ORF » Species Summary » Bubalus bubalis » CHRNE cDNA ORF clone

CHRNE cDNA ORF clone, Bubalus bubalis(water buffalo)

The following CHRNE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu16560 XM_006060078.2
Latest version!		 Bubalus bubalis cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OBu16561 XM_006060079.1
Latest version!		 Bubalus bubalis cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OBu62076 XM_025280750.1
Latest version!		 Bubalus bubalis cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBu62074 XM_025280748.1
Latest version!		 Bubalus bubalis cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBu62075 XM_025280749.1
Latest version!		 Bubalus bubalis cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OBu16560 XM_006060078.1 Bubalus bubalis cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CHRNE ( XM_006060078.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006060140.1 Bubalus bubalis cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OBu16560
    Clone ID Related Accession (Same CDS sequence) XM_006060078.1 , XM_006060078.2
    Accession Version XM_006060078.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1476bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product acetylcholine receptor subunit epsilon isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784950.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCAGGGG CTCTGCTCTG CGCCTTGCTC CTCTTGCAGC TCCTCGGCAG AGGTGAGGGG 
    AAGAATGAGG AGCTGCGCCT TTACCACTAC CTCTTCGACA CCTATGACCC AGGACGCCGG 
    CCAGTGCAGG AGCCCGAGGA CACTGTCACC ATCTCCCTCA AAGTCACCCT GACCAACCTC 
    ATCTCCCTGA ACGAGAAAGA AGAGACCCTC ACCACCAGCG TCTGGATTGG AATCGACTGG 
    CAAGATTACC GACTCAACTA CAGCAAGGGC GACTTTGGGG GCGTAGAAAC CCTGCGGGTC 
    CCTTCAGAAC TCGTATGGCT GCCAGAGATT GTGCTGGAAA ACAATATCGA CGGCCAGTTC 
    GGGGTGGCCT ACGAGGCCAA TGTGCTGGTC TCCGAGGGCG GCTACATGAG CTGGTTGCCC 
    CCGGCCATCT ACCGCAGCAC CTGCGCCGTG GAGGTCACCT ACTTCCCTTT CGACTGGCAG 
    AACTGCTCGC TCGTTTTCCG CTCCCAGACG TACAATGCCG AAGAGGTGGA GTTTGTCTTT 
    GCGGTGGACG ACGAGGGCAG GACCATCAAC AAGATCGATA TCGATACTGA GGCCTATACT 
    GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCCGGGGTGA TCCGCCGCCA CGACGGTGAC 
    TCTGCGGGTG GCCCAGGGGA AACTGACGTC ATCTACTCGC TCATTATTCG CCGGAAGCCG 
    CTGTTCTACG TTATTAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC 
    GCTTACTTCC TGCCCGCGCA GGCTGGCGGC CAGAAATGCA CCGTTTCCAT CAACGTCCTG 
    CTCGCCCAGA CCGTCTTCTT GTTCCTGATT GCCCAGAAAA CCCCTGAGAC ATCTCTGAGC 
    GTGCCGCTGT TGGGCAGGTA CCTCATTTTC GTCATGGTGG TCGCCACACT CATTGTCATG 
    AATTGCGTCA TCGTGCTCAA CGTGTCATTG CGGACGCCCA CCACTCACGC CATGTCCCCG 
    CGGCTGCGCC ACGTGTTGCT GGAGCTGCTG CCCCAGCTCC TGGGCTCCGG CGCGCCCCCC 
    GAGATCCCCC GAGCTGCCTC GCCCCCAAGG AGGGCGTCGT CCCTGGGCCT CCTGCTCCGC 
    GCAGAGGAGC TGATACTGAA AAAGCCGCGG AGCGAACTTG TATTTGAGCA GCAGAGGCAC 
    CGGCACGGAA CCTGGACCGC TACCCTCTGC CAGAACCTGG GCGCTGCCGC CCCCGAGATC 
    CGCTGCTGTG TGGATGCCGT GAACTTCGTG GCTTCAAGCA CGCGGGACCA GGAAGCCACT 
    GGCGAGGAGG TGTCCGACTG GGTGCGCATG GGGAAGGCCC TTGACAGCAT CTGCTTCTGG 
    GCTGCTCTGG TGCTCTTCCT CGTGGGCTCC AGCCTCATCT TCCTCGGGGC CTACTTCAAC 
    CGAGTGCCCC AGCTGCCCTA CCCGCCATGT ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006060140.1
    CDS136..1611
    Translation

    Target ORF information:

