DMWD cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DMWD
Entrez Gene ID	790091
Full Name	DM1 locus, WD repeat containing
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002695097.3	XP_002695143.1	dystrophia myotonica WD repeat-containing protein isoform X1
XM_010823770.2	XP_010822072.2	dystrophia myotonica WD repeat-containing protein isoform X1
XM_005219442.3	XP_005219499.1	dystrophia myotonica WD repeat-containing protein isoform X2
XM_005219442.2	XP_005219499.1	dystrophia myotonica WD repeat-containing protein isoform X2
XM_015467596.1	XP_015323082.1	dystrophia myotonica WD repeat-containing protein isoform X2
XM_002695097.4	XP_002695143.1	dystrophia myotonica WD repeat-containing protein isoform X1
XM_024979159.1	XP_024834927.1	dystrophia myotonica WD repeat-containing protein isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	DMWD	NP_004934.1
Pan troglodytes (chimpanzee)	DMWD	XP_001166545.1
Danio rerio (zebrafish)	LOC571996	XP_700740.5
Rattus norvegicus (Norway rat)	Dmwd	XP_006228596.1
Mus musculus (house mouse)	Dmwd	NP_034188.2
Canis lupus familiaris (dog)	DMWD	XP_541550.3
Bos taurus (cattle)	DMWD	XP_002695143.1

Gene Ontology

The following DMWD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DMWD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa225642	XM_024979159.1 Latest version!	Bos taurus DM1 locus, WD repeat containing (DMWD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa29011	XM_010823770.2 Latest version!	Bos taurus dystrophia myotonica, WD repeat containing (DMWD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa29011	XM_002695097.4 Latest version!	Bos taurus DM1 locus, WD repeat containing (DMWD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa29012	XM_005219442.3 Latest version!	Bos taurus DM1 locus, WD repeat containing (DMWD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OBa29012	XM_015467596.1 Latest version!	Bos taurus dystrophia myotonica, WD repeat containing (DMWD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
View Old Accession Versions >>
OBa29012	XM_005219442.2	Bos taurus dystrophia myotonica, WD repeat containing (DMWD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OBa29011	XM_002695097.3	Bos taurus dystrophia myotonica, WD repeat containing (DMWD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DMWD ( XM_024979159.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834927.1 Bos taurus DM1 locus, WD repeat containing (DMWD), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa225642
Clone ID Related Accession (Same CDS sequence)	XM_024979159.1
Accession Version	XM_024979159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystrophia myotonica WD repeat-containing protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGC GGGGGCTCAT GAAGAGCTAC TTTGGGGGCC TGCTCTGTGT GTGCTGGAGC 
CCCGACGGGC GCTACGTCGT GACGGGGGGC GAAGACGACC TGGTCACCGT GTGGTCCTTC 
ACCGAGGGCC GCGTGGTGGC CCGGGGCCAC GGCCACAAGT CCTGGGTCAA CGCCGTGGCC 
TTCGACCCCT ACACCACGAG GGCGGAGGAG GCAGCGGCCG CGGGCGCTGA CGAGGAGCGG 
AGTGGCGAGG AGGAGGAGGA GCCGGAGGCG GGGAGCACAG GCTCGGGCGG GGGCGCCCCC 
CTCTCCCCGC TGCCCAAGGC CGGCTCCATC ACGTACCGCT TCGGCTCAGC CGGCCAGGAC 
ACGCAGTTCT GCCTGTGGGA CCTCACGGAA GACGTGCTCT ACCCACACCC TCCCCTGGCC 
CGCACGCGCA CCCTCCCAGG CACGCCTGGC ACCACGCCGC CTACCGCCAG CGGCTCGCGG 
GGCGGCGAGC CTGGCCCCGG GCCCCTGCCC CGTTCACTGT CCCGCTCCAA CAGCCTTCCG 
CACCCCGCGG GCAGCGGCAA GGCGGGTGGC CCGGGCACGG CAGCAGAGCC TGGCACGCCC 
TTCAGCATCG GCCGCTTTGC CACGCTCACG CTGCAGGAGC GGCGGGACCG GGGGGCCGAG 
AAAGAGCACA AGCGCTACCA CAGTCTGGGC AACATCAGCC GGGGTGGCAG CGGGGGCGGG 
GGCGGCGGGG ACAAGCCCAG CGGTGCCGCC CCCCGAAGCC GGCTGGACCC CGCCAAGGTG 
CTGGGCACCG CGCTTTGCCC GCGCATCCAC GAGGTACCGC TGCTGGAGCC ACTGGTATGC 
AAGAAGATCG CCCAGGAGCG GCTCACGGTC CTCCTCTTCC TGGAGGACTG CATCATCACC 
GCCTGCCAGG AGGGCCTCAT CTGCACCTGG GCCCGGCCGG GCAAGGCGTT CACAGATGAG 
GAGACCGAGG CCCAGACAGG GGAAGGAAGT TGGCCCAGGT CACCCAGCAA GTCAGTGGTA 
GAGGGCATCT CCTCCCAGCC AGGCAACTCC CCAAGCGGCA CAGTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834927.1
CDS	655..1725
Translation	MLLRGLMKSYFGGLLCVCWSPDGRYVVTGGEDDLVTVWSFTEGRVVARGHGHKSWVNAVAFDPYTTRAEEAAAAGADEERSGEEEEEPEAGSTGSGGGAPLSPLPKAGSITYRFGSAGQDTQFCLWDLTEDVLYPHPPLARTRTLPGTPGTTPPTASGSRGGEPGPGPLPRSLSRSNSLPHPAGSGKAGGPGTAAEPGTPFSIGRFATLTLQERRDRGAEKEHKRYHSLGNISRGGSGGGGGGDKPSGAAPRSRLDPAKVLGTALCPRIHEVPLLEPLVCKKIAQERLTVLLFLEDCIITACQEGLICTWARPGKAFTDEETEAQTGEGSWPRSPSKSVVEGISSQPGNSPSGTVV

Target ORF information:

RefSeq Version	XM_024979159.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DM1 locus, WD repeat containing (DMWD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979159.1

ORF Insert Sequence:

ATGCTCCTGC GGGGGCTCAT GAAGAGCTAC TTTGGGGGCC TGCTCTGTGT GTGCTGGAGC 
CCCGACGGGC GCTACGTCGT GACGGGGGGC GAAGACGACC TGGTCACCGT GTGGTCCTTC 
ACCGAGGGCC GCGTGGTGGC CCGGGGCCAC GGCCACAAGT CCTGGGTCAA CGCCGTGGCC 
TTCGACCCCT ACACCACGAG GGCGGAGGAG GCAGCGGCCG CGGGCGCTGA CGAGGAGCGG 
AGTGGCGAGG AGGAGGAGGA GCCGGAGGCG GGGAGCACAG GCTCGGGCGG GGGCGCCCCC 
CTCTCCCCGC TGCCCAAGGC CGGCTCCATC ACGTACCGCT TCGGCTCAGC CGGCCAGGAC 
ACGCAGTTCT GCCTGTGGGA CCTCACGGAA GACGTGCTCT ACCCACACCC TCCCCTGGCC 
CGCACGCGCA CCCTCCCAGG CACGCCTGGC ACCACGCCGC CTACCGCCAG CGGCTCGCGG 
GGCGGCGAGC CTGGCCCCGG GCCCCTGCCC CGTTCACTGT CCCGCTCCAA CAGCCTTCCG 
CACCCCGCGG GCAGCGGCAA GGCGGGTGGC CCGGGCACGG CAGCAGAGCC TGGCACGCCC 
TTCAGCATCG GCCGCTTTGC CACGCTCACG CTGCAGGAGC GGCGGGACCG GGGGGCCGAG 
AAAGAGCACA AGCGCTACCA CAGTCTGGGC AACATCAGCC GGGGTGGCAG CGGGGGCGGG 
GGCGGCGGGG ACAAGCCCAG CGGTGCCGCC CCCCGAAGCC GGCTGGACCC CGCCAAGGTG 
CTGGGCACCG CGCTTTGCCC GCGCATCCAC GAGGTACCGC TGCTGGAGCC ACTGGTATGC 
AAGAAGATCG CCCAGGAGCG GCTCACGGTC CTCCTCTTCC TGGAGGACTG CATCATCACC 
GCCTGCCAGG AGGGCCTCAT CTGCACCTGG GCCCGGCCGG GCAAGGCGTT CACAGATGAG 
GAGACCGAGG CCCAGACAGG GGAAGGAAGT TGGCCCAGGT CACCCAGCAA GTCAGTGGTA 
GAGGGCATCT CCTCCCAGCC AGGCAACTCC CCAAGCGGCA CAGTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMWD ( XM_002695097.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002695143.1 Bos taurus dystrophia myotonica, WD repeat containing (DMWD), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa29011
Clone ID Related Accession (Same CDS sequence)	XM_002695097.3 , XM_010823770.2 , XM_002695097.4
Accession Version	XM_002695097.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dystrophia myotonica WD repeat-containing protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_002695097.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GCGGAGCGGA GGGCGGCTCG GGCCCCGGCG CCGCCATGGG GGATTGCGCG 
GAAATCAAGT CGCAGTTCCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGGC 
GCCGCCCGCA GATCGGGTCC GGCTTCCGCC CAGACCCCGG CGCCGCCCCA GCCGCCGCAG 
CCCCCGCCCG GCCCTGCCTC TGCCTCGGGC CCAGGCGCCG CGGGCCCCGC TCCGTCCCCT 
CCGCCTGCAG GCCCCGGGCC CGGGCCCGCG CTGCCTGCGG TGCGCCTAAG CCTCGTGCGT 
CTCGGGGAGC CCGACAGCGC CGGGGCCGGG GAGCCGCCTG CCACGCCCGC GGGACTGGGC 
GCCGGGGGAG ACCGCGTCTG CTTCAACTTG GGCCGCGAAC TCTATTTCTA CCCGGGCTGC 
TGTCGCCGTG GGAGCCAACG GTCCATTGAC CTCAACAAGC CAATTGACAA GCGGATCTAT 
AAGGGCACTC AGCCCACCTG CCATGACTTC AACCAGTTCA CTGCTGCCAC GGAGACCATC 
TCCCTGCTGG TGGGCTTCTC AGCCGGTCAA GTCCAGTACC TGGATCTCAT CAAGAAGGAC 
ACCAGCAAAC TATTCAATGA GGAGCGGCTC ATTGACAAGA CCAAGGTGAC ATATCTGAAG 
TGGCTGCCCG AGTCAGAGAG CCTGTTCCTG GCGTCCCACG CCAGCGGCCA CCTGTACCTG 
TACAACGTCG GCCACCCGTG CGCCTCGGCG CCGCCCCAGT ATAGCCTGCT GAAGCAGGGC 
GAGGGCTTCG CCGTCTACGC CGCCAAGAGC AAGGCGCCCC GCAACCCGCT GGCCAAGTGG 
GCGGTGGGCG AGGGGCCCCT CAACGAGTTC GCCTTCTCGC CCGACGGCCG GCACCTGGCC 
TGCGTCAGCC AGGATGGCTG CCTGCGCGTC TTCCACTTCG ATTCCATGCT CCTGCGGGGG 
CTCATGAAGA GCTACTTTGG GGGCCTGCTC TGTGTGTGCT GGAGCCCCGA CGGGCGCTAC 
GTCGTGACGG GGGGCGAAGA CGACCTGGTC ACCGTGTGGT CCTTCACCGA GGGCCGCGTG 
GTGGCCCGGG GCCACGGCCA CAAGTCCTGG GTCAACGCCG TGGCCTTCGA CCCCTACACC 
ACGAGGGCGG AGGAGGCAGC GGCCGCGGGC GCTGACGAGG AGCGGAGTGG CGAGGAGGAG 
GAGGAGCCGG AGGCGGGGAG CACAGGCTCG GGCGGGGGCG CCCCCCTCTC CCCGCTGCCC 
AAGGCCGGCT CCATCACGTA CCGCTTCGGC TCAGCCGGCC AGGACACGCA GTTCTGCCTG 
TGGGACCTCA CGGAAGACGT GCTCTACCCA CACCCTCCCC TGGCCCGCAC GCGCACCCTC 
CCAGGCACGC CTGGCACCAC GCCGCCTACC GCCAGCGGCT CGCGGGGCGG CGAGCCTGGC 
CCCGGGCCCC TGCCCCGTTC ACTGTCCCGC TCCAACAGCC TTCCGCACCC CGCGGGCAGC 
GGCAAGGCGG GTGGCCCGGG CACGGCAGCA GAGCCTGGCA CGCCCTTCAG CATCGGCCGC 
TTTGCCACGC TCACGCTGCA GGAGCGGCGG GACCGGGGGG CCGAGAAAGA GCACAAGCGC 
TACCACAGTC TGGGCAACAT CAGCCGGGGT GGCAGCGGGG GCGGGGGCGG CGGGGACAAG 
CCCAGCGGTG CCGCCCCCCG AAGCCGGCTG GACCCCGCCA AGGTGCTGGG CACCGCGCTT 
TGCCCGCGCA TCCACGAGGT ACCGCTGCTG GAGCCACTGG TATGCAAGAA GATCGCCCAG 
GAGCGGCTCA CGGTCCTCCT CTTCCTGGAG GACTGCATCA TCACCGCCTG CCAGGAGGGC 
CTCATCTGCA CCTGGGCCCG GCCGGGCAAG GCGTTCACAG ATGAGGAGAC CGAGGCCCAG 
ACAGGGGAAG GAAGTTGGCC CAGGTCACCC AGCAAGTCAG TGGTAGAGGG CATCTCCTCC 
CAGCCAGGCA ACTCCCCAAG CGGCACAGTG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002695143.1
CDS	197..2212
Translation	MAAGGAEGGSGPGAAMGDCAEIKSQFRTREGFYKLLPGDGAARRSGPASAQTPAPPQPPQPPPGPASASGPGAAGPAPSPPPAGPGPGPALPAVRLSLVRLGEPDSAGAGEPPATPAGLGAGGDRVCFNLGRELYFYPGCCRRGSQRSIDLNKPIDKRIYKGTQPTCHDFNQFTAATETISLLVGFSAGQVQYLDLIKKDTSKLFNEERLIDKTKVTYLKWLPESESLFLASHASGHLYLYNVGHPCASAPPQYSLLKQGEGFAVYAAKSKAPRNPLAKWAVGEGPLNEFAFSPDGRHLACVSQDGCLRVFHFDSMLLRGLMKSYFGGLLCVCWSPDGRYVVTGGEDDLVTVWSFTEGRVVARGHGHKSWVNAVAFDPYTTRAEEAAAAGADEERSGEEEEEPEAGSTGSGGGAPLSPLPKAGSITYRFGSAGQDTQFCLWDLTEDVLYPHPPLARTRTLPGTPGTTPPTASGSRGGEPGPGPLPRSLSRSNSLPHPAGSGKAGGPGTAAEPGTPFSIGRFATLTLQERRDRGAEKEHKRYHSLGNISRGGSGGGGGGDKPSGAAPRSRLDPAKVLGTALCPRIHEVPLLEPLVCKKIAQERLTVLLFLEDCIITACQEGLICTWARPGKAFTDEETEAQTGEGSWPRSPSKSVVEGISSQPGNSPSGTVV

Target ORF information:

RefSeq Version	XM_002695097.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dystrophia myotonica, WD repeat containing (DMWD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002695097.3

ORF Insert Sequence:

ATGGCGGCGG GCGGAGCGGA GGGCGGCTCG GGCCCCGGCG CCGCCATGGG GGATTGCGCG 
GAAATCAAGT CGCAGTTCCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGGC 
GCCGCCCGCA GATCGGGTCC GGCTTCCGCC CAGACCCCGG CGCCGCCCCA GCCGCCGCAG 
CCCCCGCCCG GCCCTGCCTC TGCCTCGGGC CCAGGCGCCG CGGGCCCCGC TCCGTCCCCT 
CCGCCTGCAG GCCCCGGGCC CGGGCCCGCG CTGCCTGCGG TGCGCCTAAG CCTCGTGCGT 
CTCGGGGAGC CCGACAGCGC CGGGGCCGGG GAGCCGCCTG CCACGCCCGC GGGACTGGGC 
GCCGGGGGAG ACCGCGTCTG CTTCAACTTG GGCCGCGAAC TCTATTTCTA CCCGGGCTGC 
TGTCGCCGTG GGAGCCAACG GTCCATTGAC CTCAACAAGC CAATTGACAA GCGGATCTAT 
AAGGGCACTC AGCCCACCTG CCATGACTTC AACCAGTTCA CTGCTGCCAC GGAGACCATC 
TCCCTGCTGG TGGGCTTCTC AGCCGGTCAA GTCCAGTACC TGGATCTCAT CAAGAAGGAC 
ACCAGCAAAC TATTCAATGA GGAGCGGCTC ATTGACAAGA CCAAGGTGAC ATATCTGAAG 
TGGCTGCCCG AGTCAGAGAG CCTGTTCCTG GCGTCCCACG CCAGCGGCCA CCTGTACCTG 
TACAACGTCG GCCACCCGTG CGCCTCGGCG CCGCCCCAGT ATAGCCTGCT GAAGCAGGGC 
GAGGGCTTCG CCGTCTACGC CGCCAAGAGC AAGGCGCCCC GCAACCCGCT GGCCAAGTGG 
GCGGTGGGCG AGGGGCCCCT CAACGAGTTC GCCTTCTCGC CCGACGGCCG GCACCTGGCC 
TGCGTCAGCC AGGATGGCTG CCTGCGCGTC TTCCACTTCG ATTCCATGCT CCTGCGGGGG 
CTCATGAAGA GCTACTTTGG GGGCCTGCTC TGTGTGTGCT GGAGCCCCGA CGGGCGCTAC 
GTCGTGACGG GGGGCGAAGA CGACCTGGTC ACCGTGTGGT CCTTCACCGA GGGCCGCGTG 
GTGGCCCGGG GCCACGGCCA CAAGTCCTGG GTCAACGCCG TGGCCTTCGA CCCCTACACC 
ACGAGGGCGG AGGAGGCAGC GGCCGCGGGC GCTGACGAGG AGCGGAGTGG CGAGGAGGAG 
GAGGAGCCGG AGGCGGGGAG CACAGGCTCG GGCGGGGGCG CCCCCCTCTC CCCGCTGCCC 
AAGGCCGGCT CCATCACGTA CCGCTTCGGC TCAGCCGGCC AGGACACGCA GTTCTGCCTG 
TGGGACCTCA CGGAAGACGT GCTCTACCCA CACCCTCCCC TGGCCCGCAC GCGCACCCTC 
CCAGGCACGC CTGGCACCAC GCCGCCTACC GCCAGCGGCT CGCGGGGCGG CGAGCCTGGC 
CCCGGGCCCC TGCCCCGTTC ACTGTCCCGC TCCAACAGCC TTCCGCACCC CGCGGGCAGC 
GGCAAGGCGG GTGGCCCGGG CACGGCAGCA GAGCCTGGCA CGCCCTTCAG CATCGGCCGC 
TTTGCCACGC TCACGCTGCA GGAGCGGCGG GACCGGGGGG CCGAGAAAGA GCACAAGCGC 
TACCACAGTC TGGGCAACAT CAGCCGGGGT GGCAGCGGGG GCGGGGGCGG CGGGGACAAG 
CCCAGCGGTG CCGCCCCCCG AAGCCGGCTG GACCCCGCCA AGGTGCTGGG CACCGCGCTT 
TGCCCGCGCA TCCACGAGGT ACCGCTGCTG GAGCCACTGG TATGCAAGAA GATCGCCCAG 
GAGCGGCTCA CGGTCCTCCT CTTCCTGGAG GACTGCATCA TCACCGCCTG CCAGGAGGGC 
CTCATCTGCA CCTGGGCCCG GCCGGGCAAG GCGTTCACAG ATGAGGAGAC CGAGGCCCAG 
ACAGGGGAAG GAAGTTGGCC CAGGTCACCC AGCAAGTCAG TGGTAGAGGG CATCTCCTCC 
CAGCCAGGCA ACTCCCCAAG CGGCACAGTG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMWD ( XM_010823770.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822072.2 Bos taurus dystrophia myotonica, WD repeat containing (DMWD), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa29011
Clone ID Related Accession (Same CDS sequence)	XM_002695097.3 , XM_010823770.2 , XM_002695097.4
Accession Version	XM_010823770.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dystrophia myotonica WD repeat-containing protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646390.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823770.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GCGGAGCGGA GGGCGGCTCG GGCCCCGGCG CCGCCATGGG GGATTGCGCG 
GAAATCAAGT CGCAGTTCCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGGC 
GCCGCCCGCA GATCGGGTCC GGCTTCCGCC CAGACCCCGG CGCCGCCCCA GCCGCCGCAG 
CCCCCGCCCG GCCCTGCCTC TGCCTCGGGC CCAGGCGCCG CGGGCCCCGC TCCGTCCCCT 
CCGCCTGCAG GCCCCGGGCC CGGGCCCGCG CTGCCTGCGG TGCGCCTAAG CCTCGTGCGT 
CTCGGGGAGC CCGACAGCGC CGGGGCCGGG GAGCCGCCTG CCACGCCCGC GGGACTGGGC 
GCCGGGGGAG ACCGCGTCTG CTTCAACTTG GGCCGCGAAC TCTATTTCTA CCCGGGCTGC 
TGTCGCCGTG GGAGCCAACG GTCCATTGAC CTCAACAAGC CAATTGACAA GCGGATCTAT 
AAGGGCACTC AGCCCACCTG CCATGACTTC AACCAGTTCA CTGCTGCCAC GGAGACCATC 
TCCCTGCTGG TGGGCTTCTC AGCCGGTCAA GTCCAGTACC TGGATCTCAT CAAGAAGGAC 
ACCAGCAAAC TATTCAATGA GGAGCGGCTC ATTGACAAGA CCAAGGTGAC ATATCTGAAG 
TGGCTGCCCG AGTCAGAGAG CCTGTTCCTG GCGTCCCACG CCAGCGGCCA CCTGTACCTG 
TACAACGTCG GCCACCCGTG CGCCTCGGCG CCGCCCCAGT ATAGCCTGCT GAAGCAGGGC 
GAGGGCTTCG CCGTCTACGC CGCCAAGAGC AAGGCGCCCC GCAACCCGCT GGCCAAGTGG 
GCGGTGGGCG AGGGGCCCCT CAACGAGTTC GCCTTCTCGC CCGACGGCCG GCACCTGGCC 
TGCGTCAGCC AGGATGGCTG CCTGCGCGTC TTCCACTTCG ATTCCATGCT CCTGCGGGGG 
CTCATGAAGA GCTACTTTGG GGGCCTGCTC TGTGTGTGCT GGAGCCCCGA CGGGCGCTAC 
GTCGTGACGG GGGGCGAAGA CGACCTGGTC ACCGTGTGGT CCTTCACCGA GGGCCGCGTG 
GTGGCCCGGG GCCACGGCCA CAAGTCCTGG GTCAACGCCG TGGCCTTCGA CCCCTACACC 
ACGAGGGCGG AGGAGGCAGC GGCCGCGGGC GCTGACGAGG AGCGGAGTGG CGAGGAGGAG 
GAGGAGCCGG AGGCGGGGAG CACAGGCTCG GGCGGGGGCG CCCCCCTCTC CCCGCTGCCC 
AAGGCCGGCT CCATCACGTA CCGCTTCGGC TCAGCCGGCC AGGACACGCA GTTCTGCCTG 
TGGGACCTCA CGGAAGACGT GCTCTACCCA CACCCTCCCC TGGCCCGCAC GCGCACCCTC 
CCAGGCACGC CTGGCACCAC GCCGCCTACC GCCAGCGGCT CGCGGGGCGG CGAGCCTGGC 
CCCGGGCCCC TGCCCCGTTC ACTGTCCCGC TCCAACAGCC TTCCGCACCC CGCGGGCAGC 
GGCAAGGCGG GTGGCCCGGG CACGGCAGCA GAGCCTGGCA CGCCCTTCAG CATCGGCCGC 
TTTGCCACGC TCACGCTGCA GGAGCGGCGG GACCGGGGGG CCGAGAAAGA GCACAAGCGC 
TACCACAGTC TGGGCAACAT CAGCCGGGGT GGCAGCGGGG GCGGGGGCGG CGGGGACAAG 
CCCAGCGGTG CCGCCCCCCG AAGCCGGCTG GACCCCGCCA AGGTGCTGGG CACCGCGCTT 
TGCCCGCGCA TCCACGAGGT ACCGCTGCTG GAGCCACTGG TATGCAAGAA GATCGCCCAG 
GAGCGGCTCA CGGTCCTCCT CTTCCTGGAG GACTGCATCA TCACCGCCTG CCAGGAGGGC 
CTCATCTGCA CCTGGGCCCG GCCGGGCAAG GCGTTCACAG ATGAGGAGAC CGAGGCCCAG 
ACAGGGGAAG GAAGTTGGCC CAGGTCACCC AGCAAGTCAG TGGTAGAGGG CATCTCCTCC 
CAGCCAGGCA ACTCCCCAAG CGGCACAGTG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822072.2
CDS	197..2212
Translation	MAAGGAEGGSGPGAAMGDCAEIKSQFRTREGFYKLLPGDGAARRSGPASAQTPAPPQPPQPPPGPASASGPGAAGPAPSPPPAGPGPGPALPAVRLSLVRLGEPDSAGAGEPPATPAGLGAGGDRVCFNLGRELYFYPGCCRRGSQRSIDLNKPIDKRIYKGTQPTCHDFNQFTAATETISLLVGFSAGQVQYLDLIKKDTSKLFNEERLIDKTKVTYLKWLPESESLFLASHASGHLYLYNVGHPCASAPPQYSLLKQGEGFAVYAAKSKAPRNPLAKWAVGEGPLNEFAFSPDGRHLACVSQDGCLRVFHFDSMLLRGLMKSYFGGLLCVCWSPDGRYVVTGGEDDLVTVWSFTEGRVVARGHGHKSWVNAVAFDPYTTRAEEAAAAGADEERSGEEEEEPEAGSTGSGGGAPLSPLPKAGSITYRFGSAGQDTQFCLWDLTEDVLYPHPPLARTRTLPGTPGTTPPTASGSRGGEPGPGPLPRSLSRSNSLPHPAGSGKAGGPGTAAEPGTPFSIGRFATLTLQERRDRGAEKEHKRYHSLGNISRGGSGGGGGGDKPSGAAPRSRLDPAKVLGTALCPRIHEVPLLEPLVCKKIAQERLTVLLFLEDCIITACQEGLICTWARPGKAFTDEETEAQTGEGSWPRSPSKSVVEGISSQPGNSPSGTVV

Target ORF information:

RefSeq Version	XM_010823770.2
Organism	Bos taurus(cattle)
Definition	Bos taurus dystrophia myotonica, WD repeat containing (DMWD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010823770.2

ORF Insert Sequence:

ATGGCGGCGG GCGGAGCGGA GGGCGGCTCG GGCCCCGGCG CCGCCATGGG GGATTGCGCG 
GAAATCAAGT CGCAGTTCCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGGC 
GCCGCCCGCA GATCGGGTCC GGCTTCCGCC CAGACCCCGG CGCCGCCCCA GCCGCCGCAG 
CCCCCGCCCG GCCCTGCCTC TGCCTCGGGC CCAGGCGCCG CGGGCCCCGC TCCGTCCCCT 
CCGCCTGCAG GCCCCGGGCC CGGGCCCGCG CTGCCTGCGG TGCGCCTAAG CCTCGTGCGT 
CTCGGGGAGC CCGACAGCGC CGGGGCCGGG GAGCCGCCTG CCACGCCCGC GGGACTGGGC 
GCCGGGGGAG ACCGCGTCTG CTTCAACTTG GGCCGCGAAC TCTATTTCTA CCCGGGCTGC 
TGTCGCCGTG GGAGCCAACG GTCCATTGAC CTCAACAAGC CAATTGACAA GCGGATCTAT 
AAGGGCACTC AGCCCACCTG CCATGACTTC AACCAGTTCA CTGCTGCCAC GGAGACCATC 
TCCCTGCTGG TGGGCTTCTC AGCCGGTCAA GTCCAGTACC TGGATCTCAT CAAGAAGGAC 
ACCAGCAAAC TATTCAATGA GGAGCGGCTC ATTGACAAGA CCAAGGTGAC ATATCTGAAG 
TGGCTGCCCG AGTCAGAGAG CCTGTTCCTG GCGTCCCACG CCAGCGGCCA CCTGTACCTG 
TACAACGTCG GCCACCCGTG CGCCTCGGCG CCGCCCCAGT ATAGCCTGCT GAAGCAGGGC 
GAGGGCTTCG CCGTCTACGC CGCCAAGAGC AAGGCGCCCC GCAACCCGCT GGCCAAGTGG 
GCGGTGGGCG AGGGGCCCCT CAACGAGTTC GCCTTCTCGC CCGACGGCCG GCACCTGGCC 
TGCGTCAGCC AGGATGGCTG CCTGCGCGTC TTCCACTTCG ATTCCATGCT CCTGCGGGGG 
CTCATGAAGA GCTACTTTGG GGGCCTGCTC TGTGTGTGCT GGAGCCCCGA CGGGCGCTAC 
GTCGTGACGG GGGGCGAAGA CGACCTGGTC ACCGTGTGGT CCTTCACCGA GGGCCGCGTG 
GTGGCCCGGG GCCACGGCCA CAAGTCCTGG GTCAACGCCG TGGCCTTCGA CCCCTACACC 
ACGAGGGCGG AGGAGGCAGC GGCCGCGGGC GCTGACGAGG AGCGGAGTGG CGAGGAGGAG 
GAGGAGCCGG AGGCGGGGAG CACAGGCTCG GGCGGGGGCG CCCCCCTCTC CCCGCTGCCC 
AAGGCCGGCT CCATCACGTA CCGCTTCGGC TCAGCCGGCC AGGACACGCA GTTCTGCCTG 
TGGGACCTCA CGGAAGACGT GCTCTACCCA CACCCTCCCC TGGCCCGCAC GCGCACCCTC 
CCAGGCACGC CTGGCACCAC GCCGCCTACC GCCAGCGGCT CGCGGGGCGG CGAGCCTGGC 
CCCGGGCCCC TGCCCCGTTC ACTGTCCCGC TCCAACAGCC TTCCGCACCC CGCGGGCAGC 
GGCAAGGCGG GTGGCCCGGG CACGGCAGCA GAGCCTGGCA CGCCCTTCAG CATCGGCCGC 
TTTGCCACGC TCACGCTGCA GGAGCGGCGG GACCGGGGGG CCGAGAAAGA GCACAAGCGC 
TACCACAGTC TGGGCAACAT CAGCCGGGGT GGCAGCGGGG GCGGGGGCGG CGGGGACAAG 
CCCAGCGGTG CCGCCCCCCG AAGCCGGCTG GACCCCGCCA AGGTGCTGGG CACCGCGCTT 
TGCCCGCGCA TCCACGAGGT ACCGCTGCTG GAGCCACTGG TATGCAAGAA GATCGCCCAG 
GAGCGGCTCA CGGTCCTCCT CTTCCTGGAG GACTGCATCA TCACCGCCTG CCAGGAGGGC 
CTCATCTGCA CCTGGGCCCG GCCGGGCAAG GCGTTCACAG ATGAGGAGAC CGAGGCCCAG 
ACAGGGGAAG GAAGTTGGCC CAGGTCACCC AGCAAGTCAG TGGTAGAGGG CATCTCCTCC 
CAGCCAGGCA ACTCCCCAAG CGGCACAGTG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMWD ( XM_002695097.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002695143.1 Bos taurus DM1 locus, WD repeat containing (DMWD), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa29011
Clone ID Related Accession (Same CDS sequence)	XM_002695097.3 , XM_010823770.2 , XM_002695097.4
Accession Version	XM_002695097.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystrophia myotonica WD repeat-containing protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002695097.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GCGGAGCGGA GGGCGGCTCG GGCCCCGGCG CCGCCATGGG GGATTGCGCG 
GAAATCAAGT CGCAGTTCCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGGC 
GCCGCCCGCA GATCGGGTCC GGCTTCCGCC CAGACCCCGG CGCCGCCCCA GCCGCCGCAG 
CCCCCGCCCG GCCCTGCCTC TGCCTCGGGC CCAGGCGCCG CGGGCCCCGC TCCGTCCCCT 
CCGCCTGCAG GCCCCGGGCC CGGGCCCGCG CTGCCTGCGG TGCGCCTAAG CCTCGTGCGT 
CTCGGGGAGC CCGACAGCGC CGGGGCCGGG GAGCCGCCTG CCACGCCCGC GGGACTGGGC 
GCCGGGGGAG ACCGCGTCTG CTTCAACTTG GGCCGCGAAC TCTATTTCTA CCCGGGCTGC 
TGTCGCCGTG GGAGCCAACG GTCCATTGAC CTCAACAAGC CAATTGACAA GCGGATCTAT 
AAGGGCACTC AGCCCACCTG CCATGACTTC AACCAGTTCA CTGCTGCCAC GGAGACCATC 
TCCCTGCTGG TGGGCTTCTC AGCCGGTCAA GTCCAGTACC TGGATCTCAT CAAGAAGGAC 
ACCAGCAAAC TATTCAATGA GGAGCGGCTC ATTGACAAGA CCAAGGTGAC ATATCTGAAG 
TGGCTGCCCG AGTCAGAGAG CCTGTTCCTG GCGTCCCACG CCAGCGGCCA CCTGTACCTG 
TACAACGTCG GCCACCCGTG CGCCTCGGCG CCGCCCCAGT ATAGCCTGCT GAAGCAGGGC 
GAGGGCTTCG CCGTCTACGC CGCCAAGAGC AAGGCGCCCC GCAACCCGCT GGCCAAGTGG 
GCGGTGGGCG AGGGGCCCCT CAACGAGTTC GCCTTCTCGC CCGACGGCCG GCACCTGGCC 
TGCGTCAGCC AGGATGGCTG CCTGCGCGTC TTCCACTTCG ATTCCATGCT CCTGCGGGGG 
CTCATGAAGA GCTACTTTGG GGGCCTGCTC TGTGTGTGCT GGAGCCCCGA CGGGCGCTAC 
GTCGTGACGG GGGGCGAAGA CGACCTGGTC ACCGTGTGGT CCTTCACCGA GGGCCGCGTG 
GTGGCCCGGG GCCACGGCCA CAAGTCCTGG GTCAACGCCG TGGCCTTCGA CCCCTACACC 
ACGAGGGCGG AGGAGGCAGC GGCCGCGGGC GCTGACGAGG AGCGGAGTGG CGAGGAGGAG 
GAGGAGCCGG AGGCGGGGAG CACAGGCTCG GGCGGGGGCG CCCCCCTCTC CCCGCTGCCC 
AAGGCCGGCT CCATCACGTA CCGCTTCGGC TCAGCCGGCC AGGACACGCA GTTCTGCCTG 
TGGGACCTCA CGGAAGACGT GCTCTACCCA CACCCTCCCC TGGCCCGCAC GCGCACCCTC 
CCAGGCACGC CTGGCACCAC GCCGCCTACC GCCAGCGGCT CGCGGGGCGG CGAGCCTGGC 
CCCGGGCCCC TGCCCCGTTC ACTGTCCCGC TCCAACAGCC TTCCGCACCC CGCGGGCAGC 
GGCAAGGCGG GTGGCCCGGG CACGGCAGCA GAGCCTGGCA CGCCCTTCAG CATCGGCCGC 
TTTGCCACGC TCACGCTGCA GGAGCGGCGG GACCGGGGGG CCGAGAAAGA GCACAAGCGC 
TACCACAGTC TGGGCAACAT CAGCCGGGGT GGCAGCGGGG GCGGGGGCGG CGGGGACAAG 
CCCAGCGGTG CCGCCCCCCG AAGCCGGCTG GACCCCGCCA AGGTGCTGGG CACCGCGCTT 
TGCCCGCGCA TCCACGAGGT ACCGCTGCTG GAGCCACTGG TATGCAAGAA GATCGCCCAG 
GAGCGGCTCA CGGTCCTCCT CTTCCTGGAG GACTGCATCA TCACCGCCTG CCAGGAGGGC 
CTCATCTGCA CCTGGGCCCG GCCGGGCAAG GCGTTCACAG ATGAGGAGAC CGAGGCCCAG 
ACAGGGGAAG GAAGTTGGCC CAGGTCACCC AGCAAGTCAG TGGTAGAGGG CATCTCCTCC 
CAGCCAGGCA ACTCCCCAAG CGGCACAGTG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002695143.1
CDS	197..2212
Translation	MAAGGAEGGSGPGAAMGDCAEIKSQFRTREGFYKLLPGDGAARRSGPASAQTPAPPQPPQPPPGPASASGPGAAGPAPSPPPAGPGPGPALPAVRLSLVRLGEPDSAGAGEPPATPAGLGAGGDRVCFNLGRELYFYPGCCRRGSQRSIDLNKPIDKRIYKGTQPTCHDFNQFTAATETISLLVGFSAGQVQYLDLIKKDTSKLFNEERLIDKTKVTYLKWLPESESLFLASHASGHLYLYNVGHPCASAPPQYSLLKQGEGFAVYAAKSKAPRNPLAKWAVGEGPLNEFAFSPDGRHLACVSQDGCLRVFHFDSMLLRGLMKSYFGGLLCVCWSPDGRYVVTGGEDDLVTVWSFTEGRVVARGHGHKSWVNAVAFDPYTTRAEEAAAAGADEERSGEEEEEPEAGSTGSGGGAPLSPLPKAGSITYRFGSAGQDTQFCLWDLTEDVLYPHPPLARTRTLPGTPGTTPPTASGSRGGEPGPGPLPRSLSRSNSLPHPAGSGKAGGPGTAAEPGTPFSIGRFATLTLQERRDRGAEKEHKRYHSLGNISRGGSGGGGGGDKPSGAAPRSRLDPAKVLGTALCPRIHEVPLLEPLVCKKIAQERLTVLLFLEDCIITACQEGLICTWARPGKAFTDEETEAQTGEGSWPRSPSKSVVEGISSQPGNSPSGTVV

Target ORF information:

RefSeq Version	XM_002695097.4
Organism	Bos taurus(cattle)
Definition	Bos taurus DM1 locus, WD repeat containing (DMWD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002695097.4

ORF Insert Sequence:

ATGGCGGCGG GCGGAGCGGA GGGCGGCTCG GGCCCCGGCG CCGCCATGGG GGATTGCGCG 
GAAATCAAGT CGCAGTTCCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGGC 
GCCGCCCGCA GATCGGGTCC GGCTTCCGCC CAGACCCCGG CGCCGCCCCA GCCGCCGCAG 
CCCCCGCCCG GCCCTGCCTC TGCCTCGGGC CCAGGCGCCG CGGGCCCCGC TCCGTCCCCT 
CCGCCTGCAG GCCCCGGGCC CGGGCCCGCG CTGCCTGCGG TGCGCCTAAG CCTCGTGCGT 
CTCGGGGAGC CCGACAGCGC CGGGGCCGGG GAGCCGCCTG CCACGCCCGC GGGACTGGGC 
GCCGGGGGAG ACCGCGTCTG CTTCAACTTG GGCCGCGAAC TCTATTTCTA CCCGGGCTGC 
TGTCGCCGTG GGAGCCAACG GTCCATTGAC CTCAACAAGC CAATTGACAA GCGGATCTAT 
AAGGGCACTC AGCCCACCTG CCATGACTTC AACCAGTTCA CTGCTGCCAC GGAGACCATC 
TCCCTGCTGG TGGGCTTCTC AGCCGGTCAA GTCCAGTACC TGGATCTCAT CAAGAAGGAC 
ACCAGCAAAC TATTCAATGA GGAGCGGCTC ATTGACAAGA CCAAGGTGAC ATATCTGAAG 
TGGCTGCCCG AGTCAGAGAG CCTGTTCCTG GCGTCCCACG CCAGCGGCCA CCTGTACCTG 
TACAACGTCG GCCACCCGTG CGCCTCGGCG CCGCCCCAGT ATAGCCTGCT GAAGCAGGGC 
GAGGGCTTCG CCGTCTACGC CGCCAAGAGC AAGGCGCCCC GCAACCCGCT GGCCAAGTGG 
GCGGTGGGCG AGGGGCCCCT CAACGAGTTC GCCTTCTCGC CCGACGGCCG GCACCTGGCC 
TGCGTCAGCC AGGATGGCTG CCTGCGCGTC TTCCACTTCG ATTCCATGCT CCTGCGGGGG 
CTCATGAAGA GCTACTTTGG GGGCCTGCTC TGTGTGTGCT GGAGCCCCGA CGGGCGCTAC 
GTCGTGACGG GGGGCGAAGA CGACCTGGTC ACCGTGTGGT CCTTCACCGA GGGCCGCGTG 
GTGGCCCGGG GCCACGGCCA CAAGTCCTGG GTCAACGCCG TGGCCTTCGA CCCCTACACC 
ACGAGGGCGG AGGAGGCAGC GGCCGCGGGC GCTGACGAGG AGCGGAGTGG CGAGGAGGAG 
GAGGAGCCGG AGGCGGGGAG CACAGGCTCG GGCGGGGGCG CCCCCCTCTC CCCGCTGCCC 
AAGGCCGGCT CCATCACGTA CCGCTTCGGC TCAGCCGGCC AGGACACGCA GTTCTGCCTG 
TGGGACCTCA CGGAAGACGT GCTCTACCCA CACCCTCCCC TGGCCCGCAC GCGCACCCTC 
CCAGGCACGC CTGGCACCAC GCCGCCTACC GCCAGCGGCT CGCGGGGCGG CGAGCCTGGC 
CCCGGGCCCC TGCCCCGTTC ACTGTCCCGC TCCAACAGCC TTCCGCACCC CGCGGGCAGC 
GGCAAGGCGG GTGGCCCGGG CACGGCAGCA GAGCCTGGCA CGCCCTTCAG CATCGGCCGC 
TTTGCCACGC TCACGCTGCA GGAGCGGCGG GACCGGGGGG CCGAGAAAGA GCACAAGCGC 
TACCACAGTC TGGGCAACAT CAGCCGGGGT GGCAGCGGGG GCGGGGGCGG CGGGGACAAG 
CCCAGCGGTG CCGCCCCCCG AAGCCGGCTG GACCCCGCCA AGGTGCTGGG CACCGCGCTT 
TGCCCGCGCA TCCACGAGGT ACCGCTGCTG GAGCCACTGG TATGCAAGAA GATCGCCCAG 
GAGCGGCTCA CGGTCCTCCT CTTCCTGGAG GACTGCATCA TCACCGCCTG CCAGGAGGGC 
CTCATCTGCA CCTGGGCCCG GCCGGGCAAG GCGTTCACAG ATGAGGAGAC CGAGGCCCAG 
ACAGGGGAAG GAAGTTGGCC CAGGTCACCC AGCAAGTCAG TGGTAGAGGG CATCTCCTCC 
CAGCCAGGCA ACTCCCCAAG CGGCACAGTG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMWD ( XM_005219442.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005219499.1 Bos taurus DM1 locus, WD repeat containing (DMWD), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa29012
Clone ID Related Accession (Same CDS sequence)	XM_005219442.3 , XM_005219442.2 , XM_015467596.1
Accession Version	XM_005219442.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystrophia myotonica WD repeat-containing protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005219442.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GCGGAGCGGA GGGCGGCTCG GGCCCCGGCG CCGCCATGGG GGATTGCGCG 
GAAATCAAGT CGCAGTTCCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGGC 
GCCGCCCGCA GATCGGGTCC GGCTTCCGCC CAGACCCCGG CGCCGCCCCA GCCGCCGCAG 
CCCCCGCCCG GCCCTGCCTC TGCCTCGGGC CCAGGCGCCG CGGGCCCCGC TCCGTCCCCT 
CCGCCTGCAG GCCCCGGGCC CGGGCCCGCG CTGCCTGCGG TGCGCCTAAG CCTCGTGCGT 
CTCGGGGAGC CCGACAGCGC CGGGGCCGGG GAGCCGCCTG CCACGCCCGC GGGACTGGGC 
GCCGGGGGAG ACCGCGTCTG CTTCAACTTG GGCCGCGAAC TCTATTTCTA CCCGGGCTGC 
TGTCGCCGTG GGAGCCAACG GTCCATTGAC CTCAACAAGC CAATTGACAA GCGGATCTAT 
AAGGGCACTC AGCCCACCTG CCATGACTTC AACCAGTTCA CTGCTGCCAC GGAGACCATC 
TCCCTGCTGG TGGGCTTCTC AGCCGGTCAA GTCCAGTACC TGGATCTCAT CAAGAAGGAC 
ACCAGCAAAC TATTCAATGA GGAGCGGCTC ATTGACAAGA CCAAGGTGAC ATATCTGAAG 
TGGCTGCCCG AGTCAGAGAG CCTGTTCCTG GCGTCCCACG CCAGCGGCCA CCTGTACCTG 
TACAACGTCG GCCACCCGTG CGCCTCGGCG CCGCCCCAGT ATAGCCTGCT GAAGCAGGGC 
GAGGGCTTCG CCGTCTACGC CGCCAAGAGC AAGGCGCCCC GCAACCCGCT GGCCAAGTGG 
GCGGTGGGCG AGGGGCCCCT CAACGAGTTC GCCTTCTCGC CCGACGGCCG GCACCTGGCC 
TGCGTCAGCC AGGATGGCTG CCTGCGCGTC TTCCACTTCG ATTCCATGCT CCTGCGGGGG 
CTCATGAAGA GCTACTTTGG GGGCCTGCTC TGTGTGTGCT GGAGCCCCGA CGGGCGCTAC 
GTCGTGACGG GGGGCGAAGA CGACCTGGTC ACCGTGTGGT CCTTCACCGA GGGCCGCGTG 
GTGGCCCGGG GCCACGGCCA CAAGTCCTGG GTCAACGCCG TGGCCTTCGA CCCCTACACC 
ACGAGGGCGG AGGAGGCAGC GGCCGCGGGC GCTGACGAGG AGCGGAGTGG CGAGGAGGAG 
GAGGAGCCGG AGGCGGGGAG CACAGGCTCG GGCGGGGGCG CCCCCCTCTC CCCGCTGCCC 
AAGGCCGGCT CCATCACGTA CCGCTTCGGC TCAGCCGGCC AGGACACGCA GTTCTGCCTG 
TGGGACCTCA CGGAAGACGT GCTCTACCCA CACCCTCCCC TGGCCCGCAC GCGCACCCTC 
CCAGGCACGC CTGGCACCAC GCCGCCTACC GCCAGCGGCT CGCGGGGCGG CGAGCCTGGC 
CCCGGGCCCC TGCCCCGTTC ACTGTCCCGC TCCAACAGCC TTCCGCACCC CGCGGGCAGC 
GGCAAGGCGG GTGGCCCGGG CACGGCAGCA GAGCCTGGCA CGCCCTTCAG CATCGGCCGC 
TTTGCCACGC TCACGCTGCA GGAGCGGCGG GACCGGGGGG CCGAGAAAGA GCACAAGCGC 
TACCACAGTC TGGGCAACAT CAGCCGGGGT GGCAGCGGGG GCGGGGGCGG CGGGGACAAG 
CCCAGCGGTG CCGCCCCCCG AAGCCGGCTG GACCCCGCCA AGGTGCTGGG CACCGCGCTT 
TGCCCGCGCA TCCACGAGGT ACCGCTGCTG GAGCCACTGG TATGCAAGAA GATCGCCCAG 
GAGCGGCTCA CGGTCCTCCT CTTCCTGGAG GACTGCATCA TCACCGCCTG CCAGGAGGGC 
CTCATCTGCA CCTGGGCCCG GCCGGGCAAG GCGGGCATCT CCTCCCAGCC AGGCAACTCC 
CCAAGCGGCA CAGTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005219499.1
CDS	197..2137
Translation	MAAGGAEGGSGPGAAMGDCAEIKSQFRTREGFYKLLPGDGAARRSGPASAQTPAPPQPPQPPPGPASASGPGAAGPAPSPPPAGPGPGPALPAVRLSLVRLGEPDSAGAGEPPATPAGLGAGGDRVCFNLGRELYFYPGCCRRGSQRSIDLNKPIDKRIYKGTQPTCHDFNQFTAATETISLLVGFSAGQVQYLDLIKKDTSKLFNEERLIDKTKVTYLKWLPESESLFLASHASGHLYLYNVGHPCASAPPQYSLLKQGEGFAVYAAKSKAPRNPLAKWAVGEGPLNEFAFSPDGRHLACVSQDGCLRVFHFDSMLLRGLMKSYFGGLLCVCWSPDGRYVVTGGEDDLVTVWSFTEGRVVARGHGHKSWVNAVAFDPYTTRAEEAAAAGADEERSGEEEEEPEAGSTGSGGGAPLSPLPKAGSITYRFGSAGQDTQFCLWDLTEDVLYPHPPLARTRTLPGTPGTTPPTASGSRGGEPGPGPLPRSLSRSNSLPHPAGSGKAGGPGTAAEPGTPFSIGRFATLTLQERRDRGAEKEHKRYHSLGNISRGGSGGGGGGDKPSGAAPRSRLDPAKVLGTALCPRIHEVPLLEPLVCKKIAQERLTVLLFLEDCIITACQEGLICTWARPGKAGISSQPGNSPSGTVV

