SP1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	SP1
Entrez Gene ID	102392142
Full Name	Sp1 transcription factor
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006047465.1	XP_006047527.1	transcription factor Sp1 isoform X2
XM_025284256.1	XP_025140041.1	transcription factor Sp1 isoform X3
XM_025284255.1	XP_025140040.1	transcription factor Sp1 isoform X2
XM_006047464.1	XP_006047526.1	transcription factor Sp1 isoform X1
XM_006047466.1	XP_006047528.1	transcription factor Sp1 isoform X3
XM_025284254.1	XP_025140039.1	transcription factor Sp1 isoform X1

Pathways from BioSystems
Gene Ontology

The following SP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu06067	XM_006047464.1 Latest version!	Bubalus bubalis Sp1 transcription factor (SP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu06067	XM_025284254.1 Latest version!	Bubalus bubalis Sp1 transcription factor (SP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu06068	XM_006047465.1 Latest version!	Bubalus bubalis Sp1 transcription factor (SP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu06068	XM_025284255.1 Latest version!	Bubalus bubalis Sp1 transcription factor (SP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu06069	XM_006047466.1 Latest version!	Bubalus bubalis Sp1 transcription factor (SP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu06069	XM_025284256.1 Latest version!	Bubalus bubalis Sp1 transcription factor (SP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SP1 ( XM_006047464.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047526.1 Bubalus bubalis Sp1 transcription factor (SP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu06067
Clone ID Related Accession (Same CDS sequence)	XM_006047464.1 , XM_025284254.1
Accession Version	XM_006047464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	transcription factor Sp1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784022.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGACC AAGATCACTC CATGGATGAA ATGACAGCAG TGGTGAAGAT TGAAAAAGGA 
GTTGGTGGCA GTAACGGGGG CAATGGAAAT GGTAGTGGTG CCTTTTCTCA GGCTCGAAGC 
AGCAGCGCAG GCAGCAGCAG CAGCAGCGGA GGAGGAGGGC AGGAATCCCA GCCATCCCCT 
TTGGCTCTGC TGGCAGCAAC TTGCAGCAGA ATTGAGTCAC CCAATGAAAA CAGCAACAAC 
TCCCAGGGCC CAAGCCAGTC AGGGGGCACA GGTGAGCTTG ACCTCACAGC CACACAACTT 
TCCCAGGGTG CCAATGGCTG GCAGATCATC TCTTCCTCCT CTGGGGCTAC CCCTACCTCG 
AAGGAACAGA GTGGCAGCAG TACCAACGGC AACAATGGCA GTGAGTCTTC CAAGAATCGC 
ACGGTCTCCG GTGGGCAGTA TGTTGTGGCT GCCACTTCCA ACCTTCAGAA CCAGCAAGTC 
CTGACCGGAC TACCTGGAGT GATGCCTAAT ATTCAGTATC AAGTAATCCC ACAGTTCCAG 
ACCGTTGATG GGCAACAGCT GCAGTTTGCT GCCACTGGGG CCCAAGTGCA GCAGGATGGT 
TCAGGTCAGA TACAGATCAT ACCAGGTGCA AACCAACAGA TCATCACAAA CCGAGGAAGT 
GGAGGCAACA TCATTGCTGC TATGCCAAAC CTACTCCAGC AGGCTGTCCC CCTTCAAGGC 
CTGGCTAATA ATGTACTCTC AGGACAGACT CAGTATGTGA CCAACGTACC AGTGGCCCTG 
AATGGGAATA TTACCTTGCT GCCTGTCAAC AGCGTTTCTG CAGCTACCTT GACTCCTAGC 
TCTCAGGCAG TCACGATCAG CAGCTCTGGG TCCCAGGAGA GTGGCTCACA GCCTGTCACC 
TCAGGGACTG CCATCAGTTC TGCCAGTTTG GTATCTTCAC AAGCCAGTTC CAGCTCCTTT 
TTCACCAATG CCAATAGCTA CTCAACAACT ACTACCACCA GCAACATGGG AATTATGAAC 
TTTACCACCA GCGGATCAGC AGGGACCAGC TCTCAGAGCC AGACACCCCA GAGGGTCAGC 
GGGCTGCAGG GATCTGATAC TCTGAACATC CAGCAGAACC AGACATCTGG AGGTTCACTG 
CAAGCAAGTC AACAAAAAGA GGGAGAGCAA AACCAGCAGA CACAGCAACA ACAACAAATT 
CTCATCCAGC CTCAGCTGGT TCAAGGGGGA CAGGCCCTTC AGGCCCTCCA AGCCACCCCA 
TTGTCAGGGC AGACCTTTAC AACTCAAGCT ATCACCCAGG AAACCCTCCA GAACCTTCAG 
CTTCAGGCTG TTCCAAACTC TGGCCCCATC ATCATCCGGA CACCAACAGT GGGGCCCAAT 
GGACAGGTCA GCTGGCAGAC TCTTCAGCTG CAGAACCTCC AAGTTCAGAA CCCACAAGCC 
CAGACAATCA CCTTGGCCCC AATGCAGGGT GTTTCTTTGG GGCAGACCAG CAGCAGCAGT 
ACCACCCTTA CGCCTATTGC CTCAGCTGCC TCCATCCCTG CCGGCACAGT CACTGTGAAT 
GCTGCTCAGC TCTCCTCCAT GCCTGGCCTC CAGACCATTA ACCTCAGTGC ATTGGGTGCT 
CCAGGAATCC AGGTGCACCA GCTTCCAAGC CTACCCTTGG CAATAGCAAA TGCCTCAGGT 
GATCATGGAG CTCAGCTTGG CCTCCATGGG GCTGGTGGTG ATGGAATACA TGATGACCCA 
ACAGGGGGAG AAGAAGGAGA GAACAGCCCA GATCCCCAAC CCCAAGCTGG TCGGAGGACC 
CGACGGGAAG CATGCACCTG CCCCTACTGT AAAGACAGTG AAGGAAGGGG CTCTGGGGAT 
CCTGGCAAGA AGAAACAGCA CATTTGTCAC ATGCAAGGCT GTGGCAAAGT ATATGGCAAG 
ACCTCACACC TACGGGCACA CTTGCGCTGG CATACGGGCG AGAGGCCTTT CATGTGTAAC 
TGGTCATTCT GTGGGAAACG CTTCACACGT TCAGATGAGT TACAGAGGCA CAAACGCACA 
CACACAGGTG AGAAGAAATT TGCCTGCCCT GAGTGTCCGA AGCGCTTCAT GAGGAGTGAC 
CACCTGTCAA AGCATATCAA GACCCACCAG AATAAGAAGG GAGGCCCGGG TGTAGCCCTG 
AGTGTGGGCA CTTTGCCCCT GGACAGTGGG GCAGGTTCAG AAGGCAGTGG TACTGCCACC 
CCTTCAGCCC TTATTACCAC CAACATGGTA GCCATGGAGG CCATTTGTCC GGAGGGTATT 
GCCCGTCTTG CCAACAGTGG CATCAATGTC ATGCAGGTGG CAGATCTGCA GTCCATTAAT 
ATCAGTGGCA ATGGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047526.1
CDS	83..2443
Translation	MSDQDHSMDEMTAVVKIEKGVGGSNGGNGNGSGAFSQARSSSAGSSSSSGGGGQESQPSPLALLAATCSRIESPNENSNNSQGPSQSGGTGELDLTATQLSQGANGWQIISSSSGATPTSKEQSGSSTNGNNGSESSKNRTVSGGQYVVAATSNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQDGSGQIQIIPGANQQIITNRGSGGNIIAAMPNLLQQAVPLQGLANNVLSGQTQYVTNVPVALNGNITLLPVNSVSAATLTPSSQAVTISSSGSQESGSQPVTSGTAISSASLVSSQASSSSFFTNANSYSTTTTTSNMGIMNFTTSGSAGTSSQSQTPQRVSGLQGSDTLNIQQNQTSGGSLQASQQKEGEQNQQTQQQQQILIQPQLVQGGQALQALQATPLSGQTFTTQAITQETLQNLQLQAVPNSGPIIIRTPTVGPNGQVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQTSSSSTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGAPGIQVHQLPSLPLAIANASGDHGAQLGLHGAGGDGIHDDPTGGEEGENSPDPQPQAGRRTRREACTCPYCKDSEGRGSGDPGKKKQHICHMQGCGKVYGKTSHLRAHLRWHTGERPFMCNWSFCGKRFTRSDELQRHKRTHTGEKKFACPECPKRFMRSDHLSKHIKTHQNKKGGPGVALSVGTLPLDSGAGSEGSGTATPSALITTNMVAMEAICPEGIARLANSGINVMQVADLQSINISGNGF

Target ORF information:

RefSeq Version	XM_006047464.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis Sp1 transcription factor (SP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047464.1

ORF Insert Sequence:

ATGAGCGACC AAGATCACTC CATGGATGAA ATGACAGCAG TGGTGAAGAT TGAAAAAGGA 
GTTGGTGGCA GTAACGGGGG CAATGGAAAT GGTAGTGGTG CCTTTTCTCA GGCTCGAAGC 
AGCAGCGCAG GCAGCAGCAG CAGCAGCGGA GGAGGAGGGC AGGAATCCCA GCCATCCCCT 
TTGGCTCTGC TGGCAGCAAC TTGCAGCAGA ATTGAGTCAC CCAATGAAAA CAGCAACAAC 
TCCCAGGGCC CAAGCCAGTC AGGGGGCACA GGTGAGCTTG ACCTCACAGC CACACAACTT 
TCCCAGGGTG CCAATGGCTG GCAGATCATC TCTTCCTCCT CTGGGGCTAC CCCTACCTCG 
AAGGAACAGA GTGGCAGCAG TACCAACGGC AACAATGGCA GTGAGTCTTC CAAGAATCGC 
ACGGTCTCCG GTGGGCAGTA TGTTGTGGCT GCCACTTCCA ACCTTCAGAA CCAGCAAGTC 
CTGACCGGAC TACCTGGAGT GATGCCTAAT ATTCAGTATC AAGTAATCCC ACAGTTCCAG 
ACCGTTGATG GGCAACAGCT GCAGTTTGCT GCCACTGGGG CCCAAGTGCA GCAGGATGGT 
TCAGGTCAGA TACAGATCAT ACCAGGTGCA AACCAACAGA TCATCACAAA CCGAGGAAGT 
GGAGGCAACA TCATTGCTGC TATGCCAAAC CTACTCCAGC AGGCTGTCCC CCTTCAAGGC 
CTGGCTAATA ATGTACTCTC AGGACAGACT CAGTATGTGA CCAACGTACC AGTGGCCCTG 
AATGGGAATA TTACCTTGCT GCCTGTCAAC AGCGTTTCTG CAGCTACCTT GACTCCTAGC 
TCTCAGGCAG TCACGATCAG CAGCTCTGGG TCCCAGGAGA GTGGCTCACA GCCTGTCACC 
TCAGGGACTG CCATCAGTTC TGCCAGTTTG GTATCTTCAC AAGCCAGTTC CAGCTCCTTT 
TTCACCAATG CCAATAGCTA CTCAACAACT ACTACCACCA GCAACATGGG AATTATGAAC 
TTTACCACCA GCGGATCAGC AGGGACCAGC TCTCAGAGCC AGACACCCCA GAGGGTCAGC 
GGGCTGCAGG GATCTGATAC TCTGAACATC CAGCAGAACC AGACATCTGG AGGTTCACTG 
CAAGCAAGTC AACAAAAAGA GGGAGAGCAA AACCAGCAGA CACAGCAACA ACAACAAATT 
CTCATCCAGC CTCAGCTGGT TCAAGGGGGA CAGGCCCTTC AGGCCCTCCA AGCCACCCCA 
TTGTCAGGGC AGACCTTTAC AACTCAAGCT ATCACCCAGG AAACCCTCCA GAACCTTCAG 
CTTCAGGCTG TTCCAAACTC TGGCCCCATC ATCATCCGGA CACCAACAGT GGGGCCCAAT 
GGACAGGTCA GCTGGCAGAC TCTTCAGCTG CAGAACCTCC AAGTTCAGAA CCCACAAGCC 
CAGACAATCA CCTTGGCCCC AATGCAGGGT GTTTCTTTGG GGCAGACCAG CAGCAGCAGT 
ACCACCCTTA CGCCTATTGC CTCAGCTGCC TCCATCCCTG CCGGCACAGT CACTGTGAAT 
GCTGCTCAGC TCTCCTCCAT GCCTGGCCTC CAGACCATTA ACCTCAGTGC ATTGGGTGCT 
CCAGGAATCC AGGTGCACCA GCTTCCAAGC CTACCCTTGG CAATAGCAAA TGCCTCAGGT 
GATCATGGAG CTCAGCTTGG CCTCCATGGG GCTGGTGGTG ATGGAATACA TGATGACCCA 
ACAGGGGGAG AAGAAGGAGA GAACAGCCCA GATCCCCAAC CCCAAGCTGG TCGGAGGACC 
CGACGGGAAG CATGCACCTG CCCCTACTGT AAAGACAGTG AAGGAAGGGG CTCTGGGGAT 
CCTGGCAAGA AGAAACAGCA CATTTGTCAC ATGCAAGGCT GTGGCAAAGT ATATGGCAAG 
ACCTCACACC TACGGGCACA CTTGCGCTGG CATACGGGCG AGAGGCCTTT CATGTGTAAC 
TGGTCATTCT GTGGGAAACG CTTCACACGT TCAGATGAGT TACAGAGGCA CAAACGCACA 
CACACAGGTG AGAAGAAATT TGCCTGCCCT GAGTGTCCGA AGCGCTTCAT GAGGAGTGAC 
CACCTGTCAA AGCATATCAA GACCCACCAG AATAAGAAGG GAGGCCCGGG TGTAGCCCTG 
AGTGTGGGCA CTTTGCCCCT GGACAGTGGG GCAGGTTCAG AAGGCAGTGG TACTGCCACC 
CCTTCAGCCC TTATTACCAC CAACATGGTA GCCATGGAGG CCATTTGTCC GGAGGGTATT 
GCCCGTCTTG CCAACAGTGG CATCAATGTC ATGCAGGTGG CAGATCTGCA GTCCATTAAT 
ATCAGTGGCA ATGGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SP1 ( XM_025284254.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025140039.1 Bubalus bubalis Sp1 transcription factor (SP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu06067
Clone ID Related Accession (Same CDS sequence)	XM_006047464.1 , XM_025284254.1
Accession Version	XM_025284254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	transcription factor Sp1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGACC AAGATCACTC CATGGATGAA ATGACAGCAG TGGTGAAGAT TGAAAAAGGA 
GTTGGTGGCA GTAACGGGGG CAATGGAAAT GGTAGTGGTG CCTTTTCTCA GGCTCGAAGC 
AGCAGCGCAG GCAGCAGCAG CAGCAGCGGA GGAGGAGGGC AGGAATCCCA GCCATCCCCT 
TTGGCTCTGC TGGCAGCAAC TTGCAGCAGA ATTGAGTCAC CCAATGAAAA CAGCAACAAC 
TCCCAGGGCC CAAGCCAGTC AGGGGGCACA GGTGAGCTTG ACCTCACAGC CACACAACTT 
TCCCAGGGTG CCAATGGCTG GCAGATCATC TCTTCCTCCT CTGGGGCTAC CCCTACCTCG 
AAGGAACAGA GTGGCAGCAG TACCAACGGC AACAATGGCA GTGAGTCTTC CAAGAATCGC 
ACGGTCTCCG GTGGGCAGTA TGTTGTGGCT GCCACTTCCA ACCTTCAGAA CCAGCAAGTC 
CTGACCGGAC TACCTGGAGT GATGCCTAAT ATTCAGTATC AAGTAATCCC ACAGTTCCAG 
ACCGTTGATG GGCAACAGCT GCAGTTTGCT GCCACTGGGG CCCAAGTGCA GCAGGATGGT 
TCAGGTCAGA TACAGATCAT ACCAGGTGCA AACCAACAGA TCATCACAAA CCGAGGAAGT 
GGAGGCAACA TCATTGCTGC TATGCCAAAC CTACTCCAGC AGGCTGTCCC CCTTCAAGGC 
CTGGCTAATA ATGTACTCTC AGGACAGACT CAGTATGTGA CCAACGTACC AGTGGCCCTG 
AATGGGAATA TTACCTTGCT GCCTGTCAAC AGCGTTTCTG CAGCTACCTT GACTCCTAGC 
TCTCAGGCAG TCACGATCAG CAGCTCTGGG TCCCAGGAGA GTGGCTCACA GCCTGTCACC 
TCAGGGACTG CCATCAGTTC TGCCAGTTTG GTATCTTCAC AAGCCAGTTC CAGCTCCTTT 
TTCACCAATG CCAATAGCTA CTCAACAACT ACTACCACCA GCAACATGGG AATTATGAAC 
TTTACCACCA GCGGATCAGC AGGGACCAGC TCTCAGAGCC AGACACCCCA GAGGGTCAGC 
GGGCTGCAGG GATCTGATAC TCTGAACATC CAGCAGAACC AGACATCTGG AGGTTCACTG 
CAAGCAAGTC AACAAAAAGA GGGAGAGCAA AACCAGCAGA CACAGCAACA ACAACAAATT 
CTCATCCAGC CTCAGCTGGT TCAAGGGGGA CAGGCCCTTC AGGCCCTCCA AGCCACCCCA 
TTGTCAGGGC AGACCTTTAC AACTCAAGCT ATCACCCAGG AAACCCTCCA GAACCTTCAG 
CTTCAGGCTG TTCCAAACTC TGGCCCCATC ATCATCCGGA CACCAACAGT GGGGCCCAAT 
GGACAGGTCA GCTGGCAGAC TCTTCAGCTG CAGAACCTCC AAGTTCAGAA CCCACAAGCC 
CAGACAATCA CCTTGGCCCC AATGCAGGGT GTTTCTTTGG GGCAGACCAG CAGCAGCAGT 
ACCACCCTTA CGCCTATTGC CTCAGCTGCC TCCATCCCTG CCGGCACAGT CACTGTGAAT 
GCTGCTCAGC TCTCCTCCAT GCCTGGCCTC CAGACCATTA ACCTCAGTGC ATTGGGTGCT 
CCAGGAATCC AGGTGCACCA GCTTCCAAGC CTACCCTTGG CAATAGCAAA TGCCTCAGGT 
GATCATGGAG CTCAGCTTGG CCTCCATGGG GCTGGTGGTG ATGGAATACA TGATGACCCA 
ACAGGGGGAG AAGAAGGAGA GAACAGCCCA GATCCCCAAC CCCAAGCTGG TCGGAGGACC 
CGACGGGAAG CATGCACCTG CCCCTACTGT AAAGACAGTG AAGGAAGGGG CTCTGGGGAT 
CCTGGCAAGA AGAAACAGCA CATTTGTCAC ATGCAAGGCT GTGGCAAAGT ATATGGCAAG 
ACCTCACACC TACGGGCACA CTTGCGCTGG CATACGGGCG AGAGGCCTTT CATGTGTAAC 
TGGTCATTCT GTGGGAAACG CTTCACACGT TCAGATGAGT TACAGAGGCA CAAACGCACA 
CACACAGGTG AGAAGAAATT TGCCTGCCCT GAGTGTCCGA AGCGCTTCAT GAGGAGTGAC 
CACCTGTCAA AGCATATCAA GACCCACCAG AATAAGAAGG GAGGCCCGGG TGTAGCCCTG 
AGTGTGGGCA CTTTGCCCCT GGACAGTGGG GCAGGTTCAG AAGGCAGTGG TACTGCCACC 
CCTTCAGCCC TTATTACCAC CAACATGGTA GCCATGGAGG CCATTTGTCC GGAGGGTATT 
GCCCGTCTTG CCAACAGTGG CATCAATGTC ATGCAGGTGG CAGATCTGCA GTCCATTAAT 
ATCAGTGGCA ATGGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025140039.1
CDS	113..2473
Translation	MSDQDHSMDEMTAVVKIEKGVGGSNGGNGNGSGAFSQARSSSAGSSSSSGGGGQESQPSPLALLAATCSRIESPNENSNNSQGPSQSGGTGELDLTATQLSQGANGWQIISSSSGATPTSKEQSGSSTNGNNGSESSKNRTVSGGQYVVAATSNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQDGSGQIQIIPGANQQIITNRGSGGNIIAAMPNLLQQAVPLQGLANNVLSGQTQYVTNVPVALNGNITLLPVNSVSAATLTPSSQAVTISSSGSQESGSQPVTSGTAISSASLVSSQASSSSFFTNANSYSTTTTTSNMGIMNFTTSGSAGTSSQSQTPQRVSGLQGSDTLNIQQNQTSGGSLQASQQKEGEQNQQTQQQQQILIQPQLVQGGQALQALQATPLSGQTFTTQAITQETLQNLQLQAVPNSGPIIIRTPTVGPNGQVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQTSSSSTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGAPGIQVHQLPSLPLAIANASGDHGAQLGLHGAGGDGIHDDPTGGEEGENSPDPQPQAGRRTRREACTCPYCKDSEGRGSGDPGKKKQHICHMQGCGKVYGKTSHLRAHLRWHTGERPFMCNWSFCGKRFTRSDELQRHKRTHTGEKKFACPECPKRFMRSDHLSKHIKTHQNKKGGPGVALSVGTLPLDSGAGSEGSGTATPSALITTNMVAMEAICPEGIARLANSGINVMQVADLQSINISGNGF

