NIPBL cDNA ORF clone, Bubalus bubalis(water buffalo)

The following NIPBL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIPBL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu16138 XM_006059558.1
Latest version!
Bubalus bubalis Nipped-B homolog (Drosophila) (NIPBL), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBu53882 XM_006059558.2
Latest version!
Bubalus bubalis NIPBL, cohesin loading factor (NIPBL), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBu16136 XM_006059556.1
Latest version!
Bubalus bubalis Nipped-B homolog (Drosophila) (NIPBL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBu16137 XM_006059557.1
Latest version!
Bubalus bubalis Nipped-B homolog (Drosophila) (NIPBL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBu53879 XM_006059556.2
Latest version!
Bubalus bubalis NIPBL, cohesin loading factor (NIPBL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBu53880 XM_025270602.1
Latest version!
Bubalus bubalis NIPBL, cohesin loading factor (NIPBL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBu53881 XM_006059557.2
Latest version!
Bubalus bubalis NIPBL, cohesin loading factor (NIPBL), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu16138
    Clone ID Related Accession (Same CDS sequence) XM_006059558.1
    Accession Version XM_006059558.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product nipped-B-like protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784928.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGTCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCTACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAACGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGGTAGT
    ATAGATCAAT CAGTGTTAAA AGAATTACCC CCCGAACTCC TGGCAGAAAT TGAATCCACC
    ATGCCACTTT GTGAACGTGT GAAAATGAAT AAACGCAAGC GTAGCACAGT CAATGAAAAA
    CCAAAATATG CTGAAATCAG TTCAGATGAA GATAATGATA GTGATGAAAC TTTTGAATCC
    TCTAGGAAAC GACATAAAAA AGATGATGAT AAAGCTTGGG AATATGAAGA GCGTGACAGA
    AGAAGCTCTG GGGATCATAG GAGAAGTGGC CACTCTCATG AAGGAAGAAG GAGTTCAGGT
    GGTGGTCGTT ATCGAAACCG AAGCCCATCA GATTCTGACA TGGAAGATTA TTCTCCTCCT
    CCCAGCCTTA GTGAGGTCGC TAGAAAAATG AAGAAAAAAG AAAAACAGAA GAAGCGGAAA
    GCTTATGAAC CAAAACTAAC ACCCGAAGAA ATGATGGATT CTTCAACTTT TAAGAGATTC
    ACAGCCTCAA TAGAGAATAT TTTGGATAAC TTAGAAGATA TGGATTTTAC TGCATTTGGT
    GATGATGATG AAATTCCTCA GGAACTGCTC TTAGGAAAAC ATCAGCTTAA TGAACTTGGC
    AGTGAATCTG CTAAAATTAA AGCAATGGGT ATAATGGATA AGCTTTCCAC TGACAAAACT
    GTGAAAGTCC TAAATATCTT GGAAAAGAAT ATTCAAGATG GGTCAAAGCT CTCCACTTTG
    TTAAATCATA ATAACGATAC TGAAGAAGAA GAAAGGTTAT GGAGAGACCT TATTATGGAG
    AGAGTGACAA AATCAGCAGA TGCCTGTCTT ACAACTATAA ACATCATGAC ATCCCCTAAC
    ATGCCAAAAG CTGTATATAT TGAGGATGTA ATCGAAAGAG TTATACAGTA CACTAAATTT
    CATTTGCAGA ATACACTTTA TCCTCAGTAT GATCCTGTTT ACAGATTAGA TCCTCATGGA
    GGAGGCTTAC TAAGTTCAAA GGCAAAACGG GCTAAATGTT CTACCCATAA GCAGAGAGTA
    ATAGTAATGC TTTATAATAA AGTTTGTGAC ATTGTTAGCA GCTTATCAGA ACTACTAGAA
    ATACAACTTC TAACAGACAC AACAATCCTT CAGGTATCTT CAATGGGAAT AACACCATTT
    TTTGTGGAAA ATGTCAGTGA ACTACAATTG TGTGCCATTA AATTAGTCAC TGCAGTATTC
    TCAAGATATG AAAAACATAG GCAGTTAATT TTGGAAGAAA TTTTTACTTC ACTTGCAAGA
    TTACCTACCA GCAAGAGGAG CTTAAGGAAC TTCAGGTTAA ATAGTAGTGA TATGGATGGA
    GAGCCTATGT ATATTCAGAT GGTTACAGCA CTGGTTTTAC AACTGATTCA GTGTGTAGTA
    CACTTACCAT CATCAGAGAA GGACTCTAAT TCAGAAGAGG ACTCAAATAA AAAGGTTGAC
    CAGGATGTTG TCATTACCAA TTCTTATGAA ACAGCCATGC GAACAGCCCA AAACTTCCTT
    TCCATCTTCC TTAAAAAATG TGGTAGTAAG CAAGGTGAAG AAGATTACAG ACCACTATTT
    GAAAATTTTG TTCAAGACCT TCTTTCAACA GTCAATAAGC CTGAATGGCC AGCTGCTGAA
    CTACTCCTTA GTTTGTTAGG GAGACTGTTG GTTCATCAGT TCAGTAATAA GTCAACAGAG
    ATGGCTTTAA GAGTGGCATC TCTTGATTAC CTTGGCACTG TTGCTGCACG ACTGAGGAAA
    GATGCTGTTA CAAGCAAAAT GGATCAAGGG TCTATAGAAC GAATTTTAAA ACAGGTTTCA
    GGAGGGGAAG ATGAAATACA GCAATTGCAA AAAGCATTGC TTGATTACTT GGATGAAAAC
    ACGGAGACTG ATCCTTCACT GGTGTTTTCT CGTAAATTCT ATATTGCCCA GTGGTTCCGT
    GATACAACAC TGGAAACAGA AAAAGCAATG AAATCACAAA AAGATGAAGA ATCATCTGAA
    GGAGCACATC ATGCAAAGGA AGTTGAGACA ACTGGTCAAA TAATGCATCG AGCTGAAAAC
    CGGAAGAAGT TTCTTAGAAG CATTATCAAA ACCACTCCTT CTCAGTTCAG CACATTAAAA
    ATGAACTCTG ATACTGTGGA CTATGATGAT GCTTGTTTGA TTGTTCGATA CTTGGCCTCT
    ATGAGGCCGT TTGCCCAGAG CTTTGATATT TATTTGACAC AGATCCTTCG AGTCCTTGGT
    GAAAATGCAA TTGCCGTTCG AACAAAAGCC ATGAAGTGTT TATCTGAGGT TGTTGCTGTA
    GACCCCAGTA TTCTAGCAAG GCTGGATATG CAGCGAGGAG TTCATGGACG ATTAATGGAT
    AACTCCACTA GTGTCAGAGA AGCAGCAGTG GAATTACTAG GTCGATTTGT CCTTTGTCGA
    CCTCAGCTTG CTGAGCAGTA TTATGATATG CTGATTGAAA GAATATTGGA TACTGGTATC
    AGTGTCAGAA AAAGGGTGAT AAAGATTCTA AGAGACATCT GTATTGAACA ACCGACATTT
    CCAAAAATTA CAGAAATGTG TGTAAAAATG ATTCGCAGGG TAAATGATGA AGAGGGCATT
    AAGAAATTAG TAAATGAAAC ATTCCAAAAA CTCTGGTTTA CTCCAACTCC ACACAATGAC
    AAAGAAGCAA TGACAAGGAA GATTTTAAAT ATCACTGATG TGGTTGCAGC ATGCAGAGAT
    ACTGGATATG ACTGGTTTGA GCAGCTTCTT CAAAATTTGT TGAAGTCTGA AGAGGATTCC
    TCATATAAAC CTGTGAAGAA AGCTTGTACT CAACTTGTTG ATAACCTAGT TGAGCACATT
    CTTAAATATG AGGAATCTCT AGCTGACTCT GACAATAAAG GTGTGAATTC TGGAAGATTG
    GTAGCTTGCA TAACCACTTT GTTCTTATTC AGCAAAATAA GACCCCAGCT CATGGTTAAA
    CATGCCATGA CTATGCAGCC ATATCTTACC ACTAAATGTA GTACACAAAA TGATTTCATG
    GTTATCTGCA ATGTTGCAAA AATTCTAGAA CTAGTTGTAC CACTGATGGA ACATCCCAGT
    GAAACTTTCC TTGCCACTAT TGAAGAAGAT CTAATGAAGC TTATCATCAA ATATGGCATG
    ACTGTAGTGC AACATTGTGT AAGCTGTCTT GGGGCTGTTG TAAATAAAGT GACACAAAAT
    TTTAAATTTG TGTGGGCCTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT
    CAACACCAAG AGGACCCAAA TAACACTTCA CTGCTAACAA ACAAACCAGC ACTTCTGAGA
    TCCCTATTCA CTGTTGGGGC ACTGTGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA
    GGCAACAGTA AGGTTAACAT AAAGGATAAA GTCCTTGAAC TATTGATGTA TTTTACAAAG
    CATTCAGATG AAGAAGTGCA AACAAAAGCC ATCATTGGTC TAGGATTTGC CTTTATTCAG
    CACCCAAGTC TAATGTTTGA GCAAGAGGTG AAAAATCTAT ACAATAATAT TTTATCTGAT
    AAAAACTCCT CAGTAAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA
    GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAGGTTGC AAAACAGGAA
    GATTTGAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAC
    CTCAAACAGG TGCTTGAGGC GTTTTTTCAC ACCCAATCAA GTGTACGCCA CTTCGCCCTC
    AATGTCATTG CATTGACTCT AAACCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCGTAT
    TTAATTGCTA TGGGCACAGA CCCAGAACCA GCTATGCGGA ACAAGGCTGA TCAGCAGCTT
    GTGGAAATAG ATAAAAAATA TGCTGGATTC ATTCACATGA AAGCAGTGGC TGGCATGAAG
    ATGTCTTACC AGGTACAACA GGCAATCAAC ACATGCCTAA AAGATCCTGT AAGGGGTTTC
    CGGCAGGACG AGTCCTCCAG TGCTTTGTGT TCACACCTTT ACTCCATGAT CCGGGGAAAC
    CGCCAGCACA GACGCGCCTT TCTGATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA
    ACAGAAGTGA ATATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA
    CAGGAAGAGC CTTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCTGT TTCTGGTAGT
    AACCTACTGC AGTCATTCAA GGAGTCTATG GTAAAAGACA AAAGGAAAGA GAGAAAATCA
    TCTCCTGGTA AGGAAAATGA GTCAAGCGAG AGTGAAGAAG AAGTCTCTAG GCCTCGAAAG
    TCACGGAAAC GTGTAGATTC GGATTCAGAT TCGGATTCAG AAGATGATAT AAATTCGGTG
    ATGAAATGTT TGCCAGAAAA CTCAGCTCCT TTAATTGAGT TTGCAAATGT CTCCCAGGGT
    ATTTTATTGC TTCTGATGTT AAAACAACAT TTGAAGAATC TCTGTGGATT TTCTGATAGT
    AAAATTCAGA AATATTCTCC ATCCGAATCT GCAAAAGTGT ATGATAAAGC GATAAACCGG
    AAAACAGGAG TTCATTTTCA TCCCAAGCAA ACACTGGACT TCCTGCGGAG TGATATGGCT
    AATTCCAAAA TCACTGAAGA TGTGAAGAGG AGTATAGTAA AACAGTACCT AGATTTCAAA
    CTTCTCATGG AACATCTGGA CCCTGATGAA GAGGAAGAAG AAGGGGAGGT GTCAGCCAGC
    ACAAATGCTC GGAACAAAGC AATTACCTCG CTGCTTGGAG GAGGCAGCCC TAAAAATAAT
    GCAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA
    GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT
    GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGACCGACCA
    CAGATTGCAA GAGTAGTACA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC
    TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG
    GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT
    AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT
    TCCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006059620.1
    CDS327..7115
    Translation

