HECTD1 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following HECTD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECTD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu13695 XM_006056604.1
Latest version!
Bubalus bubalis HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBu13696 XM_006056605.1
Latest version!
Bubalus bubalis HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBu13697 XM_006056606.1
Latest version!
Bubalus bubalis HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBu13698 XM_006056607.1
Latest version!
Bubalus bubalis HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBu13699 XM_006056608.1
Latest version!
Bubalus bubalis HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBu56860 XM_025271334.1
Latest version!
Bubalus bubalis HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBu56860 XM_006056604.2
Latest version!
Bubalus bubalis HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBu56861 XM_025271335.1
Latest version!
Bubalus bubalis HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBu56862 XM_025271336.1
Latest version!
Bubalus bubalis HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBu56863 XM_025271337.1
Latest version!
Bubalus bubalis HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBu56864 XM_025271338.1
Latest version!
Bubalus bubalis HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu13695
    Clone ID Related Accession (Same CDS sequence) XM_006056604.1
    Accession Version XM_006056604.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784766.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CACAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCGGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACCA AGGGGGGCCG CCGGAGAACA
    TGGGACGATG ATTATGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCATTGG CATTGGCAAG TGGAGCTCTG
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAAAA CGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT CTGGGGCCCA CATTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGTGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTACGTG
    CAGAGATCAT GTGGACTGTT CACAGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGATGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCGCCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG CCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTAA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006056666.1
    CDS357..8189
    Translation

    Target ORF information:

    RefSeq Version XM_006056604.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006056604.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CACAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCTGG AGCGGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACCA AGGGGGGCCG CCGGAGAACA
    TGGGACGATG ATTATGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCATTGG CATTGGCAAG TGGAGCTCTG
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAAAA CGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT CTGGGGCCCA CATTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGTGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTACGTG
    CAGAGATCAT GTGGACTGTT CACAGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGATGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCGCCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG CCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTAA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu13696
    Clone ID Related Accession (Same CDS sequence) XM_006056605.1
    Accession Version XM_006056605.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784766.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCCCTGAT
    AGTTCTGCTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTA ACTAATAAAG AAGCAGCTTC ACAACGGCCC
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC CGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CCAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCCGGTAC TACATCGACA
    GTGACTATGT CAACATCCAG TGTTACTAGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAGTCTTT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAGCC AGACGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCGT CCCTACAGCG CCGAGCTGGC TCCCGCTCTG ATGTAACACA CCATGCTGTC
    ACCTCACAGC TACCACAGGT CCCAGCTGGA GCGGGGAGCC GACCTATTGG GGAGCAGGAA
    GAGGAAGAGT ACGAAACCAA GGGGGGCCGC CGGAGAACAT GGGACGATGA TTATGTGCTC
    AAGCGGCAGT TTTCAGCTTT GGTTCCTGCT TTTGATCCTA GACCTGGTCG AACTAATGTC
    CAGCAGACAA CTGATCTAGA AATTCCACCC CCAGGAACAC CTCATTCAGA GCTTTTGGAA
    GAAGTTGAAT GTACTCCATC ACCTCGATTA GCCCTCACTT TAAAAGTAAC AGGTCTTGGA
    ACAACTCGTG AAGTTGAATT ACCACTCACT AATTTCAGAT CAACCATCTT TTACTATGTA
    CAGAAATTGC TTCAGTTGTC ATGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCAA CATACACAAT TATGTACAGA GAAATGAAGG ATTCTGACAA AGAAAAGGAA
    AATGGAAAAA TGGGTTGTTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGATGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCCGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC
    ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGGTTAAA CCAGGGGGCC
    ATTTCTACTC TTCAAAGTAG TGATATTCTG AATTTGACAA AAGAACAACC TCAGGCCAAA
    GCAGGCAATG GGCAGAATTC ATGTGGCGTG GAAGACGTCC TTCAGCTCCT GCGTATTCTG
    TATATAGTTG CAAGTGACCC TTACTCAAGA ATATCCCAGG AAGAAGGTGA TGAGCAGCCT
    CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TCACAACAAA AATTTTGCAG
    CAAATTGAGG AACCATTGGC ATTGGCAAGT GGAGCTCTGC CAGACTGGTG TGAACAGTTA
    ACCAGCAAGT GTCCTTTTCT AATACCGTTC GAAACTAGAC AGCTTTATTT CACATGTACA
    GCATTTGGTG CATCAAGAGC AATTGTGTGG CTACAAAACC GACGTGAGGC CACTGTGGAA
    AGAACAAGGA CCACAAGTAG TGTGAGGCGA GATGATCCTG GAGAGTTCCG TGTTGGTCGT
    CTCAAGCACG AAAGAGTAAA AGTTCCACGT GGTGAATCAC TGATGGAGTG GGCCGAAAAC
    GTCATGCAAA TACACGCAGA TCGGAAATCA GTGCTTGAGG TTGAATTTTT AGGAGAAGAA
    GGAACTGGTC TGGGGCCCAC ATTAGAGTTC TATGCTCTGG TAGCAGCAGA ATTCCAGAGA
    ACTGACTTGG GTGCTTGGCT TTGTGATGAC AACTTTCCAG ATGATGAATC TCGTCATGTC
    GATCTTGGAG GTGGATTGAA ACCTCCCGGA TACTACGTGC AGAGATCATG TGGACTGTTC
    ACAGCTCCAT TTCCACAAGA CAGTGACGAG CTTGAAAGGA TCACAAAACT CTTTCATTTC
    CTTGGAATTT TCTTGGCCAA ATGCATTCAA GACAATAGAC TTGTGGACTT ACCTATTTCC
    AAACCTTTCT TTAAACTTAT GTGTATGGGG GACATTAAAA GTAATATGAG CAAACTGATT
    TATGAGTCAC GAGGCGACAG AGACCTACAC TGCACTGAAA GTCAGTCTGA AGCCTCTACA
    GAAGAAGGGC ATGACTCACT CTCAGTAGGA AGCTTTGAGG AGGATTCAAA ATCAGAATTC
    ATTCTAGATC CCCCCAAACC AAAACCGCCA GCTTGGTTTA ATGGAATTTT AACTTGGGAA
    GACTTTGAAC TAGTAAACCC ACACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCC
    ATCAAGAGGC GCCAGATTTT AAGCAACAAA GGTCTTTCTG AAGATGAGAA GAACACAAAA
    TTACAAGAAC TAGTGCTGAA GAATCCATCA GGTTCTGGGC CTCCACTTAG CATAGAGGAT
    TTAGGTTTAA ATTTCCAGTT TTGCCCTTCC TCAAGAATAT ATGGTTTTAC AGCTGTGGAT
    CTCAAGCCAA GTGGTGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTTTGCAT GCATACGGGT ATTCAGAAAC AGATGGAAGC CTTCAGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA AGAGGTCCAA
    ATGATTCTTT GTGGAAATCA GTCGCCATCC TGGGCAGCAG AGGATATTAT TAATTATACT
    GAACCTAAGT TGGGTTATAC ACGTGACAGC CCTGGCTTCC TTAGGTTCGT GCGGGTTTTA
    TGTGGTATGT CTTCAGACGA GAGGAAAGCA TTTCTGCAGT TTACCACTGG TTGTTCAACT
    CTGCCTCCAG GGGGACTGGC TAACTTGCAT CCCAGGCTCA CAGTTGTACG CAAGGTTGAT
    GCTACTGATG CAAGCTACCC ATCAGTGAAC ACATGTGTGC ATTATCTAAA GTTGCCAGAA
    TACTCTTCGG AGGAGATCAT GAGAGAGCGT CTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006056667.1
    CDS357..8048
    Translation

    Target ORF information:

    RefSeq Version XM_006056605.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006056605.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCCCTGAT
    AGTTCTGCTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTA ACTAATAAAG AAGCAGCTTC ACAACGGCCC
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC CGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CCAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCCGGTAC TACATCGACA
    GTGACTATGT CAACATCCAG TGTTACTAGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAGTCTTT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAGCC AGACGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCGT CCCTACAGCG CCGAGCTGGC TCCCGCTCTG ATGTAACACA CCATGCTGTC
    ACCTCACAGC TACCACAGGT CCCAGCTGGA GCGGGGAGCC GACCTATTGG GGAGCAGGAA
    GAGGAAGAGT ACGAAACCAA GGGGGGCCGC CGGAGAACAT GGGACGATGA TTATGTGCTC
    AAGCGGCAGT TTTCAGCTTT GGTTCCTGCT TTTGATCCTA GACCTGGTCG AACTAATGTC
    CAGCAGACAA CTGATCTAGA AATTCCACCC CCAGGAACAC CTCATTCAGA GCTTTTGGAA
    GAAGTTGAAT GTACTCCATC ACCTCGATTA GCCCTCACTT TAAAAGTAAC AGGTCTTGGA
    ACAACTCGTG AAGTTGAATT ACCACTCACT AATTTCAGAT CAACCATCTT TTACTATGTA
    CAGAAATTGC TTCAGTTGTC ATGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCAA CATACACAAT TATGTACAGA GAAATGAAGG ATTCTGACAA AGAAAAGGAA
    AATGGAAAAA TGGGTTGTTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGATGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCCGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC
    ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGGTTAAA CCAGGGGGCC
    ATTTCTACTC TTCAAAGTAG TGATATTCTG AATTTGACAA AAGAACAACC TCAGGCCAAA
    GCAGGCAATG GGCAGAATTC ATGTGGCGTG GAAGACGTCC TTCAGCTCCT GCGTATTCTG
    TATATAGTTG CAAGTGACCC TTACTCAAGA ATATCCCAGG AAGAAGGTGA TGAGCAGCCT
    CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TCACAACAAA AATTTTGCAG
    CAAATTGAGG AACCATTGGC ATTGGCAAGT GGAGCTCTGC CAGACTGGTG TGAACAGTTA
    ACCAGCAAGT GTCCTTTTCT AATACCGTTC GAAACTAGAC AGCTTTATTT CACATGTACA
    GCATTTGGTG CATCAAGAGC AATTGTGTGG CTACAAAACC GACGTGAGGC CACTGTGGAA
    AGAACAAGGA CCACAAGTAG TGTGAGGCGA GATGATCCTG GAGAGTTCCG TGTTGGTCGT
    CTCAAGCACG AAAGAGTAAA AGTTCCACGT GGTGAATCAC TGATGGAGTG GGCCGAAAAC
    GTCATGCAAA TACACGCAGA TCGGAAATCA GTGCTTGAGG TTGAATTTTT AGGAGAAGAA
    GGAACTGGTC TGGGGCCCAC ATTAGAGTTC TATGCTCTGG TAGCAGCAGA ATTCCAGAGA
    ACTGACTTGG GTGCTTGGCT TTGTGATGAC AACTTTCCAG ATGATGAATC TCGTCATGTC
    GATCTTGGAG GTGGATTGAA ACCTCCCGGA TACTACGTGC AGAGATCATG TGGACTGTTC
    ACAGCTCCAT TTCCACAAGA CAGTGACGAG CTTGAAAGGA TCACAAAACT CTTTCATTTC
    CTTGGAATTT TCTTGGCCAA ATGCATTCAA GACAATAGAC TTGTGGACTT ACCTATTTCC
    AAACCTTTCT TTAAACTTAT GTGTATGGGG GACATTAAAA GTAATATGAG CAAACTGATT
    TATGAGTCAC GAGGCGACAG AGACCTACAC TGCACTGAAA GTCAGTCTGA AGCCTCTACA
    GAAGAAGGGC ATGACTCACT CTCAGTAGGA AGCTTTGAGG AGGATTCAAA ATCAGAATTC
    ATTCTAGATC CCCCCAAACC AAAACCGCCA GCTTGGTTTA ATGGAATTTT AACTTGGGAA
    GACTTTGAAC TAGTAAACCC ACACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCC
    ATCAAGAGGC GCCAGATTTT AAGCAACAAA GGTCTTTCTG AAGATGAGAA GAACACAAAA
    TTACAAGAAC TAGTGCTGAA GAATCCATCA GGTTCTGGGC CTCCACTTAG CATAGAGGAT
    TTAGGTTTAA ATTTCCAGTT TTGCCCTTCC TCAAGAATAT ATGGTTTTAC AGCTGTGGAT
    CTCAAGCCAA GTGGTGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTTTGCAT GCATACGGGT ATTCAGAAAC AGATGGAAGC CTTCAGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA AGAGGTCCAA
    ATGATTCTTT GTGGAAATCA GTCGCCATCC TGGGCAGCAG AGGATATTAT TAATTATACT
    GAACCTAAGT TGGGTTATAC ACGTGACAGC CCTGGCTTCC TTAGGTTCGT GCGGGTTTTA
    TGTGGTATGT CTTCAGACGA GAGGAAAGCA TTTCTGCAGT TTACCACTGG TTGTTCAACT
    CTGCCTCCAG GGGGACTGGC TAACTTGCAT CCCAGGCTCA CAGTTGTACG CAAGGTTGAT
    GCTACTGATG CAAGCTACCC ATCAGTGAAC ACATGTGTGC ATTATCTAAA GTTGCCAGAA
    TACTCTTCGG AGGAGATCAT GAGAGAGCGT CTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu13697
    Clone ID Related Accession (Same CDS sequence) XM_006056606.1
    Accession Version XM_006056606.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784766.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCCCTGAT
    AGTTCTGCTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTA ACTAATAAAG AAGCAGCTTC ACAACGGCCC
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC CGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CCAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCCGGTAC TACATCGACA
    GTGACTATGT CAACATCCAG TGTTACTAGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAGTCTTT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAGCC AGACGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGAT TCTGAGACGT
    CCGTCCCTAC AGCGCCGAGC TGGCTCCCGC TCTGATGTAA CACACCATGC TGTCACCTCA
    CAGCTACCAC AGGTCCCAGC TGGAGCGGGG AGCCGACCTA TTGGGGAGCA GGAAGAGGAA
    GAGTACGAAA CCAAGGGGGG CCGCCGGAGA ACATGGGACG ATGATTATGT GCTCAAGCGG
    CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT CCTAGACCTG GTCGAACTAA TGTCCAGCAG
    ACAACTGATC TAGAAATTCC ACCCCCAGGA ACACCTCATT CAGAGCTTTT GGAAGAAGTT
    GAATGTACTC CATCACCTCG ATTAGCCCTC ACTTTAAAAG TAACAGGTCT TGGAACAACT
    CGTGAAGTTG AATTACCACT CACTAATTTC AGATCAACCA TCTTTTACTA TGTACAGAAA
    TTGCTTCAGT TGTCATGTAA TGGCAATGTG AAATCAGATA AACTTAGGCG TATTTGGGAG
    CCAACATACA CAATTATGTA CAGAGAAATG AAGGATTCTG ACAAAGAAAA GGAAAATGGA
    AAAATGGGTT GTTGGTCTAT AGAGCATGTG GAACAGTACC TTGGCACTGA TGAATTACCA
    AAGAATGACT TGATAACCTA CCTGCAGAAG AATGCAGACG CCGCTTTCCT GCGCCACTGG
    AAATTAACTG GCACTAATAA AAGTATTAGG AAAAACAGAA ATTGTTCTCA GCTCATAGCT
    GCATATAAGG ATTTTTGTGA GCATGGAACA AAGTCTGGGT TAAACCAGGG GGCCATTTCT
    ACTCTTCAAA GTAGTGATAT TCTGAATTTG ACAAAAGAAC AACCTCAGGC CAAAGCAGGC
    AATGGGCAGA ATTCATGTGG CGTGGAAGAC GTCCTTCAGC TCCTGCGTAT TCTGTATATA
    GTTGCAAGTG ACCCTTACTC AAGAATATCC CAGGAAGAAG GTGATGAGCA GCCTCAGTTT
    ACTTTTCCAC CAGATGAATT CACTAGCAAA AAAATCACAA CAAAAATTTT GCAGCAAATT
    GAGGAACCAT TGGCATTGGC AAGTGGAGCT CTGCCAGACT GGTGTGAACA GTTAACCAGC
    AAGTGTCCTT TTCTAATACC GTTCGAAACT AGACAGCTTT ATTTCACATG TACAGCATTT
    GGTGCATCAA GAGCAATTGT GTGGCTACAA AACCGACGTG AGGCCACTGT GGAAAGAACA
    AGGACCACAA GTAGTGTGAG GCGAGATGAT CCTGGAGAGT TCCGTGTTGG TCGTCTCAAG
    CACGAAAGAG TAAAAGTTCC ACGTGGTGAA TCACTGATGG AGTGGGCCGA AAACGTCATG
    CAAATACACG CAGATCGGAA ATCAGTGCTT GAGGTTGAAT TTTTAGGAGA AGAAGGAACT
    GGTCTGGGGC CCACATTAGA GTTCTATGCT CTGGTAGCAG CAGAATTCCA GAGAACTGAC
    TTGGGTGCTT GGCTTTGTGA TGACAACTTT CCAGATGATG AATCTCGTCA TGTCGATCTT
    GGAGGTGGAT TGAAACCTCC CGGATACTAC GTGCAGAGAT CATGTGGACT GTTCACAGCT
    CCATTTCCAC AAGACAGTGA CGAGCTTGAA AGGATCACAA AACTCTTTCA TTTCCTTGGA
    ATTTTCTTGG CCAAATGCAT TCAAGACAAT AGACTTGTGG ACTTACCTAT TTCCAAACCT
    TTCTTTAAAC TTATGTGTAT GGGGGACATT AAAAGTAATA TGAGCAAACT GATTTATGAG
    TCACGAGGCG ACAGAGACCT ACACTGCACT GAAAGTCAGT CTGAAGCCTC TACAGAAGAA
    GGGCATGACT CACTCTCAGT AGGAAGCTTT GAGGAGGATT CAAAATCAGA ATTCATTCTA
    GATCCCCCCA AACCAAAACC GCCAGCTTGG TTTAATGGAA TTTTAACTTG GGAAGACTTT
    GAACTAGTAA ACCCACACAG AGCCAGATTT TTAAAAGAAA TTAAAGACCT TGCCATCAAG
    AGGCGCCAGA TTTTAAGCAA CAAAGGTCTT TCTGAAGATG AGAAGAACAC AAAATTACAA
    GAACTAGTGC TGAAGAATCC ATCAGGTTCT GGGCCTCCAC TTAGCATAGA GGATTTAGGT
    TTAAATTTCC AGTTTTGCCC TTCCTCAAGA ATATATGGTT TTACAGCTGT GGATCTCAAG
    CCAAGTGGTG AAGATGAGAT GATAACAATG GATAATGCAG AAGAATATGT GGATTTGATG
    TTTGACTTTT GCATGCATAC GGGTATTCAG AAACAGATGG AAGCCTTCAG AGATGGGTTT
    AATAAAGTTT TTCCAATGGA GAAATTAAGT TCCTTCAGCC ATGAAGAGGT CCAAATGATT
    CTTTGTGGAA ATCAGTCGCC ATCCTGGGCA GCAGAGGATA TTATTAATTA TACTGAACCT
    AAGTTGGGTT ATACACGTGA CAGCCCTGGC TTCCTTAGGT TCGTGCGGGT TTTATGTGGT
    ATGTCTTCAG ACGAGAGGAA AGCATTTCTG CAGTTTACCA CTGGTTGTTC AACTCTGCCT
    CCAGGGGGAC TGGCTAACTT GCATCCCAGG CTCACAGTTG TACGCAAGGT TGATGCTACT
    GATGCAAGCT ACCCATCAGT GAACACATGT GTGCATTATC TAAAGTTGCC AGAATACTCT
    TCGGAGGAGA TCATGAGAGA GCGTCTGCTA GCTGCTACAA TGGAGAAAGG CTTTCATCTC
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006056668.1
    CDS357..8042
    Translation

    Target ORF information:

    RefSeq Version XM_006056606.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006056606.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCCCTGAT
    AGTTCTGCTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTA ACTAATAAAG AAGCAGCTTC ACAACGGCCC
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC CGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CCAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCCGGTAC TACATCGACA
    GTGACTATGT CAACATCCAG TGTTACTAGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAGTCTTT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAGCC AGACGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGAT TCTGAGACGT
    CCGTCCCTAC AGCGCCGAGC TGGCTCCCGC TCTGATGTAA CACACCATGC TGTCACCTCA
    CAGCTACCAC AGGTCCCAGC TGGAGCGGGG AGCCGACCTA TTGGGGAGCA GGAAGAGGAA
    GAGTACGAAA CCAAGGGGGG CCGCCGGAGA ACATGGGACG ATGATTATGT GCTCAAGCGG
    CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT CCTAGACCTG GTCGAACTAA TGTCCAGCAG
    ACAACTGATC TAGAAATTCC ACCCCCAGGA ACACCTCATT CAGAGCTTTT GGAAGAAGTT
    GAATGTACTC CATCACCTCG ATTAGCCCTC ACTTTAAAAG TAACAGGTCT TGGAACAACT
    CGTGAAGTTG AATTACCACT CACTAATTTC AGATCAACCA TCTTTTACTA TGTACAGAAA
    TTGCTTCAGT TGTCATGTAA TGGCAATGTG AAATCAGATA AACTTAGGCG TATTTGGGAG
    CCAACATACA CAATTATGTA CAGAGAAATG AAGGATTCTG ACAAAGAAAA GGAAAATGGA
    AAAATGGGTT GTTGGTCTAT AGAGCATGTG GAACAGTACC TTGGCACTGA TGAATTACCA
    AAGAATGACT TGATAACCTA CCTGCAGAAG AATGCAGACG CCGCTTTCCT GCGCCACTGG
    AAATTAACTG GCACTAATAA AAGTATTAGG AAAAACAGAA ATTGTTCTCA GCTCATAGCT
    GCATATAAGG ATTTTTGTGA GCATGGAACA AAGTCTGGGT TAAACCAGGG GGCCATTTCT
    ACTCTTCAAA GTAGTGATAT TCTGAATTTG ACAAAAGAAC AACCTCAGGC CAAAGCAGGC
    AATGGGCAGA ATTCATGTGG CGTGGAAGAC GTCCTTCAGC TCCTGCGTAT TCTGTATATA
    GTTGCAAGTG ACCCTTACTC AAGAATATCC CAGGAAGAAG GTGATGAGCA GCCTCAGTTT
    ACTTTTCCAC CAGATGAATT CACTAGCAAA AAAATCACAA CAAAAATTTT GCAGCAAATT
    GAGGAACCAT TGGCATTGGC AAGTGGAGCT CTGCCAGACT GGTGTGAACA GTTAACCAGC
    AAGTGTCCTT TTCTAATACC GTTCGAAACT AGACAGCTTT ATTTCACATG TACAGCATTT
    GGTGCATCAA GAGCAATTGT GTGGCTACAA AACCGACGTG AGGCCACTGT GGAAAGAACA
    AGGACCACAA GTAGTGTGAG GCGAGATGAT CCTGGAGAGT TCCGTGTTGG TCGTCTCAAG
    CACGAAAGAG TAAAAGTTCC ACGTGGTGAA TCACTGATGG AGTGGGCCGA AAACGTCATG
    CAAATACACG CAGATCGGAA ATCAGTGCTT GAGGTTGAAT TTTTAGGAGA AGAAGGAACT
    GGTCTGGGGC CCACATTAGA GTTCTATGCT CTGGTAGCAG CAGAATTCCA GAGAACTGAC
    TTGGGTGCTT GGCTTTGTGA TGACAACTTT CCAGATGATG AATCTCGTCA TGTCGATCTT
    GGAGGTGGAT TGAAACCTCC CGGATACTAC GTGCAGAGAT CATGTGGACT GTTCACAGCT
    CCATTTCCAC AAGACAGTGA CGAGCTTGAA AGGATCACAA AACTCTTTCA TTTCCTTGGA
    ATTTTCTTGG CCAAATGCAT TCAAGACAAT AGACTTGTGG ACTTACCTAT TTCCAAACCT
    TTCTTTAAAC TTATGTGTAT GGGGGACATT AAAAGTAATA TGAGCAAACT GATTTATGAG
    TCACGAGGCG ACAGAGACCT ACACTGCACT GAAAGTCAGT CTGAAGCCTC TACAGAAGAA
    GGGCATGACT CACTCTCAGT AGGAAGCTTT GAGGAGGATT CAAAATCAGA ATTCATTCTA
    GATCCCCCCA AACCAAAACC GCCAGCTTGG TTTAATGGAA TTTTAACTTG GGAAGACTTT
    GAACTAGTAA ACCCACACAG AGCCAGATTT TTAAAAGAAA TTAAAGACCT TGCCATCAAG
    AGGCGCCAGA TTTTAAGCAA CAAAGGTCTT TCTGAAGATG AGAAGAACAC AAAATTACAA
    GAACTAGTGC TGAAGAATCC ATCAGGTTCT GGGCCTCCAC TTAGCATAGA GGATTTAGGT
    TTAAATTTCC AGTTTTGCCC TTCCTCAAGA ATATATGGTT TTACAGCTGT GGATCTCAAG
    CCAAGTGGTG AAGATGAGAT GATAACAATG GATAATGCAG AAGAATATGT GGATTTGATG
    TTTGACTTTT GCATGCATAC GGGTATTCAG AAACAGATGG AAGCCTTCAG AGATGGGTTT
    AATAAAGTTT TTCCAATGGA GAAATTAAGT TCCTTCAGCC ATGAAGAGGT CCAAATGATT
    CTTTGTGGAA ATCAGTCGCC ATCCTGGGCA GCAGAGGATA TTATTAATTA TACTGAACCT
    AAGTTGGGTT ATACACGTGA CAGCCCTGGC TTCCTTAGGT TCGTGCGGGT TTTATGTGGT
    ATGTCTTCAG ACGAGAGGAA AGCATTTCTG CAGTTTACCA CTGGTTGTTC AACTCTGCCT
    CCAGGGGGAC TGGCTAACTT GCATCCCAGG CTCACAGTTG TACGCAAGGT TGATGCTACT
    GATGCAAGCT ACCCATCAGT GAACACATGT GTGCATTATC TAAAGTTGCC AGAATACTCT
    TCGGAGGAGA TCATGAGAGA GCGTCTGCTA GCTGCTACAA TGGAGAAAGG CTTTCATCTC
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu13698
    Clone ID Related Accession (Same CDS sequence) XM_006056607.1
    Accession Version XM_006056607.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7509bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784766.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCCCTGAT
    AGTTCTGCTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTA ACTAATAAAG AAGCAGCTTC ACAACGGCCC
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC CGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CCAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCCGGTAC TACATCGACA
    GTGACTATGT CAACATCCAG TGTTACTAGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAGTCTTT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAGCC AGACGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCGT CCCTACAGCG CCGAGCTGGC TCCCGCTCTG ATGTAACACA CCATGCTGTC
    ACCTCACAGC TACCACAGGT CCCAGCTGGA GCGGGGAGCC GACCTATTGG GGAGCAGGAA
    GAGGAAGAGT ACGAAACCAA GGGGGGCCGC CGGAGAACAT GGGACGATGA TTATGTGCTC
    AAGCGGCAGT TTTCAGCTTT GGTTCCTGCT TTTGATCCTA GACCTGGTCG AACTAATGTC
    CAGCAGACAA CTGATCTAGA AATTCCACCC CCAGGAACAC CTCATTCAGA GCTTTTGGAA
    GAAGTTGAAT GTACTCCATC ACCTCGATTA GCCCTCACTT TAAAAGTAAC AGGTCTTGGA
    ACAACTCGTG AAGTTGAATT ACCACTCACT AATTTCAGAT CAACCATCTT TTACTATGTA
    CAGAAATTGC TTCAGTTGTC ATGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCAA CATACACAAT TATGTACAGA GAAATGAAGG ATTCTGACAA AGAAAAGGAA
    AATGGAAAAA TGGATTTTTG TGAGCATGGA ACAAAGTCTG GGTTAAACCA GGGGGCCATT
    TCTACTCTTC AAAGTAGTGA TATTCTGAAT TTGACAAAAG AACAACCTCA GGCCAAAGCA
    GGCAATGGGC AGAATTCATG TGGCGTGGAA GACGTCCTTC AGCTCCTGCG TATTCTGTAT
    ATAGTTGCAA GTGACCCTTA CTCAAGAATA TCCCAGGAAG AAGGTGATGA GCAGCCTCAG
    TTTACTTTTC CACCAGATGA ATTCACTAGC AAAAAAATCA CAACAAAAAT TTTGCAGCAA
    ATTGAGGAAC CATTGGCATT GGCAAGTGGA GCTCTGCCAG ACTGGTGTGA ACAGTTAACC
    AGCAAGTGTC CTTTTCTAAT ACCGTTCGAA ACTAGACAGC TTTATTTCAC ATGTACAGCA
    TTTGGTGCAT CAAGAGCAAT TGTGTGGCTA CAAAACCGAC GTGAGGCCAC TGTGGAAAGA
    ACAAGGACCA CAAGTAGTGT GAGGCGAGAT GATCCTGGAG AGTTCCGTGT TGGTCGTCTC
    AAGCACGAAA GAGTAAAAGT TCCACGTGGT GAATCACTGA TGGAGTGGGC CGAAAACGTC
    ATGCAAATAC ACGCAGATCG GAAATCAGTG CTTGAGGTTG AATTTTTAGG AGAAGAAGGA
    ACTGGTCTGG GGCCCACATT AGAGTTCTAT GCTCTGGTAG CAGCAGAATT CCAGAGAACT
    GACTTGGGTG CTTGGCTTTG TGATGACAAC TTTCCAGATG ATGAATCTCG TCATGTCGAT
    CTTGGAGGTG GATTGAAACC TCCCGGATAC TACGTGCAGA GATCATGTGG ACTGTTCACA
    GCTCCATTTC CACAAGACAG TGACGAGCTT GAAAGGATCA CAAAACTCTT TCATTTCCTT
    GGAATTTTCT TGGCCAAATG CATTCAAGAC AATAGACTTG TGGACTTACC TATTTCCAAA
    CCTTTCTTTA AACTTATGTG TATGGGGGAC ATTAAAAGTA ATATGAGCAA ACTGATTTAT
    GAGTCACGAG GCGACAGAGA CCTACACTGC ACTGAAAGTC AGTCTGAAGC CTCTACAGAA
    GAAGGGCATG ACTCACTCTC AGTAGGAAGC TTTGAGGAGG ATTCAAAATC AGAATTCATT
    CTAGATCCCC CCAAACCAAA ACCGCCAGCT TGGTTTAATG GAATTTTAAC TTGGGAAGAC
    TTTGAACTAG TAAACCCACA CAGAGCCAGA TTTTTAAAAG AAATTAAAGA CCTTGCCATC
    AAGAGGCGCC AGATTTTAAG CAACAAAGGT CTTTCTGAAG ATGAGAAGAA CACAAAATTA
    CAAGAACTAG TGCTGAAGAA TCCATCAGGT TCTGGGCCTC CACTTAGCAT AGAGGATTTA
    GGTTTAAATT TCCAGTTTTG CCCTTCCTCA AGAATATATG GTTTTACAGC TGTGGATCTC
    AAGCCAAGTG GTGAAGATGA GATGATAACA ATGGATAATG CAGAAGAATA TGTGGATTTG
    ATGTTTGACT TTTGCATGCA TACGGGTATT CAGAAACAGA TGGAAGCCTT CAGAGATGGG
    TTTAATAAAG TTTTTCCAAT GGAGAAATTA AGTTCCTTCA GCCATGAAGA GGTCCAAATG
    ATTCTTTGTG GAAATCAGTC GCCATCCTGG GCAGCAGAGG ATATTATTAA TTATACTGAA
    CCTAAGTTGG GTTATACACG TGACAGCCCT GGCTTCCTTA GGTTCGTGCG GGTTTTATGT
    GGTATGTCTT CAGACGAGAG GAAAGCATTT CTGCAGTTTA CCACTGGTTG TTCAACTCTG
    CCTCCAGGGG GACTGGCTAA CTTGCATCCC AGGCTCACAG TTGTACGCAA GGTTGATGCT
    ACTGATGCAA GCTACCCATC AGTGAACACA TGTGTGCATT ATCTAAAGTT GCCAGAATAC
    TCTTCGGAGG AGATCATGAG AGAGCGTCTG CTAGCTGCTA CAATGGAGAA AGGCTTTCAT
    CTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006056669.1
    CDS357..7865
    Translation

