NISCH cDNA ORF clone, Bubalus bubalis(water buffalo)

The following NISCH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NISCH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu03273 XM_006044041.2
Latest version!
Bubalus bubalis nischarin (NISCH), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OBu57731 XM_025272431.1
Latest version!
Bubalus bubalis nischarin (NISCH), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu57730 XM_006044040.2
Latest version!
Bubalus bubalis nischarin (NISCH), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBu03272 XM_006044040.1
Latest version!
Bubalus bubalis nischarin (NISCH), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
View Old Accession Versions >>
OBu03273 XM_006044041.1 Bubalus bubalis nischarin (NISCH), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu03273
    Clone ID Related Accession (Same CDS sequence) XM_006044041.2 , XM_006044041.1
    Accession Version XM_006044041.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1551bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product nischarin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006044041.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCGGCGG CGGCGCGCAG CTTCGGGCCC GAGCGCGAGG CCGAGCCGGT CAAGACGGCG 
    CGCGTCGTGG GCTCTGAGCT CGTGGACACG TATACGGTTT ACATCATCCA GATCACTGAT
    GGGAGCCATG AGTGGACAGT CAAGCACCGC TACAGTGATT TCCACGACCT ACATGAAAAG
    CTTGTTGCGG AGAAGAAGAT TGATAGAAGT CTGCTTCCAC CCAAAAAGAT AATTGGGAAA
    AACTCCAGGA GCTTGGTGGA AAAGAGGGAG AAGGATCTCG AAATTTATCT CCAGACGCTC
    CTGGCCACCT TCCCTGACGT GGCCCCCAGA GTGCTGGCCC ACTTCTTGCA TTTCCACTTT
    TACGAGATTA ATGGCATCAC TGCAGCACTG GCTGAGGAAC TCTTCGAAAA AGGAGAACAG
    CTCCTGGGGG CTGGCGAGGT CTTCGCCATT GGACCCCTGC AGCTCTACGC AGTCACCGAG
    CAGCTGCAGC AGGGAAAGCC CACATGCGCC AGCGGGGATG CCAAGACTGA CCTGGGGCAC
    ATCCTGGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAAA AGGACCTTTT
    GGGACCAGCA ACATTCAGGA GCAGCTCCTG CCTTTTGATC TGTCCATATT CAAGTCTCTT
    CATCAGGTGG AGATAAGTCA CTGTGATGCC AGGCGCATCC GGGGGCTGGT TGCATCCAAG
    CCCACCTTGG CCACAATGAG CGTCCGTTTC TCGGCAACCT CCATGAAGGA AGTCCTCGTT
    CCTGAAGCCT CGGAGTTCGA TGAGTGGGAG CCAGCAGGCG CAGCCCTGGA GGGCCCCGTG
    ACTGCCGTCA TCCCCACATG GCAGGCTCTG ACCGCTCTGG ACTTGAGTCA CAATAGCATC
    TCTGAGATCG ATGATTCTGT GAAACTGATT CCAAAGATTG AGTTCCTGGA CCTGAGTCAC
    AATGGAGTGC TGGTCATGAA CAATTTGCAG CACCTGTACA ACCTTGTGCA CGTGGACCTG
    TCCTACAACA AGCTCTCCTC CTTGGAAGGG GTTCACACCA AACTGGGCAA CATCAAGACC
    CTGAACCTGG CAGGCAACCT CCTGAGGCAT CTGAGTGGCC TGCACAAGCT GTACTCCTTG
    GTCAACCTGG ATCTCAGCGA CAACAGAATC GAACAGATGG AAGAGGTCAG AAGCATCGGC
    AGCCTCCCGT GTCTGGAGCA CGTGGCTCTG CTGAACAACC CCCTGAGTAT CATTCCCGAC
    TACCGGACCA AGGTGCTGGC TCAGTTCGGA GAGCGGGCCT CGGAGGTCTG CCTGGACAAC
    ACAGTGACCA CAGAGAAAGA GTTGGACACA GTGGAAGTGC TAAAAGCGAT TCAGAAAGCC
    AAAGAGGTCA AGTCCAAATT AAGCAACCCT GAGAAGAAGG TCAGTGAGGA TTCTCGGCTC
    TCTGCTGCCC CCTGTGTCAG ACCCAGCAGC TCCCCACCCA GTGCGGCTCC CACCTCTGCC
    TCCCTGCCCC AGCCCATCCT CTCCAACCAA GGCATCCTCG GAGATGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006044103.1
    CDS116..1666
    Translation

    Target ORF information:

    RefSeq Version XM_006044041.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis nischarin (NISCH), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006044041.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCGGCGG CGGCGCGCAG CTTCGGGCCC GAGCGCGAGG CCGAGCCGGT CAAGACGGCG 
    CGCGTCGTGG GCTCTGAGCT CGTGGACACG TATACGGTTT ACATCATCCA GATCACTGAT
    GGGAGCCATG AGTGGACAGT CAAGCACCGC TACAGTGATT TCCACGACCT ACATGAAAAG
    CTTGTTGCGG AGAAGAAGAT TGATAGAAGT CTGCTTCCAC CCAAAAAGAT AATTGGGAAA
    AACTCCAGGA GCTTGGTGGA AAAGAGGGAG AAGGATCTCG AAATTTATCT CCAGACGCTC
    CTGGCCACCT TCCCTGACGT GGCCCCCAGA GTGCTGGCCC ACTTCTTGCA TTTCCACTTT
    TACGAGATTA ATGGCATCAC TGCAGCACTG GCTGAGGAAC TCTTCGAAAA AGGAGAACAG
    CTCCTGGGGG CTGGCGAGGT CTTCGCCATT GGACCCCTGC AGCTCTACGC AGTCACCGAG
    CAGCTGCAGC AGGGAAAGCC CACATGCGCC AGCGGGGATG CCAAGACTGA CCTGGGGCAC
    ATCCTGGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAAA AGGACCTTTT
    GGGACCAGCA ACATTCAGGA GCAGCTCCTG CCTTTTGATC TGTCCATATT CAAGTCTCTT
    CATCAGGTGG AGATAAGTCA CTGTGATGCC AGGCGCATCC GGGGGCTGGT TGCATCCAAG
    CCCACCTTGG CCACAATGAG CGTCCGTTTC TCGGCAACCT CCATGAAGGA AGTCCTCGTT
    CCTGAAGCCT CGGAGTTCGA TGAGTGGGAG CCAGCAGGCG CAGCCCTGGA GGGCCCCGTG
    ACTGCCGTCA TCCCCACATG GCAGGCTCTG ACCGCTCTGG ACTTGAGTCA CAATAGCATC
    TCTGAGATCG ATGATTCTGT GAAACTGATT CCAAAGATTG AGTTCCTGGA CCTGAGTCAC
    AATGGAGTGC TGGTCATGAA CAATTTGCAG CACCTGTACA ACCTTGTGCA CGTGGACCTG
    TCCTACAACA AGCTCTCCTC CTTGGAAGGG GTTCACACCA AACTGGGCAA CATCAAGACC
    CTGAACCTGG CAGGCAACCT CCTGAGGCAT CTGAGTGGCC TGCACAAGCT GTACTCCTTG
    GTCAACCTGG ATCTCAGCGA CAACAGAATC GAACAGATGG AAGAGGTCAG AAGCATCGGC
    AGCCTCCCGT GTCTGGAGCA CGTGGCTCTG CTGAACAACC CCCTGAGTAT CATTCCCGAC
    TACCGGACCA AGGTGCTGGC TCAGTTCGGA GAGCGGGCCT CGGAGGTCTG CCTGGACAAC
    ACAGTGACCA CAGAGAAAGA GTTGGACACA GTGGAAGTGC TAAAAGCGAT TCAGAAAGCC
    AAAGAGGTCA AGTCCAAATT AAGCAACCCT GAGAAGAAGG TCAGTGAGGA TTCTCGGCTC
    TCTGCTGCCC CCTGTGTCAG ACCCAGCAGC TCCCCACCCA GTGCGGCTCC CACCTCTGCC
    TCCCTGCCCC AGCCCATCCT CTCCAACCAA GGCATCCTCG GAGATGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu03273
