ANKEF1 cDNA ORF clone, Bos taurus(cattle)

The following ANKEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa07409 XM_002692038.5
Latest version!
Bos taurus ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa07409 XM_001255789.4
Latest version!
Bos taurus ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa221813 XM_025000908.1
Latest version!
Bos taurus ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa92215 XM_015473953.2
Latest version!
Bos taurus ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa92215 XM_015465856.1
Latest version!
Bos taurus ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OBa07409 XM_002692038.4 Bos taurus ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa92215 XM_015473953.1 Bos taurus ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa07409
    Clone ID Related Accession (Same CDS sequence) XM_002692038.4 , XM_001255789.4 , XM_002692038.5
    Accession Version XM_002692038.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2331bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ankyrin repeat and EF-hand domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104360.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002692038.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTTTAG CAGATAAGAG ACTCGAGAAC CTGCAGATCT ACAAAGTTCT TCAGTGTGTT 
    CGGAACAAAG ACAAGAAGCA GATAGAGAAG TTGACCAGGC TCGGATATCC TGAACTCATC
    AATTTCACAG ACCCAGTCAA TGGAAACAGT GCTCTGCACT TAGCCTCAGT TTCCAATGAC
    ATCGACATGG TCAGCTTTCT CCTGAGCCTT GGTGCTCACC CTGATGTGCA AGACAAAATG
    GGCTGTACTC CGACTATGAG GGCTGCAGAA CTGGGCCACG AATTGTCCAT GGAAATATTA
    GCCAAAGCAA AAGCCGATAT GACTGTTGTT GATAATGAAG GAAAAGGTAT TTTGTTTTAC
    TGCATTTTAC CTACTAAACG GCATTATCGG TGTGCACTGA TTGCCTTGGA ACATGGTGCG
    GATGTCAACA ATTGTACCTG TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGAAGCA
    CATGATGTTA AGGATGTGTG CCTGACGTTT TTGGAAAAAG GAGCCAATCC AAATGCTATC
    AACACATCCA CAGGTCGCAC GGCTTTAATG GAAGCATCAA GAGAGGGAGT GACAGAACTA
    GTTCGAGGGA TATTGGAAAG AGGAGGGGAC GTGAATGCAT TTGACAATGA CAGACATCAT
    GCTGCACATT TTGCCGCCAA AGGAGGCTTT TTTGATATAT TGAAGCTTCT TTTTGCATAC
    AACGGAGACA TGGGGTTGAT TGCAATGAAT GGCAACACAC CACTTCATTA TGCTGCCATG
    GGTGGTTTCG CAGATTGCTG TAAATATATA GCTCAGCGAG GATGTGACCT AAAATGGAAG
    AATCTGGAGC ATAAAACGCC CAGGAACTTG GCTAAGGAAG GCGGCTTCAA GGCAGCCAGC
    AAAGAAATAC GGCGGGCGGA GCAAATCGCT AATAAACTGG CCAGGCCAGG GGTCAAAAAT
    CCGAATCCGC ACTTGTTCCT GAGACTTCAC GATTGGTCAG TAGAACATGA GACTTTCCTT
    CGGGAAGCCT TCTCGTTTGT GGACAGGGGC GATGGGACAG TCACCAAGGA GGACTTTGTG
    CTGACCCTGG AGGAGAGGCA AGATTTTGTG AACTCAGAGC AGCTGGCTGC TATAGCGCAA
    CTTCACGAAA AAGTCCGGGG TGGAGGGGTC AATATTAACG AGTTCTTTAA AGGGACCAGA
    TATTTAAGTA AGTCTTATGT CTTGGGATCC TATGGGCCTA AGAAAAAGAA AAAAGGGATG
    GCCAAAAGAG CCAGAGTGGG CAAGTTTGTT TTCCCCCTCC CCATCTGTAT CATCCCCGAC
    CACGTGTTTC CACGCCGCAG TGATGGTGGG CCGCCCTATT ACATGATCGA AACCTACAAG
    AATGTAACTG ATTGCAACCG CTTTAACAGA GATCACCCCC CAGAACATCC CATTCAGGAT
    GACTCTGCTT GGTATATTGA TGATCCAGGG AAGGTCTTTT CAAACATTAA TTTTATCACC
    AAGGCGGGAG ACCTGGCTTC TCTGAAAAAG GCTTTTGAAT CAGGAATACC TGTGGATATG
    AAGGATAATT ACTACAAGAC ACCGCTAATG ACTGCATGTG CCACTGGAAA CATTGATGCA
    GTCAAGTTTC TTCTTGAAAA AGGGGCTAAT GTTAATGCAA CAGATAATTT TCTGTGGACT
    CCACTCCATT TTGCATGCCA TGCTGGCCAA CAGGATATTG TTGAGCTTCT TGTTAAATCT
    GGAGCCGTGG TAGATGCCCT CTCAATCAAC AACTCAACTC CCTTAAGTAG AGCCATTGAG
    AGCTGCAGAC TGGATACTGT AAAATACCTA CTTGACATCG GTGCTAAATT CCAGTTGGAG
    AATAGGAAAG GGCATAGTGC CATGGATGTT GCAAAAGCTT ATGCTGACAG TAGAATAATT
    GGTTTGATTA AAGAAAAGCT GGATAACTTG CCCAAACCAG CAGAAAATCA AAAACCAAAA
    GGCAAGCCAC AACCCAAAGC GAAGACTGAA GGCCCCGAGA TTAAGAAGGA AGAGGAAACA
    CTTTCAGCAA TATACACTGT ACCAGCCATA GTGGAGGACA AGAAGATGCG TAAAGATAAT
    GTAGTTTATC TTAATTCATT GATTACCAGT GGTTATACCA AGAAAGTGGA TATCACATTT
    ATCCCACAGA GGATCTGGAG TCCTGAAGCC ACGACAGCAG AGCTGATCAG AATGAGGGAA
    CTGCGCCGGG AGAGGTTGAC ATATGAGGTA GATTTTGATG ACTTCATGAT GCCCTTTCAG
    AAGCACATCA CAGAGAGAGC ACAAGCAGTG GAAGCTAGCT TCAAGATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002692084.1
    CDS359..2689
    Translation

    Target ORF information:

    RefSeq Version XM_002692038.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002692038.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTTTAG CAGATAAGAG ACTCGAGAAC CTGCAGATCT ACAAAGTTCT TCAGTGTGTT 
    CGGAACAAAG ACAAGAAGCA GATAGAGAAG TTGACCAGGC TCGGATATCC TGAACTCATC
    AATTTCACAG ACCCAGTCAA TGGAAACAGT GCTCTGCACT TAGCCTCAGT TTCCAATGAC
    ATCGACATGG TCAGCTTTCT CCTGAGCCTT GGTGCTCACC CTGATGTGCA AGACAAAATG
    GGCTGTACTC CGACTATGAG GGCTGCAGAA CTGGGCCACG AATTGTCCAT GGAAATATTA
    GCCAAAGCAA AAGCCGATAT GACTGTTGTT GATAATGAAG GAAAAGGTAT TTTGTTTTAC
    TGCATTTTAC CTACTAAACG GCATTATCGG TGTGCACTGA TTGCCTTGGA ACATGGTGCG
    GATGTCAACA ATTGTACCTG TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGAAGCA
    CATGATGTTA AGGATGTGTG CCTGACGTTT TTGGAAAAAG GAGCCAATCC AAATGCTATC
    AACACATCCA CAGGTCGCAC GGCTTTAATG GAAGCATCAA GAGAGGGAGT GACAGAACTA
    GTTCGAGGGA TATTGGAAAG AGGAGGGGAC GTGAATGCAT TTGACAATGA CAGACATCAT
    GCTGCACATT TTGCCGCCAA AGGAGGCTTT TTTGATATAT TGAAGCTTCT TTTTGCATAC
    AACGGAGACA TGGGGTTGAT TGCAATGAAT GGCAACACAC CACTTCATTA TGCTGCCATG
    GGTGGTTTCG CAGATTGCTG TAAATATATA GCTCAGCGAG GATGTGACCT AAAATGGAAG
    AATCTGGAGC ATAAAACGCC CAGGAACTTG GCTAAGGAAG GCGGCTTCAA GGCAGCCAGC
    AAAGAAATAC GGCGGGCGGA GCAAATCGCT AATAAACTGG CCAGGCCAGG GGTCAAAAAT
    CCGAATCCGC ACTTGTTCCT GAGACTTCAC GATTGGTCAG TAGAACATGA GACTTTCCTT
    CGGGAAGCCT TCTCGTTTGT GGACAGGGGC GATGGGACAG TCACCAAGGA GGACTTTGTG
    CTGACCCTGG AGGAGAGGCA AGATTTTGTG AACTCAGAGC AGCTGGCTGC TATAGCGCAA
    CTTCACGAAA AAGTCCGGGG TGGAGGGGTC AATATTAACG AGTTCTTTAA AGGGACCAGA
    TATTTAAGTA AGTCTTATGT CTTGGGATCC TATGGGCCTA AGAAAAAGAA AAAAGGGATG
    GCCAAAAGAG CCAGAGTGGG CAAGTTTGTT TTCCCCCTCC CCATCTGTAT CATCCCCGAC
    CACGTGTTTC CACGCCGCAG TGATGGTGGG CCGCCCTATT ACATGATCGA AACCTACAAG
    AATGTAACTG ATTGCAACCG CTTTAACAGA GATCACCCCC CAGAACATCC CATTCAGGAT
    GACTCTGCTT GGTATATTGA TGATCCAGGG AAGGTCTTTT CAAACATTAA TTTTATCACC
    AAGGCGGGAG ACCTGGCTTC TCTGAAAAAG GCTTTTGAAT CAGGAATACC TGTGGATATG
    AAGGATAATT ACTACAAGAC ACCGCTAATG ACTGCATGTG CCACTGGAAA CATTGATGCA
    GTCAAGTTTC TTCTTGAAAA AGGGGCTAAT GTTAATGCAA CAGATAATTT TCTGTGGACT
    CCACTCCATT TTGCATGCCA TGCTGGCCAA CAGGATATTG TTGAGCTTCT TGTTAAATCT
    GGAGCCGTGG TAGATGCCCT CTCAATCAAC AACTCAACTC CCTTAAGTAG AGCCATTGAG
    AGCTGCAGAC TGGATACTGT AAAATACCTA CTTGACATCG GTGCTAAATT CCAGTTGGAG
    AATAGGAAAG GGCATAGTGC CATGGATGTT GCAAAAGCTT ATGCTGACAG TAGAATAATT
    GGTTTGATTA AAGAAAAGCT GGATAACTTG CCCAAACCAG CAGAAAATCA AAAACCAAAA
    GGCAAGCCAC AACCCAAAGC GAAGACTGAA GGCCCCGAGA TTAAGAAGGA AGAGGAAACA
    CTTTCAGCAA TATACACTGT ACCAGCCATA GTGGAGGACA AGAAGATGCG TAAAGATAAT
    GTAGTTTATC TTAATTCATT GATTACCAGT GGTTATACCA AGAAAGTGGA TATCACATTT
    ATCCCACAGA GGATCTGGAG TCCTGAAGCC ACGACAGCAG AGCTGATCAG AATGAGGGAA
    CTGCGCCGGG AGAGGTTGAC ATATGAGGTA GATTTTGATG ACTTCATGAT GCCCTTTCAG
    AAGCACATCA CAGAGAGAGC ACAAGCAGTG GAAGCTAGCT TCAAGATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07409
    Clone ID Related Accession (Same CDS sequence) XM_002692038.4 , XM_001255789.4 , XM_002692038.5
    Accession Version XM_001255789.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2331bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ankyrin repeat and EF-hand domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646117.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001255789.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTTTAG CAGATAAGAG ACTCGAGAAC CTGCAGATCT ACAAAGTTCT TCAGTGTGTT 
    CGGAACAAAG ACAAGAAGCA GATAGAGAAG TTGACCAGGC TCGGATATCC TGAACTCATC
    AATTTCACAG ACCCAGTCAA TGGAAACAGT GCTCTGCACT TAGCCTCAGT TTCCAATGAC
    ATCGACATGG TCAGCTTTCT CCTGAGCCTT GGTGCTCACC CTGATGTGCA AGACAAAATG
    GGCTGTACTC CGACTATGAG GGCTGCAGAA CTGGGCCACG AATTGTCCAT GGAAATATTA
    GCCAAAGCAA AAGCCGATAT GACTGTTGTT GATAATGAAG GAAAAGGTAT TTTGTTTTAC
    TGCATTTTAC CTACTAAACG GCATTATCGG TGTGCACTGA TTGCCTTGGA ACATGGTGCG
    GATGTCAACA ATTGTACCTG TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGAAGCA
    CATGATGTTA AGGATGTGTG CCTGACGTTT TTGGAAAAAG GAGCCAATCC AAATGCTATC
    AACACATCCA CAGGTCGCAC GGCTTTAATG GAAGCATCAA GAGAGGGAGT GACAGAACTA
    GTTCGAGGGA TATTGGAAAG AGGAGGGGAC GTGAATGCAT TTGACAATGA CAGACATCAT
    GCTGCACATT TTGCCGCCAA AGGAGGCTTT TTTGATATAT TGAAGCTTCT TTTTGCATAC
    AACGGAGACA TGGGGTTGAT TGCAATGAAT GGCAACACAC CACTTCATTA TGCTGCCATG
    GGTGGTTTCG CAGATTGCTG TAAATATATA GCTCAGCGAG GATGTGACCT AAAATGGAAG
    AATCTGGAGC ATAAAACGCC CAGGAACTTG GCTAAGGAAG GCGGCTTCAA GGCAGCCAGC
    AAAGAAATAC GGCGGGCGGA GCAAATCGCT AATAAACTGG CCAGGCCAGG GGTCAAAAAT
    CCGAATCCGC ACTTGTTCCT GAGACTTCAC GATTGGTCAG TAGAACATGA GACTTTCCTT
    CGGGAAGCCT TCTCGTTTGT GGACAGGGGC GATGGGACAG TCACCAAGGA GGACTTTGTG
    CTGACCCTGG AGGAGAGGCA AGATTTTGTG AACTCAGAGC AGCTGGCTGC TATAGCGCAA
    CTTCACGAAA AAGTCCGGGG TGGAGGGGTC AATATTAACG AGTTCTTTAA AGGGACCAGA
    TATTTAAGTA AGTCTTATGT CTTGGGATCC TATGGGCCTA AGAAAAAGAA AAAAGGGATG
    GCCAAAAGAG CCAGAGTGGG CAAGTTTGTT TTCCCCCTCC CCATCTGTAT CATCCCCGAC
    CACGTGTTTC CACGCCGCAG TGATGGTGGG CCGCCCTATT ACATGATCGA AACCTACAAG
    AATGTAACTG ATTGCAACCG CTTTAACAGA GATCACCCCC CAGAACATCC CATTCAGGAT
    GACTCTGCTT GGTATATTGA TGATCCAGGG AAGGTCTTTT CAAACATTAA TTTTATCACC
    AAGGCGGGAG ACCTGGCTTC TCTGAAAAAG GCTTTTGAAT CAGGAATACC TGTGGATATG
    AAGGATAATT ACTACAAGAC ACCGCTAATG ACTGCATGTG CCACTGGAAA CATTGATGCA
    GTCAAGTTTC TTCTTGAAAA AGGGGCTAAT GTTAATGCAA CAGATAATTT TCTGTGGACT
    CCACTCCATT TTGCATGCCA TGCTGGCCAA CAGGATATTG TTGAGCTTCT TGTTAAATCT
    GGAGCCGTGG TAGATGCCCT CTCAATCAAC AACTCAACTC CCTTAAGTAG AGCCATTGAG
    AGCTGCAGAC TGGATACTGT AAAATACCTA CTTGACATCG GTGCTAAATT CCAGTTGGAG
    AATAGGAAAG GGCATAGTGC CATGGATGTT GCAAAAGCTT ATGCTGACAG TAGAATAATT
    GGTTTGATTA AAGAAAAGCT GGATAACTTG CCCAAACCAG CAGAAAATCA AAAACCAAAA
    GGCAAGCCAC AACCCAAAGC GAAGACTGAA GGCCCCGAGA TTAAGAAGGA AGAGGAAACA
    CTTTCAGCAA TATACACTGT ACCAGCCATA GTGGAGGACA AGAAGATGCG TAAAGATAAT
    GTAGTTTATC TTAATTCATT GATTACCAGT GGTTATACCA AGAAAGTGGA TATCACATTT
    ATCCCACAGA GGATCTGGAG TCCTGAAGCC ACGACAGCAG AGCTGATCAG AATGAGGGAA
    CTGCGCCGGG AGAGGTTGAC ATATGAGGTA GATTTTGATG ACTTCATGAT GCCCTTTCAG
    AAGCACATCA CAGAGAGAGC ACAAGCAGTG GAAGCTAGCT TCAAGATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001255790.1
    CDS359..2689
    Translation

    Target ORF information:

    RefSeq Version XM_001255789.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001255789.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTTTAG CAGATAAGAG ACTCGAGAAC CTGCAGATCT ACAAAGTTCT TCAGTGTGTT 
    CGGAACAAAG ACAAGAAGCA GATAGAGAAG TTGACCAGGC TCGGATATCC TGAACTCATC
    AATTTCACAG ACCCAGTCAA TGGAAACAGT GCTCTGCACT TAGCCTCAGT TTCCAATGAC
    ATCGACATGG TCAGCTTTCT CCTGAGCCTT GGTGCTCACC CTGATGTGCA AGACAAAATG
    GGCTGTACTC CGACTATGAG GGCTGCAGAA CTGGGCCACG AATTGTCCAT GGAAATATTA
    GCCAAAGCAA AAGCCGATAT GACTGTTGTT GATAATGAAG GAAAAGGTAT TTTGTTTTAC
    TGCATTTTAC CTACTAAACG GCATTATCGG TGTGCACTGA TTGCCTTGGA ACATGGTGCG
    GATGTCAACA ATTGTACCTG TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGAAGCA
    CATGATGTTA AGGATGTGTG CCTGACGTTT TTGGAAAAAG GAGCCAATCC AAATGCTATC
    AACACATCCA CAGGTCGCAC GGCTTTAATG GAAGCATCAA GAGAGGGAGT GACAGAACTA
    GTTCGAGGGA TATTGGAAAG AGGAGGGGAC GTGAATGCAT TTGACAATGA CAGACATCAT
    GCTGCACATT TTGCCGCCAA AGGAGGCTTT TTTGATATAT TGAAGCTTCT TTTTGCATAC
    AACGGAGACA TGGGGTTGAT TGCAATGAAT GGCAACACAC CACTTCATTA TGCTGCCATG
    GGTGGTTTCG CAGATTGCTG TAAATATATA GCTCAGCGAG GATGTGACCT AAAATGGAAG
    AATCTGGAGC ATAAAACGCC CAGGAACTTG GCTAAGGAAG GCGGCTTCAA GGCAGCCAGC
    AAAGAAATAC GGCGGGCGGA GCAAATCGCT AATAAACTGG CCAGGCCAGG GGTCAAAAAT
    CCGAATCCGC ACTTGTTCCT GAGACTTCAC GATTGGTCAG TAGAACATGA GACTTTCCTT
    CGGGAAGCCT TCTCGTTTGT GGACAGGGGC GATGGGACAG TCACCAAGGA GGACTTTGTG
    CTGACCCTGG AGGAGAGGCA AGATTTTGTG AACTCAGAGC AGCTGGCTGC TATAGCGCAA
    CTTCACGAAA AAGTCCGGGG TGGAGGGGTC AATATTAACG AGTTCTTTAA AGGGACCAGA
    TATTTAAGTA AGTCTTATGT CTTGGGATCC TATGGGCCTA AGAAAAAGAA AAAAGGGATG
    GCCAAAAGAG CCAGAGTGGG CAAGTTTGTT TTCCCCCTCC CCATCTGTAT CATCCCCGAC
    CACGTGTTTC CACGCCGCAG TGATGGTGGG CCGCCCTATT ACATGATCGA AACCTACAAG
    AATGTAACTG ATTGCAACCG CTTTAACAGA GATCACCCCC CAGAACATCC CATTCAGGAT
    GACTCTGCTT GGTATATTGA TGATCCAGGG AAGGTCTTTT CAAACATTAA TTTTATCACC
    AAGGCGGGAG ACCTGGCTTC TCTGAAAAAG GCTTTTGAAT CAGGAATACC TGTGGATATG
    AAGGATAATT ACTACAAGAC ACCGCTAATG ACTGCATGTG CCACTGGAAA CATTGATGCA
    GTCAAGTTTC TTCTTGAAAA AGGGGCTAAT GTTAATGCAA CAGATAATTT TCTGTGGACT
    CCACTCCATT TTGCATGCCA TGCTGGCCAA CAGGATATTG TTGAGCTTCT TGTTAAATCT
    GGAGCCGTGG TAGATGCCCT CTCAATCAAC AACTCAACTC CCTTAAGTAG AGCCATTGAG
    AGCTGCAGAC TGGATACTGT AAAATACCTA CTTGACATCG GTGCTAAATT CCAGTTGGAG
    AATAGGAAAG GGCATAGTGC CATGGATGTT GCAAAAGCTT ATGCTGACAG TAGAATAATT
    GGTTTGATTA AAGAAAAGCT GGATAACTTG CCCAAACCAG CAGAAAATCA AAAACCAAAA
    GGCAAGCCAC AACCCAAAGC GAAGACTGAA GGCCCCGAGA TTAAGAAGGA AGAGGAAACA
    CTTTCAGCAA TATACACTGT ACCAGCCATA GTGGAGGACA AGAAGATGCG TAAAGATAAT
    GTAGTTTATC TTAATTCATT GATTACCAGT GGTTATACCA AGAAAGTGGA TATCACATTT
    ATCCCACAGA GGATCTGGAG TCCTGAAGCC ACGACAGCAG AGCTGATCAG AATGAGGGAA
    CTGCGCCGGG AGAGGTTGAC ATATGAGGTA GATTTTGATG ACTTCATGAT GCCCTTTCAG
    AAGCACATCA CAGAGAGAGC ACAAGCAGTG GAAGCTAGCT TCAAGATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07409
    Clone ID Related Accession (Same CDS sequence) XM_002692038.4 , XM_001255789.4 , XM_002692038.5
    Accession Version XM_002692038.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2331bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ankyrin repeat and EF-hand domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002692038.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTTTAG CAGATAAGAG ACTCGAGAAC CTGCAGATCT ACAAAGTTCT TCAGTGTGTT 
    CGGAACAAAG ACAAGAAGCA GATAGAGAAG TTGACCAGGC TCGGATATCC TGAACTCATC
    AATTTCACAG ACCCAGTCAA TGGAAACAGT GCTCTGCACT TAGCCTCAGT TTCCAATGAC
    ATCGACATGG TCAGCTTTCT CCTGAGCCTT GGTGCTCACC CTGATGTGCA AGACAAAATG
    GGCTGTACTC CGACTATGAG GGCTGCAGAA CTGGGCCACG AATTGTCCAT GGAAATATTA
    GCCAAAGCAA AAGCCGATAT GACTGTTGTT GATAATGAAG GAAAAGGTAT TTTGTTTTAC
    TGCATTTTAC CTACTAAACG GCATTATCGG TGTGCACTGA TTGCCTTGGA ACATGGTGCG
    GATGTCAACA ATTGTACCTG TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGAAGCA
    CATGATGTTA AGGATGTGTG CCTGACGTTT TTGGAAAAAG GAGCCAATCC AAATGCTATC
    AACACATCCA CAGGTCGCAC GGCTTTAATG GAAGCATCAA GAGAGGGAGT GACAGAACTA
    GTTCGAGGGA TATTGGAAAG AGGAGGGGAC GTGAATGCAT TTGACAATGA CAGACATCAT
    GCTGCACATT TTGCCGCCAA AGGAGGCTTT TTTGATATAT TGAAGCTTCT TTTTGCATAC
    AACGGAGACA TGGGGTTGAT TGCAATGAAT GGCAACACAC CACTTCATTA TGCTGCCATG
    GGTGGTTTCG CAGATTGCTG TAAATATATA GCTCAGCGAG GATGTGACCT AAAATGGAAG
    AATCTGGAGC ATAAAACGCC CAGGAACTTG GCTAAGGAAG GCGGCTTCAA GGCAGCCAGC
    AAAGAAATAC GGCGGGCGGA GCAAATCGCT AATAAACTGG CCAGGCCAGG GGTCAAAAAT
    CCGAATCCGC ACTTGTTCCT GAGACTTCAC GATTGGTCAG TAGAACATGA GACTTTCCTT
    CGGGAAGCCT TCTCGTTTGT GGACAGGGGC GATGGGACAG TCACCAAGGA GGACTTTGTG
    CTGACCCTGG AGGAGAGGCA AGATTTTGTG AACTCAGAGC AGCTGGCTGC TATAGCGCAA
    CTTCACGAAA AAGTCCGGGG TGGAGGGGTC AATATTAACG AGTTCTTTAA AGGGACCAGA
    TATTTAAGTA AGTCTTATGT CTTGGGATCC TATGGGCCTA AGAAAAAGAA AAAAGGGATG
    GCCAAAAGAG CCAGAGTGGG CAAGTTTGTT TTCCCCCTCC CCATCTGTAT CATCCCCGAC
    CACGTGTTTC CACGCCGCAG TGATGGTGGG CCGCCCTATT ACATGATCGA AACCTACAAG
    AATGTAACTG ATTGCAACCG CTTTAACAGA GATCACCCCC CAGAACATCC CATTCAGGAT
    GACTCTGCTT GGTATATTGA TGATCCAGGG AAGGTCTTTT CAAACATTAA TTTTATCACC
    AAGGCGGGAG ACCTGGCTTC TCTGAAAAAG GCTTTTGAAT CAGGAATACC TGTGGATATG
    AAGGATAATT ACTACAAGAC ACCGCTAATG ACTGCATGTG CCACTGGAAA CATTGATGCA
    GTCAAGTTTC TTCTTGAAAA AGGGGCTAAT GTTAATGCAA CAGATAATTT TCTGTGGACT
    CCACTCCATT TTGCATGCCA TGCTGGCCAA CAGGATATTG TTGAGCTTCT TGTTAAATCT
    GGAGCCGTGG TAGATGCCCT CTCAATCAAC AACTCAACTC CCTTAAGTAG AGCCATTGAG
    AGCTGCAGAC TGGATACTGT AAAATACCTA CTTGACATCG GTGCTAAATT CCAGTTGGAG
    AATAGGAAAG GGCATAGTGC CATGGATGTT GCAAAAGCTT ATGCTGACAG TAGAATAATT
    GGTTTGATTA AAGAAAAGCT GGATAACTTG CCCAAACCAG CAGAAAATCA AAAACCAAAA
    GGCAAGCCAC AACCCAAAGC GAAGACTGAA GGCCCCGAGA TTAAGAAGGA AGAGGAAACA
    CTTTCAGCAA TATACACTGT ACCAGCCATA GTGGAGGACA AGAAGATGCG TAAAGATAAT
    GTAGTTTATC TTAATTCATT GATTACCAGT GGTTATACCA AGAAAGTGGA TATCACATTT
    ATCCCACAGA GGATCTGGAG TCCTGAAGCC ACGACAGCAG AGCTGATCAG AATGAGGGAA
    CTGCGCCGGG AGAGGTTGAC ATATGAGGTA GATTTTGATG ACTTCATGAT GCCCTTTCAG
    AAGCACATCA CAGAGAGAGC ACAAGCAGTG GAAGCTAGCT TCAAGATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002692084.1
    CDS292..2622
    Translation

