LOC103828778 cDNA ORF clone, Brassica rapa(field mustard)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103828778
Entrez Gene ID	103828778
Full Name	bystin-like
Gene Type	protein-coding
Organism	Brassica rapa(field mustard)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009104413.2	XP_009102661.2	bystin-like

Pathways from BioSystems
Gene Ontology

The following LOC103828778 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103828778 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBr158422	XM_009104413.2 Latest version!	Brassica rapa bystin-like (LOC103828778), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103828778 ( XM_009104413.2 ) cDNA ORF clone, Brassica rapa(field mustard) -> XP_009102661.2 Brassica rapa bystin-like (LOC103828778), mRNA.

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CloneID	OBr158422
Clone ID Related Accession (Same CDS sequence)	XM_009104413.2
Accession Version	XM_009104413.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-12
Organism	Brassica rapa(field mustard)
Product	bystin-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024801.1) and transcript sequence (GBDX01014307.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 13, 2016 this sequence version replaced XM_009104413.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brassica rapa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 737 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAATAAAT ACAGGCAAGT CTTGTACAAA GGCCATTTGT CTGAAATGTA TGTACCTTAC 
ATGGACCCAA GTGAGGATTG GTACTTCATC ACTTACCTTG ACTGTGGGGA ATTCGGCTGC 
GGCCAGTCGG CAGTGTCCCT TGAGCCGTAC ACCGATTGTC CTGCAGATGC CGTTTTCTTT 
GATGGTACTT TTGCGGGTCA AGACGGAACT CCCACAAACA TATCAAAAGC TATGTGCATT 
TTTGAAAAAA TTGCTGGAGA CATTATGTGG CGACATACCG AAGCAGAAGT TCCCGGCTTA 
GAAGTAAGAC CGGATGTAAG TCTTGTCGCC AGGATGGTGA CGACCGTGGG GAACTATGAC 
TACATAGTTG ATTACGAGTT CAAACCTAGC GGTGCCATCA AAATGGAGGT TGGTTTAACC 
GGAGTTTTAG AAGTCAAACC GGTGGAATAT GTTCACACAT CGGAGATCAA AGAAGATGAT 
ATCCACGGGA CAATTGTAGC GGACAACACC GTTGGAGTCA ACCACGACCA TTTTGTGACA 
TACCGTCTTG ATCTTGACAT CGATGGTACG GAAAATTCCT TCGTCCGCAC GGAACTTGTG 
ACCAAGAGAA CACCAAAATC TGTTAAGACA CCGAGAAAAA GCTATTGGAC AACGAATCGG 
AAGACTGTAA AAACCGAGGC AGAGGCTCGT GTGAAGCTAG GGCTGAGAGC GGAGGAGCTG 
ATGGTGGTTA ACCCTATCAA AACCCCAACT GCTTCTCTAT CTCGTGCCGT CTTCTGCAAA 