Target ORF information:

RefSeq Version	XM_025284254.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis Sp1 transcription factor (SP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025284254.1

ORF Insert Sequence:

ATGAGCGACC AAGATCACTC CATGGATGAA ATGACAGCAG TGGTGAAGAT TGAAAAAGGA 
GTTGGTGGCA GTAACGGGGG CAATGGAAAT GGTAGTGGTG CCTTTTCTCA GGCTCGAAGC 
AGCAGCGCAG GCAGCAGCAG CAGCAGCGGA GGAGGAGGGC AGGAATCCCA GCCATCCCCT 
TTGGCTCTGC TGGCAGCAAC TTGCAGCAGA ATTGAGTCAC CCAATGAAAA CAGCAACAAC 
TCCCAGGGCC CAAGCCAGTC AGGGGGCACA GGTGAGCTTG ACCTCACAGC CACACAACTT 
TCCCAGGGTG CCAATGGCTG GCAGATCATC TCTTCCTCCT CTGGGGCTAC CCCTACCTCG 
AAGGAACAGA GTGGCAGCAG TACCAACGGC AACAATGGCA GTGAGTCTTC CAAGAATCGC 
ACGGTCTCCG GTGGGCAGTA TGTTGTGGCT GCCACTTCCA ACCTTCAGAA CCAGCAAGTC 
CTGACCGGAC TACCTGGAGT GATGCCTAAT ATTCAGTATC AAGTAATCCC ACAGTTCCAG 
ACCGTTGATG GGCAACAGCT GCAGTTTGCT GCCACTGGGG CCCAAGTGCA GCAGGATGGT 
TCAGGTCAGA TACAGATCAT ACCAGGTGCA AACCAACAGA TCATCACAAA CCGAGGAAGT 
GGAGGCAACA TCATTGCTGC TATGCCAAAC CTACTCCAGC AGGCTGTCCC CCTTCAAGGC 
CTGGCTAATA ATGTACTCTC AGGACAGACT CAGTATGTGA CCAACGTACC AGTGGCCCTG 
AATGGGAATA TTACCTTGCT GCCTGTCAAC AGCGTTTCTG CAGCTACCTT GACTCCTAGC 
TCTCAGGCAG TCACGATCAG CAGCTCTGGG TCCCAGGAGA GTGGCTCACA GCCTGTCACC 
TCAGGGACTG CCATCAGTTC TGCCAGTTTG GTATCTTCAC AAGCCAGTTC CAGCTCCTTT 
TTCACCAATG CCAATAGCTA CTCAACAACT ACTACCACCA GCAACATGGG AATTATGAAC 
TTTACCACCA GCGGATCAGC AGGGACCAGC TCTCAGAGCC AGACACCCCA GAGGGTCAGC 
GGGCTGCAGG GATCTGATAC TCTGAACATC CAGCAGAACC AGACATCTGG AGGTTCACTG 
CAAGCAAGTC AACAAAAAGA GGGAGAGCAA AACCAGCAGA CACAGCAACA ACAACAAATT 
CTCATCCAGC CTCAGCTGGT TCAAGGGGGA CAGGCCCTTC AGGCCCTCCA AGCCACCCCA 
TTGTCAGGGC AGACCTTTAC AACTCAAGCT ATCACCCAGG AAACCCTCCA GAACCTTCAG 
CTTCAGGCTG TTCCAAACTC TGGCCCCATC ATCATCCGGA CACCAACAGT GGGGCCCAAT 
GGACAGGTCA GCTGGCAGAC TCTTCAGCTG CAGAACCTCC AAGTTCAGAA CCCACAAGCC 
CAGACAATCA CCTTGGCCCC AATGCAGGGT GTTTCTTTGG GGCAGACCAG CAGCAGCAGT 
ACCACCCTTA CGCCTATTGC CTCAGCTGCC TCCATCCCTG CCGGCACAGT CACTGTGAAT 
GCTGCTCAGC TCTCCTCCAT GCCTGGCCTC CAGACCATTA ACCTCAGTGC ATTGGGTGCT 
CCAGGAATCC AGGTGCACCA GCTTCCAAGC CTACCCTTGG CAATAGCAAA TGCCTCAGGT 
GATCATGGAG CTCAGCTTGG CCTCCATGGG GCTGGTGGTG ATGGAATACA TGATGACCCA 
ACAGGGGGAG AAGAAGGAGA GAACAGCCCA GATCCCCAAC CCCAAGCTGG TCGGAGGACC 
CGACGGGAAG CATGCACCTG CCCCTACTGT AAAGACAGTG AAGGAAGGGG CTCTGGGGAT 
CCTGGCAAGA AGAAACAGCA CATTTGTCAC ATGCAAGGCT GTGGCAAAGT ATATGGCAAG 
ACCTCACACC TACGGGCACA CTTGCGCTGG CATACGGGCG AGAGGCCTTT CATGTGTAAC 
TGGTCATTCT GTGGGAAACG CTTCACACGT TCAGATGAGT TACAGAGGCA CAAACGCACA 
CACACAGGTG AGAAGAAATT TGCCTGCCCT GAGTGTCCGA AGCGCTTCAT GAGGAGTGAC 
CACCTGTCAA AGCATATCAA GACCCACCAG AATAAGAAGG GAGGCCCGGG TGTAGCCCTG 
AGTGTGGGCA CTTTGCCCCT GGACAGTGGG GCAGGTTCAG AAGGCAGTGG TACTGCCACC 
CCTTCAGCCC TTATTACCAC CAACATGGTA GCCATGGAGG CCATTTGTCC GGAGGGTATT 
GCCCGTCTTG CCAACAGTGG CATCAATGTC ATGCAGGTGG CAGATCTGCA GTCCATTAAT 
ATCAGTGGCA ATGGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SP1 ( XM_006047465.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047527.1 Bubalus bubalis Sp1 transcription factor (SP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu06068
Clone ID Related Accession (Same CDS sequence)	XM_006047465.1 , XM_025284255.1
Accession Version	XM_006047465.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	transcription factor Sp1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784022.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAA TGACAGCAGT GGTGAAGATT GAAAAAGGAG TTGGTGGCAG TAACGGGGGC 
AATGGAAATG GTAGTGGTGC CTTTTCTCAG GCTCGAAGCA GCAGCGCAGG CAGCAGCAGC 
AGCAGCGGAG GAGGAGGGCA GGAATCCCAG CCATCCCCTT TGGCTCTGCT GGCAGCAACT 
TGCAGCAGAA TTGAGTCACC CAATGAAAAC AGCAACAACT CCCAGGGCCC AAGCCAGTCA 
GGGGGCACAG GTGAGCTTGA CCTCACAGCC ACACAACTTT CCCAGGGTGC CAATGGCTGG 
CAGATCATCT CTTCCTCCTC TGGGGCTACC CCTACCTCGA AGGAACAGAG TGGCAGCAGT 
ACCAACGGCA ACAATGGCAG TGAGTCTTCC AAGAATCGCA CGGTCTCCGG TGGGCAGTAT 
GTTGTGGCTG CCACTTCCAA CCTTCAGAAC CAGCAAGTCC TGACCGGACT ACCTGGAGTG 
ATGCCTAATA TTCAGTATCA AGTAATCCCA CAGTTCCAGA CCGTTGATGG GCAACAGCTG 
CAGTTTGCTG CCACTGGGGC CCAAGTGCAG CAGGATGGTT CAGGTCAGAT ACAGATCATA 
CCAGGTGCAA ACCAACAGAT CATCACAAAC CGAGGAAGTG GAGGCAACAT CATTGCTGCT 
ATGCCAAACC TACTCCAGCA GGCTGTCCCC CTTCAAGGCC TGGCTAATAA TGTACTCTCA 
GGACAGACTC AGTATGTGAC CAACGTACCA GTGGCCCTGA ATGGGAATAT TACCTTGCTG 
CCTGTCAACA GCGTTTCTGC AGCTACCTTG ACTCCTAGCT CTCAGGCAGT CACGATCAGC 
AGCTCTGGGT CCCAGGAGAG TGGCTCACAG CCTGTCACCT CAGGGACTGC CATCAGTTCT 
GCCAGTTTGG TATCTTCACA AGCCAGTTCC AGCTCCTTTT TCACCAATGC CAATAGCTAC 
TCAACAACTA CTACCACCAG CAACATGGGA ATTATGAACT TTACCACCAG CGGATCAGCA 
GGGACCAGCT CTCAGAGCCA GACACCCCAG AGGGTCAGCG GGCTGCAGGG ATCTGATACT 
CTGAACATCC AGCAGAACCA GACATCTGGA GGTTCACTGC AAGCAAGTCA ACAAAAAGAG 
GGAGAGCAAA ACCAGCAGAC ACAGCAACAA CAACAAATTC TCATCCAGCC TCAGCTGGTT 
CAAGGGGGAC AGGCCCTTCA GGCCCTCCAA GCCACCCCAT TGTCAGGGCA GACCTTTACA 
ACTCAAGCTA TCACCCAGGA AACCCTCCAG AACCTTCAGC TTCAGGCTGT TCCAAACTCT 
GGCCCCATCA TCATCCGGAC ACCAACAGTG GGGCCCAATG GACAGGTCAG CTGGCAGACT 
CTTCAGCTGC AGAACCTCCA AGTTCAGAAC CCACAAGCCC AGACAATCAC CTTGGCCCCA 
ATGCAGGGTG TTTCTTTGGG GCAGACCAGC AGCAGCAGTA CCACCCTTAC GCCTATTGCC 
TCAGCTGCCT CCATCCCTGC CGGCACAGTC ACTGTGAATG CTGCTCAGCT CTCCTCCATG 
CCTGGCCTCC AGACCATTAA CCTCAGTGCA TTGGGTGCTC CAGGAATCCA GGTGCACCAG 
CTTCCAAGCC TACCCTTGGC AATAGCAAAT GCCTCAGGTG ATCATGGAGC TCAGCTTGGC 
CTCCATGGGG CTGGTGGTGA TGGAATACAT GATGACCCAA CAGGGGGAGA AGAAGGAGAG 
AACAGCCCAG ATCCCCAACC CCAAGCTGGT CGGAGGACCC GACGGGAAGC ATGCACCTGC 
CCCTACTGTA AAGACAGTGA AGGAAGGGGC TCTGGGGATC CTGGCAAGAA GAAACAGCAC 
ATTTGTCACA TGCAAGGCTG TGGCAAAGTA TATGGCAAGA CCTCACACCT ACGGGCACAC 
TTGCGCTGGC ATACGGGCGA GAGGCCTTTC ATGTGTAACT GGTCATTCTG TGGGAAACGC 
TTCACACGTT CAGATGAGTT ACAGAGGCAC AAACGCACAC ACACAGGTGA GAAGAAATTT 
GCCTGCCCTG AGTGTCCGAA GCGCTTCATG AGGAGTGACC ACCTGTCAAA GCATATCAAG 
ACCCACCAGA ATAAGAAGGG AGGCCCGGGT GTAGCCCTGA GTGTGGGCAC TTTGCCCCTG 
GACAGTGGGG CAGGTTCAGA AGGCAGTGGT ACTGCCACCC CTTCAGCCCT TATTACCACC 
AACATGGTAG CCATGGAGGC CATTTGTCCG GAGGGTATTG CCCGTCTTGC CAACAGTGGC 
ATCAATGTCA TGCAGGTGGC AGATCTGCAG TCCATTAATA TCAGTGGCAA TGGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047527.1
CDS	459..2798
Translation	MDEMTAVVKIEKGVGGSNGGNGNGSGAFSQARSSSAGSSSSSGGGGQESQPSPLALLAATCSRIESPNENSNNSQGPSQSGGTGELDLTATQLSQGANGWQIISSSSGATPTSKEQSGSSTNGNNGSESSKNRTVSGGQYVVAATSNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQDGSGQIQIIPGANQQIITNRGSGGNIIAAMPNLLQQAVPLQGLANNVLSGQTQYVTNVPVALNGNITLLPVNSVSAATLTPSSQAVTISSSGSQESGSQPVTSGTAISSASLVSSQASSSSFFTNANSYSTTTTTSNMGIMNFTTSGSAGTSSQSQTPQRVSGLQGSDTLNIQQNQTSGGSLQASQQKEGEQNQQTQQQQQILIQPQLVQGGQALQALQATPLSGQTFTTQAITQETLQNLQLQAVPNSGPIIIRTPTVGPNGQVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQTSSSSTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGAPGIQVHQLPSLPLAIANASGDHGAQLGLHGAGGDGIHDDPTGGEEGENSPDPQPQAGRRTRREACTCPYCKDSEGRGSGDPGKKKQHICHMQGCGKVYGKTSHLRAHLRWHTGERPFMCNWSFCGKRFTRSDELQRHKRTHTGEKKFACPECPKRFMRSDHLSKHIKTHQNKKGGPGVALSVGTLPLDSGAGSEGSGTATPSALITTNMVAMEAICPEGIARLANSGINVMQVADLQSINISGNGF