    Target ORF information:

    RefSeq Version XM_006056607.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006056607.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCCCTGAT
    AGTTCTGCTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTA ACTAATAAAG AAGCAGCTTC ACAACGGCCC
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC CGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CCAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCCGGTAC TACATCGACA
    GTGACTATGT CAACATCCAG TGTTACTAGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAGTCTTT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAGCC AGACGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCGT CCCTACAGCG CCGAGCTGGC TCCCGCTCTG ATGTAACACA CCATGCTGTC
    ACCTCACAGC TACCACAGGT CCCAGCTGGA GCGGGGAGCC GACCTATTGG GGAGCAGGAA
    GAGGAAGAGT ACGAAACCAA GGGGGGCCGC CGGAGAACAT GGGACGATGA TTATGTGCTC
    AAGCGGCAGT TTTCAGCTTT GGTTCCTGCT TTTGATCCTA GACCTGGTCG AACTAATGTC
    CAGCAGACAA CTGATCTAGA AATTCCACCC CCAGGAACAC CTCATTCAGA GCTTTTGGAA
    GAAGTTGAAT GTACTCCATC ACCTCGATTA GCCCTCACTT TAAAAGTAAC AGGTCTTGGA
    ACAACTCGTG AAGTTGAATT ACCACTCACT AATTTCAGAT CAACCATCTT TTACTATGTA
    CAGAAATTGC TTCAGTTGTC ATGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCAA CATACACAAT TATGTACAGA GAAATGAAGG ATTCTGACAA AGAAAAGGAA
    AATGGAAAAA TGGATTTTTG TGAGCATGGA ACAAAGTCTG GGTTAAACCA GGGGGCCATT
    TCTACTCTTC AAAGTAGTGA TATTCTGAAT TTGACAAAAG AACAACCTCA GGCCAAAGCA
    GGCAATGGGC AGAATTCATG TGGCGTGGAA GACGTCCTTC AGCTCCTGCG TATTCTGTAT
    ATAGTTGCAA GTGACCCTTA CTCAAGAATA TCCCAGGAAG AAGGTGATGA GCAGCCTCAG
    TTTACTTTTC CACCAGATGA ATTCACTAGC AAAAAAATCA CAACAAAAAT TTTGCAGCAA
    ATTGAGGAAC CATTGGCATT GGCAAGTGGA GCTCTGCCAG ACTGGTGTGA ACAGTTAACC
    AGCAAGTGTC CTTTTCTAAT ACCGTTCGAA ACTAGACAGC TTTATTTCAC ATGTACAGCA
    TTTGGTGCAT CAAGAGCAAT TGTGTGGCTA CAAAACCGAC GTGAGGCCAC TGTGGAAAGA
    ACAAGGACCA CAAGTAGTGT GAGGCGAGAT GATCCTGGAG AGTTCCGTGT TGGTCGTCTC
    AAGCACGAAA GAGTAAAAGT TCCACGTGGT GAATCACTGA TGGAGTGGGC CGAAAACGTC
    ATGCAAATAC ACGCAGATCG GAAATCAGTG CTTGAGGTTG AATTTTTAGG AGAAGAAGGA
    ACTGGTCTGG GGCCCACATT AGAGTTCTAT GCTCTGGTAG CAGCAGAATT CCAGAGAACT
    GACTTGGGTG CTTGGCTTTG TGATGACAAC TTTCCAGATG ATGAATCTCG TCATGTCGAT
    CTTGGAGGTG GATTGAAACC TCCCGGATAC TACGTGCAGA GATCATGTGG ACTGTTCACA
    GCTCCATTTC CACAAGACAG TGACGAGCTT GAAAGGATCA CAAAACTCTT TCATTTCCTT
    GGAATTTTCT TGGCCAAATG CATTCAAGAC AATAGACTTG TGGACTTACC TATTTCCAAA
    CCTTTCTTTA AACTTATGTG TATGGGGGAC ATTAAAAGTA ATATGAGCAA ACTGATTTAT
    GAGTCACGAG GCGACAGAGA CCTACACTGC ACTGAAAGTC AGTCTGAAGC CTCTACAGAA
    GAAGGGCATG ACTCACTCTC AGTAGGAAGC TTTGAGGAGG ATTCAAAATC AGAATTCATT
    CTAGATCCCC CCAAACCAAA ACCGCCAGCT TGGTTTAATG GAATTTTAAC TTGGGAAGAC
    TTTGAACTAG TAAACCCACA CAGAGCCAGA TTTTTAAAAG AAATTAAAGA CCTTGCCATC
    AAGAGGCGCC AGATTTTAAG CAACAAAGGT CTTTCTGAAG ATGAGAAGAA CACAAAATTA
    CAAGAACTAG TGCTGAAGAA TCCATCAGGT TCTGGGCCTC CACTTAGCAT AGAGGATTTA
    GGTTTAAATT TCCAGTTTTG CCCTTCCTCA AGAATATATG GTTTTACAGC TGTGGATCTC
    AAGCCAAGTG GTGAAGATGA GATGATAACA ATGGATAATG CAGAAGAATA TGTGGATTTG
    ATGTTTGACT TTTGCATGCA TACGGGTATT CAGAAACAGA TGGAAGCCTT CAGAGATGGG
    TTTAATAAAG TTTTTCCAAT GGAGAAATTA AGTTCCTTCA GCCATGAAGA GGTCCAAATG
    ATTCTTTGTG GAAATCAGTC GCCATCCTGG GCAGCAGAGG ATATTATTAA TTATACTGAA
    CCTAAGTTGG GTTATACACG TGACAGCCCT GGCTTCCTTA GGTTCGTGCG GGTTTTATGT
    GGTATGTCTT CAGACGAGAG GAAAGCATTT CTGCAGTTTA CCACTGGTTG TTCAACTCTG
    CCTCCAGGGG GACTGGCTAA CTTGCATCCC AGGCTCACAG TTGTACGCAA GGTTGATGCT
    ACTGATGCAA GCTACCCATC AGTGAACACA TGTGTGCATT ATCTAAAGTT GCCAGAATAC
    TCTTCGGAGG AGATCATGAG AGAGCGTCTG CTAGCTGCTA CAATGGAGAA AGGCTTTCAT
    CTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu13699
    Clone ID Related Accession (Same CDS sequence) XM_006056608.1
    Accession Version XM_006056608.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784766.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCCG GTACTACATC GACAGTGACT ATGTCAACAT CCAGTGTTAC TAGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAGT CTTTAAGTAA TACTTTAACC
    ACCAGCCTCA CATCAACCTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTGTTAG CTGAGCTAGA TGACGATGAG
    GACTTGCCAG AGCCAGACGA GGAAGATGAT GAAAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGT CCGTCCCTAC AGCGCCGAGC TGGCTCCCGC
    TCTGATGTAA CACACCATGC TGTCACCTCA CAGCTACCAC AGGTCCCAGC TGGAGCGGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAGGAA GAGTACGAAA CCAAGGGGGG CCGCCGGAGA
    ACATGGGACG ATGATTATGT GCTCAAGCGG CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGAACTAA TGTCCAGCAG ACAACTGATC TAGAAATTCC ACCCCCAGGA
    ACACCTCATT CAGAGCTTTT GGAAGAAGTT GAATGTACTC CATCACCTCG ATTAGCCCTC
    ACTTTAAAAG TAACAGGTCT TGGAACAACT CGTGAAGTTG AATTACCACT CACTAATTTC
    AGATCAACCA TCTTTTACTA TGTACAGAAA TTGCTTCAGT TGTCATGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCAACATACA CAATTATGTA CAGAGAAATG
    AAGGATTCTG ACAAAGAAAA GGAAAATGGA AAAATGGGTT GTTGGTCTAT AGAGCATGTG
    GAACAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG
    AATGCAGACG CCGCTTTCCT GCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG
    AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA
    AAGTCTGGGT TAAACCAGGG GGCCATTTCT ACTCTTCAAA GTAGTGATAT TCTGAATTTG
    ACAAAAGAAC AACCTCAGGC CAAAGCAGGC AATGGGCAGA ATTCATGTGG CGTGGAAGAC
    GTCCTTCAGC TCCTGCGTAT TCTGTATATA GTTGCAAGTG ACCCTTACTC AAGAATATCC
    CAGGAAGAAG GTGATGAGCA GCCTCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA
    AAAATCACAA CAAAAATTTT GCAGCAAATT GAGGAACCAT TGGCATTGGC AAGTGGAGCT
    CTGCCAGACT GGTGTGAACA GTTAACCAGC AAGTGTCCTT TTCTAATACC GTTCGAAACT
    AGACAGCTTT ATTTCACATG TACAGCATTT GGTGCATCAA GAGCAATTGT GTGGCTACAA
    AACCGACGTG AGGCCACTGT GGAAAGAACA AGGACCACAA GTAGTGTGAG GCGAGATGAT
    CCTGGAGAGT TCCGTGTTGG TCGTCTCAAG CACGAAAGAG TAAAAGTTCC ACGTGGTGAA
    TCACTGATGG AGTGGGCCGA AAACGTCATG CAAATACACG CAGATCGGAA ATCAGTGCTT
    GAGGTTGAAT TTTTAGGAGA AGAAGGAACT GGTCTGGGGC CCACATTAGA GTTCTATGCT
    CTGGTAGCAG CAGAATTCCA GAGAACTGAC TTGGGTGCTT GGCTTTGTGA TGACAACTTT
    CCAGATGATG AATCTCGTCA TGTCGATCTT GGAGGTGGAT TGAAACCTCC CGGATACTAC
    GTGCAGAGAT CATGTGGACT GTTCACAGCT CCATTTCCAC AAGACAGTGA CGAGCTTGAA
    AGGATCACAA AACTCTTTCA TTTCCTTGGA ATTTTCTTGG CCAAATGCAT TCAAGACAAT
    AGACTTGTGG ACTTACCTAT TTCCAAACCT TTCTTTAAAC TTATGTGTAT GGGGGACATT
    AAAAGTAATA TGAGCAAACT GATTTATGAG TCACGAGGCG ACAGAGACCT ACACTGCACT
    GAAAGTCAGT CTGAAGCCTC TACAGAAGAA GGGCATGACT CACTCTCAGT AGGAAGCTTT
    GAGGAGGATT CAAAATCAGA ATTCATTCTA GATCCCCCCA AACCAAAACC GCCAGCTTGG
    TTTAATGGAA TTTTAACTTG GGAAGACTTT GAACTAGTAA ACCCACACAG AGCCAGATTT
    TTAAAAGAAA TTAAAGACCT TGCCATCAAG AGGCGCCAGA TTTTAAGCAA CAAAGGTCTT
    TCTGAAGATG AGAAGAACAC AAAATTACAA GAACTAGTGC TGAAGAATCC ATCAGGTTCT
    GGGCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA
    ATATATGGTT TTACAGCTGT GGATCTCAAG CCAAGTGGTG AAGATGAGAT GATAACAATG
    GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GCATGCATAC GGGTATTCAG
    AAACAGATGG AAGCCTTCAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT
    TCCTTCAGCC ATGAAGAGGT CCAAATGATT CTTTGTGGAA ATCAGTCGCC ATCCTGGGCA
    GCAGAGGATA TTATTAATTA TACTGAACCT AAGTTGGGTT ATACACGTGA CAGCCCTGGC
    TTCCTTAGGT TCGTGCGGGT TTTATGTGGT ATGTCTTCAG ACGAGAGGAA AGCATTTCTG
    CAGTTTACCA CTGGTTGTTC AACTCTGCCT CCAGGGGGAC TGGCTAACTT GCATCCCAGG
    CTCACAGTTG TACGCAAGGT TGATGCTACT GATGCAAGCT ACCCATCAGT GAACACATGT
    GTGCATTATC TAAAGTTGCC AGAATACTCT TCGGAGGAGA TCATGAGAGA GCGTCTGCTA
    GCTGCTACAA TGGAGAAAGG CTTTCATCTC AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006056670.1
    CDS357..7352
    Translation

