CFAP54 cDNA ORF clone, Bos taurus(cattle)

The following CFAP54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CFAP54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa234806 XM_024992514.1
Latest version!
Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa89908 XM_015463365.1
Latest version!
Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa89908 XM_015471152.1
Latest version!
Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa234798 XM_024992506.1
Latest version!
Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa234799 XM_024992507.1
Latest version!
Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa234800 XM_024992508.1
Latest version!
Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa234801 XM_024992509.1
Latest version!
Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa234802 XM_024992510.1
Latest version!
Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa234803 XM_024992511.1
Latest version!
Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa234804 XM_024992512.1
Latest version!
Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa234805 XM_024992513.1
Latest version!
Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89899 XM_015463358.1
Latest version!
Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89899 XM_015471145.1
Latest version!
Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89900 XM_005198332.3
Latest version!
Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89900 XM_002687523.6
Latest version!
Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89901 XM_015471146.1
Latest version!
Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89901 XM_015463359.1
Latest version!
Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89902 XM_015471147.1
Latest version!
Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89902 XM_015463360.1
Latest version!
Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89903 XM_015463361.1
Latest version!
Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89903 XM_015471148.1
Latest version!
Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89904 XM_015471149.1
Latest version!
Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89904 XM_015463362.1
Latest version!
Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89905 XM_015463363.1
Latest version!
Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89905 XM_015471150.1
Latest version!
Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89906 XM_010805261.3
Latest version!
Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89906 XM_010827774.2
Latest version!
Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89907 XM_015463364.1
Latest version!
Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89907 XM_015471151.1
Latest version!
Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OBa89900 XM_002687523.5 Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89906 XM_010805261.2 Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa234806
    Clone ID Related Accession (Same CDS sequence) XM_024992514.1
    Accession Version XM_024992514.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7728bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA 
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GGAGACAAAG GAGGAGTGAA CAATTTAAAA
    TTTGCTACTG GGAAGGTGGA GTTTTCCATG GGGACAGAAG AGATGCACAT GCCAACACCC
    CCCGACCTTT CTCAGGAGCA CTTCCGAGTT TTCAGCTCAG TGGAGAAGAG CAAGCTTCCC
    TCCTCACAAC TTGGACTCGT AATTTCTTCT TATTATGAAA CTATTAATGT TCTTCAAACC
    AGCAATCAAA GAAGTCTTCA TGTGCAGGCG TTGCATGAAC TTGGAAATCT TCTCATCTTC
    GCACAGAAGA AAAGGGCTGC TTTTAAGTGT TGGAGTCAAG CTCTGGATGA AATTTTCAGA
    AAAGAAGATG TGCTCCATAC ATGGAAGGAG ATTGGCAGCT CACTCACCAG TGCCACTGAT
    GGCTGGTCAC CTCCAGGTTC CAAAGACTAC AGTGAGGAGC TCCTGTCCAA AGGCGGCATC
    TGGGGGTGTT TGCAGGGAGC GGTGATATCA GCAAAGATAG CACAATTTAT TCAGACATTG
    GATGTTGAAA AGAGAACCAA TTGTTGCCTT TTATCAGCAT TACTCTTTCA GGGTTTGCTT
    AGAACCACAC TGCCGCATCC TAAAGCTGAA CGTTGCTACG CTCAGTATGA AATCACTCAG
    CTTCTCCCGG GCATCGAGCT CTTCTCAGAC AGATATCGGG CTGACATCTG CTCCGTCATT
    GCGAGTTTAT ATTACGTCAT CCGCGAGCTG CACTTTGCTA AGCAAAATCT AGTAATTCTG
    CCTCTCCTTG CATTGTACCA ATATTTTGTT TCTGTAATTT GCCAAGACCT AGTCAAAAAT
    CTGGAAGCAA GAATCCTCAA GATAGAAGTC CTTACAGATT TAGGATTCTT TTCCGAAGCC
    TTTCATGAGC TATCCCAGAT ATTTTATGCG AAAAACACAC CTTCTTCAGT ACCCACCAGC
    TGTAAACCTA CTGGAAAAAT GAAGCTATTT CAATCATTTG ACTCGGGAAA ACCTCTTATT
    AGTAAAGAGA ATCTGCAGGC ACTTGATGAT TTAATAAATA AAGGAATGCC TGTAATTCTG
    ACTACAGTCA GCCAGCAACA TCTCTTAAAT AAGTTTTTAG TTGTTAAAGC ATACTTTTTC
    ATAAATGTGG CTGCAACAAT AAATTGTGTC CCAGAACAGA CAATGAAACC AGTCGGCCAT
    GGAGTTATGA ATGAGAAAAG CAAACCAAAT CTTCCAAACT TGAAAGAGAT GTGTTTAAAG
    GATGAGGGAG GCTCACTTTG TCATCTGACA AAATTGAAAG AGAACTTTGC TCTTAGCACA
    CTAAAGTCAA TTTTACTGAC AGAAGCTGAA GACAGACTCA ACACCCTTCT GTCGGAGATG
    GACCATCTAA GCCATAAGGA CCTCTCTCAG TGTTCTGCTG GCGAACTGGA GATTGTAGTG
    GAGGTCAGAC TGCAGCTGGC TGCCATTGCC CTGCAGAGAC ACCGGGCAGC ATACAGTGCT
    GCGATAGTAT TTTCCACACT TAAACTTCTC CAGGATTCAA AACTTTTTAA AAAGAAAGTA
    GTAGAAGATG ACTCAGAGAA TTGCACCTCT TCAGAAAGTT ATGCGACTGA AAGTAAAGAC
    GACAATTTTT TAGATCCTAT TTCCTTAAAT TCTCGAGAAT ATTTCAACAT TCATCTGTGG
    TTGAGGTGCC GCTTAGCCTT GGTGACTGCA TTTGTTGCCC AGATTCGTGG CATTGGAATC
    GTGAAAGAAA ACGATATAAC AGATTACGTG AGCCTTATCA ATGAAGTGTG TGTGGAGGCA
    AAAGCTGCAG GTGACAGGGA GCTACAGGCT GAATTCTTGA TGCAAGCTGT AATTATCGGC
    CTACAAGAAA AGCATTTAAA GGCAGACATC ATAAAAAGCC TTCAGGAGGT AATACAGTTG
    CTTGAAGGGC ATGACTTTAT TTCTCCTCGA TCTCATTTAA CCCTGGTGAG AAGCATGCTT
    TTGCTGGATG ATTTAACAAA AGCTGAGAAA TTCAAGGAAA CTCCTTTTTC GAAAATAGAA
    AAATTGTATT TGTTGACTCA GTCTCACAAC ATTCTTATAG AACAGATGAT AACTTTTGGA
    GAAACAATTG AATTGCCTTC ATCAAACACT GACTATGCAA GTCCATTACT GCCTTTGAAA
    AATATTTATC TTCCTCACGT CATGTTGTTG GCCAAAACAA AAATGAGAAT TGGACATACA
    GTGGCCAAGC AAGTATATTA TACCAGTAAA AAGAAGGATT CTTCAAAGTG GTTACCTGCC
    CTTCATTTTT TTGAAATAGC TTTGAAGCTC GCTAGGACAT CAGGAACAGA AGAACATGAG
    GTGGAAGCTG AAATCTTTTT TCAGAAAGGC AAAATAGAAT GTCAAATATT GATGGAAGAG
    AAACCTCCAA CTATGCAACT TGAGAGTCTG TTTGAAGCTA TACAGCTAAG CTTGAGAAAT
    GATCAAAATT CAGGGTTGAT AAGAGACTCC TACCTTGAAA TAGCCTTATT ATTTTTACAC
    ATGAGGAAGT TAAAGAGCAA ACTTTCAGTG TCACCATTGA TGAGTATTAA GGCTCTTCCC
    AGAAGGCATA GTTCTATTAA AGAACCAGTA GTAAATAAAC ATGAAATGTA CAGTTTATTT
    GCCTGGATTG CAATAAGAGC TGCTGCACAG GTCAGTGAGG CTGTGCTGGC AATTAATCTA
    CTTATTGGAA AGAAGAATGC TAGAACTGAT ACAGTTAGCC AAGGGGCATT ATCAAATATT
    CCAGAATTTG CTTCCATGGA TCTTTTGTCT TCATACACAG ATTATTTGCT TGATAACTAC
    CAAGTTGTCT TCCAGACTGG TTGTACATTT TCTTACGTTA GTGAAGAGAT ACATGAAGAT
    GTAGATTCTC AGAAAAAAAA CCCAATTAAA GTGGATATTA CATGGATTCT TCTGCTGCGC
    TACTATATTC ACCTACAAAG AATTAATAAT ATGAGCAAAT TGCTAGCATC TCTGAAGCCA
    GGATCTGGGA TGTCTTTGCC AGATGACACT CTTCTCACTT CTCTTTTTAA CTCTGGATTG
    ATTTTGCGCC AAAAAGAAAT GCATTTTTTC CTTAAAAGAT TCTTACAGCT ATATTCTTCT
    TCTTGTATTG ATGAATTTCC AAAAGAACTA TTTCAAGTCA TGGAACATCC ACCTGTTCTA
    GAAAAAGTCT TATATGATTC AGCAAGCAAG ACAGGAGTAA CCAATGCTTC TCTATGCTCC
    GATTTATCAC TAAAGACCGT TAGCAGCACA TTGTATGGAG ACGTGGTGCC ACCAGTTGTG
    GCAACACAGG CTTTAAACAA AGAACTTTGT TTTCAGTGGT ACATTCCTCC CCTGGAAAAA
    CCTCCAAAGG AAACAGAACC TATGGTTTTA TTGTTGTATG CATATAATAT GAAGCCCCTG
    AATATTTCTG ATGTTGCATC TGCTGCTTGC AACTGTGTGT ACGTAGGCTC TTTCTGGGTT
    CCACTAAAAA GAGTTATTTC AGTTCATGAG AAACTATCTA ATCTTACGCA AATTGCTGAA
    CTATCATTGC CAACAACTCC TAAAGTTACT TCAAATGAAA ATTTACATGA AGTAGAGGAA
    ACAGAAGAGA AATCAATTGA TGCAGAAATG GAAAACATGA TTATTGTCTG TTGCAATGAA
    ATAGTATCTT TGTTTTTGGG TGATGAAATG GCAACTCCAC TATCTGAGGT CCCATTTGAT
    GTCTCGCTGC CATCTATATT CAGTCTGGAG AGACTTTTTG ATCTTGCTAG TGGCTGTATC
    GTATCGGGAG GAAGCCTTTT CAACTGGGTG GTATCAATTA TTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848282.1
    CDS137..7864
    Translation

    Target ORF information:

    RefSeq Version XM_024992514.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992514.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA 
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GGAGACAAAG GAGGAGTGAA CAATTTAAAA
    TTTGCTACTG GGAAGGTGGA GTTTTCCATG GGGACAGAAG AGATGCACAT GCCAACACCC
    CCCGACCTTT CTCAGGAGCA CTTCCGAGTT TTCAGCTCAG TGGAGAAGAG CAAGCTTCCC
    TCCTCACAAC TTGGACTCGT AATTTCTTCT TATTATGAAA CTATTAATGT TCTTCAAACC
    AGCAATCAAA GAAGTCTTCA TGTGCAGGCG TTGCATGAAC TTGGAAATCT TCTCATCTTC
    GCACAGAAGA AAAGGGCTGC TTTTAAGTGT TGGAGTCAAG CTCTGGATGA AATTTTCAGA
    AAAGAAGATG TGCTCCATAC ATGGAAGGAG ATTGGCAGCT CACTCACCAG TGCCACTGAT
    GGCTGGTCAC CTCCAGGTTC CAAAGACTAC AGTGAGGAGC TCCTGTCCAA AGGCGGCATC
    TGGGGGTGTT TGCAGGGAGC GGTGATATCA GCAAAGATAG CACAATTTAT TCAGACATTG
    GATGTTGAAA AGAGAACCAA TTGTTGCCTT TTATCAGCAT TACTCTTTCA GGGTTTGCTT
    AGAACCACAC TGCCGCATCC TAAAGCTGAA CGTTGCTACG CTCAGTATGA AATCACTCAG
    CTTCTCCCGG GCATCGAGCT CTTCTCAGAC AGATATCGGG CTGACATCTG CTCCGTCATT
    GCGAGTTTAT ATTACGTCAT CCGCGAGCTG CACTTTGCTA AGCAAAATCT AGTAATTCTG
    CCTCTCCTTG CATTGTACCA ATATTTTGTT TCTGTAATTT GCCAAGACCT AGTCAAAAAT
    CTGGAAGCAA GAATCCTCAA GATAGAAGTC CTTACAGATT TAGGATTCTT TTCCGAAGCC
    TTTCATGAGC TATCCCAGAT ATTTTATGCG AAAAACACAC CTTCTTCAGT ACCCACCAGC
    TGTAAACCTA CTGGAAAAAT GAAGCTATTT CAATCATTTG ACTCGGGAAA ACCTCTTATT
    AGTAAAGAGA ATCTGCAGGC ACTTGATGAT TTAATAAATA AAGGAATGCC TGTAATTCTG
    ACTACAGTCA GCCAGCAACA TCTCTTAAAT AAGTTTTTAG TTGTTAAAGC ATACTTTTTC
    ATAAATGTGG CTGCAACAAT AAATTGTGTC CCAGAACAGA CAATGAAACC AGTCGGCCAT
    GGAGTTATGA ATGAGAAAAG CAAACCAAAT CTTCCAAACT TGAAAGAGAT GTGTTTAAAG
    GATGAGGGAG GCTCACTTTG TCATCTGACA AAATTGAAAG AGAACTTTGC TCTTAGCACA
    CTAAAGTCAA TTTTACTGAC AGAAGCTGAA GACAGACTCA ACACCCTTCT GTCGGAGATG
    GACCATCTAA GCCATAAGGA CCTCTCTCAG TGTTCTGCTG GCGAACTGGA GATTGTAGTG
    GAGGTCAGAC TGCAGCTGGC TGCCATTGCC CTGCAGAGAC ACCGGGCAGC ATACAGTGCT
    GCGATAGTAT TTTCCACACT TAAACTTCTC CAGGATTCAA AACTTTTTAA AAAGAAAGTA
    GTAGAAGATG ACTCAGAGAA TTGCACCTCT TCAGAAAGTT ATGCGACTGA AAGTAAAGAC
    GACAATTTTT TAGATCCTAT TTCCTTAAAT TCTCGAGAAT ATTTCAACAT TCATCTGTGG
    TTGAGGTGCC GCTTAGCCTT GGTGACTGCA TTTGTTGCCC AGATTCGTGG CATTGGAATC
    GTGAAAGAAA ACGATATAAC AGATTACGTG AGCCTTATCA ATGAAGTGTG TGTGGAGGCA
    AAAGCTGCAG GTGACAGGGA GCTACAGGCT GAATTCTTGA TGCAAGCTGT AATTATCGGC
    CTACAAGAAA AGCATTTAAA GGCAGACATC ATAAAAAGCC TTCAGGAGGT AATACAGTTG
    CTTGAAGGGC ATGACTTTAT TTCTCCTCGA TCTCATTTAA CCCTGGTGAG AAGCATGCTT
    TTGCTGGATG ATTTAACAAA AGCTGAGAAA TTCAAGGAAA CTCCTTTTTC GAAAATAGAA
    AAATTGTATT TGTTGACTCA GTCTCACAAC ATTCTTATAG AACAGATGAT AACTTTTGGA
    GAAACAATTG AATTGCCTTC ATCAAACACT GACTATGCAA GTCCATTACT GCCTTTGAAA
    AATATTTATC TTCCTCACGT CATGTTGTTG GCCAAAACAA AAATGAGAAT TGGACATACA
    GTGGCCAAGC AAGTATATTA TACCAGTAAA AAGAAGGATT CTTCAAAGTG GTTACCTGCC
    CTTCATTTTT TTGAAATAGC TTTGAAGCTC GCTAGGACAT CAGGAACAGA AGAACATGAG
    GTGGAAGCTG AAATCTTTTT TCAGAAAGGC AAAATAGAAT GTCAAATATT GATGGAAGAG
    AAACCTCCAA CTATGCAACT TGAGAGTCTG TTTGAAGCTA TACAGCTAAG CTTGAGAAAT
    GATCAAAATT CAGGGTTGAT AAGAGACTCC TACCTTGAAA TAGCCTTATT ATTTTTACAC
    ATGAGGAAGT TAAAGAGCAA ACTTTCAGTG TCACCATTGA TGAGTATTAA GGCTCTTCCC
    AGAAGGCATA GTTCTATTAA AGAACCAGTA GTAAATAAAC ATGAAATGTA CAGTTTATTT
    GCCTGGATTG CAATAAGAGC TGCTGCACAG GTCAGTGAGG CTGTGCTGGC AATTAATCTA
    CTTATTGGAA AGAAGAATGC TAGAACTGAT ACAGTTAGCC AAGGGGCATT ATCAAATATT
    CCAGAATTTG CTTCCATGGA TCTTTTGTCT TCATACACAG ATTATTTGCT TGATAACTAC
    CAAGTTGTCT TCCAGACTGG TTGTACATTT TCTTACGTTA GTGAAGAGAT ACATGAAGAT
    GTAGATTCTC AGAAAAAAAA CCCAATTAAA GTGGATATTA CATGGATTCT TCTGCTGCGC
    TACTATATTC ACCTACAAAG AATTAATAAT ATGAGCAAAT TGCTAGCATC TCTGAAGCCA
    GGATCTGGGA TGTCTTTGCC AGATGACACT CTTCTCACTT CTCTTTTTAA CTCTGGATTG
    ATTTTGCGCC AAAAAGAAAT GCATTTTTTC CTTAAAAGAT TCTTACAGCT ATATTCTTCT
    TCTTGTATTG ATGAATTTCC AAAAGAACTA TTTCAAGTCA TGGAACATCC ACCTGTTCTA
    GAAAAAGTCT TATATGATTC AGCAAGCAAG ACAGGAGTAA CCAATGCTTC TCTATGCTCC
    GATTTATCAC TAAAGACCGT TAGCAGCACA TTGTATGGAG ACGTGGTGCC ACCAGTTGTG
    GCAACACAGG CTTTAAACAA AGAACTTTGT TTTCAGTGGT ACATTCCTCC CCTGGAAAAA
    CCTCCAAAGG AAACAGAACC TATGGTTTTA TTGTTGTATG CATATAATAT GAAGCCCCTG
    AATATTTCTG ATGTTGCATC TGCTGCTTGC AACTGTGTGT ACGTAGGCTC TTTCTGGGTT
    CCACTAAAAA GAGTTATTTC AGTTCATGAG AAACTATCTA ATCTTACGCA AATTGCTGAA
    CTATCATTGC CAACAACTCC TAAAGTTACT TCAAATGAAA ATTTACATGA AGTAGAGGAA
    ACAGAAGAGA AATCAATTGA TGCAGAAATG GAAAACATGA TTATTGTCTG TTGCAATGAA
    ATAGTATCTT TGTTTTTGGG TGATGAAATG GCAACTCCAC TATCTGAGGT CCCATTTGAT
    GTCTCGCTGC CATCTATATT CAGTCTGGAG AGACTTTTTG ATCTTGCTAG TGGCTGTATC
    GTATCGGGAG GAAGCCTTTT CAACTGGGTG GTATCAATTA TTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89908
    Clone ID Related Accession (Same CDS sequence) XM_015471152.1 , XM_015463365.1
    Accession Version XM_015471152.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA 
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGACAGGAG TAACCAATGC TTCTCTATGC
    TCCGATTTAT CACTAAAGAC CGTTAGCAGC ACATTGTATG GAGACGTGGT GCCACCAGTT
    GTGGCAACAC AGGCTTTAAA CAAAGAACTT TGTTTTCAGT GGTACATTCC TCCCCTGGAA
    AAACCTCCAA AGGAAACAGA ACCTATGGTT TTATTGTTGT ATGCATATAA TATGAAGCCC
    CTGAATATTT CTGATGTTGC ATCTGCTGCT TGCAACTGTG TGTACGTAGG CTCTTTCTGG
    GTTCCACTAA AAAGAGTTAT TTCAGTTCAT GAGAAACTAT CTAATCTTAC GCAAATTGCT
    GAACTATCAT TGCCAACAAC TCCTAAAGTT ACTTCAAATG AAAATTTACA TGAAGTAGAG
    GAAACAGAAG AGAAATCAAT TGATGCAGAA ATGGAAAACA TGATTATTGT CTGTTGCAAT
    GAAATAGTAT CTTTGTTTTT GGGTGATGAA ATGGCAACTC CACTATCTGA GGTCCCATTT
    GATGTCTCGC TGCCATCTAT ATTCAGTCTG GAGAGACTTT TTGATCTTGC TAGTGGCTGT
    ATCGTATCGG GAGGAAGCCT TTTCAACTGG GTGGTATCAA TTATTCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326638.1
    CDS91..7821
    Translation

    Target ORF information:

    RefSeq Version XM_015471152.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471152.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA 
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGACAGGAG TAACCAATGC TTCTCTATGC
    TCCGATTTAT CACTAAAGAC CGTTAGCAGC ACATTGTATG GAGACGTGGT GCCACCAGTT
    GTGGCAACAC AGGCTTTAAA CAAAGAACTT TGTTTTCAGT GGTACATTCC TCCCCTGGAA
    AAACCTCCAA AGGAAACAGA ACCTATGGTT TTATTGTTGT ATGCATATAA TATGAAGCCC
    CTGAATATTT CTGATGTTGC ATCTGCTGCT TGCAACTGTG TGTACGTAGG CTCTTTCTGG
    GTTCCACTAA AAAGAGTTAT TTCAGTTCAT GAGAAACTAT CTAATCTTAC GCAAATTGCT
    GAACTATCAT TGCCAACAAC TCCTAAAGTT ACTTCAAATG AAAATTTACA TGAAGTAGAG
    GAAACAGAAG AGAAATCAAT TGATGCAGAA ATGGAAAACA TGATTATTGT CTGTTGCAAT
    GAAATAGTAT CTTTGTTTTT GGGTGATGAA ATGGCAACTC CACTATCTGA GGTCCCATTT
    GATGTCTCGC TGCCATCTAT ATTCAGTCTG GAGAGACTTT TTGATCTTGC TAGTGGCTGT
    ATCGTATCGG GAGGAAGCCT TTTCAACTGG GTGGTATCAA TTATTCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89908
    Clone ID Related Accession (Same CDS sequence) XM_015471152.1 , XM_015463365.1
    Accession Version XM_015463365.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644803.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA 
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGACAGGAG TAACCAATGC TTCTCTATGC
    TCCGATTTAT CACTAAAGAC CGTTAGCAGC ACATTGTATG GAGACGTGGT GCCACCAGTT
    GTGGCAACAC AGGCTTTAAA CAAAGAACTT TGTTTTCAGT GGTACATTCC TCCCCTGGAA
    AAACCTCCAA AGGAAACAGA ACCTATGGTT TTATTGTTGT ATGCATATAA TATGAAGCCC
    CTGAATATTT CTGATGTTGC ATCTGCTGCT TGCAACTGTG TGTACGTAGG CTCTTTCTGG
    GTTCCACTAA AAAGAGTTAT TTCAGTTCAT GAGAAACTAT CTAATCTTAC GCAAATTGCT
    GAACTATCAT TGCCAACAAC TCCTAAAGTT ACTTCAAATG AAAATTTACA TGAAGTAGAG
    GAAACAGAAG AGAAATCAAT TGATGCAGAA ATGGAAAACA TGATTATTGT CTGTTGCAAT
    GAAATAGTAT CTTTGTTTTT GGGTGATGAA ATGGCAACTC CACTATCTGA GGTCCCATTT
    GATGTCTCGC TGCCATCTAT ATTCAGTCTG GAGAGACTTT TTGATCTTGC TAGTGGCTGT
    ATCGTATCGG GAGGAAGCCT TTTCAACTGG GTGGTATCAA TTATTCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318851.1
    CDS91..7821
    Translation

    Target ORF information:

    RefSeq Version XM_015463365.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463365.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA 
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGACAGGAG TAACCAATGC TTCTCTATGC
    TCCGATTTAT CACTAAAGAC CGTTAGCAGC ACATTGTATG GAGACGTGGT GCCACCAGTT
    GTGGCAACAC AGGCTTTAAA CAAAGAACTT TGTTTTCAGT GGTACATTCC TCCCCTGGAA
    AAACCTCCAA AGGAAACAGA ACCTATGGTT TTATTGTTGT ATGCATATAA TATGAAGCCC
    CTGAATATTT CTGATGTTGC ATCTGCTGCT TGCAACTGTG TGTACGTAGG CTCTTTCTGG
    GTTCCACTAA AAAGAGTTAT TTCAGTTCAT GAGAAACTAT CTAATCTTAC GCAAATTGCT
    GAACTATCAT TGCCAACAAC TCCTAAAGTT ACTTCAAATG AAAATTTACA TGAAGTAGAG
    GAAACAGAAG AGAAATCAAT TGATGCAGAA ATGGAAAACA TGATTATTGT CTGTTGCAAT
    GAAATAGTAT CTTTGTTTTT GGGTGATGAA ATGGCAACTC CACTATCTGA GGTCCCATTT
    GATGTCTCGC TGCCATCTAT ATTCAGTCTG GAGAGACTTT TTGATCTTGC TAGTGGCTGT
    ATCGTATCGG GAGGAAGCCT TTTCAACTGG GTGGTATCAA TTATTCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234798
    Clone ID Related Accession (Same CDS sequence) XM_024992506.1
    Accession Version XM_024992506.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GGAGACAAAG GAGGAGTGAA CAATTTAAAA
    TTTGCTACTG GGAAGGTGGA GTTTTCCATG GGGACAGAAG AGATGCACAT GCCAACACCC
    CCCGACCTTT CTCAGGAGCA CTTCCGAGTT TTCAGCTCAG TGGAGAAGAG CAAGCTTCCC
    TCCTCACAAC TTGGACTCGT AATTTCTTCT TATTATGAAA CTATTAATGT TCTTCAAACC
    AGCAATCAAA GAAGTCTTCA TGTGCAGGCG TTGCATGAAC TTGGAAATCT TCTCATCTTC
    GCACAGAAGA AAAGGGCTGC TTTTAAGTGT TGGAGTCAAG CTCTGGATGA AATTTTCAGA
    AAAGAAGATG TGCTCCATAC ATGGAAGGAG ATTGGCAGCT CACTCACCAG TGCCACTGAT
    GGCTGGTCAC CTCCAGGTTC CAAAGACTAC AGTGAGGAGC TCCTGTCCAA AGGCGGCATC
    TGGGGGTGTT TGCAGGGAGC GGTGATATCA GCAAAGATAG CACAATTTAT TCAGACATTG
    GATGTTGAAA AGAGAACCAA TTGTTGCCTT TTATCAGCAT TACTCTTTCA GGGTTTGCTT
    AGAACCACAC TGCCGCATCC TAAAGCTGAA CGTTGCTACG CTCAGTATGA AATCACTCAG
    CTTCTCCCGG GCATCGAGCT CTTCTCAGAC AGATATCGGG CTGACATCTG CTCCGTCATT
    GCGAGTTTAT ATTACGTCAT CCGCGAGCTG CACTTTGCTA AGCAAAATCT AGTAATTCTG
    CCTCTCCTTG CATTGTACCA ATATTTTGTT TCTGTAATTT GCCAAGACCT AGTCAAAAAT
    CTGGAAGCAA GAATCCTCAA GATAGAAGTC CTTACAGATT TAGGATTCTT TTCCGAAGCC
    TTTCATGAGC TATCCCAGAT ATTTTATGCG AAAAACACAC CTTCTTCAGT ACCCACCAGC
    TGTAAACCTA CTGGAAAAAT GAAGGCACTT GATGATTTAA TAAATAAAGG AATGCCTGTA
    ATTCTGACTA CAGTCAGCCA GCAACATCTC TTAAATAAGT TTTTAGTTGT TAAAGCATAC
    TTTTTCATAA ATGTGGCTGC AACAATAAAT TGTGTCCCAG AACAGACAAT GAAACCAGTC
    GGCCATGGAG TTATGAATGA GAAAAGCAAA CCAAATCTTC CAAACTTGAA AGAGATGTGT
    TTAAAGGATG AGGGAGGCTC ACTTTGTCAT CTGACAAAAT TGAAAGAGAA CTTTGCTCTT
    AGCACACTAA AGTCAATTTT ACTGACAGAA GCTGAAGACA GACTCAACAC CCTTCTGTCG
    GAGATGGACC ATCTAAGCCA TAAGGACCTC TCTCAGTGTT CTGCTGGCGA ACTGGAGATT
    GTAGTGGAGG TCAGACTGCA GCTGGCTGCC ATTGCCCTGC AGAGACACCG GGCAGCATAC
    AGTGCTGCGA TAGTATTTTC CACACTTAAA CTTCTCCAGG ATTCAAAACT TTTTAAAAAG
    AAAGTAGTAG AAGATGACTC AGAGAATTGC ACCTCTTCAG AAAGTTATGC GACTGAAAGT
    AAAGACGACA ATTTTTTAGA TCCTATTTCC TTAAATTCTC GAGAATATTT CAACATTCAT
    CTGTGGTTGA GGTGCCGCTT AGCCTTGGTG ACTGCATTTG TTGCCCAGAT TCGTGGCATT
    GGAATCGTGA AAGAAAACGA TATAACAGAT TACGTGAGCC TTATCAATGA AGTGTGTGTG
    GAGGCAAAAG CTGCAGGTGA CAGGGAGCTA CAGGCTGAAT TCTTGATGCA AGCTGTAATT
    ATCGGCCTAC AAGAAAAGCA TTTAAAGGCA GACATCATAA AAAGCCTTCA GGAGGTAATA
    CAGTTGCTTG AAGGGCATGA CTTTATTTCT CCTCGATCTC ATTTAACCCT GGTGAGAAGC
    ATGCTTTTGC TGGATGATTT AACAAAAGCT GAGAAATTCA AGGAAACTCC TTTTTCGAAA
    ATAGAAAAAT TGTATTTGTT GACTCAGTCT CACAACATTC TTATAGAACA GATGATAACT
    TTTGGAGAAA CAATTGAATT GCCTTCATCA AACACTGACT ATGCAAGTCC ATTACTGCCT
    TTGAAAAATA TTTATCTTCC TCACGTCATG TTGTTGGCCA AAACAAAAAT GAGAATTGGA
    CATACAGTGG CCAAGCAAGT ATATTATACC AGTAAAAAGA AGGATTCTTC AAAGTGGTTA
    CCTGCCCTTC ATTTTTTTGA AATAGCTTTG AAGCTCGCTA GGACATCAGG AACAGAAGAA
    CATGAGGTGG AAGCTGAAAT CTTTTTTCAG AAAGGCAAAA TAGAATGTCA AATATTGATG
    GAAGAGAAAC CTCCAACTAT GCAACTTGAG AGTCTGTTTG AAGCTATACA GCTAAGCTTG
    AGAAATGATC AAAATTCAGG GTTGATAAGA GACTCCTACC TTGAAATAGC CTTATTATTT
    TTACACATGA GGAAGTTAAA GAGCAAACTT TCAGTGTCAC CATTGATGAG TATTAAGGCT
    CTTCCCAGAA GGCATAGTTC TATTAAAGAA CCAGTAGTAA ATAAACATGA AATGTACAGT
    TTATTTGCCT GGATTGCAAT AAGAGCTGCT GCACAGGTCA GTGAGGCTGT GCTGGCAATT
    AATCTACTTA TTGGAAAGAA GAATGCTAGA ACTGATACAG TTAGCCAAGG GGCATTATCA
    AATATTCCAG AATTTGCTTC CATGGATCTT TTGTCTTCAT ACACAGATTA TTTGCTTGAT
    AACTACCAAG TTGTCTTCCA GACTGGTTGT ACATTTTCTT ACGTTAGTGA AGAGATACAT
    GAAGATGTAG ATTCTCAGAA AAAAAACCCA ATTAAAGTGG ATATTACATG GATTCTTCTG
    CTGCGCTACT ATATTCACCT ACAAAGAATT AATAATATGA GCAAATTGCT AGCATCTCTG
    AAGCCAGGAT CTGGGATGTC TTTGCCAGAT GACACTCTTC TCACTTCTCT TTTTAACTCT
    GGATTGATTT TGCGCCAAAA AGAAATGCAT TTTTTCCTTA AAAGATTCTT ACAGCTATAT
    TCTTCTTCTT GTATTGATGA ATTTCCAAAA GAACTATTTC AAGTCATGGA ACATCCACCT
    GTTCTAGAAA AAGTCTTATA TGATTCAGCA AGCAAGACAG GAGTAACCAA TGCTTCTCTA
    TGCTCCGATT TATCACTAAA GACCGTTAGC AGCACATTGT ATGGAGACGT GGTGCCACCA
    GTTGTGGCAA CACAGGCTTT AAACAAAGAA CTTTGTTTTC AGTGGTACAT TCCTCCCCTG
    GAAAAACCTC CAAAGGAAAC AGAACCTATG GTTTTATTGT TGTATGCATA TAATATGAAG
    CCCCTGAATA TTTCTGATGT TGCATCTGCT GCTTGCAACT GTGTGTACGT AGGCTCTTTC
    TGGGTTCCAC TAAAAAGAGT TATTTCAGTT CATGAGAAAC TATCTAATCT TACGCAAATT
    GCTGAACTAT CATTGCCAAC AACTCCTAAA GTTACTTCAA ATGAAAATTT ACATGAAGTA
    GAGGAAACAG AAGAGAAATC AATTGATGCA GAAATGGAAA ACATGATTAT TGTCTGTTGC
    AATGAAATAG TATCTTTGTT TTTGGGTGAT GAAATGGCAA CTCCACTATC TGAGGTCCCA
    TTTGATGTCT CGCTGCCATC TATATTCAGT CTGGAGAGAC TTTTTGATCT TGCTAGTGGC
    TGTATCGTAT CGGGAGGAAG CCTTTTCAAC TGGGTGGTAT CAATTATTCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848274.1
    CDS929..10462
    Translation

    Target ORF information:

    RefSeq Version XM_024992506.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992506.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GGAGACAAAG GAGGAGTGAA CAATTTAAAA
    TTTGCTACTG GGAAGGTGGA GTTTTCCATG GGGACAGAAG AGATGCACAT GCCAACACCC
    CCCGACCTTT CTCAGGAGCA CTTCCGAGTT TTCAGCTCAG TGGAGAAGAG CAAGCTTCCC
    TCCTCACAAC TTGGACTCGT AATTTCTTCT TATTATGAAA CTATTAATGT TCTTCAAACC
    AGCAATCAAA GAAGTCTTCA TGTGCAGGCG TTGCATGAAC TTGGAAATCT TCTCATCTTC
    GCACAGAAGA AAAGGGCTGC TTTTAAGTGT TGGAGTCAAG CTCTGGATGA AATTTTCAGA
    AAAGAAGATG TGCTCCATAC ATGGAAGGAG ATTGGCAGCT CACTCACCAG TGCCACTGAT
    GGCTGGTCAC CTCCAGGTTC CAAAGACTAC AGTGAGGAGC TCCTGTCCAA AGGCGGCATC
    TGGGGGTGTT TGCAGGGAGC GGTGATATCA GCAAAGATAG CACAATTTAT TCAGACATTG
    GATGTTGAAA AGAGAACCAA TTGTTGCCTT TTATCAGCAT TACTCTTTCA GGGTTTGCTT
    AGAACCACAC TGCCGCATCC TAAAGCTGAA CGTTGCTACG CTCAGTATGA AATCACTCAG
    CTTCTCCCGG GCATCGAGCT CTTCTCAGAC AGATATCGGG CTGACATCTG CTCCGTCATT
    GCGAGTTTAT ATTACGTCAT CCGCGAGCTG CACTTTGCTA AGCAAAATCT AGTAATTCTG
    CCTCTCCTTG CATTGTACCA ATATTTTGTT TCTGTAATTT GCCAAGACCT AGTCAAAAAT
    CTGGAAGCAA GAATCCTCAA GATAGAAGTC CTTACAGATT TAGGATTCTT TTCCGAAGCC
    TTTCATGAGC TATCCCAGAT ATTTTATGCG AAAAACACAC CTTCTTCAGT ACCCACCAGC
    TGTAAACCTA CTGGAAAAAT GAAGGCACTT GATGATTTAA TAAATAAAGG AATGCCTGTA
    ATTCTGACTA CAGTCAGCCA GCAACATCTC TTAAATAAGT TTTTAGTTGT TAAAGCATAC
    TTTTTCATAA ATGTGGCTGC AACAATAAAT TGTGTCCCAG AACAGACAAT GAAACCAGTC
    GGCCATGGAG TTATGAATGA GAAAAGCAAA CCAAATCTTC CAAACTTGAA AGAGATGTGT
    TTAAAGGATG AGGGAGGCTC ACTTTGTCAT CTGACAAAAT TGAAAGAGAA CTTTGCTCTT
    AGCACACTAA AGTCAATTTT ACTGACAGAA GCTGAAGACA GACTCAACAC CCTTCTGTCG
    GAGATGGACC ATCTAAGCCA TAAGGACCTC TCTCAGTGTT CTGCTGGCGA ACTGGAGATT
    GTAGTGGAGG TCAGACTGCA GCTGGCTGCC ATTGCCCTGC AGAGACACCG GGCAGCATAC
    AGTGCTGCGA TAGTATTTTC CACACTTAAA CTTCTCCAGG ATTCAAAACT TTTTAAAAAG
    AAAGTAGTAG AAGATGACTC AGAGAATTGC ACCTCTTCAG AAAGTTATGC GACTGAAAGT
    AAAGACGACA ATTTTTTAGA TCCTATTTCC TTAAATTCTC GAGAATATTT CAACATTCAT
    CTGTGGTTGA GGTGCCGCTT AGCCTTGGTG ACTGCATTTG TTGCCCAGAT TCGTGGCATT
    GGAATCGTGA AAGAAAACGA TATAACAGAT TACGTGAGCC TTATCAATGA AGTGTGTGTG
    GAGGCAAAAG CTGCAGGTGA CAGGGAGCTA CAGGCTGAAT TCTTGATGCA AGCTGTAATT
    ATCGGCCTAC AAGAAAAGCA TTTAAAGGCA GACATCATAA AAAGCCTTCA GGAGGTAATA
    CAGTTGCTTG AAGGGCATGA CTTTATTTCT CCTCGATCTC ATTTAACCCT GGTGAGAAGC
    ATGCTTTTGC TGGATGATTT AACAAAAGCT GAGAAATTCA AGGAAACTCC TTTTTCGAAA
    ATAGAAAAAT TGTATTTGTT GACTCAGTCT CACAACATTC TTATAGAACA GATGATAACT
    TTTGGAGAAA CAATTGAATT GCCTTCATCA AACACTGACT ATGCAAGTCC ATTACTGCCT
    TTGAAAAATA TTTATCTTCC TCACGTCATG TTGTTGGCCA AAACAAAAAT GAGAATTGGA
    CATACAGTGG CCAAGCAAGT ATATTATACC AGTAAAAAGA AGGATTCTTC AAAGTGGTTA
    CCTGCCCTTC ATTTTTTTGA AATAGCTTTG AAGCTCGCTA GGACATCAGG AACAGAAGAA
    CATGAGGTGG AAGCTGAAAT CTTTTTTCAG AAAGGCAAAA TAGAATGTCA AATATTGATG
    GAAGAGAAAC CTCCAACTAT GCAACTTGAG AGTCTGTTTG AAGCTATACA GCTAAGCTTG
    AGAAATGATC AAAATTCAGG GTTGATAAGA GACTCCTACC TTGAAATAGC CTTATTATTT
    TTACACATGA GGAAGTTAAA GAGCAAACTT TCAGTGTCAC CATTGATGAG TATTAAGGCT
    CTTCCCAGAA GGCATAGTTC TATTAAAGAA CCAGTAGTAA ATAAACATGA AATGTACAGT
    TTATTTGCCT GGATTGCAAT AAGAGCTGCT GCACAGGTCA GTGAGGCTGT GCTGGCAATT
    AATCTACTTA TTGGAAAGAA GAATGCTAGA ACTGATACAG TTAGCCAAGG GGCATTATCA
    AATATTCCAG AATTTGCTTC CATGGATCTT TTGTCTTCAT ACACAGATTA TTTGCTTGAT
    AACTACCAAG TTGTCTTCCA GACTGGTTGT ACATTTTCTT ACGTTAGTGA AGAGATACAT
    GAAGATGTAG ATTCTCAGAA AAAAAACCCA ATTAAAGTGG ATATTACATG GATTCTTCTG
    CTGCGCTACT ATATTCACCT ACAAAGAATT AATAATATGA GCAAATTGCT AGCATCTCTG
    AAGCCAGGAT CTGGGATGTC TTTGCCAGAT GACACTCTTC TCACTTCTCT TTTTAACTCT
    GGATTGATTT TGCGCCAAAA AGAAATGCAT TTTTTCCTTA AAAGATTCTT ACAGCTATAT
    TCTTCTTCTT GTATTGATGA ATTTCCAAAA GAACTATTTC AAGTCATGGA ACATCCACCT
    GTTCTAGAAA AAGTCTTATA TGATTCAGCA AGCAAGACAG GAGTAACCAA TGCTTCTCTA
    TGCTCCGATT TATCACTAAA GACCGTTAGC AGCACATTGT ATGGAGACGT GGTGCCACCA
    GTTGTGGCAA CACAGGCTTT AAACAAAGAA CTTTGTTTTC AGTGGTACAT TCCTCCCCTG
    GAAAAACCTC CAAAGGAAAC AGAACCTATG GTTTTATTGT TGTATGCATA TAATATGAAG
    CCCCTGAATA TTTCTGATGT TGCATCTGCT GCTTGCAACT GTGTGTACGT AGGCTCTTTC
    TGGGTTCCAC TAAAAAGAGT TATTTCAGTT CATGAGAAAC TATCTAATCT TACGCAAATT
    GCTGAACTAT CATTGCCAAC AACTCCTAAA GTTACTTCAA ATGAAAATTT ACATGAAGTA
    GAGGAAACAG AAGAGAAATC AATTGATGCA GAAATGGAAA ACATGATTAT TGTCTGTTGC
    AATGAAATAG TATCTTTGTT TTTGGGTGAT GAAATGGCAA CTCCACTATC TGAGGTCCCA
    TTTGATGTCT CGCTGCCATC TATATTCAGT CTGGAGAGAC TTTTTGATCT TGCTAGTGGC
    TGTATCGTAT CGGGAGGAAG CCTTTTCAAC TGGGTGGTAT CAATTATTCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234799
    Clone ID Related Accession (Same CDS sequence) XM_024992507.1
    Accession Version XM_024992507.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9513bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
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    3721
    3781
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
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    4621
    4681
    4741
    4801
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    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
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    6721
    6781
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    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GGAGACAAAG GAGGAGTGAA CAATTTAAAA
    TTTGCTACTG GGAAGGTGGA GTTTTCCATG GGGACAGAAG AGATGCACAT GCCAACACCC
    CCCGACCTTT CTCAGGAGCA CTTCCGAGTT TTCAGCTCAG TGGAGAAGAG CAAGCTTCCC
    TCCTCACAAC TTGGACTCGT AATTTCTTCT TATTATGAAA CTATTAATGT TCTTCAAACC
    AGCAATCAAA GAAGTCTTCA TGTGCAGGCG TTGCATGAAC TTGGAAATCT TCTCATCTTC
    GCACAGAAGA AAAGGGCTGC TTTTAAGTGT TGGAGTCAAG CTCTGGATGA AATTTTCAGA
    AAAGAAGATG TGCTCCATAC ATGGAAGGAG ATTGGCAGCT CACTCACCAG TGCCACTGAT
    GGCTGGTCAC CTCCAGGTTC CAAAGACTAC AGTGAGGAGC TCCTGTCCAA AGGCGGCATC
    TGGGGGTGTT TGCAGGGAGC GGTGATATCA GCAAAGATAG CACAATTTAT TCAGACATTG
    GATGTTGAAA AGAGAACCAA TTGTTGCCTT TTATCAGCAT TACTCTTTCA GGGTTTGCTT
    AGAACCACAC TGCCGCATCC TAAAGCTGAA CGTTGCTACG CTCAGTATGA AATCACTCAG
    CTTCTCCCGG GCATCGAGCT CTTCTCAGAC AGATATCGGG CTGACATCTG CTCCGTCATT
    GCGAGTTTAT ATTACGTCAT CCGCGAGCTG CACTTTGCTA AGCAAAATCT AGTAATTCTG
    CCTCTCCTTG CATTGTACCA ATATTTTGTT TCTGTAATTT GCCAAGACCT AGTCAAAAAT
    CTGGAAGCAA GAATCCTCAA GATAGAAGTC CTTACAGATT TAGGATTCTT TTCCGAAGCC
    TTTCATGAGC TATCCCAGAT ATTTTATGCG AAAAACACAC CTTCTTCAGT ACCCACCAGC
    TGTAAACCTA CTGGAAAAAT GAAGCTATTT CAATCATTTG ACTCGGGAAA ACCTCTTATT
    AGTAAAGAGA ATCTGCAGGC ACTTGATGAT TTAATAAATA AAGGAATGCC TGTAATTCTG
    ACTACAGTCA GCCAGCAACA TCTCTTAAAT AAGTTTTTAG TTGTTAAAGC ATACTTTTTC
    ATAAATGTGG CTGCAACAAT AAATTGTGTC CCAGAACAGA CAATGAAACC AGTCGGCCAT
    GGAGTTATGA ATGAGAAAAG CAAACCAAAT CTTCCAAACT TGAAAGAGAT GTGTTTAAAG
    GATGAGGGAG GCTCACTTTG TCATCTGACA AAATTGAAAG AGAACTTTGC TCTTAGCACA
    CTAAAGTCAA TTTTACTGAC AGAAGCTGAA GACAGACTCA ACACCCTTCT GTCGGAGATG
    GACCATCTAA GCCATAAGGA CCTCTCTCAG TGTTCTGCTG GCGAACTGGA GATTGTAGTG
    GAGGTCAGAC TGCAGCTGGC TGCCATTGCC CTGCAGAGAC ACCGGGCAGC ATACAGTGCT
    GCGATAGTAT TTTCCACACT TAAACTTCTC CAGGATTCAA AACTTTTTAA AAAGAAAGTA
    GTAGAAGATG ACTCAGAGAA TTGCACCTCT TCAGAAAGTT ATGCGACTGA AAGTAAAGAC
    GACAATTTTT TAGATCCTAT TTCCTTAAAT TCTCGAGAAT ATTTCAACAT TCATCTGTGG
    TTGAGGTGCC GCTTAGCCTT GGTGACTGCA TTTGTTGCCC AGATTCGTGG CATTGGAATC
    GTGAAAGAAA ACGATATAAC AGATTACGTG AGCCTTATCA ATGAAGTGTG TGTGGAGGCA
    AAAGCTGCAG GTGACAGGGA GCTACAGGCT GAATTCTTGA TGCAAGCTGT AATTATCGGC
    CTACAAGAAA AGCATTTAAA GGCAGACATC ATAAAAAGCC TTCAGGAGGT AATACAGTTG
    CTTGAAGGGC ATGACTTTAT TTCTCCTCGA TCTCATTTAA CCCTGGTGAG AAGCATGCTT
    TTGCTGGATG ATTTAACAAA AGCTGAGAAA TTCAAGGAAA CTCCTTTTTC GAAAATAGAA
    AAATTGTATT TGTTGACTCA GTCTCACAAC ATTCTTATAG AACAGATGAT AACTTTTGGA
    GAAACAATTG AATTGCCTTC ATCAAACACT GACTATGCAA GTCCATTACT GCCTTTGAAA
    AATATTTATC TTCCTCACGT CATGTTGTTG GCCAAAACAA AAATGAGAAT TGGACATACA
    GTGGCCAAGC AAGTATATTA TACCAGTAAA AAGAAGGATT CTTCAAAGTG GTTACCTGCC
    CTTCATTTTT TTGAAATAGC TTTGAAGCTC GCTAGGACAT CAGGAACAGA AGAACATGAG
    GTGGAAGCTG AAATCTTTTT TCAGAAAGGC AAAATAGAAT GTCAAATATT GATGGAAGAG
    AAACCTCCAA CTATGCAACT TGAGAGTCTG TTTGAAGCTA TACAGCTAAG CTTGAGAAAT
    GATCAAAATT CAGGGTTGAT AAGAGACTCC TACCTTGAAA TAGCCTTATT ATTTTTACAC
    ATGAGGAAGT TAAAGAGCAA ACTTTCAGTG TCACCATTGA TGAGTATTAA GGCTCTTCCC
    AGAAGGCATA GTTCTATTAA AGAACCAGTA GTAAATAAAC ATGAAATGTA CAGTTTATTT
    GCCTGGATTG CAATAAGAGC TGCTGCACAG GTCAGTGAGG CTGTGCTGGC AATTAATCTA
    CTTATTGGAA AGAAGAATGC TAGAACTGAT ACAGTTAGCC AAGGGGCATT ATCAAATATT
    CCAGAATTTG CTTCCATGGA TCTTTTGTCT TCATACACAG ATTATTTGCT TGATAACTAC
    CAAGTTGTCT TCCAGACTGG TTGTACATTT TCTTACGTTA GTGAAGAGAT ACATGAAGAT
    GTAGATTCTC AGAAAAAAAA CCCAATTAAA GTGGATATTA CATGGATTCT TCTGCTGCGC
    TACTATATTC ACCTACAAAG AATTAATAAT ATGAGCAAAT TGCTAGCATC TCTGAAGCCA
    GGATCTGGGA TGTCTTTGCC AGATGACACT CTTCTCACTT CTCTTTTTAA CTCTGGATTG
    ATTTTGCGCC AAAAAGAAAT GCATTTTTTC CTTAAAAGAT TCTTACAGCT ATATTCTTCT
    TCTTGTATTG ATGAATTTCC AAAAGAACTA TTTCAAGTCA TGGAACATCC ACCTGTTCTA
    GAAAAAGTCT TATATGATTC AGCAAGCAAG ACAGGAGTAA CCAATGCTTC TCTATGCTCC
    GATTTATCAC TAAAGACCGT TAGCAGCACA TTGTATGGAG ACGTGGTGCC ACCAGTTGTG
    GCAACACAGG CTTTAAACAA AGAACTTTGT TTTCAGTGGT ACATTCCTCC CCTGGAAAAA
    CCTCCAAAGG AAACAGAACC TATGGTTTTA TTGTTGTATG CATATAATAT GAAGCCCCTG
    AATATTTCTG ATGTTGCATC TGCTGCTTGC AACTGTGTGT ACGTAGGCTC TTTCTGGGTT
    CCACTAAAAA GAGTTATTTC AGTTCATGAG AAACTATCTA ATCTTACGCA AATTGCTGAA
    CTATCATTGC CAACAACTCC TAAAGTTACT TCAAATGAAA ATTTACATGA AGTAGAGGAA
    ACAGAAGAGA AATCAATTGA TGCAGAAATG GAAGTCCCAT TTGATGTCTC GCTGCCATCT
    ATATTCAGTC TGGAGAGACT TTTTGATCTT GCTAGTGGCT GTATCGTATC GGGAGGAAGC
    CTTTTCAACT GGGTGGTATC AATTATTCCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848275.1
    CDS929..10441
    Translation

    Target ORF information:

    RefSeq Version XM_024992507.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992507.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GGAGACAAAG GAGGAGTGAA CAATTTAAAA
    TTTGCTACTG GGAAGGTGGA GTTTTCCATG GGGACAGAAG AGATGCACAT GCCAACACCC
    CCCGACCTTT CTCAGGAGCA CTTCCGAGTT TTCAGCTCAG TGGAGAAGAG CAAGCTTCCC
    TCCTCACAAC TTGGACTCGT AATTTCTTCT TATTATGAAA CTATTAATGT TCTTCAAACC
    AGCAATCAAA GAAGTCTTCA TGTGCAGGCG TTGCATGAAC TTGGAAATCT TCTCATCTTC
    GCACAGAAGA AAAGGGCTGC TTTTAAGTGT TGGAGTCAAG CTCTGGATGA AATTTTCAGA
    AAAGAAGATG TGCTCCATAC ATGGAAGGAG ATTGGCAGCT CACTCACCAG TGCCACTGAT
    GGCTGGTCAC CTCCAGGTTC CAAAGACTAC AGTGAGGAGC TCCTGTCCAA AGGCGGCATC
    TGGGGGTGTT TGCAGGGAGC GGTGATATCA GCAAAGATAG CACAATTTAT TCAGACATTG
    GATGTTGAAA AGAGAACCAA TTGTTGCCTT TTATCAGCAT TACTCTTTCA GGGTTTGCTT
    AGAACCACAC TGCCGCATCC TAAAGCTGAA CGTTGCTACG CTCAGTATGA AATCACTCAG
    CTTCTCCCGG GCATCGAGCT CTTCTCAGAC AGATATCGGG CTGACATCTG CTCCGTCATT
    GCGAGTTTAT ATTACGTCAT CCGCGAGCTG CACTTTGCTA AGCAAAATCT AGTAATTCTG
    CCTCTCCTTG CATTGTACCA ATATTTTGTT TCTGTAATTT GCCAAGACCT AGTCAAAAAT
    CTGGAAGCAA GAATCCTCAA GATAGAAGTC CTTACAGATT TAGGATTCTT TTCCGAAGCC
    TTTCATGAGC TATCCCAGAT ATTTTATGCG AAAAACACAC CTTCTTCAGT ACCCACCAGC
    TGTAAACCTA CTGGAAAAAT GAAGCTATTT CAATCATTTG ACTCGGGAAA ACCTCTTATT
    AGTAAAGAGA ATCTGCAGGC ACTTGATGAT TTAATAAATA AAGGAATGCC TGTAATTCTG
    ACTACAGTCA GCCAGCAACA TCTCTTAAAT AAGTTTTTAG TTGTTAAAGC ATACTTTTTC
    ATAAATGTGG CTGCAACAAT AAATTGTGTC CCAGAACAGA CAATGAAACC AGTCGGCCAT
    GGAGTTATGA ATGAGAAAAG CAAACCAAAT CTTCCAAACT TGAAAGAGAT GTGTTTAAAG
    GATGAGGGAG GCTCACTTTG TCATCTGACA AAATTGAAAG AGAACTTTGC TCTTAGCACA
    CTAAAGTCAA TTTTACTGAC AGAAGCTGAA GACAGACTCA ACACCCTTCT GTCGGAGATG
    GACCATCTAA GCCATAAGGA CCTCTCTCAG TGTTCTGCTG GCGAACTGGA GATTGTAGTG
    GAGGTCAGAC TGCAGCTGGC TGCCATTGCC CTGCAGAGAC ACCGGGCAGC ATACAGTGCT
    GCGATAGTAT TTTCCACACT TAAACTTCTC CAGGATTCAA AACTTTTTAA AAAGAAAGTA
    GTAGAAGATG ACTCAGAGAA TTGCACCTCT TCAGAAAGTT ATGCGACTGA AAGTAAAGAC
    GACAATTTTT TAGATCCTAT TTCCTTAAAT TCTCGAGAAT ATTTCAACAT TCATCTGTGG
    TTGAGGTGCC GCTTAGCCTT GGTGACTGCA TTTGTTGCCC AGATTCGTGG CATTGGAATC
    GTGAAAGAAA ACGATATAAC AGATTACGTG AGCCTTATCA ATGAAGTGTG TGTGGAGGCA
    AAAGCTGCAG GTGACAGGGA GCTACAGGCT GAATTCTTGA TGCAAGCTGT AATTATCGGC
    CTACAAGAAA AGCATTTAAA GGCAGACATC ATAAAAAGCC TTCAGGAGGT AATACAGTTG
    CTTGAAGGGC ATGACTTTAT TTCTCCTCGA TCTCATTTAA CCCTGGTGAG AAGCATGCTT
    TTGCTGGATG ATTTAACAAA AGCTGAGAAA TTCAAGGAAA CTCCTTTTTC GAAAATAGAA
    AAATTGTATT TGTTGACTCA GTCTCACAAC ATTCTTATAG AACAGATGAT AACTTTTGGA
    GAAACAATTG AATTGCCTTC ATCAAACACT GACTATGCAA GTCCATTACT GCCTTTGAAA
    AATATTTATC TTCCTCACGT CATGTTGTTG GCCAAAACAA AAATGAGAAT TGGACATACA
    GTGGCCAAGC AAGTATATTA TACCAGTAAA AAGAAGGATT CTTCAAAGTG GTTACCTGCC
    CTTCATTTTT TTGAAATAGC TTTGAAGCTC GCTAGGACAT CAGGAACAGA AGAACATGAG
    GTGGAAGCTG AAATCTTTTT TCAGAAAGGC AAAATAGAAT GTCAAATATT GATGGAAGAG
    AAACCTCCAA CTATGCAACT TGAGAGTCTG TTTGAAGCTA TACAGCTAAG CTTGAGAAAT
    GATCAAAATT CAGGGTTGAT AAGAGACTCC TACCTTGAAA TAGCCTTATT ATTTTTACAC
    ATGAGGAAGT TAAAGAGCAA ACTTTCAGTG TCACCATTGA TGAGTATTAA GGCTCTTCCC
    AGAAGGCATA GTTCTATTAA AGAACCAGTA GTAAATAAAC ATGAAATGTA CAGTTTATTT
    GCCTGGATTG CAATAAGAGC TGCTGCACAG GTCAGTGAGG CTGTGCTGGC AATTAATCTA
    CTTATTGGAA AGAAGAATGC TAGAACTGAT ACAGTTAGCC AAGGGGCATT ATCAAATATT
    CCAGAATTTG CTTCCATGGA TCTTTTGTCT TCATACACAG ATTATTTGCT TGATAACTAC
    CAAGTTGTCT TCCAGACTGG TTGTACATTT TCTTACGTTA GTGAAGAGAT ACATGAAGAT
    GTAGATTCTC AGAAAAAAAA CCCAATTAAA GTGGATATTA CATGGATTCT TCTGCTGCGC
    TACTATATTC ACCTACAAAG AATTAATAAT ATGAGCAAAT TGCTAGCATC TCTGAAGCCA
    GGATCTGGGA TGTCTTTGCC AGATGACACT CTTCTCACTT CTCTTTTTAA CTCTGGATTG
    ATTTTGCGCC AAAAAGAAAT GCATTTTTTC CTTAAAAGAT TCTTACAGCT ATATTCTTCT
    TCTTGTATTG ATGAATTTCC AAAAGAACTA TTTCAAGTCA TGGAACATCC ACCTGTTCTA
    GAAAAAGTCT TATATGATTC AGCAAGCAAG ACAGGAGTAA CCAATGCTTC TCTATGCTCC
    GATTTATCAC TAAAGACCGT TAGCAGCACA TTGTATGGAG ACGTGGTGCC ACCAGTTGTG
    GCAACACAGG CTTTAAACAA AGAACTTTGT TTTCAGTGGT ACATTCCTCC CCTGGAAAAA
    CCTCCAAAGG AAACAGAACC TATGGTTTTA TTGTTGTATG CATATAATAT GAAGCCCCTG
    AATATTTCTG ATGTTGCATC TGCTGCTTGC AACTGTGTGT ACGTAGGCTC TTTCTGGGTT
    CCACTAAAAA GAGTTATTTC AGTTCATGAG AAACTATCTA ATCTTACGCA AATTGCTGAA
    CTATCATTGC CAACAACTCC TAAAGTTACT TCAAATGAAA ATTTACATGA AGTAGAGGAA
    ACAGAAGAGA AATCAATTGA TGCAGAAATG GAAGTCCCAT TTGATGTCTC GCTGCCATCT
    ATATTCAGTC TGGAGAGACT TTTTGATCTT GCTAGTGGCT GTATCGTATC GGGAGGAAGC
    CTTTTCAACT GGGTGGTATC AATTATTCCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234800
    Clone ID Related Accession (Same CDS sequence) XM_024992508.1
    Accession Version XM_024992508.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GGAGACAAAG GAGGAGTGAA CAATTTAAAA
    TTTGCTACTG GGAAGGTGGA GTTTTCCATG GGGACAGAAG AGATGCACAT GCCAACACCC
    CCCGACCTTT CTCAGGAGCA CTTCCGAGTT TTCAGCTCAG TGGAGAAGAG CAAGCTTCCC
    TCCTCACAAC TTGGACTCGT AATTTCTTCT TATTATGAAA CTATTAATGT TCTTCAAACC
    AGCAATCAAA GAAGTCTTCA TGTGCAGGCG TTGCATGAAC TTGGAAATCT TCTCATCTTC
    GCACAGAAGA AAAGGGCTGC TTTTAAGTGT TGGAGTCAAG CTCTGGATGA AATTTTCAGA
    AAAGAAGATG TGCTCCATAC ATGGAAGGAG ATTGGCAGCT CACTCACCAG TGCCACTGAT
    GGCTGGTCAC CTCCAGGTTC CAAAGACTAC AGTGAGGAGC TCCTGTCCAA AGGCGGCATC
    TGGGGGTGTT TGCAGGGAGC GGTGATATCA GCAAAGATAG CACAATTTAT TCAGACATTG
    GATGTTGAAA AGAGAACCAA TTGTTGCCTT TTATCAGCAT TACTCTTTCA GGGTTTGCTT
    AGAACCACAC TGCCGCATCC TAAAGCTGAA CGTTGCTACG CTCAGTATGA AATCACTCAG
    CTTCTCCCGG GCATCGAGCT CTTCTCAGAC AGATATCGGG CTGACATCTG CTCCGTCATT
    GCGAGTTTAT ATTACGTCAT CCGCGAGCTG CACTTTGCTA AGCAAAATCT AGTAATTCTG
    CCTCTCCTTG CATTGTACCA ATATTTTGTT TCTGTAATTT GCCAAGACCT AGTCAAAAAT
    CTGGAAGCAA GAATCCTCAA GATAGAAGTC CTTACAGATT TAGGATTCTT TTCCGAAGCC
    TTTCATGAGC TATCCCAGAT ATTTTATGCG AAAAACACAC CTTCTTCAGT ACCCACCAGC
    TGTAAACCTA CTGGAAAAAT GAAGCTATTT CAATCATTTG ACTCGGGAAA ACCTCTTATT
    AGTAAAGAGA ATCTGCAGGC ACTTGATGAT TTAATAAATA AAGGAATGCC TGTAATTCTG
    ACTACAGTCA GCCAGCAACA TCTCTTAAAT AAGTTTTTAG TTGTTAAAGC ATACTTTTTC
    ATAAATGTGG CTGCAACAAT AAATTGTGTC CCAGAACAGA CAATGAAACC AGTCGGCCAT
    GGAGTTATGA ATGAGAAAAG CAAACCAAAT CTTCCAAACT TGAAAGAGAT GTGTTTAAAG
    GATGAGGGAG GCTCACTTTG TCATCTGACA AAATTGAAAG AGAACTTTGC TCTTAGCACA
    CTAAAGTCAA TTTTACTGAC AGAAGCTGAA GACAGACTCA ACACCCTTCT GTCGGAGATG
    GACCATCTAA GCCATAAGGA CCTCTCTCAG TGTTCTGCTG GCGAACTGGA GATTGTAGTG
    GAGGTCAGAC TGCAGCTGGC TGCCATTGCC CTGCAGAGAC ACCGGGCAGC ATACAGTGCT
    GCGATAGTAT TTTCCACACT TAAACTTCTC CAGGATTCAA AACTTTTTAA AAAGAAAGTA
    GTAGAAGATG ACTCAGAGAA TTGCACCTCT TCAGAAAGTT ATGCGACTGA AAGTAAAGAC
    GACAATTTTT TAGATCCTAT TTCCTTAAAT TCTCGAGAAT ATTTCAACAT TCATCTGTGG
    TTGAGGTGCC GCTTAGCCTT GGTGACTGCA TTTGTTGCCC AGATTCGTGG CATTGGAATC
    GTGAAAGAAA ACGATATAAC AGATTACGTG AGCCTTATCA ATGAAGTGTG TGTGGAGGCA
    AAAGCTGCAG GTGACAGGGA GCTACAGGCT GAATTCTTGA TGCAAGCTGT AATTATCGGC
    CTACAAGAAA AGCATTTAAA GGCAGACATC ATAAAAAGCC TTCAGGAGGT AATACAGTTG
    CTTGAAGGGC ATGACTTTAT TTCTCCTCGA TCTCATTTAA CCCTGGTGAG AAGCATGCTT
    TTGCTGGATG ATTTAACAAA AGCTGAGAAA TTCAAGGAAA CTCCTTTTTC GAAAATAGAA
    AAATTGTATT TGTTGACTCA GTCTCACAAC ATTCTTATAG AACAGATGAT AACTTTTGGA
    GAAACAATTG AATTGCCTTC ATCAAACACT GACTATGCAA GTCCATTACT GCCTTTGAAA
    AATATTTATC TTCCTCACGT CATGTTGTTG GCCAAAACAA AAATGAGAAT TGGACATACA
    GTGGCCAAGC AAGTATATTA TACCAGTAAA AAGAAGGATT CTTCAAAGTG GTTACCTGCC
    CTTCATTTTT TTGAAATAGC TTTGAAGCTC GCTAGGACAT CAGGAACAGA AGAACATGAG
    GTGGAAGCTG AAATCTTTTT TCAGAAAGGC AAAATAGAAT GTCAAATATT GATGGAAGAG
    AAACCTCCAA CTATGCAACT TGAGAGTCTG TTTGAAGCTA TACAGCTAAG CTTGAGAAAT
    GATCAAAATT CAGGGTTGAT AAGAGACTCC TACCTTGAAA TAGCCTTATT ATTTTTACAC
    ATGAGGAAGT TAAAGAGCAA ACTTTCAGTG TCACCATTGA TGAGTATTAA GGCTCTTCCC
    AGAAGGCATA GTTCTATTAA AGAACCAGTA GTAAATAAAC ATGAAATGTA CAGTTTATTT
    GCCTGGATTG CAATAAGAGC TGCTGCACAG GTCAGTGAGG CTGTGCTGGC AATTAATCTA
    CTTATTGGAA AGAAGAATGC TAGAACTGAT ACAGTTAGCC AAGGGGCATT ATCAAATATT
    CCAGAATTTG CTTCCATGGA TCTTTTGTCT TCATACACAG ATTATTTGCT TGATAACTAC
    CAAGTTGTCT TCCAGACTGG TTGTACATTT TCTTACGTTA GTGAAGAGAT ACATGAAGAT
    GTAGATTCTC AGAAAAAAAA CCCAATTAAA GTGGATATTA CATGGATTCT TCTGCTGCGC
    TACTATATTC ACCTACAAAG AATTAATAAT ATGAGCAAAT TGCTAGCATC TCTGAAGCCA
    GGATCTGGGA TGTCTTTGCC AGATGACACT CTTCTCACTT CTCTTTTTAA CTCTGGATTG
    ATTTTGCGCC AAAAAGAAAT GCATTTTTTC CTTAAAAGAT TCTTACAGCT ATATTCTTCT
    TCTTGTATTG ATGAATTTCC AAAAGAACTA TTTCAAGTCA TGGAACATCC ACCTGTTCTA
    GAAAAAGTCT TATATGATTC AGCAAGCAAG GTTTTATTGT TGTATGCATA TAATATGAAG
    CCCCTGAATA TTTCTGATGT TGCATCTGCT GCTTGCAACT GTGTGTACGT AGGCTCTTTC
    TGGGTTCCAC TAAAAAGAGT TATTTCAGTT CATGAGAAAC TATCTAATCT TACGCAAATT
    GCTGAACTAT CATTGCCAAC AACTCCTAAA GTTACTTCAA ATGAAAATTT ACATGAAGTA
    GAGGAAACAG AAGAGAAATC AATTGATGCA GAAATGGAAA ACATGATTAT TGTCTGTTGC
    AATGAAATAG TATCTTTGTT TTTGGGTGAT GAAATGGCAA CTCCACTATC TGAGGTCCCA
    TTTGATGTCT CGCTGCCATC TATATTCAGT CTGGAGAGAC TTTTTGATCT TGCTAGTGGC
    TGTATCGTAT CGGGAGGAAG CCTTTTCAAC TGGGTGGTAT CAATTATTCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848276.1
    CDS930..10343
    Translation

    Target ORF information:

    RefSeq Version XM_024992508.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992508.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GGAGACAAAG GAGGAGTGAA CAATTTAAAA
    TTTGCTACTG GGAAGGTGGA GTTTTCCATG GGGACAGAAG AGATGCACAT GCCAACACCC
    CCCGACCTTT CTCAGGAGCA CTTCCGAGTT TTCAGCTCAG TGGAGAAGAG CAAGCTTCCC
    TCCTCACAAC TTGGACTCGT AATTTCTTCT TATTATGAAA CTATTAATGT TCTTCAAACC
    AGCAATCAAA GAAGTCTTCA TGTGCAGGCG TTGCATGAAC TTGGAAATCT TCTCATCTTC
    GCACAGAAGA AAAGGGCTGC TTTTAAGTGT TGGAGTCAAG CTCTGGATGA AATTTTCAGA
    AAAGAAGATG TGCTCCATAC ATGGAAGGAG ATTGGCAGCT CACTCACCAG TGCCACTGAT
    GGCTGGTCAC CTCCAGGTTC CAAAGACTAC AGTGAGGAGC TCCTGTCCAA AGGCGGCATC
    TGGGGGTGTT TGCAGGGAGC GGTGATATCA GCAAAGATAG CACAATTTAT TCAGACATTG
    GATGTTGAAA AGAGAACCAA TTGTTGCCTT TTATCAGCAT TACTCTTTCA GGGTTTGCTT
    AGAACCACAC TGCCGCATCC TAAAGCTGAA CGTTGCTACG CTCAGTATGA AATCACTCAG
    CTTCTCCCGG GCATCGAGCT CTTCTCAGAC AGATATCGGG CTGACATCTG CTCCGTCATT
    GCGAGTTTAT ATTACGTCAT CCGCGAGCTG CACTTTGCTA AGCAAAATCT AGTAATTCTG
    CCTCTCCTTG CATTGTACCA ATATTTTGTT TCTGTAATTT GCCAAGACCT AGTCAAAAAT
    CTGGAAGCAA GAATCCTCAA GATAGAAGTC CTTACAGATT TAGGATTCTT TTCCGAAGCC
    TTTCATGAGC TATCCCAGAT ATTTTATGCG AAAAACACAC CTTCTTCAGT ACCCACCAGC
    TGTAAACCTA CTGGAAAAAT GAAGCTATTT CAATCATTTG ACTCGGGAAA ACCTCTTATT
    AGTAAAGAGA ATCTGCAGGC ACTTGATGAT TTAATAAATA AAGGAATGCC TGTAATTCTG
    ACTACAGTCA GCCAGCAACA TCTCTTAAAT AAGTTTTTAG TTGTTAAAGC ATACTTTTTC
    ATAAATGTGG CTGCAACAAT AAATTGTGTC CCAGAACAGA CAATGAAACC AGTCGGCCAT
    GGAGTTATGA ATGAGAAAAG CAAACCAAAT CTTCCAAACT TGAAAGAGAT GTGTTTAAAG
    GATGAGGGAG GCTCACTTTG TCATCTGACA AAATTGAAAG AGAACTTTGC TCTTAGCACA
    CTAAAGTCAA TTTTACTGAC AGAAGCTGAA GACAGACTCA ACACCCTTCT GTCGGAGATG
    GACCATCTAA GCCATAAGGA CCTCTCTCAG TGTTCTGCTG GCGAACTGGA GATTGTAGTG
    GAGGTCAGAC TGCAGCTGGC TGCCATTGCC CTGCAGAGAC ACCGGGCAGC ATACAGTGCT
    GCGATAGTAT TTTCCACACT TAAACTTCTC CAGGATTCAA AACTTTTTAA AAAGAAAGTA
    GTAGAAGATG ACTCAGAGAA TTGCACCTCT TCAGAAAGTT ATGCGACTGA AAGTAAAGAC
    GACAATTTTT TAGATCCTAT TTCCTTAAAT TCTCGAGAAT ATTTCAACAT TCATCTGTGG
    TTGAGGTGCC GCTTAGCCTT GGTGACTGCA TTTGTTGCCC AGATTCGTGG CATTGGAATC
    GTGAAAGAAA ACGATATAAC AGATTACGTG AGCCTTATCA ATGAAGTGTG TGTGGAGGCA
    AAAGCTGCAG GTGACAGGGA GCTACAGGCT GAATTCTTGA TGCAAGCTGT AATTATCGGC
    CTACAAGAAA AGCATTTAAA GGCAGACATC ATAAAAAGCC TTCAGGAGGT AATACAGTTG
    CTTGAAGGGC ATGACTTTAT TTCTCCTCGA TCTCATTTAA CCCTGGTGAG AAGCATGCTT
    TTGCTGGATG ATTTAACAAA AGCTGAGAAA TTCAAGGAAA CTCCTTTTTC GAAAATAGAA
    AAATTGTATT TGTTGACTCA GTCTCACAAC ATTCTTATAG AACAGATGAT AACTTTTGGA
    GAAACAATTG AATTGCCTTC ATCAAACACT GACTATGCAA GTCCATTACT GCCTTTGAAA
    AATATTTATC TTCCTCACGT CATGTTGTTG GCCAAAACAA AAATGAGAAT TGGACATACA
    GTGGCCAAGC AAGTATATTA TACCAGTAAA AAGAAGGATT CTTCAAAGTG GTTACCTGCC
    CTTCATTTTT TTGAAATAGC TTTGAAGCTC GCTAGGACAT CAGGAACAGA AGAACATGAG
    GTGGAAGCTG AAATCTTTTT TCAGAAAGGC AAAATAGAAT GTCAAATATT GATGGAAGAG
    AAACCTCCAA CTATGCAACT TGAGAGTCTG TTTGAAGCTA TACAGCTAAG CTTGAGAAAT
    GATCAAAATT CAGGGTTGAT AAGAGACTCC TACCTTGAAA TAGCCTTATT ATTTTTACAC
    ATGAGGAAGT TAAAGAGCAA ACTTTCAGTG TCACCATTGA TGAGTATTAA GGCTCTTCCC
    AGAAGGCATA GTTCTATTAA AGAACCAGTA GTAAATAAAC ATGAAATGTA CAGTTTATTT
    GCCTGGATTG CAATAAGAGC TGCTGCACAG GTCAGTGAGG CTGTGCTGGC AATTAATCTA
    CTTATTGGAA AGAAGAATGC TAGAACTGAT ACAGTTAGCC AAGGGGCATT ATCAAATATT
    CCAGAATTTG CTTCCATGGA TCTTTTGTCT TCATACACAG ATTATTTGCT TGATAACTAC
    CAAGTTGTCT TCCAGACTGG TTGTACATTT TCTTACGTTA GTGAAGAGAT ACATGAAGAT
    GTAGATTCTC AGAAAAAAAA CCCAATTAAA GTGGATATTA CATGGATTCT TCTGCTGCGC
    TACTATATTC ACCTACAAAG AATTAATAAT ATGAGCAAAT TGCTAGCATC TCTGAAGCCA
    GGATCTGGGA TGTCTTTGCC AGATGACACT CTTCTCACTT CTCTTTTTAA CTCTGGATTG
    ATTTTGCGCC AAAAAGAAAT GCATTTTTTC CTTAAAAGAT TCTTACAGCT ATATTCTTCT
    TCTTGTATTG ATGAATTTCC AAAAGAACTA TTTCAAGTCA TGGAACATCC ACCTGTTCTA
    GAAAAAGTCT TATATGATTC AGCAAGCAAG GTTTTATTGT TGTATGCATA TAATATGAAG
    CCCCTGAATA TTTCTGATGT TGCATCTGCT GCTTGCAACT GTGTGTACGT AGGCTCTTTC
    TGGGTTCCAC TAAAAAGAGT TATTTCAGTT CATGAGAAAC TATCTAATCT TACGCAAATT
    GCTGAACTAT CATTGCCAAC AACTCCTAAA GTTACTTCAA ATGAAAATTT ACATGAAGTA
    GAGGAAACAG AAGAGAAATC AATTGATGCA GAAATGGAAA ACATGATTAT TGTCTGTTGC
    AATGAAATAG TATCTTTGTT TTTGGGTGAT GAAATGGCAA CTCCACTATC TGAGGTCCCA
    TTTGATGTCT CGCTGCCATC TATATTCAGT CTGGAGAGAC TTTTTGATCT TGCTAGTGGC
    TGTATCGTAT CGGGAGGAAG CCTTTTCAAC TGGGTGGTAT CAATTATTCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234801
    Clone ID Related Accession (Same CDS sequence) XM_024992509.1
    Accession Version XM_024992509.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    541
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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
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    1801
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    1981
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    2641
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    4141
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    4681
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    7141
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    7741
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    7861
    7921
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    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GGAGACAAAG GAGGAGTGAA CAATTTAAAA
    TTTGCTACTG GGAAGGTGGA GTTTTCCATG GGGACAGAAG AGATGCACAT GCCAACACCC
    CCCGACCTTT CTCAGGAGCA CTTCCGAGTT TTCAGCTCAG TGGAGAAGAG CAAGCTTCCC
    TCCTCACAAC TTGGACTCGT AATTTCTTCT TATTATGAAA CTATTAATGT TCTTCAAACC
    AGCAATCAAA GAAGTCTTCA TGTGCAGGCG TTGCATGAAC TTGGAAATCT TCTCATCTTC
    GCACAGAAGA AAAGGGCTGC TTTTAAGTGT TGGAGTCAAG CTCTGGATGA AATTTTCAGA
    AAAGAAGATG TGCTCCATAC ATGGAAGGAG ATTGGCAGCT CACTCACCAG TGCCACTGAT
    GGCTGGTCAC CTCCAGGTTC CAAAGACTAC AGTGAGGAGC TCCTGTCCAA AGGCGGCATC
    TGGGGGTGTT TGCAGGGAGC GGTGATATCA GCAAAGATAG CACAATTTAT TCAGACATTG
    GATGTTGAAA AGAGAACCAA TTGTTGCCTT TTATCAGCAT TACTCTTTCA GGGTTTGCTT
    AGAACCACAC TGCCGCATCC TAAAGCTGAA CGTTGCTACG CTCAGTATGA AATCACTCAG
    CTTCTCCCGG GCATCGAGCT CTTCTCAGAC AGATATCGGG CTGACATCTG CTCCGTCATT
    GCGAGTTTAT ATTACGTCAT CCGCGAGCTG CACTTTGCTA AGCAAAATCT AGTAATTCTG
    CCTCTCCTTG CATTGTACCA ATATTTTGTT TCTGTAATTT GCCAAGACCT AGTCAAAAAT
    CTGGAAGCAA GAATCCTCAA GATAGAAGTC CTTACAGATT TAGGATTCTT TTCCGAAGCC
    TTTCATGAGC TATCCCAGAT ATTTTATGCG AAAAACACAC CTTCTTCAGT ACCCACCAGC
    TGTAAACCTA CTGGAAAAAT GAAGCTATTT CAATCATTTG ACTCGGGAAA ACCTCTTATT
    AGTAAAGAGA ATCTGCAGGC ACTTGATGAT TTAATAAATA AAGGAATGCC TGTAATTCTG
    ACTACAGTCA GCCAGCAACA TCTCTTAAAT AAGTTTTTAG TTGTTAAAGC ATACTTTTTC
    ATAAATGTGG CTGCAACAAT AAATTGTGTC CCAGAACAGA CAATGAAACC AGTCGGCCAT
    GGAGTTATGA ATGAGAAAAG CAAACCAAAT CTTCCAAACT TGAAAGAGAT GTGTTTAAAG
    GATGAGGGAG GCTCACTTTG TCATCTGACA AAATTGAAAG AGAACTTTGC TCTTAGCACA
    CTAAAGTCAA TTTTACTGAC AGAAGCTGAA GACAGACTCA ACACCCTTCT GTCGGAGATG
    GACCATCTAA GCCATAAGGA CCTCTCTCAG TGTTCTGCTG GCGAACTGGA GATTGTAGTG
    GAGGTCAGAC TGCAGCTGGC TGCCATTGCC CTGCAGAGAC ACCGGGCAGC ATACAGTGCT
    GCGATAGTAT TTTCCACACT TAAACTTCTC CAGGATTCAA AACTTTTTAA AAAGAAAGTA
    GTAGAAGATG ACTCAGAGAA TTGCACCTCT TCAGAAAGTT ATGCGACTGA AAGTAAAGAC
    GACAATTTTT TAGATCCTAT TTCCTTAAAT TCTCGAGAAT ATTTCAACAT TCATCTGTGG
    TTGAGGTGCC GCTTAGCCTT GGTGACTGCA TTTGTTGCCC AGATTCGTGG CATTGGAATC
    GTGAAAGAAA ACGATATAAC AGATTACGTG AGCCTTATCA ATGAAGTGTG TGTGGAGGCA
    AAAGCTGCAG GTGACAGGGA GCTACAGGCT GAATTCTTGA TGCAAGCTGT AATTATCGGC
    CTACAAGAAA AGCATTTAAA GGCAGACATC ATAAAAAGCC TTCAGATGAT AACTTTTGGA
    GAAACAATTG AATTGCCTTC ATCAAACACT GACTATGCAA GTCCATTACT GCCTTTGAAA
    AATATTTATC TTCCTCACGT CATGTTGTTG GCCAAAACAA AAATGAGAAT TGGACATACA
    GTGGCCAAGC AAGTATATTA TACCAGTAAA AAGAAGGATT CTTCAAAGTG GTTACCTGCC
    CTTCATTTTT TTGAAATAGC TTTGAAGCTC GCTAGGACAT CAGGAACAGA AGAACATGAG
    GTGGAAGCTG AAATCTTTTT TCAGAAAGGC AAAATAGAAT GTCAAATATT GATGGAAGAG
    AAACCTCCAA CTATGCAACT TGAGAGTCTG TTTGAAGCTA TACAGCTAAG CTTGAGAAAT
    GATCAAAATT CAGGGTTGAT AAGAGACTCC TACCTTGAAA TAGCCTTATT ATTTTTACAC
    ATGAGGAAGT TAAAGAGCAA ACTTTCAGTG TCACCATTGA TGAGTATTAA GGCTCTTCCC
    AGAAGGCATA GTTCTATTAA AGAACCAGTA GTAAATAAAC ATGAAATGTA CAGTTTATTT
    GCCTGGATTG CAATAAGAGC TGCTGCACAG GTCAGTGAGG CTGTGCTGGC AATTAATCTA
    CTTATTGGAA AGAAGAATGC TAGAACTGAT ACAGTTAGCC AAGGGGCATT ATCAAATATT
    CCAGAATTTG CTTCCATGGA TCTTTTGTCT TCATACACAG ATTATTTGCT TGATAACTAC
    CAAGTTGTCT TCCAGACTGG TTGTACATTT TCTTACGTTA GTGAAGAGAT ACATGAAGAT
    GTAGATTCTC AGAAAAAAAA CCCAATTAAA GTGGATATTA CATGGATTCT TCTGCTGCGC
    TACTATATTC ACCTACAAAG AATTAATAAT ATGAGCAAAT TGCTAGCATC TCTGAAGCCA
    GGATCTGGGA TGTCTTTGCC AGATGACACT CTTCTCACTT CTCTTTTTAA CTCTGGATTG
    ATTTTGCGCC AAAAAGAAAT GCATTTTTTC CTTAAAAGAT TCTTACAGCT ATATTCTTCT
    TCTTGTATTG ATGAATTTCC AAAAGAACTA TTTCAAGTCA TGGAACATCC ACCTGTTCTA
    GAAAAAGTCT TATATGATTC AGCAAGCAAG ACAGGAGTAA CCAATGCTTC TCTATGCTCC
    GATTTATCAC TAAAGACCGT TAGCAGCACA TTGTATGGAG ACGTGGTGCC ACCAGTTGTG
    GCAACACAGG CTTTAAACAA AGAACTTTGT TTTCAGTGGT ACATTCCTCC CCTGGAAAAA
    CCTCCAAAGG AAACAGAACC TATGGTTTTA TTGTTGTATG CATATAATAT GAAGCCCCTG
    AATATTTCTG ATGTTGCATC TGCTGCTTGC AACTGTGTGT ACGTAGGCTC TTTCTGGGTT
    CCACTAAAAA GAGTTATTTC AGTTCATGAG AAACTATCTA ATCTTACGCA AATTGCTGAA
    CTATCATTGC CAACAACTCC TAAAGTTACT TCAAATGAAA ATTTACATGA AGTAGAGGAA
    ACAGAAGAGA AATCAATTGA TGCAGAAATG GAAAACATGA TTATTGTCTG TTGCAATGAA
    ATAGTATCTT TGTTTTTGGG TGATGAAATG GCAACTCCAC TATCTGAGGT CCCATTTGAT
    GTCTCGCTGC CATCTATATT CAGTCTGGAG AGACTTTTTG ATCTTGCTAG TGGCTGTATC
    GTATCGGGAG GAAGCCTTTT CAACTGGGTG GTATCAATTA TTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848277.1
    CDS929..10336
    Translation

    Target ORF information:

    RefSeq Version XM_024992509.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992509.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GGAGACAAAG GAGGAGTGAA CAATTTAAAA
    TTTGCTACTG GGAAGGTGGA GTTTTCCATG GGGACAGAAG AGATGCACAT GCCAACACCC
    CCCGACCTTT CTCAGGAGCA CTTCCGAGTT TTCAGCTCAG TGGAGAAGAG CAAGCTTCCC
    TCCTCACAAC TTGGACTCGT AATTTCTTCT TATTATGAAA CTATTAATGT TCTTCAAACC
    AGCAATCAAA GAAGTCTTCA TGTGCAGGCG TTGCATGAAC TTGGAAATCT TCTCATCTTC
    GCACAGAAGA AAAGGGCTGC TTTTAAGTGT TGGAGTCAAG CTCTGGATGA AATTTTCAGA
    AAAGAAGATG TGCTCCATAC ATGGAAGGAG ATTGGCAGCT CACTCACCAG TGCCACTGAT
    GGCTGGTCAC CTCCAGGTTC CAAAGACTAC AGTGAGGAGC TCCTGTCCAA AGGCGGCATC
    TGGGGGTGTT TGCAGGGAGC GGTGATATCA GCAAAGATAG CACAATTTAT TCAGACATTG
    GATGTTGAAA AGAGAACCAA TTGTTGCCTT TTATCAGCAT TACTCTTTCA GGGTTTGCTT
    AGAACCACAC TGCCGCATCC TAAAGCTGAA CGTTGCTACG CTCAGTATGA AATCACTCAG
    CTTCTCCCGG GCATCGAGCT CTTCTCAGAC AGATATCGGG CTGACATCTG CTCCGTCATT
    GCGAGTTTAT ATTACGTCAT CCGCGAGCTG CACTTTGCTA AGCAAAATCT AGTAATTCTG
    CCTCTCCTTG CATTGTACCA ATATTTTGTT TCTGTAATTT GCCAAGACCT AGTCAAAAAT
    CTGGAAGCAA GAATCCTCAA GATAGAAGTC CTTACAGATT TAGGATTCTT TTCCGAAGCC
    TTTCATGAGC TATCCCAGAT ATTTTATGCG AAAAACACAC CTTCTTCAGT ACCCACCAGC
    TGTAAACCTA CTGGAAAAAT GAAGCTATTT CAATCATTTG ACTCGGGAAA ACCTCTTATT
    AGTAAAGAGA ATCTGCAGGC ACTTGATGAT TTAATAAATA AAGGAATGCC TGTAATTCTG
    ACTACAGTCA GCCAGCAACA TCTCTTAAAT AAGTTTTTAG TTGTTAAAGC ATACTTTTTC
    ATAAATGTGG CTGCAACAAT AAATTGTGTC CCAGAACAGA CAATGAAACC AGTCGGCCAT
    GGAGTTATGA ATGAGAAAAG CAAACCAAAT CTTCCAAACT TGAAAGAGAT GTGTTTAAAG
    GATGAGGGAG GCTCACTTTG TCATCTGACA AAATTGAAAG AGAACTTTGC TCTTAGCACA
    CTAAAGTCAA TTTTACTGAC AGAAGCTGAA GACAGACTCA ACACCCTTCT GTCGGAGATG
    GACCATCTAA GCCATAAGGA CCTCTCTCAG TGTTCTGCTG GCGAACTGGA GATTGTAGTG
    GAGGTCAGAC TGCAGCTGGC TGCCATTGCC CTGCAGAGAC ACCGGGCAGC ATACAGTGCT
    GCGATAGTAT TTTCCACACT TAAACTTCTC CAGGATTCAA AACTTTTTAA AAAGAAAGTA
    GTAGAAGATG ACTCAGAGAA TTGCACCTCT TCAGAAAGTT ATGCGACTGA AAGTAAAGAC
    GACAATTTTT TAGATCCTAT TTCCTTAAAT TCTCGAGAAT ATTTCAACAT TCATCTGTGG
    TTGAGGTGCC GCTTAGCCTT GGTGACTGCA TTTGTTGCCC AGATTCGTGG CATTGGAATC
    GTGAAAGAAA ACGATATAAC AGATTACGTG AGCCTTATCA ATGAAGTGTG TGTGGAGGCA
    AAAGCTGCAG GTGACAGGGA GCTACAGGCT GAATTCTTGA TGCAAGCTGT AATTATCGGC
    CTACAAGAAA AGCATTTAAA GGCAGACATC ATAAAAAGCC TTCAGATGAT AACTTTTGGA
    GAAACAATTG AATTGCCTTC ATCAAACACT GACTATGCAA GTCCATTACT GCCTTTGAAA
    AATATTTATC TTCCTCACGT CATGTTGTTG GCCAAAACAA AAATGAGAAT TGGACATACA
    GTGGCCAAGC AAGTATATTA TACCAGTAAA AAGAAGGATT CTTCAAAGTG GTTACCTGCC
    CTTCATTTTT TTGAAATAGC TTTGAAGCTC GCTAGGACAT CAGGAACAGA AGAACATGAG
    GTGGAAGCTG AAATCTTTTT TCAGAAAGGC AAAATAGAAT GTCAAATATT GATGGAAGAG
    AAACCTCCAA CTATGCAACT TGAGAGTCTG TTTGAAGCTA TACAGCTAAG CTTGAGAAAT
    GATCAAAATT CAGGGTTGAT AAGAGACTCC TACCTTGAAA TAGCCTTATT ATTTTTACAC
    ATGAGGAAGT TAAAGAGCAA ACTTTCAGTG TCACCATTGA TGAGTATTAA GGCTCTTCCC
    AGAAGGCATA GTTCTATTAA AGAACCAGTA GTAAATAAAC ATGAAATGTA CAGTTTATTT
    GCCTGGATTG CAATAAGAGC TGCTGCACAG GTCAGTGAGG CTGTGCTGGC AATTAATCTA
    CTTATTGGAA AGAAGAATGC TAGAACTGAT ACAGTTAGCC AAGGGGCATT ATCAAATATT
    CCAGAATTTG CTTCCATGGA TCTTTTGTCT TCATACACAG ATTATTTGCT TGATAACTAC
    CAAGTTGTCT TCCAGACTGG TTGTACATTT TCTTACGTTA GTGAAGAGAT ACATGAAGAT
    GTAGATTCTC AGAAAAAAAA CCCAATTAAA GTGGATATTA CATGGATTCT TCTGCTGCGC
    TACTATATTC ACCTACAAAG AATTAATAAT ATGAGCAAAT TGCTAGCATC TCTGAAGCCA
    GGATCTGGGA TGTCTTTGCC AGATGACACT CTTCTCACTT CTCTTTTTAA CTCTGGATTG
    ATTTTGCGCC AAAAAGAAAT GCATTTTTTC CTTAAAAGAT TCTTACAGCT ATATTCTTCT
    TCTTGTATTG ATGAATTTCC AAAAGAACTA TTTCAAGTCA TGGAACATCC ACCTGTTCTA
    GAAAAAGTCT TATATGATTC AGCAAGCAAG ACAGGAGTAA CCAATGCTTC TCTATGCTCC
    GATTTATCAC TAAAGACCGT TAGCAGCACA TTGTATGGAG ACGTGGTGCC ACCAGTTGTG
    GCAACACAGG CTTTAAACAA AGAACTTTGT TTTCAGTGGT ACATTCCTCC CCTGGAAAAA
    CCTCCAAAGG AAACAGAACC TATGGTTTTA TTGTTGTATG CATATAATAT GAAGCCCCTG
    AATATTTCTG ATGTTGCATC TGCTGCTTGC AACTGTGTGT ACGTAGGCTC TTTCTGGGTT
    CCACTAAAAA GAGTTATTTC AGTTCATGAG AAACTATCTA ATCTTACGCA AATTGCTGAA
    CTATCATTGC CAACAACTCC TAAAGTTACT TCAAATGAAA ATTTACATGA AGTAGAGGAA
    ACAGAAGAGA AATCAATTGA TGCAGAAATG GAAAACATGA TTATTGTCTG TTGCAATGAA
    ATAGTATCTT TGTTTTTGGG TGATGAAATG GCAACTCCAC TATCTGAGGT CCCATTTGAT
    GTCTCGCTGC CATCTATATT CAGTCTGGAG AGACTTTTTG ATCTTGCTAG TGGCTGTATC
    GTATCGGGAG GAAGCCTTTT CAACTGGGTG GTATCAATTA TTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234802
    Clone ID Related Accession (Same CDS sequence) XM_024992510.1
    Accession Version XM_024992510.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGATGCCAT GGCCGCGTAC AGCCACAGAA AAGCTGTTGG AGGAGTTGTT TGATAGCACT
    GCCTCCCGGT TTCTGGCTGT CTTGGAAGCG CTCTCTGACT CAAATCGGCG AATACTACAG
    ACTGGACCTG TTTTTACAGA TGAAGTAGAA GTTCGTGATG TTGTCTCAGA ACTGTTTATG
    GCAGGAAAAG AACTTTTAAT AATGTCAAAT ATTGGTGCAG ATGGCATACT TGATTTTCCA
    AAAGCACATC TTTTGGAACT TATTATAAAA AAGAAGAATC TTATTTCTGT GAATGCTGCC
    GTGAAATTTA TAAAATTAGC TTTCATGTAT GAGGAATGGA GTTTATTTGA ATCTGCGGCT
    GGGCAGCTCA TTGATTTTCT CCAGAGGCAG GATGACCCAG CGTCAAAGAA AGCGGAAAAG
    AATTTAATTC TTCTTGTTGC GATAGAACCT TTGATCAATG TAAAGAGAAA CAAAGGTTTG
    ATCTTTCCTT TTGACAACTA CAAAGAAGGT CAAGTCTATG TAAAAAAAAT TGCTGTTCAT
    GATACTTATG TGAAGTCTTG TGGATACTCA GAAGACATTT TCCATTTGGC GACAACCTTG
    TATTCCTGTG TCTGTGACTC TCCACAGAAT GCTCAGCCGG ATAAGGAAAT CGTTGTGGAC
    ATGATAATGT TCCTGTGGCA GAAATGCAAA TTAGGAATGC AGCGGGTCAC TGTGTCCAAA
    AATGACTACT CAAAGTTTAC CCAGAAGATC AGTACTAATA AATGGCTTTA TCTTCTGTGG
    CAGATAAATG AAATCATTCA CTGCTATAAG ATGGAAGACA TTGATGTTGT GATGGTGGCA
    GAGATCACGT TACGATTAAG TGAAATATTG GAATCTTTAG GAAACCCAAG AAGGAAATTT
    AAAAAATTTC TAGATGTATC TTTAAAGAGA GGGACTAGTG AAATCTTTGG GGGTTCGAAA
    GGGAGCATAG ATATTCTTCC AATAATAAAG AAAAAACCTG AAGAACAGTT ATTTATGGCC
    TATGAACTTC TTGACAAAGC CATTGATGGC ATAAATTTGA ACTGCATGTT AACTGCTTTG
    CCAAATGGAT CATCAGTGAT TGACCATTGC TATGCCACGC ACAACCACTA TATAGATGGA
    GACATGTGCA AACCAGTCAC ACCGAATAAT TTCATAATGG ATTTGCATCT TGAACTAATT
    CAAGCCCAGC ACCGAATAGC TGTTGTGCTT CTTGACCAGT TGCAAGTTTT GCAGACTCCA
    ACTGTGAGCA AAAATACATC TACCAAAGGT ACAGAGAAGC TAAAAAAACC TGCAAGCCCT
    GATTGTTTTA CAGAATTAAG TGTGATGAAT AAAATAAGGA AGAATAAGCT ATCTAAAGCG
    ATTTATTTGA TGCAGAAAAC TCTTCTAATA TTTGAGAAAG ATGCAACCTG TTCTTCTCTA
    CAGAACTACT TGACGGAAGC ATATTCTTTG ATTGAGAAGA CCGAATCAGA ACAAAGTATA
    CAATATTCAT ATCAGAAATA TGTGGAAACT GCCAAAAGAA AGAAAAGCAG GGTCCCTCCT
    CCACCCATCC TGCTATCCCG AACTTACCGT TCTGTGACAT TAAAACCTGC CCCATTTGTT
    TCTGATGTTA AGGTCTGCTG GTACTGCATT TTGGGTCGCA AAGCAGAAGG AAGCTATGGA
    AAAGTAAGAC TAAACGATAA TCATCTCCCA AACTCAGGAG AAGCGATACC AGCAGATGGC
    AAAAGCGTTT TTGAGGTAAA AGGTTTAGAA ACCAATGAAA AATACGTCTT TGCAGTTGCG
    GCTTACTGTT CTAAAGGAAA GCTGATTGGT GATGCTATTG GCGAGACAAC TAAACCAATT
    CTGGTTTACC CACCTCTTTC TGCTGTTACT ACTCGGATGT TATTGACGCA GGTCGCCTAT
    CAGACTGGTA ACTATGATCT GGCTAAGAAA GTTTTCTCAC CAGTCTGGGA TTATTTTGTT
    GCTTCACCAC CTCAGGGTGA CCAATCCATT ATTTGTTTAA GCAATATAAT GACTATTACA
    CAGAGAAGAT TACATTCAGA TATTTTGGCA GAGAGTTCTT CAATTCTCTT ATATCTTTTT
    CTTAGAAATA TTTTTGTAAC AAGTGACATT AAAATTAAAG AAGAAAATTT TTTCTGTGAT
    AATATTAAAG GCAATGAGAT TTTCCCTTCT CAACAAATTG CTAGACTAAT TGAATGTGAG
    AGAGTATTGG TGGCATTGGA ACTGAGCAAC TACCTAAATG ATTCCAGTTA TGCCCTTCAG
    GCTGTGACTC AGTGTTACGG CCTCCTTGCT CCTATCATTT ATCACAATAT TGTTTTGGTA
    CCTGTTGTAC AGATCTTGAT AAAGTGTGTG GTAGTTTTGC AAGGACTACC AAATGTCATC
    CACTCAAAGA AACATCTTGC AAGCTTTGAA AGTGTACAGC ATATGATAGC TTGCTGTATC
    TTCTACATAA CAAAGATCCT GCGTTCATGG AAGGAATATG ACCTGGCAGT AATGATTATA
    AATTATGGAA AAAAGGTGTT GGACATCTCA TCAGGTTGCA AATCTCTATT TGGTACTACA
    GACCTAGAGG AAATGCAAGA AGAGGGTTCT TCCAAGAAGT CTTGCAAGAA TAAGAAGCCA
    CAGCAGGTAT TAGTACCTGA AAAAATAAAT GAACAGCTGG TTTTATTGGA GACACATCTA
    CTCAAACTGA CAAAGCAATA TATTACAACT GAACTCTCAG GAGCAGAAGA TCCCATATTT
    CTTTACCCTA TAGTTTTAAA CTGGTCGGTC AAAGGTGCTG TGAAAGAAGT TATGAAATTT
    AAGCAGAGAC CTAGATTCCT GGAATTCTTT ACACAAGTTA TGCAAAAATG TATGCATGAT
    GAAAAATTTC AACCTATGGT GGAGATAACA AATCCTGTCT ATGACTTCTT GAAAAGGAGG
    AATGAGTCCC TTCTTGGAAT AAAAAAACTG AAATACAAGG ATACTTTGTC TAAAAAGGTC
    ATCAAACCTG TGAAGAAGTA CAGAGCTGCA GTAGTGGAGA TTGGAAAAAC TACAGAAATG
    AAGCAAAAAA AAAAAGCTAA AAGAAAGGAA ACTCTAAGAG AATTTTTGAA TAAAAATCCC
    TTTATTTCTG AAATGCCGGA GCATGAAAGA AATAAGAGAG CTGATGTTCG AAAAGTAGCA
    CATCGATTCC TGGTGGAAAA CTTGAATCCT TTAATATTAA GTTATGTTAA AAAGAAGAGG
    TTCCATCAAA TATTTCTTGA GGAGATGCCC TGGAAAGCTC AGATGAATCT GTATCTGGCA
    AGCGCACACT TCAACTTGCT TTTAACAAAG CTAGGGGAAC GTACAAAGAT AAAATTTGGT
    TCTTCACCGA TAATGGTCAG TTTCCGATCA TGTGACCCTA CTATGTTCTC ACTGTATAAT
    TCAGGAACAG TATTACCAAC AGGAAGAATG ACTCTAGAAA ACTATAAAGT GATGCTTGAT
    TTCCTTCTTA CAGCTAAAAA AAGAAAGGCT CACTTACCAT CAGATGCTGA AGAATTTTTG
    GCATTTCTTA ATTCCAAAAT GAGTGGTGAA AATGTTTCCA AGACCCAAAC GGTTTATGAA
    TCAGACTCTC AGTCGGGACT TAGCAGTAAA GAAAAGGATC GATCTGCAAA TGTGGGTCTC
    TTGGATCATT TTATGAAAAT CTTTTTATAT TGCAGGAGAG CAATGGTTCT TGCTCATCGT
    GGTGGCTATT GGACTCTGCT TCAGAACTGC TGTCGGGCCC TTTGGAACTT TACTCAGGAG
    CTACAAATAC TGCATAAACA AGCAGTGGAT CTGTATAAGA CATTTCCTAT TAGCCAGGAT
    AGTTTCCTCT GTATCTCTGT TTTACCATTC TATTTGGGAG CAGAATTACT TATTGACATG
    TTAATAGAAC TACAGAATAC TGATTCTATT AAGACTATTG ATGAAAAAGG AGAGTTCAGT
    GTCCCGAGTT GCTATGGAAA TATTAAAAAT GATAACGGTG GTTCCAGCCT TACCTTTGAG
    CATCCTCTGG ATGACGTCAA TGTGGTCGAT TTGAAATGGA TCCATGACTT TGTATTAAAA
    TCTCTGGAAC TTGTCTATCA AGTAGAAAAA TGGGAAACAC TCGTATCACT TGCCATTCAG
    TTCAATGCAG TTTCGCATGA AAGATACACA GAGCAAGTGA CTCCACTGCT GGTTTACGCA
    CAGCGCCAGC TTCTACTGCA AATATCCAAA TGCAAGGGCC CAGACATCAG CCAACAGGCT
    TGTGTAAGAT ATGAGACTGA ATACAAACAG AAGATAACCT GCCGAAATTT CATTGGAATA
    CAGCTCAAGA TTAATCCTCC AACCAATAAA GGGACAGCTA TAGGACAGCG AGCAGATGTC
    CTTAGAAAGC TTATCTATTC AGAATACAGC AGAGCCAAAG AGCTCCTCTG TGTGCCTGTG
    GACGTGACAG ACACTCTGAG ATGTTTTAGA GAGACCTTGG AAAAATCCAA ATATCACAAC
    AGATCAATCC GGCACAGCAG AAAGTTGCTT TCGTTATTTC TCGCACAGAC ACAAGGAGAC
    AAAGGAGGAG TGAACAATTT AAAATTTGCT ACTGGGAAGG TGGAGTTTTC CATGGGGACA
    GAAGAGATGC ACATGCCAAC ACCCCCCGAC CTTTCTCAGG AGCACTTCCG AGTTTTCAGC
    TCAGTGGAGA AGAGCAAGCT TCCCTCCTCA CAACTTGGAC TCGTAATTTC TTCTTATTAT
    GAAACTATTA ATGTTCTTCA AACCAGCAAT CAAAGAAGTC TTCATGTGCA GGCGTTGCAT
    GAACTTGGAA ATCTTCTCAT CTTCGCACAG AAGAAAAGGG CTGCTTTTAA GTGTTGGAGT
    CAAGCTCTGG ATGAAATTTT CAGAAAAGAA GATGTGCTCC ATACATGGAA GGAGATTGGC
    AGCTCACTCA CCAGTGCCAC TGATGGCTGG TCACCTCCAG GTTCCAAAGA CTACAGTGAG
    GAGCTCCTGT CCAAAGGCGG CATCTGGGGG TGTTTGCAGG GAGCGGTGAT ATCAGCAAAG
    ATAGCACAAT TTATTCAGAC ATTGGATGTT GAAAAGAGAA CCAATTGTTG CCTTTTATCA
    GCATTACTCT TTCAGGGTTT GCTTAGAACC ACACTGCCGC ATCCTAAAGC TGAACGTTGC
    TACGCTCAGT ATGAAATCAC TCAGCTTCTC CCGGGCATCG AGCTCTTCTC AGACAGATAT
    CGGGCTGACA TCTGCTCCGT CATTGCGAGT TTATATTACG TCATCCGCGA GCTGCACTTT
    GCTAAGCAAA ATCTAGTAAT TCTGCCTCTC CTTGCATTGT ACCAATATTT TGTTTCTGTA
    ATTTGCCAAG ACCTAGTCAA AAATCTGGAA GCAAGAATCC TCAAGATAGA AGTCCTTACA
    GATTTAGGAT TCTTTTCCGA AGCCTTTCAT GAGCTATCCC AGATATTTTA TGCGAAAAAC
    ACACCTTCTT CAGTACCCAC CAGCTGTAAA CCTACTGGAA AAATGAAGCT ATTTCAATCA
    TTTGACTCGG GAAAACCTCT TATTAGTAAA GAGAATCTGC AGGCACTTGA TGATTTAATA
    AATAAAGGAA TGCCTGTAAT TCTGACTACA GTCAGCCAGC AACATCTCTT AAATAAGTTT
    TTAGTTGTTA AAGCATACTT TTTCATAAAT GTGGCTGCAA CAATAAATTG TGTCCCAGAA
    CAGACAATGA AACCAGTCGG CCATGGAGTT ATGAATGAGA AAAGCAAACC AAATCTTCCA
    AACTTGAAAG AGATGTGTTT AAAGGATGAG GGAGGCTCAC TTTGTCATCT GACAAAATTG
    AAAGAGAACT TTGCTCTTAG CACACTAAAG TCAATTTTAC TGACAGAAGC TGAAGACAGA
    CTCAACACCC TTCTGTCGGA GATGGACCAT CTAAGCCATA AGGACCTCTC TCAGTGTTCT
    GCTGGCGAAC TGGAGATTGT AGTGGAGGTC AGACTGCAGC TGGCTGCCAT TGCCCTGCAG
    AGACACCGGG CAGCATACAG TGCTGCGATA GTATTTTCCA CACTTAAACT TCTCCAGGAT
    TCAAAACTTT TTAAAAAGAA AGTAGTAGAA GATGACTCAG AGAATTGCAC CTCTTCAGAA
    AGTTATGCGA CTGAAAGTAA AGACGACAAT TTTTTAGATC CTATTTCCTT AAATTCTCGA
    GAATATTTCA ACATTCATCT GTGGTTGAGG TGCCGCTTAG CCTTGGTGAC TGCATTTGTT
    GCCCAGATTC GTGGCATTGG AATCGTGAAA GAAAACGATA TAACAGATTA CGTGAGCCTT
    ATCAATGAAG TGTGTGTGGA GGCAAAAGCT GCAGGTGACA GGGAGCTACA GGCTGAATTC
    TTGATGCAAG CTGTAATTAT CGGCCTACAA GAAAAGCATT TAAAGGCAGA CATCATAAAA
    AGCCTTCAGG AGGTAATACA GTTGCTTGAA GGGCATGACT TTATTTCTCC TCGATCTCAT
    TTAACCCTGG TGAGAAGCAT GCTTTTGCTG GATGATTTAA CAAAAGCTGA GAAATTCAAG
    GAAACTCCTT TTTCGAAAAT AGAAAAATTG TATTTGTTGA CTCAGTCTCA CAACATTCTT
    ATAGAACAGA TGATAACTTT TGGAGAAACA ATTGAATTGC CTTCATCAAA CACTGACTAT
    GCAAGTCCAT TACTGCCTTT GAAAAATATT TATCTTCCTC ACGTCATGTT GTTGGCCAAA
    ACAAAAATGA GAATTGGACA TACAGTGGCC AAGCAAGTAT ATTATACCAG TAAAAAGAAG
    GATTCTTCAA AGTGGTTACC TGCCCTTCAT TTTTTTGAAA TAGCTTTGAA GCTCGCTAGG
    ACATCAGGAA CAGAAGAACA TGAGGTGGAA GCTGAAATCT TTTTTCAGAA AGGCAAAATA
    GAATGTCAAA TATTGATGGA AGAGAAACCT CCAACTATGC AACTTGAGAG TCTGTTTGAA
    GCTATACAGC TAAGCTTGAG AAATGATCAA AATTCAGGGT TGATAAGAGA CTCCTACCTT
    GAAATAGCCT TATTATTTTT ACACATGAGG AAGTTAAAGA GCAAACTTTC AGTGTCACCA
    TTGATGAGTA TTAAGGCTCT TCCCAGAAGG CATAGTTCTA TTAAAGAACC AGTAGTAAAT
    AAACATGAAA TGTACAGTTT ATTTGCCTGG ATTGCAATAA GAGCTGCTGC ACAGGTCAGT
    GAGGCTGTGC TGGCAATTAA TCTACTTATT GGAAAGAAGA ATGCTAGAAC TGATACAGTT
    AGCCAAGGGG CATTATCAAA TATTCCAGAA TTTGCTTCCA TGGATCTTTT GTCTTCATAC
    ACAGATTATT TGCTTGATAA CTACCAAGTT GTCTTCCAGA CTGGTTGTAC ATTTTCTTAC
    GTTAGTGAAG AGATACATGA AGATGTAGAT TCTCAGAAAA AAAACCCAAT TAAAGTGGAT
    ATTACATGGA TTCTTCTGCT GCGCTACTAT ATTCACCTAC AAAGAATTAA TAATATGAGC
    AAATTGCTAG CATCTCTGAA GCCAGGATCT GGGATGTCTT TGCCAGATGA CACTCTTCTC
    ACTTCTCTTT TTAACTCTGG ATTGATTTTG CGCCAAAAAG AAATGCATTT TTTCCTTAAA
    AGATTCTTAC AGCTATATTC TTCTTCTTGT ATTGATGAAT TTCCAAAAGA ACTATTTCAA
    GTCATGGAAC ATCCACCTGT TCTAGAAAAA GTCTTATATG ATTCAGCAAG CAAGACAGGA
    GTAACCAATG CTTCTCTATG CTCCGATTTA TCACTAAAGA CCGTTAGCAG CACATTGTAT
    GGAGACGTGG TGCCACCAGT TGTGGCAACA CAGGCTTTAA ACAAAGAACT TTGTTTTCAG
    TGGTACATTC CTCCCCTGGA AAAACCTCCA AAGGAAACAG AACCTATGGT TTTATTGTTG
    TATGCATATA ATATGAAGCC CCTGAATATT TCTGATGTTG CATCTGCTGC TTGCAACTGT
    GTGTACGTAG GCTCTTTCTG GGTTCCACTA AAAAGAGTTA TTTCAGTTCA TGAGAAACTA
    TCTAATCTTA CGCAAATTGC TGAACTATCA TTGCCAACAA CTCCTAAAGT TACTTCAAAT
    GAAAATTTAC ATGAAGTAGA GGAAACAGAA GAGAAATCAA TTGATGCAGA AATGGAAAAC
    ATGATTATTG TCTGTTGCAA TGAAATAGTA TCTTTGTTTT TGGGTGATGA AATGGCAACT
    CCACTATCTG AGGTCCCATT TGATGTCTCG CTGCCATCTA TATTCAGTCT GGAGAGACTT
    TTTGATCTTG CTAGTGGCTG TATCGTATCG GGAGGAAGCC TTTTCAACTG GGTGGTATCA
    ATTATTCCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848278.1
    CDS929..10300
    Translation