    Clone ID Related Accession (Same CDS sequence) XM_006044041.2 , XM_006044041.1
    Accession Version XM_006044041.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1551bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product nischarin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783532.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCGGCGG CGGCGCGCAG CTTCGGGCCC GAGCGCGAGG CCGAGCCGGT CAAGACGGCG 
    CGCGTCGTGG GCTCTGAGCT CGTGGACACG TATACGGTTT ACATCATCCA GATCACTGAT
    GGGAGCCATG AGTGGACAGT CAAGCACCGC TACAGTGATT TCCACGACCT ACATGAAAAG
    CTTGTTGCGG AGAAGAAGAT TGATAGAAGT CTGCTTCCAC CCAAAAAGAT AATTGGGAAA
    AACTCCAGGA GCTTGGTGGA AAAGAGGGAG AAGGATCTCG AAATTTATCT CCAGACGCTC
    CTGGCCACCT TCCCTGACGT GGCCCCCAGA GTGCTGGCCC ACTTCTTGCA TTTCCACTTT
    TACGAGATTA ATGGCATCAC TGCAGCACTG GCTGAGGAAC TCTTCGAAAA AGGAGAACAG
    CTCCTGGGGG CTGGCGAGGT CTTCGCCATT GGACCCCTGC AGCTCTACGC AGTCACCGAG
    CAGCTGCAGC AGGGAAAGCC CACATGCGCC AGCGGGGATG CCAAGACTGA CCTGGGGCAC
    ATCCTGGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAAA AGGACCTTTT
    GGGACCAGCA ACATTCAGGA GCAGCTCCTG CCTTTTGATC TGTCCATATT CAAGTCTCTT
    CATCAGGTGG AGATAAGTCA CTGTGATGCC AGGCGCATCC GGGGGCTGGT TGCATCCAAG
    CCCACCTTGG CCACAATGAG CGTCCGTTTC TCGGCAACCT CCATGAAGGA AGTCCTCGTT
    CCTGAAGCCT CGGAGTTCGA TGAGTGGGAG CCAGCAGGCG CAGCCCTGGA GGGCCCCGTG
    ACTGCCGTCA TCCCCACATG GCAGGCTCTG ACCGCTCTGG ACTTGAGTCA CAATAGCATC
    TCTGAGATCG ATGATTCTGT GAAACTGATT CCAAAGATTG AGTTCCTGGA CCTGAGTCAC
    AATGGAGTGC TGGTCATGAA CAATTTGCAG CACCTGTACA ACCTTGTGCA CGTGGACCTG
    TCCTACAACA AGCTCTCCTC CTTGGAAGGG GTTCACACCA AACTGGGCAA CATCAAGACC
    CTGAACCTGG CAGGCAACCT CCTGAGGCAT CTGAGTGGCC TGCACAAGCT GTACTCCTTG
    GTCAACCTGG ATCTCAGCGA CAACAGAATC GAACAGATGG AAGAGGTCAG AAGCATCGGC
    AGCCTCCCGT GTCTGGAGCA CGTGGCTCTG CTGAACAACC CCCTGAGTAT CATTCCCGAC
    TACCGGACCA AGGTGCTGGC TCAGTTCGGA GAGCGGGCCT CGGAGGTCTG CCTGGACAAC
    ACAGTGACCA CAGAGAAAGA GTTGGACACA GTGGAAGTGC TAAAAGCGAT TCAGAAAGCC
    AAAGAGGTCA AGTCCAAATT AAGCAACCCT GAGAAGAAGG TCAGTGAGGA TTCTCGGCTC
    TCTGCTGCCC CCTGTGTCAG ACCCAGCAGC TCCCCACCCA GTGCGGCTCC CACCTCTGCC
    TCCCTGCCCC AGCCCATCCT CTCCAACCAA GGCATCCTCG GAGATGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006044103.1
    CDS72..1622
    Translation

    Target ORF information:

    RefSeq Version XM_006044041.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis nischarin (NISCH), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006044041.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCGGCGG CGGCGCGCAG CTTCGGGCCC GAGCGCGAGG CCGAGCCGGT CAAGACGGCG 
    CGCGTCGTGG GCTCTGAGCT CGTGGACACG TATACGGTTT ACATCATCCA GATCACTGAT
    GGGAGCCATG AGTGGACAGT CAAGCACCGC TACAGTGATT TCCACGACCT ACATGAAAAG
    CTTGTTGCGG AGAAGAAGAT TGATAGAAGT CTGCTTCCAC CCAAAAAGAT AATTGGGAAA
    AACTCCAGGA GCTTGGTGGA AAAGAGGGAG AAGGATCTCG AAATTTATCT CCAGACGCTC
    CTGGCCACCT TCCCTGACGT GGCCCCCAGA GTGCTGGCCC ACTTCTTGCA TTTCCACTTT
    TACGAGATTA ATGGCATCAC TGCAGCACTG GCTGAGGAAC TCTTCGAAAA AGGAGAACAG
    CTCCTGGGGG CTGGCGAGGT CTTCGCCATT GGACCCCTGC AGCTCTACGC AGTCACCGAG
    CAGCTGCAGC AGGGAAAGCC CACATGCGCC AGCGGGGATG CCAAGACTGA CCTGGGGCAC
    ATCCTGGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAAA AGGACCTTTT
    GGGACCAGCA ACATTCAGGA GCAGCTCCTG CCTTTTGATC TGTCCATATT CAAGTCTCTT
    CATCAGGTGG AGATAAGTCA CTGTGATGCC AGGCGCATCC GGGGGCTGGT TGCATCCAAG
    CCCACCTTGG CCACAATGAG CGTCCGTTTC TCGGCAACCT CCATGAAGGA AGTCCTCGTT
    CCTGAAGCCT CGGAGTTCGA TGAGTGGGAG CCAGCAGGCG CAGCCCTGGA GGGCCCCGTG
    ACTGCCGTCA TCCCCACATG GCAGGCTCTG ACCGCTCTGG ACTTGAGTCA CAATAGCATC
    TCTGAGATCG ATGATTCTGT GAAACTGATT CCAAAGATTG AGTTCCTGGA CCTGAGTCAC
    AATGGAGTGC TGGTCATGAA CAATTTGCAG CACCTGTACA ACCTTGTGCA CGTGGACCTG
    TCCTACAACA AGCTCTCCTC CTTGGAAGGG GTTCACACCA AACTGGGCAA CATCAAGACC
    CTGAACCTGG CAGGCAACCT CCTGAGGCAT CTGAGTGGCC TGCACAAGCT GTACTCCTTG
    GTCAACCTGG ATCTCAGCGA CAACAGAATC GAACAGATGG AAGAGGTCAG AAGCATCGGC
    AGCCTCCCGT GTCTGGAGCA CGTGGCTCTG CTGAACAACC CCCTGAGTAT CATTCCCGAC
    TACCGGACCA AGGTGCTGGC TCAGTTCGGA GAGCGGGCCT CGGAGGTCTG CCTGGACAAC
    ACAGTGACCA CAGAGAAAGA GTTGGACACA GTGGAAGTGC TAAAAGCGAT TCAGAAAGCC
    AAAGAGGTCA AGTCCAAATT AAGCAACCCT GAGAAGAAGG TCAGTGAGGA TTCTCGGCTC
    TCTGCTGCCC CCTGTGTCAG ACCCAGCAGC TCCCCACCCA GTGCGGCTCC CACCTCTGCC
    TCCCTGCCCC AGCCCATCCT CTCCAACCAA GGCATCCTCG GAGATGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu57731
    Clone ID Related Accession (Same CDS sequence) XM_025272431.1
    Accession Version XM_025272431.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product nischarin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGCGG CGGCGCGCAG CTTCGGGCCC GAGCGCGAGG CCGAGCCGGT CAAGACGGCG 
    CGCGTCGTGG GCTCTGAGCT CGTGGACACG TATACGGTTT ACATCATCCA GATCACTGAT
    GGGAGCCATG AGTGGACAGT CAAGCACCGC TACAGTGATT TCCACGACCT ACATGAAAAG
    CTTGTTGCGG AGAAGAAGAT TGATAGAAGT CTGCTTCCAC CCAAAAAGAT AATTGGGAAA
    AACTCCAGGA GCTTGGTGGA AAAGAGGGAG AAGGATCTCG AAATTTATCT CCAGACGCTC
    CTGGCCACCT TCCCTGACGT GGCCCCCAGA GTGCTGGCCC ACTTCTTGCA TTTCCACTTT
    TACGAGATTA ATGGCATCAC TGCAGCACTG GCTGAGGAAC TCTTCGAAAA AGGAGAACAG
    CTCCTGGGGG CTGGCGAGGT CTTCGCCATT GGACCCCTGC AGCTCTACGC AGTCACCGAG
    CAGCTGCAGC AGGGAAAGCC CACATGCGCC AGCGGGGATG CCAAGACTGA CCTGGGGCAC
    ATCCTGGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAAA AGGACCTTTT
    GGGACCAGCA ACATTCAGGA GCAGCTCCTG CCTTTTGATC TGTCCATATT CAAGTCTCTT
    CATCAGGTGG AGATAAGTCA CTGTGATGCC AGGCGCATCC GGGGGCTGGT TGCATCCAAG
    CCCACCTTGG CCACAATGAG CGTCCGTTTC TCGGCAACCT CCATGAAGGA AGTCCTCGTT
    CCTGAAGCCT CGGAGTTCGA TGAGTGGGAG CCAGCAGGCG CAGCCCTGGA GGGCCCCGTG
    ACTGCCGTCA TCCCCACATG GCAGGCTCTG ACCGCTCTGG ACTTGAGTCA CAATAGCATC
    TCTGAGATCG ATGATTCTGT GAAACTGATT CCAAAGATTG AGTTCCTGGA CCTGAGTCAC
    AATGGAGTGC TGGTCATGAA CAATTTGCAG CACCTGTACA ACCTTGTGCA CGTGGACCTG
    TCCTACAACA AGCTCTCCTC CTTGGAAGGG GTTCACACCA AACTGGGCAA CATCAAGACC
    CTGAACCTGG CAGGCAACCT CCTGAGGCAT CTGAGTGGCC TGCACAAGCT GTACTCCTTG
    GTCAACCTGG ATCTCAGCGA CAACAGAATC GAACAGATGG AAGAGGTCAG AAGCATCGGC
    AGCCTCCCGT GTCTGGAGCA CGTGGCTCTG CTGAACAACC CCCTGAGTAT CATTCCCGAC
    TACCGGACCA AGGTGCTGGC TCAGTTCGGA GAGCGGGCCT CGGAGGTCTG CCTGGACAAC
    ACAGTGACCA CAGAGAAAGA GTTGGACACA GTGGAAGTGC TAAAAGCGAT TCAGAAAGCC
    AAAGAGGTCA AGTCCAAATT AAGCAACCCT GAGAAGAAGG TCAGTGAGGA TTCTCGGCTC
    TCTGCTGCCC CCTGTGTCAG ACCCAGCAGC TCCCCACCCA GTGCGGCTCC CACCTCTGCC
    TCCCTGCCCC AGCCCATCCT CTCCAACCAA GGAATCATGT TTGTGCAAGA GGAGGCCCTG
    GCCAGCAGCC TCTCATCCAC CGACAGTCTG ACTCCTGAGG ACCGGTCCAT TGCGGGAGGA
    TGCTCTGATT CCTTGGAGTC CATCCCTGCG GGACAGGCAC CTTCTGATGA TTTAAGGGAT
    GTGCCAGGAG CTGTTGGCGG TGTGAGCCCC GAGCACGCGG AGCCAGAGGT CCAGGTGGTG
    CCTGGGTCCG GCCAGATAAT CTTCCTGCCC TTCACCTGCA TTGGCTACAC TGCCACCAAC
    CAGGACTTCA TCCAGCGCCT GAGCACGCTC ATCCGACAGG CTATTGAGCG GCAGCTCCCT
    GCTTGGATCG AGGCCGCCAA CCAGCGAGAG GAGGGCCAGG GGGAGCAGGG CGAGGAGGAG
    GAGGAGGAGG AGGACGTTGC TGAGAATCGC TACTTCGAAA TGGGGCCCCC AGATGCAGAG
    GAAGAGGAGG AGGGTGGGCC AGGCGAGGAA GAGGAAGAAG AGGAGGAGGA GGAGGAGGAG
    GCTGAGGAGG AGCGCCTGGC TCTGGAGTGG GCGCTGGGCG CAGACGAGGA CTTCCTGCTG
    GAGCATATCC GCATCCTCAA GGTGCTCTGG TGCTTCCTGA TCCACGTGCA GGGCAGCATC
    CGCCAGTTCG CCGCCTGCCT CGTGCTCACT GACTTCGGCA TCGCTGTCTT CGAGATCCCG
    CACCAGGAGT CGCGGGGCAG CAGCCAGCAC ATCCTTTCCT CACTGCGCTT CGTGTTCTGC
    TTCCCACACG GCGACCTCAC CGAGTTCGGC TTCCTCATGC CGGAGCTGTG CCTGGTGCTG
    AAGGTGCGGC ACAGCGAGAA CACACTCTTC ATCATCTCCG ACGCCGCCCA CCTGCACGAG
    TTCCACGCCG ACCTGCGCGC CTGCTTCGCC CCGCAGCACA TGGCCATGCT GTGCAGCCCC
    GTGCTCTACG GCAGCCACAC CAGCCTGCAG GAGTTCCTGC GCCAGCTGCT CACCTTCTAC
    AAGGTGGCAG GCGGCTGCCA GGAGCGCAGC CAGGGCTGCT TTCCCGTCTA CCTGGTCTAC
    AGTGACAAGC GCATGGTGCA GACGGCCGCC GGGGACTACT CGGGCAACAT CGAGTGGGCC
    AGCTGCACCC TCTGCTCGGC TGTGCGGCGC TCCTGCTGTG CGCCCTCCGA GGCCGTCAAG
    TCCGCCGCCA TCCCCTACTG GCTGTTGCTC ACGCCCCAGC ACCTCAACGT CATCAAGGCC
    GACTTCAACC CCATGCCCAA CCGGGGCACC CACAACTGCC GCAACCGCAA CAGCTTCAAG
    CTCAGCCGCG TGCCTCTCTC CACTGTGCTG CTGGACCCCA CGCGCAGCTG CACCCAGCCA
    CGGGGCGCCT TCGCAGATGG CCACGTGCTT GAGCTGCTCG TGGGCTACCG CTTCGTCACC
    GCCATCTTCG TGCTGCCCCA TGAGAAGTTC CACTTCCTGC GCATCTACAA CCAGCTGCGG
    GCCTCGCTGC AGGACCTGAA GACCGTGGTC ATTGCCAAGA CCCCTGCAAT GGGGGGCGGG
    CCTCAGAGCC CCCTTGTGGA CGGCCAGCCT GCAGAGAGCA GGGCCAGCAG TGACCAGAAT
    CTCCAAGAAG CTCCAGCAGA GACTGCAGCC CTGGCCCTGG TGGAGGCCCC TGCTCTCACT
    CCAGGAGAGG CCTCAGCCCC AAGCCCGGCA AAGACCCCAG CTCCAGTGGA GGCCTCAGTG
    CCAGCTCTTG TTCCAGTGGA GGTCCCCGCG GAGGCCGAGG CCTCAGCAGA GATCCCAGTG
    GAGGCTCTGG CCCAAGCAGA GGCCCCTGCA CAGTACCCAA GTGAGCCCCT GATCCGGTCC
    ATGTCGGAGG ACAATCAGAT CCCGTCACAC CTGCCTGCCT GCCCGTCTCT CCGGCACATC
    GCCAGCCTGC GTGGACGGGC CGTGGTCGAG TTCTTCCACA GCAGCATTGC CGAGGTGGAA
    AATGAGGAGC TGCGGCACCT CCTGTGGTCA TCGGTGGTGT TCTACCAGAC GCCGGGGCTG
    GAGGTGACAG CCTGCGTGCT GCTCTCTACC AAGGCCGTGT ACTTCGTGCT GCACGACGGC
    CTCCGCCGCT ACTTCTCGGA GCCGCTGCAG GATTTCTGGC ATCAGAAAAA CACCGACTAC
    AACAACAGCC CCTTCCACAT CTCTCAGTGC TTTGTTTTGA AACTCAGCGA CCTGCAGGTA
    GTCAACGTTG GGCTGTTCGA CCAGTATTTC CGGCTAACGG AAAAATGTCA CAGCCCTTCC
    GGCCTGAGAA ACGGCCGTCT CCCCAGGCCC CGTGATGCTT CGCCACGTGG CGCTTTCCTG
    GACCCGGCTC TGTGGTCCTC AGGTCCAGGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025128216.1
    CDS131..4003
    Translation

    Target ORF information:

    RefSeq Version XM_025272431.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis nischarin (NISCH), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025272431.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGCGG CGGCGCGCAG CTTCGGGCCC GAGCGCGAGG CCGAGCCGGT CAAGACGGCG 
    CGCGTCGTGG GCTCTGAGCT CGTGGACACG TATACGGTTT ACATCATCCA GATCACTGAT
    GGGAGCCATG AGTGGACAGT CAAGCACCGC TACAGTGATT TCCACGACCT ACATGAAAAG
    CTTGTTGCGG AGAAGAAGAT TGATAGAAGT CTGCTTCCAC CCAAAAAGAT AATTGGGAAA
    AACTCCAGGA GCTTGGTGGA AAAGAGGGAG AAGGATCTCG AAATTTATCT CCAGACGCTC
    CTGGCCACCT TCCCTGACGT GGCCCCCAGA GTGCTGGCCC ACTTCTTGCA TTTCCACTTT
    TACGAGATTA ATGGCATCAC TGCAGCACTG GCTGAGGAAC TCTTCGAAAA AGGAGAACAG
    CTCCTGGGGG CTGGCGAGGT CTTCGCCATT GGACCCCTGC AGCTCTACGC AGTCACCGAG
    CAGCTGCAGC AGGGAAAGCC CACATGCGCC AGCGGGGATG CCAAGACTGA CCTGGGGCAC
    ATCCTGGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAAA AGGACCTTTT
    GGGACCAGCA ACATTCAGGA GCAGCTCCTG CCTTTTGATC TGTCCATATT CAAGTCTCTT
    CATCAGGTGG AGATAAGTCA CTGTGATGCC AGGCGCATCC GGGGGCTGGT TGCATCCAAG
    CCCACCTTGG CCACAATGAG CGTCCGTTTC TCGGCAACCT CCATGAAGGA AGTCCTCGTT
    CCTGAAGCCT CGGAGTTCGA TGAGTGGGAG CCAGCAGGCG CAGCCCTGGA GGGCCCCGTG