    RefSeq Version XM_006060078.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006060078.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCAGGGG CTCTGCTCTG CGCCTTGCTC CTCTTGCAGC TCCTCGGCAG AGGTGAGGGG 
    AAGAATGAGG AGCTGCGCCT TTACCACTAC CTCTTCGACA CCTATGACCC AGGACGCCGG 
    CCAGTGCAGG AGCCCGAGGA CACTGTCACC ATCTCCCTCA AAGTCACCCT GACCAACCTC 
    ATCTCCCTGA ACGAGAAAGA AGAGACCCTC ACCACCAGCG TCTGGATTGG AATCGACTGG 
    CAAGATTACC GACTCAACTA CAGCAAGGGC GACTTTGGGG GCGTAGAAAC CCTGCGGGTC 
    CCTTCAGAAC TCGTATGGCT GCCAGAGATT GTGCTGGAAA ACAATATCGA CGGCCAGTTC 
    GGGGTGGCCT ACGAGGCCAA TGTGCTGGTC TCCGAGGGCG GCTACATGAG CTGGTTGCCC 
    CCGGCCATCT ACCGCAGCAC CTGCGCCGTG GAGGTCACCT ACTTCCCTTT CGACTGGCAG 
    AACTGCTCGC TCGTTTTCCG CTCCCAGACG TACAATGCCG AAGAGGTGGA GTTTGTCTTT 
    GCGGTGGACG ACGAGGGCAG GACCATCAAC AAGATCGATA TCGATACTGA GGCCTATACT 
    GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCCGGGGTGA TCCGCCGCCA CGACGGTGAC 
    TCTGCGGGTG GCCCAGGGGA AACTGACGTC ATCTACTCGC TCATTATTCG CCGGAAGCCG 
    CTGTTCTACG TTATTAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC 
    GCTTACTTCC TGCCCGCGCA GGCTGGCGGC CAGAAATGCA CCGTTTCCAT CAACGTCCTG 
    CTCGCCCAGA CCGTCTTCTT GTTCCTGATT GCCCAGAAAA CCCCTGAGAC ATCTCTGAGC 
    GTGCCGCTGT TGGGCAGGTA CCTCATTTTC GTCATGGTGG TCGCCACACT CATTGTCATG 
    AATTGCGTCA TCGTGCTCAA CGTGTCATTG CGGACGCCCA CCACTCACGC CATGTCCCCG 
    CGGCTGCGCC ACGTGTTGCT GGAGCTGCTG CCCCAGCTCC TGGGCTCCGG CGCGCCCCCC 
    GAGATCCCCC GAGCTGCCTC GCCCCCAAGG AGGGCGTCGT CCCTGGGCCT CCTGCTCCGC 
    GCAGAGGAGC TGATACTGAA AAAGCCGCGG AGCGAACTTG TATTTGAGCA GCAGAGGCAC 
    CGGCACGGAA CCTGGACCGC TACCCTCTGC CAGAACCTGG GCGCTGCCGC CCCCGAGATC 
    CGCTGCTGTG TGGATGCCGT GAACTTCGTG GCTTCAAGCA CGCGGGACCA GGAAGCCACT 
    GGCGAGGAGG TGTCCGACTG GGTGCGCATG GGGAAGGCCC TTGACAGCAT CTGCTTCTGG 
    GCTGCTCTGG TGCTCTTCCT CGTGGGCTCC AGCCTCATCT TCCTCGGGGC CTACTTCAAC 
    CGAGTGCCCC AGCTGCCCTA CCCGCCATGT ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CHRNE ( XM_006060078.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006060140.1 Bubalus bubalis cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OBu16560
    Clone ID Related Accession (Same CDS sequence) XM_006060078.1 , XM_006060078.2
    Accession Version XM_006060078.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1476bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product acetylcholine receptor subunit epsilon isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006060078.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCAGGGG CTCTGCTCTG CGCCTTGCTC CTCTTGCAGC TCCTCGGCAG AGGTGAGGGG 
    AAGAATGAGG AGCTGCGCCT TTACCACTAC CTCTTCGACA CCTATGACCC AGGACGCCGG 
    CCAGTGCAGG AGCCCGAGGA CACTGTCACC ATCTCCCTCA AAGTCACCCT GACCAACCTC 
    ATCTCCCTGA ACGAGAAAGA AGAGACCCTC ACCACCAGCG TCTGGATTGG AATCGACTGG 
    CAAGATTACC GACTCAACTA CAGCAAGGGC GACTTTGGGG GCGTAGAAAC CCTGCGGGTC 
    CCTTCAGAAC TCGTATGGCT GCCAGAGATT GTGCTGGAAA ACAATATCGA CGGCCAGTTC 
    GGGGTGGCCT ACGAGGCCAA TGTGCTGGTC TCCGAGGGCG GCTACATGAG CTGGTTGCCC 
    CCGGCCATCT ACCGCAGCAC CTGCGCCGTG GAGGTCACCT ACTTCCCTTT CGACTGGCAG 
    AACTGCTCGC TCGTTTTCCG CTCCCAGACG TACAATGCCG AAGAGGTGGA GTTTGTCTTT 
    GCGGTGGACG ACGAGGGCAG GACCATCAAC AAGATCGATA TCGATACTGA GGCCTATACT 
    GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCCGGGGTGA TCCGCCGCCA CGACGGTGAC 
    TCTGCGGGTG GCCCAGGGGA AACTGACGTC ATCTACTCGC TCATTATTCG CCGGAAGCCG 
