COL4A4 gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006047130.1	XP_006047192.1	collagen alpha-4(IV) chain isoform X4
XM_006047136.1	XP_006047198.1	collagen alpha-4(IV) chain isoform X10
XM_006047127.2	XP_006047189.1	collagen alpha-4(IV) chain isoform X1
XM_006047132.1	XP_006047194.1	collagen alpha-4(IV) chain isoform X6
XM_006047128.1	XP_006047190.1	collagen alpha-4(IV) chain isoform X2
XM_006047128.2	XP_006047190.1	collagen alpha-4(IV) chain isoform X1
XM_006047130.2	XP_006047192.1	collagen alpha-4(IV) chain isoform X1
XM_006047129.1	XP_006047191.1	collagen alpha-4(IV) chain isoform X3
XM_006047131.1	XP_006047193.1	collagen alpha-4(IV) chain isoform X5
XM_006047133.1	XP_006047195.1	collagen alpha-4(IV) chain isoform X7
XM_006047134.1	XP_006047196.1	collagen alpha-4(IV) chain isoform X8
XM_006047129.2	XP_006047191.1	collagen alpha-4(IV) chain isoform X1
XM_006047136.2	XP_006047198.1	collagen alpha-4(IV) chain isoform X4
XM_006047127.1	XP_006047189.1	collagen alpha-4(IV) chain isoform X1
XM_025262088.1	XP_025117873.1	collagen alpha-4(IV) chain isoform X2
XM_006047135.2	XP_006047197.1	collagen alpha-4(IV) chain isoform X3
XM_006047135.1	XP_006047197.1	collagen alpha-4(IV) chain isoform X9
XM_006047131.2	XP_006047193.1	collagen alpha-4(IV) chain isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu54413	XM_025262088.1 Latest version!	Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu05808	XM_006047127.2 Latest version!	Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBu05808	XM_006047130.2 Latest version!	Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBu05808	XM_006047128.2 Latest version!	Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBu05808	XM_006047131.2 Latest version!	Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBu05808	XM_006047129.2 Latest version!	Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBu05809	XM_006047132.1 Latest version!	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBu05810	XM_006047133.1 Latest version!	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBu05811	XM_006047134.1 Latest version!	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBu05812	XM_006047135.2 Latest version!	Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBu05813	XM_006047136.2 Latest version!	Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
View Old Accession Versions >>
OBu05808	XM_006047128.1	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBu05808	XM_006047130.1	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBu05808	XM_006047129.1	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBu05813	XM_006047136.1	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBu05808	XM_006047127.1	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBu05812	XM_006047135.1	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBu05808	XM_006047131.1	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

COL4A4 ( XM_025262088.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117873.1 Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBu54413
Clone ID Related Accession (Same CDS sequence)	XM_025262088.1
Accession Version	XM_025262088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-4(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGGATT CTCCAGGAAA GAACACTAGA GTGGATTGCC AGGCCCCCCC TCCAGGGCAT 
CTTCTCGACC CAGGGACTGA AGCCATGTCT CATTATGTCT CCGGCATTGC AGGCAGGTTT 
TTTACCATTA GGGCCACCTG GAAAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117873.1
CDS	248..5197
Translation	MALVRCPFRCNKLLATNSWSLILILFSVQYAYGGGKKFIGLCGGRDCSLCQCFPEKGSRGQPGPPGPQGPIGPLGPPGPIGIPGEKGMKGDSGRPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGSRGRPGIDGYNGSRGDPGIPGERGALGPRGPPGLPGEKGEKGNSVFILGATKGMQGDRGDPGPPGSPGSRGVRGPAGPAGYPGEPGLAGPPGPPGSPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVQPPDSGLYKGEKGIKGMPGMTGPPGPLGPRGEPGIGRKGEKGIPGFPGPRGDPGSYGSPGFPGLKGEPGLFGDPGSFGFLGPKGDPGDRGHPGPPGVLVTPSLPLKGPPGDPGHPGRYGETGAVGPRGPPGAPGRPGEACPGMTGPPGPRGFPGHPGFPGAAGVPGRADSAPGKPGNQGPTGLPGVPGLPGPPGSDVVYCTVGQPGPQGIKGKVGPPGRRGSKGEKGSSGLCACQPGPMGPSGPPGPPGRQGSKGDLGLPGWLGEKGSPGPPGAKGSPGPPGKPGASGLPGSKGEKGDMVVSRVKGHKGERGPDGPPGLPGQQGQHGRDGYAGEKGDTGPPGDHEDAAPGDKGPPGPPGPPGRAGPRGPPGLGFPGPPGDRGQPGAPGCPGNRGPDGRKGRKGDTIPCNVTYPGRPGPPGFDGPPGPKGLPGHRGAPGLRCLDGQKGQRGKPGISERPGPPGFRGDIGEPGLGGEKGSSPVGPPGAPGSPGVNGQKGHPGDPAIGYPGLPGKSGLPGAPGSKGLRGDAGRPGTTGPAGMPGFPGLQGPKGREGNAGFPGIPGLPGHSCERGAPGIPGQPGLPGAPGSPGAPGWKGQRGDMGPLGPAGMKGLPGVPGWPGADGTPGLPGVPGPTGDDGLPGLPGPKGPQGLPGFLGFPGERGKPGQDGHPGRKGEHGEKGWPGFLGDRGLKGVKGARGPPGYEGEMDIISKKGETGEPGASGDGGFPGENGDKGHPGMQGRRGEPGSHGPPGFHPGEPGRNGQPGLPGPPGPPGSPGLRGNIGFPGFPGDQGEPGSPGCPGPSGMDGTRGPKGYKGESASQFGLPGPKGKPGGPGYPGRLGASGEQGLPGAQGPGGPPGRPGPPGLFGPTGCPGSPGGPGLQGPPGEAGDPGPRGVLGDPGAPGLPGIKGPSGSPGLNGLHGLKGQKGSKGASGSHEMGPPGPVGMPGPKGETGDPGNPGISPPGLFGERGAPGPPGPPGRAGLPGPPGAPGKALKGDIPDTGLPGDQGPPGPDGPRGVPGPPGPPGSVDLLKGEPGDCGLPGPPGLPGPPGPPGHKGFPGCDGKHGQKGPMGFPGPQGPPGSPGPPGDKGLPGPPGRRGPLGPPGSRGEPGPPADLDACPRIPGLPGVPGPRGPEGTMGLPGMRGPPGPGCKGEPGLDGRRGEDGLPGSPGPPGHKGDMGEAGCPGAPGPPGPMGDPGPIGFGPGYLSGFLLVLHSQTDGEPTCPMGMPRLWTGYSLLYLEGQERAHNQDLGLAGSCLPIFSTLPFAYCNIHQVCHYARRNDRSYWLASTAPLPMTPLSEDEIRPYISRCAVCEAPAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMDSPGKNTRVDCQAPPPGHLLDPGTEAMSHYVSGIAGRFFTIRATWKA

Target ORF information:

RefSeq Version	XM_025262088.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025262088.1

ORF Insert Sequence:

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGGATT CTCCAGGAAA GAACACTAGA GTGGATTGCC AGGCCCCCCC TCCAGGGCAT 
CTTCTCGACC CAGGGACTGA AGCCATGTCT CATTATGTCT CCGGCATTGC AGGCAGGTTT 
TTTACCATTA GGGCCACCTG GAAAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_006047130.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047192.1 Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu05808
Clone ID Related Accession (Same CDS sequence)	XM_006047130.1 , XM_006047127.2 , XM_006047128.1 , XM_006047128.2 , XM_006047130.2 , XM_006047129.1 , XM_006047131.1 , XM_006047129.2 , XM_006047127.1 , XM_006047131.2
Accession Version	XM_006047130.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-4(IV) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047192.1
CDS	218..5287
Translation	MALVRCPFRCNKLLATNSWSLILILFSVQYAYGGGKKFIGLCGGRDCSLCQCFPEKGSRGQPGPPGPQGPIGPLGPPGPIGIPGEKGMKGDSGRPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGSRGRPGIDGYNGSRGDPGIPGERGALGPRGPPGLPGEKGEKGNSVFILGATKGMQGDRGDPGPPGSPGSRGVRGPAGPAGYPGEPGLAGPPGPPGSPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVQPPDSGLYKGEKGIKGMPGMTGPPGPLGPRGEPGIGRKGEKGIPGFPGPRGDPGSYGSPGFPGLKGEPGLFGDPGSFGFLGPKGDPGDRGHPGPPGVLVTPSLPLKGPPGDPGHPGRYGETGAVGPRGPPGAPGRPGEACPGMTGPPGPRGFPGHPGFPGAAGVPGRADSAPGKPGNQGPTGLPGVPGLPGPPGSDVVYCTVGQPGPQGIKGKVGPPGRRGSKGEKGSSGLCACQPGPMGPSGPPGPPGRQGSKGDLGLPGWLGEKGSPGPPGAKGSPGPPGKPGASGLPGSKGEKGDMVVSRVKGHKGERGPDGPPGLPGQQGQHGRDGYAGEKGDTGPPGDHEDAAPGDKGPPGPPGPPGRAGPRGPPGLGFPGPPGDRGQPGAPGCPGNRGPDGRKGRKGDTIPCNVTYPGRPGPPGFDGPPGPKGLPGHRGAPGLRCLDGQKGQRGKPGISERPGPPGFRGDIGEPGLGGEKGSSPVGPPGAPGSPGVNGQKGHPGDPAIGYPGLPGKSGLPGAPGSKGLRGDAGRPGTTGPAGMPGFPGLQGPKGREGNAGFPGIPGLPGHSCERGAPGIPGQPGLPGAPGSPGAPGWKGQRGDMGPLGPAGMKGLPGVPGWPGADGTPGLPGVPGPTGDDGLPGLPGPKGPQGLPGFLGFPGERGKPGQDGHPGRKGEHGEKGWPGFLGDRGLKGVKGARGPPGYEGEMDIISKKGETGEPGASGDGGFPGENGDKGHPGMQGRRGEPGSHGPPGFHPGEPGRNGQPGLPGPPGPPGSPGLRGNIGFPGFPGDQGEPGSPGCPGPSGMDGTRGPKGYKGESASQFGLPGPKGKPGGPGYPGRLGASGEQGLPGAQGPGGPPGRPGPPGLFGPTGCPGSPGGPGLQGPPGEAGDPGPRGVLGDPGAPGLPGIKGPSGSPGLNGLHGLKGQKGSKGASGSHEMGPPGPVGMPGPKGETGDPGNPGISPPGLFGERGAPGPPGPPGRAGLPGPPGAPGKALKGDIPDTGLPGDQGPPGPDGPRGVPGPPGPPGSVDLLKGEPGDCGLPGPPGLPGPPGPPGHKGFPGCDGKHGQKGPMGFPGPQGPPGSPGPPGDKGLPGPPGRRGPLGPPGSRGEPGPPADLDACPRIPGLPGVPGPRGPEGTMGLPGMRGPPGPGCKGEPGLDGRRGEDGLPGSPGPPGHKGDMGEAGCPGAPGPPGPMGDPGPIGFGPGYLSGFLLVLHSQTDGEPTCPMGMPRLWTGYSLLYLEGQERAHNQDLGLAGSCLPIFSTLPFAYCNIHQVCHYARRNDRSYWLASTAPLPMTPLSEDEIRPYISRCAVCEAPAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVRPDLQFSSAPLPDTLKESHAQRQKISRCQVCVKHS

Target ORF information:

RefSeq Version	XM_006047130.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047130.1

ORF Insert Sequence:

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_006047127.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047189.1 Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu05808
Clone ID Related Accession (Same CDS sequence)	XM_006047130.1 , XM_006047127.2 , XM_006047128.1 , XM_006047128.2 , XM_006047130.2 , XM_006047129.1 , XM_006047131.1 , XM_006047129.2 , XM_006047127.1 , XM_006047131.2
Accession Version	XM_006047127.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006047127.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047189.1
CDS	253..5322
Translation	MALVRCPFRCNKLLATNSWSLILILFSVQYAYGGGKKFIGLCGGRDCSLCQCFPEKGSRGQPGPPGPQGPIGPLGPPGPIGIPGEKGMKGDSGRPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGSRGRPGIDGYNGSRGDPGIPGERGALGPRGPPGLPGEKGEKGNSVFILGATKGMQGDRGDPGPPGSPGSRGVRGPAGPAGYPGEPGLAGPPGPPGSPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVQPPDSGLYKGEKGIKGMPGMTGPPGPLGPRGEPGIGRKGEKGIPGFPGPRGDPGSYGSPGFPGLKGEPGLFGDPGSFGFLGPKGDPGDRGHPGPPGVLVTPSLPLKGPPGDPGHPGRYGETGAVGPRGPPGAPGRPGEACPGMTGPPGPRGFPGHPGFPGAAGVPGRADSAPGKPGNQGPTGLPGVPGLPGPPGSDVVYCTVGQPGPQGIKGKVGPPGRRGSKGEKGSSGLCACQPGPMGPSGPPGPPGRQGSKGDLGLPGWLGEKGSPGPPGAKGSPGPPGKPGASGLPGSKGEKGDMVVSRVKGHKGERGPDGPPGLPGQQGQHGRDGYAGEKGDTGPPGDHEDAAPGDKGPPGPPGPPGRAGPRGPPGLGFPGPPGDRGQPGAPGCPGNRGPDGRKGRKGDTIPCNVTYPGRPGPPGFDGPPGPKGLPGHRGAPGLRCLDGQKGQRGKPGISERPGPPGFRGDIGEPGLGGEKGSSPVGPPGAPGSPGVNGQKGHPGDPAIGYPGLPGKSGLPGAPGSKGLRGDAGRPGTTGPAGMPGFPGLQGPKGREGNAGFPGIPGLPGHSCERGAPGIPGQPGLPGAPGSPGAPGWKGQRGDMGPLGPAGMKGLPGVPGWPGADGTPGLPGVPGPTGDDGLPGLPGPKGPQGLPGFLGFPGERGKPGQDGHPGRKGEHGEKGWPGFLGDRGLKGVKGARGPPGYEGEMDIISKKGETGEPGASGDGGFPGENGDKGHPGMQGRRGEPGSHGPPGFHPGEPGRNGQPGLPGPPGPPGSPGLRGNIGFPGFPGDQGEPGSPGCPGPSGMDGTRGPKGYKGESASQFGLPGPKGKPGGPGYPGRLGASGEQGLPGAQGPGGPPGRPGPPGLFGPTGCPGSPGGPGLQGPPGEAGDPGPRGVLGDPGAPGLPGIKGPSGSPGLNGLHGLKGQKGSKGASGSHEMGPPGPVGMPGPKGETGDPGNPGISPPGLFGERGAPGPPGPPGRAGLPGPPGAPGKALKGDIPDTGLPGDQGPPGPDGPRGVPGPPGPPGSVDLLKGEPGDCGLPGPPGLPGPPGPPGHKGFPGCDGKHGQKGPMGFPGPQGPPGSPGPPGDKGLPGPPGRRGPLGPPGSRGEPGPPADLDACPRIPGLPGVPGPRGPEGTMGLPGMRGPPGPGCKGEPGLDGRRGEDGLPGSPGPPGHKGDMGEAGCPGAPGPPGPMGDPGPIGFGPGYLSGFLLVLHSQTDGEPTCPMGMPRLWTGYSLLYLEGQERAHNQDLGLAGSCLPIFSTLPFAYCNIHQVCHYARRNDRSYWLASTAPLPMTPLSEDEIRPYISRCAVCEAPAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVRPDLQFSSAPLPDTLKESHAQRQKISRCQVCVKHS

Target ORF information:

RefSeq Version	XM_006047127.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047127.2

ORF Insert Sequence:

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_006047128.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047190.1 Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X2, mRNA.

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CloneID	OBu05808
Clone ID Related Accession (Same CDS sequence)	XM_006047130.1 , XM_006047127.2 , XM_006047128.1 , XM_006047128.2 , XM_006047130.2 , XM_006047129.1 , XM_006047131.1 , XM_006047129.2 , XM_006047127.1 , XM_006047131.2
Accession Version	XM_006047128.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-4(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047190.1
CDS	221..5290
Translation	MALVRCPFRCNKLLATNSWSLILILFSVQYAYGGGKKFIGLCGGRDCSLCQCFPEKGSRGQPGPPGPQGPIGPLGPPGPIGIPGEKGMKGDSGRPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGSRGRPGIDGYNGSRGDPGIPGERGALGPRGPPGLPGEKGEKGNSVFILGATKGMQGDRGDPGPPGSPGSRGVRGPAGPAGYPGEPGLAGPPGPPGSPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVQPPDSGLYKGEKGIKGMPGMTGPPGPLGPRGEPGIGRKGEKGIPGFPGPRGDPGSYGSPGFPGLKGEPGLFGDPGSFGFLGPKGDPGDRGHPGPPGVLVTPSLPLKGPPGDPGHPGRYGETGAVGPRGPPGAPGRPGEACPGMTGPPGPRGFPGHPGFPGAAGVPGRADSAPGKPGNQGPTGLPGVPGLPGPPGSDVVYCTVGQPGPQGIKGKVGPPGRRGSKGEKGSSGLCACQPGPMGPSGPPGPPGRQGSKGDLGLPGWLGEKGSPGPPGAKGSPGPPGKPGASGLPGSKGEKGDMVVSRVKGHKGERGPDGPPGLPGQQGQHGRDGYAGEKGDTGPPGDHEDAAPGDKGPPGPPGPPGRAGPRGPPGLGFPGPPGDRGQPGAPGCPGNRGPDGRKGRKGDTIPCNVTYPGRPGPPGFDGPPGPKGLPGHRGAPGLRCLDGQKGQRGKPGISERPGPPGFRGDIGEPGLGGEKGSSPVGPPGAPGSPGVNGQKGHPGDPAIGYPGLPGKSGLPGAPGSKGLRGDAGRPGTTGPAGMPGFPGLQGPKGREGNAGFPGIPGLPGHSCERGAPGIPGQPGLPGAPGSPGAPGWKGQRGDMGPLGPAGMKGLPGVPGWPGADGTPGLPGVPGPTGDDGLPGLPGPKGPQGLPGFLGFPGERGKPGQDGHPGRKGEHGEKGWPGFLGDRGLKGVKGARGPPGYEGEMDIISKKGETGEPGASGDGGFPGENGDKGHPGMQGRRGEPGSHGPPGFHPGEPGRNGQPGLPGPPGPPGSPGLRGNIGFPGFPGDQGEPGSPGCPGPSGMDGTRGPKGYKGESASQFGLPGPKGKPGGPGYPGRLGASGEQGLPGAQGPGGPPGRPGPPGLFGPTGCPGSPGGPGLQGPPGEAGDPGPRGVLGDPGAPGLPGIKGPSGSPGLNGLHGLKGQKGSKGASGSHEMGPPGPVGMPGPKGETGDPGNPGISPPGLFGERGAPGPPGPPGRAGLPGPPGAPGKALKGDIPDTGLPGDQGPPGPDGPRGVPGPPGPPGSVDLLKGEPGDCGLPGPPGLPGPPGPPGHKGFPGCDGKHGQKGPMGFPGPQGPPGSPGPPGDKGLPGPPGRRGPLGPPGSRGEPGPPADLDACPRIPGLPGVPGPRGPEGTMGLPGMRGPPGPGCKGEPGLDGRRGEDGLPGSPGPPGHKGDMGEAGCPGAPGPPGPMGDPGPIGFGPGYLSGFLLVLHSQTDGEPTCPMGMPRLWTGYSLLYLEGQERAHNQDLGLAGSCLPIFSTLPFAYCNIHQVCHYARRNDRSYWLASTAPLPMTPLSEDEIRPYISRCAVCEAPAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVRPDLQFSSAPLPDTLKESHAQRQKISRCQVCVKHS

Target ORF information:

RefSeq Version	XM_006047128.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047128.1

ORF Insert Sequence:

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_006047128.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047190.1 Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA.

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CloneID	OBu05808
Clone ID Related Accession (Same CDS sequence)	XM_006047130.1 , XM_006047127.2 , XM_006047128.1 , XM_006047128.2 , XM_006047130.2 , XM_006047129.1 , XM_006047131.1 , XM_006047129.2 , XM_006047127.1 , XM_006047131.2
Accession Version	XM_006047128.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006047128.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047190.1
CDS	256..5325
Translation	MALVRCPFRCNKLLATNSWSLILILFSVQYAYGGGKKFIGLCGGRDCSLCQCFPEKGSRGQPGPPGPQGPIGPLGPPGPIGIPGEKGMKGDSGRPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGSRGRPGIDGYNGSRGDPGIPGERGALGPRGPPGLPGEKGEKGNSVFILGATKGMQGDRGDPGPPGSPGSRGVRGPAGPAGYPGEPGLAGPPGPPGSPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVQPPDSGLYKGEKGIKGMPGMTGPPGPLGPRGEPGIGRKGEKGIPGFPGPRGDPGSYGSPGFPGLKGEPGLFGDPGSFGFLGPKGDPGDRGHPGPPGVLVTPSLPLKGPPGDPGHPGRYGETGAVGPRGPPGAPGRPGEACPGMTGPPGPRGFPGHPGFPGAAGVPGRADSAPGKPGNQGPTGLPGVPGLPGPPGSDVVYCTVGQPGPQGIKGKVGPPGRRGSKGEKGSSGLCACQPGPMGPSGPPGPPGRQGSKGDLGLPGWLGEKGSPGPPGAKGSPGPPGKPGASGLPGSKGEKGDMVVSRVKGHKGERGPDGPPGLPGQQGQHGRDGYAGEKGDTGPPGDHEDAAPGDKGPPGPPGPPGRAGPRGPPGLGFPGPPGDRGQPGAPGCPGNRGPDGRKGRKGDTIPCNVTYPGRPGPPGFDGPPGPKGLPGHRGAPGLRCLDGQKGQRGKPGISERPGPPGFRGDIGEPGLGGEKGSSPVGPPGAPGSPGVNGQKGHPGDPAIGYPGLPGKSGLPGAPGSKGLRGDAGRPGTTGPAGMPGFPGLQGPKGREGNAGFPGIPGLPGHSCERGAPGIPGQPGLPGAPGSPGAPGWKGQRGDMGPLGPAGMKGLPGVPGWPGADGTPGLPGVPGPTGDDGLPGLPGPKGPQGLPGFLGFPGERGKPGQDGHPGRKGEHGEKGWPGFLGDRGLKGVKGARGPPGYEGEMDIISKKGETGEPGASGDGGFPGENGDKGHPGMQGRRGEPGSHGPPGFHPGEPGRNGQPGLPGPPGPPGSPGLRGNIGFPGFPGDQGEPGSPGCPGPSGMDGTRGPKGYKGESASQFGLPGPKGKPGGPGYPGRLGASGEQGLPGAQGPGGPPGRPGPPGLFGPTGCPGSPGGPGLQGPPGEAGDPGPRGVLGDPGAPGLPGIKGPSGSPGLNGLHGLKGQKGSKGASGSHEMGPPGPVGMPGPKGETGDPGNPGISPPGLFGERGAPGPPGPPGRAGLPGPPGAPGKALKGDIPDTGLPGDQGPPGPDGPRGVPGPPGPPGSVDLLKGEPGDCGLPGPPGLPGPPGPPGHKGFPGCDGKHGQKGPMGFPGPQGPPGSPGPPGDKGLPGPPGRRGPLGPPGSRGEPGPPADLDACPRIPGLPGVPGPRGPEGTMGLPGMRGPPGPGCKGEPGLDGRRGEDGLPGSPGPPGHKGDMGEAGCPGAPGPPGPMGDPGPIGFGPGYLSGFLLVLHSQTDGEPTCPMGMPRLWTGYSLLYLEGQERAHNQDLGLAGSCLPIFSTLPFAYCNIHQVCHYARRNDRSYWLASTAPLPMTPLSEDEIRPYISRCAVCEAPAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVRPDLQFSSAPLPDTLKESHAQRQKISRCQVCVKHS

Target ORF information:

RefSeq Version	XM_006047128.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047128.2

ORF Insert Sequence:

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_006047130.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047192.1 Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X4, mRNA.