    ACTGCCGTCA TCCCCACATG GCAGGCTCTG ACCGCTCTGG ACTTGAGTCA CAATAGCATC
    TCTGAGATCG ATGATTCTGT GAAACTGATT CCAAAGATTG AGTTCCTGGA CCTGAGTCAC
    AATGGAGTGC TGGTCATGAA CAATTTGCAG CACCTGTACA ACCTTGTGCA CGTGGACCTG
    TCCTACAACA AGCTCTCCTC CTTGGAAGGG GTTCACACCA AACTGGGCAA CATCAAGACC
    CTGAACCTGG CAGGCAACCT CCTGAGGCAT CTGAGTGGCC TGCACAAGCT GTACTCCTTG
    GTCAACCTGG ATCTCAGCGA CAACAGAATC GAACAGATGG AAGAGGTCAG AAGCATCGGC
    AGCCTCCCGT GTCTGGAGCA CGTGGCTCTG CTGAACAACC CCCTGAGTAT CATTCCCGAC
    TACCGGACCA AGGTGCTGGC TCAGTTCGGA GAGCGGGCCT CGGAGGTCTG CCTGGACAAC
    ACAGTGACCA CAGAGAAAGA GTTGGACACA GTGGAAGTGC TAAAAGCGAT TCAGAAAGCC
    AAAGAGGTCA AGTCCAAATT AAGCAACCCT GAGAAGAAGG TCAGTGAGGA TTCTCGGCTC
    TCTGCTGCCC CCTGTGTCAG ACCCAGCAGC TCCCCACCCA GTGCGGCTCC CACCTCTGCC
    TCCCTGCCCC AGCCCATCCT CTCCAACCAA GGAATCATGT TTGTGCAAGA GGAGGCCCTG
    GCCAGCAGCC TCTCATCCAC CGACAGTCTG ACTCCTGAGG ACCGGTCCAT TGCGGGAGGA
    TGCTCTGATT CCTTGGAGTC CATCCCTGCG GGACAGGCAC CTTCTGATGA TTTAAGGGAT
    GTGCCAGGAG CTGTTGGCGG TGTGAGCCCC GAGCACGCGG AGCCAGAGGT CCAGGTGGTG
    CCTGGGTCCG GCCAGATAAT CTTCCTGCCC TTCACCTGCA TTGGCTACAC TGCCACCAAC
    CAGGACTTCA TCCAGCGCCT GAGCACGCTC ATCCGACAGG CTATTGAGCG GCAGCTCCCT
    GCTTGGATCG AGGCCGCCAA CCAGCGAGAG GAGGGCCAGG GGGAGCAGGG CGAGGAGGAG
    GAGGAGGAGG AGGACGTTGC TGAGAATCGC TACTTCGAAA TGGGGCCCCC AGATGCAGAG
    GAAGAGGAGG AGGGTGGGCC AGGCGAGGAA GAGGAAGAAG AGGAGGAGGA GGAGGAGGAG
    GCTGAGGAGG AGCGCCTGGC TCTGGAGTGG GCGCTGGGCG CAGACGAGGA CTTCCTGCTG
    GAGCATATCC GCATCCTCAA GGTGCTCTGG TGCTTCCTGA TCCACGTGCA GGGCAGCATC
    CGCCAGTTCG CCGCCTGCCT CGTGCTCACT GACTTCGGCA TCGCTGTCTT CGAGATCCCG
    CACCAGGAGT CGCGGGGCAG CAGCCAGCAC ATCCTTTCCT CACTGCGCTT CGTGTTCTGC
    TTCCCACACG GCGACCTCAC CGAGTTCGGC TTCCTCATGC CGGAGCTGTG CCTGGTGCTG
    AAGGTGCGGC ACAGCGAGAA CACACTCTTC ATCATCTCCG ACGCCGCCCA CCTGCACGAG
    TTCCACGCCG ACCTGCGCGC CTGCTTCGCC CCGCAGCACA TGGCCATGCT GTGCAGCCCC
    GTGCTCTACG GCAGCCACAC CAGCCTGCAG GAGTTCCTGC GCCAGCTGCT CACCTTCTAC
    AAGGTGGCAG GCGGCTGCCA GGAGCGCAGC CAGGGCTGCT TTCCCGTCTA CCTGGTCTAC
    AGTGACAAGC GCATGGTGCA GACGGCCGCC GGGGACTACT CGGGCAACAT CGAGTGGGCC
    AGCTGCACCC TCTGCTCGGC TGTGCGGCGC TCCTGCTGTG CGCCCTCCGA GGCCGTCAAG
    TCCGCCGCCA TCCCCTACTG GCTGTTGCTC ACGCCCCAGC ACCTCAACGT CATCAAGGCC
    GACTTCAACC CCATGCCCAA CCGGGGCACC CACAACTGCC GCAACCGCAA CAGCTTCAAG
    CTCAGCCGCG TGCCTCTCTC CACTGTGCTG CTGGACCCCA CGCGCAGCTG CACCCAGCCA
    CGGGGCGCCT TCGCAGATGG CCACGTGCTT GAGCTGCTCG TGGGCTACCG CTTCGTCACC
    GCCATCTTCG TGCTGCCCCA TGAGAAGTTC CACTTCCTGC GCATCTACAA CCAGCTGCGG
    GCCTCGCTGC AGGACCTGAA GACCGTGGTC ATTGCCAAGA CCCCTGCAAT GGGGGGCGGG
    CCTCAGAGCC CCCTTGTGGA CGGCCAGCCT GCAGAGAGCA GGGCCAGCAG TGACCAGAAT
    CTCCAAGAAG CTCCAGCAGA GACTGCAGCC CTGGCCCTGG TGGAGGCCCC TGCTCTCACT
    CCAGGAGAGG CCTCAGCCCC AAGCCCGGCA AAGACCCCAG CTCCAGTGGA GGCCTCAGTG
    CCAGCTCTTG TTCCAGTGGA GGTCCCCGCG GAGGCCGAGG CCTCAGCAGA GATCCCAGTG
    GAGGCTCTGG CCCAAGCAGA GGCCCCTGCA CAGTACCCAA GTGAGCCCCT GATCCGGTCC
    ATGTCGGAGG ACAATCAGAT CCCGTCACAC CTGCCTGCCT GCCCGTCTCT CCGGCACATC
    GCCAGCCTGC GTGGACGGGC CGTGGTCGAG TTCTTCCACA GCAGCATTGC CGAGGTGGAA
    AATGAGGAGC TGCGGCACCT CCTGTGGTCA TCGGTGGTGT TCTACCAGAC GCCGGGGCTG
    GAGGTGACAG CCTGCGTGCT GCTCTCTACC AAGGCCGTGT ACTTCGTGCT GCACGACGGC
    CTCCGCCGCT ACTTCTCGGA GCCGCTGCAG GATTTCTGGC ATCAGAAAAA CACCGACTAC
    AACAACAGCC CCTTCCACAT CTCTCAGTGC TTTGTTTTGA AACTCAGCGA CCTGCAGGTA
    GTCAACGTTG GGCTGTTCGA CCAGTATTTC CGGCTAACGG AAAAATGTCA CAGCCCTTCC
    GGCCTGAGAA ACGGCCGTCT CCCCAGGCCC CGTGATGCTT CGCCACGTGG CGCTTTCCTG
    GACCCGGCTC TGTGGTCCTC AGGTCCAGGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu57730
    Clone ID Related Accession (Same CDS sequence) XM_006044040.2
    Accession Version XM_006044040.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product nischarin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006044040.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGGCGG CGGCGCGCAG CTTCGGGCCC GAGCGCGAGG CCGAGCCGGT CAAGACGGCG 
    CGCGTCGTGG GCTCTGAGCT CGTGGACACG TATACGGTTT ACATCATCCA GATCACTGAT
    GGGAGCCATG AGTGGACAGT CAAGCACCGC TACAGTGATT TCCACGACCT ACATGAAAAG
    CTTGTTGCGG AGAAGAAGAT TGATAGAAGT CTGCTTCCAC CCAAAAAGAT AATTGGGAAA
    AACTCCAGGA GCTTGGTGGA AAAGAGGGAG AAGGATCTCG AAATTTATCT CCAGACGCTC
    CTGGCCACCT TCCCTGACGT GGCCCCCAGA GTGCTGGCCC ACTTCTTGCA TTTCCACTTT
    TACGAGATTA ATGGCATCAC TGCAGCACTG GCTGAGGAAC TCTTCGAAAA AGGAGAACAG
    CTCCTGGGGG CTGGCGAGGT CTTCGCCATT GGACCCCTGC AGCTCTACGC AGTCACCGAG
    CAGCTGCAGC AGGGAAAGCC CACATGCGCC AGCGGGGATG CCAAGACTGA CCTGGGGCAC
    ATCCTGGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAAA AGGACCTTTT
    GGGACCAGCA ACATTCAGGA GCAGCTCCTG CCTTTTGATC TGTCCATATT CAAGTCTCTT
    CATCAGGTGG AGATAAGTCA CTGTGATGCC AGGCGCATCC GGGGGCTGGT TGCATCCAAG
    CCCACCTTGG CCACAATGAG CGTCCGTTTC TCGGCAACCT CCATGAAGGA AGTCCTCGTT
    CCTGAAGCCT CGGAGTTCGA TGAGTGGGAG CCAGCAGGCG CAGCCCTGGA GGGCCCCGTG
    ACTGCCGTCA TCCCCACATG GCAGGCTCTG ACCGCTCTGG ACTTGAGTCA CAATAGCATC
    TCTGAGATCG ATGATTCTGT GAAACTGATT CCAAAGATTG AGTTCCTGGA CCTGAGTCAC
    AATGGAGTGC TGGTCATGAA CAATTTGCAG CACCTGTACA ACCTTGTGCA CGTGGACCTG
    TCCTACAACA AGCTCTCCTC CTTGGAAGGG GTTCACACCA AACTGGGCAA CATCAAGACC
    CTGAACCTGG CAGGCAACCT CCTGAGGCAT CTGAGTGGCC TGCACAAGCT GTACTCCTTG