    CTGTTCTACG TTATTAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC 
    GCTTACTTCC TGCCCGCGCA GGCTGGCGGC CAGAAATGCA CCGTTTCCAT CAACGTCCTG 
    CTCGCCCAGA CCGTCTTCTT GTTCCTGATT GCCCAGAAAA CCCCTGAGAC ATCTCTGAGC 
    GTGCCGCTGT TGGGCAGGTA CCTCATTTTC GTCATGGTGG TCGCCACACT CATTGTCATG 
    AATTGCGTCA TCGTGCTCAA CGTGTCATTG CGGACGCCCA CCACTCACGC CATGTCCCCG 
    CGGCTGCGCC ACGTGTTGCT GGAGCTGCTG CCCCAGCTCC TGGGCTCCGG CGCGCCCCCC 
    GAGATCCCCC GAGCTGCCTC GCCCCCAAGG AGGGCGTCGT CCCTGGGCCT CCTGCTCCGC 
    GCAGAGGAGC TGATACTGAA AAAGCCGCGG AGCGAACTTG TATTTGAGCA GCAGAGGCAC 
    CGGCACGGAA CCTGGACCGC TACCCTCTGC CAGAACCTGG GCGCTGCCGC CCCCGAGATC 
    CGCTGCTGTG TGGATGCCGT GAACTTCGTG GCTTCAAGCA CGCGGGACCA GGAAGCCACT 
    GGCGAGGAGG TGTCCGACTG GGTGCGCATG GGGAAGGCCC TTGACAGCAT CTGCTTCTGG 
    GCTGCTCTGG TGCTCTTCCT CGTGGGCTCC AGCCTCATCT TCCTCGGGGC CTACTTCAAC 
    CGAGTGCCCC AGCTGCCCTA CCCGCCATGT ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006060140.1
    CDS230..1705
    Translation

    Target ORF information:

    RefSeq Version XM_006060078.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006060078.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCAGGGG CTCTGCTCTG CGCCTTGCTC CTCTTGCAGC TCCTCGGCAG AGGTGAGGGG 
    AAGAATGAGG AGCTGCGCCT TTACCACTAC CTCTTCGACA CCTATGACCC AGGACGCCGG 
    CCAGTGCAGG AGCCCGAGGA CACTGTCACC ATCTCCCTCA AAGTCACCCT GACCAACCTC 
    ATCTCCCTGA ACGAGAAAGA AGAGACCCTC ACCACCAGCG TCTGGATTGG AATCGACTGG 
    CAAGATTACC GACTCAACTA CAGCAAGGGC GACTTTGGGG GCGTAGAAAC CCTGCGGGTC 
    CCTTCAGAAC TCGTATGGCT GCCAGAGATT GTGCTGGAAA ACAATATCGA CGGCCAGTTC 
    GGGGTGGCCT ACGAGGCCAA TGTGCTGGTC TCCGAGGGCG GCTACATGAG CTGGTTGCCC 
    CCGGCCATCT ACCGCAGCAC CTGCGCCGTG GAGGTCACCT ACTTCCCTTT CGACTGGCAG 
    AACTGCTCGC TCGTTTTCCG CTCCCAGACG TACAATGCCG AAGAGGTGGA GTTTGTCTTT 
    GCGGTGGACG ACGAGGGCAG GACCATCAAC AAGATCGATA TCGATACTGA GGCCTATACT 
    GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCCGGGGTGA TCCGCCGCCA CGACGGTGAC 
    TCTGCGGGTG GCCCAGGGGA AACTGACGTC ATCTACTCGC TCATTATTCG CCGGAAGCCG 
    CTGTTCTACG TTATTAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC 
    GCTTACTTCC TGCCCGCGCA GGCTGGCGGC CAGAAATGCA CCGTTTCCAT CAACGTCCTG 
    CTCGCCCAGA CCGTCTTCTT GTTCCTGATT GCCCAGAAAA CCCCTGAGAC ATCTCTGAGC 
    GTGCCGCTGT TGGGCAGGTA CCTCATTTTC GTCATGGTGG TCGCCACACT CATTGTCATG 
    AATTGCGTCA TCGTGCTCAA CGTGTCATTG CGGACGCCCA CCACTCACGC CATGTCCCCG 
    CGGCTGCGCC ACGTGTTGCT GGAGCTGCTG CCCCAGCTCC TGGGCTCCGG CGCGCCCCCC 
    GAGATCCCCC GAGCTGCCTC GCCCCCAAGG AGGGCGTCGT CCCTGGGCCT CCTGCTCCGC 
    GCAGAGGAGC TGATACTGAA AAAGCCGCGG AGCGAACTTG TATTTGAGCA GCAGAGGCAC 
    CGGCACGGAA CCTGGACCGC TACCCTCTGC CAGAACCTGG GCGCTGCCGC CCCCGAGATC 
    CGCTGCTGTG TGGATGCCGT GAACTTCGTG GCTTCAAGCA CGCGGGACCA GGAAGCCACT 
    GGCGAGGAGG TGTCCGACTG GGTGCGCATG GGGAAGGCCC TTGACAGCAT CTGCTTCTGG 
    GCTGCTCTGG TGCTCTTCCT CGTGGGCTCC AGCCTCATCT TCCTCGGGGC CTACTTCAAC 
    CGAGTGCCCC AGCTGCCCTA CCCGCCATGT ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CHRNE ( XM_006060079.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006060141.1 Bubalus bubalis cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OBu16561
    Clone ID Related Accession (Same CDS sequence) XM_006060079.1
