAISEQINTVDVLTAMGAIPAGFRPSTLFQLLEEGNQFRASYFLQPELTPSQLAFRDLMWDAKTGSIRSRPSRVSLILTLWSCKMIPLPGVSIQVLSRHVRLCLFDGSEVLSNIHTVRATWQPKKPKTWTFSPQVTGILPCLLDGDCFIRSNSSSPDLGILFELGISYIRNSTGERGELSCGWVFLKLFDTTGIPIPAKTYKLFLNGGTPYEKGVEVDPSVSTRAHGSVFHQLMTMRRQPQLLVKLRSLNRRSRSTLSLLPETLIGSMCCVHLLIFYRQILGDVLLKDRMSLNSADLISNPVLATFPKLLEESDIMDALRSSWAEKESTLKRSEKGDRELLKAVFLLVYHDCALPLLHSALLPPSRWAEEETEAARWKGIADFLKQNQENEGALQVLLSPEGVHEPFDISEQTYDFLGERRKNVA

Target ORF information:

RefSeq Version	XM_006059830.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephronophthisis 1 (juvenile) (NPHP1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006059830.1

ORF Insert Sequence:

ATGTACCAAA GATGTATCCA GTTAAAACAG GCAATAGATG AAAATAAAAA TGCTCTTCAA 
AAATTAAAAA AAGCTGATGA ACCTGCACCT GTGGGCAATT ATTATCAGAA GAAAGAAGAG 
GAGCACAATC TTTTGGATAA GCTTACCCAC CAACTGCAGG AGCTTGCTGT GTCCATAAGC 
AGAGAAAATG TCACTGAAAC TGGGGCACTT ACTGAAAGAG AGGAAGAGGA AAACTCTGCT 
GAAGAAGATG AGGAAGAAGA AGACGATGAA GAAGAAAGCG AAGAAAGTGG GGGCGAGGAC 
GAAGAAACAG AAGAGGAAGA GGAAGAGAAA CAGGAAGGTG AATCTCAGCC AGAAGCCACG 
AGTGGAGAAT ACATTGCTCT TGGAGATTTT ATGGCTCAGC AAGTAGGAGA CCTGACATTT 
AAAAAAGGGG AAATTCTCTG TATAATCAAA AAAAACTCTG ATGGTTGGTG GATGGCTAAG 
AACGCCAAAG GAAATGAAGG CCTTATTCCT AAAACCTACT TGGAGCCCTA TAATAAAGAA 
GGAGGTCAAG AATCCAGTGA AGAAGGCAGT GAAGAAGATA CAGAGGTAGG GGATGGAACA 
GTGGAAGGAG CCGAAGTCAA GCAAAGAACC GATTCTCACT GGAGTGCTGT CCGGGAAGCC 
ATCTCAGAGC AGATAAACAC CGTCGACGTG CTGACCGCGA TGGGAGCGAT TCCTGCTGGG 
TTCAGGCCTT CCACGCTCTT CCAGCTCCTG GAGGAAGGGA ATCAATTCCG AGCAAGCTAC 
TTCTTACAGC CAGAGCTCAC ACCTTCCCAA CTGGCCTTCA GAGATCTGAT GTGGGATGCA 
AAAACCGGCT CTATCAGGTC AAGACCGAGC CGTGTCTCCT TGATTCTGAC TCTCTGGAGC 
TGCAAGATGA TTCCTCTTCC AGGGGTGAGC ATCCAAGTTC TGAGCAGACA CGTGCGCCTC 
TGTCTGTTTG ACGGCAGTGA GGTTCTGAGC AACATTCACA CAGTCAGGGC CACGTGGCAA 
CCTAAGAAGC CCAAAACATG GACCTTTTCT CCCCAGGTTA CTGGCATCTT GCCCTGCCTG 
CTTGATGGCG ACTGTTTCAT CAGATCCAAC TCTTCCTCTC CAGATCTTGG AATATTATTT 
GAACTTGGAA TTTCTTATAT TCGCAATTCC ACTGGTGAAA GAGGAGAGTT AAGCTGTGGC 