    GTCAACCTGG ATCTCAGCGA CAACAGAATC GAACAGATGG AAGAGGTCAG AAGCATCGGC
    AGCCTCCCGT GTCTGGAGCA CGTGGCTCTG CTGAACAACC CCCTGAGTAT CATTCCCGAC
    TACCGGACCA AGGTGCTGGC TCAGTTCGGA GAGCGGGCCT CGGAGGTCTG CCTGGACAAC
    ACAGTGACCA CAGAGAAAGA GTTGGACACA GTGGAAGTGC TAAAAGCGAT TCAGAAAGCC
    AAAGAGGTCA AGTCCAAATT AAGCAACCCT GAGAAGAAGG TCAGTGAGGA TTCTCGGCTC
    TCTGCTGCCC CCTGTGTCAG ACCCAGCAGC TCCCCACCCA GTGCGGCTCC CACCTCTGCC
    TCCCTGCCCC AGCCCATCCT CTCCAACCAA GGAATCATGT TTGTGCAAGA GGAGGCCCTG
    GCCAGCAGCC TCTCATCCAC CGACAGTCTG ACTCCTGAGG ACCGGTCCAT TGCGGGAGGA
    TGCTCTGATT CCTTGGAGTC CATCCCTGCG GGACAGGCAC CTTCTGATGA TTTAAGGGAT
    GTGCCAGGAG CTGTTGGCGG TGTGAGCCCC GAGCACGCGG AGCCAGAGGT CCAGGTGGTG
    CCTGGGTCCG GCCAGATAAT CTTCCTGCCC TTCACCTGCA TTGGCTACAC TGCCACCAAC
    CAGGACTTCA TCCAGCGCCT GAGCACGCTC ATCCGACAGG CTATTGAGCG GCAGCTCCCT
    GCTTGGATCG AGGCCGCCAA CCAGCGAGAG GAGGGCCAGG GGGAGCAGGG CGAGGAGGAG
    GAGGAGGAGG AGGACGTTGC TGAGAATCGC TACTTCGAAA TGGGGCCCCC AGATGCAGAG
    GAAGAGGAGG AGGGTGGGCC AGGCGAGGAA GAGGAAGAAG AGGAGGAGGA GGAGGAGGAG
    GCTGAGGAGG AGCGCCTGGC TCTGGAGTGG GCGCTGGGCG CAGACGAGGA CTTCCTGCTG
    GAGCATATCC GCATCCTCAA GGTGCTCTGG TGCTTCCTGA TCCACGTGCA GGGCAGCATC
    CGCCAGTTCG CCGCCTGCCT CGTGCTCACT GACTTCGGCA TCGCTGTCTT CGAGATCCCG
    CACCAGGAGT CGCGGGGCAG CAGCCAGCAC ATCCTTTCCT CACTGCGCTT CGTGTTCTGC
    TTCCCACACG GCGACCTCAC CGAGTTCGGC TTCCTCATGC CGGAGCTGTG CCTGGTGCTG
    AAGGTGCGGC ACAGCGAGAA CACACTCTTC ATCATCTCCG ACGCCGCCCA CCTGCACGAG
    TTCCACGCCG ACCTGCGCGC CTGCTTCGCC CCGCAGCACA TGGCCATGCT GTGCAGCCCC
    GTGCTCTACG GCAGCCACAC CAGCCTGCAG GAGTTCCTGC GCCAGCTGCT CACCTTCTAC
    AAGGTGGCAG GCGGCTGCCA GGAGCGCAGC CAGGGCTGCT TTCCCGTCTA CCTGGTCTAC
    AGTGACAAGC GCATGGTGCA GACGGCCGCC GGGGACTACT CGGGCAACAT CGAGTGGGCC
    AGCTGCACCC TCTGCTCGGC TGTGCGGCGC TCCTGCTGTG CGCCCTCCGA GGCCGTCAAG
    TCCGCCGCCA TCCCCTACTG GCTGTTGCTC ACGCCCCAGC ACCTCAACGT CATCAAGGCC
    GACTTCAACC CCATGCCCAA CCGGGGCACC CACAACTGCC GCAACCGCAA CAGCTTCAAG
    CTCAGCCGCG TGCCTCTCTC CACTGTGCTG CTGGACCCCA CGCGCAGCTG CACCCAGCCA
    CGGGGCGCCT TCGCAGATGG CCACGTGCTT GAGCTGCTCG TGGGCTACCG CTTCGTCACC
    GCCATCTTCG TGCTGCCCCA TGAGAAGTTC CACTTCCTGC GCATCTACAA CCAGCTGCGG
    GCCTCGCTGC AGGACCTGAA GACCGTGGTC ATTGCCAAGA CCCCTGCAAT GGGGGGCGGG
    CCTCAGAGCC CCCTTGTGGA CGGCCAGCCT GCAGAGAGCA GGGCCAGCAG TGACCAGAAT
    CTCCAAGAAG CTCCAGCAGA GACTGCAGCC CTGGCCCTGG TGGAGGCCCC TGCTCTCACT
    CCAGGAGAGG CCTCAGCCCC AAGCCCGGCA AAGACCCCAG CTCCAGTGGA GGCCTCAGTG
    CCAGCTCTTG TTCCAGTGGA GGTCCCCGCG GAGGCCGAGG CCTCAGCAGA GATCCCAGTG
    GAGGCTCTGG CCCAAGCAGA GGCCCCTGCA CAGTACCCAA GTGAGCCCCT GATCCGGTCC
    ATGTCGGAGG ACAATCAGAT CCCGTCACAC CTGCCTGCCT GCCCGTCTCT CCGGCACATC
    GCCAGCCTGC GTGGACGGGC CGTGGTCGAG TTCTTCCACA GCAGCATTGC CGAGGTGGAA
    AATGAGGAGC TGCGGCACCT CCTGTGGTCA TCGGTGGTGT TCTACCAGAC GCCGGGGCTG
    GAGGTGACAG CCTGCGTGCT GCTCTCTACC AAGGCCGTGT ACTTCGTGCT GCACGACGGC
    CTCCGCCGCT ACTTCTCGGA GCCGCTGCAG GATTTCTGGC ATCAGAAAAA CACCGACTAC
    AACAACAGCC CCTTCCACAT CTCTCAGTGC TTTGTTTTGA AACTCAGCGA CCTGCAGGTA
    GTCAACGTTG GGCTGTTCGA CCAGTATTTC CGGCTAACGG GCTCCTGCCC GGCACAGGTG
    GTTACCTGCT TGACGCGGGA TAGCTACCTG ACACACTGCT TCCTCCAGCA CCTCATGGCT
    GTGCTCTCCC TGCTGGAACG CACACCCTCG CCTGAGCCTG TGGACAAAGA CTTCTATTCT
    GAGTTTGGAA ACAAGACCAC AGGGAAGATG GAAAACTATG AGCTGATCCA TTCGAGCCGC
    GTCAAGTTCA CCTACCCCAG CGAGGAGGAG GTCGGAGACC TGACGTTCAC TGTGGCCCAG
    AAGATGGCTG TGCCGGAGAA GGCGCCGGCC CTCAGCATCC TGCTGTGCGT GCAGGCCTTC
    CAGGTGGGCT CGCCGCAGCC GGGGCGCTGC AGGGGCTTGC TGCGCCCCAA GACGCTCCTG
    CTCACCAGCG CCGAGATCTT CCTCCTGGAC GAGGACTTCA TCCACTACCC GCTGCCCGAG
    TTCGCCAAAG AGCCGCCACA GAGGGACCGG TACCGGCTGG ACGACGGGCG CCGCGTCCGG
    GACCTGGACC GCGTGCTCAT GGGCTACCAG ACATACCCAC AGGCCCTCAC CCTGGTCTTC
    GATGATGTGC AGGGCCACGA CCTCATGGGC AGCGTCACCC TGGACCACTT CGGGGAGGTG
    CCAGGTGGCC CGGCCAGGGG TGGCCAGGGC CGTGAGGTCC AGTGGCAGGT GTTTGTCCCC
    AGCGCTGAGA GCCGAGAGAA GCTCATCTCG CTGCTGGCCC GTCAGTGGGA GGCCCTGTGT
    GGCCGGGAGC TGCCCGTGGA GCTCACCGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006044102.2
    CDS405..4937
    Translation

    Target ORF information:

    RefSeq Version XM_006044040.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis nischarin (NISCH), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006044040.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGGCGG CGGCGCGCAG CTTCGGGCCC GAGCGCGAGG CCGAGCCGGT CAAGACGGCG 
    CGCGTCGTGG GCTCTGAGCT CGTGGACACG TATACGGTTT ACATCATCCA GATCACTGAT
    GGGAGCCATG AGTGGACAGT CAAGCACCGC TACAGTGATT TCCACGACCT ACATGAAAAG
    CTTGTTGCGG AGAAGAAGAT TGATAGAAGT CTGCTTCCAC CCAAAAAGAT AATTGGGAAA
    AACTCCAGGA GCTTGGTGGA AAAGAGGGAG AAGGATCTCG AAATTTATCT CCAGACGCTC
    CTGGCCACCT TCCCTGACGT GGCCCCCAGA GTGCTGGCCC ACTTCTTGCA TTTCCACTTT
    TACGAGATTA ATGGCATCAC TGCAGCACTG GCTGAGGAAC TCTTCGAAAA AGGAGAACAG
    CTCCTGGGGG CTGGCGAGGT CTTCGCCATT GGACCCCTGC AGCTCTACGC AGTCACCGAG
    CAGCTGCAGC AGGGAAAGCC CACATGCGCC AGCGGGGATG CCAAGACTGA CCTGGGGCAC
    ATCCTGGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAAA AGGACCTTTT
    GGGACCAGCA ACATTCAGGA GCAGCTCCTG CCTTTTGATC TGTCCATATT CAAGTCTCTT
    CATCAGGTGG AGATAAGTCA CTGTGATGCC AGGCGCATCC GGGGGCTGGT TGCATCCAAG
    CCCACCTTGG CCACAATGAG CGTCCGTTTC TCGGCAACCT CCATGAAGGA AGTCCTCGTT