    Accession Version XM_006060079.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1461bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product acetylcholine receptor subunit epsilon isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784950.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACTAGTA TGGACTTTCT TTCCTGTACT GGCAGAGGTG AGGGGAAGAA TGAGGAGCTG 
    CGCCTTTACC ACTACCTCTT CGACACCTAT GACCCAGGAC GCCGGCCAGT GCAGGAGCCC 
    GAGGACACTG TCACCATCTC CCTCAAAGTC ACCCTGACCA ACCTCATCTC CCTGAACGAG 
    AAAGAAGAGA CCCTCACCAC CAGCGTCTGG ATTGGAATCG ACTGGCAAGA TTACCGACTC 
    AACTACAGCA AGGGCGACTT TGGGGGCGTA GAAACCCTGC GGGTCCCTTC AGAACTCGTA 
    TGGCTGCCAG AGATTGTGCT GGAAAACAAT ATCGACGGCC AGTTCGGGGT GGCCTACGAG 
    GCCAATGTGC TGGTCTCCGA GGGCGGCTAC ATGAGCTGGT TGCCCCCGGC CATCTACCGC 
    AGCACCTGCG CCGTGGAGGT CACCTACTTC CCTTTCGACT GGCAGAACTG CTCGCTCGTT 
    TTCCGCTCCC AGACGTACAA TGCCGAAGAG GTGGAGTTTG TCTTTGCGGT GGACGACGAG 
    GGCAGGACCA TCAACAAGAT CGATATCGAT ACTGAGGCCT ATACTGAGAA CGGCGAGTGG 
    GCCATCGACT TCTGCCCCGG GGTGATCCGC CGCCACGACG GTGACTCTGC GGGTGGCCCA 
    GGGGAAACTG ACGTCATCTA CTCGCTCATT ATTCGCCGGA AGCCGCTGTT CTACGTTATT 
    AACATCATCG TGCCCTGCGT GCTCATCTCG GGCCTGGTGC TGCTCGCTTA CTTCCTGCCC 
    GCGCAGGCTG GCGGCCAGAA ATGCACCGTT TCCATCAACG TCCTGCTCGC CCAGACCGTC 
    TTCTTGTTCC TGATTGCCCA GAAAACCCCT GAGACATCTC TGAGCGTGCC GCTGTTGGGC 
    AGGTACCTCA TTTTCGTCAT GGTGGTCGCC ACACTCATTG TCATGAATTG CGTCATCGTG 
    CTCAACGTGT CATTGCGGAC GCCCACCACT CACGCCATGT CCCCGCGGCT GCGCCACGTG 
    TTGCTGGAGC TGCTGCCCCA GCTCCTGGGC TCCGGCGCGC CCCCCGAGAT CCCCCGAGCT 
    GCCTCGCCCC CAAGGAGGGC GTCGTCCCTG GGCCTCCTGC TCCGCGCAGA GGAGCTGATA 
    CTGAAAAAGC CGCGGAGCGA ACTTGTATTT GAGCAGCAGA GGCACCGGCA CGGAACCTGG 
    ACCGCTACCC TCTGCCAGAA CCTGGGCGCT GCCGCCCCCG AGATCCGCTG CTGTGTGGAT 
    GCCGTGAACT TCGTGGCTTC AAGCACGCGG GACCAGGAAG CCACTGGCGA GGAGGTGTCC 
    GACTGGGTGC GCATGGGGAA GGCCCTTGAC AGCATCTGCT TCTGGGCTGC TCTGGTGCTC 
    TTCCTCGTGG GCTCCAGCCT CATCTTCCTC GGGGCCTACT TCAACCGAGT GCCCCAGCTG 
    CCCTACCCGC CATGTATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006060141.1
    CDS38..1498
    Translation

    Target ORF information:

    RefSeq Version XM_006060079.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006060079.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACTAGTA TGGACTTTCT TTCCTGTACT GGCAGAGGTG AGGGGAAGAA TGAGGAGCTG 
    CGCCTTTACC ACTACCTCTT CGACACCTAT GACCCAGGAC GCCGGCCAGT GCAGGAGCCC 
    GAGGACACTG TCACCATCTC CCTCAAAGTC ACCCTGACCA ACCTCATCTC CCTGAACGAG 
    AAAGAAGAGA CCCTCACCAC CAGCGTCTGG ATTGGAATCG ACTGGCAAGA TTACCGACTC 
    AACTACAGCA AGGGCGACTT TGGGGGCGTA GAAACCCTGC GGGTCCCTTC AGAACTCGTA 
    TGGCTGCCAG AGATTGTGCT GGAAAACAAT ATCGACGGCC AGTTCGGGGT GGCCTACGAG 
    GCCAATGTGC TGGTCTCCGA GGGCGGCTAC ATGAGCTGGT TGCCCCCGGC CATCTACCGC 
    AGCACCTGCG CCGTGGAGGT CACCTACTTC CCTTTCGACT GGCAGAACTG CTCGCTCGTT 
    TTCCGCTCCC AGACGTACAA TGCCGAAGAG GTGGAGTTTG TCTTTGCGGT GGACGACGAG 
    GGCAGGACCA TCAACAAGAT CGATATCGAT ACTGAGGCCT ATACTGAGAA CGGCGAGTGG 
    GCCATCGACT TCTGCCCCGG GGTGATCCGC CGCCACGACG GTGACTCTGC GGGTGGCCCA 
    GGGGAAACTG ACGTCATCTA CTCGCTCATT ATTCGCCGGA AGCCGCTGTT CTACGTTATT 
    AACATCATCG TGCCCTGCGT GCTCATCTCG GGCCTGGTGC TGCTCGCTTA CTTCCTGCCC 
    GCGCAGGCTG GCGGCCAGAA ATGCACCGTT TCCATCAACG TCCTGCTCGC CCAGACCGTC 
    TTCTTGTTCC TGATTGCCCA GAAAACCCCT GAGACATCTC TGAGCGTGCC GCTGTTGGGC 
    AGGTACCTCA TTTTCGTCAT GGTGGTCGCC ACACTCATTG TCATGAATTG CGTCATCGTG 