    Target ORF information:

    RefSeq Version XM_002692038.5
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002692038.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCTTTAG CAGATAAGAG ACTCGAGAAC CTGCAGATCT ACAAAGTTCT TCAGTGTGTT 
    CGGAACAAAG ACAAGAAGCA GATAGAGAAG TTGACCAGGC TCGGATATCC TGAACTCATC
    AATTTCACAG ACCCAGTCAA TGGAAACAGT GCTCTGCACT TAGCCTCAGT TTCCAATGAC
    ATCGACATGG TCAGCTTTCT CCTGAGCCTT GGTGCTCACC CTGATGTGCA AGACAAAATG
    GGCTGTACTC CGACTATGAG GGCTGCAGAA CTGGGCCACG AATTGTCCAT GGAAATATTA
    GCCAAAGCAA AAGCCGATAT GACTGTTGTT GATAATGAAG GAAAAGGTAT TTTGTTTTAC
    TGCATTTTAC CTACTAAACG GCATTATCGG TGTGCACTGA TTGCCTTGGA ACATGGTGCG
    GATGTCAACA ATTGTACCTG TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGAAGCA
    CATGATGTTA AGGATGTGTG CCTGACGTTT TTGGAAAAAG GAGCCAATCC AAATGCTATC
    AACACATCCA CAGGTCGCAC GGCTTTAATG GAAGCATCAA GAGAGGGAGT GACAGAACTA
    GTTCGAGGGA TATTGGAAAG AGGAGGGGAC GTGAATGCAT TTGACAATGA CAGACATCAT
    GCTGCACATT TTGCCGCCAA AGGAGGCTTT TTTGATATAT TGAAGCTTCT TTTTGCATAC
    AACGGAGACA TGGGGTTGAT TGCAATGAAT GGCAACACAC CACTTCATTA TGCTGCCATG
    GGTGGTTTCG CAGATTGCTG TAAATATATA GCTCAGCGAG GATGTGACCT AAAATGGAAG
    AATCTGGAGC ATAAAACGCC CAGGAACTTG GCTAAGGAAG GCGGCTTCAA GGCAGCCAGC
    AAAGAAATAC GGCGGGCGGA GCAAATCGCT AATAAACTGG CCAGGCCAGG GGTCAAAAAT
    CCGAATCCGC ACTTGTTCCT GAGACTTCAC GATTGGTCAG TAGAACATGA GACTTTCCTT
    CGGGAAGCCT TCTCGTTTGT GGACAGGGGC GATGGGACAG TCACCAAGGA GGACTTTGTG
    CTGACCCTGG AGGAGAGGCA AGATTTTGTG AACTCAGAGC AGCTGGCTGC TATAGCGCAA
    CTTCACGAAA AAGTCCGGGG TGGAGGGGTC AATATTAACG AGTTCTTTAA AGGGACCAGA
    TATTTAAGTA AGTCTTATGT CTTGGGATCC TATGGGCCTA AGAAAAAGAA AAAAGGGATG
    GCCAAAAGAG CCAGAGTGGG CAAGTTTGTT TTCCCCCTCC CCATCTGTAT CATCCCCGAC
    CACGTGTTTC CACGCCGCAG TGATGGTGGG CCGCCCTATT ACATGATCGA AACCTACAAG
    AATGTAACTG ATTGCAACCG CTTTAACAGA GATCACCCCC CAGAACATCC CATTCAGGAT
    GACTCTGCTT GGTATATTGA TGATCCAGGG AAGGTCTTTT CAAACATTAA TTTTATCACC
    AAGGCGGGAG ACCTGGCTTC TCTGAAAAAG GCTTTTGAAT CAGGAATACC TGTGGATATG
    AAGGATAATT ACTACAAGAC ACCGCTAATG ACTGCATGTG CCACTGGAAA CATTGATGCA
    GTCAAGTTTC TTCTTGAAAA AGGGGCTAAT GTTAATGCAA CAGATAATTT TCTGTGGACT
    CCACTCCATT TTGCATGCCA TGCTGGCCAA CAGGATATTG TTGAGCTTCT TGTTAAATCT
    GGAGCCGTGG TAGATGCCCT CTCAATCAAC AACTCAACTC CCTTAAGTAG AGCCATTGAG
    AGCTGCAGAC TGGATACTGT AAAATACCTA CTTGACATCG GTGCTAAATT CCAGTTGGAG
    AATAGGAAAG GGCATAGTGC CATGGATGTT GCAAAAGCTT ATGCTGACAG TAGAATAATT
    GGTTTGATTA AAGAAAAGCT GGATAACTTG CCCAAACCAG CAGAAAATCA AAAACCAAAA
    GGCAAGCCAC AACCCAAAGC GAAGACTGAA GGCCCCGAGA TTAAGAAGGA AGAGGAAACA
    CTTTCAGCAA TATACACTGT ACCAGCCATA GTGGAGGACA AGAAGATGCG TAAAGATAAT
    GTAGTTTATC TTAATTCATT GATTACCAGT GGTTATACCA AGAAAGTGGA TATCACATTT
    ATCCCACAGA GGATCTGGAG TCCTGAAGCC ACGACAGCAG AGCTGATCAG AATGAGGGAA
    CTGCGCCGGG AGAGGTTGAC ATATGAGGTA GATTTTGATG ACTTCATGAT GCCCTTTCAG
    AAGCACATCA CAGAGAGAGC ACAAGCAGTG GAAGCTAGCT TCAAGATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221813
    Clone ID Related Accession (Same CDS sequence) XM_025000908.1
    Accession Version XM_025000908.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ankyrin repeat and EF-hand domain-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAAGCAT CAAGAGAGGG AGTGACAGAA CTAGTTCGAG GGATATTGGA AAGAGGAGGG 
    GACGTGAATG CATTTGACAA TGACAGACAT CATGCTGCAC ATTTTGCCGC CAAAGGAGGC
    TTTTTTGATA TATTGAAGCT TCTTTTTGCA TACAACGGAG ACATGGGGTT GATTGCAATG
    AATGGCAACA CACCACTTCA TTATGCTGCC ATGGGTGGTT TCGCAGATTG CTGTAAATAT
    ATAGCTCAGC GAGGATGTGA CCTAAAATGG AAGAATCTGG AGCATAAAAC GCCCAGGAAC
    TTGGCTAAGG AAGGCGGCTT CAAGGCAGCC AGCAAAGAAA TACGGCGGGC GGAGCAAATC
    GCTAATAAAC TGGCCAGGCC AGGGGTCAAA AATCCGAATC CGCACTTGTT CCTGAGACTT
    CACGATTGGT CAGTAGAACA TGAGACTTTC CTTCGGGAAG CCTTCTCGTT TGTGGACAGG
    GGCGATGGGA CAGTCACCAA GGAGGACTTT GTGCTGACCC TGGAGGAGAG GCAAGATTTT
    GTGAACTCAG AGCAGCTGGC TGCTATAGCG CAACTTCACG AAAAAGTCCG GGGTGGAGGG
    GTCAATATTA ACGAGTTCTT TAAAGGGACC AGATATTTAA GTAAGTCTTA TGTCTTGGGA
    TCCTATGGGC CTAAGAAAAA GAAAAAAGGG ATGGCCAAAA GAGCCAGAGT GGGCAAGTTT
    GTTTTCCCCC TCCCCATCTG TATCATCCCC GACCACGTGT TTCCACGCCG CAGTGATGGT
    GGGCCGCCCT ATTACATGAT CGAAACCTAC AAGAATGTAA CTGATTGCAA CCGCTTTAAC
    AGAGATCACC CCCCAGAACA TCCCATTCAG GATGACTCTG CTTGGTATAT TGATGATCCA
    GGGAAGGTCT TTTCAAACAT TAATTTTATC ACCAAGGCGG GAGACCTGGC TTCTCTGAAA
    AAGGCTTTTG AATCAGGAAT ACCTGTGGAT ATGAAGGATA ATTACTACAA GACACCGCTA
    ATGACTGCAT GTGCCACTGG AAACATTGAT GCAGTCAAGT TTCTTCTTGA AAAAGGGGCT
    AATGTTAATG CAACAGATAA TTTTCTGTGG ACTCCACTCC ATTTTGCATG CCATGCTGGC
    CAACAGGATA TTGTTGAGCT TCTTGTTAAA TCTGGAGCCG TGGTAGATGC CCTCTCAATC
    AACAACTCAA CTCCCTTAAG TAGAGCCATT GAGAGCTGCA GACTGGATAC TGTAAAATAC
    CTACTTGACA TCGGTGCTAA ATTCCAGTTG GAGAATAGGA AAGGGCATAG TGCCATGGAT
    GTTGCAAAAG CTTATGCTGA CAGTAGAATA ATTGGTTTGA TTAAAGAAAA GCTGGATAAC
    TTGCCCAAAC CAGCAGAAAA TCAAAAACCA AAAGGCAAGC CACAACCCAA AGCGAAGACT
    GAAGGCCCCG AGATTAAGAA GGAAGAGGAA ACACTTTCAG CAATATACAC TGTACCAGCC
    ATAGTGGAGG ACAAGAAGAT GCGTAAAGAT AATGTAGTTT ATCTTAATTC ATTGATTACC
    AGTGGTTATA CCAAGAAAGT GGATATCACA TTTATCCCAC AGAGGATCTG GAGTCCTGAA
    GCCACGACAG CAGAGCTGAT CAGAATGAGG GAACTGCGCC GGGAGAGGTT GACATATGAG
    GTAGATTTTG ATGACTTCAT GATGCCCTTT CAGAAGCACA TCACAGAGAG AGCACAAGCA
    GTGGAAGCTA GCTTCAAGAT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856676.1
    CDS464..2227
    Translation