Target ORF information:

RefSeq Version	XM_005219442.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DM1 locus, WD repeat containing (DMWD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005219442.3

ORF Insert Sequence:

ATGGCGGCGG GCGGAGCGGA GGGCGGCTCG GGCCCCGGCG CCGCCATGGG GGATTGCGCG 
GAAATCAAGT CGCAGTTCCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGGC 
GCCGCCCGCA GATCGGGTCC GGCTTCCGCC CAGACCCCGG CGCCGCCCCA GCCGCCGCAG 
CCCCCGCCCG GCCCTGCCTC TGCCTCGGGC CCAGGCGCCG CGGGCCCCGC TCCGTCCCCT 
CCGCCTGCAG GCCCCGGGCC CGGGCCCGCG CTGCCTGCGG TGCGCCTAAG CCTCGTGCGT 
CTCGGGGAGC CCGACAGCGC CGGGGCCGGG GAGCCGCCTG CCACGCCCGC GGGACTGGGC 
GCCGGGGGAG ACCGCGTCTG CTTCAACTTG GGCCGCGAAC TCTATTTCTA CCCGGGCTGC 
TGTCGCCGTG GGAGCCAACG GTCCATTGAC CTCAACAAGC CAATTGACAA GCGGATCTAT 
AAGGGCACTC AGCCCACCTG CCATGACTTC AACCAGTTCA CTGCTGCCAC GGAGACCATC 
TCCCTGCTGG TGGGCTTCTC AGCCGGTCAA GTCCAGTACC TGGATCTCAT CAAGAAGGAC 
ACCAGCAAAC TATTCAATGA GGAGCGGCTC ATTGACAAGA CCAAGGTGAC ATATCTGAAG 
TGGCTGCCCG AGTCAGAGAG CCTGTTCCTG GCGTCCCACG CCAGCGGCCA CCTGTACCTG 
TACAACGTCG GCCACCCGTG CGCCTCGGCG CCGCCCCAGT ATAGCCTGCT GAAGCAGGGC 
GAGGGCTTCG CCGTCTACGC CGCCAAGAGC AAGGCGCCCC GCAACCCGCT GGCCAAGTGG 
GCGGTGGGCG AGGGGCCCCT CAACGAGTTC GCCTTCTCGC CCGACGGCCG GCACCTGGCC 
TGCGTCAGCC AGGATGGCTG CCTGCGCGTC TTCCACTTCG ATTCCATGCT CCTGCGGGGG 
CTCATGAAGA GCTACTTTGG GGGCCTGCTC TGTGTGTGCT GGAGCCCCGA CGGGCGCTAC 
GTCGTGACGG GGGGCGAAGA CGACCTGGTC ACCGTGTGGT CCTTCACCGA GGGCCGCGTG 
GTGGCCCGGG GCCACGGCCA CAAGTCCTGG GTCAACGCCG TGGCCTTCGA CCCCTACACC 
ACGAGGGCGG AGGAGGCAGC GGCCGCGGGC GCTGACGAGG AGCGGAGTGG CGAGGAGGAG 
GAGGAGCCGG AGGCGGGGAG CACAGGCTCG GGCGGGGGCG CCCCCCTCTC CCCGCTGCCC 
AAGGCCGGCT CCATCACGTA CCGCTTCGGC TCAGCCGGCC AGGACACGCA GTTCTGCCTG 
TGGGACCTCA CGGAAGACGT GCTCTACCCA CACCCTCCCC TGGCCCGCAC GCGCACCCTC 
CCAGGCACGC CTGGCACCAC GCCGCCTACC GCCAGCGGCT CGCGGGGCGG CGAGCCTGGC 
CCCGGGCCCC TGCCCCGTTC ACTGTCCCGC TCCAACAGCC TTCCGCACCC CGCGGGCAGC 
GGCAAGGCGG GTGGCCCGGG CACGGCAGCA GAGCCTGGCA CGCCCTTCAG CATCGGCCGC 
TTTGCCACGC TCACGCTGCA GGAGCGGCGG GACCGGGGGG CCGAGAAAGA GCACAAGCGC 
TACCACAGTC TGGGCAACAT CAGCCGGGGT GGCAGCGGGG GCGGGGGCGG CGGGGACAAG 
CCCAGCGGTG CCGCCCCCCG AAGCCGGCTG GACCCCGCCA AGGTGCTGGG CACCGCGCTT 
TGCCCGCGCA TCCACGAGGT ACCGCTGCTG GAGCCACTGG TATGCAAGAA GATCGCCCAG 
GAGCGGCTCA CGGTCCTCCT CTTCCTGGAG GACTGCATCA TCACCGCCTG CCAGGAGGGC 
CTCATCTGCA CCTGGGCCCG GCCGGGCAAG GCGGGCATCT CCTCCCAGCC AGGCAACTCC 
CCAAGCGGCA CAGTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMWD ( XM_005219442.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005219499.1 Bos taurus dystrophia myotonica, WD repeat containing (DMWD), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa29012
Clone ID Related Accession (Same CDS sequence)	XM_005219442.3 , XM_005219442.2 , XM_015467596.1
Accession Version	XM_005219442.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dystrophia myotonica WD repeat-containing protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005219442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GCGGAGCGGA GGGCGGCTCG GGCCCCGGCG CCGCCATGGG GGATTGCGCG 
GAAATCAAGT CGCAGTTCCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGGC 
GCCGCCCGCA GATCGGGTCC GGCTTCCGCC CAGACCCCGG CGCCGCCCCA GCCGCCGCAG 
CCCCCGCCCG GCCCTGCCTC TGCCTCGGGC CCAGGCGCCG CGGGCCCCGC TCCGTCCCCT 
CCGCCTGCAG GCCCCGGGCC CGGGCCCGCG CTGCCTGCGG TGCGCCTAAG CCTCGTGCGT 
CTCGGGGAGC CCGACAGCGC CGGGGCCGGG GAGCCGCCTG CCACGCCCGC GGGACTGGGC 
GCCGGGGGAG ACCGCGTCTG CTTCAACTTG GGCCGCGAAC TCTATTTCTA CCCGGGCTGC 
TGTCGCCGTG GGAGCCAACG GTCCATTGAC CTCAACAAGC CAATTGACAA GCGGATCTAT 
AAGGGCACTC AGCCCACCTG CCATGACTTC AACCAGTTCA CTGCTGCCAC GGAGACCATC 
TCCCTGCTGG TGGGCTTCTC AGCCGGTCAA GTCCAGTACC TGGATCTCAT CAAGAAGGAC 
ACCAGCAAAC TATTCAATGA GGAGCGGCTC ATTGACAAGA CCAAGGTGAC ATATCTGAAG 
TGGCTGCCCG AGTCAGAGAG CCTGTTCCTG GCGTCCCACG CCAGCGGCCA CCTGTACCTG 
TACAACGTCG GCCACCCGTG CGCCTCGGCG CCGCCCCAGT ATAGCCTGCT GAAGCAGGGC 
GAGGGCTTCG CCGTCTACGC CGCCAAGAGC AAGGCGCCCC GCAACCCGCT GGCCAAGTGG 
GCGGTGGGCG AGGGGCCCCT CAACGAGTTC GCCTTCTCGC CCGACGGCCG GCACCTGGCC 
TGCGTCAGCC AGGATGGCTG CCTGCGCGTC TTCCACTTCG ATTCCATGCT CCTGCGGGGG 
CTCATGAAGA GCTACTTTGG GGGCCTGCTC TGTGTGTGCT GGAGCCCCGA CGGGCGCTAC 
GTCGTGACGG GGGGCGAAGA CGACCTGGTC ACCGTGTGGT CCTTCACCGA GGGCCGCGTG 
GTGGCCCGGG GCCACGGCCA CAAGTCCTGG GTCAACGCCG TGGCCTTCGA CCCCTACACC 
ACGAGGGCGG AGGAGGCAGC GGCCGCGGGC GCTGACGAGG AGCGGAGTGG CGAGGAGGAG 
GAGGAGCCGG AGGCGGGGAG CACAGGCTCG GGCGGGGGCG CCCCCCTCTC CCCGCTGCCC 
AAGGCCGGCT CCATCACGTA CCGCTTCGGC TCAGCCGGCC AGGACACGCA GTTCTGCCTG 
TGGGACCTCA CGGAAGACGT GCTCTACCCA CACCCTCCCC TGGCCCGCAC GCGCACCCTC 
CCAGGCACGC CTGGCACCAC GCCGCCTACC GCCAGCGGCT CGCGGGGCGG CGAGCCTGGC 
CCCGGGCCCC TGCCCCGTTC ACTGTCCCGC TCCAACAGCC TTCCGCACCC CGCGGGCAGC 
GGCAAGGCGG GTGGCCCGGG CACGGCAGCA GAGCCTGGCA CGCCCTTCAG CATCGGCCGC 
TTTGCCACGC TCACGCTGCA GGAGCGGCGG GACCGGGGGG CCGAGAAAGA GCACAAGCGC 
TACCACAGTC TGGGCAACAT CAGCCGGGGT GGCAGCGGGG GCGGGGGCGG CGGGGACAAG 
CCCAGCGGTG CCGCCCCCCG AAGCCGGCTG GACCCCGCCA AGGTGCTGGG CACCGCGCTT 
TGCCCGCGCA TCCACGAGGT ACCGCTGCTG GAGCCACTGG TATGCAAGAA GATCGCCCAG 
GAGCGGCTCA CGGTCCTCCT CTTCCTGGAG GACTGCATCA TCACCGCCTG CCAGGAGGGC 
CTCATCTGCA CCTGGGCCCG GCCGGGCAAG GCGGGCATCT CCTCCCAGCC AGGCAACTCC 
CCAAGCGGCA CAGTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005219499.1
CDS	197..2137
Translation	MAAGGAEGGSGPGAAMGDCAEIKSQFRTREGFYKLLPGDGAARRSGPASAQTPAPPQPPQPPPGPASASGPGAAGPAPSPPPAGPGPGPALPAVRLSLVRLGEPDSAGAGEPPATPAGLGAGGDRVCFNLGRELYFYPGCCRRGSQRSIDLNKPIDKRIYKGTQPTCHDFNQFTAATETISLLVGFSAGQVQYLDLIKKDTSKLFNEERLIDKTKVTYLKWLPESESLFLASHASGHLYLYNVGHPCASAPPQYSLLKQGEGFAVYAAKSKAPRNPLAKWAVGEGPLNEFAFSPDGRHLACVSQDGCLRVFHFDSMLLRGLMKSYFGGLLCVCWSPDGRYVVTGGEDDLVTVWSFTEGRVVARGHGHKSWVNAVAFDPYTTRAEEAAAAGADEERSGEEEEEPEAGSTGSGGGAPLSPLPKAGSITYRFGSAGQDTQFCLWDLTEDVLYPHPPLARTRTLPGTPGTTPPTASGSRGGEPGPGPLPRSLSRSNSLPHPAGSGKAGGPGTAAEPGTPFSIGRFATLTLQERRDRGAEKEHKRYHSLGNISRGGSGGGGGGDKPSGAAPRSRLDPAKVLGTALCPRIHEVPLLEPLVCKKIAQERLTVLLFLEDCIITACQEGLICTWARPGKAGISSQPGNSPSGTVV