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CloneID	OBu05808
Clone ID Related Accession (Same CDS sequence)	XM_006047130.1 , XM_006047127.2 , XM_006047128.1 , XM_006047128.2 , XM_006047130.2 , XM_006047129.1 , XM_006047131.1 , XM_006047129.2 , XM_006047127.1 , XM_006047131.2
Accession Version	XM_006047130.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006047130.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047192.1
CDS	372..5441
Translation	MALVRCPFRCNKLLATNSWSLILILFSVQYAYGGGKKFIGLCGGRDCSLCQCFPEKGSRGQPGPPGPQGPIGPLGPPGPIGIPGEKGMKGDSGRPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGSRGRPGIDGYNGSRGDPGIPGERGALGPRGPPGLPGEKGEKGNSVFILGATKGMQGDRGDPGPPGSPGSRGVRGPAGPAGYPGEPGLAGPPGPPGSPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVQPPDSGLYKGEKGIKGMPGMTGPPGPLGPRGEPGIGRKGEKGIPGFPGPRGDPGSYGSPGFPGLKGEPGLFGDPGSFGFLGPKGDPGDRGHPGPPGVLVTPSLPLKGPPGDPGHPGRYGETGAVGPRGPPGAPGRPGEACPGMTGPPGPRGFPGHPGFPGAAGVPGRADSAPGKPGNQGPTGLPGVPGLPGPPGSDVVYCTVGQPGPQGIKGKVGPPGRRGSKGEKGSSGLCACQPGPMGPSGPPGPPGRQGSKGDLGLPGWLGEKGSPGPPGAKGSPGPPGKPGASGLPGSKGEKGDMVVSRVKGHKGERGPDGPPGLPGQQGQHGRDGYAGEKGDTGPPGDHEDAAPGDKGPPGPPGPPGRAGPRGPPGLGFPGPPGDRGQPGAPGCPGNRGPDGRKGRKGDTIPCNVTYPGRPGPPGFDGPPGPKGLPGHRGAPGLRCLDGQKGQRGKPGISERPGPPGFRGDIGEPGLGGEKGSSPVGPPGAPGSPGVNGQKGHPGDPAIGYPGLPGKSGLPGAPGSKGLRGDAGRPGTTGPAGMPGFPGLQGPKGREGNAGFPGIPGLPGHSCERGAPGIPGQPGLPGAPGSPGAPGWKGQRGDMGPLGPAGMKGLPGVPGWPGADGTPGLPGVPGPTGDDGLPGLPGPKGPQGLPGFLGFPGERGKPGQDGHPGRKGEHGEKGWPGFLGDRGLKGVKGARGPPGYEGEMDIISKKGETGEPGASGDGGFPGENGDKGHPGMQGRRGEPGSHGPPGFHPGEPGRNGQPGLPGPPGPPGSPGLRGNIGFPGFPGDQGEPGSPGCPGPSGMDGTRGPKGYKGESASQFGLPGPKGKPGGPGYPGRLGASGEQGLPGAQGPGGPPGRPGPPGLFGPTGCPGSPGGPGLQGPPGEAGDPGPRGVLGDPGAPGLPGIKGPSGSPGLNGLHGLKGQKGSKGASGSHEMGPPGPVGMPGPKGETGDPGNPGISPPGLFGERGAPGPPGPPGRAGLPGPPGAPGKALKGDIPDTGLPGDQGPPGPDGPRGVPGPPGPPGSVDLLKGEPGDCGLPGPPGLPGPPGPPGHKGFPGCDGKHGQKGPMGFPGPQGPPGSPGPPGDKGLPGPPGRRGPLGPPGSRGEPGPPADLDACPRIPGLPGVPGPRGPEGTMGLPGMRGPPGPGCKGEPGLDGRRGEDGLPGSPGPPGHKGDMGEAGCPGAPGPPGPMGDPGPIGFGPGYLSGFLLVLHSQTDGEPTCPMGMPRLWTGYSLLYLEGQERAHNQDLGLAGSCLPIFSTLPFAYCNIHQVCHYARRNDRSYWLASTAPLPMTPLSEDEIRPYISRCAVCEAPAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVRPDLQFSSAPLPDTLKESHAQRQKISRCQVCVKHS

Target ORF information:

RefSeq Version	XM_006047130.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047130.2

ORF Insert Sequence:

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_006047129.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047191.1 Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X3, mRNA.

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CloneID	OBu05808
Clone ID Related Accession (Same CDS sequence)	XM_006047130.1 , XM_006047127.2 , XM_006047128.1 , XM_006047128.2 , XM_006047130.2 , XM_006047129.1 , XM_006047131.1 , XM_006047129.2 , XM_006047127.1 , XM_006047131.2
Accession Version	XM_006047129.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-4(IV) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047191.1
CDS	258..5327
Translation	MALVRCPFRCNKLLATNSWSLILILFSVQYAYGGGKKFIGLCGGRDCSLCQCFPEKGSRGQPGPPGPQGPIGPLGPPGPIGIPGEKGMKGDSGRPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGSRGRPGIDGYNGSRGDPGIPGERGALGPRGPPGLPGEKGEKGNSVFILGATKGMQGDRGDPGPPGSPGSRGVRGPAGPAGYPGEPGLAGPPGPPGSPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVQPPDSGLYKGEKGIKGMPGMTGPPGPLGPRGEPGIGRKGEKGIPGFPGPRGDPGSYGSPGFPGLKGEPGLFGDPGSFGFLGPKGDPGDRGHPGPPGVLVTPSLPLKGPPGDPGHPGRYGETGAVGPRGPPGAPGRPGEACPGMTGPPGPRGFPGHPGFPGAAGVPGRADSAPGKPGNQGPTGLPGVPGLPGPPGSDVVYCTVGQPGPQGIKGKVGPPGRRGSKGEKGSSGLCACQPGPMGPSGPPGPPGRQGSKGDLGLPGWLGEKGSPGPPGAKGSPGPPGKPGASGLPGSKGEKGDMVVSRVKGHKGERGPDGPPGLPGQQGQHGRDGYAGEKGDTGPPGDHEDAAPGDKGPPGPPGPPGRAGPRGPPGLGFPGPPGDRGQPGAPGCPGNRGPDGRKGRKGDTIPCNVTYPGRPGPPGFDGPPGPKGLPGHRGAPGLRCLDGQKGQRGKPGISERPGPPGFRGDIGEPGLGGEKGSSPVGPPGAPGSPGVNGQKGHPGDPAIGYPGLPGKSGLPGAPGSKGLRGDAGRPGTTGPAGMPGFPGLQGPKGREGNAGFPGIPGLPGHSCERGAPGIPGQPGLPGAPGSPGAPGWKGQRGDMGPLGPAGMKGLPGVPGWPGADGTPGLPGVPGPTGDDGLPGLPGPKGPQGLPGFLGFPGERGKPGQDGHPGRKGEHGEKGWPGFLGDRGLKGVKGARGPPGYEGEMDIISKKGETGEPGASGDGGFPGENGDKGHPGMQGRRGEPGSHGPPGFHPGEPGRNGQPGLPGPPGPPGSPGLRGNIGFPGFPGDQGEPGSPGCPGPSGMDGTRGPKGYKGESASQFGLPGPKGKPGGPGYPGRLGASGEQGLPGAQGPGGPPGRPGPPGLFGPTGCPGSPGGPGLQGPPGEAGDPGPRGVLGDPGAPGLPGIKGPSGSPGLNGLHGLKGQKGSKGASGSHEMGPPGPVGMPGPKGETGDPGNPGISPPGLFGERGAPGPPGPPGRAGLPGPPGAPGKALKGDIPDTGLPGDQGPPGPDGPRGVPGPPGPPGSVDLLKGEPGDCGLPGPPGLPGPPGPPGHKGFPGCDGKHGQKGPMGFPGPQGPPGSPGPPGDKGLPGPPGRRGPLGPPGSRGEPGPPADLDACPRIPGLPGVPGPRGPEGTMGLPGMRGPPGPGCKGEPGLDGRRGEDGLPGSPGPPGHKGDMGEAGCPGAPGPPGPMGDPGPIGFGPGYLSGFLLVLHSQTDGEPTCPMGMPRLWTGYSLLYLEGQERAHNQDLGLAGSCLPIFSTLPFAYCNIHQVCHYARRNDRSYWLASTAPLPMTPLSEDEIRPYISRCAVCEAPAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVRPDLQFSSAPLPDTLKESHAQRQKISRCQVCVKHS

Target ORF information:

RefSeq Version	XM_006047129.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047129.1

ORF Insert Sequence:

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_006047131.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047193.1 Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X5, mRNA.

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CloneID	OBu05808
Clone ID Related Accession (Same CDS sequence)	XM_006047130.1 , XM_006047127.2 , XM_006047128.1 , XM_006047128.2 , XM_006047130.2 , XM_006047129.1 , XM_006047131.1 , XM_006047129.2 , XM_006047127.1 , XM_006047131.2
Accession Version	XM_006047131.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-4(IV) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047193.1
CDS	137..5206
Translation	MALVRCPFRCNKLLATNSWSLILILFSVQYAYGGGKKFIGLCGGRDCSLCQCFPEKGSRGQPGPPGPQGPIGPLGPPGPIGIPGEKGMKGDSGRPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGSRGRPGIDGYNGSRGDPGIPGERGALGPRGPPGLPGEKGEKGNSVFILGATKGMQGDRGDPGPPGSPGSRGVRGPAGPAGYPGEPGLAGPPGPPGSPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVQPPDSGLYKGEKGIKGMPGMTGPPGPLGPRGEPGIGRKGEKGIPGFPGPRGDPGSYGSPGFPGLKGEPGLFGDPGSFGFLGPKGDPGDRGHPGPPGVLVTPSLPLKGPPGDPGHPGRYGETGAVGPRGPPGAPGRPGEACPGMTGPPGPRGFPGHPGFPGAAGVPGRADSAPGKPGNQGPTGLPGVPGLPGPPGSDVVYCTVGQPGPQGIKGKVGPPGRRGSKGEKGSSGLCACQPGPMGPSGPPGPPGRQGSKGDLGLPGWLGEKGSPGPPGAKGSPGPPGKPGASGLPGSKGEKGDMVVSRVKGHKGERGPDGPPGLPGQQGQHGRDGYAGEKGDTGPPGDHEDAAPGDKGPPGPPGPPGRAGPRGPPGLGFPGPPGDRGQPGAPGCPGNRGPDGRKGRKGDTIPCNVTYPGRPGPPGFDGPPGPKGLPGHRGAPGLRCLDGQKGQRGKPGISERPGPPGFRGDIGEPGLGGEKGSSPVGPPGAPGSPGVNGQKGHPGDPAIGYPGLPGKSGLPGAPGSKGLRGDAGRPGTTGPAGMPGFPGLQGPKGREGNAGFPGIPGLPGHSCERGAPGIPGQPGLPGAPGSPGAPGWKGQRGDMGPLGPAGMKGLPGVPGWPGADGTPGLPGVPGPTGDDGLPGLPGPKGPQGLPGFLGFPGERGKPGQDGHPGRKGEHGEKGWPGFLGDRGLKGVKGARGPPGYEGEMDIISKKGETGEPGASGDGGFPGENGDKGHPGMQGRRGEPGSHGPPGFHPGEPGRNGQPGLPGPPGPPGSPGLRGNIGFPGFPGDQGEPGSPGCPGPSGMDGTRGPKGYKGESASQFGLPGPKGKPGGPGYPGRLGASGEQGLPGAQGPGGPPGRPGPPGLFGPTGCPGSPGGPGLQGPPGEAGDPGPRGVLGDPGAPGLPGIKGPSGSPGLNGLHGLKGQKGSKGASGSHEMGPPGPVGMPGPKGETGDPGNPGISPPGLFGERGAPGPPGPPGRAGLPGPPGAPGKALKGDIPDTGLPGDQGPPGPDGPRGVPGPPGPPGSVDLLKGEPGDCGLPGPPGLPGPPGPPGHKGFPGCDGKHGQKGPMGFPGPQGPPGSPGPPGDKGLPGPPGRRGPLGPPGSRGEPGPPADLDACPRIPGLPGVPGPRGPEGTMGLPGMRGPPGPGCKGEPGLDGRRGEDGLPGSPGPPGHKGDMGEAGCPGAPGPPGPMGDPGPIGFGPGYLSGFLLVLHSQTDGEPTCPMGMPRLWTGYSLLYLEGQERAHNQDLGLAGSCLPIFSTLPFAYCNIHQVCHYARRNDRSYWLASTAPLPMTPLSEDEIRPYISRCAVCEAPAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVRPDLQFSSAPLPDTLKESHAQRQKISRCQVCVKHS