Target ORF information:

RefSeq Version	XM_006047465.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis Sp1 transcription factor (SP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047465.1

ORF Insert Sequence:

ATGGATGAAA TGACAGCAGT GGTGAAGATT GAAAAAGGAG TTGGTGGCAG TAACGGGGGC 
AATGGAAATG GTAGTGGTGC CTTTTCTCAG GCTCGAAGCA GCAGCGCAGG CAGCAGCAGC 
AGCAGCGGAG GAGGAGGGCA GGAATCCCAG CCATCCCCTT TGGCTCTGCT GGCAGCAACT 
TGCAGCAGAA TTGAGTCACC CAATGAAAAC AGCAACAACT CCCAGGGCCC AAGCCAGTCA 
GGGGGCACAG GTGAGCTTGA CCTCACAGCC ACACAACTTT CCCAGGGTGC CAATGGCTGG 
CAGATCATCT CTTCCTCCTC TGGGGCTACC CCTACCTCGA AGGAACAGAG TGGCAGCAGT 
ACCAACGGCA ACAATGGCAG TGAGTCTTCC AAGAATCGCA CGGTCTCCGG TGGGCAGTAT 
GTTGTGGCTG CCACTTCCAA CCTTCAGAAC CAGCAAGTCC TGACCGGACT ACCTGGAGTG 
ATGCCTAATA TTCAGTATCA AGTAATCCCA CAGTTCCAGA CCGTTGATGG GCAACAGCTG 
CAGTTTGCTG CCACTGGGGC CCAAGTGCAG CAGGATGGTT CAGGTCAGAT ACAGATCATA 
CCAGGTGCAA ACCAACAGAT CATCACAAAC CGAGGAAGTG GAGGCAACAT CATTGCTGCT 
ATGCCAAACC TACTCCAGCA GGCTGTCCCC CTTCAAGGCC TGGCTAATAA TGTACTCTCA 
GGACAGACTC AGTATGTGAC CAACGTACCA GTGGCCCTGA ATGGGAATAT TACCTTGCTG 
CCTGTCAACA GCGTTTCTGC AGCTACCTTG ACTCCTAGCT CTCAGGCAGT CACGATCAGC 
AGCTCTGGGT CCCAGGAGAG TGGCTCACAG CCTGTCACCT CAGGGACTGC CATCAGTTCT 
GCCAGTTTGG TATCTTCACA AGCCAGTTCC AGCTCCTTTT TCACCAATGC CAATAGCTAC 
TCAACAACTA CTACCACCAG CAACATGGGA ATTATGAACT TTACCACCAG CGGATCAGCA 
GGGACCAGCT CTCAGAGCCA GACACCCCAG AGGGTCAGCG GGCTGCAGGG ATCTGATACT 
CTGAACATCC AGCAGAACCA GACATCTGGA GGTTCACTGC AAGCAAGTCA ACAAAAAGAG 
GGAGAGCAAA ACCAGCAGAC ACAGCAACAA CAACAAATTC TCATCCAGCC TCAGCTGGTT 
CAAGGGGGAC AGGCCCTTCA GGCCCTCCAA GCCACCCCAT TGTCAGGGCA GACCTTTACA 
ACTCAAGCTA TCACCCAGGA AACCCTCCAG AACCTTCAGC TTCAGGCTGT TCCAAACTCT 
GGCCCCATCA TCATCCGGAC ACCAACAGTG GGGCCCAATG GACAGGTCAG CTGGCAGACT 
CTTCAGCTGC AGAACCTCCA AGTTCAGAAC CCACAAGCCC AGACAATCAC CTTGGCCCCA 
ATGCAGGGTG TTTCTTTGGG GCAGACCAGC AGCAGCAGTA CCACCCTTAC GCCTATTGCC 
TCAGCTGCCT CCATCCCTGC CGGCACAGTC ACTGTGAATG CTGCTCAGCT CTCCTCCATG 
CCTGGCCTCC AGACCATTAA CCTCAGTGCA TTGGGTGCTC CAGGAATCCA GGTGCACCAG 
CTTCCAAGCC TACCCTTGGC AATAGCAAAT GCCTCAGGTG ATCATGGAGC TCAGCTTGGC 
CTCCATGGGG CTGGTGGTGA TGGAATACAT GATGACCCAA CAGGGGGAGA AGAAGGAGAG 
AACAGCCCAG ATCCCCAACC CCAAGCTGGT CGGAGGACCC GACGGGAAGC ATGCACCTGC 
CCCTACTGTA AAGACAGTGA AGGAAGGGGC TCTGGGGATC CTGGCAAGAA GAAACAGCAC 
ATTTGTCACA TGCAAGGCTG TGGCAAAGTA TATGGCAAGA CCTCACACCT ACGGGCACAC 
TTGCGCTGGC ATACGGGCGA GAGGCCTTTC ATGTGTAACT GGTCATTCTG TGGGAAACGC 
TTCACACGTT CAGATGAGTT ACAGAGGCAC AAACGCACAC ACACAGGTGA GAAGAAATTT 
GCCTGCCCTG AGTGTCCGAA GCGCTTCATG AGGAGTGACC ACCTGTCAAA GCATATCAAG 
ACCCACCAGA ATAAGAAGGG AGGCCCGGGT GTAGCCCTGA GTGTGGGCAC TTTGCCCCTG 
GACAGTGGGG CAGGTTCAGA AGGCAGTGGT ACTGCCACCC CTTCAGCCCT TATTACCACC 
AACATGGTAG CCATGGAGGC CATTTGTCCG GAGGGTATTG CCCGTCTTGC CAACAGTGGC 
ATCAATGTCA TGCAGGTGGC AGATCTGCAG TCCATTAATA TCAGTGGCAA TGGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SP1 ( XM_025284255.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025140040.1 Bubalus bubalis Sp1 transcription factor (SP1), transcript variant X2, mRNA.

