PIKFYVE cDNA ORF clone, Bos taurus(cattle)

The following PIKFYVE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIKFYVE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa02962 XM_024978669.1
Latest version!
Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa02962 XM_010802278.2
Latest version!
Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa227599 XM_024978667.1
Latest version!
Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa227600 XM_024978671.1
Latest version!
Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa87982 XM_024978666.1
Latest version!
Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa87982 XM_015458891.1
Latest version!
Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa227598 XM_024978658.1
Latest version!
Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227596 XM_024978638.1
Latest version!
Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa227597 XM_024978640.1
Latest version!
Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa16660 NM_001205467.1
Latest version!
Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa16660 XM_005202762.4
Latest version!
Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa16660 XM_024978630.1
Latest version!
Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa58662 XM_024978618.1
Latest version!
Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa58662 XM_010802273.3
Latest version!
Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa58662 XM_010802271.3
Latest version!
Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa58662 XM_010802272.3
Latest version!
Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa58663 XM_010802274.2
Latest version!
Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa58663 XM_024978627.1
Latest version!
Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa58664 XM_010802275.2
Latest version!
Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa58664 XM_024978643.1
Latest version!
Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa58665 XM_010802276.2
Latest version!
Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa58665 XM_024978647.1
Latest version!
Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa58666 XM_024978654.1
Latest version!
Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa58666 XM_010802277.2
Latest version!
Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
OBa58662 XM_010802271.2 Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa16660 XM_005202762.3 Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa58662 XM_010802273.2 Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa58662 XM_010802272.2 Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa02962
    Clone ID Related Accession (Same CDS sequence) XM_010802278.2 , XM_024978669.1
    Accession Version XM_010802278.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802278.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGATAAGAAA
    GAACACTCAC TGCTAAGAAG AAGTGAAATC CAAGATAATG AGGAGATGGA GAGTGCCTGG
    TTGTTGTTGT TATTTTTCCC CCCTTCTAGT TTTATTGAGA TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800580.1
    CDS212..1996
    Translation

    Target ORF information:

    RefSeq Version XM_010802278.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802278.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGATAAGAAA
    GAACACTCAC TGCTAAGAAG AAGTGAAATC CAAGATAATG AGGAGATGGA GAGTGCCTGG
    TTGTTGTTGT TATTTTTCCC CCCTTCTAGT TTTATTGAGA TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa02962
    Clone ID Related Accession (Same CDS sequence) XM_010802278.2 , XM_024978669.1
    Accession Version XM_024978669.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGATAAGAAA
    GAACACTCAC TGCTAAGAAG AAGTGAAATC CAAGATAATG AGGAGATGGA GAGTGCCTGG
    TTGTTGTTGT TATTTTTCCC CCCTTCTAGT TTTATTGAGA TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834437.1
    CDS155..1939
    Translation

    Target ORF information:

    RefSeq Version XM_024978669.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978669.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGATAAGAAA
    GAACACTCAC TGCTAAGAAG AAGTGAAATC CAAGATAATG AGGAGATGGA GAGTGCCTGG
    TTGTTGTTGT TATTTTTCCC CCCTTCTAGT TTTATTGAGA TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227599
    Clone ID Related Accession (Same CDS sequence) XM_024978667.1
    Accession Version XM_024978667.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1806bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGGTAATTTT
    ATCAAAAGCT ATACATGGGA TGACGTTTCT TGTTCATGGA GATATACTGT GGGCCCAGGA
    TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834435.1
    CDS155..1960
    Translation

    Target ORF information:

    RefSeq Version XM_024978667.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978667.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGGTAATTTT
    ATCAAAAGCT ATACATGGGA TGACGTTTCT TGTTCATGGA GATATACTGT GGGCCCAGGA
    TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227600
    Clone ID Related Accession (Same CDS sequence) XM_024978671.1
    Accession Version XM_024978671.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAAAGAACA
    CTCACTGCTA AGAAGAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834439.1
    CDS155..1855
    Translation

    Target ORF information:

    RefSeq Version XM_024978671.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978671.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAAAGAACA
    CTCACTGCTA AGAAGAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87982
    Clone ID Related Accession (Same CDS sequence) XM_024978666.1 , XM_015458891.1
    Accession Version XM_024978666.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCATTT TGGAACGAGT TAGCCGGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834434.1
    CDS155..2545
    Translation

    Target ORF information:

    RefSeq Version XM_024978666.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978666.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCATTT TGGAACGAGT TAGCCGGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87982
    Clone ID Related Accession (Same CDS sequence) XM_024978666.1 , XM_015458891.1
    Accession Version XM_015458891.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCATTT TGGAACGAGT TAGCCGGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314377.1
    CDS134..2524
    Translation

    Target ORF information:

    RefSeq Version XM_015458891.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458891.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCATTT TGGAACGAGT TAGCCGGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227598
    Clone ID Related Accession (Same CDS sequence) XM_024978658.1
    Accession Version XM_024978658.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGTACCAT CATACGAGAC GTCTGTCAGT CCCCAGGCTA ACCGAACGTA TGTTCGGACA 
    GAGACCACTG AGGATGAACG CAAAATTCTT CTGGACAGTG TTCAGTTAAA AGACCTATGG
    AAGAAAATCT GCCATCACAG CAGTGGAATG GAGTTTCAGG ATCACCGCTA CTGGCTGAGA
    ACACACCCCA ACTGCATTGT AGGAAAGGAG TTAGTCAACT GGTTAATCCG AAATGGACAC
    ATTGCTACAA GGGCACAGGC TATAGCAATT GGACAGGCAA TGGTGGATGG ACGTTGGCTG
    GATTGTGTCA GTCACCACGA CCAGCTCTTC AGGGATGAGT ATGCGCTGTA CAGGCCGCTG
    CAGAGTACAG AATTTTCCGA GACCCCTTCT CCGGACAGTG ACTCGGTGAA CTCTGTGGAA
    GGACACTCTG AGCCATCCTG GTTTAAAGAC ATCAAGTTTG ATGACAGTGA CACTGAGCAG
    ATAGCTGAAG AAGGTGACGA TAATTTGACT AACTCTGCCA GTCCTAGCAA GCGCACATCA
    GTCAGCAGTT TCCAGTCCAC AGTGGACAGT GACTCAGCCG CTTCCATCAG CCTGAACGTG
    GAGCTGGACA ACGTGAACTT CCATATCAAG AAGCCCTCCA AGTACCCACA TGTGCCCCCT
    CACCCTGCTG ACCAAAAAGA GTATTTGATT TCTGACAATG GAGGACAACA GCTATCAATA
    AGTGACGCCT TCATCAAAGA ATCCTTATTT AACCGTCGAG TTGAGGAAAA ATCCAAAGAA
    CTGCCTTTCA CACCTTTGGG CTGGCATCAT AACAACCTGG AGCTGCTGAG GGAGGAGAAT
    GGAGAGAAAC AAGCTATGGA GAGGTTGCTT TCAGCTAATC ATAACCACAT GATGGCGCTG
    CTCCAACAGT TGCTCCATAG TGAGTCATTG TCAACGTCTT GGAGGGACAT CATCGTATCC
    CTGGTCTGCC AGGTTGTTCA GACAGTCCGA CCTGACGTCA AGAACCGGGA TGATGACATG
    GATATCCGCC AGTTTGTCCA CATCAAAAAA ATCCCAGGTG GAAAGAAATT TGATTCTGTG
    GTTGTCAATG GATTTGTTTG CACCAAGAAT ATTGCACATA AAAAGATGAA TTCTTCTATT
    AAAAACCCCA AAATTCTTCT GCTGAAATGT TCCATTGAAT ATCTCTACAG AGAAGAAACC
    AAATTTACTT GCATTGATCC TATTGTGCTT CAGGAAAGGG AATTCCTAAA GAATTATGTT
    CAGCGAATAG TTGATGTTCG ACCCACACTG GTTCTGGTTG AGAAAACGGT ATCTCGAATT
    GCACAGGATA TGTTGCTGGA ACACGGCATT ACTTTGGTCA TTAATGTAAA ATCACAAGTT
    TTGGAACGAG TTAGCCGGAT GACCCAAGGT GATTTGGTGA TGTCAATGGA CCAGCTGCTT
    ACCAAACCCC ACCTGGGCAC CTGTCACAAA TTTTACATGC AGATGTTTCA GTTGCCTAAT
    GAACAAACCA AGACACTGAT GTTTTTTGAA GGCTGTCCAC AGCACCTGGG CTGTACAATC
    AAGCTTAGAG GAGGCTCTGA TTATGAGCTG GCTCGAGTTA AGGAGATCTT AATATTTATG
    ATCTGTGTAG CTTATCATTC TCAACTAGAA ATCTCCTTCC TTATGGATGA ATTTGCTATG
    CCTCCTACAT TAACGCAAAA CCCTTCCTTC CATTCTCTGA TTGAAGGACA GGAGGATGAA
    GGGGCTTCAC AGGAGCCATT CAGCGGAAGT CCCCTCCCCC GGGAGCCTGA CTTCCCCCCA
    GACTTTCTGC CTTCTGATGA TGGCAGTTTG CTAGAAACAA GGATTCTGTT TGAGAAGGGT
    GACCAGGAGA ATAAGAGTGT GCCACAGGAT GTTGCCTCTT TGAAGCCTCA AGAGCATGCC
    CCAGCAGCTT GCCCGATGGG TGCCCCCTGT GCTCTCTTTC CATCAGTACC AGAGTCATTG
    CTTCCGCTCC ATGTGGATGA CCAACAGGAC GCCATAGGCA GTGAGCAGCC AGAGGCTTTG
    CAGCAAACAG AGGAGCTTCC GGATCCCACA AGCCAGATGA GAGCCTTTAG AGACCCTCTG
    CAGGATGACA CTGGCCTGTA TGTTACCGAG GAAGTTACCT CTTCTGAAGA TAAACGGAAG
    ACTGATTCTT TGACCTTTAA GCAAGAGTTG AAAGATGTGA TCCTCTGTAT CTCGCCAGTC
    ATCACATTCC GGGAGCCCTT CCTTTTAACT GACAAGGGCA TGAGATGCTC TACCCGAGAT
    TATTTTGCGG AGCAGGTTTA CTGGTCTCCT CTCCTCAATA AGGAGTTCAA GGAAATGGAG
    AGCAGGCGAA AGAAACAGAT GCTTAGGGAT CTCTCTGGCC TTCAGGGCAT GAATGGAAGT
    GTTCAGGCCA AGTCCATTCA AGTCCTGCCC TCACATGAGC TCGTGAGCAC CAGAATTGCT
    GAGCACCTGG GGGACAGCCA GAGCTTGGGG AGAATGCTAG CCGATTATCG GGCCAGAGGA
    GGAAGAATCC AGCAAAAAAA CTCAGACCCT TTTGCTTACT CAAAGGAGGC ATCAAGCACC
    TCAAGTGGAA AATCAGGAAG CAGAACTGAA GGTGATGAAG AAAAAGGGTT GATTACCAGT
    GATGCAGTGT GGTCGACAAA GGTGGACTGT CTGAACCCTG TCAACCACCA GAGGCTGTGT
    GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC AGCAACGCTC CCAGTGCCTG TGTCAGTCCT
    TGGATTGTAA CAATGGAATT TTATGGAAAA AATGATCTTA CATTAGGAAT ATTTTTAGAG
    AGATACTGTT TCCGGCCTTC CTATCAGTGC CCTAGCATGT TCTGTGATAC CCCCATGGTG
    CACCACATTC GACGCTTTGT CCATGGCCAA GGCTGTGTGC AGATAATCCT AAAGGAGCTG
    GATTCTCCAG TACCTGGATA TCAGCATACA ATTCTCACCT ACTCCTGGTG CAGAATGTGC
    AAACAGGTAA CACCAGTTGT TGCTCTTTCC AATGAGTCTT GGTCCATGTC GTTTGCAAAA
    TACCTGGAAC TTAGGTTTTA TGGGCACCAG TACACCCGCA GAGCCAATGC TGAGCCATGT
    GGTCACTCCA TCCACCACGA TTATCACCAG TATTTCTCCT ATAACCAGAT GGTGGCGTCT
    TTCAGTTATT CTCCCATTCG GCTTCTTGAA GTGTGTGTTC CACTTCCCAA AATATTCATT
    AAACGTCAAG TCCCACTAAA GGTGTCCCTT CTTCAGGATC TGAAGGACTT CTTTCAGAAA
    GTTTCACAGG TATATCTTGC TGTGGATGAA AGACTTGCGT CTTTGAAAAC TGATACATTT
    AGCAAATCAA GAGAGGAAAA AATGGAAGAT ATCTTTGCAC AAAAAGAGAT GGAAGAAAGT
    GAGTTCAAGA GCTGGACTGA GAAGATGCAG GCGCGGCTCC TGTCTTCCTC CATGGATACC
    CCCCAGCAAC TGCAGTCAGT CTTCGAGTCC CTCATCGCCA AGAAACAAAG CCTCTGTGAG
    GTGCTGCAAG CTTGGAATAA CAGGCTGCAA GACCTTTTCC AACAAGAAAA GGGTAGGAAG
    CGGCCTTCAG TTCCTCCAAG TCCTGGAAGA CTGAGACAAG GTGAAGAAAG CAAGATAAGT
    GCAGTGGATG CATCTCCACG GAATGCTTCT CCAGGACTTC CAAATGGAGA AAAAGAGGAC
    CGCTTCTTAG CCACCCTTTC CAGTCAGAGC TCCACCAGTT CCCCCCATCT CCAGCTGCCT
    ACTTCTCCTG AAGGCGTGCC TGAGCAGGTC ATGGGAGGGC CCCCAGATCT CGATACTGCC
    AGCAGTTCTG AAGATGTATT TGATGGACAT TTGCTGGGAT CTACAGACAG CCAGGTGAAG
    GAAAAATCAA CCATGAAAGC CATCTTTGCA AATTTGCTTC CAGGAAATAG CTATAACCCT
    ATTCCATTTC CTTTCGATCC AGATAAACAC TACTTAATGT ATGAACATGA ACGAGTGCCC
    ATTGCAGTCT GTGAGAAGGA GCCCAGCTCC ATCATTGCTT TTGCTCTCAG TTGTAAAGAA
    TACCGGAATG CCTTAGAGGA ATTGTCCAAA GCCACTCAGC GGAACAGTGC TGAAGAAGGG
    CTTCCAACAA ACAGTACTTT AGACAGCAGA CCAAAGAGTA GCAGCCCAAT TAGACTACCT
    GAGATCAGTG GAGGACAGAC AAACCGTGCG GTAGAAGCAG AACCACAGCC AACCAAAAAG
    GCTTCTGGAA TGTTGTCCTT CTTCCGAGGG ACAGCAGGGA AAAGCCCTGA CCTCTCTTCC
    CAGAAGAGAG AGACCTTACG TGGAGCAGAT AGTGCTTACT ACCAAGTGGG GCAGACGGGC
    AAGGAGGGGA CGGAGAATCA AGGCATTGAG CCTCAAGATG AAGTAGATGG AGGAGATACA
    CAGAAGAAAC AACTTACAAA TCCTCATGTG GAGCTTCAAT TTTCGGATGC CAACGCCAAG
    TTTTACTGTC GGCTCTACTA TGCGGGAGAG TTTCATAAGA TGCGAGAAGT GATTCTGGGC
    AGCAGCGAAG AAGATTTCAT TCGTTCCCTT TCTCACTCTT CTCCGTGGCA GGCCCGAGGA
    GGCAAATCAG GAGCTGCCTT TTACGCTACT GAAGATGACA GATTCATTTT GAAGCAAATG
    CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC TTCGCACCAC ACTACTTCAA TTACATTACA
    AATGCCGTTC AGCAAAAGAG GCCTACTGCA TTGGCCAAGA TTCTTGGAGT TTACAGAATT
    GGTTATAAGA ATTCTCAGAA CAACACTGAG AAGAAGCTAG ATCTCCTTGT CATGGAAAAT
    CTTTTCTACG GGAGAAAGAT GGCACAGGTT TTTGACTTGA AGGGTTCACT TAGGAATCGA
    AATGTAAAAA CCGATACCGG GAAAGAGAGC TGTGATGTTG TTCTTCTGGA TGAAAATCTC
    TTAAAGATGG TTCGAGACAA CCCTCTCTAT ATCCGTTCTC ATTCCAAAGC TGTGCTGAGA
    GCCTCGATCC GGAGCGACTC CCACTTCCTT TCTAGCCACC TCATTATAGA CTATTCTTTG
    CTAGTTGGGC GAGACGATAC CAGCAATGAG CTAGTAGTTG GAATTATAGA TTATATCCGA
    ACATTTACCT GGGACAAAAA GCTTGAGATG GTTGTGAAAT CAACGGGAAT TTTAGGAGGA
    CAAGGTAAAA TGCCGACTGT GGTCTCTCCA GAGTTGTACA GGACTAGATT CTGTGAAGCC
    ATGGACAAGT ATTTCCTGAT GGTGCCAGAC CACTGGACAG GCTTGGGTCT GAACTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834426.1
    CDS1207..6546
    Translation

    Target ORF information:

    RefSeq Version XM_024978658.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978658.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGTACCAT CATACGAGAC GTCTGTCAGT CCCCAGGCTA ACCGAACGTA TGTTCGGACA 
    GAGACCACTG AGGATGAACG CAAAATTCTT CTGGACAGTG TTCAGTTAAA AGACCTATGG
    AAGAAAATCT GCCATCACAG CAGTGGAATG GAGTTTCAGG ATCACCGCTA CTGGCTGAGA
    ACACACCCCA ACTGCATTGT AGGAAAGGAG TTAGTCAACT GGTTAATCCG AAATGGACAC
    ATTGCTACAA GGGCACAGGC TATAGCAATT GGACAGGCAA TGGTGGATGG ACGTTGGCTG
    GATTGTGTCA GTCACCACGA CCAGCTCTTC AGGGATGAGT ATGCGCTGTA CAGGCCGCTG
    CAGAGTACAG AATTTTCCGA GACCCCTTCT CCGGACAGTG ACTCGGTGAA CTCTGTGGAA
    GGACACTCTG AGCCATCCTG GTTTAAAGAC ATCAAGTTTG ATGACAGTGA CACTGAGCAG
    ATAGCTGAAG AAGGTGACGA TAATTTGACT AACTCTGCCA GTCCTAGCAA GCGCACATCA
    GTCAGCAGTT TCCAGTCCAC AGTGGACAGT GACTCAGCCG CTTCCATCAG CCTGAACGTG
    GAGCTGGACA ACGTGAACTT CCATATCAAG AAGCCCTCCA AGTACCCACA TGTGCCCCCT
    CACCCTGCTG ACCAAAAAGA GTATTTGATT TCTGACAATG GAGGACAACA GCTATCAATA
    AGTGACGCCT TCATCAAAGA ATCCTTATTT AACCGTCGAG TTGAGGAAAA ATCCAAAGAA
    CTGCCTTTCA CACCTTTGGG CTGGCATCAT AACAACCTGG AGCTGCTGAG GGAGGAGAAT
    GGAGAGAAAC AAGCTATGGA GAGGTTGCTT TCAGCTAATC ATAACCACAT GATGGCGCTG
    CTCCAACAGT TGCTCCATAG TGAGTCATTG TCAACGTCTT GGAGGGACAT CATCGTATCC
    CTGGTCTGCC AGGTTGTTCA GACAGTCCGA CCTGACGTCA AGAACCGGGA TGATGACATG
    GATATCCGCC AGTTTGTCCA CATCAAAAAA ATCCCAGGTG GAAAGAAATT TGATTCTGTG
    GTTGTCAATG GATTTGTTTG CACCAAGAAT ATTGCACATA AAAAGATGAA TTCTTCTATT
    AAAAACCCCA AAATTCTTCT GCTGAAATGT TCCATTGAAT ATCTCTACAG AGAAGAAACC
    AAATTTACTT GCATTGATCC TATTGTGCTT CAGGAAAGGG AATTCCTAAA GAATTATGTT
    CAGCGAATAG TTGATGTTCG ACCCACACTG GTTCTGGTTG AGAAAACGGT ATCTCGAATT
    GCACAGGATA TGTTGCTGGA ACACGGCATT ACTTTGGTCA TTAATGTAAA ATCACAAGTT
    TTGGAACGAG TTAGCCGGAT GACCCAAGGT GATTTGGTGA TGTCAATGGA CCAGCTGCTT
    ACCAAACCCC ACCTGGGCAC CTGTCACAAA TTTTACATGC AGATGTTTCA GTTGCCTAAT
    GAACAAACCA AGACACTGAT GTTTTTTGAA GGCTGTCCAC AGCACCTGGG CTGTACAATC
    AAGCTTAGAG GAGGCTCTGA TTATGAGCTG GCTCGAGTTA AGGAGATCTT AATATTTATG
    ATCTGTGTAG CTTATCATTC TCAACTAGAA ATCTCCTTCC TTATGGATGA ATTTGCTATG
    CCTCCTACAT TAACGCAAAA CCCTTCCTTC CATTCTCTGA TTGAAGGACA GGAGGATGAA
    GGGGCTTCAC AGGAGCCATT CAGCGGAAGT CCCCTCCCCC GGGAGCCTGA CTTCCCCCCA
    GACTTTCTGC CTTCTGATGA TGGCAGTTTG CTAGAAACAA GGATTCTGTT TGAGAAGGGT
    GACCAGGAGA ATAAGAGTGT GCCACAGGAT GTTGCCTCTT TGAAGCCTCA AGAGCATGCC
    CCAGCAGCTT GCCCGATGGG TGCCCCCTGT GCTCTCTTTC CATCAGTACC AGAGTCATTG
    CTTCCGCTCC ATGTGGATGA CCAACAGGAC GCCATAGGCA GTGAGCAGCC AGAGGCTTTG
    CAGCAAACAG AGGAGCTTCC GGATCCCACA AGCCAGATGA GAGCCTTTAG AGACCCTCTG
    CAGGATGACA CTGGCCTGTA TGTTACCGAG GAAGTTACCT CTTCTGAAGA TAAACGGAAG
    ACTGATTCTT TGACCTTTAA GCAAGAGTTG AAAGATGTGA TCCTCTGTAT CTCGCCAGTC
    ATCACATTCC GGGAGCCCTT CCTTTTAACT GACAAGGGCA TGAGATGCTC TACCCGAGAT
    TATTTTGCGG AGCAGGTTTA CTGGTCTCCT CTCCTCAATA AGGAGTTCAA GGAAATGGAG
    AGCAGGCGAA AGAAACAGAT GCTTAGGGAT CTCTCTGGCC TTCAGGGCAT GAATGGAAGT
    GTTCAGGCCA AGTCCATTCA AGTCCTGCCC TCACATGAGC TCGTGAGCAC CAGAATTGCT
    GAGCACCTGG GGGACAGCCA GAGCTTGGGG AGAATGCTAG CCGATTATCG GGCCAGAGGA
    GGAAGAATCC AGCAAAAAAA CTCAGACCCT TTTGCTTACT CAAAGGAGGC ATCAAGCACC
    TCAAGTGGAA AATCAGGAAG CAGAACTGAA GGTGATGAAG AAAAAGGGTT GATTACCAGT
    GATGCAGTGT GGTCGACAAA GGTGGACTGT CTGAACCCTG TCAACCACCA GAGGCTGTGT
    GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC AGCAACGCTC CCAGTGCCTG TGTCAGTCCT
    TGGATTGTAA CAATGGAATT TTATGGAAAA AATGATCTTA CATTAGGAAT ATTTTTAGAG
    AGATACTGTT TCCGGCCTTC CTATCAGTGC CCTAGCATGT TCTGTGATAC CCCCATGGTG
    CACCACATTC GACGCTTTGT CCATGGCCAA GGCTGTGTGC AGATAATCCT AAAGGAGCTG
    GATTCTCCAG TACCTGGATA TCAGCATACA ATTCTCACCT ACTCCTGGTG CAGAATGTGC
    AAACAGGTAA CACCAGTTGT TGCTCTTTCC AATGAGTCTT GGTCCATGTC GTTTGCAAAA
    TACCTGGAAC TTAGGTTTTA TGGGCACCAG TACACCCGCA GAGCCAATGC TGAGCCATGT
    GGTCACTCCA TCCACCACGA TTATCACCAG TATTTCTCCT ATAACCAGAT GGTGGCGTCT
    TTCAGTTATT CTCCCATTCG GCTTCTTGAA GTGTGTGTTC CACTTCCCAA AATATTCATT
    AAACGTCAAG TCCCACTAAA GGTGTCCCTT CTTCAGGATC TGAAGGACTT CTTTCAGAAA
    GTTTCACAGG TATATCTTGC TGTGGATGAA AGACTTGCGT CTTTGAAAAC TGATACATTT
    AGCAAATCAA GAGAGGAAAA AATGGAAGAT ATCTTTGCAC AAAAAGAGAT GGAAGAAAGT
    GAGTTCAAGA GCTGGACTGA GAAGATGCAG GCGCGGCTCC TGTCTTCCTC CATGGATACC
    CCCCAGCAAC TGCAGTCAGT CTTCGAGTCC CTCATCGCCA AGAAACAAAG CCTCTGTGAG
    GTGCTGCAAG CTTGGAATAA CAGGCTGCAA GACCTTTTCC AACAAGAAAA GGGTAGGAAG
    CGGCCTTCAG TTCCTCCAAG TCCTGGAAGA CTGAGACAAG GTGAAGAAAG CAAGATAAGT
    GCAGTGGATG CATCTCCACG GAATGCTTCT CCAGGACTTC CAAATGGAGA AAAAGAGGAC
    CGCTTCTTAG CCACCCTTTC CAGTCAGAGC TCCACCAGTT CCCCCCATCT CCAGCTGCCT
    ACTTCTCCTG AAGGCGTGCC TGAGCAGGTC ATGGGAGGGC CCCCAGATCT CGATACTGCC
    AGCAGTTCTG AAGATGTATT TGATGGACAT TTGCTGGGAT CTACAGACAG CCAGGTGAAG
    GAAAAATCAA CCATGAAAGC CATCTTTGCA AATTTGCTTC CAGGAAATAG CTATAACCCT
    ATTCCATTTC CTTTCGATCC AGATAAACAC TACTTAATGT ATGAACATGA ACGAGTGCCC
    ATTGCAGTCT GTGAGAAGGA GCCCAGCTCC ATCATTGCTT TTGCTCTCAG TTGTAAAGAA
    TACCGGAATG CCTTAGAGGA ATTGTCCAAA GCCACTCAGC GGAACAGTGC TGAAGAAGGG
    CTTCCAACAA ACAGTACTTT AGACAGCAGA CCAAAGAGTA GCAGCCCAAT TAGACTACCT
    GAGATCAGTG GAGGACAGAC AAACCGTGCG GTAGAAGCAG AACCACAGCC AACCAAAAAG
    GCTTCTGGAA TGTTGTCCTT CTTCCGAGGG ACAGCAGGGA AAAGCCCTGA CCTCTCTTCC
    CAGAAGAGAG AGACCTTACG TGGAGCAGAT AGTGCTTACT ACCAAGTGGG GCAGACGGGC
    AAGGAGGGGA CGGAGAATCA AGGCATTGAG CCTCAAGATG AAGTAGATGG AGGAGATACA
    CAGAAGAAAC AACTTACAAA TCCTCATGTG GAGCTTCAAT TTTCGGATGC CAACGCCAAG
    TTTTACTGTC GGCTCTACTA TGCGGGAGAG TTTCATAAGA TGCGAGAAGT GATTCTGGGC
    AGCAGCGAAG AAGATTTCAT TCGTTCCCTT TCTCACTCTT CTCCGTGGCA GGCCCGAGGA
    GGCAAATCAG GAGCTGCCTT TTACGCTACT GAAGATGACA GATTCATTTT GAAGCAAATG
    CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC TTCGCACCAC ACTACTTCAA TTACATTACA
    AATGCCGTTC AGCAAAAGAG GCCTACTGCA TTGGCCAAGA TTCTTGGAGT TTACAGAATT
    GGTTATAAGA ATTCTCAGAA CAACACTGAG AAGAAGCTAG ATCTCCTTGT CATGGAAAAT
    CTTTTCTACG GGAGAAAGAT GGCACAGGTT TTTGACTTGA AGGGTTCACT TAGGAATCGA
    AATGTAAAAA CCGATACCGG GAAAGAGAGC TGTGATGTTG TTCTTCTGGA TGAAAATCTC
    TTAAAGATGG TTCGAGACAA CCCTCTCTAT ATCCGTTCTC ATTCCAAAGC TGTGCTGAGA
    GCCTCGATCC GGAGCGACTC CCACTTCCTT TCTAGCCACC TCATTATAGA CTATTCTTTG
    CTAGTTGGGC GAGACGATAC CAGCAATGAG CTAGTAGTTG GAATTATAGA TTATATCCGA
    ACATTTACCT GGGACAAAAA GCTTGAGATG GTTGTGAAAT CAACGGGAAT TTTAGGAGGA
    CAAGGTAAAA TGCCGACTGT GGTCTCTCCA GAGTTGTACA GGACTAGATT CTGTGAAGCC
    ATGGACAAGT ATTTCCTGAT GGTGCCAGAC CACTGGACAG GCTTGGGTCT GAACTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227596
    Clone ID Related Accession (Same CDS sequence) XM_024978638.1
    Accession Version XM_024978638.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6243bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGATC AGCCAGCATT ACTAATCTGT CACTGGATCG ATCTGCGTCT
    CCAATGGTAC CATCATACGA GACGTCTGTC AGTCCCCAGG CTAACCGAAC GTATGTTCGG
    ACAGAGACCA CTGAGGATGA ACGCAAAATT CTTCTGGACA GTGTTCAGTT AAAAGACCTA
    TGGAAGAAAA TCTGCCATCA CAGCAGTGGA ATGGAGTTTC AGGATCACCG CTACTGGCTG
    AGAACACACC CCAACTGCAT TGTAGGAAAG GAGTTAGTCA ACTGGTTAAT CCGAAATGGA
    CACATTGCTA CAAGGGCACA GGCTATAGCA ATTGGACAGG CAATGGTGGA TGGACGTTGG
    CTGGATTGTG TCAGTCACCA CGACCAGCTC TTCAGGGATG AGTATGCGCT GTACAGGCCG
    CTGCAGAGTA CAGAATTTTC CGAGACCCCT TCTCCGGACA GTGACTCGGT GAACTCTGTG
    GAAGGACACT CTGAGCCATC CTGGTTTAAA GACATCAAGT TTGATGACAG TGACACTGAG
    CAGATAGCTG AAGAAGGTGA CGATAATTTG ACTAACTCTG CCAGTCCTAG CAAGCGCACA
    TCAGTCAGCA GTTTCCAGTC CACAGTGGAC AGTGACTCAG CCGCTTCCAT CAGCCTGAAC
    GTGGAGCTGG ACAACGTGAA CTTCCATATC AAGAAGCCCT CCAAGTACCC ACATGTGCCC
    CCTCACCCTG CTGACCAAAA AGAGTATTTG ATTTCTGACA ATGGAGGACA ACAGCTATCA
    ATAAGTGACG CCTTCATCAA AGAATCCTTA TTTAACCGTC GAGTTGAGGA AAAATCCAAA
    GAACTGCCTT TCACACCTTT GGGCTGGCAT CATAACAACC TGGAGCTGCT GAGGGAGGAG
    AATGGAGAGA AACAAGCTAT GGAGAGGTTG CTTTCAGCTA ATCATAACCA CATGATGGCG
    CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA TTGTCAACGT CTTGGAGGGA CATCATCGTA
    TCCCTGGTCT GCCAGGTTGT TCAGACAGTC CGACCTGACG TCAAGAACCG GGATGATGAC
    ATGGATATCC GCCAGTTTGT CCACATCAAA AAAATCCCAG GTGGAAAGAA ATTTGATTCT
    GTGGTTGTCA ATGGATTTGT TTGCACCAAG AATATTGCAC ATAAAAAGAT GAATTCTTCT
    ATTAAAAACC CCAAAATTCT TCTGCTGAAA TGTTCCATTG AATATCTCTA CAGAGAAGAA
    ACCAAATTTA CTTGCATTGA TCCTATTGTG CTTCAGGAAA GGGAATTCCT AAAGAATTAT
    GTTCAGCGAA TAGTTGATGT TCGACCCACA CTGGTTCTGG TTGAGAAAAC GGTATCTCGA
    ATTGCACAGG ATATGTTGCT GGAACACGGC ATTACTTTGG TCATTAATGT AAAATCACAA
    GTTTTGGAAC GAGTTAGCCG GATGACCCAA GGTGATTTGG TGATGTCAAT GGACCAGCTG
    CTTACCAAAC CCCACCTGGG CACCTGTCAC AAATTTTACA TGCAGATGTT TCAGTTGCCT
    AATGAACAAA CCAAGACACT GATGTTTTTT GAAGGCTGTC CACAGCACCT GGGCTGTACA
    ATCAAGCTTA GAGGAGGCTC TGATTATGAG CTGGCTCGAG TTAAGGAGAT CTTAATATTT
    ATGATCTGTG TAGCTTATCA TTCTCAACTA GAAATCTCCT TCCTTATGGA TGAATTTGCT
    ATGCCTCCTA CATTAACGCA AAACCCTTCC TTCCATTCTC TGATTGAAGG ACAGGAGGAT
    GAAGGGGCTT CACAGGAGCC ATTCAGCGGA AGTCCCCTCC CCCGGGAGCC TGACTTCCCC
    CCAGACTTTC TGCCTTCTGA TGATGGCAGT TTGCTAGAAA CAAGGATTCT GTTTGAGAAG
    GGTGACCAGG AGAATAAGAG TGTGCCACAG GATGTTGCCT CTTTGAAGCC TCAAGAGCAT
    GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC TGTGCTCTCT TTCCATCAGT ACCAGAGTCA
    TTGCTTCCGC TCCATGTGGA TGACCAACAG GACGCCATAG GCAGTGAGCA GCCAGAGGCT
    TTGCAGCAAA CAGAGGAGCT TCCGGATCCC ACAAGCCAGA TGAGAGCCTT TAGAGACCCT
    CTGCAGGATG ACACTGGCCT GTATGTTACC GAGGAAGTTA CCTCTTCTGA AGATAAACGG
    AAGACTGATT CTTTGACCTT TAAGCAAGAG TTGAAAGATG TGATCCTCTG TATCTCGCCA
    GTCATCACAT TCCGGGAGCC CTTCCTTTTA ACTGACAAGG GCATGAGATG CTCTACCCGA
    GATTATTTTG CGGAGCAGGT TTACTGGTCT CCTCTCCTCA ATAAGGAGTT CAAGGAAATG
    GAGAGCAGGC GAAAGAAACA GATGCTTAGG GATCTCTCTG GCCTTCAGGG CATGAATGGA
    AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG CCCTCACATG AGCTCGTGAG CACCAGAATT
    GCTGAGCACC TGGGGGACAG CCAGAGCTTG GGGAGAATGC TAGCCGATTA TCGGGCCAGA
    GGAGGAAGAA TCCAGCAAAA AAACTCAGAC CCTTTTGCTT ACTCAAAGGA GGCATCAAGC
    ACCTCAAGTG GAAAATCAGG AAGCAGAACT GAAGGTGATG AAGAAAAAGG GTTGATTACC
    AGTGATGCAG TGTGGTCGAC AAAGGTGGAC TGTCTGAACC CTGTCAACCA CCAGAGGCTG
    TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG TCCAGCAACG CTCCCAGTGC CTGTGTCAGT
    CCTTGGATTG TAACAATGGA ATTTTATGGA AAAAATGATC TTACATTAGG AATATTTTTA
    GAGAGATACT GTTTCCGGCC TTCCTATCAG TGCCCTAGCA TGTTCTGTGA TACCCCCATG
    GTGCACCACA TTCGACGCTT TGTCCATGGC CAAGGCTGTG TGCAGATAAT CCTAAAGGAG
    CTGGATTCTC CAGTACCTGG ATATCAGCAT ACAATTCTCA CCTACTCCTG GTGCAGAATG
    TGCAAACAGG TAACACCAGT TGTTGCTCTT TCCAATGAGT CTTGGTCCAT GTCGTTTGCA
    AAATACCTGG AACTTAGGTT TTATGGGCAC CAGTACACCC GCAGAGCCAA TGCTGAGCCA
    TGTGGTCACT CCATCCACCA CGATTATCAC CAGTATTTCT CCTATAACCA GATGGTGGCG
    TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT GAAGTGTGTG TTCCACTTCC CAAAATATTC
    ATTAAACGTC AAGTCCCACT AAAGGTGTCC CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG
    AAAGTTTCAC AGGTATATCT TGCTGTGGAT GAAAGACTTG CGTCTTTGAA AACTGATACA
    TTTAGCAAAT CAAGAGAGGA AAAAATGGAA GATATCTTTG CACAAAAAGA GATGGAAGAA
    AGTGAGTTCA AGAGCTGGAC TGAGAAGATG CAGGCGCGGC TCCTGTCTTC CTCCATGGAT
    ACCCCCCAGC AACTGCAGTC AGTCTTCGAG TCCCTCATCG CCAAGAAACA AAGCCTCTGT
    GAGGTGCTGC AAGCTTGGAA TAACAGGCTG CAAGACCTTT TCCAACAAGA AAAGGGTAGG
    AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA AGACTGAGAC AAGGTGAAGA AAGCAAGATA
    AGTGCAGTGG ATGCATCTCC ACGGAATGCT TCTCCAGGAC TTCCAAATGG AGAAAAAGAG
    GACCGCTTCT TAGCCACCCT TTCCAGTCAG AGCTCCACCA GTTCCCCCCA TCTCCAGCTG
    CCTACTTCTC CTGAAGGCGT GCCTGAGCAG GTCATGGGAG GGCCCCCAGA TCTCGATACT
    GCCAGCAGTT CTGAAGATGT ATTTGATGGA CATTTGCTGG GATCTACAGA CAGCCAGGTG
    AAGGAAAAAT CAACCATGAA AGCCATCTTT GCAAATTTGC TTCCAGGAAA TAGCTATAAC
    CCTATTCCAT TTCCTTTCGA TCCAGATAAA CACTACTTAA TGTATGAACA TGAACGAGTG
    CCCATTGCAG TCTGTGAGAA GGAGCCCAGC TCCATCATTG CTTTTGCTCT CAGTTGTAAA
    GAATACCGGA ATGCCTTAGA GGAATTGTCC AAAGCCACTC AGCGGAACAG TGCTGAAGAA
    GGGCTTCCAA CAAACAGTAC TTTAGACAGC AGACCAAAGA GTAGCAGCCC AATTAGACTA
    CCTGAGATCA GTGGAGGACA GACAAACCGT GCGGTAGAAG CAGAACCACA GCCAACCAAA
    AAGGCTTCTG GAATGTTGTC CTTCTTCCGA GGGACAGCAG GGAAAAGCCC TGACCTCTCT
    TCCCAGAAGA GAGAGACCTT ACGTGGAGCA GATAGTGCTT ACTACCAAGT GGGGCAGACG
    GGCAAGGAGG GGACGGAGAA TCAAGGCATT GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT
    ACACAGAAGA AACAACTTAC AAATCCTCAT GTGGAGCTTC AATTTTCGGA TGCCAACGCC
    AAGTTTTACT GTCGGCTCTA CTATGCGGGA GAGTTTCATA AGATGCGAGA AGTGATTCTG
    GGCAGCAGCG AAGAAGATTT CATTCGTTCC CTTTCTCACT CTTCTCCGTG GCAGGCCCGA
    GGAGGCAAAT CAGGAGCTGC CTTTTACGCT ACTGAAGATG ACAGATTCAT TTTGAAGCAA
    ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT GACTTCGCAC CACACTACTT CAATTACATT
    ACAAATGCCG TTCAGCAAAA GAGGCCTACT GCATTGGCCA AGATTCTTGG AGTTTACAGA
    ATTGGTTATA AGAATTCTCA GAACAACACT GAGAAGAAGC TAGATCTCCT TGTCATGGAA
    AATCTTTTCT ACGGGAGAAA GATGGCACAG GTTTTTGACT TGAAGGGTTC ACTTAGGAAT
    CGAAATGTAA AAACCGATAC CGGGAAAGAG AGCTGTGATG TTGTTCTTCT GGATGAAAAT
    CTCTTAAAGA TGGTTCGAGA CAACCCTCTC TATATCCGTT CTCATTCCAA AGCTGTGCTG
    AGAGCCTCGA TCCGGAGCGA CTCCCACTTC CTTTCTAGCC ACCTCATTAT AGACTATTCT
    TTGCTAGTTG GGCGAGACGA TACCAGCAAT GAGCTAGTAG TTGGAATTAT AGATTATATC
    CGAACATTTA CCTGGGACAA AAAGCTTGAG ATGGTTGTGA AATCAACGGG AATTTTAGGA
    GGACAAGGTA AAATGCCGAC TGTGGTCTCT CCAGAGTTGT ACAGGACTAG ATTCTGTGAA
    GCCATGGACA AGTATTTCCT GATGGTGCCA GACCACTGGA CAGGCTTGGG TCTGAACTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834406.1
    CDS155..6397
    Translation

    Target ORF information:

    RefSeq Version XM_024978638.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978638.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGATC AGCCAGCATT ACTAATCTGT CACTGGATCG ATCTGCGTCT
    CCAATGGTAC CATCATACGA GACGTCTGTC AGTCCCCAGG CTAACCGAAC GTATGTTCGG
    ACAGAGACCA CTGAGGATGA ACGCAAAATT CTTCTGGACA GTGTTCAGTT AAAAGACCTA
    TGGAAGAAAA TCTGCCATCA CAGCAGTGGA ATGGAGTTTC AGGATCACCG CTACTGGCTG
    AGAACACACC CCAACTGCAT TGTAGGAAAG GAGTTAGTCA ACTGGTTAAT CCGAAATGGA
    CACATTGCTA CAAGGGCACA GGCTATAGCA ATTGGACAGG CAATGGTGGA TGGACGTTGG
    CTGGATTGTG TCAGTCACCA CGACCAGCTC TTCAGGGATG AGTATGCGCT GTACAGGCCG
    CTGCAGAGTA CAGAATTTTC CGAGACCCCT TCTCCGGACA GTGACTCGGT GAACTCTGTG
    GAAGGACACT CTGAGCCATC CTGGTTTAAA GACATCAAGT TTGATGACAG TGACACTGAG
    CAGATAGCTG AAGAAGGTGA CGATAATTTG ACTAACTCTG CCAGTCCTAG CAAGCGCACA
    TCAGTCAGCA GTTTCCAGTC CACAGTGGAC AGTGACTCAG CCGCTTCCAT CAGCCTGAAC
    GTGGAGCTGG ACAACGTGAA CTTCCATATC AAGAAGCCCT CCAAGTACCC ACATGTGCCC
    CCTCACCCTG CTGACCAAAA AGAGTATTTG ATTTCTGACA ATGGAGGACA ACAGCTATCA
    ATAAGTGACG CCTTCATCAA AGAATCCTTA TTTAACCGTC GAGTTGAGGA AAAATCCAAA
    GAACTGCCTT TCACACCTTT GGGCTGGCAT CATAACAACC TGGAGCTGCT GAGGGAGGAG
    AATGGAGAGA AACAAGCTAT GGAGAGGTTG CTTTCAGCTA ATCATAACCA CATGATGGCG
    CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA TTGTCAACGT CTTGGAGGGA CATCATCGTA
    TCCCTGGTCT GCCAGGTTGT TCAGACAGTC CGACCTGACG TCAAGAACCG GGATGATGAC
    ATGGATATCC GCCAGTTTGT CCACATCAAA AAAATCCCAG GTGGAAAGAA ATTTGATTCT
    GTGGTTGTCA ATGGATTTGT TTGCACCAAG AATATTGCAC ATAAAAAGAT GAATTCTTCT
    ATTAAAAACC CCAAAATTCT TCTGCTGAAA TGTTCCATTG AATATCTCTA CAGAGAAGAA
    ACCAAATTTA CTTGCATTGA TCCTATTGTG CTTCAGGAAA GGGAATTCCT AAAGAATTAT
    GTTCAGCGAA TAGTTGATGT TCGACCCACA CTGGTTCTGG TTGAGAAAAC GGTATCTCGA
    ATTGCACAGG ATATGTTGCT GGAACACGGC ATTACTTTGG TCATTAATGT AAAATCACAA
    GTTTTGGAAC GAGTTAGCCG GATGACCCAA GGTGATTTGG TGATGTCAAT GGACCAGCTG
    CTTACCAAAC CCCACCTGGG CACCTGTCAC AAATTTTACA TGCAGATGTT TCAGTTGCCT
    AATGAACAAA CCAAGACACT GATGTTTTTT GAAGGCTGTC CACAGCACCT GGGCTGTACA
    ATCAAGCTTA GAGGAGGCTC TGATTATGAG CTGGCTCGAG TTAAGGAGAT CTTAATATTT
    ATGATCTGTG TAGCTTATCA TTCTCAACTA GAAATCTCCT TCCTTATGGA TGAATTTGCT
    ATGCCTCCTA CATTAACGCA AAACCCTTCC TTCCATTCTC TGATTGAAGG ACAGGAGGAT
    GAAGGGGCTT CACAGGAGCC ATTCAGCGGA AGTCCCCTCC CCCGGGAGCC TGACTTCCCC
    CCAGACTTTC TGCCTTCTGA TGATGGCAGT TTGCTAGAAA CAAGGATTCT GTTTGAGAAG
    GGTGACCAGG AGAATAAGAG TGTGCCACAG GATGTTGCCT CTTTGAAGCC TCAAGAGCAT
    GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC TGTGCTCTCT TTCCATCAGT ACCAGAGTCA
    TTGCTTCCGC TCCATGTGGA TGACCAACAG GACGCCATAG GCAGTGAGCA GCCAGAGGCT
    TTGCAGCAAA CAGAGGAGCT TCCGGATCCC ACAAGCCAGA TGAGAGCCTT TAGAGACCCT
    CTGCAGGATG ACACTGGCCT GTATGTTACC GAGGAAGTTA CCTCTTCTGA AGATAAACGG
    AAGACTGATT CTTTGACCTT TAAGCAAGAG TTGAAAGATG TGATCCTCTG TATCTCGCCA
    GTCATCACAT TCCGGGAGCC CTTCCTTTTA ACTGACAAGG GCATGAGATG CTCTACCCGA
    GATTATTTTG CGGAGCAGGT TTACTGGTCT CCTCTCCTCA ATAAGGAGTT CAAGGAAATG
    GAGAGCAGGC GAAAGAAACA GATGCTTAGG GATCTCTCTG GCCTTCAGGG CATGAATGGA
    AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG CCCTCACATG AGCTCGTGAG CACCAGAATT
    GCTGAGCACC TGGGGGACAG CCAGAGCTTG GGGAGAATGC TAGCCGATTA TCGGGCCAGA
    GGAGGAAGAA TCCAGCAAAA AAACTCAGAC CCTTTTGCTT ACTCAAAGGA GGCATCAAGC
    ACCTCAAGTG GAAAATCAGG AAGCAGAACT GAAGGTGATG AAGAAAAAGG GTTGATTACC
    AGTGATGCAG TGTGGTCGAC AAAGGTGGAC TGTCTGAACC CTGTCAACCA CCAGAGGCTG
    TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG TCCAGCAACG CTCCCAGTGC CTGTGTCAGT
    CCTTGGATTG TAACAATGGA ATTTTATGGA AAAAATGATC TTACATTAGG AATATTTTTA
    GAGAGATACT GTTTCCGGCC TTCCTATCAG TGCCCTAGCA TGTTCTGTGA TACCCCCATG
    GTGCACCACA TTCGACGCTT TGTCCATGGC CAAGGCTGTG TGCAGATAAT CCTAAAGGAG
    CTGGATTCTC CAGTACCTGG ATATCAGCAT ACAATTCTCA CCTACTCCTG GTGCAGAATG
    TGCAAACAGG TAACACCAGT TGTTGCTCTT TCCAATGAGT CTTGGTCCAT GTCGTTTGCA
    AAATACCTGG AACTTAGGTT TTATGGGCAC CAGTACACCC GCAGAGCCAA TGCTGAGCCA
    TGTGGTCACT CCATCCACCA CGATTATCAC CAGTATTTCT CCTATAACCA GATGGTGGCG
    TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT GAAGTGTGTG TTCCACTTCC CAAAATATTC
    ATTAAACGTC AAGTCCCACT AAAGGTGTCC CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG
    AAAGTTTCAC AGGTATATCT TGCTGTGGAT GAAAGACTTG CGTCTTTGAA AACTGATACA
    TTTAGCAAAT CAAGAGAGGA AAAAATGGAA GATATCTTTG CACAAAAAGA GATGGAAGAA
    AGTGAGTTCA AGAGCTGGAC TGAGAAGATG CAGGCGCGGC TCCTGTCTTC CTCCATGGAT
    ACCCCCCAGC AACTGCAGTC AGTCTTCGAG TCCCTCATCG CCAAGAAACA AAGCCTCTGT
    GAGGTGCTGC AAGCTTGGAA TAACAGGCTG CAAGACCTTT TCCAACAAGA AAAGGGTAGG
    AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA AGACTGAGAC AAGGTGAAGA AAGCAAGATA
    AGTGCAGTGG ATGCATCTCC ACGGAATGCT TCTCCAGGAC TTCCAAATGG AGAAAAAGAG
    GACCGCTTCT TAGCCACCCT TTCCAGTCAG AGCTCCACCA GTTCCCCCCA TCTCCAGCTG
    CCTACTTCTC CTGAAGGCGT GCCTGAGCAG GTCATGGGAG GGCCCCCAGA TCTCGATACT
    GCCAGCAGTT CTGAAGATGT ATTTGATGGA CATTTGCTGG GATCTACAGA CAGCCAGGTG
    AAGGAAAAAT CAACCATGAA AGCCATCTTT GCAAATTTGC TTCCAGGAAA TAGCTATAAC
    CCTATTCCAT TTCCTTTCGA TCCAGATAAA CACTACTTAA TGTATGAACA TGAACGAGTG
    CCCATTGCAG TCTGTGAGAA GGAGCCCAGC TCCATCATTG CTTTTGCTCT CAGTTGTAAA
    GAATACCGGA ATGCCTTAGA GGAATTGTCC AAAGCCACTC AGCGGAACAG TGCTGAAGAA
    GGGCTTCCAA CAAACAGTAC TTTAGACAGC AGACCAAAGA GTAGCAGCCC AATTAGACTA
    CCTGAGATCA GTGGAGGACA GACAAACCGT GCGGTAGAAG CAGAACCACA GCCAACCAAA
    AAGGCTTCTG GAATGTTGTC CTTCTTCCGA GGGACAGCAG GGAAAAGCCC TGACCTCTCT
    TCCCAGAAGA GAGAGACCTT ACGTGGAGCA GATAGTGCTT ACTACCAAGT GGGGCAGACG
    GGCAAGGAGG GGACGGAGAA TCAAGGCATT GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT
    ACACAGAAGA AACAACTTAC AAATCCTCAT GTGGAGCTTC AATTTTCGGA TGCCAACGCC
    AAGTTTTACT GTCGGCTCTA CTATGCGGGA GAGTTTCATA AGATGCGAGA AGTGATTCTG
    GGCAGCAGCG AAGAAGATTT CATTCGTTCC CTTTCTCACT CTTCTCCGTG GCAGGCCCGA
    GGAGGCAAAT CAGGAGCTGC CTTTTACGCT ACTGAAGATG ACAGATTCAT TTTGAAGCAA
    ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT GACTTCGCAC CACACTACTT CAATTACATT
    ACAAATGCCG TTCAGCAAAA GAGGCCTACT GCATTGGCCA AGATTCTTGG AGTTTACAGA
    ATTGGTTATA AGAATTCTCA GAACAACACT GAGAAGAAGC TAGATCTCCT TGTCATGGAA
    AATCTTTTCT ACGGGAGAAA GATGGCACAG GTTTTTGACT TGAAGGGTTC ACTTAGGAAT
    CGAAATGTAA AAACCGATAC CGGGAAAGAG AGCTGTGATG TTGTTCTTCT GGATGAAAAT
    CTCTTAAAGA TGGTTCGAGA CAACCCTCTC TATATCCGTT CTCATTCCAA AGCTGTGCTG
    AGAGCCTCGA TCCGGAGCGA CTCCCACTTC CTTTCTAGCC ACCTCATTAT AGACTATTCT
    TTGCTAGTTG GGCGAGACGA TACCAGCAAT GAGCTAGTAG TTGGAATTAT AGATTATATC
    CGAACATTTA CCTGGGACAA AAAGCTTGAG ATGGTTGTGA AATCAACGGG AATTTTAGGA
    GGACAAGGTA AAATGCCGAC TGTGGTCTCT CCAGAGTTGT ACAGGACTAG ATTCTGTGAA
    GCCATGGACA AGTATTTCCT GATGGTGCCA GACCACTGGA CAGGCTTGGG TCTGAACTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227597
    Clone ID Related Accession (Same CDS sequence) XM_024978640.1
    Accession Version XM_024978640.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGCAAG TTTTGGAACG AGTTAGCCGG ATGACCCAAG GTGATTTGGT GATGTCAATG
    GACCAGCTGC TTACCAAACC CCACCTGGGC ACCTGTCACA AATTTTACAT GCAGATGTTT
    CAGTTGCCTA ATGAACAAAC CAAGACACTG ATGTTTTTTG AAGGCTGTCC ACAGCACCTG
    GGCTGTACAA TCAAGCTTAG AGGAGGCTCT GATTATGAGC TGGCTCGAGT TAAGGAGATC
    TTAATATTTA TGATCTGTGT AGCTTATCAT TCTCAACTAG AAATCTCCTT CCTTATGGAT
    GAATTTGCTA TGCCTCCTAC ATTAACGCAA AACCCTTCCT TCCATTCTCT GATTGAAGGA
    CAGGAGGATG AAGGGGCTTC ACAGGAGCCA TTCAGCGGAA GTCCCCTCCC CCGGGAGCCT
    GACTTCCCCC CAGACTTTCT GCCTTCTGAT GATGGCAGTT TGCTAGAAAC AAGGATTCTG
    TTTGAGAAGG GTGACCAGGA GAATAAGAGT GTGCCACAGG ATGTTGCCTC TTTGAAGCCT
    CAAGAGCATG CCCCAGCAGC TTGCCCGATG GGTGCCCCCT GTGCTCTCTT TCCATCAGTA
    CCAGAGTCAT TGCTTCCGCT CCATGTGGAT GACCAACAGG ACGCCATAGG CAGTGAGCAG
    CCAGAGGCTT TGCAGCAAAC AGAGGAGCTT CCGGATCCCA CAAGCCAGAT GAGAGCCTTT
    AGAGACCCTC TGCAGGATGA CACTGGCCTG TATGTTACCG AGGAAGTTAC CTCTTCTGAA
    GATAAACGGA AGACTGATTC TTTGACCTTT AAGCAAGAGT TGAAAGATGT GATCCTCTGT
    ATCTCGCCAG TCATCACATT CCGGGAGCCC TTCCTTTTAA CTGACAAGGG CATGAGATGC
    TCTACCCGAG ATTATTTTGC GGAGCAGGTT TACTGGTCTC CTCTCCTCAA TAAGGAGTTC
    AAGGAAATGG AGAGCAGGCG AAAGAAACAG ATGCTTAGGG ATCTCTCTGG CCTTCAGGGC
    ATGAATGGAA GTGTTCAGGC CAAGTCCATT CAAGTCCTGC CCTCACATGA GCTCGTGAGC
    ACCAGAATTG CTGAGCACCT GGGGGACAGC CAGAGCTTGG GGAGAATGCT AGCCGATTAT
    CGGGCCAGAG GAGGAAGAAT CCAGCAAAAA AACTCAGACC CTTTTGCTTA CTCAAAGGAG
    GCATCAAGCA CCTCAAGTGG AAAATCAGGA AGCAGAACTG AAGGTGATGA AGAAAAAGGG
    TTGATTACCA GTGATGCAGT GTGGTCGACA AAGGTGGACT GTCTGAACCC TGTCAACCAC
    CAGAGGCTGT GTGTGCTCTT CAGCAGCTCT TCTGCCCAGT CCAGCAACGC TCCCAGTGCC
    TGTGTCAGTC CTTGGATTGT AACAATGGAA TTTTATGGAA AAAATGATCT TACATTAGGA
    ATATTTTTAG AGAGATACTG TTTCCGGCCT TCCTATCAGT GCCCTAGCAT GTTCTGTGAT
    ACCCCCATGG TGCACCACAT TCGACGCTTT GTCCATGGCC AAGGCTGTGT GCAGATAATC
    CTAAAGGAGC TGGATTCTCC AGTACCTGGA TATCAGCATA CAATTCTCAC CTACTCCTGG
    TGCAGAATGT GCAAACAGGT AACACCAGTT GTTGCTCTTT CCAATGAGTC TTGGTCCATG
    TCGTTTGCAA AATACCTGGA ACTTAGGTTT TATGGGCACC AGTACACCCG CAGAGCCAAT
    GCTGAGCCAT GTGGTCACTC CATCCACCAC GATTATCACC AGTATTTCTC CTATAACCAG
    ATGGTGGCGT CTTTCAGTTA TTCTCCCATT CGGCTTCTTG AAGTGTGTGT TCCACTTCCC
    AAAATATTCA TTAAACGTCA AGTCCCACTA AAGGTGTCCC TTCTTCAGGA TCTGAAGGAC
    TTCTTTCAGA AAGTTTCACA GGTATATCTT GCTGTGGATG AAAGACTTGC GTCTTTGAAA
    ACTGATACAT TTAGCAAATC AAGAGAGGAA AAAATGGAAG ATATCTTTGC ACAAAAAGAG
    ATGGAAGAAA GTGAGTTCAA GAGCTGGACT GAGAAGATGC AGGCGCGGCT CCTGTCTTCC
    TCCATGGATA CCCCCCAGCA ACTGCAGTCA GTCTTCGAGT CCCTCATCGC CAAGAAACAA
    AGCCTCTGTG AGGTGCTGCA AGCTTGGAAT AACAGGCTGC AAGACCTTTT CCAACAAGAA
    AAGGGTAGGA AGCGGCCTTC AGTTCCTCCA AGTCCTGGAA GACTGAGACA AGGTGAAGAA
    AGCAAGATAA GTGCAGTGGA TGCATCTCCA CGGAATGCTT CTCCAGGACT TCCAAATGGA
    GAAAAAGAGG ACCGCTTCTT AGCCACCCTT TCCAGTCAGA GCTCCACCAG TTCCCCCCAT
    CTCCAGCTGC CTACTTCTCC TGAAGGCGTG CCTGAGCAGG TCATGGGAGG GCCCCCAGAT
    CTCGATACTG CCAGCAGTTC TGAAGATGTA TTTGATGGAC ATTTGCTGGG ATCTACAGAC
    AGCCAGGTGA AGGAAAAATC AACCATGAAA GCCATCTTTG CAAATTTGCT TCCAGGAAAT
    AGCTATAACC CTATTCCATT TCCTTTCGAT CCAGATAAAC ACTACTTAAT GTATGAACAT
    GAACGAGTGC CCATTGCAGT CTGTGAGAAG GAGCCCAGCT CCATCATTGC TTTTGCTCTC
    AGTTGTAAAG AATACCGGAA TGCCTTAGAG GAATTGTCCA AAGCCACTCA GCGGAACAGT
    GCTGAAGAAG GGCTTCCAAC AAACAGTACT TTAGACAGCA GACCAAAGAG TAGCAGCCCA
    ATTAGACTAC CTGAGATCAG TGGAGGACAG ACAAACCGTG CGGTAGAAGC AGAACCACAG
    CCAACCAAAA AGGCTTCTGG AATGTTGTCC TTCTTCCGAG GGACAGCAGG GAAAAGCCCT
    GACCTCTCTT CCCAGAAGAG AGAGACCTTA CGTGGAGCAG ATAGTGCTTA CTACCAAGTG
    GGGCAGACGG GCAAGGAGGG GACGGAGAAT CAAGGCATTG AGCCTCAAGA TGAAGTAGAT
    GGAGGAGATA CACAGAAGAA ACAACTTACA AATCCTCATG TGGAGCTTCA ATTTTCGGAT
    GCCAACGCCA AGTTTTACTG TCGGCTCTAC TATGCGGGAG AGTTTCATAA GATGCGAGAA
    GTGATTCTGG GCAGCAGCGA AGAAGATTTC ATTCGTTCCC TTTCTCACTC TTCTCCGTGG
    CAGGCCCGAG GAGGCAAATC AGGAGCTGCC TTTTACGCTA CTGAAGATGA CAGATTCATT
    TTGAAGCAAA TGCCTCGTCT GGAAGTCCAG TCTTTCCTTG ACTTCGCACC ACACTACTTC
    AATTACATTA CAAATGCCGT TCAGCAAAAG AGGCCTACTG CATTGGCCAA GATTCTTGGA
    GTTTACAGAA TTGGTTATAA GAATTCTCAG AACAACACTG AGAAGAAGCT AGATCTCCTT
    GTCATGGAAA ATCTTTTCTA CGGGAGAAAG ATGGCACAGG TTTTTGACTT GAAGGGTTCA
    CTTAGGAATC GAAATGTAAA AACCGATACC GGGAAAGAGA GCTGTGATGT TGTTCTTCTG
    GATGAAAATC TCTTAAAGAT GGTTCGAGAC AACCCTCTCT ATATCCGTTC TCATTCCAAA
    GCTGTGCTGA GAGCCTCGAT CCGGAGCGAC TCCCACTTCC TTTCTAGCCA CCTCATTATA
    GACTATTCTT TGCTAGTTGG GCGAGACGAT ACCAGCAATG AGCTAGTAGT TGGAATTATA
    GATTATATCC GAACATTTAC CTGGGACAAA AAGCTTGAGA TGGTTGTGAA ATCAACGGGA
    ATTTTAGGAG GACAAGGTAA AATGCCGACT GTGGTCTCTC CAGAGTTGTA CAGGACTAGA
    TTCTGTGAAG CCATGGACAA GTATTTCCTG ATGGTGCCAG ACCACTGGAC AGGCTTGGGT
    CTGAACTGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834408.1
    CDS155..6346
    Translation

    Target ORF information:

    RefSeq Version XM_024978640.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978640.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGCAAG TTTTGGAACG AGTTAGCCGG ATGACCCAAG GTGATTTGGT GATGTCAATG
    GACCAGCTGC TTACCAAACC CCACCTGGGC ACCTGTCACA AATTTTACAT GCAGATGTTT
    CAGTTGCCTA ATGAACAAAC CAAGACACTG ATGTTTTTTG AAGGCTGTCC ACAGCACCTG
    GGCTGTACAA TCAAGCTTAG AGGAGGCTCT GATTATGAGC TGGCTCGAGT TAAGGAGATC
    TTAATATTTA TGATCTGTGT AGCTTATCAT TCTCAACTAG AAATCTCCTT CCTTATGGAT
    GAATTTGCTA TGCCTCCTAC ATTAACGCAA AACCCTTCCT TCCATTCTCT GATTGAAGGA
    CAGGAGGATG AAGGGGCTTC ACAGGAGCCA TTCAGCGGAA GTCCCCTCCC CCGGGAGCCT
    GACTTCCCCC CAGACTTTCT GCCTTCTGAT GATGGCAGTT TGCTAGAAAC AAGGATTCTG
    TTTGAGAAGG GTGACCAGGA GAATAAGAGT GTGCCACAGG ATGTTGCCTC TTTGAAGCCT
    CAAGAGCATG CCCCAGCAGC TTGCCCGATG GGTGCCCCCT GTGCTCTCTT TCCATCAGTA
    CCAGAGTCAT TGCTTCCGCT CCATGTGGAT GACCAACAGG ACGCCATAGG CAGTGAGCAG
    CCAGAGGCTT TGCAGCAAAC AGAGGAGCTT CCGGATCCCA CAAGCCAGAT GAGAGCCTTT
    AGAGACCCTC TGCAGGATGA CACTGGCCTG TATGTTACCG AGGAAGTTAC CTCTTCTGAA
    GATAAACGGA AGACTGATTC TTTGACCTTT AAGCAAGAGT TGAAAGATGT GATCCTCTGT
    ATCTCGCCAG TCATCACATT CCGGGAGCCC TTCCTTTTAA CTGACAAGGG CATGAGATGC
    TCTACCCGAG ATTATTTTGC GGAGCAGGTT TACTGGTCTC CTCTCCTCAA TAAGGAGTTC
    AAGGAAATGG AGAGCAGGCG AAAGAAACAG ATGCTTAGGG ATCTCTCTGG CCTTCAGGGC
    ATGAATGGAA GTGTTCAGGC CAAGTCCATT CAAGTCCTGC CCTCACATGA GCTCGTGAGC
    ACCAGAATTG CTGAGCACCT GGGGGACAGC CAGAGCTTGG GGAGAATGCT AGCCGATTAT
    CGGGCCAGAG GAGGAAGAAT CCAGCAAAAA AACTCAGACC CTTTTGCTTA CTCAAAGGAG
    GCATCAAGCA CCTCAAGTGG AAAATCAGGA AGCAGAACTG AAGGTGATGA AGAAAAAGGG
    TTGATTACCA GTGATGCAGT GTGGTCGACA AAGGTGGACT GTCTGAACCC TGTCAACCAC
    CAGAGGCTGT GTGTGCTCTT CAGCAGCTCT TCTGCCCAGT CCAGCAACGC TCCCAGTGCC
    TGTGTCAGTC CTTGGATTGT AACAATGGAA TTTTATGGAA AAAATGATCT TACATTAGGA
    ATATTTTTAG AGAGATACTG TTTCCGGCCT TCCTATCAGT GCCCTAGCAT GTTCTGTGAT
    ACCCCCATGG TGCACCACAT TCGACGCTTT GTCCATGGCC AAGGCTGTGT GCAGATAATC
    CTAAAGGAGC TGGATTCTCC AGTACCTGGA TATCAGCATA CAATTCTCAC CTACTCCTGG
    TGCAGAATGT GCAAACAGGT AACACCAGTT GTTGCTCTTT CCAATGAGTC TTGGTCCATG
    TCGTTTGCAA AATACCTGGA ACTTAGGTTT TATGGGCACC AGTACACCCG CAGAGCCAAT
    GCTGAGCCAT GTGGTCACTC CATCCACCAC GATTATCACC AGTATTTCTC CTATAACCAG
    ATGGTGGCGT CTTTCAGTTA TTCTCCCATT CGGCTTCTTG AAGTGTGTGT TCCACTTCCC
    AAAATATTCA TTAAACGTCA AGTCCCACTA AAGGTGTCCC TTCTTCAGGA TCTGAAGGAC
    TTCTTTCAGA AAGTTTCACA GGTATATCTT GCTGTGGATG AAAGACTTGC GTCTTTGAAA
    ACTGATACAT TTAGCAAATC AAGAGAGGAA AAAATGGAAG ATATCTTTGC ACAAAAAGAG
    ATGGAAGAAA GTGAGTTCAA GAGCTGGACT GAGAAGATGC AGGCGCGGCT CCTGTCTTCC
    TCCATGGATA CCCCCCAGCA ACTGCAGTCA GTCTTCGAGT CCCTCATCGC CAAGAAACAA
    AGCCTCTGTG AGGTGCTGCA AGCTTGGAAT AACAGGCTGC AAGACCTTTT CCAACAAGAA
    AAGGGTAGGA AGCGGCCTTC AGTTCCTCCA AGTCCTGGAA GACTGAGACA AGGTGAAGAA
    AGCAAGATAA GTGCAGTGGA TGCATCTCCA CGGAATGCTT CTCCAGGACT TCCAAATGGA
    GAAAAAGAGG ACCGCTTCTT AGCCACCCTT TCCAGTCAGA GCTCCACCAG TTCCCCCCAT
    CTCCAGCTGC CTACTTCTCC TGAAGGCGTG CCTGAGCAGG TCATGGGAGG GCCCCCAGAT
    CTCGATACTG CCAGCAGTTC TGAAGATGTA TTTGATGGAC ATTTGCTGGG ATCTACAGAC
    AGCCAGGTGA AGGAAAAATC AACCATGAAA GCCATCTTTG CAAATTTGCT TCCAGGAAAT
    AGCTATAACC CTATTCCATT TCCTTTCGAT CCAGATAAAC ACTACTTAAT GTATGAACAT
    GAACGAGTGC CCATTGCAGT CTGTGAGAAG GAGCCCAGCT CCATCATTGC TTTTGCTCTC
    AGTTGTAAAG AATACCGGAA TGCCTTAGAG GAATTGTCCA AAGCCACTCA GCGGAACAGT
    GCTGAAGAAG GGCTTCCAAC AAACAGTACT TTAGACAGCA GACCAAAGAG TAGCAGCCCA
    ATTAGACTAC CTGAGATCAG TGGAGGACAG ACAAACCGTG CGGTAGAAGC AGAACCACAG
    CCAACCAAAA AGGCTTCTGG AATGTTGTCC TTCTTCCGAG GGACAGCAGG GAAAAGCCCT
    GACCTCTCTT CCCAGAAGAG AGAGACCTTA CGTGGAGCAG ATAGTGCTTA CTACCAAGTG
    GGGCAGACGG GCAAGGAGGG GACGGAGAAT CAAGGCATTG AGCCTCAAGA TGAAGTAGAT
    GGAGGAGATA CACAGAAGAA ACAACTTACA AATCCTCATG TGGAGCTTCA ATTTTCGGAT
    GCCAACGCCA AGTTTTACTG TCGGCTCTAC TATGCGGGAG AGTTTCATAA GATGCGAGAA
    GTGATTCTGG GCAGCAGCGA AGAAGATTTC ATTCGTTCCC TTTCTCACTC TTCTCCGTGG
    CAGGCCCGAG GAGGCAAATC AGGAGCTGCC TTTTACGCTA CTGAAGATGA CAGATTCATT
    TTGAAGCAAA TGCCTCGTCT GGAAGTCCAG TCTTTCCTTG ACTTCGCACC ACACTACTTC
    AATTACATTA CAAATGCCGT TCAGCAAAAG AGGCCTACTG CATTGGCCAA GATTCTTGGA
    GTTTACAGAA TTGGTTATAA GAATTCTCAG AACAACACTG AGAAGAAGCT AGATCTCCTT
    GTCATGGAAA ATCTTTTCTA CGGGAGAAAG ATGGCACAGG TTTTTGACTT GAAGGGTTCA
    CTTAGGAATC GAAATGTAAA AACCGATACC GGGAAAGAGA GCTGTGATGT TGTTCTTCTG
    GATGAAAATC TCTTAAAGAT GGTTCGAGAC AACCCTCTCT ATATCCGTTC TCATTCCAAA
    GCTGTGCTGA GAGCCTCGAT CCGGAGCGAC TCCCACTTCC TTTCTAGCCA CCTCATTATA
    GACTATTCTT TGCTAGTTGG GCGAGACGAT ACCAGCAATG AGCTAGTAGT TGGAATTATA
    GATTATATCC GAACATTTAC CTGGGACAAA AAGCTTGAGA TGGTTGTGAA ATCAACGGGA
    ATTTTAGGAG GACAAGGTAA AATGCCGACT GTGGTCTCTC CAGAGTTGTA CAGGACTAGA
    TTCTGTGAAG CCATGGACAA GTATTTCCTG ATGGTGCCAG ACCACTGGAC AGGCTTGGGT
    CTGAACTGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16660
    Clone ID Related Accession (Same CDS sequence) XM_005202762.4 , XM_005202762.3 , XM_024978630.1 , NM_001205467.1
    Accession Version NM_001205467.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6297bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000002.1. On Apr 20, 2011 this sequence version replaced XM_001257235.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145432, SAMN03145448 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGACACCAGA AGGAAGGCAG AACCTACTTT TGGAGGTCAT
    GACCCTCGCA CAGCTGTTCA ACTCCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA
    ATCATGGAGG GGAAGAGCCA GGATAGTGAC CTGAAGCAAT ACTGGATGCC AGATAGCCAG
    TGTAAAGAGT GCTATGACTG TAATGAGAAA TTTACCACTT TTAGGCGCAG ACACCACTGC
    CGACTCTGTG GGCAGATTTT CTGCAGTCGT TGCTGCAATC AAGAAATCCC TGGAAAATTT
    ATGGGTTATA CAGGGGACCT CCGAGCCTGT ACATACTGTA GAAAAATAGC CTTAAGTTAT
    GCTCATTCCA CAGACAGTAA TTCCATTGGT GAAGACTTGA ATGCTCTTTC AGACTCTGCT
    TCCTCTGTGT CTGTACTTGA TCCAAATGAA CCCCGTACAC CTGTGGGGAG TAGAAAAGCC
    AGCCGCAACA TATTTTTAGA GGATGATTTT GCCTGGCAAA GTTTAATTCA TCCAGACTCC
    TCAAACACTG CTCTTTCAAC AAGACTTGTA TCTGTTCAAG AGGATGCTGG GAAATCTCCT
    GCTCGAAATA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGATCTGC GTCTCCAATG
    GTACCATCAT ACGAGACGTC TGTCAGTCCC CAGGCTAACC GAACGTATGT TCGGACAGAG
    ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTTC AGTTAAAAGA CCTATGGAAG
    AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GCTGAGAACA
    CACCCCAACT GCATTGTAGG AAAGGAGTTA GTCAACTGGT TAATCCGAAA TGGACACATT
    GCTACAAGGG CACAGGCTAT AGCAATTGGA CAGGCAATGG TGGATGGACG TTGGCTGGAT
    TGTGTCAGTC ACCACGACCA GCTCTTCAGG GATGAGTATG CGCTGTACAG GCCGCTGCAG
    AGTACAGAAT TTTCCGAGAC CCCTTCTCCG GACAGTGACT CGGTGAACTC TGTGGAAGGA
    CACTCTGAGC CATCCTGGTT TAAAGACATC AAGTTTGATG ACAGTGACAC TGAGCAGATA
    GCTGAAGAAG GTGACGATAA TTTGACTAAC TCTGCCAGTC CTAGCAAGCG CACATCAGTC
    AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CCATCAGCCT GAACGTGGAG
    CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC
    CCTGCTGACC AAAAAGAGTA TTTGATTTCT GACAATGGAG GACAACAGCT ATCAATAAGT
    GACGCCTTCA TCAAAGAATC CTTATTTAAC CGTCGAGTTG AGGAAAAATC CAAAGAACTG
    CCTTTCACAC CTTTGGGCTG GCATCATAAC AACCTGGAGC TGCTGAGGGA GGAGAATGGA
    GAGAAACAAG CTATGGAGAG GTTGCTTTCA GCTAATCATA ACCACATGAT GGCGCTGCTC
    CAACAGTTGC TCCATAGTGA GTCATTGTCA ACGTCTTGGA GGGACATCAT CGTATCCCTG
    GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGA ACCGGGATGA TGACATGGAT
    ATCCGCCAGT TTGTCCACAT CAAAAAAATC CCAGGTGGAA AGAAATTTGA TTCTGTGGTT
    GTCAATGGAT TTGTTTGCAC CAAGAATATT GCACATAAAA AGATGAATTC TTCTATTAAA
    AACCCCAAAA TTCTTCTGCT GAAATGTTCC ATTGAATATC TCTACAGAGA AGAAACCAAA
    TTTACTTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCCTAAAGAA TTATGTTCAG
    CGAATAGTTG ATGTTCGACC CACACTGGTT CTGGTTGAGA AAACGGTATC TCGAATTGCA
    CAGGATATGT TGCTGGAACA CGGCATTACT TTGGTCATTA ATGTAAAATC ACAAGTTTTG
    GAACGAGTTA GCCGGATGAC CCAAGGTGAT TTGGTGATGT CAATGGACCA GCTGCTTACC
    AAACCCCACC TGGGCACCTG TCACAAATTT TACATGCAGA TGTTTCAGTT GCCTAATGAA
    CAAACCAAGA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTGGGCTG TACAATCAAG
    CTTAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCTTAAT ATTTATGATC
    TGTGTAGCTT ATCATTCTCA ACTAGAAATC TCCTTCCTTA TGGATGAATT TGCTATGCCT
    CCTACATTAA CGCAAAACCC TTCCTTCCAT TCTCTGATTG AAGGACAGGA GGATGAAGGG
    GCTTCACAGG AGCCATTCAG CGGAAGTCCC CTCCCCCGGG AGCCTGACTT CCCCCCAGAC
    TTTCTGCCTT CTGATGATGG CAGTTTGCTA GAAACAAGGA TTCTGTTTGA GAAGGGTGAC
    CAGGAGAATA AGAGTGTGCC ACAGGATGTT GCCTCTTTGA AGCCTCAAGA GCATGCCCCA
    GCAGCTTGCC CGATGGGTGC CCCCTGTGCT CTCTTTCCAT CAGTACCAGA GTCATTGCTT
    CCGCTCCATG TGGATGACCA ACAGGACGCC ATAGGCAGTG AGCAGCCAGA GGCTTTGCAG
    CAAACAGAGG AGCTTCCGGA TCCCACAAGC CAGATGAGAG CCTTTAGAGA CCCTCTGCAG
    GATGACACTG GCCTGTATGT TACCGAGGAA GTTACCTCTT CTGAAGATAA ACGGAAGACT
    GATTCTTTGA CCTTTAAGCA AGAGTTGAAA GATGTGATCC TCTGTATCTC GCCAGTCATC
    ACATTCCGGG AGCCCTTCCT TTTAACTGAC AAGGGCATGA GATGCTCTAC CCGAGATTAT
    TTTGCGGAGC AGGTTTACTG GTCTCCTCTC CTCAATAAGG AGTTCAAGGA AATGGAGAGC
    AGGCGAAAGA AACAGATGCT TAGGGATCTC TCTGGCCTTC AGGGCATGAA TGGAAGTGTT
    CAGGCCAAGT CCATTCAAGT CCTGCCCTCA CATGAGCTCG TGAGCACCAG AATTGCTGAG
    CACCTGGGGG ACAGCCAGAG CTTGGGGAGA ATGCTAGCCG ATTATCGGGC CAGAGGAGGA
    AGAATCCAGC AAAAAAACTC AGACCCTTTT GCTTACTCAA AGGAGGCATC AAGCACCTCA
    AGTGGAAAAT CAGGAAGCAG AACTGAAGGT GATGAAGAAA AAGGGTTGAT TACCAGTGAT
    GCAGTGTGGT CGACAAAGGT GGACTGTCTG AACCCTGTCA ACCACCAGAG GCTGTGTGTG
    CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC AACGCTCCCA GTGCCTGTGT CAGTCCTTGG
    ATTGTAACAA TGGAATTTTA TGGAAAAAAT GATCTTACAT TAGGAATATT TTTAGAGAGA
    TACTGTTTCC GGCCTTCCTA TCAGTGCCCT AGCATGTTCT GTGATACCCC CATGGTGCAC
    CACATTCGAC GCTTTGTCCA TGGCCAAGGC TGTGTGCAGA TAATCCTAAA GGAGCTGGAT
    TCTCCAGTAC CTGGATATCA GCATACAATT CTCACCTACT CCTGGTGCAG AATGTGCAAA
    CAGGTAACAC CAGTTGTTGC TCTTTCCAAT GAGTCTTGGT CCATGTCGTT TGCAAAATAC
    CTGGAACTTA GGTTTTATGG GCACCAGTAC ACCCGCAGAG CCAATGCTGA GCCATGTGGT
    CACTCCATCC ACCACGATTA TCACCAGTAT TTCTCCTATA ACCAGATGGT GGCGTCTTTC
    AGTTATTCTC CCATTCGGCT TCTTGAAGTG TGTGTTCCAC TTCCCAAAAT ATTCATTAAA
    CGTCAAGTCC CACTAAAGGT GTCCCTTCTT CAGGATCTGA AGGACTTCTT TCAGAAAGTT
    TCACAGGTAT ATCTTGCTGT GGATGAAAGA CTTGCGTCTT TGAAAACTGA TACATTTAGC
    AAATCAAGAG AGGAAAAAAT GGAAGATATC TTTGCACAAA AAGAGATGGA AGAAAGTGAG
    TTCAAGAGCT GGACTGAGAA GATGCAGGCG CGGCTCCTGT CTTCCTCCAT GGATACCCCC
    CAGCAACTGC AGTCAGTCTT CGAGTCCCTC ATCGCCAAGA AACAAAGCCT CTGTGAGGTG
    CTGCAAGCTT GGAATAACAG GCTGCAAGAC CTTTTCCAAC AAGAAAAGGG TAGGAAGCGG
    CCTTCAGTTC CTCCAAGTCC TGGAAGACTG AGACAAGGTG AAGAAAGCAA GATAAGTGCA
    GTGGATGCAT CTCCACGGAA TGCTTCTCCA GGACTTCCAA ATGGAGAAAA AGAGGACCGC
    TTCTTAGCCA CCCTTTCCAG TCAGAGCTCC ACCAGTTCCC CCCATCTCCA GCTGCCTACT
    TCTCCTGAAG GCGTGCCTGA GCAGGTCATG GGAGGGCCCC CAGATCTCGA TACTGCCAGC
    AGTTCTGAAG ATGTATTTGA TGGACATTTG CTGGGATCTA CAGACAGCCA GGTGAAGGAA
    AAATCAACCA TGAAAGCCAT CTTTGCAAAT TTGCTTCCAG GAAATAGCTA TAACCCTATT
    CCATTTCCTT TCGATCCAGA TAAACACTAC TTAATGTATG AACATGAACG AGTGCCCATT
    GCAGTCTGTG AGAAGGAGCC CAGCTCCATC ATTGCTTTTG CTCTCAGTTG TAAAGAATAC
    CGGAATGCCT TAGAGGAATT GTCCAAAGCC ACTCAGCGGA ACAGTGCTGA AGAAGGGCTT
    CCAACAAACA GTACTTTAGA CAGCAGACCA AAGAGTAGCA GCCCAATTAG ACTACCTGAG
    ATCAGTGGAG GACAGACAAA CCGTGCGGTA GAAGCAGAAC CACAGCCAAC CAAAAAGGCT
    TCTGGAATGT TGTCCTTCTT CCGAGGGACA GCAGGGAAAA GCCCTGACCT CTCTTCCCAG
    AAGAGAGAGA CCTTACGTGG AGCAGATAGT GCTTACTACC AAGTGGGGCA GACGGGCAAG
    GAGGGGACGG AGAATCAAGG CATTGAGCCT CAAGATGAAG TAGATGGAGG AGATACACAG
    AAGAAACAAC TTACAAATCC TCATGTGGAG CTTCAATTTT CGGATGCCAA CGCCAAGTTT
    TACTGTCGGC TCTACTATGC GGGAGAGTTT CATAAGATGC GAGAAGTGAT TCTGGGCAGC
    AGCGAAGAAG ATTTCATTCG TTCCCTTTCT CACTCTTCTC CGTGGCAGGC CCGAGGAGGC
    AAATCAGGAG CTGCCTTTTA CGCTACTGAA GATGACAGAT TCATTTTGAA GCAAATGCCT
    CGTCTGGAAG TCCAGTCTTT CCTTGACTTC GCACCACACT ACTTCAATTA CATTACAAAT
    GCCGTTCAGC AAAAGAGGCC TACTGCATTG GCCAAGATTC TTGGAGTTTA CAGAATTGGT
    TATAAGAATT CTCAGAACAA CACTGAGAAG AAGCTAGATC TCCTTGTCAT GGAAAATCTT
    TTCTACGGGA GAAAGATGGC ACAGGTTTTT GACTTGAAGG GTTCACTTAG GAATCGAAAT
    GTAAAAACCG ATACCGGGAA AGAGAGCTGT GATGTTGTTC TTCTGGATGA AAATCTCTTA
    AAGATGGTTC GAGACAACCC TCTCTATATC CGTTCTCATT CCAAAGCTGT GCTGAGAGCC
    TCGATCCGGA GCGACTCCCA CTTCCTTTCT AGCCACCTCA TTATAGACTA TTCTTTGCTA
    GTTGGGCGAG ACGATACCAG CAATGAGCTA GTAGTTGGAA TTATAGATTA TATCCGAACA
    TTTACCTGGG ACAAAAAGCT TGAGATGGTT GTGAAATCAA CGGGAATTTT AGGAGGACAA
    GGTAAAATGC CGACTGTGGT CTCTCCAGAG TTGTACAGGA CTAGATTCTG TGAAGCCATG
    GACAAGTATT TCCTGATGGT GCCAGACCAC TGGACAGGCT TGGGTCTGAA CTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192396.1
    CDS1..6297
    Translation

    Target ORF information:

    RefSeq Version NM_001205467.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205467.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGACACCAGA AGGAAGGCAG AACCTACTTT TGGAGGTCAT
    GACCCTCGCA CAGCTGTTCA ACTCCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA
    ATCATGGAGG GGAAGAGCCA GGATAGTGAC CTGAAGCAAT ACTGGATGCC AGATAGCCAG
    TGTAAAGAGT GCTATGACTG TAATGAGAAA TTTACCACTT TTAGGCGCAG ACACCACTGC
    CGACTCTGTG GGCAGATTTT CTGCAGTCGT TGCTGCAATC AAGAAATCCC TGGAAAATTT
    ATGGGTTATA CAGGGGACCT CCGAGCCTGT ACATACTGTA GAAAAATAGC CTTAAGTTAT
    GCTCATTCCA CAGACAGTAA TTCCATTGGT GAAGACTTGA ATGCTCTTTC AGACTCTGCT
    TCCTCTGTGT CTGTACTTGA TCCAAATGAA CCCCGTACAC CTGTGGGGAG TAGAAAAGCC
    AGCCGCAACA TATTTTTAGA GGATGATTTT GCCTGGCAAA GTTTAATTCA TCCAGACTCC
    TCAAACACTG CTCTTTCAAC AAGACTTGTA TCTGTTCAAG AGGATGCTGG GAAATCTCCT
    GCTCGAAATA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGATCTGC GTCTCCAATG
    GTACCATCAT ACGAGACGTC TGTCAGTCCC CAGGCTAACC GAACGTATGT TCGGACAGAG
    ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTTC AGTTAAAAGA CCTATGGAAG
    AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GCTGAGAACA
    CACCCCAACT GCATTGTAGG AAAGGAGTTA GTCAACTGGT TAATCCGAAA TGGACACATT
    GCTACAAGGG CACAGGCTAT AGCAATTGGA CAGGCAATGG TGGATGGACG TTGGCTGGAT
    TGTGTCAGTC ACCACGACCA GCTCTTCAGG GATGAGTATG CGCTGTACAG GCCGCTGCAG
    AGTACAGAAT TTTCCGAGAC CCCTTCTCCG GACAGTGACT CGGTGAACTC TGTGGAAGGA
    CACTCTGAGC CATCCTGGTT TAAAGACATC AAGTTTGATG ACAGTGACAC TGAGCAGATA
    GCTGAAGAAG GTGACGATAA TTTGACTAAC TCTGCCAGTC CTAGCAAGCG CACATCAGTC
    AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CCATCAGCCT GAACGTGGAG
    CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC
    CCTGCTGACC AAAAAGAGTA TTTGATTTCT GACAATGGAG GACAACAGCT ATCAATAAGT
    GACGCCTTCA TCAAAGAATC CTTATTTAAC CGTCGAGTTG AGGAAAAATC CAAAGAACTG
    CCTTTCACAC CTTTGGGCTG GCATCATAAC AACCTGGAGC TGCTGAGGGA GGAGAATGGA
    GAGAAACAAG CTATGGAGAG GTTGCTTTCA GCTAATCATA ACCACATGAT GGCGCTGCTC
    CAACAGTTGC TCCATAGTGA GTCATTGTCA ACGTCTTGGA GGGACATCAT CGTATCCCTG
    GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGA ACCGGGATGA TGACATGGAT
    ATCCGCCAGT TTGTCCACAT CAAAAAAATC CCAGGTGGAA AGAAATTTGA TTCTGTGGTT
    GTCAATGGAT TTGTTTGCAC CAAGAATATT GCACATAAAA AGATGAATTC TTCTATTAAA
    AACCCCAAAA TTCTTCTGCT GAAATGTTCC ATTGAATATC TCTACAGAGA AGAAACCAAA
    TTTACTTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCCTAAAGAA TTATGTTCAG
    CGAATAGTTG ATGTTCGACC CACACTGGTT CTGGTTGAGA AAACGGTATC TCGAATTGCA
    CAGGATATGT TGCTGGAACA CGGCATTACT TTGGTCATTA ATGTAAAATC ACAAGTTTTG
    GAACGAGTTA GCCGGATGAC CCAAGGTGAT TTGGTGATGT CAATGGACCA GCTGCTTACC
    AAACCCCACC TGGGCACCTG TCACAAATTT TACATGCAGA TGTTTCAGTT GCCTAATGAA
    CAAACCAAGA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTGGGCTG TACAATCAAG
    CTTAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCTTAAT ATTTATGATC
    TGTGTAGCTT ATCATTCTCA ACTAGAAATC TCCTTCCTTA TGGATGAATT TGCTATGCCT
    CCTACATTAA CGCAAAACCC TTCCTTCCAT TCTCTGATTG AAGGACAGGA GGATGAAGGG
    GCTTCACAGG AGCCATTCAG CGGAAGTCCC CTCCCCCGGG AGCCTGACTT CCCCCCAGAC
    TTTCTGCCTT CTGATGATGG CAGTTTGCTA GAAACAAGGA TTCTGTTTGA GAAGGGTGAC
    CAGGAGAATA AGAGTGTGCC ACAGGATGTT GCCTCTTTGA AGCCTCAAGA GCATGCCCCA
    GCAGCTTGCC CGATGGGTGC CCCCTGTGCT CTCTTTCCAT CAGTACCAGA GTCATTGCTT
    CCGCTCCATG TGGATGACCA ACAGGACGCC ATAGGCAGTG AGCAGCCAGA GGCTTTGCAG
    CAAACAGAGG AGCTTCCGGA TCCCACAAGC CAGATGAGAG CCTTTAGAGA CCCTCTGCAG
    GATGACACTG GCCTGTATGT TACCGAGGAA GTTACCTCTT CTGAAGATAA ACGGAAGACT
    GATTCTTTGA CCTTTAAGCA AGAGTTGAAA GATGTGATCC TCTGTATCTC GCCAGTCATC
    ACATTCCGGG AGCCCTTCCT TTTAACTGAC AAGGGCATGA GATGCTCTAC CCGAGATTAT
    TTTGCGGAGC AGGTTTACTG GTCTCCTCTC CTCAATAAGG AGTTCAAGGA AATGGAGAGC
    AGGCGAAAGA AACAGATGCT TAGGGATCTC TCTGGCCTTC AGGGCATGAA TGGAAGTGTT
    CAGGCCAAGT CCATTCAAGT CCTGCCCTCA CATGAGCTCG TGAGCACCAG AATTGCTGAG
    CACCTGGGGG ACAGCCAGAG CTTGGGGAGA ATGCTAGCCG ATTATCGGGC CAGAGGAGGA
    AGAATCCAGC AAAAAAACTC AGACCCTTTT GCTTACTCAA AGGAGGCATC AAGCACCTCA
    AGTGGAAAAT CAGGAAGCAG AACTGAAGGT GATGAAGAAA AAGGGTTGAT TACCAGTGAT
    GCAGTGTGGT CGACAAAGGT GGACTGTCTG AACCCTGTCA ACCACCAGAG GCTGTGTGTG
    CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC AACGCTCCCA GTGCCTGTGT CAGTCCTTGG
    ATTGTAACAA TGGAATTTTA TGGAAAAAAT GATCTTACAT TAGGAATATT TTTAGAGAGA
    TACTGTTTCC GGCCTTCCTA TCAGTGCCCT AGCATGTTCT GTGATACCCC CATGGTGCAC
    CACATTCGAC GCTTTGTCCA TGGCCAAGGC TGTGTGCAGA TAATCCTAAA GGAGCTGGAT
    TCTCCAGTAC CTGGATATCA GCATACAATT CTCACCTACT CCTGGTGCAG AATGTGCAAA
    CAGGTAACAC CAGTTGTTGC TCTTTCCAAT GAGTCTTGGT CCATGTCGTT TGCAAAATAC
    CTGGAACTTA GGTTTTATGG GCACCAGTAC ACCCGCAGAG CCAATGCTGA GCCATGTGGT
    CACTCCATCC ACCACGATTA TCACCAGTAT TTCTCCTATA ACCAGATGGT GGCGTCTTTC
    AGTTATTCTC CCATTCGGCT TCTTGAAGTG TGTGTTCCAC TTCCCAAAAT ATTCATTAAA
    CGTCAAGTCC CACTAAAGGT GTCCCTTCTT CAGGATCTGA AGGACTTCTT TCAGAAAGTT
    TCACAGGTAT ATCTTGCTGT GGATGAAAGA CTTGCGTCTT TGAAAACTGA TACATTTAGC
    AAATCAAGAG AGGAAAAAAT GGAAGATATC TTTGCACAAA AAGAGATGGA AGAAAGTGAG
    TTCAAGAGCT GGACTGAGAA GATGCAGGCG CGGCTCCTGT CTTCCTCCAT GGATACCCCC
    CAGCAACTGC AGTCAGTCTT CGAGTCCCTC ATCGCCAAGA AACAAAGCCT CTGTGAGGTG
    CTGCAAGCTT GGAATAACAG GCTGCAAGAC CTTTTCCAAC AAGAAAAGGG TAGGAAGCGG
    CCTTCAGTTC CTCCAAGTCC TGGAAGACTG AGACAAGGTG AAGAAAGCAA GATAAGTGCA
    GTGGATGCAT CTCCACGGAA TGCTTCTCCA GGACTTCCAA ATGGAGAAAA AGAGGACCGC
    TTCTTAGCCA CCCTTTCCAG TCAGAGCTCC ACCAGTTCCC CCCATCTCCA GCTGCCTACT
    TCTCCTGAAG GCGTGCCTGA GCAGGTCATG GGAGGGCCCC CAGATCTCGA TACTGCCAGC
    AGTTCTGAAG ATGTATTTGA TGGACATTTG CTGGGATCTA CAGACAGCCA GGTGAAGGAA
    AAATCAACCA TGAAAGCCAT CTTTGCAAAT TTGCTTCCAG GAAATAGCTA TAACCCTATT
    CCATTTCCTT TCGATCCAGA TAAACACTAC TTAATGTATG AACATGAACG AGTGCCCATT
    GCAGTCTGTG AGAAGGAGCC CAGCTCCATC ATTGCTTTTG CTCTCAGTTG TAAAGAATAC
    CGGAATGCCT TAGAGGAATT GTCCAAAGCC ACTCAGCGGA ACAGTGCTGA AGAAGGGCTT
    CCAACAAACA GTACTTTAGA CAGCAGACCA AAGAGTAGCA GCCCAATTAG ACTACCTGAG
    ATCAGTGGAG GACAGACAAA CCGTGCGGTA GAAGCAGAAC CACAGCCAAC CAAAAAGGCT
    TCTGGAATGT TGTCCTTCTT CCGAGGGACA GCAGGGAAAA GCCCTGACCT CTCTTCCCAG
    AAGAGAGAGA CCTTACGTGG AGCAGATAGT GCTTACTACC AAGTGGGGCA GACGGGCAAG
    GAGGGGACGG AGAATCAAGG CATTGAGCCT CAAGATGAAG TAGATGGAGG AGATACACAG
    AAGAAACAAC TTACAAATCC TCATGTGGAG CTTCAATTTT CGGATGCCAA CGCCAAGTTT
    TACTGTCGGC TCTACTATGC GGGAGAGTTT CATAAGATGC GAGAAGTGAT TCTGGGCAGC
    AGCGAAGAAG ATTTCATTCG TTCCCTTTCT CACTCTTCTC CGTGGCAGGC CCGAGGAGGC
    AAATCAGGAG CTGCCTTTTA CGCTACTGAA GATGACAGAT TCATTTTGAA GCAAATGCCT
    CGTCTGGAAG TCCAGTCTTT CCTTGACTTC GCACCACACT ACTTCAATTA CATTACAAAT
    GCCGTTCAGC AAAAGAGGCC TACTGCATTG GCCAAGATTC TTGGAGTTTA CAGAATTGGT
    TATAAGAATT CTCAGAACAA CACTGAGAAG AAGCTAGATC TCCTTGTCAT GGAAAATCTT
    TTCTACGGGA GAAAGATGGC ACAGGTTTTT GACTTGAAGG GTTCACTTAG GAATCGAAAT
    GTAAAAACCG ATACCGGGAA AGAGAGCTGT GATGTTGTTC TTCTGGATGA AAATCTCTTA
    AAGATGGTTC GAGACAACCC TCTCTATATC CGTTCTCATT CCAAAGCTGT GCTGAGAGCC
    TCGATCCGGA GCGACTCCCA CTTCCTTTCT AGCCACCTCA TTATAGACTA TTCTTTGCTA
    GTTGGGCGAG ACGATACCAG CAATGAGCTA GTAGTTGGAA TTATAGATTA TATCCGAACA
    TTTACCTGGG ACAAAAAGCT TGAGATGGTT GTGAAATCAA CGGGAATTTT AGGAGGACAA
    GGTAAAATGC CGACTGTGGT CTCTCCAGAG TTGTACAGGA CTAGATTCTG TGAAGCCATG
    GACAAGTATT TCCTGATGGT GCCAGACCAC TGGACAGGCT TGGGTCTGAA CTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16660
    Clone ID Related Accession (Same CDS sequence) XM_005202762.4 , XM_005202762.3 , XM_024978630.1 , NM_001205467.1
    Accession Version XM_005202762.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6297bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202762.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGACACCAGA AGGAAGGCAG AACCTACTTT TGGAGGTCAT
    GACCCTCGCA CAGCTGTTCA ACTCCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA
    ATCATGGAGG GGAAGAGCCA GGATAGTGAC CTGAAGCAAT ACTGGATGCC AGATAGCCAG
    TGTAAAGAGT GCTATGACTG TAATGAGAAA TTTACCACTT TTAGGCGCAG ACACCACTGC
    CGACTCTGTG GGCAGATTTT CTGCAGTCGT TGCTGCAATC AAGAAATCCC TGGAAAATTT
    ATGGGTTATA CAGGGGACCT CCGAGCCTGT ACATACTGTA GAAAAATAGC CTTAAGTTAT
    GCTCATTCCA CAGACAGTAA TTCCATTGGT GAAGACTTGA ATGCTCTTTC AGACTCTGCT
    TCCTCTGTGT CTGTACTTGA TCCAAATGAA CCCCGTACAC CTGTGGGGAG TAGAAAAGCC
    AGCCGCAACA TATTTTTAGA GGATGATTTT GCCTGGCAAA GTTTAATTCA TCCAGACTCC
    TCAAACACTG CTCTTTCAAC AAGACTTGTA TCTGTTCAAG AGGATGCTGG GAAATCTCCT
    GCTCGAAATA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGATCTGC GTCTCCAATG
    GTACCATCAT ACGAGACGTC TGTCAGTCCC CAGGCTAACC GAACGTATGT TCGGACAGAG
    ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTTC AGTTAAAAGA CCTATGGAAG
    AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GCTGAGAACA
    CACCCCAACT GCATTGTAGG AAAGGAGTTA GTCAACTGGT TAATCCGAAA TGGACACATT
    GCTACAAGGG CACAGGCTAT AGCAATTGGA CAGGCAATGG TGGATGGACG TTGGCTGGAT
    TGTGTCAGTC ACCACGACCA GCTCTTCAGG GATGAGTATG CGCTGTACAG GCCGCTGCAG
    AGTACAGAAT TTTCCGAGAC CCCTTCTCCG GACAGTGACT CGGTGAACTC TGTGGAAGGA
    CACTCTGAGC CATCCTGGTT TAAAGACATC AAGTTTGATG ACAGTGACAC TGAGCAGATA
    GCTGAAGAAG GTGACGATAA TTTGACTAAC TCTGCCAGTC CTAGCAAGCG CACATCAGTC
    AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CCATCAGCCT GAACGTGGAG
    CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC
    CCTGCTGACC AAAAAGAGTA TTTGATTTCT GACAATGGAG GACAACAGCT ATCAATAAGT
    GACGCCTTCA TCAAAGAATC CTTATTTAAC CGTCGAGTTG AGGAAAAATC CAAAGAACTG
    CCTTTCACAC CTTTGGGCTG GCATCATAAC AACCTGGAGC TGCTGAGGGA GGAGAATGGA
    GAGAAACAAG CTATGGAGAG GTTGCTTTCA GCTAATCATA ACCACATGAT GGCGCTGCTC
    CAACAGTTGC TCCATAGTGA GTCATTGTCA ACGTCTTGGA GGGACATCAT CGTATCCCTG
    GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGA ACCGGGATGA TGACATGGAT
    ATCCGCCAGT TTGTCCACAT CAAAAAAATC CCAGGTGGAA AGAAATTTGA TTCTGTGGTT
    GTCAATGGAT TTGTTTGCAC CAAGAATATT GCACATAAAA AGATGAATTC TTCTATTAAA
    AACCCCAAAA TTCTTCTGCT GAAATGTTCC ATTGAATATC TCTACAGAGA AGAAACCAAA
    TTTACTTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCCTAAAGAA TTATGTTCAG
    CGAATAGTTG ATGTTCGACC CACACTGGTT CTGGTTGAGA AAACGGTATC TCGAATTGCA
    CAGGATATGT TGCTGGAACA CGGCATTACT TTGGTCATTA ATGTAAAATC ACAAGTTTTG
    GAACGAGTTA GCCGGATGAC CCAAGGTGAT TTGGTGATGT CAATGGACCA GCTGCTTACC
    AAACCCCACC TGGGCACCTG TCACAAATTT TACATGCAGA TGTTTCAGTT GCCTAATGAA
    CAAACCAAGA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTGGGCTG TACAATCAAG
    CTTAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCTTAAT ATTTATGATC
    TGTGTAGCTT ATCATTCTCA ACTAGAAATC TCCTTCCTTA TGGATGAATT TGCTATGCCT
    CCTACATTAA CGCAAAACCC TTCCTTCCAT TCTCTGATTG AAGGACAGGA GGATGAAGGG
    GCTTCACAGG AGCCATTCAG CGGAAGTCCC CTCCCCCGGG AGCCTGACTT CCCCCCAGAC
    TTTCTGCCTT CTGATGATGG CAGTTTGCTA GAAACAAGGA TTCTGTTTGA GAAGGGTGAC
    CAGGAGAATA AGAGTGTGCC ACAGGATGTT GCCTCTTTGA AGCCTCAAGA GCATGCCCCA
    GCAGCTTGCC CGATGGGTGC CCCCTGTGCT CTCTTTCCAT CAGTACCAGA GTCATTGCTT
    CCGCTCCATG TGGATGACCA ACAGGACGCC ATAGGCAGTG AGCAGCCAGA GGCTTTGCAG
    CAAACAGAGG AGCTTCCGGA TCCCACAAGC CAGATGAGAG CCTTTAGAGA CCCTCTGCAG
    GATGACACTG GCCTGTATGT TACCGAGGAA GTTACCTCTT CTGAAGATAA ACGGAAGACT
    GATTCTTTGA CCTTTAAGCA AGAGTTGAAA GATGTGATCC TCTGTATCTC GCCAGTCATC
    ACATTCCGGG AGCCCTTCCT TTTAACTGAC AAGGGCATGA GATGCTCTAC CCGAGATTAT
    TTTGCGGAGC AGGTTTACTG GTCTCCTCTC CTCAATAAGG AGTTCAAGGA AATGGAGAGC
    AGGCGAAAGA AACAGATGCT TAGGGATCTC TCTGGCCTTC AGGGCATGAA TGGAAGTGTT
    CAGGCCAAGT CCATTCAAGT CCTGCCCTCA CATGAGCTCG TGAGCACCAG AATTGCTGAG
    CACCTGGGGG ACAGCCAGAG CTTGGGGAGA ATGCTAGCCG ATTATCGGGC CAGAGGAGGA
    AGAATCCAGC AAAAAAACTC AGACCCTTTT GCTTACTCAA AGGAGGCATC AAGCACCTCA
    AGTGGAAAAT CAGGAAGCAG AACTGAAGGT GATGAAGAAA AAGGGTTGAT TACCAGTGAT
    GCAGTGTGGT CGACAAAGGT GGACTGTCTG AACCCTGTCA ACCACCAGAG GCTGTGTGTG
    CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC AACGCTCCCA GTGCCTGTGT CAGTCCTTGG
    ATTGTAACAA TGGAATTTTA TGGAAAAAAT GATCTTACAT TAGGAATATT TTTAGAGAGA
    TACTGTTTCC GGCCTTCCTA TCAGTGCCCT AGCATGTTCT GTGATACCCC CATGGTGCAC
    CACATTCGAC GCTTTGTCCA TGGCCAAGGC TGTGTGCAGA TAATCCTAAA GGAGCTGGAT
    TCTCCAGTAC CTGGATATCA GCATACAATT CTCACCTACT CCTGGTGCAG AATGTGCAAA
    CAGGTAACAC CAGTTGTTGC TCTTTCCAAT GAGTCTTGGT CCATGTCGTT TGCAAAATAC
    CTGGAACTTA GGTTTTATGG GCACCAGTAC ACCCGCAGAG CCAATGCTGA GCCATGTGGT
    CACTCCATCC ACCACGATTA TCACCAGTAT TTCTCCTATA ACCAGATGGT GGCGTCTTTC
    AGTTATTCTC CCATTCGGCT TCTTGAAGTG TGTGTTCCAC TTCCCAAAAT ATTCATTAAA
    CGTCAAGTCC CACTAAAGGT GTCCCTTCTT CAGGATCTGA AGGACTTCTT TCAGAAAGTT
    TCACAGGTAT ATCTTGCTGT GGATGAAAGA CTTGCGTCTT TGAAAACTGA TACATTTAGC
    AAATCAAGAG AGGAAAAAAT GGAAGATATC TTTGCACAAA AAGAGATGGA AGAAAGTGAG
    TTCAAGAGCT GGACTGAGAA GATGCAGGCG CGGCTCCTGT CTTCCTCCAT GGATACCCCC
    CAGCAACTGC AGTCAGTCTT CGAGTCCCTC ATCGCCAAGA AACAAAGCCT CTGTGAGGTG
    CTGCAAGCTT GGAATAACAG GCTGCAAGAC CTTTTCCAAC AAGAAAAGGG TAGGAAGCGG
    CCTTCAGTTC CTCCAAGTCC TGGAAGACTG AGACAAGGTG AAGAAAGCAA GATAAGTGCA
    GTGGATGCAT CTCCACGGAA TGCTTCTCCA GGACTTCCAA ATGGAGAAAA AGAGGACCGC
    TTCTTAGCCA CCCTTTCCAG TCAGAGCTCC ACCAGTTCCC CCCATCTCCA GCTGCCTACT
    TCTCCTGAAG GCGTGCCTGA GCAGGTCATG GGAGGGCCCC CAGATCTCGA TACTGCCAGC
    AGTTCTGAAG ATGTATTTGA TGGACATTTG CTGGGATCTA CAGACAGCCA GGTGAAGGAA
    AAATCAACCA TGAAAGCCAT CTTTGCAAAT TTGCTTCCAG GAAATAGCTA TAACCCTATT
    CCATTTCCTT TCGATCCAGA TAAACACTAC TTAATGTATG AACATGAACG AGTGCCCATT
    GCAGTCTGTG AGAAGGAGCC CAGCTCCATC ATTGCTTTTG CTCTCAGTTG TAAAGAATAC
    CGGAATGCCT TAGAGGAATT GTCCAAAGCC ACTCAGCGGA ACAGTGCTGA AGAAGGGCTT
    CCAACAAACA GTACTTTAGA CAGCAGACCA AAGAGTAGCA GCCCAATTAG ACTACCTGAG
    ATCAGTGGAG GACAGACAAA CCGTGCGGTA GAAGCAGAAC CACAGCCAAC CAAAAAGGCT
    TCTGGAATGT TGTCCTTCTT CCGAGGGACA GCAGGGAAAA GCCCTGACCT CTCTTCCCAG
    AAGAGAGAGA CCTTACGTGG AGCAGATAGT GCTTACTACC AAGTGGGGCA GACGGGCAAG
    GAGGGGACGG AGAATCAAGG CATTGAGCCT CAAGATGAAG TAGATGGAGG AGATACACAG
    AAGAAACAAC TTACAAATCC TCATGTGGAG CTTCAATTTT CGGATGCCAA CGCCAAGTTT
    TACTGTCGGC TCTACTATGC GGGAGAGTTT CATAAGATGC GAGAAGTGAT TCTGGGCAGC
    AGCGAAGAAG ATTTCATTCG TTCCCTTTCT CACTCTTCTC CGTGGCAGGC CCGAGGAGGC
    AAATCAGGAG CTGCCTTTTA CGCTACTGAA GATGACAGAT TCATTTTGAA GCAAATGCCT
    CGTCTGGAAG TCCAGTCTTT CCTTGACTTC GCACCACACT ACTTCAATTA CATTACAAAT
    GCCGTTCAGC AAAAGAGGCC TACTGCATTG GCCAAGATTC TTGGAGTTTA CAGAATTGGT
    TATAAGAATT CTCAGAACAA CACTGAGAAG AAGCTAGATC TCCTTGTCAT GGAAAATCTT
    TTCTACGGGA GAAAGATGGC ACAGGTTTTT GACTTGAAGG GTTCACTTAG GAATCGAAAT
    GTAAAAACCG ATACCGGGAA AGAGAGCTGT GATGTTGTTC TTCTGGATGA AAATCTCTTA
    AAGATGGTTC GAGACAACCC TCTCTATATC CGTTCTCATT CCAAAGCTGT GCTGAGAGCC
    TCGATCCGGA GCGACTCCCA CTTCCTTTCT AGCCACCTCA TTATAGACTA TTCTTTGCTA
    GTTGGGCGAG ACGATACCAG CAATGAGCTA GTAGTTGGAA TTATAGATTA TATCCGAACA
    TTTACCTGGG ACAAAAAGCT TGAGATGGTT GTGAAATCAA CGGGAATTTT AGGAGGACAA
    GGTAAAATGC CGACTGTGGT CTCTCCAGAG TTGTACAGGA CTAGATTCTG TGAAGCCATG
    GACAAGTATT TCCTGATGGT GCCAGACCAC TGGACAGGCT TGGGTCTGAA CTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202819.1
    CDS149..6445
    Translation

