ATG7 cDNA ORF clone, Bos taurus(cattle)

The following ATG7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATG7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa229211 XM_024983115.1
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa01481 NM_001083795.2
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa01482 NM_001142967.1
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa31765 XM_005223099.3
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa58263 XM_010817935.2
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa58263 XM_010817937.2
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa58263 XM_010817941.2
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa58263 XM_010817939.2
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa58263 XM_010817938.2
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa58263 XM_010817940.2
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa58264 XM_010817936.2
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa58265 XM_010817942.2
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa58268 XM_010817945.2
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa229200 XM_010817940.3
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229200 XM_010817937.3
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229200 XM_024983105.1
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229200 XM_010817939.3
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229200 XM_024983106.1
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229200 XM_010817935.3
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229200 XM_010817938.3
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229200 XM_010817941.3
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229200 XM_024983104.1
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229201 XM_010817936.3
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229202 XM_010817942.3
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229203 XM_024983107.1
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229204 XM_005223099.4
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229205 XM_024983108.1
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229205 XM_024983110.1
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229206 XM_010817945.3
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229207 XM_024983111.1
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229208 XM_024983112.1
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa229209 XM_024983113.1
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa229210 XM_024983114.1
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa94472 XM_015459680.1
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa94473 XM_015459681.1
Latest version!
Bos taurus autophagy related 7 (ATG7), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa229211
    Clone ID Related Accession (Same CDS sequence) XM_024983115.1
    Accession Version XM_024983115.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGACCGA GGATGGTGAA CCTCAGCGAG TGTATGGACC CTAAAAGGTT AGCTGAGTCA 
    TCGGTCGATC TCAACCTCAA GCTGATGTGT TGGAGATTGG TCCCTACTCT GGACTTAGAC
    AAGGTTGTGT CTGCCAAGTG TCTGCTGCTT GGAGCCGGCA CCTTGGGTTG TAACGTAGCC
    CGGACGTTGA TGGGTTGGGG TGTCAGACAC ATCACGTTCG TGGACAATGC CAAGATCTCC
    TACTCTAACC CTGTGAGGCA GCCTCTGTAT GAATTTGAAG ATTGCCTGGC GGGTGGGAAG
    CCCAAGGCCC TGGCAGCCGC AGACCGGCTG CAGAAGATAT TCCCCGGAGT GAACGCCAGA
    GGGTTCAACA TGAGCATCCC CATGCCGGGG CACCCCGTGA ACTTCTCCAG CGTCACCCTG
    GAGCAGGCCC GCAGGGACGT GGAACAGCTG GAGCAGCTCA TTGACAGCCA CGACGTCGTC
    TTCCTGTTGA TGGACACCCG GGAGAGCCGG TGGCTGCCTG CCGTCATCGC CGCGAGCAAG
    AGAAAGCTGG TCATCAATGC TGCCTTGGGA TTTGACACCT TCGTTGTCAT GAGACACGGC
    CTGAAGAAGC CCAGGCATCA GGGAGCTGGG GACTTGTGTC CCAGCTACCC CATGGCGTCT
    GCCGACCTCC TGAGCTCATC GCTGTTTGCC AACATCCCTG GCTATAAACT CGGCTGCTAC
    TTCTGCAACG ATGTGGTGGC CCCAGGGGAT TCAACCAGAG ACCGGACCTT GGACCAGCAG
    TGCACTGTGA GCCGTCCAGG ACTAGCCATG ATCGCAGGAG CCCTGGCGGT GGAGTTGATG
    GTTTCCGTTT TGCAGCATCC AGAAGGGGGC TATGCCATTG CCAGCAGCAG TGACGACCGC
    ATGAATGAGC CGCCAACGTC CCTCGGCCTC GTGCCTCACC AGATACGGGG ATTTCTGTCA
    CGGTTTGATA ACGTCCTTCC TGTCAGCCTG GCCTTTGACA AATGCACGGC TTGTTCTTCC
    AAAGTTCTCG ATCAGTATGA ACGAGAAGGA TTTAATTTCC TGGCGAAGGT GTTTAATTCT
    TCGCACTCCT TCTTAGAGGA CTTGACCGGT CTTACATTGC TGCATCAAGA GACCCAGGCT
    GCTGAGAAGA CTCTGGTACT CCAAACCTGT AGGTTTCCCG CCCCCACACC AAGCAGTTCT
    CTAATTCTCC ACAGACACCA GCCGGGTGTC CTGCAGTTGA GTTCCATTCT GACACCCCGT
    CTGCCTGGAG GTTCCATCAG ATCCCACAGC TGTGCTGGGT CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838883.1
    CDS167..1471
    Translation