    Target ORF information:

    RefSeq Version XM_006056608.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006056608.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCCG GTACTACATC GACAGTGACT ATGTCAACAT CCAGTGTTAC TAGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAGT CTTTAAGTAA TACTTTAACC
    ACCAGCCTCA CATCAACCTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTGTTAG CTGAGCTAGA TGACGATGAG
    GACTTGCCAG AGCCAGACGA GGAAGATGAT GAAAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGT CCGTCCCTAC AGCGCCGAGC TGGCTCCCGC
    TCTGATGTAA CACACCATGC TGTCACCTCA CAGCTACCAC AGGTCCCAGC TGGAGCGGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAGGAA GAGTACGAAA CCAAGGGGGG CCGCCGGAGA
    ACATGGGACG ATGATTATGT GCTCAAGCGG CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGAACTAA TGTCCAGCAG ACAACTGATC TAGAAATTCC ACCCCCAGGA
    ACACCTCATT CAGAGCTTTT GGAAGAAGTT GAATGTACTC CATCACCTCG ATTAGCCCTC
    ACTTTAAAAG TAACAGGTCT TGGAACAACT CGTGAAGTTG AATTACCACT CACTAATTTC
    AGATCAACCA TCTTTTACTA TGTACAGAAA TTGCTTCAGT TGTCATGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCAACATACA CAATTATGTA CAGAGAAATG
    AAGGATTCTG ACAAAGAAAA GGAAAATGGA AAAATGGGTT GTTGGTCTAT AGAGCATGTG
    GAACAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG
    AATGCAGACG CCGCTTTCCT GCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG
    AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA
    AAGTCTGGGT TAAACCAGGG GGCCATTTCT ACTCTTCAAA GTAGTGATAT TCTGAATTTG
    ACAAAAGAAC AACCTCAGGC CAAAGCAGGC AATGGGCAGA ATTCATGTGG CGTGGAAGAC
    GTCCTTCAGC TCCTGCGTAT TCTGTATATA GTTGCAAGTG ACCCTTACTC AAGAATATCC
    CAGGAAGAAG GTGATGAGCA GCCTCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA
    AAAATCACAA CAAAAATTTT GCAGCAAATT GAGGAACCAT TGGCATTGGC AAGTGGAGCT
    CTGCCAGACT GGTGTGAACA GTTAACCAGC AAGTGTCCTT TTCTAATACC GTTCGAAACT
    AGACAGCTTT ATTTCACATG TACAGCATTT GGTGCATCAA GAGCAATTGT GTGGCTACAA
    AACCGACGTG AGGCCACTGT GGAAAGAACA AGGACCACAA GTAGTGTGAG GCGAGATGAT
    CCTGGAGAGT TCCGTGTTGG TCGTCTCAAG CACGAAAGAG TAAAAGTTCC ACGTGGTGAA
    TCACTGATGG AGTGGGCCGA AAACGTCATG CAAATACACG CAGATCGGAA ATCAGTGCTT
    GAGGTTGAAT TTTTAGGAGA AGAAGGAACT GGTCTGGGGC CCACATTAGA GTTCTATGCT
    CTGGTAGCAG CAGAATTCCA GAGAACTGAC TTGGGTGCTT GGCTTTGTGA TGACAACTTT
    CCAGATGATG AATCTCGTCA TGTCGATCTT GGAGGTGGAT TGAAACCTCC CGGATACTAC
    GTGCAGAGAT CATGTGGACT GTTCACAGCT CCATTTCCAC AAGACAGTGA CGAGCTTGAA
    AGGATCACAA AACTCTTTCA TTTCCTTGGA ATTTTCTTGG CCAAATGCAT TCAAGACAAT
    AGACTTGTGG ACTTACCTAT TTCCAAACCT TTCTTTAAAC TTATGTGTAT GGGGGACATT
    AAAAGTAATA TGAGCAAACT GATTTATGAG TCACGAGGCG ACAGAGACCT ACACTGCACT
    GAAAGTCAGT CTGAAGCCTC TACAGAAGAA GGGCATGACT CACTCTCAGT AGGAAGCTTT
    GAGGAGGATT CAAAATCAGA ATTCATTCTA GATCCCCCCA AACCAAAACC GCCAGCTTGG
    TTTAATGGAA TTTTAACTTG GGAAGACTTT GAACTAGTAA ACCCACACAG AGCCAGATTT
    TTAAAAGAAA TTAAAGACCT TGCCATCAAG AGGCGCCAGA TTTTAAGCAA CAAAGGTCTT
    TCTGAAGATG AGAAGAACAC AAAATTACAA GAACTAGTGC TGAAGAATCC ATCAGGTTCT
    GGGCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA
    ATATATGGTT TTACAGCTGT GGATCTCAAG CCAAGTGGTG AAGATGAGAT GATAACAATG
    GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GCATGCATAC GGGTATTCAG
    AAACAGATGG AAGCCTTCAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT
    TCCTTCAGCC ATGAAGAGGT CCAAATGATT CTTTGTGGAA ATCAGTCGCC ATCCTGGGCA
    GCAGAGGATA TTATTAATTA TACTGAACCT AAGTTGGGTT ATACACGTGA CAGCCCTGGC
    TTCCTTAGGT TCGTGCGGGT TTTATGTGGT ATGTCTTCAG ACGAGAGGAA AGCATTTCTG
    CAGTTTACCA CTGGTTGTTC AACTCTGCCT CCAGGGGGAC TGGCTAACTT GCATCCCAGG
    CTCACAGTTG TACGCAAGGT TGATGCTACT GATGCAAGCT ACCCATCAGT GAACACATGT
    GTGCATTATC TAAAGTTGCC AGAATACTCT TCGGAGGAGA TCATGAGAGA GCGTCTGCTA
    GCTGCTACAA TGGAGAAAGG CTTTCATCTC AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu56860
    Clone ID Related Accession (Same CDS sequence) XM_025271334.1 , XM_006056604.2
    Accession Version XM_025271334.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CACAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCAGG AGCGGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACCA AGGGGGGCCG CCGGAGAACA
    TGGGACGATG ATTATGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCATTGG CATTGGCAAG TGGAGCTCTG
    CCAGATTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAAAA CGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT CTGGGGCCCA CATTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGGGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTACGTG
    CAGAGATCAT GTGGACTGTT CACAGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGATCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGACGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCGCCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG CCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTAA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025127119.1
    CDS484..8316
    Translation

    Target ORF information:

    RefSeq Version XM_025271334.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025271334.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CACAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCAGG AGCGGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACCA AGGGGGGCCG CCGGAGAACA
    TGGGACGATG ATTATGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCATTGG CATTGGCAAG TGGAGCTCTG
    CCAGATTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAAAA CGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT CTGGGGCCCA CATTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGGGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTACGTG
    CAGAGATCAT GTGGACTGTT CACAGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGATCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGACGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCGCCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG CCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTAA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu56860
    Clone ID Related Accession (Same CDS sequence) XM_025271334.1 , XM_006056604.2
    Accession Version XM_006056604.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006056604.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CACAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCAGG AGCGGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACCA AGGGGGGCCG CCGGAGAACA
    TGGGACGATG ATTATGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCATTGG CATTGGCAAG TGGAGCTCTG
    CCAGATTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAAAA CGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT CTGGGGCCCA CATTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGGGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTACGTG
    CAGAGATCAT GTGGACTGTT CACAGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGATCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGACGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCGCCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG CCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTAA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006056666.1
    CDS368..8200
    Translation

    Target ORF information:

    RefSeq Version XM_006056604.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006056604.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CACAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCAGG AGCGGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACCA AGGGGGGCCG CCGGAGAACA
    TGGGACGATG ATTATGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGGTTGTT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGACGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT GAATTTGACA
    AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGGCAGAATT CATGTGGCGT GGAAGACGTC
    CTTCAGCTCC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTACTCAAG AATATCCCAG
    GAAGAAGGTG ATGAGCAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA
    ATCACAACAA AAATTTTGCA GCAAATTGAG GAACCATTGG CATTGGCAAG TGGAGCTCTG
    CCAGATTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTTC TAATACCGTT CGAAACTAGA
    CAGCTTTATT TCACATGTAC AGCATTTGGT GCATCAAGAG CAATTGTGTG GCTACAAAAC
    CGACGTGAGG CCACTGTGGA AAGAACAAGG ACCACAAGTA GTGTGAGGCG AGATGATCCT
    GGAGAGTTCC GTGTTGGTCG TCTCAAGCAC GAAAGAGTAA AAGTTCCACG TGGTGAATCA
    CTGATGGAGT GGGCCGAAAA CGTCATGCAA ATACACGCAG ATCGGAAATC AGTGCTTGAG
    GTTGAATTTT TAGGAGAAGA AGGAACTGGT CTGGGGCCCA CATTAGAGTT CTATGCTCTG
    GTAGCAGCAG AATTCCAGAG AACTGACTTG GGGGCTTGGC TTTGTGATGA CAACTTTCCA
    GATGATGAAT CTCGTCATGT CGATCTTGGA GGTGGATTGA AACCTCCCGG ATACTACGTG
    CAGAGATCAT GTGGACTGTT CACAGCTCCA TTTCCACAAG ACAGTGACGA GCTTGAAAGG
    ATCACAAAAC TCTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA
    CTTGTGGACT TACCTATTTC CAAACCTTTC TTTAAACTTA TGTGTATGGG GGACATTAAA
    AGTAATATGA GCAAACTGAT TTATGAGTCA CGAGGCGACA GAGACCTACA CTGCACTGAA
    AGTCAGTCTG AAGCCTCTAC AGAAGAAGGG CATGACTCAC TCTCAGTAGG AAGCTTTGAG
    GAGGATTCAA AATCAGAATT CATTCTAGAT CCCCCCAAAC CAAAACCGCC AGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGACTTTGAA CTAGTAAACC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGATCTTGC CATCAAGAGG CGCCAGATTT TAAGCAACAA AGGTCTTTCT
    GAAGACGAGA AGAACACAAA ATTACAAGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG
    CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA
    TATGGTTTTA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGCA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CCTTCAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AAGAGGTCCA AATGATTCTT TGTGGAAATC AGTCGCCATC CTGGGCAGCA
    GAGGATATTA TTAATTATAC TGAACCTAAG TTGGGTTATA CACGTGACAG CCCTGGCTTC
    CTTAGGTTCG TGCGGGTTTT ATGTGGTATG TCTTCAGACG AGAGGAAAGC ATTTCTGCAG
    TTTACCACTG GTTGTTCAAC TCTGCCTCCA GGGGGACTGG CTAACTTGCA TCCCAGGCTC
    ACAGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTACC CATCAGTGAA CACATGTGTG
    CATTATCTAA AGTTGCCAGA ATACTCTTCG GAGGAGATCA TGAGAGAGCG TCTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu56861
    Clone ID Related Accession (Same CDS sequence) XM_025271335.1
    Accession Version XM_025271335.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CACAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCGTCCCTA CAGCGCCGAG CTGGCTCCCG CTCTGATGTA
    ACACACCATG CTGTCACCTC ACAGCTACCA CAGGTCCCAG CAGGAGCGGG GAGCCGACCT
    ATTGGGGAGC AGGAAGAGGA AGAGTACGAA ACCAAGGGGG GCCGCCGGAG AACATGGGAC
    GATGATTATG TGCTCAAGCG GCAGTTTTCA GCTTTGGTTC CTGCTTTTGA TCCTAGACCT
    GGTCGAACTA ATGTCCAGCA GACAACTGAT CTAGAAATTC CACCCCCAGG AACACCTCAT
    TCAGAGCTTT TGGAAGAAGT TGAATGTACT CCATCACCTC GATTAGCCCT CACTTTAAAA
    GTAACAGGTC TTGGAACAAC TCGTGAAGTT GAATTACCAC TCACTAATTT CAGATCAACC
    ATCTTTTACT ATGTACAGAA ATTGCTTCAG TTGTCATGTA ATGGCAATGT GAAATCAGAT
    AAACTTAGGC GTATTTGGGA GCCAACATAC ACAATTATGT ACAGAGAAAT GAAGGATTCT
    GACAAAGAAA AGGAAAATGG AAAAATGGGT TGTTGGTCTA TAGAGCATGT GGAACAGTAC
    CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAC
    GCCGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTCATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGG
    TTAAACCAGG GGGCCATTTC TACTCTTCAA AGTAGTGATA TTCTGAATTT GACAAAAGAA
    CAACCTCAGG CCAAAGCAGG CAATGGGCAG AATTCATGTG GCGTGGAAGA CGTCCTTCAG
    CTCCTGCGTA TTCTGTATAT AGTTGCAAGT GACCCTTACT CAAGAATATC CCAGGAAGAA
    GGTGATGAGC AGCCTCAGTT TACTTTTCCA CCAGATGAAT TCACTAGCAA AAAAATCACA
    ACAAAAATTT TGCAGCAAAT TGAGGAACCA TTGGCATTGG CAAGTGGAGC TCTGCCAGAT
    TGGTGTGAAC AGTTAACCAG CAAGTGTCCT TTTCTAATAC CGTTCGAAAC TAGACAGCTT
    TATTTCACAT GTACAGCATT TGGTGCATCA AGAGCAATTG TGTGGCTACA AAACCGACGT
    GAGGCCACTG TGGAAAGAAC AAGGACCACA AGTAGTGTGA GGCGAGATGA TCCTGGAGAG
    TTCCGTGTTG GTCGTCTCAA GCACGAAAGA GTAAAAGTTC CACGTGGTGA ATCACTGATG
    GAGTGGGCCG AAAACGTCAT GCAAATACAC GCAGATCGGA AATCAGTGCT TGAGGTTGAA
    TTTTTAGGAG AAGAAGGAAC TGGTCTGGGG CCCACATTAG AGTTCTATGC TCTGGTAGCA
    GCAGAATTCC AGAGAACTGA CTTGGGGGCT TGGCTTTGTG ATGACAACTT TCCAGATGAT
    GAATCTCGTC ATGTCGATCT TGGAGGTGGA TTGAAACCTC CCGGATACTA CGTGCAGAGA
    TCATGTGGAC TGTTCACAGC TCCATTTCCA CAAGACAGTG ACGAGCTTGA AAGGATCACA
    AAACTCTTTC ATTTCCTTGG AATTTTCTTG GCCAAATGCA TTCAAGACAA TAGACTTGTG
    GACTTACCTA TTTCCAAACC TTTCTTTAAA CTTATGTGTA TGGGGGACAT TAAAAGTAAT
    ATGAGCAAAC TGATTTATGA GTCACGAGGC GACAGAGACC TACACTGCAC TGAAAGTCAG
    TCTGAAGCCT CTACAGAAGA AGGGCATGAC TCACTCTCAG TAGGAAGCTT TGAGGAGGAT
    TCAAAATCAG AATTCATTCT AGATCCCCCC AAACCAAAAC CGCCAGCTTG GTTTAATGGA
    ATTTTAACTT GGGAAGACTT TGAACTAGTA AACCCACACA GAGCCAGATT TTTAAAAGAA
    ATTAAAGATC TTGCCATCAA GAGGCGCCAG ATTTTAAGCA ACAAAGGTCT TTCTGAAGAC
    GAGAAGAACA CAAAATTACA AGAACTAGTG CTGAAGAATC CATCAGGTTC TGGGCCTCCA
    CTTAGCATAG AGGATTTAGG TTTAAATTTC CAGTTTTGCC CTTCCTCAAG AATATATGGT
    TTTACAGCTG TGGATCTCAA GCCAAGTGGT GAAGATGAGA TGATAACAAT GGATAATGCA
    GAAGAATATG TGGATTTGAT GTTTGACTTT TGCATGCATA CGGGTATTCA GAAACAGATG
    GAAGCCTTCA GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC
    CATGAAGAGG TCCAAATGAT TCTTTGTGGA AATCAGTCGC CATCCTGGGC AGCAGAGGAT
    ATTATTAATT ATACTGAACC TAAGTTGGGT TATACACGTG ACAGCCCTGG CTTCCTTAGG
    TTCGTGCGGG TTTTATGTGG TATGTCTTCA GACGAGAGGA AAGCATTTCT GCAGTTTACC
    ACTGGTTGTT CAACTCTGCC TCCAGGGGGA CTGGCTAACT TGCATCCCAG GCTCACAGTT
    GTACGCAAGG TTGATGCTAC TGATGCAAGC TACCCATCAG TGAACACATG TGTGCATTAT
    CTAAAGTTGC CAGAATACTC TTCGGAGGAG ATCATGAGAG AGCGTCTGCT AGCTGCTACA
    ATGGAGAAAG GCTTTCATCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025127120.1
    CDS484..8310
    Translation

    Target ORF information:

    RefSeq Version XM_025271335.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025271335.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CACAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCGTCCCTA CAGCGCCGAG CTGGCTCCCG CTCTGATGTA
    ACACACCATG CTGTCACCTC ACAGCTACCA CAGGTCCCAG CAGGAGCGGG GAGCCGACCT
    ATTGGGGAGC AGGAAGAGGA AGAGTACGAA ACCAAGGGGG GCCGCCGGAG AACATGGGAC
    GATGATTATG TGCTCAAGCG GCAGTTTTCA GCTTTGGTTC CTGCTTTTGA TCCTAGACCT
    GGTCGAACTA ATGTCCAGCA GACAACTGAT CTAGAAATTC CACCCCCAGG AACACCTCAT
    TCAGAGCTTT TGGAAGAAGT TGAATGTACT CCATCACCTC GATTAGCCCT CACTTTAAAA
    GTAACAGGTC TTGGAACAAC TCGTGAAGTT GAATTACCAC TCACTAATTT CAGATCAACC
    ATCTTTTACT ATGTACAGAA ATTGCTTCAG TTGTCATGTA ATGGCAATGT GAAATCAGAT
    AAACTTAGGC GTATTTGGGA GCCAACATAC ACAATTATGT ACAGAGAAAT GAAGGATTCT
    GACAAAGAAA AGGAAAATGG AAAAATGGGT TGTTGGTCTA TAGAGCATGT GGAACAGTAC
    CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAC
    GCCGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTCATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGG
    TTAAACCAGG GGGCCATTTC TACTCTTCAA AGTAGTGATA TTCTGAATTT GACAAAAGAA
    CAACCTCAGG CCAAAGCAGG CAATGGGCAG AATTCATGTG GCGTGGAAGA CGTCCTTCAG
    CTCCTGCGTA TTCTGTATAT AGTTGCAAGT GACCCTTACT CAAGAATATC CCAGGAAGAA
    GGTGATGAGC AGCCTCAGTT TACTTTTCCA CCAGATGAAT TCACTAGCAA AAAAATCACA
    ACAAAAATTT TGCAGCAAAT TGAGGAACCA TTGGCATTGG CAAGTGGAGC TCTGCCAGAT
    TGGTGTGAAC AGTTAACCAG CAAGTGTCCT TTTCTAATAC CGTTCGAAAC TAGACAGCTT
    TATTTCACAT GTACAGCATT TGGTGCATCA AGAGCAATTG TGTGGCTACA AAACCGACGT
    GAGGCCACTG TGGAAAGAAC AAGGACCACA AGTAGTGTGA GGCGAGATGA TCCTGGAGAG
    TTCCGTGTTG GTCGTCTCAA GCACGAAAGA GTAAAAGTTC CACGTGGTGA ATCACTGATG
    GAGTGGGCCG AAAACGTCAT GCAAATACAC GCAGATCGGA AATCAGTGCT TGAGGTTGAA
    TTTTTAGGAG AAGAAGGAAC TGGTCTGGGG CCCACATTAG AGTTCTATGC TCTGGTAGCA
    GCAGAATTCC AGAGAACTGA CTTGGGGGCT TGGCTTTGTG ATGACAACTT TCCAGATGAT
    GAATCTCGTC ATGTCGATCT TGGAGGTGGA TTGAAACCTC CCGGATACTA CGTGCAGAGA
    TCATGTGGAC TGTTCACAGC TCCATTTCCA CAAGACAGTG ACGAGCTTGA AAGGATCACA
    AAACTCTTTC ATTTCCTTGG AATTTTCTTG GCCAAATGCA TTCAAGACAA TAGACTTGTG
    GACTTACCTA TTTCCAAACC TTTCTTTAAA CTTATGTGTA TGGGGGACAT TAAAAGTAAT
    ATGAGCAAAC TGATTTATGA GTCACGAGGC GACAGAGACC TACACTGCAC TGAAAGTCAG
    TCTGAAGCCT CTACAGAAGA AGGGCATGAC TCACTCTCAG TAGGAAGCTT TGAGGAGGAT
    TCAAAATCAG AATTCATTCT AGATCCCCCC AAACCAAAAC CGCCAGCTTG GTTTAATGGA
    ATTTTAACTT GGGAAGACTT TGAACTAGTA AACCCACACA GAGCCAGATT TTTAAAAGAA
    ATTAAAGATC TTGCCATCAA GAGGCGCCAG ATTTTAAGCA ACAAAGGTCT TTCTGAAGAC
    GAGAAGAACA CAAAATTACA AGAACTAGTG CTGAAGAATC CATCAGGTTC TGGGCCTCCA
    CTTAGCATAG AGGATTTAGG TTTAAATTTC CAGTTTTGCC CTTCCTCAAG AATATATGGT
    TTTACAGCTG TGGATCTCAA GCCAAGTGGT GAAGATGAGA TGATAACAAT GGATAATGCA
    GAAGAATATG TGGATTTGAT GTTTGACTTT TGCATGCATA CGGGTATTCA GAAACAGATG
    GAAGCCTTCA GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC
    CATGAAGAGG TCCAAATGAT TCTTTGTGGA AATCAGTCGC CATCCTGGGC AGCAGAGGAT
    ATTATTAATT ATACTGAACC TAAGTTGGGT TATACACGTG ACAGCCCTGG CTTCCTTAGG
    TTCGTGCGGG TTTTATGTGG TATGTCTTCA GACGAGAGGA AAGCATTTCT GCAGTTTACC
    ACTGGTTGTT CAACTCTGCC TCCAGGGGGA CTGGCTAACT TGCATCCCAG GCTCACAGTT
    GTACGCAAGG TTGATGCTAC TGATGCAAGC TACCCATCAG TGAACACATG TGTGCATTAT
    CTAAAGTTGC CAGAATACTC TTCGGAGGAG ATCATGAGAG AGCGTCTGCT AGCTGCTACA
    ATGGAGAAAG GCTTTCATCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu56862
    Clone ID Related Accession (Same CDS sequence) XM_025271336.1
    Accession Version XM_025271336.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCCCTGAT
    AGTTCTGCTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTA ACTAATAAAG AAGCAGCTTC ACAACGGCCC
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC CGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CCAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCCGGTAC TACATCGACA
    GTGACTATGT CAACATCCAG TGTTACTAGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAGTCTTT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAGCC AGACGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCGT CCCTACAGCG CCGAGCTGGC TCCCGCTCTG ATGTAACACA CCATGCTGTC
    ACCTCACAGC TACCACAGGT CCCAGCAGGA GCGGGGAGCC GACCTATTGG GGAGCAGGAA
    GAGGAAGAGT ACGAAACCAA GGGGGGCCGC CGGAGAACAT GGGACGATGA TTATGTGCTC
    AAGCGGCAGT TTTCAGCTTT GGTTCCTGCT TTTGATCCTA GACCTGGTCG AACTAATGTC
    CAGCAGACAA CTGATCTAGA AATTCCACCC CCAGGAACAC CTCATTCAGA GCTTTTGGAA
    GAAGTTGAAT GTACTCCATC ACCTCGATTA GCCCTCACTT TAAAAGTAAC AGGTCTTGGA
    ACAACTCGTG AAGTTGAATT ACCACTCACT AATTTCAGAT CAACCATCTT TTACTATGTA
    CAGAAATTGC TTCAGTTGTC ATGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCAA CATACACAAT TATGTACAGA GAAATGAAGG ATTCTGACAA AGAAAAGGAA
    AATGGAAAAA TGGGTTGTTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGATGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCCGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC
    ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGGTTAAA CCAGGGGGCC
    ATTTCTACTC TTCAAAGTAG TGATATTCTG AATTTGACAA AAGAACAACC TCAGGCCAAA
    GCAGGCAATG GGCAGAATTC ATGTGGCGTG GAAGACGTCC TTCAGCTCCT GCGTATTCTG
    TATATAGTTG CAAGTGACCC TTACTCAAGA ATATCCCAGG AAGAAGGTGA TGAGCAGCCT
    CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TCACAACAAA AATTTTGCAG
    CAAATTGAGG AACCATTGGC ATTGGCAAGT GGAGCTCTGC CAGATTGGTG TGAACAGTTA
    ACCAGCAAGT GTCCTTTTCT AATACCGTTC GAAACTAGAC AGCTTTATTT CACATGTACA
    GCATTTGGTG CATCAAGAGC AATTGTGTGG CTACAAAACC GACGTGAGGC CACTGTGGAA
    AGAACAAGGA CCACAAGTAG TGTGAGGCGA GATGATCCTG GAGAGTTCCG TGTTGGTCGT
    CTCAAGCACG AAAGAGTAAA AGTTCCACGT GGTGAATCAC TGATGGAGTG GGCCGAAAAC
    GTCATGCAAA TACACGCAGA TCGGAAATCA GTGCTTGAGG TTGAATTTTT AGGAGAAGAA
    GGAACTGGTC TGGGGCCCAC ATTAGAGTTC TATGCTCTGG TAGCAGCAGA ATTCCAGAGA
    ACTGACTTGG GGGCTTGGCT TTGTGATGAC AACTTTCCAG ATGATGAATC TCGTCATGTC
    GATCTTGGAG GTGGATTGAA ACCTCCCGGA TACTACGTGC AGAGATCATG TGGACTGTTC
    ACAGCTCCAT TTCCACAAGA CAGTGACGAG CTTGAAAGGA TCACAAAACT CTTTCATTTC
    CTTGGAATTT TCTTGGCCAA ATGCATTCAA GACAATAGAC TTGTGGACTT ACCTATTTCC
    AAACCTTTCT TTAAACTTAT GTGTATGGGG GACATTAAAA GTAATATGAG CAAACTGATT
    TATGAGTCAC GAGGCGACAG AGACCTACAC TGCACTGAAA GTCAGTCTGA AGCCTCTACA
    GAAGAAGGGC ATGACTCACT CTCAGTAGGA AGCTTTGAGG AGGATTCAAA ATCAGAATTC
    ATTCTAGATC CCCCCAAACC AAAACCGCCA GCTTGGTTTA ATGGAATTTT AACTTGGGAA
    GACTTTGAAC TAGTAAACCC ACACAGAGCC AGATTTTTAA AAGAAATTAA AGATCTTGCC
    ATCAAGAGGC GCCAGATTTT AAGCAACAAA GGTCTTTCTG AAGACGAGAA GAACACAAAA
    TTACAAGAAC TAGTGCTGAA GAATCCATCA GGTTCTGGGC CTCCACTTAG CATAGAGGAT
    TTAGGTTTAA ATTTCCAGTT TTGCCCTTCC TCAAGAATAT ATGGTTTTAC AGCTGTGGAT
    CTCAAGCCAA GTGGTGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTTTGCAT GCATACGGGT ATTCAGAAAC AGATGGAAGC CTTCAGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA AGAGGTCCAA
    ATGATTCTTT GTGGAAATCA GTCGCCATCC TGGGCAGCAG AGGATATTAT TAATTATACT
    GAACCTAAGT TGGGTTATAC ACGTGACAGC CCTGGCTTCC TTAGGTTCGT GCGGGTTTTA
    TGTGGTATGT CTTCAGACGA GAGGAAAGCA TTTCTGCAGT TTACCACTGG TTGTTCAACT
    CTGCCTCCAG GGGGACTGGC TAACTTGCAT CCCAGGCTCA CAGTTGTACG CAAGGTTGAT
    GCTACTGATG CAAGCTACCC ATCAGTGAAC ACATGTGTGC ATTATCTAAA GTTGCCAGAA
    TACTCTTCGG AGGAGATCAT GAGAGAGCGT CTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025127121.1
    CDS484..8175
    Translation

    Target ORF information:

    RefSeq Version XM_025271336.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025271336.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGT
    ACCAGCACCT TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGAAAGTT AGGCCCTGAT
    AGTTCTGCTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT TAGTGTTAGT
    TCTCCTGATG TTAGTTCAGT ATCTGAGTTA ACTAATAAAG AAGCAGCTTC ACAACGGCCC
    CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC CGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CCAACAACAT GAATCTAAGC
    CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCCGGTAC TACATCGACA
    GTGACTATGT CAACATCCAG TGTTACTAGC AGCAGCAATG TAGCTACAGC AACAACAGTT
    TTATCAGTTG GTCAGTCTTT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCCAGT
    GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT
    GCCAGTACTC TGTTAGCTGA GCTAGATGAC GATGAGGACT TGCCAGAGCC AGACGAGGAA
    GATGATGAAA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCGT CCCTACAGCG CCGAGCTGGC TCCCGCTCTG ATGTAACACA CCATGCTGTC
    ACCTCACAGC TACCACAGGT CCCAGCAGGA GCGGGGAGCC GACCTATTGG GGAGCAGGAA
    GAGGAAGAGT ACGAAACCAA GGGGGGCCGC CGGAGAACAT GGGACGATGA TTATGTGCTC
    AAGCGGCAGT TTTCAGCTTT GGTTCCTGCT TTTGATCCTA GACCTGGTCG AACTAATGTC
    CAGCAGACAA CTGATCTAGA AATTCCACCC CCAGGAACAC CTCATTCAGA GCTTTTGGAA
    GAAGTTGAAT GTACTCCATC ACCTCGATTA GCCCTCACTT TAAAAGTAAC AGGTCTTGGA
    ACAACTCGTG AAGTTGAATT ACCACTCACT AATTTCAGAT CAACCATCTT TTACTATGTA
    CAGAAATTGC TTCAGTTGTC ATGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT
    TGGGAGCCAA CATACACAAT TATGTACAGA GAAATGAAGG ATTCTGACAA AGAAAAGGAA
    AATGGAAAAA TGGGTTGTTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGATGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCCGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC
    ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGGTTAAA CCAGGGGGCC
    ATTTCTACTC TTCAAAGTAG TGATATTCTG AATTTGACAA AAGAACAACC TCAGGCCAAA
    GCAGGCAATG GGCAGAATTC ATGTGGCGTG GAAGACGTCC TTCAGCTCCT GCGTATTCTG
    TATATAGTTG CAAGTGACCC TTACTCAAGA ATATCCCAGG AAGAAGGTGA TGAGCAGCCT
    CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TCACAACAAA AATTTTGCAG
    CAAATTGAGG AACCATTGGC ATTGGCAAGT GGAGCTCTGC CAGATTGGTG TGAACAGTTA
    ACCAGCAAGT GTCCTTTTCT AATACCGTTC GAAACTAGAC AGCTTTATTT CACATGTACA
    GCATTTGGTG CATCAAGAGC AATTGTGTGG CTACAAAACC GACGTGAGGC CACTGTGGAA
    AGAACAAGGA CCACAAGTAG TGTGAGGCGA GATGATCCTG GAGAGTTCCG TGTTGGTCGT
    CTCAAGCACG AAAGAGTAAA AGTTCCACGT GGTGAATCAC TGATGGAGTG GGCCGAAAAC
    GTCATGCAAA TACACGCAGA TCGGAAATCA GTGCTTGAGG TTGAATTTTT AGGAGAAGAA
    GGAACTGGTC TGGGGCCCAC ATTAGAGTTC TATGCTCTGG TAGCAGCAGA ATTCCAGAGA
    ACTGACTTGG GGGCTTGGCT TTGTGATGAC AACTTTCCAG ATGATGAATC TCGTCATGTC
    GATCTTGGAG GTGGATTGAA ACCTCCCGGA TACTACGTGC AGAGATCATG TGGACTGTTC
    ACAGCTCCAT TTCCACAAGA CAGTGACGAG CTTGAAAGGA TCACAAAACT CTTTCATTTC
    CTTGGAATTT TCTTGGCCAA ATGCATTCAA GACAATAGAC TTGTGGACTT ACCTATTTCC
    AAACCTTTCT TTAAACTTAT GTGTATGGGG GACATTAAAA GTAATATGAG CAAACTGATT
    TATGAGTCAC GAGGCGACAG AGACCTACAC TGCACTGAAA GTCAGTCTGA AGCCTCTACA
    GAAGAAGGGC ATGACTCACT CTCAGTAGGA AGCTTTGAGG AGGATTCAAA ATCAGAATTC
    ATTCTAGATC CCCCCAAACC AAAACCGCCA GCTTGGTTTA ATGGAATTTT AACTTGGGAA
    GACTTTGAAC TAGTAAACCC ACACAGAGCC AGATTTTTAA AAGAAATTAA AGATCTTGCC
    ATCAAGAGGC GCCAGATTTT AAGCAACAAA GGTCTTTCTG AAGACGAGAA GAACACAAAA
    TTACAAGAAC TAGTGCTGAA GAATCCATCA GGTTCTGGGC CTCCACTTAG CATAGAGGAT
    TTAGGTTTAA ATTTCCAGTT TTGCCCTTCC TCAAGAATAT ATGGTTTTAC AGCTGTGGAT
    CTCAAGCCAA GTGGTGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTTTGCAT GCATACGGGT ATTCAGAAAC AGATGGAAGC CTTCAGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA AGAGGTCCAA
    ATGATTCTTT GTGGAAATCA GTCGCCATCC TGGGCAGCAG AGGATATTAT TAATTATACT
    GAACCTAAGT TGGGTTATAC ACGTGACAGC CCTGGCTTCC TTAGGTTCGT GCGGGTTTTA
    TGTGGTATGT CTTCAGACGA GAGGAAAGCA TTTCTGCAGT TTACCACTGG TTGTTCAACT
    CTGCCTCCAG GGGGACTGGC TAACTTGCAT CCCAGGCTCA CAGTTGTACG CAAGGTTGAT
    GCTACTGATG CAAGCTACCC ATCAGTGAAC ACATGTGTGC ATTATCTAAA GTTGCCAGAA
    TACTCTTCGG AGGAGATCAT GAGAGAGCGT CTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu56863
    Clone ID Related Accession (Same CDS sequence) XM_025271337.1
    Accession Version XM_025271337.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7650bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    7621
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CACAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCAGG AGCGGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACCA AGGGGGGCCG CCGGAGAACA
    TGGGACGATG ATTATGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGATTTTT GTGAGCATGG AACAAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTGAA TTTGACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGG CAGAATTCAT GTGGCGTGGA AGACGTCCTT
    CAGCTCCTGC GTATTCTGTA TATAGTTGCA AGTGACCCTT ACTCAAGAAT ATCCCAGGAA
    GAAGGTGATG AGCAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATC
    ACAACAAAAA TTTTGCAGCA AATTGAGGAA CCATTGGCAT TGGCAAGTGG AGCTCTGCCA
    GATTGGTGTG AACAGTTAAC CAGCAAGTGT CCTTTTCTAA TACCGTTCGA AACTAGACAG
    CTTTATTTCA CATGTACAGC ATTTGGTGCA TCAAGAGCAA TTGTGTGGCT ACAAAACCGA
    CGTGAGGCCA CTGTGGAAAG AACAAGGACC ACAAGTAGTG TGAGGCGAGA TGATCCTGGA
    GAGTTCCGTG TTGGTCGTCT CAAGCACGAA AGAGTAAAAG TTCCACGTGG TGAATCACTG
    ATGGAGTGGG CCGAAAACGT CATGCAAATA CACGCAGATC GGAAATCAGT GCTTGAGGTT
    GAATTTTTAG GAGAAGAAGG AACTGGTCTG GGGCCCACAT TAGAGTTCTA TGCTCTGGTA
    GCAGCAGAAT TCCAGAGAAC TGACTTGGGG GCTTGGCTTT GTGATGACAA CTTTCCAGAT
    GATGAATCTC GTCATGTCGA TCTTGGAGGT GGATTGAAAC CTCCCGGATA CTACGTGCAG
    AGATCATGTG GACTGTTCAC AGCTCCATTT CCACAAGACA GTGACGAGCT TGAAAGGATC
    ACAAAACTCT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT
    GTGGACTTAC CTATTTCCAA ACCTTTCTTT AAACTTATGT GTATGGGGGA CATTAAAAGT
    AATATGAGCA AACTGATTTA TGAGTCACGA GGCGACAGAG ACCTACACTG CACTGAAAGT
    CAGTCTGAAG CCTCTACAGA AGAAGGGCAT GACTCACTCT CAGTAGGAAG CTTTGAGGAG
    GATTCAAAAT CAGAATTCAT TCTAGATCCC CCCAAACCAA AACCGCCAGC TTGGTTTAAT
    GGAATTTTAA CTTGGGAAGA CTTTGAACTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ATCTTGCCAT CAAGAGGCGC CAGATTTTAA GCAACAAAGG TCTTTCTGAA
    GACGAGAAGA ACACAAAATT ACAAGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT
    CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT
    GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGCATGC ATACGGGTAT TCAGAAACAG
    ATGGAAGCCT TCAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAAG AGGTCCAAAT GATTCTTTGT GGAAATCAGT CGCCATCCTG GGCAGCAGAG
    GATATTATTA ATTATACTGA ACCTAAGTTG GGTTATACAC GTGACAGCCC TGGCTTCCTT
    AGGTTCGTGC GGGTTTTATG TGGTATGTCT TCAGACGAGA GGAAAGCATT TCTGCAGTTT
    ACCACTGGTT GTTCAACTCT GCCTCCAGGG GGACTGGCTA ACTTGCATCC CAGGCTCACA
    GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTACCCAT CAGTGAACAC ATGTGTGCAT
    TATCTAAAGT TGCCAGAATA CTCTTCGGAG GAGATCATGA GAGAGCGTCT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025127122.1
    CDS484..8133
    Translation