    Target ORF information:

    RefSeq Version XM_006059558.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis Nipped-B homolog (Drosophila) (NIPBL), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006059558.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGTCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCTACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAACGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGGTAGT
    ATAGATCAAT CAGTGTTAAA AGAATTACCC CCCGAACTCC TGGCAGAAAT TGAATCCACC
    ATGCCACTTT GTGAACGTGT GAAAATGAAT AAACGCAAGC GTAGCACAGT CAATGAAAAA
    CCAAAATATG CTGAAATCAG TTCAGATGAA GATAATGATA GTGATGAAAC TTTTGAATCC
    TCTAGGAAAC GACATAAAAA AGATGATGAT AAAGCTTGGG AATATGAAGA GCGTGACAGA
    AGAAGCTCTG GGGATCATAG GAGAAGTGGC CACTCTCATG AAGGAAGAAG GAGTTCAGGT
    GGTGGTCGTT ATCGAAACCG AAGCCCATCA GATTCTGACA TGGAAGATTA TTCTCCTCCT
    CCCAGCCTTA GTGAGGTCGC TAGAAAAATG AAGAAAAAAG AAAAACAGAA GAAGCGGAAA
    GCTTATGAAC CAAAACTAAC ACCCGAAGAA ATGATGGATT CTTCAACTTT TAAGAGATTC
    ACAGCCTCAA TAGAGAATAT TTTGGATAAC TTAGAAGATA TGGATTTTAC TGCATTTGGT
    GATGATGATG AAATTCCTCA GGAACTGCTC TTAGGAAAAC ATCAGCTTAA TGAACTTGGC
    AGTGAATCTG CTAAAATTAA AGCAATGGGT ATAATGGATA AGCTTTCCAC TGACAAAACT
    GTGAAAGTCC TAAATATCTT GGAAAAGAAT ATTCAAGATG GGTCAAAGCT CTCCACTTTG
    TTAAATCATA ATAACGATAC TGAAGAAGAA GAAAGGTTAT GGAGAGACCT TATTATGGAG
    AGAGTGACAA AATCAGCAGA TGCCTGTCTT ACAACTATAA ACATCATGAC ATCCCCTAAC
    ATGCCAAAAG CTGTATATAT TGAGGATGTA ATCGAAAGAG TTATACAGTA CACTAAATTT
    CATTTGCAGA ATACACTTTA TCCTCAGTAT GATCCTGTTT ACAGATTAGA TCCTCATGGA
    GGAGGCTTAC TAAGTTCAAA GGCAAAACGG GCTAAATGTT CTACCCATAA GCAGAGAGTA
    ATAGTAATGC TTTATAATAA AGTTTGTGAC ATTGTTAGCA GCTTATCAGA ACTACTAGAA
    ATACAACTTC TAACAGACAC AACAATCCTT CAGGTATCTT CAATGGGAAT AACACCATTT
    TTTGTGGAAA ATGTCAGTGA ACTACAATTG TGTGCCATTA AATTAGTCAC TGCAGTATTC
    TCAAGATATG AAAAACATAG GCAGTTAATT TTGGAAGAAA TTTTTACTTC ACTTGCAAGA
    TTACCTACCA GCAAGAGGAG CTTAAGGAAC TTCAGGTTAA ATAGTAGTGA TATGGATGGA
    GAGCCTATGT ATATTCAGAT GGTTACAGCA CTGGTTTTAC AACTGATTCA GTGTGTAGTA
    CACTTACCAT CATCAGAGAA GGACTCTAAT TCAGAAGAGG ACTCAAATAA AAAGGTTGAC
    CAGGATGTTG TCATTACCAA TTCTTATGAA ACAGCCATGC GAACAGCCCA AAACTTCCTT
    TCCATCTTCC TTAAAAAATG TGGTAGTAAG CAAGGTGAAG AAGATTACAG ACCACTATTT
    GAAAATTTTG TTCAAGACCT TCTTTCAACA GTCAATAAGC CTGAATGGCC AGCTGCTGAA
    CTACTCCTTA GTTTGTTAGG GAGACTGTTG GTTCATCAGT TCAGTAATAA GTCAACAGAG
    ATGGCTTTAA GAGTGGCATC TCTTGATTAC CTTGGCACTG TTGCTGCACG ACTGAGGAAA
    GATGCTGTTA CAAGCAAAAT GGATCAAGGG TCTATAGAAC GAATTTTAAA ACAGGTTTCA
    GGAGGGGAAG ATGAAATACA GCAATTGCAA AAAGCATTGC TTGATTACTT GGATGAAAAC
    ACGGAGACTG ATCCTTCACT GGTGTTTTCT CGTAAATTCT ATATTGCCCA GTGGTTCCGT
    GATACAACAC TGGAAACAGA AAAAGCAATG AAATCACAAA AAGATGAAGA ATCATCTGAA
    GGAGCACATC ATGCAAAGGA AGTTGAGACA ACTGGTCAAA TAATGCATCG AGCTGAAAAC
    CGGAAGAAGT TTCTTAGAAG CATTATCAAA ACCACTCCTT CTCAGTTCAG CACATTAAAA
    ATGAACTCTG ATACTGTGGA CTATGATGAT GCTTGTTTGA TTGTTCGATA CTTGGCCTCT
    ATGAGGCCGT TTGCCCAGAG CTTTGATATT TATTTGACAC AGATCCTTCG AGTCCTTGGT
    GAAAATGCAA TTGCCGTTCG AACAAAAGCC ATGAAGTGTT TATCTGAGGT TGTTGCTGTA
    GACCCCAGTA TTCTAGCAAG GCTGGATATG CAGCGAGGAG TTCATGGACG ATTAATGGAT
    AACTCCACTA GTGTCAGAGA AGCAGCAGTG GAATTACTAG GTCGATTTGT CCTTTGTCGA
    CCTCAGCTTG CTGAGCAGTA TTATGATATG CTGATTGAAA GAATATTGGA TACTGGTATC
    AGTGTCAGAA AAAGGGTGAT AAAGATTCTA AGAGACATCT GTATTGAACA ACCGACATTT
    CCAAAAATTA CAGAAATGTG TGTAAAAATG ATTCGCAGGG TAAATGATGA AGAGGGCATT
    AAGAAATTAG TAAATGAAAC ATTCCAAAAA CTCTGGTTTA CTCCAACTCC ACACAATGAC
    AAAGAAGCAA TGACAAGGAA GATTTTAAAT ATCACTGATG TGGTTGCAGC ATGCAGAGAT
    ACTGGATATG ACTGGTTTGA GCAGCTTCTT CAAAATTTGT TGAAGTCTGA AGAGGATTCC
    TCATATAAAC CTGTGAAGAA AGCTTGTACT CAACTTGTTG ATAACCTAGT TGAGCACATT
    CTTAAATATG AGGAATCTCT AGCTGACTCT GACAATAAAG GTGTGAATTC TGGAAGATTG
    GTAGCTTGCA TAACCACTTT GTTCTTATTC AGCAAAATAA GACCCCAGCT CATGGTTAAA
    CATGCCATGA CTATGCAGCC ATATCTTACC ACTAAATGTA GTACACAAAA TGATTTCATG
    GTTATCTGCA ATGTTGCAAA AATTCTAGAA CTAGTTGTAC CACTGATGGA ACATCCCAGT
    GAAACTTTCC TTGCCACTAT TGAAGAAGAT CTAATGAAGC TTATCATCAA ATATGGCATG
    ACTGTAGTGC AACATTGTGT AAGCTGTCTT GGGGCTGTTG TAAATAAAGT GACACAAAAT
    TTTAAATTTG TGTGGGCCTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT
    CAACACCAAG AGGACCCAAA TAACACTTCA CTGCTAACAA ACAAACCAGC ACTTCTGAGA
    TCCCTATTCA CTGTTGGGGC ACTGTGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA
    GGCAACAGTA AGGTTAACAT AAAGGATAAA GTCCTTGAAC TATTGATGTA TTTTACAAAG
    CATTCAGATG AAGAAGTGCA AACAAAAGCC ATCATTGGTC TAGGATTTGC CTTTATTCAG
    CACCCAAGTC TAATGTTTGA GCAAGAGGTG AAAAATCTAT ACAATAATAT TTTATCTGAT
    AAAAACTCCT CAGTAAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA
    GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAGGTTGC AAAACAGGAA
    GATTTGAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAC
    CTCAAACAGG TGCTTGAGGC GTTTTTTCAC ACCCAATCAA GTGTACGCCA CTTCGCCCTC
    AATGTCATTG CATTGACTCT AAACCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCGTAT
    TTAATTGCTA TGGGCACAGA CCCAGAACCA GCTATGCGGA ACAAGGCTGA TCAGCAGCTT
    GTGGAAATAG ATAAAAAATA TGCTGGATTC ATTCACATGA AAGCAGTGGC TGGCATGAAG
    ATGTCTTACC AGGTACAACA GGCAATCAAC ACATGCCTAA AAGATCCTGT AAGGGGTTTC
    CGGCAGGACG AGTCCTCCAG TGCTTTGTGT TCACACCTTT ACTCCATGAT CCGGGGAAAC
    CGCCAGCACA GACGCGCCTT TCTGATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA
    ACAGAAGTGA ATATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA
    CAGGAAGAGC CTTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCTGT TTCTGGTAGT
    AACCTACTGC AGTCATTCAA GGAGTCTATG GTAAAAGACA AAAGGAAAGA GAGAAAATCA
    TCTCCTGGTA AGGAAAATGA GTCAAGCGAG AGTGAAGAAG AAGTCTCTAG GCCTCGAAAG
    TCACGGAAAC GTGTAGATTC GGATTCAGAT TCGGATTCAG AAGATGATAT AAATTCGGTG
    ATGAAATGTT TGCCAGAAAA CTCAGCTCCT TTAATTGAGT TTGCAAATGT CTCCCAGGGT
    ATTTTATTGC TTCTGATGTT AAAACAACAT TTGAAGAATC TCTGTGGATT TTCTGATAGT
    AAAATTCAGA AATATTCTCC ATCCGAATCT GCAAAAGTGT ATGATAAAGC GATAAACCGG
    AAAACAGGAG TTCATTTTCA TCCCAAGCAA ACACTGGACT TCCTGCGGAG TGATATGGCT
    AATTCCAAAA TCACTGAAGA TGTGAAGAGG AGTATAGTAA AACAGTACCT AGATTTCAAA
    CTTCTCATGG AACATCTGGA CCCTGATGAA GAGGAAGAAG AAGGGGAGGT GTCAGCCAGC
    ACAAATGCTC GGAACAAAGC AATTACCTCG CTGCTTGGAG GAGGCAGCCC TAAAAATAAT
    GCAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA
    GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT
    GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGACCGACCA
    CAGATTGCAA GAGTAGTACA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC
    TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG
    GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT
    AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT
    TCCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu53882
    Clone ID Related Accession (Same CDS sequence) XM_006059558.2