    Target ORF information:

    RefSeq Version XM_024983115.1
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983115.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGACCGA GGATGGTGAA CCTCAGCGAG TGTATGGACC CTAAAAGGTT AGCTGAGTCA 
    TCGGTCGATC TCAACCTCAA GCTGATGTGT TGGAGATTGG TCCCTACTCT GGACTTAGAC
    AAGGTTGTGT CTGCCAAGTG TCTGCTGCTT GGAGCCGGCA CCTTGGGTTG TAACGTAGCC
    CGGACGTTGA TGGGTTGGGG TGTCAGACAC ATCACGTTCG TGGACAATGC CAAGATCTCC
    TACTCTAACC CTGTGAGGCA GCCTCTGTAT GAATTTGAAG ATTGCCTGGC GGGTGGGAAG
    CCCAAGGCCC TGGCAGCCGC AGACCGGCTG CAGAAGATAT TCCCCGGAGT GAACGCCAGA
    GGGTTCAACA TGAGCATCCC CATGCCGGGG CACCCCGTGA ACTTCTCCAG CGTCACCCTG
    GAGCAGGCCC GCAGGGACGT GGAACAGCTG GAGCAGCTCA TTGACAGCCA CGACGTCGTC
    TTCCTGTTGA TGGACACCCG GGAGAGCCGG TGGCTGCCTG CCGTCATCGC CGCGAGCAAG
    AGAAAGCTGG TCATCAATGC TGCCTTGGGA TTTGACACCT TCGTTGTCAT GAGACACGGC
    CTGAAGAAGC CCAGGCATCA GGGAGCTGGG GACTTGTGTC CCAGCTACCC CATGGCGTCT
    GCCGACCTCC TGAGCTCATC GCTGTTTGCC AACATCCCTG GCTATAAACT CGGCTGCTAC
    TTCTGCAACG ATGTGGTGGC CCCAGGGGAT TCAACCAGAG ACCGGACCTT GGACCAGCAG
    TGCACTGTGA GCCGTCCAGG ACTAGCCATG ATCGCAGGAG CCCTGGCGGT GGAGTTGATG
    GTTTCCGTTT TGCAGCATCC AGAAGGGGGC TATGCCATTG CCAGCAGCAG TGACGACCGC
    ATGAATGAGC CGCCAACGTC CCTCGGCCTC GTGCCTCACC AGATACGGGG ATTTCTGTCA
    CGGTTTGATA ACGTCCTTCC TGTCAGCCTG GCCTTTGACA AATGCACGGC TTGTTCTTCC
    AAAGTTCTCG ATCAGTATGA ACGAGAAGGA TTTAATTTCC TGGCGAAGGT GTTTAATTCT
    TCGCACTCCT TCTTAGAGGA CTTGACCGGT CTTACATTGC TGCATCAAGA GACCCAGGCT
    GCTGAGAAGA CTCTGGTACT CCAAACCTGT AGGTTTCCCG CCCCCACACC AAGCAGTTCT
    CTAATTCTCC ACAGACACCA GCCGGGTGTC CTGCAGTTGA GTTCCATTCT GACACCCCGT
    CTGCCTGGAG GTTCCATCAG ATCCCACAGC TGTGCTGGGT CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01481
    Clone ID Related Accession (Same CDS sequence) NM_001083795.2
    Accession Version NM_001083795.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2100bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC134445.1. On Jan 1, 2009 this sequence version replaced NM_001083795.1. Transcript Variant: This variant (2) uses an alternate splice pattern in the 3' coding region, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC134445.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145420 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGG TGTATAAACA AGAGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001077264.1
    CDS60..2159
    Translation