    CTCAACGTGT CATTGCGGAC GCCCACCACT CACGCCATGT CCCCGCGGCT GCGCCACGTG 
    TTGCTGGAGC TGCTGCCCCA GCTCCTGGGC TCCGGCGCGC CCCCCGAGAT CCCCCGAGCT 
    GCCTCGCCCC CAAGGAGGGC GTCGTCCCTG GGCCTCCTGC TCCGCGCAGA GGAGCTGATA 
    CTGAAAAAGC CGCGGAGCGA ACTTGTATTT GAGCAGCAGA GGCACCGGCA CGGAACCTGG 
    ACCGCTACCC TCTGCCAGAA CCTGGGCGCT GCCGCCCCCG AGATCCGCTG CTGTGTGGAT 
    GCCGTGAACT TCGTGGCTTC AAGCACGCGG GACCAGGAAG CCACTGGCGA GGAGGTGTCC 
    GACTGGGTGC GCATGGGGAA GGCCCTTGAC AGCATCTGCT TCTGGGCTGC TCTGGTGCTC 
    TTCCTCGTGG GCTCCAGCCT CATCTTCCTC GGGGCCTACT TCAACCGAGT GCCCCAGCTG 
    CCCTACCCGC CATGTATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CHRNE ( XM_025280750.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025136535.1 Bubalus bubalis cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OBu62076
    Clone ID Related Accession (Same CDS sequence) XM_025280750.1
    Accession Version XM_025280750.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product acetylcholine receptor subunit epsilon isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGCTGGT TGCCCCCGGC CATCTACCGC AGCACCTGCG CCGTGGAGGT CACCTACTTC 
    CCTTTCGACT GGCAGAACTG CTCGCTCGTT TTCCGCTCCC AGACGTACAA TGCCGAAGAG 
    GTGGAGTTTG TCTTTGCGGT GGACGACGAG GGCAGGACCA TCAACAAGAT CGATATCGAT 
    ACTGAGGCCT ATACTGAGAA CGGCGAGTGG GCCATCGACT TCTGCCCCGG GGTGATCCGC 
    CGCCACGACG GTGACTCTGC GGGTGGCCCA GGGGAAACTG ACGTCATCTA CTCGCTCATT 
    ATTCGCCGGA AGCCGCTGTT CTACGTTATT AACATCATCG TGCCCTGCGT GCTCATCTCG 
    GGCCTGGTGC TGCTCGCTTA CTTCCTGCCC GCGCAGGCTG GCGGCCAGAA ATGCACCGTT 
    TCCATCAACG TCCTGCTCGC CCAGACCGTC TTCTTGTTCC TGATTGCCCA GAAAACCCCT 
    GAGACATCTC TGAGCGTGCC GCTGTTGGGC AGGGAACTGG GAGTCCCCTT CATGGTTGCC 
    ACTGGCCGTG CGAGGGCGGG GAGAAAGACC AGGCCGGGGG GCGGACCGTC TGGCCCTGCC 
    GGGAGGAGCA GCTCCACGCC TGCGGAGAGG TACCTCATTT TCGTCATGGT GGTCGCCACA 
    CTCATTGTCA TGAATTGCGT CATCGTGCTC AACGTGTCAT TGCGGACGCC CACCACTCAC 
    GCCATGTCCC CGCGGCTGCG CCACGTGTTG CTGGAGCTGC TGCCCCAGCT CCTGGGCTCC 
    GGCGCGCCCC CCGAGATCCC CCGAGCTGCC TCGCCCCCAA GGAGGGCGTC GTCCCTGGGC 
    CTCCTGCTCC GCGCAGAGGA GCTGATACTG AAAAAGCCGC GGAGCGAACT TGTATTTGAG 
    CAGCAGAGGC ACCGGCACGG AACCTGGACC GCTACCCTCT GCCAGAACCT GGGCGCTGCC 
    GCCCCCGAGA TCCGCTGCTG TGTGGATGCC GTGAACTTCG TGGCTTCAAG CACGCGGGAC 
    CAGGAAGCCA CTGGCGAGGA GGTGTCCGAC TGGGTGCGCA TGGGGAAGGC CCTTGACAGC 
    ATCTGCTTCT GGGCTGCTCT GGTGCTCTTC CTCGTGGGCT CCAGCCTCAT CTTCCTCGGG 
    GCCTACTTCA ACCGAGTGCC CCAGCTGCCC TACCCGCCAT GTATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025136535.1
    CDS179..1366
    Translation

    Target ORF information:

    RefSeq Version XM_025280750.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025280750.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGCTGGT TGCCCCCGGC CATCTACCGC AGCACCTGCG CCGTGGAGGT CACCTACTTC 
    CCTTTCGACT GGCAGAACTG CTCGCTCGTT TTCCGCTCCC AGACGTACAA TGCCGAAGAG 
    GTGGAGTTTG TCTTTGCGGT GGACGACGAG GGCAGGACCA TCAACAAGAT CGATATCGAT 
    ACTGAGGCCT ATACTGAGAA CGGCGAGTGG GCCATCGACT TCTGCCCCGG GGTGATCCGC 
    CGCCACGACG GTGACTCTGC GGGTGGCCCA GGGGAAACTG ACGTCATCTA CTCGCTCATT 
    ATTCGCCGGA AGCCGCTGTT CTACGTTATT AACATCATCG TGCCCTGCGT GCTCATCTCG 
    GGCCTGGTGC TGCTCGCTTA CTTCCTGCCC GCGCAGGCTG GCGGCCAGAA ATGCACCGTT 
    TCCATCAACG TCCTGCTCGC CCAGACCGTC TTCTTGTTCC TGATTGCCCA GAAAACCCCT 