TGGGTGTTTC TGAAACTTTT TGACACCACT GGAATTCCTA TTCCAGCAAA GACTTACAAG 
CTCTTCTTGA ACGGAGGCAC CCCTTATGAA AAAGGTGTTG AAGTGGACCC TTCAGTATCC 
ACAAGAGCAC ATGGTAGTGT TTTCCATCAG TTGATGACAA TGAGGAGGCA GCCTCAACTT 
CTAGTCAAAC TGAGATCTCT GAACAGACGA TCCAGGAGCA CGCTAAGTCT ACTACCAGAA 
ACATTAATCG GAAGCATGTG CTGCGTTCAT TTGTTGATAT TCTATCGCCA GATTCTTGGA 
GATGTTCTCC TGAAAGACAG GATGAGCCTG AACAGTGCAG ACTTAATTAG TAATCCAGTG 
CTGGCTACCT TCCCCAAGCT CTTGGAGGAG TCGGACATAA TGGATGCACT CAGGAGTTCC 
TGGGCTGAGA AAGAGAGTAC ATTAAAAAGA TCAGAGAAGG GAGACAGGGA GCTCCTGAAG 
GCGGTGTTCC TGCTGGTTTA CCACGACTGT GCGCTCCCCC TGCTGCACTC TGCGCTCCTG 
CCCCCGTCTA GATGGGCAGA GGAGGAGACT GAAGCTGCCC GGTGGAAGGG CATCGCCGAC 
TTCCTCAAGC AAAACCAGGA AAATGAGGGC GCCCTCCAGG TTCTGCTGTC GCCAGAGGGG 
GTCCACGAAC CTTTCGACAT TTCCGAGCAG ACCTATGACT TCTTGGGTGA AAGAAGAAAG 
AACGTAGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP1 ( XM_006059831.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006059893.1 Bubalus bubalis nephronophthisis 1 (juvenile) (NPHP1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu16368
Clone ID Related Accession (Same CDS sequence)	XM_006059831.1
Accession Version	XM_006059831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	nephrocystin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784941.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGAACTGGG GCACTTACTG AAAGAGAGGA AGAGGAAAAC 
TCTGCTGAAG AAGATGAGGA AGAAGAAGAC GATGAAGAAG AAAGCGAAGA AAGTGGGGGC 
GAGGACGAAG AAACAGAAGA GGAAGAGGAA GAGAAACAGG AAGGTGAATC TCAGCCAGAA 
GCCACGAGTG GAGAATACAT TGCTCTTGGA GATTTTATGG CTCAGCAAGT AGGAGACCTG 
ACATTTAAAA AAGGGGAAAT TCTCTGTATA ATCAAAAAAA ACTCTGATGG TTGGTGGATG 
GCTAAGAACG CCAAAGGAAA TGAAGGCCTT ATTCCTAAAA CCTACTTGGA GCCCTATAAT 
AAAGAAGGAG GTCAAGAATC CAGTGAAGAA GGCAGTGAAG AAGATACAGA GGTAGGGGAT 
GGAACAGTGG AAGGAGCCGA AGTCAAGCAA AGAACCGATT CTCACTGGAG TGCTGTCCGG 
GAAGCCATCT CAGAGATAAA CACCGTCGAC GTGCTGACCG CGATGGGAGC GATTCCTGCT 
GGGTTCAGGC CTTCCACGCT CTTCCAGCTC CTGGAGGAAG GGAATCAATT CCGAGCAAGC 