    Target ORF information:

    RefSeq Version XM_025000908.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000908.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAAGCAT CAAGAGAGGG AGTGACAGAA CTAGTTCGAG GGATATTGGA AAGAGGAGGG 
    GACGTGAATG CATTTGACAA TGACAGACAT CATGCTGCAC ATTTTGCCGC CAAAGGAGGC
    TTTTTTGATA TATTGAAGCT TCTTTTTGCA TACAACGGAG ACATGGGGTT GATTGCAATG
    AATGGCAACA CACCACTTCA TTATGCTGCC ATGGGTGGTT TCGCAGATTG CTGTAAATAT
    ATAGCTCAGC GAGGATGTGA CCTAAAATGG AAGAATCTGG AGCATAAAAC GCCCAGGAAC
    TTGGCTAAGG AAGGCGGCTT CAAGGCAGCC AGCAAAGAAA TACGGCGGGC GGAGCAAATC
    GCTAATAAAC TGGCCAGGCC AGGGGTCAAA AATCCGAATC CGCACTTGTT CCTGAGACTT
    CACGATTGGT CAGTAGAACA TGAGACTTTC CTTCGGGAAG CCTTCTCGTT TGTGGACAGG
    GGCGATGGGA CAGTCACCAA GGAGGACTTT GTGCTGACCC TGGAGGAGAG GCAAGATTTT
    GTGAACTCAG AGCAGCTGGC TGCTATAGCG CAACTTCACG AAAAAGTCCG GGGTGGAGGG
    GTCAATATTA ACGAGTTCTT TAAAGGGACC AGATATTTAA GTAAGTCTTA TGTCTTGGGA
    TCCTATGGGC CTAAGAAAAA GAAAAAAGGG ATGGCCAAAA GAGCCAGAGT GGGCAAGTTT
    GTTTTCCCCC TCCCCATCTG TATCATCCCC GACCACGTGT TTCCACGCCG CAGTGATGGT
    GGGCCGCCCT ATTACATGAT CGAAACCTAC AAGAATGTAA CTGATTGCAA CCGCTTTAAC
    AGAGATCACC CCCCAGAACA TCCCATTCAG GATGACTCTG CTTGGTATAT TGATGATCCA
    GGGAAGGTCT TTTCAAACAT TAATTTTATC ACCAAGGCGG GAGACCTGGC TTCTCTGAAA
    AAGGCTTTTG AATCAGGAAT ACCTGTGGAT ATGAAGGATA ATTACTACAA GACACCGCTA
    ATGACTGCAT GTGCCACTGG AAACATTGAT GCAGTCAAGT TTCTTCTTGA AAAAGGGGCT
    AATGTTAATG CAACAGATAA TTTTCTGTGG ACTCCACTCC ATTTTGCATG CCATGCTGGC
    CAACAGGATA TTGTTGAGCT TCTTGTTAAA TCTGGAGCCG TGGTAGATGC CCTCTCAATC
    AACAACTCAA CTCCCTTAAG TAGAGCCATT GAGAGCTGCA GACTGGATAC TGTAAAATAC
    CTACTTGACA TCGGTGCTAA ATTCCAGTTG GAGAATAGGA AAGGGCATAG TGCCATGGAT
    GTTGCAAAAG CTTATGCTGA CAGTAGAATA ATTGGTTTGA TTAAAGAAAA GCTGGATAAC
    TTGCCCAAAC CAGCAGAAAA TCAAAAACCA AAAGGCAAGC CACAACCCAA AGCGAAGACT
    GAAGGCCCCG AGATTAAGAA GGAAGAGGAA ACACTTTCAG CAATATACAC TGTACCAGCC
    ATAGTGGAGG ACAAGAAGAT GCGTAAAGAT AATGTAGTTT ATCTTAATTC ATTGATTACC
    AGTGGTTATA CCAAGAAAGT GGATATCACA TTTATCCCAC AGAGGATCTG GAGTCCTGAA
    GCCACGACAG CAGAGCTGAT CAGAATGAGG GAACTGCGCC GGGAGAGGTT GACATATGAG
    GTAGATTTTG ATGACTTCAT GATGCCCTTT CAGAAGCACA TCACAGAGAG AGCACAAGCA
    GTGGAAGCTA GCTTCAAGAT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92215
    Clone ID Related Accession (Same CDS sequence) XM_015473953.1 , XM_015465856.1 , XM_015473953.2
    Accession Version XM_015473953.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ankyrin repeat and EF-hand domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104360.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCTTTAG CAGATAAGAG ACTCGAGAAC CTGCAGATCT ACAAAGTTCT TCAGTGTGTT 
    CGGAACAAAG ACAAGAAGCA GATAGAGAAG TTGACCAGGC TCGGATATCC TGAACTCATC
    AATTTCACAG ACCCAGTCAA TGGAAACAGT GCTCTGCACT TAGCCTCAGT TTCCAATGAC
    ATCGACATGG TCAGCTTTCT CCTGAGCCTT GGTGCTCACC CTGATGTGCA AGACAAAATG
    GGCTGTACTC CGACTATGAG GGCTGCAGAA CTGGGCCACG AATTGTCCAT GGAAATATTA
    GCCAAAGCAA AAGCCGATAT GACTGTTGTT GATAATGAAG GAAAAGGTAT TTTGTTTTAC
    TGCATTTTAC CTACTAAACG GCATTATCGG TGTGCACTGA TTGCCTTGGA ACATGGTGCG
    GATGTCAACA ATTGTACCTG TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGAAGCA
    CATGATGTTA AGGATGTGTG CCTGACGTTT TTGGAAAAAG GAGCCAATCC AAATGCTATC
    AACACAATAT TGAAGCTTCT TTTTGCATAC AACGGAGACA TGGGGTTGAT TGCAATGAAT
    GGCAACACAC CACTTCATTA TGCTGCCATG GGTGGTTTCG CAGATTGCTG TAAATATATA
    GCTCAGCGAG GATGTGACCT AAAATGGAAG AATCTGGAGC ATAAAACGCC CAGGAACTTG
    GCTAAGGAAG GCGGCTTCAA GGCAGCCAGC AAAGAAATAC GGCGGGCGGA GCAAATCGCT
    AATAAACTGG CCAGGCCAGG GGTCAAAAAT CCGAATCCGC ACTTGTTCCT GAGACTTCAC
    GATTGGTCAG TAGAACATGA GACTTTCCTT CGGGAAGCCT TCTCGTTTGT GGACAGGGGC
    GATGGGACAG TCACCAAGGA GGACTTTGTG CTGACCCTGG AGGAGAGGCA AGATTTTGTG
    AACTCAGAGC AGCTGGCTGC TATAGCGCAA CTTCACGAAA AAGTCCGGGG TGGAGGGGTC
    AATATTAACG AGTTCTTTAA AGGGACCAGA TATTTAAGTA AGTCTTATGT CTTGGGATCC
    TATGGGCCTA AGAAAAAGAA AAAAGGGATG GCCAAAAGAG CCAGAGTGGG CAAGTTTGTT
    TTCCCCCTCC CCATCTGTAT CATCCCCGAC CACGTGTTTC CACGCCGCAG TGATGGTGGG
    CCGCCCTATT ACATGATCGA AACCTACAAG AATGTAACTG ATTGCAACCG CTTTAACAGA
    GATCACCCCC CAGAACATCC CATTCAGGAT GACTCTGCTT GGTATATTGA TGATCCAGGG
    AAGGTCTTTT CAAACATTAA TTTTATCACC AAGGCGGGAG ACCTGGCTTC TCTGAAAAAG
    GCTTTTGAAT CAGGAATACC TGTGGATATG AAGGATAATT ACTACAAGAC ACCGCTAATG
    ACTGCATGTG CCACTGGAAA CATTGATGCA GTCAAGTTTC TTCTTGAAAA AGGGGCTAAT
    GTTAATGCAA CAGATAATTT TCTGTGGACT CCACTCCATT TTGCATGCCA TGCTGGCCAA
    CAGGATATTG TTGAGCTTCT TGTTAAATCT GGAGCCGTGG TAGATGCCCT CTCAATCAAC
    AACTCAACTC CCTTAAGTAG AGCCATTGAG AGCTGCAGAC TGGATACTGT AAAATACCTA
    CTTGACATCG GTGCTAAATT CCAGTTGGAG AATAGGAAAG GGCATAGTGC CATGGATGTT
    GCAAAAGCTT ATGCTGACAG TAGAATAATT GGTTTGATTA AAGAAAAGCT GGATAACTTG
    CCCAAACCAG CAGAAAATCA AAAACCAAAA GGCAAGCCAC AACCCAAAGC GAAGACTGAA
    GGCCCCGAGA TTAAGAAGGA AGAGGAAACA CTTTCAGCAA TATACACTGT ACCAGCCATA
    GTGGAGGACA AGAAGATGCG TAAAGATAAT GTAGTTTATC TTAATTCATT GATTACCAGT
    GGTTATACCA AGAAAGTGGA TATCACATTT ATCCCACAGA GGATCTGGAG TCCTGAAGCC
    ACGACAGCAG AGCTGATCAG AATGAGGGAA CTGCGCCGGG AGAGGTTGAC ATATGAGGTA
    GATTTTGATG ACTTCATGAT GCCCTTTCAG AAGCACATCA CAGAGAGAGC ACAAGCAGTG
    GAAGCTAGCT TCAAGATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329439.1
    CDS360..2540
    Translation