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CloneID	OBu06068
Clone ID Related Accession (Same CDS sequence)	XM_006047465.1 , XM_025284255.1
Accession Version	XM_025284255.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	transcription factor Sp1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAA TGACAGCAGT GGTGAAGATT GAAAAAGGAG TTGGTGGCAG TAACGGGGGC 
AATGGAAATG GTAGTGGTGC CTTTTCTCAG GCTCGAAGCA GCAGCGCAGG CAGCAGCAGC 
AGCAGCGGAG GAGGAGGGCA GGAATCCCAG CCATCCCCTT TGGCTCTGCT GGCAGCAACT 
TGCAGCAGAA TTGAGTCACC CAATGAAAAC AGCAACAACT CCCAGGGCCC AAGCCAGTCA 
GGGGGCACAG GTGAGCTTGA CCTCACAGCC ACACAACTTT CCCAGGGTGC CAATGGCTGG 
CAGATCATCT CTTCCTCCTC TGGGGCTACC CCTACCTCGA AGGAACAGAG TGGCAGCAGT 
ACCAACGGCA ACAATGGCAG TGAGTCTTCC AAGAATCGCA CGGTCTCCGG TGGGCAGTAT 
GTTGTGGCTG CCACTTCCAA CCTTCAGAAC CAGCAAGTCC TGACCGGACT ACCTGGAGTG 
ATGCCTAATA TTCAGTATCA AGTAATCCCA CAGTTCCAGA CCGTTGATGG GCAACAGCTG 
CAGTTTGCTG CCACTGGGGC CCAAGTGCAG CAGGATGGTT CAGGTCAGAT ACAGATCATA 
CCAGGTGCAA ACCAACAGAT CATCACAAAC CGAGGAAGTG GAGGCAACAT CATTGCTGCT 
ATGCCAAACC TACTCCAGCA GGCTGTCCCC CTTCAAGGCC TGGCTAATAA TGTACTCTCA 
GGACAGACTC AGTATGTGAC CAACGTACCA GTGGCCCTGA ATGGGAATAT TACCTTGCTG 
CCTGTCAACA GCGTTTCTGC AGCTACCTTG ACTCCTAGCT CTCAGGCAGT CACGATCAGC 
AGCTCTGGGT CCCAGGAGAG TGGCTCACAG CCTGTCACCT CAGGGACTGC CATCAGTTCT 
GCCAGTTTGG TATCTTCACA AGCCAGTTCC AGCTCCTTTT TCACCAATGC CAATAGCTAC 
TCAACAACTA CTACCACCAG CAACATGGGA ATTATGAACT TTACCACCAG CGGATCAGCA 
GGGACCAGCT CTCAGAGCCA GACACCCCAG AGGGTCAGCG GGCTGCAGGG ATCTGATACT 
CTGAACATCC AGCAGAACCA GACATCTGGA GGTTCACTGC AAGCAAGTCA ACAAAAAGAG 
GGAGAGCAAA ACCAGCAGAC ACAGCAACAA CAACAAATTC TCATCCAGCC TCAGCTGGTT 
CAAGGGGGAC AGGCCCTTCA GGCCCTCCAA GCCACCCCAT TGTCAGGGCA GACCTTTACA 
ACTCAAGCTA TCACCCAGGA AACCCTCCAG AACCTTCAGC TTCAGGCTGT TCCAAACTCT 
GGCCCCATCA TCATCCGGAC ACCAACAGTG GGGCCCAATG GACAGGTCAG CTGGCAGACT 
CTTCAGCTGC AGAACCTCCA AGTTCAGAAC CCACAAGCCC AGACAATCAC CTTGGCCCCA 
ATGCAGGGTG TTTCTTTGGG GCAGACCAGC AGCAGCAGTA CCACCCTTAC GCCTATTGCC 
TCAGCTGCCT CCATCCCTGC CGGCACAGTC ACTGTGAATG CTGCTCAGCT CTCCTCCATG 
CCTGGCCTCC AGACCATTAA CCTCAGTGCA TTGGGTGCTC CAGGAATCCA GGTGCACCAG 
CTTCCAAGCC TACCCTTGGC AATAGCAAAT GCCTCAGGTG ATCATGGAGC TCAGCTTGGC 
CTCCATGGGG CTGGTGGTGA TGGAATACAT GATGACCCAA CAGGGGGAGA AGAAGGAGAG 
AACAGCCCAG ATCCCCAACC CCAAGCTGGT CGGAGGACCC GACGGGAAGC ATGCACCTGC 
CCCTACTGTA AAGACAGTGA AGGAAGGGGC TCTGGGGATC CTGGCAAGAA GAAACAGCAC 
ATTTGTCACA TGCAAGGCTG TGGCAAAGTA TATGGCAAGA CCTCACACCT ACGGGCACAC 
TTGCGCTGGC ATACGGGCGA GAGGCCTTTC ATGTGTAACT GGTCATTCTG TGGGAAACGC 
TTCACACGTT CAGATGAGTT ACAGAGGCAC AAACGCACAC ACACAGGTGA GAAGAAATTT 
GCCTGCCCTG AGTGTCCGAA GCGCTTCATG AGGAGTGACC ACCTGTCAAA GCATATCAAG 
ACCCACCAGA ATAAGAAGGG AGGCCCGGGT GTAGCCCTGA GTGTGGGCAC TTTGCCCCTG 
GACAGTGGGG CAGGTTCAGA AGGCAGTGGT ACTGCCACCC CTTCAGCCCT TATTACCACC 
AACATGGTAG CCATGGAGGC CATTTGTCCG GAGGGTATTG CCCGTCTTGC CAACAGTGGC 
ATCAATGTCA TGCAGGTGGC AGATCTGCAG TCCATTAATA TCAGTGGCAA TGGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025140040.1
CDS	452..2791
Translation	MDEMTAVVKIEKGVGGSNGGNGNGSGAFSQARSSSAGSSSSSGGGGQESQPSPLALLAATCSRIESPNENSNNSQGPSQSGGTGELDLTATQLSQGANGWQIISSSSGATPTSKEQSGSSTNGNNGSESSKNRTVSGGQYVVAATSNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQDGSGQIQIIPGANQQIITNRGSGGNIIAAMPNLLQQAVPLQGLANNVLSGQTQYVTNVPVALNGNITLLPVNSVSAATLTPSSQAVTISSSGSQESGSQPVTSGTAISSASLVSSQASSSSFFTNANSYSTTTTTSNMGIMNFTTSGSAGTSSQSQTPQRVSGLQGSDTLNIQQNQTSGGSLQASQQKEGEQNQQTQQQQQILIQPQLVQGGQALQALQATPLSGQTFTTQAITQETLQNLQLQAVPNSGPIIIRTPTVGPNGQVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQTSSSSTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGAPGIQVHQLPSLPLAIANASGDHGAQLGLHGAGGDGIHDDPTGGEEGENSPDPQPQAGRRTRREACTCPYCKDSEGRGSGDPGKKKQHICHMQGCGKVYGKTSHLRAHLRWHTGERPFMCNWSFCGKRFTRSDELQRHKRTHTGEKKFACPECPKRFMRSDHLSKHIKTHQNKKGGPGVALSVGTLPLDSGAGSEGSGTATPSALITTNMVAMEAICPEGIARLANSGINVMQVADLQSINISGNGF

Target ORF information:

RefSeq Version	XM_025284255.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis Sp1 transcription factor (SP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025284255.1

ORF Insert Sequence:

ATGGATGAAA TGACAGCAGT GGTGAAGATT GAAAAAGGAG TTGGTGGCAG TAACGGGGGC 
AATGGAAATG GTAGTGGTGC CTTTTCTCAG GCTCGAAGCA GCAGCGCAGG CAGCAGCAGC 
AGCAGCGGAG GAGGAGGGCA GGAATCCCAG CCATCCCCTT TGGCTCTGCT GGCAGCAACT 
TGCAGCAGAA TTGAGTCACC CAATGAAAAC AGCAACAACT CCCAGGGCCC AAGCCAGTCA 
GGGGGCACAG GTGAGCTTGA CCTCACAGCC ACACAACTTT CCCAGGGTGC CAATGGCTGG 
CAGATCATCT CTTCCTCCTC TGGGGCTACC CCTACCTCGA AGGAACAGAG TGGCAGCAGT 
ACCAACGGCA ACAATGGCAG TGAGTCTTCC AAGAATCGCA CGGTCTCCGG TGGGCAGTAT 
GTTGTGGCTG CCACTTCCAA CCTTCAGAAC CAGCAAGTCC TGACCGGACT ACCTGGAGTG 
ATGCCTAATA TTCAGTATCA AGTAATCCCA CAGTTCCAGA CCGTTGATGG GCAACAGCTG 
CAGTTTGCTG CCACTGGGGC CCAAGTGCAG CAGGATGGTT CAGGTCAGAT ACAGATCATA 
CCAGGTGCAA ACCAACAGAT CATCACAAAC CGAGGAAGTG GAGGCAACAT CATTGCTGCT 
ATGCCAAACC TACTCCAGCA GGCTGTCCCC CTTCAAGGCC TGGCTAATAA TGTACTCTCA 
GGACAGACTC AGTATGTGAC CAACGTACCA GTGGCCCTGA ATGGGAATAT TACCTTGCTG 
CCTGTCAACA GCGTTTCTGC AGCTACCTTG ACTCCTAGCT CTCAGGCAGT CACGATCAGC 
AGCTCTGGGT CCCAGGAGAG TGGCTCACAG CCTGTCACCT CAGGGACTGC CATCAGTTCT 
GCCAGTTTGG TATCTTCACA AGCCAGTTCC AGCTCCTTTT TCACCAATGC CAATAGCTAC 
TCAACAACTA CTACCACCAG CAACATGGGA ATTATGAACT TTACCACCAG CGGATCAGCA 
GGGACCAGCT CTCAGAGCCA GACACCCCAG AGGGTCAGCG GGCTGCAGGG ATCTGATACT 
CTGAACATCC AGCAGAACCA GACATCTGGA GGTTCACTGC AAGCAAGTCA ACAAAAAGAG 
GGAGAGCAAA ACCAGCAGAC ACAGCAACAA CAACAAATTC TCATCCAGCC TCAGCTGGTT 
CAAGGGGGAC AGGCCCTTCA GGCCCTCCAA GCCACCCCAT TGTCAGGGCA GACCTTTACA 
ACTCAAGCTA TCACCCAGGA AACCCTCCAG AACCTTCAGC TTCAGGCTGT TCCAAACTCT 
GGCCCCATCA TCATCCGGAC ACCAACAGTG GGGCCCAATG GACAGGTCAG CTGGCAGACT 
CTTCAGCTGC AGAACCTCCA AGTTCAGAAC CCACAAGCCC AGACAATCAC CTTGGCCCCA 
ATGCAGGGTG TTTCTTTGGG GCAGACCAGC AGCAGCAGTA CCACCCTTAC GCCTATTGCC 
TCAGCTGCCT CCATCCCTGC CGGCACAGTC ACTGTGAATG CTGCTCAGCT CTCCTCCATG 
CCTGGCCTCC AGACCATTAA CCTCAGTGCA TTGGGTGCTC CAGGAATCCA GGTGCACCAG 
CTTCCAAGCC TACCCTTGGC AATAGCAAAT GCCTCAGGTG ATCATGGAGC TCAGCTTGGC 
CTCCATGGGG CTGGTGGTGA TGGAATACAT GATGACCCAA CAGGGGGAGA AGAAGGAGAG 
AACAGCCCAG ATCCCCAACC CCAAGCTGGT CGGAGGACCC GACGGGAAGC ATGCACCTGC 
CCCTACTGTA AAGACAGTGA AGGAAGGGGC TCTGGGGATC CTGGCAAGAA GAAACAGCAC 
ATTTGTCACA TGCAAGGCTG TGGCAAAGTA TATGGCAAGA CCTCACACCT ACGGGCACAC 
TTGCGCTGGC ATACGGGCGA GAGGCCTTTC ATGTGTAACT GGTCATTCTG TGGGAAACGC 
TTCACACGTT CAGATGAGTT ACAGAGGCAC AAACGCACAC ACACAGGTGA GAAGAAATTT 
GCCTGCCCTG AGTGTCCGAA GCGCTTCATG AGGAGTGACC ACCTGTCAAA GCATATCAAG 
ACCCACCAGA ATAAGAAGGG AGGCCCGGGT GTAGCCCTGA GTGTGGGCAC TTTGCCCCTG 
GACAGTGGGG CAGGTTCAGA AGGCAGTGGT ACTGCCACCC CTTCAGCCCT TATTACCACC 
AACATGGTAG CCATGGAGGC CATTTGTCCG GAGGGTATTG CCCGTCTTGC CAACAGTGGC 
ATCAATGTCA TGCAGGTGGC AGATCTGCAG TCCATTAATA TCAGTGGCAA TGGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SP1 ( XM_025284256.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025140041.1 Bubalus bubalis Sp1 transcription factor (SP1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu06069
Clone ID Related Accession (Same CDS sequence)	XM_025284256.1 , XM_006047466.1
Accession Version	XM_025284256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	transcription factor Sp1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGACC AAGATCACTC CATGGATGAA ATGACAGCAG TGGTGAAGAT TGAAAAAGGA 
GTTGGTGGCA GTAACGGGGG CAATGGAAAT GGTAGTGGTG CCTTTTCTCA GGCTCGAAGC 
AGCAGCGCAG GCAGCAGCAG CAGCAGCGGA GGAGGAGGGC AGGGTGCCAA TGGCTGGCAG 
ATCATCTCTT CCTCCTCTGG GGCTACCCCT ACCTCGAAGG AACAGAGTGG CAGCAGTACC 
AACGGCAACA ATGGCAGTGA GTCTTCCAAG AATCGCACGG TCTCCGGTGG GCAGTATGTT 
GTGGCTGCCA CTTCCAACCT TCAGAACCAG CAAGTCCTGA CCGGACTACC TGGAGTGATG 
CCTAATATTC AGTATCAAGT AATCCCACAG TTCCAGACCG TTGATGGGCA ACAGCTGCAG 
TTTGCTGCCA CTGGGGCCCA AGTGCAGCAG GATGGTTCAG GTCAGATACA GATCATACCA 
GGTGCAAACC AACAGATCAT CACAAACCGA GGAAGTGGAG GCAACATCAT TGCTGCTATG 
CCAAACCTAC TCCAGCAGGC TGTCCCCCTT CAAGGCCTGG CTAATAATGT ACTCTCAGGA 
CAGACTCAGT ATGTGACCAA CGTACCAGTG GCCCTGAATG GGAATATTAC CTTGCTGCCT 
GTCAACAGCG TTTCTGCAGC TACCTTGACT CCTAGCTCTC AGGCAGTCAC GATCAGCAGC 
TCTGGGTCCC AGGAGAGTGG CTCACAGCCT GTCACCTCAG GGACTGCCAT CAGTTCTGCC 
AGTTTGGTAT CTTCACAAGC CAGTTCCAGC TCCTTTTTCA CCAATGCCAA TAGCTACTCA 
ACAACTACTA CCACCAGCAA CATGGGAATT ATGAACTTTA CCACCAGCGG ATCAGCAGGG 
ACCAGCTCTC AGAGCCAGAC ACCCCAGAGG GTCAGCGGGC TGCAGGGATC TGATACTCTG 
AACATCCAGC AGAACCAGAC ATCTGGAGGT TCACTGCAAG CAAGTCAACA AAAAGAGGGA 
GAGCAAAACC AGCAGACACA GCAACAACAA CAAATTCTCA TCCAGCCTCA GCTGGTTCAA 
GGGGGACAGG CCCTTCAGGC CCTCCAAGCC ACCCCATTGT CAGGGCAGAC CTTTACAACT 
CAAGCTATCA CCCAGGAAAC CCTCCAGAAC CTTCAGCTTC AGGCTGTTCC AAACTCTGGC 
CCCATCATCA TCCGGACACC AACAGTGGGG CCCAATGGAC AGGTCAGCTG GCAGACTCTT 
CAGCTGCAGA ACCTCCAAGT TCAGAACCCA CAAGCCCAGA CAATCACCTT GGCCCCAATG 
CAGGGTGTTT CTTTGGGGCA GACCAGCAGC AGCAGTACCA CCCTTACGCC TATTGCCTCA 
GCTGCCTCCA TCCCTGCCGG CACAGTCACT GTGAATGCTG CTCAGCTCTC CTCCATGCCT 
GGCCTCCAGA CCATTAACCT CAGTGCATTG GGTGCTCCAG GAATCCAGGT GCACCAGCTT 
CCAAGCCTAC CCTTGGCAAT AGCAAATGCC TCAGGTGATC ATGGAGCTCA GCTTGGCCTC 
CATGGGGCTG GTGGTGATGG AATACATGAT GACCCAACAG GGGGAGAAGA AGGAGAGAAC 
AGCCCAGATC CCCAACCCCA AGCTGGTCGG AGGACCCGAC GGGAAGCATG CACCTGCCCC 
TACTGTAAAG ACAGTGAAGG AAGGGGCTCT GGGGATCCTG GCAAGAAGAA ACAGCACATT 
TGTCACATGC AAGGCTGTGG CAAAGTATAT GGCAAGACCT CACACCTACG GGCACACTTG 
CGCTGGCATA CGGGCGAGAG GCCTTTCATG TGTAACTGGT CATTCTGTGG GAAACGCTTC 
ACACGTTCAG ATGAGTTACA GAGGCACAAA CGCACACACA CAGGTGAGAA GAAATTTGCC 
TGCCCTGAGT GTCCGAAGCG CTTCATGAGG AGTGACCACC TGTCAAAGCA TATCAAGACC 
CACCAGAATA AGAAGGGAGG CCCGGGTGTA GCCCTGAGTG TGGGCACTTT GCCCCTGGAC 
AGTGGGGCAG GTTCAGAAGG CAGTGGTACT GCCACCCCTT CAGCCCTTAT TACCACCAAC 
ATGGTAGCCA TGGAGGCCAT TTGTCCGGAG GGTATTGCCC GTCTTGCCAA CAGTGGCATC 
AATGTCATGC AGGTGGCAGA TCTGCAGTCC ATTAATATCA GTGGCAATGG CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025140041.1
CDS	113..2329
Translation	MSDQDHSMDEMTAVVKIEKGVGGSNGGNGNGSGAFSQARSSSAGSSSSSGGGGQGANGWQIISSSSGATPTSKEQSGSSTNGNNGSESSKNRTVSGGQYVVAATSNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQDGSGQIQIIPGANQQIITNRGSGGNIIAAMPNLLQQAVPLQGLANNVLSGQTQYVTNVPVALNGNITLLPVNSVSAATLTPSSQAVTISSSGSQESGSQPVTSGTAISSASLVSSQASSSSFFTNANSYSTTTTTSNMGIMNFTTSGSAGTSSQSQTPQRVSGLQGSDTLNIQQNQTSGGSLQASQQKEGEQNQQTQQQQQILIQPQLVQGGQALQALQATPLSGQTFTTQAITQETLQNLQLQAVPNSGPIIIRTPTVGPNGQVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQTSSSSTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGAPGIQVHQLPSLPLAIANASGDHGAQLGLHGAGGDGIHDDPTGGEEGENSPDPQPQAGRRTRREACTCPYCKDSEGRGSGDPGKKKQHICHMQGCGKVYGKTSHLRAHLRWHTGERPFMCNWSFCGKRFTRSDELQRHKRTHTGEKKFACPECPKRFMRSDHLSKHIKTHQNKKGGPGVALSVGTLPLDSGAGSEGSGTATPSALITTNMVAMEAICPEGIARLANSGINVMQVADLQSINISGNGF