TACTTCTTAC AGCCAGAGCT CACACCTTCC CAACTGGCCT TCAGAGATCT GATGTGGGAT 
GCAAAAACCG GCTCTATCAG GTCAAGACCG AGCCGTGTCT CCTTGATTCT GACTCTCTGG 
AGCTGCAAGA TGATTCCTCT TCCAGGGGTG AGCATCCAAG TTCTGAGCAG ACACGTGCGC 
CTCTGTCTGT TTGACGGCAG TGAGGTTCTG AGCAACATTC ACACAGTCAG GGCCACGTGG 
CAACCTAAGA AGCCCAAAAC ATGGACCTTT TCTCCCCAGG TTACTGGCAT CTTGCCCTGC 
CTGCTTGATG GCGACTGTTT CATCAGATCC AACTCTTCCT CTCCAGATCT TGGAATATTA 
TTTGAACTTG GAATTTCTTA TATTCGCAAT TCCACTGGTG AAAGAGGAGA GTTAAGCTGT 
GGCTGGGTGT TTCTGAAACT TTTTGACACC ACTGGAATTC CTATTCCAGC AAAGACTTAC 
AAGCTCTTCT TGAACGGAGG CACCCCTTAT GAAAAAGGTG TTGAAGTGGA CCCTTCAGTA 
TCCACAAGAG CACATGGTAG TGTTTTCCAT CAGTTGATGA CAATGAGGAG GCAGCCTCAA 
CTTCTAGTCA AACTGAGATC TCTGAACAGA CGATCCAGGA GCACGCTAAG TCTACTACCA 
GAAACATTAA TCGGAAGCAT GTGCTGCGTT CATTTGTTGA TATTCTATCG CCAGATTCTT 
GGAGATGTTC TCCTGAAAGA CAGGATGAGC CTGAACAGTG CAGACTTAAT TAGTAATCCA 
GTGCTGGCTA CCTTCCCCAA GCTCTTGGAG GAGTCGGACA TAATGGATGC ACTCAGGAGT 
TCCTGGGCTG AGAAAGAGAG TACATTAAAA AGATCAGAGA AGGGAGACAG GGAGCTCCTG 
AAGGCGGTGT TCCTGCTGGT TTACCACGAC TGTGCGCTCC CCCTGCTGCA CTCTGCGCTC 
CTGCCCCCGT CTAGATGGGC AGAGGAGGAG ACTGAAGCTG CCCGGTGGAA GGGCATCGCC 
GACTTCCTCA AGCAAAACCA GGAAAATGAG GGCGCCCTCC AGGTTCTGCT GTCGCCAGAG 
GGGGTCCACG AACCTTTCGA CATTTCCGAG CAGACCTATG ACTTCTTGGG TGAAAGAAGA 
AAGAACGTAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006059893.1
CDS	65..1939
Translation	MLARRQRDPLQSLQRRNQELKQQVDGLLFESRLKGALEASKRRDMYQRTGALTEREEEENSAEEDEEEEDDEEESEESGGEDEETEEEEEEKQEGESQPEATSGEYIALGDFMAQQVGDLTFKKGEILCIIKKNSDGWWMAKNAKGNEGLIPKTYLEPYNKEGGQESSEEGSEEDTEVGDGTVEGAEVKQRTDSHWSAVREAISEINTVDVLTAMGAIPAGFRPSTLFQLLEEGNQFRASYFLQPELTPSQLAFRDLMWDAKTGSIRSRPSRVSLILTLWSCKMIPLPGVSIQVLSRHVRLCLFDGSEVLSNIHTVRATWQPKKPKTWTFSPQVTGILPCLLDGDCFIRSNSSSPDLGILFELGISYIRNSTGERGELSCGWVFLKLFDTTGIPIPAKTYKLFLNGGTPYEKGVEVDPSVSTRAHGSVFHQLMTMRRQPQLLVKLRSLNRRSRSTLSLLPETLIGSMCCVHLLIFYRQILGDVLLKDRMSLNSADLISNPVLATFPKLLEESDIMDALRSSWAEKESTLKRSEKGDRELLKAVFLLVYHDCALPLLHSALLPPSRWAEEETEAARWKGIADFLKQNQENEGALQVLLSPEGVHEPFDISEQTYDFLGERRKNVA