AAAAGAAAAG AAAAGAAGGG GTCCTTCTTC TCACAAGAAA CAATGGCGAA GAAACGTGAT 
AGAATAATCA ACACACAGCC CTTTATCACC GATGACGCCT CCGTCGCTTC TTCCCGGAAG 
CGTTCAAAGG TTCCCAAAAC CCATCAGCAA CAGGAGAAGT TGATTGAAGC TGGTATTAGC 
TCCAAGATCA TGAAGGTCGC TCTTGCACAG CAAAAGGAGA TTGCTGATGA AGAAAACGCC 
GAGAGGAACC CCAGTGGAGC CGTGTTTGCT GCTGCAGCCG CCAAGACTGC GGAAGAGGAG 
CAGAGAATCC TGGAGGAGGA GGATGAGAAA GATGATGATA TCGACGCTTT TGATGGAAAC 
TTCGAAGAAG ATGATAGCTA CCACCAAGAG GAGATCAATG AGGACGACGA GAAGCTGTTT 
GAGTCATTCT TTGTTAAGGA CGCCCCTCGT CAACGTACTC TTGCTGATAT CATCATCAAG 
AAAATCAAAG ACAACGATGC TGACTTAGCT GAAGAAGAGC GTCCTGACCC TGAGTTGGAC 
CCTAAAGTAA CTAAATTATA CAAAGGTGTT GCCAAGATCA TGAGTGAATA CACGATTGGG 
AAAATGCCGA AAGCGTTTGT GCTGATTACT AAGATGGAGC GCTGGGAGGA TGTGTTATAC 
TTGACCGAGC CGGAGAAATG GTCGCCTAAC GCTATGTATC AAGCAACCAG AATCTTTGCT 
CACCACTTAA AAAACAGCCA GATTCAGCGG TTTTACAGCT ACGTGTTGCT TCCTCGAGTC 
AGAGAAGATA TCAGAAAGAA CAAGAGGCTG CATTTTGCTC TCTACCAGGC TTTGAAGAAG 
TCTCTCTACA AGCCTAGTGC TTTCAATAAA GGCATACTCT TCCCGCTTTG TAAGTCTGGT 
ACATGCAGTC TCAGAGAAGC TGTGATTATA GGGAGCATTC TCGAAAAGTG TTCTATCCCT 
ATGCTTCACT CATGTGTTGC TTTGCTGAAC TTGGCTGAGA TGGAGTATTG TGGAACAACC 
AGCTACTTTA TAAAAACTCT TTTGGAGAAG AAGTATTGTA TGCCGTATCG AGTACTAGAT 
GCATTGGTTG CTCACTTCAT GAGATTTGTG GATGAGATAA GAGTTATGCC TGTGATTTGG 
CACCAGTCTC TTCTTACTTT TGTGCAACGG TACAAGTATG AGCTACTAAA GGAAGACAAA 
GAGCATTTCC AAACTCTCCT CAAGAGGCAA AGGCACCATC TTGTTACTCC TGAGATCCTG 
AGAGAGCTTC AGGGTAGCAG AAACCGTGGA GAGAAAGAAG ATGATCCTAT GTTGACTAAC 
TCTTCAGTTA CAACTATCAA CAACCCAATC AAAGAAGACA GGTTTGACAT TCCAGAGGTG 
CCAATGGAAG AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009102661.2
CDS	1..2178
Translation	MNKYRQVLYKGHLSEMYVPYMDPSEDWYFITYLDCGEFGCGQSAVSLEPYTDCPADAVFFDGTFAGQDGTPTNISKAMCIFEKIAGDIMWRHTEAEVPGLEVRPDVSLVARMVTTVGNYDYIVDYEFKPSGAIKMEVGLTGVLEVKPVEYVHTSEIKEDDIHGTIVADNTVGVNHDHFVTYRLDLDIDGTENSFVRTELVTKRTPKSVKTPRKSYWTTNRKTVKTEAEARVKLGLRAEELMVVNPIKTPTASLSRAVFCKKRKEKKGSFFSQETMAKKRDRIINTQPFITDDASVASSRKRSKVPKTHQQQEKLIEAGISSKIMKVALAQQKEIADEENAERNPSGAVFAAAAAKTAEEEQRILEEEDEKDDDIDAFDGNFEEDDSYHQEEINEDDEKLFESFFVKDAPRQRTLADIIIKKIKDNDADLAEEERPDPELDPKVTKLYKGVAKIMSEYTIGKMPKAFVLITKMERWEDVLYLTEPEKWSPNAMYQATRIFAHHLKNSQIQRFYSYVLLPRVREDIRKNKRLHFALYQALKKSLYKPSAFNKGILFPLCKSGTCSLREAVIIGSILEKCSIPMLHSCVALLNLAEMEYCGTTSYFIKTLLEKKYCMPYRVLDALVAHFMRFVDEIRVMPVIWHQSLLTFVQRYKYELLKEDKEHFQTLLKRQRHHLVTPEILRELQGSRNRGEKEDDPMLTNSSVTTINNPIKEDRFDIPEVPMEED