Target ORF information:

RefSeq Version	XM_006047131.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047131.1

ORF Insert Sequence:

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_006047129.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047191.1 Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA.

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CloneID	OBu05808
Clone ID Related Accession (Same CDS sequence)	XM_006047130.1 , XM_006047127.2 , XM_006047128.1 , XM_006047128.2 , XM_006047130.2 , XM_006047129.1 , XM_006047131.1 , XM_006047129.2 , XM_006047127.1 , XM_006047131.2
Accession Version	XM_006047129.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006047129.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047191.1
CDS	273..5342
Translation	MALVRCPFRCNKLLATNSWSLILILFSVQYAYGGGKKFIGLCGGRDCSLCQCFPEKGSRGQPGPPGPQGPIGPLGPPGPIGIPGEKGMKGDSGRPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGSRGRPGIDGYNGSRGDPGIPGERGALGPRGPPGLPGEKGEKGNSVFILGATKGMQGDRGDPGPPGSPGSRGVRGPAGPAGYPGEPGLAGPPGPPGSPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVQPPDSGLYKGEKGIKGMPGMTGPPGPLGPRGEPGIGRKGEKGIPGFPGPRGDPGSYGSPGFPGLKGEPGLFGDPGSFGFLGPKGDPGDRGHPGPPGVLVTPSLPLKGPPGDPGHPGRYGETGAVGPRGPPGAPGRPGEACPGMTGPPGPRGFPGHPGFPGAAGVPGRADSAPGKPGNQGPTGLPGVPGLPGPPGSDVVYCTVGQPGPQGIKGKVGPPGRRGSKGEKGSSGLCACQPGPMGPSGPPGPPGRQGSKGDLGLPGWLGEKGSPGPPGAKGSPGPPGKPGASGLPGSKGEKGDMVVSRVKGHKGERGPDGPPGLPGQQGQHGRDGYAGEKGDTGPPGDHEDAAPGDKGPPGPPGPPGRAGPRGPPGLGFPGPPGDRGQPGAPGCPGNRGPDGRKGRKGDTIPCNVTYPGRPGPPGFDGPPGPKGLPGHRGAPGLRCLDGQKGQRGKPGISERPGPPGFRGDIGEPGLGGEKGSSPVGPPGAPGSPGVNGQKGHPGDPAIGYPGLPGKSGLPGAPGSKGLRGDAGRPGTTGPAGMPGFPGLQGPKGREGNAGFPGIPGLPGHSCERGAPGIPGQPGLPGAPGSPGAPGWKGQRGDMGPLGPAGMKGLPGVPGWPGADGTPGLPGVPGPTGDDGLPGLPGPKGPQGLPGFLGFPGERGKPGQDGHPGRKGEHGEKGWPGFLGDRGLKGVKGARGPPGYEGEMDIISKKGETGEPGASGDGGFPGENGDKGHPGMQGRRGEPGSHGPPGFHPGEPGRNGQPGLPGPPGPPGSPGLRGNIGFPGFPGDQGEPGSPGCPGPSGMDGTRGPKGYKGESASQFGLPGPKGKPGGPGYPGRLGASGEQGLPGAQGPGGPPGRPGPPGLFGPTGCPGSPGGPGLQGPPGEAGDPGPRGVLGDPGAPGLPGIKGPSGSPGLNGLHGLKGQKGSKGASGSHEMGPPGPVGMPGPKGETGDPGNPGISPPGLFGERGAPGPPGPPGRAGLPGPPGAPGKALKGDIPDTGLPGDQGPPGPDGPRGVPGPPGPPGSVDLLKGEPGDCGLPGPPGLPGPPGPPGHKGFPGCDGKHGQKGPMGFPGPQGPPGSPGPPGDKGLPGPPGRRGPLGPPGSRGEPGPPADLDACPRIPGLPGVPGPRGPEGTMGLPGMRGPPGPGCKGEPGLDGRRGEDGLPGSPGPPGHKGDMGEAGCPGAPGPPGPMGDPGPIGFGPGYLSGFLLVLHSQTDGEPTCPMGMPRLWTGYSLLYLEGQERAHNQDLGLAGSCLPIFSTLPFAYCNIHQVCHYARRNDRSYWLASTAPLPMTPLSEDEIRPYISRCAVCEAPAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVRPDLQFSSAPLPDTLKESHAQRQKISRCQVCVKHS

Target ORF information:

RefSeq Version	XM_006047129.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047129.2

ORF Insert Sequence:

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_006047127.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047189.1 Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu05808
Clone ID Related Accession (Same CDS sequence)	XM_006047130.1 , XM_006047127.2 , XM_006047128.1 , XM_006047128.2 , XM_006047130.2 , XM_006047129.1 , XM_006047131.1 , XM_006047129.2 , XM_006047127.1 , XM_006047131.2
Accession Version	XM_006047127.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047189.1
CDS	266..5335
Translation	MALVRCPFRCNKLLATNSWSLILILFSVQYAYGGGKKFIGLCGGRDCSLCQCFPEKGSRGQPGPPGPQGPIGPLGPPGPIGIPGEKGMKGDSGRPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGSRGRPGIDGYNGSRGDPGIPGERGALGPRGPPGLPGEKGEKGNSVFILGATKGMQGDRGDPGPPGSPGSRGVRGPAGPAGYPGEPGLAGPPGPPGSPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVQPPDSGLYKGEKGIKGMPGMTGPPGPLGPRGEPGIGRKGEKGIPGFPGPRGDPGSYGSPGFPGLKGEPGLFGDPGSFGFLGPKGDPGDRGHPGPPGVLVTPSLPLKGPPGDPGHPGRYGETGAVGPRGPPGAPGRPGEACPGMTGPPGPRGFPGHPGFPGAAGVPGRADSAPGKPGNQGPTGLPGVPGLPGPPGSDVVYCTVGQPGPQGIKGKVGPPGRRGSKGEKGSSGLCACQPGPMGPSGPPGPPGRQGSKGDLGLPGWLGEKGSPGPPGAKGSPGPPGKPGASGLPGSKGEKGDMVVSRVKGHKGERGPDGPPGLPGQQGQHGRDGYAGEKGDTGPPGDHEDAAPGDKGPPGPPGPPGRAGPRGPPGLGFPGPPGDRGQPGAPGCPGNRGPDGRKGRKGDTIPCNVTYPGRPGPPGFDGPPGPKGLPGHRGAPGLRCLDGQKGQRGKPGISERPGPPGFRGDIGEPGLGGEKGSSPVGPPGAPGSPGVNGQKGHPGDPAIGYPGLPGKSGLPGAPGSKGLRGDAGRPGTTGPAGMPGFPGLQGPKGREGNAGFPGIPGLPGHSCERGAPGIPGQPGLPGAPGSPGAPGWKGQRGDMGPLGPAGMKGLPGVPGWPGADGTPGLPGVPGPTGDDGLPGLPGPKGPQGLPGFLGFPGERGKPGQDGHPGRKGEHGEKGWPGFLGDRGLKGVKGARGPPGYEGEMDIISKKGETGEPGASGDGGFPGENGDKGHPGMQGRRGEPGSHGPPGFHPGEPGRNGQPGLPGPPGPPGSPGLRGNIGFPGFPGDQGEPGSPGCPGPSGMDGTRGPKGYKGESASQFGLPGPKGKPGGPGYPGRLGASGEQGLPGAQGPGGPPGRPGPPGLFGPTGCPGSPGGPGLQGPPGEAGDPGPRGVLGDPGAPGLPGIKGPSGSPGLNGLHGLKGQKGSKGASGSHEMGPPGPVGMPGPKGETGDPGNPGISPPGLFGERGAPGPPGPPGRAGLPGPPGAPGKALKGDIPDTGLPGDQGPPGPDGPRGVPGPPGPPGSVDLLKGEPGDCGLPGPPGLPGPPGPPGHKGFPGCDGKHGQKGPMGFPGPQGPPGSPGPPGDKGLPGPPGRRGPLGPPGSRGEPGPPADLDACPRIPGLPGVPGPRGPEGTMGLPGMRGPPGPGCKGEPGLDGRRGEDGLPGSPGPPGHKGDMGEAGCPGAPGPPGPMGDPGPIGFGPGYLSGFLLVLHSQTDGEPTCPMGMPRLWTGYSLLYLEGQERAHNQDLGLAGSCLPIFSTLPFAYCNIHQVCHYARRNDRSYWLASTAPLPMTPLSEDEIRPYISRCAVCEAPAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVRPDLQFSSAPLPDTLKESHAQRQKISRCQVCVKHS

Target ORF information:

RefSeq Version	XM_006047127.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047127.1

ORF Insert Sequence:

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_006047131.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047193.1 Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu05808
Clone ID Related Accession (Same CDS sequence)	XM_006047130.1 , XM_006047127.2 , XM_006047128.1 , XM_006047128.2 , XM_006047130.2 , XM_006047129.1 , XM_006047131.1 , XM_006047129.2 , XM_006047127.1 , XM_006047131.2
Accession Version	XM_006047131.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006047131.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047193.1
CDS	250..5319
Translation	MALVRCPFRCNKLLATNSWSLILILFSVQYAYGGGKKFIGLCGGRDCSLCQCFPEKGSRGQPGPPGPQGPIGPLGPPGPIGIPGEKGMKGDSGRPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGSRGRPGIDGYNGSRGDPGIPGERGALGPRGPPGLPGEKGEKGNSVFILGATKGMQGDRGDPGPPGSPGSRGVRGPAGPAGYPGEPGLAGPPGPPGSPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVQPPDSGLYKGEKGIKGMPGMTGPPGPLGPRGEPGIGRKGEKGIPGFPGPRGDPGSYGSPGFPGLKGEPGLFGDPGSFGFLGPKGDPGDRGHPGPPGVLVTPSLPLKGPPGDPGHPGRYGETGAVGPRGPPGAPGRPGEACPGMTGPPGPRGFPGHPGFPGAAGVPGRADSAPGKPGNQGPTGLPGVPGLPGPPGSDVVYCTVGQPGPQGIKGKVGPPGRRGSKGEKGSSGLCACQPGPMGPSGPPGPPGRQGSKGDLGLPGWLGEKGSPGPPGAKGSPGPPGKPGASGLPGSKGEKGDMVVSRVKGHKGERGPDGPPGLPGQQGQHGRDGYAGEKGDTGPPGDHEDAAPGDKGPPGPPGPPGRAGPRGPPGLGFPGPPGDRGQPGAPGCPGNRGPDGRKGRKGDTIPCNVTYPGRPGPPGFDGPPGPKGLPGHRGAPGLRCLDGQKGQRGKPGISERPGPPGFRGDIGEPGLGGEKGSSPVGPPGAPGSPGVNGQKGHPGDPAIGYPGLPGKSGLPGAPGSKGLRGDAGRPGTTGPAGMPGFPGLQGPKGREGNAGFPGIPGLPGHSCERGAPGIPGQPGLPGAPGSPGAPGWKGQRGDMGPLGPAGMKGLPGVPGWPGADGTPGLPGVPGPTGDDGLPGLPGPKGPQGLPGFLGFPGERGKPGQDGHPGRKGEHGEKGWPGFLGDRGLKGVKGARGPPGYEGEMDIISKKGETGEPGASGDGGFPGENGDKGHPGMQGRRGEPGSHGPPGFHPGEPGRNGQPGLPGPPGPPGSPGLRGNIGFPGFPGDQGEPGSPGCPGPSGMDGTRGPKGYKGESASQFGLPGPKGKPGGPGYPGRLGASGEQGLPGAQGPGGPPGRPGPPGLFGPTGCPGSPGGPGLQGPPGEAGDPGPRGVLGDPGAPGLPGIKGPSGSPGLNGLHGLKGQKGSKGASGSHEMGPPGPVGMPGPKGETGDPGNPGISPPGLFGERGAPGPPGPPGRAGLPGPPGAPGKALKGDIPDTGLPGDQGPPGPDGPRGVPGPPGPPGSVDLLKGEPGDCGLPGPPGLPGPPGPPGHKGFPGCDGKHGQKGPMGFPGPQGPPGSPGPPGDKGLPGPPGRRGPLGPPGSRGEPGPPADLDACPRIPGLPGVPGPRGPEGTMGLPGMRGPPGPGCKGEPGLDGRRGEDGLPGSPGPPGHKGDMGEAGCPGAPGPPGPMGDPGPIGFGPGYLSGFLLVLHSQTDGEPTCPMGMPRLWTGYSLLYLEGQERAHNQDLGLAGSCLPIFSTLPFAYCNIHQVCHYARRNDRSYWLASTAPLPMTPLSEDEIRPYISRCAVCEAPAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVRPDLQFSSAPLPDTLKESHAQRQKISRCQVCVKHS

Target ORF information:

RefSeq Version	XM_006047131.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047131.2

ORF Insert Sequence:

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_006047132.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047194.1 Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X6, mRNA.

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CloneID	OBu05809
Clone ID Related Accession (Same CDS sequence)	XM_006047132.1
Accession Version	XM_006047132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-4(IV) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGCAACT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047194.1
CDS	267..5336
Translation	MALVRCPFRCNKLLATNSWSLILILFSVQYAYGGGKKFIGLCGGRDCSLCQCFPEKGSRGQPGPPGPQGPIGPLGPPGPIGIPGEKGMKGDSGRPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGSRGRPGIDGYNGSRGDPGIPGERGALGPRGPPGLPGEKGEKGNSVFILGATKGMQGDRGDPGPPGSPGSRGVRGPAGPAGYPGEPGLAGPPGPPGSPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVQPPDSGLYKGEKGIKGMPGMTGPPGPLGPRGEPGIGRKGEKGIPGFPGPRGDPGSYGSPGFPGLKGEPGLFGDPGSFGFLGPKGDPGDRGHPGPPGVLVTPSLPLKGPPGDPGHPGRYGETGAVGPRGPPGAPGRPGEACPGMTGPPGPRGFPGHPGFPGAAGVPGRADSAPGKPGNQGPTGLPGVPGLPGPPGSDVVYCTVGQPGPQGIKGKVGPPGRRGSKGEKGNSGLCACQPGPMGPSGPPGPPGRQGSKGDLGLPGWLGEKGSPGPPGAKGSPGPPGKPGASGLPGSKGEKGDMVVSRVKGHKGERGPDGPPGLPGQQGQHGRDGYAGEKGDTGPPGDHEDAAPGDKGPPGPPGPPGRAGPRGPPGLGFPGPPGDRGQPGAPGCPGNRGPDGRKGRKGDTIPCNVTYPGRPGPPGFDGPPGPKGLPGHRGAPGLRCLDGQKGQRGKPGISERPGPPGFRGDIGEPGLGGEKGSSPVGPPGAPGSPGVNGQKGHPGDPAIGYPGLPGKSGLPGAPGSKGLRGDAGRPGTTGPAGMPGFPGLQGPKGREGNAGFPGIPGLPGHSCERGAPGIPGQPGLPGAPGSPGAPGWKGQRGDMGPLGPAGMKGLPGVPGWPGADGTPGLPGVPGPTGDDGLPGLPGPKGPQGLPGFLGFPGERGKPGQDGHPGRKGEHGEKGWPGFLGDRGLKGVKGARGPPGYEGEMDIISKKGETGEPGASGDGGFPGENGDKGHPGMQGRRGEPGSHGPPGFHPGEPGRNGQPGLPGPPGPPGSPGLRGNIGFPGFPGDQGEPGSPGCPGPSGMDGTRGPKGYKGESASQFGLPGPKGKPGGPGYPGRLGASGEQGLPGAQGPGGPPGRPGPPGLFGPTGCPGSPGGPGLQGPPGEAGDPGPRGVLGDPGAPGLPGIKGPSGSPGLNGLHGLKGQKGSKGASGSHEMGPPGPVGMPGPKGETGDPGNPGISPPGLFGERGAPGPPGPPGRAGLPGPPGAPGKALKGDIPDTGLPGDQGPPGPDGPRGVPGPPGPPGSVDLLKGEPGDCGLPGPPGLPGPPGPPGHKGFPGCDGKHGQKGPMGFPGPQGPPGSPGPPGDKGLPGPPGRRGPLGPPGSRGEPGPPADLDACPRIPGLPGVPGPRGPEGTMGLPGMRGPPGPGCKGEPGLDGRRGEDGLPGSPGPPGHKGDMGEAGCPGAPGPPGPMGDPGPIGFGPGYLSGFLLVLHSQTDGEPTCPMGMPRLWTGYSLLYLEGQERAHNQDLGLAGSCLPIFSTLPFAYCNIHQVCHYARRNDRSYWLASTAPLPMTPLSEDEIRPYISRCAVCEAPAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVRPDLQFSSAPLPDTLKESHAQRQKISRCQVCVKHS

Target ORF information:

RefSeq Version	XM_006047132.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047132.1

ORF Insert Sequence:

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGCAACT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGATGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC 
CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA 
GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCGGGA 
TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC 
ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTTTGCTAT ACCTGGAAGG ACAGGAGAGA 
GCTCACAATC AAGACCTAGG TCTGGCGGGC TCCTGCCTGC CCATCTTTAG CACGCTGCCC 
TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC 
TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCACTCT CGGAGGATGA GATCCGCCCG 
TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA 
GACCAGTCCA TCCCCCCGTG TCCGCGGACC TGGAGGAGCC TCTGGATCGG GTACTCCTTC 
CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC 
TGCCTAGAGG ATTTCAGAGC TGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC 
CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT 
TCCTCTGCAC CGTTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC 
AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_006047133.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047195.1 Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X7, mRNA.

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CloneID	OBu05810
Clone ID Related Accession (Same CDS sequence)	XM_006047133.1
Accession Version	XM_006047133.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-4(IV) chain isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTGCCCCA GGAAAAGCTC TTAAGGGTGA CATTCCAGAC 
ACGGGTCTGC CTGGAGATCA GGGGCCTCCT GGCCCCGATG GTCCAAGAGG AGTACCCGGG 
CCTCCAGGAC CACCTGGGAG TGTCGACCTT CTGAAAGGAG AACCAGGAGA CTGTGGTCTG 
CCGGGGCCTC CAGGTCTCCC AGGCCCACCA GGCCCTCCAG GACACAAGGG CTTCCCAGGA 
TGTGATGGAA AACATGGCCA GAAAGGACCA ATGGGATTCC CGGGGCCGCA GGGGCCACCT 
GGAAGTCCTG GGCCACCTGG AGACAAAGGT TTACCTGGAC CTCCAGGCAG ACGAGGGCCT 
TTGGGTCCTC CGGGTTCCAG AGGTGAGCCT GGGCCCCCAG CAGACTTGGA TGCATGCCCC 
CGAATCCCAG GGCTTCCTGG GGTACCAGGC CCGAGAGGAC CGGAAGGAAC CATGGGGCTT 
CCTGGCATGA GAGGCCCGCC GGGACCAGGA TGCAAAGGAG AGCCTGGGCT GGATGGCAGG 
AGGGGTGAGG ATGGCCTCCC AGGGTCCCCT GGGCCTCCGG GACATAAAGG TGACATGGGA 
GAAGCAGGCT GTCCAGGAGC ACCAGGCCCT CCTGGGCCCA TGGGGGATCC TGGGCCCATA 
GGGTTTGGCC CGGGATACCT CAGTGGCTTC CTCCTGGTTC TCCACAGTCA GACGGATGGA 
GAACCCACCT GCCCCATGGG CATGCCCAGG CTCTGGACTG GGTATAGTTT GCTATACCTG 
GAAGGACAGG AGAGAGCTCA CAATCAAGAC CTAGGTCTGG CGGGCTCCTG CCTGCCCATC 
TTTAGCACGC TGCCCTTCGC CTACTGCAAC ATCCACCAGG TGTGCCACTA TGCCCGGAGG 
AACGACAGGT CCTACTGGCT GGCGAGCACA GCGCCTCTGC CCATGACTCC ACTCTCGGAG 
GATGAGATCC GCCCGTACAT CAGCCGCTGC GCAGTGTGCG AGGCCCCCGC CCAGGCCGTG 
GCGGTGCACA GCCAAGACCA GTCCATCCCC CCGTGTCCGC GGACCTGGAG GAGCCTCTGG 
ATCGGGTACT CCTTCCTCAT GCACACAGGC GCTGGGGACC AAGGAGGCGG GCAGGCCCTC 
ATGTCCCCAG GCAGCTGCCT AGAGGATTTC AGAGCTGCAC CGTTCCTGGA ATGCCAAGGC 
CGGCAGGGAA CTTGCCACTT TTTTGCCAAC AAGTATAGTT TCTGGCTGAC AACAGTGAGA 
CCTGACTTGC AGTTTTCCTC TGCACCGTTA CCGGACACCT TGAAAGAAAG CCATGCTCAA 
CGCCAGAAAA TCAGCAGGTG CCAGGTCTGC GTGAAGCATA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047195.1
CDS	266..5170
Translation	MALVRCPFRCNKLLATNSWSLILILFSVQYAYGGGKKFIGLCGGRDCSLCQCFPEKGSRGQPGPPGPQGPIGPLGPPGPIGIPGEKGMKGDSGRPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGSRGRPGIDGYNGSRGDPGIPGERGALGPRGPPGLPGEKGEKGNSVFILGATKGMQGDRGDPGPPGSPGSRGVRGPAGPAGYPGEPGLAGPPGPPGSPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVQPPDSGLYKGEKGIKGMPGMTGPPGPLGPRGEPGIGRKGEKGIPGFPGPRGDPGSYGSPGFPGLKGEPGLFGDPGSFGFLGPKGDPGDRGHPGPPGVLVTPSLPLKGPPGDPGHPGRYGETGAVGPRGPPGAPGRPGEACPGMTGPPGPRGFPGHPGFPGAAGVPGRADSAPGKPGNQGPTGLPGVPGLPGPPGSDVVYCTVGQPGPQGIKGKVGPPGRRGSKGEKGSSGLCACQPGPMGPSGPPGPPGRQGSKGDLGLPGWLGEKGSPGPPGAKGSPGPPGKPGASGLPGSKGEKGDMVVSRVKGHKGERGPDGPPGLPGQQGQHGRDGYAGEKGDTGPPGDHEDAAPGDKGPPGPPGPPGRAGPRGPPGLGFPGPPGDRGQPGAPGCPGNRGPDGRKGRKGDTIPCNVTYPGRPGPPGFDGPPGPKGLPGHRGAPGLRCLDGQKGQRGKPGISERPGPPGFRGDIGEPGLGGEKGSSPVGPPGAPGSPGVNGQKGHPGDPAIGYPGLPGKSGLPGAPGSKGLRGDAGRPGTTGPAGMPGFPGLQGPKGREGNAGFPGIPGLPGHSCERGAPGIPGQPGLPGAPGSPGAPGWKGQRGDMGPLGPAGMKGLPGVPGWPGADGTPGLPGVPGPTGDDGLPGLPGPKGPQGLPGFLGFPGERGKPGQDGHPGRKGEHGEKGWPGFLGDRGLKGVKGARGPPGYEGEMDIISKKGETGEPGASGDGGFPGENGDKGHPGMQGRRGEPGSHGPPGFHPGEPGRNGQPGLPGPPGPPGSPGLRGNIGFPGFPGDQGEPGSPGCPGPSGMDGTRGPKGYKGESASQFGLPGPKGKPGGPGYPGRLGASGEQGLPGAQGPGGPPGRPGPPGLFGPTGCPGSPGGPGLQGPPGEAGDPGPRGVLGDPGAPGLPGIKGPSGSPGLNGLHGLKGQKGSKGASGAPGKALKGDIPDTGLPGDQGPPGPDGPRGVPGPPGPPGSVDLLKGEPGDCGLPGPPGLPGPPGPPGHKGFPGCDGKHGQKGPMGFPGPQGPPGSPGPPGDKGLPGPPGRRGPLGPPGSRGEPGPPADLDACPRIPGLPGVPGPRGPEGTMGLPGMRGPPGPGCKGEPGLDGRRGEDGLPGSPGPPGHKGDMGEAGCPGAPGPPGPMGDPGPIGFGPGYLSGFLLVLHSQTDGEPTCPMGMPRLWTGYSLLYLEGQERAHNQDLGLAGSCLPIFSTLPFAYCNIHQVCHYARRNDRSYWLASTAPLPMTPLSEDEIRPYISRCAVCEAPAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVRPDLQFSSAPLPDTLKESHAQRQKISRCQVCVKHS