    GAGACATCTC TGAGCGTGCC GCTGTTGGGC AGGGAACTGG GAGTCCCCTT CATGGTTGCC 
    ACTGGCCGTG CGAGGGCGGG GAGAAAGACC AGGCCGGGGG GCGGACCGTC TGGCCCTGCC 
    GGGAGGAGCA GCTCCACGCC TGCGGAGAGG TACCTCATTT TCGTCATGGT GGTCGCCACA 
    CTCATTGTCA TGAATTGCGT CATCGTGCTC AACGTGTCAT TGCGGACGCC CACCACTCAC 
    GCCATGTCCC CGCGGCTGCG CCACGTGTTG CTGGAGCTGC TGCCCCAGCT CCTGGGCTCC 
    GGCGCGCCCC CCGAGATCCC CCGAGCTGCC TCGCCCCCAA GGAGGGCGTC GTCCCTGGGC 
    CTCCTGCTCC GCGCAGAGGA GCTGATACTG AAAAAGCCGC GGAGCGAACT TGTATTTGAG 
    CAGCAGAGGC ACCGGCACGG AACCTGGACC GCTACCCTCT GCCAGAACCT GGGCGCTGCC 
    GCCCCCGAGA TCCGCTGCTG TGTGGATGCC GTGAACTTCG TGGCTTCAAG CACGCGGGAC 
    CAGGAAGCCA CTGGCGAGGA GGTGTCCGAC TGGGTGCGCA TGGGGAAGGC CCTTGACAGC 
    ATCTGCTTCT GGGCTGCTCT GGTGCTCTTC CTCGTGGGCT CCAGCCTCAT CTTCCTCGGG 
    GCCTACTTCA ACCGAGTGCC CCAGCTGCCC TACCCGCCAT GTATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CHRNE ( XM_025280748.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025136533.1 Bubalus bubalis cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OBu62074
    Clone ID Related Accession (Same CDS sequence) XM_025280748.1
    Accession Version XM_025280748.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product acetylcholine receptor subunit epsilon isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGACACTC TGCTTTTGCC GAACTCTGCT GGCGGAAACT GCATTTGTGT GACCTTGGCT 
    GGGCTATGGG TGGTGCTGAG TGTAGCAGGC AAGGGATGTA ACCTGCGCAG GGCAGCTGAC 
    TCAGAGGATA CCAGTGCAGT GTTGAAAGCA GGACCTTCCA CACCCCCTCT GCCAGGCAGA 
    GGTGAGGGGA AGAATGAGGA GCTGCGCCTT TACCACTACC TCTTCGACAC CTATGACCCA 
    GGACGCCGGC CAGTGCAGGA GCCCGAGGAC ACTGTCACCA TCTCCCTCAA AGTCACCCTG 
    ACCAACCTCA TCTCCCTGAA CGAGAAAGAA GAGACCCTCA CCACCAGCGT CTGGATTGGA 
    ATCGACTGGC AAGATTACCG ACTCAACTAC AGCAAGGGCG ACTTTGGGGG CGTAGAAACC 
    CTGCGGGTCC CTTCAGAACT CGTATGGCTG CCAGAGATTG TGCTGGAAAA CAATATCGAC 
    GGCCAGTTCG GGGTGGCCTA CGAGGCCAAT GTGCTGGTCT CCGAGGGCGG CTACATGAGC 
    TGGTTGCCCC CGGCCATCTA CCGCAGCACC TGCGCCGTGG AGGTCACCTA CTTCCCTTTC 
    GACTGGCAGA ACTGCTCGCT CGTTTTCCGC TCCCAGACGT ACAATGCCGA AGAGGTGGAG 
    TTTGTCTTTG CGGTGGACGA CGAGGGCAGG ACCATCAACA AGATCGATAT CGATACTGAG 
    GCCTATACTG AGAACGGCGA GTGGGCCATC GACTTCTGCC CCGGGGTGAT CCGCCGCCAC 
    GACGGTGACT CTGCGGGTGG CCCAGGGGAA ACTGACGTCA TCTACTCGCT CATTATTCGC 
    CGGAAGCCGC TGTTCTACGT TATTAACATC ATCGTGCCCT GCGTGCTCAT CTCGGGCCTG 
    GTGCTGCTCG CTTACTTCCT GCCCGCGCAG GCTGGCGGCC AGAAATGCAC CGTTTCCATC 
    AACGTCCTGC TCGCCCAGAC CGTCTTCTTG TTCCTGATTG CCCAGAAAAC CCCTGAGACA 
    TCTCTGAGCG TGCCGCTGTT GGGCAGGTAC CTCATTTTCG TCATGGTGGT CGCCACACTC 
    ATTGTCATGA ATTGCGTCAT CGTGCTCAAC GTGTCATTGC GGACGCCCAC CACTCACGCC 
    ATGTCCCCGC GGCTGCGCCA CGTGTTGCTG GAGCTGCTGC CCCAGCTCCT GGGCTCCGGC 
    GCGCCCCCCG AGATCCCCCG AGCTGCCTCG CCCCCAAGGA GGGCGTCGTC CCTGGGCCTC 
    CTGCTCCGCG CAGAGGAGCT GATACTGAAA AAGCCGCGGA GCGAACTTGT ATTTGAGCAG 
    CAGAGGCACC GGCACGGAAC CTGGACCGCT ACCCTCTGCC AGAACCTGGG CGCTGCCGCC 
    CCCGAGATCC GCTGCTGTGT GGATGCCGTG AACTTCGTGG CTTCAAGCAC GCGGGACCAG 
    GAAGCCACTG GCGAGGAGGT GTCCGACTGG GTGCGCATGG GGAAGGCCCT TGACAGCATC 
    TGCTTCTGGG CTGCTCTGGT GCTCTTCCTC GTGGGCTCCA GCCTCATCTT CCTCGGGGCC 
    TACTTCAACC GAGTGCCCCA GCTGCCCTAC CCGCCATGTA TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025136533.1
    CDS222..1826
    Translation

    Target ORF information:

    RefSeq Version XM_025280748.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025280748.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGACACTC TGCTTTTGCC GAACTCTGCT GGCGGAAACT GCATTTGTGT GACCTTGGCT 
    GGGCTATGGG TGGTGCTGAG TGTAGCAGGC AAGGGATGTA ACCTGCGCAG GGCAGCTGAC 
    TCAGAGGATA CCAGTGCAGT GTTGAAAGCA GGACCTTCCA CACCCCCTCT GCCAGGCAGA 
    GGTGAGGGGA AGAATGAGGA GCTGCGCCTT TACCACTACC TCTTCGACAC CTATGACCCA 
    GGACGCCGGC CAGTGCAGGA GCCCGAGGAC ACTGTCACCA TCTCCCTCAA AGTCACCCTG 
    ACCAACCTCA TCTCCCTGAA CGAGAAAGAA GAGACCCTCA CCACCAGCGT CTGGATTGGA 
    ATCGACTGGC AAGATTACCG ACTCAACTAC AGCAAGGGCG ACTTTGGGGG CGTAGAAACC 
    CTGCGGGTCC CTTCAGAACT CGTATGGCTG CCAGAGATTG TGCTGGAAAA CAATATCGAC 
    GGCCAGTTCG GGGTGGCCTA CGAGGCCAAT GTGCTGGTCT CCGAGGGCGG CTACATGAGC 
    TGGTTGCCCC CGGCCATCTA CCGCAGCACC TGCGCCGTGG AGGTCACCTA CTTCCCTTTC 
    GACTGGCAGA ACTGCTCGCT CGTTTTCCGC TCCCAGACGT ACAATGCCGA AGAGGTGGAG 
    TTTGTCTTTG CGGTGGACGA CGAGGGCAGG ACCATCAACA AGATCGATAT CGATACTGAG 
    GCCTATACTG AGAACGGCGA GTGGGCCATC GACTTCTGCC CCGGGGTGAT CCGCCGCCAC 
    GACGGTGACT CTGCGGGTGG CCCAGGGGAA ACTGACGTCA TCTACTCGCT CATTATTCGC 
    CGGAAGCCGC TGTTCTACGT TATTAACATC ATCGTGCCCT GCGTGCTCAT CTCGGGCCTG 
    GTGCTGCTCG CTTACTTCCT GCCCGCGCAG GCTGGCGGCC AGAAATGCAC CGTTTCCATC 
    AACGTCCTGC TCGCCCAGAC CGTCTTCTTG TTCCTGATTG CCCAGAAAAC CCCTGAGACA 
    TCTCTGAGCG TGCCGCTGTT GGGCAGGTAC CTCATTTTCG TCATGGTGGT CGCCACACTC 
    ATTGTCATGA ATTGCGTCAT CGTGCTCAAC GTGTCATTGC GGACGCCCAC CACTCACGCC 
    ATGTCCCCGC GGCTGCGCCA CGTGTTGCTG GAGCTGCTGC CCCAGCTCCT GGGCTCCGGC 
    GCGCCCCCCG AGATCCCCCG AGCTGCCTCG CCCCCAAGGA GGGCGTCGTC CCTGGGCCTC 
    CTGCTCCGCG CAGAGGAGCT GATACTGAAA AAGCCGCGGA GCGAACTTGT ATTTGAGCAG 
    CAGAGGCACC GGCACGGAAC CTGGACCGCT ACCCTCTGCC AGAACCTGGG CGCTGCCGCC 
    CCCGAGATCC GCTGCTGTGT GGATGCCGTG AACTTCGTGG CTTCAAGCAC GCGGGACCAG 
    GAAGCCACTG GCGAGGAGGT GTCCGACTGG GTGCGCATGG GGAAGGCCCT TGACAGCATC 
    TGCTTCTGGG CTGCTCTGGT GCTCTTCCTC GTGGGCTCCA GCCTCATCTT CCTCGGGGCC 
    TACTTCAACC GAGTGCCCCA GCTGCCCTAC CCGCCATGTA TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CHRNE ( XM_025280749.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025136534.1 Bubalus bubalis cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OBu62075
    Clone ID Related Accession (Same CDS sequence) XM_025280749.1
    Accession Version XM_025280749.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product acetylcholine receptor subunit epsilon isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCAGGGG CTCTGCTCTG CGCCTTGCTC CTCTTGCAGC TCCTCGGCAG AGGTGAGGGG 
    AAGAATGAGG AGCTGCGCCT TTACCACTAC CTCTTCGACA CCTATGACCC AGGACGCCGG 
    CCAGTGCAGG AGCCCGAGGA CACTGTCACC ATCTCCCTCA AAGTCACCCT GACCAACCTC 
    ATCTCCCTGA ACGAGAAAGA AGAGACCCTC ACCACCAGCG TCTGGATTGG AATCGACTGG 
    CAAGATTACC GACTCAACTA CAGCAAGGGC GACTTTGGGG GCGTAGAAAC CCTGCGGGTC 
    CCTTCAGAAC TCGTATGGCT GCCAGAGATT GTGCTGGAAA ACAATATCGA CGGCCAGTTC 
    GGGGTGGCCT ACGAGGCCAA TGTGCTGGTC TCCGAGGGCG GCTACATGAG CTGGTTGCCC 
    CCGGCCATCT ACCGCAGCAC CTGCGCCGTG GAGGTCACCT ACTTCCCTTT CGACTGGCAG 
    AACTGCTCGC TCGTTTTCCG CTCCCAGACG TACAATGCCG AAGAGGTGGA GTTTGTCTTT 
    GCGGTGGACG ACGAGGGCAG GACCATCAAC AAGATCGATA TCGATACTGA GGCCTATACT 
    GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCCGGGGTGA TCCGCCGCCA CGACGGTGAC 
    TCTGCGGGTG GCCCAGGGGA AACTGACGTC ATCTACTCGC TCATTATTCG CCGGAAGCCG 