Target ORF information:

RefSeq Version	XM_006059831.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephronophthisis 1 (juvenile) (NPHP1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006059831.1

ORF Insert Sequence:

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGAACTGGG GCACTTACTG AAAGAGAGGA AGAGGAAAAC 
TCTGCTGAAG AAGATGAGGA AGAAGAAGAC GATGAAGAAG AAAGCGAAGA AAGTGGGGGC 
GAGGACGAAG AAACAGAAGA GGAAGAGGAA GAGAAACAGG AAGGTGAATC TCAGCCAGAA 
GCCACGAGTG GAGAATACAT TGCTCTTGGA GATTTTATGG CTCAGCAAGT AGGAGACCTG 
ACATTTAAAA AAGGGGAAAT TCTCTGTATA ATCAAAAAAA ACTCTGATGG TTGGTGGATG 
GCTAAGAACG CCAAAGGAAA TGAAGGCCTT ATTCCTAAAA CCTACTTGGA GCCCTATAAT 
AAAGAAGGAG GTCAAGAATC CAGTGAAGAA GGCAGTGAAG AAGATACAGA GGTAGGGGAT 
GGAACAGTGG AAGGAGCCGA AGTCAAGCAA AGAACCGATT CTCACTGGAG TGCTGTCCGG 
GAAGCCATCT CAGAGATAAA CACCGTCGAC GTGCTGACCG CGATGGGAGC GATTCCTGCT 
GGGTTCAGGC CTTCCACGCT CTTCCAGCTC CTGGAGGAAG GGAATCAATT CCGAGCAAGC 
TACTTCTTAC AGCCAGAGCT CACACCTTCC CAACTGGCCT TCAGAGATCT GATGTGGGAT 
GCAAAAACCG GCTCTATCAG GTCAAGACCG AGCCGTGTCT CCTTGATTCT GACTCTCTGG 
AGCTGCAAGA TGATTCCTCT TCCAGGGGTG AGCATCCAAG TTCTGAGCAG ACACGTGCGC 
CTCTGTCTGT TTGACGGCAG TGAGGTTCTG AGCAACATTC ACACAGTCAG GGCCACGTGG 
CAACCTAAGA AGCCCAAAAC ATGGACCTTT TCTCCCCAGG TTACTGGCAT CTTGCCCTGC 
CTGCTTGATG GCGACTGTTT CATCAGATCC AACTCTTCCT CTCCAGATCT TGGAATATTA 
TTTGAACTTG GAATTTCTTA TATTCGCAAT TCCACTGGTG AAAGAGGAGA GTTAAGCTGT 
GGCTGGGTGT TTCTGAAACT TTTTGACACC ACTGGAATTC CTATTCCAGC AAAGACTTAC 
AAGCTCTTCT TGAACGGAGG CACCCCTTAT GAAAAAGGTG TTGAAGTGGA CCCTTCAGTA 
TCCACAAGAG CACATGGTAG TGTTTTCCAT CAGTTGATGA CAATGAGGAG GCAGCCTCAA 
CTTCTAGTCA AACTGAGATC TCTGAACAGA CGATCCAGGA GCACGCTAAG TCTACTACCA 
GAAACATTAA TCGGAAGCAT GTGCTGCGTT CATTTGTTGA TATTCTATCG CCAGATTCTT 
GGAGATGTTC TCCTGAAAGA CAGGATGAGC CTGAACAGTG CAGACTTAAT TAGTAATCCA 
GTGCTGGCTA CCTTCCCCAA GCTCTTGGAG GAGTCGGACA TAATGGATGC ACTCAGGAGT 
TCCTGGGCTG AGAAAGAGAG TACATTAAAA AGATCAGAGA AGGGAGACAG GGAGCTCCTG 
AAGGCGGTGT TCCTGCTGGT TTACCACGAC TGTGCGCTCC CCCTGCTGCA CTCTGCGCTC 
CTGCCCCCGT CTAGATGGGC AGAGGAGGAG ACTGAAGCTG CCCGGTGGAA GGGCATCGCC 
GACTTCCTCA AGCAAAACCA GGAAAATGAG GGCGCCCTCC AGGTTCTGCT GTCGCCAGAG 
GGGGTCCACG AACCTTTCGA CATTTCCGAG CAGACCTATG ACTTCTTGGG TGAAAGAAGA 
AAGAACGTAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP1 ( XM_025260600.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116385.1 Bubalus bubalis nephrocystin 1 (NPHP1), mRNA.