    Target ORF information:

    RefSeq Version XM_024992510.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992510.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGATGCCAT GGCCGCGTAC AGCCACAGAA AAGCTGTTGG AGGAGTTGTT TGATAGCACT
    GCCTCCCGGT TTCTGGCTGT CTTGGAAGCG CTCTCTGACT CAAATCGGCG AATACTACAG
    ACTGGACCTG TTTTTACAGA TGAAGTAGAA GTTCGTGATG TTGTCTCAGA ACTGTTTATG
    GCAGGAAAAG AACTTTTAAT AATGTCAAAT ATTGGTGCAG ATGGCATACT TGATTTTCCA
    AAAGCACATC TTTTGGAACT TATTATAAAA AAGAAGAATC TTATTTCTGT GAATGCTGCC
    GTGAAATTTA TAAAATTAGC TTTCATGTAT GAGGAATGGA GTTTATTTGA ATCTGCGGCT
    GGGCAGCTCA TTGATTTTCT CCAGAGGCAG GATGACCCAG CGTCAAAGAA AGCGGAAAAG
    AATTTAATTC TTCTTGTTGC GATAGAACCT TTGATCAATG TAAAGAGAAA CAAAGGTTTG
    ATCTTTCCTT TTGACAACTA CAAAGAAGGT CAAGTCTATG TAAAAAAAAT TGCTGTTCAT
    GATACTTATG TGAAGTCTTG TGGATACTCA GAAGACATTT TCCATTTGGC GACAACCTTG
    TATTCCTGTG TCTGTGACTC TCCACAGAAT GCTCAGCCGG ATAAGGAAAT CGTTGTGGAC
    ATGATAATGT TCCTGTGGCA GAAATGCAAA TTAGGAATGC AGCGGGTCAC TGTGTCCAAA
    AATGACTACT CAAAGTTTAC CCAGAAGATC AGTACTAATA AATGGCTTTA TCTTCTGTGG
    CAGATAAATG AAATCATTCA CTGCTATAAG ATGGAAGACA TTGATGTTGT GATGGTGGCA
    GAGATCACGT TACGATTAAG TGAAATATTG GAATCTTTAG GAAACCCAAG AAGGAAATTT
    AAAAAATTTC TAGATGTATC TTTAAAGAGA GGGACTAGTG AAATCTTTGG GGGTTCGAAA
    GGGAGCATAG ATATTCTTCC AATAATAAAG AAAAAACCTG AAGAACAGTT ATTTATGGCC
    TATGAACTTC TTGACAAAGC CATTGATGGC ATAAATTTGA ACTGCATGTT AACTGCTTTG
    CCAAATGGAT CATCAGTGAT TGACCATTGC TATGCCACGC ACAACCACTA TATAGATGGA
    GACATGTGCA AACCAGTCAC ACCGAATAAT TTCATAATGG ATTTGCATCT TGAACTAATT
    CAAGCCCAGC ACCGAATAGC TGTTGTGCTT CTTGACCAGT TGCAAGTTTT GCAGACTCCA
    ACTGTGAGCA AAAATACATC TACCAAAGGT ACAGAGAAGC TAAAAAAACC TGCAAGCCCT
    GATTGTTTTA CAGAATTAAG TGTGATGAAT AAAATAAGGA AGAATAAGCT ATCTAAAGCG
    ATTTATTTGA TGCAGAAAAC TCTTCTAATA TTTGAGAAAG ATGCAACCTG TTCTTCTCTA
    CAGAACTACT TGACGGAAGC ATATTCTTTG ATTGAGAAGA CCGAATCAGA ACAAAGTATA
    CAATATTCAT ATCAGAAATA TGTGGAAACT GCCAAAAGAA AGAAAAGCAG GGTCCCTCCT
    CCACCCATCC TGCTATCCCG AACTTACCGT TCTGTGACAT TAAAACCTGC CCCATTTGTT
    TCTGATGTTA AGGTCTGCTG GTACTGCATT TTGGGTCGCA AAGCAGAAGG AAGCTATGGA
    AAAGTAAGAC TAAACGATAA TCATCTCCCA AACTCAGGAG AAGCGATACC AGCAGATGGC
    AAAAGCGTTT TTGAGGTAAA AGGTTTAGAA ACCAATGAAA AATACGTCTT TGCAGTTGCG
    GCTTACTGTT CTAAAGGAAA GCTGATTGGT GATGCTATTG GCGAGACAAC TAAACCAATT
    CTGGTTTACC CACCTCTTTC TGCTGTTACT ACTCGGATGT TATTGACGCA GGTCGCCTAT
    CAGACTGGTA ACTATGATCT GGCTAAGAAA GTTTTCTCAC CAGTCTGGGA TTATTTTGTT
    GCTTCACCAC CTCAGGGTGA CCAATCCATT ATTTGTTTAA GCAATATAAT GACTATTACA
    CAGAGAAGAT TACATTCAGA TATTTTGGCA GAGAGTTCTT CAATTCTCTT ATATCTTTTT
    CTTAGAAATA TTTTTGTAAC AAGTGACATT AAAATTAAAG AAGAAAATTT TTTCTGTGAT
    AATATTAAAG GCAATGAGAT TTTCCCTTCT CAACAAATTG CTAGACTAAT TGAATGTGAG
    AGAGTATTGG TGGCATTGGA ACTGAGCAAC TACCTAAATG ATTCCAGTTA TGCCCTTCAG
    GCTGTGACTC AGTGTTACGG CCTCCTTGCT CCTATCATTT ATCACAATAT TGTTTTGGTA
    CCTGTTGTAC AGATCTTGAT AAAGTGTGTG GTAGTTTTGC AAGGACTACC AAATGTCATC
    CACTCAAAGA AACATCTTGC AAGCTTTGAA AGTGTACAGC ATATGATAGC TTGCTGTATC
    TTCTACATAA CAAAGATCCT GCGTTCATGG AAGGAATATG ACCTGGCAGT AATGATTATA
    AATTATGGAA AAAAGGTGTT GGACATCTCA TCAGGTTGCA AATCTCTATT TGGTACTACA
    GACCTAGAGG AAATGCAAGA AGAGGGTTCT TCCAAGAAGT CTTGCAAGAA TAAGAAGCCA
    CAGCAGGTAT TAGTACCTGA AAAAATAAAT GAACAGCTGG TTTTATTGGA GACACATCTA
    CTCAAACTGA CAAAGCAATA TATTACAACT GAACTCTCAG GAGCAGAAGA TCCCATATTT
    CTTTACCCTA TAGTTTTAAA CTGGTCGGTC AAAGGTGCTG TGAAAGAAGT TATGAAATTT
    AAGCAGAGAC CTAGATTCCT GGAATTCTTT ACACAAGTTA TGCAAAAATG TATGCATGAT
    GAAAAATTTC AACCTATGGT GGAGATAACA AATCCTGTCT ATGACTTCTT GAAAAGGAGG
    AATGAGTCCC TTCTTGGAAT AAAAAAACTG AAATACAAGG ATACTTTGTC TAAAAAGGTC
    ATCAAACCTG TGAAGAAGTA CAGAGCTGCA GTAGTGGAGA TTGGAAAAAC TACAGAAATG
    AAGCAAAAAA AAAAAGCTAA AAGAAAGGAA ACTCTAAGAG AATTTTTGAA TAAAAATCCC
    TTTATTTCTG AAATGCCGGA GCATGAAAGA AATAAGAGAG CTGATGTTCG AAAAGTAGCA
    CATCGATTCC TGGTGGAAAA CTTGAATCCT TTAATATTAA GTTATGTTAA AAAGAAGAGG
    TTCCATCAAA TATTTCTTGA GGAGATGCCC TGGAAAGCTC AGATGAATCT GTATCTGGCA
    AGCGCACACT TCAACTTGCT TTTAACAAAG CTAGGGGAAC GTACAAAGAT AAAATTTGGT
    TCTTCACCGA TAATGGTCAG TTTCCGATCA TGTGACCCTA CTATGTTCTC ACTGTATAAT
    TCAGGAACAG TATTACCAAC AGGAAGAATG ACTCTAGAAA ACTATAAAGT GATGCTTGAT
    TTCCTTCTTA CAGCTAAAAA AAGAAAGGCT CACTTACCAT CAGATGCTGA AGAATTTTTG
    GCATTTCTTA ATTCCAAAAT GAGTGGTGAA AATGTTTCCA AGACCCAAAC GGTTTATGAA
    TCAGACTCTC AGTCGGGACT TAGCAGTAAA GAAAAGGATC GATCTGCAAA TGTGGGTCTC
    TTGGATCATT TTATGAAAAT CTTTTTATAT TGCAGGAGAG CAATGGTTCT TGCTCATCGT
    GGTGGCTATT GGACTCTGCT TCAGAACTGC TGTCGGGCCC TTTGGAACTT TACTCAGGAG
    CTACAAATAC TGCATAAACA AGCAGTGGAT CTGTATAAGA CATTTCCTAT TAGCCAGGAT
    AGTTTCCTCT GTATCTCTGT TTTACCATTC TATTTGGGAG CAGAATTACT TATTGACATG
    TTAATAGAAC TACAGAATAC TGATTCTATT AAGACTATTG ATGAAAAAGG AGAGTTCAGT
    GTCCCGAGTT GCTATGGAAA TATTAAAAAT GATAACGGTG GTTCCAGCCT TACCTTTGAG
    CATCCTCTGG ATGACGTCAA TGTGGTCGAT TTGAAATGGA TCCATGACTT TGTATTAAAA
    TCTCTGGAAC TTGTCTATCA AGTAGAAAAA TGGGAAACAC TCGTATCACT TGCCATTCAG
    TTCAATGCAG TTTCGCATGA AAGATACACA GAGCAAGTGA CTCCACTGCT GGTTTACGCA
    CAGCGCCAGC TTCTACTGCA AATATCCAAA TGCAAGGGCC CAGACATCAG CCAACAGGCT
    TGTGTAAGAT ATGAGACTGA ATACAAACAG AAGATAACCT GCCGAAATTT CATTGGAATA
    CAGCTCAAGA TTAATCCTCC AACCAATAAA GGGACAGCTA TAGGACAGCG AGCAGATGTC
    CTTAGAAAGC TTATCTATTC AGAATACAGC AGAGCCAAAG AGCTCCTCTG TGTGCCTGTG
    GACGTGACAG ACACTCTGAG ATGTTTTAGA GAGACCTTGG AAAAATCCAA ATATCACAAC
    AGATCAATCC GGCACAGCAG AAAGTTGCTT TCGTTATTTC TCGCACAGAC ACAAGGAGAC
    AAAGGAGGAG TGAACAATTT AAAATTTGCT ACTGGGAAGG TGGAGTTTTC CATGGGGACA
    GAAGAGATGC ACATGCCAAC ACCCCCCGAC CTTTCTCAGG AGCACTTCCG AGTTTTCAGC
    TCAGTGGAGA AGAGCAAGCT TCCCTCCTCA CAACTTGGAC TCGTAATTTC TTCTTATTAT
    GAAACTATTA ATGTTCTTCA AACCAGCAAT CAAAGAAGTC TTCATGTGCA GGCGTTGCAT
    GAACTTGGAA ATCTTCTCAT CTTCGCACAG AAGAAAAGGG CTGCTTTTAA GTGTTGGAGT
    CAAGCTCTGG ATGAAATTTT CAGAAAAGAA GATGTGCTCC ATACATGGAA GGAGATTGGC
    AGCTCACTCA CCAGTGCCAC TGATGGCTGG TCACCTCCAG GTTCCAAAGA CTACAGTGAG
    GAGCTCCTGT CCAAAGGCGG CATCTGGGGG TGTTTGCAGG GAGCGGTGAT ATCAGCAAAG
    ATAGCACAAT TTATTCAGAC ATTGGATGTT GAAAAGAGAA CCAATTGTTG CCTTTTATCA
    GCATTACTCT TTCAGGGTTT GCTTAGAACC ACACTGCCGC ATCCTAAAGC TGAACGTTGC
    TACGCTCAGT ATGAAATCAC TCAGCTTCTC CCGGGCATCG AGCTCTTCTC AGACAGATAT
    CGGGCTGACA TCTGCTCCGT CATTGCGAGT TTATATTACG TCATCCGCGA GCTGCACTTT
    GCTAAGCAAA ATCTAGTAAT TCTGCCTCTC CTTGCATTGT ACCAATATTT TGTTTCTGTA
    ATTTGCCAAG ACCTAGTCAA AAATCTGGAA GCAAGAATCC TCAAGATAGA AGTCCTTACA
    GATTTAGGAT TCTTTTCCGA AGCCTTTCAT GAGCTATCCC AGATATTTTA TGCGAAAAAC
    ACACCTTCTT CAGTACCCAC CAGCTGTAAA CCTACTGGAA AAATGAAGCT ATTTCAATCA
    TTTGACTCGG GAAAACCTCT TATTAGTAAA GAGAATCTGC AGGCACTTGA TGATTTAATA
    AATAAAGGAA TGCCTGTAAT TCTGACTACA GTCAGCCAGC AACATCTCTT AAATAAGTTT
    TTAGTTGTTA AAGCATACTT TTTCATAAAT GTGGCTGCAA CAATAAATTG TGTCCCAGAA
    CAGACAATGA AACCAGTCGG CCATGGAGTT ATGAATGAGA AAAGCAAACC AAATCTTCCA
    AACTTGAAAG AGATGTGTTT AAAGGATGAG GGAGGCTCAC TTTGTCATCT GACAAAATTG
    AAAGAGAACT TTGCTCTTAG CACACTAAAG TCAATTTTAC TGACAGAAGC TGAAGACAGA
    CTCAACACCC TTCTGTCGGA GATGGACCAT CTAAGCCATA AGGACCTCTC TCAGTGTTCT
    GCTGGCGAAC TGGAGATTGT AGTGGAGGTC AGACTGCAGC TGGCTGCCAT TGCCCTGCAG
    AGACACCGGG CAGCATACAG TGCTGCGATA GTATTTTCCA CACTTAAACT TCTCCAGGAT
    TCAAAACTTT TTAAAAAGAA AGTAGTAGAA GATGACTCAG AGAATTGCAC CTCTTCAGAA
    AGTTATGCGA CTGAAAGTAA AGACGACAAT TTTTTAGATC CTATTTCCTT AAATTCTCGA
    GAATATTTCA ACATTCATCT GTGGTTGAGG TGCCGCTTAG CCTTGGTGAC TGCATTTGTT
    GCCCAGATTC GTGGCATTGG AATCGTGAAA GAAAACGATA TAACAGATTA CGTGAGCCTT
    ATCAATGAAG TGTGTGTGGA GGCAAAAGCT GCAGGTGACA GGGAGCTACA GGCTGAATTC
    TTGATGCAAG CTGTAATTAT CGGCCTACAA GAAAAGCATT TAAAGGCAGA CATCATAAAA
    AGCCTTCAGG AGGTAATACA GTTGCTTGAA GGGCATGACT TTATTTCTCC TCGATCTCAT
    TTAACCCTGG TGAGAAGCAT GCTTTTGCTG GATGATTTAA CAAAAGCTGA GAAATTCAAG
    GAAACTCCTT TTTCGAAAAT AGAAAAATTG TATTTGTTGA CTCAGTCTCA CAACATTCTT
    ATAGAACAGA TGATAACTTT TGGAGAAACA ATTGAATTGC CTTCATCAAA CACTGACTAT
    GCAAGTCCAT TACTGCCTTT GAAAAATATT TATCTTCCTC ACGTCATGTT GTTGGCCAAA
    ACAAAAATGA GAATTGGACA TACAGTGGCC AAGCAAGTAT ATTATACCAG TAAAAAGAAG
    GATTCTTCAA AGTGGTTACC TGCCCTTCAT TTTTTTGAAA TAGCTTTGAA GCTCGCTAGG
    ACATCAGGAA CAGAAGAACA TGAGGTGGAA GCTGAAATCT TTTTTCAGAA AGGCAAAATA
    GAATGTCAAA TATTGATGGA AGAGAAACCT CCAACTATGC AACTTGAGAG TCTGTTTGAA
    GCTATACAGC TAAGCTTGAG AAATGATCAA AATTCAGGGT TGATAAGAGA CTCCTACCTT
    GAAATAGCCT TATTATTTTT ACACATGAGG AAGTTAAAGA GCAAACTTTC AGTGTCACCA
    TTGATGAGTA TTAAGGCTCT TCCCAGAAGG CATAGTTCTA TTAAAGAACC AGTAGTAAAT
    AAACATGAAA TGTACAGTTT ATTTGCCTGG ATTGCAATAA GAGCTGCTGC ACAGGTCAGT
    GAGGCTGTGC TGGCAATTAA TCTACTTATT GGAAAGAAGA ATGCTAGAAC TGATACAGTT
    AGCCAAGGGG CATTATCAAA TATTCCAGAA TTTGCTTCCA TGGATCTTTT GTCTTCATAC
    ACAGATTATT TGCTTGATAA CTACCAAGTT GTCTTCCAGA CTGGTTGTAC ATTTTCTTAC
    GTTAGTGAAG AGATACATGA AGATGTAGAT TCTCAGAAAA AAAACCCAAT TAAAGTGGAT
    ATTACATGGA TTCTTCTGCT GCGCTACTAT ATTCACCTAC AAAGAATTAA TAATATGAGC
    AAATTGCTAG CATCTCTGAA GCCAGGATCT GGGATGTCTT TGCCAGATGA CACTCTTCTC
    ACTTCTCTTT TTAACTCTGG ATTGATTTTG CGCCAAAAAG AAATGCATTT TTTCCTTAAA
    AGATTCTTAC AGCTATATTC TTCTTCTTGT ATTGATGAAT TTCCAAAAGA ACTATTTCAA
    GTCATGGAAC ATCCACCTGT TCTAGAAAAA GTCTTATATG ATTCAGCAAG CAAGACAGGA
    GTAACCAATG CTTCTCTATG CTCCGATTTA TCACTAAAGA CCGTTAGCAG CACATTGTAT
    GGAGACGTGG TGCCACCAGT TGTGGCAACA CAGGCTTTAA ACAAAGAACT TTGTTTTCAG
    TGGTACATTC CTCCCCTGGA AAAACCTCCA AAGGAAACAG AACCTATGGT TTTATTGTTG
    TATGCATATA ATATGAAGCC CCTGAATATT TCTGATGTTG CATCTGCTGC TTGCAACTGT
    GTGTACGTAG GCTCTTTCTG GGTTCCACTA AAAAGAGTTA TTTCAGTTCA TGAGAAACTA
    TCTAATCTTA CGCAAATTGC TGAACTATCA TTGCCAACAA CTCCTAAAGT TACTTCAAAT
    GAAAATTTAC ATGAAGTAGA GGAAACAGAA GAGAAATCAA TTGATGCAGA AATGGAAAAC
    ATGATTATTG TCTGTTGCAA TGAAATAGTA TCTTTGTTTT TGGGTGATGA AATGGCAACT
    CCACTATCTG AGGTCCCATT TGATGTCTCG CTGCCATCTA TATTCAGTCT GGAGAGACTT
    TTTGATCTTG CTAGTGGCTG TATCGTATCG GGAGGAAGCC TTTTCAACTG GGTGGTATCA
    ATTATTCCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234803
    Clone ID Related Accession (Same CDS sequence) XM_024992511.1
    Accession Version XM_024992511.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
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    1801
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    1921
    1981
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    4141
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    7141
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    7681
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    8401
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    8521
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    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AATGCTCAGC CGGATAAGGA AATCGTTGTG GACATGATAA TGTTCCTGTG GCAGAAATGC
    AAATTAGGAA TGCAGCGGGT CACTGTGTCC AAAAATGACT ACTCAAAGTT TACCCAGAAG
    ATCAGTACTA ATAAATGGCT TTATCTTCTG TGGCAGATAA ATGAAATCAT TCACTGCTAT
    AAGATGGAAG ACATTGATGT TGTGATGGTG GCAGAGATCA CGTTACGATT AAGTGAAATA
    TTGGAATCTT TAGGAAACCC AAGAAGGAAA TTTAAAAAAT TTCTAGATGT ATCTTTAAAG
    AGAGGGACTA GTGAAATCTT TGGGGGTTCG AAAGGGAGCA TAGATATTCT TCCAATAATA
    AAGAAAAAAC CTGAAGAACA GTTATTTATG GCCTATGAAC TTCTTGACAA AGCCATTGAT
    GGCATAAATT TGAACTGCAT GTTAACTGCT TTGCCAAATG GATCATCAGT GATTGACCAT
    TGCTATGCCA CGCACAACCA CTATATAGAT GGAGACATGT GCAAACCAGT CACACCGAAT
    AATTTCATAA TGGATTTGCA TCTTGAACTA ATTCAAGCCC AGCACCGAAT AGCTGTTGTG
    CTTCTTGACC AGTTGCAAGT TTTGCAGACT CCAACTGTGA GCAAAAATAC ATCTACCAAA
    GGTACAGAGA AGCTAAAAAA ACCTGCAAGC CCTGATTGTT TTACAGAATT AAGTGTGATG
    AATAAAATAA GGAAGAATAA GCTATCTAAA GCGATTTATT TGATGCAGAA AACTCTTCTA
    ATATTTGAGA AAGATGCAAC CTGTTCTTCT CTACAGAACT ACTTGACGGA AGCATATTCT
    TTGATTGAGA AGACCGAATC AGAACAAAGT ATACAATATT CATATCAGAA ATATGTGGAA
    ACTGCCAAAA GAAAGAAAAG CAGGGTCCCT CCTCCACCCA TCCTGCTATC CCGAACTTAC
    CGTTCTGTGA CATTAAAACC TGCCCCATTT GTTTCTGATG TTAAGGTCTG CTGGTACTGC
    ATTTTGGGTC GCAAAGCAGA AGGAAGCTAT GGAAAAGTAA GACTAAACGA TAATCATCTC
    CCAAACTCAG GAGAAGCGAT ACCAGCAGAT GGCAAAAGCG TTTTTGAGGT AAAAGGTTTA
    GAAACCAATG AAAAATACGT CTTTGCAGTT GCGGCTTACT GTTCTAAAGG AAAGCTGATT
    GGTGATGCTA TTGGCGAGAC AACTAAACCA ATTCTGGTTT ACCCACCTCT TTCTGCTGTT
    ACTACTCGGA TGTTATTGAC GCAGGTCGCC TATCAGACTG GTAACTATGA TCTGGCTAAG
    AAAGTTTTCT CACCAGTCTG GGATTATTTT GTTGCTTCAC CACCTCAGGG TGACCAATCC
    ATTATTTGTT TAAGCAATAT AATGACTATT ACACAGAGAA GATTACATTC AGATATTTTG
    GCAGAGAGTT CTTCAATTCT CTTATATCTT TTTCTTAGAA ATATTTTTGT AACAAGTGAC
    ATTAAAATTA AAGAAGAAAA TTTTTTCTGT GATAATATTA AAGGCAATGA GATTTTCCCT
    TCTCAACAAA TTGCTAGACT AATTGAATGT GAGAGAGTAT TGGTGGCATT GGAACTGAGC
    AACTACCTAA ATGATTCCAG TTATGCCCTT CAGGCTGTGA CTCAGTGTTA CGGCCTCCTT
    GCTCCTATCA TTTATCACAA TATTGTTTTG GTACCTGTTG TACAGATCTT GATAAAGTGT
    GTGGTAGTTT TGCAAGGACT ACCAAATGTC ATCCACTCAA AGAAACATCT TGCAAGCTTT
    GAAAGTGTAC AGCATATGAT AGCTTGCTGT ATCTTCTACA TAACAAAGAT CCTGCGTTCA
    TGGAAGGAAT ATGACCTGGC AGTAATGATT ATAAATTATG GAAAAAAGGT GTTGGACATC
    TCATCAGGTT GCAAATCTCT ATTTGGTACT ACAGACCTAG AGGAAATGCA AGAAGAGGGT
    TCTTCCAAGA AGTCTTGCAA GAATAAGAAG CCACAGCAGG TATTAGTACC TGAAAAAATA
    AATGAACAGC TGGTTTTATT GGAGACACAT CTACTCAAAC TGACAAAGCA ATATATTACA
    ACTGAACTCT CAGGAGCAGA AGATCCCATA TTTCTTTACC CTATAGTTTT AAACTGGTCG
    GTCAAAGGTG CTGTGAAAGA AGTTATGAAA TTTAAGCAGA GACCTAGATT CCTGGAATTC
    TTTACACAAG TTATGCAAAA ATGTATGCAT GATGAAAAAT TTCAACCTAT GGTGGAGATA
    ACAAATCCTG TCTATGACTT CTTGAAAAGG AGGAATGAGT CCCTTCTTGG AATAAAAAAA
    CTGAAATACA AGGATACTTT GTCTAAAAAG GTCATCAAAC CTGTGAAGAA GTACAGAGCT
    GCAGTAGTGG AGATTGGAAA AACTACAGAA ATGAAGCAAA AAAAAAAAGC TAAAAGAAAG
    GAAACTCTAA GAGAATTTTT GAATAAAAAT CCCTTTATTT CTGAAATGCC GGAGCATGAA
    AGAAATAAGA GAGCTGATGT TCGAAAAGTA GCACATCGAT TCCTGGTGGA AAACTTGAAT
    CCTTTAATAT TAAGTTATGT TAAAAAGAAG AGGTTCCATC AAATATTTCT TGAGGAGATG
    CCCTGGAAAG CTCAGATGAA TCTGTATCTG GCAAGCGCAC ACTTCAACTT GCTTTTAACA
    AAGCTAGGGG AACGTACAAA GATAAAATTT GGTTCTTCAC CGATAATGGT CAGTTTCCGA
    TCATGTGACC CTACTATGTT CTCACTGTAT AATTCAGGAA CAGTATTACC AACAGGAAGA
    ATGACTCTAG AAAACTATAA AGTGATGCTT GATTTCCTTC TTACAGCTAA AAAAAGAAAG
    GCTCACTTAC CATCAGATGC TGAAGAATTT TTGGCATTTC TTAATTCCAA AATGAGTGGT
    GAAAATGTTT CCAAGACCCA AACGGTTTAT GAATCAGACT CTCAGTCGGG ACTTAGCAGT
    AAAGAAAAGG ATCGATCTGC AAATGTGGGT CTCTTGGATC ATTTTATGAA AATCTTTTTA
    TATTGCAGGA GAGCAATGGT TCTTGCTCAT CGTGGTGGCT ATTGGACTCT GCTTCAGAAC
    TGCTGTCGGG CCCTTTGGAA CTTTACTCAG GAGCTACAAA TACTGCATAA ACAAGCAGTG
    GATCTGTATA AGACATTTCC TATTAGCCAG GATAGTTTCC TCTGTATCTC TGTTTTACCA
    TTCTATTTGG GAGCAGAATT ACTTATTGAC ATGTTAATAG AACTACAGAA TACTGATTCT
    ATTAAGACTA TTGATGAAAA AGGAGAGTTC AGTGTCCCGA GTTGCTATGG AAATATTAAA
    AATGATAACG GTGGTTCCAG CCTTACCTTT GAGCATCCTC TGGATGACGT CAATGTGGTC
    GATTTGAAAT GGATCCATGA CTTTGTATTA AAATCTCTGG AACTTGTCTA TCAAGTAGAA
    AAATGGGAAA CACTCGTATC ACTTGCCATT CAGTTCAATG CAGTTTCGCA TGAAAGATAC
    ACAGAGCAAG TGACTCCACT GCTGGTTTAC GCACAGCGCC AGCTTCTACT GCAAATATCC
    AAATGCAAGG GCCCAGACAT CAGCCAACAG GCTTGTGTAA GATATGAGAC TGAATACAAA
    CAGAAGATAA CCTGCCGAAA TTTCATTGGA ATACAGCTCA AGATTAATCC TCCAACCAAT
    AAAGGGACAG CTATAGGACA GCGAGCAGAT GTCCTTAGAA AGCTTATCTA TTCAGAATAC
    AGCAGAGCCA AAGAGCTCCT CTGTGTGCCT GTGGACGTGA CAGACACTCT GAGATGTTTT
    AGAGAGACCT TGGAAAAATC CAAATATCAC AACAGATCAA TCCGGCACAG CAGAAAGTTG
    CTTTCGTTAT TTCTCGCACA GACACAAGGA GACAAAGGAG GAGTGAACAA TTTAAAATTT
    GCTACTGGGA AGGTGGAGTT TTCCATGGGG ACAGAAGAGA TGCACATGCC AACACCCCCC
    GACCTTTCTC AGGAGCACTT CCGAGTTTTC AGCTCAGTGG AGAAGAGCAA GCTTCCCTCC
    TCACAACTTG GACTCGTAAT TTCTTCTTAT TATGAAACTA TTAATGTTCT TCAAACCAGC
    AATCAAAGAA GTCTTCATGT GCAGGCGTTG CATGAACTTG GAAATCTTCT CATCTTCGCA
    CAGAAGAAAA GGGCTGCTTT TAAGTGTTGG AGTCAAGCTC TGGATGAAAT TTTCAGAAAA
    GAAGATGTGC TCCATACATG GAAGGAGATT GGCAGCTCAC TCACCAGTGC CACTGATGGC
    TGGTCACCTC CAGGTTCCAA AGACTACAGT GAGGAGCTCC TGTCCAAAGG CGGCATCTGG
    GGGTGTTTGC AGGGAGCGGT GATATCAGCA AAGATAGCAC AATTTATTCA GACATTGGAT
    GTTGAAAAGA GAACCAATTG TTGCCTTTTA TCAGCATTAC TCTTTCAGGG TTTGCTTAGA
    ACCACACTGC CGCATCCTAA AGCTGAACGT TGCTACGCTC AGTATGAAAT CACTCAGCTT
    CTCCCGGGCA TCGAGCTCTT CTCAGACAGA TATCGGGCTG ACATCTGCTC CGTCATTGCG
    AGTTTATATT ACGTCATCCG CGAGCTGCAC TTTGCTAAGC AAAATCTAGT AATTCTGCCT
    CTCCTTGCAT TGTACCAATA TTTTGTTTCT GTAATTTGCC AAGACCTAGT CAAAAATCTG
    GAAGCAAGAA TCCTCAAGAT AGAAGTCCTT ACAGATTTAG GATTCTTTTC CGAAGCCTTT
    CATGAGCTAT CCCAGATATT TTATGCGAAA AACACACCTT CTTCAGTACC CACCAGCTGT
    AAACCTACTG GAAAAATGAA GCTATTTCAA TCATTTGACT CGGGAAAACC TCTTATTAGT
    AAAGAGAATC TGCAGGCACT TGATGATTTA ATAAATAAAG GAATGCCTGT AATTCTGACT
    ACAGTCAGCC AGCAACATCT CTTAAATAAG TTTTTAGTTG TTAAAGCATA CTTTTTCATA
    AATGTGGCTG CAACAATAAA TTGTGTCCCA GAACAGACAA TGAAACCAGT CGGCCATGGA
    GTTATGAATG AGAAAAGCAA ACCAAATCTT CCAAACTTGA AAGAGATGTG TTTAAAGGAT
    GAGGGAGGCT CACTTTGTCA TCTGACAAAA TTGAAAGAGA ACTTTGCTCT TAGCACACTA
    AAGTCAATTT TACTGACAGA AGCTGAAGAC AGACTCAACA CCCTTCTGTC GGAGATGGAC
    CATCTAAGCC ATAAGGACCT CTCTCAGTGT TCTGCTGGCG AACTGGAGAT TGTAGTGGAG
    GTCAGACTGC AGCTGGCTGC CATTGCCCTG CAGAGACACC GGGCAGCATA CAGTGCTGCG
    ATAGTATTTT CCACACTTAA ACTTCTCCAG GATTCAAAAC TTTTTAAAAA GAAAGTAGTA
    GAAGATGACT CAGAGAATTG CACCTCTTCA GAAAGTTATG CGACTGAAAG TAAAGACGAC
    AATTTTTTAG ATCCTATTTC CTTAAATTCT CGAGAATATT TCAACATTCA TCTGTGGTTG
    AGGTGCCGCT TAGCCTTGGT GACTGCATTT GTTGCCCAGA TTCGTGGCAT TGGAATCGTG
    AAAGAAAACG ATATAACAGA TTACGTGAGC CTTATCAATG AAGTGTGTGT GGAGGCAAAA
    GCTGCAGGTG ACAGGGAGCT ACAGGCTGAA TTCTTGATGC AAGCTGTAAT TATCGGCCTA
    CAAGAAAAGC ATTTAAAGGC AGACATCATA AAAAGCCTTC AGGAGGTAAT ACAGTTGCTT
    GAAGGGCATG ACTTTATTTC TCCTCGATCT CATTTAACCC TGGTGAGAAG CATGCTTTTG
    CTGGATGATT TAACAAAAGC TGAGAAATTC AAGGAAACTC CTTTTTCGAA AATAGAAAAA
    TTGTATTTGT TGACTCAGTC TCACAACATT CTTATAGAAC AGATGATAAC TTTTGGAGAA
    ACAATTGAAT TGCCTTCATC AAACACTGAC TATGCAAGTC CATTACTGCC TTTGAAAAAT
    ATTTATCTTC CTCACGTCAT GTTGTTGGCC AAAACAAAAA TGAGAATTGG ACATACAGTG
    GCCAAGCAAG TATATTATAC CAGTAAAAAG AAGGATTCTT CAAAGTGGTT ACCTGCCCTT
    CATTTTTTTG AAATAGCTTT GAAGCTCGCT AGGACATCAG GAACAGAAGA ACATGAGGTG
    GAAGCTGAAA TCTTTTTTCA GAAAGGCAAA ATAGAATGTC AAATATTGAT GGAAGAGAAA
    CCTCCAACTA TGCAACTTGA GAGTCTGTTT GAAGCTATAC AGCTAAGCTT GAGAAATGAT
    CAAAATTCAG GGTTGATAAG AGACTCCTAC CTTGAAATAG CCTTATTATT TTTACACATG
    AGGAAGTTAA AGAGCAAACT TTCAGTGTCA CCATTGATGA GTATTAAGGC TCTTCCCAGA
    AGGCATAGTT CTATTAAAGA ACCAGTAGTA AATAAACATG AAATGTACAG TTTATTTGCC
    TGGATTGCAA TAAGAGCTGC TGCACAGGTC AGTGAGGCTG TGCTGGCAAT TAATCTACTT
    ATTGGAAAGA AGAATGCTAG AACTGATACA GTTAGCCAAG GGGCATTATC AAATATTCCA
    GAATTTGCTT CCATGGATCT TTTGTCTTCA TACACAGATT ATTTGCTTGA TAACTACCAA
    GTTGTCTTCC AGACTGGTTG TACATTTTCT TACGTTAGTG AAGAGATACA TGAAGATGTA
    GATTCTCAGA AAAAAAACCC AATTAAAGTG GATATTACAT GGATTCTTCT GCTGCGCTAC
    TATATTCACC TACAAAGAAT TAATAATATG AGCAAATTGC TAGCATCTCT GAAGCCAGGA
    TCTGGGATGT CTTTGCCAGA TGACACTCTT CTCACTTCTC TTTTTAACTC TGGATTGATT
    TTGCGCCAAA AAGAAATGCA TTTTTTCCTT AAAAGATTCT TACAGCTATA TTCTTCTTCT
    TGTATTGATG AATTTCCAAA AGAACTATTT CAAGTCATGG AACATCCACC TGTTCTAGAA
    AAAGTCTTAT ATGATTCAGC AAGCAAGACA GGAGTAACCA ATGCTTCTCT ATGCTCCGAT
    TTATCACTAA AGACCGTTAG CAGCACATTG TATGGAGACG TGGTGCCACC AGTTGTGGCA
    ACACAGGCTT TAAACAAAGA ACTTTGTTTT CAGTGGTACA TTCCTCCCCT GGAAAAACCT
    CCAAAGGAAA CAGAACCTAT GGTTTTATTG TTGTATGCAT ATAATATGAA GCCCCTGAAT
    ATTTCTGATG TTGCATCTGC TGCTTGCAAC TGTGTGTACG TAGGCTCTTT CTGGGTTCCA
    CTAAAAAGAG TTATTTCAGT TCATGAGAAA CTATCTAATC TTACGCAAAT TGCTGAACTA
    TCATTGCCAA CAACTCCTAA AGTTACTTCA AATGAAAATT TACATGAAGT AGAGGAAACA
    GAAGAGAAAT CAATTGATGC AGAAATGGAA AACATGATTA TTGTCTGTTG CAATGAAATA
    GTATCTTTGT TTTTGGGTGA TGAAATGGCA ACTCCACTAT CTGAGGTCCC ATTTGATGTC
    TCGCTGCCAT CTATATTCAG TCTGGAGAGA CTTTTTGATC TTGCTAGTGG CTGTATCGTA
    TCGGGAGGAA GCCTTTTCAA CTGGGTGGTA TCAATTATTC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848279.1
    CDS928..10272
    Translation

    Target ORF information:

    RefSeq Version XM_024992511.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992511.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AATGCTCAGC CGGATAAGGA AATCGTTGTG GACATGATAA TGTTCCTGTG GCAGAAATGC
    AAATTAGGAA TGCAGCGGGT CACTGTGTCC AAAAATGACT ACTCAAAGTT TACCCAGAAG
    ATCAGTACTA ATAAATGGCT TTATCTTCTG TGGCAGATAA ATGAAATCAT TCACTGCTAT
    AAGATGGAAG ACATTGATGT TGTGATGGTG GCAGAGATCA CGTTACGATT AAGTGAAATA
    TTGGAATCTT TAGGAAACCC AAGAAGGAAA TTTAAAAAAT TTCTAGATGT ATCTTTAAAG
    AGAGGGACTA GTGAAATCTT TGGGGGTTCG AAAGGGAGCA TAGATATTCT TCCAATAATA
    AAGAAAAAAC CTGAAGAACA GTTATTTATG GCCTATGAAC TTCTTGACAA AGCCATTGAT
    GGCATAAATT TGAACTGCAT GTTAACTGCT TTGCCAAATG GATCATCAGT GATTGACCAT
    TGCTATGCCA CGCACAACCA CTATATAGAT GGAGACATGT GCAAACCAGT CACACCGAAT
    AATTTCATAA TGGATTTGCA TCTTGAACTA ATTCAAGCCC AGCACCGAAT AGCTGTTGTG
    CTTCTTGACC AGTTGCAAGT TTTGCAGACT CCAACTGTGA GCAAAAATAC ATCTACCAAA
    GGTACAGAGA AGCTAAAAAA ACCTGCAAGC CCTGATTGTT TTACAGAATT AAGTGTGATG
    AATAAAATAA GGAAGAATAA GCTATCTAAA GCGATTTATT TGATGCAGAA AACTCTTCTA
    ATATTTGAGA AAGATGCAAC CTGTTCTTCT CTACAGAACT ACTTGACGGA AGCATATTCT
    TTGATTGAGA AGACCGAATC AGAACAAAGT ATACAATATT CATATCAGAA ATATGTGGAA
    ACTGCCAAAA GAAAGAAAAG CAGGGTCCCT CCTCCACCCA TCCTGCTATC CCGAACTTAC
    CGTTCTGTGA CATTAAAACC TGCCCCATTT GTTTCTGATG TTAAGGTCTG CTGGTACTGC
    ATTTTGGGTC GCAAAGCAGA AGGAAGCTAT GGAAAAGTAA GACTAAACGA TAATCATCTC
    CCAAACTCAG GAGAAGCGAT ACCAGCAGAT GGCAAAAGCG TTTTTGAGGT AAAAGGTTTA
    GAAACCAATG AAAAATACGT CTTTGCAGTT GCGGCTTACT GTTCTAAAGG AAAGCTGATT
    GGTGATGCTA TTGGCGAGAC AACTAAACCA ATTCTGGTTT ACCCACCTCT TTCTGCTGTT
    ACTACTCGGA TGTTATTGAC GCAGGTCGCC TATCAGACTG GTAACTATGA TCTGGCTAAG
    AAAGTTTTCT CACCAGTCTG GGATTATTTT GTTGCTTCAC CACCTCAGGG TGACCAATCC
    ATTATTTGTT TAAGCAATAT AATGACTATT ACACAGAGAA GATTACATTC AGATATTTTG
    GCAGAGAGTT CTTCAATTCT CTTATATCTT TTTCTTAGAA ATATTTTTGT AACAAGTGAC
    ATTAAAATTA AAGAAGAAAA TTTTTTCTGT GATAATATTA AAGGCAATGA GATTTTCCCT
    TCTCAACAAA TTGCTAGACT AATTGAATGT GAGAGAGTAT TGGTGGCATT GGAACTGAGC
    AACTACCTAA ATGATTCCAG TTATGCCCTT CAGGCTGTGA CTCAGTGTTA CGGCCTCCTT
    GCTCCTATCA TTTATCACAA TATTGTTTTG GTACCTGTTG TACAGATCTT GATAAAGTGT
    GTGGTAGTTT TGCAAGGACT ACCAAATGTC ATCCACTCAA AGAAACATCT TGCAAGCTTT
    GAAAGTGTAC AGCATATGAT AGCTTGCTGT ATCTTCTACA TAACAAAGAT CCTGCGTTCA
    TGGAAGGAAT ATGACCTGGC AGTAATGATT ATAAATTATG GAAAAAAGGT GTTGGACATC
    TCATCAGGTT GCAAATCTCT ATTTGGTACT ACAGACCTAG AGGAAATGCA AGAAGAGGGT
    TCTTCCAAGA AGTCTTGCAA GAATAAGAAG CCACAGCAGG TATTAGTACC TGAAAAAATA
    AATGAACAGC TGGTTTTATT GGAGACACAT CTACTCAAAC TGACAAAGCA ATATATTACA
    ACTGAACTCT CAGGAGCAGA AGATCCCATA TTTCTTTACC CTATAGTTTT AAACTGGTCG
    GTCAAAGGTG CTGTGAAAGA AGTTATGAAA TTTAAGCAGA GACCTAGATT CCTGGAATTC
    TTTACACAAG TTATGCAAAA ATGTATGCAT GATGAAAAAT TTCAACCTAT GGTGGAGATA
    ACAAATCCTG TCTATGACTT CTTGAAAAGG AGGAATGAGT CCCTTCTTGG AATAAAAAAA
    CTGAAATACA AGGATACTTT GTCTAAAAAG GTCATCAAAC CTGTGAAGAA GTACAGAGCT
    GCAGTAGTGG AGATTGGAAA AACTACAGAA ATGAAGCAAA AAAAAAAAGC TAAAAGAAAG
    GAAACTCTAA GAGAATTTTT GAATAAAAAT CCCTTTATTT CTGAAATGCC GGAGCATGAA
    AGAAATAAGA GAGCTGATGT TCGAAAAGTA GCACATCGAT TCCTGGTGGA AAACTTGAAT
    CCTTTAATAT TAAGTTATGT TAAAAAGAAG AGGTTCCATC AAATATTTCT TGAGGAGATG
    CCCTGGAAAG CTCAGATGAA TCTGTATCTG GCAAGCGCAC ACTTCAACTT GCTTTTAACA
    AAGCTAGGGG AACGTACAAA GATAAAATTT GGTTCTTCAC CGATAATGGT CAGTTTCCGA
    TCATGTGACC CTACTATGTT CTCACTGTAT AATTCAGGAA CAGTATTACC AACAGGAAGA
    ATGACTCTAG AAAACTATAA AGTGATGCTT GATTTCCTTC TTACAGCTAA AAAAAGAAAG
    GCTCACTTAC CATCAGATGC TGAAGAATTT TTGGCATTTC TTAATTCCAA AATGAGTGGT
    GAAAATGTTT CCAAGACCCA AACGGTTTAT GAATCAGACT CTCAGTCGGG ACTTAGCAGT
    AAAGAAAAGG ATCGATCTGC AAATGTGGGT CTCTTGGATC ATTTTATGAA AATCTTTTTA
    TATTGCAGGA GAGCAATGGT TCTTGCTCAT CGTGGTGGCT ATTGGACTCT GCTTCAGAAC
    TGCTGTCGGG CCCTTTGGAA CTTTACTCAG GAGCTACAAA TACTGCATAA ACAAGCAGTG
    GATCTGTATA AGACATTTCC TATTAGCCAG GATAGTTTCC TCTGTATCTC TGTTTTACCA
    TTCTATTTGG GAGCAGAATT ACTTATTGAC ATGTTAATAG AACTACAGAA TACTGATTCT
    ATTAAGACTA TTGATGAAAA AGGAGAGTTC AGTGTCCCGA GTTGCTATGG AAATATTAAA
    AATGATAACG GTGGTTCCAG CCTTACCTTT GAGCATCCTC TGGATGACGT CAATGTGGTC
    GATTTGAAAT GGATCCATGA CTTTGTATTA AAATCTCTGG AACTTGTCTA TCAAGTAGAA
    AAATGGGAAA CACTCGTATC ACTTGCCATT CAGTTCAATG CAGTTTCGCA TGAAAGATAC
    ACAGAGCAAG TGACTCCACT GCTGGTTTAC GCACAGCGCC AGCTTCTACT GCAAATATCC
    AAATGCAAGG GCCCAGACAT CAGCCAACAG GCTTGTGTAA GATATGAGAC TGAATACAAA
    CAGAAGATAA CCTGCCGAAA TTTCATTGGA ATACAGCTCA AGATTAATCC TCCAACCAAT
    AAAGGGACAG CTATAGGACA GCGAGCAGAT GTCCTTAGAA AGCTTATCTA TTCAGAATAC
    AGCAGAGCCA AAGAGCTCCT CTGTGTGCCT GTGGACGTGA CAGACACTCT GAGATGTTTT
    AGAGAGACCT TGGAAAAATC CAAATATCAC AACAGATCAA TCCGGCACAG CAGAAAGTTG
    CTTTCGTTAT TTCTCGCACA GACACAAGGA GACAAAGGAG GAGTGAACAA TTTAAAATTT
    GCTACTGGGA AGGTGGAGTT TTCCATGGGG ACAGAAGAGA TGCACATGCC AACACCCCCC
    GACCTTTCTC AGGAGCACTT CCGAGTTTTC AGCTCAGTGG AGAAGAGCAA GCTTCCCTCC
    TCACAACTTG GACTCGTAAT TTCTTCTTAT TATGAAACTA TTAATGTTCT TCAAACCAGC
    AATCAAAGAA GTCTTCATGT GCAGGCGTTG CATGAACTTG GAAATCTTCT CATCTTCGCA
    CAGAAGAAAA GGGCTGCTTT TAAGTGTTGG AGTCAAGCTC TGGATGAAAT TTTCAGAAAA
    GAAGATGTGC TCCATACATG GAAGGAGATT GGCAGCTCAC TCACCAGTGC CACTGATGGC
    TGGTCACCTC CAGGTTCCAA AGACTACAGT GAGGAGCTCC TGTCCAAAGG CGGCATCTGG
    GGGTGTTTGC AGGGAGCGGT GATATCAGCA AAGATAGCAC AATTTATTCA GACATTGGAT
    GTTGAAAAGA GAACCAATTG TTGCCTTTTA TCAGCATTAC TCTTTCAGGG TTTGCTTAGA
    ACCACACTGC CGCATCCTAA AGCTGAACGT TGCTACGCTC AGTATGAAAT CACTCAGCTT
    CTCCCGGGCA TCGAGCTCTT CTCAGACAGA TATCGGGCTG ACATCTGCTC CGTCATTGCG
    AGTTTATATT ACGTCATCCG CGAGCTGCAC TTTGCTAAGC AAAATCTAGT AATTCTGCCT
    CTCCTTGCAT TGTACCAATA TTTTGTTTCT GTAATTTGCC AAGACCTAGT CAAAAATCTG
    GAAGCAAGAA TCCTCAAGAT AGAAGTCCTT ACAGATTTAG GATTCTTTTC CGAAGCCTTT
    CATGAGCTAT CCCAGATATT TTATGCGAAA AACACACCTT CTTCAGTACC CACCAGCTGT
    AAACCTACTG GAAAAATGAA GCTATTTCAA TCATTTGACT CGGGAAAACC TCTTATTAGT
    AAAGAGAATC TGCAGGCACT TGATGATTTA ATAAATAAAG GAATGCCTGT AATTCTGACT
    ACAGTCAGCC AGCAACATCT CTTAAATAAG TTTTTAGTTG TTAAAGCATA CTTTTTCATA
    AATGTGGCTG CAACAATAAA TTGTGTCCCA GAACAGACAA TGAAACCAGT CGGCCATGGA
    GTTATGAATG AGAAAAGCAA ACCAAATCTT CCAAACTTGA AAGAGATGTG TTTAAAGGAT
    GAGGGAGGCT CACTTTGTCA TCTGACAAAA TTGAAAGAGA ACTTTGCTCT TAGCACACTA
    AAGTCAATTT TACTGACAGA AGCTGAAGAC AGACTCAACA CCCTTCTGTC GGAGATGGAC
    CATCTAAGCC ATAAGGACCT CTCTCAGTGT TCTGCTGGCG AACTGGAGAT TGTAGTGGAG
    GTCAGACTGC AGCTGGCTGC CATTGCCCTG CAGAGACACC GGGCAGCATA CAGTGCTGCG
    ATAGTATTTT CCACACTTAA ACTTCTCCAG GATTCAAAAC TTTTTAAAAA GAAAGTAGTA
    GAAGATGACT CAGAGAATTG CACCTCTTCA GAAAGTTATG CGACTGAAAG TAAAGACGAC
    AATTTTTTAG ATCCTATTTC CTTAAATTCT CGAGAATATT TCAACATTCA TCTGTGGTTG
    AGGTGCCGCT TAGCCTTGGT GACTGCATTT GTTGCCCAGA TTCGTGGCAT TGGAATCGTG
    AAAGAAAACG ATATAACAGA TTACGTGAGC CTTATCAATG AAGTGTGTGT GGAGGCAAAA
    GCTGCAGGTG ACAGGGAGCT ACAGGCTGAA TTCTTGATGC AAGCTGTAAT TATCGGCCTA
    CAAGAAAAGC ATTTAAAGGC AGACATCATA AAAAGCCTTC AGGAGGTAAT ACAGTTGCTT
    GAAGGGCATG ACTTTATTTC TCCTCGATCT CATTTAACCC TGGTGAGAAG CATGCTTTTG
    CTGGATGATT TAACAAAAGC TGAGAAATTC AAGGAAACTC CTTTTTCGAA AATAGAAAAA
    TTGTATTTGT TGACTCAGTC TCACAACATT CTTATAGAAC AGATGATAAC TTTTGGAGAA
    ACAATTGAAT TGCCTTCATC AAACACTGAC TATGCAAGTC CATTACTGCC TTTGAAAAAT
    ATTTATCTTC CTCACGTCAT GTTGTTGGCC AAAACAAAAA TGAGAATTGG ACATACAGTG
    GCCAAGCAAG TATATTATAC CAGTAAAAAG AAGGATTCTT CAAAGTGGTT ACCTGCCCTT
    CATTTTTTTG AAATAGCTTT GAAGCTCGCT AGGACATCAG GAACAGAAGA ACATGAGGTG
    GAAGCTGAAA TCTTTTTTCA GAAAGGCAAA ATAGAATGTC AAATATTGAT GGAAGAGAAA
    CCTCCAACTA TGCAACTTGA GAGTCTGTTT GAAGCTATAC AGCTAAGCTT GAGAAATGAT
    CAAAATTCAG GGTTGATAAG AGACTCCTAC CTTGAAATAG CCTTATTATT TTTACACATG
    AGGAAGTTAA AGAGCAAACT TTCAGTGTCA CCATTGATGA GTATTAAGGC TCTTCCCAGA
    AGGCATAGTT CTATTAAAGA ACCAGTAGTA AATAAACATG AAATGTACAG TTTATTTGCC
    TGGATTGCAA TAAGAGCTGC TGCACAGGTC AGTGAGGCTG TGCTGGCAAT TAATCTACTT
    ATTGGAAAGA AGAATGCTAG AACTGATACA GTTAGCCAAG GGGCATTATC AAATATTCCA
    GAATTTGCTT CCATGGATCT TTTGTCTTCA TACACAGATT ATTTGCTTGA TAACTACCAA
    GTTGTCTTCC AGACTGGTTG TACATTTTCT TACGTTAGTG AAGAGATACA TGAAGATGTA
    GATTCTCAGA AAAAAAACCC AATTAAAGTG GATATTACAT GGATTCTTCT GCTGCGCTAC
    TATATTCACC TACAAAGAAT TAATAATATG AGCAAATTGC TAGCATCTCT GAAGCCAGGA
    TCTGGGATGT CTTTGCCAGA TGACACTCTT CTCACTTCTC TTTTTAACTC TGGATTGATT
    TTGCGCCAAA AAGAAATGCA TTTTTTCCTT AAAAGATTCT TACAGCTATA TTCTTCTTCT
    TGTATTGATG AATTTCCAAA AGAACTATTT CAAGTCATGG AACATCCACC TGTTCTAGAA
    AAAGTCTTAT ATGATTCAGC AAGCAAGACA GGAGTAACCA ATGCTTCTCT ATGCTCCGAT
    TTATCACTAA AGACCGTTAG CAGCACATTG TATGGAGACG TGGTGCCACC AGTTGTGGCA
    ACACAGGCTT TAAACAAAGA ACTTTGTTTT CAGTGGTACA TTCCTCCCCT GGAAAAACCT
    CCAAAGGAAA CAGAACCTAT GGTTTTATTG TTGTATGCAT ATAATATGAA GCCCCTGAAT
    ATTTCTGATG TTGCATCTGC TGCTTGCAAC TGTGTGTACG TAGGCTCTTT CTGGGTTCCA
    CTAAAAAGAG TTATTTCAGT TCATGAGAAA CTATCTAATC TTACGCAAAT TGCTGAACTA
    TCATTGCCAA CAACTCCTAA AGTTACTTCA AATGAAAATT TACATGAAGT AGAGGAAACA
    GAAGAGAAAT CAATTGATGC AGAAATGGAA AACATGATTA TTGTCTGTTG CAATGAAATA
    GTATCTTTGT TTTTGGGTGA TGAAATGGCA ACTCCACTAT CTGAGGTCCC ATTTGATGTC
    TCGCTGCCAT CTATATTCAG TCTGGAGAGA CTTTTTGATC TTGCTAGTGG CTGTATCGTA
    TCGGGAGGAA GCCTTTTCAA CTGGGTGGTA TCAATTATTC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234804
    Clone ID Related Accession (Same CDS sequence) XM_024992512.1
    Accession Version XM_024992512.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8817bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    ATGCAGGTGG CTTTGCCACA GGAGCACCTG TGCTGGATTA TCTTCAATGG TACCATTTAC 
    ATTTACACTA TTTGCAGAAA ATTGATGATC AGAGGTCATT CTTCCAAGGC CTTGGAATAC
    CTCCTGTGGG CCAGTATATG TATGGAGTCT TCAGTGCCTC TCCTGTCCAT CAGGTACTTG
    ACGTGGAGAG CTACTCTTTA CACAGCTGTC TGCCAGTGCT ACTATGACTG CCAAGCTGGT
    GTTCATGGAG AGGCATTTGC TCGGCGTGCT TTAGCTAAAA TTGATGAGTT ACGGCAACTG
    GAATTAATGA GTTCCTCACA ACCACAGGAA GAATCCAGAG AATATTATAG AGAGGCCACA
    ATAAAGATGG CAGTCATGAT CTTCAAAAGA GGAGTATTTG AATCTAGAAG AAAAAACAAA
    AGTTTCTTTA GACCAAAGAT AAGAGTTAAC ATAAAGGAAG CACAAACTAT GCCATGGCCG
    CGTACAGCCA CAGAAAAGCT GTTGGAGGAG TTGTTTGATA GCACTGCCTC CCGGTTTCTG
    GCTGTCTTGG AAGCGCTCTC TGACTCAAAT CGGCGAATAC TACAGACTGG ACCTGTTTTT
    ACAGATGAAG TAGAAGTTCG TGATGTTGTC TCAGAACTGT TTATGGCAGG AAAAGAACTT
    TTAATAATGT CAAATATTGG TGCAGATGGC ATACTTGATT TTCCAAAAGC ACATCTTTTG
    GAACTTATTA TAAAAAAGAA GAATCTTATT TCTGTGAATG CTGCCGTGAA ATTTATAAAA
    TTAGCTTTCA TGTATGAGGA ATGGAGTTTA TTTGAATCTG CGGCTGGGCA GCTCATTGAT
    TTTCTCCAGA GGCAGGATGA CCCAGCGTCA AAGAAAGCGG AAAAGAATTT AATTCTTCTT
    GTTGCGATAG AACCTTTGAT CAATGTAAAG AGAAACAAAG GTTTGATCTT TCCTTTTGAC
    AACTACAAAG AAGGTCAAGT CTATGTAAAA AAAATTGCTG TTCATGATAC TTATGTGAAG
    TCTTGTGGAT ACTCAGAAGA CATTTTCCAT TTGGCGACAA CCTTGTATTC CTGTGTCTGT
    GACTCTCCAC AGAATGCTCA GCCGGATAAG GAAATCGTTG TGGACATGAT AATGTTCCTG
    TGGCAGAAAT GCAAATTAGG AATGCAGCGG GTCACTGTGT CCAAAAATGA CTACTCAAAG
    TTTACCCAGA AGATCAGTAC TAATAAATGG CTTTATCTTC TGTGGCAGAT AAATGAAATC
    ATTCACTGCT ATAAGATGGA AGACATTGAT GTTGTGATGG TGGCAGAGAT CACGTTACGA
    TTAAGTGAAA TATTGGAATC TTTAGGAAAC CCAAGAAGGA AATTTAAAAA ATTTCTAGAT
    GTATCTTTAA AGAGAGGGAC TAGTGAAATC TTTGGGGGTT CGAAAGGGAG CATAGATATT
    CTTCCAATAA TAAAGAAAAA ACCTGAAGAA CAGTTATTTA TGGCCTATGA ACTTCTTGAC
    AAAGCCATTG ATGGCATAAA TTTGAACTGC ATGTTAACTG CTTTGCCAAA TGGATCATCA
    GTGATTGACC ATTGCTATGC CACGCACAAC CACTATATAG ATGGAGACAT GTGCAAACCA
    GTCACACCGA ATAATTTCAT AATGGATTTG CATCTTGAAC TAATTCAAGC CCAGCACCGA
    ATAGCTGTTG TGCTTCTTGA CCAGTTGCAA GTTTTGCAGA CTCCAACTGT GAGCAAAAAT
    ACATCTACCA AAGGTACAGA GAAGCTAAAA AAACCTGCAA GCCCTGATTG TTTTACAGAA
    TTAAGTGTGA TGAATAAAAT AAGGAAGAAT AAGCTATCTA AAGCGATTTA TTTGATGCAG
    AAAACTCTTC TAATATTTGA GAAAGATGCA ACCTGTTCTT CTCTACAGAA CTACTTGACG
    GAAGCATATT CTTTGATTGA GAAGACCGAA TCAGAACAAA GTATACAATA TTCATATCAG
    AAATATGTGG AAACTGCCAA AAGAAAGAAA AGCAGGGTCC CTCCTCCACC CATCCTGCTA
    TCCCGAACTT ACCGTTCTGT GACATTAAAA CCTGCCCCAT TTGTTTCTGA TGTTAAGGTC
    TGCTGGTACT GCATTTTGGG TCGCAAAGCA GAAGGAAGCT ATGGAAAAGT AAGACTAAAC
    GATAATCATC TCCCAAACTC AGGAGAAGCG ATACCAGCAG ATGGCAAAAG CGTTTTTGAG
    GTAAAAGGTT TAGAAACCAA TGAAAAATAC GTCTTTGCAG TTGCGGCTTA CTGTTCTAAA
    GGAAAGCTGA TTGGTGATGC TATTGGCGAG ACAACTAAAC CAATTCTGGT TTACCCACCT
    CTTTCTGCTG TTACTACTCG GATGTTATTG ACGCAGGTCG CCTATCAGAC TGGTAACTAT
    GATCTGGCTA AGAAAGTTTT CTCACCAGTC TGGGATTATT TTGTTGCTTC ACCACCTCAG
    GGTGACCAAT CCATTATTTG TTTAAGCAAT ATAATGACTA TTACACAGAG AAGATTACAT
    TCAGATATTT TGGCAGAGAG TTCTTCAATT CTCTTATATC TTTTTCTTAG AAATATTTTT
    GTAACAAGTG ACATTAAAAT TAAAGAAGAA AATTTTTTCT GTGATAATAT TAAAGGCAAT
    GAGATTTTCC CTTCTCAACA AATTGCTAGA CTAATTGAAT GTGAGAGAGT ATTGGTGGCA
    TTGGAACTGA GCAACTACCT AAATGATTCC AGTTATGCCC TTCAGGCTGT GACTCAGTGT
    TACGGCCTCC TTGCTCCTAT CATTTATCAC AATATTGTTT TGGTACCTGT TGTACAGATC
    TTGATAAAGT GTGTGGTAGT TTTGCAAGGA CTACCAAATG TCATCCACTC AAAGAAACAT
    CTTGCAAGCT TTGAAAGTGT ACAGCATATG ATAGCTTGCT GTATCTTCTA CATAACAAAG
    ATCCTGCGTT CATGGAAGGA ATATGACCTG GCAGTAATGA TTATAAATTA TGGAAAAAAG
    GTGTTGGACA TCTCATCAGG TTGCAAATCT CTATTTGGTA CTACAGACCT AGAGGAAATG
    CAAGAAGAGG GTTCTTCCAA GAAGTCTTGC AAGAATAAGA AGCCACAGCA GGTATTAGTA
    CCTGAAAAAA TAAATGAACA GCTGGTTTTA TTGGAGACAC ATCTACTCAA ACTGACAAAG
    CAATATATTA CAACTGAACT CTCAGGAGCA GAAGATCCCA TATTTCTTTA CCCTATAGTT
    TTAAACTGGT CGGTCAAAGG TGCTGTGAAA GAAGTTATGA AATTTAAGCA GAGACCTAGA
    TTCCTGGAAT TCTTTACACA AGTTATGCAA AAATGTATGC ATGATGAAAA ATTTCAACCT
    ATGGTGGAGA TAACAAATCC TGTCTATGAC TTCTTGAAAA GGAGGAATGA GTCCCTTCTT
    GGAATAAAAA AACTGAAATA CAAGGATACT TTGTCTAAAA AGGTCATCAA ACCTGTGAAG
    AAGTACAGAG CTGCAGTAGT GGAGATTGGA AAAACTACAG AAATGAAGCA AAAAAAAAAA
    GCTAAAAGAA AGGAAACTCT AAGAGAATTT TTGAATAAAA ATCCCTTTAT TTCTGAAATG
    CCGGAGCATG AAAGAAATAA GAGAGCTGAT GTTCGAAAAG TAGCACATCG ATTCCTGGTG
    GAAAACTTGA ATCCTTTAAT ATTAAGTTAT GTTAAAAAGA AGAGGTTCCA TCAAATATTT
    CTTGAGGAGA TGCCCTGGAA AGCTCAGATG AATCTGTATC TGGCAAGCGC ACACTTCAAC
    TTGCTTTTAA CAAAGCTAGG GGAACGTACA AAGATAAAAT TTGGTTCTTC ACCGATAATG
    GTCAGTTTCC GATCATGTGA CCCTACTATG TTCTCACTGT ATAATTCAGG AACAGTATTA
    CCAACAGGAA GAATGACTCT AGAAAACTAT AAAGTGATGC TTGATTTCCT TCTTACAGCT
    AAAAAAAGAA AGGCTCACTT ACCATCAGAT GCTGAAGAAT TTTTGGCATT TCTTAATTCC
    AAAATGAGTG GTGAAAATGT TTCCAAGACC CAAACGGTTT ATGAATCAGA CTCTCAGTCG
    GGACTTAGCA GTAAAGAAAA GGATCGATCT GCAAATGTGG GTCTCTTGGA TCATTTTATG
    AAAATCTTTT TATATTGCAG GAGAGCAATG GTTCTTGCTC ATCGTGGTGG CTATTGGACT
    CTGCTTCAGA ACTGCTGTCG GGCCCTTTGG AACTTTACTC AGGAGCTACA AATACTGCAT
    AAACAAGCAG TGGATCTGTA TAAGACATTT CCTATTAGCC AGGATAGTTT CCTCTGTATC
    TCTGTTTTAC CATTCTATTT GGGAGCAGAA TTACTTATTG ACATGTTAAT AGAACTACAG
    AATACTGATT CTATTAAGAC TATTGATGAA AAAGGAGAGT TCAGTGTCCC GAGTTGCTAT
    GGAAATATTA AAAATGATAA CGGTGGTTCC AGCCTTACCT TTGAGCATCC TCTGGATGAC
    GTCAATGTGG TCGATTTGAA ATGGATCCAT GACTTTGTAT TAAAATCTCT GGAACTTGTC
    TATCAAGTAG AAAAATGGGA AACACTCGTA TCACTTGCCA TTCAGTTCAA TGCAGTTTCG
    CATGAAAGAT ACACAGAGCA AGTGACTCCA CTGCTGGTTT ACGCACAGCG CCAGCTTCTA
    CTGCAAATAT CCAAATGCAA GGGCCCAGAC ATCAGCCAAC AGGCTTGTGT AAGATATGAG
    ACTGAATACA AACAGAAGAT AACCTGCCGA AATTTCATTG GAATACAGCT CAAGATTAAT
    CCTCCAACCA ATAAAGGGAC AGCTATAGGA CAGCGAGCAG ATGTCCTTAG AAAGCTTATC
    TATTCAGAAT ACAGCAGAGC CAAAGAGCTC CTCTGTGTGC CTGTGGACGT GACAGACACT
    CTGAGATGTT TTAGAGAGAC CTTGGAAAAA TCCAAATATC ACAACAGATC AATCCGGCAC
    AGCAGAAAGT TGCTTTCGTT ATTTCTCGCA CAGACACAAG GAGACAAAGG AGGAGTGAAC
    AATTTAAAAT TTGCTACTGG GAAGGTGGAG TTTTCCATGG GGACAGAAGA GATGCACATG
    CCAACACCCC CCGACCTTTC TCAGGAGCAC TTCCGAGTTT TCAGCTCAGT GGAGAAGAGC
    AAGCTTCCCT CCTCACAACT TGGACTCGTA ATTTCTTCTT ATTATGAAAC TATTAATGTT
    CTTCAAACCA GCAATCAAAG AAGTCTTCAT GTGCAGGCGT TGCATGAACT TGGAAATCTT
    CTCATCTTCG CACAGAAGAA AAGGGCTGCT TTTAAGTGTT GGAGTCAAGC TCTGGATGAA
    ATTTTCAGAA AAGAAGATGT GCTCCATACA TGGAAGGAGA TTGGCAGCTC ACTCACCAGT
    GCCACTGATG GCTGGTCACC TCCAGGTTCC AAAGACTACA GTGAGGAGCT CCTGTCCAAA
    GGCGGCATCT GGGGGTGTTT GCAGGGAGCG GTGATATCAG CAAAGATAGC ACAATTTATT
    CAGACATTGG ATGTTGAAAA GAGAACCAAT TGTTGCCTTT TATCAGCATT ACTCTTTCAG
    GGTTTGCTTA GAACCACACT GCCGCATCCT AAAGCTGAAC GTTGCTACGC TCAGTATGAA
    ATCACTCAGC TTCTCCCGGG CATCGAGCTC TTCTCAGACA GATATCGGGC TGACATCTGC
    TCCGTCATTG CGAGTTTATA TTACGTCATC CGCGAGCTGC ACTTTGCTAA GCAAAATCTA
    GTAATTCTGC CTCTCCTTGC ATTGTACCAA TATTTTGTTT CTGTAATTTG CCAAGACCTA
    GTCAAAAATC TGGAAGCAAG AATCCTCAAG ATAGAAGTCC TTACAGATTT AGGATTCTTT
    TCCGAAGCCT TTCATGAGCT ATCCCAGATA TTTTATGCGA AAAACACACC TTCTTCAGTA
    CCCACCAGCT GTAAACCTAC TGGAAAAATG AAGCTATTTC AATCATTTGA CTCGGGAAAA
    CCTCTTATTA GTAAAGAGAA TCTGCAGGCA CTTGATGATT TAATAAATAA AGGAATGCCT
    GTAATTCTGA CTACAGTCAG CCAGCAACAT CTCTTAAATA AGTTTTTAGT TGTTAAAGCA
    TACTTTTTCA TAAATGTGGC TGCAACAATA AATTGTGTCC CAGAACAGAC AATGAAACCA
    GTCGGCCATG GAGTTATGAA TGAGAAAAGC AAACCAAATC TTCCAAACTT GAAAGAGATG
    TGTTTAAAGG ATGAGGGAGG CTCACTTTGT CATCTGACAA AATTGAAAGA GAACTTTGCT
    CTTAGCACAC TAAAGTCAAT TTTACTGACA GAAGCTGAAG ACAGACTCAA CACCCTTCTG
    TCGGAGATGG ACCATCTAAG CCATAAGGAC CTCTCTCAGT GTTCTGCTGG CGAACTGGAG
    ATTGTAGTGG AGGTCAGACT GCAGCTGGCT GCCATTGCCC TGCAGAGACA CCGGGCAGCA
    TACAGTGCTG CGATAGTATT TTCCACACTT AAACTTCTCC AGGATTCAAA ACTTTTTAAA
    AAGAAAGTAG TAGAAGATGA CTCAGAGAAT TGCACCTCTT CAGAAAGTTA TGCGACTGAA
    AGTAAAGACG ACAATTTTTT AGATCCTATT TCCTTAAATT CTCGAGAATA TTTCAACATT
    CATCTGTGGT TGAGGTGCCG CTTAGCCTTG GTGACTGCAT TTGTTGCCCA GATTCGTGGC
    ATTGGAATCG TGAAAGAAAA CGATATAACA GATTACGTGA GCCTTATCAA TGAAGTGTGT
    GTGGAGGCAA AAGCTGCAGG TGACAGGGAG CTACAGGCTG AATTCTTGAT GCAAGCTGTA
    ATTATCGGCC TACAAGAAAA GCATTTAAAG GCAGACATCA TAAAAAGCCT TCAGGAGGTA
    ATACAGTTGC TTGAAGGGCA TGACTTTATT TCTCCTCGAT CTCATTTAAC CCTGGTGAGA
    AGCATGCTTT TGCTGGATGA TTTAACAAAA GCTGAGAAAT TCAAGGAAAC TCCTTTTTCG
    AAAATAGAAA AATTGTATTT GTTGACTCAG TCTCACAACA TTCTTATAGA ACAGATGATA
    ACTTTTGGAG AAACAATTGA ATTGCCTTCA TCAAACACTG ACTATGCAAG TCCATTACTG
    CCTTTGAAAA ATATTTATCT TCCTCACGTC ATGTTGTTGG CCAAAACAAA AATGAGAATT
    GGACATACAG TGGCCAAGCA AGTATATTAT ACCAGTAAAA AGAAGGATTC TTCAAAGTGG
    TTACCTGCCC TTCATTTTTT TGAAATAGCT TTGAAGCTCG CTAGGACATC AGGAACAGAA
    GAACATGAGG TGGAAGCTGA AATCTTTTTT CAGAAAGGCA AAATAGAATG TCAAATATTG
    ATGGAAGAGA AACCTCCAAC TATGCAACTT GAGAGTCTGT TTGAAGCTAT ACAGCTAAGC
    TTGAGAAATG ATCAAAATTC AGGGTTGATA AGAGACTCCT ACCTTGAAAT AGCCTTATTA
    TTTTTACACA TGAGGAAGTT AAAGAGCAAA CTTTCAGTGT CACCATTGAT GAGTATTAAG
    GCTCTTCCCA GAAGGCATAG TTCTATTAAA GAACCAGTAG TAAATAAACA TGAAATGTAC
    AGTTTATTTG CCTGGATTGC AATAAGAGCT GCTGCACAGG TCAGTGAGGC TGTGCTGGCA
    ATTAATCTAC TTATTGGAAA GAAGAATGCT AGAACTGATA CAGTTAGCCA AGGGGCATTA
    TCAAATATTC CAGAATTTGC TTCCATGGAT CTTTTGTCTT CATACACAGA TTATTTGCTT
    GATAACTACC AAGTTGTCTT CCAGACTGGT TGTACATTTT CTTACGTTAG TGAAGAGATA
    CATGAAGATG TAGATTCTCA GAAAAAAAAC CCAATTAAAG TGGATATTAC ATGGATTCTT
    CTGCTGCGCT ACTATATTCA CCTACAAAGA ATTAATAATA TGAGCAAATT GCTAGCATCT
    CTGAAGCCAG GATCTGGGAT GTCTTTGCCA GATGACACTC TTCTCACTTC TCTTTTTAAC
    TCTGGATTGA TTTTGCGCCA AAAAGAAATG CATTTTTTCC TTAAAAGATT CTTACAGCTA
    TATTCTTCTT CTTGTATTGA TGAATTTCCA AAAGAACTAT TTCAAGTCAT GGAACATCCA
    CCTGTTCTAG AAAAAGTCTT ATATGATTCA GCAAGCAAGA CAGGAGTAAC CAATGCTTCT
    CTATGCTCCG ATTTATCACT AAAGACCGTT AGCAGCACAT TGTATGGAGA CGTGGTGCCA
    CCAGTTGTGG CAACACAGGC TTTAAACAAA GAACTTTGTT TTCAGTGGTA CATTCCTCCC
    CTGGAAAAAC CTCCAAAGGA AACAGAACCT ATGGTTTTAT TGTTGTATGC ATATAATATG
    AAGCCCCTGA ATATTTCTGA TGTTGCATCT GCTGCTTGCA ACTGTGTGTA CGTAGGCTCT
    TTCTGGGTTC CACTAAAAAG AGTTATTTCA GTTCATGAGA AACTATCTAA TCTTACGCAA
    ATTGCTGAAC TATCATTGCC AACAACTCCT AAAGTTACTT CAAATGAAAA TTTACATGAA
    GTAGAGGAAA CAGAAGAGAA ATCAATTGAT GCAGAAATGG AAAACATGAT TATTGTCTGT
    TGCAATGAAA TAGTATCTTT GTTTTTGGGT GATGAAATGG CAACTCCACT ATCTGAGGTC
    CCATTTGATG TCTCGCTGCC ATCTATATTC AGTCTGGAGA GACTTTTTGA TCTTGCTAGT
    GGCTGTATCG TATCGGGAGG AAGCCTTTTC AACTGGGTGG TATCAATTAT TCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848280.1
    CDS414..9230
    Translation

    Target ORF information:

    RefSeq Version XM_024992512.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992512.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    ATGCAGGTGG CTTTGCCACA GGAGCACCTG TGCTGGATTA TCTTCAATGG TACCATTTAC 
    ATTTACACTA TTTGCAGAAA ATTGATGATC AGAGGTCATT CTTCCAAGGC CTTGGAATAC
    CTCCTGTGGG CCAGTATATG TATGGAGTCT TCAGTGCCTC TCCTGTCCAT CAGGTACTTG
    ACGTGGAGAG CTACTCTTTA CACAGCTGTC TGCCAGTGCT ACTATGACTG CCAAGCTGGT
    GTTCATGGAG AGGCATTTGC TCGGCGTGCT TTAGCTAAAA TTGATGAGTT ACGGCAACTG
    GAATTAATGA GTTCCTCACA ACCACAGGAA GAATCCAGAG AATATTATAG AGAGGCCACA
    ATAAAGATGG CAGTCATGAT CTTCAAAAGA GGAGTATTTG AATCTAGAAG AAAAAACAAA
    AGTTTCTTTA GACCAAAGAT AAGAGTTAAC ATAAAGGAAG CACAAACTAT GCCATGGCCG
    CGTACAGCCA CAGAAAAGCT GTTGGAGGAG TTGTTTGATA GCACTGCCTC CCGGTTTCTG
    GCTGTCTTGG AAGCGCTCTC TGACTCAAAT CGGCGAATAC TACAGACTGG ACCTGTTTTT
    ACAGATGAAG TAGAAGTTCG TGATGTTGTC TCAGAACTGT TTATGGCAGG AAAAGAACTT
    TTAATAATGT CAAATATTGG TGCAGATGGC ATACTTGATT TTCCAAAAGC ACATCTTTTG
    GAACTTATTA TAAAAAAGAA GAATCTTATT TCTGTGAATG CTGCCGTGAA ATTTATAAAA
    TTAGCTTTCA TGTATGAGGA ATGGAGTTTA TTTGAATCTG CGGCTGGGCA GCTCATTGAT
    TTTCTCCAGA GGCAGGATGA CCCAGCGTCA AAGAAAGCGG AAAAGAATTT AATTCTTCTT
    GTTGCGATAG AACCTTTGAT CAATGTAAAG AGAAACAAAG GTTTGATCTT TCCTTTTGAC
    AACTACAAAG AAGGTCAAGT CTATGTAAAA AAAATTGCTG TTCATGATAC TTATGTGAAG
    TCTTGTGGAT ACTCAGAAGA CATTTTCCAT TTGGCGACAA CCTTGTATTC CTGTGTCTGT
    GACTCTCCAC AGAATGCTCA GCCGGATAAG GAAATCGTTG TGGACATGAT AATGTTCCTG
    TGGCAGAAAT GCAAATTAGG AATGCAGCGG GTCACTGTGT CCAAAAATGA CTACTCAAAG
    TTTACCCAGA AGATCAGTAC TAATAAATGG CTTTATCTTC TGTGGCAGAT AAATGAAATC
    ATTCACTGCT ATAAGATGGA AGACATTGAT GTTGTGATGG TGGCAGAGAT CACGTTACGA
    TTAAGTGAAA TATTGGAATC TTTAGGAAAC CCAAGAAGGA AATTTAAAAA ATTTCTAGAT
    GTATCTTTAA AGAGAGGGAC TAGTGAAATC TTTGGGGGTT CGAAAGGGAG CATAGATATT
    CTTCCAATAA TAAAGAAAAA ACCTGAAGAA CAGTTATTTA TGGCCTATGA ACTTCTTGAC
    AAAGCCATTG ATGGCATAAA TTTGAACTGC ATGTTAACTG CTTTGCCAAA TGGATCATCA
    GTGATTGACC ATTGCTATGC CACGCACAAC CACTATATAG ATGGAGACAT GTGCAAACCA
    GTCACACCGA ATAATTTCAT AATGGATTTG CATCTTGAAC TAATTCAAGC CCAGCACCGA
    ATAGCTGTTG TGCTTCTTGA CCAGTTGCAA GTTTTGCAGA CTCCAACTGT GAGCAAAAAT
    ACATCTACCA AAGGTACAGA GAAGCTAAAA AAACCTGCAA GCCCTGATTG TTTTACAGAA
    TTAAGTGTGA TGAATAAAAT AAGGAAGAAT AAGCTATCTA AAGCGATTTA TTTGATGCAG
    AAAACTCTTC TAATATTTGA GAAAGATGCA ACCTGTTCTT CTCTACAGAA CTACTTGACG
    GAAGCATATT CTTTGATTGA GAAGACCGAA TCAGAACAAA GTATACAATA TTCATATCAG
    AAATATGTGG AAACTGCCAA AAGAAAGAAA AGCAGGGTCC CTCCTCCACC CATCCTGCTA
    TCCCGAACTT ACCGTTCTGT GACATTAAAA CCTGCCCCAT TTGTTTCTGA TGTTAAGGTC
    TGCTGGTACT GCATTTTGGG TCGCAAAGCA GAAGGAAGCT ATGGAAAAGT AAGACTAAAC
    GATAATCATC TCCCAAACTC AGGAGAAGCG ATACCAGCAG ATGGCAAAAG CGTTTTTGAG
    GTAAAAGGTT TAGAAACCAA TGAAAAATAC GTCTTTGCAG TTGCGGCTTA CTGTTCTAAA
    GGAAAGCTGA TTGGTGATGC TATTGGCGAG ACAACTAAAC CAATTCTGGT TTACCCACCT
    CTTTCTGCTG TTACTACTCG GATGTTATTG ACGCAGGTCG CCTATCAGAC TGGTAACTAT
    GATCTGGCTA AGAAAGTTTT CTCACCAGTC TGGGATTATT TTGTTGCTTC ACCACCTCAG
    GGTGACCAAT CCATTATTTG TTTAAGCAAT ATAATGACTA TTACACAGAG AAGATTACAT
    TCAGATATTT TGGCAGAGAG TTCTTCAATT CTCTTATATC TTTTTCTTAG AAATATTTTT
    GTAACAAGTG ACATTAAAAT TAAAGAAGAA AATTTTTTCT GTGATAATAT TAAAGGCAAT
    GAGATTTTCC CTTCTCAACA AATTGCTAGA CTAATTGAAT GTGAGAGAGT ATTGGTGGCA
    TTGGAACTGA GCAACTACCT AAATGATTCC AGTTATGCCC TTCAGGCTGT GACTCAGTGT
    TACGGCCTCC TTGCTCCTAT CATTTATCAC AATATTGTTT TGGTACCTGT TGTACAGATC
    TTGATAAAGT GTGTGGTAGT TTTGCAAGGA CTACCAAATG TCATCCACTC AAAGAAACAT
    CTTGCAAGCT TTGAAAGTGT ACAGCATATG ATAGCTTGCT GTATCTTCTA CATAACAAAG
    ATCCTGCGTT CATGGAAGGA ATATGACCTG GCAGTAATGA TTATAAATTA TGGAAAAAAG
    GTGTTGGACA TCTCATCAGG TTGCAAATCT CTATTTGGTA CTACAGACCT AGAGGAAATG
    CAAGAAGAGG GTTCTTCCAA GAAGTCTTGC AAGAATAAGA AGCCACAGCA GGTATTAGTA
    CCTGAAAAAA TAAATGAACA GCTGGTTTTA TTGGAGACAC ATCTACTCAA ACTGACAAAG
    CAATATATTA CAACTGAACT CTCAGGAGCA GAAGATCCCA TATTTCTTTA CCCTATAGTT
    TTAAACTGGT CGGTCAAAGG TGCTGTGAAA GAAGTTATGA AATTTAAGCA GAGACCTAGA
    TTCCTGGAAT TCTTTACACA AGTTATGCAA AAATGTATGC ATGATGAAAA ATTTCAACCT
    ATGGTGGAGA TAACAAATCC TGTCTATGAC TTCTTGAAAA GGAGGAATGA GTCCCTTCTT
    GGAATAAAAA AACTGAAATA CAAGGATACT TTGTCTAAAA AGGTCATCAA ACCTGTGAAG
    AAGTACAGAG CTGCAGTAGT GGAGATTGGA AAAACTACAG AAATGAAGCA AAAAAAAAAA
    GCTAAAAGAA AGGAAACTCT AAGAGAATTT TTGAATAAAA ATCCCTTTAT TTCTGAAATG
    CCGGAGCATG AAAGAAATAA GAGAGCTGAT GTTCGAAAAG TAGCACATCG ATTCCTGGTG
    GAAAACTTGA ATCCTTTAAT ATTAAGTTAT GTTAAAAAGA AGAGGTTCCA TCAAATATTT
    CTTGAGGAGA TGCCCTGGAA AGCTCAGATG AATCTGTATC TGGCAAGCGC ACACTTCAAC
    TTGCTTTTAA CAAAGCTAGG GGAACGTACA AAGATAAAAT TTGGTTCTTC ACCGATAATG
    GTCAGTTTCC GATCATGTGA CCCTACTATG TTCTCACTGT ATAATTCAGG AACAGTATTA
    CCAACAGGAA GAATGACTCT AGAAAACTAT AAAGTGATGC TTGATTTCCT TCTTACAGCT
    AAAAAAAGAA AGGCTCACTT ACCATCAGAT GCTGAAGAAT TTTTGGCATT TCTTAATTCC
    AAAATGAGTG GTGAAAATGT TTCCAAGACC CAAACGGTTT ATGAATCAGA CTCTCAGTCG
    GGACTTAGCA GTAAAGAAAA GGATCGATCT GCAAATGTGG GTCTCTTGGA TCATTTTATG
    AAAATCTTTT TATATTGCAG GAGAGCAATG GTTCTTGCTC ATCGTGGTGG CTATTGGACT
    CTGCTTCAGA ACTGCTGTCG GGCCCTTTGG AACTTTACTC AGGAGCTACA AATACTGCAT
    AAACAAGCAG TGGATCTGTA TAAGACATTT CCTATTAGCC AGGATAGTTT CCTCTGTATC
    TCTGTTTTAC CATTCTATTT GGGAGCAGAA TTACTTATTG ACATGTTAAT AGAACTACAG
    AATACTGATT CTATTAAGAC TATTGATGAA AAAGGAGAGT TCAGTGTCCC GAGTTGCTAT
    GGAAATATTA AAAATGATAA CGGTGGTTCC AGCCTTACCT TTGAGCATCC TCTGGATGAC
    GTCAATGTGG TCGATTTGAA ATGGATCCAT GACTTTGTAT TAAAATCTCT GGAACTTGTC
    TATCAAGTAG AAAAATGGGA AACACTCGTA TCACTTGCCA TTCAGTTCAA TGCAGTTTCG
    CATGAAAGAT ACACAGAGCA AGTGACTCCA CTGCTGGTTT ACGCACAGCG CCAGCTTCTA
    CTGCAAATAT CCAAATGCAA GGGCCCAGAC ATCAGCCAAC AGGCTTGTGT AAGATATGAG
    ACTGAATACA AACAGAAGAT AACCTGCCGA AATTTCATTG GAATACAGCT CAAGATTAAT
    CCTCCAACCA ATAAAGGGAC AGCTATAGGA CAGCGAGCAG ATGTCCTTAG AAAGCTTATC
    TATTCAGAAT ACAGCAGAGC CAAAGAGCTC CTCTGTGTGC CTGTGGACGT GACAGACACT
    CTGAGATGTT TTAGAGAGAC CTTGGAAAAA TCCAAATATC ACAACAGATC AATCCGGCAC
    AGCAGAAAGT TGCTTTCGTT ATTTCTCGCA CAGACACAAG GAGACAAAGG AGGAGTGAAC
    AATTTAAAAT TTGCTACTGG GAAGGTGGAG TTTTCCATGG GGACAGAAGA GATGCACATG
    CCAACACCCC CCGACCTTTC TCAGGAGCAC TTCCGAGTTT TCAGCTCAGT GGAGAAGAGC
    AAGCTTCCCT CCTCACAACT TGGACTCGTA ATTTCTTCTT ATTATGAAAC TATTAATGTT
    CTTCAAACCA GCAATCAAAG AAGTCTTCAT GTGCAGGCGT TGCATGAACT TGGAAATCTT
    CTCATCTTCG CACAGAAGAA AAGGGCTGCT TTTAAGTGTT GGAGTCAAGC TCTGGATGAA
    ATTTTCAGAA AAGAAGATGT GCTCCATACA TGGAAGGAGA TTGGCAGCTC ACTCACCAGT
    GCCACTGATG GCTGGTCACC TCCAGGTTCC AAAGACTACA GTGAGGAGCT CCTGTCCAAA
    GGCGGCATCT GGGGGTGTTT GCAGGGAGCG GTGATATCAG CAAAGATAGC ACAATTTATT
    CAGACATTGG ATGTTGAAAA GAGAACCAAT TGTTGCCTTT TATCAGCATT ACTCTTTCAG
    GGTTTGCTTA GAACCACACT GCCGCATCCT AAAGCTGAAC GTTGCTACGC TCAGTATGAA
    ATCACTCAGC TTCTCCCGGG CATCGAGCTC TTCTCAGACA GATATCGGGC TGACATCTGC
    TCCGTCATTG CGAGTTTATA TTACGTCATC CGCGAGCTGC ACTTTGCTAA GCAAAATCTA
    GTAATTCTGC CTCTCCTTGC ATTGTACCAA TATTTTGTTT CTGTAATTTG CCAAGACCTA
    GTCAAAAATC TGGAAGCAAG AATCCTCAAG ATAGAAGTCC TTACAGATTT AGGATTCTTT
    TCCGAAGCCT TTCATGAGCT ATCCCAGATA TTTTATGCGA AAAACACACC TTCTTCAGTA
    CCCACCAGCT GTAAACCTAC TGGAAAAATG AAGCTATTTC AATCATTTGA CTCGGGAAAA
    CCTCTTATTA GTAAAGAGAA TCTGCAGGCA CTTGATGATT TAATAAATAA AGGAATGCCT
    GTAATTCTGA CTACAGTCAG CCAGCAACAT CTCTTAAATA AGTTTTTAGT TGTTAAAGCA
    TACTTTTTCA TAAATGTGGC TGCAACAATA AATTGTGTCC CAGAACAGAC AATGAAACCA
    GTCGGCCATG GAGTTATGAA TGAGAAAAGC AAACCAAATC TTCCAAACTT GAAAGAGATG
    TGTTTAAAGG ATGAGGGAGG CTCACTTTGT CATCTGACAA AATTGAAAGA GAACTTTGCT
    CTTAGCACAC TAAAGTCAAT TTTACTGACA GAAGCTGAAG ACAGACTCAA CACCCTTCTG
    TCGGAGATGG ACCATCTAAG CCATAAGGAC CTCTCTCAGT GTTCTGCTGG CGAACTGGAG
    ATTGTAGTGG AGGTCAGACT GCAGCTGGCT GCCATTGCCC TGCAGAGACA CCGGGCAGCA
    TACAGTGCTG CGATAGTATT TTCCACACTT AAACTTCTCC AGGATTCAAA ACTTTTTAAA
    AAGAAAGTAG TAGAAGATGA CTCAGAGAAT TGCACCTCTT CAGAAAGTTA TGCGACTGAA
    AGTAAAGACG ACAATTTTTT AGATCCTATT TCCTTAAATT CTCGAGAATA TTTCAACATT
    CATCTGTGGT TGAGGTGCCG CTTAGCCTTG GTGACTGCAT TTGTTGCCCA GATTCGTGGC
    ATTGGAATCG TGAAAGAAAA CGATATAACA GATTACGTGA GCCTTATCAA TGAAGTGTGT
    GTGGAGGCAA AAGCTGCAGG TGACAGGGAG CTACAGGCTG AATTCTTGAT GCAAGCTGTA
    ATTATCGGCC TACAAGAAAA GCATTTAAAG GCAGACATCA TAAAAAGCCT TCAGGAGGTA
    ATACAGTTGC TTGAAGGGCA TGACTTTATT TCTCCTCGAT CTCATTTAAC CCTGGTGAGA
    AGCATGCTTT TGCTGGATGA TTTAACAAAA GCTGAGAAAT TCAAGGAAAC TCCTTTTTCG
    AAAATAGAAA AATTGTATTT GTTGACTCAG TCTCACAACA TTCTTATAGA ACAGATGATA
    ACTTTTGGAG AAACAATTGA ATTGCCTTCA TCAAACACTG ACTATGCAAG TCCATTACTG
    CCTTTGAAAA ATATTTATCT TCCTCACGTC ATGTTGTTGG CCAAAACAAA AATGAGAATT
    GGACATACAG TGGCCAAGCA AGTATATTAT ACCAGTAAAA AGAAGGATTC TTCAAAGTGG
    TTACCTGCCC TTCATTTTTT TGAAATAGCT TTGAAGCTCG CTAGGACATC AGGAACAGAA
    GAACATGAGG TGGAAGCTGA AATCTTTTTT CAGAAAGGCA AAATAGAATG TCAAATATTG
    ATGGAAGAGA AACCTCCAAC TATGCAACTT GAGAGTCTGT TTGAAGCTAT ACAGCTAAGC
    TTGAGAAATG ATCAAAATTC AGGGTTGATA AGAGACTCCT ACCTTGAAAT AGCCTTATTA
    TTTTTACACA TGAGGAAGTT AAAGAGCAAA CTTTCAGTGT CACCATTGAT GAGTATTAAG
    GCTCTTCCCA GAAGGCATAG TTCTATTAAA GAACCAGTAG TAAATAAACA TGAAATGTAC
    AGTTTATTTG CCTGGATTGC AATAAGAGCT GCTGCACAGG TCAGTGAGGC TGTGCTGGCA
    ATTAATCTAC TTATTGGAAA GAAGAATGCT AGAACTGATA CAGTTAGCCA AGGGGCATTA
    TCAAATATTC CAGAATTTGC TTCCATGGAT CTTTTGTCTT CATACACAGA TTATTTGCTT
    GATAACTACC AAGTTGTCTT CCAGACTGGT TGTACATTTT CTTACGTTAG TGAAGAGATA
    CATGAAGATG TAGATTCTCA GAAAAAAAAC CCAATTAAAG TGGATATTAC ATGGATTCTT
    CTGCTGCGCT ACTATATTCA CCTACAAAGA ATTAATAATA TGAGCAAATT GCTAGCATCT
    CTGAAGCCAG GATCTGGGAT GTCTTTGCCA GATGACACTC TTCTCACTTC TCTTTTTAAC
    TCTGGATTGA TTTTGCGCCA AAAAGAAATG CATTTTTTCC TTAAAAGATT CTTACAGCTA
    TATTCTTCTT CTTGTATTGA TGAATTTCCA AAAGAACTAT TTCAAGTCAT GGAACATCCA
    CCTGTTCTAG AAAAAGTCTT ATATGATTCA GCAAGCAAGA CAGGAGTAAC CAATGCTTCT
    CTATGCTCCG ATTTATCACT AAAGACCGTT AGCAGCACAT TGTATGGAGA CGTGGTGCCA
    CCAGTTGTGG CAACACAGGC TTTAAACAAA GAACTTTGTT TTCAGTGGTA CATTCCTCCC
    CTGGAAAAAC CTCCAAAGGA AACAGAACCT ATGGTTTTAT TGTTGTATGC ATATAATATG
    AAGCCCCTGA ATATTTCTGA TGTTGCATCT GCTGCTTGCA ACTGTGTGTA CGTAGGCTCT
    TTCTGGGTTC CACTAAAAAG AGTTATTTCA GTTCATGAGA AACTATCTAA TCTTACGCAA
    ATTGCTGAAC TATCATTGCC AACAACTCCT AAAGTTACTT CAAATGAAAA TTTACATGAA
    GTAGAGGAAA CAGAAGAGAA ATCAATTGAT GCAGAAATGG AAAACATGAT TATTGTCTGT
    TGCAATGAAA TAGTATCTTT GTTTTTGGGT GATGAAATGG CAACTCCACT ATCTGAGGTC
    CCATTTGATG TCTCGCTGCC ATCTATATTC AGTCTGGAGA GACTTTTTGA TCTTGCTAGT
    GGCTGTATCG TATCGGGAGG AAGCCTTTTC AACTGGGTGG TATCAATTAT TCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234805
    Clone ID Related Accession (Same CDS sequence) XM_024992513.1
    Accession Version XM_024992513.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2581
    2641
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    2941
    3001
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    3181
    3241
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    3421
    3481
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    4141
    4201
    4261
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    4381
    4441
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    4681
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    5821
    5881
    5941
    6001
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    6181
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    6301
    6361
    6421
    6481
    6541
    6601
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    6781
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    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GGAGACAAAG GAGGAGTGAA CAATTTAAAA
    TTTGCTACTG GGAAGGTGGA GTTTTCCATG GGGACAGAAG AGATGCACAT GCCAACACCC
    CCCGACCTTT CTCAGGAGCA CTTCCGAGTT TTCAGCTCAG TGGAGAAGAG CAAGCTTCCC
    TCCTCACAAC TTGGACTCGT AATTTCTTCT TATTATGAAA CTATTAATGT TCTTCAAACC
    AGCAATCAAA GAAGTCTTCA TGTGCAGGCG TTGCATGAAC TTGGAAATCT TCTCATCTTC
    GCACAGAAGA AAAGGGCTGC TTTTAAGTGT TGGAGTCAAG CTCTGGATGA AATTTTCAGA
    AAAGAAGATG TGCTCCATAC ATGGAAGGAG ATTGGCAGCT CACTCACCAG TGCCACTGAT
    GGCTGGTCAC CTCCAGGTTC CAAAGACTAC AGTGAGGAGC TCCTGTCCAA AGGCGGCATC
    TGGGGGTGTT TGCAGGGAGC GGTGATATCA GCAAAGATAG CACAATTTAT TCAGACATTG
    GATGTTGAAA AGAGAACCAA TTGTTGCCTT TTATCAGCAT TACTCTTTCA GGGTTTGCTT
    AGAACCACAC TGCCGCATCC TAAAGCTGAA CGTTGCTACG CTCAGTATGA AATCACTCAG
    CTTCTCCCGG GCATCGAGCT CTTCTCAGAC AGATATCGGG CTGACATCTG CTCCGTCATT
    GCGAGTTTAT ATTACGTCAT CCGCGAGCTG CACTTTGCTA AGCAAAATCT AGTAATTCTG
    CCTCTCCTTG CATTGTACCA ATATTTTGTT TCTGTAATTT GCCAAGACCT AGTCAAAAAT
    CTGGAAGCAA GAATCCTCAA GATAGAAGTC CTTACAGATT TAGGATTCTT TTCCGAAGCC
    TTTCATGAGC TATCCCAGAT ATTTTATGCG AAAAACACAC CTTCTTCAGT ACCCACCAGC
    TGTAAACCTA CTGGAAAAAT GAAGCTATTT CAATCATTTG ACTCGGGAAA ACCTCTTATT
    AGTAAAGAGA ATCTGCAGGC ACTTGATGAT TTAATAAATA AAGGAATGCC TGTAATTCTG
    ACTACAGTCA GCCAGCAACA TCTCTTAAAT AAGTTTTTAG TTGTTAAAGC ATACTTTTTC
    ATAAATGTGG CTGCAACAAT AAATTGTGTC CCAGAACAGA CAATGAAACC AGTCGGCCAT
    GGAGTTATGA ATGAGAAAAG CAAACCAAAT CTTCCAAACT TGAAAGAGAT GTGTTTAAAG
    GATGAGGGAG GCTCACTTTG TCATCTGACA AAATTGAAAG AGAACTTTGC TCTTAGCACA
    CTAAAGTCAA TTTTACTGAC AGAAGCTGAA GACAGACTCA ACACCCTTCT GTCGGAGATG
    GACCATCTAA GCCATAAGGA CCTCTCTCAG TGTTCTGCTG GCGAACTGGA GATTGTAGTG
    GAGGTCAGAC TGCAGCTGGC TGCCATTGCC CTGCAGAGAC ACCGGGCAGC ATACAGTGCT
    GCGATAGTAT TTTCCACACT TAAACTTCTC CAGGATTCAA AACTTTTTAA AAAGAAAGTA
    GTAGAAGATG ACTCAGAGAA TTGCACCTCT TCAGAAAGTT ATGCGACTGA AAGTAAAGAC
    GACAATTTTT TAGATCCTAT TTCCTTAAAT TCTCGAGAAT ATTTCAACAT TCATCTGTGG
    TTGAGGTGCC GCTTAGCCTT GGTGACTGCA TTTGTTGCCC AGATTCGTGG CATTGGAATC
    GTGAAAGAAA ACGATATAAC AGATTACGTG AGCCTTATCA ATGAAGTGTG TGTGGAGGCA
    AAAGCTGCAG GTGACAGGGA GCTACAGGCT GAATTCTTGA TGCAAGCTGT AATTATCGGC
    CTACAAGAAA AGCATTTAAA GGCAGACATC ATAAAAAGCC TTCAGGAGGT AATACAGTTG
    CTTGAAGGGC ATGACTTTAT TTCTCCTCGA TCTCATTTAA CCCTGGTGAG AAGCATGCTT
    TTGCTGGATG ATTTAACAAA AGCTGAGAAA TTCAAGGAAA CTCCTTTTTC GAAAATAGAA
    AAATTGTATT TGTTGACTCA GTCTCACAAC ATTCTTATAG AACAGATGAT AACTTTTGGA
    GAAACAATTG AATTGCCTTC ATCAAACACT GACTATGCAA GTCCATTACT GCCTTTGAAA
    AATATTTATC TTCCTCACGT CATGTTGTTG GCCAAAACAA AAATGAGAAT TGGACATACA
    GTGGCCAAGC AAGTATATTA TACCAGTAAA AAGAAGGATT CTTCAAAGTG GTTACCTGCC
    CTTCATTTTT TTGAAATAGC TTTGAAGCTC GCTAGGACAT CAGGAACAGA AGAACATGAG
    GTGGAAGCTG AAATCTTTTT TCAGAAAGGC AAAATAGAAT GTCAAATATT GATGGAAGAG
    AAACCTCCAA CTATGCAACT TGAGAGTCTG TTTGAAGCTA TACAGCTAAG CTTGAGAAAT
    GATCAAAATT CAGGGTTGAT AAGAGACTCC TACCTTGAAA TAGCCTTATT ATTTTTACAC
    ATGAGGAAGT TAAAGAGCAA ACTTTCAGTG TCACCATTGA TGAGTATTAA GGCTCTTCCC
    AGAAGGCATA GTTCTATTAA AGAACCAGTA GTAAATAAAC ATGAAATGTA CAGTTTATTT
    GCCTGGATTG CAATAAGAGC TGCTGCACAG GTCAGTGAGG CTGTGCTGGC AATTAATCTA
    CTTATTGGAA AGAAGAATGC TAGAACTGAT ACAGTTAGCC AAGGGGCATT ATCAAATATT
    CCAGAATTTG CTTCCATGGA TCTTTTGTCT TCATACACAG ATTATTTGCT TGATAACTAC
    CAAGTTGTCT TCCAGACTGG TTGTACATTT TCTTACGTTA GTGAAGAGAT ACATGAAGAT
    GTAGATTCTC AGAAAAAAAA CCCAATTAAA GTGGATATTA CATGGATTCT TCTGCTGCGC
    TACTATATTC ACCTACAAAG AATTAATAAT ATGAGCAAAT TGCTAGGTTT TATTGTTGTA
    TGCATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848281.1
    CDS932..9700
    Translation