    CTGTTCTACG TTATTAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC 
    GCTTACTTCC TGCCCGCGCA GGCTGGCGGC CAGAAATGCA CCGTTTCCAT CAACGTCCTG 
    CTCGCCCAGA CCGTCTTCTT GTTCCTGATT GCCCAGAAAA CCCCTGAGAC ATCTCTGAGC 
    GTGCCGCTGT TGGGCAGGGA ACTGGGAGTC CCCTTCATGG TTGCCACTGG CCGTGCGAGG 
    GCGGGGAGAA AGACCAGGCC GGGGGGCGGA CCGTCTGGCC CTGCCGGGAG GAGCAGCTCC 
    ACGCCTGCGG AGAGGTACCT CATTTTCGTC ATGGTGGTCG CCACACTCAT TGTCATGAAT 
    TGCGTCATCG TGCTCAACGT GTCATTGCGG ACGCCCACCA CTCACGCCAT GTCCCCGCGG 
    CTGCGCCACG TGTTGCTGGA GCTGCTGCCC CAGCTCCTGG GCTCCGGCGC GCCCCCCGAG 
    ATCCCCCGAG CTGCCTCGCC CCCAAGGAGG GCGTCGTCCC TGGGCCTCCT GCTCCGCGCA 
    GAGGAGCTGA TACTGAAAAA GCCGCGGAGC GAACTTGTAT TTGAGCAGCA GAGGCACCGG 
    CACGGAACCT GGACCGCTAC CCTCTGCCAG AACCTGGGCG CTGCCGCCCC CGAGATCCGC 
    TGCTGTGTGG ATGCCGTGAA CTTCGTGGCT TCAAGCACGC GGGACCAGGA AGCCACTGGC 
    GAGGAGGTGT CCGACTGGGT GCGCATGGGG AAGGCCCTTG ACAGCATCTG CTTCTGGGCT 
    GCTCTGGTGC TCTTCCTCGT GGGCTCCAGC CTCATCTTCC TCGGGGCCTA CTTCAACCGA 
    GTGCCCCAGC TGCCCTACCC GCCATGTATG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025136534.1
    CDS234..1826
    Translation

    Target ORF information:

    RefSeq Version XM_025280749.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025280749.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCAGGGG CTCTGCTCTG CGCCTTGCTC CTCTTGCAGC TCCTCGGCAG AGGTGAGGGG 
    AAGAATGAGG AGCTGCGCCT TTACCACTAC CTCTTCGACA CCTATGACCC AGGACGCCGG 
    CCAGTGCAGG AGCCCGAGGA CACTGTCACC ATCTCCCTCA AAGTCACCCT GACCAACCTC 
    ATCTCCCTGA ACGAGAAAGA AGAGACCCTC ACCACCAGCG TCTGGATTGG AATCGACTGG 
    CAAGATTACC GACTCAACTA CAGCAAGGGC GACTTTGGGG GCGTAGAAAC CCTGCGGGTC 
    CCTTCAGAAC TCGTATGGCT GCCAGAGATT GTGCTGGAAA ACAATATCGA CGGCCAGTTC 
    GGGGTGGCCT ACGAGGCCAA TGTGCTGGTC TCCGAGGGCG GCTACATGAG CTGGTTGCCC 
    CCGGCCATCT ACCGCAGCAC CTGCGCCGTG GAGGTCACCT ACTTCCCTTT CGACTGGCAG 
    AACTGCTCGC TCGTTTTCCG CTCCCAGACG TACAATGCCG AAGAGGTGGA GTTTGTCTTT 
    GCGGTGGACG ACGAGGGCAG GACCATCAAC AAGATCGATA TCGATACTGA GGCCTATACT 
    GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCCGGGGTGA TCCGCCGCCA CGACGGTGAC 
    TCTGCGGGTG GCCCAGGGGA AACTGACGTC ATCTACTCGC TCATTATTCG CCGGAAGCCG 
    CTGTTCTACG TTATTAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC 
    GCTTACTTCC TGCCCGCGCA GGCTGGCGGC CAGAAATGCA CCGTTTCCAT CAACGTCCTG 
    CTCGCCCAGA CCGTCTTCTT GTTCCTGATT GCCCAGAAAA CCCCTGAGAC ATCTCTGAGC 
    GTGCCGCTGT TGGGCAGGGA ACTGGGAGTC CCCTTCATGG TTGCCACTGG CCGTGCGAGG 
    GCGGGGAGAA AGACCAGGCC GGGGGGCGGA CCGTCTGGCC CTGCCGGGAG GAGCAGCTCC 
    ACGCCTGCGG AGAGGTACCT CATTTTCGTC ATGGTGGTCG CCACACTCAT TGTCATGAAT 
    TGCGTCATCG TGCTCAACGT GTCATTGCGG ACGCCCACCA CTCACGCCAT GTCCCCGCGG 
    CTGCGCCACG TGTTGCTGGA GCTGCTGCCC CAGCTCCTGG GCTCCGGCGC GCCCCCCGAG 
    ATCCCCCGAG CTGCCTCGCC CCCAAGGAGG GCGTCGTCCC TGGGCCTCCT GCTCCGCGCA 
    GAGGAGCTGA TACTGAAAAA GCCGCGGAGC GAACTTGTAT TTGAGCAGCA GAGGCACCGG 
    CACGGAACCT GGACCGCTAC CCTCTGCCAG AACCTGGGCG CTGCCGCCCC CGAGATCCGC 
    TGCTGTGTGG ATGCCGTGAA CTTCGTGGCT TCAAGCACGC GGGACCAGGA AGCCACTGGC 
    GAGGAGGTGT CCGACTGGGT GCGCATGGGG AAGGCCCTTG ACAGCATCTG CTTCTGGGCT 
    GCTCTGGTGC TCTTCCTCGT GGGCTCCAGC CTCATCTTCC TCGGGGCCTA CTTCAACCGA 
    GTGCCCCAGC TGCCCTACCC GCCATGTATG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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