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CloneID	OBu45685
Clone ID Related Accession (Same CDS sequence)	XM_025260600.1
Accession Version	XM_025260600.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	LOW QUALITY PROTEIN: nephrocystin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGATGTATC CAGTTAAAAC AGGCAATAGA TGAAAATAAA 
AATGCTCTTC AAAAATTAAA AAAAGCTGAT GAACCTGCAC CTGTGGGCAA TTATTATCAG 
AAGAAAGAAG AGGAGCACAA TCTTTTGGAT AAGCTTACCC ACCAACTGCA GGAGCTTGCT 
GTGTCCATAA GCAGAGAAAA TGTCACTGAA ACTGGGGCAC TTACTGAAAG AGAGGAAGAG 
GAAAACTCTG CTGAAGAAGA TGAGGAAGAA GAAGACGATG AAGAAGAAAG CGAAGAAAGT 
GGGGGCGAGG ACGAAGAAAC AGAAGAGGAA GAGGAAGAGA AACAGGAAGG TGAATCTCAG 
CCAGAAGCCA CGAGTGGAGA ATACATTGCT CTTGGAGATT TTATGGCTCA GCAAGTAGGA 
GACCTGACAT TTAAAAAAGG GGAAATTCTC TGTATAATCA AAAAAAACTC TGATGGTTGG 
TGGATGGCTA AGAACGCCAA AGGAAATGAA GGCCTTATTC CTAAAACCTA CTTGGAGCCC 
TATAATAAAG AAGGAGGTCA AGAATCCAGT GAAGAAGGCA GTGAAGAAGA TACAGAGGTA 
GGGGATGGAA CAGTGGAAGG AGCCGAAGTC AAGCAAAGAA CCGATTCTCA CTGGAGTGCT 
GTCCGGAAGN CCATCTCAGA GGTTGGTATC TCCTGTCTTA TTAATAATGT GTCATTTTGT 
TACCTAATAG TTCTGATTAG AGTTCAGATT CAAAACAACT TTAAATGAAA GCATTGGATC 
TTAAGCGGGT ACAGGGCCTG GAATGCACAG GACATTAAAA CGTACCTCTG GTTTAGTAAG 
TCTGTACTCC GACAGATAAA CACCGTCGAC GTGCTGACCG CGATGGGAGC GATTCCTGCT 
GGGTTCAGGC CTTCCACGCT CTTCCAGCTC CTGGAGGAAG GGAATCAATT CCGAGCAAGC 
TACTTCTTAC AGCCAGAGCT CACACCTTCC CAACTGGCCT TCAGAGATCT GATGTGGGAT 
GCAAAAACCG GCTCTATCAG GTCAAGACCG AGCCGTGTCT CCTTGATTCT GACTCTCTGG 
AGCTGCAAGA TGATTCCTCT TCCAGGGGTG AGCATCCAAG TTCTGAGCAG ACACGTGCGC 
CTCTGTCTGT TTGACGGCAG TGAGGTTCTG AGCAACATTC ACACAGTCAG GGCCACGTGG 
CAACCTAAGA AGCCCAAAAC ATGGACCTTT TCTCCCCAGG TTACTGGCAT CTTGCCCTGC 
CTGCTTGATG GCGACTGTTT CATCAGATCC AACTCTTCCT CTCCAGATCT TGGAATATTA 