    Target ORF information:

    RefSeq Version XM_005202762.4
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202762.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGACACCAGA AGGAAGGCAG AACCTACTTT TGGAGGTCAT
    GACCCTCGCA CAGCTGTTCA ACTCCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA
    ATCATGGAGG GGAAGAGCCA GGATAGTGAC CTGAAGCAAT ACTGGATGCC AGATAGCCAG
    TGTAAAGAGT GCTATGACTG TAATGAGAAA TTTACCACTT TTAGGCGCAG ACACCACTGC
    CGACTCTGTG GGCAGATTTT CTGCAGTCGT TGCTGCAATC AAGAAATCCC TGGAAAATTT
    ATGGGTTATA CAGGGGACCT CCGAGCCTGT ACATACTGTA GAAAAATAGC CTTAAGTTAT
    GCTCATTCCA CAGACAGTAA TTCCATTGGT GAAGACTTGA ATGCTCTTTC AGACTCTGCT
    TCCTCTGTGT CTGTACTTGA TCCAAATGAA CCCCGTACAC CTGTGGGGAG TAGAAAAGCC
    AGCCGCAACA TATTTTTAGA GGATGATTTT GCCTGGCAAA GTTTAATTCA TCCAGACTCC
    TCAAACACTG CTCTTTCAAC AAGACTTGTA TCTGTTCAAG AGGATGCTGG GAAATCTCCT
    GCTCGAAATA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGATCTGC GTCTCCAATG
    GTACCATCAT ACGAGACGTC TGTCAGTCCC CAGGCTAACC GAACGTATGT TCGGACAGAG
    ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTTC AGTTAAAAGA CCTATGGAAG
    AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GCTGAGAACA
    CACCCCAACT GCATTGTAGG AAAGGAGTTA GTCAACTGGT TAATCCGAAA TGGACACATT
    GCTACAAGGG CACAGGCTAT AGCAATTGGA CAGGCAATGG TGGATGGACG TTGGCTGGAT
    TGTGTCAGTC ACCACGACCA GCTCTTCAGG GATGAGTATG CGCTGTACAG GCCGCTGCAG
    AGTACAGAAT TTTCCGAGAC CCCTTCTCCG GACAGTGACT CGGTGAACTC TGTGGAAGGA
    CACTCTGAGC CATCCTGGTT TAAAGACATC AAGTTTGATG ACAGTGACAC TGAGCAGATA
    GCTGAAGAAG GTGACGATAA TTTGACTAAC TCTGCCAGTC CTAGCAAGCG CACATCAGTC
    AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CCATCAGCCT GAACGTGGAG
    CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC
    CCTGCTGACC AAAAAGAGTA TTTGATTTCT GACAATGGAG GACAACAGCT ATCAATAAGT
    GACGCCTTCA TCAAAGAATC CTTATTTAAC CGTCGAGTTG AGGAAAAATC CAAAGAACTG
    CCTTTCACAC CTTTGGGCTG GCATCATAAC AACCTGGAGC TGCTGAGGGA GGAGAATGGA
    GAGAAACAAG CTATGGAGAG GTTGCTTTCA GCTAATCATA ACCACATGAT GGCGCTGCTC
    CAACAGTTGC TCCATAGTGA GTCATTGTCA ACGTCTTGGA GGGACATCAT CGTATCCCTG
    GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGA ACCGGGATGA TGACATGGAT
    ATCCGCCAGT TTGTCCACAT CAAAAAAATC CCAGGTGGAA AGAAATTTGA TTCTGTGGTT
    GTCAATGGAT TTGTTTGCAC CAAGAATATT GCACATAAAA AGATGAATTC TTCTATTAAA
    AACCCCAAAA TTCTTCTGCT GAAATGTTCC ATTGAATATC TCTACAGAGA AGAAACCAAA
    TTTACTTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCCTAAAGAA TTATGTTCAG
    CGAATAGTTG ATGTTCGACC CACACTGGTT CTGGTTGAGA AAACGGTATC TCGAATTGCA
    CAGGATATGT TGCTGGAACA CGGCATTACT TTGGTCATTA ATGTAAAATC ACAAGTTTTG
    GAACGAGTTA GCCGGATGAC CCAAGGTGAT TTGGTGATGT CAATGGACCA GCTGCTTACC
    AAACCCCACC TGGGCACCTG TCACAAATTT TACATGCAGA TGTTTCAGTT GCCTAATGAA
    CAAACCAAGA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTGGGCTG TACAATCAAG
    CTTAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCTTAAT ATTTATGATC
    TGTGTAGCTT ATCATTCTCA ACTAGAAATC TCCTTCCTTA TGGATGAATT TGCTATGCCT
    CCTACATTAA CGCAAAACCC TTCCTTCCAT TCTCTGATTG AAGGACAGGA GGATGAAGGG
    GCTTCACAGG AGCCATTCAG CGGAAGTCCC CTCCCCCGGG AGCCTGACTT CCCCCCAGAC
    TTTCTGCCTT CTGATGATGG CAGTTTGCTA GAAACAAGGA TTCTGTTTGA GAAGGGTGAC
    CAGGAGAATA AGAGTGTGCC ACAGGATGTT GCCTCTTTGA AGCCTCAAGA GCATGCCCCA
    GCAGCTTGCC CGATGGGTGC CCCCTGTGCT CTCTTTCCAT CAGTACCAGA GTCATTGCTT
    CCGCTCCATG TGGATGACCA ACAGGACGCC ATAGGCAGTG AGCAGCCAGA GGCTTTGCAG
    CAAACAGAGG AGCTTCCGGA TCCCACAAGC CAGATGAGAG CCTTTAGAGA CCCTCTGCAG
    GATGACACTG GCCTGTATGT TACCGAGGAA GTTACCTCTT CTGAAGATAA ACGGAAGACT
    GATTCTTTGA CCTTTAAGCA AGAGTTGAAA GATGTGATCC TCTGTATCTC GCCAGTCATC
    ACATTCCGGG AGCCCTTCCT TTTAACTGAC AAGGGCATGA GATGCTCTAC CCGAGATTAT
    TTTGCGGAGC AGGTTTACTG GTCTCCTCTC CTCAATAAGG AGTTCAAGGA AATGGAGAGC
    AGGCGAAAGA AACAGATGCT TAGGGATCTC TCTGGCCTTC AGGGCATGAA TGGAAGTGTT
    CAGGCCAAGT CCATTCAAGT CCTGCCCTCA CATGAGCTCG TGAGCACCAG AATTGCTGAG
    CACCTGGGGG ACAGCCAGAG CTTGGGGAGA ATGCTAGCCG ATTATCGGGC CAGAGGAGGA
    AGAATCCAGC AAAAAAACTC AGACCCTTTT GCTTACTCAA AGGAGGCATC AAGCACCTCA
    AGTGGAAAAT CAGGAAGCAG AACTGAAGGT GATGAAGAAA AAGGGTTGAT TACCAGTGAT
    GCAGTGTGGT CGACAAAGGT GGACTGTCTG AACCCTGTCA ACCACCAGAG GCTGTGTGTG
    CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC AACGCTCCCA GTGCCTGTGT CAGTCCTTGG
    ATTGTAACAA TGGAATTTTA TGGAAAAAAT GATCTTACAT TAGGAATATT TTTAGAGAGA
    TACTGTTTCC GGCCTTCCTA TCAGTGCCCT AGCATGTTCT GTGATACCCC CATGGTGCAC
    CACATTCGAC GCTTTGTCCA TGGCCAAGGC TGTGTGCAGA TAATCCTAAA GGAGCTGGAT
    TCTCCAGTAC CTGGATATCA GCATACAATT CTCACCTACT CCTGGTGCAG AATGTGCAAA
    CAGGTAACAC CAGTTGTTGC TCTTTCCAAT GAGTCTTGGT CCATGTCGTT TGCAAAATAC
    CTGGAACTTA GGTTTTATGG GCACCAGTAC ACCCGCAGAG CCAATGCTGA GCCATGTGGT
    CACTCCATCC ACCACGATTA TCACCAGTAT TTCTCCTATA ACCAGATGGT GGCGTCTTTC
    AGTTATTCTC CCATTCGGCT TCTTGAAGTG TGTGTTCCAC TTCCCAAAAT ATTCATTAAA
    CGTCAAGTCC CACTAAAGGT GTCCCTTCTT CAGGATCTGA AGGACTTCTT TCAGAAAGTT
    TCACAGGTAT ATCTTGCTGT GGATGAAAGA CTTGCGTCTT TGAAAACTGA TACATTTAGC
    AAATCAAGAG AGGAAAAAAT GGAAGATATC TTTGCACAAA AAGAGATGGA AGAAAGTGAG
    TTCAAGAGCT GGACTGAGAA GATGCAGGCG CGGCTCCTGT CTTCCTCCAT GGATACCCCC
    CAGCAACTGC AGTCAGTCTT CGAGTCCCTC ATCGCCAAGA AACAAAGCCT CTGTGAGGTG
    CTGCAAGCTT GGAATAACAG GCTGCAAGAC CTTTTCCAAC AAGAAAAGGG TAGGAAGCGG
    CCTTCAGTTC CTCCAAGTCC TGGAAGACTG AGACAAGGTG AAGAAAGCAA GATAAGTGCA
    GTGGATGCAT CTCCACGGAA TGCTTCTCCA GGACTTCCAA ATGGAGAAAA AGAGGACCGC
    TTCTTAGCCA CCCTTTCCAG TCAGAGCTCC ACCAGTTCCC CCCATCTCCA GCTGCCTACT
    TCTCCTGAAG GCGTGCCTGA GCAGGTCATG GGAGGGCCCC CAGATCTCGA TACTGCCAGC
    AGTTCTGAAG ATGTATTTGA TGGACATTTG CTGGGATCTA CAGACAGCCA GGTGAAGGAA
    AAATCAACCA TGAAAGCCAT CTTTGCAAAT TTGCTTCCAG GAAATAGCTA TAACCCTATT
    CCATTTCCTT TCGATCCAGA TAAACACTAC TTAATGTATG AACATGAACG AGTGCCCATT
    GCAGTCTGTG AGAAGGAGCC CAGCTCCATC ATTGCTTTTG CTCTCAGTTG TAAAGAATAC
    CGGAATGCCT TAGAGGAATT GTCCAAAGCC ACTCAGCGGA ACAGTGCTGA AGAAGGGCTT
    CCAACAAACA GTACTTTAGA CAGCAGACCA AAGAGTAGCA GCCCAATTAG ACTACCTGAG
    ATCAGTGGAG GACAGACAAA CCGTGCGGTA GAAGCAGAAC CACAGCCAAC CAAAAAGGCT
    TCTGGAATGT TGTCCTTCTT CCGAGGGACA GCAGGGAAAA GCCCTGACCT CTCTTCCCAG
    AAGAGAGAGA CCTTACGTGG AGCAGATAGT GCTTACTACC AAGTGGGGCA GACGGGCAAG
    GAGGGGACGG AGAATCAAGG CATTGAGCCT CAAGATGAAG TAGATGGAGG AGATACACAG
    AAGAAACAAC TTACAAATCC TCATGTGGAG CTTCAATTTT CGGATGCCAA CGCCAAGTTT
    TACTGTCGGC TCTACTATGC GGGAGAGTTT CATAAGATGC GAGAAGTGAT TCTGGGCAGC
    AGCGAAGAAG ATTTCATTCG TTCCCTTTCT CACTCTTCTC CGTGGCAGGC CCGAGGAGGC
    AAATCAGGAG CTGCCTTTTA CGCTACTGAA GATGACAGAT TCATTTTGAA GCAAATGCCT
    CGTCTGGAAG TCCAGTCTTT CCTTGACTTC GCACCACACT ACTTCAATTA CATTACAAAT
    GCCGTTCAGC AAAAGAGGCC TACTGCATTG GCCAAGATTC TTGGAGTTTA CAGAATTGGT
    TATAAGAATT CTCAGAACAA CACTGAGAAG AAGCTAGATC TCCTTGTCAT GGAAAATCTT
    TTCTACGGGA GAAAGATGGC ACAGGTTTTT GACTTGAAGG GTTCACTTAG GAATCGAAAT
    GTAAAAACCG ATACCGGGAA AGAGAGCTGT GATGTTGTTC TTCTGGATGA AAATCTCTTA
    AAGATGGTTC GAGACAACCC TCTCTATATC CGTTCTCATT CCAAAGCTGT GCTGAGAGCC
    TCGATCCGGA GCGACTCCCA CTTCCTTTCT AGCCACCTCA TTATAGACTA TTCTTTGCTA
    GTTGGGCGAG ACGATACCAG CAATGAGCTA GTAGTTGGAA TTATAGATTA TATCCGAACA
    TTTACCTGGG ACAAAAAGCT TGAGATGGTT GTGAAATCAA CGGGAATTTT AGGAGGACAA
    GGTAAAATGC CGACTGTGGT CTCTCCAGAG TTGTACAGGA CTAGATTCTG TGAAGCCATG
    GACAAGTATT TCCTGATGGT GCCAGACCAC TGGACAGGCT TGGGTCTGAA CTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16660
    Clone ID Related Accession (Same CDS sequence) XM_005202762.4 , XM_005202762.3 , XM_024978630.1 , NM_001205467.1
    Accession Version XM_005202762.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6297bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103846.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202762.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGACACCAGA AGGAAGGCAG AACCTACTTT TGGAGGTCAT
    GACCCTCGCA CAGCTGTTCA ACTCCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA
    ATCATGGAGG GGAAGAGCCA GGATAGTGAC CTGAAGCAAT ACTGGATGCC AGATAGCCAG
    TGTAAAGAGT GCTATGACTG TAATGAGAAA TTTACCACTT TTAGGCGCAG ACACCACTGC
    CGACTCTGTG GGCAGATTTT CTGCAGTCGT TGCTGCAATC AAGAAATCCC TGGAAAATTT
    ATGGGTTATA CAGGGGACCT CCGAGCCTGT ACATACTGTA GAAAAATAGC CTTAAGTTAT
    GCTCATTCCA CAGACAGTAA TTCCATTGGT GAAGACTTGA ATGCTCTTTC AGACTCTGCT
    TCCTCTGTGT CTGTACTTGA TCCAAATGAA CCCCGTACAC CTGTGGGGAG TAGAAAAGCC
    AGCCGCAACA TATTTTTAGA GGATGATTTT GCCTGGCAAA GTTTAATTCA TCCAGACTCC
    TCAAACACTG CTCTTTCAAC AAGACTTGTA TCTGTTCAAG AGGATGCTGG GAAATCTCCT
    GCTCGAAATA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGATCTGC GTCTCCAATG
    GTACCATCAT ACGAGACGTC TGTCAGTCCC CAGGCTAACC GAACGTATGT TCGGACAGAG
    ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTTC AGTTAAAAGA CCTATGGAAG
    AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GCTGAGAACA
    CACCCCAACT GCATTGTAGG AAAGGAGTTA GTCAACTGGT TAATCCGAAA TGGACACATT
    GCTACAAGGG CACAGGCTAT AGCAATTGGA CAGGCAATGG TGGATGGACG TTGGCTGGAT
    TGTGTCAGTC ACCACGACCA GCTCTTCAGG GATGAGTATG CGCTGTACAG GCCGCTGCAG
    AGTACAGAAT TTTCCGAGAC CCCTTCTCCG GACAGTGACT CGGTGAACTC TGTGGAAGGA
    CACTCTGAGC CATCCTGGTT TAAAGACATC AAGTTTGATG ACAGTGACAC TGAGCAGATA
    GCTGAAGAAG GTGACGATAA TTTGACTAAC TCTGCCAGTC CTAGCAAGCG CACATCAGTC
    AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CCATCAGCCT GAACGTGGAG
    CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC
    CCTGCTGACC AAAAAGAGTA TTTGATTTCT GACAATGGAG GACAACAGCT ATCAATAAGT
    GACGCCTTCA TCAAAGAATC CTTATTTAAC CGTCGAGTTG AGGAAAAATC CAAAGAACTG
    CCTTTCACAC CTTTGGGCTG GCATCATAAC AACCTGGAGC TGCTGAGGGA GGAGAATGGA
    GAGAAACAAG CTATGGAGAG GTTGCTTTCA GCTAATCATA ACCACATGAT GGCGCTGCTC
    CAACAGTTGC TCCATAGTGA GTCATTGTCA ACGTCTTGGA GGGACATCAT CGTATCCCTG
    GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGA ACCGGGATGA TGACATGGAT
    ATCCGCCAGT TTGTCCACAT CAAAAAAATC CCAGGTGGAA AGAAATTTGA TTCTGTGGTT
    GTCAATGGAT TTGTTTGCAC CAAGAATATT GCACATAAAA AGATGAATTC TTCTATTAAA
    AACCCCAAAA TTCTTCTGCT GAAATGTTCC ATTGAATATC TCTACAGAGA AGAAACCAAA
    TTTACTTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCCTAAAGAA TTATGTTCAG
    CGAATAGTTG ATGTTCGACC CACACTGGTT CTGGTTGAGA AAACGGTATC TCGAATTGCA
    CAGGATATGT TGCTGGAACA CGGCATTACT TTGGTCATTA ATGTAAAATC ACAAGTTTTG
    GAACGAGTTA GCCGGATGAC CCAAGGTGAT TTGGTGATGT CAATGGACCA GCTGCTTACC
    AAACCCCACC TGGGCACCTG TCACAAATTT TACATGCAGA TGTTTCAGTT GCCTAATGAA
    CAAACCAAGA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTGGGCTG TACAATCAAG
    CTTAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCTTAAT ATTTATGATC
    TGTGTAGCTT ATCATTCTCA ACTAGAAATC TCCTTCCTTA TGGATGAATT TGCTATGCCT
    CCTACATTAA CGCAAAACCC TTCCTTCCAT TCTCTGATTG AAGGACAGGA GGATGAAGGG
    GCTTCACAGG AGCCATTCAG CGGAAGTCCC CTCCCCCGGG AGCCTGACTT CCCCCCAGAC
    TTTCTGCCTT CTGATGATGG CAGTTTGCTA GAAACAAGGA TTCTGTTTGA GAAGGGTGAC
    CAGGAGAATA AGAGTGTGCC ACAGGATGTT GCCTCTTTGA AGCCTCAAGA GCATGCCCCA
    GCAGCTTGCC CGATGGGTGC CCCCTGTGCT CTCTTTCCAT CAGTACCAGA GTCATTGCTT
    CCGCTCCATG TGGATGACCA ACAGGACGCC ATAGGCAGTG AGCAGCCAGA GGCTTTGCAG
    CAAACAGAGG AGCTTCCGGA TCCCACAAGC CAGATGAGAG CCTTTAGAGA CCCTCTGCAG
    GATGACACTG GCCTGTATGT TACCGAGGAA GTTACCTCTT CTGAAGATAA ACGGAAGACT
    GATTCTTTGA CCTTTAAGCA AGAGTTGAAA GATGTGATCC TCTGTATCTC GCCAGTCATC
    ACATTCCGGG AGCCCTTCCT TTTAACTGAC AAGGGCATGA GATGCTCTAC CCGAGATTAT
    TTTGCGGAGC AGGTTTACTG GTCTCCTCTC CTCAATAAGG AGTTCAAGGA AATGGAGAGC
    AGGCGAAAGA AACAGATGCT TAGGGATCTC TCTGGCCTTC AGGGCATGAA TGGAAGTGTT
    CAGGCCAAGT CCATTCAAGT CCTGCCCTCA CATGAGCTCG TGAGCACCAG AATTGCTGAG
    CACCTGGGGG ACAGCCAGAG CTTGGGGAGA ATGCTAGCCG ATTATCGGGC CAGAGGAGGA
    AGAATCCAGC AAAAAAACTC AGACCCTTTT GCTTACTCAA AGGAGGCATC AAGCACCTCA
    AGTGGAAAAT CAGGAAGCAG AACTGAAGGT GATGAAGAAA AAGGGTTGAT TACCAGTGAT
    GCAGTGTGGT CGACAAAGGT GGACTGTCTG AACCCTGTCA ACCACCAGAG GCTGTGTGTG
    CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC AACGCTCCCA GTGCCTGTGT CAGTCCTTGG
    ATTGTAACAA TGGAATTTTA TGGAAAAAAT GATCTTACAT TAGGAATATT TTTAGAGAGA
    TACTGTTTCC GGCCTTCCTA TCAGTGCCCT AGCATGTTCT GTGATACCCC CATGGTGCAC
    CACATTCGAC GCTTTGTCCA TGGCCAAGGC TGTGTGCAGA TAATCCTAAA GGAGCTGGAT
    TCTCCAGTAC CTGGATATCA GCATACAATT CTCACCTACT CCTGGTGCAG AATGTGCAAA
    CAGGTAACAC CAGTTGTTGC TCTTTCCAAT GAGTCTTGGT CCATGTCGTT TGCAAAATAC
    CTGGAACTTA GGTTTTATGG GCACCAGTAC ACCCGCAGAG CCAATGCTGA GCCATGTGGT
    CACTCCATCC ACCACGATTA TCACCAGTAT TTCTCCTATA ACCAGATGGT GGCGTCTTTC
    AGTTATTCTC CCATTCGGCT TCTTGAAGTG TGTGTTCCAC TTCCCAAAAT ATTCATTAAA
    CGTCAAGTCC CACTAAAGGT GTCCCTTCTT CAGGATCTGA AGGACTTCTT TCAGAAAGTT
    TCACAGGTAT ATCTTGCTGT GGATGAAAGA CTTGCGTCTT TGAAAACTGA TACATTTAGC
    AAATCAAGAG AGGAAAAAAT GGAAGATATC TTTGCACAAA AAGAGATGGA AGAAAGTGAG
    TTCAAGAGCT GGACTGAGAA GATGCAGGCG CGGCTCCTGT CTTCCTCCAT GGATACCCCC
    CAGCAACTGC AGTCAGTCTT CGAGTCCCTC ATCGCCAAGA AACAAAGCCT CTGTGAGGTG
    CTGCAAGCTT GGAATAACAG GCTGCAAGAC CTTTTCCAAC AAGAAAAGGG TAGGAAGCGG
    CCTTCAGTTC CTCCAAGTCC TGGAAGACTG AGACAAGGTG AAGAAAGCAA GATAAGTGCA
    GTGGATGCAT CTCCACGGAA TGCTTCTCCA GGACTTCCAA ATGGAGAAAA AGAGGACCGC
    TTCTTAGCCA CCCTTTCCAG TCAGAGCTCC ACCAGTTCCC CCCATCTCCA GCTGCCTACT
    TCTCCTGAAG GCGTGCCTGA GCAGGTCATG GGAGGGCCCC CAGATCTCGA TACTGCCAGC
    AGTTCTGAAG ATGTATTTGA TGGACATTTG CTGGGATCTA CAGACAGCCA GGTGAAGGAA
    AAATCAACCA TGAAAGCCAT CTTTGCAAAT TTGCTTCCAG GAAATAGCTA TAACCCTATT
    CCATTTCCTT TCGATCCAGA TAAACACTAC TTAATGTATG AACATGAACG AGTGCCCATT
    GCAGTCTGTG AGAAGGAGCC CAGCTCCATC ATTGCTTTTG CTCTCAGTTG TAAAGAATAC
    CGGAATGCCT TAGAGGAATT GTCCAAAGCC ACTCAGCGGA ACAGTGCTGA AGAAGGGCTT
    CCAACAAACA GTACTTTAGA CAGCAGACCA AAGAGTAGCA GCCCAATTAG ACTACCTGAG
    ATCAGTGGAG GACAGACAAA CCGTGCGGTA GAAGCAGAAC CACAGCCAAC CAAAAAGGCT
    TCTGGAATGT TGTCCTTCTT CCGAGGGACA GCAGGGAAAA GCCCTGACCT CTCTTCCCAG
    AAGAGAGAGA CCTTACGTGG AGCAGATAGT GCTTACTACC AAGTGGGGCA GACGGGCAAG
    GAGGGGACGG AGAATCAAGG CATTGAGCCT CAAGATGAAG TAGATGGAGG AGATACACAG
    AAGAAACAAC TTACAAATCC TCATGTGGAG CTTCAATTTT CGGATGCCAA CGCCAAGTTT
    TACTGTCGGC TCTACTATGC GGGAGAGTTT CATAAGATGC GAGAAGTGAT TCTGGGCAGC
    AGCGAAGAAG ATTTCATTCG TTCCCTTTCT CACTCTTCTC CGTGGCAGGC CCGAGGAGGC
    AAATCAGGAG CTGCCTTTTA CGCTACTGAA GATGACAGAT TCATTTTGAA GCAAATGCCT
    CGTCTGGAAG TCCAGTCTTT CCTTGACTTC GCACCACACT ACTTCAATTA CATTACAAAT
    GCCGTTCAGC AAAAGAGGCC TACTGCATTG GCCAAGATTC TTGGAGTTTA CAGAATTGGT
    TATAAGAATT CTCAGAACAA CACTGAGAAG AAGCTAGATC TCCTTGTCAT GGAAAATCTT
    TTCTACGGGA GAAAGATGGC ACAGGTTTTT GACTTGAAGG GTTCACTTAG GAATCGAAAT
    GTAAAAACCG ATACCGGGAA AGAGAGCTGT GATGTTGTTC TTCTGGATGA AAATCTCTTA
    AAGATGGTTC GAGACAACCC TCTCTATATC CGTTCTCATT CCAAAGCTGT GCTGAGAGCC
    TCGATCCGGA GCGACTCCCA CTTCCTTTCT AGCCACCTCA TTATAGACTA TTCTTTGCTA
    GTTGGGCGAG ACGATACCAG CAATGAGCTA GTAGTTGGAA TTATAGATTA TATCCGAACA
    TTTACCTGGG ACAAAAAGCT TGAGATGGTT GTGAAATCAA CGGGAATTTT AGGAGGACAA
    GGTAAAATGC CGACTGTGGT CTCTCCAGAG TTGTACAGGA CTAGATTCTG TGAAGCCATG
    GACAAGTATT TCCTGATGGT GCCAGACCAC TGGACAGGCT TGGGTCTGAA CTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202819.1
    CDS132..6428
    Translation

    Target ORF information:

    RefSeq Version XM_005202762.3
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202762.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGACACCAGA AGGAAGGCAG AACCTACTTT TGGAGGTCAT
    GACCCTCGCA CAGCTGTTCA ACTCCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA
    ATCATGGAGG GGAAGAGCCA GGATAGTGAC CTGAAGCAAT ACTGGATGCC AGATAGCCAG
    TGTAAAGAGT GCTATGACTG TAATGAGAAA TTTACCACTT TTAGGCGCAG ACACCACTGC
    CGACTCTGTG GGCAGATTTT CTGCAGTCGT TGCTGCAATC AAGAAATCCC TGGAAAATTT
    ATGGGTTATA CAGGGGACCT CCGAGCCTGT ACATACTGTA GAAAAATAGC CTTAAGTTAT
    GCTCATTCCA CAGACAGTAA TTCCATTGGT GAAGACTTGA ATGCTCTTTC AGACTCTGCT
    TCCTCTGTGT CTGTACTTGA TCCAAATGAA CCCCGTACAC CTGTGGGGAG TAGAAAAGCC
    AGCCGCAACA TATTTTTAGA GGATGATTTT GCCTGGCAAA GTTTAATTCA TCCAGACTCC
    TCAAACACTG CTCTTTCAAC AAGACTTGTA TCTGTTCAAG AGGATGCTGG GAAATCTCCT
    GCTCGAAATA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGATCTGC GTCTCCAATG
    GTACCATCAT ACGAGACGTC TGTCAGTCCC CAGGCTAACC GAACGTATGT TCGGACAGAG
    ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTTC AGTTAAAAGA CCTATGGAAG
    AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GCTGAGAACA
    CACCCCAACT GCATTGTAGG AAAGGAGTTA GTCAACTGGT TAATCCGAAA TGGACACATT
    GCTACAAGGG CACAGGCTAT AGCAATTGGA CAGGCAATGG TGGATGGACG TTGGCTGGAT
    TGTGTCAGTC ACCACGACCA GCTCTTCAGG GATGAGTATG CGCTGTACAG GCCGCTGCAG
    AGTACAGAAT TTTCCGAGAC CCCTTCTCCG GACAGTGACT CGGTGAACTC TGTGGAAGGA
    CACTCTGAGC CATCCTGGTT TAAAGACATC AAGTTTGATG ACAGTGACAC TGAGCAGATA
    GCTGAAGAAG GTGACGATAA TTTGACTAAC TCTGCCAGTC CTAGCAAGCG CACATCAGTC
    AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CCATCAGCCT GAACGTGGAG
    CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC
    CCTGCTGACC AAAAAGAGTA TTTGATTTCT GACAATGGAG GACAACAGCT ATCAATAAGT
    GACGCCTTCA TCAAAGAATC CTTATTTAAC CGTCGAGTTG AGGAAAAATC CAAAGAACTG
    CCTTTCACAC CTTTGGGCTG GCATCATAAC AACCTGGAGC TGCTGAGGGA GGAGAATGGA
    GAGAAACAAG CTATGGAGAG GTTGCTTTCA GCTAATCATA ACCACATGAT GGCGCTGCTC
    CAACAGTTGC TCCATAGTGA GTCATTGTCA ACGTCTTGGA GGGACATCAT CGTATCCCTG
    GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGA ACCGGGATGA TGACATGGAT
    ATCCGCCAGT TTGTCCACAT CAAAAAAATC CCAGGTGGAA AGAAATTTGA TTCTGTGGTT
    GTCAATGGAT TTGTTTGCAC CAAGAATATT GCACATAAAA AGATGAATTC TTCTATTAAA
    AACCCCAAAA TTCTTCTGCT GAAATGTTCC ATTGAATATC TCTACAGAGA AGAAACCAAA
    TTTACTTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCCTAAAGAA TTATGTTCAG
    CGAATAGTTG ATGTTCGACC CACACTGGTT CTGGTTGAGA AAACGGTATC TCGAATTGCA
    CAGGATATGT TGCTGGAACA CGGCATTACT TTGGTCATTA ATGTAAAATC ACAAGTTTTG
    GAACGAGTTA GCCGGATGAC CCAAGGTGAT TTGGTGATGT CAATGGACCA GCTGCTTACC
    AAACCCCACC TGGGCACCTG TCACAAATTT TACATGCAGA TGTTTCAGTT GCCTAATGAA
    CAAACCAAGA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTGGGCTG TACAATCAAG
    CTTAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCTTAAT ATTTATGATC
    TGTGTAGCTT ATCATTCTCA ACTAGAAATC TCCTTCCTTA TGGATGAATT TGCTATGCCT
    CCTACATTAA CGCAAAACCC TTCCTTCCAT TCTCTGATTG AAGGACAGGA GGATGAAGGG
    GCTTCACAGG AGCCATTCAG CGGAAGTCCC CTCCCCCGGG AGCCTGACTT CCCCCCAGAC
    TTTCTGCCTT CTGATGATGG CAGTTTGCTA GAAACAAGGA TTCTGTTTGA GAAGGGTGAC
    CAGGAGAATA AGAGTGTGCC ACAGGATGTT GCCTCTTTGA AGCCTCAAGA GCATGCCCCA
    GCAGCTTGCC CGATGGGTGC CCCCTGTGCT CTCTTTCCAT CAGTACCAGA GTCATTGCTT
    CCGCTCCATG TGGATGACCA ACAGGACGCC ATAGGCAGTG AGCAGCCAGA GGCTTTGCAG
    CAAACAGAGG AGCTTCCGGA TCCCACAAGC CAGATGAGAG CCTTTAGAGA CCCTCTGCAG
    GATGACACTG GCCTGTATGT TACCGAGGAA GTTACCTCTT CTGAAGATAA ACGGAAGACT
    GATTCTTTGA CCTTTAAGCA AGAGTTGAAA GATGTGATCC TCTGTATCTC GCCAGTCATC
    ACATTCCGGG AGCCCTTCCT TTTAACTGAC AAGGGCATGA GATGCTCTAC CCGAGATTAT
    TTTGCGGAGC AGGTTTACTG GTCTCCTCTC CTCAATAAGG AGTTCAAGGA AATGGAGAGC
    AGGCGAAAGA AACAGATGCT TAGGGATCTC TCTGGCCTTC AGGGCATGAA TGGAAGTGTT
    CAGGCCAAGT CCATTCAAGT CCTGCCCTCA CATGAGCTCG TGAGCACCAG AATTGCTGAG
    CACCTGGGGG ACAGCCAGAG CTTGGGGAGA ATGCTAGCCG ATTATCGGGC CAGAGGAGGA
    AGAATCCAGC AAAAAAACTC AGACCCTTTT GCTTACTCAA AGGAGGCATC AAGCACCTCA
    AGTGGAAAAT CAGGAAGCAG AACTGAAGGT GATGAAGAAA AAGGGTTGAT TACCAGTGAT
    GCAGTGTGGT CGACAAAGGT GGACTGTCTG AACCCTGTCA ACCACCAGAG GCTGTGTGTG
    CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC AACGCTCCCA GTGCCTGTGT CAGTCCTTGG
    ATTGTAACAA TGGAATTTTA TGGAAAAAAT GATCTTACAT TAGGAATATT TTTAGAGAGA
    TACTGTTTCC GGCCTTCCTA TCAGTGCCCT AGCATGTTCT GTGATACCCC CATGGTGCAC
    CACATTCGAC GCTTTGTCCA TGGCCAAGGC TGTGTGCAGA TAATCCTAAA GGAGCTGGAT
    TCTCCAGTAC CTGGATATCA GCATACAATT CTCACCTACT CCTGGTGCAG AATGTGCAAA
    CAGGTAACAC CAGTTGTTGC TCTTTCCAAT GAGTCTTGGT CCATGTCGTT TGCAAAATAC
    CTGGAACTTA GGTTTTATGG GCACCAGTAC ACCCGCAGAG CCAATGCTGA GCCATGTGGT
    CACTCCATCC ACCACGATTA TCACCAGTAT TTCTCCTATA ACCAGATGGT GGCGTCTTTC
    AGTTATTCTC CCATTCGGCT TCTTGAAGTG TGTGTTCCAC TTCCCAAAAT ATTCATTAAA
    CGTCAAGTCC CACTAAAGGT GTCCCTTCTT CAGGATCTGA AGGACTTCTT TCAGAAAGTT
    TCACAGGTAT ATCTTGCTGT GGATGAAAGA CTTGCGTCTT TGAAAACTGA TACATTTAGC
    AAATCAAGAG AGGAAAAAAT GGAAGATATC TTTGCACAAA AAGAGATGGA AGAAAGTGAG
    TTCAAGAGCT GGACTGAGAA GATGCAGGCG CGGCTCCTGT CTTCCTCCAT GGATACCCCC
    CAGCAACTGC AGTCAGTCTT CGAGTCCCTC ATCGCCAAGA AACAAAGCCT CTGTGAGGTG
    CTGCAAGCTT GGAATAACAG GCTGCAAGAC CTTTTCCAAC AAGAAAAGGG TAGGAAGCGG
    CCTTCAGTTC CTCCAAGTCC TGGAAGACTG AGACAAGGTG AAGAAAGCAA GATAAGTGCA
    GTGGATGCAT CTCCACGGAA TGCTTCTCCA GGACTTCCAA ATGGAGAAAA AGAGGACCGC
    TTCTTAGCCA CCCTTTCCAG TCAGAGCTCC ACCAGTTCCC CCCATCTCCA GCTGCCTACT
    TCTCCTGAAG GCGTGCCTGA GCAGGTCATG GGAGGGCCCC CAGATCTCGA TACTGCCAGC
    AGTTCTGAAG ATGTATTTGA TGGACATTTG CTGGGATCTA CAGACAGCCA GGTGAAGGAA
    AAATCAACCA TGAAAGCCAT CTTTGCAAAT TTGCTTCCAG GAAATAGCTA TAACCCTATT
    CCATTTCCTT TCGATCCAGA TAAACACTAC TTAATGTATG AACATGAACG AGTGCCCATT
    GCAGTCTGTG AGAAGGAGCC CAGCTCCATC ATTGCTTTTG CTCTCAGTTG TAAAGAATAC
    CGGAATGCCT TAGAGGAATT GTCCAAAGCC ACTCAGCGGA ACAGTGCTGA AGAAGGGCTT
    CCAACAAACA GTACTTTAGA CAGCAGACCA AAGAGTAGCA GCCCAATTAG ACTACCTGAG
    ATCAGTGGAG GACAGACAAA CCGTGCGGTA GAAGCAGAAC CACAGCCAAC CAAAAAGGCT
    TCTGGAATGT TGTCCTTCTT CCGAGGGACA GCAGGGAAAA GCCCTGACCT CTCTTCCCAG
    AAGAGAGAGA CCTTACGTGG AGCAGATAGT GCTTACTACC AAGTGGGGCA GACGGGCAAG
    GAGGGGACGG AGAATCAAGG CATTGAGCCT CAAGATGAAG TAGATGGAGG AGATACACAG
    AAGAAACAAC TTACAAATCC TCATGTGGAG CTTCAATTTT CGGATGCCAA CGCCAAGTTT
    TACTGTCGGC TCTACTATGC GGGAGAGTTT CATAAGATGC GAGAAGTGAT TCTGGGCAGC
    AGCGAAGAAG ATTTCATTCG TTCCCTTTCT CACTCTTCTC CGTGGCAGGC CCGAGGAGGC
    AAATCAGGAG CTGCCTTTTA CGCTACTGAA GATGACAGAT TCATTTTGAA GCAAATGCCT
    CGTCTGGAAG TCCAGTCTTT CCTTGACTTC GCACCACACT ACTTCAATTA CATTACAAAT
    GCCGTTCAGC AAAAGAGGCC TACTGCATTG GCCAAGATTC TTGGAGTTTA CAGAATTGGT
    TATAAGAATT CTCAGAACAA CACTGAGAAG AAGCTAGATC TCCTTGTCAT GGAAAATCTT
    TTCTACGGGA GAAAGATGGC ACAGGTTTTT GACTTGAAGG GTTCACTTAG GAATCGAAAT
    GTAAAAACCG ATACCGGGAA AGAGAGCTGT GATGTTGTTC TTCTGGATGA AAATCTCTTA
    AAGATGGTTC GAGACAACCC TCTCTATATC CGTTCTCATT CCAAAGCTGT GCTGAGAGCC
    TCGATCCGGA GCGACTCCCA CTTCCTTTCT AGCCACCTCA TTATAGACTA TTCTTTGCTA
    GTTGGGCGAG ACGATACCAG CAATGAGCTA GTAGTTGGAA TTATAGATTA TATCCGAACA
    TTTACCTGGG ACAAAAAGCT TGAGATGGTT GTGAAATCAA CGGGAATTTT AGGAGGACAA
    GGTAAAATGC CGACTGTGGT CTCTCCAGAG TTGTACAGGA CTAGATTCTG TGAAGCCATG
    GACAAGTATT TCCTGATGGT GCCAGACCAC TGGACAGGCT TGGGTCTGAA CTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16660
    Clone ID Related Accession (Same CDS sequence) XM_005202762.4 , XM_005202762.3 , XM_024978630.1 , NM_001205467.1
    Accession Version XM_024978630.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6297bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGACACCAGA AGGAAGGCAG AACCTACTTT TGGAGGTCAT
    GACCCTCGCA CAGCTGTTCA ACTCCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA
    ATCATGGAGG GGAAGAGCCA GGATAGTGAC CTGAAGCAAT ACTGGATGCC AGATAGCCAG
    TGTAAAGAGT GCTATGACTG TAATGAGAAA TTTACCACTT TTAGGCGCAG ACACCACTGC
    CGACTCTGTG GGCAGATTTT CTGCAGTCGT TGCTGCAATC AAGAAATCCC TGGAAAATTT
    ATGGGTTATA CAGGGGACCT CCGAGCCTGT ACATACTGTA GAAAAATAGC CTTAAGTTAT
    GCTCATTCCA CAGACAGTAA TTCCATTGGT GAAGACTTGA ATGCTCTTTC AGACTCTGCT
    TCCTCTGTGT CTGTACTTGA TCCAAATGAA CCCCGTACAC CTGTGGGGAG TAGAAAAGCC
    AGCCGCAACA TATTTTTAGA GGATGATTTT GCCTGGCAAA GTTTAATTCA TCCAGACTCC
    TCAAACACTG CTCTTTCAAC AAGACTTGTA TCTGTTCAAG AGGATGCTGG GAAATCTCCT
    GCTCGAAATA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGATCTGC GTCTCCAATG
    GTACCATCAT ACGAGACGTC TGTCAGTCCC CAGGCTAACC GAACGTATGT TCGGACAGAG
    ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTTC AGTTAAAAGA CCTATGGAAG
    AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GCTGAGAACA
    CACCCCAACT GCATTGTAGG AAAGGAGTTA GTCAACTGGT TAATCCGAAA TGGACACATT
    GCTACAAGGG CACAGGCTAT AGCAATTGGA CAGGCAATGG TGGATGGACG TTGGCTGGAT
    TGTGTCAGTC ACCACGACCA GCTCTTCAGG GATGAGTATG CGCTGTACAG GCCGCTGCAG
    AGTACAGAAT TTTCCGAGAC CCCTTCTCCG GACAGTGACT CGGTGAACTC TGTGGAAGGA
    CACTCTGAGC CATCCTGGTT TAAAGACATC AAGTTTGATG ACAGTGACAC TGAGCAGATA
    GCTGAAGAAG GTGACGATAA TTTGACTAAC TCTGCCAGTC CTAGCAAGCG CACATCAGTC
    AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CCATCAGCCT GAACGTGGAG
    CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC
    CCTGCTGACC AAAAAGAGTA TTTGATTTCT GACAATGGAG GACAACAGCT ATCAATAAGT
    GACGCCTTCA TCAAAGAATC CTTATTTAAC CGTCGAGTTG AGGAAAAATC CAAAGAACTG
    CCTTTCACAC CTTTGGGCTG GCATCATAAC AACCTGGAGC TGCTGAGGGA GGAGAATGGA
    GAGAAACAAG CTATGGAGAG GTTGCTTTCA GCTAATCATA ACCACATGAT GGCGCTGCTC
    CAACAGTTGC TCCATAGTGA GTCATTGTCA ACGTCTTGGA GGGACATCAT CGTATCCCTG
    GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGA ACCGGGATGA TGACATGGAT
    ATCCGCCAGT TTGTCCACAT CAAAAAAATC CCAGGTGGAA AGAAATTTGA TTCTGTGGTT
    GTCAATGGAT TTGTTTGCAC CAAGAATATT GCACATAAAA AGATGAATTC TTCTATTAAA
    AACCCCAAAA TTCTTCTGCT GAAATGTTCC ATTGAATATC TCTACAGAGA AGAAACCAAA
    TTTACTTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCCTAAAGAA TTATGTTCAG
    CGAATAGTTG ATGTTCGACC CACACTGGTT CTGGTTGAGA AAACGGTATC TCGAATTGCA
    CAGGATATGT TGCTGGAACA CGGCATTACT TTGGTCATTA ATGTAAAATC ACAAGTTTTG
    GAACGAGTTA GCCGGATGAC CCAAGGTGAT TTGGTGATGT CAATGGACCA GCTGCTTACC
    AAACCCCACC TGGGCACCTG TCACAAATTT TACATGCAGA TGTTTCAGTT GCCTAATGAA
    CAAACCAAGA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTGGGCTG TACAATCAAG
    CTTAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCTTAAT ATTTATGATC
    TGTGTAGCTT ATCATTCTCA ACTAGAAATC TCCTTCCTTA TGGATGAATT TGCTATGCCT
    CCTACATTAA CGCAAAACCC TTCCTTCCAT TCTCTGATTG AAGGACAGGA GGATGAAGGG
    GCTTCACAGG AGCCATTCAG CGGAAGTCCC CTCCCCCGGG AGCCTGACTT CCCCCCAGAC
    TTTCTGCCTT CTGATGATGG CAGTTTGCTA GAAACAAGGA TTCTGTTTGA GAAGGGTGAC
    CAGGAGAATA AGAGTGTGCC ACAGGATGTT GCCTCTTTGA AGCCTCAAGA GCATGCCCCA
    GCAGCTTGCC CGATGGGTGC CCCCTGTGCT CTCTTTCCAT CAGTACCAGA GTCATTGCTT
    CCGCTCCATG TGGATGACCA ACAGGACGCC ATAGGCAGTG AGCAGCCAGA GGCTTTGCAG
    CAAACAGAGG AGCTTCCGGA TCCCACAAGC CAGATGAGAG CCTTTAGAGA CCCTCTGCAG
    GATGACACTG GCCTGTATGT TACCGAGGAA GTTACCTCTT CTGAAGATAA ACGGAAGACT
    GATTCTTTGA CCTTTAAGCA AGAGTTGAAA GATGTGATCC TCTGTATCTC GCCAGTCATC
    ACATTCCGGG AGCCCTTCCT TTTAACTGAC AAGGGCATGA GATGCTCTAC CCGAGATTAT
    TTTGCGGAGC AGGTTTACTG GTCTCCTCTC CTCAATAAGG AGTTCAAGGA AATGGAGAGC
    AGGCGAAAGA AACAGATGCT TAGGGATCTC TCTGGCCTTC AGGGCATGAA TGGAAGTGTT
    CAGGCCAAGT CCATTCAAGT CCTGCCCTCA CATGAGCTCG TGAGCACCAG AATTGCTGAG
    CACCTGGGGG ACAGCCAGAG CTTGGGGAGA ATGCTAGCCG ATTATCGGGC CAGAGGAGGA
    AGAATCCAGC AAAAAAACTC AGACCCTTTT GCTTACTCAA AGGAGGCATC AAGCACCTCA
    AGTGGAAAAT CAGGAAGCAG AACTGAAGGT GATGAAGAAA AAGGGTTGAT TACCAGTGAT
    GCAGTGTGGT CGACAAAGGT GGACTGTCTG AACCCTGTCA ACCACCAGAG GCTGTGTGTG
    CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC AACGCTCCCA GTGCCTGTGT CAGTCCTTGG
    ATTGTAACAA TGGAATTTTA TGGAAAAAAT GATCTTACAT TAGGAATATT TTTAGAGAGA
    TACTGTTTCC GGCCTTCCTA TCAGTGCCCT AGCATGTTCT GTGATACCCC CATGGTGCAC
    CACATTCGAC GCTTTGTCCA TGGCCAAGGC TGTGTGCAGA TAATCCTAAA GGAGCTGGAT
    TCTCCAGTAC CTGGATATCA GCATACAATT CTCACCTACT CCTGGTGCAG AATGTGCAAA
    CAGGTAACAC CAGTTGTTGC TCTTTCCAAT GAGTCTTGGT CCATGTCGTT TGCAAAATAC
    CTGGAACTTA GGTTTTATGG GCACCAGTAC ACCCGCAGAG CCAATGCTGA GCCATGTGGT
    CACTCCATCC ACCACGATTA TCACCAGTAT TTCTCCTATA ACCAGATGGT GGCGTCTTTC
    AGTTATTCTC CCATTCGGCT TCTTGAAGTG TGTGTTCCAC TTCCCAAAAT ATTCATTAAA
    CGTCAAGTCC CACTAAAGGT GTCCCTTCTT CAGGATCTGA AGGACTTCTT TCAGAAAGTT
    TCACAGGTAT ATCTTGCTGT GGATGAAAGA CTTGCGTCTT TGAAAACTGA TACATTTAGC
    AAATCAAGAG AGGAAAAAAT GGAAGATATC TTTGCACAAA AAGAGATGGA AGAAAGTGAG
    TTCAAGAGCT GGACTGAGAA GATGCAGGCG CGGCTCCTGT CTTCCTCCAT GGATACCCCC
    CAGCAACTGC AGTCAGTCTT CGAGTCCCTC ATCGCCAAGA AACAAAGCCT CTGTGAGGTG
    CTGCAAGCTT GGAATAACAG GCTGCAAGAC CTTTTCCAAC AAGAAAAGGG TAGGAAGCGG
    CCTTCAGTTC CTCCAAGTCC TGGAAGACTG AGACAAGGTG AAGAAAGCAA GATAAGTGCA
    GTGGATGCAT CTCCACGGAA TGCTTCTCCA GGACTTCCAA ATGGAGAAAA AGAGGACCGC
    TTCTTAGCCA CCCTTTCCAG TCAGAGCTCC ACCAGTTCCC CCCATCTCCA GCTGCCTACT
    TCTCCTGAAG GCGTGCCTGA GCAGGTCATG GGAGGGCCCC CAGATCTCGA TACTGCCAGC
    AGTTCTGAAG ATGTATTTGA TGGACATTTG CTGGGATCTA CAGACAGCCA GGTGAAGGAA
    AAATCAACCA TGAAAGCCAT CTTTGCAAAT TTGCTTCCAG GAAATAGCTA TAACCCTATT
    CCATTTCCTT TCGATCCAGA TAAACACTAC TTAATGTATG AACATGAACG AGTGCCCATT
    GCAGTCTGTG AGAAGGAGCC CAGCTCCATC ATTGCTTTTG CTCTCAGTTG TAAAGAATAC
    CGGAATGCCT TAGAGGAATT GTCCAAAGCC ACTCAGCGGA ACAGTGCTGA AGAAGGGCTT
    CCAACAAACA GTACTTTAGA CAGCAGACCA AAGAGTAGCA GCCCAATTAG ACTACCTGAG
    ATCAGTGGAG GACAGACAAA CCGTGCGGTA GAAGCAGAAC CACAGCCAAC CAAAAAGGCT
    TCTGGAATGT TGTCCTTCTT CCGAGGGACA GCAGGGAAAA GCCCTGACCT CTCTTCCCAG
    AAGAGAGAGA CCTTACGTGG AGCAGATAGT GCTTACTACC AAGTGGGGCA GACGGGCAAG
    GAGGGGACGG AGAATCAAGG CATTGAGCCT CAAGATGAAG TAGATGGAGG AGATACACAG
    AAGAAACAAC TTACAAATCC TCATGTGGAG CTTCAATTTT CGGATGCCAA CGCCAAGTTT
    TACTGTCGGC TCTACTATGC GGGAGAGTTT CATAAGATGC GAGAAGTGAT TCTGGGCAGC
    AGCGAAGAAG ATTTCATTCG TTCCCTTTCT CACTCTTCTC CGTGGCAGGC CCGAGGAGGC
    AAATCAGGAG CTGCCTTTTA CGCTACTGAA GATGACAGAT TCATTTTGAA GCAAATGCCT
    CGTCTGGAAG TCCAGTCTTT CCTTGACTTC GCACCACACT ACTTCAATTA CATTACAAAT
    GCCGTTCAGC AAAAGAGGCC TACTGCATTG GCCAAGATTC TTGGAGTTTA CAGAATTGGT
    TATAAGAATT CTCAGAACAA CACTGAGAAG AAGCTAGATC TCCTTGTCAT GGAAAATCTT
    TTCTACGGGA GAAAGATGGC ACAGGTTTTT GACTTGAAGG GTTCACTTAG GAATCGAAAT
    GTAAAAACCG ATACCGGGAA AGAGAGCTGT GATGTTGTTC TTCTGGATGA AAATCTCTTA
    AAGATGGTTC GAGACAACCC TCTCTATATC CGTTCTCATT CCAAAGCTGT GCTGAGAGCC
    TCGATCCGGA GCGACTCCCA CTTCCTTTCT AGCCACCTCA TTATAGACTA TTCTTTGCTA
    GTTGGGCGAG ACGATACCAG CAATGAGCTA GTAGTTGGAA TTATAGATTA TATCCGAACA
    TTTACCTGGG ACAAAAAGCT TGAGATGGTT GTGAAATCAA CGGGAATTTT AGGAGGACAA
    GGTAAAATGC CGACTGTGGT CTCTCCAGAG TTGTACAGGA CTAGATTCTG TGAAGCCATG
    GACAAGTATT TCCTGATGGT GCCAGACCAC TGGACAGGCT TGGGTCTGAA CTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834398.1
    CDS155..6451
    Translation

    Target ORF information:

    RefSeq Version XM_024978630.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978630.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGACACCAGA AGGAAGGCAG AACCTACTTT TGGAGGTCAT
    GACCCTCGCA CAGCTGTTCA ACTCCGAAGC CTCAGCACAG TATTAAAACG CCTCAAGGAA
    ATCATGGAGG GGAAGAGCCA GGATAGTGAC CTGAAGCAAT ACTGGATGCC AGATAGCCAG
    TGTAAAGAGT GCTATGACTG TAATGAGAAA TTTACCACTT TTAGGCGCAG ACACCACTGC
    CGACTCTGTG GGCAGATTTT CTGCAGTCGT TGCTGCAATC AAGAAATCCC TGGAAAATTT
    ATGGGTTATA CAGGGGACCT CCGAGCCTGT ACATACTGTA GAAAAATAGC CTTAAGTTAT
    GCTCATTCCA CAGACAGTAA TTCCATTGGT GAAGACTTGA ATGCTCTTTC AGACTCTGCT
    TCCTCTGTGT CTGTACTTGA TCCAAATGAA CCCCGTACAC CTGTGGGGAG TAGAAAAGCC
    AGCCGCAACA TATTTTTAGA GGATGATTTT GCCTGGCAAA GTTTAATTCA TCCAGACTCC
    TCAAACACTG CTCTTTCAAC AAGACTTGTA TCTGTTCAAG AGGATGCTGG GAAATCTCCT
    GCTCGAAATA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGATCTGC GTCTCCAATG
    GTACCATCAT ACGAGACGTC TGTCAGTCCC CAGGCTAACC GAACGTATGT TCGGACAGAG
    ACCACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGTTC AGTTAAAAGA CCTATGGAAG
    AAAATCTGCC ATCACAGCAG TGGAATGGAG TTTCAGGATC ACCGCTACTG GCTGAGAACA
    CACCCCAACT GCATTGTAGG AAAGGAGTTA GTCAACTGGT TAATCCGAAA TGGACACATT
    GCTACAAGGG CACAGGCTAT AGCAATTGGA CAGGCAATGG TGGATGGACG TTGGCTGGAT
    TGTGTCAGTC ACCACGACCA GCTCTTCAGG GATGAGTATG CGCTGTACAG GCCGCTGCAG
    AGTACAGAAT TTTCCGAGAC CCCTTCTCCG GACAGTGACT CGGTGAACTC TGTGGAAGGA
    CACTCTGAGC CATCCTGGTT TAAAGACATC AAGTTTGATG ACAGTGACAC TGAGCAGATA
    GCTGAAGAAG GTGACGATAA TTTGACTAAC TCTGCCAGTC CTAGCAAGCG CACATCAGTC
    AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CCATCAGCCT GAACGTGGAG
    CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC
    CCTGCTGACC AAAAAGAGTA TTTGATTTCT GACAATGGAG GACAACAGCT ATCAATAAGT
    GACGCCTTCA TCAAAGAATC CTTATTTAAC CGTCGAGTTG AGGAAAAATC CAAAGAACTG
    CCTTTCACAC CTTTGGGCTG GCATCATAAC AACCTGGAGC TGCTGAGGGA GGAGAATGGA
    GAGAAACAAG CTATGGAGAG GTTGCTTTCA GCTAATCATA ACCACATGAT GGCGCTGCTC
    CAACAGTTGC TCCATAGTGA GTCATTGTCA ACGTCTTGGA GGGACATCAT CGTATCCCTG
    GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGA ACCGGGATGA TGACATGGAT
    ATCCGCCAGT TTGTCCACAT CAAAAAAATC CCAGGTGGAA AGAAATTTGA TTCTGTGGTT
    GTCAATGGAT TTGTTTGCAC CAAGAATATT GCACATAAAA AGATGAATTC TTCTATTAAA
    AACCCCAAAA TTCTTCTGCT GAAATGTTCC ATTGAATATC TCTACAGAGA AGAAACCAAA
    TTTACTTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCCTAAAGAA TTATGTTCAG
    CGAATAGTTG ATGTTCGACC CACACTGGTT CTGGTTGAGA AAACGGTATC TCGAATTGCA
    CAGGATATGT TGCTGGAACA CGGCATTACT TTGGTCATTA ATGTAAAATC ACAAGTTTTG
    GAACGAGTTA GCCGGATGAC CCAAGGTGAT TTGGTGATGT CAATGGACCA GCTGCTTACC
    AAACCCCACC TGGGCACCTG TCACAAATTT TACATGCAGA TGTTTCAGTT GCCTAATGAA
    CAAACCAAGA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTGGGCTG TACAATCAAG
    CTTAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCTTAAT ATTTATGATC
    TGTGTAGCTT ATCATTCTCA ACTAGAAATC TCCTTCCTTA TGGATGAATT TGCTATGCCT
    CCTACATTAA CGCAAAACCC TTCCTTCCAT TCTCTGATTG AAGGACAGGA GGATGAAGGG
    GCTTCACAGG AGCCATTCAG CGGAAGTCCC CTCCCCCGGG AGCCTGACTT CCCCCCAGAC
    TTTCTGCCTT CTGATGATGG CAGTTTGCTA GAAACAAGGA TTCTGTTTGA GAAGGGTGAC
    CAGGAGAATA AGAGTGTGCC ACAGGATGTT GCCTCTTTGA AGCCTCAAGA GCATGCCCCA
    GCAGCTTGCC CGATGGGTGC CCCCTGTGCT CTCTTTCCAT CAGTACCAGA GTCATTGCTT
    CCGCTCCATG TGGATGACCA ACAGGACGCC ATAGGCAGTG AGCAGCCAGA GGCTTTGCAG
    CAAACAGAGG AGCTTCCGGA TCCCACAAGC CAGATGAGAG CCTTTAGAGA CCCTCTGCAG
    GATGACACTG GCCTGTATGT TACCGAGGAA GTTACCTCTT CTGAAGATAA ACGGAAGACT
    GATTCTTTGA CCTTTAAGCA AGAGTTGAAA GATGTGATCC TCTGTATCTC GCCAGTCATC
    ACATTCCGGG AGCCCTTCCT TTTAACTGAC AAGGGCATGA GATGCTCTAC CCGAGATTAT
    TTTGCGGAGC AGGTTTACTG GTCTCCTCTC CTCAATAAGG AGTTCAAGGA AATGGAGAGC
    AGGCGAAAGA AACAGATGCT TAGGGATCTC TCTGGCCTTC AGGGCATGAA TGGAAGTGTT
    CAGGCCAAGT CCATTCAAGT CCTGCCCTCA CATGAGCTCG TGAGCACCAG AATTGCTGAG
    CACCTGGGGG ACAGCCAGAG CTTGGGGAGA ATGCTAGCCG ATTATCGGGC CAGAGGAGGA
    AGAATCCAGC AAAAAAACTC AGACCCTTTT GCTTACTCAA AGGAGGCATC AAGCACCTCA
    AGTGGAAAAT CAGGAAGCAG AACTGAAGGT GATGAAGAAA AAGGGTTGAT TACCAGTGAT
    GCAGTGTGGT CGACAAAGGT GGACTGTCTG AACCCTGTCA ACCACCAGAG GCTGTGTGTG
    CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC AACGCTCCCA GTGCCTGTGT CAGTCCTTGG
    ATTGTAACAA TGGAATTTTA TGGAAAAAAT GATCTTACAT TAGGAATATT TTTAGAGAGA
    TACTGTTTCC GGCCTTCCTA TCAGTGCCCT AGCATGTTCT GTGATACCCC CATGGTGCAC
    CACATTCGAC GCTTTGTCCA TGGCCAAGGC TGTGTGCAGA TAATCCTAAA GGAGCTGGAT
    TCTCCAGTAC CTGGATATCA GCATACAATT CTCACCTACT CCTGGTGCAG AATGTGCAAA
    CAGGTAACAC CAGTTGTTGC TCTTTCCAAT GAGTCTTGGT CCATGTCGTT TGCAAAATAC
    CTGGAACTTA GGTTTTATGG GCACCAGTAC ACCCGCAGAG CCAATGCTGA GCCATGTGGT
    CACTCCATCC ACCACGATTA TCACCAGTAT TTCTCCTATA ACCAGATGGT GGCGTCTTTC
    AGTTATTCTC CCATTCGGCT TCTTGAAGTG TGTGTTCCAC TTCCCAAAAT ATTCATTAAA
    CGTCAAGTCC CACTAAAGGT GTCCCTTCTT CAGGATCTGA AGGACTTCTT TCAGAAAGTT
    TCACAGGTAT ATCTTGCTGT GGATGAAAGA CTTGCGTCTT TGAAAACTGA TACATTTAGC
    AAATCAAGAG AGGAAAAAAT GGAAGATATC TTTGCACAAA AAGAGATGGA AGAAAGTGAG
    TTCAAGAGCT GGACTGAGAA GATGCAGGCG CGGCTCCTGT CTTCCTCCAT GGATACCCCC
    CAGCAACTGC AGTCAGTCTT CGAGTCCCTC ATCGCCAAGA AACAAAGCCT CTGTGAGGTG
    CTGCAAGCTT GGAATAACAG GCTGCAAGAC CTTTTCCAAC AAGAAAAGGG TAGGAAGCGG
    CCTTCAGTTC CTCCAAGTCC TGGAAGACTG AGACAAGGTG AAGAAAGCAA GATAAGTGCA
    GTGGATGCAT CTCCACGGAA TGCTTCTCCA GGACTTCCAA ATGGAGAAAA AGAGGACCGC
    TTCTTAGCCA CCCTTTCCAG TCAGAGCTCC ACCAGTTCCC CCCATCTCCA GCTGCCTACT
    TCTCCTGAAG GCGTGCCTGA GCAGGTCATG GGAGGGCCCC CAGATCTCGA TACTGCCAGC
    AGTTCTGAAG ATGTATTTGA TGGACATTTG CTGGGATCTA CAGACAGCCA GGTGAAGGAA
    AAATCAACCA TGAAAGCCAT CTTTGCAAAT TTGCTTCCAG GAAATAGCTA TAACCCTATT
    CCATTTCCTT TCGATCCAGA TAAACACTAC TTAATGTATG AACATGAACG AGTGCCCATT
    GCAGTCTGTG AGAAGGAGCC CAGCTCCATC ATTGCTTTTG CTCTCAGTTG TAAAGAATAC
    CGGAATGCCT TAGAGGAATT GTCCAAAGCC ACTCAGCGGA ACAGTGCTGA AGAAGGGCTT
    CCAACAAACA GTACTTTAGA CAGCAGACCA AAGAGTAGCA GCCCAATTAG ACTACCTGAG
    ATCAGTGGAG GACAGACAAA CCGTGCGGTA GAAGCAGAAC CACAGCCAAC CAAAAAGGCT
    TCTGGAATGT TGTCCTTCTT CCGAGGGACA GCAGGGAAAA GCCCTGACCT CTCTTCCCAG
    AAGAGAGAGA CCTTACGTGG AGCAGATAGT GCTTACTACC AAGTGGGGCA GACGGGCAAG
    GAGGGGACGG AGAATCAAGG CATTGAGCCT CAAGATGAAG TAGATGGAGG AGATACACAG
    AAGAAACAAC TTACAAATCC TCATGTGGAG CTTCAATTTT CGGATGCCAA CGCCAAGTTT
    TACTGTCGGC TCTACTATGC GGGAGAGTTT CATAAGATGC GAGAAGTGAT TCTGGGCAGC
    AGCGAAGAAG ATTTCATTCG TTCCCTTTCT CACTCTTCTC CGTGGCAGGC CCGAGGAGGC
    AAATCAGGAG CTGCCTTTTA CGCTACTGAA GATGACAGAT TCATTTTGAA GCAAATGCCT
    CGTCTGGAAG TCCAGTCTTT CCTTGACTTC GCACCACACT ACTTCAATTA CATTACAAAT
    GCCGTTCAGC AAAAGAGGCC TACTGCATTG GCCAAGATTC TTGGAGTTTA CAGAATTGGT
    TATAAGAATT CTCAGAACAA CACTGAGAAG AAGCTAGATC TCCTTGTCAT GGAAAATCTT
    TTCTACGGGA GAAAGATGGC ACAGGTTTTT GACTTGAAGG GTTCACTTAG GAATCGAAAT
    GTAAAAACCG ATACCGGGAA AGAGAGCTGT GATGTTGTTC TTCTGGATGA AAATCTCTTA
    AAGATGGTTC GAGACAACCC TCTCTATATC CGTTCTCATT CCAAAGCTGT GCTGAGAGCC
    TCGATCCGGA GCGACTCCCA CTTCCTTTCT AGCCACCTCA TTATAGACTA TTCTTTGCTA
    GTTGGGCGAG ACGATACCAG CAATGAGCTA GTAGTTGGAA TTATAGATTA TATCCGAACA
    TTTACCTGGG ACAAAAAGCT TGAGATGGTT GTGAAATCAA CGGGAATTTT AGGAGGACAA
    GGTAAAATGC CGACTGTGGT CTCTCCAGAG TTGTACAGGA CTAGATTCTG TGAAGCCATG
    GACAAGTATT TCCTGATGGT GCCAGACCAC TGGACAGGCT TGGGTCTGAA CTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58662
    Clone ID Related Accession (Same CDS sequence) XM_024978618.1 , XM_010802272.2 , XM_010802271.2 , XM_010802273.2 , XM_010802271.3 , XM_010802273.3 , XM_010802272.3
    Accession Version XM_024978618.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6333bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTGGAAC GAGTTAGCCG GATGACCCAA
    GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC
    AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT
    GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG
    CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA
    GAAATCTCCT TCCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC
    TTCCATTCTC TGATTGAAGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA
    AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT
    TTGCTAGAAA CAAGGATTCT GTTTGAGAAG GGTGACCAGG AGAATAAGAG TGTGCCACAG
    GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC
    TGTGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG
    GACGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA CAGAGGAGCT TCCGGATCCC
    ACAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGCCT GTATGTTACC
    GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG
    TTGAAAGATG TGATCCTCTG TATCTCGCCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA
    ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CGGAGCAGGT TTACTGGTCT
    CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GAAAGAAACA GATGCTTAGG
    GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG
    CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG
    GGGAGAATGC TAGCCGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC
    CCTTTTGCTT ACTCAAAGGA GGCATCAAGC ACCTCAAGTG GAAAATCAGG AAGCAGAACT
    GAAGGTGATG AAGAAAAAGG GTTGATTACC AGTGATGCAG TGTGGTCGAC AAAGGTGGAC
    TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG
    TCCAGCAACG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA
    AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG
    TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC
    CAAGGCTGTG TGCAGATAAT CCTAAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT
    ACAATTCTCA CCTACTCCTG GTGCAGAATG TGCAAACAGG TAACACCAGT TGTTGCTCTT
    TCCAATGAGT CTTGGTCCAT GTCGTTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC
    CAGTACACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA CGATTATCAC
    CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT
    GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCACT AAAGGTGTCC
    CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG AAAGTTTCAC AGGTATATCT TGCTGTGGAT
    GAAAGACTTG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA
    GATATCTTTG CACAAAAAGA GATGGAAGAA AGTGAGTTCA AGAGCTGGAC TGAGAAGATG
    CAGGCGCGGC TCCTGTCTTC CTCCATGGAT ACCCCCCAGC AACTGCAGTC AGTCTTCGAG
    TCCCTCATCG CCAAGAAACA AAGCCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG
    CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA
    AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT
    TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT TTCCAGTCAG
    AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGCGT GCCTGAGCAG
    GTCATGGGAG GGCCCCCAGA TCTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA
    CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT
    GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTCGA TCCAGATAAA
    CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC
    TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC
    AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC
    AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT
    GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA
    GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA
    GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT
    GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT
    GTGGAGCTTC AATTTTCGGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA
    GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGCG AAGAAGATTT CATTCGTTCC
    CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT
    ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT
    GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT
    GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT
    GAGAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG
    GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG
    AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTC
    TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC
    CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT
    GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG
    ATGGTTGTGA AATCAACGGG AATTTTAGGA GGACAAGGTA AAATGCCGAC TGTGGTCTCT
    CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA
    GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834386.1
    CDS136..6468
    Translation

    Target ORF information:

    RefSeq Version XM_024978618.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978618.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTGGAAC GAGTTAGCCG GATGACCCAA
    GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC
    AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT
    GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG
    CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA
    GAAATCTCCT TCCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC
    TTCCATTCTC TGATTGAAGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA
    AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT
    TTGCTAGAAA CAAGGATTCT GTTTGAGAAG GGTGACCAGG AGAATAAGAG TGTGCCACAG
    GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC
    TGTGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG
    GACGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA CAGAGGAGCT TCCGGATCCC
    ACAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGCCT GTATGTTACC
    GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG
    TTGAAAGATG TGATCCTCTG TATCTCGCCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA
    ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CGGAGCAGGT TTACTGGTCT
    CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GAAAGAAACA GATGCTTAGG
    GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG
    CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG
    GGGAGAATGC TAGCCGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC
    CCTTTTGCTT ACTCAAAGGA GGCATCAAGC ACCTCAAGTG GAAAATCAGG AAGCAGAACT
    GAAGGTGATG AAGAAAAAGG GTTGATTACC AGTGATGCAG TGTGGTCGAC AAAGGTGGAC
    TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG
    TCCAGCAACG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA
    AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG
    TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC
    CAAGGCTGTG TGCAGATAAT CCTAAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT
    ACAATTCTCA CCTACTCCTG GTGCAGAATG TGCAAACAGG TAACACCAGT TGTTGCTCTT
    TCCAATGAGT CTTGGTCCAT GTCGTTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC
    CAGTACACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA CGATTATCAC
    CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT
    GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCACT AAAGGTGTCC
    CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG AAAGTTTCAC AGGTATATCT TGCTGTGGAT
    GAAAGACTTG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA
    GATATCTTTG CACAAAAAGA GATGGAAGAA AGTGAGTTCA AGAGCTGGAC TGAGAAGATG
    CAGGCGCGGC TCCTGTCTTC CTCCATGGAT ACCCCCCAGC AACTGCAGTC AGTCTTCGAG
    TCCCTCATCG CCAAGAAACA AAGCCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG
    CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA
    AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT
    TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT TTCCAGTCAG
    AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGCGT GCCTGAGCAG
    GTCATGGGAG GGCCCCCAGA TCTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA
    CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT
    GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTCGA TCCAGATAAA
    CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC
    TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC
    AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC
    AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT
    GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA
    GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA
    GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT
    GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT
    GTGGAGCTTC AATTTTCGGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA
    GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGCG AAGAAGATTT CATTCGTTCC
    CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT
    ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT
    GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT
    GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT
    GAGAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG
    GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG
    AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTC
    TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC
    CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT
    GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG
    ATGGTTGTGA AATCAACGGG AATTTTAGGA GGACAAGGTA AAATGCCGAC TGTGGTCTCT
    CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA
    GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58662
    Clone ID Related Accession (Same CDS sequence) XM_024978618.1 , XM_010802272.2 , XM_010802271.2 , XM_010802273.2 , XM_010802271.3 , XM_010802273.3 , XM_010802272.3
    Accession Version XM_010802272.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6333bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802272.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTGGAAC GAGTTAGCCG GATGACCCAA
    GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC
    AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT
    GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG
    CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA
    GAAATCTCCT TCCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC
    TTCCATTCTC TGATTGAAGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA
    AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT
    TTGCTAGAAA CAAGGATTCT GTTTGAGAAG GGTGACCAGG AGAATAAGAG TGTGCCACAG
    GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC
    TGTGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG
    GACGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA CAGAGGAGCT TCCGGATCCC
    ACAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGCCT GTATGTTACC
    GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG
    TTGAAAGATG TGATCCTCTG TATCTCGCCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA
    ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CGGAGCAGGT TTACTGGTCT
    CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GAAAGAAACA GATGCTTAGG
    GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG
    CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG
    GGGAGAATGC TAGCCGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC
    CCTTTTGCTT ACTCAAAGGA GGCATCAAGC ACCTCAAGTG GAAAATCAGG AAGCAGAACT
    GAAGGTGATG AAGAAAAAGG GTTGATTACC AGTGATGCAG TGTGGTCGAC AAAGGTGGAC
    TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG
    TCCAGCAACG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA
    AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG
    TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC
    CAAGGCTGTG TGCAGATAAT CCTAAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT
    ACAATTCTCA CCTACTCCTG GTGCAGAATG TGCAAACAGG TAACACCAGT TGTTGCTCTT
    TCCAATGAGT CTTGGTCCAT GTCGTTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC
    CAGTACACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA CGATTATCAC
    CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT
    GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCACT AAAGGTGTCC
    CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG AAAGTTTCAC AGGTATATCT TGCTGTGGAT
    GAAAGACTTG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA
    GATATCTTTG CACAAAAAGA GATGGAAGAA AGTGAGTTCA AGAGCTGGAC TGAGAAGATG
    CAGGCGCGGC TCCTGTCTTC CTCCATGGAT ACCCCCCAGC AACTGCAGTC AGTCTTCGAG
    TCCCTCATCG CCAAGAAACA AAGCCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG
    CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA
    AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT
    TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT TTCCAGTCAG
    AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGCGT GCCTGAGCAG
    GTCATGGGAG GGCCCCCAGA TCTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA
    CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT
    GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTCGA TCCAGATAAA
    CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC
    TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC
    AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC
    AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT
    GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA
    GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA
    GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT
    GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT
    GTGGAGCTTC AATTTTCGGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA
    GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGCG AAGAAGATTT CATTCGTTCC
    CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT
    ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT
    GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT
    GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT
    GAGAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG
    GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG
    AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTC
    TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC
    CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT
    GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG
    ATGGTTGTGA AATCAACGGG AATTTTAGGA GGACAAGGTA AAATGCCGAC TGTGGTCTCT
    CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA
    GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800574.1
    CDS159..6491
    Translation

    Target ORF information:

    RefSeq Version XM_010802272.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802272.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTGGAAC GAGTTAGCCG GATGACCCAA
    GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC
    AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT
    GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG
    CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA
    GAAATCTCCT TCCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC
    TTCCATTCTC TGATTGAAGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA
    AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT
    TTGCTAGAAA CAAGGATTCT GTTTGAGAAG GGTGACCAGG AGAATAAGAG TGTGCCACAG
    GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC
    TGTGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG
    GACGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA CAGAGGAGCT TCCGGATCCC
    ACAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGCCT GTATGTTACC
    GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG
    TTGAAAGATG TGATCCTCTG TATCTCGCCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA
    ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CGGAGCAGGT TTACTGGTCT
    CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GAAAGAAACA GATGCTTAGG
    GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG
    CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG
    GGGAGAATGC TAGCCGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC
    CCTTTTGCTT ACTCAAAGGA GGCATCAAGC ACCTCAAGTG GAAAATCAGG AAGCAGAACT
    GAAGGTGATG AAGAAAAAGG GTTGATTACC AGTGATGCAG TGTGGTCGAC AAAGGTGGAC
    TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG
    TCCAGCAACG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA
    AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG
    TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC
    CAAGGCTGTG TGCAGATAAT CCTAAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT
    ACAATTCTCA CCTACTCCTG GTGCAGAATG TGCAAACAGG TAACACCAGT TGTTGCTCTT
    TCCAATGAGT CTTGGTCCAT GTCGTTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC
    CAGTACACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA CGATTATCAC
    CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT
    GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCACT AAAGGTGTCC
    CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG AAAGTTTCAC AGGTATATCT TGCTGTGGAT
    GAAAGACTTG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA
    GATATCTTTG CACAAAAAGA GATGGAAGAA AGTGAGTTCA AGAGCTGGAC TGAGAAGATG
    CAGGCGCGGC TCCTGTCTTC CTCCATGGAT ACCCCCCAGC AACTGCAGTC AGTCTTCGAG
    TCCCTCATCG CCAAGAAACA AAGCCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG
    CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA
    AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT
    TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT TTCCAGTCAG
    AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGCGT GCCTGAGCAG
    GTCATGGGAG GGCCCCCAGA TCTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA
    CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT
    GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTCGA TCCAGATAAA
    CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC
    TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC
    AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC
    AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT
    GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA
    GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA
    GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT
    GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT
    GTGGAGCTTC AATTTTCGGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA
    GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGCG AAGAAGATTT CATTCGTTCC
    CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT
    ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT
    GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT
    GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT
    GAGAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG
    GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG
    AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTC
    TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC
    CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT
    GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG
    ATGGTTGTGA AATCAACGGG AATTTTAGGA GGACAAGGTA AAATGCCGAC TGTGGTCTCT
    CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA
    GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58662
    Clone ID Related Accession (Same CDS sequence) XM_024978618.1 , XM_010802272.2 , XM_010802271.2 , XM_010802273.2 , XM_010802271.3 , XM_010802273.3 , XM_010802272.3
    Accession Version XM_010802271.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6333bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802271.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTGGAAC GAGTTAGCCG GATGACCCAA
    GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC
    AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT
    GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG
    CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA
    GAAATCTCCT TCCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC
    TTCCATTCTC TGATTGAAGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA
    AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT
    TTGCTAGAAA CAAGGATTCT GTTTGAGAAG GGTGACCAGG AGAATAAGAG TGTGCCACAG
    GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC
    TGTGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG
    GACGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA CAGAGGAGCT TCCGGATCCC
    ACAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGCCT GTATGTTACC
    GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG
    TTGAAAGATG TGATCCTCTG TATCTCGCCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA
    ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CGGAGCAGGT TTACTGGTCT
    CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GAAAGAAACA GATGCTTAGG
    GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG
    CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG
    GGGAGAATGC TAGCCGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC
    CCTTTTGCTT ACTCAAAGGA GGCATCAAGC ACCTCAAGTG GAAAATCAGG AAGCAGAACT
    GAAGGTGATG AAGAAAAAGG GTTGATTACC AGTGATGCAG TGTGGTCGAC AAAGGTGGAC
    TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG
    TCCAGCAACG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA
    AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG
    TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC
    CAAGGCTGTG TGCAGATAAT CCTAAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT
    ACAATTCTCA CCTACTCCTG GTGCAGAATG TGCAAACAGG TAACACCAGT TGTTGCTCTT
    TCCAATGAGT CTTGGTCCAT GTCGTTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC
    CAGTACACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA CGATTATCAC
    CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT
    GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCACT AAAGGTGTCC
    CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG AAAGTTTCAC AGGTATATCT TGCTGTGGAT
    GAAAGACTTG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA
    GATATCTTTG CACAAAAAGA GATGGAAGAA AGTGAGTTCA AGAGCTGGAC TGAGAAGATG
    CAGGCGCGGC TCCTGTCTTC CTCCATGGAT ACCCCCCAGC AACTGCAGTC AGTCTTCGAG
    TCCCTCATCG CCAAGAAACA AAGCCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG
    CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA
    AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT
    TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT TTCCAGTCAG
    AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGCGT GCCTGAGCAG
    GTCATGGGAG GGCCCCCAGA TCTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA
    CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT
    GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTCGA TCCAGATAAA
    CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC
    TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC
    AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC
    AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT
    GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA
    GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA
    GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT
    GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT
    GTGGAGCTTC AATTTTCGGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA
    GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGCG AAGAAGATTT CATTCGTTCC
    CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT
    ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT
    GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT
    GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT
    GAGAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG
    GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG
    AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTC
    TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC
    CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT
    GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG
    ATGGTTGTGA AATCAACGGG AATTTTAGGA GGACAAGGTA AAATGCCGAC TGTGGTCTCT
    CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA
    GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800573.1
    CDS132..6464
    Translation

    Target ORF information:

    RefSeq Version XM_010802271.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802271.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTGGAAC GAGTTAGCCG GATGACCCAA
    GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC
    AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT
    GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG
    CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA
    GAAATCTCCT TCCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC
    TTCCATTCTC TGATTGAAGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA
    AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT
    TTGCTAGAAA CAAGGATTCT GTTTGAGAAG GGTGACCAGG AGAATAAGAG TGTGCCACAG
    GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC
    TGTGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG
    GACGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA CAGAGGAGCT TCCGGATCCC
    ACAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGCCT GTATGTTACC
    GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG
    TTGAAAGATG TGATCCTCTG TATCTCGCCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA
    ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CGGAGCAGGT TTACTGGTCT
    CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GAAAGAAACA GATGCTTAGG
    GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG
    CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG
    GGGAGAATGC TAGCCGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC
    CCTTTTGCTT ACTCAAAGGA GGCATCAAGC ACCTCAAGTG GAAAATCAGG AAGCAGAACT
    GAAGGTGATG AAGAAAAAGG GTTGATTACC AGTGATGCAG TGTGGTCGAC AAAGGTGGAC
    TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG
    TCCAGCAACG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA
    AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG
    TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC
    CAAGGCTGTG TGCAGATAAT CCTAAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT
    ACAATTCTCA CCTACTCCTG GTGCAGAATG TGCAAACAGG TAACACCAGT TGTTGCTCTT
    TCCAATGAGT CTTGGTCCAT GTCGTTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC
    CAGTACACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA CGATTATCAC
    CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT
    GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCACT AAAGGTGTCC
    CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG AAAGTTTCAC AGGTATATCT TGCTGTGGAT
    GAAAGACTTG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA
    GATATCTTTG CACAAAAAGA GATGGAAGAA AGTGAGTTCA AGAGCTGGAC TGAGAAGATG
    CAGGCGCGGC TCCTGTCTTC CTCCATGGAT ACCCCCCAGC AACTGCAGTC AGTCTTCGAG
    TCCCTCATCG CCAAGAAACA AAGCCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG
    CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA
    AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT
    TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT TTCCAGTCAG
    AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGCGT GCCTGAGCAG
    GTCATGGGAG GGCCCCCAGA TCTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA
    CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT
    GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTCGA TCCAGATAAA
    CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC
    TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC
    AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC
    AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT
    GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA
    GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA
    GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT
    GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT
    GTGGAGCTTC AATTTTCGGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA
    GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGCG AAGAAGATTT CATTCGTTCC
    CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT
    ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT
    GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT
    GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT
    GAGAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG
    GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG
    AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTC
    TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC
    CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT
    GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG
    ATGGTTGTGA AATCAACGGG AATTTTAGGA GGACAAGGTA AAATGCCGAC TGTGGTCTCT
    CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA
    GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58662
    Clone ID Related Accession (Same CDS sequence) XM_024978618.1 , XM_010802272.2 , XM_010802271.2 , XM_010802273.2 , XM_010802271.3 , XM_010802273.3 , XM_010802272.3
    Accession Version XM_010802273.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6333bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802273.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTGGAAC GAGTTAGCCG GATGACCCAA
    GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC
    AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT
    GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG
    CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA
    GAAATCTCCT TCCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC
    TTCCATTCTC TGATTGAAGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA
    AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT
    TTGCTAGAAA CAAGGATTCT GTTTGAGAAG GGTGACCAGG AGAATAAGAG TGTGCCACAG
    GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC
    TGTGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG
    GACGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA CAGAGGAGCT TCCGGATCCC
    ACAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGCCT GTATGTTACC
    GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG
    TTGAAAGATG TGATCCTCTG TATCTCGCCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA
    ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CGGAGCAGGT TTACTGGTCT
    CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GAAAGAAACA GATGCTTAGG
    GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG
    CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG
    GGGAGAATGC TAGCCGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC
    CCTTTTGCTT ACTCAAAGGA GGCATCAAGC ACCTCAAGTG GAAAATCAGG AAGCAGAACT
    GAAGGTGATG AAGAAAAAGG GTTGATTACC AGTGATGCAG TGTGGTCGAC AAAGGTGGAC
    TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG
    TCCAGCAACG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA
    AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG
    TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC
    CAAGGCTGTG TGCAGATAAT CCTAAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT
    ACAATTCTCA CCTACTCCTG GTGCAGAATG TGCAAACAGG TAACACCAGT TGTTGCTCTT
    TCCAATGAGT CTTGGTCCAT GTCGTTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC
    CAGTACACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA CGATTATCAC
    CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT
    GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCACT AAAGGTGTCC
    CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG AAAGTTTCAC AGGTATATCT TGCTGTGGAT
    GAAAGACTTG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA
    GATATCTTTG CACAAAAAGA GATGGAAGAA AGTGAGTTCA AGAGCTGGAC TGAGAAGATG
    CAGGCGCGGC TCCTGTCTTC CTCCATGGAT ACCCCCCAGC AACTGCAGTC AGTCTTCGAG
    TCCCTCATCG CCAAGAAACA AAGCCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG
    CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA
    AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT
    TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT TTCCAGTCAG
    AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGCGT GCCTGAGCAG
    GTCATGGGAG GGCCCCCAGA TCTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA
    CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT
    GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTCGA TCCAGATAAA
    CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC
    TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC
    AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC
    AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT
    GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA
    GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA
    GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT
    GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT
    GTGGAGCTTC AATTTTCGGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA
    GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGCG AAGAAGATTT CATTCGTTCC
    CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT
    ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT
    GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT
    GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT
    GAGAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG
    GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG
    AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTC
    TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC
    CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT
    GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG
    ATGGTTGTGA AATCAACGGG AATTTTAGGA GGACAAGGTA AAATGCCGAC TGTGGTCTCT
    CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA
    GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800575.1
    CDS128..6460
    Translation

    Target ORF information:

    RefSeq Version XM_010802273.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802273.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTGGAAC GAGTTAGCCG GATGACCCAA
    GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC
    AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT
    GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG
    CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA
    GAAATCTCCT TCCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC
    TTCCATTCTC TGATTGAAGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA
    AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT
    TTGCTAGAAA CAAGGATTCT GTTTGAGAAG GGTGACCAGG AGAATAAGAG TGTGCCACAG
    GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC
    TGTGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG
    GACGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA CAGAGGAGCT TCCGGATCCC
    ACAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGCCT GTATGTTACC
    GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG
    TTGAAAGATG TGATCCTCTG TATCTCGCCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA
    ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CGGAGCAGGT TTACTGGTCT
    CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GAAAGAAACA GATGCTTAGG
    GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG
    CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG
    GGGAGAATGC TAGCCGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC
    CCTTTTGCTT ACTCAAAGGA GGCATCAAGC ACCTCAAGTG GAAAATCAGG AAGCAGAACT
    GAAGGTGATG AAGAAAAAGG GTTGATTACC AGTGATGCAG TGTGGTCGAC AAAGGTGGAC
    TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG
    TCCAGCAACG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA
    AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG
    TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC
    CAAGGCTGTG TGCAGATAAT CCTAAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT
    ACAATTCTCA CCTACTCCTG GTGCAGAATG TGCAAACAGG TAACACCAGT TGTTGCTCTT
    TCCAATGAGT CTTGGTCCAT GTCGTTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC
    CAGTACACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA CGATTATCAC
    CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT
    GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCACT AAAGGTGTCC
    CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG AAAGTTTCAC AGGTATATCT TGCTGTGGAT
    GAAAGACTTG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA
    GATATCTTTG CACAAAAAGA GATGGAAGAA AGTGAGTTCA AGAGCTGGAC TGAGAAGATG
    CAGGCGCGGC TCCTGTCTTC CTCCATGGAT ACCCCCCAGC AACTGCAGTC AGTCTTCGAG
    TCCCTCATCG CCAAGAAACA AAGCCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG
    CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA
    AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT
    TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT TTCCAGTCAG
    AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGCGT GCCTGAGCAG
    GTCATGGGAG GGCCCCCAGA TCTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA
    CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT
    GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTCGA TCCAGATAAA
    CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC
    TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC
    AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC
    AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT
    GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA
    GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA
    GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT
    GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT
    GTGGAGCTTC AATTTTCGGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA
    GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGCG AAGAAGATTT CATTCGTTCC
    CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT
    ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT
    GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT
    GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT
    GAGAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG
    GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG
    AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTC
    TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC
    CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT
    GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG
    ATGGTTGTGA AATCAACGGG AATTTTAGGA GGACAAGGTA AAATGCCGAC TGTGGTCTCT
    CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA
    GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58662
    Clone ID Related Accession (Same CDS sequence) XM_024978618.1 , XM_010802272.2 , XM_010802271.2 , XM_010802273.2 , XM_010802271.3 , XM_010802273.3 , XM_010802272.3
    Accession Version XM_010802271.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6333bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802271.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTGGAAC GAGTTAGCCG GATGACCCAA
    GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC
    AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT
    GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG
    CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA
    GAAATCTCCT TCCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC
    TTCCATTCTC TGATTGAAGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA
    AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT
    TTGCTAGAAA CAAGGATTCT GTTTGAGAAG GGTGACCAGG AGAATAAGAG TGTGCCACAG
    GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC
    TGTGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG
    GACGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA CAGAGGAGCT TCCGGATCCC
    ACAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGCCT GTATGTTACC
    GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG
    TTGAAAGATG TGATCCTCTG TATCTCGCCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA
    ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CGGAGCAGGT TTACTGGTCT
    CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GAAAGAAACA GATGCTTAGG
    GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG
    CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG
    GGGAGAATGC TAGCCGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC
    CCTTTTGCTT ACTCAAAGGA GGCATCAAGC ACCTCAAGTG GAAAATCAGG AAGCAGAACT
    GAAGGTGATG AAGAAAAAGG GTTGATTACC AGTGATGCAG TGTGGTCGAC AAAGGTGGAC
    TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG
    TCCAGCAACG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA
    AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG
    TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC
    CAAGGCTGTG TGCAGATAAT CCTAAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT
    ACAATTCTCA CCTACTCCTG GTGCAGAATG TGCAAACAGG TAACACCAGT TGTTGCTCTT
    TCCAATGAGT CTTGGTCCAT GTCGTTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC
    CAGTACACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA CGATTATCAC
    CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT
    GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCACT AAAGGTGTCC
    CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG AAAGTTTCAC AGGTATATCT TGCTGTGGAT
    GAAAGACTTG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA
    GATATCTTTG CACAAAAAGA GATGGAAGAA AGTGAGTTCA AGAGCTGGAC TGAGAAGATG
    CAGGCGCGGC TCCTGTCTTC CTCCATGGAT ACCCCCCAGC AACTGCAGTC AGTCTTCGAG
    TCCCTCATCG CCAAGAAACA AAGCCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG
    CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA
    AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT
    TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT TTCCAGTCAG
    AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGCGT GCCTGAGCAG
    GTCATGGGAG GGCCCCCAGA TCTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA
    CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT
    GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTCGA TCCAGATAAA
    CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC
    TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC
    AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC
    AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT
    GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA
    GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA
    GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT
    GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT
    GTGGAGCTTC AATTTTCGGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA
    GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGCG AAGAAGATTT CATTCGTTCC
    CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT
    ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT
    GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT
    GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT
    GAGAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG
    GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG
    AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTC
    TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC
    CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT
    GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG
    ATGGTTGTGA AATCAACGGG AATTTTAGGA GGACAAGGTA AAATGCCGAC TGTGGTCTCT
    CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA
    GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800573.1
    CDS149..6481
    Translation

    Target ORF information:

    RefSeq Version XM_010802271.3
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802271.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTGGAAC GAGTTAGCCG GATGACCCAA
    GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC
    AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT
    GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG
    CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA
    GAAATCTCCT TCCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC
    TTCCATTCTC TGATTGAAGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA
    AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT
    TTGCTAGAAA CAAGGATTCT GTTTGAGAAG GGTGACCAGG AGAATAAGAG TGTGCCACAG
    GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC
    TGTGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG
    GACGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA CAGAGGAGCT TCCGGATCCC
    ACAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGCCT GTATGTTACC
    GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG
    TTGAAAGATG TGATCCTCTG TATCTCGCCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA
    ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CGGAGCAGGT TTACTGGTCT
    CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GAAAGAAACA GATGCTTAGG
    GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG
    CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG
    GGGAGAATGC TAGCCGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC
    CCTTTTGCTT ACTCAAAGGA GGCATCAAGC ACCTCAAGTG GAAAATCAGG AAGCAGAACT
    GAAGGTGATG AAGAAAAAGG GTTGATTACC AGTGATGCAG TGTGGTCGAC AAAGGTGGAC
    TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG
    TCCAGCAACG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA
    AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG
    TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC
    CAAGGCTGTG TGCAGATAAT CCTAAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT
    ACAATTCTCA CCTACTCCTG GTGCAGAATG TGCAAACAGG TAACACCAGT TGTTGCTCTT
    TCCAATGAGT CTTGGTCCAT GTCGTTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC
    CAGTACACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA CGATTATCAC
    CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT
    GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCACT AAAGGTGTCC
    CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG AAAGTTTCAC AGGTATATCT TGCTGTGGAT
    GAAAGACTTG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA
    GATATCTTTG CACAAAAAGA GATGGAAGAA AGTGAGTTCA AGAGCTGGAC TGAGAAGATG
    CAGGCGCGGC TCCTGTCTTC CTCCATGGAT ACCCCCCAGC AACTGCAGTC AGTCTTCGAG
    TCCCTCATCG CCAAGAAACA AAGCCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG
    CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA
    AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT
    TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT TTCCAGTCAG
    AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGCGT GCCTGAGCAG
    GTCATGGGAG GGCCCCCAGA TCTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA
    CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT
    GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTCGA TCCAGATAAA
    CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC
    TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC
    AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC
    AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT
    GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA
    GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA
    GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT
    GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT
    GTGGAGCTTC AATTTTCGGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA
    GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGCG AAGAAGATTT CATTCGTTCC
    CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT
    ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT
    GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT
    GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT
    GAGAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG
    GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG
    AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTC
    TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC
    CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT
    GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG
    ATGGTTGTGA AATCAACGGG AATTTTAGGA GGACAAGGTA AAATGCCGAC TGTGGTCTCT
    CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA
    GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58662
    Clone ID Related Accession (Same CDS sequence) XM_024978618.1 , XM_010802272.2 , XM_010802271.2 , XM_010802273.2 , XM_010802271.3 , XM_010802273.3 , XM_010802272.3
    Accession Version XM_010802273.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6333bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802273.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTGGAAC GAGTTAGCCG GATGACCCAA
    GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC
    AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT
    GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG
    CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA
    GAAATCTCCT TCCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC
    TTCCATTCTC TGATTGAAGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA
    AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT
    TTGCTAGAAA CAAGGATTCT GTTTGAGAAG GGTGACCAGG AGAATAAGAG TGTGCCACAG
    GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC
    TGTGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG
    GACGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA CAGAGGAGCT TCCGGATCCC
    ACAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGCCT GTATGTTACC
    GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG
    TTGAAAGATG TGATCCTCTG TATCTCGCCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA
    ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CGGAGCAGGT TTACTGGTCT
    CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GAAAGAAACA GATGCTTAGG
    GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG
    CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG
    GGGAGAATGC TAGCCGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC
    CCTTTTGCTT ACTCAAAGGA GGCATCAAGC ACCTCAAGTG GAAAATCAGG AAGCAGAACT
    GAAGGTGATG AAGAAAAAGG GTTGATTACC AGTGATGCAG TGTGGTCGAC AAAGGTGGAC
    TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG
    TCCAGCAACG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA
    AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG
    TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC
    CAAGGCTGTG TGCAGATAAT CCTAAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT
    ACAATTCTCA CCTACTCCTG GTGCAGAATG TGCAAACAGG TAACACCAGT TGTTGCTCTT
    TCCAATGAGT CTTGGTCCAT GTCGTTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC
    CAGTACACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA CGATTATCAC
    CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT
    GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCACT AAAGGTGTCC
    CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG AAAGTTTCAC AGGTATATCT TGCTGTGGAT
    GAAAGACTTG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA
    GATATCTTTG CACAAAAAGA GATGGAAGAA AGTGAGTTCA AGAGCTGGAC TGAGAAGATG
    CAGGCGCGGC TCCTGTCTTC CTCCATGGAT ACCCCCCAGC AACTGCAGTC AGTCTTCGAG
    TCCCTCATCG CCAAGAAACA AAGCCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG
    CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA
    AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT
    TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT TTCCAGTCAG
    AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGCGT GCCTGAGCAG
    GTCATGGGAG GGCCCCCAGA TCTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA
    CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT
    GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTCGA TCCAGATAAA
    CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC
    TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC
    AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC
    AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT
    GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA
    GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA
    GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT
    GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT
    GTGGAGCTTC AATTTTCGGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA
    GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGCG AAGAAGATTT CATTCGTTCC
    CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT
    ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT
    GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT
    GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT
    GAGAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG
    GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG
    AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTC
    TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC
    CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT
    GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG
    ATGGTTGTGA AATCAACGGG AATTTTAGGA GGACAAGGTA AAATGCCGAC TGTGGTCTCT
    CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA
    GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800575.1
    CDS138..6470
    Translation

    Target ORF information:

    RefSeq Version XM_010802273.3
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802273.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTGGAAC GAGTTAGCCG GATGACCCAA
    GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC
    AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT
    GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG
    CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA
    GAAATCTCCT TCCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC
    TTCCATTCTC TGATTGAAGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA
    AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT
    TTGCTAGAAA CAAGGATTCT GTTTGAGAAG GGTGACCAGG AGAATAAGAG TGTGCCACAG
    GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC
    TGTGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG
    GACGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA CAGAGGAGCT TCCGGATCCC
    ACAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGCCT GTATGTTACC
    GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG
    TTGAAAGATG TGATCCTCTG TATCTCGCCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA
    ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CGGAGCAGGT TTACTGGTCT
    CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GAAAGAAACA GATGCTTAGG
    GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG
    CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG
    GGGAGAATGC TAGCCGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC
    CCTTTTGCTT ACTCAAAGGA GGCATCAAGC ACCTCAAGTG GAAAATCAGG AAGCAGAACT
    GAAGGTGATG AAGAAAAAGG GTTGATTACC AGTGATGCAG TGTGGTCGAC AAAGGTGGAC
    TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG
    TCCAGCAACG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA
    AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG
    TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC
    CAAGGCTGTG TGCAGATAAT CCTAAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT
    ACAATTCTCA CCTACTCCTG GTGCAGAATG TGCAAACAGG TAACACCAGT TGTTGCTCTT
    TCCAATGAGT CTTGGTCCAT GTCGTTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC
    CAGTACACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA CGATTATCAC
    CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT
    GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCACT AAAGGTGTCC
    CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG AAAGTTTCAC AGGTATATCT TGCTGTGGAT
    GAAAGACTTG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA
    GATATCTTTG CACAAAAAGA GATGGAAGAA AGTGAGTTCA AGAGCTGGAC TGAGAAGATG
    CAGGCGCGGC TCCTGTCTTC CTCCATGGAT ACCCCCCAGC AACTGCAGTC AGTCTTCGAG
    TCCCTCATCG CCAAGAAACA AAGCCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG
    CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA
    AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT
    TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT TTCCAGTCAG
    AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGCGT GCCTGAGCAG
    GTCATGGGAG GGCCCCCAGA TCTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA
    CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT
    GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTCGA TCCAGATAAA
    CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC
    TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC
    AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC
    AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT
    GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA
    GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA
    GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT
    GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT
    GTGGAGCTTC AATTTTCGGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA
    GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGCG AAGAAGATTT CATTCGTTCC
    CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT
    ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT
    GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT
    GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT
    GAGAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG
    GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG
    AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTC
    TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC
    CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT
    GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG
    ATGGTTGTGA AATCAACGGG AATTTTAGGA GGACAAGGTA AAATGCCGAC TGTGGTCTCT
    CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA
    GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58662
    Clone ID Related Accession (Same CDS sequence) XM_024978618.1 , XM_010802272.2 , XM_010802271.2 , XM_010802273.2 , XM_010802271.3 , XM_010802273.3 , XM_010802272.3
    Accession Version XM_010802272.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6333bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802272.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTGGAAC GAGTTAGCCG GATGACCCAA
    GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC
    AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT
    GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG
    CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA
    GAAATCTCCT TCCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC
    TTCCATTCTC TGATTGAAGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA
    AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT
    TTGCTAGAAA CAAGGATTCT GTTTGAGAAG GGTGACCAGG AGAATAAGAG TGTGCCACAG
    GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC
    TGTGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG
    GACGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA CAGAGGAGCT TCCGGATCCC
    ACAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGCCT GTATGTTACC
    GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG
    TTGAAAGATG TGATCCTCTG TATCTCGCCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA
    ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CGGAGCAGGT TTACTGGTCT
    CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GAAAGAAACA GATGCTTAGG
    GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG
    CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG
    GGGAGAATGC TAGCCGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC
    CCTTTTGCTT ACTCAAAGGA GGCATCAAGC ACCTCAAGTG GAAAATCAGG AAGCAGAACT
    GAAGGTGATG AAGAAAAAGG GTTGATTACC AGTGATGCAG TGTGGTCGAC AAAGGTGGAC
    TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG
    TCCAGCAACG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA
    AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG
    TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC
    CAAGGCTGTG TGCAGATAAT CCTAAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT
    ACAATTCTCA CCTACTCCTG GTGCAGAATG TGCAAACAGG TAACACCAGT TGTTGCTCTT
    TCCAATGAGT CTTGGTCCAT GTCGTTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC
    CAGTACACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA CGATTATCAC
    CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT
    GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCACT AAAGGTGTCC
    CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG AAAGTTTCAC AGGTATATCT TGCTGTGGAT
    GAAAGACTTG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA
    GATATCTTTG CACAAAAAGA GATGGAAGAA AGTGAGTTCA AGAGCTGGAC TGAGAAGATG
    CAGGCGCGGC TCCTGTCTTC CTCCATGGAT ACCCCCCAGC AACTGCAGTC AGTCTTCGAG
    TCCCTCATCG CCAAGAAACA AAGCCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG
    CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA
    AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT
    TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT TTCCAGTCAG
    AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGCGT GCCTGAGCAG
    GTCATGGGAG GGCCCCCAGA TCTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA
    CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT
    GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTCGA TCCAGATAAA
    CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC
    TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC
    AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC
    AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT
    GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA
    GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA
    GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT
    GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT
    GTGGAGCTTC AATTTTCGGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA
    GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGCG AAGAAGATTT CATTCGTTCC
    CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT
    ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT
    GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT
    GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT
    GAGAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG
    GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG
    AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTC
    TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC
    CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT
    GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG
    ATGGTTGTGA AATCAACGGG AATTTTAGGA GGACAAGGTA AAATGCCGAC TGTGGTCTCT
    CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA
    GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800574.1
    CDS155..6487
    Translation

    Target ORF information:

    RefSeq Version XM_010802272.3
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802272.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTGGAAC GAGTTAGCCG GATGACCCAA
    GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC
    AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT
    GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG
    CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA
    GAAATCTCCT TCCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC
    TTCCATTCTC TGATTGAAGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA
    AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT
    TTGCTAGAAA CAAGGATTCT GTTTGAGAAG GGTGACCAGG AGAATAAGAG TGTGCCACAG
    GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC
    TGTGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG
    GACGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA CAGAGGAGCT TCCGGATCCC
    ACAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGCCT GTATGTTACC
    GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG
    TTGAAAGATG TGATCCTCTG TATCTCGCCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA
    ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CGGAGCAGGT TTACTGGTCT
    CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GAAAGAAACA GATGCTTAGG
    GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG
    CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG
    GGGAGAATGC TAGCCGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC
    CCTTTTGCTT ACTCAAAGGA GGCATCAAGC ACCTCAAGTG GAAAATCAGG AAGCAGAACT
    GAAGGTGATG AAGAAAAAGG GTTGATTACC AGTGATGCAG TGTGGTCGAC AAAGGTGGAC
    TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG
    TCCAGCAACG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA
    AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG
    TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC
    CAAGGCTGTG TGCAGATAAT CCTAAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT
    ACAATTCTCA CCTACTCCTG GTGCAGAATG TGCAAACAGG TAACACCAGT TGTTGCTCTT
    TCCAATGAGT CTTGGTCCAT GTCGTTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC
    CAGTACACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA CGATTATCAC
    CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT
    GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCACT AAAGGTGTCC
    CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG AAAGTTTCAC AGGTATATCT TGCTGTGGAT
    GAAAGACTTG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA
    GATATCTTTG CACAAAAAGA GATGGAAGAA AGTGAGTTCA AGAGCTGGAC TGAGAAGATG
    CAGGCGCGGC TCCTGTCTTC CTCCATGGAT ACCCCCCAGC AACTGCAGTC AGTCTTCGAG
    TCCCTCATCG CCAAGAAACA AAGCCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG
    CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA
    AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT
    TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT TTCCAGTCAG
    AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGCGT GCCTGAGCAG
    GTCATGGGAG GGCCCCCAGA TCTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA
    CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT
    GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTCGA TCCAGATAAA
    CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC
    TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC
    AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC
    AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT
    GCGGTAGAAG CAGAACCACA GCCAACCAAA AAGGCTTCTG GAATGTTGTC CTTCTTCCGA
    GGGACAGCAG GGAAAAGCCC TGACCTCTCT TCCCAGAAGA GAGAGACCTT ACGTGGAGCA
    GATAGTGCTT ACTACCAAGT GGGGCAGACG GGCAAGGAGG GGACGGAGAA TCAAGGCATT
    GAGCCTCAAG ATGAAGTAGA TGGAGGAGAT ACACAGAAGA AACAACTTAC AAATCCTCAT
    GTGGAGCTTC AATTTTCGGA TGCCAACGCC AAGTTTTACT GTCGGCTCTA CTATGCGGGA
    GAGTTTCATA AGATGCGAGA AGTGATTCTG GGCAGCAGCG AAGAAGATTT CATTCGTTCC
    CTTTCTCACT CTTCTCCGTG GCAGGCCCGA GGAGGCAAAT CAGGAGCTGC CTTTTACGCT
    ACTGAAGATG ACAGATTCAT TTTGAAGCAA ATGCCTCGTC TGGAAGTCCA GTCTTTCCTT
    GACTTCGCAC CACACTACTT CAATTACATT ACAAATGCCG TTCAGCAAAA GAGGCCTACT
    GCATTGGCCA AGATTCTTGG AGTTTACAGA ATTGGTTATA AGAATTCTCA GAACAACACT
    GAGAAGAAGC TAGATCTCCT TGTCATGGAA AATCTTTTCT ACGGGAGAAA GATGGCACAG
    GTTTTTGACT TGAAGGGTTC ACTTAGGAAT CGAAATGTAA AAACCGATAC CGGGAAAGAG
    AGCTGTGATG TTGTTCTTCT GGATGAAAAT CTCTTAAAGA TGGTTCGAGA CAACCCTCTC
    TATATCCGTT CTCATTCCAA AGCTGTGCTG AGAGCCTCGA TCCGGAGCGA CTCCCACTTC
    CTTTCTAGCC ACCTCATTAT AGACTATTCT TTGCTAGTTG GGCGAGACGA TACCAGCAAT
    GAGCTAGTAG TTGGAATTAT AGATTATATC CGAACATTTA CCTGGGACAA AAAGCTTGAG
    ATGGTTGTGA AATCAACGGG AATTTTAGGA GGACAAGGTA AAATGCCGAC TGTGGTCTCT
    CCAGAGTTGT ACAGGACTAG ATTCTGTGAA GCCATGGACA AGTATTTCCT GATGGTGCCA
    GACCACTGGA CAGGCTTGGG TCTGAACTGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58663
    Clone ID Related Accession (Same CDS sequence) XM_024978627.1 , XM_010802274.2
    Accession Version XM_024978627.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6330bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGAGGACAGT TTGCAACACC CCCAGGAGAA CTCAGACACC
    AGAAGGAAGG CAGAACCTAC TTTTGGAGGT CATGACCCTC GCACAGCTGT TCAACTCCGA
    AGCCTCAGCA CAGTATTAAA ACGCCTCAAG GAAATCATGG AGGGGAAGAG CCAGGATAGT
    GACCTGAAGC AATACTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGTAATGAG
    AAATTTACCA CTTTTAGGCG CAGACACCAC TGCCGACTCT GTGGGCAGAT TTTCTGCAGT
    CGTTGCTGCA ATCAAGAAAT CCCTGGAAAA TTTATGGGTT ATACAGGGGA CCTCCGAGCC
    TGTACATACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCCATT
    GGTGAAGACT TGAATGCTCT TTCAGACTCT GCTTCCTCTG TGTCTGTACT TGATCCAAAT
    GAACCCCGTA CACCTGTGGG GAGTAGAAAA GCCAGCCGCA ACATATTTTT AGAGGATGAT
    TTTGCCTGGC AAAGTTTAAT TCATCCAGAC TCCTCAAACA CTGCTCTTTC AACAAGACTT
    GTATCTGTTC AAGAGGATGC TGGGAAATCT CCTGCTCGAA ATAGATCAGC CAGCATTACT
    AATCTGTCAC TGGATCGATC TGCGTCTCCA ATGGTACCAT CATACGAGAC GTCTGTCAGT
    CCCCAGGCTA ACCGAACGTA TGTTCGGACA GAGACCACTG AGGATGAACG CAAAATTCTT
    CTGGACAGTG TTCAGTTAAA AGACCTATGG AAGAAAATCT GCCATCACAG CAGTGGAATG
    GAGTTTCAGG ATCACCGCTA CTGGCTGAGA ACACACCCCA ACTGCATTGT AGGAAAGGAG
    TTAGTCAACT GGTTAATCCG AAATGGACAC ATTGCTACAA GGGCACAGGC TATAGCAATT
    GGACAGGCAA TGGTGGATGG ACGTTGGCTG GATTGTGTCA GTCACCACGA CCAGCTCTTC
    AGGGATGAGT ATGCGCTGTA CAGGCCGCTG CAGAGTACAG AATTTTCCGA GACCCCTTCT
    CCGGACAGTG ACTCGGTGAA CTCTGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC
    ATCAAGTTTG ATGACAGTGA CACTGAGCAG ATAGCTGAAG AAGGTGACGA TAATTTGACT
    AACTCTGCCA GTCCTAGCAA GCGCACATCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT
    GACTCAGCCG CTTCCATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG
    AAGCCCTCCA AGTACCCACA TGTGCCCCCT CACCCTGCTG ACCAAAAAGA GTATTTGATT
    TCTGACAATG GAGGACAACA GCTATCAATA AGTGACGCCT TCATCAAAGA ATCCTTATTT
    AACCGTCGAG TTGAGGAAAA ATCCAAAGAA CTGCCTTTCA CACCTTTGGG CTGGCATCAT
    AACAACCTGG AGCTGCTGAG GGAGGAGAAT GGAGAGAAAC AAGCTATGGA GAGGTTGCTT
    TCAGCTAATC ATAACCACAT GATGGCGCTG CTCCAACAGT TGCTCCATAG TGAGTCATTG
    TCAACGTCTT GGAGGGACAT CATCGTATCC CTGGTCTGCC AGGTTGTTCA GACAGTCCGA
    CCTGACGTCA AGAACCGGGA TGATGACATG GATATCCGCC AGTTTGTCCA CATCAAAAAA
    ATCCCAGGTG GAAAGAAATT TGATTCTGTG GTTGTCAATG GATTTGTTTG CACCAAGAAT
    ATTGCACATA AAAAGATGAA TTCTTCTATT AAAAACCCCA AAATTCTTCT GCTGAAATGT
    TCCATTGAAT ATCTCTACAG AGAAGAAACC AAATTTACTT GCATTGATCC TATTGTGCTT
    CAGGAAAGGG AATTCCTAAA GAATTATGTT CAGCGAATAG TTGATGTTCG ACCCACACTG
    GTTCTGGTTG AGAAAACGGT ATCTCGAATT GCACAGGATA TGTTGCTGGA ACACGGCATT
    ACTTTGGTCA TTAATGTAAA ATCACAAGTT TTGGAACGAG TTAGCCGGAT GACCCAAGGT
    GATTTGGTGA TGTCAATGGA CCAGCTGCTT ACCAAACCCC ACCTGGGCAC CTGTCACAAA
    TTTTACATGC AGATGTTTCA GTTGCCTAAT GAACAAACCA AGACACTGAT GTTTTTTGAA
    GGCTGTCCAC AGCACCTGGG CTGTACAATC AAGCTTAGAG GAGGCTCTGA TTATGAGCTG
    GCTCGAGTTA AGGAGATCTT AATATTTATG ATCTGTGTAG CTTATCATTC TCAACTAGAA
    ATCTCCTTCC TTATGGATGA ATTTGCTATG CCTCCTACAT TAACGCAAAA CCCTTCCTTC
    CATTCTCTGA TTGAAGGACA GGAGGATGAA GGGGCTTCAC AGGAGCCATT CAGCGGAAGT
    CCCCTCCCCC GGGAGCCTGA CTTCCCCCCA GACTTTCTGC CTTCTGATGA TGGCAGTTTG
    CTAGAAACAA GGATTCTGTT TGAGAAGGGT GACCAGGAGA ATAAGAGTGT GCCACAGGAT
    GTTGCCTCTT TGAAGCCTCA AGAGCATGCC CCAGCAGCTT GCCCGATGGG TGCCCCCTGT
    GCTCTCTTTC CATCAGTACC AGAGTCATTG CTTCCGCTCC ATGTGGATGA CCAACAGGAC
    GCCATAGGCA GTGAGCAGCC AGAGGCTTTG CAGCAAACAG AGGAGCTTCC GGATCCCACA
    AGCCAGATGA GAGCCTTTAG AGACCCTCTG CAGGATGACA CTGGCCTGTA TGTTACCGAG
    GAAGTTACCT CTTCTGAAGA TAAACGGAAG ACTGATTCTT TGACCTTTAA GCAAGAGTTG
    AAAGATGTGA TCCTCTGTAT CTCGCCAGTC ATCACATTCC GGGAGCCCTT CCTTTTAACT
    GACAAGGGCA TGAGATGCTC TACCCGAGAT TATTTTGCGG AGCAGGTTTA CTGGTCTCCT
    CTCCTCAATA AGGAGTTCAA GGAAATGGAG AGCAGGCGAA AGAAACAGAT GCTTAGGGAT
    CTCTCTGGCC TTCAGGGCAT GAATGGAAGT GTTCAGGCCA AGTCCATTCA AGTCCTGCCC
    TCACATGAGC TCGTGAGCAC CAGAATTGCT GAGCACCTGG GGGACAGCCA GAGCTTGGGG
    AGAATGCTAG CCGATTATCG GGCCAGAGGA GGAAGAATCC AGCAAAAAAA CTCAGACCCT
    TTTGCTTACT CAAAGGAGGC ATCAAGCACC TCAAGTGGAA AATCAGGAAG CAGAACTGAA
    GGTGATGAAG AAAAAGGGTT GATTACCAGT GATGCAGTGT GGTCGACAAA GGTGGACTGT
    CTGAACCCTG TCAACCACCA GAGGCTGTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC
    AGCAACGCTC CCAGTGCCTG TGTCAGTCCT TGGATTGTAA CAATGGAATT TTATGGAAAA
    AATGATCTTA CATTAGGAAT ATTTTTAGAG AGATACTGTT TCCGGCCTTC CTATCAGTGC
    CCTAGCATGT TCTGTGATAC CCCCATGGTG CACCACATTC GACGCTTTGT CCATGGCCAA
    GGCTGTGTGC AGATAATCCT AAAGGAGCTG GATTCTCCAG TACCTGGATA TCAGCATACA
    ATTCTCACCT ACTCCTGGTG CAGAATGTGC AAACAGGTAA CACCAGTTGT TGCTCTTTCC
    AATGAGTCTT GGTCCATGTC GTTTGCAAAA TACCTGGAAC TTAGGTTTTA TGGGCACCAG
    TACACCCGCA GAGCCAATGC TGAGCCATGT GGTCACTCCA TCCACCACGA TTATCACCAG
    TATTTCTCCT ATAACCAGAT GGTGGCGTCT TTCAGTTATT CTCCCATTCG GCTTCTTGAA
    GTGTGTGTTC CACTTCCCAA AATATTCATT AAACGTCAAG TCCCACTAAA GGTGTCCCTT
    CTTCAGGATC TGAAGGACTT CTTTCAGAAA GTTTCACAGG TATATCTTGC TGTGGATGAA
    AGACTTGCGT CTTTGAAAAC TGATACATTT AGCAAATCAA GAGAGGAAAA AATGGAAGAT
    ATCTTTGCAC AAAAAGAGAT GGAAGAAAGT GAGTTCAAGA GCTGGACTGA GAAGATGCAG
    GCGCGGCTCC TGTCTTCCTC CATGGATACC CCCCAGCAAC TGCAGTCAGT CTTCGAGTCC
    CTCATCGCCA AGAAACAAAG CCTCTGTGAG GTGCTGCAAG CTTGGAATAA CAGGCTGCAA
    GACCTTTTCC AACAAGAAAA GGGTAGGAAG CGGCCTTCAG TTCCTCCAAG TCCTGGAAGA
    CTGAGACAAG GTGAAGAAAG CAAGATAAGT GCAGTGGATG CATCTCCACG GAATGCTTCT
    CCAGGACTTC CAAATGGAGA AAAAGAGGAC CGCTTCTTAG CCACCCTTTC CAGTCAGAGC
    TCCACCAGTT CCCCCCATCT CCAGCTGCCT ACTTCTCCTG AAGGCGTGCC TGAGCAGGTC
    ATGGGAGGGC CCCCAGATCT CGATACTGCC AGCAGTTCTG AAGATGTATT TGATGGACAT
    TTGCTGGGAT CTACAGACAG CCAGGTGAAG GAAAAATCAA CCATGAAAGC CATCTTTGCA
    AATTTGCTTC CAGGAAATAG CTATAACCCT ATTCCATTTC CTTTCGATCC AGATAAACAC
    TACTTAATGT ATGAACATGA ACGAGTGCCC ATTGCAGTCT GTGAGAAGGA GCCCAGCTCC
    ATCATTGCTT TTGCTCTCAG TTGTAAAGAA TACCGGAATG CCTTAGAGGA ATTGTCCAAA
    GCCACTCAGC GGAACAGTGC TGAAGAAGGG CTTCCAACAA ACAGTACTTT AGACAGCAGA
    CCAAAGAGTA GCAGCCCAAT TAGACTACCT GAGATCAGTG GAGGACAGAC AAACCGTGCG
    GTAGAAGCAG AACCACAGCC AACCAAAAAG GCTTCTGGAA TGTTGTCCTT CTTCCGAGGG
    ACAGCAGGGA AAAGCCCTGA CCTCTCTTCC CAGAAGAGAG AGACCTTACG TGGAGCAGAT
    AGTGCTTACT ACCAAGTGGG GCAGACGGGC AAGGAGGGGA CGGAGAATCA AGGCATTGAG
    CCTCAAGATG AAGTAGATGG AGGAGATACA CAGAAGAAAC AACTTACAAA TCCTCATGTG
    GAGCTTCAAT TTTCGGATGC CAACGCCAAG TTTTACTGTC GGCTCTACTA TGCGGGAGAG
    TTTCATAAGA TGCGAGAAGT GATTCTGGGC AGCAGCGAAG AAGATTTCAT TCGTTCCCTT
    TCTCACTCTT CTCCGTGGCA GGCCCGAGGA GGCAAATCAG GAGCTGCCTT TTACGCTACT
    GAAGATGACA GATTCATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC
    TTCGCACCAC ACTACTTCAA TTACATTACA AATGCCGTTC AGCAAAAGAG GCCTACTGCA
    TTGGCCAAGA TTCTTGGAGT TTACAGAATT GGTTATAAGA ATTCTCAGAA CAACACTGAG
    AAGAAGCTAG ATCTCCTTGT CATGGAAAAT CTTTTCTACG GGAGAAAGAT GGCACAGGTT
    TTTGACTTGA AGGGTTCACT TAGGAATCGA AATGTAAAAA CCGATACCGG GAAAGAGAGC
    TGTGATGTTG TTCTTCTGGA TGAAAATCTC TTAAAGATGG TTCGAGACAA CCCTCTCTAT
    ATCCGTTCTC ATTCCAAAGC TGTGCTGAGA GCCTCGATCC GGAGCGACTC CCACTTCCTT
    TCTAGCCACC TCATTATAGA CTATTCTTTG CTAGTTGGGC GAGACGATAC CAGCAATGAG
    CTAGTAGTTG GAATTATAGA TTATATCCGA ACATTTACCT GGGACAAAAA GCTTGAGATG
    GTTGTGAAAT CAACGGGAAT TTTAGGAGGA CAAGGTAAAA TGCCGACTGT GGTCTCTCCA
    GAGTTGTACA GGACTAGATT CTGTGAAGCC ATGGACAAGT ATTTCCTGAT GGTGCCAGAC
    CACTGGACAG GCTTGGGTCT GAACTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834395.1
    CDS155..6484
    Translation

    Target ORF information:

    RefSeq Version XM_024978627.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978627.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGAGGACAGT TTGCAACACC CCCAGGAGAA CTCAGACACC
    AGAAGGAAGG CAGAACCTAC TTTTGGAGGT CATGACCCTC GCACAGCTGT TCAACTCCGA
    AGCCTCAGCA CAGTATTAAA ACGCCTCAAG GAAATCATGG AGGGGAAGAG CCAGGATAGT
    GACCTGAAGC AATACTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGTAATGAG
    AAATTTACCA CTTTTAGGCG CAGACACCAC TGCCGACTCT GTGGGCAGAT TTTCTGCAGT
    CGTTGCTGCA ATCAAGAAAT CCCTGGAAAA TTTATGGGTT ATACAGGGGA CCTCCGAGCC
    TGTACATACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCCATT
    GGTGAAGACT TGAATGCTCT TTCAGACTCT GCTTCCTCTG TGTCTGTACT TGATCCAAAT
    GAACCCCGTA CACCTGTGGG GAGTAGAAAA GCCAGCCGCA ACATATTTTT AGAGGATGAT
    TTTGCCTGGC AAAGTTTAAT TCATCCAGAC TCCTCAAACA CTGCTCTTTC AACAAGACTT
    GTATCTGTTC AAGAGGATGC TGGGAAATCT CCTGCTCGAA ATAGATCAGC CAGCATTACT
    AATCTGTCAC TGGATCGATC TGCGTCTCCA ATGGTACCAT CATACGAGAC GTCTGTCAGT
    CCCCAGGCTA ACCGAACGTA TGTTCGGACA GAGACCACTG AGGATGAACG CAAAATTCTT
    CTGGACAGTG TTCAGTTAAA AGACCTATGG AAGAAAATCT GCCATCACAG CAGTGGAATG
    GAGTTTCAGG ATCACCGCTA CTGGCTGAGA ACACACCCCA ACTGCATTGT AGGAAAGGAG
    TTAGTCAACT GGTTAATCCG AAATGGACAC ATTGCTACAA GGGCACAGGC TATAGCAATT
    GGACAGGCAA TGGTGGATGG ACGTTGGCTG GATTGTGTCA GTCACCACGA CCAGCTCTTC
    AGGGATGAGT ATGCGCTGTA CAGGCCGCTG CAGAGTACAG AATTTTCCGA GACCCCTTCT
    CCGGACAGTG ACTCGGTGAA CTCTGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC
    ATCAAGTTTG ATGACAGTGA CACTGAGCAG ATAGCTGAAG AAGGTGACGA TAATTTGACT
    AACTCTGCCA GTCCTAGCAA GCGCACATCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT
    GACTCAGCCG CTTCCATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG
    AAGCCCTCCA AGTACCCACA TGTGCCCCCT CACCCTGCTG ACCAAAAAGA GTATTTGATT
    TCTGACAATG GAGGACAACA GCTATCAATA AGTGACGCCT TCATCAAAGA ATCCTTATTT
    AACCGTCGAG TTGAGGAAAA ATCCAAAGAA CTGCCTTTCA CACCTTTGGG CTGGCATCAT
    AACAACCTGG AGCTGCTGAG GGAGGAGAAT GGAGAGAAAC AAGCTATGGA GAGGTTGCTT
    TCAGCTAATC ATAACCACAT GATGGCGCTG CTCCAACAGT TGCTCCATAG TGAGTCATTG
    TCAACGTCTT GGAGGGACAT CATCGTATCC CTGGTCTGCC AGGTTGTTCA GACAGTCCGA
    CCTGACGTCA AGAACCGGGA TGATGACATG GATATCCGCC AGTTTGTCCA CATCAAAAAA
    ATCCCAGGTG GAAAGAAATT TGATTCTGTG GTTGTCAATG GATTTGTTTG CACCAAGAAT
    ATTGCACATA AAAAGATGAA TTCTTCTATT AAAAACCCCA AAATTCTTCT GCTGAAATGT
    TCCATTGAAT ATCTCTACAG AGAAGAAACC AAATTTACTT GCATTGATCC TATTGTGCTT
    CAGGAAAGGG AATTCCTAAA GAATTATGTT CAGCGAATAG TTGATGTTCG ACCCACACTG
    GTTCTGGTTG AGAAAACGGT ATCTCGAATT GCACAGGATA TGTTGCTGGA ACACGGCATT
    ACTTTGGTCA TTAATGTAAA ATCACAAGTT TTGGAACGAG TTAGCCGGAT GACCCAAGGT
    GATTTGGTGA TGTCAATGGA CCAGCTGCTT ACCAAACCCC ACCTGGGCAC CTGTCACAAA
    TTTTACATGC AGATGTTTCA GTTGCCTAAT GAACAAACCA AGACACTGAT GTTTTTTGAA
    GGCTGTCCAC AGCACCTGGG CTGTACAATC AAGCTTAGAG GAGGCTCTGA TTATGAGCTG
    GCTCGAGTTA AGGAGATCTT AATATTTATG ATCTGTGTAG CTTATCATTC TCAACTAGAA
    ATCTCCTTCC TTATGGATGA ATTTGCTATG CCTCCTACAT TAACGCAAAA CCCTTCCTTC
    CATTCTCTGA TTGAAGGACA GGAGGATGAA GGGGCTTCAC AGGAGCCATT CAGCGGAAGT
    CCCCTCCCCC GGGAGCCTGA CTTCCCCCCA GACTTTCTGC CTTCTGATGA TGGCAGTTTG
    CTAGAAACAA GGATTCTGTT TGAGAAGGGT GACCAGGAGA ATAAGAGTGT GCCACAGGAT
    GTTGCCTCTT TGAAGCCTCA AGAGCATGCC CCAGCAGCTT GCCCGATGGG TGCCCCCTGT
    GCTCTCTTTC CATCAGTACC AGAGTCATTG CTTCCGCTCC ATGTGGATGA CCAACAGGAC
    GCCATAGGCA GTGAGCAGCC AGAGGCTTTG CAGCAAACAG AGGAGCTTCC GGATCCCACA
    AGCCAGATGA GAGCCTTTAG AGACCCTCTG CAGGATGACA CTGGCCTGTA TGTTACCGAG
    GAAGTTACCT CTTCTGAAGA TAAACGGAAG ACTGATTCTT TGACCTTTAA GCAAGAGTTG
    AAAGATGTGA TCCTCTGTAT CTCGCCAGTC ATCACATTCC GGGAGCCCTT CCTTTTAACT
    GACAAGGGCA TGAGATGCTC TACCCGAGAT TATTTTGCGG AGCAGGTTTA CTGGTCTCCT
    CTCCTCAATA AGGAGTTCAA GGAAATGGAG AGCAGGCGAA AGAAACAGAT GCTTAGGGAT
    CTCTCTGGCC TTCAGGGCAT GAATGGAAGT GTTCAGGCCA AGTCCATTCA AGTCCTGCCC
    TCACATGAGC TCGTGAGCAC CAGAATTGCT GAGCACCTGG GGGACAGCCA GAGCTTGGGG
    AGAATGCTAG CCGATTATCG GGCCAGAGGA GGAAGAATCC AGCAAAAAAA CTCAGACCCT
    TTTGCTTACT CAAAGGAGGC ATCAAGCACC TCAAGTGGAA AATCAGGAAG CAGAACTGAA
    GGTGATGAAG AAAAAGGGTT GATTACCAGT GATGCAGTGT GGTCGACAAA GGTGGACTGT
    CTGAACCCTG TCAACCACCA GAGGCTGTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC
    AGCAACGCTC CCAGTGCCTG TGTCAGTCCT TGGATTGTAA CAATGGAATT TTATGGAAAA
    AATGATCTTA CATTAGGAAT ATTTTTAGAG AGATACTGTT TCCGGCCTTC CTATCAGTGC
    CCTAGCATGT TCTGTGATAC CCCCATGGTG CACCACATTC GACGCTTTGT CCATGGCCAA
    GGCTGTGTGC AGATAATCCT AAAGGAGCTG GATTCTCCAG TACCTGGATA TCAGCATACA
    ATTCTCACCT ACTCCTGGTG CAGAATGTGC AAACAGGTAA CACCAGTTGT TGCTCTTTCC
    AATGAGTCTT GGTCCATGTC GTTTGCAAAA TACCTGGAAC TTAGGTTTTA TGGGCACCAG
    TACACCCGCA GAGCCAATGC TGAGCCATGT GGTCACTCCA TCCACCACGA TTATCACCAG
    TATTTCTCCT ATAACCAGAT GGTGGCGTCT TTCAGTTATT CTCCCATTCG GCTTCTTGAA
    GTGTGTGTTC CACTTCCCAA AATATTCATT AAACGTCAAG TCCCACTAAA GGTGTCCCTT
    CTTCAGGATC TGAAGGACTT CTTTCAGAAA GTTTCACAGG TATATCTTGC TGTGGATGAA
    AGACTTGCGT CTTTGAAAAC TGATACATTT AGCAAATCAA GAGAGGAAAA AATGGAAGAT
    ATCTTTGCAC AAAAAGAGAT GGAAGAAAGT GAGTTCAAGA GCTGGACTGA GAAGATGCAG
    GCGCGGCTCC TGTCTTCCTC CATGGATACC CCCCAGCAAC TGCAGTCAGT CTTCGAGTCC
    CTCATCGCCA AGAAACAAAG CCTCTGTGAG GTGCTGCAAG CTTGGAATAA CAGGCTGCAA
    GACCTTTTCC AACAAGAAAA GGGTAGGAAG CGGCCTTCAG TTCCTCCAAG TCCTGGAAGA
    CTGAGACAAG GTGAAGAAAG CAAGATAAGT GCAGTGGATG CATCTCCACG GAATGCTTCT
    CCAGGACTTC CAAATGGAGA AAAAGAGGAC CGCTTCTTAG CCACCCTTTC CAGTCAGAGC
    TCCACCAGTT CCCCCCATCT CCAGCTGCCT ACTTCTCCTG AAGGCGTGCC TGAGCAGGTC
    ATGGGAGGGC CCCCAGATCT CGATACTGCC AGCAGTTCTG AAGATGTATT TGATGGACAT
    TTGCTGGGAT CTACAGACAG CCAGGTGAAG GAAAAATCAA CCATGAAAGC CATCTTTGCA
    AATTTGCTTC CAGGAAATAG CTATAACCCT ATTCCATTTC CTTTCGATCC AGATAAACAC
    TACTTAATGT ATGAACATGA ACGAGTGCCC ATTGCAGTCT GTGAGAAGGA GCCCAGCTCC
    ATCATTGCTT TTGCTCTCAG TTGTAAAGAA TACCGGAATG CCTTAGAGGA ATTGTCCAAA
    GCCACTCAGC GGAACAGTGC TGAAGAAGGG CTTCCAACAA ACAGTACTTT AGACAGCAGA
    CCAAAGAGTA GCAGCCCAAT TAGACTACCT GAGATCAGTG GAGGACAGAC AAACCGTGCG
    GTAGAAGCAG AACCACAGCC AACCAAAAAG GCTTCTGGAA TGTTGTCCTT CTTCCGAGGG
    ACAGCAGGGA AAAGCCCTGA CCTCTCTTCC CAGAAGAGAG AGACCTTACG TGGAGCAGAT
    AGTGCTTACT ACCAAGTGGG GCAGACGGGC AAGGAGGGGA CGGAGAATCA AGGCATTGAG
    CCTCAAGATG AAGTAGATGG AGGAGATACA CAGAAGAAAC AACTTACAAA TCCTCATGTG
    GAGCTTCAAT TTTCGGATGC CAACGCCAAG TTTTACTGTC GGCTCTACTA TGCGGGAGAG
    TTTCATAAGA TGCGAGAAGT GATTCTGGGC AGCAGCGAAG AAGATTTCAT TCGTTCCCTT
    TCTCACTCTT CTCCGTGGCA GGCCCGAGGA GGCAAATCAG GAGCTGCCTT TTACGCTACT
    GAAGATGACA GATTCATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC
    TTCGCACCAC ACTACTTCAA TTACATTACA AATGCCGTTC AGCAAAAGAG GCCTACTGCA
    TTGGCCAAGA TTCTTGGAGT TTACAGAATT GGTTATAAGA ATTCTCAGAA CAACACTGAG
    AAGAAGCTAG ATCTCCTTGT CATGGAAAAT CTTTTCTACG GGAGAAAGAT GGCACAGGTT
    TTTGACTTGA AGGGTTCACT TAGGAATCGA AATGTAAAAA CCGATACCGG GAAAGAGAGC
    TGTGATGTTG TTCTTCTGGA TGAAAATCTC TTAAAGATGG TTCGAGACAA CCCTCTCTAT
    ATCCGTTCTC ATTCCAAAGC TGTGCTGAGA GCCTCGATCC GGAGCGACTC CCACTTCCTT
    TCTAGCCACC TCATTATAGA CTATTCTTTG CTAGTTGGGC GAGACGATAC CAGCAATGAG
    CTAGTAGTTG GAATTATAGA TTATATCCGA ACATTTACCT GGGACAAAAA GCTTGAGATG
    GTTGTGAAAT CAACGGGAAT TTTAGGAGGA CAAGGTAAAA TGCCGACTGT GGTCTCTCCA
    GAGTTGTACA GGACTAGATT CTGTGAAGCC ATGGACAAGT ATTTCCTGAT GGTGCCAGAC
    CACTGGACAG GCTTGGGTCT GAACTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58663
    Clone ID Related Accession (Same CDS sequence) XM_024978627.1 , XM_010802274.2
    Accession Version XM_010802274.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6330bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802274.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGAGGACAGT TTGCAACACC CCCAGGAGAA CTCAGACACC
    AGAAGGAAGG CAGAACCTAC TTTTGGAGGT CATGACCCTC GCACAGCTGT TCAACTCCGA
    AGCCTCAGCA CAGTATTAAA ACGCCTCAAG GAAATCATGG AGGGGAAGAG CCAGGATAGT
    GACCTGAAGC AATACTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGTAATGAG
    AAATTTACCA CTTTTAGGCG CAGACACCAC TGCCGACTCT GTGGGCAGAT TTTCTGCAGT
    CGTTGCTGCA ATCAAGAAAT CCCTGGAAAA TTTATGGGTT ATACAGGGGA CCTCCGAGCC
    TGTACATACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCCATT
    GGTGAAGACT TGAATGCTCT TTCAGACTCT GCTTCCTCTG TGTCTGTACT TGATCCAAAT
    GAACCCCGTA CACCTGTGGG GAGTAGAAAA GCCAGCCGCA ACATATTTTT AGAGGATGAT
    TTTGCCTGGC AAAGTTTAAT TCATCCAGAC TCCTCAAACA CTGCTCTTTC AACAAGACTT
    GTATCTGTTC AAGAGGATGC TGGGAAATCT CCTGCTCGAA ATAGATCAGC CAGCATTACT
    AATCTGTCAC TGGATCGATC TGCGTCTCCA ATGGTACCAT CATACGAGAC GTCTGTCAGT
    CCCCAGGCTA ACCGAACGTA TGTTCGGACA GAGACCACTG AGGATGAACG CAAAATTCTT
    CTGGACAGTG TTCAGTTAAA AGACCTATGG AAGAAAATCT GCCATCACAG CAGTGGAATG
    GAGTTTCAGG ATCACCGCTA CTGGCTGAGA ACACACCCCA ACTGCATTGT AGGAAAGGAG
    TTAGTCAACT GGTTAATCCG AAATGGACAC ATTGCTACAA GGGCACAGGC TATAGCAATT
    GGACAGGCAA TGGTGGATGG ACGTTGGCTG GATTGTGTCA GTCACCACGA CCAGCTCTTC
    AGGGATGAGT ATGCGCTGTA CAGGCCGCTG CAGAGTACAG AATTTTCCGA GACCCCTTCT
    CCGGACAGTG ACTCGGTGAA CTCTGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC
    ATCAAGTTTG ATGACAGTGA CACTGAGCAG ATAGCTGAAG AAGGTGACGA TAATTTGACT
    AACTCTGCCA GTCCTAGCAA GCGCACATCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT
    GACTCAGCCG CTTCCATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG
    AAGCCCTCCA AGTACCCACA TGTGCCCCCT CACCCTGCTG ACCAAAAAGA GTATTTGATT
    TCTGACAATG GAGGACAACA GCTATCAATA AGTGACGCCT TCATCAAAGA ATCCTTATTT
    AACCGTCGAG TTGAGGAAAA ATCCAAAGAA CTGCCTTTCA CACCTTTGGG CTGGCATCAT
    AACAACCTGG AGCTGCTGAG GGAGGAGAAT GGAGAGAAAC AAGCTATGGA GAGGTTGCTT
    TCAGCTAATC ATAACCACAT GATGGCGCTG CTCCAACAGT TGCTCCATAG TGAGTCATTG
    TCAACGTCTT GGAGGGACAT CATCGTATCC CTGGTCTGCC AGGTTGTTCA GACAGTCCGA
    CCTGACGTCA AGAACCGGGA TGATGACATG GATATCCGCC AGTTTGTCCA CATCAAAAAA
    ATCCCAGGTG GAAAGAAATT TGATTCTGTG GTTGTCAATG GATTTGTTTG CACCAAGAAT
    ATTGCACATA AAAAGATGAA TTCTTCTATT AAAAACCCCA AAATTCTTCT GCTGAAATGT
    TCCATTGAAT ATCTCTACAG AGAAGAAACC AAATTTACTT GCATTGATCC TATTGTGCTT
    CAGGAAAGGG AATTCCTAAA GAATTATGTT CAGCGAATAG TTGATGTTCG ACCCACACTG
    GTTCTGGTTG AGAAAACGGT ATCTCGAATT GCACAGGATA TGTTGCTGGA ACACGGCATT
    ACTTTGGTCA TTAATGTAAA ATCACAAGTT TTGGAACGAG TTAGCCGGAT GACCCAAGGT
    GATTTGGTGA TGTCAATGGA CCAGCTGCTT ACCAAACCCC ACCTGGGCAC CTGTCACAAA
    TTTTACATGC AGATGTTTCA GTTGCCTAAT GAACAAACCA AGACACTGAT GTTTTTTGAA
    GGCTGTCCAC AGCACCTGGG CTGTACAATC AAGCTTAGAG GAGGCTCTGA TTATGAGCTG
    GCTCGAGTTA AGGAGATCTT AATATTTATG ATCTGTGTAG CTTATCATTC TCAACTAGAA
    ATCTCCTTCC TTATGGATGA ATTTGCTATG CCTCCTACAT TAACGCAAAA CCCTTCCTTC
    CATTCTCTGA TTGAAGGACA GGAGGATGAA GGGGCTTCAC AGGAGCCATT CAGCGGAAGT
    CCCCTCCCCC GGGAGCCTGA CTTCCCCCCA GACTTTCTGC CTTCTGATGA TGGCAGTTTG
    CTAGAAACAA GGATTCTGTT TGAGAAGGGT GACCAGGAGA ATAAGAGTGT GCCACAGGAT
    GTTGCCTCTT TGAAGCCTCA AGAGCATGCC CCAGCAGCTT GCCCGATGGG TGCCCCCTGT
    GCTCTCTTTC CATCAGTACC AGAGTCATTG CTTCCGCTCC ATGTGGATGA CCAACAGGAC
    GCCATAGGCA GTGAGCAGCC AGAGGCTTTG CAGCAAACAG AGGAGCTTCC GGATCCCACA
    AGCCAGATGA GAGCCTTTAG AGACCCTCTG CAGGATGACA CTGGCCTGTA TGTTACCGAG
    GAAGTTACCT CTTCTGAAGA TAAACGGAAG ACTGATTCTT TGACCTTTAA GCAAGAGTTG
    AAAGATGTGA TCCTCTGTAT CTCGCCAGTC ATCACATTCC GGGAGCCCTT CCTTTTAACT
    GACAAGGGCA TGAGATGCTC TACCCGAGAT TATTTTGCGG AGCAGGTTTA CTGGTCTCCT
    CTCCTCAATA AGGAGTTCAA GGAAATGGAG AGCAGGCGAA AGAAACAGAT GCTTAGGGAT
    CTCTCTGGCC TTCAGGGCAT GAATGGAAGT GTTCAGGCCA AGTCCATTCA AGTCCTGCCC
    TCACATGAGC TCGTGAGCAC CAGAATTGCT GAGCACCTGG GGGACAGCCA GAGCTTGGGG
    AGAATGCTAG CCGATTATCG GGCCAGAGGA GGAAGAATCC AGCAAAAAAA CTCAGACCCT
    TTTGCTTACT CAAAGGAGGC ATCAAGCACC TCAAGTGGAA AATCAGGAAG CAGAACTGAA
    GGTGATGAAG AAAAAGGGTT GATTACCAGT GATGCAGTGT GGTCGACAAA GGTGGACTGT
    CTGAACCCTG TCAACCACCA GAGGCTGTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC
    AGCAACGCTC CCAGTGCCTG TGTCAGTCCT TGGATTGTAA CAATGGAATT TTATGGAAAA
    AATGATCTTA CATTAGGAAT ATTTTTAGAG AGATACTGTT TCCGGCCTTC CTATCAGTGC
    CCTAGCATGT TCTGTGATAC CCCCATGGTG CACCACATTC GACGCTTTGT CCATGGCCAA
    GGCTGTGTGC AGATAATCCT AAAGGAGCTG GATTCTCCAG TACCTGGATA TCAGCATACA
    ATTCTCACCT ACTCCTGGTG CAGAATGTGC AAACAGGTAA CACCAGTTGT TGCTCTTTCC
    AATGAGTCTT GGTCCATGTC GTTTGCAAAA TACCTGGAAC TTAGGTTTTA TGGGCACCAG
    TACACCCGCA GAGCCAATGC TGAGCCATGT GGTCACTCCA TCCACCACGA TTATCACCAG
    TATTTCTCCT ATAACCAGAT GGTGGCGTCT TTCAGTTATT CTCCCATTCG GCTTCTTGAA
    GTGTGTGTTC CACTTCCCAA AATATTCATT AAACGTCAAG TCCCACTAAA GGTGTCCCTT
    CTTCAGGATC TGAAGGACTT CTTTCAGAAA GTTTCACAGG TATATCTTGC TGTGGATGAA
    AGACTTGCGT CTTTGAAAAC TGATACATTT AGCAAATCAA GAGAGGAAAA AATGGAAGAT
    ATCTTTGCAC AAAAAGAGAT GGAAGAAAGT GAGTTCAAGA GCTGGACTGA GAAGATGCAG
    GCGCGGCTCC TGTCTTCCTC CATGGATACC CCCCAGCAAC TGCAGTCAGT CTTCGAGTCC
    CTCATCGCCA AGAAACAAAG CCTCTGTGAG GTGCTGCAAG CTTGGAATAA CAGGCTGCAA
    GACCTTTTCC AACAAGAAAA GGGTAGGAAG CGGCCTTCAG TTCCTCCAAG TCCTGGAAGA
    CTGAGACAAG GTGAAGAAAG CAAGATAAGT GCAGTGGATG CATCTCCACG GAATGCTTCT
    CCAGGACTTC CAAATGGAGA AAAAGAGGAC CGCTTCTTAG CCACCCTTTC CAGTCAGAGC
    TCCACCAGTT CCCCCCATCT CCAGCTGCCT ACTTCTCCTG AAGGCGTGCC TGAGCAGGTC
    ATGGGAGGGC CCCCAGATCT CGATACTGCC AGCAGTTCTG AAGATGTATT TGATGGACAT
    TTGCTGGGAT CTACAGACAG CCAGGTGAAG GAAAAATCAA CCATGAAAGC CATCTTTGCA
    AATTTGCTTC CAGGAAATAG CTATAACCCT ATTCCATTTC CTTTCGATCC AGATAAACAC
    TACTTAATGT ATGAACATGA ACGAGTGCCC ATTGCAGTCT GTGAGAAGGA GCCCAGCTCC
    ATCATTGCTT TTGCTCTCAG TTGTAAAGAA TACCGGAATG CCTTAGAGGA ATTGTCCAAA
    GCCACTCAGC GGAACAGTGC TGAAGAAGGG CTTCCAACAA ACAGTACTTT AGACAGCAGA
    CCAAAGAGTA GCAGCCCAAT TAGACTACCT GAGATCAGTG GAGGACAGAC AAACCGTGCG
    GTAGAAGCAG AACCACAGCC AACCAAAAAG GCTTCTGGAA TGTTGTCCTT CTTCCGAGGG
    ACAGCAGGGA AAAGCCCTGA CCTCTCTTCC CAGAAGAGAG AGACCTTACG TGGAGCAGAT
    AGTGCTTACT ACCAAGTGGG GCAGACGGGC AAGGAGGGGA CGGAGAATCA AGGCATTGAG
    CCTCAAGATG AAGTAGATGG AGGAGATACA CAGAAGAAAC AACTTACAAA TCCTCATGTG
    GAGCTTCAAT TTTCGGATGC CAACGCCAAG TTTTACTGTC GGCTCTACTA TGCGGGAGAG
    TTTCATAAGA TGCGAGAAGT GATTCTGGGC AGCAGCGAAG AAGATTTCAT TCGTTCCCTT
    TCTCACTCTT CTCCGTGGCA GGCCCGAGGA GGCAAATCAG GAGCTGCCTT TTACGCTACT
    GAAGATGACA GATTCATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC
    TTCGCACCAC ACTACTTCAA TTACATTACA AATGCCGTTC AGCAAAAGAG GCCTACTGCA
    TTGGCCAAGA TTCTTGGAGT TTACAGAATT GGTTATAAGA ATTCTCAGAA CAACACTGAG
    AAGAAGCTAG ATCTCCTTGT CATGGAAAAT CTTTTCTACG GGAGAAAGAT GGCACAGGTT
    TTTGACTTGA AGGGTTCACT TAGGAATCGA AATGTAAAAA CCGATACCGG GAAAGAGAGC
    TGTGATGTTG TTCTTCTGGA TGAAAATCTC TTAAAGATGG TTCGAGACAA CCCTCTCTAT
    ATCCGTTCTC ATTCCAAAGC TGTGCTGAGA GCCTCGATCC GGAGCGACTC CCACTTCCTT
    TCTAGCCACC TCATTATAGA CTATTCTTTG CTAGTTGGGC GAGACGATAC CAGCAATGAG
    CTAGTAGTTG GAATTATAGA TTATATCCGA ACATTTACCT GGGACAAAAA GCTTGAGATG
    GTTGTGAAAT CAACGGGAAT TTTAGGAGGA CAAGGTAAAA TGCCGACTGT GGTCTCTCCA
    GAGTTGTACA GGACTAGATT CTGTGAAGCC ATGGACAAGT ATTTCCTGAT GGTGCCAGAC
    CACTGGACAG GCTTGGGTCT GAACTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800576.1
    CDS132..6461
    Translation