    Target ORF information:

    RefSeq Version XM_015473953.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473953.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCTTTAG CAGATAAGAG ACTCGAGAAC CTGCAGATCT ACAAAGTTCT TCAGTGTGTT 
    CGGAACAAAG ACAAGAAGCA GATAGAGAAG TTGACCAGGC TCGGATATCC TGAACTCATC
    AATTTCACAG ACCCAGTCAA TGGAAACAGT GCTCTGCACT TAGCCTCAGT TTCCAATGAC
    ATCGACATGG TCAGCTTTCT CCTGAGCCTT GGTGCTCACC CTGATGTGCA AGACAAAATG
    GGCTGTACTC CGACTATGAG GGCTGCAGAA CTGGGCCACG AATTGTCCAT GGAAATATTA
    GCCAAAGCAA AAGCCGATAT GACTGTTGTT GATAATGAAG GAAAAGGTAT TTTGTTTTAC
    TGCATTTTAC CTACTAAACG GCATTATCGG TGTGCACTGA TTGCCTTGGA ACATGGTGCG
    GATGTCAACA ATTGTACCTG TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGAAGCA
    CATGATGTTA AGGATGTGTG CCTGACGTTT TTGGAAAAAG GAGCCAATCC AAATGCTATC
    AACACAATAT TGAAGCTTCT TTTTGCATAC AACGGAGACA TGGGGTTGAT TGCAATGAAT
    GGCAACACAC CACTTCATTA TGCTGCCATG GGTGGTTTCG CAGATTGCTG TAAATATATA
    GCTCAGCGAG GATGTGACCT AAAATGGAAG AATCTGGAGC ATAAAACGCC CAGGAACTTG
    GCTAAGGAAG GCGGCTTCAA GGCAGCCAGC AAAGAAATAC GGCGGGCGGA GCAAATCGCT
    AATAAACTGG CCAGGCCAGG GGTCAAAAAT CCGAATCCGC ACTTGTTCCT GAGACTTCAC
    GATTGGTCAG TAGAACATGA GACTTTCCTT CGGGAAGCCT TCTCGTTTGT GGACAGGGGC
    GATGGGACAG TCACCAAGGA GGACTTTGTG CTGACCCTGG AGGAGAGGCA AGATTTTGTG
    AACTCAGAGC AGCTGGCTGC TATAGCGCAA CTTCACGAAA AAGTCCGGGG TGGAGGGGTC
    AATATTAACG AGTTCTTTAA AGGGACCAGA TATTTAAGTA AGTCTTATGT CTTGGGATCC
    TATGGGCCTA AGAAAAAGAA AAAAGGGATG GCCAAAAGAG CCAGAGTGGG CAAGTTTGTT
    TTCCCCCTCC CCATCTGTAT CATCCCCGAC CACGTGTTTC CACGCCGCAG TGATGGTGGG
    CCGCCCTATT ACATGATCGA AACCTACAAG AATGTAACTG ATTGCAACCG CTTTAACAGA
    GATCACCCCC CAGAACATCC CATTCAGGAT GACTCTGCTT GGTATATTGA TGATCCAGGG
    AAGGTCTTTT CAAACATTAA TTTTATCACC AAGGCGGGAG ACCTGGCTTC TCTGAAAAAG
    GCTTTTGAAT CAGGAATACC TGTGGATATG AAGGATAATT ACTACAAGAC ACCGCTAATG
    ACTGCATGTG CCACTGGAAA CATTGATGCA GTCAAGTTTC TTCTTGAAAA AGGGGCTAAT
    GTTAATGCAA CAGATAATTT TCTGTGGACT CCACTCCATT TTGCATGCCA TGCTGGCCAA
    CAGGATATTG TTGAGCTTCT TGTTAAATCT GGAGCCGTGG TAGATGCCCT CTCAATCAAC
    AACTCAACTC CCTTAAGTAG AGCCATTGAG AGCTGCAGAC TGGATACTGT AAAATACCTA
    CTTGACATCG GTGCTAAATT CCAGTTGGAG AATAGGAAAG GGCATAGTGC CATGGATGTT
    GCAAAAGCTT ATGCTGACAG TAGAATAATT GGTTTGATTA AAGAAAAGCT GGATAACTTG
    CCCAAACCAG CAGAAAATCA AAAACCAAAA GGCAAGCCAC AACCCAAAGC GAAGACTGAA
    GGCCCCGAGA TTAAGAAGGA AGAGGAAACA CTTTCAGCAA TATACACTGT ACCAGCCATA
    GTGGAGGACA AGAAGATGCG TAAAGATAAT GTAGTTTATC TTAATTCATT GATTACCAGT
    GGTTATACCA AGAAAGTGGA TATCACATTT ATCCCACAGA GGATCTGGAG TCCTGAAGCC
    ACGACAGCAG AGCTGATCAG AATGAGGGAA CTGCGCCGGG AGAGGTTGAC ATATGAGGTA
    GATTTTGATG ACTTCATGAT GCCCTTTCAG AAGCACATCA CAGAGAGAGC ACAAGCAGTG
    GAAGCTAGCT TCAAGATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92215
    Clone ID Related Accession (Same CDS sequence) XM_015473953.1 , XM_015465856.1 , XM_015473953.2
    Accession Version XM_015465856.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ankyrin repeat and EF-hand domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646117.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCTTTAG CAGATAAGAG ACTCGAGAAC CTGCAGATCT ACAAAGTTCT TCAGTGTGTT 
    CGGAACAAAG ACAAGAAGCA GATAGAGAAG TTGACCAGGC TCGGATATCC TGAACTCATC
    AATTTCACAG ACCCAGTCAA TGGAAACAGT GCTCTGCACT TAGCCTCAGT TTCCAATGAC
    ATCGACATGG TCAGCTTTCT CCTGAGCCTT GGTGCTCACC CTGATGTGCA AGACAAAATG
    GGCTGTACTC CGACTATGAG GGCTGCAGAA CTGGGCCACG AATTGTCCAT GGAAATATTA
    GCCAAAGCAA AAGCCGATAT GACTGTTGTT GATAATGAAG GAAAAGGTAT TTTGTTTTAC
    TGCATTTTAC CTACTAAACG GCATTATCGG TGTGCACTGA TTGCCTTGGA ACATGGTGCG
    GATGTCAACA ATTGTACCTG TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGAAGCA
    CATGATGTTA AGGATGTGTG CCTGACGTTT TTGGAAAAAG GAGCCAATCC AAATGCTATC
    AACACAATAT TGAAGCTTCT TTTTGCATAC AACGGAGACA TGGGGTTGAT TGCAATGAAT
    GGCAACACAC CACTTCATTA TGCTGCCATG GGTGGTTTCG CAGATTGCTG TAAATATATA
    GCTCAGCGAG GATGTGACCT AAAATGGAAG AATCTGGAGC ATAAAACGCC CAGGAACTTG
    GCTAAGGAAG GCGGCTTCAA GGCAGCCAGC AAAGAAATAC GGCGGGCGGA GCAAATCGCT
    AATAAACTGG CCAGGCCAGG GGTCAAAAAT CCGAATCCGC ACTTGTTCCT GAGACTTCAC
    GATTGGTCAG TAGAACATGA GACTTTCCTT CGGGAAGCCT TCTCGTTTGT GGACAGGGGC
    GATGGGACAG TCACCAAGGA GGACTTTGTG CTGACCCTGG AGGAGAGGCA AGATTTTGTG
    AACTCAGAGC AGCTGGCTGC TATAGCGCAA CTTCACGAAA AAGTCCGGGG TGGAGGGGTC
    AATATTAACG AGTTCTTTAA AGGGACCAGA TATTTAAGTA AGTCTTATGT CTTGGGATCC
    TATGGGCCTA AGAAAAAGAA AAAAGGGATG GCCAAAAGAG CCAGAGTGGG CAAGTTTGTT
    TTCCCCCTCC CCATCTGTAT CATCCCCGAC CACGTGTTTC CACGCCGCAG TGATGGTGGG
    CCGCCCTATT ACATGATCGA AACCTACAAG AATGTAACTG ATTGCAACCG CTTTAACAGA
    GATCACCCCC CAGAACATCC CATTCAGGAT GACTCTGCTT GGTATATTGA TGATCCAGGG
    AAGGTCTTTT CAAACATTAA TTTTATCACC AAGGCGGGAG ACCTGGCTTC TCTGAAAAAG
    GCTTTTGAAT CAGGAATACC TGTGGATATG AAGGATAATT ACTACAAGAC ACCGCTAATG
    ACTGCATGTG CCACTGGAAA CATTGATGCA GTCAAGTTTC TTCTTGAAAA AGGGGCTAAT
    GTTAATGCAA CAGATAATTT TCTGTGGACT CCACTCCATT TTGCATGCCA TGCTGGCCAA
    CAGGATATTG TTGAGCTTCT TGTTAAATCT GGAGCCGTGG TAGATGCCCT CTCAATCAAC
    AACTCAACTC CCTTAAGTAG AGCCATTGAG AGCTGCAGAC TGGATACTGT AAAATACCTA
    CTTGACATCG GTGCTAAATT CCAGTTGGAG AATAGGAAAG GGCATAGTGC CATGGATGTT
    GCAAAAGCTT ATGCTGACAG TAGAATAATT GGTTTGATTA AAGAAAAGCT GGATAACTTG
    CCCAAACCAG CAGAAAATCA AAAACCAAAA GGCAAGCCAC AACCCAAAGC GAAGACTGAA
    GGCCCCGAGA TTAAGAAGGA AGAGGAAACA CTTTCAGCAA TATACACTGT ACCAGCCATA
    GTGGAGGACA AGAAGATGCG TAAAGATAAT GTAGTTTATC TTAATTCATT GATTACCAGT
    GGTTATACCA AGAAAGTGGA TATCACATTT ATCCCACAGA GGATCTGGAG TCCTGAAGCC
    ACGACAGCAG AGCTGATCAG AATGAGGGAA CTGCGCCGGG AGAGGTTGAC ATATGAGGTA
    GATTTTGATG ACTTCATGAT GCCCTTTCAG AAGCACATCA CAGAGAGAGC ACAAGCAGTG
    GAAGCTAGCT TCAAGATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321342.1
    CDS360..2540
    Translation