Target ORF information:

RefSeq Version	XM_006047133.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047133.1

ORF Insert Sequence:

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTGCCCCA GGAAAAGCTC TTAAGGGTGA CATTCCAGAC 
ACGGGTCTGC CTGGAGATCA GGGGCCTCCT GGCCCCGATG GTCCAAGAGG AGTACCCGGG 
CCTCCAGGAC CACCTGGGAG TGTCGACCTT CTGAAAGGAG AACCAGGAGA CTGTGGTCTG 
CCGGGGCCTC CAGGTCTCCC AGGCCCACCA GGCCCTCCAG GACACAAGGG CTTCCCAGGA 
TGTGATGGAA AACATGGCCA GAAAGGACCA ATGGGATTCC CGGGGCCGCA GGGGCCACCT 
GGAAGTCCTG GGCCACCTGG AGACAAAGGT TTACCTGGAC CTCCAGGCAG ACGAGGGCCT 
TTGGGTCCTC CGGGTTCCAG AGGTGAGCCT GGGCCCCCAG CAGACTTGGA TGCATGCCCC 
CGAATCCCAG GGCTTCCTGG GGTACCAGGC CCGAGAGGAC CGGAAGGAAC CATGGGGCTT 
CCTGGCATGA GAGGCCCGCC GGGACCAGGA TGCAAAGGAG AGCCTGGGCT GGATGGCAGG 
AGGGGTGAGG ATGGCCTCCC AGGGTCCCCT GGGCCTCCGG GACATAAAGG TGACATGGGA 
GAAGCAGGCT GTCCAGGAGC ACCAGGCCCT CCTGGGCCCA TGGGGGATCC TGGGCCCATA 
GGGTTTGGCC CGGGATACCT CAGTGGCTTC CTCCTGGTTC TCCACAGTCA GACGGATGGA 
GAACCCACCT GCCCCATGGG CATGCCCAGG CTCTGGACTG GGTATAGTTT GCTATACCTG 
GAAGGACAGG AGAGAGCTCA CAATCAAGAC CTAGGTCTGG CGGGCTCCTG CCTGCCCATC 
TTTAGCACGC TGCCCTTCGC CTACTGCAAC ATCCACCAGG TGTGCCACTA TGCCCGGAGG 
AACGACAGGT CCTACTGGCT GGCGAGCACA GCGCCTCTGC CCATGACTCC ACTCTCGGAG 
GATGAGATCC GCCCGTACAT CAGCCGCTGC GCAGTGTGCG AGGCCCCCGC CCAGGCCGTG 
GCGGTGCACA GCCAAGACCA GTCCATCCCC CCGTGTCCGC GGACCTGGAG GAGCCTCTGG 
ATCGGGTACT CCTTCCTCAT GCACACAGGC GCTGGGGACC AAGGAGGCGG GCAGGCCCTC 
ATGTCCCCAG GCAGCTGCCT AGAGGATTTC AGAGCTGCAC CGTTCCTGGA ATGCCAAGGC 
CGGCAGGGAA CTTGCCACTT TTTTGCCAAC AAGTATAGTT TCTGGCTGAC AACAGTGAGA 
CCTGACTTGC AGTTTTCCTC TGCACCGTTA CCGGACACCT TGAAAGAAAG CCATGCTCAA 
CGCCAGAAAA TCAGCAGGTG CCAGGTCTGC GTGAAGCATA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_006047134.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047196.1 Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X8, mRNA.

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CloneID	OBu05811
Clone ID Related Accession (Same CDS sequence)	XM_006047134.1
Accession Version	XM_006047134.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-4(IV) chain isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTGCCCCA GGAAAAGCTC TTAAGGGTGA CATTCCAGAC 
ACGGGTCTGC CTGGAGATCA GGGGCCTCCT GGCCCCGATG GTCCAAGAGG AGTACCCGGG 
CCTCCAGGAC CACCTGGGAG TGTCGACCTT CTGAAAGGAG AACCAGGAGA CTGTGGTCTG 
CCGGGGCCTC CAGGTCTCCC AGGCCCACCA GGCCCTCCAG GACACAAGGG CTTCCCAGGA 
TGTGATGGAA AACATGGCCA GAAAGGACCA ATGGGATTCC CGGGGCCGCA GGGGCCACCT 
GGAAGTCCTG GGCCACCTGG AGACAAAGGT TTACCTGGAC CTCCAGGCAG ACGAGGGCCT 
TTGGGTCCTC CGGGTTCCAG AGGTGAGCCT GGGCCCCCAG CAGACTTGGA TGCATGCCCC 
CGAATCCCAG GGCTTCCTGG GGTACCAGGC CCGAGAGGAC CGGAAGGAAC CATGGGGCTT 
CCTGGCATGA GAGGCCCGCC GGGACCAGGA TGCAAAGGAG AGCCTGGGCT GGATGGCAGG 
AGGGGTGAGG ATGGCCTCCC AGGGTCCCCT GGGCCTCCGG GACATAAAGG TGACATGGGA 
GAAGCAGGCT GTCCAGGAGC ACCAGGCCCT CCTGGGCCCA TGGGGGATCC TGGGCCCATA 
GGGTTTGGCC CGGGATACCT CAGTGGCTTC CTCCTGGTTC TCCACAGTCA GACGGATGGA 
GAACCCACCT GCCCCATGGG CATGCCCAGG CTCTGGACTG GGTATAGTTT GCTATACCTG 
GAAGGACAGG AGAGAGCTCA CAATCAAGAC CTAGGTCTGG CGGGCTCCTG CCTGCCCATC 
TTTAGCACGC TGCCCTTCGC CTACTGCAAC ATCCACCAGG TGTGCCACTA TGCCCGGAGG 
AACGACAGGT CCTACTGGCT GGCGAGCACA GCGCCTCTGC CCATGACTCC ACTCTCGGAG 
GATGAGATCC GCCCGTACAT CAGCCGCTGC GCAGTGTGCG AGGCCCCCGC CCAGGCCGTG 
GCGGTGCACA GCCAAGACCA GTCCATCCCC CCGTGTCCGC GGACCTGGAG GAGCCTCTGG 
ATCGGGTACT CCTTCCTCAT GCACACAGGC GCTGGGGACC AAGGAGGCGG GCAGGCCCTC 
ATGTCCCCAG GCAGCTGCCT AGAGGATTTC AGAGCTGCAC CGTTCCTGGA ATGCCAAGGC 
CGGCAGGGAA CTTGCCACTT TTTTGCCAAC AAGTATAGTT TCTGGCTGAC AACAGTGAGA 
CCTGACTTGC AGTTTTCCTC TGCACCGTTA CCGGACACCT TGAAAGAAAG CCATGCTCAA 
CGCCAGAAAA TCAGCAGGTG CCAGGTCTGC GTGAAGCATA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047196.1
CDS	266..4990
Translation	MALVRCPFRCNKLLATNSWSLILILFSVQYAYGGGKKFIGLCGGRDCSLCQCFPEKGSRGQPGPPGPQGPIGPLGPPGPIGIPGEKGMKGDSGRPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGSRGRPGIDGYNGSRGDPGIPGERGALGPRGPPGLPGEKGEKGNSVFILGATKGMQGDRGDPGPPGSPGSRGVRGPAGPAGYPGEPGLAGPPGPPGSPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVQPPDSGLYKGEKGIKGMPGMTGPPGPLGPRGEPGIGRKGEKGIPGFPGPRGDPGSYGSPGFPGLKGEPGLFGDPGSFGFLGPKGDPGDRGHPGPPGVLVTPSLPLKGPPGDPGHPGRYGETGAVGPRGPPGAPGRPGEACPGMTGPPGPRGFPGHPGFPGAAGVPGRADSAPGKPGNQGPTGLPGVPGLPGPPGSDVVYCTVGQPGPQGIKGKVGPPGRRGSKGEKGSSGLCACQPGPMGPSGPPGPPGRQGSKGDLGLPGWLGEKGSPGPPGAKGSPGPPGKPGASGLPGSKGEKGDMVVSRVKGHKGERGPDGPPGLPGQQGQHGRDGYAGEKGDTGPPGDHEDAAPGDKGPPGPPGPPGRAGPRGPPGLGFPGPPGDRGQPGAPGCPGNRGPDGRKGRKGDTIPCNVTYPGRPGPPGFDGPPGPKGLPGHRGAPGLRCLDGQKGQRGKPGISERPGPPGFRGDIGEPGLGGEKGSSPVGPPGAPGSPGVNGQKGHPGDPAIGYPGLPGKSGLPGAPGSKGLRGDAGRPGTTGPAGMPGFPGLQGPKGREGNAGFPGIPGLPGHSCERGAPGIPGQPGLPGAPGSPGAPGWKGQRGDMGPLGPAGMKGLPGVPGWPGADGTPGLPGVPGPTGDDGLPGLPGPKGPQGLPGFLGFPGERGKPGQDGHPGRKGEHGEKGWPGFLGDRGLKGVKGARGPPGYEGEMDIISKKGETGEPGASGDGGFPGENGDKGHPGMQGRRGEPGSHGPPGFHPGEPGRNGQPGLPGPPGPPGSPGLRGNIGFPGFPGDQGEPGSPGCPGPSGMDGTRGPKGYKGESASQFGLPGPKGKPGGPGYPGRLGASGEQGLPGAQGPGGPPGRPGPPGLFGPTGCPGAPGKALKGDIPDTGLPGDQGPPGPDGPRGVPGPPGPPGSVDLLKGEPGDCGLPGPPGLPGPPGPPGHKGFPGCDGKHGQKGPMGFPGPQGPPGSPGPPGDKGLPGPPGRRGPLGPPGSRGEPGPPADLDACPRIPGLPGVPGPRGPEGTMGLPGMRGPPGPGCKGEPGLDGRRGEDGLPGSPGPPGHKGDMGEAGCPGAPGPPGPMGDPGPIGFGPGYLSGFLLVLHSQTDGEPTCPMGMPRLWTGYSLLYLEGQERAHNQDLGLAGSCLPIFSTLPFAYCNIHQVCHYARRNDRSYWLASTAPLPMTPLSEDEIRPYISRCAVCEAPAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVRPDLQFSSAPLPDTLKESHAQRQKISRCQVCVKHS