    Target ORF information:

    RefSeq Version XM_010802274.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802274.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGAGGACAGT TTGCAACACC CCCAGGAGAA CTCAGACACC
    AGAAGGAAGG CAGAACCTAC TTTTGGAGGT CATGACCCTC GCACAGCTGT TCAACTCCGA
    AGCCTCAGCA CAGTATTAAA ACGCCTCAAG GAAATCATGG AGGGGAAGAG CCAGGATAGT
    GACCTGAAGC AATACTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGTAATGAG
    AAATTTACCA CTTTTAGGCG CAGACACCAC TGCCGACTCT GTGGGCAGAT TTTCTGCAGT
    CGTTGCTGCA ATCAAGAAAT CCCTGGAAAA TTTATGGGTT ATACAGGGGA CCTCCGAGCC
    TGTACATACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCCATT
    GGTGAAGACT TGAATGCTCT TTCAGACTCT GCTTCCTCTG TGTCTGTACT TGATCCAAAT
    GAACCCCGTA CACCTGTGGG GAGTAGAAAA GCCAGCCGCA ACATATTTTT AGAGGATGAT
    TTTGCCTGGC AAAGTTTAAT TCATCCAGAC TCCTCAAACA CTGCTCTTTC AACAAGACTT
    GTATCTGTTC AAGAGGATGC TGGGAAATCT CCTGCTCGAA ATAGATCAGC CAGCATTACT
    AATCTGTCAC TGGATCGATC TGCGTCTCCA ATGGTACCAT CATACGAGAC GTCTGTCAGT
    CCCCAGGCTA ACCGAACGTA TGTTCGGACA GAGACCACTG AGGATGAACG CAAAATTCTT
    CTGGACAGTG TTCAGTTAAA AGACCTATGG AAGAAAATCT GCCATCACAG CAGTGGAATG
    GAGTTTCAGG ATCACCGCTA CTGGCTGAGA ACACACCCCA ACTGCATTGT AGGAAAGGAG
    TTAGTCAACT GGTTAATCCG AAATGGACAC ATTGCTACAA GGGCACAGGC TATAGCAATT
    GGACAGGCAA TGGTGGATGG ACGTTGGCTG GATTGTGTCA GTCACCACGA CCAGCTCTTC
    AGGGATGAGT ATGCGCTGTA CAGGCCGCTG CAGAGTACAG AATTTTCCGA GACCCCTTCT
    CCGGACAGTG ACTCGGTGAA CTCTGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC
    ATCAAGTTTG ATGACAGTGA CACTGAGCAG ATAGCTGAAG AAGGTGACGA TAATTTGACT
    AACTCTGCCA GTCCTAGCAA GCGCACATCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT
    GACTCAGCCG CTTCCATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG
    AAGCCCTCCA AGTACCCACA TGTGCCCCCT CACCCTGCTG ACCAAAAAGA GTATTTGATT
    TCTGACAATG GAGGACAACA GCTATCAATA AGTGACGCCT TCATCAAAGA ATCCTTATTT
    AACCGTCGAG TTGAGGAAAA ATCCAAAGAA CTGCCTTTCA CACCTTTGGG CTGGCATCAT
    AACAACCTGG AGCTGCTGAG GGAGGAGAAT GGAGAGAAAC AAGCTATGGA GAGGTTGCTT
    TCAGCTAATC ATAACCACAT GATGGCGCTG CTCCAACAGT TGCTCCATAG TGAGTCATTG
    TCAACGTCTT GGAGGGACAT CATCGTATCC CTGGTCTGCC AGGTTGTTCA GACAGTCCGA
    CCTGACGTCA AGAACCGGGA TGATGACATG GATATCCGCC AGTTTGTCCA CATCAAAAAA
    ATCCCAGGTG GAAAGAAATT TGATTCTGTG GTTGTCAATG GATTTGTTTG CACCAAGAAT
    ATTGCACATA AAAAGATGAA TTCTTCTATT AAAAACCCCA AAATTCTTCT GCTGAAATGT
    TCCATTGAAT ATCTCTACAG AGAAGAAACC AAATTTACTT GCATTGATCC TATTGTGCTT
    CAGGAAAGGG AATTCCTAAA GAATTATGTT CAGCGAATAG TTGATGTTCG ACCCACACTG
    GTTCTGGTTG AGAAAACGGT ATCTCGAATT GCACAGGATA TGTTGCTGGA ACACGGCATT
    ACTTTGGTCA TTAATGTAAA ATCACAAGTT TTGGAACGAG TTAGCCGGAT GACCCAAGGT
    GATTTGGTGA TGTCAATGGA CCAGCTGCTT ACCAAACCCC ACCTGGGCAC CTGTCACAAA
    TTTTACATGC AGATGTTTCA GTTGCCTAAT GAACAAACCA AGACACTGAT GTTTTTTGAA
    GGCTGTCCAC AGCACCTGGG CTGTACAATC AAGCTTAGAG GAGGCTCTGA TTATGAGCTG
    GCTCGAGTTA AGGAGATCTT AATATTTATG ATCTGTGTAG CTTATCATTC TCAACTAGAA
    ATCTCCTTCC TTATGGATGA ATTTGCTATG CCTCCTACAT TAACGCAAAA CCCTTCCTTC
    CATTCTCTGA TTGAAGGACA GGAGGATGAA GGGGCTTCAC AGGAGCCATT CAGCGGAAGT
    CCCCTCCCCC GGGAGCCTGA CTTCCCCCCA GACTTTCTGC CTTCTGATGA TGGCAGTTTG
    CTAGAAACAA GGATTCTGTT TGAGAAGGGT GACCAGGAGA ATAAGAGTGT GCCACAGGAT
    GTTGCCTCTT TGAAGCCTCA AGAGCATGCC CCAGCAGCTT GCCCGATGGG TGCCCCCTGT
    GCTCTCTTTC CATCAGTACC AGAGTCATTG CTTCCGCTCC ATGTGGATGA CCAACAGGAC
    GCCATAGGCA GTGAGCAGCC AGAGGCTTTG CAGCAAACAG AGGAGCTTCC GGATCCCACA
    AGCCAGATGA GAGCCTTTAG AGACCCTCTG CAGGATGACA CTGGCCTGTA TGTTACCGAG
    GAAGTTACCT CTTCTGAAGA TAAACGGAAG ACTGATTCTT TGACCTTTAA GCAAGAGTTG
    AAAGATGTGA TCCTCTGTAT CTCGCCAGTC ATCACATTCC GGGAGCCCTT CCTTTTAACT
    GACAAGGGCA TGAGATGCTC TACCCGAGAT TATTTTGCGG AGCAGGTTTA CTGGTCTCCT
    CTCCTCAATA AGGAGTTCAA GGAAATGGAG AGCAGGCGAA AGAAACAGAT GCTTAGGGAT
    CTCTCTGGCC TTCAGGGCAT GAATGGAAGT GTTCAGGCCA AGTCCATTCA AGTCCTGCCC
    TCACATGAGC TCGTGAGCAC CAGAATTGCT GAGCACCTGG GGGACAGCCA GAGCTTGGGG
    AGAATGCTAG CCGATTATCG GGCCAGAGGA GGAAGAATCC AGCAAAAAAA CTCAGACCCT
    TTTGCTTACT CAAAGGAGGC ATCAAGCACC TCAAGTGGAA AATCAGGAAG CAGAACTGAA
    GGTGATGAAG AAAAAGGGTT GATTACCAGT GATGCAGTGT GGTCGACAAA GGTGGACTGT
    CTGAACCCTG TCAACCACCA GAGGCTGTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC
    AGCAACGCTC CCAGTGCCTG TGTCAGTCCT TGGATTGTAA CAATGGAATT TTATGGAAAA
    AATGATCTTA CATTAGGAAT ATTTTTAGAG AGATACTGTT TCCGGCCTTC CTATCAGTGC
    CCTAGCATGT TCTGTGATAC CCCCATGGTG CACCACATTC GACGCTTTGT CCATGGCCAA
    GGCTGTGTGC AGATAATCCT AAAGGAGCTG GATTCTCCAG TACCTGGATA TCAGCATACA
    ATTCTCACCT ACTCCTGGTG CAGAATGTGC AAACAGGTAA CACCAGTTGT TGCTCTTTCC
    AATGAGTCTT GGTCCATGTC GTTTGCAAAA TACCTGGAAC TTAGGTTTTA TGGGCACCAG
    TACACCCGCA GAGCCAATGC TGAGCCATGT GGTCACTCCA TCCACCACGA TTATCACCAG
    TATTTCTCCT ATAACCAGAT GGTGGCGTCT TTCAGTTATT CTCCCATTCG GCTTCTTGAA
    GTGTGTGTTC CACTTCCCAA AATATTCATT AAACGTCAAG TCCCACTAAA GGTGTCCCTT
    CTTCAGGATC TGAAGGACTT CTTTCAGAAA GTTTCACAGG TATATCTTGC TGTGGATGAA
    AGACTTGCGT CTTTGAAAAC TGATACATTT AGCAAATCAA GAGAGGAAAA AATGGAAGAT
    ATCTTTGCAC AAAAAGAGAT GGAAGAAAGT GAGTTCAAGA GCTGGACTGA GAAGATGCAG
    GCGCGGCTCC TGTCTTCCTC CATGGATACC CCCCAGCAAC TGCAGTCAGT CTTCGAGTCC
    CTCATCGCCA AGAAACAAAG CCTCTGTGAG GTGCTGCAAG CTTGGAATAA CAGGCTGCAA
    GACCTTTTCC AACAAGAAAA GGGTAGGAAG CGGCCTTCAG TTCCTCCAAG TCCTGGAAGA
    CTGAGACAAG GTGAAGAAAG CAAGATAAGT GCAGTGGATG CATCTCCACG GAATGCTTCT
    CCAGGACTTC CAAATGGAGA AAAAGAGGAC CGCTTCTTAG CCACCCTTTC CAGTCAGAGC
    TCCACCAGTT CCCCCCATCT CCAGCTGCCT ACTTCTCCTG AAGGCGTGCC TGAGCAGGTC
    ATGGGAGGGC CCCCAGATCT CGATACTGCC AGCAGTTCTG AAGATGTATT TGATGGACAT
    TTGCTGGGAT CTACAGACAG CCAGGTGAAG GAAAAATCAA CCATGAAAGC CATCTTTGCA
    AATTTGCTTC CAGGAAATAG CTATAACCCT ATTCCATTTC CTTTCGATCC AGATAAACAC
    TACTTAATGT ATGAACATGA ACGAGTGCCC ATTGCAGTCT GTGAGAAGGA GCCCAGCTCC
    ATCATTGCTT TTGCTCTCAG TTGTAAAGAA TACCGGAATG CCTTAGAGGA ATTGTCCAAA
    GCCACTCAGC GGAACAGTGC TGAAGAAGGG CTTCCAACAA ACAGTACTTT AGACAGCAGA
    CCAAAGAGTA GCAGCCCAAT TAGACTACCT GAGATCAGTG GAGGACAGAC AAACCGTGCG
    GTAGAAGCAG AACCACAGCC AACCAAAAAG GCTTCTGGAA TGTTGTCCTT CTTCCGAGGG
    ACAGCAGGGA AAAGCCCTGA CCTCTCTTCC CAGAAGAGAG AGACCTTACG TGGAGCAGAT
    AGTGCTTACT ACCAAGTGGG GCAGACGGGC AAGGAGGGGA CGGAGAATCA AGGCATTGAG
    CCTCAAGATG AAGTAGATGG AGGAGATACA CAGAAGAAAC AACTTACAAA TCCTCATGTG
    GAGCTTCAAT TTTCGGATGC CAACGCCAAG TTTTACTGTC GGCTCTACTA TGCGGGAGAG
    TTTCATAAGA TGCGAGAAGT GATTCTGGGC AGCAGCGAAG AAGATTTCAT TCGTTCCCTT
    TCTCACTCTT CTCCGTGGCA GGCCCGAGGA GGCAAATCAG GAGCTGCCTT TTACGCTACT
    GAAGATGACA GATTCATTTT GAAGCAAATG CCTCGTCTGG AAGTCCAGTC TTTCCTTGAC
    TTCGCACCAC ACTACTTCAA TTACATTACA AATGCCGTTC AGCAAAAGAG GCCTACTGCA
    TTGGCCAAGA TTCTTGGAGT TTACAGAATT GGTTATAAGA ATTCTCAGAA CAACACTGAG
    AAGAAGCTAG ATCTCCTTGT CATGGAAAAT CTTTTCTACG GGAGAAAGAT GGCACAGGTT
    TTTGACTTGA AGGGTTCACT TAGGAATCGA AATGTAAAAA CCGATACCGG GAAAGAGAGC
    TGTGATGTTG TTCTTCTGGA TGAAAATCTC TTAAAGATGG TTCGAGACAA CCCTCTCTAT
    ATCCGTTCTC ATTCCAAAGC TGTGCTGAGA GCCTCGATCC GGAGCGACTC CCACTTCCTT
    TCTAGCCACC TCATTATAGA CTATTCTTTG CTAGTTGGGC GAGACGATAC CAGCAATGAG
    CTAGTAGTTG GAATTATAGA TTATATCCGA ACATTTACCT GGGACAAAAA GCTTGAGATG
    GTTGTGAAAT CAACGGGAAT TTTAGGAGGA CAAGGTAAAA TGCCGACTGT GGTCTCTCCA
    GAGTTGTACA GGACTAGATT CTGTGAAGCC ATGGACAAGT ATTTCCTGAT GGTGCCAGAC
    CACTGGACAG GCTTGGGTCT GAACTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58664
    Clone ID Related Accession (Same CDS sequence) XM_024978643.1 , XM_010802275.2
    Accession Version XM_024978643.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6168bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTGGAAC GAGTTAGCCG GATGACCCAA
    GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC
    AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT
    GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG
    CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA
    GAAATCTCCT TCCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC
    TTCCATTCTC TGATTGAAGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA
    AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT
    TTGCTAGAAA CAAGGATTCT GTTTGAGAAG GGTGACCAGG AGAATAAGAG TGTGCCACAG
    GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC
    TGTGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG
    GACGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA CAGAGGAGCT TCCGGATCCC
    ACAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGCCT GTATGTTACC
    GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG
    TTGAAAGATG TGATCCTCTG TATCTCGCCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA
    ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CGGAGCAGGT TTACTGGTCT
    CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GAAAGAAACA GATGCTTAGG
    GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG
    CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG
    GGGAGAATGC TAGCCGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC
    CCTTTTGCTT ACTCAAAGGA GGCATCAAGC ACCTCAAGTG GAAAATCAGG AAGCAGAACT
    GAAGGTGATG AAGAAAAAGG GTTGATTACC AGTGATGCAG TGTGGTCGAC AAAGGTGGAC
    TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG
    TCCAGCAACG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA
    AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG
    TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC
    CAAGGCTGTG TGCAGATAAT CCTAAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT
    ACAATTCTCA CCTACTCCTG GTGCAGAATG TGCAAACAGG TAACACCAGT TGTTGCTCTT
    TCCAATGAGT CTTGGTCCAT GTCGTTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC
    CAGTACACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA CGATTATCAC
    CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT
    GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCACT AAAGGTGTCC
    CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG AAAGTTTCAC AGGTATATCT TGCTGTGGAT
    GAAAGACTTG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA
    GATATCTTTG CACAAAAAGA GATGGAAGAA AGTGAGTTCA AGAGCTGGAC TGAGAAGATG
    CAGGCGCGGC TCCTGTCTTC CTCCATGGAT ACCCCCCAGC AACTGCAGTC AGTCTTCGAG
    TCCCTCATCG CCAAGAAACA AAGCCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG
    CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA
    AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT
    TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT TTCCAGTCAG
    AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGCGT GCCTGAGCAG
    GTCATGGGAG GGCCCCCAGA TCTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA
    CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT
    GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTCGA TCCAGATAAA
    CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC
    TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC
    AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC
    AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT
    GCGGTAGAAG CAGAACCACA GCCAAATGAA GTAGATGGAG GAGATACACA GAAGAAACAA
    CTTACAAATC CTCATGTGGA GCTTCAATTT TCGGATGCCA ACGCCAAGTT TTACTGTCGG
    CTCTACTATG CGGGAGAGTT TCATAAGATG CGAGAAGTGA TTCTGGGCAG CAGCGAAGAA
    GATTTCATTC GTTCCCTTTC TCACTCTTCT CCGTGGCAGG CCCGAGGAGG CAAATCAGGA
    GCTGCCTTTT ACGCTACTGA AGATGACAGA TTCATTTTGA AGCAAATGCC TCGTCTGGAA
    GTCCAGTCTT TCCTTGACTT CGCACCACAC TACTTCAATT ACATTACAAA TGCCGTTCAG
    CAAAAGAGGC CTACTGCATT GGCCAAGATT CTTGGAGTTT ACAGAATTGG TTATAAGAAT
    TCTCAGAACA ACACTGAGAA GAAGCTAGAT CTCCTTGTCA TGGAAAATCT TTTCTACGGG
    AGAAAGATGG CACAGGTTTT TGACTTGAAG GGTTCACTTA GGAATCGAAA TGTAAAAACC
    GATACCGGGA AAGAGAGCTG TGATGTTGTT CTTCTGGATG AAAATCTCTT AAAGATGGTT
    CGAGACAACC CTCTCTATAT CCGTTCTCAT TCCAAAGCTG TGCTGAGAGC CTCGATCCGG
    AGCGACTCCC ACTTCCTTTC TAGCCACCTC ATTATAGACT ATTCTTTGCT AGTTGGGCGA
    GACGATACCA GCAATGAGCT AGTAGTTGGA ATTATAGATT ATATCCGAAC ATTTACCTGG
    GACAAAAAGC TTGAGATGGT TGTGAAATCA ACGGGAATTT TAGGAGGACA AGGTAAAATG
    CCGACTGTGG TCTCTCCAGA GTTGTACAGG ACTAGATTCT GTGAAGCCAT GGACAAGTAT
    TTCCTGATGG TGCCAGACCA CTGGACAGGC TTGGGTCTGA ACTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834411.1
    CDS155..6322
    Translation

    Target ORF information:

    RefSeq Version XM_024978643.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978643.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTGGAAC GAGTTAGCCG GATGACCCAA
    GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC
    AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT
    GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG
    CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA
    GAAATCTCCT TCCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC
    TTCCATTCTC TGATTGAAGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA
    AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT
    TTGCTAGAAA CAAGGATTCT GTTTGAGAAG GGTGACCAGG AGAATAAGAG TGTGCCACAG
    GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC
    TGTGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG
    GACGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA CAGAGGAGCT TCCGGATCCC
    ACAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGCCT GTATGTTACC
    GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG
    TTGAAAGATG TGATCCTCTG TATCTCGCCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA
    ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CGGAGCAGGT TTACTGGTCT
    CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GAAAGAAACA GATGCTTAGG
    GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG
    CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG
    GGGAGAATGC TAGCCGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC
    CCTTTTGCTT ACTCAAAGGA GGCATCAAGC ACCTCAAGTG GAAAATCAGG AAGCAGAACT
    GAAGGTGATG AAGAAAAAGG GTTGATTACC AGTGATGCAG TGTGGTCGAC AAAGGTGGAC
    TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG
    TCCAGCAACG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA
    AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG
    TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC
    CAAGGCTGTG TGCAGATAAT CCTAAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT
    ACAATTCTCA CCTACTCCTG GTGCAGAATG TGCAAACAGG TAACACCAGT TGTTGCTCTT
    TCCAATGAGT CTTGGTCCAT GTCGTTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC
    CAGTACACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA CGATTATCAC
    CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT
    GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCACT AAAGGTGTCC
    CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG AAAGTTTCAC AGGTATATCT TGCTGTGGAT
    GAAAGACTTG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA
    GATATCTTTG CACAAAAAGA GATGGAAGAA AGTGAGTTCA AGAGCTGGAC TGAGAAGATG
    CAGGCGCGGC TCCTGTCTTC CTCCATGGAT ACCCCCCAGC AACTGCAGTC AGTCTTCGAG
    TCCCTCATCG CCAAGAAACA AAGCCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG
    CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA
    AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT
    TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT TTCCAGTCAG
    AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGCGT GCCTGAGCAG
    GTCATGGGAG GGCCCCCAGA TCTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA
    CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT
    GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTCGA TCCAGATAAA
    CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC
    TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC
    AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC
    AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT
    GCGGTAGAAG CAGAACCACA GCCAAATGAA GTAGATGGAG GAGATACACA GAAGAAACAA
    CTTACAAATC CTCATGTGGA GCTTCAATTT TCGGATGCCA ACGCCAAGTT TTACTGTCGG
    CTCTACTATG CGGGAGAGTT TCATAAGATG CGAGAAGTGA TTCTGGGCAG CAGCGAAGAA
    GATTTCATTC GTTCCCTTTC TCACTCTTCT CCGTGGCAGG CCCGAGGAGG CAAATCAGGA
    GCTGCCTTTT ACGCTACTGA AGATGACAGA TTCATTTTGA AGCAAATGCC TCGTCTGGAA
    GTCCAGTCTT TCCTTGACTT CGCACCACAC TACTTCAATT ACATTACAAA TGCCGTTCAG
    CAAAAGAGGC CTACTGCATT GGCCAAGATT CTTGGAGTTT ACAGAATTGG TTATAAGAAT
    TCTCAGAACA ACACTGAGAA GAAGCTAGAT CTCCTTGTCA TGGAAAATCT TTTCTACGGG
    AGAAAGATGG CACAGGTTTT TGACTTGAAG GGTTCACTTA GGAATCGAAA TGTAAAAACC
    GATACCGGGA AAGAGAGCTG TGATGTTGTT CTTCTGGATG AAAATCTCTT AAAGATGGTT
    CGAGACAACC CTCTCTATAT CCGTTCTCAT TCCAAAGCTG TGCTGAGAGC CTCGATCCGG
    AGCGACTCCC ACTTCCTTTC TAGCCACCTC ATTATAGACT ATTCTTTGCT AGTTGGGCGA
    GACGATACCA GCAATGAGCT AGTAGTTGGA ATTATAGATT ATATCCGAAC ATTTACCTGG
    GACAAAAAGC TTGAGATGGT TGTGAAATCA ACGGGAATTT TAGGAGGACA AGGTAAAATG
    CCGACTGTGG TCTCTCCAGA GTTGTACAGG ACTAGATTCT GTGAAGCCAT GGACAAGTAT
    TTCCTGATGG TGCCAGACCA CTGGACAGGC TTGGGTCTGA ACTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58664
    Clone ID Related Accession (Same CDS sequence) XM_024978643.1 , XM_010802275.2
    Accession Version XM_010802275.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6168bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802275.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTGGAAC GAGTTAGCCG GATGACCCAA
    GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC
    AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT
    GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG
    CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA
    GAAATCTCCT TCCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC
    TTCCATTCTC TGATTGAAGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA
    AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT
    TTGCTAGAAA CAAGGATTCT GTTTGAGAAG GGTGACCAGG AGAATAAGAG TGTGCCACAG
    GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC
    TGTGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG
    GACGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA CAGAGGAGCT TCCGGATCCC
    ACAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGCCT GTATGTTACC
    GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG
    TTGAAAGATG TGATCCTCTG TATCTCGCCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA
    ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CGGAGCAGGT TTACTGGTCT
    CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GAAAGAAACA GATGCTTAGG
    GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG
    CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG
    GGGAGAATGC TAGCCGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC
    CCTTTTGCTT ACTCAAAGGA GGCATCAAGC ACCTCAAGTG GAAAATCAGG AAGCAGAACT
    GAAGGTGATG AAGAAAAAGG GTTGATTACC AGTGATGCAG TGTGGTCGAC AAAGGTGGAC
    TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG
    TCCAGCAACG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA
    AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG
    TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC
    CAAGGCTGTG TGCAGATAAT CCTAAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT
    ACAATTCTCA CCTACTCCTG GTGCAGAATG TGCAAACAGG TAACACCAGT TGTTGCTCTT
    TCCAATGAGT CTTGGTCCAT GTCGTTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC
    CAGTACACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA CGATTATCAC
    CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT
    GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCACT AAAGGTGTCC
    CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG AAAGTTTCAC AGGTATATCT TGCTGTGGAT
    GAAAGACTTG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA
    GATATCTTTG CACAAAAAGA GATGGAAGAA AGTGAGTTCA AGAGCTGGAC TGAGAAGATG
    CAGGCGCGGC TCCTGTCTTC CTCCATGGAT ACCCCCCAGC AACTGCAGTC AGTCTTCGAG
    TCCCTCATCG CCAAGAAACA AAGCCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG
    CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA
    AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT
    TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT TTCCAGTCAG
    AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGCGT GCCTGAGCAG
    GTCATGGGAG GGCCCCCAGA TCTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA
    CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT
    GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTCGA TCCAGATAAA
    CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC
    TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC
    AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC
    AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT
    GCGGTAGAAG CAGAACCACA GCCAAATGAA GTAGATGGAG GAGATACACA GAAGAAACAA
    CTTACAAATC CTCATGTGGA GCTTCAATTT TCGGATGCCA ACGCCAAGTT TTACTGTCGG
    CTCTACTATG CGGGAGAGTT TCATAAGATG CGAGAAGTGA TTCTGGGCAG CAGCGAAGAA
    GATTTCATTC GTTCCCTTTC TCACTCTTCT CCGTGGCAGG CCCGAGGAGG CAAATCAGGA
    GCTGCCTTTT ACGCTACTGA AGATGACAGA TTCATTTTGA AGCAAATGCC TCGTCTGGAA
    GTCCAGTCTT TCCTTGACTT CGCACCACAC TACTTCAATT ACATTACAAA TGCCGTTCAG
    CAAAAGAGGC CTACTGCATT GGCCAAGATT CTTGGAGTTT ACAGAATTGG TTATAAGAAT
    TCTCAGAACA ACACTGAGAA GAAGCTAGAT CTCCTTGTCA TGGAAAATCT TTTCTACGGG
    AGAAAGATGG CACAGGTTTT TGACTTGAAG GGTTCACTTA GGAATCGAAA TGTAAAAACC
    GATACCGGGA AAGAGAGCTG TGATGTTGTT CTTCTGGATG AAAATCTCTT AAAGATGGTT
    CGAGACAACC CTCTCTATAT CCGTTCTCAT TCCAAAGCTG TGCTGAGAGC CTCGATCCGG
    AGCGACTCCC ACTTCCTTTC TAGCCACCTC ATTATAGACT ATTCTTTGCT AGTTGGGCGA
    GACGATACCA GCAATGAGCT AGTAGTTGGA ATTATAGATT ATATCCGAAC ATTTACCTGG
    GACAAAAAGC TTGAGATGGT TGTGAAATCA ACGGGAATTT TAGGAGGACA AGGTAAAATG
    CCGACTGTGG TCTCTCCAGA GTTGTACAGG ACTAGATTCT GTGAAGCCAT GGACAAGTAT
    TTCCTGATGG TGCCAGACCA CTGGACAGGC TTGGGTCTGA ACTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800577.1
    CDS132..6299
    Translation

    Target ORF information:

    RefSeq Version XM_010802275.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802275.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAACTCTG CCAGTCCTAG CAAGCGCACA TCAGTCAGCA GTTTCCAGTC CACAGTGGAC
    AGTGACTCAG CCGCTTCCAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC
    AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG
    ATTTCTGACA ATGGAGGACA ACAGCTATCA ATAAGTGACG CCTTCATCAA AGAATCCTTA
    TTTAACCGTC GAGTTGAGGA AAAATCCAAA GAACTGCCTT TCACACCTTT GGGCTGGCAT
    CATAACAACC TGGAGCTGCT GAGGGAGGAG AATGGAGAGA AACAAGCTAT GGAGAGGTTG
    CTTTCAGCTA ATCATAACCA CATGATGGCG CTGCTCCAAC AGTTGCTCCA TAGTGAGTCA
    TTGTCAACGT CTTGGAGGGA CATCATCGTA TCCCTGGTCT GCCAGGTTGT TCAGACAGTC
    CGACCTGACG TCAAGAACCG GGATGATGAC ATGGATATCC GCCAGTTTGT CCACATCAAA
    AAAATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGATTTGT TTGCACCAAG
    AATATTGCAC ATAAAAAGAT GAATTCTTCT ATTAAAAACC CCAAAATTCT TCTGCTGAAA
    TGTTCCATTG AATATCTCTA CAGAGAAGAA ACCAAATTTA CTTGCATTGA TCCTATTGTG
    CTTCAGGAAA GGGAATTCCT AAAGAATTAT GTTCAGCGAA TAGTTGATGT TCGACCCACA
    CTGGTTCTGG TTGAGAAAAC GGTATCTCGA ATTGCACAGG ATATGTTGCT GGAACACGGC
    ATTACTTTGG TCATTAATGT AAAATCACAA GTTTTGGAAC GAGTTAGCCG GATGACCCAA
    GGTGATTTGG TGATGTCAAT GGACCAGCTG CTTACCAAAC CCCACCTGGG CACCTGTCAC
    AAATTTTACA TGCAGATGTT TCAGTTGCCT AATGAACAAA CCAAGACACT GATGTTTTTT
    GAAGGCTGTC CACAGCACCT GGGCTGTACA ATCAAGCTTA GAGGAGGCTC TGATTATGAG
    CTGGCTCGAG TTAAGGAGAT CTTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAACTA
    GAAATCTCCT TCCTTATGGA TGAATTTGCT ATGCCTCCTA CATTAACGCA AAACCCTTCC
    TTCCATTCTC TGATTGAAGG ACAGGAGGAT GAAGGGGCTT CACAGGAGCC ATTCAGCGGA
    AGTCCCCTCC CCCGGGAGCC TGACTTCCCC CCAGACTTTC TGCCTTCTGA TGATGGCAGT
    TTGCTAGAAA CAAGGATTCT GTTTGAGAAG GGTGACCAGG AGAATAAGAG TGTGCCACAG
    GATGTTGCCT CTTTGAAGCC TCAAGAGCAT GCCCCAGCAG CTTGCCCGAT GGGTGCCCCC
    TGTGCTCTCT TTCCATCAGT ACCAGAGTCA TTGCTTCCGC TCCATGTGGA TGACCAACAG
    GACGCCATAG GCAGTGAGCA GCCAGAGGCT TTGCAGCAAA CAGAGGAGCT TCCGGATCCC
    ACAAGCCAGA TGAGAGCCTT TAGAGACCCT CTGCAGGATG ACACTGGCCT GTATGTTACC
    GAGGAAGTTA CCTCTTCTGA AGATAAACGG AAGACTGATT CTTTGACCTT TAAGCAAGAG
    TTGAAAGATG TGATCCTCTG TATCTCGCCA GTCATCACAT TCCGGGAGCC CTTCCTTTTA
    ACTGACAAGG GCATGAGATG CTCTACCCGA GATTATTTTG CGGAGCAGGT TTACTGGTCT
    CCTCTCCTCA ATAAGGAGTT CAAGGAAATG GAGAGCAGGC GAAAGAAACA GATGCTTAGG
    GATCTCTCTG GCCTTCAGGG CATGAATGGA AGTGTTCAGG CCAAGTCCAT TCAAGTCCTG
    CCCTCACATG AGCTCGTGAG CACCAGAATT GCTGAGCACC TGGGGGACAG CCAGAGCTTG
    GGGAGAATGC TAGCCGATTA TCGGGCCAGA GGAGGAAGAA TCCAGCAAAA AAACTCAGAC
    CCTTTTGCTT ACTCAAAGGA GGCATCAAGC ACCTCAAGTG GAAAATCAGG AAGCAGAACT
    GAAGGTGATG AAGAAAAAGG GTTGATTACC AGTGATGCAG TGTGGTCGAC AAAGGTGGAC
    TGTCTGAACC CTGTCAACCA CCAGAGGCTG TGTGTGCTCT TCAGCAGCTC TTCTGCCCAG
    TCCAGCAACG CTCCCAGTGC CTGTGTCAGT CCTTGGATTG TAACAATGGA ATTTTATGGA
    AAAAATGATC TTACATTAGG AATATTTTTA GAGAGATACT GTTTCCGGCC TTCCTATCAG
    TGCCCTAGCA TGTTCTGTGA TACCCCCATG GTGCACCACA TTCGACGCTT TGTCCATGGC
    CAAGGCTGTG TGCAGATAAT CCTAAAGGAG CTGGATTCTC CAGTACCTGG ATATCAGCAT
    ACAATTCTCA CCTACTCCTG GTGCAGAATG TGCAAACAGG TAACACCAGT TGTTGCTCTT
    TCCAATGAGT CTTGGTCCAT GTCGTTTGCA AAATACCTGG AACTTAGGTT TTATGGGCAC
    CAGTACACCC GCAGAGCCAA TGCTGAGCCA TGTGGTCACT CCATCCACCA CGATTATCAC
    CAGTATTTCT CCTATAACCA GATGGTGGCG TCTTTCAGTT ATTCTCCCAT TCGGCTTCTT
    GAAGTGTGTG TTCCACTTCC CAAAATATTC ATTAAACGTC AAGTCCCACT AAAGGTGTCC
    CTTCTTCAGG ATCTGAAGGA CTTCTTTCAG AAAGTTTCAC AGGTATATCT TGCTGTGGAT
    GAAAGACTTG CGTCTTTGAA AACTGATACA TTTAGCAAAT CAAGAGAGGA AAAAATGGAA
    GATATCTTTG CACAAAAAGA GATGGAAGAA AGTGAGTTCA AGAGCTGGAC TGAGAAGATG
    CAGGCGCGGC TCCTGTCTTC CTCCATGGAT ACCCCCCAGC AACTGCAGTC AGTCTTCGAG
    TCCCTCATCG CCAAGAAACA AAGCCTCTGT GAGGTGCTGC AAGCTTGGAA TAACAGGCTG
    CAAGACCTTT TCCAACAAGA AAAGGGTAGG AAGCGGCCTT CAGTTCCTCC AAGTCCTGGA
    AGACTGAGAC AAGGTGAAGA AAGCAAGATA AGTGCAGTGG ATGCATCTCC ACGGAATGCT
    TCTCCAGGAC TTCCAAATGG AGAAAAAGAG GACCGCTTCT TAGCCACCCT TTCCAGTCAG
    AGCTCCACCA GTTCCCCCCA TCTCCAGCTG CCTACTTCTC CTGAAGGCGT GCCTGAGCAG
    GTCATGGGAG GGCCCCCAGA TCTCGATACT GCCAGCAGTT CTGAAGATGT ATTTGATGGA
    CATTTGCTGG GATCTACAGA CAGCCAGGTG AAGGAAAAAT CAACCATGAA AGCCATCTTT
    GCAAATTTGC TTCCAGGAAA TAGCTATAAC CCTATTCCAT TTCCTTTCGA TCCAGATAAA
    CACTACTTAA TGTATGAACA TGAACGAGTG CCCATTGCAG TCTGTGAGAA GGAGCCCAGC
    TCCATCATTG CTTTTGCTCT CAGTTGTAAA GAATACCGGA ATGCCTTAGA GGAATTGTCC
    AAAGCCACTC AGCGGAACAG TGCTGAAGAA GGGCTTCCAA CAAACAGTAC TTTAGACAGC
    AGACCAAAGA GTAGCAGCCC AATTAGACTA CCTGAGATCA GTGGAGGACA GACAAACCGT
    GCGGTAGAAG CAGAACCACA GCCAAATGAA GTAGATGGAG GAGATACACA GAAGAAACAA
    CTTACAAATC CTCATGTGGA GCTTCAATTT TCGGATGCCA ACGCCAAGTT TTACTGTCGG
    CTCTACTATG CGGGAGAGTT TCATAAGATG CGAGAAGTGA TTCTGGGCAG CAGCGAAGAA
    GATTTCATTC GTTCCCTTTC TCACTCTTCT CCGTGGCAGG CCCGAGGAGG CAAATCAGGA
    GCTGCCTTTT ACGCTACTGA AGATGACAGA TTCATTTTGA AGCAAATGCC TCGTCTGGAA
    GTCCAGTCTT TCCTTGACTT CGCACCACAC TACTTCAATT ACATTACAAA TGCCGTTCAG
    CAAAAGAGGC CTACTGCATT GGCCAAGATT CTTGGAGTTT ACAGAATTGG TTATAAGAAT
    TCTCAGAACA ACACTGAGAA GAAGCTAGAT CTCCTTGTCA TGGAAAATCT TTTCTACGGG
    AGAAAGATGG CACAGGTTTT TGACTTGAAG GGTTCACTTA GGAATCGAAA TGTAAAAACC
    GATACCGGGA AAGAGAGCTG TGATGTTGTT CTTCTGGATG AAAATCTCTT AAAGATGGTT
    CGAGACAACC CTCTCTATAT CCGTTCTCAT TCCAAAGCTG TGCTGAGAGC CTCGATCCGG
    AGCGACTCCC ACTTCCTTTC TAGCCACCTC ATTATAGACT ATTCTTTGCT AGTTGGGCGA
    GACGATACCA GCAATGAGCT AGTAGTTGGA ATTATAGATT ATATCCGAAC ATTTACCTGG
    GACAAAAAGC TTGAGATGGT TGTGAAATCA ACGGGAATTT TAGGAGGACA AGGTAAAATG
    CCGACTGTGG TCTCTCCAGA GTTGTACAGG ACTAGATTCT GTGAAGCCAT GGACAAGTAT
    TTCCTGATGG TGCCAGACCA CTGGACAGGC TTGGGTCTGA ACTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58665
    Clone ID Related Accession (Same CDS sequence) XM_010802276.2 , XM_024978647.1
    Accession Version XM_010802276.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802276.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAAGTATT TGATTTCTGA CAATGGAGGA CAACAGCTAT CAATAAGTGA CGCCTTCATC
    AAAGAATCCT TATTTAACCG TCGAGTTGAG GAAAAATCCA AAGAACTGCC TTTCACACCT
    TTGGGCTGGC ATCATAACAA CCTGGAGCTG CTGAGGGAGG AGAATGGAGA GAAACAAGCT
    ATGGAGAGGT TGCTTTCAGC TAATCATAAC CACATGATGG CGCTGCTCCA ACAGTTGCTC
    CATAGTGAGT CATTGTCAAC GTCTTGGAGG GACATCATCG TATCCCTGGT CTGCCAGGTT
    GTTCAGACAG TCCGACCTGA CGTCAAGAAC CGGGATGATG ACATGGATAT CCGCCAGTTT
    GTCCACATCA AAAAAATCCC AGGTGGAAAG AAATTTGATT CTGTGGTTGT CAATGGATTT
    GTTTGCACCA AGAATATTGC ACATAAAAAG ATGAATTCTT CTATTAAAAA CCCCAAAATT
    CTTCTGCTGA AATGTTCCAT TGAATATCTC TACAGAGAAG AAACCAAATT TACTTGCATT
    GATCCTATTG TGCTTCAGGA AAGGGAATTC CTAAAGAATT ATGTTCAGCG AATAGTTGAT
    GTTCGACCCA CACTGGTTCT GGTTGAGAAA ACGGTATCTC GAATTGCACA GGATATGTTG
    CTGGAACACG GCATTACTTT GGTCATTAAT GTAAAATCAC AAGTTTTGGA ACGAGTTAGC
    CGGATGACCC AAGGTGATTT GGTGATGTCA ATGGACCAGC TGCTTACCAA ACCCCACCTG
    GGCACCTGTC ACAAATTTTA CATGCAGATG TTTCAGTTGC CTAATGAACA AACCAAGACA
    CTGATGTTTT TTGAAGGCTG TCCACAGCAC CTGGGCTGTA CAATCAAGCT TAGAGGAGGC
    TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCTTAATAT TTATGATCTG TGTAGCTTAT
    CATTCTCAAC TAGAAATCTC CTTCCTTATG GATGAATTTG CTATGCCTCC TACATTAACG
    CAAAACCCTT CCTTCCATTC TCTGATTGAA GGACAGGAGG ATGAAGGGGC TTCACAGGAG
    CCATTCAGCG GAAGTCCCCT CCCCCGGGAG CCTGACTTCC CCCCAGACTT TCTGCCTTCT
    GATGATGGCA GTTTGCTAGA AACAAGGATT CTGTTTGAGA AGGGTGACCA GGAGAATAAG
    AGTGTGCCAC AGGATGTTGC CTCTTTGAAG CCTCAAGAGC ATGCCCCAGC AGCTTGCCCG
    ATGGGTGCCC CCTGTGCTCT CTTTCCATCA GTACCAGAGT CATTGCTTCC GCTCCATGTG
    GATGACCAAC AGGACGCCAT AGGCAGTGAG CAGCCAGAGG CTTTGCAGCA AACAGAGGAG
    CTTCCGGATC CCACAAGCCA GATGAGAGCC TTTAGAGACC CTCTGCAGGA TGACACTGGC
    CTGTATGTTA CCGAGGAAGT TACCTCTTCT GAAGATAAAC GGAAGACTGA TTCTTTGACC
    TTTAAGCAAG AGTTGAAAGA TGTGATCCTC TGTATCTCGC CAGTCATCAC ATTCCGGGAG
    CCCTTCCTTT TAACTGACAA GGGCATGAGA TGCTCTACCC GAGATTATTT TGCGGAGCAG
    GTTTACTGGT CTCCTCTCCT CAATAAGGAG TTCAAGGAAA TGGAGAGCAG GCGAAAGAAA
    CAGATGCTTA GGGATCTCTC TGGCCTTCAG GGCATGAATG GAAGTGTTCA GGCCAAGTCC
    ATTCAAGTCC TGCCCTCACA TGAGCTCGTG AGCACCAGAA TTGCTGAGCA CCTGGGGGAC
    AGCCAGAGCT TGGGGAGAAT GCTAGCCGAT TATCGGGCCA GAGGAGGAAG AATCCAGCAA
    AAAAACTCAG ACCCTTTTGC TTACTCAAAG GAGGCATCAA GCACCTCAAG TGGAAAATCA
    GGAAGCAGAA CTGAAGGTGA TGAAGAAAAA GGGTTGATTA CCAGTGATGC AGTGTGGTCG
    ACAAAGGTGG ACTGTCTGAA CCCTGTCAAC CACCAGAGGC TGTGTGTGCT CTTCAGCAGC
    TCTTCTGCCC AGTCCAGCAA CGCTCCCAGT GCCTGTGTCA GTCCTTGGAT TGTAACAATG
    GAATTTTATG GAAAAAATGA TCTTACATTA GGAATATTTT TAGAGAGATA CTGTTTCCGG
    CCTTCCTATC AGTGCCCTAG CATGTTCTGT GATACCCCCA TGGTGCACCA CATTCGACGC
    TTTGTCCATG GCCAAGGCTG TGTGCAGATA ATCCTAAAGG AGCTGGATTC TCCAGTACCT
    GGATATCAGC ATACAATTCT CACCTACTCC TGGTGCAGAA TGTGCAAACA GGTAACACCA
    GTTGTTGCTC TTTCCAATGA GTCTTGGTCC ATGTCGTTTG CAAAATACCT GGAACTTAGG
    TTTTATGGGC ACCAGTACAC CCGCAGAGCC AATGCTGAGC CATGTGGTCA CTCCATCCAC
    CACGATTATC ACCAGTATTT CTCCTATAAC CAGATGGTGG CGTCTTTCAG TTATTCTCCC
    ATTCGGCTTC TTGAAGTGTG TGTTCCACTT CCCAAAATAT TCATTAAACG TCAAGTCCCA
    CTAAAGGTGT CCCTTCTTCA GGATCTGAAG GACTTCTTTC AGAAAGTTTC ACAGGTATAT
    CTTGCTGTGG ATGAAAGACT TGCGTCTTTG AAAACTGATA CATTTAGCAA ATCAAGAGAG
    GAAAAAATGG AAGATATCTT TGCACAAAAA GAGATGGAAG AAAGTGAGTT CAAGAGCTGG
    ACTGAGAAGA TGCAGGCGCG GCTCCTGTCT TCCTCCATGG ATACCCCCCA GCAACTGCAG
    TCAGTCTTCG AGTCCCTCAT CGCCAAGAAA CAAAGCCTCT GTGAGGTGCT GCAAGCTTGG
    AATAACAGGC TGCAAGACCT TTTCCAACAA GAAAAGGGTA GGAAGCGGCC TTCAGTTCCT
    CCAAGTCCTG GAAGACTGAG ACAAGGTGAA GAAAGCAAGA TAAGTGCAGT GGATGCATCT
    CCACGGAATG CTTCTCCAGG ACTTCCAAAT GGAGAAAAAG AGGACCGCTT CTTAGCCACC
    CTTTCCAGTC AGAGCTCCAC CAGTTCCCCC CATCTCCAGC TGCCTACTTC TCCTGAAGGC
    GTGCCTGAGC AGGTCATGGG AGGGCCCCCA GATCTCGATA CTGCCAGCAG TTCTGAAGAT
    GTATTTGATG GACATTTGCT GGGATCTACA GACAGCCAGG TGAAGGAAAA ATCAACCATG
    AAAGCCATCT TTGCAAATTT GCTTCCAGGA AATAGCTATA ACCCTATTCC ATTTCCTTTC
    GATCCAGATA AACACTACTT AATGTATGAA CATGAACGAG TGCCCATTGC AGTCTGTGAG
    AAGGAGCCCA GCTCCATCAT TGCTTTTGCT CTCAGTTGTA AAGAATACCG GAATGCCTTA
    GAGGAATTGT CCAAAGCCAC TCAGCGGAAC AGTGCTGAAG AAGGGCTTCC AACAAACAGT
    ACTTTAGACA GCAGACCAAA GAGTAGCAGC CCAATTAGAC TACCTGAGAT CAGTGGAGGA
    CAGACAAACC GTGCGGTAGA AGCAGAACCA CAGCCAACCA AAAAGGCTTC TGGAATGTTG
    TCCTTCTTCC GAGGGACAGC AGGGAAAAGC CCTGACCTCT CTTCCCAGAA GAGAGAGACC
    TTACGTGGAG CAGATAGTGC TTACTACCAA GTGGGGCAGA CGGGCAAGGA GGGGACGGAG
    AATCAAGGCA TTGAGCCTCA AGATGAAGTA GATGGAGGAG ATACACAGAA GAAACAACTT
    ACAAATCCTC ATGTGGAGCT TCAATTTTCG GATGCCAACG CCAAGTTTTA CTGTCGGCTC
    TACTATGCGG GAGAGTTTCA TAAGATGCGA GAAGTGATTC TGGGCAGCAG CGAAGAAGAT
    TTCATTCGTT CCCTTTCTCA CTCTTCTCCG TGGCAGGCCC GAGGAGGCAA ATCAGGAGCT
    GCCTTTTACG CTACTGAAGA TGACAGATTC ATTTTGAAGC AAATGCCTCG TCTGGAAGTC
    CAGTCTTTCC TTGACTTCGC ACCACACTAC TTCAATTACA TTACAAATGC CGTTCAGCAA
    AAGAGGCCTA CTGCATTGGC CAAGATTCTT GGAGTTTACA GAATTGGTTA TAAGAATTCT
    CAGAACAACA CTGAGAAGAA GCTAGATCTC CTTGTCATGG AAAATCTTTT CTACGGGAGA
    AAGATGGCAC AGGTTTTTGA CTTGAAGGGT TCACTTAGGA ATCGAAATGT AAAAACCGAT
    ACCGGGAAAG AGAGCTGTGA TGTTGTTCTT CTGGATGAAA ATCTCTTAAA GATGGTTCGA
    GACAACCCTC TCTATATCCG TTCTCATTCC AAAGCTGTGC TGAGAGCCTC GATCCGGAGC
    GACTCCCACT TCCTTTCTAG CCACCTCATT ATAGACTATT CTTTGCTAGT TGGGCGAGAC
    GATACCAGCA ATGAGCTAGT AGTTGGAATT ATAGATTATA TCCGAACATT TACCTGGGAC
    AAAAAGCTTG AGATGGTTGT GAAATCAACG GGAATTTTAG GAGGACAAGG TAAAATGCCG
    ACTGTGGTCT CTCCAGAGTT GTACAGGACT AGATTCTGTG AAGCCATGGA CAAGTATTTC
    CTGATGGTGC CAGACCACTG GACAGGCTTG GGTCTGAACT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800578.1
    CDS132..6296
    Translation