    Target ORF information:

    RefSeq Version XM_024992513.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992513.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GGAGACAAAG GAGGAGTGAA CAATTTAAAA
    TTTGCTACTG GGAAGGTGGA GTTTTCCATG GGGACAGAAG AGATGCACAT GCCAACACCC
    CCCGACCTTT CTCAGGAGCA CTTCCGAGTT TTCAGCTCAG TGGAGAAGAG CAAGCTTCCC
    TCCTCACAAC TTGGACTCGT AATTTCTTCT TATTATGAAA CTATTAATGT TCTTCAAACC
    AGCAATCAAA GAAGTCTTCA TGTGCAGGCG TTGCATGAAC TTGGAAATCT TCTCATCTTC
    GCACAGAAGA AAAGGGCTGC TTTTAAGTGT TGGAGTCAAG CTCTGGATGA AATTTTCAGA
    AAAGAAGATG TGCTCCATAC ATGGAAGGAG ATTGGCAGCT CACTCACCAG TGCCACTGAT
    GGCTGGTCAC CTCCAGGTTC CAAAGACTAC AGTGAGGAGC TCCTGTCCAA AGGCGGCATC
    TGGGGGTGTT TGCAGGGAGC GGTGATATCA GCAAAGATAG CACAATTTAT TCAGACATTG
    GATGTTGAAA AGAGAACCAA TTGTTGCCTT TTATCAGCAT TACTCTTTCA GGGTTTGCTT
    AGAACCACAC TGCCGCATCC TAAAGCTGAA CGTTGCTACG CTCAGTATGA AATCACTCAG
    CTTCTCCCGG GCATCGAGCT CTTCTCAGAC AGATATCGGG CTGACATCTG CTCCGTCATT
    GCGAGTTTAT ATTACGTCAT CCGCGAGCTG CACTTTGCTA AGCAAAATCT AGTAATTCTG
    CCTCTCCTTG CATTGTACCA ATATTTTGTT TCTGTAATTT GCCAAGACCT AGTCAAAAAT
    CTGGAAGCAA GAATCCTCAA GATAGAAGTC CTTACAGATT TAGGATTCTT TTCCGAAGCC
    TTTCATGAGC TATCCCAGAT ATTTTATGCG AAAAACACAC CTTCTTCAGT ACCCACCAGC
    TGTAAACCTA CTGGAAAAAT GAAGCTATTT CAATCATTTG ACTCGGGAAA ACCTCTTATT
    AGTAAAGAGA ATCTGCAGGC ACTTGATGAT TTAATAAATA AAGGAATGCC TGTAATTCTG
    ACTACAGTCA GCCAGCAACA TCTCTTAAAT AAGTTTTTAG TTGTTAAAGC ATACTTTTTC
    ATAAATGTGG CTGCAACAAT AAATTGTGTC CCAGAACAGA CAATGAAACC AGTCGGCCAT
    GGAGTTATGA ATGAGAAAAG CAAACCAAAT CTTCCAAACT TGAAAGAGAT GTGTTTAAAG
    GATGAGGGAG GCTCACTTTG TCATCTGACA AAATTGAAAG AGAACTTTGC TCTTAGCACA
    CTAAAGTCAA TTTTACTGAC AGAAGCTGAA GACAGACTCA ACACCCTTCT GTCGGAGATG
    GACCATCTAA GCCATAAGGA CCTCTCTCAG TGTTCTGCTG GCGAACTGGA GATTGTAGTG
    GAGGTCAGAC TGCAGCTGGC TGCCATTGCC CTGCAGAGAC ACCGGGCAGC ATACAGTGCT
    GCGATAGTAT TTTCCACACT TAAACTTCTC CAGGATTCAA AACTTTTTAA AAAGAAAGTA
    GTAGAAGATG ACTCAGAGAA TTGCACCTCT TCAGAAAGTT ATGCGACTGA AAGTAAAGAC
    GACAATTTTT TAGATCCTAT TTCCTTAAAT TCTCGAGAAT ATTTCAACAT TCATCTGTGG
    TTGAGGTGCC GCTTAGCCTT GGTGACTGCA TTTGTTGCCC AGATTCGTGG CATTGGAATC
    GTGAAAGAAA ACGATATAAC AGATTACGTG AGCCTTATCA ATGAAGTGTG TGTGGAGGCA
    AAAGCTGCAG GTGACAGGGA GCTACAGGCT GAATTCTTGA TGCAAGCTGT AATTATCGGC
    CTACAAGAAA AGCATTTAAA GGCAGACATC ATAAAAAGCC TTCAGGAGGT AATACAGTTG
    CTTGAAGGGC ATGACTTTAT TTCTCCTCGA TCTCATTTAA CCCTGGTGAG AAGCATGCTT
    TTGCTGGATG ATTTAACAAA AGCTGAGAAA TTCAAGGAAA CTCCTTTTTC GAAAATAGAA
    AAATTGTATT TGTTGACTCA GTCTCACAAC ATTCTTATAG AACAGATGAT AACTTTTGGA
    GAAACAATTG AATTGCCTTC ATCAAACACT GACTATGCAA GTCCATTACT GCCTTTGAAA
    AATATTTATC TTCCTCACGT CATGTTGTTG GCCAAAACAA AAATGAGAAT TGGACATACA
    GTGGCCAAGC AAGTATATTA TACCAGTAAA AAGAAGGATT CTTCAAAGTG GTTACCTGCC
    CTTCATTTTT TTGAAATAGC TTTGAAGCTC GCTAGGACAT CAGGAACAGA AGAACATGAG
    GTGGAAGCTG AAATCTTTTT TCAGAAAGGC AAAATAGAAT GTCAAATATT GATGGAAGAG
    AAACCTCCAA CTATGCAACT TGAGAGTCTG TTTGAAGCTA TACAGCTAAG CTTGAGAAAT
    GATCAAAATT CAGGGTTGAT AAGAGACTCC TACCTTGAAA TAGCCTTATT ATTTTTACAC
    ATGAGGAAGT TAAAGAGCAA ACTTTCAGTG TCACCATTGA TGAGTATTAA GGCTCTTCCC
    AGAAGGCATA GTTCTATTAA AGAACCAGTA GTAAATAAAC ATGAAATGTA CAGTTTATTT
    GCCTGGATTG CAATAAGAGC TGCTGCACAG GTCAGTGAGG CTGTGCTGGC AATTAATCTA
    CTTATTGGAA AGAAGAATGC TAGAACTGAT ACAGTTAGCC AAGGGGCATT ATCAAATATT
    CCAGAATTTG CTTCCATGGA TCTTTTGTCT TCATACACAG ATTATTTGCT TGATAACTAC
    CAAGTTGTCT TCCAGACTGG TTGTACATTT TCTTACGTTA GTGAAGAGAT ACATGAAGAT
    GTAGATTCTC AGAAAAAAAA CCCAATTAAA GTGGATATTA CATGGATTCT TCTGCTGCGC
    TACTATATTC ACCTACAAAG AATTAATAAT ATGAGCAAAT TGCTAGGTTT TATTGTTGTA
    TGCATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89899
    Clone ID Related Accession (Same CDS sequence) XM_015471145.1 , XM_015463358.1
    Accession Version XM_015471145.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9591bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGACAGGAG TAACCAATGC TTCTCTATGC
    TCCGATTTAT CACTAAAGAC CGTTAGCAGC ACATTGTATG GAGACGTGGT GCCACCAGTT
    GTGGCAACAC AGGCTTTAAA CAAAGAACTT TGTTTTCAGT GGTACATTCC TCCCCTGGAA
    AAACCTCCAA AGGAAACAGA ACCTATGGTT TTATTGTTGT ATGCATATAA TATGAAGCCC
    CTGAATATTT CTGATGTTGC ATCTGCTGCT TGCAACTGTG TGTACGTAGG CTCTTTCTGG
    GTTCCACTAA AAAGAGTTAT TTCAGTTCAT GAGAAACTAT CTAATCTTAC GCAAATTGCT
    GAACTATCAT TGCCAACAAC TCCTAAAGTT ACTTCAAATG AAAATTTACA TGAAGTAGAG
    GAAACAGAAG AGAAATCAAT TGATGCAGAA ATGGAAAACA TGATTATTGT CTGTTGCAAT
    GAAATAGTAT CTTTGTTTTT GGGTGATGAA ATGGCAACTC CACTATCTGA GGTCCCATTT
    GATGTCTCGC TGCCATCTAT ATTCAGTCTG GAGAGACTTT TTGATCTTGC TAGTGGCTGT
    ATCGTATCGG GAGGAAGCCT TTTCAACTGG GTGGTATCAA TTATTCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326631.1
    CDS16..9606
    Translation

    Target ORF information:

    RefSeq Version XM_015471145.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471145.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGACAGGAG TAACCAATGC TTCTCTATGC
    TCCGATTTAT CACTAAAGAC CGTTAGCAGC ACATTGTATG GAGACGTGGT GCCACCAGTT
    GTGGCAACAC AGGCTTTAAA CAAAGAACTT TGTTTTCAGT GGTACATTCC TCCCCTGGAA
    AAACCTCCAA AGGAAACAGA ACCTATGGTT TTATTGTTGT ATGCATATAA TATGAAGCCC
    CTGAATATTT CTGATGTTGC ATCTGCTGCT TGCAACTGTG TGTACGTAGG CTCTTTCTGG
    GTTCCACTAA AAAGAGTTAT TTCAGTTCAT GAGAAACTAT CTAATCTTAC GCAAATTGCT
    GAACTATCAT TGCCAACAAC TCCTAAAGTT ACTTCAAATG AAAATTTACA TGAAGTAGAG
    GAAACAGAAG AGAAATCAAT TGATGCAGAA ATGGAAAACA TGATTATTGT CTGTTGCAAT
    GAAATAGTAT CTTTGTTTTT GGGTGATGAA ATGGCAACTC CACTATCTGA GGTCCCATTT
    GATGTCTCGC TGCCATCTAT ATTCAGTCTG GAGAGACTTT TTGATCTTGC TAGTGGCTGT
    ATCGTATCGG GAGGAAGCCT TTTCAACTGG GTGGTATCAA TTATTCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89899
    Clone ID Related Accession (Same CDS sequence) XM_015471145.1 , XM_015463358.1
    Accession Version XM_015463358.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9591bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644803.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGACAGGAG TAACCAATGC TTCTCTATGC
    TCCGATTTAT CACTAAAGAC CGTTAGCAGC ACATTGTATG GAGACGTGGT GCCACCAGTT
    GTGGCAACAC AGGCTTTAAA CAAAGAACTT TGTTTTCAGT GGTACATTCC TCCCCTGGAA
    AAACCTCCAA AGGAAACAGA ACCTATGGTT TTATTGTTGT ATGCATATAA TATGAAGCCC
    CTGAATATTT CTGATGTTGC ATCTGCTGCT TGCAACTGTG TGTACGTAGG CTCTTTCTGG
    GTTCCACTAA AAAGAGTTAT TTCAGTTCAT GAGAAACTAT CTAATCTTAC GCAAATTGCT
    GAACTATCAT TGCCAACAAC TCCTAAAGTT ACTTCAAATG AAAATTTACA TGAAGTAGAG
    GAAACAGAAG AGAAATCAAT TGATGCAGAA ATGGAAAACA TGATTATTGT CTGTTGCAAT
    GAAATAGTAT CTTTGTTTTT GGGTGATGAA ATGGCAACTC CACTATCTGA GGTCCCATTT
    GATGTCTCGC TGCCATCTAT ATTCAGTCTG GAGAGACTTT TTGATCTTGC TAGTGGCTGT
    ATCGTATCGG GAGGAAGCCT TTTCAACTGG GTGGTATCAA TTATTCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318844.1
    CDS16..9606
    Translation

    Target ORF information:

    RefSeq Version XM_015463358.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463358.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGACAGGAG TAACCAATGC TTCTCTATGC
    TCCGATTTAT CACTAAAGAC CGTTAGCAGC ACATTGTATG GAGACGTGGT GCCACCAGTT
    GTGGCAACAC AGGCTTTAAA CAAAGAACTT TGTTTTCAGT GGTACATTCC TCCCCTGGAA
    AAACCTCCAA AGGAAACAGA ACCTATGGTT TTATTGTTGT ATGCATATAA TATGAAGCCC
    CTGAATATTT CTGATGTTGC ATCTGCTGCT TGCAACTGTG TGTACGTAGG CTCTTTCTGG
    GTTCCACTAA AAAGAGTTAT TTCAGTTCAT GAGAAACTAT CTAATCTTAC GCAAATTGCT
    GAACTATCAT TGCCAACAAC TCCTAAAGTT ACTTCAAATG AAAATTTACA TGAAGTAGAG
    GAAACAGAAG AGAAATCAAT TGATGCAGAA ATGGAAAACA TGATTATTGT CTGTTGCAAT
    GAAATAGTAT CTTTGTTTTT GGGTGATGAA ATGGCAACTC CACTATCTGA GGTCCCATTT
    GATGTCTCGC TGCCATCTAT ATTCAGTCTG GAGAGACTTT TTGATCTTGC TAGTGGCTGT
    ATCGTATCGG GAGGAAGCCT TTTCAACTGG GTGGTATCAA TTATTCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89900
    Clone ID Related Accession (Same CDS sequence) XM_005198332.3 , XM_002687523.5 , XM_002687523.6
    Accession Version XM_005198332.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644803.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005198332.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GGAGACAAAG GAGGAGTGAA CAATTTAAAA
    TTTGCTACTG GGAAGGTGGA GTTTTCCATG GGGACAGAAG AGATGCACAT GCCAACACCC
    CCCGACCTTT CTCAGGAGCA CTTCCGAGTT TTCAGCTCAG TGGAGAAGAG CAAGCTTCCC
    TCCTCACAAC TTGGACTCGT AATTTCTTCT TATTATGAAA CTATTAATGT TCTTCAAACC
    AGCAATCAAA GAAGTCTTCA TGTGCAGGCG TTGCATGAAC TTGGAAATCT TCTCATCTTC
    GCACAGAAGA AAAGGGCTGC TTTTAAGTGT TGGAGTCAAG CTCTGGATGA AATTTTCAGA
    AAAGAAGATG TGCTCCATAC ATGGAAGGAG ATTGGCAGCT CACTCACCAG TGCCACTGAT
    GGCTGGTCAC CTCCAGGTTC CAAAGACTAC AGTGAGGAGC TCCTGTCCAA AGGCGGCATC
    TGGGGGTGTT TGCAGGGAGC GGTGATATCA GCAAAGATAG CACAATTTAT TCAGACATTG
    GATGTTGAAA AGAGAACCAA TTGTTGCCTT TTATCAGCAT TACTCTTTCA GGGTTTGCTT
    AGAACCACAC TGCCGCATCC TAAAGCTGAA CGTTGCTACG CTCAGTATGA AATCACTCAG
    CTTCTCCCGG GCATCGAGCT CTTCTCAGAC AGATATCGGG CTGACATCTG CTCCGTCATT
    GCGAGTTTAT ATTACGTCAT CCGCGAGCTG CACTTTGCTA AGCAAAATCT AGTAATTCTG
    CCTCTCCTTG CATTGTACCA ATATTTTGTT TCTGTAATTT GCCAAGACCT AGTCAAAAAT
    CTGGAAGCAA GAATCCTCAA GATAGAAGTC CTTACAGATT TAGGATTCTT TTCCGAAGCC
    TTTCATGAGC TATCCCAGAT ATTTTATGCG AAAAACACAC CTTCTTCAGT ACCCACCAGC
    TGTAAACCTA CTGGAAAAAT GAAGCTATTT CAATCATTTG ACTCGGGAAA ACCTCTTATT
    AGTAAAGAGA ATCTGCAGGC ACTTGATGAT TTAATAAATA AAGGAATGCC TGTAATTCTG
    ACTACAGTCA GCCAGCAACA TCTCTTAAAT AAGTTTTTAG TTGTTAAAGC ATACTTTTTC
    ATAAATGTGG CTGCAACAAT AAATTGTGTC CCAGAACAGA CAATGAAACC AGTCGGCCAT
    GGAGTTATGA ATGAGAAAAG CAAACCAAAT CTTCCAAACT TGAAAGAGAT GTGTTTAAAG
    GATGAGGGAG GCTCACTTTG TCATCTGACA AAATTGAAAG AGAACTTTGC TCTTAGCACA
    CTAAAGTCAA TTTTACTGAC AGAAGCTGAA GACAGACTCA ACACCCTTCT GTCGGAGATG
    GACCATCTAA GCCATAAGGA CCTCTCTCAG TGTTCTGCTG GCGAACTGGA GATTGTAGTG
    GAGGTCAGAC TGCAGCTGGC TGCCATTGCC CTGCAGAGAC ACCGGGCAGC ATACAGTGCT
    GCGATAGTAT TTTCCACACT TAAACTTCTC CAGGATTCAA AACTTTTTAA AAAGAAAGTA
    GTAGAAGATG ACTCAGAGAA TTGCACCTCT TCAGAAAGTT ATGCGACTGA AAGTAAAGAC
    GACAATTTTT TAGATCCTAT TTCCTTAAAT TCTCGAGAAT ATTTCAACAT TCATCTGTGG
    TTGAGGTGCC GCTTAGCCTT GGTGACTGCA TTTGTTGCCC AGATTCGTGG CATTGGAATC
    GTGAAAGAAA ACGATATAAC AGATTACGTG AGCCTTATCA ATGAAGTGTG TGTGGAGGCA
    AAAGCTGCAG GTGACAGGGA GCTACAGGCT GAATTCTTGA TGCAAGCTGT AATTATCGGC
    CTACAAGAAA AGCATTTAAA GGCAGACATC ATAAAAAGCC TTCAGGAGGT AATACAGTTG
    CTTGAAGGGC ATGACTTTAT TTCTCCTCGA TCTCATTTAA CCCTGGTGAG AAGCATGCTT
    TTGCTGGATG ATTTAACAAA AGCTGAGAAA TTCAAGGAAA CTCCTTTTTC GAAAATAGAA
    AAATTGTATT TGTTGACTCA GTCTCACAAC ATTCTTATAG AACAGATGAT AACTTTTGGA
    GAAACAATTG AATTGCCTTC ATCAAACACT GACTATGCAA GTCCATTACT GCCTTTGAAA
    AATATTTATC TTCCTCACGT CATGTTGTTG GCCAAAACAA AAATGAGAAT TGGACATACA
    GTGGCCAAGC AAGTATATTA TACCAGTAAA AAGAAGGATT CTTCAAAGTG GTTACCTGCC
    CTTCATTTTT TTGAAATAGC TTTGAAGCTC GCTAGGACAT CAGGAACAGA AGAACATGAG
    GTGGAAGCTG AAATCTTTTT TCAGAAAGGC AAAATAGAAT GTCAAATATT GATGGAAGAG
    AAACCTCCAA CTATGCAACT TGAGAGTCTG TTTGAAGCTA TACAGCTAAG CTTGAGAAAT
    GATCAAAATT CAGGGTTGAT AAGAGACTCC TACCTTGAAA TAGCCTTATT ATTTTTACAC
    ATGAGGAAGT TAAAGAGCAA ACTTTCAGTG TCACCATTGA TGAGTATTAA GGCTCTTCCC
    AGAAGGCATA GTTCTATTAA AGAACCAGTA GTAAATAAAC ATGAAATGTA CAGTTTATTT
    GCCTGGATTG CAATAAGAGC TGCTGCACAG GTCAGTGAGG CTGTGCTGGC AATTAATCTA
    CTTATTGGAA AGAAGAATGC TAGAACTGAT ACAGTTAGCC AAGGGGCATT ATCAAATATT
    CCAGAATTTG CTTCCATGGA TCTTTTGTCT TCATACACAG ATTATTTGCT TGATAACTAC
    CAAGTTGTCT TCCAGACTGG TTGTACATTT TCTTACGTTA GTGAAGAGAT ACATGAAGAT
    GTAGATTCTC AGAAAAAAAA CCCAATTAAA GTGGATATTA CATGGATTCT TCTGCTGCGC
    TACTATATTC ACCTACAAAG AATTAATAAT ATGAGCAAAT TGCTAGCATC TCTGAAGCCA
    GGATCTGGGA TGTCTTTGCC AGATGACACT CTTCTCACTT CTCTTTTTAA CTCTGGATTG
    ATTTTGCGCC AAAAAGAAAT GCATTTTTTC CTTAAAAGAT TCTTACAGCT ATATTCTTCT
    TCTTGTATTG ATGAATTTCC AAAAGAACTA TTTCAAGTCA TGGAACATCC ACCTGTTCTA
    GAAAAAGTCT TATATGATTC AGCAAGCAAG ACAGGAGTAA CCAATGCTTC TCTATGCTCC
    GATTTATCAC TAAAGACCGT TAGCAGCACA TTGTATGGAG ACGTGGTGCC ACCAGTTGTG
    GCAACACAGG CTTTAAACAA AGAACTTTGT TTTCAGTGGT ACATTCCTCC CCTGGAAAAA
    CCTCCAAAGG AAACAGAACC TATGGTTTTA TTGTTGTATG CATATAATAT GAAGCCCCTG
    AATATTTCTG ATGTTGCATC TGCTGCTTGC AACTGTGTGT ACGTAGGCTC TTTCTGGGTT
    CCACTAAAAA GAGTTATTTC AGTTCATGAG AAACTATCTA ATCTTACGCA AATTGCTGAA
    CTATCATTGC CAACAACTCC TAAAGTTACT TCAAATGAAA ATTTACATGA AGTAGAGGAA
    ACAGAAGAGA AATCAATTGA TGCAGAAATG GAAAACATGA TTATTGTCTG TTGCAATGAA
    ATAGTATCTT TGTTTTTGGG TGATGAAATG GCAACTCCAC TATCTGAGGT CCCATTTGAT
    GTCTCGCTGC CATCTATATT CAGTCTGGAG AGACTTTTTG ATCTTGCTAG TGGCTGTATC
    GTATCGGGAG GAAGCCTTTT CAACTGGGTG GTATCAATTA TTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005198389.3
    CDS16..9603
    Translation

    Target ORF information:

    RefSeq Version XM_005198332.3
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005198332.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GGAGACAAAG GAGGAGTGAA CAATTTAAAA
    TTTGCTACTG GGAAGGTGGA GTTTTCCATG GGGACAGAAG AGATGCACAT GCCAACACCC
    CCCGACCTTT CTCAGGAGCA CTTCCGAGTT TTCAGCTCAG TGGAGAAGAG CAAGCTTCCC
    TCCTCACAAC TTGGACTCGT AATTTCTTCT TATTATGAAA CTATTAATGT TCTTCAAACC
    AGCAATCAAA GAAGTCTTCA TGTGCAGGCG TTGCATGAAC TTGGAAATCT TCTCATCTTC
    GCACAGAAGA AAAGGGCTGC TTTTAAGTGT TGGAGTCAAG CTCTGGATGA AATTTTCAGA
    AAAGAAGATG TGCTCCATAC ATGGAAGGAG ATTGGCAGCT CACTCACCAG TGCCACTGAT
    GGCTGGTCAC CTCCAGGTTC CAAAGACTAC AGTGAGGAGC TCCTGTCCAA AGGCGGCATC
    TGGGGGTGTT TGCAGGGAGC GGTGATATCA GCAAAGATAG CACAATTTAT TCAGACATTG
    GATGTTGAAA AGAGAACCAA TTGTTGCCTT TTATCAGCAT TACTCTTTCA GGGTTTGCTT
    AGAACCACAC TGCCGCATCC TAAAGCTGAA CGTTGCTACG CTCAGTATGA AATCACTCAG
    CTTCTCCCGG GCATCGAGCT CTTCTCAGAC AGATATCGGG CTGACATCTG CTCCGTCATT
    GCGAGTTTAT ATTACGTCAT CCGCGAGCTG CACTTTGCTA AGCAAAATCT AGTAATTCTG
    CCTCTCCTTG CATTGTACCA ATATTTTGTT TCTGTAATTT GCCAAGACCT AGTCAAAAAT
    CTGGAAGCAA GAATCCTCAA GATAGAAGTC CTTACAGATT TAGGATTCTT TTCCGAAGCC
    TTTCATGAGC TATCCCAGAT ATTTTATGCG AAAAACACAC CTTCTTCAGT ACCCACCAGC
    TGTAAACCTA CTGGAAAAAT GAAGCTATTT CAATCATTTG ACTCGGGAAA ACCTCTTATT
    AGTAAAGAGA ATCTGCAGGC ACTTGATGAT TTAATAAATA AAGGAATGCC TGTAATTCTG
    ACTACAGTCA GCCAGCAACA TCTCTTAAAT AAGTTTTTAG TTGTTAAAGC ATACTTTTTC
    ATAAATGTGG CTGCAACAAT AAATTGTGTC CCAGAACAGA CAATGAAACC AGTCGGCCAT
    GGAGTTATGA ATGAGAAAAG CAAACCAAAT CTTCCAAACT TGAAAGAGAT GTGTTTAAAG
    GATGAGGGAG GCTCACTTTG TCATCTGACA AAATTGAAAG AGAACTTTGC TCTTAGCACA
    CTAAAGTCAA TTTTACTGAC AGAAGCTGAA GACAGACTCA ACACCCTTCT GTCGGAGATG
    GACCATCTAA GCCATAAGGA CCTCTCTCAG TGTTCTGCTG GCGAACTGGA GATTGTAGTG
    GAGGTCAGAC TGCAGCTGGC TGCCATTGCC CTGCAGAGAC ACCGGGCAGC ATACAGTGCT
    GCGATAGTAT TTTCCACACT TAAACTTCTC CAGGATTCAA AACTTTTTAA AAAGAAAGTA
    GTAGAAGATG ACTCAGAGAA TTGCACCTCT TCAGAAAGTT ATGCGACTGA AAGTAAAGAC
    GACAATTTTT TAGATCCTAT TTCCTTAAAT TCTCGAGAAT ATTTCAACAT TCATCTGTGG
    TTGAGGTGCC GCTTAGCCTT GGTGACTGCA TTTGTTGCCC AGATTCGTGG CATTGGAATC
    GTGAAAGAAA ACGATATAAC AGATTACGTG AGCCTTATCA ATGAAGTGTG TGTGGAGGCA
    AAAGCTGCAG GTGACAGGGA GCTACAGGCT GAATTCTTGA TGCAAGCTGT AATTATCGGC
    CTACAAGAAA AGCATTTAAA GGCAGACATC ATAAAAAGCC TTCAGGAGGT AATACAGTTG
    CTTGAAGGGC ATGACTTTAT TTCTCCTCGA TCTCATTTAA CCCTGGTGAG AAGCATGCTT
    TTGCTGGATG ATTTAACAAA AGCTGAGAAA TTCAAGGAAA CTCCTTTTTC GAAAATAGAA
    AAATTGTATT TGTTGACTCA GTCTCACAAC ATTCTTATAG AACAGATGAT AACTTTTGGA
    GAAACAATTG AATTGCCTTC ATCAAACACT GACTATGCAA GTCCATTACT GCCTTTGAAA
    AATATTTATC TTCCTCACGT CATGTTGTTG GCCAAAACAA AAATGAGAAT TGGACATACA
    GTGGCCAAGC AAGTATATTA TACCAGTAAA AAGAAGGATT CTTCAAAGTG GTTACCTGCC
    CTTCATTTTT TTGAAATAGC TTTGAAGCTC GCTAGGACAT CAGGAACAGA AGAACATGAG
    GTGGAAGCTG AAATCTTTTT TCAGAAAGGC AAAATAGAAT GTCAAATATT GATGGAAGAG
    AAACCTCCAA CTATGCAACT TGAGAGTCTG TTTGAAGCTA TACAGCTAAG CTTGAGAAAT
    GATCAAAATT CAGGGTTGAT AAGAGACTCC TACCTTGAAA TAGCCTTATT ATTTTTACAC
    ATGAGGAAGT TAAAGAGCAA ACTTTCAGTG TCACCATTGA TGAGTATTAA GGCTCTTCCC
    AGAAGGCATA GTTCTATTAA AGAACCAGTA GTAAATAAAC ATGAAATGTA CAGTTTATTT
    GCCTGGATTG CAATAAGAGC TGCTGCACAG GTCAGTGAGG CTGTGCTGGC AATTAATCTA
    CTTATTGGAA AGAAGAATGC TAGAACTGAT ACAGTTAGCC AAGGGGCATT ATCAAATATT
    CCAGAATTTG CTTCCATGGA TCTTTTGTCT TCATACACAG ATTATTTGCT TGATAACTAC
    CAAGTTGTCT TCCAGACTGG TTGTACATTT TCTTACGTTA GTGAAGAGAT ACATGAAGAT
    GTAGATTCTC AGAAAAAAAA CCCAATTAAA GTGGATATTA CATGGATTCT TCTGCTGCGC
    TACTATATTC ACCTACAAAG AATTAATAAT ATGAGCAAAT TGCTAGCATC TCTGAAGCCA
    GGATCTGGGA TGTCTTTGCC AGATGACACT CTTCTCACTT CTCTTTTTAA CTCTGGATTG
    ATTTTGCGCC AAAAAGAAAT GCATTTTTTC CTTAAAAGAT TCTTACAGCT ATATTCTTCT
    TCTTGTATTG ATGAATTTCC AAAAGAACTA TTTCAAGTCA TGGAACATCC ACCTGTTCTA
    GAAAAAGTCT TATATGATTC AGCAAGCAAG ACAGGAGTAA CCAATGCTTC TCTATGCTCC
    GATTTATCAC TAAAGACCGT TAGCAGCACA TTGTATGGAG ACGTGGTGCC ACCAGTTGTG
    GCAACACAGG CTTTAAACAA AGAACTTTGT TTTCAGTGGT ACATTCCTCC CCTGGAAAAA
    CCTCCAAAGG AAACAGAACC TATGGTTTTA TTGTTGTATG CATATAATAT GAAGCCCCTG
    AATATTTCTG ATGTTGCATC TGCTGCTTGC AACTGTGTGT ACGTAGGCTC TTTCTGGGTT
    CCACTAAAAA GAGTTATTTC AGTTCATGAG AAACTATCTA ATCTTACGCA AATTGCTGAA
    CTATCATTGC CAACAACTCC TAAAGTTACT TCAAATGAAA ATTTACATGA AGTAGAGGAA
    ACAGAAGAGA AATCAATTGA TGCAGAAATG GAAAACATGA TTATTGTCTG TTGCAATGAA
    ATAGTATCTT TGTTTTTGGG TGATGAAATG GCAACTCCAC TATCTGAGGT CCCATTTGAT
    GTCTCGCTGC CATCTATATT CAGTCTGGAG AGACTTTTTG ATCTTGCTAG TGGCTGTATC
    GTATCGGGAG GAAGCCTTTT CAACTGGGTG GTATCAATTA TTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89900
    Clone ID Related Accession (Same CDS sequence) XM_005198332.3 , XM_002687523.5 , XM_002687523.6
    Accession Version XM_002687523.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002687523.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GGAGACAAAG GAGGAGTGAA CAATTTAAAA
    TTTGCTACTG GGAAGGTGGA GTTTTCCATG GGGACAGAAG AGATGCACAT GCCAACACCC
    CCCGACCTTT CTCAGGAGCA CTTCCGAGTT TTCAGCTCAG TGGAGAAGAG CAAGCTTCCC
    TCCTCACAAC TTGGACTCGT AATTTCTTCT TATTATGAAA CTATTAATGT TCTTCAAACC
    AGCAATCAAA GAAGTCTTCA TGTGCAGGCG TTGCATGAAC TTGGAAATCT TCTCATCTTC
    GCACAGAAGA AAAGGGCTGC TTTTAAGTGT TGGAGTCAAG CTCTGGATGA AATTTTCAGA
    AAAGAAGATG TGCTCCATAC ATGGAAGGAG ATTGGCAGCT CACTCACCAG TGCCACTGAT
    GGCTGGTCAC CTCCAGGTTC CAAAGACTAC AGTGAGGAGC TCCTGTCCAA AGGCGGCATC
    TGGGGGTGTT TGCAGGGAGC GGTGATATCA GCAAAGATAG CACAATTTAT TCAGACATTG
    GATGTTGAAA AGAGAACCAA TTGTTGCCTT TTATCAGCAT TACTCTTTCA GGGTTTGCTT
    AGAACCACAC TGCCGCATCC TAAAGCTGAA CGTTGCTACG CTCAGTATGA AATCACTCAG
    CTTCTCCCGG GCATCGAGCT CTTCTCAGAC AGATATCGGG CTGACATCTG CTCCGTCATT
    GCGAGTTTAT ATTACGTCAT CCGCGAGCTG CACTTTGCTA AGCAAAATCT AGTAATTCTG
    CCTCTCCTTG CATTGTACCA ATATTTTGTT TCTGTAATTT GCCAAGACCT AGTCAAAAAT
    CTGGAAGCAA GAATCCTCAA GATAGAAGTC CTTACAGATT TAGGATTCTT TTCCGAAGCC
    TTTCATGAGC TATCCCAGAT ATTTTATGCG AAAAACACAC CTTCTTCAGT ACCCACCAGC
    TGTAAACCTA CTGGAAAAAT GAAGCTATTT CAATCATTTG ACTCGGGAAA ACCTCTTATT
    AGTAAAGAGA ATCTGCAGGC ACTTGATGAT TTAATAAATA AAGGAATGCC TGTAATTCTG
    ACTACAGTCA GCCAGCAACA TCTCTTAAAT AAGTTTTTAG TTGTTAAAGC ATACTTTTTC
    ATAAATGTGG CTGCAACAAT AAATTGTGTC CCAGAACAGA CAATGAAACC AGTCGGCCAT
    GGAGTTATGA ATGAGAAAAG CAAACCAAAT CTTCCAAACT TGAAAGAGAT GTGTTTAAAG
    GATGAGGGAG GCTCACTTTG TCATCTGACA AAATTGAAAG AGAACTTTGC TCTTAGCACA
    CTAAAGTCAA TTTTACTGAC AGAAGCTGAA GACAGACTCA ACACCCTTCT GTCGGAGATG
    GACCATCTAA GCCATAAGGA CCTCTCTCAG TGTTCTGCTG GCGAACTGGA GATTGTAGTG
    GAGGTCAGAC TGCAGCTGGC TGCCATTGCC CTGCAGAGAC ACCGGGCAGC ATACAGTGCT
    GCGATAGTAT TTTCCACACT TAAACTTCTC CAGGATTCAA AACTTTTTAA AAAGAAAGTA
    GTAGAAGATG ACTCAGAGAA TTGCACCTCT TCAGAAAGTT ATGCGACTGA AAGTAAAGAC
    GACAATTTTT TAGATCCTAT TTCCTTAAAT TCTCGAGAAT ATTTCAACAT TCATCTGTGG
    TTGAGGTGCC GCTTAGCCTT GGTGACTGCA TTTGTTGCCC AGATTCGTGG CATTGGAATC
    GTGAAAGAAA ACGATATAAC AGATTACGTG AGCCTTATCA ATGAAGTGTG TGTGGAGGCA
    AAAGCTGCAG GTGACAGGGA GCTACAGGCT GAATTCTTGA TGCAAGCTGT AATTATCGGC
    CTACAAGAAA AGCATTTAAA GGCAGACATC ATAAAAAGCC TTCAGGAGGT AATACAGTTG
    CTTGAAGGGC ATGACTTTAT TTCTCCTCGA TCTCATTTAA CCCTGGTGAG AAGCATGCTT
    TTGCTGGATG ATTTAACAAA AGCTGAGAAA TTCAAGGAAA CTCCTTTTTC GAAAATAGAA
    AAATTGTATT TGTTGACTCA GTCTCACAAC ATTCTTATAG AACAGATGAT AACTTTTGGA
    GAAACAATTG AATTGCCTTC ATCAAACACT GACTATGCAA GTCCATTACT GCCTTTGAAA
    AATATTTATC TTCCTCACGT CATGTTGTTG GCCAAAACAA AAATGAGAAT TGGACATACA
    GTGGCCAAGC AAGTATATTA TACCAGTAAA AAGAAGGATT CTTCAAAGTG GTTACCTGCC
    CTTCATTTTT TTGAAATAGC TTTGAAGCTC GCTAGGACAT CAGGAACAGA AGAACATGAG
    GTGGAAGCTG AAATCTTTTT TCAGAAAGGC AAAATAGAAT GTCAAATATT GATGGAAGAG
    AAACCTCCAA CTATGCAACT TGAGAGTCTG TTTGAAGCTA TACAGCTAAG CTTGAGAAAT
    GATCAAAATT CAGGGTTGAT AAGAGACTCC TACCTTGAAA TAGCCTTATT ATTTTTACAC
    ATGAGGAAGT TAAAGAGCAA ACTTTCAGTG TCACCATTGA TGAGTATTAA GGCTCTTCCC
    AGAAGGCATA GTTCTATTAA AGAACCAGTA GTAAATAAAC ATGAAATGTA CAGTTTATTT
    GCCTGGATTG CAATAAGAGC TGCTGCACAG GTCAGTGAGG CTGTGCTGGC AATTAATCTA
    CTTATTGGAA AGAAGAATGC TAGAACTGAT ACAGTTAGCC AAGGGGCATT ATCAAATATT
    CCAGAATTTG CTTCCATGGA TCTTTTGTCT TCATACACAG ATTATTTGCT TGATAACTAC
    CAAGTTGTCT TCCAGACTGG TTGTACATTT TCTTACGTTA GTGAAGAGAT ACATGAAGAT
    GTAGATTCTC AGAAAAAAAA CCCAATTAAA GTGGATATTA CATGGATTCT TCTGCTGCGC
    TACTATATTC ACCTACAAAG AATTAATAAT ATGAGCAAAT TGCTAGCATC TCTGAAGCCA
    GGATCTGGGA TGTCTTTGCC AGATGACACT CTTCTCACTT CTCTTTTTAA CTCTGGATTG
    ATTTTGCGCC AAAAAGAAAT GCATTTTTTC CTTAAAAGAT TCTTACAGCT ATATTCTTCT
    TCTTGTATTG ATGAATTTCC AAAAGAACTA TTTCAAGTCA TGGAACATCC ACCTGTTCTA
    GAAAAAGTCT TATATGATTC AGCAAGCAAG ACAGGAGTAA CCAATGCTTC TCTATGCTCC
    GATTTATCAC TAAAGACCGT TAGCAGCACA TTGTATGGAG ACGTGGTGCC ACCAGTTGTG
    GCAACACAGG CTTTAAACAA AGAACTTTGT TTTCAGTGGT ACATTCCTCC CCTGGAAAAA
    CCTCCAAAGG AAACAGAACC TATGGTTTTA TTGTTGTATG CATATAATAT GAAGCCCCTG
    AATATTTCTG ATGTTGCATC TGCTGCTTGC AACTGTGTGT ACGTAGGCTC TTTCTGGGTT
    CCACTAAAAA GAGTTATTTC AGTTCATGAG AAACTATCTA ATCTTACGCA AATTGCTGAA
    CTATCATTGC CAACAACTCC TAAAGTTACT TCAAATGAAA ATTTACATGA AGTAGAGGAA
    ACAGAAGAGA AATCAATTGA TGCAGAAATG GAAAACATGA TTATTGTCTG TTGCAATGAA
    ATAGTATCTT TGTTTTTGGG TGATGAAATG GCAACTCCAC TATCTGAGGT CCCATTTGAT
    GTCTCGCTGC CATCTATATT CAGTCTGGAG AGACTTTTTG ATCTTGCTAG TGGCTGTATC
    GTATCGGGAG GAAGCCTTTT CAACTGGGTG GTATCAATTA TTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002687569.5
    CDS16..9603
    Translation

    Target ORF information:

    RefSeq Version XM_002687523.5
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002687523.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GGAGACAAAG GAGGAGTGAA CAATTTAAAA
    TTTGCTACTG GGAAGGTGGA GTTTTCCATG GGGACAGAAG AGATGCACAT GCCAACACCC
    CCCGACCTTT CTCAGGAGCA CTTCCGAGTT TTCAGCTCAG TGGAGAAGAG CAAGCTTCCC
    TCCTCACAAC TTGGACTCGT AATTTCTTCT TATTATGAAA CTATTAATGT TCTTCAAACC
    AGCAATCAAA GAAGTCTTCA TGTGCAGGCG TTGCATGAAC TTGGAAATCT TCTCATCTTC
    GCACAGAAGA AAAGGGCTGC TTTTAAGTGT TGGAGTCAAG CTCTGGATGA AATTTTCAGA
    AAAGAAGATG TGCTCCATAC ATGGAAGGAG ATTGGCAGCT CACTCACCAG TGCCACTGAT
    GGCTGGTCAC CTCCAGGTTC CAAAGACTAC AGTGAGGAGC TCCTGTCCAA AGGCGGCATC
    TGGGGGTGTT TGCAGGGAGC GGTGATATCA GCAAAGATAG CACAATTTAT TCAGACATTG
    GATGTTGAAA AGAGAACCAA TTGTTGCCTT TTATCAGCAT TACTCTTTCA GGGTTTGCTT
    AGAACCACAC TGCCGCATCC TAAAGCTGAA CGTTGCTACG CTCAGTATGA AATCACTCAG
    CTTCTCCCGG GCATCGAGCT CTTCTCAGAC AGATATCGGG CTGACATCTG CTCCGTCATT
    GCGAGTTTAT ATTACGTCAT CCGCGAGCTG CACTTTGCTA AGCAAAATCT AGTAATTCTG
    CCTCTCCTTG CATTGTACCA ATATTTTGTT TCTGTAATTT GCCAAGACCT AGTCAAAAAT
    CTGGAAGCAA GAATCCTCAA GATAGAAGTC CTTACAGATT TAGGATTCTT TTCCGAAGCC
    TTTCATGAGC TATCCCAGAT ATTTTATGCG AAAAACACAC CTTCTTCAGT ACCCACCAGC
    TGTAAACCTA CTGGAAAAAT GAAGCTATTT CAATCATTTG ACTCGGGAAA ACCTCTTATT
    AGTAAAGAGA ATCTGCAGGC ACTTGATGAT TTAATAAATA AAGGAATGCC TGTAATTCTG
    ACTACAGTCA GCCAGCAACA TCTCTTAAAT AAGTTTTTAG TTGTTAAAGC ATACTTTTTC
    ATAAATGTGG CTGCAACAAT AAATTGTGTC CCAGAACAGA CAATGAAACC AGTCGGCCAT
    GGAGTTATGA ATGAGAAAAG CAAACCAAAT CTTCCAAACT TGAAAGAGAT GTGTTTAAAG
    GATGAGGGAG GCTCACTTTG TCATCTGACA AAATTGAAAG AGAACTTTGC TCTTAGCACA
    CTAAAGTCAA TTTTACTGAC AGAAGCTGAA GACAGACTCA ACACCCTTCT GTCGGAGATG
    GACCATCTAA GCCATAAGGA CCTCTCTCAG TGTTCTGCTG GCGAACTGGA GATTGTAGTG
    GAGGTCAGAC TGCAGCTGGC TGCCATTGCC CTGCAGAGAC ACCGGGCAGC ATACAGTGCT
    GCGATAGTAT TTTCCACACT TAAACTTCTC CAGGATTCAA AACTTTTTAA AAAGAAAGTA
    GTAGAAGATG ACTCAGAGAA TTGCACCTCT TCAGAAAGTT ATGCGACTGA AAGTAAAGAC
    GACAATTTTT TAGATCCTAT TTCCTTAAAT TCTCGAGAAT ATTTCAACAT TCATCTGTGG
    TTGAGGTGCC GCTTAGCCTT GGTGACTGCA TTTGTTGCCC AGATTCGTGG CATTGGAATC
    GTGAAAGAAA ACGATATAAC AGATTACGTG AGCCTTATCA ATGAAGTGTG TGTGGAGGCA
    AAAGCTGCAG GTGACAGGGA GCTACAGGCT GAATTCTTGA TGCAAGCTGT AATTATCGGC
    CTACAAGAAA AGCATTTAAA GGCAGACATC ATAAAAAGCC TTCAGGAGGT AATACAGTTG
    CTTGAAGGGC ATGACTTTAT TTCTCCTCGA TCTCATTTAA CCCTGGTGAG AAGCATGCTT
    TTGCTGGATG ATTTAACAAA AGCTGAGAAA TTCAAGGAAA CTCCTTTTTC GAAAATAGAA
    AAATTGTATT TGTTGACTCA GTCTCACAAC ATTCTTATAG AACAGATGAT AACTTTTGGA
    GAAACAATTG AATTGCCTTC ATCAAACACT GACTATGCAA GTCCATTACT GCCTTTGAAA
    AATATTTATC TTCCTCACGT CATGTTGTTG GCCAAAACAA AAATGAGAAT TGGACATACA
    GTGGCCAAGC AAGTATATTA TACCAGTAAA AAGAAGGATT CTTCAAAGTG GTTACCTGCC
    CTTCATTTTT TTGAAATAGC TTTGAAGCTC GCTAGGACAT CAGGAACAGA AGAACATGAG
    GTGGAAGCTG AAATCTTTTT TCAGAAAGGC AAAATAGAAT GTCAAATATT GATGGAAGAG
    AAACCTCCAA CTATGCAACT TGAGAGTCTG TTTGAAGCTA TACAGCTAAG CTTGAGAAAT
    GATCAAAATT CAGGGTTGAT AAGAGACTCC TACCTTGAAA TAGCCTTATT ATTTTTACAC
    ATGAGGAAGT TAAAGAGCAA ACTTTCAGTG TCACCATTGA TGAGTATTAA GGCTCTTCCC
    AGAAGGCATA GTTCTATTAA AGAACCAGTA GTAAATAAAC ATGAAATGTA CAGTTTATTT
    GCCTGGATTG CAATAAGAGC TGCTGCACAG GTCAGTGAGG CTGTGCTGGC AATTAATCTA
    CTTATTGGAA AGAAGAATGC TAGAACTGAT ACAGTTAGCC AAGGGGCATT ATCAAATATT
    CCAGAATTTG CTTCCATGGA TCTTTTGTCT TCATACACAG ATTATTTGCT TGATAACTAC
    CAAGTTGTCT TCCAGACTGG TTGTACATTT TCTTACGTTA GTGAAGAGAT ACATGAAGAT
    GTAGATTCTC AGAAAAAAAA CCCAATTAAA GTGGATATTA CATGGATTCT TCTGCTGCGC
    TACTATATTC ACCTACAAAG AATTAATAAT ATGAGCAAAT TGCTAGCATC TCTGAAGCCA
    GGATCTGGGA TGTCTTTGCC AGATGACACT CTTCTCACTT CTCTTTTTAA CTCTGGATTG
    ATTTTGCGCC AAAAAGAAAT GCATTTTTTC CTTAAAAGAT TCTTACAGCT ATATTCTTCT
    TCTTGTATTG ATGAATTTCC AAAAGAACTA TTTCAAGTCA TGGAACATCC ACCTGTTCTA
    GAAAAAGTCT TATATGATTC AGCAAGCAAG ACAGGAGTAA CCAATGCTTC TCTATGCTCC
    GATTTATCAC TAAAGACCGT TAGCAGCACA TTGTATGGAG ACGTGGTGCC ACCAGTTGTG
    GCAACACAGG CTTTAAACAA AGAACTTTGT TTTCAGTGGT ACATTCCTCC CCTGGAAAAA
    CCTCCAAAGG AAACAGAACC TATGGTTTTA TTGTTGTATG CATATAATAT GAAGCCCCTG
    AATATTTCTG ATGTTGCATC TGCTGCTTGC AACTGTGTGT ACGTAGGCTC TTTCTGGGTT
    CCACTAAAAA GAGTTATTTC AGTTCATGAG AAACTATCTA ATCTTACGCA AATTGCTGAA
    CTATCATTGC CAACAACTCC TAAAGTTACT TCAAATGAAA ATTTACATGA AGTAGAGGAA
    ACAGAAGAGA AATCAATTGA TGCAGAAATG GAAAACATGA TTATTGTCTG TTGCAATGAA
    ATAGTATCTT TGTTTTTGGG TGATGAAATG GCAACTCCAC TATCTGAGGT CCCATTTGAT
    GTCTCGCTGC CATCTATATT CAGTCTGGAG AGACTTTTTG ATCTTGCTAG TGGCTGTATC
    GTATCGGGAG GAAGCCTTTT CAACTGGGTG GTATCAATTA TTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89900
    Clone ID Related Accession (Same CDS sequence) XM_005198332.3 , XM_002687523.5 , XM_002687523.6
    Accession Version XM_002687523.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002687523.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GGAGACAAAG GAGGAGTGAA CAATTTAAAA
    TTTGCTACTG GGAAGGTGGA GTTTTCCATG GGGACAGAAG AGATGCACAT GCCAACACCC
    CCCGACCTTT CTCAGGAGCA CTTCCGAGTT TTCAGCTCAG TGGAGAAGAG CAAGCTTCCC
    TCCTCACAAC TTGGACTCGT AATTTCTTCT TATTATGAAA CTATTAATGT TCTTCAAACC
    AGCAATCAAA GAAGTCTTCA TGTGCAGGCG TTGCATGAAC TTGGAAATCT TCTCATCTTC
    GCACAGAAGA AAAGGGCTGC TTTTAAGTGT TGGAGTCAAG CTCTGGATGA AATTTTCAGA
    AAAGAAGATG TGCTCCATAC ATGGAAGGAG ATTGGCAGCT CACTCACCAG TGCCACTGAT
    GGCTGGTCAC CTCCAGGTTC CAAAGACTAC AGTGAGGAGC TCCTGTCCAA AGGCGGCATC
    TGGGGGTGTT TGCAGGGAGC GGTGATATCA GCAAAGATAG CACAATTTAT TCAGACATTG
    GATGTTGAAA AGAGAACCAA TTGTTGCCTT TTATCAGCAT TACTCTTTCA GGGTTTGCTT
    AGAACCACAC TGCCGCATCC TAAAGCTGAA CGTTGCTACG CTCAGTATGA AATCACTCAG
    CTTCTCCCGG GCATCGAGCT CTTCTCAGAC AGATATCGGG CTGACATCTG CTCCGTCATT
    GCGAGTTTAT ATTACGTCAT CCGCGAGCTG CACTTTGCTA AGCAAAATCT AGTAATTCTG
    CCTCTCCTTG CATTGTACCA ATATTTTGTT TCTGTAATTT GCCAAGACCT AGTCAAAAAT
    CTGGAAGCAA GAATCCTCAA GATAGAAGTC CTTACAGATT TAGGATTCTT TTCCGAAGCC
    TTTCATGAGC TATCCCAGAT ATTTTATGCG AAAAACACAC CTTCTTCAGT ACCCACCAGC
    TGTAAACCTA CTGGAAAAAT GAAGCTATTT CAATCATTTG ACTCGGGAAA ACCTCTTATT
    AGTAAAGAGA ATCTGCAGGC ACTTGATGAT TTAATAAATA AAGGAATGCC TGTAATTCTG
    ACTACAGTCA GCCAGCAACA TCTCTTAAAT AAGTTTTTAG TTGTTAAAGC ATACTTTTTC
    ATAAATGTGG CTGCAACAAT AAATTGTGTC CCAGAACAGA CAATGAAACC AGTCGGCCAT
    GGAGTTATGA ATGAGAAAAG CAAACCAAAT CTTCCAAACT TGAAAGAGAT GTGTTTAAAG
    GATGAGGGAG GCTCACTTTG TCATCTGACA AAATTGAAAG AGAACTTTGC TCTTAGCACA
    CTAAAGTCAA TTTTACTGAC AGAAGCTGAA GACAGACTCA ACACCCTTCT GTCGGAGATG
    GACCATCTAA GCCATAAGGA CCTCTCTCAG TGTTCTGCTG GCGAACTGGA GATTGTAGTG
    GAGGTCAGAC TGCAGCTGGC TGCCATTGCC CTGCAGAGAC ACCGGGCAGC ATACAGTGCT
    GCGATAGTAT TTTCCACACT TAAACTTCTC CAGGATTCAA AACTTTTTAA AAAGAAAGTA
    GTAGAAGATG ACTCAGAGAA TTGCACCTCT TCAGAAAGTT ATGCGACTGA AAGTAAAGAC
    GACAATTTTT TAGATCCTAT TTCCTTAAAT TCTCGAGAAT ATTTCAACAT TCATCTGTGG
    TTGAGGTGCC GCTTAGCCTT GGTGACTGCA TTTGTTGCCC AGATTCGTGG CATTGGAATC
    GTGAAAGAAA ACGATATAAC AGATTACGTG AGCCTTATCA ATGAAGTGTG TGTGGAGGCA
    AAAGCTGCAG GTGACAGGGA GCTACAGGCT GAATTCTTGA TGCAAGCTGT AATTATCGGC
    CTACAAGAAA AGCATTTAAA GGCAGACATC ATAAAAAGCC TTCAGGAGGT AATACAGTTG
    CTTGAAGGGC ATGACTTTAT TTCTCCTCGA TCTCATTTAA CCCTGGTGAG AAGCATGCTT
    TTGCTGGATG ATTTAACAAA AGCTGAGAAA TTCAAGGAAA CTCCTTTTTC GAAAATAGAA
    AAATTGTATT TGTTGACTCA GTCTCACAAC ATTCTTATAG AACAGATGAT AACTTTTGGA
    GAAACAATTG AATTGCCTTC ATCAAACACT GACTATGCAA GTCCATTACT GCCTTTGAAA
    AATATTTATC TTCCTCACGT CATGTTGTTG GCCAAAACAA AAATGAGAAT TGGACATACA
    GTGGCCAAGC AAGTATATTA TACCAGTAAA AAGAAGGATT CTTCAAAGTG GTTACCTGCC
    CTTCATTTTT TTGAAATAGC TTTGAAGCTC GCTAGGACAT CAGGAACAGA AGAACATGAG
    GTGGAAGCTG AAATCTTTTT TCAGAAAGGC AAAATAGAAT GTCAAATATT GATGGAAGAG
    AAACCTCCAA CTATGCAACT TGAGAGTCTG TTTGAAGCTA TACAGCTAAG CTTGAGAAAT
    GATCAAAATT CAGGGTTGAT AAGAGACTCC TACCTTGAAA TAGCCTTATT ATTTTTACAC
    ATGAGGAAGT TAAAGAGCAA ACTTTCAGTG TCACCATTGA TGAGTATTAA GGCTCTTCCC
    AGAAGGCATA GTTCTATTAA AGAACCAGTA GTAAATAAAC ATGAAATGTA CAGTTTATTT
    GCCTGGATTG CAATAAGAGC TGCTGCACAG GTCAGTGAGG CTGTGCTGGC AATTAATCTA
    CTTATTGGAA AGAAGAATGC TAGAACTGAT ACAGTTAGCC AAGGGGCATT ATCAAATATT
    CCAGAATTTG CTTCCATGGA TCTTTTGTCT TCATACACAG ATTATTTGCT TGATAACTAC
    CAAGTTGTCT TCCAGACTGG TTGTACATTT TCTTACGTTA GTGAAGAGAT ACATGAAGAT
    GTAGATTCTC AGAAAAAAAA CCCAATTAAA GTGGATATTA CATGGATTCT TCTGCTGCGC
    TACTATATTC ACCTACAAAG AATTAATAAT ATGAGCAAAT TGCTAGCATC TCTGAAGCCA
    GGATCTGGGA TGTCTTTGCC AGATGACACT CTTCTCACTT CTCTTTTTAA CTCTGGATTG
    ATTTTGCGCC AAAAAGAAAT GCATTTTTTC CTTAAAAGAT TCTTACAGCT ATATTCTTCT
    TCTTGTATTG ATGAATTTCC AAAAGAACTA TTTCAAGTCA TGGAACATCC ACCTGTTCTA
    GAAAAAGTCT TATATGATTC AGCAAGCAAG ACAGGAGTAA CCAATGCTTC TCTATGCTCC
    GATTTATCAC TAAAGACCGT TAGCAGCACA TTGTATGGAG ACGTGGTGCC ACCAGTTGTG
    GCAACACAGG CTTTAAACAA AGAACTTTGT TTTCAGTGGT ACATTCCTCC CCTGGAAAAA
    CCTCCAAAGG AAACAGAACC TATGGTTTTA TTGTTGTATG CATATAATAT GAAGCCCCTG
    AATATTTCTG ATGTTGCATC TGCTGCTTGC AACTGTGTGT ACGTAGGCTC TTTCTGGGTT
    CCACTAAAAA GAGTTATTTC AGTTCATGAG AAACTATCTA ATCTTACGCA AATTGCTGAA
    CTATCATTGC CAACAACTCC TAAAGTTACT TCAAATGAAA ATTTACATGA AGTAGAGGAA
    ACAGAAGAGA AATCAATTGA TGCAGAAATG GAAAACATGA TTATTGTCTG TTGCAATGAA
    ATAGTATCTT TGTTTTTGGG TGATGAAATG GCAACTCCAC TATCTGAGGT CCCATTTGAT
    GTCTCGCTGC CATCTATATT CAGTCTGGAG AGACTTTTTG ATCTTGCTAG TGGCTGTATC
    GTATCGGGAG GAAGCCTTTT CAACTGGGTG GTATCAATTA TTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002687569.5
    CDS929..10516
    Translation

    Target ORF information:

    RefSeq Version XM_002687523.6
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002687523.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GGAGACAAAG GAGGAGTGAA CAATTTAAAA
    TTTGCTACTG GGAAGGTGGA GTTTTCCATG GGGACAGAAG AGATGCACAT GCCAACACCC
    CCCGACCTTT CTCAGGAGCA CTTCCGAGTT TTCAGCTCAG TGGAGAAGAG CAAGCTTCCC
    TCCTCACAAC TTGGACTCGT AATTTCTTCT TATTATGAAA CTATTAATGT TCTTCAAACC
    AGCAATCAAA GAAGTCTTCA TGTGCAGGCG TTGCATGAAC TTGGAAATCT TCTCATCTTC
    GCACAGAAGA AAAGGGCTGC TTTTAAGTGT TGGAGTCAAG CTCTGGATGA AATTTTCAGA
    AAAGAAGATG TGCTCCATAC ATGGAAGGAG ATTGGCAGCT CACTCACCAG TGCCACTGAT
    GGCTGGTCAC CTCCAGGTTC CAAAGACTAC AGTGAGGAGC TCCTGTCCAA AGGCGGCATC
    TGGGGGTGTT TGCAGGGAGC GGTGATATCA GCAAAGATAG CACAATTTAT TCAGACATTG
    GATGTTGAAA AGAGAACCAA TTGTTGCCTT TTATCAGCAT TACTCTTTCA GGGTTTGCTT
    AGAACCACAC TGCCGCATCC TAAAGCTGAA CGTTGCTACG CTCAGTATGA AATCACTCAG
    CTTCTCCCGG GCATCGAGCT CTTCTCAGAC AGATATCGGG CTGACATCTG CTCCGTCATT
    GCGAGTTTAT ATTACGTCAT CCGCGAGCTG CACTTTGCTA AGCAAAATCT AGTAATTCTG
    CCTCTCCTTG CATTGTACCA ATATTTTGTT TCTGTAATTT GCCAAGACCT AGTCAAAAAT
    CTGGAAGCAA GAATCCTCAA GATAGAAGTC CTTACAGATT TAGGATTCTT TTCCGAAGCC
    TTTCATGAGC TATCCCAGAT ATTTTATGCG AAAAACACAC CTTCTTCAGT ACCCACCAGC
    TGTAAACCTA CTGGAAAAAT GAAGCTATTT CAATCATTTG ACTCGGGAAA ACCTCTTATT
    AGTAAAGAGA ATCTGCAGGC ACTTGATGAT TTAATAAATA AAGGAATGCC TGTAATTCTG
    ACTACAGTCA GCCAGCAACA TCTCTTAAAT AAGTTTTTAG TTGTTAAAGC ATACTTTTTC
    ATAAATGTGG CTGCAACAAT AAATTGTGTC CCAGAACAGA CAATGAAACC AGTCGGCCAT
    GGAGTTATGA ATGAGAAAAG CAAACCAAAT CTTCCAAACT TGAAAGAGAT GTGTTTAAAG
    GATGAGGGAG GCTCACTTTG TCATCTGACA AAATTGAAAG AGAACTTTGC TCTTAGCACA
    CTAAAGTCAA TTTTACTGAC AGAAGCTGAA GACAGACTCA ACACCCTTCT GTCGGAGATG
    GACCATCTAA GCCATAAGGA CCTCTCTCAG TGTTCTGCTG GCGAACTGGA GATTGTAGTG
    GAGGTCAGAC TGCAGCTGGC TGCCATTGCC CTGCAGAGAC ACCGGGCAGC ATACAGTGCT
    GCGATAGTAT TTTCCACACT TAAACTTCTC CAGGATTCAA AACTTTTTAA AAAGAAAGTA
    GTAGAAGATG ACTCAGAGAA TTGCACCTCT TCAGAAAGTT ATGCGACTGA AAGTAAAGAC
    GACAATTTTT TAGATCCTAT TTCCTTAAAT TCTCGAGAAT ATTTCAACAT TCATCTGTGG
    TTGAGGTGCC GCTTAGCCTT GGTGACTGCA TTTGTTGCCC AGATTCGTGG CATTGGAATC
    GTGAAAGAAA ACGATATAAC AGATTACGTG AGCCTTATCA ATGAAGTGTG TGTGGAGGCA
    AAAGCTGCAG GTGACAGGGA GCTACAGGCT GAATTCTTGA TGCAAGCTGT AATTATCGGC
    CTACAAGAAA AGCATTTAAA GGCAGACATC ATAAAAAGCC TTCAGGAGGT AATACAGTTG
    CTTGAAGGGC ATGACTTTAT TTCTCCTCGA TCTCATTTAA CCCTGGTGAG AAGCATGCTT
    TTGCTGGATG ATTTAACAAA AGCTGAGAAA TTCAAGGAAA CTCCTTTTTC GAAAATAGAA
    AAATTGTATT TGTTGACTCA GTCTCACAAC ATTCTTATAG AACAGATGAT AACTTTTGGA
    GAAACAATTG AATTGCCTTC ATCAAACACT GACTATGCAA GTCCATTACT GCCTTTGAAA
    AATATTTATC TTCCTCACGT CATGTTGTTG GCCAAAACAA AAATGAGAAT TGGACATACA
    GTGGCCAAGC AAGTATATTA TACCAGTAAA AAGAAGGATT CTTCAAAGTG GTTACCTGCC
    CTTCATTTTT TTGAAATAGC TTTGAAGCTC GCTAGGACAT CAGGAACAGA AGAACATGAG
    GTGGAAGCTG AAATCTTTTT TCAGAAAGGC AAAATAGAAT GTCAAATATT GATGGAAGAG
    AAACCTCCAA CTATGCAACT TGAGAGTCTG TTTGAAGCTA TACAGCTAAG CTTGAGAAAT
    GATCAAAATT CAGGGTTGAT AAGAGACTCC TACCTTGAAA TAGCCTTATT ATTTTTACAC
    ATGAGGAAGT TAAAGAGCAA ACTTTCAGTG TCACCATTGA TGAGTATTAA GGCTCTTCCC
    AGAAGGCATA GTTCTATTAA AGAACCAGTA GTAAATAAAC ATGAAATGTA CAGTTTATTT
    GCCTGGATTG CAATAAGAGC TGCTGCACAG GTCAGTGAGG CTGTGCTGGC AATTAATCTA
    CTTATTGGAA AGAAGAATGC TAGAACTGAT ACAGTTAGCC AAGGGGCATT ATCAAATATT
    CCAGAATTTG CTTCCATGGA TCTTTTGTCT TCATACACAG ATTATTTGCT TGATAACTAC
    CAAGTTGTCT TCCAGACTGG TTGTACATTT TCTTACGTTA GTGAAGAGAT ACATGAAGAT
    GTAGATTCTC AGAAAAAAAA CCCAATTAAA GTGGATATTA CATGGATTCT TCTGCTGCGC
    TACTATATTC ACCTACAAAG AATTAATAAT ATGAGCAAAT TGCTAGCATC TCTGAAGCCA
    GGATCTGGGA TGTCTTTGCC AGATGACACT CTTCTCACTT CTCTTTTTAA CTCTGGATTG
    ATTTTGCGCC AAAAAGAAAT GCATTTTTTC CTTAAAAGAT TCTTACAGCT ATATTCTTCT
    TCTTGTATTG ATGAATTTCC AAAAGAACTA TTTCAAGTCA TGGAACATCC ACCTGTTCTA
    GAAAAAGTCT TATATGATTC AGCAAGCAAG ACAGGAGTAA CCAATGCTTC TCTATGCTCC
    GATTTATCAC TAAAGACCGT TAGCAGCACA TTGTATGGAG ACGTGGTGCC ACCAGTTGTG
    GCAACACAGG CTTTAAACAA AGAACTTTGT TTTCAGTGGT ACATTCCTCC CCTGGAAAAA
    CCTCCAAAGG AAACAGAACC TATGGTTTTA TTGTTGTATG CATATAATAT GAAGCCCCTG
    AATATTTCTG ATGTTGCATC TGCTGCTTGC AACTGTGTGT ACGTAGGCTC TTTCTGGGTT
    CCACTAAAAA GAGTTATTTC AGTTCATGAG AAACTATCTA ATCTTACGCA AATTGCTGAA
    CTATCATTGC CAACAACTCC TAAAGTTACT TCAAATGAAA ATTTACATGA AGTAGAGGAA
    ACAGAAGAGA AATCAATTGA TGCAGAAATG GAAAACATGA TTATTGTCTG TTGCAATGAA
    ATAGTATCTT TGTTTTTGGG TGATGAAATG GCAACTCCAC TATCTGAGGT CCCATTTGAT
    GTCTCGCTGC CATCTATATT CAGTCTGGAG AGACTTTTTG ATCTTGCTAG TGGCTGTATC
    GTATCGGGAG GAAGCCTTTT CAACTGGGTG GTATCAATTA TTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89901
    Clone ID Related Accession (Same CDS sequence) XM_015471146.1 , XM_015463359.1
    Accession Version XM_015471146.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGGCA CTTGATGATT TAATAAATAA AGGAATGCCT
    GTAATTCTGA CTACAGTCAG CCAGCAACAT CTCTTAAATA AGTTTTTAGT TGTTAAAGCA
    TACTTTTTCA TAAATGTGGC TGCAACAATA AATTGTGTCC CAGAACAGAC AATGAAACCA
    GTCGGCCATG GAGTTATGAA TGAGAAAAGC AAACCAAATC TTCCAAACTT GAAAGAGATG
    TGTTTAAAGG ATGAGGGAGG CTCACTTTGT CATCTGACAA AATTGAAAGA GAACTTTGCT
    CTTAGCACAC TAAAGTCAAT TTTACTGACA GAAGCTGAAG ACAGACTCAA CACCCTTCTG
    TCGGAGATGG ACCATCTAAG CCATAAGGAC CTCTCTCAGT GTTCTGCTGG CGAACTGGAG
    ATTGTAGTGG AGGTCAGACT GCAGCTGGCT GCCATTGCCC TGCAGAGACA CCGGGCAGCA
    TACAGTGCTG CGATAGTATT TTCCACACTT AAACTTCTCC AGGATTCAAA ACTTTTTAAA
    AAGAAAGTAG TAGAAGATGA CTCAGAGAAT TGCACCTCTT CAGAAAGTTA TGCGACTGAA
    AGTAAAGACG ACAATTTTTT AGATCCTATT TCCTTAAATT CTCGAGAATA TTTCAACATT
    CATCTGTGGT TGAGGTGCCG CTTAGCCTTG GTGACTGCAT TTGTTGCCCA GATTCGTGGC
    ATTGGAATCG TGAAAGAAAA CGATATAACA GATTACGTGA GCCTTATCAA TGAAGTGTGT
    GTGGAGGCAA AAGCTGCAGG TGACAGGGAG CTACAGGCTG AATTCTTGAT GCAAGCTGTA
    ATTATCGGCC TACAAGAAAA GCATTTAAAG GCAGACATCA TAAAAAGCCT TCAGGAGGTA
    ATACAGTTGC TTGAAGGGCA TGACTTTATT TCTCCTCGAT CTCATTTAAC CCTGGTGAGA
    AGCATGCTTT TGCTGGATGA TTTAACAAAA GCTGAGAAAT TCAAGGAAAC TCCTTTTTCG
    AAAATAGAAA AATTGTATTT GTTGACTCAG TCTCACAACA TTCTTATAGA ACAGATGATA
    ACTTTTGGAG AAACAATTGA ATTGCCTTCA TCAAACACTG ACTATGCAAG TCCATTACTG
    CCTTTGAAAA ATATTTATCT TCCTCACGTC ATGTTGTTGG CCAAAACAAA AATGAGAATT
    GGACATACAG TGGCCAAGCA AGTATATTAT ACCAGTAAAA AGAAGGATTC TTCAAAGTGG
    TTACCTGCCC TTCATTTTTT TGAAATAGCT TTGAAGCTCG CTAGGACATC AGGAACAGAA
    GAACATGAGG TGGAAGCTGA AATCTTTTTT CAGAAAGGCA AAATAGAATG TCAAATATTG
    ATGGAAGAGA AACCTCCAAC TATGCAACTT GAGAGTCTGT TTGAAGCTAT ACAGCTAAGC
    TTGAGAAATG ATCAAAATTC AGGGTTGATA AGAGACTCCT ACCTTGAAAT AGCCTTATTA
    TTTTTACACA TGAGGAAGTT AAAGAGCAAA CTTTCAGTGT CACCATTGAT GAGTATTAAG
    GCTCTTCCCA GAAGGCATAG TTCTATTAAA GAACCAGTAG TAAATAAACA TGAAATGTAC
    AGTTTATTTG CCTGGATTGC AATAAGAGCT GCTGCACAGG TCAGTGAGGC TGTGCTGGCA
    ATTAATCTAC TTATTGGAAA GAAGAATGCT AGAACTGATA CAGTTAGCCA AGGGGCATTA
    TCAAATATTC CAGAATTTGC TTCCATGGAT CTTTTGTCTT CATACACAGA TTATTTGCTT
    GATAACTACC AAGTTGTCTT CCAGACTGGT TGTACATTTT CTTACGTTAG TGAAGAGATA
    CATGAAGATG TAGATTCTCA GAAAAAAAAC CCAATTAAAG TGGATATTAC ATGGATTCTT
    CTGCTGCGCT ACTATATTCA CCTACAAAGA ATTAATAATA TGAGCAAATT GCTAGCATCT
    CTGAAGCCAG GATCTGGGAT GTCTTTGCCA GATGACACTC TTCTCACTTC TCTTTTTAAC
    TCTGGATTGA TTTTGCGCCA AAAAGAAATG CATTTTTTCC TTAAAAGATT CTTACAGCTA
    TATTCTTCTT CTTGTATTGA TGAATTTCCA AAAGAACTAT TTCAAGTCAT GGAACATCCA
    CCTGTTCTAG AAAAAGTCTT ATATGATTCA GCAAGCAAGA CAGGAGTAAC CAATGCTTCT
    CTATGCTCCG ATTTATCACT AAAGACCGTT AGCAGCACAT TGTATGGAGA CGTGGTGCCA
    CCAGTTGTGG CAACACAGGC TTTAAACAAA GAACTTTGTT TTCAGTGGTA CATTCCTCCC
    CTGGAAAAAC CTCCAAAGGA AACAGAACCT ATGGTTTTAT TGTTGTATGC ATATAATATG
    AAGCCCCTGA ATATTTCTGA TGTTGCATCT GCTGCTTGCA ACTGTGTGTA CGTAGGCTCT
    TTCTGGGTTC CACTAAAAAG AGTTATTTCA GTTCATGAGA AACTATCTAA TCTTACGCAA
    ATTGCTGAAC TATCATTGCC AACAACTCCT AAAGTTACTT CAAATGAAAA TTTACATGAA
    GTAGAGGAAA CAGAAGAGAA ATCAATTGAT GCAGAAATGG AAAACATGAT TATTGTCTGT
    TGCAATGAAA TAGTATCTTT GTTTTTGGGT GATGAAATGG CAACTCCACT ATCTGAGGTC
    CCATTTGATG TCTCGCTGCC ATCTATATTC AGTCTGGAGA GACTTTTTGA TCTTGCTAGT
    GGCTGTATCG TATCGGGAGG AAGCCTTTTC AACTGGGTGG TATCAATTAT TCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326632.1
    CDS17..9553
    Translation

    Target ORF information:

    RefSeq Version XM_015471146.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471146.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGGCA CTTGATGATT TAATAAATAA AGGAATGCCT
    GTAATTCTGA CTACAGTCAG CCAGCAACAT CTCTTAAATA AGTTTTTAGT TGTTAAAGCA
    TACTTTTTCA TAAATGTGGC TGCAACAATA AATTGTGTCC CAGAACAGAC AATGAAACCA
    GTCGGCCATG GAGTTATGAA TGAGAAAAGC AAACCAAATC TTCCAAACTT GAAAGAGATG
    TGTTTAAAGG ATGAGGGAGG CTCACTTTGT CATCTGACAA AATTGAAAGA GAACTTTGCT
    CTTAGCACAC TAAAGTCAAT TTTACTGACA GAAGCTGAAG ACAGACTCAA CACCCTTCTG
    TCGGAGATGG ACCATCTAAG CCATAAGGAC CTCTCTCAGT GTTCTGCTGG CGAACTGGAG
    ATTGTAGTGG AGGTCAGACT GCAGCTGGCT GCCATTGCCC TGCAGAGACA CCGGGCAGCA
    TACAGTGCTG CGATAGTATT TTCCACACTT AAACTTCTCC AGGATTCAAA ACTTTTTAAA
    AAGAAAGTAG TAGAAGATGA CTCAGAGAAT TGCACCTCTT CAGAAAGTTA TGCGACTGAA
    AGTAAAGACG ACAATTTTTT AGATCCTATT TCCTTAAATT CTCGAGAATA TTTCAACATT
    CATCTGTGGT TGAGGTGCCG CTTAGCCTTG GTGACTGCAT TTGTTGCCCA GATTCGTGGC
    ATTGGAATCG TGAAAGAAAA CGATATAACA GATTACGTGA GCCTTATCAA TGAAGTGTGT
    GTGGAGGCAA AAGCTGCAGG TGACAGGGAG CTACAGGCTG AATTCTTGAT GCAAGCTGTA
    ATTATCGGCC TACAAGAAAA GCATTTAAAG GCAGACATCA TAAAAAGCCT TCAGGAGGTA
    ATACAGTTGC TTGAAGGGCA TGACTTTATT TCTCCTCGAT CTCATTTAAC CCTGGTGAGA
    AGCATGCTTT TGCTGGATGA TTTAACAAAA GCTGAGAAAT TCAAGGAAAC TCCTTTTTCG
    AAAATAGAAA AATTGTATTT GTTGACTCAG TCTCACAACA TTCTTATAGA ACAGATGATA
    ACTTTTGGAG AAACAATTGA ATTGCCTTCA TCAAACACTG ACTATGCAAG TCCATTACTG
    CCTTTGAAAA ATATTTATCT TCCTCACGTC ATGTTGTTGG CCAAAACAAA AATGAGAATT
    GGACATACAG TGGCCAAGCA AGTATATTAT ACCAGTAAAA AGAAGGATTC TTCAAAGTGG
    TTACCTGCCC TTCATTTTTT TGAAATAGCT TTGAAGCTCG CTAGGACATC AGGAACAGAA
    GAACATGAGG TGGAAGCTGA AATCTTTTTT CAGAAAGGCA AAATAGAATG TCAAATATTG
    ATGGAAGAGA AACCTCCAAC TATGCAACTT GAGAGTCTGT TTGAAGCTAT ACAGCTAAGC
    TTGAGAAATG ATCAAAATTC AGGGTTGATA AGAGACTCCT ACCTTGAAAT AGCCTTATTA
    TTTTTACACA TGAGGAAGTT AAAGAGCAAA CTTTCAGTGT CACCATTGAT GAGTATTAAG
    GCTCTTCCCA GAAGGCATAG TTCTATTAAA GAACCAGTAG TAAATAAACA TGAAATGTAC
    AGTTTATTTG CCTGGATTGC AATAAGAGCT GCTGCACAGG TCAGTGAGGC TGTGCTGGCA
    ATTAATCTAC TTATTGGAAA GAAGAATGCT AGAACTGATA CAGTTAGCCA AGGGGCATTA
    TCAAATATTC CAGAATTTGC TTCCATGGAT CTTTTGTCTT CATACACAGA TTATTTGCTT
    GATAACTACC AAGTTGTCTT CCAGACTGGT TGTACATTTT CTTACGTTAG TGAAGAGATA
    CATGAAGATG TAGATTCTCA GAAAAAAAAC CCAATTAAAG TGGATATTAC ATGGATTCTT
    CTGCTGCGCT ACTATATTCA CCTACAAAGA ATTAATAATA TGAGCAAATT GCTAGCATCT
    CTGAAGCCAG GATCTGGGAT GTCTTTGCCA GATGACACTC TTCTCACTTC TCTTTTTAAC
    TCTGGATTGA TTTTGCGCCA AAAAGAAATG CATTTTTTCC TTAAAAGATT CTTACAGCTA
    TATTCTTCTT CTTGTATTGA TGAATTTCCA AAAGAACTAT TTCAAGTCAT GGAACATCCA
    CCTGTTCTAG AAAAAGTCTT ATATGATTCA GCAAGCAAGA CAGGAGTAAC CAATGCTTCT
    CTATGCTCCG ATTTATCACT AAAGACCGTT AGCAGCACAT TGTATGGAGA CGTGGTGCCA
    CCAGTTGTGG CAACACAGGC TTTAAACAAA GAACTTTGTT TTCAGTGGTA CATTCCTCCC
    CTGGAAAAAC CTCCAAAGGA AACAGAACCT ATGGTTTTAT TGTTGTATGC ATATAATATG
    AAGCCCCTGA ATATTTCTGA TGTTGCATCT GCTGCTTGCA ACTGTGTGTA CGTAGGCTCT
    TTCTGGGTTC CACTAAAAAG AGTTATTTCA GTTCATGAGA AACTATCTAA TCTTACGCAA
    ATTGCTGAAC TATCATTGCC AACAACTCCT AAAGTTACTT CAAATGAAAA TTTACATGAA
    GTAGAGGAAA CAGAAGAGAA ATCAATTGAT GCAGAAATGG AAAACATGAT TATTGTCTGT
    TGCAATGAAA TAGTATCTTT GTTTTTGGGT GATGAAATGG CAACTCCACT ATCTGAGGTC
    CCATTTGATG TCTCGCTGCC ATCTATATTC AGTCTGGAGA GACTTTTTGA TCTTGCTAGT
    GGCTGTATCG TATCGGGAGG AAGCCTTTTC AACTGGGTGG TATCAATTAT TCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89901
    Clone ID Related Accession (Same CDS sequence) XM_015471146.1 , XM_015463359.1
    Accession Version XM_015463359.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644803.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGGCA CTTGATGATT TAATAAATAA AGGAATGCCT
    GTAATTCTGA CTACAGTCAG CCAGCAACAT CTCTTAAATA AGTTTTTAGT TGTTAAAGCA
    TACTTTTTCA TAAATGTGGC TGCAACAATA AATTGTGTCC CAGAACAGAC AATGAAACCA
    GTCGGCCATG GAGTTATGAA TGAGAAAAGC AAACCAAATC TTCCAAACTT GAAAGAGATG
    TGTTTAAAGG ATGAGGGAGG CTCACTTTGT CATCTGACAA AATTGAAAGA GAACTTTGCT
    CTTAGCACAC TAAAGTCAAT TTTACTGACA GAAGCTGAAG ACAGACTCAA CACCCTTCTG
    TCGGAGATGG ACCATCTAAG CCATAAGGAC CTCTCTCAGT GTTCTGCTGG CGAACTGGAG
    ATTGTAGTGG AGGTCAGACT GCAGCTGGCT GCCATTGCCC TGCAGAGACA CCGGGCAGCA
    TACAGTGCTG CGATAGTATT TTCCACACTT AAACTTCTCC AGGATTCAAA ACTTTTTAAA
    AAGAAAGTAG TAGAAGATGA CTCAGAGAAT TGCACCTCTT CAGAAAGTTA TGCGACTGAA
    AGTAAAGACG ACAATTTTTT AGATCCTATT TCCTTAAATT CTCGAGAATA TTTCAACATT
    CATCTGTGGT TGAGGTGCCG CTTAGCCTTG GTGACTGCAT TTGTTGCCCA GATTCGTGGC
    ATTGGAATCG TGAAAGAAAA CGATATAACA GATTACGTGA GCCTTATCAA TGAAGTGTGT
    GTGGAGGCAA AAGCTGCAGG TGACAGGGAG CTACAGGCTG AATTCTTGAT GCAAGCTGTA
    ATTATCGGCC TACAAGAAAA GCATTTAAAG GCAGACATCA TAAAAAGCCT TCAGGAGGTA
    ATACAGTTGC TTGAAGGGCA TGACTTTATT TCTCCTCGAT CTCATTTAAC CCTGGTGAGA
    AGCATGCTTT TGCTGGATGA TTTAACAAAA GCTGAGAAAT TCAAGGAAAC TCCTTTTTCG
    AAAATAGAAA AATTGTATTT GTTGACTCAG TCTCACAACA TTCTTATAGA ACAGATGATA
    ACTTTTGGAG AAACAATTGA ATTGCCTTCA TCAAACACTG ACTATGCAAG TCCATTACTG
    CCTTTGAAAA ATATTTATCT TCCTCACGTC ATGTTGTTGG CCAAAACAAA AATGAGAATT
    GGACATACAG TGGCCAAGCA AGTATATTAT ACCAGTAAAA AGAAGGATTC TTCAAAGTGG
    TTACCTGCCC TTCATTTTTT TGAAATAGCT TTGAAGCTCG CTAGGACATC AGGAACAGAA
    GAACATGAGG TGGAAGCTGA AATCTTTTTT CAGAAAGGCA AAATAGAATG TCAAATATTG
    ATGGAAGAGA AACCTCCAAC TATGCAACTT GAGAGTCTGT TTGAAGCTAT ACAGCTAAGC
    TTGAGAAATG ATCAAAATTC AGGGTTGATA AGAGACTCCT ACCTTGAAAT AGCCTTATTA
    TTTTTACACA TGAGGAAGTT AAAGAGCAAA CTTTCAGTGT CACCATTGAT GAGTATTAAG
    GCTCTTCCCA GAAGGCATAG TTCTATTAAA GAACCAGTAG TAAATAAACA TGAAATGTAC
    AGTTTATTTG CCTGGATTGC AATAAGAGCT GCTGCACAGG TCAGTGAGGC TGTGCTGGCA
    ATTAATCTAC TTATTGGAAA GAAGAATGCT AGAACTGATA CAGTTAGCCA AGGGGCATTA
    TCAAATATTC CAGAATTTGC TTCCATGGAT CTTTTGTCTT CATACACAGA TTATTTGCTT
    GATAACTACC AAGTTGTCTT CCAGACTGGT TGTACATTTT CTTACGTTAG TGAAGAGATA
    CATGAAGATG TAGATTCTCA GAAAAAAAAC CCAATTAAAG TGGATATTAC ATGGATTCTT
    CTGCTGCGCT ACTATATTCA CCTACAAAGA ATTAATAATA TGAGCAAATT GCTAGCATCT
    CTGAAGCCAG GATCTGGGAT GTCTTTGCCA GATGACACTC TTCTCACTTC TCTTTTTAAC
    TCTGGATTGA TTTTGCGCCA AAAAGAAATG CATTTTTTCC TTAAAAGATT CTTACAGCTA
    TATTCTTCTT CTTGTATTGA TGAATTTCCA AAAGAACTAT TTCAAGTCAT GGAACATCCA
    CCTGTTCTAG AAAAAGTCTT ATATGATTCA GCAAGCAAGA CAGGAGTAAC CAATGCTTCT
    CTATGCTCCG ATTTATCACT AAAGACCGTT AGCAGCACAT TGTATGGAGA CGTGGTGCCA
    CCAGTTGTGG CAACACAGGC TTTAAACAAA GAACTTTGTT TTCAGTGGTA CATTCCTCCC
    CTGGAAAAAC CTCCAAAGGA AACAGAACCT ATGGTTTTAT TGTTGTATGC ATATAATATG
    AAGCCCCTGA ATATTTCTGA TGTTGCATCT GCTGCTTGCA ACTGTGTGTA CGTAGGCTCT
    TTCTGGGTTC CACTAAAAAG AGTTATTTCA GTTCATGAGA AACTATCTAA TCTTACGCAA
    ATTGCTGAAC TATCATTGCC AACAACTCCT AAAGTTACTT CAAATGAAAA TTTACATGAA
    GTAGAGGAAA CAGAAGAGAA ATCAATTGAT GCAGAAATGG AAAACATGAT TATTGTCTGT
    TGCAATGAAA TAGTATCTTT GTTTTTGGGT GATGAAATGG CAACTCCACT ATCTGAGGTC
    CCATTTGATG TCTCGCTGCC ATCTATATTC AGTCTGGAGA GACTTTTTGA TCTTGCTAGT
    GGCTGTATCG TATCGGGAGG AAGCCTTTTC AACTGGGTGG TATCAATTAT TCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318845.1
    CDS17..9553
    Translation

    Target ORF information:

    RefSeq Version XM_015463359.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463359.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGGCA CTTGATGATT TAATAAATAA AGGAATGCCT
    GTAATTCTGA CTACAGTCAG CCAGCAACAT CTCTTAAATA AGTTTTTAGT TGTTAAAGCA
    TACTTTTTCA TAAATGTGGC TGCAACAATA AATTGTGTCC CAGAACAGAC AATGAAACCA
    GTCGGCCATG GAGTTATGAA TGAGAAAAGC AAACCAAATC TTCCAAACTT GAAAGAGATG
    TGTTTAAAGG ATGAGGGAGG CTCACTTTGT CATCTGACAA AATTGAAAGA GAACTTTGCT
    CTTAGCACAC TAAAGTCAAT TTTACTGACA GAAGCTGAAG ACAGACTCAA CACCCTTCTG
    TCGGAGATGG ACCATCTAAG CCATAAGGAC CTCTCTCAGT GTTCTGCTGG CGAACTGGAG
    ATTGTAGTGG AGGTCAGACT GCAGCTGGCT GCCATTGCCC TGCAGAGACA CCGGGCAGCA
    TACAGTGCTG CGATAGTATT TTCCACACTT AAACTTCTCC AGGATTCAAA ACTTTTTAAA
    AAGAAAGTAG TAGAAGATGA CTCAGAGAAT TGCACCTCTT CAGAAAGTTA TGCGACTGAA
    AGTAAAGACG ACAATTTTTT AGATCCTATT TCCTTAAATT CTCGAGAATA TTTCAACATT
    CATCTGTGGT TGAGGTGCCG CTTAGCCTTG GTGACTGCAT TTGTTGCCCA GATTCGTGGC
    ATTGGAATCG TGAAAGAAAA CGATATAACA GATTACGTGA GCCTTATCAA TGAAGTGTGT
    GTGGAGGCAA AAGCTGCAGG TGACAGGGAG CTACAGGCTG AATTCTTGAT GCAAGCTGTA
    ATTATCGGCC TACAAGAAAA GCATTTAAAG GCAGACATCA TAAAAAGCCT TCAGGAGGTA
    ATACAGTTGC TTGAAGGGCA TGACTTTATT TCTCCTCGAT CTCATTTAAC CCTGGTGAGA
    AGCATGCTTT TGCTGGATGA TTTAACAAAA GCTGAGAAAT TCAAGGAAAC TCCTTTTTCG
    AAAATAGAAA AATTGTATTT GTTGACTCAG TCTCACAACA TTCTTATAGA ACAGATGATA
    ACTTTTGGAG AAACAATTGA ATTGCCTTCA TCAAACACTG ACTATGCAAG TCCATTACTG
    CCTTTGAAAA ATATTTATCT TCCTCACGTC ATGTTGTTGG CCAAAACAAA AATGAGAATT
    GGACATACAG TGGCCAAGCA AGTATATTAT ACCAGTAAAA AGAAGGATTC TTCAAAGTGG
    TTACCTGCCC TTCATTTTTT TGAAATAGCT TTGAAGCTCG CTAGGACATC AGGAACAGAA
    GAACATGAGG TGGAAGCTGA AATCTTTTTT CAGAAAGGCA AAATAGAATG TCAAATATTG
    ATGGAAGAGA AACCTCCAAC TATGCAACTT GAGAGTCTGT TTGAAGCTAT ACAGCTAAGC
    TTGAGAAATG ATCAAAATTC AGGGTTGATA AGAGACTCCT ACCTTGAAAT AGCCTTATTA
    TTTTTACACA TGAGGAAGTT AAAGAGCAAA CTTTCAGTGT CACCATTGAT GAGTATTAAG
    GCTCTTCCCA GAAGGCATAG TTCTATTAAA GAACCAGTAG TAAATAAACA TGAAATGTAC
    AGTTTATTTG CCTGGATTGC AATAAGAGCT GCTGCACAGG TCAGTGAGGC TGTGCTGGCA
    ATTAATCTAC TTATTGGAAA GAAGAATGCT AGAACTGATA CAGTTAGCCA AGGGGCATTA
    TCAAATATTC CAGAATTTGC TTCCATGGAT CTTTTGTCTT CATACACAGA TTATTTGCTT
    GATAACTACC AAGTTGTCTT CCAGACTGGT TGTACATTTT CTTACGTTAG TGAAGAGATA
    CATGAAGATG TAGATTCTCA GAAAAAAAAC CCAATTAAAG TGGATATTAC ATGGATTCTT
    CTGCTGCGCT ACTATATTCA CCTACAAAGA ATTAATAATA TGAGCAAATT GCTAGCATCT
    CTGAAGCCAG GATCTGGGAT GTCTTTGCCA GATGACACTC TTCTCACTTC TCTTTTTAAC
    TCTGGATTGA TTTTGCGCCA AAAAGAAATG CATTTTTTCC TTAAAAGATT CTTACAGCTA
    TATTCTTCTT CTTGTATTGA TGAATTTCCA AAAGAACTAT TTCAAGTCAT GGAACATCCA
    CCTGTTCTAG AAAAAGTCTT ATATGATTCA GCAAGCAAGA CAGGAGTAAC CAATGCTTCT
    CTATGCTCCG ATTTATCACT AAAGACCGTT AGCAGCACAT TGTATGGAGA CGTGGTGCCA
    CCAGTTGTGG CAACACAGGC TTTAAACAAA GAACTTTGTT TTCAGTGGTA CATTCCTCCC
    CTGGAAAAAC CTCCAAAGGA AACAGAACCT ATGGTTTTAT TGTTGTATGC ATATAATATG
    AAGCCCCTGA ATATTTCTGA TGTTGCATCT GCTGCTTGCA ACTGTGTGTA CGTAGGCTCT
    TTCTGGGTTC CACTAAAAAG AGTTATTTCA GTTCATGAGA AACTATCTAA TCTTACGCAA
    ATTGCTGAAC TATCATTGCC AACAACTCCT AAAGTTACTT CAAATGAAAA TTTACATGAA
    GTAGAGGAAA CAGAAGAGAA ATCAATTGAT GCAGAAATGG AAAACATGAT TATTGTCTGT
    TGCAATGAAA TAGTATCTTT GTTTTTGGGT GATGAAATGG CAACTCCACT ATCTGAGGTC
    CCATTTGATG TCTCGCTGCC ATCTATATTC AGTCTGGAGA GACTTTTTGA TCTTGCTAGT
    GGCTGTATCG TATCGGGAGG AAGCCTTTTC AACTGGGTGG TATCAATTAT TCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89902
    Clone ID Related Accession (Same CDS sequence) XM_015463360.1 , XM_015471147.1
    Accession Version XM_015463360.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9516bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644803.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGACAGGAG TAACCAATGC TTCTCTATGC
    TCCGATTTAT CACTAAAGAC CGTTAGCAGC ACATTGTATG GAGACGTGGT GCCACCAGTT
    GTGGCAACAC AGGCTTTAAA CAAAGAACTT TGTTTTCAGT GGTACATTCC TCCCCTGGAA
    AAACCTCCAA AGGAAACAGA ACCTATGGTT TTATTGTTGT ATGCATATAA TATGAAGCCC
    CTGAATATTT CTGATGTTGC ATCTGCTGCT TGCAACTGTG TGTACGTAGG CTCTTTCTGG
    GTTCCACTAA AAAGAGTTAT TTCAGTTCAT GAGAAACTAT CTAATCTTAC GCAAATTGCT
    GAACTATCAT TGCCAACAAC TCCTAAAGTT ACTTCAAATG AAAATTTACA TGAAGTAGAG
    GAAACAGAAG AGAAATCAAT TGATGCAGAA ATGGAAGTCC CATTTGATGT CTCGCTGCCA
    TCTATATTCA GTCTGGAGAG ACTTTTTGAT CTTGCTAGTG GCTGTATCGT ATCGGGAGGA
    AGCCTTTTCA ACTGGGTGGT ATCAATTATT CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318846.1
    CDS19..9534
    Translation

    Target ORF information:

    RefSeq Version XM_015463360.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463360.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGACAGGAG TAACCAATGC TTCTCTATGC
    TCCGATTTAT CACTAAAGAC CGTTAGCAGC ACATTGTATG GAGACGTGGT GCCACCAGTT
    GTGGCAACAC AGGCTTTAAA CAAAGAACTT TGTTTTCAGT GGTACATTCC TCCCCTGGAA
    AAACCTCCAA AGGAAACAGA ACCTATGGTT TTATTGTTGT ATGCATATAA TATGAAGCCC
    CTGAATATTT CTGATGTTGC ATCTGCTGCT TGCAACTGTG TGTACGTAGG CTCTTTCTGG
    GTTCCACTAA AAAGAGTTAT TTCAGTTCAT GAGAAACTAT CTAATCTTAC GCAAATTGCT
    GAACTATCAT TGCCAACAAC TCCTAAAGTT ACTTCAAATG AAAATTTACA TGAAGTAGAG
    GAAACAGAAG AGAAATCAAT TGATGCAGAA ATGGAAGTCC CATTTGATGT CTCGCTGCCA
    TCTATATTCA GTCTGGAGAG ACTTTTTGAT CTTGCTAGTG GCTGTATCGT ATCGGGAGGA
    AGCCTTTTCA ACTGGGTGGT ATCAATTATT CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89902
    Clone ID Related Accession (Same CDS sequence) XM_015463360.1 , XM_015471147.1
    Accession Version XM_015471147.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9516bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGACAGGAG TAACCAATGC TTCTCTATGC
    TCCGATTTAT CACTAAAGAC CGTTAGCAGC ACATTGTATG GAGACGTGGT GCCACCAGTT
    GTGGCAACAC AGGCTTTAAA CAAAGAACTT TGTTTTCAGT GGTACATTCC TCCCCTGGAA
    AAACCTCCAA AGGAAACAGA ACCTATGGTT TTATTGTTGT ATGCATATAA TATGAAGCCC
    CTGAATATTT CTGATGTTGC ATCTGCTGCT TGCAACTGTG TGTACGTAGG CTCTTTCTGG
    GTTCCACTAA AAAGAGTTAT TTCAGTTCAT GAGAAACTAT CTAATCTTAC GCAAATTGCT
    GAACTATCAT TGCCAACAAC TCCTAAAGTT ACTTCAAATG AAAATTTACA TGAAGTAGAG
    GAAACAGAAG AGAAATCAAT TGATGCAGAA ATGGAAGTCC CATTTGATGT CTCGCTGCCA
    TCTATATTCA GTCTGGAGAG ACTTTTTGAT CTTGCTAGTG GCTGTATCGT ATCGGGAGGA
    AGCCTTTTCA ACTGGGTGGT ATCAATTATT CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326633.1
    CDS19..9534
    Translation

    Target ORF information:

    RefSeq Version XM_015471147.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471147.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGACAGGAG TAACCAATGC TTCTCTATGC
    TCCGATTTAT CACTAAAGAC CGTTAGCAGC ACATTGTATG GAGACGTGGT GCCACCAGTT
    GTGGCAACAC AGGCTTTAAA CAAAGAACTT TGTTTTCAGT GGTACATTCC TCCCCTGGAA
    AAACCTCCAA AGGAAACAGA ACCTATGGTT TTATTGTTGT ATGCATATAA TATGAAGCCC
    CTGAATATTT CTGATGTTGC ATCTGCTGCT TGCAACTGTG TGTACGTAGG CTCTTTCTGG
    GTTCCACTAA AAAGAGTTAT TTCAGTTCAT GAGAAACTAT CTAATCTTAC GCAAATTGCT
    GAACTATCAT TGCCAACAAC TCCTAAAGTT ACTTCAAATG AAAATTTACA TGAAGTAGAG
    GAAACAGAAG AGAAATCAAT TGATGCAGAA ATGGAAGTCC CATTTGATGT CTCGCTGCCA
    TCTATATTCA GTCTGGAGAG ACTTTTTGAT CTTGCTAGTG GCTGTATCGT ATCGGGAGGA
    AGCCTTTTCA ACTGGGTGGT ATCAATTATT CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89903
    Clone ID Related Accession (Same CDS sequence) XM_015471148.1 , XM_015463361.1
    Accession Version XM_015471148.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9417bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGGTTTTAT TGTTGTATGC ATATAATATG
    AAGCCCCTGA ATATTTCTGA TGTTGCATCT GCTGCTTGCA ACTGTGTGTA CGTAGGCTCT
    TTCTGGGTTC CACTAAAAAG AGTTATTTCA GTTCATGAGA AACTATCTAA TCTTACGCAA
    ATTGCTGAAC TATCATTGCC AACAACTCCT AAAGTTACTT CAAATGAAAA TTTACATGAA
    GTAGAGGAAA CAGAAGAGAA ATCAATTGAT GCAGAAATGG AAAACATGAT TATTGTCTGT
    TGCAATGAAA TAGTATCTTT GTTTTTGGGT GATGAAATGG CAACTCCACT ATCTGAGGTC
    CCATTTGATG TCTCGCTGCC ATCTATATTC AGTCTGGAGA GACTTTTTGA TCTTGCTAGT
    GGCTGTATCG TATCGGGAGG AAGCCTTTTC AACTGGGTGG TATCAATTAT TCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326634.1
    CDS20..9436
    Translation