Target ORF information:

RefSeq Version	XM_025284256.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis Sp1 transcription factor (SP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025284256.1

ORF Insert Sequence:

ATGAGCGACC AAGATCACTC CATGGATGAA ATGACAGCAG TGGTGAAGAT TGAAAAAGGA 
GTTGGTGGCA GTAACGGGGG CAATGGAAAT GGTAGTGGTG CCTTTTCTCA GGCTCGAAGC 
AGCAGCGCAG GCAGCAGCAG CAGCAGCGGA GGAGGAGGGC AGGGTGCCAA TGGCTGGCAG 
ATCATCTCTT CCTCCTCTGG GGCTACCCCT ACCTCGAAGG AACAGAGTGG CAGCAGTACC 
AACGGCAACA ATGGCAGTGA GTCTTCCAAG AATCGCACGG TCTCCGGTGG GCAGTATGTT 
GTGGCTGCCA CTTCCAACCT TCAGAACCAG CAAGTCCTGA CCGGACTACC TGGAGTGATG 
CCTAATATTC AGTATCAAGT AATCCCACAG TTCCAGACCG TTGATGGGCA ACAGCTGCAG 
TTTGCTGCCA CTGGGGCCCA AGTGCAGCAG GATGGTTCAG GTCAGATACA GATCATACCA 
GGTGCAAACC AACAGATCAT CACAAACCGA GGAAGTGGAG GCAACATCAT TGCTGCTATG 
CCAAACCTAC TCCAGCAGGC TGTCCCCCTT CAAGGCCTGG CTAATAATGT ACTCTCAGGA 
CAGACTCAGT ATGTGACCAA CGTACCAGTG GCCCTGAATG GGAATATTAC CTTGCTGCCT 
GTCAACAGCG TTTCTGCAGC TACCTTGACT CCTAGCTCTC AGGCAGTCAC GATCAGCAGC 
TCTGGGTCCC AGGAGAGTGG CTCACAGCCT GTCACCTCAG GGACTGCCAT CAGTTCTGCC 
AGTTTGGTAT CTTCACAAGC CAGTTCCAGC TCCTTTTTCA CCAATGCCAA TAGCTACTCA 
ACAACTACTA CCACCAGCAA CATGGGAATT ATGAACTTTA CCACCAGCGG ATCAGCAGGG 
ACCAGCTCTC AGAGCCAGAC ACCCCAGAGG GTCAGCGGGC TGCAGGGATC TGATACTCTG 
AACATCCAGC AGAACCAGAC ATCTGGAGGT TCACTGCAAG CAAGTCAACA AAAAGAGGGA 
GAGCAAAACC AGCAGACACA GCAACAACAA CAAATTCTCA TCCAGCCTCA GCTGGTTCAA 
GGGGGACAGG CCCTTCAGGC CCTCCAAGCC ACCCCATTGT CAGGGCAGAC CTTTACAACT 
CAAGCTATCA CCCAGGAAAC CCTCCAGAAC CTTCAGCTTC AGGCTGTTCC AAACTCTGGC 
CCCATCATCA TCCGGACACC AACAGTGGGG CCCAATGGAC AGGTCAGCTG GCAGACTCTT 
CAGCTGCAGA ACCTCCAAGT TCAGAACCCA CAAGCCCAGA CAATCACCTT GGCCCCAATG 
CAGGGTGTTT CTTTGGGGCA GACCAGCAGC AGCAGTACCA CCCTTACGCC TATTGCCTCA 
GCTGCCTCCA TCCCTGCCGG CACAGTCACT GTGAATGCTG CTCAGCTCTC CTCCATGCCT 
GGCCTCCAGA CCATTAACCT CAGTGCATTG GGTGCTCCAG GAATCCAGGT GCACCAGCTT 
CCAAGCCTAC CCTTGGCAAT AGCAAATGCC TCAGGTGATC ATGGAGCTCA GCTTGGCCTC 
CATGGGGCTG GTGGTGATGG AATACATGAT GACCCAACAG GGGGAGAAGA AGGAGAGAAC 
AGCCCAGATC CCCAACCCCA AGCTGGTCGG AGGACCCGAC GGGAAGCATG CACCTGCCCC 
TACTGTAAAG ACAGTGAAGG AAGGGGCTCT GGGGATCCTG GCAAGAAGAA ACAGCACATT 
TGTCACATGC AAGGCTGTGG CAAAGTATAT GGCAAGACCT CACACCTACG GGCACACTTG 
CGCTGGCATA CGGGCGAGAG GCCTTTCATG TGTAACTGGT CATTCTGTGG GAAACGCTTC 
ACACGTTCAG ATGAGTTACA GAGGCACAAA CGCACACACA CAGGTGAGAA GAAATTTGCC 
TGCCCTGAGT GTCCGAAGCG CTTCATGAGG AGTGACCACC TGTCAAAGCA TATCAAGACC 
CACCAGAATA AGAAGGGAGG CCCGGGTGTA GCCCTGAGTG TGGGCACTTT GCCCCTGGAC 
AGTGGGGCAG GTTCAGAAGG CAGTGGTACT GCCACCCCTT CAGCCCTTAT TACCACCAAC 
ATGGTAGCCA TGGAGGCCAT TTGTCCGGAG GGTATTGCCC GTCTTGCCAA CAGTGGCATC 
AATGTCATGC AGGTGGCAGA TCTGCAGTCC ATTAATATCA GTGGCAATGG CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SP1 ( XM_006047466.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047528.1 Bubalus bubalis Sp1 transcription factor (SP1), transcript variant X3, mRNA.