    Accession Version XM_006059558.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product nipped-B-like protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037563.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006059558.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGTCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCTACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAACGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGGTAGT
    ATAGATCAAT CAGTGTTAAA AGAATTACCC CCCGAACTCC TGGCAGAAAT TGAATCCACC
    ATGCCACTTT GTGAACGTGT GAAAATGAAT AAACGCAAGC GTAGCACAGT CAATGAAAAA
    CCAAAATATG CTGAAATCAG TTCAGATGAA GATAATGATA GTGATGAAAC TTTTGAATCC
    TCTAGGAAAC GACATAAAAA AGATGATGAT AAAGCTTGGG AATATGAAGA GCGTGACAGA
    AGAAGCTCTG GGGATCATAG GAGAAGTGGC CACTCTCATG AAGGAAGAAG GAGTTCAGGT
    GGTGGTCGTT ATCGAAACCG AAGCCCATCA GATTCTGACA TGGAAGATTA TTCTCCTCCT
    CCCAGCCTTA GTGAGGTCGC TAGAAAAATG AAGAAAAAAG AAAAACAGAA GAAGCGGAAA
    GCTTATGAAC CAAAACTAAC ACCCGAAGAA ATGATGGATT CTTCAACTTT TAAGAGATTC
    ACAGCCTCAA TAGAGAATAT TTTGGATAAC TTAGAAGATA TGGATTTTAC TGCATTTGGT
    GATGATGATG AAATTCCTCA GGAACTGCTC TTAGGAAAAC ATCAGCTTAA TGAACTTGGC
    AGTGAATCTG CTAAAATTAA AGCAATGGGT ATAATGGATA AGCTTTCCAC TGACAAAACT
    GTGAAAGTCC TAAATATCTT GGAAAAGAAT ATTCAAGATG GGTCAAAGCT CTCCACTTTG
    TTAAATCATA ATAACGATAC TGAAGAAGAA GAAAGGTTAT GGAGAGACCT TATTATGGAG
    AGAGTGACAA AATCAGCAGA TGCCTGTCTT ACAACTATAA ACATCATGAC ATCCCCTAAC
    ATGCCAAAAG CTGTATATAT TGAGGATGTA ATCGAAAGAG TTATACAGTA CACTAAATTT
    CATTTGCAGA ATACACTTTA TCCTCAGTAT GATCCTGTTT ACAGATTAGA TCCTCATGGA
    GGAGGCTTAC TAAGTTCAAA GGCAAAACGG GCTAAATGTT CTACCCATAA GCAGAGAGTA
    ATAGTAATGC TTTATAATAA AGTTTGTGAC ATTGTTAGCA GCTTATCAGA ACTACTAGAA
    ATACAACTTC TAACAGACAC AACAATCCTT CAGGTATCTT CAATGGGAAT AACACCATTT
    TTTGTGGAAA ATGTCAGTGA ACTACAATTG TGTGCCATTA AATTAGTCAC TGCAGTATTC
    TCAAGATATG AAAAACATAG GCAGTTAATT TTGGAAGAAA TTTTTACTTC ACTTGCAAGA
    TTACCTACCA GCAAGAGGAG CTTAAGGAAC TTCAGGTTAA ATAGTAGTGA TATGGATGGA
    GAGCCTATGT ATATTCAGAT GGTTACAGCA CTGGTTTTAC AACTGATTCA GTGTGTAGTA
    CACTTACCAT CATCAGAGAA GGACTCTAAT TCAGAAGAGG ACTCAAATAA AAAGGTTGAC
    CAGGATGTTG TCATTACCAA TTCTTATGAA ACAGCCATGC GAACAGCCCA AAACTTCCTT
    TCCATCTTCC TTAAAAAATG TGGTAGTAAG CAAGGTGAAG AAGATTACAG ACCACTATTT
    GAAAATTTTG TTCAAGACCT TCTTTCAACA GTCAATAAGC CTGAATGGCC AGCTGCTGAA
    CTACTCCTTA GTTTGTTAGG GAGACTGTTG GTTCATCAGT TCAGTAATAA GTCAACAGAG
    ATGGCTTTAA GAGTGGCATC TCTTGATTAC CTTGGCACTG TTGCTGCACG ACTGAGGAAA
    GATGCTGTTA CAAGCAAAAT GGATCAAGGG TCTATAGAAC GAATTTTAAA ACAGGTTTCA
    GGAGGGGAAG ATGAAATACA GCAATTGCAA AAAGCATTGC TTGATTACTT GGATGAAAAC
    ACGGAGACTG ATCCTTCACT GGTGTTTTCT CGTAAATTCT ATATTGCCCA GTGGTTCCGT
    GATACAACAC TGGAAACAGA AAAAGCAATG AAATCACAAA AAGATGAAGA ATCATCTGAA
    GGAGCACATC ATGCAAAGGA AGTTGAGACA ACTGGTCAAA TAATGCATCG AGCTGAAAAC
    CGGAAGAAGT TTCTTCGAAG CATTATCAAA ACCACTCCTT CTCAGTTCAG CACATTAAAA
    ATGAACTCTG ATACTGTGGA CTATGATGAT GCTTGTTTGA TTGTTCGATA CTTGGCCTCT
    ATGAGGCCGT TTGCCCAGAG CTTTGATATT TATTTGACAC AGATCCTTCG AGTCCTTGGT
    GAAAATGCAA TTGCCGTTCG AACAAAAGCC ATGAAGTGTT TATCTGAGGT TGTTGCTGTA
    GACCCCAGTA TTCTAGCAAG GCTGGATATG CAGCGAGGAG TTCATGGACG ATTAATGGAT
    AACTCCACTA GTGTCAGAGA AGCAGCAGTG GAATTACTAG GTCGATTTGT CCTTTGTCGA
    CCTCAGCTTG CTGAGCAGTA TTATGATATG CTGATTGAAA GAATATTGGA TACTGGTATC
    AGTGTCAGAA AAAGGGTGAT AAAGATTCTA AGAGACATCT GTATTGAACA ACCGACATTT
    CCAAAAATTA CAGAAATGTG TGTAAAAATG ATTCGCAGGG TAAATGATGA AGAGGGCATT
    AAGAAATTAG TAAATGAAAC ATTCCAAAAA CTCTGGTTTA CTCCAACTCC ACACAATGAC
    AAAGAAGCAA TGACAAGGAA GATTTTAAAT ATCACTGATG TGGTTGCAGC ATGCAGAGAT
    ACTGGATATG ACTGGTTTGA GCAGCTTCTT CAAAATTTGT TGAAGTCTGA AGAGGATTCC
    TCATATAAAC CTGTGAAGAA AGCTTGTACT CAACTTGTTG ATAACCTAGT TGAGCACATT
    CTTAAATATG AGGAATCTCT AGCTGACTCT GACAATAAAG GTGTGAATTC TGGAAGATTG
    GTAGCTTGCA TAACCACTTT GTTCTTATTC AGCAAAATAA GACCCCAGCT CATGGTTAAA
    CATGCCATGA CTATGCAGCC ATATCTTACC ACTAAATGTA GTACACAAAA TGATTTCATG
    GTTATCTGCA ATGTTGCAAA AATTCTAGAA CTAGTTGTAC CACTGATGGA ACATCCCAGT
    GAAACTTTCC TTGCCACTAT TGAAGAAGAT CTAATGAAGC TTATCATCAA ATATGGCATG
    ACTGTAGTGC AACATTGTGT AAGCTGTCTT GGGGCTGTTG TAAATAAAGT GACACAAAAT
    TTTAAATTTG TGTGGGCCTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT
    CAACACCAAG AGGACCCAAA TAACACTTCA CTGCTAACAA ACAAACCAGC ACTTCTGAGA
    TCCCTATTCA CTGTTGGGGC ACTGTGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA
    GGCAACAGTA AGGTTAACAT AAAGGATAAA GTCCTTGAAC TATTGATGTA TTTTACAAAG
    CATTCAGATG AAGAAGTGCA AACAAAAGCC ATCATTGGTC TAGGATTTGC CTTTATTCAG
    CACCCAAGTC TAATGTTTGA GCAAGAGGTG AAAAATCTAT ACAATAATAT TTTATCTGAT
    AAAAACTCCT CAGTAAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA
    GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAGGTTGC AAAACAGGAA
    GATTTGAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAC
    CTCAAACAGG TGCTTGAGGC GTTTTTTCAC ACCCAATCAA GTGTACGCCA CTTCGCCCTC
    AATGTCATTG CATTGACTCT AAACCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCGTAT
    TTAATTGCTA TGGGCACAGA CCCAGAACCA GCTATGCGGA ACAAGGCTGA TCAGCAGCTT
    GTGGAAATAG ATAAAAAATA TGCTGGATTC ATTCACATGA AAGCAGTGGC TGGCATGAAG
    ATGTCTTACC AGGTACAACA GGCAATCAAC ACATGCCTAA AAGATCCTGT AAGGGGTTTC
    CGGCAGGACG AGTCCTCCAG TGCTTTGTGT TCACACCTTT ACTCCATGAT CCGGGGAAAC
    CGCCAGCACA GACGCGCCTT TCTGATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA
    ACAGAAGTGA ATATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA
    CAGGAAGAGC CTTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCTGT TTCTGGTAGT
    AACCTACTGC AGTCATTCAA GGAGTCTATG GTAAAAGACA AAAGGAAAGA GAGAAAATCA
    TCTCCTGGTA AGGAAAATGA GTCAAGCGAG AGTGAAGAAG AAGTCTCTAG GCCTCGAAAG
    TCACGGAAAC GTGTAGATTC GGATTCAGAT TCGGATTCAG AAGATGATAT AAATTCGGTG
    ATGAAATGTT TGCCAGAAAA CTCAGCTCCT TTAATTGAGT TTGCAAATGT CTCCCAGGGT
    ATTTTATTGC TTCTGATGTT AAAACAACAT TTGAAGAATC TCTGTGGATT TTCTGATAGT
    AAAATTCAGA AATATTCTCC ATCCGAATCT GCAAAAGTGT ATGATAAAGC GATAAACCGG
    AAAACAGGAG TTCATTTTCA TCCCAAGCAA ACACTGGACT TCCTGCGGAG TGATATGGCT
    AATTCCAAAA TCACTGAAGA TGTGAAGAGG AGTATAGTAA AACAGTACCT AGATTTCAAA
    CTTCTCATGG AACATCTGGA CCCTGATGAA GAGGAAGAAG AAGGGGAGGT GTCAGCCAGC
    ACAAATGCTC GGAACAAAGC AATTACCTCG CTGCTTGGAG GAGGCAGCCC TAAAAATAAT
    GCAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA
    GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT
    GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGACCGACCA
    CAGATTGCAA GAGTAGTACA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC
    TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG
    GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT
    AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT
    TCCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006059620.1
    CDS209..6997
    Translation