    Target ORF information:

    RefSeq Version XM_025271337.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025271337.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG TACCAGCACC TTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGAAAGT TAGGCCCTGA TAGTTCTGCT CGTACTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAGTT AACTAATAAA
    GAAGCAGCTT CACAACGGCC CCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT
    TTGTTGGCTG CCGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG
    AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCCGGTA CTACATCGAC AGTGACTATG TCAACATCCA GTGTTACTAG CAGCAGCAAT
    GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCTT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT
    GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTAGCTG AGCTAGATGA CGATGAGGAC
    TTGCCAGAGC CAGACGAGGA AGATGATGAA AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCG TCCCTACAGC GCCGAGCTGG CTCCCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCAGCAGG AGCGGGGAGC
    CGACCTATTG GGGAGCAGGA AGAGGAAGAG TACGAAACCA AGGGGGGCCG CCGGAGAACA
    TGGGACGATG ATTATGTGCT CAAGCGGCAG TTTTCAGCTT TGGTTCCTGC TTTTGATCCT
    AGACCTGGTC GAACTAATGT CCAGCAGACA ACTGATCTAG AAATTCCACC CCCAGGAACA
    CCTCATTCAG AGCTTTTGGA AGAAGTTGAA TGTACTCCAT CACCTCGATT AGCCCTCACT
    TTAAAAGTAA CAGGTCTTGG AACAACTCGT GAAGTTGAAT TACCACTCAC TAATTTCAGA
    TCAACCATCT TTTACTATGT ACAGAAATTG CTTCAGTTGT CATGTAATGG CAATGTGAAA
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCA ACATACACAA TTATGTACAG AGAAATGAAG
    GATTCTGACA AAGAAAAGGA AAATGGAAAA ATGGATTTTT GTGAGCATGG AACAAAGTCT
    GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTGAA TTTGACAAAA
    GAACAACCTC AGGCCAAAGC AGGCAATGGG CAGAATTCAT GTGGCGTGGA AGACGTCCTT
    CAGCTCCTGC GTATTCTGTA TATAGTTGCA AGTGACCCTT ACTCAAGAAT ATCCCAGGAA
    GAAGGTGATG AGCAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATC
    ACAACAAAAA TTTTGCAGCA AATTGAGGAA CCATTGGCAT TGGCAAGTGG AGCTCTGCCA
    GATTGGTGTG AACAGTTAAC CAGCAAGTGT CCTTTTCTAA TACCGTTCGA AACTAGACAG
    CTTTATTTCA CATGTACAGC ATTTGGTGCA TCAAGAGCAA TTGTGTGGCT ACAAAACCGA
    CGTGAGGCCA CTGTGGAAAG AACAAGGACC ACAAGTAGTG TGAGGCGAGA TGATCCTGGA
    GAGTTCCGTG TTGGTCGTCT CAAGCACGAA AGAGTAAAAG TTCCACGTGG TGAATCACTG
    ATGGAGTGGG CCGAAAACGT CATGCAAATA CACGCAGATC GGAAATCAGT GCTTGAGGTT
    GAATTTTTAG GAGAAGAAGG AACTGGTCTG GGGCCCACAT TAGAGTTCTA TGCTCTGGTA
    GCAGCAGAAT TCCAGAGAAC TGACTTGGGG GCTTGGCTTT GTGATGACAA CTTTCCAGAT
    GATGAATCTC GTCATGTCGA TCTTGGAGGT GGATTGAAAC CTCCCGGATA CTACGTGCAG
    AGATCATGTG GACTGTTCAC AGCTCCATTT CCACAAGACA GTGACGAGCT TGAAAGGATC
    ACAAAACTCT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT
    GTGGACTTAC CTATTTCCAA ACCTTTCTTT AAACTTATGT GTATGGGGGA CATTAAAAGT
    AATATGAGCA AACTGATTTA TGAGTCACGA GGCGACAGAG ACCTACACTG CACTGAAAGT
    CAGTCTGAAG CCTCTACAGA AGAAGGGCAT GACTCACTCT CAGTAGGAAG CTTTGAGGAG
    GATTCAAAAT CAGAATTCAT TCTAGATCCC CCCAAACCAA AACCGCCAGC TTGGTTTAAT
    GGAATTTTAA CTTGGGAAGA CTTTGAACTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ATCTTGCCAT CAAGAGGCGC CAGATTTTAA GCAACAAAGG TCTTTCTGAA
    GACGAGAAGA ACACAAAATT ACAAGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT
    CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT
    GGTTTTACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGCATGC ATACGGGTAT TCAGAAACAG
    ATGGAAGCCT TCAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAAG AGGTCCAAAT GATTCTTTGT GGAAATCAGT CGCCATCCTG GGCAGCAGAG
    GATATTATTA ATTATACTGA ACCTAAGTTG GGTTATACAC GTGACAGCCC TGGCTTCCTT
    AGGTTCGTGC GGGTTTTATG TGGTATGTCT TCAGACGAGA GGAAAGCATT TCTGCAGTTT
    ACCACTGGTT GTTCAACTCT GCCTCCAGGG GGACTGGCTA ACTTGCATCC CAGGCTCACA
    GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTACCCAT CAGTGAACAC ATGTGTGCAT
    TATCTAAAGT TGCCAGAATA CTCTTCGGAG GAGATCATGA GAGAGCGTCT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu56864
    Clone ID Related Accession (Same CDS sequence) XM_025271338.1
    Accession Version XM_025271338.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCCG GTACTACATC GACAGTGACT ATGTCAACAT CCAGTGTTAC TAGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAGT CTTTAAGTAA TACTTTAACC
    ACCAGCCTCA CATCAACCTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTGTTAG CTGAGCTAGA TGACGATGAG
    GACTTGCCAG AGCCAGACGA GGAAGATGAT GAAAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGT CCGTCCCTAC AGCGCCGAGC TGGCTCCCGC
    TCTGATGTAA CACACCATGC TGTCACCTCA CAGCTACCAC AGGTCCCAGC AGGAGCGGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAGGAA GAGTACGAAA CCAAGGGGGG CCGCCGGAGA
    ACATGGGACG ATGATTATGT GCTCAAGCGG CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGAACTAA TGTCCAGCAG ACAACTGATC TAGAAATTCC ACCCCCAGGA
    ACACCTCATT CAGAGCTTTT GGAAGAAGTT GAATGTACTC CATCACCTCG ATTAGCCCTC
    ACTTTAAAAG TAACAGGTCT TGGAACAACT CGTGAAGTTG AATTACCACT CACTAATTTC
    AGATCAACCA TCTTTTACTA TGTACAGAAA TTGCTTCAGT TGTCATGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCAACATACA CAATTATGTA CAGAGAAATG
    AAGGATTCTG ACAAAGAAAA GGAAAATGGA AAAATGGGTT GTTGGTCTAT AGAGCATGTG
    GAACAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG
    AATGCAGACG CCGCTTTCCT GCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG
    AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA
    AAGTCTGGGT TAAACCAGGG GGCCATTTCT ACTCTTCAAA GTAGTGATAT TCTGAATTTG
    ACAAAAGAAC AACCTCAGGC CAAAGCAGGC AATGGGCAGA ATTCATGTGG CGTGGAAGAC
    GTCCTTCAGC TCCTGCGTAT TCTGTATATA GTTGCAAGTG ACCCTTACTC AAGAATATCC
    CAGGAAGAAG GTGATGAGCA GCCTCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA
    AAAATCACAA CAAAAATTTT GCAGCAAATT GAGGAACCAT TGGCATTGGC AAGTGGAGCT
    CTGCCAGATT GGTGTGAACA GTTAACCAGC AAGTGTCCTT TTCTAATACC GTTCGAAACT
    AGACAGCTTT ATTTCACATG TACAGCATTT GGTGCATCAA GAGCAATTGT GTGGCTACAA
    AACCGACGTG AGGCCACTGT GGAAAGAACA AGGACCACAA GTAGTGTGAG GCGAGATGAT
    CCTGGAGAGT TCCGTGTTGG TCGTCTCAAG CACGAAAGAG TAAAAGTTCC ACGTGGTGAA
    TCACTGATGG AGTGGGCCGA AAACGTCATG CAAATACACG CAGATCGGAA ATCAGTGCTT
    GAGGTTGAAT TTTTAGGAGA AGAAGGAACT GGTCTGGGGC CCACATTAGA GTTCTATGCT
    CTGGTAGCAG CAGAATTCCA GAGAACTGAC TTGGGGGCTT GGCTTTGTGA TGACAACTTT
    CCAGATGATG AATCTCGTCA TGTCGATCTT GGAGGTGGAT TGAAACCTCC CGGATACTAC
    GTGCAGAGAT CATGTGGACT GTTCACAGCT CCATTTCCAC AAGACAGTGA CGAGCTTGAA
    AGGATCACAA AACTCTTTCA TTTCCTTGGA ATTTTCTTGG CCAAATGCAT TCAAGACAAT
    AGACTTGTGG ACTTACCTAT TTCCAAACCT TTCTTTAAAC TTATGTGTAT GGGGGACATT
    AAAAGTAATA TGAGCAAACT GATTTATGAG TCACGAGGCG ACAGAGACCT ACACTGCACT
    GAAAGTCAGT CTGAAGCCTC TACAGAAGAA GGGCATGACT CACTCTCAGT AGGAAGCTTT
    GAGGAGGATT CAAAATCAGA ATTCATTCTA GATCCCCCCA AACCAAAACC GCCAGCTTGG
    TTTAATGGAA TTTTAACTTG GGAAGACTTT GAACTAGTAA ACCCACACAG AGCCAGATTT
    TTAAAAGAAA TTAAAGATCT TGCCATCAAG AGGCGCCAGA TTTTAAGCAA CAAAGGTCTT
    TCTGAAGACG AGAAGAACAC AAAATTACAA GAACTAGTGC TGAAGAATCC ATCAGGTTCT
    GGGCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA
    ATATATGGTT TTACAGCTGT GGATCTCAAG CCAAGTGGTG AAGATGAGAT GATAACAATG
    GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GCATGCATAC GGGTATTCAG
    AAACAGATGG AAGCCTTCAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT
    TCCTTCAGCC ATGAAGAGGT CCAAATGATT CTTTGTGGAA ATCAGTCGCC ATCCTGGGCA
    GCAGAGGATA TTATTAATTA TACTGAACCT AAGTTGGGTT ATACACGTGA CAGCCCTGGC
    TTCCTTAGGT TCGTGCGGGT TTTATGTGGT ATGTCTTCAG ACGAGAGGAA AGCATTTCTG
    CAGTTTACCA CTGGTTGTTC AACTCTGCCT CCAGGGGGAC TGGCTAACTT GCATCCCAGG
    CTCACAGTTG TACGCAAGGT TGATGCTACT GATGCAAGCT ACCCATCAGT GAACACATGT
    GTGCATTATC TAAAGTTGCC AGAATACTCT TCGGAGGAGA TCATGAGAGA GCGTCTGCTA
    GCTGCTACAA TGGAGAAAGG CTTTCATCTC AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025127123.1
    CDS484..7479
    Translation