    CCTGAAGCCT CGGAGTTCGA TGAGTGGGAG CCAGCAGGCG CAGCCCTGGA GGGCCCCGTG
    ACTGCCGTCA TCCCCACATG GCAGGCTCTG ACCGCTCTGG ACTTGAGTCA CAATAGCATC
    TCTGAGATCG ATGATTCTGT GAAACTGATT CCAAAGATTG AGTTCCTGGA CCTGAGTCAC
    AATGGAGTGC TGGTCATGAA CAATTTGCAG CACCTGTACA ACCTTGTGCA CGTGGACCTG
    TCCTACAACA AGCTCTCCTC CTTGGAAGGG GTTCACACCA AACTGGGCAA CATCAAGACC
    CTGAACCTGG CAGGCAACCT CCTGAGGCAT CTGAGTGGCC TGCACAAGCT GTACTCCTTG
    GTCAACCTGG ATCTCAGCGA CAACAGAATC GAACAGATGG AAGAGGTCAG AAGCATCGGC
    AGCCTCCCGT GTCTGGAGCA CGTGGCTCTG CTGAACAACC CCCTGAGTAT CATTCCCGAC
    TACCGGACCA AGGTGCTGGC TCAGTTCGGA GAGCGGGCCT CGGAGGTCTG CCTGGACAAC
    ACAGTGACCA CAGAGAAAGA GTTGGACACA GTGGAAGTGC TAAAAGCGAT TCAGAAAGCC
    AAAGAGGTCA AGTCCAAATT AAGCAACCCT GAGAAGAAGG TCAGTGAGGA TTCTCGGCTC
    TCTGCTGCCC CCTGTGTCAG ACCCAGCAGC TCCCCACCCA GTGCGGCTCC CACCTCTGCC
    TCCCTGCCCC AGCCCATCCT CTCCAACCAA GGAATCATGT TTGTGCAAGA GGAGGCCCTG
    GCCAGCAGCC TCTCATCCAC CGACAGTCTG ACTCCTGAGG ACCGGTCCAT TGCGGGAGGA
    TGCTCTGATT CCTTGGAGTC CATCCCTGCG GGACAGGCAC CTTCTGATGA TTTAAGGGAT
    GTGCCAGGAG CTGTTGGCGG TGTGAGCCCC GAGCACGCGG AGCCAGAGGT CCAGGTGGTG
    CCTGGGTCCG GCCAGATAAT CTTCCTGCCC TTCACCTGCA TTGGCTACAC TGCCACCAAC
    CAGGACTTCA TCCAGCGCCT GAGCACGCTC ATCCGACAGG CTATTGAGCG GCAGCTCCCT
    GCTTGGATCG AGGCCGCCAA CCAGCGAGAG GAGGGCCAGG GGGAGCAGGG CGAGGAGGAG
    GAGGAGGAGG AGGACGTTGC TGAGAATCGC TACTTCGAAA TGGGGCCCCC AGATGCAGAG
    GAAGAGGAGG AGGGTGGGCC AGGCGAGGAA GAGGAAGAAG AGGAGGAGGA GGAGGAGGAG
    GCTGAGGAGG AGCGCCTGGC TCTGGAGTGG GCGCTGGGCG CAGACGAGGA CTTCCTGCTG
    GAGCATATCC GCATCCTCAA GGTGCTCTGG TGCTTCCTGA TCCACGTGCA GGGCAGCATC
    CGCCAGTTCG CCGCCTGCCT CGTGCTCACT GACTTCGGCA TCGCTGTCTT CGAGATCCCG
    CACCAGGAGT CGCGGGGCAG CAGCCAGCAC ATCCTTTCCT CACTGCGCTT CGTGTTCTGC
    TTCCCACACG GCGACCTCAC CGAGTTCGGC TTCCTCATGC CGGAGCTGTG CCTGGTGCTG
    AAGGTGCGGC ACAGCGAGAA CACACTCTTC ATCATCTCCG ACGCCGCCCA CCTGCACGAG
    TTCCACGCCG ACCTGCGCGC CTGCTTCGCC CCGCAGCACA TGGCCATGCT GTGCAGCCCC
    GTGCTCTACG GCAGCCACAC CAGCCTGCAG GAGTTCCTGC GCCAGCTGCT CACCTTCTAC
    AAGGTGGCAG GCGGCTGCCA GGAGCGCAGC CAGGGCTGCT TTCCCGTCTA CCTGGTCTAC
    AGTGACAAGC GCATGGTGCA GACGGCCGCC GGGGACTACT CGGGCAACAT CGAGTGGGCC
    AGCTGCACCC TCTGCTCGGC TGTGCGGCGC TCCTGCTGTG CGCCCTCCGA GGCCGTCAAG
    TCCGCCGCCA TCCCCTACTG GCTGTTGCTC ACGCCCCAGC ACCTCAACGT CATCAAGGCC
    GACTTCAACC CCATGCCCAA CCGGGGCACC CACAACTGCC GCAACCGCAA CAGCTTCAAG
    CTCAGCCGCG TGCCTCTCTC CACTGTGCTG CTGGACCCCA CGCGCAGCTG CACCCAGCCA
    CGGGGCGCCT TCGCAGATGG CCACGTGCTT GAGCTGCTCG TGGGCTACCG CTTCGTCACC
    GCCATCTTCG TGCTGCCCCA TGAGAAGTTC CACTTCCTGC GCATCTACAA CCAGCTGCGG
    GCCTCGCTGC AGGACCTGAA GACCGTGGTC ATTGCCAAGA CCCCTGCAAT GGGGGGCGGG
    CCTCAGAGCC CCCTTGTGGA CGGCCAGCCT GCAGAGAGCA GGGCCAGCAG TGACCAGAAT
    CTCCAAGAAG CTCCAGCAGA GACTGCAGCC CTGGCCCTGG TGGAGGCCCC TGCTCTCACT
    CCAGGAGAGG CCTCAGCCCC AAGCCCGGCA AAGACCCCAG CTCCAGTGGA GGCCTCAGTG
    CCAGCTCTTG TTCCAGTGGA GGTCCCCGCG GAGGCCGAGG CCTCAGCAGA GATCCCAGTG
    GAGGCTCTGG CCCAAGCAGA GGCCCCTGCA CAGTACCCAA GTGAGCCCCT GATCCGGTCC
    ATGTCGGAGG ACAATCAGAT CCCGTCACAC CTGCCTGCCT GCCCGTCTCT CCGGCACATC
    GCCAGCCTGC GTGGACGGGC CGTGGTCGAG TTCTTCCACA GCAGCATTGC CGAGGTGGAA
    AATGAGGAGC TGCGGCACCT CCTGTGGTCA TCGGTGGTGT TCTACCAGAC GCCGGGGCTG
    GAGGTGACAG CCTGCGTGCT GCTCTCTACC AAGGCCGTGT ACTTCGTGCT GCACGACGGC
    CTCCGCCGCT ACTTCTCGGA GCCGCTGCAG GATTTCTGGC ATCAGAAAAA CACCGACTAC
    AACAACAGCC CCTTCCACAT CTCTCAGTGC TTTGTTTTGA AACTCAGCGA CCTGCAGGTA
    GTCAACGTTG GGCTGTTCGA CCAGTATTTC CGGCTAACGG GCTCCTGCCC GGCACAGGTG
    GTTACCTGCT TGACGCGGGA TAGCTACCTG ACACACTGCT TCCTCCAGCA CCTCATGGCT
    GTGCTCTCCC TGCTGGAACG CACACCCTCG CCTGAGCCTG TGGACAAAGA CTTCTATTCT
    GAGTTTGGAA ACAAGACCAC AGGGAAGATG GAAAACTATG AGCTGATCCA TTCGAGCCGC
    GTCAAGTTCA CCTACCCCAG CGAGGAGGAG GTCGGAGACC TGACGTTCAC TGTGGCCCAG
    AAGATGGCTG TGCCGGAGAA GGCGCCGGCC CTCAGCATCC TGCTGTGCGT GCAGGCCTTC
    CAGGTGGGCT CGCCGCAGCC GGGGCGCTGC AGGGGCTTGC TGCGCCCCAA GACGCTCCTG
    CTCACCAGCG CCGAGATCTT CCTCCTGGAC GAGGACTTCA TCCACTACCC GCTGCCCGAG
    TTCGCCAAAG AGCCGCCACA GAGGGACCGG TACCGGCTGG ACGACGGGCG CCGCGTCCGG
    GACCTGGACC GCGTGCTCAT GGGCTACCAG ACATACCCAC AGGCCCTCAC CCTGGTCTTC
    GATGATGTGC AGGGCCACGA CCTCATGGGC AGCGTCACCC TGGACCACTT CGGGGAGGTG
    CCAGGTGGCC CGGCCAGGGG TGGCCAGGGC CGTGAGGTCC AGTGGCAGGT GTTTGTCCCC
    AGCGCTGAGA GCCGAGAGAA GCTCATCTCG CTGCTGGCCC GTCAGTGGGA GGCCCTGTGT
    GGCCGGGAGC TGCCCGTGGA GCTCACCGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu03272
    Clone ID Related Accession (Same CDS sequence) XM_006044040.1
    Accession Version XM_006044040.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product nischarin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783532.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGGCGG CGGCGCGCAG CTTCGGGCCC GAGCGCGAGG CCGAGCCGGT CAAGACGGCG 
    CGCGTCGTGG GCTCTGAGCT CGTGGACACG TATACGGTTT ACATCATCCA GATCACTGAT
    GGGAGCCATG AGTGGACAGT CAAGCACCGC TACAGTGATT TCCACGACCT ACATGAAAAG
    CTTGTTGCGG AGAAGAAGAT TGATAGAAGT CTGCTTCCAC CCAAAAAGAT AATTGGGAAA
    AACTCCAGGA GCTTGGTGGA AAAGAGGGAG AAGGATCTCG AAATTTATCT CCAGACGCTC
    CTGGCCACCT TCCCTGACGT GGCCCCCAGA GTGCTGGCCC ACTTCTTGCA TTTCCACTTT
    TACGAGATTA ATGGCATCAC TGCAGCACTG GCTGAGGAAC TCTTCGAAAA AGGAGAACAG
    CTCCTGGGGG CTGGCGAGGT CTTCGCCATT GGACCCCTGC AGCTCTACGC AGTCACCGAG