    Target ORF information:

    RefSeq Version XM_006059558.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis NIPBL, cohesin loading factor (NIPBL), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006059558.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGTCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCTACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAACGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGGTAGT
    ATAGATCAAT CAGTGTTAAA AGAATTACCC CCCGAACTCC TGGCAGAAAT TGAATCCACC
    ATGCCACTTT GTGAACGTGT GAAAATGAAT AAACGCAAGC GTAGCACAGT CAATGAAAAA
    CCAAAATATG CTGAAATCAG TTCAGATGAA GATAATGATA GTGATGAAAC TTTTGAATCC
    TCTAGGAAAC GACATAAAAA AGATGATGAT AAAGCTTGGG AATATGAAGA GCGTGACAGA
    AGAAGCTCTG GGGATCATAG GAGAAGTGGC CACTCTCATG AAGGAAGAAG GAGTTCAGGT
    GGTGGTCGTT ATCGAAACCG AAGCCCATCA GATTCTGACA TGGAAGATTA TTCTCCTCCT
    CCCAGCCTTA GTGAGGTCGC TAGAAAAATG AAGAAAAAAG AAAAACAGAA GAAGCGGAAA
    GCTTATGAAC CAAAACTAAC ACCCGAAGAA ATGATGGATT CTTCAACTTT TAAGAGATTC
    ACAGCCTCAA TAGAGAATAT TTTGGATAAC TTAGAAGATA TGGATTTTAC TGCATTTGGT
    GATGATGATG AAATTCCTCA GGAACTGCTC TTAGGAAAAC ATCAGCTTAA TGAACTTGGC
    AGTGAATCTG CTAAAATTAA AGCAATGGGT ATAATGGATA AGCTTTCCAC TGACAAAACT
    GTGAAAGTCC TAAATATCTT GGAAAAGAAT ATTCAAGATG GGTCAAAGCT CTCCACTTTG
    TTAAATCATA ATAACGATAC TGAAGAAGAA GAAAGGTTAT GGAGAGACCT TATTATGGAG
    AGAGTGACAA AATCAGCAGA TGCCTGTCTT ACAACTATAA ACATCATGAC ATCCCCTAAC
    ATGCCAAAAG CTGTATATAT TGAGGATGTA ATCGAAAGAG TTATACAGTA CACTAAATTT
    CATTTGCAGA ATACACTTTA TCCTCAGTAT GATCCTGTTT ACAGATTAGA TCCTCATGGA
    GGAGGCTTAC TAAGTTCAAA GGCAAAACGG GCTAAATGTT CTACCCATAA GCAGAGAGTA
    ATAGTAATGC TTTATAATAA AGTTTGTGAC ATTGTTAGCA GCTTATCAGA ACTACTAGAA
    ATACAACTTC TAACAGACAC AACAATCCTT CAGGTATCTT CAATGGGAAT AACACCATTT
    TTTGTGGAAA ATGTCAGTGA ACTACAATTG TGTGCCATTA AATTAGTCAC TGCAGTATTC
    TCAAGATATG AAAAACATAG GCAGTTAATT TTGGAAGAAA TTTTTACTTC ACTTGCAAGA
    TTACCTACCA GCAAGAGGAG CTTAAGGAAC TTCAGGTTAA ATAGTAGTGA TATGGATGGA
    GAGCCTATGT ATATTCAGAT GGTTACAGCA CTGGTTTTAC AACTGATTCA GTGTGTAGTA
    CACTTACCAT CATCAGAGAA GGACTCTAAT TCAGAAGAGG ACTCAAATAA AAAGGTTGAC
    CAGGATGTTG TCATTACCAA TTCTTATGAA ACAGCCATGC GAACAGCCCA AAACTTCCTT
    TCCATCTTCC TTAAAAAATG TGGTAGTAAG CAAGGTGAAG AAGATTACAG ACCACTATTT
    GAAAATTTTG TTCAAGACCT TCTTTCAACA GTCAATAAGC CTGAATGGCC AGCTGCTGAA
    CTACTCCTTA GTTTGTTAGG GAGACTGTTG GTTCATCAGT TCAGTAATAA GTCAACAGAG
    ATGGCTTTAA GAGTGGCATC TCTTGATTAC CTTGGCACTG TTGCTGCACG ACTGAGGAAA
    GATGCTGTTA CAAGCAAAAT GGATCAAGGG TCTATAGAAC GAATTTTAAA ACAGGTTTCA
    GGAGGGGAAG ATGAAATACA GCAATTGCAA AAAGCATTGC TTGATTACTT GGATGAAAAC
    ACGGAGACTG ATCCTTCACT GGTGTTTTCT CGTAAATTCT ATATTGCCCA GTGGTTCCGT
    GATACAACAC TGGAAACAGA AAAAGCAATG AAATCACAAA AAGATGAAGA ATCATCTGAA
    GGAGCACATC ATGCAAAGGA AGTTGAGACA ACTGGTCAAA TAATGCATCG AGCTGAAAAC
    CGGAAGAAGT TTCTTCGAAG CATTATCAAA ACCACTCCTT CTCAGTTCAG CACATTAAAA
    ATGAACTCTG ATACTGTGGA CTATGATGAT GCTTGTTTGA TTGTTCGATA CTTGGCCTCT
    ATGAGGCCGT TTGCCCAGAG CTTTGATATT TATTTGACAC AGATCCTTCG AGTCCTTGGT
    GAAAATGCAA TTGCCGTTCG AACAAAAGCC ATGAAGTGTT TATCTGAGGT TGTTGCTGTA
    GACCCCAGTA TTCTAGCAAG GCTGGATATG CAGCGAGGAG TTCATGGACG ATTAATGGAT
    AACTCCACTA GTGTCAGAGA AGCAGCAGTG GAATTACTAG GTCGATTTGT CCTTTGTCGA
    CCTCAGCTTG CTGAGCAGTA TTATGATATG CTGATTGAAA GAATATTGGA TACTGGTATC
    AGTGTCAGAA AAAGGGTGAT AAAGATTCTA AGAGACATCT GTATTGAACA ACCGACATTT
    CCAAAAATTA CAGAAATGTG TGTAAAAATG ATTCGCAGGG TAAATGATGA AGAGGGCATT
    AAGAAATTAG TAAATGAAAC ATTCCAAAAA CTCTGGTTTA CTCCAACTCC ACACAATGAC
    AAAGAAGCAA TGACAAGGAA GATTTTAAAT ATCACTGATG TGGTTGCAGC ATGCAGAGAT
    ACTGGATATG ACTGGTTTGA GCAGCTTCTT CAAAATTTGT TGAAGTCTGA AGAGGATTCC
    TCATATAAAC CTGTGAAGAA AGCTTGTACT CAACTTGTTG ATAACCTAGT TGAGCACATT
    CTTAAATATG AGGAATCTCT AGCTGACTCT GACAATAAAG GTGTGAATTC TGGAAGATTG
    GTAGCTTGCA TAACCACTTT GTTCTTATTC AGCAAAATAA GACCCCAGCT CATGGTTAAA
    CATGCCATGA CTATGCAGCC ATATCTTACC ACTAAATGTA GTACACAAAA TGATTTCATG
    GTTATCTGCA ATGTTGCAAA AATTCTAGAA CTAGTTGTAC CACTGATGGA ACATCCCAGT
    GAAACTTTCC TTGCCACTAT TGAAGAAGAT CTAATGAAGC TTATCATCAA ATATGGCATG
    ACTGTAGTGC AACATTGTGT AAGCTGTCTT GGGGCTGTTG TAAATAAAGT GACACAAAAT
    TTTAAATTTG TGTGGGCCTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT
    CAACACCAAG AGGACCCAAA TAACACTTCA CTGCTAACAA ACAAACCAGC ACTTCTGAGA
    TCCCTATTCA CTGTTGGGGC ACTGTGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA
    GGCAACAGTA AGGTTAACAT AAAGGATAAA GTCCTTGAAC TATTGATGTA TTTTACAAAG
    CATTCAGATG AAGAAGTGCA AACAAAAGCC ATCATTGGTC TAGGATTTGC CTTTATTCAG
    CACCCAAGTC TAATGTTTGA GCAAGAGGTG AAAAATCTAT ACAATAATAT TTTATCTGAT
    AAAAACTCCT CAGTAAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA
    GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAGGTTGC AAAACAGGAA
    GATTTGAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAC
    CTCAAACAGG TGCTTGAGGC GTTTTTTCAC ACCCAATCAA GTGTACGCCA CTTCGCCCTC
    AATGTCATTG CATTGACTCT AAACCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCGTAT
    TTAATTGCTA TGGGCACAGA CCCAGAACCA GCTATGCGGA ACAAGGCTGA TCAGCAGCTT
    GTGGAAATAG ATAAAAAATA TGCTGGATTC ATTCACATGA AAGCAGTGGC TGGCATGAAG
    ATGTCTTACC AGGTACAACA GGCAATCAAC ACATGCCTAA AAGATCCTGT AAGGGGTTTC
    CGGCAGGACG AGTCCTCCAG TGCTTTGTGT TCACACCTTT ACTCCATGAT CCGGGGAAAC
    CGCCAGCACA GACGCGCCTT TCTGATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA
    ACAGAAGTGA ATATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA
    CAGGAAGAGC CTTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCTGT TTCTGGTAGT
    AACCTACTGC AGTCATTCAA GGAGTCTATG GTAAAAGACA AAAGGAAAGA GAGAAAATCA
    TCTCCTGGTA AGGAAAATGA GTCAAGCGAG AGTGAAGAAG AAGTCTCTAG GCCTCGAAAG
    TCACGGAAAC GTGTAGATTC GGATTCAGAT TCGGATTCAG AAGATGATAT AAATTCGGTG
    ATGAAATGTT TGCCAGAAAA CTCAGCTCCT TTAATTGAGT TTGCAAATGT CTCCCAGGGT
    ATTTTATTGC TTCTGATGTT AAAACAACAT TTGAAGAATC TCTGTGGATT TTCTGATAGT
    AAAATTCAGA AATATTCTCC ATCCGAATCT GCAAAAGTGT ATGATAAAGC GATAAACCGG
    AAAACAGGAG TTCATTTTCA TCCCAAGCAA ACACTGGACT TCCTGCGGAG TGATATGGCT
    AATTCCAAAA TCACTGAAGA TGTGAAGAGG AGTATAGTAA AACAGTACCT AGATTTCAAA
    CTTCTCATGG AACATCTGGA CCCTGATGAA GAGGAAGAAG AAGGGGAGGT GTCAGCCAGC
    ACAAATGCTC GGAACAAAGC AATTACCTCG CTGCTTGGAG GAGGCAGCCC TAAAAATAAT
    GCAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA
    GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT
    GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGACCGACCA
    CAGATTGCAA GAGTAGTACA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC
    TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG
    GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT
    AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT
    TCCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu16136
    Clone ID Related Accession (Same CDS sequence) XM_006059556.1
    Accession Version XM_006059556.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product nipped-B-like protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784928.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGTCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCTACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAACGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGACGCACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACGCCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGATTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAC CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAGGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAATGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGATAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATAAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCGCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA ATCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCCG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTCAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
    GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
    TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT
    CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG
    CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG
    AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA
    TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
    CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC
    AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC
    ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
    ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC
    CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT
    AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
    CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
    AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA
    CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA
    CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTCACTGCA
    GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
    GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG
    GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT GATTCAGTGT
    GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAG
    GTTGACCAGG ATGTTGTCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC
    TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
    CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
    GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA
    ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GCACTGTTGC TGCACGACTG
    AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG
    GTTTCAGGAG GGGAAGATGA AATACAGCAA TTGCAAAAAG CATTGCTTGA TTACTTGGAT
    GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG
    TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
    TCTGAAGGAG CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATAAT GCATCGAGCT
    GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CTCCTTCTCA GTTCAGCACA
    TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG
    GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC
    CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT
    GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA
    ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT
    TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
    GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG
    ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG
    GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC
    AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC
    AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG
    GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
    CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
    AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
    GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT
    TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT
    CCCAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT
    GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA
    CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
    AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT
    CTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT
    TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTCC TTGAACTATT GATGTATTTT
    ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT
    ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA
    TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
    CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA
    CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
    CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC
    GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
    CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG
    CAGCTTGTGG AAATAGATAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC
    ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
    GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGG
    GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA
    GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
    CAGACACAGG AAGAGCCTTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT
    GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AAGACAAAAG GAAAGAGAGA
    AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT
    CGAAAGTCAC GGAAACGTGT AGATTCGGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT
    TCGGTGATGA AATGTTTGCC AGAAAACTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC
    CAGGGTATTT TATTGCTTCT GATGTTAAAA CAACATTTGA AGAATCTCTG TGGATTTTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA
    AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT
    ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT
    TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA
    GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
    ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG
    ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAC
    CGACCACAGA TTGCAAGAGT AGTACAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG
    GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG
    CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT
    GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT
    GGGACTTCCA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006059618.1
    CDS327..8741
    Translation