    Target ORF information:

    RefSeq Version XM_015465856.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465856.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCTTTAG CAGATAAGAG ACTCGAGAAC CTGCAGATCT ACAAAGTTCT TCAGTGTGTT 
    CGGAACAAAG ACAAGAAGCA GATAGAGAAG TTGACCAGGC TCGGATATCC TGAACTCATC
    AATTTCACAG ACCCAGTCAA TGGAAACAGT GCTCTGCACT TAGCCTCAGT TTCCAATGAC
    ATCGACATGG TCAGCTTTCT CCTGAGCCTT GGTGCTCACC CTGATGTGCA AGACAAAATG
    GGCTGTACTC CGACTATGAG GGCTGCAGAA CTGGGCCACG AATTGTCCAT GGAAATATTA
    GCCAAAGCAA AAGCCGATAT GACTGTTGTT GATAATGAAG GAAAAGGTAT TTTGTTTTAC
    TGCATTTTAC CTACTAAACG GCATTATCGG TGTGCACTGA TTGCCTTGGA ACATGGTGCG
    GATGTCAACA ATTGTACCTG TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGAAGCA
    CATGATGTTA AGGATGTGTG CCTGACGTTT TTGGAAAAAG GAGCCAATCC AAATGCTATC
    AACACAATAT TGAAGCTTCT TTTTGCATAC AACGGAGACA TGGGGTTGAT TGCAATGAAT
    GGCAACACAC CACTTCATTA TGCTGCCATG GGTGGTTTCG CAGATTGCTG TAAATATATA
    GCTCAGCGAG GATGTGACCT AAAATGGAAG AATCTGGAGC ATAAAACGCC CAGGAACTTG
    GCTAAGGAAG GCGGCTTCAA GGCAGCCAGC AAAGAAATAC GGCGGGCGGA GCAAATCGCT
    AATAAACTGG CCAGGCCAGG GGTCAAAAAT CCGAATCCGC ACTTGTTCCT GAGACTTCAC
    GATTGGTCAG TAGAACATGA GACTTTCCTT CGGGAAGCCT TCTCGTTTGT GGACAGGGGC
    GATGGGACAG TCACCAAGGA GGACTTTGTG CTGACCCTGG AGGAGAGGCA AGATTTTGTG
    AACTCAGAGC AGCTGGCTGC TATAGCGCAA CTTCACGAAA AAGTCCGGGG TGGAGGGGTC
    AATATTAACG AGTTCTTTAA AGGGACCAGA TATTTAAGTA AGTCTTATGT CTTGGGATCC
    TATGGGCCTA AGAAAAAGAA AAAAGGGATG GCCAAAAGAG CCAGAGTGGG CAAGTTTGTT
    TTCCCCCTCC CCATCTGTAT CATCCCCGAC CACGTGTTTC CACGCCGCAG TGATGGTGGG
    CCGCCCTATT ACATGATCGA AACCTACAAG AATGTAACTG ATTGCAACCG CTTTAACAGA
    GATCACCCCC CAGAACATCC CATTCAGGAT GACTCTGCTT GGTATATTGA TGATCCAGGG
    AAGGTCTTTT CAAACATTAA TTTTATCACC AAGGCGGGAG ACCTGGCTTC TCTGAAAAAG
    GCTTTTGAAT CAGGAATACC TGTGGATATG AAGGATAATT ACTACAAGAC ACCGCTAATG
    ACTGCATGTG CCACTGGAAA CATTGATGCA GTCAAGTTTC TTCTTGAAAA AGGGGCTAAT
    GTTAATGCAA CAGATAATTT TCTGTGGACT CCACTCCATT TTGCATGCCA TGCTGGCCAA
    CAGGATATTG TTGAGCTTCT TGTTAAATCT GGAGCCGTGG TAGATGCCCT CTCAATCAAC
    AACTCAACTC CCTTAAGTAG AGCCATTGAG AGCTGCAGAC TGGATACTGT AAAATACCTA
    CTTGACATCG GTGCTAAATT CCAGTTGGAG AATAGGAAAG GGCATAGTGC CATGGATGTT
    GCAAAAGCTT ATGCTGACAG TAGAATAATT GGTTTGATTA AAGAAAAGCT GGATAACTTG
    CCCAAACCAG CAGAAAATCA AAAACCAAAA GGCAAGCCAC AACCCAAAGC GAAGACTGAA
    GGCCCCGAGA TTAAGAAGGA AGAGGAAACA CTTTCAGCAA TATACACTGT ACCAGCCATA
    GTGGAGGACA AGAAGATGCG TAAAGATAAT GTAGTTTATC TTAATTCATT GATTACCAGT
    GGTTATACCA AGAAAGTGGA TATCACATTT ATCCCACAGA GGATCTGGAG TCCTGAAGCC
    ACGACAGCAG AGCTGATCAG AATGAGGGAA CTGCGCCGGG AGAGGTTGAC ATATGAGGTA
    GATTTTGATG ACTTCATGAT GCCCTTTCAG AAGCACATCA CAGAGAGAGC ACAAGCAGTG
    GAAGCTAGCT TCAAGATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92215
    Clone ID Related Accession (Same CDS sequence) XM_015473953.1 , XM_015465856.1 , XM_015473953.2
    Accession Version XM_015473953.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ankyrin repeat and EF-hand domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473953.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCTTTAG CAGATAAGAG ACTCGAGAAC CTGCAGATCT ACAAAGTTCT TCAGTGTGTT 
    CGGAACAAAG ACAAGAAGCA GATAGAGAAG TTGACCAGGC TCGGATATCC TGAACTCATC
    AATTTCACAG ACCCAGTCAA TGGAAACAGT GCTCTGCACT TAGCCTCAGT TTCCAATGAC
    ATCGACATGG TCAGCTTTCT CCTGAGCCTT GGTGCTCACC CTGATGTGCA AGACAAAATG
    GGCTGTACTC CGACTATGAG GGCTGCAGAA CTGGGCCACG AATTGTCCAT GGAAATATTA
    GCCAAAGCAA AAGCCGATAT GACTGTTGTT GATAATGAAG GAAAAGGTAT TTTGTTTTAC
    TGCATTTTAC CTACTAAACG GCATTATCGG TGTGCACTGA TTGCCTTGGA ACATGGTGCG
    GATGTCAACA ATTGTACCTG TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGAAGCA
    CATGATGTTA AGGATGTGTG CCTGACGTTT TTGGAAAAAG GAGCCAATCC AAATGCTATC
    AACACAATAT TGAAGCTTCT TTTTGCATAC AACGGAGACA TGGGGTTGAT TGCAATGAAT
    GGCAACACAC CACTTCATTA TGCTGCCATG GGTGGTTTCG CAGATTGCTG TAAATATATA
    GCTCAGCGAG GATGTGACCT AAAATGGAAG AATCTGGAGC ATAAAACGCC CAGGAACTTG
    GCTAAGGAAG GCGGCTTCAA GGCAGCCAGC AAAGAAATAC GGCGGGCGGA GCAAATCGCT
    AATAAACTGG CCAGGCCAGG GGTCAAAAAT CCGAATCCGC ACTTGTTCCT GAGACTTCAC
    GATTGGTCAG TAGAACATGA GACTTTCCTT CGGGAAGCCT TCTCGTTTGT GGACAGGGGC
    GATGGGACAG TCACCAAGGA GGACTTTGTG CTGACCCTGG AGGAGAGGCA AGATTTTGTG
    AACTCAGAGC AGCTGGCTGC TATAGCGCAA CTTCACGAAA AAGTCCGGGG TGGAGGGGTC
    AATATTAACG AGTTCTTTAA AGGGACCAGA TATTTAAGTA AGTCTTATGT CTTGGGATCC
    TATGGGCCTA AGAAAAAGAA AAAAGGGATG GCCAAAAGAG CCAGAGTGGG CAAGTTTGTT
    TTCCCCCTCC CCATCTGTAT CATCCCCGAC CACGTGTTTC CACGCCGCAG TGATGGTGGG
    CCGCCCTATT ACATGATCGA AACCTACAAG AATGTAACTG ATTGCAACCG CTTTAACAGA
    GATCACCCCC CAGAACATCC CATTCAGGAT GACTCTGCTT GGTATATTGA TGATCCAGGG
    AAGGTCTTTT CAAACATTAA TTTTATCACC AAGGCGGGAG ACCTGGCTTC TCTGAAAAAG
    GCTTTTGAAT CAGGAATACC TGTGGATATG AAGGATAATT ACTACAAGAC ACCGCTAATG
    ACTGCATGTG CCACTGGAAA CATTGATGCA GTCAAGTTTC TTCTTGAAAA AGGGGCTAAT
    GTTAATGCAA CAGATAATTT TCTGTGGACT CCACTCCATT TTGCATGCCA TGCTGGCCAA
    CAGGATATTG TTGAGCTTCT TGTTAAATCT GGAGCCGTGG TAGATGCCCT CTCAATCAAC
    AACTCAACTC CCTTAAGTAG AGCCATTGAG AGCTGCAGAC TGGATACTGT AAAATACCTA
    CTTGACATCG GTGCTAAATT CCAGTTGGAG AATAGGAAAG GGCATAGTGC CATGGATGTT
    GCAAAAGCTT ATGCTGACAG TAGAATAATT GGTTTGATTA AAGAAAAGCT GGATAACTTG
    CCCAAACCAG CAGAAAATCA AAAACCAAAA GGCAAGCCAC AACCCAAAGC GAAGACTGAA
    GGCCCCGAGA TTAAGAAGGA AGAGGAAACA CTTTCAGCAA TATACACTGT ACCAGCCATA
    GTGGAGGACA AGAAGATGCG TAAAGATAAT GTAGTTTATC TTAATTCATT GATTACCAGT
    GGTTATACCA AGAAAGTGGA TATCACATTT ATCCCACAGA GGATCTGGAG TCCTGAAGCC
    ACGACAGCAG AGCTGATCAG AATGAGGGAA CTGCGCCGGG AGAGGTTGAC ATATGAGGTA
    GATTTTGATG ACTTCATGAT GCCCTTTCAG AAGCACATCA CAGAGAGAGC ACAAGCAGTG
    GAAGCTAGCT TCAAGATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329439.1
    CDS294..2474
    Translation