Target ORF information:

RefSeq Version	XM_009104413.2
Organism	Brassica rapa(field mustard)
Definition	Brassica rapa bystin-like (LOC103828778), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009104413.2

ORF Insert Sequence:

ATGAATAAAT ACAGGCAAGT CTTGTACAAA GGCCATTTGT CTGAAATGTA TGTACCTTAC 
ATGGACCCAA GTGAGGATTG GTACTTCATC ACTTACCTTG ACTGTGGGGA ATTCGGCTGC 
GGCCAGTCGG CAGTGTCCCT TGAGCCGTAC ACCGATTGTC CTGCAGATGC CGTTTTCTTT 
GATGGTACTT TTGCGGGTCA AGACGGAACT CCCACAAACA TATCAAAAGC TATGTGCATT 
TTTGAAAAAA TTGCTGGAGA CATTATGTGG CGACATACCG AAGCAGAAGT TCCCGGCTTA 
GAAGTAAGAC CGGATGTAAG TCTTGTCGCC AGGATGGTGA CGACCGTGGG GAACTATGAC 
TACATAGTTG ATTACGAGTT CAAACCTAGC GGTGCCATCA AAATGGAGGT TGGTTTAACC 
GGAGTTTTAG AAGTCAAACC GGTGGAATAT GTTCACACAT CGGAGATCAA AGAAGATGAT 
ATCCACGGGA CAATTGTAGC GGACAACACC GTTGGAGTCA ACCACGACCA TTTTGTGACA 
TACCGTCTTG ATCTTGACAT CGATGGTACG GAAAATTCCT TCGTCCGCAC GGAACTTGTG 
ACCAAGAGAA CACCAAAATC TGTTAAGACA CCGAGAAAAA GCTATTGGAC AACGAATCGG 
AAGACTGTAA AAACCGAGGC AGAGGCTCGT GTGAAGCTAG GGCTGAGAGC GGAGGAGCTG 
ATGGTGGTTA ACCCTATCAA AACCCCAACT GCTTCTCTAT CTCGTGCCGT CTTCTGCAAA 
AAAAGAAAAG AAAAGAAGGG GTCCTTCTTC TCACAAGAAA CAATGGCGAA GAAACGTGAT 
AGAATAATCA ACACACAGCC CTTTATCACC GATGACGCCT CCGTCGCTTC TTCCCGGAAG 
CGTTCAAAGG TTCCCAAAAC CCATCAGCAA CAGGAGAAGT TGATTGAAGC TGGTATTAGC 
TCCAAGATCA TGAAGGTCGC TCTTGCACAG CAAAAGGAGA TTGCTGATGA AGAAAACGCC 
GAGAGGAACC CCAGTGGAGC CGTGTTTGCT GCTGCAGCCG CCAAGACTGC GGAAGAGGAG 
CAGAGAATCC TGGAGGAGGA GGATGAGAAA GATGATGATA TCGACGCTTT TGATGGAAAC 
TTCGAAGAAG ATGATAGCTA CCACCAAGAG GAGATCAATG AGGACGACGA GAAGCTGTTT 
GAGTCATTCT TTGTTAAGGA CGCCCCTCGT CAACGTACTC TTGCTGATAT CATCATCAAG 
AAAATCAAAG ACAACGATGC TGACTTAGCT GAAGAAGAGC GTCCTGACCC TGAGTTGGAC 
CCTAAAGTAA CTAAATTATA CAAAGGTGTT GCCAAGATCA TGAGTGAATA CACGATTGGG 
AAAATGCCGA AAGCGTTTGT GCTGATTACT AAGATGGAGC GCTGGGAGGA TGTGTTATAC 
TTGACCGAGC CGGAGAAATG GTCGCCTAAC GCTATGTATC AAGCAACCAG AATCTTTGCT 
CACCACTTAA AAAACAGCCA GATTCAGCGG TTTTACAGCT ACGTGTTGCT TCCTCGAGTC 
AGAGAAGATA TCAGAAAGAA CAAGAGGCTG CATTTTGCTC TCTACCAGGC TTTGAAGAAG 
TCTCTCTACA AGCCTAGTGC TTTCAATAAA GGCATACTCT TCCCGCTTTG TAAGTCTGGT 
ACATGCAGTC TCAGAGAAGC TGTGATTATA GGGAGCATTC TCGAAAAGTG TTCTATCCCT 
ATGCTTCACT CATGTGTTGC TTTGCTGAAC TTGGCTGAGA TGGAGTATTG TGGAACAACC 
AGCTACTTTA TAAAAACTCT TTTGGAGAAG AAGTATTGTA TGCCGTATCG AGTACTAGAT 
GCATTGGTTG CTCACTTCAT GAGATTTGTG GATGAGATAA GAGTTATGCC TGTGATTTGG 
CACCAGTCTC TTCTTACTTT TGTGCAACGG TACAAGTATG AGCTACTAAA GGAAGACAAA 
GAGCATTTCC AAACTCTCCT CAAGAGGCAA AGGCACCATC TTGTTACTCC TGAGATCCTG 
AGAGAGCTTC AGGGTAGCAG AAACCGTGGA GAGAAAGAAG ATGATCCTAT GTTGACTAAC 
TCTTCAGTTA CAACTATCAA CAACCCAATC AAAGAAGACA GGTTTGACAT TCCAGAGGTG 
CCAATGGAAG AGGACTGA