    Target ORF information:

    RefSeq Version XM_006059556.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis Nipped-B homolog (Drosophila) (NIPBL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006059556.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGTCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCTACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAACGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGACGCACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACGCCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGATTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAC CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAGGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAATGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGATAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATAAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCGCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA ATCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCCG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTCAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
    GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
    TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT
    CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG
    CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG
    AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA
    TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
    CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC
    AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC
    ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
    ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC
    CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT
    AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
    CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
    AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA
    CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA
    CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTCACTGCA
    GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
    GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG
    GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT GATTCAGTGT
    GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAG
    GTTGACCAGG ATGTTGTCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC
    TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
    CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
    GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA
    ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GCACTGTTGC TGCACGACTG
    AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG
    GTTTCAGGAG GGGAAGATGA AATACAGCAA TTGCAAAAAG CATTGCTTGA TTACTTGGAT
    GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG
    TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
    TCTGAAGGAG CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATAAT GCATCGAGCT
    GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CTCCTTCTCA GTTCAGCACA
    TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG
    GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC
    CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT
    GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA
    ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT
    TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
    GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG
    ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG
    GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC
    AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC
    AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG
    GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
    CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
    AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
    GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT
    TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT
    CCCAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT
    GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA
    CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
    AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT
    CTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT
    TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTCC TTGAACTATT GATGTATTTT
    ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT
    ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA
    TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
    CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA
    CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
    CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC
    GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
    CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG
    CAGCTTGTGG AAATAGATAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC
    ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
    GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGG
    GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA
    GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
    CAGACACAGG AAGAGCCTTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT
    GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AAGACAAAAG GAAAGAGAGA
    AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT
    CGAAAGTCAC GGAAACGTGT AGATTCGGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT
    TCGGTGATGA AATGTTTGCC AGAAAACTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC
    CAGGGTATTT TATTGCTTCT GATGTTAAAA CAACATTTGA AGAATCTCTG TGGATTTTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA
    AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT
    ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT
    TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA
    GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
    ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG
    ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAC
    CGACCACAGA TTGCAAGAGT AGTACAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG
    GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG
    CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT
    GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT
    GGGACTTCCA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu16137
    Clone ID Related Accession (Same CDS sequence) XM_006059557.1
    Accession Version XM_006059557.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8097bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product nipped-B-like protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784928.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGTCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCTACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAACGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGACGCACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACGCCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGATTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAC CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAGGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAATGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGATAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATAAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCGCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA ATCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCCG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTCAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
    GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
    TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT
    CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG
    CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG
    AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA
    TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
    CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC
    AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC
    ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
    ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC
    CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT
    AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
    CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
    AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA
    CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA
    CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTCACTGCA
    GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
    GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG
    GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT GATTCAGTGT
    GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAG
    GTTGACCAGG ATGTTGTCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC
    TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
    CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
    GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA
    ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GCACTGTTGC TGCACGACTG
    AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG
    GTTTCAGGAG GGGAAGATGA AATACAGCAA TTGCAAAAAG CATTGCTTGA TTACTTGGAT
    GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG
    TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
    TCTGAAGGAG CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATAAT GCATCGAGCT
    GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CTCCTTCTCA GTTCAGCACA
    TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG
    GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC
    CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT
    GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA
    ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT
    TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
    GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG
    ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG
    GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC
    AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC
    AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG
    GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
    CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
    AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
    GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT
    TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT
    CCCAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT
    GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA
    CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
    AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT
    CTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT
    TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTCC TTGAACTATT GATGTATTTT
    ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT
    ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA
    TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
    CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA
    CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
    CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC
    GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
    CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG
    CAGCTTGTGG AAATAGATAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC
    ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
    GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGG
    GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA
    GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
    CAGACACAGG AAGAGCCTTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT
    GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AAGACAAAAG GAAAGAGAGA
    AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT
    CGAAAGTCAC GGAAACGTGT AGATTCGGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT
    TCGGTGATGA AATGTTTGCC AGAAAACTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC
    CAGGGTATTT TATTGCTTCT GATGTTAAAA CAACATTTGA AGAATCTCTG TGGATTTTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA
    AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT
    ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT
    TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA
    GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
    ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC TCATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006059619.1
    CDS327..8423
    Translation

    Target ORF information:

    RefSeq Version XM_006059557.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis Nipped-B homolog (Drosophila) (NIPBL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006059557.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGTCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCTACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAACGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGACGCACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACGCCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGATTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAC CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAGGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAATGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGATAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATAAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCGCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA ATCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCCG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTCAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
    GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
    TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT
    CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG
    CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG
    AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA
    TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
    CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC
    AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC
    ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
    ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC
    CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT
    AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
    CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
    AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA
    CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA
    CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTCACTGCA
    GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
    GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG
    GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT GATTCAGTGT
    GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAG
    GTTGACCAGG ATGTTGTCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC
    TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
    CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
    GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA
    ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GCACTGTTGC TGCACGACTG
    AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG
    GTTTCAGGAG GGGAAGATGA AATACAGCAA TTGCAAAAAG CATTGCTTGA TTACTTGGAT
    GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG
    TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
    TCTGAAGGAG CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATAAT GCATCGAGCT
    GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CTCCTTCTCA GTTCAGCACA
    TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG
    GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC
    CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT
    GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA
    ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT
    TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
    GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG
    ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG
    GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC
    AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC
    AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG
    GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
    CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
    AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
    GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT
    TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT
    CCCAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT
    GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA
    CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
    AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT
    CTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT
    TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTCC TTGAACTATT GATGTATTTT
    ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT
    ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA
    TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
    CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA
    CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
    CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC
    GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
    CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG
    CAGCTTGTGG AAATAGATAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC
    ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
    GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGG
    GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA
    GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
    CAGACACAGG AAGAGCCTTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT
    GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AAGACAAAAG GAAAGAGAGA
    AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT
    CGAAAGTCAC GGAAACGTGT AGATTCGGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT
    TCGGTGATGA AATGTTTGCC AGAAAACTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC
    CAGGGTATTT TATTGCTTCT GATGTTAAAA CAACATTTGA AGAATCTCTG TGGATTTTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA
    AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT
    ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT
    TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA
    GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
    ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC TCATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu53879
    Clone ID Related Accession (Same CDS sequence) XM_006059556.2
    Accession Version XM_006059556.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product nipped-B-like protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037563.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006059556.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGTCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCTACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAACGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGACGCACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACGCCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGATTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAC CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAGGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAATGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGATAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATAAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCGCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA ATCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCCG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTCAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
    GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
    TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT
    CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG
    CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG
    AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA
    TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
    CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC
    AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC
    ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
    ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC
    CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT
    AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
    CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
    AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA
    CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA
    CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTCACTGCA
    GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
    GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG
    GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT GATTCAGTGT
    GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAG
    GTTGACCAGG ATGTTGTCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC
    TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
    CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
    GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA
    ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GCACTGTTGC TGCACGACTG
    AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG
    GTTTCAGGAG GGGAAGATGA AATACAGCAA TTGCAAAAAG CATTGCTTGA TTACTTGGAT
    GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG
    TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
    TCTGAAGGAG CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATAAT GCATCGAGCT
    GAAAACCGGA AGAAGTTTCT TCGAAGCATT ATCAAAACCA CTCCTTCTCA GTTCAGCACA
    TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG
    GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC
    CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT
    GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA
    ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT
    TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
    GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG
    ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG
    GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC
    AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC
    AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG
    GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
    CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
    AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
    GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT
    TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT
    CCCAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT
    GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA
    CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
    AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT
    CTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT
    TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTCC TTGAACTATT GATGTATTTT
    ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT
    ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA
    TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
    CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA
    CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
    CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC
    GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
    CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG
    CAGCTTGTGG AAATAGATAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC
    ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
    GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGG
    GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA
    GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
    CAGACACAGG AAGAGCCTTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT
    GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AAGACAAAAG GAAAGAGAGA
    AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT
    CGAAAGTCAC GGAAACGTGT AGATTCGGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT
    TCGGTGATGA AATGTTTGCC AGAAAACTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC
    CAGGGTATTT TATTGCTTCT GATGTTAAAA CAACATTTGA AGAATCTCTG TGGATTTTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA
    AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT
    ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT
    TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA
    GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
    ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG
    ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAC
    CGACCACAGA TTGCAAGAGT AGTACAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG
    GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG
    CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT
    GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT
    GGGACTTCCA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006059618.1
    CDS209..8623
    Translation