    Target ORF information:

    RefSeq Version XM_025271338.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HECT domain E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025271338.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTGC AGATGGGACA AGGAGATGAA 
    AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC
    GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTG TTAGAGGTAA CAGCCCGTGC CATAACATAC
    TACCTGGATG TATCTGCAGA ATGCACCCGA AGGATTGTTG GGGTAGATGG AGCTATAAAA
    GCACTTTGCA ATCGTTTGGT TGTGGTTGAA CTTAACAACA GAACAAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTTTT AGAACTAATA TGCACTCGTG AATCAGGAGC AGTCTTCGAG
    GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGATA GTGGACACCT GGTTCACAAA
    GACACCTTGC ACTCTGCCAT GGCTGTGGTG TCAAGACTCT GTGGCAAAAT GGAACCTCAA
    GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT
    CAGGTTTCAG ATGGAGCTCT GCGATGTTTT GCGTCACTAG CTGACCGATT TACCCGTCGT
    GGTGTTGACC CAGCTCCATT AGCCAAGCAC GGGTTAACTG AGGAGCTATT ATCTAGAATG
    GCTGCTGCTG GTGGTACTAT ATCAGGGCCA TCATCAGCTT GCAAACCCAG TCGCAGCACC
    ACAGGAGCAC CTTCCACAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCAGTAGTAA CGCACGATCT CCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTG CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTCGAAG GACGAAAGGC TTTGCCAAAA
    TCTAGTGCTG GATCCACAGG CAGAATCCCA GGACTTCGGA GACTGGATAG TTCTGGGGAG
    CGCTCACATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC ACTTATAGAT
    GCAATCGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA AACTCTATTA
    AACTGGGCTT CTGCATTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA AAGAGGTGCT
    GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCAG ATCTGAGAGA TGAAGATGGG
    AAAACTCCAT TAGATAAAGC TCGTGAAAGG GGCCATAGTG AAGTAGTAGC TATTCTTCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAGAAAAA GAAAGATACA
    AATAAAGATG AAGAAGAATG TAATGAGCCG AAAGGAGATC CAGAAATGGC ACCTATATAC
    TTGAAAAGGT TACTGCCAGT ATTTGCACAA ACATTTCAAC AGACTATGCT GCCTTCAATA
    AGGAAAGCAA GTCTTGCTCT GATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTCTTA
    AAAGAAGTTT GTGATTCTGA TGTTGGTCAC AATTTGCCTA CAGTACTAGT AGAAATCACT
    GCAACTGTTC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAAATCATA
    AGGGATTTAG TAGATAAAGG TGGCGATATA TTCTTGGATC AGCTAGCCAG ACTTGGTGTT
    ATTAGCAAAG TGTCCACTTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AAGAAGATGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGTAAACCA
    TATCATTGGA GAGATTGGTC CATCATTAGG GGAAGGGACT GCTTGTATAT ATGGAGTGAT
    GCAGCAGCCT TGGAATTGTC TAATGGCAGT AACGGATGGT TTAGATTTAT CTTGGATGGA
    AAACTAGCCA CCATGTATTC AAGTGGTAGT CCCGAAGGTG GATCGGATAG TTCAGAAAGT
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCACGAGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTCTCAGC ACCAGGACCC ACTAAACTCA CCGTAGGGAA TTGGTCACTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGCCAGCA AGCAACAGTA
    TTGAAAGAAG ATTTACCTGG ATTTGTGTTT GAATCTAATA GAGGAACCAG GCATTCATTT
    ACTGCAGAAA CTTCTCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGAAGA
    AAACTAAAAT CTAAATTAGA AAAAACTAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACCCTCAGA
    AACATAGCAA CTCAGTTAGA GTCATCTTGG GAACTTCATA CAAATAGACA ATGTATTGAG
    AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACAGCTTTAG AAAATCTGAT TGTACTTTTG
    AAGGATGAAA ACACAATTTC TCCATATGAG ATGTGCAGTA GTGGCTTGGT GCAAGCGCTT
    CTTACTGTTT TAAACAATAG CATGGATTTA GATATGAAAC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTTTTTAA AACAGCCTTT AGTGAAAATG AAGATGATGA AAGCCGACCA
    GCCGTTGCAT TAATCCGAAA GTTAATAGCT GTACTAGAGT CTATTGAACG TCTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACTTAT AACCTCCAGA TACTTACAAG AAGATTAAGA
    TTTCGGTTGG AACGTGCTCC TGGTGAAACT GCATTAATAG ACAGGACTGG CAGAATGTTG
    AAGATGGAAC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTCTTAAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTG TATTTCGACA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CCAAAACTGC ATATGAATGG GTTAATCCAG CTGCTTATGG ACTTGTAGTA
    GTAACATCAT CAGAAGGACG CAATCTACCT TATGGCCGCT TAGAAGACAT ACTGAGTCGT
    GACAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT
    CTGGGTCTTT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACAGCCAC CTGGCCTCTT
    GATCCACCGA AGGATGAGAA ACAAGGGTGG CGACATGTGA GAATTAAACA GATGGGAAAG
    AATGCAAGTG GACAGACCCA CTATCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA
    AATGGAGTAT GTGAAGATCA GCTAGGAAAA GCAGCTAAGG AAGCAGAAGC TAATCTTAGA
    CGACAGAGAC GTCTAGTTCG CTCCCAGGTA CTGAAGTACA TGGTTCCAGG GGCTCGTGTG
    ATCAGAGGCC TTGATTGGAA ATGGCGTGAT CAGGATGGCA GCCCACAGGG AGAAGGCACC
    GTCACAGGAG AACTGCATAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCCG GTACTACATC GACAGTGACT ATGTCAACAT CCAGTGTTAC TAGCAGCAGC
    AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAGT CTTTAAGTAA TACTTTAACC
    ACCAGCCTCA CATCAACCTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA
    TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTGTTAG CTGAGCTAGA TGACGATGAG
    GACTTGCCAG AGCCAGACGA GGAAGATGAT GAAAATGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGT CCGTCCCTAC AGCGCCGAGC TGGCTCCCGC
    TCTGATGTAA CACACCATGC TGTCACCTCA CAGCTACCAC AGGTCCCAGC AGGAGCGGGG
    AGCCGACCTA TTGGGGAGCA GGAAGAGGAA GAGTACGAAA CCAAGGGGGG CCGCCGGAGA
    ACATGGGACG ATGATTATGT GCTCAAGCGG CAGTTTTCAG CTTTGGTTCC TGCTTTTGAT
    CCTAGACCTG GTCGAACTAA TGTCCAGCAG ACAACTGATC TAGAAATTCC ACCCCCAGGA
    ACACCTCATT CAGAGCTTTT GGAAGAAGTT GAATGTACTC CATCACCTCG ATTAGCCCTC
    ACTTTAAAAG TAACAGGTCT TGGAACAACT CGTGAAGTTG AATTACCACT CACTAATTTC
    AGATCAACCA TCTTTTACTA TGTACAGAAA TTGCTTCAGT TGTCATGTAA TGGCAATGTG
    AAATCAGATA AACTTAGGCG TATTTGGGAG CCAACATACA CAATTATGTA CAGAGAAATG
    AAGGATTCTG ACAAAGAAAA GGAAAATGGA AAAATGGGTT GTTGGTCTAT AGAGCATGTG
    GAACAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG
    AATGCAGACG CCGCTTTCCT GCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG
    AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA
    AAGTCTGGGT TAAACCAGGG GGCCATTTCT ACTCTTCAAA GTAGTGATAT TCTGAATTTG
    ACAAAAGAAC AACCTCAGGC CAAAGCAGGC AATGGGCAGA ATTCATGTGG CGTGGAAGAC
    GTCCTTCAGC TCCTGCGTAT TCTGTATATA GTTGCAAGTG ACCCTTACTC AAGAATATCC
    CAGGAAGAAG GTGATGAGCA GCCTCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA
    AAAATCACAA CAAAAATTTT GCAGCAAATT GAGGAACCAT TGGCATTGGC AAGTGGAGCT
    CTGCCAGATT GGTGTGAACA GTTAACCAGC AAGTGTCCTT TTCTAATACC GTTCGAAACT
    AGACAGCTTT ATTTCACATG TACAGCATTT GGTGCATCAA GAGCAATTGT GTGGCTACAA
    AACCGACGTG AGGCCACTGT GGAAAGAACA AGGACCACAA GTAGTGTGAG GCGAGATGAT
    CCTGGAGAGT TCCGTGTTGG TCGTCTCAAG CACGAAAGAG TAAAAGTTCC ACGTGGTGAA
    TCACTGATGG AGTGGGCCGA AAACGTCATG CAAATACACG CAGATCGGAA ATCAGTGCTT
    GAGGTTGAAT TTTTAGGAGA AGAAGGAACT GGTCTGGGGC CCACATTAGA GTTCTATGCT
    CTGGTAGCAG CAGAATTCCA GAGAACTGAC TTGGGGGCTT GGCTTTGTGA TGACAACTTT
    CCAGATGATG AATCTCGTCA TGTCGATCTT GGAGGTGGAT TGAAACCTCC CGGATACTAC
    GTGCAGAGAT CATGTGGACT GTTCACAGCT CCATTTCCAC AAGACAGTGA CGAGCTTGAA
    AGGATCACAA AACTCTTTCA TTTCCTTGGA ATTTTCTTGG CCAAATGCAT TCAAGACAAT
    AGACTTGTGG ACTTACCTAT TTCCAAACCT TTCTTTAAAC TTATGTGTAT GGGGGACATT
    AAAAGTAATA TGAGCAAACT GATTTATGAG TCACGAGGCG ACAGAGACCT ACACTGCACT
    GAAAGTCAGT CTGAAGCCTC TACAGAAGAA GGGCATGACT CACTCTCAGT AGGAAGCTTT
    GAGGAGGATT CAAAATCAGA ATTCATTCTA GATCCCCCCA AACCAAAACC GCCAGCTTGG
    TTTAATGGAA TTTTAACTTG GGAAGACTTT GAACTAGTAA ACCCACACAG AGCCAGATTT
    TTAAAAGAAA TTAAAGATCT TGCCATCAAG AGGCGCCAGA TTTTAAGCAA CAAAGGTCTT
    TCTGAAGACG AGAAGAACAC AAAATTACAA GAACTAGTGC TGAAGAATCC ATCAGGTTCT
    GGGCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA
    ATATATGGTT TTACAGCTGT GGATCTCAAG CCAAGTGGTG AAGATGAGAT GATAACAATG
    GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GCATGCATAC GGGTATTCAG
    AAACAGATGG AAGCCTTCAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT
    TCCTTCAGCC ATGAAGAGGT CCAAATGATT CTTTGTGGAA ATCAGTCGCC ATCCTGGGCA
    GCAGAGGATA TTATTAATTA TACTGAACCT AAGTTGGGTT ATACACGTGA CAGCCCTGGC
    TTCCTTAGGT TCGTGCGGGT TTTATGTGGT ATGTCTTCAG ACGAGAGGAA AGCATTTCTG
    CAGTTTACCA CTGGTTGTTC AACTCTGCCT CCAGGGGGAC TGGCTAACTT GCATCCCAGG
    CTCACAGTTG TACGCAAGGT TGATGCTACT GATGCAAGCT ACCCATCAGT GAACACATGT
    GTGCATTATC TAAAGTTGCC AGAATACTCT TCGGAGGAGA TCATGAGAGA GCGTCTGCTA
    GCTGCTACAA TGGAGAAAGG CTTTCATCTC AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.