Target ORF information:

RefSeq Version	XM_005219442.2
Organism	Bos taurus(cattle)
Definition	Bos taurus dystrophia myotonica, WD repeat containing (DMWD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005219442.2

ORF Insert Sequence:

ATGGCGGCGG GCGGAGCGGA GGGCGGCTCG GGCCCCGGCG CCGCCATGGG GGATTGCGCG 
GAAATCAAGT CGCAGTTCCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGGC 
GCCGCCCGCA GATCGGGTCC GGCTTCCGCC CAGACCCCGG CGCCGCCCCA GCCGCCGCAG 
CCCCCGCCCG GCCCTGCCTC TGCCTCGGGC CCAGGCGCCG CGGGCCCCGC TCCGTCCCCT 
CCGCCTGCAG GCCCCGGGCC CGGGCCCGCG CTGCCTGCGG TGCGCCTAAG CCTCGTGCGT 
CTCGGGGAGC CCGACAGCGC CGGGGCCGGG GAGCCGCCTG CCACGCCCGC GGGACTGGGC 
GCCGGGGGAG ACCGCGTCTG CTTCAACTTG GGCCGCGAAC TCTATTTCTA CCCGGGCTGC 
TGTCGCCGTG GGAGCCAACG GTCCATTGAC CTCAACAAGC CAATTGACAA GCGGATCTAT 
AAGGGCACTC AGCCCACCTG CCATGACTTC AACCAGTTCA CTGCTGCCAC GGAGACCATC 
TCCCTGCTGG TGGGCTTCTC AGCCGGTCAA GTCCAGTACC TGGATCTCAT CAAGAAGGAC 
ACCAGCAAAC TATTCAATGA GGAGCGGCTC ATTGACAAGA CCAAGGTGAC ATATCTGAAG 
TGGCTGCCCG AGTCAGAGAG CCTGTTCCTG GCGTCCCACG CCAGCGGCCA CCTGTACCTG 
TACAACGTCG GCCACCCGTG CGCCTCGGCG CCGCCCCAGT ATAGCCTGCT GAAGCAGGGC 
GAGGGCTTCG CCGTCTACGC CGCCAAGAGC AAGGCGCCCC GCAACCCGCT GGCCAAGTGG 
GCGGTGGGCG AGGGGCCCCT CAACGAGTTC GCCTTCTCGC CCGACGGCCG GCACCTGGCC 
TGCGTCAGCC AGGATGGCTG CCTGCGCGTC TTCCACTTCG ATTCCATGCT CCTGCGGGGG 
CTCATGAAGA GCTACTTTGG GGGCCTGCTC TGTGTGTGCT GGAGCCCCGA CGGGCGCTAC 
GTCGTGACGG GGGGCGAAGA CGACCTGGTC ACCGTGTGGT CCTTCACCGA GGGCCGCGTG 
GTGGCCCGGG GCCACGGCCA CAAGTCCTGG GTCAACGCCG TGGCCTTCGA CCCCTACACC 
ACGAGGGCGG AGGAGGCAGC GGCCGCGGGC GCTGACGAGG AGCGGAGTGG CGAGGAGGAG 
GAGGAGCCGG AGGCGGGGAG CACAGGCTCG GGCGGGGGCG CCCCCCTCTC CCCGCTGCCC 
AAGGCCGGCT CCATCACGTA CCGCTTCGGC TCAGCCGGCC AGGACACGCA GTTCTGCCTG 
TGGGACCTCA CGGAAGACGT GCTCTACCCA CACCCTCCCC TGGCCCGCAC GCGCACCCTC 
CCAGGCACGC CTGGCACCAC GCCGCCTACC GCCAGCGGCT CGCGGGGCGG CGAGCCTGGC 
CCCGGGCCCC TGCCCCGTTC ACTGTCCCGC TCCAACAGCC TTCCGCACCC CGCGGGCAGC 
GGCAAGGCGG GTGGCCCGGG CACGGCAGCA GAGCCTGGCA CGCCCTTCAG CATCGGCCGC 
TTTGCCACGC TCACGCTGCA GGAGCGGCGG GACCGGGGGG CCGAGAAAGA GCACAAGCGC 
TACCACAGTC TGGGCAACAT CAGCCGGGGT GGCAGCGGGG GCGGGGGCGG CGGGGACAAG 
CCCAGCGGTG CCGCCCCCCG AAGCCGGCTG GACCCCGCCA AGGTGCTGGG CACCGCGCTT 
TGCCCGCGCA TCCACGAGGT ACCGCTGCTG GAGCCACTGG TATGCAAGAA GATCGCCCAG 
GAGCGGCTCA CGGTCCTCCT CTTCCTGGAG GACTGCATCA TCACCGCCTG CCAGGAGGGC 
CTCATCTGCA CCTGGGCCCG GCCGGGCAAG GCGGGCATCT CCTCCCAGCC AGGCAACTCC 
CCAAGCGGCA CAGTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMWD ( XM_015467596.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323082.1 Bos taurus dystrophia myotonica, WD repeat containing (DMWD), transcript variant X2, mRNA.