    Target ORF information:

    RefSeq Version XM_006059556.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis NIPBL, cohesin loading factor (NIPBL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006059556.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGTCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCTACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAACGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGACGCACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACGCCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGATTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAC CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAGGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAATGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGATAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATAAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCGCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA ATCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCCG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTCAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
    GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
    TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT
    CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG
    CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG
    AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA
    TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
    CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC
    AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC
    ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
    ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC
    CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT
    AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
    CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
    AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA
    CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA
    CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTCACTGCA
    GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
    GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG
    GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT GATTCAGTGT
    GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAG
    GTTGACCAGG ATGTTGTCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC
    TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
    CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
    GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA
    ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GCACTGTTGC TGCACGACTG
    AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG
    GTTTCAGGAG GGGAAGATGA AATACAGCAA TTGCAAAAAG CATTGCTTGA TTACTTGGAT
    GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG
    TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
    TCTGAAGGAG CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATAAT GCATCGAGCT
    GAAAACCGGA AGAAGTTTCT TCGAAGCATT ATCAAAACCA CTCCTTCTCA GTTCAGCACA
    TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG
    GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC
    CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT
    GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA
    ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT
    TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
    GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG
    ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG
    GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC
    AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC
    AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG
    GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
    CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
    AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
    GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT
    TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT
    CCCAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT
    GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA
    CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
    AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT
    CTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT
    TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTCC TTGAACTATT GATGTATTTT
    ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT
    ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA
    TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
    CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA
    CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
    CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC
    GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
    CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG
    CAGCTTGTGG AAATAGATAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC
    ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
    GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGG
    GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA
    GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
    CAGACACAGG AAGAGCCTTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT
    GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AAGACAAAAG GAAAGAGAGA
    AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT
    CGAAAGTCAC GGAAACGTGT AGATTCGGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT
    TCGGTGATGA AATGTTTGCC AGAAAACTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC
    CAGGGTATTT TATTGCTTCT GATGTTAAAA CAACATTTGA AGAATCTCTG TGGATTTTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA
    AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT
    ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT
    TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA
    GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
    ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG
    ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAC
    CGACCACAGA TTGCAAGAGT AGTACAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG
    GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG
    CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT
    GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT
    GGGACTTCCA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu53880
    Clone ID Related Accession (Same CDS sequence) XM_025270602.1
    Accession Version XM_025270602.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product nipped-B-like protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037563.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGTCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCTACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAACGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGACGCACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACGCCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGATTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAC CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAGGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAATGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGATAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATAAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCGCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA ATCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCCG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTCAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATTCGCTA GAAAAATGAA GAAAAAAGAA AAACAGAAGA AGCGGAAAGC TTATGAACCA
    AAACTAACAC CCGAAGAAAT GATGGATTCT TCAACTTTTA AGAGATTCAC AGCCTCAATA
    GAGAATATTT TGGATAACTT AGAAGATATG GATTTTACTG CATTTGGTGA TGATGATGAA
    ATTCCTCAGG AACTGCTCTT AGGAAAACAT CAGCTTAATG AACTTGGCAG TGAATCTGCT
    AAAATTAAAG CAATGGGTAT AATGGATAAG CTTTCCACTG ACAAAACTGT GAAAGTCCTA
    AATATCTTGG AAAAGAATAT TCAAGATGGG TCAAAGCTCT CCACTTTGTT AAATCATAAT
    AACGATACTG AAGAAGAAGA AAGGTTATGG AGAGACCTTA TTATGGAGAG AGTGACAAAA
    TCAGCAGATG CCTGTCTTAC AACTATAAAC ATCATGACAT CCCCTAACAT GCCAAAAGCT
    GTATATATTG AGGATGTAAT CGAAAGAGTT ATACAGTACA CTAAATTTCA TTTGCAGAAT
    ACACTTTATC CTCAGTATGA TCCTGTTTAC AGATTAGATC CTCATGGAGG AGGCTTACTA
    AGTTCAAAGG CAAAACGGGC TAAATGTTCT ACCCATAAGC AGAGAGTAAT AGTAATGCTT
    TATAATAAAG TTTGTGACAT TGTTAGCAGC TTATCAGAAC TACTAGAAAT ACAACTTCTA
    ACAGACACAA CAATCCTTCA GGTATCTTCA ATGGGAATAA CACCATTTTT TGTGGAAAAT
    GTCAGTGAAC TACAATTGTG TGCCATTAAA TTAGTCACTG CAGTATTCTC AAGATATGAA
    AAACATAGGC AGTTAATTTT GGAAGAAATT TTTACTTCAC TTGCAAGATT ACCTACCAGC
    AAGAGGAGCT TAAGGAACTT CAGGTTAAAT AGTAGTGATA TGGATGGAGA GCCTATGTAT
    ATTCAGATGG TTACAGCACT GGTTTTACAA CTGATTCAGT GTGTAGTACA CTTACCATCA
    TCAGAGAAGG ACTCTAATTC AGAAGAGGAC TCAAATAAAA AGGTTGACCA GGATGTTGTC
    ATTACCAATT CTTATGAAAC AGCCATGCGA ACAGCCCAAA ACTTCCTTTC CATCTTCCTT
    AAAAAATGTG GTAGTAAGCA AGGTGAAGAA GATTACAGAC CACTATTTGA AAATTTTGTT
    CAAGACCTTC TTTCAACAGT CAATAAGCCT GAATGGCCAG CTGCTGAACT ACTCCTTAGT
    TTGTTAGGGA GACTGTTGGT TCATCAGTTC AGTAATAAGT CAACAGAGAT GGCTTTAAGA
    GTGGCATCTC TTGATTACCT TGGCACTGTT GCTGCACGAC TGAGGAAAGA TGCTGTTACA
    AGCAAAATGG ATCAAGGGTC TATAGAACGA ATTTTAAAAC AGGTTTCAGG AGGGGAAGAT
    GAAATACAGC AATTGCAAAA AGCATTGCTT GATTACTTGG ATGAAAACAC GGAGACTGAT
    CCTTCACTGG TGTTTTCTCG TAAATTCTAT ATTGCCCAGT GGTTCCGTGA TACAACACTG
    GAAACAGAAA AAGCAATGAA ATCACAAAAA GATGAAGAAT CATCTGAAGG AGCACATCAT
    GCAAAGGAAG TTGAGACAAC TGGTCAAATA ATGCATCGAG CTGAAAACCG GAAGAAGTTT
    CTTCGAAGCA TTATCAAAAC CACTCCTTCT CAGTTCAGCA CATTAAAAAT GAACTCTGAT
    ACTGTGGACT ATGATGATGC TTGTTTGATT GTTCGATACT TGGCCTCTAT GAGGCCGTTT
    GCCCAGAGCT TTGATATTTA TTTGACACAG ATCCTTCGAG TCCTTGGTGA AAATGCAATT
    GCCGTTCGAA CAAAAGCCAT GAAGTGTTTA TCTGAGGTTG TTGCTGTAGA CCCCAGTATT
    CTAGCAAGGC TGGATATGCA GCGAGGAGTT CATGGACGAT TAATGGATAA CTCCACTAGT
    GTCAGAGAAG CAGCAGTGGA ATTACTAGGT CGATTTGTCC TTTGTCGACC TCAGCTTGCT
    GAGCAGTATT ATGATATGCT GATTGAAAGA ATATTGGATA CTGGTATCAG TGTCAGAAAA
    AGGGTGATAA AGATTCTAAG AGACATCTGT ATTGAACAAC CGACATTTCC AAAAATTACA
    GAAATGTGTG TAAAAATGAT TCGCAGGGTA AATGATGAAG AGGGCATTAA GAAATTAGTA
    AATGAAACAT TCCAAAAACT CTGGTTTACT CCAACTCCAC ACAATGACAA AGAAGCAATG
    ACAAGGAAGA TTTTAAATAT CACTGATGTG GTTGCAGCAT GCAGAGATAC TGGATATGAC
    TGGTTTGAGC AGCTTCTTCA AAATTTGTTG AAGTCTGAAG AGGATTCCTC ATATAAACCT
    GTGAAGAAAG CTTGTACTCA ACTTGTTGAT AACCTAGTTG AGCACATTCT TAAATATGAG
    GAATCTCTAG CTGACTCTGA CAATAAAGGT GTGAATTCTG GAAGATTGGT AGCTTGCATA
    ACCACTTTGT TCTTATTCAG CAAAATAAGA CCCCAGCTCA TGGTTAAACA TGCCATGACT
    ATGCAGCCAT ATCTTACCAC TAAATGTAGT ACACAAAATG ATTTCATGGT TATCTGCAAT
    GTTGCAAAAA TTCTAGAACT AGTTGTACCA CTGATGGAAC ATCCCAGTGA AACTTTCCTT
    GCCACTATTG AAGAAGATCT AATGAAGCTT ATCATCAAAT ATGGCATGAC TGTAGTGCAA
    CATTGTGTAA GCTGTCTTGG GGCTGTTGTA AATAAAGTGA CACAAAATTT TAAATTTGTG
    TGGGCCTGTT TCAATAGATA CTATGGTGCC ATTTCAAAAT TAAAAAGTCA ACACCAAGAG
    GACCCAAATA ACACTTCACT GCTAACAAAC AAACCAGCAC TTCTGAGATC CCTATTCACT
    GTTGGGGCAC TGTGTCGGCA TTTTGATTTT GATCTGGAAG ATTTTAAAGG CAACAGTAAG
    GTTAACATAA AGGATAAAGT CCTTGAACTA TTGATGTATT TTACAAAGCA TTCAGATGAA
    GAAGTGCAAA CAAAAGCCAT CATTGGTCTA GGATTTGCCT TTATTCAGCA CCCAAGTCTA
    ATGTTTGAGC AAGAGGTGAA AAATCTATAC AATAATATTT TATCTGATAA AAACTCCTCA
    GTAAATTTAA AAATACAAGT GTTAAAAAAC CTCCAGACCT ACCTACAAGA AGAAGATACA
    CGTATGCAGC AGGCAGATAG AGACTGGAAG AAGGTTGCAA AACAGGAAGA TTTGAAAGAA
    ATGGGTGATG TTTCCTCAGG GATGAGTAGT TCCATCATGC AGCTTTACCT CAAACAGGTG
    CTTGAGGCGT TTTTTCACAC CCAATCAAGT GTACGCCACT TCGCCCTCAA TGTCATTGCA
    TTGACTCTAA ACCAAGGTCT TATTCATCCA GTTCAGTGTG TGCCGTATTT AATTGCTATG
    GGCACAGACC CAGAACCAGC TATGCGGAAC AAGGCTGATC AGCAGCTTGT GGAAATAGAT
    AAAAAATATG CTGGATTCAT TCACATGAAA GCAGTGGCTG GCATGAAGAT GTCTTACCAG
    GTACAACAGG CAATCAACAC ATGCCTAAAA GATCCTGTAA GGGGTTTCCG GCAGGACGAG
    TCCTCCAGTG CTTTGTGTTC ACACCTTTAC TCCATGATCC GGGGAAACCG CCAGCACAGA
    CGCGCCTTTC TGATTTCTTT ACTCAACCTC TTTGATGACA CAGCAAAAAC AGAAGTGAAT
    ATGCTCTTGT ATATAGCAGA CAATCTAGCC TGTTTTCCAT ACCAGACACA GGAAGAGCCT
    TTGTTTATAA TGCATCATAT AGACATTACA CTCTCTGTTT CTGGTAGTAA CCTACTGCAG
    TCATTCAAGG AGTCTATGGT AAAAGACAAA AGGAAAGAGA GAAAATCATC TCCTGGTAAG
    GAAAATGAGT CAAGCGAGAG TGAAGAAGAA GTCTCTAGGC CTCGAAAGTC ACGGAAACGT
    GTAGATTCGG ATTCAGATTC GGATTCAGAA GATGATATAA ATTCGGTGAT GAAATGTTTG
    CCAGAAAACT CAGCTCCTTT AATTGAGTTT GCAAATGTCT CCCAGGGTAT TTTATTGCTT
    CTGATGTTAA AACAACATTT GAAGAATCTC TGTGGATTTT CTGATAGTAA AATTCAGAAA
    TATTCTCCAT CCGAATCTGC AAAAGTGTAT GATAAAGCGA TAAACCGGAA AACAGGAGTT
    CATTTTCATC CCAAGCAAAC ACTGGACTTC CTGCGGAGTG ATATGGCTAA TTCCAAAATC
    ACTGAAGATG TGAAGAGGAG TATAGTAAAA CAGTACCTAG ATTTCAAACT TCTCATGGAA
    CATCTGGACC CTGATGAAGA GGAAGAAGAA GGGGAGGTGT CAGCCAGCAC AAATGCTCGG
    AACAAAGCAA TTACCTCGCT GCTTGGAGGA GGCAGCCCTA AAAATAATGC AGCAGCAGAG
    ACAGAAGATG ATGAAAGTGA TGGGGAGGAT AGAGGAGGAG GCACTTCAGG GTCATTGAGA
    AGGTCAAAAC GAAATTCAGA CTCTACGGAG TTGGCAGCAC AGATGAATGA AAGTGTTGAC
    GTCATGGATG TCATCGCTAT TTGCTGTCCA AAGTACAAAG ACCGACCACA GATTGCAAGA
    GTAGTACAGA AAACCAGCAG TGGCTTCAGT GTTCAGTGGA TGGCAGGCTC CTACAGTGGC
    TCCTGGACTG AGGCTAAGCG CCGTGATGGC CGCAAACTGG TGCCTTGGGT AGACACTATT
    AAAGAGTCAG ACATTATTTA CAAAAAAATT GCTCTAACGA GTGCTAATAA GCTGACTAAT
    AAAGTTGTTC AGACTTTACG ATCCCTGTAT GCCGCCAAGG ATGGGACTTC CAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025126387.1
    CDS209..8425
    Translation