    Target ORF information:

    RefSeq Version XM_010802276.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802276.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAAGTATT TGATTTCTGA CAATGGAGGA CAACAGCTAT CAATAAGTGA CGCCTTCATC
    AAAGAATCCT TATTTAACCG TCGAGTTGAG GAAAAATCCA AAGAACTGCC TTTCACACCT
    TTGGGCTGGC ATCATAACAA CCTGGAGCTG CTGAGGGAGG AGAATGGAGA GAAACAAGCT
    ATGGAGAGGT TGCTTTCAGC TAATCATAAC CACATGATGG CGCTGCTCCA ACAGTTGCTC
    CATAGTGAGT CATTGTCAAC GTCTTGGAGG GACATCATCG TATCCCTGGT CTGCCAGGTT
    GTTCAGACAG TCCGACCTGA CGTCAAGAAC CGGGATGATG ACATGGATAT CCGCCAGTTT
    GTCCACATCA AAAAAATCCC AGGTGGAAAG AAATTTGATT CTGTGGTTGT CAATGGATTT
    GTTTGCACCA AGAATATTGC ACATAAAAAG ATGAATTCTT CTATTAAAAA CCCCAAAATT
    CTTCTGCTGA AATGTTCCAT TGAATATCTC TACAGAGAAG AAACCAAATT TACTTGCATT
    GATCCTATTG TGCTTCAGGA AAGGGAATTC CTAAAGAATT ATGTTCAGCG AATAGTTGAT
    GTTCGACCCA CACTGGTTCT GGTTGAGAAA ACGGTATCTC GAATTGCACA GGATATGTTG
    CTGGAACACG GCATTACTTT GGTCATTAAT GTAAAATCAC AAGTTTTGGA ACGAGTTAGC
    CGGATGACCC AAGGTGATTT GGTGATGTCA ATGGACCAGC TGCTTACCAA ACCCCACCTG
    GGCACCTGTC ACAAATTTTA CATGCAGATG TTTCAGTTGC CTAATGAACA AACCAAGACA
    CTGATGTTTT TTGAAGGCTG TCCACAGCAC CTGGGCTGTA CAATCAAGCT TAGAGGAGGC
    TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCTTAATAT TTATGATCTG TGTAGCTTAT
    CATTCTCAAC TAGAAATCTC CTTCCTTATG GATGAATTTG CTATGCCTCC TACATTAACG
    CAAAACCCTT CCTTCCATTC TCTGATTGAA GGACAGGAGG ATGAAGGGGC TTCACAGGAG
    CCATTCAGCG GAAGTCCCCT CCCCCGGGAG CCTGACTTCC CCCCAGACTT TCTGCCTTCT
    GATGATGGCA GTTTGCTAGA AACAAGGATT CTGTTTGAGA AGGGTGACCA GGAGAATAAG
    AGTGTGCCAC AGGATGTTGC CTCTTTGAAG CCTCAAGAGC ATGCCCCAGC AGCTTGCCCG
    ATGGGTGCCC CCTGTGCTCT CTTTCCATCA GTACCAGAGT CATTGCTTCC GCTCCATGTG
    GATGACCAAC AGGACGCCAT AGGCAGTGAG CAGCCAGAGG CTTTGCAGCA AACAGAGGAG
    CTTCCGGATC CCACAAGCCA GATGAGAGCC TTTAGAGACC CTCTGCAGGA TGACACTGGC
    CTGTATGTTA CCGAGGAAGT TACCTCTTCT GAAGATAAAC GGAAGACTGA TTCTTTGACC
    TTTAAGCAAG AGTTGAAAGA TGTGATCCTC TGTATCTCGC CAGTCATCAC ATTCCGGGAG
    CCCTTCCTTT TAACTGACAA GGGCATGAGA TGCTCTACCC GAGATTATTT TGCGGAGCAG
    GTTTACTGGT CTCCTCTCCT CAATAAGGAG TTCAAGGAAA TGGAGAGCAG GCGAAAGAAA
    CAGATGCTTA GGGATCTCTC TGGCCTTCAG GGCATGAATG GAAGTGTTCA GGCCAAGTCC
    ATTCAAGTCC TGCCCTCACA TGAGCTCGTG AGCACCAGAA TTGCTGAGCA CCTGGGGGAC
    AGCCAGAGCT TGGGGAGAAT GCTAGCCGAT TATCGGGCCA GAGGAGGAAG AATCCAGCAA
    AAAAACTCAG ACCCTTTTGC TTACTCAAAG GAGGCATCAA GCACCTCAAG TGGAAAATCA
    GGAAGCAGAA CTGAAGGTGA TGAAGAAAAA GGGTTGATTA CCAGTGATGC AGTGTGGTCG
    ACAAAGGTGG ACTGTCTGAA CCCTGTCAAC CACCAGAGGC TGTGTGTGCT CTTCAGCAGC
    TCTTCTGCCC AGTCCAGCAA CGCTCCCAGT GCCTGTGTCA GTCCTTGGAT TGTAACAATG
    GAATTTTATG GAAAAAATGA TCTTACATTA GGAATATTTT TAGAGAGATA CTGTTTCCGG
    CCTTCCTATC AGTGCCCTAG CATGTTCTGT GATACCCCCA TGGTGCACCA CATTCGACGC
    TTTGTCCATG GCCAAGGCTG TGTGCAGATA ATCCTAAAGG AGCTGGATTC TCCAGTACCT
    GGATATCAGC ATACAATTCT CACCTACTCC TGGTGCAGAA TGTGCAAACA GGTAACACCA
    GTTGTTGCTC TTTCCAATGA GTCTTGGTCC ATGTCGTTTG CAAAATACCT GGAACTTAGG
    TTTTATGGGC ACCAGTACAC CCGCAGAGCC AATGCTGAGC CATGTGGTCA CTCCATCCAC
    CACGATTATC ACCAGTATTT CTCCTATAAC CAGATGGTGG CGTCTTTCAG TTATTCTCCC
    ATTCGGCTTC TTGAAGTGTG TGTTCCACTT CCCAAAATAT TCATTAAACG TCAAGTCCCA
    CTAAAGGTGT CCCTTCTTCA GGATCTGAAG GACTTCTTTC AGAAAGTTTC ACAGGTATAT
    CTTGCTGTGG ATGAAAGACT TGCGTCTTTG AAAACTGATA CATTTAGCAA ATCAAGAGAG
    GAAAAAATGG AAGATATCTT TGCACAAAAA GAGATGGAAG AAAGTGAGTT CAAGAGCTGG
    ACTGAGAAGA TGCAGGCGCG GCTCCTGTCT TCCTCCATGG ATACCCCCCA GCAACTGCAG
    TCAGTCTTCG AGTCCCTCAT CGCCAAGAAA CAAAGCCTCT GTGAGGTGCT GCAAGCTTGG
    AATAACAGGC TGCAAGACCT TTTCCAACAA GAAAAGGGTA GGAAGCGGCC TTCAGTTCCT
    CCAAGTCCTG GAAGACTGAG ACAAGGTGAA GAAAGCAAGA TAAGTGCAGT GGATGCATCT
    CCACGGAATG CTTCTCCAGG ACTTCCAAAT GGAGAAAAAG AGGACCGCTT CTTAGCCACC
    CTTTCCAGTC AGAGCTCCAC CAGTTCCCCC CATCTCCAGC TGCCTACTTC TCCTGAAGGC
    GTGCCTGAGC AGGTCATGGG AGGGCCCCCA GATCTCGATA CTGCCAGCAG TTCTGAAGAT
    GTATTTGATG GACATTTGCT GGGATCTACA GACAGCCAGG TGAAGGAAAA ATCAACCATG
    AAAGCCATCT TTGCAAATTT GCTTCCAGGA AATAGCTATA ACCCTATTCC ATTTCCTTTC
    GATCCAGATA AACACTACTT AATGTATGAA CATGAACGAG TGCCCATTGC AGTCTGTGAG
    AAGGAGCCCA GCTCCATCAT TGCTTTTGCT CTCAGTTGTA AAGAATACCG GAATGCCTTA
    GAGGAATTGT CCAAAGCCAC TCAGCGGAAC AGTGCTGAAG AAGGGCTTCC AACAAACAGT
    ACTTTAGACA GCAGACCAAA GAGTAGCAGC CCAATTAGAC TACCTGAGAT CAGTGGAGGA
    CAGACAAACC GTGCGGTAGA AGCAGAACCA CAGCCAACCA AAAAGGCTTC TGGAATGTTG
    TCCTTCTTCC GAGGGACAGC AGGGAAAAGC CCTGACCTCT CTTCCCAGAA GAGAGAGACC
    TTACGTGGAG CAGATAGTGC TTACTACCAA GTGGGGCAGA CGGGCAAGGA GGGGACGGAG
    AATCAAGGCA TTGAGCCTCA AGATGAAGTA GATGGAGGAG ATACACAGAA GAAACAACTT
    ACAAATCCTC ATGTGGAGCT TCAATTTTCG GATGCCAACG CCAAGTTTTA CTGTCGGCTC
    TACTATGCGG GAGAGTTTCA TAAGATGCGA GAAGTGATTC TGGGCAGCAG CGAAGAAGAT
    TTCATTCGTT CCCTTTCTCA CTCTTCTCCG TGGCAGGCCC GAGGAGGCAA ATCAGGAGCT
    GCCTTTTACG CTACTGAAGA TGACAGATTC ATTTTGAAGC AAATGCCTCG TCTGGAAGTC
    CAGTCTTTCC TTGACTTCGC ACCACACTAC TTCAATTACA TTACAAATGC CGTTCAGCAA
    AAGAGGCCTA CTGCATTGGC CAAGATTCTT GGAGTTTACA GAATTGGTTA TAAGAATTCT
    CAGAACAACA CTGAGAAGAA GCTAGATCTC CTTGTCATGG AAAATCTTTT CTACGGGAGA
    AAGATGGCAC AGGTTTTTGA CTTGAAGGGT TCACTTAGGA ATCGAAATGT AAAAACCGAT
    ACCGGGAAAG AGAGCTGTGA TGTTGTTCTT CTGGATGAAA ATCTCTTAAA GATGGTTCGA
    GACAACCCTC TCTATATCCG TTCTCATTCC AAAGCTGTGC TGAGAGCCTC GATCCGGAGC
    GACTCCCACT TCCTTTCTAG CCACCTCATT ATAGACTATT CTTTGCTAGT TGGGCGAGAC
    GATACCAGCA ATGAGCTAGT AGTTGGAATT ATAGATTATA TCCGAACATT TACCTGGGAC
    AAAAAGCTTG AGATGGTTGT GAAATCAACG GGAATTTTAG GAGGACAAGG TAAAATGCCG
    ACTGTGGTCT CTCCAGAGTT GTACAGGACT AGATTCTGTG AAGCCATGGA CAAGTATTTC
    CTGATGGTGC CAGACCACTG GACAGGCTTG GGTCTGAACT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58665
    Clone ID Related Accession (Same CDS sequence) XM_010802276.2 , XM_024978647.1
    Accession Version XM_024978647.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAAGTATT TGATTTCTGA CAATGGAGGA CAACAGCTAT CAATAAGTGA CGCCTTCATC
    AAAGAATCCT TATTTAACCG TCGAGTTGAG GAAAAATCCA AAGAACTGCC TTTCACACCT
    TTGGGCTGGC ATCATAACAA CCTGGAGCTG CTGAGGGAGG AGAATGGAGA GAAACAAGCT
    ATGGAGAGGT TGCTTTCAGC TAATCATAAC CACATGATGG CGCTGCTCCA ACAGTTGCTC
    CATAGTGAGT CATTGTCAAC GTCTTGGAGG GACATCATCG TATCCCTGGT CTGCCAGGTT
    GTTCAGACAG TCCGACCTGA CGTCAAGAAC CGGGATGATG ACATGGATAT CCGCCAGTTT
    GTCCACATCA AAAAAATCCC AGGTGGAAAG AAATTTGATT CTGTGGTTGT CAATGGATTT
    GTTTGCACCA AGAATATTGC ACATAAAAAG ATGAATTCTT CTATTAAAAA CCCCAAAATT
    CTTCTGCTGA AATGTTCCAT TGAATATCTC TACAGAGAAG AAACCAAATT TACTTGCATT
    GATCCTATTG TGCTTCAGGA AAGGGAATTC CTAAAGAATT ATGTTCAGCG AATAGTTGAT
    GTTCGACCCA CACTGGTTCT GGTTGAGAAA ACGGTATCTC GAATTGCACA GGATATGTTG
    CTGGAACACG GCATTACTTT GGTCATTAAT GTAAAATCAC AAGTTTTGGA ACGAGTTAGC
    CGGATGACCC AAGGTGATTT GGTGATGTCA ATGGACCAGC TGCTTACCAA ACCCCACCTG
    GGCACCTGTC ACAAATTTTA CATGCAGATG TTTCAGTTGC CTAATGAACA AACCAAGACA
    CTGATGTTTT TTGAAGGCTG TCCACAGCAC CTGGGCTGTA CAATCAAGCT TAGAGGAGGC
    TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCTTAATAT TTATGATCTG TGTAGCTTAT
    CATTCTCAAC TAGAAATCTC CTTCCTTATG GATGAATTTG CTATGCCTCC TACATTAACG
    CAAAACCCTT CCTTCCATTC TCTGATTGAA GGACAGGAGG ATGAAGGGGC TTCACAGGAG
    CCATTCAGCG GAAGTCCCCT CCCCCGGGAG CCTGACTTCC CCCCAGACTT TCTGCCTTCT
    GATGATGGCA GTTTGCTAGA AACAAGGATT CTGTTTGAGA AGGGTGACCA GGAGAATAAG
    AGTGTGCCAC AGGATGTTGC CTCTTTGAAG CCTCAAGAGC ATGCCCCAGC AGCTTGCCCG
    ATGGGTGCCC CCTGTGCTCT CTTTCCATCA GTACCAGAGT CATTGCTTCC GCTCCATGTG
    GATGACCAAC AGGACGCCAT AGGCAGTGAG CAGCCAGAGG CTTTGCAGCA AACAGAGGAG
    CTTCCGGATC CCACAAGCCA GATGAGAGCC TTTAGAGACC CTCTGCAGGA TGACACTGGC
    CTGTATGTTA CCGAGGAAGT TACCTCTTCT GAAGATAAAC GGAAGACTGA TTCTTTGACC
    TTTAAGCAAG AGTTGAAAGA TGTGATCCTC TGTATCTCGC CAGTCATCAC ATTCCGGGAG
    CCCTTCCTTT TAACTGACAA GGGCATGAGA TGCTCTACCC GAGATTATTT TGCGGAGCAG
    GTTTACTGGT CTCCTCTCCT CAATAAGGAG TTCAAGGAAA TGGAGAGCAG GCGAAAGAAA
    CAGATGCTTA GGGATCTCTC TGGCCTTCAG GGCATGAATG GAAGTGTTCA GGCCAAGTCC
    ATTCAAGTCC TGCCCTCACA TGAGCTCGTG AGCACCAGAA TTGCTGAGCA CCTGGGGGAC
    AGCCAGAGCT TGGGGAGAAT GCTAGCCGAT TATCGGGCCA GAGGAGGAAG AATCCAGCAA
    AAAAACTCAG ACCCTTTTGC TTACTCAAAG GAGGCATCAA GCACCTCAAG TGGAAAATCA
    GGAAGCAGAA CTGAAGGTGA TGAAGAAAAA GGGTTGATTA CCAGTGATGC AGTGTGGTCG
    ACAAAGGTGG ACTGTCTGAA CCCTGTCAAC CACCAGAGGC TGTGTGTGCT CTTCAGCAGC
    TCTTCTGCCC AGTCCAGCAA CGCTCCCAGT GCCTGTGTCA GTCCTTGGAT TGTAACAATG
    GAATTTTATG GAAAAAATGA TCTTACATTA GGAATATTTT TAGAGAGATA CTGTTTCCGG
    CCTTCCTATC AGTGCCCTAG CATGTTCTGT GATACCCCCA TGGTGCACCA CATTCGACGC
    TTTGTCCATG GCCAAGGCTG TGTGCAGATA ATCCTAAAGG AGCTGGATTC TCCAGTACCT
    GGATATCAGC ATACAATTCT CACCTACTCC TGGTGCAGAA TGTGCAAACA GGTAACACCA
    GTTGTTGCTC TTTCCAATGA GTCTTGGTCC ATGTCGTTTG CAAAATACCT GGAACTTAGG
    TTTTATGGGC ACCAGTACAC CCGCAGAGCC AATGCTGAGC CATGTGGTCA CTCCATCCAC
    CACGATTATC ACCAGTATTT CTCCTATAAC CAGATGGTGG CGTCTTTCAG TTATTCTCCC
    ATTCGGCTTC TTGAAGTGTG TGTTCCACTT CCCAAAATAT TCATTAAACG TCAAGTCCCA
    CTAAAGGTGT CCCTTCTTCA GGATCTGAAG GACTTCTTTC AGAAAGTTTC ACAGGTATAT
    CTTGCTGTGG ATGAAAGACT TGCGTCTTTG AAAACTGATA CATTTAGCAA ATCAAGAGAG
    GAAAAAATGG AAGATATCTT TGCACAAAAA GAGATGGAAG AAAGTGAGTT CAAGAGCTGG
    ACTGAGAAGA TGCAGGCGCG GCTCCTGTCT TCCTCCATGG ATACCCCCCA GCAACTGCAG
    TCAGTCTTCG AGTCCCTCAT CGCCAAGAAA CAAAGCCTCT GTGAGGTGCT GCAAGCTTGG
    AATAACAGGC TGCAAGACCT TTTCCAACAA GAAAAGGGTA GGAAGCGGCC TTCAGTTCCT
    CCAAGTCCTG GAAGACTGAG ACAAGGTGAA GAAAGCAAGA TAAGTGCAGT GGATGCATCT
    CCACGGAATG CTTCTCCAGG ACTTCCAAAT GGAGAAAAAG AGGACCGCTT CTTAGCCACC
    CTTTCCAGTC AGAGCTCCAC CAGTTCCCCC CATCTCCAGC TGCCTACTTC TCCTGAAGGC
    GTGCCTGAGC AGGTCATGGG AGGGCCCCCA GATCTCGATA CTGCCAGCAG TTCTGAAGAT
    GTATTTGATG GACATTTGCT GGGATCTACA GACAGCCAGG TGAAGGAAAA ATCAACCATG
    AAAGCCATCT TTGCAAATTT GCTTCCAGGA AATAGCTATA ACCCTATTCC ATTTCCTTTC
    GATCCAGATA AACACTACTT AATGTATGAA CATGAACGAG TGCCCATTGC AGTCTGTGAG
    AAGGAGCCCA GCTCCATCAT TGCTTTTGCT CTCAGTTGTA AAGAATACCG GAATGCCTTA
    GAGGAATTGT CCAAAGCCAC TCAGCGGAAC AGTGCTGAAG AAGGGCTTCC AACAAACAGT
    ACTTTAGACA GCAGACCAAA GAGTAGCAGC CCAATTAGAC TACCTGAGAT CAGTGGAGGA
    CAGACAAACC GTGCGGTAGA AGCAGAACCA CAGCCAACCA AAAAGGCTTC TGGAATGTTG
    TCCTTCTTCC GAGGGACAGC AGGGAAAAGC CCTGACCTCT CTTCCCAGAA GAGAGAGACC
    TTACGTGGAG CAGATAGTGC TTACTACCAA GTGGGGCAGA CGGGCAAGGA GGGGACGGAG
    AATCAAGGCA TTGAGCCTCA AGATGAAGTA GATGGAGGAG ATACACAGAA GAAACAACTT
    ACAAATCCTC ATGTGGAGCT TCAATTTTCG GATGCCAACG CCAAGTTTTA CTGTCGGCTC
    TACTATGCGG GAGAGTTTCA TAAGATGCGA GAAGTGATTC TGGGCAGCAG CGAAGAAGAT
    TTCATTCGTT CCCTTTCTCA CTCTTCTCCG TGGCAGGCCC GAGGAGGCAA ATCAGGAGCT
    GCCTTTTACG CTACTGAAGA TGACAGATTC ATTTTGAAGC AAATGCCTCG TCTGGAAGTC
    CAGTCTTTCC TTGACTTCGC ACCACACTAC TTCAATTACA TTACAAATGC CGTTCAGCAA
    AAGAGGCCTA CTGCATTGGC CAAGATTCTT GGAGTTTACA GAATTGGTTA TAAGAATTCT
    CAGAACAACA CTGAGAAGAA GCTAGATCTC CTTGTCATGG AAAATCTTTT CTACGGGAGA
    AAGATGGCAC AGGTTTTTGA CTTGAAGGGT TCACTTAGGA ATCGAAATGT AAAAACCGAT
    ACCGGGAAAG AGAGCTGTGA TGTTGTTCTT CTGGATGAAA ATCTCTTAAA GATGGTTCGA
    GACAACCCTC TCTATATCCG TTCTCATTCC AAAGCTGTGC TGAGAGCCTC GATCCGGAGC
    GACTCCCACT TCCTTTCTAG CCACCTCATT ATAGACTATT CTTTGCTAGT TGGGCGAGAC
    GATACCAGCA ATGAGCTAGT AGTTGGAATT ATAGATTATA TCCGAACATT TACCTGGGAC
    AAAAAGCTTG AGATGGTTGT GAAATCAACG GGAATTTTAG GAGGACAAGG TAAAATGCCG
    ACTGTGGTCT CTCCAGAGTT GTACAGGACT AGATTCTGTG AAGCCATGGA CAAGTATTTC
    CTGATGGTGC CAGACCACTG GACAGGCTTG GGTCTGAACT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834415.1
    CDS155..6319
    Translation

    Target ORF information:

    RefSeq Version XM_024978647.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978647.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGCAGAGGAC AGTTTGCAAC ACCCCCAGGA GAACTCAGAC
    ACCAGAAGGA AGGCAGAACC TACTTTTGGA GGTCATGACC CTCGCACAGC TGTTCAACTC
    CGAAGCCTCA GCACAGTATT AAAACGCCTC AAGGAAATCA TGGAGGGGAA GAGCCAGGAT
    AGTGACCTGA AGCAATACTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGTAAT
    GAGAAATTTA CCACTTTTAG GCGCAGACAC CACTGCCGAC TCTGTGGGCA GATTTTCTGC
    AGTCGTTGCT GCAATCAAGA AATCCCTGGA AAATTTATGG GTTATACAGG GGACCTCCGA
    GCCTGTACAT ACTGTAGAAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC
    ATTGGTGAAG ACTTGAATGC TCTTTCAGAC TCTGCTTCCT CTGTGTCTGT ACTTGATCCA
    AATGAACCCC GTACACCTGT GGGGAGTAGA AAAGCCAGCC GCAACATATT TTTAGAGGAT
    GATTTTGCCT GGCAAAGTTT AATTCATCCA GACTCCTCAA ACACTGCTCT TTCAACAAGA
    CTTGTATCTG TTCAAGAGGA TGCTGGGAAA TCTCCTGCTC GAAATAGATC AGCCAGCATT
    ACTAATCTGT CACTGGATCG ATCTGCGTCT CCAATGGTAC CATCATACGA GACGTCTGTC
    AGTCCCCAGG CTAACCGAAC GTATGTTCGG ACAGAGACCA CTGAGGATGA ACGCAAAATT
    CTTCTGGACA GTGTTCAGTT AAAAGACCTA TGGAAGAAAA TCTGCCATCA CAGCAGTGGA
    ATGGAGTTTC AGGATCACCG CTACTGGCTG AGAACACACC CCAACTGCAT TGTAGGAAAG
    GAGTTAGTCA ACTGGTTAAT CCGAAATGGA CACATTGCTA CAAGGGCACA GGCTATAGCA
    ATTGGACAGG CAATGGTGGA TGGACGTTGG CTGGATTGTG TCAGTCACCA CGACCAGCTC
    TTCAGGGATG AGTATGCGCT GTACAGGCCG CTGCAGAGTA CAGAATTTTC CGAGACCCCT
    TCTCCGGACA GTGACTCGGT GAACTCTGTG GAAGGACACT CTGAGCCATC CTGGTTTAAA
    GACATCAAGT TTGATGACAG TGACACTGAG CAGATAGCTG AAGAAGGTGA CGATAATTTG
    ACTAAGTATT TGATTTCTGA CAATGGAGGA CAACAGCTAT CAATAAGTGA CGCCTTCATC
    AAAGAATCCT TATTTAACCG TCGAGTTGAG GAAAAATCCA AAGAACTGCC TTTCACACCT
    TTGGGCTGGC ATCATAACAA CCTGGAGCTG CTGAGGGAGG AGAATGGAGA GAAACAAGCT
    ATGGAGAGGT TGCTTTCAGC TAATCATAAC CACATGATGG CGCTGCTCCA ACAGTTGCTC
    CATAGTGAGT CATTGTCAAC GTCTTGGAGG GACATCATCG TATCCCTGGT CTGCCAGGTT
    GTTCAGACAG TCCGACCTGA CGTCAAGAAC CGGGATGATG ACATGGATAT CCGCCAGTTT
    GTCCACATCA AAAAAATCCC AGGTGGAAAG AAATTTGATT CTGTGGTTGT CAATGGATTT
    GTTTGCACCA AGAATATTGC ACATAAAAAG ATGAATTCTT CTATTAAAAA CCCCAAAATT
    CTTCTGCTGA AATGTTCCAT TGAATATCTC TACAGAGAAG AAACCAAATT TACTTGCATT
    GATCCTATTG TGCTTCAGGA AAGGGAATTC CTAAAGAATT ATGTTCAGCG AATAGTTGAT
    GTTCGACCCA CACTGGTTCT GGTTGAGAAA ACGGTATCTC GAATTGCACA GGATATGTTG
    CTGGAACACG GCATTACTTT GGTCATTAAT GTAAAATCAC AAGTTTTGGA ACGAGTTAGC
    CGGATGACCC AAGGTGATTT GGTGATGTCA ATGGACCAGC TGCTTACCAA ACCCCACCTG
    GGCACCTGTC ACAAATTTTA CATGCAGATG TTTCAGTTGC CTAATGAACA AACCAAGACA
    CTGATGTTTT TTGAAGGCTG TCCACAGCAC CTGGGCTGTA CAATCAAGCT TAGAGGAGGC
    TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCTTAATAT TTATGATCTG TGTAGCTTAT
    CATTCTCAAC TAGAAATCTC CTTCCTTATG GATGAATTTG CTATGCCTCC TACATTAACG
    CAAAACCCTT CCTTCCATTC TCTGATTGAA GGACAGGAGG ATGAAGGGGC TTCACAGGAG
    CCATTCAGCG GAAGTCCCCT CCCCCGGGAG CCTGACTTCC CCCCAGACTT TCTGCCTTCT
    GATGATGGCA GTTTGCTAGA AACAAGGATT CTGTTTGAGA AGGGTGACCA GGAGAATAAG
    AGTGTGCCAC AGGATGTTGC CTCTTTGAAG CCTCAAGAGC ATGCCCCAGC AGCTTGCCCG
    ATGGGTGCCC CCTGTGCTCT CTTTCCATCA GTACCAGAGT CATTGCTTCC GCTCCATGTG
    GATGACCAAC AGGACGCCAT AGGCAGTGAG CAGCCAGAGG CTTTGCAGCA AACAGAGGAG
    CTTCCGGATC CCACAAGCCA GATGAGAGCC TTTAGAGACC CTCTGCAGGA TGACACTGGC
    CTGTATGTTA CCGAGGAAGT TACCTCTTCT GAAGATAAAC GGAAGACTGA TTCTTTGACC
    TTTAAGCAAG AGTTGAAAGA TGTGATCCTC TGTATCTCGC CAGTCATCAC ATTCCGGGAG
    CCCTTCCTTT TAACTGACAA GGGCATGAGA TGCTCTACCC GAGATTATTT TGCGGAGCAG
    GTTTACTGGT CTCCTCTCCT CAATAAGGAG TTCAAGGAAA TGGAGAGCAG GCGAAAGAAA
    CAGATGCTTA GGGATCTCTC TGGCCTTCAG GGCATGAATG GAAGTGTTCA GGCCAAGTCC
    ATTCAAGTCC TGCCCTCACA TGAGCTCGTG AGCACCAGAA TTGCTGAGCA CCTGGGGGAC
    AGCCAGAGCT TGGGGAGAAT GCTAGCCGAT TATCGGGCCA GAGGAGGAAG AATCCAGCAA
    AAAAACTCAG ACCCTTTTGC TTACTCAAAG GAGGCATCAA GCACCTCAAG TGGAAAATCA
    GGAAGCAGAA CTGAAGGTGA TGAAGAAAAA GGGTTGATTA CCAGTGATGC AGTGTGGTCG
    ACAAAGGTGG ACTGTCTGAA CCCTGTCAAC CACCAGAGGC TGTGTGTGCT CTTCAGCAGC
    TCTTCTGCCC AGTCCAGCAA CGCTCCCAGT GCCTGTGTCA GTCCTTGGAT TGTAACAATG
    GAATTTTATG GAAAAAATGA TCTTACATTA GGAATATTTT TAGAGAGATA CTGTTTCCGG
    CCTTCCTATC AGTGCCCTAG CATGTTCTGT GATACCCCCA TGGTGCACCA CATTCGACGC
    TTTGTCCATG GCCAAGGCTG TGTGCAGATA ATCCTAAAGG AGCTGGATTC TCCAGTACCT
    GGATATCAGC ATACAATTCT CACCTACTCC TGGTGCAGAA TGTGCAAACA GGTAACACCA
    GTTGTTGCTC TTTCCAATGA GTCTTGGTCC ATGTCGTTTG CAAAATACCT GGAACTTAGG
    TTTTATGGGC ACCAGTACAC CCGCAGAGCC AATGCTGAGC CATGTGGTCA CTCCATCCAC
    CACGATTATC ACCAGTATTT CTCCTATAAC CAGATGGTGG CGTCTTTCAG TTATTCTCCC
    ATTCGGCTTC TTGAAGTGTG TGTTCCACTT CCCAAAATAT TCATTAAACG TCAAGTCCCA
    CTAAAGGTGT CCCTTCTTCA GGATCTGAAG GACTTCTTTC AGAAAGTTTC ACAGGTATAT
    CTTGCTGTGG ATGAAAGACT TGCGTCTTTG AAAACTGATA CATTTAGCAA ATCAAGAGAG
    GAAAAAATGG AAGATATCTT TGCACAAAAA GAGATGGAAG AAAGTGAGTT CAAGAGCTGG
    ACTGAGAAGA TGCAGGCGCG GCTCCTGTCT TCCTCCATGG ATACCCCCCA GCAACTGCAG
    TCAGTCTTCG AGTCCCTCAT CGCCAAGAAA CAAAGCCTCT GTGAGGTGCT GCAAGCTTGG
    AATAACAGGC TGCAAGACCT TTTCCAACAA GAAAAGGGTA GGAAGCGGCC TTCAGTTCCT
    CCAAGTCCTG GAAGACTGAG ACAAGGTGAA GAAAGCAAGA TAAGTGCAGT GGATGCATCT
    CCACGGAATG CTTCTCCAGG ACTTCCAAAT GGAGAAAAAG AGGACCGCTT CTTAGCCACC
    CTTTCCAGTC AGAGCTCCAC CAGTTCCCCC CATCTCCAGC TGCCTACTTC TCCTGAAGGC
    GTGCCTGAGC AGGTCATGGG AGGGCCCCCA GATCTCGATA CTGCCAGCAG TTCTGAAGAT
    GTATTTGATG GACATTTGCT GGGATCTACA GACAGCCAGG TGAAGGAAAA ATCAACCATG
    AAAGCCATCT TTGCAAATTT GCTTCCAGGA AATAGCTATA ACCCTATTCC ATTTCCTTTC
    GATCCAGATA AACACTACTT AATGTATGAA CATGAACGAG TGCCCATTGC AGTCTGTGAG
    AAGGAGCCCA GCTCCATCAT TGCTTTTGCT CTCAGTTGTA AAGAATACCG GAATGCCTTA
    GAGGAATTGT CCAAAGCCAC TCAGCGGAAC AGTGCTGAAG AAGGGCTTCC AACAAACAGT
    ACTTTAGACA GCAGACCAAA GAGTAGCAGC CCAATTAGAC TACCTGAGAT CAGTGGAGGA
    CAGACAAACC GTGCGGTAGA AGCAGAACCA CAGCCAACCA AAAAGGCTTC TGGAATGTTG
    TCCTTCTTCC GAGGGACAGC AGGGAAAAGC CCTGACCTCT CTTCCCAGAA GAGAGAGACC
    TTACGTGGAG CAGATAGTGC TTACTACCAA GTGGGGCAGA CGGGCAAGGA GGGGACGGAG
    AATCAAGGCA TTGAGCCTCA AGATGAAGTA GATGGAGGAG ATACACAGAA GAAACAACTT
    ACAAATCCTC ATGTGGAGCT TCAATTTTCG GATGCCAACG CCAAGTTTTA CTGTCGGCTC
    TACTATGCGG GAGAGTTTCA TAAGATGCGA GAAGTGATTC TGGGCAGCAG CGAAGAAGAT
    TTCATTCGTT CCCTTTCTCA CTCTTCTCCG TGGCAGGCCC GAGGAGGCAA ATCAGGAGCT
    GCCTTTTACG CTACTGAAGA TGACAGATTC ATTTTGAAGC AAATGCCTCG TCTGGAAGTC
    CAGTCTTTCC TTGACTTCGC ACCACACTAC TTCAATTACA TTACAAATGC CGTTCAGCAA
    AAGAGGCCTA CTGCATTGGC CAAGATTCTT GGAGTTTACA GAATTGGTTA TAAGAATTCT
    CAGAACAACA CTGAGAAGAA GCTAGATCTC CTTGTCATGG AAAATCTTTT CTACGGGAGA
    AAGATGGCAC AGGTTTTTGA CTTGAAGGGT TCACTTAGGA ATCGAAATGT AAAAACCGAT
    ACCGGGAAAG AGAGCTGTGA TGTTGTTCTT CTGGATGAAA ATCTCTTAAA GATGGTTCGA
    GACAACCCTC TCTATATCCG TTCTCATTCC AAAGCTGTGC TGAGAGCCTC GATCCGGAGC
    GACTCCCACT TCCTTTCTAG CCACCTCATT ATAGACTATT CTTTGCTAGT TGGGCGAGAC
    GATACCAGCA ATGAGCTAGT AGTTGGAATT ATAGATTATA TCCGAACATT TACCTGGGAC
    AAAAAGCTTG AGATGGTTGT GAAATCAACG GGAATTTTAG GAGGACAAGG TAAAATGCCG
    ACTGTGGTCT CTCCAGAGTT GTACAGGACT AGATTCTGTG AAGCCATGGA CAAGTATTTC
    CTGATGGTGC CAGACCACTG GACAGGCTTG GGTCTGAACT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58666
    Clone ID Related Accession (Same CDS sequence) XM_024978654.1 , XM_010802277.2
    Accession Version XM_024978654.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6162bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGAGGACAGT TTGCAACACC CCCAGGAGAA CTCAGACACC
    AGAAGGAAGG CAGAACCTAC TTTTGGAGGT CATGACCCTC GCACAGCTGT TCAACTCCGA
    AGCCTCAGCA CAGTATTAAA ACGCCTCAAG GAAATCATGG AGGGGAAGAG CCAGGATAGT
    GACCTGAAGC AATACTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGTAATGAG
    AAATTTACCA CTTTTAGGCG CAGACACCAC TGCCGACTCT GTGGGCAGAT TTTCTGCAGT
    CGTTGCTGCA ATCAAGAAAT CCCTGGAAAA TTTATGGGTT ATACAGGGGA CCTCCGAGCC
    TGTACATACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCCATT
    GGTGAAGACT TGAATGCTCT TTCAGACTCT GCTTCCTCTG TGTCTGTACT TGATCCAAAT
    GAACCCCGTA CACCTGTGGG GAGTAGAAAA GCCAGCCGCA ACATATTTTT AGAGGATGAT
    TTTGCCTGGC AAAGTTTAAT TCATCCAGAC TCCTCAAACA CTGCTCTTTC AACAAGACTT
    GTATCTGTTC AAGAGGATGC TGGGAAATCT CCTGCTCGAA ATAGATCAGC CAGCATTACT
    AATCTGTCAC TGGATCGATC TGCGTCTCCA ATGGTACCAT CATACGAGAC GTCTGTCAGT
    CCCCAGGCTA ACCGAACGTA TGTTCGGACA GAGACCACTG AGGATGAACG CAAAATTCTT
    CTGGACAGTG TTCAGTTAAA AGACCTATGG AAGAAAATCT GCCATCACAG CAGTGGAATG
    GAGTTTCAGG ATCACCGCTA CTGGCTGAGA ACACACCCCA ACTGCATTGT AGGAAAGGAG
    TTAGTCAACT GGTTAATCCG AAATGGACAC ATTGCTACAA GGGCACAGGC TATAGCAATT
    GGACAGGCAA TGGTGGATGG ACGTTGGCTG GATTGTGTCA GTCACCACGA CCAGCTCTTC
    AGGGATGAGT ATGCGCTGTA CAGGCCGCTG CAGAGTACAG AATTTTCCGA GACCCCTTCT
    CCGGACAGTG ACTCGGTGAA CTCTGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC
    ATCAAGTTTG ATGACAGTGA CACTGAGCAG ATAGCTGAAG AAGGTGACGA TAATTTGACT
    AAGTATTTGA TTTCTGACAA TGGAGGACAA CAGCTATCAA TAAGTGACGC CTTCATCAAA
    GAATCCTTAT TTAACCGTCG AGTTGAGGAA AAATCCAAAG AACTGCCTTT CACACCTTTG
    GGCTGGCATC ATAACAACCT GGAGCTGCTG AGGGAGGAGA ATGGAGAGAA ACAAGCTATG
    GAGAGGTTGC TTTCAGCTAA TCATAACCAC ATGATGGCGC TGCTCCAACA GTTGCTCCAT
    AGTGAGTCAT TGTCAACGTC TTGGAGGGAC ATCATCGTAT CCCTGGTCTG CCAGGTTGTT
    CAGACAGTCC GACCTGACGT CAAGAACCGG GATGATGACA TGGATATCCG CCAGTTTGTC
    CACATCAAAA AAATCCCAGG TGGAAAGAAA TTTGATTCTG TGGTTGTCAA TGGATTTGTT
    TGCACCAAGA ATATTGCACA TAAAAAGATG AATTCTTCTA TTAAAAACCC CAAAATTCTT
    CTGCTGAAAT GTTCCATTGA ATATCTCTAC AGAGAAGAAA CCAAATTTAC TTGCATTGAT
    CCTATTGTGC TTCAGGAAAG GGAATTCCTA AAGAATTATG TTCAGCGAAT AGTTGATGTT
    CGACCCACAC TGGTTCTGGT TGAGAAAACG GTATCTCGAA TTGCACAGGA TATGTTGCTG
    GAACACGGCA TTACTTTGGT CATTAATGTA AAATCACAAG TTTTGGAACG AGTTAGCCGG
    ATGACCCAAG GTGATTTGGT GATGTCAATG GACCAGCTGC TTACCAAACC CCACCTGGGC
    ACCTGTCACA AATTTTACAT GCAGATGTTT CAGTTGCCTA ATGAACAAAC CAAGACACTG
    ATGTTTTTTG AAGGCTGTCC ACAGCACCTG GGCTGTACAA TCAAGCTTAG AGGAGGCTCT
    GATTATGAGC TGGCTCGAGT TAAGGAGATC TTAATATTTA TGATCTGTGT AGCTTATCAT
    TCTCAACTAG AAATCTCCTT CCTTATGGAT GAATTTGCTA TGCCTCCTAC ATTAACGCAA
    AACCCTTCCT TCCATTCTCT GATTGAAGGA CAGGAGGATG AAGGGGCTTC ACAGGAGCCA
    TTCAGCGGAA GTCCCCTCCC CCGGGAGCCT GACTTCCCCC CAGACTTTCT GCCTTCTGAT
    GATGGCAGTT TGCTAGAAAC AAGGATTCTG TTTGAGAAGG GTGACCAGGA GAATAAGAGT
    GTGCCACAGG ATGTTGCCTC TTTGAAGCCT CAAGAGCATG CCCCAGCAGC TTGCCCGATG
    GGTGCCCCCT GTGCTCTCTT TCCATCAGTA CCAGAGTCAT TGCTTCCGCT CCATGTGGAT
    GACCAACAGG ACGCCATAGG CAGTGAGCAG CCAGAGGCTT TGCAGCAAAC AGAGGAGCTT
    CCGGATCCCA CAAGCCAGAT GAGAGCCTTT AGAGACCCTC TGCAGGATGA CACTGGCCTG
    TATGTTACCG AGGAAGTTAC CTCTTCTGAA GATAAACGGA AGACTGATTC TTTGACCTTT
    AAGCAAGAGT TGAAAGATGT GATCCTCTGT ATCTCGCCAG TCATCACATT CCGGGAGCCC
    TTCCTTTTAA CTGACAAGGG CATGAGATGC TCTACCCGAG ATTATTTTGC GGAGCAGGTT
    TACTGGTCTC CTCTCCTCAA TAAGGAGTTC AAGGAAATGG AGAGCAGGCG AAAGAAACAG
    ATGCTTAGGG ATCTCTCTGG CCTTCAGGGC ATGAATGGAA GTGTTCAGGC CAAGTCCATT
    CAAGTCCTGC CCTCACATGA GCTCGTGAGC ACCAGAATTG CTGAGCACCT GGGGGACAGC
    CAGAGCTTGG GGAGAATGCT AGCCGATTAT CGGGCCAGAG GAGGAAGAAT CCAGCAAAAA
    AACTCAGACC CTTTTGCTTA CTCAAAGGAG GCATCAAGCA CCTCAAGTGG AAAATCAGGA
    AGCAGAACTG AAGGTGATGA AGAAAAAGGG TTGATTACCA GTGATGCAGT GTGGTCGACA
    AAGGTGGACT GTCTGAACCC TGTCAACCAC CAGAGGCTGT GTGTGCTCTT CAGCAGCTCT
    TCTGCCCAGT CCAGCAACGC TCCCAGTGCC TGTGTCAGTC CTTGGATTGT AACAATGGAA
    TTTTATGGAA AAAATGATCT TACATTAGGA ATATTTTTAG AGAGATACTG TTTCCGGCCT
    TCCTATCAGT GCCCTAGCAT GTTCTGTGAT ACCCCCATGG TGCACCACAT TCGACGCTTT
    GTCCATGGCC AAGGCTGTGT GCAGATAATC CTAAAGGAGC TGGATTCTCC AGTACCTGGA
    TATCAGCATA CAATTCTCAC CTACTCCTGG TGCAGAATGT GCAAACAGGT AACACCAGTT
    GTTGCTCTTT CCAATGAGTC TTGGTCCATG TCGTTTGCAA AATACCTGGA ACTTAGGTTT
    TATGGGCACC AGTACACCCG CAGAGCCAAT GCTGAGCCAT GTGGTCACTC CATCCACCAC
    GATTATCACC AGTATTTCTC CTATAACCAG ATGGTGGCGT CTTTCAGTTA TTCTCCCATT
    CGGCTTCTTG AAGTGTGTGT TCCACTTCCC AAAATATTCA TTAAACGTCA AGTCCCACTA
    AAGGTGTCCC TTCTTCAGGA TCTGAAGGAC TTCTTTCAGA AAGTTTCACA GGTATATCTT
    GCTGTGGATG AAAGACTTGC GTCTTTGAAA ACTGATACAT TTAGCAAATC AAGAGAGGAA
    AAAATGGAAG ATATCTTTGC ACAAAAAGAG ATGGAAGAAA GTGAGTTCAA GAGCTGGACT
    GAGAAGATGC AGGCGCGGCT CCTGTCTTCC TCCATGGATA CCCCCCAGCA ACTGCAGTCA
    GTCTTCGAGT CCCTCATCGC CAAGAAACAA AGCCTCTGTG AGGTGCTGCA AGCTTGGAAT
    AACAGGCTGC AAGACCTTTT CCAACAAGAA AAGGGTAGGA AGCGGCCTTC AGTTCCTCCA
    AGTCCTGGAA GACTGAGACA AGGTGAAGAA AGCAAGATAA GTGCAGTGGA TGCATCTCCA
    CGGAATGCTT CTCCAGGACT TCCAAATGGA GAAAAAGAGG ACCGCTTCTT AGCCACCCTT
    TCCAGTCAGA GCTCCACCAG TTCCCCCCAT CTCCAGCTGC CTACTTCTCC TGAAGGCGTG
    CCTGAGCAGG TCATGGGAGG GCCCCCAGAT CTCGATACTG CCAGCAGTTC TGAAGATGTA
    TTTGATGGAC ATTTGCTGGG ATCTACAGAC AGCCAGGTGA AGGAAAAATC AACCATGAAA
    GCCATCTTTG CAAATTTGCT TCCAGGAAAT AGCTATAACC CTATTCCATT TCCTTTCGAT
    CCAGATAAAC ACTACTTAAT GTATGAACAT GAACGAGTGC CCATTGCAGT CTGTGAGAAG
    GAGCCCAGCT CCATCATTGC TTTTGCTCTC AGTTGTAAAG AATACCGGAA TGCCTTAGAG
    GAATTGTCCA AAGCCACTCA GCGGAACAGT GCTGAAGAAG GGCTTCCAAC AAACAGTACT
    TTAGACAGCA GACCAAAGAG TAGCAGCCCA ATTAGACTAC CTGAGATCAG TGGAGGACAG
    ACAAACCGTG CGGTAGAAGC AGAACCACAG CCAACCAAAA AGGCTTCTGG AATGTTGTCC
    TTCTTCCGAG GGACAGCAGG GAAAAGCCCT GACCTCTCTT CCCAGAAGAG AGAGACCTTA
    CGTGGAGCAG ATAGTGCTTA CTACCAAGTG GGGCAGACGG GCAAGGAGGG GACGGAGAAT
    CAAGGCATTG AGCCTCAAGA TGAAGTAGAT GGAGGAGATA CACAGAAGAA ACAACTTACA
    AATCCTCATG TGGAGCTTCA ATTTTCGGAT GCCAACGCCA AGTTTTACTG TCGGCTCTAC
    TATGCGGGAG AGTTTCATAA GATGCGAGAA GTGATTCTGG GCAGCAGCGA AGAAGATTTC
    ATTCGTTCCC TTTCTCACTC TTCTCCGTGG CAGGCCCGAG GAGGCAAATC AGGAGCTGCC
    TTTTACGCTA CTGAAGATGA CAGATTCATT TTGAAGCAAA TGCCTCGTCT GGAAGTCCAG
    TCTTTCCTTG ACTTCGCACC ACACTACTTC AATTACATTA CAAATGCCGT TCAGCAAAAG
    AGGCCTACTG CATTGGCCAA GATTCTTGGA GTTTACAGAA TTGGTTATAA GAATTCTCAG
    AACAACACTG AGAAGAAGCT AGATCTCCTT GTCATGGAAA ATCTTTTCTA CGGGAGAAAG
    ATGGCACAGG TTTTTGACTT GAAGGGTTCA CTTAGGAATC GAAATGTAAA AACCGATACC
    GGGAAAGAGA GCTGTGATGT TGTTCTTCTG GATGAAAATC TCTTAAAGAT GGTTCGAGAC
    AACCCTCTCT ATATCCGTTC TCATTCCAAA GCTGTGCTGA GAGCCTCGAT CCGGAGCGAC
    TCCCACTTCC TTTCTAGCCA CCTCATTATA GACTATTCTT TGCTAGTTGG GCGAGACGAT
    ACCAGCAATG AGCTAGTAGT TGGAATTATA GATTATATCC GAACATTTAC CTGGGACAAA
    AAGCTTGAGA TGGTTGTGAA ATCAACGGGA ATTTTAGGAG GACAAGGTAA AATGCCGACT
    GTGGTCTCTC CAGAGTTGTA CAGGACTAGA TTCTGTGAAG CCATGGACAA GTATTTCCTG
    ATGGTGCCAG ACCACTGGAC AGGCTTGGGT CTGAACTGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834422.1
    CDS155..6316
    Translation