    Target ORF information:

    RefSeq Version XM_015473953.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473953.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCTTTAG CAGATAAGAG ACTCGAGAAC CTGCAGATCT ACAAAGTTCT TCAGTGTGTT 
    CGGAACAAAG ACAAGAAGCA GATAGAGAAG TTGACCAGGC TCGGATATCC TGAACTCATC
    AATTTCACAG ACCCAGTCAA TGGAAACAGT GCTCTGCACT TAGCCTCAGT TTCCAATGAC
    ATCGACATGG TCAGCTTTCT CCTGAGCCTT GGTGCTCACC CTGATGTGCA AGACAAAATG
    GGCTGTACTC CGACTATGAG GGCTGCAGAA CTGGGCCACG AATTGTCCAT GGAAATATTA
    GCCAAAGCAA AAGCCGATAT GACTGTTGTT GATAATGAAG GAAAAGGTAT TTTGTTTTAC
    TGCATTTTAC CTACTAAACG GCATTATCGG TGTGCACTGA TTGCCTTGGA ACATGGTGCG
    GATGTCAACA ATTGTACCTG TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGAAGCA
    CATGATGTTA AGGATGTGTG CCTGACGTTT TTGGAAAAAG GAGCCAATCC AAATGCTATC
    AACACAATAT TGAAGCTTCT TTTTGCATAC AACGGAGACA TGGGGTTGAT TGCAATGAAT
    GGCAACACAC CACTTCATTA TGCTGCCATG GGTGGTTTCG CAGATTGCTG TAAATATATA
    GCTCAGCGAG GATGTGACCT AAAATGGAAG AATCTGGAGC ATAAAACGCC CAGGAACTTG
    GCTAAGGAAG GCGGCTTCAA GGCAGCCAGC AAAGAAATAC GGCGGGCGGA GCAAATCGCT
    AATAAACTGG CCAGGCCAGG GGTCAAAAAT CCGAATCCGC ACTTGTTCCT GAGACTTCAC
    GATTGGTCAG TAGAACATGA GACTTTCCTT CGGGAAGCCT TCTCGTTTGT GGACAGGGGC
    GATGGGACAG TCACCAAGGA GGACTTTGTG CTGACCCTGG AGGAGAGGCA AGATTTTGTG
    AACTCAGAGC AGCTGGCTGC TATAGCGCAA CTTCACGAAA AAGTCCGGGG TGGAGGGGTC
    AATATTAACG AGTTCTTTAA AGGGACCAGA TATTTAAGTA AGTCTTATGT CTTGGGATCC
    TATGGGCCTA AGAAAAAGAA AAAAGGGATG GCCAAAAGAG CCAGAGTGGG CAAGTTTGTT
    TTCCCCCTCC CCATCTGTAT CATCCCCGAC CACGTGTTTC CACGCCGCAG TGATGGTGGG
    CCGCCCTATT ACATGATCGA AACCTACAAG AATGTAACTG ATTGCAACCG CTTTAACAGA
    GATCACCCCC CAGAACATCC CATTCAGGAT GACTCTGCTT GGTATATTGA TGATCCAGGG
    AAGGTCTTTT CAAACATTAA TTTTATCACC AAGGCGGGAG ACCTGGCTTC TCTGAAAAAG
    GCTTTTGAAT CAGGAATACC TGTGGATATG AAGGATAATT ACTACAAGAC ACCGCTAATG
    ACTGCATGTG CCACTGGAAA CATTGATGCA GTCAAGTTTC TTCTTGAAAA AGGGGCTAAT
    GTTAATGCAA CAGATAATTT TCTGTGGACT CCACTCCATT TTGCATGCCA TGCTGGCCAA
    CAGGATATTG TTGAGCTTCT TGTTAAATCT GGAGCCGTGG TAGATGCCCT CTCAATCAAC
    AACTCAACTC CCTTAAGTAG AGCCATTGAG AGCTGCAGAC TGGATACTGT AAAATACCTA
    CTTGACATCG GTGCTAAATT CCAGTTGGAG AATAGGAAAG GGCATAGTGC CATGGATGTT
    GCAAAAGCTT ATGCTGACAG TAGAATAATT GGTTTGATTA AAGAAAAGCT GGATAACTTG
    CCCAAACCAG CAGAAAATCA AAAACCAAAA GGCAAGCCAC AACCCAAAGC GAAGACTGAA
    GGCCCCGAGA TTAAGAAGGA AGAGGAAACA CTTTCAGCAA TATACACTGT ACCAGCCATA
    GTGGAGGACA AGAAGATGCG TAAAGATAAT GTAGTTTATC TTAATTCATT GATTACCAGT
    GGTTATACCA AGAAAGTGGA TATCACATTT ATCCCACAGA GGATCTGGAG TCCTGAAGCC
    ACGACAGCAG AGCTGATCAG AATGAGGGAA CTGCGCCGGG AGAGGTTGAC ATATGAGGTA
    GATTTTGATG ACTTCATGAT GCCCTTTCAG AAGCACATCA CAGAGAGAGC ACAAGCAGTG
    GAAGCTAGCT TCAAGATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.