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CloneID	OBa29012
Clone ID Related Accession (Same CDS sequence)	XM_005219442.3 , XM_005219442.2 , XM_015467596.1
Accession Version	XM_015467596.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dystrophia myotonica WD repeat-containing protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646390.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG GCGGAGCGGA GGGCGGCTCG GGCCCCGGCG CCGCCATGGG GGATTGCGCG 
GAAATCAAGT CGCAGTTCCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGGC 
GCCGCCCGCA GATCGGGTCC GGCTTCCGCC CAGACCCCGG CGCCGCCCCA GCCGCCGCAG 
CCCCCGCCCG GCCCTGCCTC TGCCTCGGGC CCAGGCGCCG CGGGCCCCGC TCCGTCCCCT 
CCGCCTGCAG GCCCCGGGCC CGGGCCCGCG CTGCCTGCGG TGCGCCTAAG CCTCGTGCGT 
CTCGGGGAGC CCGACAGCGC CGGGGCCGGG GAGCCGCCTG CCACGCCCGC GGGACTGGGC 
GCCGGGGGAG ACCGCGTCTG CTTCAACTTG GGCCGCGAAC TCTATTTCTA CCCGGGCTGC 
TGTCGCCGTG GGAGCCAACG GTCCATTGAC CTCAACAAGC CAATTGACAA GCGGATCTAT 
AAGGGCACTC AGCCCACCTG CCATGACTTC AACCAGTTCA CTGCTGCCAC GGAGACCATC 
TCCCTGCTGG TGGGCTTCTC AGCCGGTCAA GTCCAGTACC TGGATCTCAT CAAGAAGGAC 
ACCAGCAAAC TATTCAATGA GGAGCGGCTC ATTGACAAGA CCAAGGTGAC ATATCTGAAG 
TGGCTGCCCG AGTCAGAGAG CCTGTTCCTG GCGTCCCACG CCAGCGGCCA CCTGTACCTG 
TACAACGTCG GCCACCCGTG CGCCTCGGCG CCGCCCCAGT ATAGCCTGCT GAAGCAGGGC 
GAGGGCTTCG CCGTCTACGC CGCCAAGAGC AAGGCGCCCC GCAACCCGCT GGCCAAGTGG 
GCGGTGGGCG AGGGGCCCCT CAACGAGTTC GCCTTCTCGC CCGACGGCCG GCACCTGGCC 
TGCGTCAGCC AGGATGGCTG CCTGCGCGTC TTCCACTTCG ATTCCATGCT CCTGCGGGGG 
CTCATGAAGA GCTACTTTGG GGGCCTGCTC TGTGTGTGCT GGAGCCCCGA CGGGCGCTAC 
GTCGTGACGG GGGGCGAAGA CGACCTGGTC ACCGTGTGGT CCTTCACCGA GGGCCGCGTG 
GTGGCCCGGG GCCACGGCCA CAAGTCCTGG GTCAACGCCG TGGCCTTCGA CCCCTACACC 
ACGAGGGCGG AGGAGGCAGC GGCCGCGGGC GCTGACGAGG AGCGGAGTGG CGAGGAGGAG 
GAGGAGCCGG AGGCGGGGAG CACAGGCTCG GGCGGGGGCG CCCCCCTCTC CCCGCTGCCC 
AAGGCCGGCT CCATCACGTA CCGCTTCGGC TCAGCCGGCC AGGACACGCA GTTCTGCCTG 
TGGGACCTCA CGGAAGACGT GCTCTACCCA CACCCTCCCC TGGCCCGCAC GCGCACCCTC 
CCAGGCACGC CTGGCACCAC GCCGCCTACC GCCAGCGGCT CGCGGGGCGG CGAGCCTGGC 
CCCGGGCCCC TGCCCCGTTC ACTGTCCCGC TCCAACAGCC TTCCGCACCC CGCGGGCAGC 
GGCAAGGCGG GTGGCCCGGG CACGGCAGCA GAGCCTGGCA CGCCCTTCAG CATCGGCCGC 
TTTGCCACGC TCACGCTGCA GGAGCGGCGG GACCGGGGGG CCGAGAAAGA GCACAAGCGC 
TACCACAGTC TGGGCAACAT CAGCCGGGGT GGCAGCGGGG GCGGGGGCGG CGGGGACAAG 
CCCAGCGGTG CCGCCCCCCG AAGCCGGCTG GACCCCGCCA AGGTGCTGGG CACCGCGCTT 
TGCCCGCGCA TCCACGAGGT ACCGCTGCTG GAGCCACTGG TATGCAAGAA GATCGCCCAG 
GAGCGGCTCA CGGTCCTCCT CTTCCTGGAG GACTGCATCA TCACCGCCTG CCAGGAGGGC 
CTCATCTGCA CCTGGGCCCG GCCGGGCAAG GCGGGCATCT CCTCCCAGCC AGGCAACTCC 
CCAAGCGGCA CAGTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323082.1
CDS	197..2137
Translation	MAAGGAEGGSGPGAAMGDCAEIKSQFRTREGFYKLLPGDGAARRSGPASAQTPAPPQPPQPPPGPASASGPGAAGPAPSPPPAGPGPGPALPAVRLSLVRLGEPDSAGAGEPPATPAGLGAGGDRVCFNLGRELYFYPGCCRRGSQRSIDLNKPIDKRIYKGTQPTCHDFNQFTAATETISLLVGFSAGQVQYLDLIKKDTSKLFNEERLIDKTKVTYLKWLPESESLFLASHASGHLYLYNVGHPCASAPPQYSLLKQGEGFAVYAAKSKAPRNPLAKWAVGEGPLNEFAFSPDGRHLACVSQDGCLRVFHFDSMLLRGLMKSYFGGLLCVCWSPDGRYVVTGGEDDLVTVWSFTEGRVVARGHGHKSWVNAVAFDPYTTRAEEAAAAGADEERSGEEEEEPEAGSTGSGGGAPLSPLPKAGSITYRFGSAGQDTQFCLWDLTEDVLYPHPPLARTRTLPGTPGTTPPTASGSRGGEPGPGPLPRSLSRSNSLPHPAGSGKAGGPGTAAEPGTPFSIGRFATLTLQERRDRGAEKEHKRYHSLGNISRGGSGGGGGGDKPSGAAPRSRLDPAKVLGTALCPRIHEVPLLEPLVCKKIAQERLTVLLFLEDCIITACQEGLICTWARPGKAGISSQPGNSPSGTVV

Target ORF information:

RefSeq Version	XM_015467596.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dystrophia myotonica, WD repeat containing (DMWD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015467596.1

ORF Insert Sequence:

ATGGCGGCGG GCGGAGCGGA GGGCGGCTCG GGCCCCGGCG CCGCCATGGG GGATTGCGCG 
GAAATCAAGT CGCAGTTCCG CACCCGCGAG GGCTTCTACA AGCTGCTCCC CGGCGACGGC 
GCCGCCCGCA GATCGGGTCC GGCTTCCGCC CAGACCCCGG CGCCGCCCCA GCCGCCGCAG 
CCCCCGCCCG GCCCTGCCTC TGCCTCGGGC CCAGGCGCCG CGGGCCCCGC TCCGTCCCCT 
CCGCCTGCAG GCCCCGGGCC CGGGCCCGCG CTGCCTGCGG TGCGCCTAAG CCTCGTGCGT 
CTCGGGGAGC CCGACAGCGC CGGGGCCGGG GAGCCGCCTG CCACGCCCGC GGGACTGGGC 
GCCGGGGGAG ACCGCGTCTG CTTCAACTTG GGCCGCGAAC TCTATTTCTA CCCGGGCTGC 
TGTCGCCGTG GGAGCCAACG GTCCATTGAC CTCAACAAGC CAATTGACAA GCGGATCTAT 
AAGGGCACTC AGCCCACCTG CCATGACTTC AACCAGTTCA CTGCTGCCAC GGAGACCATC 
TCCCTGCTGG TGGGCTTCTC AGCCGGTCAA GTCCAGTACC TGGATCTCAT CAAGAAGGAC 
ACCAGCAAAC TATTCAATGA GGAGCGGCTC ATTGACAAGA CCAAGGTGAC ATATCTGAAG 
TGGCTGCCCG AGTCAGAGAG CCTGTTCCTG GCGTCCCACG CCAGCGGCCA CCTGTACCTG 
TACAACGTCG GCCACCCGTG CGCCTCGGCG CCGCCCCAGT ATAGCCTGCT GAAGCAGGGC 
GAGGGCTTCG CCGTCTACGC CGCCAAGAGC AAGGCGCCCC GCAACCCGCT GGCCAAGTGG 
GCGGTGGGCG AGGGGCCCCT CAACGAGTTC GCCTTCTCGC CCGACGGCCG GCACCTGGCC 
TGCGTCAGCC AGGATGGCTG CCTGCGCGTC TTCCACTTCG ATTCCATGCT CCTGCGGGGG 
CTCATGAAGA GCTACTTTGG GGGCCTGCTC TGTGTGTGCT GGAGCCCCGA CGGGCGCTAC 
GTCGTGACGG GGGGCGAAGA CGACCTGGTC ACCGTGTGGT CCTTCACCGA GGGCCGCGTG 
GTGGCCCGGG GCCACGGCCA CAAGTCCTGG GTCAACGCCG TGGCCTTCGA CCCCTACACC 
ACGAGGGCGG AGGAGGCAGC GGCCGCGGGC GCTGACGAGG AGCGGAGTGG CGAGGAGGAG 
GAGGAGCCGG AGGCGGGGAG CACAGGCTCG GGCGGGGGCG CCCCCCTCTC CCCGCTGCCC 
AAGGCCGGCT CCATCACGTA CCGCTTCGGC TCAGCCGGCC AGGACACGCA GTTCTGCCTG 
TGGGACCTCA CGGAAGACGT GCTCTACCCA CACCCTCCCC TGGCCCGCAC GCGCACCCTC 
CCAGGCACGC CTGGCACCAC GCCGCCTACC GCCAGCGGCT CGCGGGGCGG CGAGCCTGGC 
CCCGGGCCCC TGCCCCGTTC ACTGTCCCGC TCCAACAGCC TTCCGCACCC CGCGGGCAGC 
GGCAAGGCGG GTGGCCCGGG CACGGCAGCA GAGCCTGGCA CGCCCTTCAG CATCGGCCGC 
TTTGCCACGC TCACGCTGCA GGAGCGGCGG GACCGGGGGG CCGAGAAAGA GCACAAGCGC 
TACCACAGTC TGGGCAACAT CAGCCGGGGT GGCAGCGGGG GCGGGGGCGG CGGGGACAAG 
CCCAGCGGTG CCGCCCCCCG AAGCCGGCTG GACCCCGCCA AGGTGCTGGG CACCGCGCTT 
TGCCCGCGCA TCCACGAGGT ACCGCTGCTG GAGCCACTGG TATGCAAGAA GATCGCCCAG 
GAGCGGCTCA CGGTCCTCCT CTTCCTGGAG GACTGCATCA TCACCGCCTG CCAGGAGGGC 
CTCATCTGCA CCTGGGCCCG GCCGGGCAAG GCGGGCATCT CCTCCCAGCC AGGCAACTCC 
CCAAGCGGCA CAGTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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DMWD cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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