TTTGAACTTG GAATTTCTTA TATTCGCAAT TCCACTGGTG AAAGAGGAGA GTTAAGCTGT 
GGCTGGGTGT TTCTGAAACT TTTTGACACC ACTGGAATTC CTATTCCAGC AAAGACTTAC 
AAGCTCTTCT TGAACGGAGG CACCCCTTAT GAAAAAGGTG TTGAAGTGGA CCCTTCAGTA 
TCCACAAGAG CACATGGTAG TGTTTTCCAT CAGTTGATGA CAATGAGGAG GCAGCCTCAA 
CTTCTAGTCA AACTGAGATC TCTGAACAGA CGATCCAGGA GCACGCTAAG TCTACTACCA 
GAAACATTAA TCGGAAGCAT GTGCTGCGTT CATTTGTTGA TATTCTATCG CCAGATTCTT 
GGAGATGTTC TCCTGAAAGA CAGGATGAGC CTGAACAGTG CAGACTTAAT TAGTAATCCA 
GTGCTGGCTA CCTTCCCCAA GCTCTTGGAG GAGTCGGACA TAATGGATGC ACTCAGGAGT 
TCCTGGGCTG AGAAAGAGAG TACATTAAAA AGATCAGAGA AGGGAGACAG GGAGCTCCTG 
AAGGCGGTGT TCCTGCTGGT TTACCACGAC TGTGCGCTCC CCCTGCTGCA CTCTGCGCTC 
CTGCCCCCGT CTAGATGGGC AGAGGAGGAG ACTGAAGCTG CCCGGTGGAA GGGCATCGCC 
GACTTCCTCA AGCAAAACCA GGAAAATGAG GGCGCCCTCC AGGTTCTGCT GTCGCCAGAG 
GGGGTCCACG AACCTTTCGA CATTTCCGAG CAGACCTATG ACTTCTTGGG TGAAAGAAGA 
AAGAACGTAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025116385.1
CDS	140..2374
Translation	MLARRQRDPLQSLQRRNQELKQQVDGLLFESRLKGALEASKRRDMYQRCIQLKQAIDENKNALQKLKKADEPAPVGNYYQKKEEEHNLLDKLTHQLQELAVSISRENVTETGALTEREEEENSAEEDEEEEDDEEESEESGGEDEETEEEEEEKQEGESQPEATSGEYIALGDFMAQQVGDLTFKKGEILCIIKKNSDGWWMAKNAKGNEGLIPKTYLEPYNKEGGQESSEEGSEEDTEVGDGTVEGAEVKQRTDSHWSAVRKnullISEVGISCLINNVSFCYLIVLIRVQIQNNFK*KHWILSGYRAWNAQDIKTYLWFSKSVLRQINTVDVLTAMGAIPAGFRPSTLFQLLEEGNQFRASYFLQPELTPSQLAFRDLMWDAKTGSIRSRPSRVSLILTLWSCKMIPLPGVSIQVLSRHVRLCLFDGSEVLSNIHTVRATWQPKKPKTWTFSPQVTGILPCLLDGDCFIRSNSSSPDLGILFELGISYIRNSTGERGELSCGWVFLKLFDTTGIPIPAKTYKLFLNGGTPYEKGVEVDPSVSTRAHGSVFHQLMTMRRQPQLLVKLRSLNRRSRSTLSLLPETLIGSMCCVHLLIFYRQILGDVLLKDRMSLNSADLISNPVLATFPKLLEESDIMDALRSSWAEKESTLKRSEKGDRELLKAVFLLVYHDCALPLLHSALLPPSRWAEEETEAARWKGIADFLKQNQENEGALQVLLSPEGVHEPFDISEQTYDFLGERRKNVA