Target ORF information:

RefSeq Version	XM_006047134.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047134.1

ORF Insert Sequence:

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTGCCCCA GGAAAAGCTC TTAAGGGTGA CATTCCAGAC 
ACGGGTCTGC CTGGAGATCA GGGGCCTCCT GGCCCCGATG GTCCAAGAGG AGTACCCGGG 
CCTCCAGGAC CACCTGGGAG TGTCGACCTT CTGAAAGGAG AACCAGGAGA CTGTGGTCTG 
CCGGGGCCTC CAGGTCTCCC AGGCCCACCA GGCCCTCCAG GACACAAGGG CTTCCCAGGA 
TGTGATGGAA AACATGGCCA GAAAGGACCA ATGGGATTCC CGGGGCCGCA GGGGCCACCT 
GGAAGTCCTG GGCCACCTGG AGACAAAGGT TTACCTGGAC CTCCAGGCAG ACGAGGGCCT 
TTGGGTCCTC CGGGTTCCAG AGGTGAGCCT GGGCCCCCAG CAGACTTGGA TGCATGCCCC 
CGAATCCCAG GGCTTCCTGG GGTACCAGGC CCGAGAGGAC CGGAAGGAAC CATGGGGCTT 
CCTGGCATGA GAGGCCCGCC GGGACCAGGA TGCAAAGGAG AGCCTGGGCT GGATGGCAGG 
AGGGGTGAGG ATGGCCTCCC AGGGTCCCCT GGGCCTCCGG GACATAAAGG TGACATGGGA 
GAAGCAGGCT GTCCAGGAGC ACCAGGCCCT CCTGGGCCCA TGGGGGATCC TGGGCCCATA 
GGGTTTGGCC CGGGATACCT CAGTGGCTTC CTCCTGGTTC TCCACAGTCA GACGGATGGA 
GAACCCACCT GCCCCATGGG CATGCCCAGG CTCTGGACTG GGTATAGTTT GCTATACCTG 
GAAGGACAGG AGAGAGCTCA CAATCAAGAC CTAGGTCTGG CGGGCTCCTG CCTGCCCATC 
TTTAGCACGC TGCCCTTCGC CTACTGCAAC ATCCACCAGG TGTGCCACTA TGCCCGGAGG 
AACGACAGGT CCTACTGGCT GGCGAGCACA GCGCCTCTGC CCATGACTCC ACTCTCGGAG 
GATGAGATCC GCCCGTACAT CAGCCGCTGC GCAGTGTGCG AGGCCCCCGC CCAGGCCGTG 
GCGGTGCACA GCCAAGACCA GTCCATCCCC CCGTGTCCGC GGACCTGGAG GAGCCTCTGG 
ATCGGGTACT CCTTCCTCAT GCACACAGGC GCTGGGGACC AAGGAGGCGG GCAGGCCCTC 
ATGTCCCCAG GCAGCTGCCT AGAGGATTTC AGAGCTGCAC CGTTCCTGGA ATGCCAAGGC 
CGGCAGGGAA CTTGCCACTT TTTTGCCAAC AAGTATAGTT TCTGGCTGAC AACAGTGAGA 
CCTGACTTGC AGTTTTCCTC TGCACCGTTA CCGGACACCT TGAAAGAAAG CCATGCTCAA 
CGCCAGAAAA TCAGCAGGTG CCAGGTCTGC GTGAAGCATA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_006047135.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047197.1 Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBu05812
Clone ID Related Accession (Same CDS sequence)	XM_006047135.2 , XM_006047135.1
Accession Version	XM_006047135.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-4(IV) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006047135.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGTATGAA CCTCACTCTG ATTTCTGGCA TTGAAGATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047197.1
CDS	248..4501
Translation	MALVRCPFRCNKLLATNSWSLILILFSVQYAYGGGKKFIGLCGGRDCSLCQCFPEKGSRGQPGPPGPQGPIGPLGPPGPIGIPGEKGMKGDSGRPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGSRGRPGIDGYNGSRGDPGIPGERGALGPRGPPGLPGEKGEKGNSVFILGATKGMQGDRGDPGPPGSPGSRGVRGPAGPAGYPGEPGLAGPPGPPGSPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVQPPDSGLYKGEKGIKGMPGMTGPPGPLGPRGEPGIGRKGEKGIPGFPGPRGDPGSYGSPGFPGLKGEPGLFGDPGSFGFLGPKGDPGDRGHPGPPGVLVTPSLPLKGPPGDPGHPGRYGETGAVGPRGPPGAPGRPGEACPGMTGPPGPRGFPGHPGFPGAAGVPGRADSAPGKPGNQGPTGLPGVPGLPGPPGSDVVYCTVGQPGPQGIKGKVGPPGRRGSKGEKGSSGLCACQPGPMGPSGPPGPPGRQGSKGDLGLPGWLGEKGSPGPPGAKGSPGPPGKPGASGLPGSKGEKGDMVVSRVKGHKGERGPDGPPGLPGQQGQHGRDGYAGEKGDTGPPGDHEDAAPGDKGPPGPPGPPGRAGPRGPPGLGFPGPPGDRGQPGAPGCPGNRGPDGRKGRKGDTIPCNVTYPGRPGPPGFDGPPGPKGLPGHRGAPGLRCLDGQKGQRGKPGISERPGPPGFRGDIGEPGLGGEKGSSPVGPPGAPGSPGVNGQKGHPGDPAIGYPGLPGKSGLPGAPGSKGLRGDAGRPGTTGPAGMPGFPGLQGPKGREGNAGFPGIPGLPGHSCERGAPGIPGQPGLPGAPGSPGAPGWKGQRGDMGPLGPAGMKGLPGVPGWPGADGTPGLPGVPGPTGDDGLPGLPGPKGPQGLPGFLGFPGERGKPGQDGHPGRKGEHGEKGWPGFLGDRGLKGVKGARGPPGYEGEMDIISKKGETGEPGASGDGGFPGENGDKGHPGMQGRRGEPGSHGPPGFHPGEPGRNGQPGLPGPPGPPGSPGLRGNIGFPGFPGDQGEPGSPGCPGPSGMDGTRGPKGYKGESASQFGLPGPKGKPGGPGYPGRLGASGEQGLPGAQGPGGPPGRPGPPGLFGPTGCPGSPGGPGLQGPPGEAGDPGPRGVLGDPGAPGLPGIKGPSGSPGLNGLHGLKGQKGSKGASGSHEMGPPGPVGMPGPKGETGDPGNPGISPPGLFGERGAPGPPGPPGRAGLPGPPGAPGKALKGDIPDTGLPGDQGPPGPDGPRGVPGPPGPPGSVDLLKGEPGDCGLPGPPGLPGPPGPPGHKGFPGCDGKHGQKGPMGFPGPQGPPGSPGPPGDKGLPGPPGRRGPLGPPGSRGEPGPPADLDACPRIPGLPGVPGPRGPEGTMGLPGMRGPPGPGMNLTLISGIEDC

Target ORF information:

RefSeq Version	XM_006047135.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047135.2

ORF Insert Sequence:

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGTATGAA CCTCACTCTG ATTTCTGGCA TTGAAGATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_006047135.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047197.1 Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBu05812
Clone ID Related Accession (Same CDS sequence)	XM_006047135.2 , XM_006047135.1
Accession Version	XM_006047135.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-4(IV) chain isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGTATGAA CCTCACTCTG ATTTCTGGCA TTGAAGATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047197.1
CDS	270..4523
Translation	MALVRCPFRCNKLLATNSWSLILILFSVQYAYGGGKKFIGLCGGRDCSLCQCFPEKGSRGQPGPPGPQGPIGPLGPPGPIGIPGEKGMKGDSGRPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGSRGRPGIDGYNGSRGDPGIPGERGALGPRGPPGLPGEKGEKGNSVFILGATKGMQGDRGDPGPPGSPGSRGVRGPAGPAGYPGEPGLAGPPGPPGSPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVQPPDSGLYKGEKGIKGMPGMTGPPGPLGPRGEPGIGRKGEKGIPGFPGPRGDPGSYGSPGFPGLKGEPGLFGDPGSFGFLGPKGDPGDRGHPGPPGVLVTPSLPLKGPPGDPGHPGRYGETGAVGPRGPPGAPGRPGEACPGMTGPPGPRGFPGHPGFPGAAGVPGRADSAPGKPGNQGPTGLPGVPGLPGPPGSDVVYCTVGQPGPQGIKGKVGPPGRRGSKGEKGSSGLCACQPGPMGPSGPPGPPGRQGSKGDLGLPGWLGEKGSPGPPGAKGSPGPPGKPGASGLPGSKGEKGDMVVSRVKGHKGERGPDGPPGLPGQQGQHGRDGYAGEKGDTGPPGDHEDAAPGDKGPPGPPGPPGRAGPRGPPGLGFPGPPGDRGQPGAPGCPGNRGPDGRKGRKGDTIPCNVTYPGRPGPPGFDGPPGPKGLPGHRGAPGLRCLDGQKGQRGKPGISERPGPPGFRGDIGEPGLGGEKGSSPVGPPGAPGSPGVNGQKGHPGDPAIGYPGLPGKSGLPGAPGSKGLRGDAGRPGTTGPAGMPGFPGLQGPKGREGNAGFPGIPGLPGHSCERGAPGIPGQPGLPGAPGSPGAPGWKGQRGDMGPLGPAGMKGLPGVPGWPGADGTPGLPGVPGPTGDDGLPGLPGPKGPQGLPGFLGFPGERGKPGQDGHPGRKGEHGEKGWPGFLGDRGLKGVKGARGPPGYEGEMDIISKKGETGEPGASGDGGFPGENGDKGHPGMQGRRGEPGSHGPPGFHPGEPGRNGQPGLPGPPGPPGSPGLRGNIGFPGFPGDQGEPGSPGCPGPSGMDGTRGPKGYKGESASQFGLPGPKGKPGGPGYPGRLGASGEQGLPGAQGPGGPPGRPGPPGLFGPTGCPGSPGGPGLQGPPGEAGDPGPRGVLGDPGAPGLPGIKGPSGSPGLNGLHGLKGQKGSKGASGSHEMGPPGPVGMPGPKGETGDPGNPGISPPGLFGERGAPGPPGPPGRAGLPGPPGAPGKALKGDIPDTGLPGDQGPPGPDGPRGVPGPPGPPGSVDLLKGEPGDCGLPGPPGLPGPPGPPGHKGFPGCDGKHGQKGPMGFPGPQGPPGSPGPPGDKGLPGPPGRRGPLGPPGSRGEPGPPADLDACPRIPGLPGVPGPRGPEGTMGLPGMRGPPGPGMNLTLISGIEDC

Target ORF information:

RefSeq Version	XM_006047135.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047135.1

ORF Insert Sequence:

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCAT GCCCCCGAAT CCCAGGGCTT 
CCTGGGGTAC CAGGCCCGAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC 
CCGCCGGGAC CAGGTATGAA CCTCACTCTG ATTTCTGGCA TTGAAGATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_006047136.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047198.1 Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X10, mRNA.