    Target ORF information:

    RefSeq Version XM_024978654.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978654.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGAGGACAGT TTGCAACACC CCCAGGAGAA CTCAGACACC
    AGAAGGAAGG CAGAACCTAC TTTTGGAGGT CATGACCCTC GCACAGCTGT TCAACTCCGA
    AGCCTCAGCA CAGTATTAAA ACGCCTCAAG GAAATCATGG AGGGGAAGAG CCAGGATAGT
    GACCTGAAGC AATACTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGTAATGAG
    AAATTTACCA CTTTTAGGCG CAGACACCAC TGCCGACTCT GTGGGCAGAT TTTCTGCAGT
    CGTTGCTGCA ATCAAGAAAT CCCTGGAAAA TTTATGGGTT ATACAGGGGA CCTCCGAGCC
    TGTACATACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCCATT
    GGTGAAGACT TGAATGCTCT TTCAGACTCT GCTTCCTCTG TGTCTGTACT TGATCCAAAT
    GAACCCCGTA CACCTGTGGG GAGTAGAAAA GCCAGCCGCA ACATATTTTT AGAGGATGAT
    TTTGCCTGGC AAAGTTTAAT TCATCCAGAC TCCTCAAACA CTGCTCTTTC AACAAGACTT
    GTATCTGTTC AAGAGGATGC TGGGAAATCT CCTGCTCGAA ATAGATCAGC CAGCATTACT
    AATCTGTCAC TGGATCGATC TGCGTCTCCA ATGGTACCAT CATACGAGAC GTCTGTCAGT
    CCCCAGGCTA ACCGAACGTA TGTTCGGACA GAGACCACTG AGGATGAACG CAAAATTCTT
    CTGGACAGTG TTCAGTTAAA AGACCTATGG AAGAAAATCT GCCATCACAG CAGTGGAATG
    GAGTTTCAGG ATCACCGCTA CTGGCTGAGA ACACACCCCA ACTGCATTGT AGGAAAGGAG
    TTAGTCAACT GGTTAATCCG AAATGGACAC ATTGCTACAA GGGCACAGGC TATAGCAATT
    GGACAGGCAA TGGTGGATGG ACGTTGGCTG GATTGTGTCA GTCACCACGA CCAGCTCTTC
    AGGGATGAGT ATGCGCTGTA CAGGCCGCTG CAGAGTACAG AATTTTCCGA GACCCCTTCT
    CCGGACAGTG ACTCGGTGAA CTCTGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC
    ATCAAGTTTG ATGACAGTGA CACTGAGCAG ATAGCTGAAG AAGGTGACGA TAATTTGACT
    AAGTATTTGA TTTCTGACAA TGGAGGACAA CAGCTATCAA TAAGTGACGC CTTCATCAAA
    GAATCCTTAT TTAACCGTCG AGTTGAGGAA AAATCCAAAG AACTGCCTTT CACACCTTTG
    GGCTGGCATC ATAACAACCT GGAGCTGCTG AGGGAGGAGA ATGGAGAGAA ACAAGCTATG
    GAGAGGTTGC TTTCAGCTAA TCATAACCAC ATGATGGCGC TGCTCCAACA GTTGCTCCAT
    AGTGAGTCAT TGTCAACGTC TTGGAGGGAC ATCATCGTAT CCCTGGTCTG CCAGGTTGTT
    CAGACAGTCC GACCTGACGT CAAGAACCGG GATGATGACA TGGATATCCG CCAGTTTGTC
    CACATCAAAA AAATCCCAGG TGGAAAGAAA TTTGATTCTG TGGTTGTCAA TGGATTTGTT
    TGCACCAAGA ATATTGCACA TAAAAAGATG AATTCTTCTA TTAAAAACCC CAAAATTCTT
    CTGCTGAAAT GTTCCATTGA ATATCTCTAC AGAGAAGAAA CCAAATTTAC TTGCATTGAT
    CCTATTGTGC TTCAGGAAAG GGAATTCCTA AAGAATTATG TTCAGCGAAT AGTTGATGTT
    CGACCCACAC TGGTTCTGGT TGAGAAAACG GTATCTCGAA TTGCACAGGA TATGTTGCTG
    GAACACGGCA TTACTTTGGT CATTAATGTA AAATCACAAG TTTTGGAACG AGTTAGCCGG
    ATGACCCAAG GTGATTTGGT GATGTCAATG GACCAGCTGC TTACCAAACC CCACCTGGGC
    ACCTGTCACA AATTTTACAT GCAGATGTTT CAGTTGCCTA ATGAACAAAC CAAGACACTG
    ATGTTTTTTG AAGGCTGTCC ACAGCACCTG GGCTGTACAA TCAAGCTTAG AGGAGGCTCT
    GATTATGAGC TGGCTCGAGT TAAGGAGATC TTAATATTTA TGATCTGTGT AGCTTATCAT
    TCTCAACTAG AAATCTCCTT CCTTATGGAT GAATTTGCTA TGCCTCCTAC ATTAACGCAA
    AACCCTTCCT TCCATTCTCT GATTGAAGGA CAGGAGGATG AAGGGGCTTC ACAGGAGCCA
    TTCAGCGGAA GTCCCCTCCC CCGGGAGCCT GACTTCCCCC CAGACTTTCT GCCTTCTGAT
    GATGGCAGTT TGCTAGAAAC AAGGATTCTG TTTGAGAAGG GTGACCAGGA GAATAAGAGT
    GTGCCACAGG ATGTTGCCTC TTTGAAGCCT CAAGAGCATG CCCCAGCAGC TTGCCCGATG
    GGTGCCCCCT GTGCTCTCTT TCCATCAGTA CCAGAGTCAT TGCTTCCGCT CCATGTGGAT
    GACCAACAGG ACGCCATAGG CAGTGAGCAG CCAGAGGCTT TGCAGCAAAC AGAGGAGCTT
    CCGGATCCCA CAAGCCAGAT GAGAGCCTTT AGAGACCCTC TGCAGGATGA CACTGGCCTG
    TATGTTACCG AGGAAGTTAC CTCTTCTGAA GATAAACGGA AGACTGATTC TTTGACCTTT
    AAGCAAGAGT TGAAAGATGT GATCCTCTGT ATCTCGCCAG TCATCACATT CCGGGAGCCC
    TTCCTTTTAA CTGACAAGGG CATGAGATGC TCTACCCGAG ATTATTTTGC GGAGCAGGTT
    TACTGGTCTC CTCTCCTCAA TAAGGAGTTC AAGGAAATGG AGAGCAGGCG AAAGAAACAG
    ATGCTTAGGG ATCTCTCTGG CCTTCAGGGC ATGAATGGAA GTGTTCAGGC CAAGTCCATT
    CAAGTCCTGC CCTCACATGA GCTCGTGAGC ACCAGAATTG CTGAGCACCT GGGGGACAGC
    CAGAGCTTGG GGAGAATGCT AGCCGATTAT CGGGCCAGAG GAGGAAGAAT CCAGCAAAAA
    AACTCAGACC CTTTTGCTTA CTCAAAGGAG GCATCAAGCA CCTCAAGTGG AAAATCAGGA
    AGCAGAACTG AAGGTGATGA AGAAAAAGGG TTGATTACCA GTGATGCAGT GTGGTCGACA
    AAGGTGGACT GTCTGAACCC TGTCAACCAC CAGAGGCTGT GTGTGCTCTT CAGCAGCTCT
    TCTGCCCAGT CCAGCAACGC TCCCAGTGCC TGTGTCAGTC CTTGGATTGT AACAATGGAA
    TTTTATGGAA AAAATGATCT TACATTAGGA ATATTTTTAG AGAGATACTG TTTCCGGCCT
    TCCTATCAGT GCCCTAGCAT GTTCTGTGAT ACCCCCATGG TGCACCACAT TCGACGCTTT
    GTCCATGGCC AAGGCTGTGT GCAGATAATC CTAAAGGAGC TGGATTCTCC AGTACCTGGA
    TATCAGCATA CAATTCTCAC CTACTCCTGG TGCAGAATGT GCAAACAGGT AACACCAGTT
    GTTGCTCTTT CCAATGAGTC TTGGTCCATG TCGTTTGCAA AATACCTGGA ACTTAGGTTT
    TATGGGCACC AGTACACCCG CAGAGCCAAT GCTGAGCCAT GTGGTCACTC CATCCACCAC
    GATTATCACC AGTATTTCTC CTATAACCAG ATGGTGGCGT CTTTCAGTTA TTCTCCCATT
    CGGCTTCTTG AAGTGTGTGT TCCACTTCCC AAAATATTCA TTAAACGTCA AGTCCCACTA
    AAGGTGTCCC TTCTTCAGGA TCTGAAGGAC TTCTTTCAGA AAGTTTCACA GGTATATCTT
    GCTGTGGATG AAAGACTTGC GTCTTTGAAA ACTGATACAT TTAGCAAATC AAGAGAGGAA
    AAAATGGAAG ATATCTTTGC ACAAAAAGAG ATGGAAGAAA GTGAGTTCAA GAGCTGGACT
    GAGAAGATGC AGGCGCGGCT CCTGTCTTCC TCCATGGATA CCCCCCAGCA ACTGCAGTCA
    GTCTTCGAGT CCCTCATCGC CAAGAAACAA AGCCTCTGTG AGGTGCTGCA AGCTTGGAAT
    AACAGGCTGC AAGACCTTTT CCAACAAGAA AAGGGTAGGA AGCGGCCTTC AGTTCCTCCA
    AGTCCTGGAA GACTGAGACA AGGTGAAGAA AGCAAGATAA GTGCAGTGGA TGCATCTCCA
    CGGAATGCTT CTCCAGGACT TCCAAATGGA GAAAAAGAGG ACCGCTTCTT AGCCACCCTT
    TCCAGTCAGA GCTCCACCAG TTCCCCCCAT CTCCAGCTGC CTACTTCTCC TGAAGGCGTG
    CCTGAGCAGG TCATGGGAGG GCCCCCAGAT CTCGATACTG CCAGCAGTTC TGAAGATGTA
    TTTGATGGAC ATTTGCTGGG ATCTACAGAC AGCCAGGTGA AGGAAAAATC AACCATGAAA
    GCCATCTTTG CAAATTTGCT TCCAGGAAAT AGCTATAACC CTATTCCATT TCCTTTCGAT
    CCAGATAAAC ACTACTTAAT GTATGAACAT GAACGAGTGC CCATTGCAGT CTGTGAGAAG
    GAGCCCAGCT CCATCATTGC TTTTGCTCTC AGTTGTAAAG AATACCGGAA TGCCTTAGAG
    GAATTGTCCA AAGCCACTCA GCGGAACAGT GCTGAAGAAG GGCTTCCAAC AAACAGTACT
    TTAGACAGCA GACCAAAGAG TAGCAGCCCA ATTAGACTAC CTGAGATCAG TGGAGGACAG
    ACAAACCGTG CGGTAGAAGC AGAACCACAG CCAACCAAAA AGGCTTCTGG AATGTTGTCC
    TTCTTCCGAG GGACAGCAGG GAAAAGCCCT GACCTCTCTT CCCAGAAGAG AGAGACCTTA
    CGTGGAGCAG ATAGTGCTTA CTACCAAGTG GGGCAGACGG GCAAGGAGGG GACGGAGAAT
    CAAGGCATTG AGCCTCAAGA TGAAGTAGAT GGAGGAGATA CACAGAAGAA ACAACTTACA
    AATCCTCATG TGGAGCTTCA ATTTTCGGAT GCCAACGCCA AGTTTTACTG TCGGCTCTAC
    TATGCGGGAG AGTTTCATAA GATGCGAGAA GTGATTCTGG GCAGCAGCGA AGAAGATTTC
    ATTCGTTCCC TTTCTCACTC TTCTCCGTGG CAGGCCCGAG GAGGCAAATC AGGAGCTGCC
    TTTTACGCTA CTGAAGATGA CAGATTCATT TTGAAGCAAA TGCCTCGTCT GGAAGTCCAG
    TCTTTCCTTG ACTTCGCACC ACACTACTTC AATTACATTA CAAATGCCGT TCAGCAAAAG
    AGGCCTACTG CATTGGCCAA GATTCTTGGA GTTTACAGAA TTGGTTATAA GAATTCTCAG
    AACAACACTG AGAAGAAGCT AGATCTCCTT GTCATGGAAA ATCTTTTCTA CGGGAGAAAG
    ATGGCACAGG TTTTTGACTT GAAGGGTTCA CTTAGGAATC GAAATGTAAA AACCGATACC
    GGGAAAGAGA GCTGTGATGT TGTTCTTCTG GATGAAAATC TCTTAAAGAT GGTTCGAGAC
    AACCCTCTCT ATATCCGTTC TCATTCCAAA GCTGTGCTGA GAGCCTCGAT CCGGAGCGAC
    TCCCACTTCC TTTCTAGCCA CCTCATTATA GACTATTCTT TGCTAGTTGG GCGAGACGAT
    ACCAGCAATG AGCTAGTAGT TGGAATTATA GATTATATCC GAACATTTAC CTGGGACAAA
    AAGCTTGAGA TGGTTGTGAA ATCAACGGGA ATTTTAGGAG GACAAGGTAA AATGCCGACT
    GTGGTCTCTC CAGAGTTGTA CAGGACTAGA TTCTGTGAAG CCATGGACAA GTATTTCCTG
    ATGGTGCCAG ACCACTGGAC AGGCTTGGGT CTGAACTGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58666
    Clone ID Related Accession (Same CDS sequence) XM_024978654.1 , XM_010802277.2
    Accession Version XM_010802277.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6162bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802277.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGAGGACAGT TTGCAACACC CCCAGGAGAA CTCAGACACC
    AGAAGGAAGG CAGAACCTAC TTTTGGAGGT CATGACCCTC GCACAGCTGT TCAACTCCGA
    AGCCTCAGCA CAGTATTAAA ACGCCTCAAG GAAATCATGG AGGGGAAGAG CCAGGATAGT
    GACCTGAAGC AATACTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGTAATGAG
    AAATTTACCA CTTTTAGGCG CAGACACCAC TGCCGACTCT GTGGGCAGAT TTTCTGCAGT
    CGTTGCTGCA ATCAAGAAAT CCCTGGAAAA TTTATGGGTT ATACAGGGGA CCTCCGAGCC
    TGTACATACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCCATT
    GGTGAAGACT TGAATGCTCT TTCAGACTCT GCTTCCTCTG TGTCTGTACT TGATCCAAAT
    GAACCCCGTA CACCTGTGGG GAGTAGAAAA GCCAGCCGCA ACATATTTTT AGAGGATGAT
    TTTGCCTGGC AAAGTTTAAT TCATCCAGAC TCCTCAAACA CTGCTCTTTC AACAAGACTT
    GTATCTGTTC AAGAGGATGC TGGGAAATCT CCTGCTCGAA ATAGATCAGC CAGCATTACT
    AATCTGTCAC TGGATCGATC TGCGTCTCCA ATGGTACCAT CATACGAGAC GTCTGTCAGT
    CCCCAGGCTA ACCGAACGTA TGTTCGGACA GAGACCACTG AGGATGAACG CAAAATTCTT
    CTGGACAGTG TTCAGTTAAA AGACCTATGG AAGAAAATCT GCCATCACAG CAGTGGAATG
    GAGTTTCAGG ATCACCGCTA CTGGCTGAGA ACACACCCCA ACTGCATTGT AGGAAAGGAG
    TTAGTCAACT GGTTAATCCG AAATGGACAC ATTGCTACAA GGGCACAGGC TATAGCAATT
    GGACAGGCAA TGGTGGATGG ACGTTGGCTG GATTGTGTCA GTCACCACGA CCAGCTCTTC
    AGGGATGAGT ATGCGCTGTA CAGGCCGCTG CAGAGTACAG AATTTTCCGA GACCCCTTCT
    CCGGACAGTG ACTCGGTGAA CTCTGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC
    ATCAAGTTTG ATGACAGTGA CACTGAGCAG ATAGCTGAAG AAGGTGACGA TAATTTGACT
    AAGTATTTGA TTTCTGACAA TGGAGGACAA CAGCTATCAA TAAGTGACGC CTTCATCAAA
    GAATCCTTAT TTAACCGTCG AGTTGAGGAA AAATCCAAAG AACTGCCTTT CACACCTTTG
    GGCTGGCATC ATAACAACCT GGAGCTGCTG AGGGAGGAGA ATGGAGAGAA ACAAGCTATG
    GAGAGGTTGC TTTCAGCTAA TCATAACCAC ATGATGGCGC TGCTCCAACA GTTGCTCCAT
    AGTGAGTCAT TGTCAACGTC TTGGAGGGAC ATCATCGTAT CCCTGGTCTG CCAGGTTGTT
    CAGACAGTCC GACCTGACGT CAAGAACCGG GATGATGACA TGGATATCCG CCAGTTTGTC
    CACATCAAAA AAATCCCAGG TGGAAAGAAA TTTGATTCTG TGGTTGTCAA TGGATTTGTT
    TGCACCAAGA ATATTGCACA TAAAAAGATG AATTCTTCTA TTAAAAACCC CAAAATTCTT
    CTGCTGAAAT GTTCCATTGA ATATCTCTAC AGAGAAGAAA CCAAATTTAC TTGCATTGAT
    CCTATTGTGC TTCAGGAAAG GGAATTCCTA AAGAATTATG TTCAGCGAAT AGTTGATGTT
    CGACCCACAC TGGTTCTGGT TGAGAAAACG GTATCTCGAA TTGCACAGGA TATGTTGCTG
    GAACACGGCA TTACTTTGGT CATTAATGTA AAATCACAAG TTTTGGAACG AGTTAGCCGG
    ATGACCCAAG GTGATTTGGT GATGTCAATG GACCAGCTGC TTACCAAACC CCACCTGGGC
    ACCTGTCACA AATTTTACAT GCAGATGTTT CAGTTGCCTA ATGAACAAAC CAAGACACTG
    ATGTTTTTTG AAGGCTGTCC ACAGCACCTG GGCTGTACAA TCAAGCTTAG AGGAGGCTCT
    GATTATGAGC TGGCTCGAGT TAAGGAGATC TTAATATTTA TGATCTGTGT AGCTTATCAT
    TCTCAACTAG AAATCTCCTT CCTTATGGAT GAATTTGCTA TGCCTCCTAC ATTAACGCAA
    AACCCTTCCT TCCATTCTCT GATTGAAGGA CAGGAGGATG AAGGGGCTTC ACAGGAGCCA
    TTCAGCGGAA GTCCCCTCCC CCGGGAGCCT GACTTCCCCC CAGACTTTCT GCCTTCTGAT
    GATGGCAGTT TGCTAGAAAC AAGGATTCTG TTTGAGAAGG GTGACCAGGA GAATAAGAGT
    GTGCCACAGG ATGTTGCCTC TTTGAAGCCT CAAGAGCATG CCCCAGCAGC TTGCCCGATG
    GGTGCCCCCT GTGCTCTCTT TCCATCAGTA CCAGAGTCAT TGCTTCCGCT CCATGTGGAT
    GACCAACAGG ACGCCATAGG CAGTGAGCAG CCAGAGGCTT TGCAGCAAAC AGAGGAGCTT
    CCGGATCCCA CAAGCCAGAT GAGAGCCTTT AGAGACCCTC TGCAGGATGA CACTGGCCTG
    TATGTTACCG AGGAAGTTAC CTCTTCTGAA GATAAACGGA AGACTGATTC TTTGACCTTT
    AAGCAAGAGT TGAAAGATGT GATCCTCTGT ATCTCGCCAG TCATCACATT CCGGGAGCCC
    TTCCTTTTAA CTGACAAGGG CATGAGATGC TCTACCCGAG ATTATTTTGC GGAGCAGGTT
    TACTGGTCTC CTCTCCTCAA TAAGGAGTTC AAGGAAATGG AGAGCAGGCG AAAGAAACAG
    ATGCTTAGGG ATCTCTCTGG CCTTCAGGGC ATGAATGGAA GTGTTCAGGC CAAGTCCATT
    CAAGTCCTGC CCTCACATGA GCTCGTGAGC ACCAGAATTG CTGAGCACCT GGGGGACAGC
    CAGAGCTTGG GGAGAATGCT AGCCGATTAT CGGGCCAGAG GAGGAAGAAT CCAGCAAAAA
    AACTCAGACC CTTTTGCTTA CTCAAAGGAG GCATCAAGCA CCTCAAGTGG AAAATCAGGA
    AGCAGAACTG AAGGTGATGA AGAAAAAGGG TTGATTACCA GTGATGCAGT GTGGTCGACA
    AAGGTGGACT GTCTGAACCC TGTCAACCAC CAGAGGCTGT GTGTGCTCTT CAGCAGCTCT
    TCTGCCCAGT CCAGCAACGC TCCCAGTGCC TGTGTCAGTC CTTGGATTGT AACAATGGAA
    TTTTATGGAA AAAATGATCT TACATTAGGA ATATTTTTAG AGAGATACTG TTTCCGGCCT
    TCCTATCAGT GCCCTAGCAT GTTCTGTGAT ACCCCCATGG TGCACCACAT TCGACGCTTT
    GTCCATGGCC AAGGCTGTGT GCAGATAATC CTAAAGGAGC TGGATTCTCC AGTACCTGGA
    TATCAGCATA CAATTCTCAC CTACTCCTGG TGCAGAATGT GCAAACAGGT AACACCAGTT
    GTTGCTCTTT CCAATGAGTC TTGGTCCATG TCGTTTGCAA AATACCTGGA ACTTAGGTTT
    TATGGGCACC AGTACACCCG CAGAGCCAAT GCTGAGCCAT GTGGTCACTC CATCCACCAC
    GATTATCACC AGTATTTCTC CTATAACCAG ATGGTGGCGT CTTTCAGTTA TTCTCCCATT
    CGGCTTCTTG AAGTGTGTGT TCCACTTCCC AAAATATTCA TTAAACGTCA AGTCCCACTA
    AAGGTGTCCC TTCTTCAGGA TCTGAAGGAC TTCTTTCAGA AAGTTTCACA GGTATATCTT
    GCTGTGGATG AAAGACTTGC GTCTTTGAAA ACTGATACAT TTAGCAAATC AAGAGAGGAA
    AAAATGGAAG ATATCTTTGC ACAAAAAGAG ATGGAAGAAA GTGAGTTCAA GAGCTGGACT
    GAGAAGATGC AGGCGCGGCT CCTGTCTTCC TCCATGGATA CCCCCCAGCA ACTGCAGTCA
    GTCTTCGAGT CCCTCATCGC CAAGAAACAA AGCCTCTGTG AGGTGCTGCA AGCTTGGAAT
    AACAGGCTGC AAGACCTTTT CCAACAAGAA AAGGGTAGGA AGCGGCCTTC AGTTCCTCCA
    AGTCCTGGAA GACTGAGACA AGGTGAAGAA AGCAAGATAA GTGCAGTGGA TGCATCTCCA
    CGGAATGCTT CTCCAGGACT TCCAAATGGA GAAAAAGAGG ACCGCTTCTT AGCCACCCTT
    TCCAGTCAGA GCTCCACCAG TTCCCCCCAT CTCCAGCTGC CTACTTCTCC TGAAGGCGTG
    CCTGAGCAGG TCATGGGAGG GCCCCCAGAT CTCGATACTG CCAGCAGTTC TGAAGATGTA
    TTTGATGGAC ATTTGCTGGG ATCTACAGAC AGCCAGGTGA AGGAAAAATC AACCATGAAA
    GCCATCTTTG CAAATTTGCT TCCAGGAAAT AGCTATAACC CTATTCCATT TCCTTTCGAT
    CCAGATAAAC ACTACTTAAT GTATGAACAT GAACGAGTGC CCATTGCAGT CTGTGAGAAG
    GAGCCCAGCT CCATCATTGC TTTTGCTCTC AGTTGTAAAG AATACCGGAA TGCCTTAGAG
    GAATTGTCCA AAGCCACTCA GCGGAACAGT GCTGAAGAAG GGCTTCCAAC AAACAGTACT
    TTAGACAGCA GACCAAAGAG TAGCAGCCCA ATTAGACTAC CTGAGATCAG TGGAGGACAG
    ACAAACCGTG CGGTAGAAGC AGAACCACAG CCAACCAAAA AGGCTTCTGG AATGTTGTCC
    TTCTTCCGAG GGACAGCAGG GAAAAGCCCT GACCTCTCTT CCCAGAAGAG AGAGACCTTA
    CGTGGAGCAG ATAGTGCTTA CTACCAAGTG GGGCAGACGG GCAAGGAGGG GACGGAGAAT
    CAAGGCATTG AGCCTCAAGA TGAAGTAGAT GGAGGAGATA CACAGAAGAA ACAACTTACA
    AATCCTCATG TGGAGCTTCA ATTTTCGGAT GCCAACGCCA AGTTTTACTG TCGGCTCTAC
    TATGCGGGAG AGTTTCATAA GATGCGAGAA GTGATTCTGG GCAGCAGCGA AGAAGATTTC
    ATTCGTTCCC TTTCTCACTC TTCTCCGTGG CAGGCCCGAG GAGGCAAATC AGGAGCTGCC
    TTTTACGCTA CTGAAGATGA CAGATTCATT TTGAAGCAAA TGCCTCGTCT GGAAGTCCAG
    TCTTTCCTTG ACTTCGCACC ACACTACTTC AATTACATTA CAAATGCCGT TCAGCAAAAG
    AGGCCTACTG CATTGGCCAA GATTCTTGGA GTTTACAGAA TTGGTTATAA GAATTCTCAG
    AACAACACTG AGAAGAAGCT AGATCTCCTT GTCATGGAAA ATCTTTTCTA CGGGAGAAAG
    ATGGCACAGG TTTTTGACTT GAAGGGTTCA CTTAGGAATC GAAATGTAAA AACCGATACC
    GGGAAAGAGA GCTGTGATGT TGTTCTTCTG GATGAAAATC TCTTAAAGAT GGTTCGAGAC
    AACCCTCTCT ATATCCGTTC TCATTCCAAA GCTGTGCTGA GAGCCTCGAT CCGGAGCGAC
    TCCCACTTCC TTTCTAGCCA CCTCATTATA GACTATTCTT TGCTAGTTGG GCGAGACGAT
    ACCAGCAATG AGCTAGTAGT TGGAATTATA GATTATATCC GAACATTTAC CTGGGACAAA
    AAGCTTGAGA TGGTTGTGAA ATCAACGGGA ATTTTAGGAG GACAAGGTAA AATGCCGACT
    GTGGTCTCTC CAGAGTTGTA CAGGACTAGA TTCTGTGAAG CCATGGACAA GTATTTCCTG
    ATGGTGCCAG ACCACTGGAC AGGCTTGGGT CTGAACTGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800579.1
    CDS132..6293
    Translation

    Target ORF information:

    RefSeq Version XM_010802277.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802277.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCACAG ATGATAAGAC TTCCCCAACA TTGGACTCCG CTAATGACTT GCCTCGGTCT 
    CCTACCAGTC CTTCTCATCT CACACACTTT AAACCTCTGA CTCCTGATCA GGACGAGCCT
    CCTTTTAAAT CAGCGTATAG TTCTTTTGTC AATCTCTTTC GATTTAACAA AGAGAGAGGA
    GAAGGGGGCC AGGGAGAGCA ACAGTCTCTG AGTGGAAGTT GGACCAGCCC TCAGCTCCCT
    TCAAGGACAC AATCTGTGAG ATCACCTACA CCTTATAAAA AGCGGCTTAA TGAGGAGTTC
    CAGCGTCGAT CTTCAGTGTT AGAGGACAGT TTGCAACACC CCCAGGAGAA CTCAGACACC
    AGAAGGAAGG CAGAACCTAC TTTTGGAGGT CATGACCCTC GCACAGCTGT TCAACTCCGA
    AGCCTCAGCA CAGTATTAAA ACGCCTCAAG GAAATCATGG AGGGGAAGAG CCAGGATAGT
    GACCTGAAGC AATACTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGTAATGAG
    AAATTTACCA CTTTTAGGCG CAGACACCAC TGCCGACTCT GTGGGCAGAT TTTCTGCAGT
    CGTTGCTGCA ATCAAGAAAT CCCTGGAAAA TTTATGGGTT ATACAGGGGA CCTCCGAGCC
    TGTACATACT GTAGAAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCCATT
    GGTGAAGACT TGAATGCTCT TTCAGACTCT GCTTCCTCTG TGTCTGTACT TGATCCAAAT
    GAACCCCGTA CACCTGTGGG GAGTAGAAAA GCCAGCCGCA ACATATTTTT AGAGGATGAT
    TTTGCCTGGC AAAGTTTAAT TCATCCAGAC TCCTCAAACA CTGCTCTTTC AACAAGACTT
    GTATCTGTTC AAGAGGATGC TGGGAAATCT CCTGCTCGAA ATAGATCAGC CAGCATTACT
    AATCTGTCAC TGGATCGATC TGCGTCTCCA ATGGTACCAT CATACGAGAC GTCTGTCAGT
    CCCCAGGCTA ACCGAACGTA TGTTCGGACA GAGACCACTG AGGATGAACG CAAAATTCTT
    CTGGACAGTG TTCAGTTAAA AGACCTATGG AAGAAAATCT GCCATCACAG CAGTGGAATG
    GAGTTTCAGG ATCACCGCTA CTGGCTGAGA ACACACCCCA ACTGCATTGT AGGAAAGGAG
    TTAGTCAACT GGTTAATCCG AAATGGACAC ATTGCTACAA GGGCACAGGC TATAGCAATT
    GGACAGGCAA TGGTGGATGG ACGTTGGCTG GATTGTGTCA GTCACCACGA CCAGCTCTTC
    AGGGATGAGT ATGCGCTGTA CAGGCCGCTG CAGAGTACAG AATTTTCCGA GACCCCTTCT
    CCGGACAGTG ACTCGGTGAA CTCTGTGGAA GGACACTCTG AGCCATCCTG GTTTAAAGAC
    ATCAAGTTTG ATGACAGTGA CACTGAGCAG ATAGCTGAAG AAGGTGACGA TAATTTGACT
    AAGTATTTGA TTTCTGACAA TGGAGGACAA CAGCTATCAA TAAGTGACGC CTTCATCAAA
    GAATCCTTAT TTAACCGTCG AGTTGAGGAA AAATCCAAAG AACTGCCTTT CACACCTTTG
    GGCTGGCATC ATAACAACCT GGAGCTGCTG AGGGAGGAGA ATGGAGAGAA ACAAGCTATG
    GAGAGGTTGC TTTCAGCTAA TCATAACCAC ATGATGGCGC TGCTCCAACA GTTGCTCCAT
    AGTGAGTCAT TGTCAACGTC TTGGAGGGAC ATCATCGTAT CCCTGGTCTG CCAGGTTGTT
    CAGACAGTCC GACCTGACGT CAAGAACCGG GATGATGACA TGGATATCCG CCAGTTTGTC
    CACATCAAAA AAATCCCAGG TGGAAAGAAA TTTGATTCTG TGGTTGTCAA TGGATTTGTT
    TGCACCAAGA ATATTGCACA TAAAAAGATG AATTCTTCTA TTAAAAACCC CAAAATTCTT
    CTGCTGAAAT GTTCCATTGA ATATCTCTAC AGAGAAGAAA CCAAATTTAC TTGCATTGAT
    CCTATTGTGC TTCAGGAAAG GGAATTCCTA AAGAATTATG TTCAGCGAAT AGTTGATGTT
    CGACCCACAC TGGTTCTGGT TGAGAAAACG GTATCTCGAA TTGCACAGGA TATGTTGCTG
    GAACACGGCA TTACTTTGGT CATTAATGTA AAATCACAAG TTTTGGAACG AGTTAGCCGG
    ATGACCCAAG GTGATTTGGT GATGTCAATG GACCAGCTGC TTACCAAACC CCACCTGGGC
    ACCTGTCACA AATTTTACAT GCAGATGTTT CAGTTGCCTA ATGAACAAAC CAAGACACTG
    ATGTTTTTTG AAGGCTGTCC ACAGCACCTG GGCTGTACAA TCAAGCTTAG AGGAGGCTCT
    GATTATGAGC TGGCTCGAGT TAAGGAGATC TTAATATTTA TGATCTGTGT AGCTTATCAT
    TCTCAACTAG AAATCTCCTT CCTTATGGAT GAATTTGCTA TGCCTCCTAC ATTAACGCAA
    AACCCTTCCT TCCATTCTCT GATTGAAGGA CAGGAGGATG AAGGGGCTTC ACAGGAGCCA
    TTCAGCGGAA GTCCCCTCCC CCGGGAGCCT GACTTCCCCC CAGACTTTCT GCCTTCTGAT
    GATGGCAGTT TGCTAGAAAC AAGGATTCTG TTTGAGAAGG GTGACCAGGA GAATAAGAGT
    GTGCCACAGG ATGTTGCCTC TTTGAAGCCT CAAGAGCATG CCCCAGCAGC TTGCCCGATG
    GGTGCCCCCT GTGCTCTCTT TCCATCAGTA CCAGAGTCAT TGCTTCCGCT CCATGTGGAT
    GACCAACAGG ACGCCATAGG CAGTGAGCAG CCAGAGGCTT TGCAGCAAAC AGAGGAGCTT
    CCGGATCCCA CAAGCCAGAT GAGAGCCTTT AGAGACCCTC TGCAGGATGA CACTGGCCTG
    TATGTTACCG AGGAAGTTAC CTCTTCTGAA GATAAACGGA AGACTGATTC TTTGACCTTT
    AAGCAAGAGT TGAAAGATGT GATCCTCTGT ATCTCGCCAG TCATCACATT CCGGGAGCCC
    TTCCTTTTAA CTGACAAGGG CATGAGATGC TCTACCCGAG ATTATTTTGC GGAGCAGGTT
    TACTGGTCTC CTCTCCTCAA TAAGGAGTTC AAGGAAATGG AGAGCAGGCG AAAGAAACAG
    ATGCTTAGGG ATCTCTCTGG CCTTCAGGGC ATGAATGGAA GTGTTCAGGC CAAGTCCATT
    CAAGTCCTGC CCTCACATGA GCTCGTGAGC ACCAGAATTG CTGAGCACCT GGGGGACAGC
    CAGAGCTTGG GGAGAATGCT AGCCGATTAT CGGGCCAGAG GAGGAAGAAT CCAGCAAAAA
    AACTCAGACC CTTTTGCTTA CTCAAAGGAG GCATCAAGCA CCTCAAGTGG AAAATCAGGA
    AGCAGAACTG AAGGTGATGA AGAAAAAGGG TTGATTACCA GTGATGCAGT GTGGTCGACA
    AAGGTGGACT GTCTGAACCC TGTCAACCAC CAGAGGCTGT GTGTGCTCTT CAGCAGCTCT
    TCTGCCCAGT CCAGCAACGC TCCCAGTGCC TGTGTCAGTC CTTGGATTGT AACAATGGAA
    TTTTATGGAA AAAATGATCT TACATTAGGA ATATTTTTAG AGAGATACTG TTTCCGGCCT
    TCCTATCAGT GCCCTAGCAT GTTCTGTGAT ACCCCCATGG TGCACCACAT TCGACGCTTT
    GTCCATGGCC AAGGCTGTGT GCAGATAATC CTAAAGGAGC TGGATTCTCC AGTACCTGGA
    TATCAGCATA CAATTCTCAC CTACTCCTGG TGCAGAATGT GCAAACAGGT AACACCAGTT
    GTTGCTCTTT CCAATGAGTC TTGGTCCATG TCGTTTGCAA AATACCTGGA ACTTAGGTTT
    TATGGGCACC AGTACACCCG CAGAGCCAAT GCTGAGCCAT GTGGTCACTC CATCCACCAC
    GATTATCACC AGTATTTCTC CTATAACCAG ATGGTGGCGT CTTTCAGTTA TTCTCCCATT
    CGGCTTCTTG AAGTGTGTGT TCCACTTCCC AAAATATTCA TTAAACGTCA AGTCCCACTA
    AAGGTGTCCC TTCTTCAGGA TCTGAAGGAC TTCTTTCAGA AAGTTTCACA GGTATATCTT
    GCTGTGGATG AAAGACTTGC GTCTTTGAAA ACTGATACAT TTAGCAAATC AAGAGAGGAA
    AAAATGGAAG ATATCTTTGC ACAAAAAGAG ATGGAAGAAA GTGAGTTCAA GAGCTGGACT
    GAGAAGATGC AGGCGCGGCT CCTGTCTTCC TCCATGGATA CCCCCCAGCA ACTGCAGTCA
    GTCTTCGAGT CCCTCATCGC CAAGAAACAA AGCCTCTGTG AGGTGCTGCA AGCTTGGAAT
    AACAGGCTGC AAGACCTTTT CCAACAAGAA AAGGGTAGGA AGCGGCCTTC AGTTCCTCCA
    AGTCCTGGAA GACTGAGACA AGGTGAAGAA AGCAAGATAA GTGCAGTGGA TGCATCTCCA
    CGGAATGCTT CTCCAGGACT TCCAAATGGA GAAAAAGAGG ACCGCTTCTT AGCCACCCTT
    TCCAGTCAGA GCTCCACCAG TTCCCCCCAT CTCCAGCTGC CTACTTCTCC TGAAGGCGTG
    CCTGAGCAGG TCATGGGAGG GCCCCCAGAT CTCGATACTG CCAGCAGTTC TGAAGATGTA
    TTTGATGGAC ATTTGCTGGG ATCTACAGAC AGCCAGGTGA AGGAAAAATC AACCATGAAA
    GCCATCTTTG CAAATTTGCT TCCAGGAAAT AGCTATAACC CTATTCCATT TCCTTTCGAT
    CCAGATAAAC ACTACTTAAT GTATGAACAT GAACGAGTGC CCATTGCAGT CTGTGAGAAG
    GAGCCCAGCT CCATCATTGC TTTTGCTCTC AGTTGTAAAG AATACCGGAA TGCCTTAGAG
    GAATTGTCCA AAGCCACTCA GCGGAACAGT GCTGAAGAAG GGCTTCCAAC AAACAGTACT
    TTAGACAGCA GACCAAAGAG TAGCAGCCCA ATTAGACTAC CTGAGATCAG TGGAGGACAG
    ACAAACCGTG CGGTAGAAGC AGAACCACAG CCAACCAAAA AGGCTTCTGG AATGTTGTCC
    TTCTTCCGAG GGACAGCAGG GAAAAGCCCT GACCTCTCTT CCCAGAAGAG AGAGACCTTA
    CGTGGAGCAG ATAGTGCTTA CTACCAAGTG GGGCAGACGG GCAAGGAGGG GACGGAGAAT
    CAAGGCATTG AGCCTCAAGA TGAAGTAGAT GGAGGAGATA CACAGAAGAA ACAACTTACA
    AATCCTCATG TGGAGCTTCA ATTTTCGGAT GCCAACGCCA AGTTTTACTG TCGGCTCTAC
    TATGCGGGAG AGTTTCATAA GATGCGAGAA GTGATTCTGG GCAGCAGCGA AGAAGATTTC
    ATTCGTTCCC TTTCTCACTC TTCTCCGTGG CAGGCCCGAG GAGGCAAATC AGGAGCTGCC
    TTTTACGCTA CTGAAGATGA CAGATTCATT TTGAAGCAAA TGCCTCGTCT GGAAGTCCAG
    TCTTTCCTTG ACTTCGCACC ACACTACTTC AATTACATTA CAAATGCCGT TCAGCAAAAG
    AGGCCTACTG CATTGGCCAA GATTCTTGGA GTTTACAGAA TTGGTTATAA GAATTCTCAG
    AACAACACTG AGAAGAAGCT AGATCTCCTT GTCATGGAAA ATCTTTTCTA CGGGAGAAAG
    ATGGCACAGG TTTTTGACTT GAAGGGTTCA CTTAGGAATC GAAATGTAAA AACCGATACC
    GGGAAAGAGA GCTGTGATGT TGTTCTTCTG GATGAAAATC TCTTAAAGAT GGTTCGAGAC
    AACCCTCTCT ATATCCGTTC TCATTCCAAA GCTGTGCTGA GAGCCTCGAT CCGGAGCGAC
    TCCCACTTCC TTTCTAGCCA CCTCATTATA GACTATTCTT TGCTAGTTGG GCGAGACGAT
    ACCAGCAATG AGCTAGTAGT TGGAATTATA GATTATATCC GAACATTTAC CTGGGACAAA
    AAGCTTGAGA TGGTTGTGAA ATCAACGGGA ATTTTAGGAG GACAAGGTAA AATGCCGACT
    GTGGTCTCTC CAGAGTTGTA CAGGACTAGA TTCTGTGAAG CCATGGACAA GTATTTCCTG
    ATGGTGCCAG ACCACTGGAC AGGCTTGGGT CTGAACTGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.