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CloneID	OBu06069
Clone ID Related Accession (Same CDS sequence)	XM_025284256.1 , XM_006047466.1
Accession Version	XM_006047466.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	transcription factor Sp1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784022.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGACC AAGATCACTC CATGGATGAA ATGACAGCAG TGGTGAAGAT TGAAAAAGGA 
GTTGGTGGCA GTAACGGGGG CAATGGAAAT GGTAGTGGTG CCTTTTCTCA GGCTCGAAGC 
AGCAGCGCAG GCAGCAGCAG CAGCAGCGGA GGAGGAGGGC AGGGTGCCAA TGGCTGGCAG 
ATCATCTCTT CCTCCTCTGG GGCTACCCCT ACCTCGAAGG AACAGAGTGG CAGCAGTACC 
AACGGCAACA ATGGCAGTGA GTCTTCCAAG AATCGCACGG TCTCCGGTGG GCAGTATGTT 
GTGGCTGCCA CTTCCAACCT TCAGAACCAG CAAGTCCTGA CCGGACTACC TGGAGTGATG 
CCTAATATTC AGTATCAAGT AATCCCACAG TTCCAGACCG TTGATGGGCA ACAGCTGCAG 
TTTGCTGCCA CTGGGGCCCA AGTGCAGCAG GATGGTTCAG GTCAGATACA GATCATACCA 
GGTGCAAACC AACAGATCAT CACAAACCGA GGAAGTGGAG GCAACATCAT TGCTGCTATG 
CCAAACCTAC TCCAGCAGGC TGTCCCCCTT CAAGGCCTGG CTAATAATGT ACTCTCAGGA 
CAGACTCAGT ATGTGACCAA CGTACCAGTG GCCCTGAATG GGAATATTAC CTTGCTGCCT 
GTCAACAGCG TTTCTGCAGC TACCTTGACT CCTAGCTCTC AGGCAGTCAC GATCAGCAGC 
TCTGGGTCCC AGGAGAGTGG CTCACAGCCT GTCACCTCAG GGACTGCCAT CAGTTCTGCC 
AGTTTGGTAT CTTCACAAGC CAGTTCCAGC TCCTTTTTCA CCAATGCCAA TAGCTACTCA 
ACAACTACTA CCACCAGCAA CATGGGAATT ATGAACTTTA CCACCAGCGG ATCAGCAGGG 
ACCAGCTCTC AGAGCCAGAC ACCCCAGAGG GTCAGCGGGC TGCAGGGATC TGATACTCTG 
AACATCCAGC AGAACCAGAC ATCTGGAGGT TCACTGCAAG CAAGTCAACA AAAAGAGGGA 
GAGCAAAACC AGCAGACACA GCAACAACAA CAAATTCTCA TCCAGCCTCA GCTGGTTCAA 
GGGGGACAGG CCCTTCAGGC CCTCCAAGCC ACCCCATTGT CAGGGCAGAC CTTTACAACT 
CAAGCTATCA CCCAGGAAAC CCTCCAGAAC CTTCAGCTTC AGGCTGTTCC AAACTCTGGC 
CCCATCATCA TCCGGACACC AACAGTGGGG CCCAATGGAC AGGTCAGCTG GCAGACTCTT 
CAGCTGCAGA ACCTCCAAGT TCAGAACCCA CAAGCCCAGA CAATCACCTT GGCCCCAATG 
CAGGGTGTTT CTTTGGGGCA GACCAGCAGC AGCAGTACCA CCCTTACGCC TATTGCCTCA 
GCTGCCTCCA TCCCTGCCGG CACAGTCACT GTGAATGCTG CTCAGCTCTC CTCCATGCCT 
GGCCTCCAGA CCATTAACCT CAGTGCATTG GGTGCTCCAG GAATCCAGGT GCACCAGCTT 
CCAAGCCTAC CCTTGGCAAT AGCAAATGCC TCAGGTGATC ATGGAGCTCA GCTTGGCCTC 
CATGGGGCTG GTGGTGATGG AATACATGAT GACCCAACAG GGGGAGAAGA AGGAGAGAAC 
AGCCCAGATC CCCAACCCCA AGCTGGTCGG AGGACCCGAC GGGAAGCATG CACCTGCCCC 
TACTGTAAAG ACAGTGAAGG AAGGGGCTCT GGGGATCCTG GCAAGAAGAA ACAGCACATT 
TGTCACATGC AAGGCTGTGG CAAAGTATAT GGCAAGACCT CACACCTACG GGCACACTTG 
CGCTGGCATA CGGGCGAGAG GCCTTTCATG TGTAACTGGT CATTCTGTGG GAAACGCTTC 
ACACGTTCAG ATGAGTTACA GAGGCACAAA CGCACACACA CAGGTGAGAA GAAATTTGCC 
TGCCCTGAGT GTCCGAAGCG CTTCATGAGG AGTGACCACC TGTCAAAGCA TATCAAGACC 
CACCAGAATA AGAAGGGAGG CCCGGGTGTA GCCCTGAGTG TGGGCACTTT GCCCCTGGAC 
AGTGGGGCAG GTTCAGAAGG CAGTGGTACT GCCACCCCTT CAGCCCTTAT TACCACCAAC 
ATGGTAGCCA TGGAGGCCAT TTGTCCGGAG GGTATTGCCC GTCTTGCCAA CAGTGGCATC 
AATGTCATGC AGGTGGCAGA TCTGCAGTCC ATTAATATCA GTGGCAATGG CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047528.1
CDS	83..2299
Translation	MSDQDHSMDEMTAVVKIEKGVGGSNGGNGNGSGAFSQARSSSAGSSSSSGGGGQGANGWQIISSSSGATPTSKEQSGSSTNGNNGSESSKNRTVSGGQYVVAATSNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQDGSGQIQIIPGANQQIITNRGSGGNIIAAMPNLLQQAVPLQGLANNVLSGQTQYVTNVPVALNGNITLLPVNSVSAATLTPSSQAVTISSSGSQESGSQPVTSGTAISSASLVSSQASSSSFFTNANSYSTTTTTSNMGIMNFTTSGSAGTSSQSQTPQRVSGLQGSDTLNIQQNQTSGGSLQASQQKEGEQNQQTQQQQQILIQPQLVQGGQALQALQATPLSGQTFTTQAITQETLQNLQLQAVPNSGPIIIRTPTVGPNGQVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQTSSSSTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGAPGIQVHQLPSLPLAIANASGDHGAQLGLHGAGGDGIHDDPTGGEEGENSPDPQPQAGRRTRREACTCPYCKDSEGRGSGDPGKKKQHICHMQGCGKVYGKTSHLRAHLRWHTGERPFMCNWSFCGKRFTRSDELQRHKRTHTGEKKFACPECPKRFMRSDHLSKHIKTHQNKKGGPGVALSVGTLPLDSGAGSEGSGTATPSALITTNMVAMEAICPEGIARLANSGINVMQVADLQSINISGNGF

Target ORF information:

RefSeq Version	XM_006047466.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis Sp1 transcription factor (SP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047466.1

ORF Insert Sequence:

ATGAGCGACC AAGATCACTC CATGGATGAA ATGACAGCAG TGGTGAAGAT TGAAAAAGGA 
GTTGGTGGCA GTAACGGGGG CAATGGAAAT GGTAGTGGTG CCTTTTCTCA GGCTCGAAGC 
AGCAGCGCAG GCAGCAGCAG CAGCAGCGGA GGAGGAGGGC AGGGTGCCAA TGGCTGGCAG 
ATCATCTCTT CCTCCTCTGG GGCTACCCCT ACCTCGAAGG AACAGAGTGG CAGCAGTACC 
AACGGCAACA ATGGCAGTGA GTCTTCCAAG AATCGCACGG TCTCCGGTGG GCAGTATGTT 
GTGGCTGCCA CTTCCAACCT TCAGAACCAG CAAGTCCTGA CCGGACTACC TGGAGTGATG 
CCTAATATTC AGTATCAAGT AATCCCACAG TTCCAGACCG TTGATGGGCA ACAGCTGCAG 
TTTGCTGCCA CTGGGGCCCA AGTGCAGCAG GATGGTTCAG GTCAGATACA GATCATACCA 
GGTGCAAACC AACAGATCAT CACAAACCGA GGAAGTGGAG GCAACATCAT TGCTGCTATG 
CCAAACCTAC TCCAGCAGGC TGTCCCCCTT CAAGGCCTGG CTAATAATGT ACTCTCAGGA 
CAGACTCAGT ATGTGACCAA CGTACCAGTG GCCCTGAATG GGAATATTAC CTTGCTGCCT 
GTCAACAGCG TTTCTGCAGC TACCTTGACT CCTAGCTCTC AGGCAGTCAC GATCAGCAGC 
TCTGGGTCCC AGGAGAGTGG CTCACAGCCT GTCACCTCAG GGACTGCCAT CAGTTCTGCC 
AGTTTGGTAT CTTCACAAGC CAGTTCCAGC TCCTTTTTCA CCAATGCCAA TAGCTACTCA 
ACAACTACTA CCACCAGCAA CATGGGAATT ATGAACTTTA CCACCAGCGG ATCAGCAGGG 
ACCAGCTCTC AGAGCCAGAC ACCCCAGAGG GTCAGCGGGC TGCAGGGATC TGATACTCTG 
AACATCCAGC AGAACCAGAC ATCTGGAGGT TCACTGCAAG CAAGTCAACA AAAAGAGGGA 
GAGCAAAACC AGCAGACACA GCAACAACAA CAAATTCTCA TCCAGCCTCA GCTGGTTCAA 
GGGGGACAGG CCCTTCAGGC CCTCCAAGCC ACCCCATTGT CAGGGCAGAC CTTTACAACT 
CAAGCTATCA CCCAGGAAAC CCTCCAGAAC CTTCAGCTTC AGGCTGTTCC AAACTCTGGC 
CCCATCATCA TCCGGACACC AACAGTGGGG CCCAATGGAC AGGTCAGCTG GCAGACTCTT 
CAGCTGCAGA ACCTCCAAGT TCAGAACCCA CAAGCCCAGA CAATCACCTT GGCCCCAATG 
CAGGGTGTTT CTTTGGGGCA GACCAGCAGC AGCAGTACCA CCCTTACGCC TATTGCCTCA 
GCTGCCTCCA TCCCTGCCGG CACAGTCACT GTGAATGCTG CTCAGCTCTC CTCCATGCCT 
GGCCTCCAGA CCATTAACCT CAGTGCATTG GGTGCTCCAG GAATCCAGGT GCACCAGCTT 
CCAAGCCTAC CCTTGGCAAT AGCAAATGCC TCAGGTGATC ATGGAGCTCA GCTTGGCCTC 
CATGGGGCTG GTGGTGATGG AATACATGAT GACCCAACAG GGGGAGAAGA AGGAGAGAAC 
AGCCCAGATC CCCAACCCCA AGCTGGTCGG AGGACCCGAC GGGAAGCATG CACCTGCCCC 
TACTGTAAAG ACAGTGAAGG AAGGGGCTCT GGGGATCCTG GCAAGAAGAA ACAGCACATT 
TGTCACATGC AAGGCTGTGG CAAAGTATAT GGCAAGACCT CACACCTACG GGCACACTTG 
CGCTGGCATA CGGGCGAGAG GCCTTTCATG TGTAACTGGT CATTCTGTGG GAAACGCTTC 
ACACGTTCAG ATGAGTTACA GAGGCACAAA CGCACACACA CAGGTGAGAA GAAATTTGCC 
TGCCCTGAGT GTCCGAAGCG CTTCATGAGG AGTGACCACC TGTCAAAGCA TATCAAGACC 
CACCAGAATA AGAAGGGAGG CCCGGGTGTA GCCCTGAGTG TGGGCACTTT GCCCCTGGAC 
AGTGGGGCAG GTTCAGAAGG CAGTGGTACT GCCACCCCTT CAGCCCTTAT TACCACCAAC 
ATGGTAGCCA TGGAGGCCAT TTGTCCGGAG GGTATTGCCC GTCTTGCCAA CAGTGGCATC 
AATGTCATGC AGGTGGCAGA TCTGCAGTCC ATTAATATCA GTGGCAATGG CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SP1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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