Target ORF information:

RefSeq Version	XM_025260600.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis nephrocystin 1 (NPHP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025260600.1

ORF Insert Sequence:

ATGCTGGCGA GGCGGCAGCG CGACCCACTC CAGTCCTTGC AGCGCCGCAA TCAGGAGCTG 
AAGCAACAGG TCGATGGGTT GCTTTTTGAG AGTCGACTGA AAGGAGCTCT AGAAGCAAGT 
AAAAGGAGAG ATATGTACCA AAGATGTATC CAGTTAAAAC AGGCAATAGA TGAAAATAAA 
AATGCTCTTC AAAAATTAAA AAAAGCTGAT GAACCTGCAC CTGTGGGCAA TTATTATCAG 
AAGAAAGAAG AGGAGCACAA TCTTTTGGAT AAGCTTACCC ACCAACTGCA GGAGCTTGCT 
GTGTCCATAA GCAGAGAAAA TGTCACTGAA ACTGGGGCAC TTACTGAAAG AGAGGAAGAG 
GAAAACTCTG CTGAAGAAGA TGAGGAAGAA GAAGACGATG AAGAAGAAAG CGAAGAAAGT 
GGGGGCGAGG ACGAAGAAAC AGAAGAGGAA GAGGAAGAGA AACAGGAAGG TGAATCTCAG 
CCAGAAGCCA CGAGTGGAGA ATACATTGCT CTTGGAGATT TTATGGCTCA GCAAGTAGGA 
GACCTGACAT TTAAAAAAGG GGAAATTCTC TGTATAATCA AAAAAAACTC TGATGGTTGG 
TGGATGGCTA AGAACGCCAA AGGAAATGAA GGCCTTATTC CTAAAACCTA CTTGGAGCCC 
TATAATAAAG AAGGAGGTCA AGAATCCAGT GAAGAAGGCA GTGAAGAAGA TACAGAGGTA 
GGGGATGGAA CAGTGGAAGG AGCCGAAGTC AAGCAAAGAA CCGATTCTCA CTGGAGTGCT 
GTCCGGAAGN CCATCTCAGA GGTTGGTATC TCCTGTCTTA TTAATAATGT GTCATTTTGT 
TACCTAATAG TTCTGATTAG AGTTCAGATT CAAAACAACT TTAAATGAAA GCATTGGATC 
TTAAGCGGGT ACAGGGCCTG GAATGCACAG GACATTAAAA CGTACCTCTG GTTTAGTAAG 
TCTGTACTCC GACAGATAAA CACCGTCGAC GTGCTGACCG CGATGGGAGC GATTCCTGCT 
GGGTTCAGGC CTTCCACGCT CTTCCAGCTC CTGGAGGAAG GGAATCAATT CCGAGCAAGC 
TACTTCTTAC AGCCAGAGCT CACACCTTCC CAACTGGCCT TCAGAGATCT GATGTGGGAT 
GCAAAAACCG GCTCTATCAG GTCAAGACCG AGCCGTGTCT CCTTGATTCT GACTCTCTGG 
AGCTGCAAGA TGATTCCTCT TCCAGGGGTG AGCATCCAAG TTCTGAGCAG ACACGTGCGC 
CTCTGTCTGT TTGACGGCAG TGAGGTTCTG AGCAACATTC ACACAGTCAG GGCCACGTGG 
CAACCTAAGA AGCCCAAAAC ATGGACCTTT TCTCCCCAGG TTACTGGCAT CTTGCCCTGC 
CTGCTTGATG GCGACTGTTT CATCAGATCC AACTCTTCCT CTCCAGATCT TGGAATATTA 
TTTGAACTTG GAATTTCTTA TATTCGCAAT TCCACTGGTG AAAGAGGAGA GTTAAGCTGT 
GGCTGGGTGT TTCTGAAACT TTTTGACACC ACTGGAATTC CTATTCCAGC AAAGACTTAC 
AAGCTCTTCT TGAACGGAGG CACCCCTTAT GAAAAAGGTG TTGAAGTGGA CCCTTCAGTA 
TCCACAAGAG CACATGGTAG TGTTTTCCAT CAGTTGATGA CAATGAGGAG GCAGCCTCAA 
CTTCTAGTCA AACTGAGATC TCTGAACAGA CGATCCAGGA GCACGCTAAG TCTACTACCA 
GAAACATTAA TCGGAAGCAT GTGCTGCGTT CATTTGTTGA TATTCTATCG CCAGATTCTT 
GGAGATGTTC TCCTGAAAGA CAGGATGAGC CTGAACAGTG CAGACTTAAT TAGTAATCCA 
GTGCTGGCTA CCTTCCCCAA GCTCTTGGAG GAGTCGGACA TAATGGATGC ACTCAGGAGT 
TCCTGGGCTG AGAAAGAGAG TACATTAAAA AGATCAGAGA AGGGAGACAG GGAGCTCCTG 
AAGGCGGTGT TCCTGCTGGT TTACCACGAC TGTGCGCTCC CCCTGCTGCA CTCTGCGCTC 
CTGCCCCCGT CTAGATGGGC AGAGGAGGAG ACTGAAGCTG CCCGGTGGAA GGGCATCGCC 
GACTTCCTCA AGCAAAACCA GGAAAATGAG GGCGCCCTCC AGGTTCTGCT GTCGCCAGAG 
GGGGTCCACG AACCTTTCGA CATTTCCGAG CAGACCTATG ACTTCTTGGG TGAAAGAAGA 
AAGAACGTAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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NPHP1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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