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CloneID	OBu05813
Clone ID Related Accession (Same CDS sequence)	XM_006047136.1 , XM_006047136.2
Accession Version	XM_006047136.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-4(IV) chain isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783978.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG GTGATTCCAA TTTCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047198.1
CDS	271..4380
Translation	MALVRCPFRCNKLLATNSWSLILILFSVQYAYGGGKKFIGLCGGRDCSLCQCFPEKGSRGQPGPPGPQGPIGPLGPPGPIGIPGEKGMKGDSGRPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGSRGRPGIDGYNGSRGDPGIPGERGALGPRGPPGLPGEKGEKGNSVFILGATKGMQGDRGDPGPPGSPGSRGVRGPAGPAGYPGEPGLAGPPGPPGSPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVQPPDSGLYKGEKGIKGMPGMTGPPGPLGPRGEPGIGRKGEKGIPGFPGPRGDPGSYGSPGFPGLKGEPGLFGDPGSFGFLGPKGDPGDRGHPGPPGVLVTPSLPLKGPPGDPGHPGRYGETGAVGPRGPPGAPGRPGEACPGMTGPPGPRGFPGHPGFPGAAGVPGRADSAPGKPGNQGPTGLPGVPGLPGPPGSDVVYCTVGQPGPQGIKGKVGPPGRRGSKGEKGSSGLCACQPGPMGPSGPPGPPGRQGSKGDLGLPGWLGEKGSPGPPGAKGSPGPPGKPGASGLPGSKGEKGDMVVSRVKGHKGERGPDGPPGLPGQQGQHGRDGYAGEKGDTGPPGDHEDAAPGDKGPPGPPGPPGRAGPRGPPGLGFPGPPGDRGQPGAPGCPGNRGPDGRKGRKGDTIPCNVTYPGRPGPPGFDGPPGPKGLPGHRGAPGLRCLDGQKGQRGKPGISERPGPPGFRGDIGEPGLGGEKGSSPVGPPGAPGSPGVNGQKGHPGDPAIGYPGLPGKSGLPGAPGSKGLRGDAGRPGTTGPAGMPGFPGLQGPKGREGNAGFPGIPGLPGHSCERGAPGIPGQPGLPGAPGSPGAPGWKGQRGDMGPLGPAGMKGLPGVPGWPGADGTPGLPGVPGPTGDDGLPGLPGPKGPQGLPGFLGFPGERGKPGQDGHPGRKGEHGEKGWPGFLGDRGLKGVKGARGPPGYEGEMDIISKKGETGEPGASGDGGFPGENGDKGHPGMQGRRGEPGSHGPPGFHPGEPGRNGQPGLPGPPGPPGSPGLRGNIGFPGFPGDQGEPGSPGCPGPSGMDGTRGPKGYKGESASQFGLPGPKGKPGGPGYPGRLGASGEQGLPGAQGPGGPPGRPGPPGLFGPTGCPGSPGGPGLQGPPGEAGDPGPRGVLGDPGAPGLPGIKGPSGSPGLNGLHGLKGQKGSKGASGSHEMGPPGPVGMPGPKGETGDPGNPGISPPGLFGERGAPGPPGPPGRAGLPGPPGAPGKALKGDIPDTGLPGDQGPPGPDGPRGVPGPPGPPGSVDLLKGEPGDCGLPGPPGLPGPPGPPGHKGFPGCDGKHGQKGPMGFPGPQGPPGSPGPPGDKGLPGPPGRRGPLGPPGSRGGDSNFR

Target ORF information:

RefSeq Version	XM_006047136.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen, type IV, alpha 4 (COL4A4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047136.1

ORF Insert Sequence:

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG GTGATTCCAA TTTCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_006047136.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047198.1 Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X8, mRNA.

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CloneID	OBu05813
Clone ID Related Accession (Same CDS sequence)	XM_006047136.1 , XM_006047136.2
Accession Version	XM_006047136.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	collagen alpha-4(IV) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006047136.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG GTGATTCCAA TTTCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047198.1
CDS	248..4357
Translation	MALVRCPFRCNKLLATNSWSLILILFSVQYAYGGGKKFIGLCGGRDCSLCQCFPEKGSRGQPGPPGPQGPIGPLGPPGPIGIPGEKGMKGDSGRPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGSRGRPGIDGYNGSRGDPGIPGERGALGPRGPPGLPGEKGEKGNSVFILGATKGMQGDRGDPGPPGSPGSRGVRGPAGPAGYPGEPGLAGPPGPPGSPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVQPPDSGLYKGEKGIKGMPGMTGPPGPLGPRGEPGIGRKGEKGIPGFPGPRGDPGSYGSPGFPGLKGEPGLFGDPGSFGFLGPKGDPGDRGHPGPPGVLVTPSLPLKGPPGDPGHPGRYGETGAVGPRGPPGAPGRPGEACPGMTGPPGPRGFPGHPGFPGAAGVPGRADSAPGKPGNQGPTGLPGVPGLPGPPGSDVVYCTVGQPGPQGIKGKVGPPGRRGSKGEKGSSGLCACQPGPMGPSGPPGPPGRQGSKGDLGLPGWLGEKGSPGPPGAKGSPGPPGKPGASGLPGSKGEKGDMVVSRVKGHKGERGPDGPPGLPGQQGQHGRDGYAGEKGDTGPPGDHEDAAPGDKGPPGPPGPPGRAGPRGPPGLGFPGPPGDRGQPGAPGCPGNRGPDGRKGRKGDTIPCNVTYPGRPGPPGFDGPPGPKGLPGHRGAPGLRCLDGQKGQRGKPGISERPGPPGFRGDIGEPGLGGEKGSSPVGPPGAPGSPGVNGQKGHPGDPAIGYPGLPGKSGLPGAPGSKGLRGDAGRPGTTGPAGMPGFPGLQGPKGREGNAGFPGIPGLPGHSCERGAPGIPGQPGLPGAPGSPGAPGWKGQRGDMGPLGPAGMKGLPGVPGWPGADGTPGLPGVPGPTGDDGLPGLPGPKGPQGLPGFLGFPGERGKPGQDGHPGRKGEHGEKGWPGFLGDRGLKGVKGARGPPGYEGEMDIISKKGETGEPGASGDGGFPGENGDKGHPGMQGRRGEPGSHGPPGFHPGEPGRNGQPGLPGPPGPPGSPGLRGNIGFPGFPGDQGEPGSPGCPGPSGMDGTRGPKGYKGESASQFGLPGPKGKPGGPGYPGRLGASGEQGLPGAQGPGGPPGRPGPPGLFGPTGCPGSPGGPGLQGPPGEAGDPGPRGVLGDPGAPGLPGIKGPSGSPGLNGLHGLKGQKGSKGASGSHEMGPPGPVGMPGPKGETGDPGNPGISPPGLFGERGAPGPPGPPGRAGLPGPPGAPGKALKGDIPDTGLPGDQGPPGPDGPRGVPGPPGPPGSVDLLKGEPGDCGLPGPPGLPGPPGPPGHKGFPGCDGKHGQKGPMGFPGPQGPPGSPGPPGDKGLPGPPGRRGPLGPPGSRGGDSNFR

Target ORF information:

RefSeq Version	XM_006047136.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis collagen type IV alpha 4 chain (COL4A4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047136.2

ORF Insert Sequence:

ATGGCACTGG TAAGGTGTCC TTTCAGGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTCTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT 
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT 
CAACCGGGAC CACCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCATT 
GGGATCCCAG GAGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC 
AAAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTAGATGG GATACCTGGG 
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA 
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGCCC CCCAGGCCTT 
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG 
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC 
CCAGCAGGCC CAGCGGGGTA CCCGGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT 
GGCAGTCCAG GTTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG 
GGTGAGGTTG GCCAACAAGG TTCTCCGGGA CCCACTCTGT TGGTGCAACC ACCTGATTCT 
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACCTCCTGGA 
CCACTGGGAC CCAGGGGAGA GCCTGGTATT GGAAGAAAAG GAGAGAAAGG GATTCCTGGG 
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG 
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT 
CCTGGAGACC GTGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC 
AAAGGCCCTC CAGGGGATCC AGGGCACCCC GGCCGCTATG GAGAAACAGG GGCTGTTGGA 
CCACGTGGTC CTCCTGGTGC CCCAGGTCGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA 
CCTCCTGGGC CACGAGGGTT TCCTGGTCAT CCAGGATTTC CAGGGGCAGC TGGGGTTCCT 
GGAAGAGCTG ATTCTGCTCC AGGAAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA 
GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGTTGTAT ATTGTACTGT TGGGCAACCC 
GGACCACAAG GAATAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA 
AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA 
GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA 
AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT 
GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA 
GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA 
CATGGCCGAG ATGGATATGC TGGAGAAAAG GGGGACACGG GGCCGCCTGG TGATCACGAG 
GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCAGGA 
CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA 
GCTCCAGGCT GCCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA 
ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA 
GGTCCAAAGG GCCTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAA 
AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTCCGTGGT 
GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGA 
GCTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACACC CAGGAGATCC TGCTATCGGC 
TACCCAGGAC TTCCGGGAAA GAGCGGACTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA 
GGCGATGCCG GGCGTCCAGG GACCACAGGG CCAGCTGGCA TGCCCGGATT TCCAGGTCTC 
CAAGGTCCCA AGGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCTACCTGGT 
CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC CGGGGCTCCA 
GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT 
GCTGGAATGA AGGGCCTTCC TGGAGTCCCG GGATGGCCGG GGGCAGATGG AACCCCAGGT 
CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG 
GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG 
GATGGACACC CTGGCAGAAA GGGAGAACAC GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA 
GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAATG 
GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAG CTTCTGGGGA TGGTGGATTC 
CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA 
AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCTGGAA GAAATGGACA GCCAGGGCTT 
CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GCAACATTGG TTTTCCAGGA 
TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT 
GGTACCAGAG GACCTAAAGG ATACAAAGGG GAATCTGCAA GTCAGTTTGG CCTACCTGGT 
CCAAAGGGTA AGCCAGGTGG CCCCGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG 
GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG 
TTTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA 
GAAGCGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGGGCACC AGGTCTTCCA 
GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG 
AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG 
CCTGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCAGGACTT 
TTTGGAGAAA GAGGTGCCCC GGGTCCCCCA GGACCCCCAG GGCGAGCTGG ACTGCCTGGT 
CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACACGGG TCTGCCTGGA 
GATCAGGGGC CTCCTGGCCC CGATGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT 
GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCGGG GCCTCCAGGT 
CTCCCAGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT 
GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA 
CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT 
TCCAGAGGTG GTGATTCCAA TTTCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	COL4A4
Entrez Gene ID	102389047
Full Name	collagen type IV alpha 4 chain
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

COL4A4 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

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