    Target ORF information:

    RefSeq Version NM_001083795.2
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083795.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGG TGTATAAACA AGAGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01482
    Clone ID Related Accession (Same CDS sequence) NM_001142967.1
    Accession Version NM_001142967.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC134445.1, CV979774.1 and EE378507.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145419 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA TCTGGGACAT GAGTGACGAC
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001136439.1
    CDS60..2171
    Translation

    Target ORF information:

    RefSeq Version NM_001142967.1
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001142967.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA TCTGGGACAT GAGTGACGAC
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31765
    Clone ID Related Accession (Same CDS sequence) XM_005223099.3
    Accession Version XM_005223099.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2154bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104545.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223099.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA ATAGAGCGAT TCTGGACACA
    GACTACCCTG TCCCTCCAGT GAAAAGGCAA GCACCGAATG GACAGTCGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223156.1
    CDS90..2243
    Translation

    Target ORF information:

    RefSeq Version XM_005223099.3
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223099.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA ATAGAGCGAT TCTGGACACA
    GACTACCCTG TCCCTCCAGT GAAAAGGCAA GCACCGAATG GACAGTCGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58263
    Clone ID Related Accession (Same CDS sequence) XM_010817937.2 , XM_010817935.2 , XM_010817941.2 , XM_010817939.2 , XM_010817940.2 , XM_010817938.2
    Accession Version XM_010817937.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104545.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817937.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816239.1
    CDS393..2630
    Translation

    Target ORF information:

    RefSeq Version XM_010817937.2
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817937.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58263
    Clone ID Related Accession (Same CDS sequence) XM_010817937.2 , XM_010817935.2 , XM_010817941.2 , XM_010817939.2 , XM_010817940.2 , XM_010817938.2
    Accession Version XM_010817935.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104545.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817935.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816237.1
    CDS90..2327
    Translation

    Target ORF information:

    RefSeq Version XM_010817935.2
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817935.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58263
    Clone ID Related Accession (Same CDS sequence) XM_010817937.2 , XM_010817935.2 , XM_010817941.2 , XM_010817939.2 , XM_010817940.2 , XM_010817938.2
    Accession Version XM_010817941.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104545.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817941.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816243.1
    CDS41..2278
    Translation

    Target ORF information:

    RefSeq Version XM_010817941.2
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817941.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58263
    Clone ID Related Accession (Same CDS sequence) XM_010817937.2 , XM_010817935.2 , XM_010817941.2 , XM_010817939.2 , XM_010817940.2 , XM_010817938.2
    Accession Version XM_010817939.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104545.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817939.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816241.1
    CDS39..2276
    Translation

    Target ORF information:

    RefSeq Version XM_010817939.2
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817939.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58263
    Clone ID Related Accession (Same CDS sequence) XM_010817937.2 , XM_010817935.2 , XM_010817941.2 , XM_010817939.2 , XM_010817940.2 , XM_010817938.2
    Accession Version XM_010817940.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104545.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817940.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816242.1
    CDS105..2342
    Translation

    Target ORF information:

    RefSeq Version XM_010817940.2
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817940.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58263
    Clone ID Related Accession (Same CDS sequence) XM_010817937.2 , XM_010817935.2 , XM_010817941.2 , XM_010817939.2 , XM_010817940.2 , XM_010817938.2
    Accession Version XM_010817938.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104545.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817938.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816240.1
    CDS74..2311
    Translation

    Target ORF information:

    RefSeq Version XM_010817938.2
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817938.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58264
    Clone ID Related Accession (Same CDS sequence) XM_010817936.2
    Accession Version XM_010817936.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104545.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817936.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGG GGAGTTCTTT ACCGTCTGAG
    CTACCAGGGA ATCCCTCAAG ATGGATGAAT GAGATTAAAT CAACACATCC CCCCGACTCA
    GCCCTCTCCC ACAGTAACCT AGGTAAAGAA GCTCTTAGGA AAACGTGGCC TGTCATAGCA
    TTTCCTCAAG TAACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816238.1
    CDS90..2327
    Translation

    Target ORF information:

    RefSeq Version XM_010817936.2
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817936.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGG GGAGTTCTTT ACCGTCTGAG
    CTACCAGGGA ATCCCTCAAG ATGGATGAAT GAGATTAAAT CAACACATCC CCCCGACTCA
    GCCCTCTCCC ACAGTAACCT AGGTAAAGAA GCTCTTAGGA AAACGTGGCC TGTCATAGCA
    TTTCCTCAAG TAACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58265
    Clone ID Related Accession (Same CDS sequence) XM_010817942.2
    Accession Version XM_010817942.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2172bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104545.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817942.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA CACCAGCCGG GTGTCCTGCA
    GTTGAGTTCC ATTCTGACAC CCCGTCTGCC TGGAGGTTCC ATCAGATCCC ACAGGTAAGG
    GCTCAGGCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816244.1
    CDS90..2261
    Translation

    Target ORF information:

    RefSeq Version XM_010817942.2
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817942.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA CACCAGCCGG GTGTCCTGCA
    GTTGAGTTCC ATTCTGACAC CCCGTCTGCC TGGAGGTTCC ATCAGATCCC ACAGGTAAGG
    GCTCAGGCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58268
    Clone ID Related Accession (Same CDS sequence) XM_010817945.2
    Accession Version XM_010817945.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2106bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104545.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817945.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA CGTACGACGA CCATAGGAAA
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816247.1
    CDS90..2195
    Translation

    Target ORF information:

    RefSeq Version XM_010817945.2
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817945.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA CGTACGACGA CCATAGGAAA
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229200
    Clone ID Related Accession (Same CDS sequence) XM_010817941.3 , XM_010817939.3 , XM_010817940.3 , XM_010817938.3 , XM_024983104.1 , XM_024983105.1 , XM_024983106.1 , XM_010817937.3 , XM_010817935.3
    Accession Version XM_010817941.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817941.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816243.1
    CDS56..2293
    Translation

    Target ORF information:

    RefSeq Version XM_010817941.3
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817941.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229200
    Clone ID Related Accession (Same CDS sequence) XM_010817941.3 , XM_010817939.3 , XM_010817940.3 , XM_010817938.3 , XM_024983104.1 , XM_024983105.1 , XM_024983106.1 , XM_010817937.3 , XM_010817935.3
    Accession Version XM_010817939.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817939.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816241.1
    CDS68..2305
    Translation

    Target ORF information:

    RefSeq Version XM_010817939.3
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817939.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229200
    Clone ID Related Accession (Same CDS sequence) XM_010817941.3 , XM_010817939.3 , XM_010817940.3 , XM_010817938.3 , XM_024983104.1 , XM_024983105.1 , XM_024983106.1 , XM_010817937.3 , XM_010817935.3
    Accession Version XM_010817940.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817940.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816242.1
    CDS135..2372
    Translation

    Target ORF information:

    RefSeq Version XM_010817940.3
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817940.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229200
    Clone ID Related Accession (Same CDS sequence) XM_010817941.3 , XM_010817939.3 , XM_010817940.3 , XM_010817938.3 , XM_024983104.1 , XM_024983105.1 , XM_024983106.1 , XM_010817937.3 , XM_010817935.3
    Accession Version XM_010817938.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817938.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816240.1
    CDS68..2305
    Translation

    Target ORF information:

    RefSeq Version XM_010817938.3
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817938.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229200
    Clone ID Related Accession (Same CDS sequence) XM_010817941.3 , XM_010817939.3 , XM_010817940.3 , XM_010817938.3 , XM_024983104.1 , XM_024983105.1 , XM_024983106.1 , XM_010817937.3 , XM_010817935.3
    Accession Version XM_024983104.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838872.1
    CDS34..2271
    Translation

    Target ORF information:

    RefSeq Version XM_024983104.1
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983104.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229200
    Clone ID Related Accession (Same CDS sequence) XM_010817941.3 , XM_010817939.3 , XM_010817940.3 , XM_010817938.3 , XM_024983104.1 , XM_024983105.1 , XM_024983106.1 , XM_010817937.3 , XM_010817935.3
    Accession Version XM_024983105.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838873.1
    CDS167..2404
    Translation

    Target ORF information:

    RefSeq Version XM_024983105.1
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983105.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229200
    Clone ID Related Accession (Same CDS sequence) XM_010817941.3 , XM_010817939.3 , XM_010817940.3 , XM_010817938.3 , XM_024983104.1 , XM_024983105.1 , XM_024983106.1 , XM_010817937.3 , XM_010817935.3
    Accession Version XM_024983106.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838874.1
    CDS102..2339
    Translation

    Target ORF information:

    RefSeq Version XM_024983106.1
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983106.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229200
    Clone ID Related Accession (Same CDS sequence) XM_010817941.3 , XM_010817939.3 , XM_010817940.3 , XM_010817938.3 , XM_024983104.1 , XM_024983105.1 , XM_024983106.1 , XM_010817937.3 , XM_010817935.3
    Accession Version XM_010817937.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817937.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816239.1
    CDS472..2709
    Translation

    Target ORF information:

    RefSeq Version XM_010817937.3
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817937.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229200
    Clone ID Related Accession (Same CDS sequence) XM_010817941.3 , XM_010817939.3 , XM_010817940.3 , XM_010817938.3 , XM_024983104.1 , XM_024983105.1 , XM_024983106.1 , XM_010817937.3 , XM_010817935.3
    Accession Version XM_010817935.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817935.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816237.1
    CDS88..2325
    Translation

    Target ORF information:

    RefSeq Version XM_010817935.3
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817935.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGCTGTGCTG GGTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229201
    Clone ID Related Accession (Same CDS sequence) XM_010817936.3
    Accession Version XM_010817936.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817936.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGG GGAGTTCTTT ACCGTCTGAG
    CTACCAGGGA ATCCCTCAAG ATGGATGAAT GAGATTAAAT CAACACATCC CCCCGACTCA
    GCCCTCTCCC ACAGTAACCT AGGTAAAGAA GCTCTTAGGA AAACGTGGCC TGTCATAGCA
    TTTCCTCAAG TAACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816238.1
    CDS88..2325
    Translation

    Target ORF information:

    RefSeq Version XM_010817936.3
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817936.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGG GGAGTTCTTT ACCGTCTGAG
    CTACCAGGGA ATCCCTCAAG ATGGATGAAT GAGATTAAAT CAACACATCC CCCCGACTCA
    GCCCTCTCCC ACAGTAACCT AGGTAAAGAA GCTCTTAGGA AAACGTGGCC TGTCATAGCA
    TTTCCTCAAG TAACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229202
    Clone ID Related Accession (Same CDS sequence) XM_010817942.3
    Accession Version XM_010817942.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2223bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817942.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA CACCAGCCGG GTGTCCTGCA
    GTTGAGTTCC ATTCTGACAC CCCGTCTGCC TGGAGGTTCC ATCAGATCCC ACAGCTGTGC
    TGGGTCTTAG CTCCCCGACC AGGGATGGAA CCCGGGCCCG CTGCATCAAG AGCGGAGTCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816244.2
    CDS88..2310
    Translation

    Target ORF information:

    RefSeq Version XM_010817942.3
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817942.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA CACCAGCCGG GTGTCCTGCA
    GTTGAGTTCC ATTCTGACAC CCCGTCTGCC TGGAGGTTCC ATCAGATCCC ACAGCTGTGC
    TGGGTCTTAG CTCCCCGACC AGGGATGGAA CCCGGGCCCG CTGCATCAAG AGCGGAGTCT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229203
    Clone ID Related Accession (Same CDS sequence) XM_024983107.1
    Accession Version XM_024983107.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2157bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGGTTCT CGATCAGTAT GAACGAGAAG GATTTAATTT CCTGGCGAAG
    GTGTTTAATT CTTCGCACTC CTTCTTAGAG GACTTGACCG GTCTTACATT GCTGCATCAA
    GAGACCCAGG CTGCTGAGAA GACTCTGGTA CTCCAAACCT GTAGGTTTCC CGCCCCCACA
    CCAAGCAGTT CTCTAATTCT CCACAGACAC CAGCCGGGTG TCCTGCAGTT GAGTTCCATT
    CTGACACCCC GTCTGCCTGG AGGTTCCATC AGATCCCACA GCTGTGCTGG GTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838875.1
    CDS89..2245
    Translation

    Target ORF information:

    RefSeq Version XM_024983107.1
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983107.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGGTTCT CGATCAGTAT GAACGAGAAG GATTTAATTT CCTGGCGAAG
    GTGTTTAATT CTTCGCACTC CTTCTTAGAG GACTTGACCG GTCTTACATT GCTGCATCAA
    GAGACCCAGG CTGCTGAGAA GACTCTGGTA CTCCAAACCT GTAGGTTTCC CGCCCCCACA
    CCAAGCAGTT CTCTAATTCT CCACAGACAC CAGCCGGGTG TCCTGCAGTT GAGTTCCATT
    CTGACACCCC GTCTGCCTGG AGGTTCCATC AGATCCCACA GCTGTGCTGG GTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229204
    Clone ID Related Accession (Same CDS sequence) XM_005223099.4
    Accession Version XM_005223099.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2154bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223099.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA ATAGAGCGAT TCTGGACACA
    GACTACCCTG TCCCTCCAGT GAAAAGGCAA GCACCGAATG GACAGTCGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223156.1
    CDS88..2241
    Translation

    Target ORF information:

    RefSeq Version XM_005223099.4
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223099.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA ATAGAGCGAT TCTGGACACA
    GACTACCCTG TCCCTCCAGT GAAAAGGCAA GCACCGAATG GACAGTCGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229205
    Clone ID Related Accession (Same CDS sequence) XM_024983108.1 , XM_024983110.1
    Accession Version XM_024983108.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA TCTGGGACAT GAGTGACGAC
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838876.1
    CDS465..2576
    Translation

    Target ORF information:

    RefSeq Version XM_024983108.1
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983108.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA TCTGGGACAT GAGTGACGAC
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229205
    Clone ID Related Accession (Same CDS sequence) XM_024983108.1 , XM_024983110.1
    Accession Version XM_024983110.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA TCTGGGACAT GAGTGACGAC
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838878.1
    CDS566..2677
    Translation

    Target ORF information:

    RefSeq Version XM_024983110.1
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983110.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA TCTGGGACAT GAGTGACGAC
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229206
    Clone ID Related Accession (Same CDS sequence) XM_010817945.3
    Accession Version XM_010817945.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2106bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817945.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA CGTACGACGA CCATAGGAAA
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816247.1
    CDS88..2193
    Translation

    Target ORF information:

    RefSeq Version XM_010817945.3
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817945.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA CGTACGACGA CCATAGGAAA
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229207
    Clone ID Related Accession (Same CDS sequence) XM_024983111.1
    Accession Version XM_024983111.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGG TGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838879.1
    CDS88..2175
    Translation

    Target ORF information:

    RefSeq Version XM_024983111.1
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983111.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGG TGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229208
    Clone ID Related Accession (Same CDS sequence) XM_024983112.1
    Accession Version XM_024983112.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2100bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGG TGTATAAACA AGAGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838880.1
    CDS469..2568
    Translation

    Target ORF information:

    RefSeq Version XM_024983112.1
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983112.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGG TGTATAAACA AGAGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229209
    Clone ID Related Accession (Same CDS sequence) XM_024983113.1
    Accession Version XM_024983113.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1989bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAATCT GGGACATGAG TGACGACGAG
    ACCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838881.1
    CDS88..2076
    Translation

    Target ORF information:

    RefSeq Version XM_024983113.1
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983113.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAATCT GGGACATGAG TGACGACGAG
    ACCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229210
    Clone ID Related Accession (Same CDS sequence) XM_024983114.1
    Accession Version XM_024983114.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGA
    TACGGGGATT TCTGTCACGG TTTGATAACG TCCTTCCTGT CAGCCTGGCC TTTGACAAAT
    GCACGGCTTG TTCTTCCAAA ATCTGGGACA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838882.1
    CDS88..1980
    Translation

    Target ORF information:

    RefSeq Version XM_024983114.1
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983114.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTCG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGA
    TACGGGGATT TCTGTCACGG TTTGATAACG TCCTTCCTGT CAGCCTGGCC TTTGACAAAT
    GCACGGCTTG TTCTTCCAAA ATCTGGGACA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94472
    Clone ID Related Accession (Same CDS sequence) XM_015459680.1
    Accession Version XM_015459680.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104545.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315166.1
    CDS90..2315
    Translation

    Target ORF information:

    RefSeq Version XM_015459680.1
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459680.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAC CGCATGAATG AGCCGCCAAC GTCCCTCGGC
    CTCGTGCCTC ACCAGATACG GGGATTTCTG TCACGGTTTG ATAACGTCCT TCCTGTCAGC
    CTGGCCTTTG ACAAATGCAC GGCTTGTTCT TCCAAAGTTC TCGATCAGTA TGAACGAGAA
    GGATTTAATT TCCTGGCGAA GGTGTTTAAT TCTTCGCACT CCTTCTTAGA GGACTTGACC
    GGTCTTACAT TGCTGCATCA AGAGACCCAG GCTGCTGAGA AGACTCTGGT ACTCCAAACC
    TGTAGGTTTC CCGCCCCCAC ACCAAGCAGT TCTCTAATTC TCCACAGACA CCAGCCGGGT
    GTCCTGCAGT TGAGTTCCAT TCTGACACCC CGTCTGCCTG GAGGTTCCAT CAGATCCCAC
    AGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94473
    Clone ID Related Accession (Same CDS sequence) XM_015459681.1
    Accession Version XM_015459681.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104545.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGA
    TACGGGGATT TCTGTCACGG TTTGATAACG TCCTTCCTGT CAGCCTGGCC TTTGACAAAT
    GCACGGCTTG TTCTTCCAAA GTTCTCGATC AGTATGAACG AGAAGGATTT AATTTCCTGG
    CGAAGGTGTT TAATTCTTCG CACTCCTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315167.1
    CDS90..2042
    Translation

    Target ORF information:

    RefSeq Version XM_015459681.1
    Organism Bos taurus(cattle)
    Definition Bos taurus autophagy related 7 (ATG7), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459681.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCAGCAG CTATGGGGGA CCTCGGCCTC TCCAGATTGC AGTTCGCCCC CTTCAGCAGC 
    GCCTTGGATG TTGGCTTCTG GCATGAGTTG ACCCAGAAGA AGCTGAATGA GTACCGGCTA
    GACGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAACG GTGACTCTGT GGGGCTGCCA
    GCTCGCTTAA CGTTGGAGTT CAGTGCTTTC GACATGAGTG CACCCACCCC AGCCCGCTGC
    TGCCCGGCGG TCGGCATACT GTATAATACC AACACACTCG AGGCTTTCAA GGCTGCAGAT
    AAGAAGCTAC TTCTGGAAGA AGCGGCAAAC GAGATCTGGG AGTCCATCAA ATCGGGGGCC
    GCCCTGGACA ACCCCGTGCT CCTCAACAAG TTCCTCCTCT TGACATTTGC GGATCTAAAG
    AAGTACCATT TCTACTACTG GTTCTGCTCC CCAGCTCTCT GCCTTCCAGA GAGCATACCC
    CTCATTCAGG GGCCAGTGGC CTTGGATCAA TGGTTTTTGC CAAAACAGAT TCAAGCCCTC
    GAGCACGCGT ATGATGCCCT TTGTCAGACA GAAGGAGTCC CAGCACTGCC TTACTTCCTA
    ATCAAGTATG ACGAGACCAC GGTGCTGGTT TCCTCGCTCA AACACTACAG CGATTTCTTC
    CAGGGTCAAA GGACAAAGAT AACTATTGGC GTGTACGATC CCTGTAACTT AGCACAGTAC
    CCCGGATGGC CTTTGAGGAA CCTTTTGGTC CTAGCAGCCC ACAGATGGAG TGGCAGTTTC
    CAGTCCGTGG AAGTGGTCTG CTTCCGGGAC CGCACCCTGC AGGGGGTGAG AGACGTCACC
    CACAGCATCG TCTTCGAAGT GAAGCTTCCA GAGATGGCGT TCAGCCCAGA TTGTCCCAAG
    GCGGTCGGAT GGGAAAAGAA CCAGAAGGGA GGCATGGGAC CGAGGATGGT GAACCTCAGC
    GAGTGTATGG ACCCTAAAAG GTTAGCTGAG TCATCGGTTG ATCTCAACCT CAAGCTGATG
    TGTTGGAGAT TGGTCCCTAC TCTGGACTTA GACAAGGTTG TGTCTGCCAA GTGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGTAACGTA GCCCGGACGT TGATGGGTTG GGGTGTCAGA
    CACATCACGT TCGTGGACAA TGCCAAGATC TCCTACTCTA ACCCTGTGAG GCAGCCTCTG
    TATGAATTTG AAGATTGCCT GGCGGGTGGG AAGCCCAAGG CCCTGGCAGC CGCAGACCGG
    CTGCAGAAGA TATTCCCCGG AGTGAACGCC AGAGGGTTCA ACATGAGCAT CCCCATGCCG
    GGGCACCCCG TGAACTTCTC CAGCGTCACC CTGGAGCAGG CCCGCAGGGA CGTGGAACAG
    CTGGAGCAGC TCATTGACAG CCACGACGTC GTCTTCCTGT TGATGGACAC CCGGGAGAGC
    CGGTGGCTGC CTGCCGTCAT CGCCGCGAGC AAGAGAAAGC TGGTCATCAA TGCTGCCTTG
    GGATTTGACA CCTTCGTTGT CATGAGACAC GGCCTGAAGA AGCCCAGGCA TCAGGGAGCT
    GGGGACTTGT GTCCCAGCTA CCCCATGGCG TCTGCCGACC TCCTGAGCTC ATCGCTGTTT
    GCCAACATCC CTGGCTATAA ACTCGGCTGC TACTTCTGCA ACGATGTGGT GGCCCCAGGG
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGCCGTCC AGGACTAGCC
    ATGATCGCAG GAGCCCTGGC GGTGGAGTTG ATGGTTTCCG TTTTGCAGCA TCCAGAAGGA
    TACGGGGATT TCTGTCACGG TTTGATAACG TCCTTCCTGT CAGCCTGGCC TTTGACAAAT
    GCACGGCTTG TTCTTCCAAA GTTCTCGATC AGTATGAACG AGAAGGATTT AATTTCCTGG
    CGAAGGTGTT TAATTCTTCG CACTCCTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.