    Target ORF information:

    RefSeq Version XM_015471148.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471148.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGGTTTTAT TGTTGTATGC ATATAATATG
    AAGCCCCTGA ATATTTCTGA TGTTGCATCT GCTGCTTGCA ACTGTGTGTA CGTAGGCTCT
    TTCTGGGTTC CACTAAAAAG AGTTATTTCA GTTCATGAGA AACTATCTAA TCTTACGCAA
    ATTGCTGAAC TATCATTGCC AACAACTCCT AAAGTTACTT CAAATGAAAA TTTACATGAA
    GTAGAGGAAA CAGAAGAGAA ATCAATTGAT GCAGAAATGG AAAACATGAT TATTGTCTGT
    TGCAATGAAA TAGTATCTTT GTTTTTGGGT GATGAAATGG CAACTCCACT ATCTGAGGTC
    CCATTTGATG TCTCGCTGCC ATCTATATTC AGTCTGGAGA GACTTTTTGA TCTTGCTAGT
    GGCTGTATCG TATCGGGAGG AAGCCTTTTC AACTGGGTGG TATCAATTAT TCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89903
    Clone ID Related Accession (Same CDS sequence) XM_015471148.1 , XM_015463361.1
    Accession Version XM_015463361.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9417bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644803.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGGTTTTAT TGTTGTATGC ATATAATATG
    AAGCCCCTGA ATATTTCTGA TGTTGCATCT GCTGCTTGCA ACTGTGTGTA CGTAGGCTCT
    TTCTGGGTTC CACTAAAAAG AGTTATTTCA GTTCATGAGA AACTATCTAA TCTTACGCAA
    ATTGCTGAAC TATCATTGCC AACAACTCCT AAAGTTACTT CAAATGAAAA TTTACATGAA
    GTAGAGGAAA CAGAAGAGAA ATCAATTGAT GCAGAAATGG AAAACATGAT TATTGTCTGT
    TGCAATGAAA TAGTATCTTT GTTTTTGGGT GATGAAATGG CAACTCCACT ATCTGAGGTC
    CCATTTGATG TCTCGCTGCC ATCTATATTC AGTCTGGAGA GACTTTTTGA TCTTGCTAGT
    GGCTGTATCG TATCGGGAGG AAGCCTTTTC AACTGGGTGG TATCAATTAT TCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318847.1
    CDS20..9436
    Translation

    Target ORF information:

    RefSeq Version XM_015463361.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463361.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGGTTTTAT TGTTGTATGC ATATAATATG
    AAGCCCCTGA ATATTTCTGA TGTTGCATCT GCTGCTTGCA ACTGTGTGTA CGTAGGCTCT
    TTCTGGGTTC CACTAAAAAG AGTTATTTCA GTTCATGAGA AACTATCTAA TCTTACGCAA
    ATTGCTGAAC TATCATTGCC AACAACTCCT AAAGTTACTT CAAATGAAAA TTTACATGAA
    GTAGAGGAAA CAGAAGAGAA ATCAATTGAT GCAGAAATGG AAAACATGAT TATTGTCTGT
    TGCAATGAAA TAGTATCTTT GTTTTTGGGT GATGAAATGG CAACTCCACT ATCTGAGGTC
    CCATTTGATG TCTCGCTGCC ATCTATATTC AGTCTGGAGA GACTTTTTGA TCTTGCTAGT
    GGCTGTATCG TATCGGGAGG AAGCCTTTTC AACTGGGTGG TATCAATTAT TCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89904
    Clone ID Related Accession (Same CDS sequence) XM_015471149.1 , XM_015463362.1
    Accession Version XM_015471149.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGACAGGAG TAACCAATGC TTCTCTATGC
    TCCGATTTAT CACTAAAGAC CGTTAGCAGC ACATTGTATG GAGACGTGGT GCCACCAGTT
    GTGGCAACAC AGGCTTTAAA CAAAGAACTT TGTTTTCAGT GGTACATTCC TCCCCTGGAA
    AAACCTCCAA AGGAAACAGA ACCTATGGTT TTATTGTTGT ATGCATATAA TATGAAGCCC
    CTGAATATTT CTGATGTTGC ATCTGCTGCT TGCAACTGTG TGTACGTAGG CTCTTTCTGG
    GTTCCACTAA AAAGAGTTAT TTCAGTTCAT GAGAAACTAT CTAATCTTAC GCAAATTGCT
    GAACTATCAT TGCCAACAAC TCCTAAAGTT ACTTCAAATG AAAATTTACA TGAAGTAGAG
    GAAACAGAAG AGAAATCAAT TGATGCAGAA ATGGAAAACA TGATTATTGT CTGTTGCAAT
    GAAATAGTAT CTTTGTTTTT GGGTGATGAA ATGGCAACTC CACTATCTGA GGTCCCATTT
    GATGTCTCGC TGCCATCTAT ATTCAGTCTG GAGAGACTTT TTGATCTTGC TAGTGGCTGT
    ATCGTATCGG GAGGAAGCCT TTTCAACTGG GTGGTATCAA TTATTCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326635.1
    CDS19..9429
    Translation

    Target ORF information:

    RefSeq Version XM_015471149.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471149.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGACAGGAG TAACCAATGC TTCTCTATGC
    TCCGATTTAT CACTAAAGAC CGTTAGCAGC ACATTGTATG GAGACGTGGT GCCACCAGTT
    GTGGCAACAC AGGCTTTAAA CAAAGAACTT TGTTTTCAGT GGTACATTCC TCCCCTGGAA
    AAACCTCCAA AGGAAACAGA ACCTATGGTT TTATTGTTGT ATGCATATAA TATGAAGCCC
    CTGAATATTT CTGATGTTGC ATCTGCTGCT TGCAACTGTG TGTACGTAGG CTCTTTCTGG
    GTTCCACTAA AAAGAGTTAT TTCAGTTCAT GAGAAACTAT CTAATCTTAC GCAAATTGCT
    GAACTATCAT TGCCAACAAC TCCTAAAGTT ACTTCAAATG AAAATTTACA TGAAGTAGAG
    GAAACAGAAG AGAAATCAAT TGATGCAGAA ATGGAAAACA TGATTATTGT CTGTTGCAAT
    GAAATAGTAT CTTTGTTTTT GGGTGATGAA ATGGCAACTC CACTATCTGA GGTCCCATTT
    GATGTCTCGC TGCCATCTAT ATTCAGTCTG GAGAGACTTT TTGATCTTGC TAGTGGCTGT
    ATCGTATCGG GAGGAAGCCT TTTCAACTGG GTGGTATCAA TTATTCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89904
    Clone ID Related Accession (Same CDS sequence) XM_015471149.1 , XM_015463362.1
    Accession Version XM_015463362.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644803.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGACAGGAG TAACCAATGC TTCTCTATGC
    TCCGATTTAT CACTAAAGAC CGTTAGCAGC ACATTGTATG GAGACGTGGT GCCACCAGTT
    GTGGCAACAC AGGCTTTAAA CAAAGAACTT TGTTTTCAGT GGTACATTCC TCCCCTGGAA
    AAACCTCCAA AGGAAACAGA ACCTATGGTT TTATTGTTGT ATGCATATAA TATGAAGCCC
    CTGAATATTT CTGATGTTGC ATCTGCTGCT TGCAACTGTG TGTACGTAGG CTCTTTCTGG
    GTTCCACTAA AAAGAGTTAT TTCAGTTCAT GAGAAACTAT CTAATCTTAC GCAAATTGCT
    GAACTATCAT TGCCAACAAC TCCTAAAGTT ACTTCAAATG AAAATTTACA TGAAGTAGAG
    GAAACAGAAG AGAAATCAAT TGATGCAGAA ATGGAAAACA TGATTATTGT CTGTTGCAAT
    GAAATAGTAT CTTTGTTTTT GGGTGATGAA ATGGCAACTC CACTATCTGA GGTCCCATTT
    GATGTCTCGC TGCCATCTAT ATTCAGTCTG GAGAGACTTT TTGATCTTGC TAGTGGCTGT
    ATCGTATCGG GAGGAAGCCT TTTCAACTGG GTGGTATCAA TTATTCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318848.1
    CDS19..9429
    Translation

    Target ORF information:

    RefSeq Version XM_015463362.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463362.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGGCTCTT
    CCCAGAAGGC ATAGTTCTAT TAAAGAACCA GTAGTAAATA AACATGAAAT GTACAGTTTA
    TTTGCCTGGA TTGCAATAAG AGCTGCTGCA CAGGTCAGTG AGGCTGTGCT GGCAATTAAT
    CTACTTATTG GAAAGAAGAA TGCTAGAACT GATACAGTTA GCCAAGGGGC ATTATCAAAT
    ATTCCAGAAT TTGCTTCCAT GGATCTTTTG TCTTCATACA CAGATTATTT GCTTGATAAC
    TACCAAGTTG TCTTCCAGAC TGGTTGTACA TTTTCTTACG TTAGTGAAGA GATACATGAA
    GATGTAGATT CTCAGAAAAA AAACCCAATT AAAGTGGATA TTACATGGAT TCTTCTGCTG
    CGCTACTATA TTCACCTACA AAGAATTAAT AATATGAGCA AATTGCTAGC ATCTCTGAAG
    CCAGGATCTG GGATGTCTTT GCCAGATGAC ACTCTTCTCA CTTCTCTTTT TAACTCTGGA
    TTGATTTTGC GCCAAAAAGA AATGCATTTT TTCCTTAAAA GATTCTTACA GCTATATTCT
    TCTTCTTGTA TTGATGAATT TCCAAAAGAA CTATTTCAAG TCATGGAACA TCCACCTGTT
    CTAGAAAAAG TCTTATATGA TTCAGCAAGC AAGACAGGAG TAACCAATGC TTCTCTATGC
    TCCGATTTAT CACTAAAGAC CGTTAGCAGC ACATTGTATG GAGACGTGGT GCCACCAGTT
    GTGGCAACAC AGGCTTTAAA CAAAGAACTT TGTTTTCAGT GGTACATTCC TCCCCTGGAA
    AAACCTCCAA AGGAAACAGA ACCTATGGTT TTATTGTTGT ATGCATATAA TATGAAGCCC
    CTGAATATTT CTGATGTTGC ATCTGCTGCT TGCAACTGTG TGTACGTAGG CTCTTTCTGG
    GTTCCACTAA AAAGAGTTAT TTCAGTTCAT GAGAAACTAT CTAATCTTAC GCAAATTGCT
    GAACTATCAT TGCCAACAAC TCCTAAAGTT ACTTCAAATG AAAATTTACA TGAAGTAGAG
    GAAACAGAAG AGAAATCAAT TGATGCAGAA ATGGAAAACA TGATTATTGT CTGTTGCAAT
    GAAATAGTAT CTTTGTTTTT GGGTGATGAA ATGGCAACTC CACTATCTGA GGTCCCATTT
    GATGTCTCGC TGCCATCTAT ATTCAGTCTG GAGAGACTTT TTGATCTTGC TAGTGGCTGT
    ATCGTATCGG GAGGAAGCCT TTTCAACTGG GTGGTATCAA TTATTCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89905
    Clone ID Related Accession (Same CDS sequence) XM_015463363.1 , XM_015471150.1
    Accession Version XM_015463363.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9396bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644803.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGATGTTC TTCAAACCAG CAATCAAAGA
    AGTCTTCATG TGCAGGCGTT GCATGAACTT GGAAATCTTC TCATCTTCGC ACAGAAGAAA
    AGGGCTGCTT TTAAGTGTTG GAGTCAAGCT CTGGATGAAA TTTTCAGAAA AGAAGATGTG
    CTCCATACAT GGAAGGAGAT TGGCAGCTCA CTCACCAGTG CCACTGATGG CTGGTCACCT
    CCAGGTTCCA AAGACTACAG TGAGGAGCTC CTGTCCAAAG GCGGCATCTG GGGGTGTTTG
    CAGGGAGCGG TGATATCAGC AAAGATAGCA CAATTTATTC AGACATTGGA TGTTGAAAAG
    AGAACCAATT GTTGCCTTTT ATCAGCATTA CTCTTTCAGG GTTTGCTTAG AACCACACTG
    CCGCATCCTA AAGCTGAACG TTGCTACGCT CAGTATGAAA TCACTCAGCT TCTCCCGGGC
    ATCGAGCTCT TCTCAGACAG ATATCGGGCT GACATCTGCT CCGTCATTGC GAGTTTATAT
    TACGTCATCC GCGAGCTGCA CTTTGCTAAG CAAAATCTAG TAATTCTGCC TCTCCTTGCA
    TTGTACCAAT ATTTTGTTTC TGTAATTTGC CAAGACCTAG TCAAAAATCT GGAAGCAAGA
    ATCCTCAAGA TAGAAGTCCT TACAGATTTA GGATTCTTTT CCGAAGCCTT TCATGAGCTA
    TCCCAGATAT TTTATGCGAA AAACACACCT TCTTCAGTAC CCACCAGCTG TAAACCTACT
    GGAAAAATGA AGCTATTTCA ATCATTTGAC TCGGGAAAAC CTCTTATTAG TAAAGAGAAT
    CTGCAGGCAC TTGATGATTT AATAAATAAA GGAATGCCTG TAATTCTGAC TACAGTCAGC
    CAGCAACATC TCTTAAATAA GTTTTTAGTT GTTAAAGCAT ACTTTTTCAT AAATGTGGCT
    GCAACAATAA ATTGTGTCCC AGAACAGACA ATGAAACCAG TCGGCCATGG AGTTATGAAT
    GAGAAAAGCA AACCAAATCT TCCAAACTTG AAAGAGATGT GTTTAAAGGA TGAGGGAGGC
    TCACTTTGTC ATCTGACAAA ATTGAAAGAG AACTTTGCTC TTAGCACACT AAAGTCAATT
    TTACTGACAG AAGCTGAAGA CAGACTCAAC ACCCTTCTGT CGGAGATGGA CCATCTAAGC
    CATAAGGACC TCTCTCAGTG TTCTGCTGGC GAACTGGAGA TTGTAGTGGA GGTCAGACTG
    CAGCTGGCTG CCATTGCCCT GCAGAGACAC CGGGCAGCAT ACAGTGCTGC GATAGTATTT
    TCCACACTTA AACTTCTCCA GGATTCAAAA CTTTTTAAAA AGAAAGTAGT AGAAGATGAC
    TCAGAGAATT GCACCTCTTC AGAAAGTTAT GCGACTGAAA GTAAAGACGA CAATTTTTTA
    GATCCTATTT CCTTAAATTC TCGAGAATAT TTCAACATTC ATCTGTGGTT GAGGTGCCGC
    TTAGCCTTGG TGACTGCATT TGTTGCCCAG ATTCGTGGCA TTGGAATCGT GAAAGAAAAC
    GATATAACAG ATTACGTGAG CCTTATCAAT GAAGTGTGTG TGGAGGCAAA AGCTGCAGGT
    GACAGGGAGC TACAGGCTGA ATTCTTGATG CAAGCTGTAA TTATCGGCCT ACAAGAAAAG
    CATTTAAAGG CAGACATCAT AAAAAGCCTT CAGGAGGTAA TACAGTTGCT TGAAGGGCAT
    GACTTTATTT CTCCTCGATC TCATTTAACC CTGGTGAGAA GCATGCTTTT GCTGGATGAT
    TTAACAAAAG CTGAGAAATT CAAGGAAACT CCTTTTTCGA AAATAGAAAA ATTGTATTTG
    TTGACTCAGT CTCACAACAT TCTTATAGAA CAGATGATAA CTTTTGGAGA AACAATTGAA
    TTGCCTTCAT CAAACACTGA CTATGCAAGT CCATTACTGC CTTTGAAAAA TATTTATCTT
    CCTCACGTCA TGTTGTTGGC CAAAACAAAA ATGAGAATTG GACATACAGT GGCCAAGCAA
    GTATATTATA CCAGTAAAAA GAAGGATTCT TCAAAGTGGT TACCTGCCCT TCATTTTTTT
    GAAATAGCTT TGAAGCTCGC TAGGACATCA GGAACAGAAG AACATGAGGT GGAAGCTGAA
    ATCTTTTTTC AGAAAGGCAA AATAGAATGT CAAATATTGA TGGAAGAGAA ACCTCCAACT
    ATGCAACTTG AGAGTCTGTT TGAAGCTATA CAGCTAAGCT TGAGAAATGA TCAAAATTCA
    GGGTTGATAA GAGACTCCTA CCTTGAAATA GCCTTATTAT TTTTACACAT GAGGAAGTTA
    AAGAGCAAAC TTTCAGTGTC ACCATTGATG AGTATTAAGG CTCTTCCCAG AAGGCATAGT
    TCTATTAAAG AACCAGTAGT AAATAAACAT GAAATGTACA GTTTATTTGC CTGGATTGCA
    ATAAGAGCTG CTGCACAGGT CAGTGAGGCT GTGCTGGCAA TTAATCTACT TATTGGAAAG
    AAGAATGCTA GAACTGATAC AGTTAGCCAA GGGGCATTAT CAAATATTCC AGAATTTGCT
    TCCATGGATC TTTTGTCTTC ATACACAGAT TATTTGCTTG ATAACTACCA AGTTGTCTTC
    CAGACTGGTT GTACATTTTC TTACGTTAGT GAAGAGATAC ATGAAGATGT AGATTCTCAG
    AAAAAAAACC CAATTAAAGT GGATATTACA TGGATTCTTC TGCTGCGCTA CTATATTCAC
    CTACAAAGAA TTAATAATAT GAGCAAATTG CTAGCATCTC TGAAGCCAGG ATCTGGGATG
    TCTTTGCCAG ATGACACTCT TCTCACTTCT CTTTTTAACT CTGGATTGAT TTTGCGCCAA
    AAAGAAATGC ATTTTTTCCT TAAAAGATTC TTACAGCTAT ATTCTTCTTC TTGTATTGAT
    GAATTTCCAA AAGAACTATT TCAAGTCATG GAACATCCAC CTGTTCTAGA AAAAGTCTTA
    TATGATTCAG CAAGCAAGAC AGGAGTAACC AATGCTTCTC TATGCTCCGA TTTATCACTA
    AAGACCGTTA GCAGCACATT GTATGGAGAC GTGGTGCCAC CAGTTGTGGC AACACAGGCT
    TTAAACAAAG AACTTTGTTT TCAGTGGTAC ATTCCTCCCC TGGAAAAACC TCCAAAGGAA
    ACAGAACCTA TGGTTTTATT GTTGTATGCA TATAATATGA AGCCCCTGAA TATTTCTGAT
    GTTGCATCTG CTGCTTGCAA CTGTGTGTAC GTAGGCTCTT TCTGGGTTCC ACTAAAAAGA
    GTTATTTCAG TTCATGAGAA ACTATCTAAT CTTACGCAAA TTGCTGAACT ATCATTGCCA
    ACAACTCCTA AAGTTACTTC AAATGAAAAT TTACATGAAG TAGAGGAAAC AGAAGAGAAA
    TCAATTGATG CAGAAATGGA AAACATGATT ATTGTCTGTT GCAATGAAAT AGTATCTTTG
    TTTTTGGGTG ATGAAATGGC AACTCCACTA TCTGAGGTCC CATTTGATGT CTCGCTGCCA
    TCTATATTCA GTCTGGAGAG ACTTTTTGAT CTTGCTAGTG GCTGTATCGT ATCGGGAGGA
    AGCCTTTTCA ACTGGGTGGT ATCAATTATT CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318849.1
    CDS17..9412
    Translation

    Target ORF information:

    RefSeq Version XM_015463363.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463363.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGATGTTC TTCAAACCAG CAATCAAAGA
    AGTCTTCATG TGCAGGCGTT GCATGAACTT GGAAATCTTC TCATCTTCGC ACAGAAGAAA
    AGGGCTGCTT TTAAGTGTTG GAGTCAAGCT CTGGATGAAA TTTTCAGAAA AGAAGATGTG
    CTCCATACAT GGAAGGAGAT TGGCAGCTCA CTCACCAGTG CCACTGATGG CTGGTCACCT
    CCAGGTTCCA AAGACTACAG TGAGGAGCTC CTGTCCAAAG GCGGCATCTG GGGGTGTTTG
    CAGGGAGCGG TGATATCAGC AAAGATAGCA CAATTTATTC AGACATTGGA TGTTGAAAAG
    AGAACCAATT GTTGCCTTTT ATCAGCATTA CTCTTTCAGG GTTTGCTTAG AACCACACTG
    CCGCATCCTA AAGCTGAACG TTGCTACGCT CAGTATGAAA TCACTCAGCT TCTCCCGGGC
    ATCGAGCTCT TCTCAGACAG ATATCGGGCT GACATCTGCT CCGTCATTGC GAGTTTATAT
    TACGTCATCC GCGAGCTGCA CTTTGCTAAG CAAAATCTAG TAATTCTGCC TCTCCTTGCA
    TTGTACCAAT ATTTTGTTTC TGTAATTTGC CAAGACCTAG TCAAAAATCT GGAAGCAAGA
    ATCCTCAAGA TAGAAGTCCT TACAGATTTA GGATTCTTTT CCGAAGCCTT TCATGAGCTA
    TCCCAGATAT TTTATGCGAA AAACACACCT TCTTCAGTAC CCACCAGCTG TAAACCTACT
    GGAAAAATGA AGCTATTTCA ATCATTTGAC TCGGGAAAAC CTCTTATTAG TAAAGAGAAT
    CTGCAGGCAC TTGATGATTT AATAAATAAA GGAATGCCTG TAATTCTGAC TACAGTCAGC
    CAGCAACATC TCTTAAATAA GTTTTTAGTT GTTAAAGCAT ACTTTTTCAT AAATGTGGCT
    GCAACAATAA ATTGTGTCCC AGAACAGACA ATGAAACCAG TCGGCCATGG AGTTATGAAT
    GAGAAAAGCA AACCAAATCT TCCAAACTTG AAAGAGATGT GTTTAAAGGA TGAGGGAGGC
    TCACTTTGTC ATCTGACAAA ATTGAAAGAG AACTTTGCTC TTAGCACACT AAAGTCAATT
    TTACTGACAG AAGCTGAAGA CAGACTCAAC ACCCTTCTGT CGGAGATGGA CCATCTAAGC
    CATAAGGACC TCTCTCAGTG TTCTGCTGGC GAACTGGAGA TTGTAGTGGA GGTCAGACTG
    CAGCTGGCTG CCATTGCCCT GCAGAGACAC CGGGCAGCAT ACAGTGCTGC GATAGTATTT
    TCCACACTTA AACTTCTCCA GGATTCAAAA CTTTTTAAAA AGAAAGTAGT AGAAGATGAC
    TCAGAGAATT GCACCTCTTC AGAAAGTTAT GCGACTGAAA GTAAAGACGA CAATTTTTTA
    GATCCTATTT CCTTAAATTC TCGAGAATAT TTCAACATTC ATCTGTGGTT GAGGTGCCGC
    TTAGCCTTGG TGACTGCATT TGTTGCCCAG ATTCGTGGCA TTGGAATCGT GAAAGAAAAC
    GATATAACAG ATTACGTGAG CCTTATCAAT GAAGTGTGTG TGGAGGCAAA AGCTGCAGGT
    GACAGGGAGC TACAGGCTGA ATTCTTGATG CAAGCTGTAA TTATCGGCCT ACAAGAAAAG
    CATTTAAAGG CAGACATCAT AAAAAGCCTT CAGGAGGTAA TACAGTTGCT TGAAGGGCAT
    GACTTTATTT CTCCTCGATC TCATTTAACC CTGGTGAGAA GCATGCTTTT GCTGGATGAT
    TTAACAAAAG CTGAGAAATT CAAGGAAACT CCTTTTTCGA AAATAGAAAA ATTGTATTTG
    TTGACTCAGT CTCACAACAT TCTTATAGAA CAGATGATAA CTTTTGGAGA AACAATTGAA
    TTGCCTTCAT CAAACACTGA CTATGCAAGT CCATTACTGC CTTTGAAAAA TATTTATCTT
    CCTCACGTCA TGTTGTTGGC CAAAACAAAA ATGAGAATTG GACATACAGT GGCCAAGCAA
    GTATATTATA CCAGTAAAAA GAAGGATTCT TCAAAGTGGT TACCTGCCCT TCATTTTTTT
    GAAATAGCTT TGAAGCTCGC TAGGACATCA GGAACAGAAG AACATGAGGT GGAAGCTGAA
    ATCTTTTTTC AGAAAGGCAA AATAGAATGT CAAATATTGA TGGAAGAGAA ACCTCCAACT
    ATGCAACTTG AGAGTCTGTT TGAAGCTATA CAGCTAAGCT TGAGAAATGA TCAAAATTCA
    GGGTTGATAA GAGACTCCTA CCTTGAAATA GCCTTATTAT TTTTACACAT GAGGAAGTTA
    AAGAGCAAAC TTTCAGTGTC ACCATTGATG AGTATTAAGG CTCTTCCCAG AAGGCATAGT
    TCTATTAAAG AACCAGTAGT AAATAAACAT GAAATGTACA GTTTATTTGC CTGGATTGCA
    ATAAGAGCTG CTGCACAGGT CAGTGAGGCT GTGCTGGCAA TTAATCTACT TATTGGAAAG
    AAGAATGCTA GAACTGATAC AGTTAGCCAA GGGGCATTAT CAAATATTCC AGAATTTGCT
    TCCATGGATC TTTTGTCTTC ATACACAGAT TATTTGCTTG ATAACTACCA AGTTGTCTTC
    CAGACTGGTT GTACATTTTC TTACGTTAGT GAAGAGATAC ATGAAGATGT AGATTCTCAG
    AAAAAAAACC CAATTAAAGT GGATATTACA TGGATTCTTC TGCTGCGCTA CTATATTCAC
    CTACAAAGAA TTAATAATAT GAGCAAATTG CTAGCATCTC TGAAGCCAGG ATCTGGGATG
    TCTTTGCCAG ATGACACTCT TCTCACTTCT CTTTTTAACT CTGGATTGAT TTTGCGCCAA
    AAAGAAATGC ATTTTTTCCT TAAAAGATTC TTACAGCTAT ATTCTTCTTC TTGTATTGAT
    GAATTTCCAA AAGAACTATT TCAAGTCATG GAACATCCAC CTGTTCTAGA AAAAGTCTTA
    TATGATTCAG CAAGCAAGAC AGGAGTAACC AATGCTTCTC TATGCTCCGA TTTATCACTA
    AAGACCGTTA GCAGCACATT GTATGGAGAC GTGGTGCCAC CAGTTGTGGC AACACAGGCT
    TTAAACAAAG AACTTTGTTT TCAGTGGTAC ATTCCTCCCC TGGAAAAACC TCCAAAGGAA
    ACAGAACCTA TGGTTTTATT GTTGTATGCA TATAATATGA AGCCCCTGAA TATTTCTGAT
    GTTGCATCTG CTGCTTGCAA CTGTGTGTAC GTAGGCTCTT TCTGGGTTCC ACTAAAAAGA
    GTTATTTCAG TTCATGAGAA ACTATCTAAT CTTACGCAAA TTGCTGAACT ATCATTGCCA
    ACAACTCCTA AAGTTACTTC AAATGAAAAT TTACATGAAG TAGAGGAAAC AGAAGAGAAA
    TCAATTGATG CAGAAATGGA AAACATGATT ATTGTCTGTT GCAATGAAAT AGTATCTTTG
    TTTTTGGGTG ATGAAATGGC AACTCCACTA TCTGAGGTCC CATTTGATGT CTCGCTGCCA
    TCTATATTCA GTCTGGAGAG ACTTTTTGAT CTTGCTAGTG GCTGTATCGT ATCGGGAGGA
    AGCCTTTTCA ACTGGGTGGT ATCAATTATT CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89905
    Clone ID Related Accession (Same CDS sequence) XM_015463363.1 , XM_015471150.1
    Accession Version XM_015471150.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9396bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGATGTTC TTCAAACCAG CAATCAAAGA
    AGTCTTCATG TGCAGGCGTT GCATGAACTT GGAAATCTTC TCATCTTCGC ACAGAAGAAA
    AGGGCTGCTT TTAAGTGTTG GAGTCAAGCT CTGGATGAAA TTTTCAGAAA AGAAGATGTG
    CTCCATACAT GGAAGGAGAT TGGCAGCTCA CTCACCAGTG CCACTGATGG CTGGTCACCT
    CCAGGTTCCA AAGACTACAG TGAGGAGCTC CTGTCCAAAG GCGGCATCTG GGGGTGTTTG
    CAGGGAGCGG TGATATCAGC AAAGATAGCA CAATTTATTC AGACATTGGA TGTTGAAAAG
    AGAACCAATT GTTGCCTTTT ATCAGCATTA CTCTTTCAGG GTTTGCTTAG AACCACACTG
    CCGCATCCTA AAGCTGAACG TTGCTACGCT CAGTATGAAA TCACTCAGCT TCTCCCGGGC
    ATCGAGCTCT TCTCAGACAG ATATCGGGCT GACATCTGCT CCGTCATTGC GAGTTTATAT
    TACGTCATCC GCGAGCTGCA CTTTGCTAAG CAAAATCTAG TAATTCTGCC TCTCCTTGCA
    TTGTACCAAT ATTTTGTTTC TGTAATTTGC CAAGACCTAG TCAAAAATCT GGAAGCAAGA
    ATCCTCAAGA TAGAAGTCCT TACAGATTTA GGATTCTTTT CCGAAGCCTT TCATGAGCTA
    TCCCAGATAT TTTATGCGAA AAACACACCT TCTTCAGTAC CCACCAGCTG TAAACCTACT
    GGAAAAATGA AGCTATTTCA ATCATTTGAC TCGGGAAAAC CTCTTATTAG TAAAGAGAAT
    CTGCAGGCAC TTGATGATTT AATAAATAAA GGAATGCCTG TAATTCTGAC TACAGTCAGC
    CAGCAACATC TCTTAAATAA GTTTTTAGTT GTTAAAGCAT ACTTTTTCAT AAATGTGGCT
    GCAACAATAA ATTGTGTCCC AGAACAGACA ATGAAACCAG TCGGCCATGG AGTTATGAAT
    GAGAAAAGCA AACCAAATCT TCCAAACTTG AAAGAGATGT GTTTAAAGGA TGAGGGAGGC
    TCACTTTGTC ATCTGACAAA ATTGAAAGAG AACTTTGCTC TTAGCACACT AAAGTCAATT
    TTACTGACAG AAGCTGAAGA CAGACTCAAC ACCCTTCTGT CGGAGATGGA CCATCTAAGC
    CATAAGGACC TCTCTCAGTG TTCTGCTGGC GAACTGGAGA TTGTAGTGGA GGTCAGACTG
    CAGCTGGCTG CCATTGCCCT GCAGAGACAC CGGGCAGCAT ACAGTGCTGC GATAGTATTT
    TCCACACTTA AACTTCTCCA GGATTCAAAA CTTTTTAAAA AGAAAGTAGT AGAAGATGAC
    TCAGAGAATT GCACCTCTTC AGAAAGTTAT GCGACTGAAA GTAAAGACGA CAATTTTTTA
    GATCCTATTT CCTTAAATTC TCGAGAATAT TTCAACATTC ATCTGTGGTT GAGGTGCCGC
    TTAGCCTTGG TGACTGCATT TGTTGCCCAG ATTCGTGGCA TTGGAATCGT GAAAGAAAAC
    GATATAACAG ATTACGTGAG CCTTATCAAT GAAGTGTGTG TGGAGGCAAA AGCTGCAGGT
    GACAGGGAGC TACAGGCTGA ATTCTTGATG CAAGCTGTAA TTATCGGCCT ACAAGAAAAG
    CATTTAAAGG CAGACATCAT AAAAAGCCTT CAGGAGGTAA TACAGTTGCT TGAAGGGCAT
    GACTTTATTT CTCCTCGATC TCATTTAACC CTGGTGAGAA GCATGCTTTT GCTGGATGAT
    TTAACAAAAG CTGAGAAATT CAAGGAAACT CCTTTTTCGA AAATAGAAAA ATTGTATTTG
    TTGACTCAGT CTCACAACAT TCTTATAGAA CAGATGATAA CTTTTGGAGA AACAATTGAA
    TTGCCTTCAT CAAACACTGA CTATGCAAGT CCATTACTGC CTTTGAAAAA TATTTATCTT
    CCTCACGTCA TGTTGTTGGC CAAAACAAAA ATGAGAATTG GACATACAGT GGCCAAGCAA
    GTATATTATA CCAGTAAAAA GAAGGATTCT TCAAAGTGGT TACCTGCCCT TCATTTTTTT
    GAAATAGCTT TGAAGCTCGC TAGGACATCA GGAACAGAAG AACATGAGGT GGAAGCTGAA
    ATCTTTTTTC AGAAAGGCAA AATAGAATGT CAAATATTGA TGGAAGAGAA ACCTCCAACT
    ATGCAACTTG AGAGTCTGTT TGAAGCTATA CAGCTAAGCT TGAGAAATGA TCAAAATTCA
    GGGTTGATAA GAGACTCCTA CCTTGAAATA GCCTTATTAT TTTTACACAT GAGGAAGTTA
    AAGAGCAAAC TTTCAGTGTC ACCATTGATG AGTATTAAGG CTCTTCCCAG AAGGCATAGT
    TCTATTAAAG AACCAGTAGT AAATAAACAT GAAATGTACA GTTTATTTGC CTGGATTGCA
    ATAAGAGCTG CTGCACAGGT CAGTGAGGCT GTGCTGGCAA TTAATCTACT TATTGGAAAG
    AAGAATGCTA GAACTGATAC AGTTAGCCAA GGGGCATTAT CAAATATTCC AGAATTTGCT
    TCCATGGATC TTTTGTCTTC ATACACAGAT TATTTGCTTG ATAACTACCA AGTTGTCTTC
    CAGACTGGTT GTACATTTTC TTACGTTAGT GAAGAGATAC ATGAAGATGT AGATTCTCAG
    AAAAAAAACC CAATTAAAGT GGATATTACA TGGATTCTTC TGCTGCGCTA CTATATTCAC
    CTACAAAGAA TTAATAATAT GAGCAAATTG CTAGCATCTC TGAAGCCAGG ATCTGGGATG
    TCTTTGCCAG ATGACACTCT TCTCACTTCT CTTTTTAACT CTGGATTGAT TTTGCGCCAA
    AAAGAAATGC ATTTTTTCCT TAAAAGATTC TTACAGCTAT ATTCTTCTTC TTGTATTGAT
    GAATTTCCAA AAGAACTATT TCAAGTCATG GAACATCCAC CTGTTCTAGA AAAAGTCTTA
    TATGATTCAG CAAGCAAGAC AGGAGTAACC AATGCTTCTC TATGCTCCGA TTTATCACTA
    AAGACCGTTA GCAGCACATT GTATGGAGAC GTGGTGCCAC CAGTTGTGGC AACACAGGCT
    TTAAACAAAG AACTTTGTTT TCAGTGGTAC ATTCCTCCCC TGGAAAAACC TCCAAAGGAA
    ACAGAACCTA TGGTTTTATT GTTGTATGCA TATAATATGA AGCCCCTGAA TATTTCTGAT
    GTTGCATCTG CTGCTTGCAA CTGTGTGTAC GTAGGCTCTT TCTGGGTTCC ACTAAAAAGA
    GTTATTTCAG TTCATGAGAA ACTATCTAAT CTTACGCAAA TTGCTGAACT ATCATTGCCA
    ACAACTCCTA AAGTTACTTC AAATGAAAAT TTACATGAAG TAGAGGAAAC AGAAGAGAAA
    TCAATTGATG CAGAAATGGA AAACATGATT ATTGTCTGTT GCAATGAAAT AGTATCTTTG
    TTTTTGGGTG ATGAAATGGC AACTCCACTA TCTGAGGTCC CATTTGATGT CTCGCTGCCA
    TCTATATTCA GTCTGGAGAG ACTTTTTGAT CTTGCTAGTG GCTGTATCGT ATCGGGAGGA
    AGCCTTTTCA ACTGGGTGGT ATCAATTATT CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326636.1
    CDS17..9412
    Translation

    Target ORF information:

    RefSeq Version XM_015471150.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471150.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGATGTTC TTCAAACCAG CAATCAAAGA
    AGTCTTCATG TGCAGGCGTT GCATGAACTT GGAAATCTTC TCATCTTCGC ACAGAAGAAA
    AGGGCTGCTT TTAAGTGTTG GAGTCAAGCT CTGGATGAAA TTTTCAGAAA AGAAGATGTG
    CTCCATACAT GGAAGGAGAT TGGCAGCTCA CTCACCAGTG CCACTGATGG CTGGTCACCT
    CCAGGTTCCA AAGACTACAG TGAGGAGCTC CTGTCCAAAG GCGGCATCTG GGGGTGTTTG
    CAGGGAGCGG TGATATCAGC AAAGATAGCA CAATTTATTC AGACATTGGA TGTTGAAAAG
    AGAACCAATT GTTGCCTTTT ATCAGCATTA CTCTTTCAGG GTTTGCTTAG AACCACACTG
    CCGCATCCTA AAGCTGAACG TTGCTACGCT CAGTATGAAA TCACTCAGCT TCTCCCGGGC
    ATCGAGCTCT TCTCAGACAG ATATCGGGCT GACATCTGCT CCGTCATTGC GAGTTTATAT
    TACGTCATCC GCGAGCTGCA CTTTGCTAAG CAAAATCTAG TAATTCTGCC TCTCCTTGCA
    TTGTACCAAT ATTTTGTTTC TGTAATTTGC CAAGACCTAG TCAAAAATCT GGAAGCAAGA
    ATCCTCAAGA TAGAAGTCCT TACAGATTTA GGATTCTTTT CCGAAGCCTT TCATGAGCTA
    TCCCAGATAT TTTATGCGAA AAACACACCT TCTTCAGTAC CCACCAGCTG TAAACCTACT
    GGAAAAATGA AGCTATTTCA ATCATTTGAC TCGGGAAAAC CTCTTATTAG TAAAGAGAAT
    CTGCAGGCAC TTGATGATTT AATAAATAAA GGAATGCCTG TAATTCTGAC TACAGTCAGC
    CAGCAACATC TCTTAAATAA GTTTTTAGTT GTTAAAGCAT ACTTTTTCAT AAATGTGGCT
    GCAACAATAA ATTGTGTCCC AGAACAGACA ATGAAACCAG TCGGCCATGG AGTTATGAAT
    GAGAAAAGCA AACCAAATCT TCCAAACTTG AAAGAGATGT GTTTAAAGGA TGAGGGAGGC
    TCACTTTGTC ATCTGACAAA ATTGAAAGAG AACTTTGCTC TTAGCACACT AAAGTCAATT
    TTACTGACAG AAGCTGAAGA CAGACTCAAC ACCCTTCTGT CGGAGATGGA CCATCTAAGC
    CATAAGGACC TCTCTCAGTG TTCTGCTGGC GAACTGGAGA TTGTAGTGGA GGTCAGACTG
    CAGCTGGCTG CCATTGCCCT GCAGAGACAC CGGGCAGCAT ACAGTGCTGC GATAGTATTT
    TCCACACTTA AACTTCTCCA GGATTCAAAA CTTTTTAAAA AGAAAGTAGT AGAAGATGAC
    TCAGAGAATT GCACCTCTTC AGAAAGTTAT GCGACTGAAA GTAAAGACGA CAATTTTTTA
    GATCCTATTT CCTTAAATTC TCGAGAATAT TTCAACATTC ATCTGTGGTT GAGGTGCCGC
    TTAGCCTTGG TGACTGCATT TGTTGCCCAG ATTCGTGGCA TTGGAATCGT GAAAGAAAAC
    GATATAACAG ATTACGTGAG CCTTATCAAT GAAGTGTGTG TGGAGGCAAA AGCTGCAGGT
    GACAGGGAGC TACAGGCTGA ATTCTTGATG CAAGCTGTAA TTATCGGCCT ACAAGAAAAG
    CATTTAAAGG CAGACATCAT AAAAAGCCTT CAGGAGGTAA TACAGTTGCT TGAAGGGCAT
    GACTTTATTT CTCCTCGATC TCATTTAACC CTGGTGAGAA GCATGCTTTT GCTGGATGAT
    TTAACAAAAG CTGAGAAATT CAAGGAAACT CCTTTTTCGA AAATAGAAAA ATTGTATTTG
    TTGACTCAGT CTCACAACAT TCTTATAGAA CAGATGATAA CTTTTGGAGA AACAATTGAA
    TTGCCTTCAT CAAACACTGA CTATGCAAGT CCATTACTGC CTTTGAAAAA TATTTATCTT
    CCTCACGTCA TGTTGTTGGC CAAAACAAAA ATGAGAATTG GACATACAGT GGCCAAGCAA
    GTATATTATA CCAGTAAAAA GAAGGATTCT TCAAAGTGGT TACCTGCCCT TCATTTTTTT
    GAAATAGCTT TGAAGCTCGC TAGGACATCA GGAACAGAAG AACATGAGGT GGAAGCTGAA
    ATCTTTTTTC AGAAAGGCAA AATAGAATGT CAAATATTGA TGGAAGAGAA ACCTCCAACT
    ATGCAACTTG AGAGTCTGTT TGAAGCTATA CAGCTAAGCT TGAGAAATGA TCAAAATTCA
    GGGTTGATAA GAGACTCCTA CCTTGAAATA GCCTTATTAT TTTTACACAT GAGGAAGTTA
    AAGAGCAAAC TTTCAGTGTC ACCATTGATG AGTATTAAGG CTCTTCCCAG AAGGCATAGT
    TCTATTAAAG AACCAGTAGT AAATAAACAT GAAATGTACA GTTTATTTGC CTGGATTGCA
    ATAAGAGCTG CTGCACAGGT CAGTGAGGCT GTGCTGGCAA TTAATCTACT TATTGGAAAG
    AAGAATGCTA GAACTGATAC AGTTAGCCAA GGGGCATTAT CAAATATTCC AGAATTTGCT
    TCCATGGATC TTTTGTCTTC ATACACAGAT TATTTGCTTG ATAACTACCA AGTTGTCTTC
    CAGACTGGTT GTACATTTTC TTACGTTAGT GAAGAGATAC ATGAAGATGT AGATTCTCAG
    AAAAAAAACC CAATTAAAGT GGATATTACA TGGATTCTTC TGCTGCGCTA CTATATTCAC
    CTACAAAGAA TTAATAATAT GAGCAAATTG CTAGCATCTC TGAAGCCAGG ATCTGGGATG
    TCTTTGCCAG ATGACACTCT TCTCACTTCT CTTTTTAACT CTGGATTGAT TTTGCGCCAA
    AAAGAAATGC ATTTTTTCCT TAAAAGATTC TTACAGCTAT ATTCTTCTTC TTGTATTGAT
    GAATTTCCAA AAGAACTATT TCAAGTCATG GAACATCCAC CTGTTCTAGA AAAAGTCTTA
    TATGATTCAG CAAGCAAGAC AGGAGTAACC AATGCTTCTC TATGCTCCGA TTTATCACTA
    AAGACCGTTA GCAGCACATT GTATGGAGAC GTGGTGCCAC CAGTTGTGGC AACACAGGCT
    TTAAACAAAG AACTTTGTTT TCAGTGGTAC ATTCCTCCCC TGGAAAAACC TCCAAAGGAA
    ACAGAACCTA TGGTTTTATT GTTGTATGCA TATAATATGA AGCCCCTGAA TATTTCTGAT
    GTTGCATCTG CTGCTTGCAA CTGTGTGTAC GTAGGCTCTT TCTGGGTTCC ACTAAAAAGA
    GTTATTTCAG TTCATGAGAA ACTATCTAAT CTTACGCAAA TTGCTGAACT ATCATTGCCA
    ACAACTCCTA AAGTTACTTC AAATGAAAAT TTACATGAAG TAGAGGAAAC AGAAGAGAAA
    TCAATTGATG CAGAAATGGA AAACATGATT ATTGTCTGTT GCAATGAAAT AGTATCTTTG
    TTTTTGGGTG ATGAAATGGC AACTCCACTA TCTGAGGTCC CATTTGATGT CTCGCTGCCA
    TCTATATTCA GTCTGGAGAG ACTTTTTGAT CTTGCTAGTG GCTGTATCGT ATCGGGAGGA
    AGCCTTTTCA ACTGGGTGGT ATCAATTATT CCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89906
    Clone ID Related Accession (Same CDS sequence) XM_010827774.2 , XM_010805261.3 , XM_010805261.2
    Accession Version XM_010827774.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644803.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010827774.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
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    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GATGTTCTTC AAACCAGCAA TCAAAGAAGT
    CTTCATGTGC AGGCGTTGCA TGAACTTGGA AATCTTCTCA TCTTCGCACA GAAGAAAAGG
    GCTGCTTTTA AGTGTTGGAG TCAAGCTCTG GATGAAATTT TCAGAAAAGA AGATGTGCTC
    CATACATGGA AGGAGATTGG CAGCTCACTC ACCAGTGCCA CTGATGGCTG GTCACCTCCA
    GGTTCCAAAG ACTACAGTGA GGAGCTCCTG TCCAAAGGCG GCATCTGGGG GTGTTTGCAG
    GGAGCGGTGA TATCAGCAAA GATAGCACAA TTTATTCAGA CATTGGATGT TGAAAAGAGA
    ACCAATTGTT GCCTTTTATC AGCATTACTC TTTCAGGGTT TGCTTAGAAC CACACTGCCG
    CATCCTAAAG CTGAACGTTG CTACGCTCAG TATGAAATCA CTCAGCTTCT CCCGGGCATC
    GAGCTCTTCT CAGACAGATA TCGGGCTGAC ATCTGCTCCG TCATTGCGAG TTTATATTAC
    GTCATCCGCG AGCTGCACTT TGCTAAGCAA AATCTAGTAA TTCTGCCTCT CCTTGCATTG
    TACCAATATT TTGTTTCTGT AATTTGCCAA GACCTAGTCA AAAATCTGGA AGCAAGAATC
    CTCAAGATAG AAGTCCTTAC AGATTTAGGA TTCTTTTCCG AAGCCTTTCA TGAGCTATCC
    CAGATATTTT ATGCGAAAAA CACACCTTCT TCAGTACCCA CCAGCTGTAA ACCTACTGGA
    AAAATGAAGC TATTTCAATC ATTTGACTCG GGAAAACCTC TTATTAGTAA AGAGAATCTG
    CAGGCACTTG ATGATTTAAT AAATAAAGGA ATGCCTGTAA TTCTGACTAC AGTCAGCCAG
    CAACATCTCT TAAATAAGTT TTTAGTTGTT AAAGCATACT TTTTCATAAA TGTGGCTGCA
    ACAATAAATT GTGTCCCAGA ACAGACAATG AAACCAGTCG GCCATGGAGT TATGAATGAG
    AAAAGCAAAC CAAATCTTCC AAACTTGAAA GAGATGTGTT TAAAGGATGA GGGAGGCTCA
    CTTTGTCATC TGACAAAATT GAAAGAGAAC TTTGCTCTTA GCACACTAAA GTCAATTTTA
    CTGACAGAAG CTGAAGACAG ACTCAACACC CTTCTGTCGG AGATGGACCA TCTAAGCCAT
    AAGGACCTCT CTCAGTGTTC TGCTGGCGAA CTGGAGATTG TAGTGGAGGT CAGACTGCAG
    CTGGCTGCCA TTGCCCTGCA GAGACACCGG GCAGCATACA GTGCTGCGAT AGTATTTTCC
    ACACTTAAAC TTCTCCAGGA TTCAAAACTT TTTAAAAAGA AAGTAGTAGA AGATGACTCA
    GAGAATTGCA CCTCTTCAGA AAGTTATGCG ACTGAAAGTA AAGACGACAA TTTTTTAGAT
    CCTATTTCCT TAAATTCTCG AGAATATTTC AACATTCATC TGTGGTTGAG GTGCCGCTTA
    GCCTTGGTGA CTGCATTTGT TGCCCAGATT CGTGGCATTG GAATCGTGAA AGAAAACGAT
    ATAACAGATT ACGTGAGCCT TATCAATGAA GTGTGTGTGG AGGCAAAAGC TGCAGGTGAC
    AGGGAGCTAC AGGCTGAATT CTTGATGCAA GCTGTAATTA TCGGCCTACA AGAAAAGCAT
    TTAAAGGCAG ACATCATAAA AAGCCTTCAG GAGGTAATAC AGTTGCTTGA AGGGCATGAC
    TTTATTTCTC CTCGATCTCA TTTAACCCTG GTGAGAAGCA TGCTTTTGCT GGATGATTTA
    ACAAAAGCTG AGAAATTCAA GGAAACTCCT TTTTCGAAAA TAGAAAAATT GTATTTGTTG
    ACTCAGTCTC ACAACATTCT TATAGAACAG ATGATAACTT TTGGAGAAAC AATTGAATTG
    CCTTCATCAA ACACTGACTA TGCAAGTCCA TTACTGCCTT TGAAAAATAT TTATCTTCCT
    CACGTCATGT TGTTGGCCAA AACAAAAATG AGAATTGGAC ATACAGTGGC CAAGCAAGTA
    TATTATACCA GTAAAAAGAA GGATTCTTCA AAGTGGTTAC CTGCCCTTCA TTTTTTTGAA
    ATAGCTTTGA AGCTCGCTAG GACATCAGGA ACAGAAGAAC ATGAGGTGGA AGCTGAAATC
    TTTTTTCAGA AAGGCAAAAT AGAATGTCAA ATATTGATGG AAGAGAAACC TCCAACTATG
    CAACTTGAGA GTCTGTTTGA AGCTATACAG CTAAGCTTGA GAAATGATCA AAATTCAGGG
    TTGATAAGAG ACTCCTACCT TGAAATAGCC TTATTATTTT TACACATGAG GAAGTTAAAG
    AGCAAACTTT CAGTGTCACC ATTGATGAGT ATTAAGGCTC TTCCCAGAAG GCATAGTTCT
    ATTAAAGAAC CAGTAGTAAA TAAACATGAA ATGTACAGTT TATTTGCCTG GATTGCAATA
    AGAGCTGCTG CACAGGTCAG TGAGGCTGTG CTGGCAATTA ATCTACTTAT TGGAAAGAAG
    AATGCTAGAA CTGATACAGT TAGCCAAGGG GCATTATCAA ATATTCCAGA ATTTGCTTCC
    ATGGATCTTT TGTCTTCATA CACAGATTAT TTGCTTGATA ACTACCAAGT TGTCTTCCAG
    ACTGGTTGTA CATTTTCTTA CGTTAGTGAA GAGATACATG AAGATGTAGA TTCTCAGAAA
    AAAAACCCAA TTAAAGTGGA TATTACATGG ATTCTTCTGC TGCGCTACTA TATTCACCTA
    CAAAGAATTA ATAATATGAG CAAATTGCTA GCATCTCTGA AGCCAGGATC TGGGATGTCT
    TTGCCAGATG ACACTCTTCT CACTTCTCTT TTTAACTCTG GATTGATTTT GCGCCAAAAA
    GAAATGCATT TTTTCCTTAA AAGATTCTTA CAGCTATATT CTTCTTCTTG TATTGATGAA
    TTTCCAAAAG AACTATTTCA AGTCATGGAA CATCCACCTG TTCTAGAAAA AGTCTTATAT
    GATTCAGCAA GCAAGACAGG AGTAACCAAT GCTTCTCTAT GCTCCGATTT ATCACTAAAG
    ACCGTTAGCA GCACATTGTA TGGAGACGTG GTGCCACCAG TTGTGGCAAC ACAGGCTTTA
    AACAAAGAAC TTTGTTTTCA GTGGTACATT CCTCCCCTGG AAAAACCTCC AAAGGAAACA
    GAACCTATGG TTTTATTGTT GTATGCATAT AATATGAAGC CCCTGAATAT TTCTGATGTT
    GCATCTGCTG CTTGCAACTG TGTGTACGTA GGCTCTTTCT GGGTTCCACT AAAAAGAGTT
    ATTTCAGTTC ATGAGAAACT ATCTAATCTT ACGCAAATTG CTGAACTATC ATTGCCAACA
    ACTCCTAAAG TTACTTCAAA TGAAAATTTA CATGAAGTAG AGGAAACAGA AGAGAAATCA
    ATTGATGCAG AAATGGAAAA CATGATTATT GTCTGTTGCA ATGAAATAGT ATCTTTGTTT
    TTGGGTGATG AAATGGCAAC TCCACTATCT GAGGTCCCAT TTGATGTCTC GCTGCCATCT
    ATATTCAGTC TGGAGAGACT TTTTGATCTT GCTAGTGGCT GTATCGTATC GGGAGGAAGC
    CTTTTCAACT GGGTGGTATC AATTATTCCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010826076.2
    CDS16..9408
    Translation