    CAGCTGCAGC AGGGAAAGCC CACATGCGCC AGCGGGGATG CCAAGACTGA CCTGGGGCAC
    ATCCTGGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAAA AGGACCTTTT
    GGGACCAGCA ACATTCAGGA GCAGCTCCTG CCTTTTGATC TGTCCATATT CAAGTCTCTT
    CATCAGGTGG AGATAAGTCA CTGTGATGCC AGGCGCATCC GGGGGCTGGT TGCATCCAAG
    CCCACCTTGG CCACAATGAG CGTCCGTTTC TCGGCAACCT CCATGAAGGA AGTCCTCGTT
    CCTGAAGCCT CGGAGTTCGA TGAGTGGGAG CCAGCAGGCG CAGCCCTGGA GGGCCCCGTG
    ACTGCCGTCA TCCCCACATG GCAGGCTCTG ACCGCTCTGG ACTTGAGTCA CAATAGCATC
    TCTGAGATCG ATGATTCTGT GAAACTGATT CCAAAGATTG AGTTCCTGGA CCTGAGTCAC
    AATGGAGTGC TGGTCATGAA CAATTTGCAG CACCTGTACA ACCTTGTGCA CGTGGACCTG
    TCCTACAACA AGCTCTCCTC CTTGGAAGGG GTTCACACCA AACTGGGCAA CATCAAGACC
    CTGAACCTGG CAGGCAACCT CCTGAGGCAT CTGAGTGGCC TGCACAAGCT GTACTCCTTG
    GTCAACCTGG ATCTCAGCGA CAACAGAATC GAACAGATGG AAGAGGTCAG AAGCATCGGC
    AGCCTCCCGT GTCTGGAGCA CGTGGCTCTG CTGAACAACC CCCTGAGTAT CATTCCCGAC
    TACCGGACCA AGGTGCTGGC TCAGTTCGGA GAGCGGGCCT CGGAGGTCTG CCTGGACAAC
    ACAGTGACCA CAGAGAAAGA GTTGGACACA GTGGAAGTGC TAAAAGCGAT TCAGAAAGCC
    AAAGAGGTCA AGTCCAAATT AAGCAACCCT GAGAAGAAGG TCAGTGAGGA TTCTCGGCTC
    TCTGCTGCCC CCTGTGTCAG ACCCAGCAGC TCCCCACCCA GTGCGGCTCC CACCTCTGCC
    TCCCTGCCCC AGCCCATCCT CTCCAACCAA GGAATCATGT TTGTGCAAGA GGAGGCCCTG
    GCCAGCAGCC TCTCATCCAC CGACAGTCTG ACTCCTGAGG ACCGGTCCAT TGCGGGAGGA
    TGCTCTGATT CCTTGGAGTC CATCCCTGCG GGACAGGCAC CTTCTGATGA TTTAAGGGAT
    GTGCCAGGAG CTGTTGGCGG TGTGAGCCCC GAGCACGCGG AGCCAGAGGT CCAGGTGGTG
    CCTGGGTCCG GCCAGATAAT CTTCCTGCCC TTCACCTGCA TTGGCTACAC TGCCACCAAC
    CAGGACTTCA TCCAGCGCCT GAGCACGCTC ATCCGACAGG CTATTGAGCG GCAGCTCCCT
    GCTTGGATCG AGGCCGCCAA CCAGCGAGAG GAGGGCCAGG GGGAGCAGGG CGAGGAGGAG
    GAGGAGGAGG AGGACGTTGC TGAGAATCGC TACTTCGAAA TGGGGCCCCC AGATGCAGAG
    GAAGAGGAGG AGGGTGGGCC AGGCGAGGAA GAGGAAGAAG AGGAGGAGGA GGAGGAGGAG
    GCTGAGGAGG AGCGCCTGGC TCTGGAGTGG GCGCTGGGCG CAGACGAGGA CTTCCTGCTG
    GAGCATATCC GCATCCTCAA GGTGCTCTGG TGCTTCCTGA TCCACGTGCA GGGCAGCATC
    CGCCAGTTCG CCGCCTGCCT CGTGCTCACT GACTTCGGCA TCGCTGTCTT CGAGATCCCG
    CACCAGGAGT CGCGGGGCAG CAGCCAGCAC ATCCTTTCCT CACTGCGCTT CGTGTTCTGC
    TTCCCACACG GCGACCTCAC CGAGTTCGGC TTCCTCATGC CGGAGCTGTG CCTGGTGCTG
    AAGGTGCGGC ACAGCGAGAA CACACTCTTC ATCATCTCCG ACGCCGCCCA CCTGCACGAG
    TTCCACGCCG ACCTGCGCGC CTGCTTCGCC CCGCAGCACA TGGCCATGCT GTGCAGCCCC
    GTGCTCTACG GCAGCCACAC CAGCCTGCAG GAGTTCCTGC GCCAGCTGCT CACCTTCTAC
    AAGGTGGCAG GCGGCTGCCA GGAGCGCAGC CAGGGCTGCT TTCCCGTCTA CCTGGTCTAC
    AGTGACAAGC GCATGGTGCA GACAGCCGCC GGGGACTACT CGGGCAACAT CGAGTGGGCC
    AGCTGCACCC TCTGCTCGGC TGTGCGGCGC TCCTGCTGTG CGCCCTCCGA GGCCGTCAAG
    TCCGCCGCCA TCCCCTACTG GCTGTTGCTC ACGCCCCAGC ACCTCAACGT CATCAAGGCC
    GACTTCAACC CCATGCCCAA CCGGGGCACC CACAACTGCC GCAACCGCAA CAGCTTCAAG
    CTCAGCCGCG TGCCTCTCTC CACTGTGCTG CTGGACCCCA CGCGCAGCTG CACCCAGCCA
    CGGGGCGCCT TCGCAGATGG CCACGTGCTT GAGCTGCTCG TGGGCTACCG CTTCGTCACC
    GCCATCTTCG TGCTGCCCCA TGAGAAGTTC CACTTCCTGC GCATCTACAA CCAGCTGCGG
    GCCTCGCTGC AGGACCTGAA GACCGTGGTC ATTGCCAAGA CCCCTGCAAC GGGGGGCGGG
    CCTCAGAGCC CCCTTGTGGA CGGCCAGCCT GCAGAGAGCA GGGCCAGCAG TGACCAGAAT
    CTCCAAGAAG CTCCAGCAGA GACTGCAGCC CTGGCCCTGG TGGAGGCCCC TGCTCTCACT
    CCAGGAGAGG CCTCAGCCCC AAGCCCGGCA AAGACCCCAG CTCCAGTGGA GGCCTCAGTG
    CCAGCTCTTG TTCCAGTGGA GGTCCCCGCG GAGGCCGAGG CCTCAGCAGA GATCCCAGTG
    GAGGCTCTGG CCCAAGCAGA GGCCCCTGCA CAGTACCCAA GTGAGCCCCT GATCCGGTCC
    ATGTCGGAGG ACAATCAGAT CCCGTCACAC CTGCCTGCCT GCCCGTCTCT CCGGCACATC
    GCCAGCCTGC GTGGACGGGC CGTGGTCGAG TTCTTCCACA GCAGCATTGC CGAGGTGGAA
    AATGAGGAGC TGCGGCACCT CCTGTGGTCA TCGGTGGTGT TCTACCAGAC GCCGGGGCTG
    GAGGTGACAG CCTGCGTGCT GCTCTCTACC AAGGCCGTGT ACTTCGTGCT GCACGACGGC
    CTCCGCCGCT ACTTCTCGGA GCCGCTGCAG GATTTCTGGC ATCAGAAAAA CACCGACTAC
    AACAACAGCC CCTTCCACAT CTCTCAGTGC TTTGTTTTGA AACTCAGCGA CCTGCAGGTA
    GTCAACGTTG GGCTGTTCGA CCAGTATTTC CGGCTAACGG GCTCCTGCCC GGCACAGGTG
    GTTACCTGCT TGACGCGGGA TAGCTACCTG ACACACTGCT TCCTCCAGCA CCTCATGGCT
    GTGCTCTCCC TGCTGGAACG CACACCCTCG CCTGAGCCTG TGGACAAAGA CTTCTATTCT
    GAGTTTGGAA ACAAGACCAC AGGGAAGATG GAAAACTATG AGCTGATCCA CTCGAGCCGC
    GTCAAGTTCA CCTACCCCAG CGAGGAGGAG GTCGGAGACC TGACGTTCAC TGTGGCCCAG
    AAGATGGCTG TGCCGGAGAA GGCGCCGGCC CTCAGCATCC TGCTGTGCGT GCAGGCCTTC
    CAGGTGGGCT CGCCGCAGCC GGGGCGCTGC AGGGGCTTGC TGCGCCCCAA GACGCTCCTG
    CTCACCAGCG CCGAGATCTT CCTCCTGGAC GAGGACTTCA TCCACTACCC GCTGCCCGAG
    TTCGCCAAAG AGCCGCCACA GAGGGACCGG TACCGGCTGG ACGACGGGCG CCGCGTCCGG
    GACCTGGACC GCGTGCTCAT GGGCTACCAG ACATACCCAC AGGCCCTCAC CCTGGTCTTT
    GATGATGTGC AGGGCCACGA CCTCATGGGC AGCGTCACCC TGGACCACTT CGGGGAGGTG
    CCAGGTGGCC TGGCCAGGGG TGGCCAGGGC CGTGAGGTCC AGTGGCAGGT GTTTGTCCCC
    AGCGCTGAGA GCCGAGAGAA GCTCATCTCG CTGCTGGCCC GTCAGTGGGA GGCCCTGTGT
    GGCCGGGAGC TGCCCGTGGA GCTCACCGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006044102.1
    CDS72..4604
    Translation

    Target ORF information:

    RefSeq Version XM_006044040.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis nischarin (NISCH), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006044040.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGGCGG CGGCGCGCAG CTTCGGGCCC GAGCGCGAGG CCGAGCCGGT CAAGACGGCG 
    CGCGTCGTGG GCTCTGAGCT CGTGGACACG TATACGGTTT ACATCATCCA GATCACTGAT