    Target ORF information:

    RefSeq Version XM_025270602.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis NIPBL, cohesin loading factor (NIPBL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025270602.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGTCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCTACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAACGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGACGCACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACGCCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGATTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAC CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAGGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAATGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGATAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATAAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCGCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA ATCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCCG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTCAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATTCGCTA GAAAAATGAA GAAAAAAGAA AAACAGAAGA AGCGGAAAGC TTATGAACCA
    AAACTAACAC CCGAAGAAAT GATGGATTCT TCAACTTTTA AGAGATTCAC AGCCTCAATA
    GAGAATATTT TGGATAACTT AGAAGATATG GATTTTACTG CATTTGGTGA TGATGATGAA
    ATTCCTCAGG AACTGCTCTT AGGAAAACAT CAGCTTAATG AACTTGGCAG TGAATCTGCT
    AAAATTAAAG CAATGGGTAT AATGGATAAG CTTTCCACTG ACAAAACTGT GAAAGTCCTA
    AATATCTTGG AAAAGAATAT TCAAGATGGG TCAAAGCTCT CCACTTTGTT AAATCATAAT
    AACGATACTG AAGAAGAAGA AAGGTTATGG AGAGACCTTA TTATGGAGAG AGTGACAAAA
    TCAGCAGATG CCTGTCTTAC AACTATAAAC ATCATGACAT CCCCTAACAT GCCAAAAGCT
    GTATATATTG AGGATGTAAT CGAAAGAGTT ATACAGTACA CTAAATTTCA TTTGCAGAAT
    ACACTTTATC CTCAGTATGA TCCTGTTTAC AGATTAGATC CTCATGGAGG AGGCTTACTA
    AGTTCAAAGG CAAAACGGGC TAAATGTTCT ACCCATAAGC AGAGAGTAAT AGTAATGCTT
    TATAATAAAG TTTGTGACAT TGTTAGCAGC TTATCAGAAC TACTAGAAAT ACAACTTCTA
    ACAGACACAA CAATCCTTCA GGTATCTTCA ATGGGAATAA CACCATTTTT TGTGGAAAAT
    GTCAGTGAAC TACAATTGTG TGCCATTAAA TTAGTCACTG CAGTATTCTC AAGATATGAA
    AAACATAGGC AGTTAATTTT GGAAGAAATT TTTACTTCAC TTGCAAGATT ACCTACCAGC
    AAGAGGAGCT TAAGGAACTT CAGGTTAAAT AGTAGTGATA TGGATGGAGA GCCTATGTAT
    ATTCAGATGG TTACAGCACT GGTTTTACAA CTGATTCAGT GTGTAGTACA CTTACCATCA
    TCAGAGAAGG ACTCTAATTC AGAAGAGGAC TCAAATAAAA AGGTTGACCA GGATGTTGTC
    ATTACCAATT CTTATGAAAC AGCCATGCGA ACAGCCCAAA ACTTCCTTTC CATCTTCCTT
    AAAAAATGTG GTAGTAAGCA AGGTGAAGAA GATTACAGAC CACTATTTGA AAATTTTGTT
    CAAGACCTTC TTTCAACAGT CAATAAGCCT GAATGGCCAG CTGCTGAACT ACTCCTTAGT
    TTGTTAGGGA GACTGTTGGT TCATCAGTTC AGTAATAAGT CAACAGAGAT GGCTTTAAGA
    GTGGCATCTC TTGATTACCT TGGCACTGTT GCTGCACGAC TGAGGAAAGA TGCTGTTACA
    AGCAAAATGG ATCAAGGGTC TATAGAACGA ATTTTAAAAC AGGTTTCAGG AGGGGAAGAT
    GAAATACAGC AATTGCAAAA AGCATTGCTT GATTACTTGG ATGAAAACAC GGAGACTGAT
    CCTTCACTGG TGTTTTCTCG TAAATTCTAT ATTGCCCAGT GGTTCCGTGA TACAACACTG
    GAAACAGAAA AAGCAATGAA ATCACAAAAA GATGAAGAAT CATCTGAAGG AGCACATCAT
    GCAAAGGAAG TTGAGACAAC TGGTCAAATA ATGCATCGAG CTGAAAACCG GAAGAAGTTT
    CTTCGAAGCA TTATCAAAAC CACTCCTTCT CAGTTCAGCA CATTAAAAAT GAACTCTGAT
    ACTGTGGACT ATGATGATGC TTGTTTGATT GTTCGATACT TGGCCTCTAT GAGGCCGTTT
    GCCCAGAGCT TTGATATTTA TTTGACACAG ATCCTTCGAG TCCTTGGTGA AAATGCAATT
    GCCGTTCGAA CAAAAGCCAT GAAGTGTTTA TCTGAGGTTG TTGCTGTAGA CCCCAGTATT
    CTAGCAAGGC TGGATATGCA GCGAGGAGTT CATGGACGAT TAATGGATAA CTCCACTAGT
    GTCAGAGAAG CAGCAGTGGA ATTACTAGGT CGATTTGTCC TTTGTCGACC TCAGCTTGCT
    GAGCAGTATT ATGATATGCT GATTGAAAGA ATATTGGATA CTGGTATCAG TGTCAGAAAA
    AGGGTGATAA AGATTCTAAG AGACATCTGT ATTGAACAAC CGACATTTCC AAAAATTACA
    GAAATGTGTG TAAAAATGAT TCGCAGGGTA AATGATGAAG AGGGCATTAA GAAATTAGTA
    AATGAAACAT TCCAAAAACT CTGGTTTACT CCAACTCCAC ACAATGACAA AGAAGCAATG
    ACAAGGAAGA TTTTAAATAT CACTGATGTG GTTGCAGCAT GCAGAGATAC TGGATATGAC
    TGGTTTGAGC AGCTTCTTCA AAATTTGTTG AAGTCTGAAG AGGATTCCTC ATATAAACCT
    GTGAAGAAAG CTTGTACTCA ACTTGTTGAT AACCTAGTTG AGCACATTCT TAAATATGAG
    GAATCTCTAG CTGACTCTGA CAATAAAGGT GTGAATTCTG GAAGATTGGT AGCTTGCATA
    ACCACTTTGT TCTTATTCAG CAAAATAAGA CCCCAGCTCA TGGTTAAACA TGCCATGACT
    ATGCAGCCAT ATCTTACCAC TAAATGTAGT ACACAAAATG ATTTCATGGT TATCTGCAAT
    GTTGCAAAAA TTCTAGAACT AGTTGTACCA CTGATGGAAC ATCCCAGTGA AACTTTCCTT
    GCCACTATTG AAGAAGATCT AATGAAGCTT ATCATCAAAT ATGGCATGAC TGTAGTGCAA
    CATTGTGTAA GCTGTCTTGG GGCTGTTGTA AATAAAGTGA CACAAAATTT TAAATTTGTG
    TGGGCCTGTT TCAATAGATA CTATGGTGCC ATTTCAAAAT TAAAAAGTCA ACACCAAGAG
    GACCCAAATA ACACTTCACT GCTAACAAAC AAACCAGCAC TTCTGAGATC CCTATTCACT
    GTTGGGGCAC TGTGTCGGCA TTTTGATTTT GATCTGGAAG ATTTTAAAGG CAACAGTAAG
    GTTAACATAA AGGATAAAGT CCTTGAACTA TTGATGTATT TTACAAAGCA TTCAGATGAA
    GAAGTGCAAA CAAAAGCCAT CATTGGTCTA GGATTTGCCT TTATTCAGCA CCCAAGTCTA
    ATGTTTGAGC AAGAGGTGAA AAATCTATAC AATAATATTT TATCTGATAA AAACTCCTCA
    GTAAATTTAA AAATACAAGT GTTAAAAAAC CTCCAGACCT ACCTACAAGA AGAAGATACA
    CGTATGCAGC AGGCAGATAG AGACTGGAAG AAGGTTGCAA AACAGGAAGA TTTGAAAGAA
    ATGGGTGATG TTTCCTCAGG GATGAGTAGT TCCATCATGC AGCTTTACCT CAAACAGGTG
    CTTGAGGCGT TTTTTCACAC CCAATCAAGT GTACGCCACT TCGCCCTCAA TGTCATTGCA
    TTGACTCTAA ACCAAGGTCT TATTCATCCA GTTCAGTGTG TGCCGTATTT AATTGCTATG
    GGCACAGACC CAGAACCAGC TATGCGGAAC AAGGCTGATC AGCAGCTTGT GGAAATAGAT
    AAAAAATATG CTGGATTCAT TCACATGAAA GCAGTGGCTG GCATGAAGAT GTCTTACCAG
    GTACAACAGG CAATCAACAC ATGCCTAAAA GATCCTGTAA GGGGTTTCCG GCAGGACGAG
    TCCTCCAGTG CTTTGTGTTC ACACCTTTAC TCCATGATCC GGGGAAACCG CCAGCACAGA
    CGCGCCTTTC TGATTTCTTT ACTCAACCTC TTTGATGACA CAGCAAAAAC AGAAGTGAAT
    ATGCTCTTGT ATATAGCAGA CAATCTAGCC TGTTTTCCAT ACCAGACACA GGAAGAGCCT
    TTGTTTATAA TGCATCATAT AGACATTACA CTCTCTGTTT CTGGTAGTAA CCTACTGCAG
    TCATTCAAGG AGTCTATGGT AAAAGACAAA AGGAAAGAGA GAAAATCATC TCCTGGTAAG
    GAAAATGAGT CAAGCGAGAG TGAAGAAGAA GTCTCTAGGC CTCGAAAGTC ACGGAAACGT
    GTAGATTCGG ATTCAGATTC GGATTCAGAA GATGATATAA ATTCGGTGAT GAAATGTTTG
    CCAGAAAACT CAGCTCCTTT AATTGAGTTT GCAAATGTCT CCCAGGGTAT TTTATTGCTT
    CTGATGTTAA AACAACATTT GAAGAATCTC TGTGGATTTT CTGATAGTAA AATTCAGAAA
    TATTCTCCAT CCGAATCTGC AAAAGTGTAT GATAAAGCGA TAAACCGGAA AACAGGAGTT
    CATTTTCATC CCAAGCAAAC ACTGGACTTC CTGCGGAGTG ATATGGCTAA TTCCAAAATC
    ACTGAAGATG TGAAGAGGAG TATAGTAAAA CAGTACCTAG ATTTCAAACT TCTCATGGAA
    CATCTGGACC CTGATGAAGA GGAAGAAGAA GGGGAGGTGT CAGCCAGCAC AAATGCTCGG
    AACAAAGCAA TTACCTCGCT GCTTGGAGGA GGCAGCCCTA AAAATAATGC AGCAGCAGAG
    ACAGAAGATG ATGAAAGTGA TGGGGAGGAT AGAGGAGGAG GCACTTCAGG GTCATTGAGA
    AGGTCAAAAC GAAATTCAGA CTCTACGGAG TTGGCAGCAC AGATGAATGA AAGTGTTGAC
    GTCATGGATG TCATCGCTAT TTGCTGTCCA AAGTACAAAG ACCGACCACA GATTGCAAGA
    GTAGTACAGA AAACCAGCAG TGGCTTCAGT GTTCAGTGGA TGGCAGGCTC CTACAGTGGC
    TCCTGGACTG AGGCTAAGCG CCGTGATGGC CGCAAACTGG TGCCTTGGGT AGACACTATT
    AAAGAGTCAG ACATTATTTA CAAAAAAATT GCTCTAACGA GTGCTAATAA GCTGACTAAT
    AAAGTTGTTC AGACTTTACG ATCCCTGTAT GCCGCCAAGG ATGGGACTTC CAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu53881
    Clone ID Related Accession (Same CDS sequence) XM_006059557.2
    Accession Version XM_006059557.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8097bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product nipped-B-like protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037563.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006059557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
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    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGTCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCTACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAACGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGACGCACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACGCCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGATTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAC CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAGGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAATGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGATAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATAAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCGCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA ATCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCCG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTCAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
    GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
    TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT
    CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG
    CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG
    AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA
    TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
    CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC
    AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC
    ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
    ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC
    CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT
    AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
    CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
    AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA
    CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA
    CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTCACTGCA
    GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
    GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG
    GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT GATTCAGTGT
    GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAG
    GTTGACCAGG ATGTTGTCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC
    TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
    CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
    GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA
    ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GCACTGTTGC TGCACGACTG
    AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG
    GTTTCAGGAG GGGAAGATGA AATACAGCAA TTGCAAAAAG CATTGCTTGA TTACTTGGAT
    GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG
    TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
    TCTGAAGGAG CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATAAT GCATCGAGCT
    GAAAACCGGA AGAAGTTTCT TCGAAGCATT ATCAAAACCA CTCCTTCTCA GTTCAGCACA
    TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG
    GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC
    CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT
    GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA
    ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT
    TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
    GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG
    ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG
    GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC
    AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC
    AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG
    GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
    CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
    AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
    GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT
    TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT
    CCCAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT
    GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA
    CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
    AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT
    CTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT
    TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTCC TTGAACTATT GATGTATTTT
    ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT
    ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA
    TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
    CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA
    CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
    CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC
    GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
    CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG
    CAGCTTGTGG AAATAGATAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC
    ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
    GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGG
    GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA
    GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
    CAGACACAGG AAGAGCCTTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT
    GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AAGACAAAAG GAAAGAGAGA
    AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT
    CGAAAGTCAC GGAAACGTGT AGATTCGGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT
    TCGGTGATGA AATGTTTGCC AGAAAACTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC
    CAGGGTATTT TATTGCTTCT GATGTTAAAA CAACATTTGA AGAATCTCTG TGGATTTTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA
    AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT
    ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT
    TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA
    GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
    ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC TCATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006059619.1
    CDS209..8305
    Translation

    Target ORF information:

    RefSeq Version XM_006059557.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis NIPBL, cohesin loading factor (NIPBL), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006059557.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGTCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAATGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCTACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAACGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGACGCACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACGCCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGATTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAC CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAGGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAATGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGATAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATAAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCGCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA ATCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCCG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTCAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
    GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT
    TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT
    CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG
    CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG
    AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA
    TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
    CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC
    AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC
    ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
    ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC
    CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT
    AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
    CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
    AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA
    CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA
    CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTCACTGCA
    GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT
    GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG
    GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT GATTCAGTGT
    GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAG
    GTTGACCAGG ATGTTGTCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC
    TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
    CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
    GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA
    ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GCACTGTTGC TGCACGACTG
    AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG
    GTTTCAGGAG GGGAAGATGA AATACAGCAA TTGCAAAAAG CATTGCTTGA TTACTTGGAT
    GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG
    TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
    TCTGAAGGAG CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATAAT GCATCGAGCT
    GAAAACCGGA AGAAGTTTCT TCGAAGCATT ATCAAAACCA CTCCTTCTCA GTTCAGCACA
    TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG
    GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC
    CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT
    GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA
    ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT
    TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
    GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG
    ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG
    GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC
    AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC
    AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG
    GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
    CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
    AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
    GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT
    TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT
    CCCAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT
    GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA
    CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
    AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT
    CTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT
    TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTCC TTGAACTATT GATGTATTTT
    ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT
    ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA
    TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
    CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA
    CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
    CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC
    GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
    CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG
    CAGCTTGTGG AAATAGATAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC
    ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG
    GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGG
    GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA
    GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
    CAGACACAGG AAGAGCCTTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT
    GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AAGACAAAAG GAAAGAGAGA
    AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT
    CGAAAGTCAC GGAAACGTGT AGATTCGGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT
    TCGGTGATGA AATGTTTGCC AGAAAACTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC
    CAGGGTATTT TATTGCTTCT GATGTTAAAA CAACATTTGA AGAATCTCTG TGGATTTTCT
    GATAGTAAAA TTCAGAAATA TTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA
    AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT
    ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT
    TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA
    GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
    ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC TCATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.