    GGGAGCCATG AGTGGACAGT CAAGCACCGC TACAGTGATT TCCACGACCT ACATGAAAAG
    CTTGTTGCGG AGAAGAAGAT TGATAGAAGT CTGCTTCCAC CCAAAAAGAT AATTGGGAAA
    AACTCCAGGA GCTTGGTGGA AAAGAGGGAG AAGGATCTCG AAATTTATCT CCAGACGCTC
    CTGGCCACCT TCCCTGACGT GGCCCCCAGA GTGCTGGCCC ACTTCTTGCA TTTCCACTTT
    TACGAGATTA ATGGCATCAC TGCAGCACTG GCTGAGGAAC TCTTCGAAAA AGGAGAACAG
    CTCCTGGGGG CTGGCGAGGT CTTCGCCATT GGACCCCTGC AGCTCTACGC AGTCACCGAG
    CAGCTGCAGC AGGGAAAGCC CACATGCGCC AGCGGGGATG CCAAGACTGA CCTGGGGCAC
    ATCCTGGACT TCACCTGTCG CCTTAAGTAC CTTAAGGTTT CTGGCACAAA AGGACCTTTT
    GGGACCAGCA ACATTCAGGA GCAGCTCCTG CCTTTTGATC TGTCCATATT CAAGTCTCTT
    CATCAGGTGG AGATAAGTCA CTGTGATGCC AGGCGCATCC GGGGGCTGGT TGCATCCAAG
    CCCACCTTGG CCACAATGAG CGTCCGTTTC TCGGCAACCT CCATGAAGGA AGTCCTCGTT
    CCTGAAGCCT CGGAGTTCGA TGAGTGGGAG CCAGCAGGCG CAGCCCTGGA GGGCCCCGTG
    ACTGCCGTCA TCCCCACATG GCAGGCTCTG ACCGCTCTGG ACTTGAGTCA CAATAGCATC
    TCTGAGATCG ATGATTCTGT GAAACTGATT CCAAAGATTG AGTTCCTGGA CCTGAGTCAC
    AATGGAGTGC TGGTCATGAA CAATTTGCAG CACCTGTACA ACCTTGTGCA CGTGGACCTG
    TCCTACAACA AGCTCTCCTC CTTGGAAGGG GTTCACACCA AACTGGGCAA CATCAAGACC
    CTGAACCTGG CAGGCAACCT CCTGAGGCAT CTGAGTGGCC TGCACAAGCT GTACTCCTTG
    GTCAACCTGG ATCTCAGCGA CAACAGAATC GAACAGATGG AAGAGGTCAG AAGCATCGGC
    AGCCTCCCGT GTCTGGAGCA CGTGGCTCTG CTGAACAACC CCCTGAGTAT CATTCCCGAC
    TACCGGACCA AGGTGCTGGC TCAGTTCGGA GAGCGGGCCT CGGAGGTCTG CCTGGACAAC
    ACAGTGACCA CAGAGAAAGA GTTGGACACA GTGGAAGTGC TAAAAGCGAT TCAGAAAGCC
    AAAGAGGTCA AGTCCAAATT AAGCAACCCT GAGAAGAAGG TCAGTGAGGA TTCTCGGCTC
    TCTGCTGCCC CCTGTGTCAG ACCCAGCAGC TCCCCACCCA GTGCGGCTCC CACCTCTGCC
    TCCCTGCCCC AGCCCATCCT CTCCAACCAA GGAATCATGT TTGTGCAAGA GGAGGCCCTG
    GCCAGCAGCC TCTCATCCAC CGACAGTCTG ACTCCTGAGG ACCGGTCCAT TGCGGGAGGA
    TGCTCTGATT CCTTGGAGTC CATCCCTGCG GGACAGGCAC CTTCTGATGA TTTAAGGGAT
    GTGCCAGGAG CTGTTGGCGG TGTGAGCCCC GAGCACGCGG AGCCAGAGGT CCAGGTGGTG
    CCTGGGTCCG GCCAGATAAT CTTCCTGCCC TTCACCTGCA TTGGCTACAC TGCCACCAAC
    CAGGACTTCA TCCAGCGCCT GAGCACGCTC ATCCGACAGG CTATTGAGCG GCAGCTCCCT
    GCTTGGATCG AGGCCGCCAA CCAGCGAGAG GAGGGCCAGG GGGAGCAGGG CGAGGAGGAG
    GAGGAGGAGG AGGACGTTGC TGAGAATCGC TACTTCGAAA TGGGGCCCCC AGATGCAGAG
    GAAGAGGAGG AGGGTGGGCC AGGCGAGGAA GAGGAAGAAG AGGAGGAGGA GGAGGAGGAG
    GCTGAGGAGG AGCGCCTGGC TCTGGAGTGG GCGCTGGGCG CAGACGAGGA CTTCCTGCTG
    GAGCATATCC GCATCCTCAA GGTGCTCTGG TGCTTCCTGA TCCACGTGCA GGGCAGCATC
    CGCCAGTTCG CCGCCTGCCT CGTGCTCACT GACTTCGGCA TCGCTGTCTT CGAGATCCCG
    CACCAGGAGT CGCGGGGCAG CAGCCAGCAC ATCCTTTCCT CACTGCGCTT CGTGTTCTGC
    TTCCCACACG GCGACCTCAC CGAGTTCGGC TTCCTCATGC CGGAGCTGTG CCTGGTGCTG
    AAGGTGCGGC ACAGCGAGAA CACACTCTTC ATCATCTCCG ACGCCGCCCA CCTGCACGAG
    TTCCACGCCG ACCTGCGCGC CTGCTTCGCC CCGCAGCACA TGGCCATGCT GTGCAGCCCC
    GTGCTCTACG GCAGCCACAC CAGCCTGCAG GAGTTCCTGC GCCAGCTGCT CACCTTCTAC
    AAGGTGGCAG GCGGCTGCCA GGAGCGCAGC CAGGGCTGCT TTCCCGTCTA CCTGGTCTAC
    AGTGACAAGC GCATGGTGCA GACAGCCGCC GGGGACTACT CGGGCAACAT CGAGTGGGCC
    AGCTGCACCC TCTGCTCGGC TGTGCGGCGC TCCTGCTGTG CGCCCTCCGA GGCCGTCAAG
    TCCGCCGCCA TCCCCTACTG GCTGTTGCTC ACGCCCCAGC ACCTCAACGT CATCAAGGCC
    GACTTCAACC CCATGCCCAA CCGGGGCACC CACAACTGCC GCAACCGCAA CAGCTTCAAG
    CTCAGCCGCG TGCCTCTCTC CACTGTGCTG CTGGACCCCA CGCGCAGCTG CACCCAGCCA
    CGGGGCGCCT TCGCAGATGG CCACGTGCTT GAGCTGCTCG TGGGCTACCG CTTCGTCACC
    GCCATCTTCG TGCTGCCCCA TGAGAAGTTC CACTTCCTGC GCATCTACAA CCAGCTGCGG
    GCCTCGCTGC AGGACCTGAA GACCGTGGTC ATTGCCAAGA CCCCTGCAAC GGGGGGCGGG
    CCTCAGAGCC CCCTTGTGGA CGGCCAGCCT GCAGAGAGCA GGGCCAGCAG TGACCAGAAT
    CTCCAAGAAG CTCCAGCAGA GACTGCAGCC CTGGCCCTGG TGGAGGCCCC TGCTCTCACT
    CCAGGAGAGG CCTCAGCCCC AAGCCCGGCA AAGACCCCAG CTCCAGTGGA GGCCTCAGTG
    CCAGCTCTTG TTCCAGTGGA GGTCCCCGCG GAGGCCGAGG CCTCAGCAGA GATCCCAGTG
    GAGGCTCTGG CCCAAGCAGA GGCCCCTGCA CAGTACCCAA GTGAGCCCCT GATCCGGTCC
    ATGTCGGAGG ACAATCAGAT CCCGTCACAC CTGCCTGCCT GCCCGTCTCT CCGGCACATC
    GCCAGCCTGC GTGGACGGGC CGTGGTCGAG TTCTTCCACA GCAGCATTGC CGAGGTGGAA
    AATGAGGAGC TGCGGCACCT CCTGTGGTCA TCGGTGGTGT TCTACCAGAC GCCGGGGCTG
    GAGGTGACAG CCTGCGTGCT GCTCTCTACC AAGGCCGTGT ACTTCGTGCT GCACGACGGC
    CTCCGCCGCT ACTTCTCGGA GCCGCTGCAG GATTTCTGGC ATCAGAAAAA CACCGACTAC
    AACAACAGCC CCTTCCACAT CTCTCAGTGC TTTGTTTTGA AACTCAGCGA CCTGCAGGTA
    GTCAACGTTG GGCTGTTCGA CCAGTATTTC CGGCTAACGG GCTCCTGCCC GGCACAGGTG
    GTTACCTGCT TGACGCGGGA TAGCTACCTG ACACACTGCT TCCTCCAGCA CCTCATGGCT
    GTGCTCTCCC TGCTGGAACG CACACCCTCG CCTGAGCCTG TGGACAAAGA CTTCTATTCT
    GAGTTTGGAA ACAAGACCAC AGGGAAGATG GAAAACTATG AGCTGATCCA CTCGAGCCGC
    GTCAAGTTCA CCTACCCCAG CGAGGAGGAG GTCGGAGACC TGACGTTCAC TGTGGCCCAG
    AAGATGGCTG TGCCGGAGAA GGCGCCGGCC CTCAGCATCC TGCTGTGCGT GCAGGCCTTC
    CAGGTGGGCT CGCCGCAGCC GGGGCGCTGC AGGGGCTTGC TGCGCCCCAA GACGCTCCTG
    CTCACCAGCG CCGAGATCTT CCTCCTGGAC GAGGACTTCA TCCACTACCC GCTGCCCGAG
    TTCGCCAAAG AGCCGCCACA GAGGGACCGG TACCGGCTGG ACGACGGGCG CCGCGTCCGG
    GACCTGGACC GCGTGCTCAT GGGCTACCAG ACATACCCAC AGGCCCTCAC CCTGGTCTTT
    GATGATGTGC AGGGCCACGA CCTCATGGGC AGCGTCACCC TGGACCACTT CGGGGAGGTG
    CCAGGTGGCC TGGCCAGGGG TGGCCAGGGC CGTGAGGTCC AGTGGCAGGT GTTTGTCCCC
    AGCGCTGAGA GCCGAGAGAA GCTCATCTCG CTGCTGGCCC GTCAGTGGGA GGCCCTGTGT
    GGCCGGGAGC TGCCCGTGGA GCTCACCGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.