    Target ORF information:

    RefSeq Version XM_010827774.2
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010827774.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GATGTTCTTC AAACCAGCAA TCAAAGAAGT
    CTTCATGTGC AGGCGTTGCA TGAACTTGGA AATCTTCTCA TCTTCGCACA GAAGAAAAGG
    GCTGCTTTTA AGTGTTGGAG TCAAGCTCTG GATGAAATTT TCAGAAAAGA AGATGTGCTC
    CATACATGGA AGGAGATTGG CAGCTCACTC ACCAGTGCCA CTGATGGCTG GTCACCTCCA
    GGTTCCAAAG ACTACAGTGA GGAGCTCCTG TCCAAAGGCG GCATCTGGGG GTGTTTGCAG
    GGAGCGGTGA TATCAGCAAA GATAGCACAA TTTATTCAGA CATTGGATGT TGAAAAGAGA
    ACCAATTGTT GCCTTTTATC AGCATTACTC TTTCAGGGTT TGCTTAGAAC CACACTGCCG
    CATCCTAAAG CTGAACGTTG CTACGCTCAG TATGAAATCA CTCAGCTTCT CCCGGGCATC
    GAGCTCTTCT CAGACAGATA TCGGGCTGAC ATCTGCTCCG TCATTGCGAG TTTATATTAC
    GTCATCCGCG AGCTGCACTT TGCTAAGCAA AATCTAGTAA TTCTGCCTCT CCTTGCATTG
    TACCAATATT TTGTTTCTGT AATTTGCCAA GACCTAGTCA AAAATCTGGA AGCAAGAATC
    CTCAAGATAG AAGTCCTTAC AGATTTAGGA TTCTTTTCCG AAGCCTTTCA TGAGCTATCC
    CAGATATTTT ATGCGAAAAA CACACCTTCT TCAGTACCCA CCAGCTGTAA ACCTACTGGA
    AAAATGAAGC TATTTCAATC ATTTGACTCG GGAAAACCTC TTATTAGTAA AGAGAATCTG
    CAGGCACTTG ATGATTTAAT AAATAAAGGA ATGCCTGTAA TTCTGACTAC AGTCAGCCAG
    CAACATCTCT TAAATAAGTT TTTAGTTGTT AAAGCATACT TTTTCATAAA TGTGGCTGCA
    ACAATAAATT GTGTCCCAGA ACAGACAATG AAACCAGTCG GCCATGGAGT TATGAATGAG
    AAAAGCAAAC CAAATCTTCC AAACTTGAAA GAGATGTGTT TAAAGGATGA GGGAGGCTCA
    CTTTGTCATC TGACAAAATT GAAAGAGAAC TTTGCTCTTA GCACACTAAA GTCAATTTTA
    CTGACAGAAG CTGAAGACAG ACTCAACACC CTTCTGTCGG AGATGGACCA TCTAAGCCAT
    AAGGACCTCT CTCAGTGTTC TGCTGGCGAA CTGGAGATTG TAGTGGAGGT CAGACTGCAG
    CTGGCTGCCA TTGCCCTGCA GAGACACCGG GCAGCATACA GTGCTGCGAT AGTATTTTCC
    ACACTTAAAC TTCTCCAGGA TTCAAAACTT TTTAAAAAGA AAGTAGTAGA AGATGACTCA
    GAGAATTGCA CCTCTTCAGA AAGTTATGCG ACTGAAAGTA AAGACGACAA TTTTTTAGAT
    CCTATTTCCT TAAATTCTCG AGAATATTTC AACATTCATC TGTGGTTGAG GTGCCGCTTA
    GCCTTGGTGA CTGCATTTGT TGCCCAGATT CGTGGCATTG GAATCGTGAA AGAAAACGAT
    ATAACAGATT ACGTGAGCCT TATCAATGAA GTGTGTGTGG AGGCAAAAGC TGCAGGTGAC
    AGGGAGCTAC AGGCTGAATT CTTGATGCAA GCTGTAATTA TCGGCCTACA AGAAAAGCAT
    TTAAAGGCAG ACATCATAAA AAGCCTTCAG GAGGTAATAC AGTTGCTTGA AGGGCATGAC
    TTTATTTCTC CTCGATCTCA TTTAACCCTG GTGAGAAGCA TGCTTTTGCT GGATGATTTA
    ACAAAAGCTG AGAAATTCAA GGAAACTCCT TTTTCGAAAA TAGAAAAATT GTATTTGTTG
    ACTCAGTCTC ACAACATTCT TATAGAACAG ATGATAACTT TTGGAGAAAC AATTGAATTG
    CCTTCATCAA ACACTGACTA TGCAAGTCCA TTACTGCCTT TGAAAAATAT TTATCTTCCT
    CACGTCATGT TGTTGGCCAA AACAAAAATG AGAATTGGAC ATACAGTGGC CAAGCAAGTA
    TATTATACCA GTAAAAAGAA GGATTCTTCA AAGTGGTTAC CTGCCCTTCA TTTTTTTGAA
    ATAGCTTTGA AGCTCGCTAG GACATCAGGA ACAGAAGAAC ATGAGGTGGA AGCTGAAATC
    TTTTTTCAGA AAGGCAAAAT AGAATGTCAA ATATTGATGG AAGAGAAACC TCCAACTATG
    CAACTTGAGA GTCTGTTTGA AGCTATACAG CTAAGCTTGA GAAATGATCA AAATTCAGGG
    TTGATAAGAG ACTCCTACCT TGAAATAGCC TTATTATTTT TACACATGAG GAAGTTAAAG
    AGCAAACTTT CAGTGTCACC ATTGATGAGT ATTAAGGCTC TTCCCAGAAG GCATAGTTCT
    ATTAAAGAAC CAGTAGTAAA TAAACATGAA ATGTACAGTT TATTTGCCTG GATTGCAATA
    AGAGCTGCTG CACAGGTCAG TGAGGCTGTG CTGGCAATTA ATCTACTTAT TGGAAAGAAG
    AATGCTAGAA CTGATACAGT TAGCCAAGGG GCATTATCAA ATATTCCAGA ATTTGCTTCC
    ATGGATCTTT TGTCTTCATA CACAGATTAT TTGCTTGATA ACTACCAAGT TGTCTTCCAG
    ACTGGTTGTA CATTTTCTTA CGTTAGTGAA GAGATACATG AAGATGTAGA TTCTCAGAAA
    AAAAACCCAA TTAAAGTGGA TATTACATGG ATTCTTCTGC TGCGCTACTA TATTCACCTA
    CAAAGAATTA ATAATATGAG CAAATTGCTA GCATCTCTGA AGCCAGGATC TGGGATGTCT
    TTGCCAGATG ACACTCTTCT CACTTCTCTT TTTAACTCTG GATTGATTTT GCGCCAAAAA
    GAAATGCATT TTTTCCTTAA AAGATTCTTA CAGCTATATT CTTCTTCTTG TATTGATGAA
    TTTCCAAAAG AACTATTTCA AGTCATGGAA CATCCACCTG TTCTAGAAAA AGTCTTATAT
    GATTCAGCAA GCAAGACAGG AGTAACCAAT GCTTCTCTAT GCTCCGATTT ATCACTAAAG
    ACCGTTAGCA GCACATTGTA TGGAGACGTG GTGCCACCAG TTGTGGCAAC ACAGGCTTTA
    AACAAAGAAC TTTGTTTTCA GTGGTACATT CCTCCCCTGG AAAAACCTCC AAAGGAAACA
    GAACCTATGG TTTTATTGTT GTATGCATAT AATATGAAGC CCCTGAATAT TTCTGATGTT
    GCATCTGCTG CTTGCAACTG TGTGTACGTA GGCTCTTTCT GGGTTCCACT AAAAAGAGTT
    ATTTCAGTTC ATGAGAAACT ATCTAATCTT ACGCAAATTG CTGAACTATC ATTGCCAACA
    ACTCCTAAAG TTACTTCAAA TGAAAATTTA CATGAAGTAG AGGAAACAGA AGAGAAATCA
    ATTGATGCAG AAATGGAAAA CATGATTATT GTCTGTTGCA ATGAAATAGT ATCTTTGTTT
    TTGGGTGATG AAATGGCAAC TCCACTATCT GAGGTCCCAT TTGATGTCTC GCTGCCATCT
    ATATTCAGTC TGGAGAGACT TTTTGATCTT GCTAGTGGCT GTATCGTATC GGGAGGAAGC
    CTTTTCAACT GGGTGGTATC AATTATTCCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89906
    Clone ID Related Accession (Same CDS sequence) XM_010827774.2 , XM_010805261.3 , XM_010805261.2
    Accession Version XM_010805261.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805261.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GATGTTCTTC AAACCAGCAA TCAAAGAAGT
    CTTCATGTGC AGGCGTTGCA TGAACTTGGA AATCTTCTCA TCTTCGCACA GAAGAAAAGG
    GCTGCTTTTA AGTGTTGGAG TCAAGCTCTG GATGAAATTT TCAGAAAAGA AGATGTGCTC
    CATACATGGA AGGAGATTGG CAGCTCACTC ACCAGTGCCA CTGATGGCTG GTCACCTCCA
    GGTTCCAAAG ACTACAGTGA GGAGCTCCTG TCCAAAGGCG GCATCTGGGG GTGTTTGCAG
    GGAGCGGTGA TATCAGCAAA GATAGCACAA TTTATTCAGA CATTGGATGT TGAAAAGAGA
    ACCAATTGTT GCCTTTTATC AGCATTACTC TTTCAGGGTT TGCTTAGAAC CACACTGCCG
    CATCCTAAAG CTGAACGTTG CTACGCTCAG TATGAAATCA CTCAGCTTCT CCCGGGCATC
    GAGCTCTTCT CAGACAGATA TCGGGCTGAC ATCTGCTCCG TCATTGCGAG TTTATATTAC
    GTCATCCGCG AGCTGCACTT TGCTAAGCAA AATCTAGTAA TTCTGCCTCT CCTTGCATTG
    TACCAATATT TTGTTTCTGT AATTTGCCAA GACCTAGTCA AAAATCTGGA AGCAAGAATC
    CTCAAGATAG AAGTCCTTAC AGATTTAGGA TTCTTTTCCG AAGCCTTTCA TGAGCTATCC
    CAGATATTTT ATGCGAAAAA CACACCTTCT TCAGTACCCA CCAGCTGTAA ACCTACTGGA
    AAAATGAAGC TATTTCAATC ATTTGACTCG GGAAAACCTC TTATTAGTAA AGAGAATCTG
    CAGGCACTTG ATGATTTAAT AAATAAAGGA ATGCCTGTAA TTCTGACTAC AGTCAGCCAG
    CAACATCTCT TAAATAAGTT TTTAGTTGTT AAAGCATACT TTTTCATAAA TGTGGCTGCA
    ACAATAAATT GTGTCCCAGA ACAGACAATG AAACCAGTCG GCCATGGAGT TATGAATGAG
    AAAAGCAAAC CAAATCTTCC AAACTTGAAA GAGATGTGTT TAAAGGATGA GGGAGGCTCA
    CTTTGTCATC TGACAAAATT GAAAGAGAAC TTTGCTCTTA GCACACTAAA GTCAATTTTA
    CTGACAGAAG CTGAAGACAG ACTCAACACC CTTCTGTCGG AGATGGACCA TCTAAGCCAT
    AAGGACCTCT CTCAGTGTTC TGCTGGCGAA CTGGAGATTG TAGTGGAGGT CAGACTGCAG
    CTGGCTGCCA TTGCCCTGCA GAGACACCGG GCAGCATACA GTGCTGCGAT AGTATTTTCC
    ACACTTAAAC TTCTCCAGGA TTCAAAACTT TTTAAAAAGA AAGTAGTAGA AGATGACTCA
    GAGAATTGCA CCTCTTCAGA AAGTTATGCG ACTGAAAGTA AAGACGACAA TTTTTTAGAT
    CCTATTTCCT TAAATTCTCG AGAATATTTC AACATTCATC TGTGGTTGAG GTGCCGCTTA
    GCCTTGGTGA CTGCATTTGT TGCCCAGATT CGTGGCATTG GAATCGTGAA AGAAAACGAT
    ATAACAGATT ACGTGAGCCT TATCAATGAA GTGTGTGTGG AGGCAAAAGC TGCAGGTGAC
    AGGGAGCTAC AGGCTGAATT CTTGATGCAA GCTGTAATTA TCGGCCTACA AGAAAAGCAT
    TTAAAGGCAG ACATCATAAA AAGCCTTCAG GAGGTAATAC AGTTGCTTGA AGGGCATGAC
    TTTATTTCTC CTCGATCTCA TTTAACCCTG GTGAGAAGCA TGCTTTTGCT GGATGATTTA
    ACAAAAGCTG AGAAATTCAA GGAAACTCCT TTTTCGAAAA TAGAAAAATT GTATTTGTTG
    ACTCAGTCTC ACAACATTCT TATAGAACAG ATGATAACTT TTGGAGAAAC AATTGAATTG
    CCTTCATCAA ACACTGACTA TGCAAGTCCA TTACTGCCTT TGAAAAATAT TTATCTTCCT
    CACGTCATGT TGTTGGCCAA AACAAAAATG AGAATTGGAC ATACAGTGGC CAAGCAAGTA
    TATTATACCA GTAAAAAGAA GGATTCTTCA AAGTGGTTAC CTGCCCTTCA TTTTTTTGAA
    ATAGCTTTGA AGCTCGCTAG GACATCAGGA ACAGAAGAAC ATGAGGTGGA AGCTGAAATC
    TTTTTTCAGA AAGGCAAAAT AGAATGTCAA ATATTGATGG AAGAGAAACC TCCAACTATG
    CAACTTGAGA GTCTGTTTGA AGCTATACAG CTAAGCTTGA GAAATGATCA AAATTCAGGG
    TTGATAAGAG ACTCCTACCT TGAAATAGCC TTATTATTTT TACACATGAG GAAGTTAAAG
    AGCAAACTTT CAGTGTCACC ATTGATGAGT ATTAAGGCTC TTCCCAGAAG GCATAGTTCT
    ATTAAAGAAC CAGTAGTAAA TAAACATGAA ATGTACAGTT TATTTGCCTG GATTGCAATA
    AGAGCTGCTG CACAGGTCAG TGAGGCTGTG CTGGCAATTA ATCTACTTAT TGGAAAGAAG
    AATGCTAGAA CTGATACAGT TAGCCAAGGG GCATTATCAA ATATTCCAGA ATTTGCTTCC
    ATGGATCTTT TGTCTTCATA CACAGATTAT TTGCTTGATA ACTACCAAGT TGTCTTCCAG
    ACTGGTTGTA CATTTTCTTA CGTTAGTGAA GAGATACATG AAGATGTAGA TTCTCAGAAA
    AAAAACCCAA TTAAAGTGGA TATTACATGG ATTCTTCTGC TGCGCTACTA TATTCACCTA
    CAAAGAATTA ATAATATGAG CAAATTGCTA GCATCTCTGA AGCCAGGATC TGGGATGTCT
    TTGCCAGATG ACACTCTTCT CACTTCTCTT TTTAACTCTG GATTGATTTT GCGCCAAAAA
    GAAATGCATT TTTTCCTTAA AAGATTCTTA CAGCTATATT CTTCTTCTTG TATTGATGAA
    TTTCCAAAAG AACTATTTCA AGTCATGGAA CATCCACCTG TTCTAGAAAA AGTCTTATAT
    GATTCAGCAA GCAAGACAGG AGTAACCAAT GCTTCTCTAT GCTCCGATTT ATCACTAAAG
    ACCGTTAGCA GCACATTGTA TGGAGACGTG GTGCCACCAG TTGTGGCAAC ACAGGCTTTA
    AACAAAGAAC TTTGTTTTCA GTGGTACATT CCTCCCCTGG AAAAACCTCC AAAGGAAACA
    GAACCTATGG TTTTATTGTT GTATGCATAT AATATGAAGC CCCTGAATAT TTCTGATGTT
    GCATCTGCTG CTTGCAACTG TGTGTACGTA GGCTCTTTCT GGGTTCCACT AAAAAGAGTT
    ATTTCAGTTC ATGAGAAACT ATCTAATCTT ACGCAAATTG CTGAACTATC ATTGCCAACA
    ACTCCTAAAG TTACTTCAAA TGAAAATTTA CATGAAGTAG AGGAAACAGA AGAGAAATCA
    ATTGATGCAG AAATGGAAAA CATGATTATT GTCTGTTGCA ATGAAATAGT ATCTTTGTTT
    TTGGGTGATG AAATGGCAAC TCCACTATCT GAGGTCCCAT TTGATGTCTC GCTGCCATCT
    ATATTCAGTC TGGAGAGACT TTTTGATCTT GCTAGTGGCT GTATCGTATC GGGAGGAAGC
    CTTTTCAACT GGGTGGTATC AATTATTCCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803563.2
    CDS929..10321
    Translation

    Target ORF information:

    RefSeq Version XM_010805261.3
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated protein 54 (CFAP54), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805261.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GATGTTCTTC AAACCAGCAA TCAAAGAAGT
    CTTCATGTGC AGGCGTTGCA TGAACTTGGA AATCTTCTCA TCTTCGCACA GAAGAAAAGG
    GCTGCTTTTA AGTGTTGGAG TCAAGCTCTG GATGAAATTT TCAGAAAAGA AGATGTGCTC
    CATACATGGA AGGAGATTGG CAGCTCACTC ACCAGTGCCA CTGATGGCTG GTCACCTCCA
    GGTTCCAAAG ACTACAGTGA GGAGCTCCTG TCCAAAGGCG GCATCTGGGG GTGTTTGCAG
    GGAGCGGTGA TATCAGCAAA GATAGCACAA TTTATTCAGA CATTGGATGT TGAAAAGAGA
    ACCAATTGTT GCCTTTTATC AGCATTACTC TTTCAGGGTT TGCTTAGAAC CACACTGCCG
    CATCCTAAAG CTGAACGTTG CTACGCTCAG TATGAAATCA CTCAGCTTCT CCCGGGCATC
    GAGCTCTTCT CAGACAGATA TCGGGCTGAC ATCTGCTCCG TCATTGCGAG TTTATATTAC
    GTCATCCGCG AGCTGCACTT TGCTAAGCAA AATCTAGTAA TTCTGCCTCT CCTTGCATTG
    TACCAATATT TTGTTTCTGT AATTTGCCAA GACCTAGTCA AAAATCTGGA AGCAAGAATC
    CTCAAGATAG AAGTCCTTAC AGATTTAGGA TTCTTTTCCG AAGCCTTTCA TGAGCTATCC
    CAGATATTTT ATGCGAAAAA CACACCTTCT TCAGTACCCA CCAGCTGTAA ACCTACTGGA
    AAAATGAAGC TATTTCAATC ATTTGACTCG GGAAAACCTC TTATTAGTAA AGAGAATCTG
    CAGGCACTTG ATGATTTAAT AAATAAAGGA ATGCCTGTAA TTCTGACTAC AGTCAGCCAG
    CAACATCTCT TAAATAAGTT TTTAGTTGTT AAAGCATACT TTTTCATAAA TGTGGCTGCA
    ACAATAAATT GTGTCCCAGA ACAGACAATG AAACCAGTCG GCCATGGAGT TATGAATGAG
    AAAAGCAAAC CAAATCTTCC AAACTTGAAA GAGATGTGTT TAAAGGATGA GGGAGGCTCA
    CTTTGTCATC TGACAAAATT GAAAGAGAAC TTTGCTCTTA GCACACTAAA GTCAATTTTA
    CTGACAGAAG CTGAAGACAG ACTCAACACC CTTCTGTCGG AGATGGACCA TCTAAGCCAT
    AAGGACCTCT CTCAGTGTTC TGCTGGCGAA CTGGAGATTG TAGTGGAGGT CAGACTGCAG
    CTGGCTGCCA TTGCCCTGCA GAGACACCGG GCAGCATACA GTGCTGCGAT AGTATTTTCC
    ACACTTAAAC TTCTCCAGGA TTCAAAACTT TTTAAAAAGA AAGTAGTAGA AGATGACTCA
    GAGAATTGCA CCTCTTCAGA AAGTTATGCG ACTGAAAGTA AAGACGACAA TTTTTTAGAT
    CCTATTTCCT TAAATTCTCG AGAATATTTC AACATTCATC TGTGGTTGAG GTGCCGCTTA
    GCCTTGGTGA CTGCATTTGT TGCCCAGATT CGTGGCATTG GAATCGTGAA AGAAAACGAT
    ATAACAGATT ACGTGAGCCT TATCAATGAA GTGTGTGTGG AGGCAAAAGC TGCAGGTGAC
    AGGGAGCTAC AGGCTGAATT CTTGATGCAA GCTGTAATTA TCGGCCTACA AGAAAAGCAT
    TTAAAGGCAG ACATCATAAA AAGCCTTCAG GAGGTAATAC AGTTGCTTGA AGGGCATGAC
    TTTATTTCTC CTCGATCTCA TTTAACCCTG GTGAGAAGCA TGCTTTTGCT GGATGATTTA
    ACAAAAGCTG AGAAATTCAA GGAAACTCCT TTTTCGAAAA TAGAAAAATT GTATTTGTTG
    ACTCAGTCTC ACAACATTCT TATAGAACAG ATGATAACTT TTGGAGAAAC AATTGAATTG
    CCTTCATCAA ACACTGACTA TGCAAGTCCA TTACTGCCTT TGAAAAATAT TTATCTTCCT
    CACGTCATGT TGTTGGCCAA AACAAAAATG AGAATTGGAC ATACAGTGGC CAAGCAAGTA
    TATTATACCA GTAAAAAGAA GGATTCTTCA AAGTGGTTAC CTGCCCTTCA TTTTTTTGAA
    ATAGCTTTGA AGCTCGCTAG GACATCAGGA ACAGAAGAAC ATGAGGTGGA AGCTGAAATC
    TTTTTTCAGA AAGGCAAAAT AGAATGTCAA ATATTGATGG AAGAGAAACC TCCAACTATG
    CAACTTGAGA GTCTGTTTGA AGCTATACAG CTAAGCTTGA GAAATGATCA AAATTCAGGG
    TTGATAAGAG ACTCCTACCT TGAAATAGCC TTATTATTTT TACACATGAG GAAGTTAAAG
    AGCAAACTTT CAGTGTCACC ATTGATGAGT ATTAAGGCTC TTCCCAGAAG GCATAGTTCT
    ATTAAAGAAC CAGTAGTAAA TAAACATGAA ATGTACAGTT TATTTGCCTG GATTGCAATA
    AGAGCTGCTG CACAGGTCAG TGAGGCTGTG CTGGCAATTA ATCTACTTAT TGGAAAGAAG
    AATGCTAGAA CTGATACAGT TAGCCAAGGG GCATTATCAA ATATTCCAGA ATTTGCTTCC
    ATGGATCTTT TGTCTTCATA CACAGATTAT TTGCTTGATA ACTACCAAGT TGTCTTCCAG
    ACTGGTTGTA CATTTTCTTA CGTTAGTGAA GAGATACATG AAGATGTAGA TTCTCAGAAA
    AAAAACCCAA TTAAAGTGGA TATTACATGG ATTCTTCTGC TGCGCTACTA TATTCACCTA
    CAAAGAATTA ATAATATGAG CAAATTGCTA GCATCTCTGA AGCCAGGATC TGGGATGTCT
    TTGCCAGATG ACACTCTTCT CACTTCTCTT TTTAACTCTG GATTGATTTT GCGCCAAAAA
    GAAATGCATT TTTTCCTTAA AAGATTCTTA CAGCTATATT CTTCTTCTTG TATTGATGAA
    TTTCCAAAAG AACTATTTCA AGTCATGGAA CATCCACCTG TTCTAGAAAA AGTCTTATAT
    GATTCAGCAA GCAAGACAGG AGTAACCAAT GCTTCTCTAT GCTCCGATTT ATCACTAAAG
    ACCGTTAGCA GCACATTGTA TGGAGACGTG GTGCCACCAG TTGTGGCAAC ACAGGCTTTA
    AACAAAGAAC TTTGTTTTCA GTGGTACATT CCTCCCCTGG AAAAACCTCC AAAGGAAACA
    GAACCTATGG TTTTATTGTT GTATGCATAT AATATGAAGC CCCTGAATAT TTCTGATGTT
    GCATCTGCTG CTTGCAACTG TGTGTACGTA GGCTCTTTCT GGGTTCCACT AAAAAGAGTT
    ATTTCAGTTC ATGAGAAACT ATCTAATCTT ACGCAAATTG CTGAACTATC ATTGCCAACA
    ACTCCTAAAG TTACTTCAAA TGAAAATTTA CATGAAGTAG AGGAAACAGA AGAGAAATCA
    ATTGATGCAG AAATGGAAAA CATGATTATT GTCTGTTGCA ATGAAATAGT ATCTTTGTTT
    TTGGGTGATG AAATGGCAAC TCCACTATCT GAGGTCCCAT TTGATGTCTC GCTGCCATCT
    ATATTCAGTC TGGAGAGACT TTTTGATCTT GCTAGTGGCT GTATCGTATC GGGAGGAAGC
    CTTTTCAACT GGGTGGTATC AATTATTCCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89906
    Clone ID Related Accession (Same CDS sequence) XM_010827774.2 , XM_010805261.3 , XM_010805261.2
    Accession Version XM_010805261.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805261.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GATGTTCTTC AAACCAGCAA TCAAAGAAGT
    CTTCATGTGC AGGCGTTGCA TGAACTTGGA AATCTTCTCA TCTTCGCACA GAAGAAAAGG
    GCTGCTTTTA AGTGTTGGAG TCAAGCTCTG GATGAAATTT TCAGAAAAGA AGATGTGCTC
    CATACATGGA AGGAGATTGG CAGCTCACTC ACCAGTGCCA CTGATGGCTG GTCACCTCCA
    GGTTCCAAAG ACTACAGTGA GGAGCTCCTG TCCAAAGGCG GCATCTGGGG GTGTTTGCAG
    GGAGCGGTGA TATCAGCAAA GATAGCACAA TTTATTCAGA CATTGGATGT TGAAAAGAGA
    ACCAATTGTT GCCTTTTATC AGCATTACTC TTTCAGGGTT TGCTTAGAAC CACACTGCCG
    CATCCTAAAG CTGAACGTTG CTACGCTCAG TATGAAATCA CTCAGCTTCT CCCGGGCATC
    GAGCTCTTCT CAGACAGATA TCGGGCTGAC ATCTGCTCCG TCATTGCGAG TTTATATTAC
    GTCATCCGCG AGCTGCACTT TGCTAAGCAA AATCTAGTAA TTCTGCCTCT CCTTGCATTG
    TACCAATATT TTGTTTCTGT AATTTGCCAA GACCTAGTCA AAAATCTGGA AGCAAGAATC
    CTCAAGATAG AAGTCCTTAC AGATTTAGGA TTCTTTTCCG AAGCCTTTCA TGAGCTATCC
    CAGATATTTT ATGCGAAAAA CACACCTTCT TCAGTACCCA CCAGCTGTAA ACCTACTGGA
    AAAATGAAGC TATTTCAATC ATTTGACTCG GGAAAACCTC TTATTAGTAA AGAGAATCTG
    CAGGCACTTG ATGATTTAAT AAATAAAGGA ATGCCTGTAA TTCTGACTAC AGTCAGCCAG
    CAACATCTCT TAAATAAGTT TTTAGTTGTT AAAGCATACT TTTTCATAAA TGTGGCTGCA
    ACAATAAATT GTGTCCCAGA ACAGACAATG AAACCAGTCG GCCATGGAGT TATGAATGAG
    AAAAGCAAAC CAAATCTTCC AAACTTGAAA GAGATGTGTT TAAAGGATGA GGGAGGCTCA
    CTTTGTCATC TGACAAAATT GAAAGAGAAC TTTGCTCTTA GCACACTAAA GTCAATTTTA
    CTGACAGAAG CTGAAGACAG ACTCAACACC CTTCTGTCGG AGATGGACCA TCTAAGCCAT
    AAGGACCTCT CTCAGTGTTC TGCTGGCGAA CTGGAGATTG TAGTGGAGGT CAGACTGCAG
    CTGGCTGCCA TTGCCCTGCA GAGACACCGG GCAGCATACA GTGCTGCGAT AGTATTTTCC
    ACACTTAAAC TTCTCCAGGA TTCAAAACTT TTTAAAAAGA AAGTAGTAGA AGATGACTCA
    GAGAATTGCA CCTCTTCAGA AAGTTATGCG ACTGAAAGTA AAGACGACAA TTTTTTAGAT
    CCTATTTCCT TAAATTCTCG AGAATATTTC AACATTCATC TGTGGTTGAG GTGCCGCTTA
    GCCTTGGTGA CTGCATTTGT TGCCCAGATT CGTGGCATTG GAATCGTGAA AGAAAACGAT
    ATAACAGATT ACGTGAGCCT TATCAATGAA GTGTGTGTGG AGGCAAAAGC TGCAGGTGAC
    AGGGAGCTAC AGGCTGAATT CTTGATGCAA GCTGTAATTA TCGGCCTACA AGAAAAGCAT
    TTAAAGGCAG ACATCATAAA AAGCCTTCAG GAGGTAATAC AGTTGCTTGA AGGGCATGAC
    TTTATTTCTC CTCGATCTCA TTTAACCCTG GTGAGAAGCA TGCTTTTGCT GGATGATTTA
    ACAAAAGCTG AGAAATTCAA GGAAACTCCT TTTTCGAAAA TAGAAAAATT GTATTTGTTG
    ACTCAGTCTC ACAACATTCT TATAGAACAG ATGATAACTT TTGGAGAAAC AATTGAATTG
    CCTTCATCAA ACACTGACTA TGCAAGTCCA TTACTGCCTT TGAAAAATAT TTATCTTCCT
    CACGTCATGT TGTTGGCCAA AACAAAAATG AGAATTGGAC ATACAGTGGC CAAGCAAGTA
    TATTATACCA GTAAAAAGAA GGATTCTTCA AAGTGGTTAC CTGCCCTTCA TTTTTTTGAA
    ATAGCTTTGA AGCTCGCTAG GACATCAGGA ACAGAAGAAC ATGAGGTGGA AGCTGAAATC
    TTTTTTCAGA AAGGCAAAAT AGAATGTCAA ATATTGATGG AAGAGAAACC TCCAACTATG
    CAACTTGAGA GTCTGTTTGA AGCTATACAG CTAAGCTTGA GAAATGATCA AAATTCAGGG
    TTGATAAGAG ACTCCTACCT TGAAATAGCC TTATTATTTT TACACATGAG GAAGTTAAAG
    AGCAAACTTT CAGTGTCACC ATTGATGAGT ATTAAGGCTC TTCCCAGAAG GCATAGTTCT
    ATTAAAGAAC CAGTAGTAAA TAAACATGAA ATGTACAGTT TATTTGCCTG GATTGCAATA
    AGAGCTGCTG CACAGGTCAG TGAGGCTGTG CTGGCAATTA ATCTACTTAT TGGAAAGAAG
    AATGCTAGAA CTGATACAGT TAGCCAAGGG GCATTATCAA ATATTCCAGA ATTTGCTTCC
    ATGGATCTTT TGTCTTCATA CACAGATTAT TTGCTTGATA ACTACCAAGT TGTCTTCCAG
    ACTGGTTGTA CATTTTCTTA CGTTAGTGAA GAGATACATG AAGATGTAGA TTCTCAGAAA
    AAAAACCCAA TTAAAGTGGA TATTACATGG ATTCTTCTGC TGCGCTACTA TATTCACCTA
    CAAAGAATTA ATAATATGAG CAAATTGCTA GCATCTCTGA AGCCAGGATC TGGGATGTCT
    TTGCCAGATG ACACTCTTCT CACTTCTCTT TTTAACTCTG GATTGATTTT GCGCCAAAAA
    GAAATGCATT TTTTCCTTAA AAGATTCTTA CAGCTATATT CTTCTTCTTG TATTGATGAA
    TTTCCAAAAG AACTATTTCA AGTCATGGAA CATCCACCTG TTCTAGAAAA AGTCTTATAT
    GATTCAGCAA GCAAGACAGG AGTAACCAAT GCTTCTCTAT GCTCCGATTT ATCACTAAAG
    ACCGTTAGCA GCACATTGTA TGGAGACGTG GTGCCACCAG TTGTGGCAAC ACAGGCTTTA
    AACAAAGAAC TTTGTTTTCA GTGGTACATT CCTCCCCTGG AAAAACCTCC AAAGGAAACA
    GAACCTATGG TTTTATTGTT GTATGCATAT AATATGAAGC CCCTGAATAT TTCTGATGTT
    GCATCTGCTG CTTGCAACTG TGTGTACGTA GGCTCTTTCT GGGTTCCACT AAAAAGAGTT
    ATTTCAGTTC ATGAGAAACT ATCTAATCTT ACGCAAATTG CTGAACTATC ATTGCCAACA
    ACTCCTAAAG TTACTTCAAA TGAAAATTTA CATGAAGTAG AGGAAACAGA AGAGAAATCA
    ATTGATGCAG AAATGGAAAA CATGATTATT GTCTGTTGCA ATGAAATAGT ATCTTTGTTT
    TTGGGTGATG AAATGGCAAC TCCACTATCT GAGGTCCCAT TTGATGTCTC GCTGCCATCT
    ATATTCAGTC TGGAGAGACT TTTTGATCTT GCTAGTGGCT GTATCGTATC GGGAGGAAGC
    CTTTTCAACT GGGTGGTATC AATTATTCCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803563.2
    CDS16..9408
    Translation

    Target ORF information:

    RefSeq Version XM_010805261.2
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805261.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGTTTTGCAG ACTCCAACTG TGAGCAAAAA TACATCTACC
    AAAGGTACAG AGAAGCTAAA AAAACCTGCA AGCCCTGATT GTTTTACAGA ATTAAGTGTG
    ATGAATAAAA TAAGGAAGAA TAAGCTATCT AAAGCGATTT ATTTGATGCA GAAAACTCTT
    CTAATATTTG AGAAAGATGC AACCTGTTCT TCTCTACAGA ACTACTTGAC GGAAGCATAT
    TCTTTGATTG AGAAGACCGA ATCAGAACAA AGTATACAAT ATTCATATCA GAAATATGTG
    GAAACTGCCA AAAGAAAGAA AAGCAGGGTC CCTCCTCCAC CCATCCTGCT ATCCCGAACT
    TACCGTTCTG TGACATTAAA ACCTGCCCCA TTTGTTTCTG ATGTTAAGGT CTGCTGGTAC
    TGCATTTTGG GTCGCAAAGC AGAAGGAAGC TATGGAAAAG TAAGACTAAA CGATAATCAT
    CTCCCAAACT CAGGAGAAGC GATACCAGCA GATGGCAAAA GCGTTTTTGA GGTAAAAGGT
    TTAGAAACCA ATGAAAAATA CGTCTTTGCA GTTGCGGCTT ACTGTTCTAA AGGAAAGCTG
    ATTGGTGATG CTATTGGCGA GACAACTAAA CCAATTCTGG TTTACCCACC TCTTTCTGCT
    GTTACTACTC GGATGTTATT GACGCAGGTC GCCTATCAGA CTGGTAACTA TGATCTGGCT
    AAGAAAGTTT TCTCACCAGT CTGGGATTAT TTTGTTGCTT CACCACCTCA GGGTGACCAA
    TCCATTATTT GTTTAAGCAA TATAATGACT ATTACACAGA GAAGATTACA TTCAGATATT
    TTGGCAGAGA GTTCTTCAAT TCTCTTATAT CTTTTTCTTA GAAATATTTT TGTAACAAGT
    GACATTAAAA TTAAAGAAGA AAATTTTTTC TGTGATAATA TTAAAGGCAA TGAGATTTTC
    CCTTCTCAAC AAATTGCTAG ACTAATTGAA TGTGAGAGAG TATTGGTGGC ATTGGAACTG
    AGCAACTACC TAAATGATTC CAGTTATGCC CTTCAGGCTG TGACTCAGTG TTACGGCCTC
    CTTGCTCCTA TCATTTATCA CAATATTGTT TTGGTACCTG TTGTACAGAT CTTGATAAAG
    TGTGTGGTAG TTTTGCAAGG ACTACCAAAT GTCATCCACT CAAAGAAACA TCTTGCAAGC
    TTTGAAAGTG TACAGCATAT GATAGCTTGC TGTATCTTCT ACATAACAAA GATCCTGCGT
    TCATGGAAGG AATATGACCT GGCAGTAATG ATTATAAATT ATGGAAAAAA GGTGTTGGAC
    ATCTCATCAG GTTGCAAATC TCTATTTGGT ACTACAGACC TAGAGGAAAT GCAAGAAGAG
    GGTTCTTCCA AGAAGTCTTG CAAGAATAAG AAGCCACAGC AGGTATTAGT ACCTGAAAAA
    ATAAATGAAC AGCTGGTTTT ATTGGAGACA CATCTACTCA AACTGACAAA GCAATATATT
    ACAACTGAAC TCTCAGGAGC AGAAGATCCC ATATTTCTTT ACCCTATAGT TTTAAACTGG
    TCGGTCAAAG GTGCTGTGAA AGAAGTTATG AAATTTAAGC AGAGACCTAG ATTCCTGGAA
    TTCTTTACAC AAGTTATGCA AAAATGTATG CATGATGAAA AATTTCAACC TATGGTGGAG
    ATAACAAATC CTGTCTATGA CTTCTTGAAA AGGAGGAATG AGTCCCTTCT TGGAATAAAA
    AAACTGAAAT ACAAGGATAC TTTGTCTAAA AAGGTCATCA AACCTGTGAA GAAGTACAGA
    GCTGCAGTAG TGGAGATTGG AAAAACTACA GAAATGAAGC AAAAAAAAAA AGCTAAAAGA
    AAGGAAACTC TAAGAGAATT TTTGAATAAA AATCCCTTTA TTTCTGAAAT GCCGGAGCAT
    GAAAGAAATA AGAGAGCTGA TGTTCGAAAA GTAGCACATC GATTCCTGGT GGAAAACTTG
    AATCCTTTAA TATTAAGTTA TGTTAAAAAG AAGAGGTTCC ATCAAATATT TCTTGAGGAG
    ATGCCCTGGA AAGCTCAGAT GAATCTGTAT CTGGCAAGCG CACACTTCAA CTTGCTTTTA
    ACAAAGCTAG GGGAACGTAC AAAGATAAAA TTTGGTTCTT CACCGATAAT GGTCAGTTTC
    CGATCATGTG ACCCTACTAT GTTCTCACTG TATAATTCAG GAACAGTATT ACCAACAGGA
    AGAATGACTC TAGAAAACTA TAAAGTGATG CTTGATTTCC TTCTTACAGC TAAAAAAAGA
    AAGGCTCACT TACCATCAGA TGCTGAAGAA TTTTTGGCAT TTCTTAATTC CAAAATGAGT
    GGTGAAAATG TTTCCAAGAC CCAAACGGTT TATGAATCAG ACTCTCAGTC GGGACTTAGC
    AGTAAAGAAA AGGATCGATC TGCAAATGTG GGTCTCTTGG ATCATTTTAT GAAAATCTTT
    TTATATTGCA GGAGAGCAAT GGTTCTTGCT CATCGTGGTG GCTATTGGAC TCTGCTTCAG
    AACTGCTGTC GGGCCCTTTG GAACTTTACT CAGGAGCTAC AAATACTGCA TAAACAAGCA
    GTGGATCTGT ATAAGACATT TCCTATTAGC CAGGATAGTT TCCTCTGTAT CTCTGTTTTA
    CCATTCTATT TGGGAGCAGA ATTACTTATT GACATGTTAA TAGAACTACA GAATACTGAT
    TCTATTAAGA CTATTGATGA AAAAGGAGAG TTCAGTGTCC CGAGTTGCTA TGGAAATATT
    AAAAATGATA ACGGTGGTTC CAGCCTTACC TTTGAGCATC CTCTGGATGA CGTCAATGTG
    GTCGATTTGA AATGGATCCA TGACTTTGTA TTAAAATCTC TGGAACTTGT CTATCAAGTA
    GAAAAATGGG AAACACTCGT ATCACTTGCC ATTCAGTTCA ATGCAGTTTC GCATGAAAGA
    TACACAGAGC AAGTGACTCC ACTGCTGGTT TACGCACAGC GCCAGCTTCT ACTGCAAATA
    TCCAAATGCA AGGGCCCAGA CATCAGCCAA CAGGCTTGTG TAAGATATGA GACTGAATAC
    AAACAGAAGA TAACCTGCCG AAATTTCATT GGAATACAGC TCAAGATTAA TCCTCCAACC
    AATAAAGGGA CAGCTATAGG ACAGCGAGCA GATGTCCTTA GAAAGCTTAT CTATTCAGAA
    TACAGCAGAG CCAAAGAGCT CCTCTGTGTG CCTGTGGACG TGACAGACAC TCTGAGATGT
    TTTAGAGAGA CCTTGGAAAA ATCCAAATAT CACAACAGAT CAATCCGGCA CAGCAGAAAG
    TTGCTTTCGT TATTTCTCGC ACAGACACAA GATGTTCTTC AAACCAGCAA TCAAAGAAGT
    CTTCATGTGC AGGCGTTGCA TGAACTTGGA AATCTTCTCA TCTTCGCACA GAAGAAAAGG
    GCTGCTTTTA AGTGTTGGAG TCAAGCTCTG GATGAAATTT TCAGAAAAGA AGATGTGCTC
    CATACATGGA AGGAGATTGG CAGCTCACTC ACCAGTGCCA CTGATGGCTG GTCACCTCCA
    GGTTCCAAAG ACTACAGTGA GGAGCTCCTG TCCAAAGGCG GCATCTGGGG GTGTTTGCAG
    GGAGCGGTGA TATCAGCAAA GATAGCACAA TTTATTCAGA CATTGGATGT TGAAAAGAGA
    ACCAATTGTT GCCTTTTATC AGCATTACTC TTTCAGGGTT TGCTTAGAAC CACACTGCCG
    CATCCTAAAG CTGAACGTTG CTACGCTCAG TATGAAATCA CTCAGCTTCT CCCGGGCATC
    GAGCTCTTCT CAGACAGATA TCGGGCTGAC ATCTGCTCCG TCATTGCGAG TTTATATTAC
    GTCATCCGCG AGCTGCACTT TGCTAAGCAA AATCTAGTAA TTCTGCCTCT CCTTGCATTG
    TACCAATATT TTGTTTCTGT AATTTGCCAA GACCTAGTCA AAAATCTGGA AGCAAGAATC
    CTCAAGATAG AAGTCCTTAC AGATTTAGGA TTCTTTTCCG AAGCCTTTCA TGAGCTATCC
    CAGATATTTT ATGCGAAAAA CACACCTTCT TCAGTACCCA CCAGCTGTAA ACCTACTGGA
    AAAATGAAGC TATTTCAATC ATTTGACTCG GGAAAACCTC TTATTAGTAA AGAGAATCTG
    CAGGCACTTG ATGATTTAAT AAATAAAGGA ATGCCTGTAA TTCTGACTAC AGTCAGCCAG
    CAACATCTCT TAAATAAGTT TTTAGTTGTT AAAGCATACT TTTTCATAAA TGTGGCTGCA
    ACAATAAATT GTGTCCCAGA ACAGACAATG AAACCAGTCG GCCATGGAGT TATGAATGAG
    AAAAGCAAAC CAAATCTTCC AAACTTGAAA GAGATGTGTT TAAAGGATGA GGGAGGCTCA
    CTTTGTCATC TGACAAAATT GAAAGAGAAC TTTGCTCTTA GCACACTAAA GTCAATTTTA
    CTGACAGAAG CTGAAGACAG ACTCAACACC CTTCTGTCGG AGATGGACCA TCTAAGCCAT
    AAGGACCTCT CTCAGTGTTC TGCTGGCGAA CTGGAGATTG TAGTGGAGGT CAGACTGCAG
    CTGGCTGCCA TTGCCCTGCA GAGACACCGG GCAGCATACA GTGCTGCGAT AGTATTTTCC
    ACACTTAAAC TTCTCCAGGA TTCAAAACTT TTTAAAAAGA AAGTAGTAGA AGATGACTCA
    GAGAATTGCA CCTCTTCAGA AAGTTATGCG ACTGAAAGTA AAGACGACAA TTTTTTAGAT
    CCTATTTCCT TAAATTCTCG AGAATATTTC AACATTCATC TGTGGTTGAG GTGCCGCTTA
    GCCTTGGTGA CTGCATTTGT TGCCCAGATT CGTGGCATTG GAATCGTGAA AGAAAACGAT
    ATAACAGATT ACGTGAGCCT TATCAATGAA GTGTGTGTGG AGGCAAAAGC TGCAGGTGAC
    AGGGAGCTAC AGGCTGAATT CTTGATGCAA GCTGTAATTA TCGGCCTACA AGAAAAGCAT
    TTAAAGGCAG ACATCATAAA AAGCCTTCAG GAGGTAATAC AGTTGCTTGA AGGGCATGAC
    TTTATTTCTC CTCGATCTCA TTTAACCCTG GTGAGAAGCA TGCTTTTGCT GGATGATTTA
    ACAAAAGCTG AGAAATTCAA GGAAACTCCT TTTTCGAAAA TAGAAAAATT GTATTTGTTG
    ACTCAGTCTC ACAACATTCT TATAGAACAG ATGATAACTT TTGGAGAAAC AATTGAATTG
    CCTTCATCAA ACACTGACTA TGCAAGTCCA TTACTGCCTT TGAAAAATAT TTATCTTCCT
    CACGTCATGT TGTTGGCCAA AACAAAAATG AGAATTGGAC ATACAGTGGC CAAGCAAGTA
    TATTATACCA GTAAAAAGAA GGATTCTTCA AAGTGGTTAC CTGCCCTTCA TTTTTTTGAA
    ATAGCTTTGA AGCTCGCTAG GACATCAGGA ACAGAAGAAC ATGAGGTGGA AGCTGAAATC
    TTTTTTCAGA AAGGCAAAAT AGAATGTCAA ATATTGATGG AAGAGAAACC TCCAACTATG
    CAACTTGAGA GTCTGTTTGA AGCTATACAG CTAAGCTTGA GAAATGATCA AAATTCAGGG
    TTGATAAGAG ACTCCTACCT TGAAATAGCC TTATTATTTT TACACATGAG GAAGTTAAAG
    AGCAAACTTT CAGTGTCACC ATTGATGAGT ATTAAGGCTC TTCCCAGAAG GCATAGTTCT
    ATTAAAGAAC CAGTAGTAAA TAAACATGAA ATGTACAGTT TATTTGCCTG GATTGCAATA
    AGAGCTGCTG CACAGGTCAG TGAGGCTGTG CTGGCAATTA ATCTACTTAT TGGAAAGAAG
    AATGCTAGAA CTGATACAGT TAGCCAAGGG GCATTATCAA ATATTCCAGA ATTTGCTTCC
    ATGGATCTTT TGTCTTCATA CACAGATTAT TTGCTTGATA ACTACCAAGT TGTCTTCCAG
    ACTGGTTGTA CATTTTCTTA CGTTAGTGAA GAGATACATG AAGATGTAGA TTCTCAGAAA
    AAAAACCCAA TTAAAGTGGA TATTACATGG ATTCTTCTGC TGCGCTACTA TATTCACCTA
    CAAAGAATTA ATAATATGAG CAAATTGCTA GCATCTCTGA AGCCAGGATC TGGGATGTCT
    TTGCCAGATG ACACTCTTCT CACTTCTCTT TTTAACTCTG GATTGATTTT GCGCCAAAAA
    GAAATGCATT TTTTCCTTAA AAGATTCTTA CAGCTATATT CTTCTTCTTG TATTGATGAA
    TTTCCAAAAG AACTATTTCA AGTCATGGAA CATCCACCTG TTCTAGAAAA AGTCTTATAT
    GATTCAGCAA GCAAGACAGG AGTAACCAAT GCTTCTCTAT GCTCCGATTT ATCACTAAAG
    ACCGTTAGCA GCACATTGTA TGGAGACGTG GTGCCACCAG TTGTGGCAAC ACAGGCTTTA
    AACAAAGAAC TTTGTTTTCA GTGGTACATT CCTCCCCTGG AAAAACCTCC AAAGGAAACA
    GAACCTATGG TTTTATTGTT GTATGCATAT AATATGAAGC CCCTGAATAT TTCTGATGTT
    GCATCTGCTG CTTGCAACTG TGTGTACGTA GGCTCTTTCT GGGTTCCACT AAAAAGAGTT
    ATTTCAGTTC ATGAGAAACT ATCTAATCTT ACGCAAATTG CTGAACTATC ATTGCCAACA
    ACTCCTAAAG TTACTTCAAA TGAAAATTTA CATGAAGTAG AGGAAACAGA AGAGAAATCA
    ATTGATGCAG AAATGGAAAA CATGATTATT GTCTGTTGCA ATGAAATAGT ATCTTTGTTT
    TTGGGTGATG AAATGGCAAC TCCACTATCT GAGGTCCCAT TTGATGTCTC GCTGCCATCT
    ATATTCAGTC TGGAGAGACT TTTTGATCTT GCTAGTGGCT GTATCGTATC GGGAGGAAGC
    CTTTTCAACT GGGTGGTATC AATTATTCCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89907
    Clone ID Related Accession (Same CDS sequence) XM_015463364.1 , XM_015471151.1
    Accession Version XM_015463364.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8406bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644803.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGATAACT
    ACCAAGTTGT CTTCCAGACT GGTTGTACAT TTTCTTACGT TAGTGAAGAG ATACATGAAG
    ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318850.1
    CDS30..8435
    Translation

    Target ORF information:

    RefSeq Version XM_015463364.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463364.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGATAACT
    ACCAAGTTGT CTTCCAGACT GGTTGTACAT TTTCTTACGT TAGTGAAGAG ATACATGAAG
    ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89907
    Clone ID Related Accession (Same CDS sequence) XM_015463364.1 , XM_015471151.1
    Accession Version XM_015471151.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8406bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cilia- and flagella-associated protein 54 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGATAACT
    ACCAAGTTGT CTTCCAGACT GGTTGTACAT TTTCTTACGT TAGTGAAGAG ATACATGAAG
    ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326637.1
    CDS30..8435
    Translation

    Target ORF information:

    RefSeq Version XM_015471151.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cilia and flagella associated 54 (CFAP54), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471151.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGCCTGGTT ACCACGAGAC CGTCGCCAGG CAACCGCGTG TACACACACA CCCCGGGACG 
    GGCCCGGGTC TCGTCAACAT GGCGGCGCAA GGCACCTGCT CGGTCTCTTC ACCAAACGTG
    TCTCCCACTT CAGTGTCGCC GCCGACTCCG CCCCCGCCCT CTACTGGGAT TTCGAAGTCG
    ACCCTGGTGG ACTCGAAGGC GAGCTCTCTG AGCCCACAGT CTCGCTCGGC CGGCCACGAG
    GACTTGTTGC CCCTCGCAGT ATTTTATGGG CCGCTGGACG CAAAAAACCC ACTCCTGGCC
    TCCTGTGAGA AGGAGATTCA GGAGTTGCTA GACTTCATGA AGAGAAAGAA GGCTTTAGCT
    ACAACGGAGG AACAGAAATA TGAGTTCCAC CGGCTTTGTG CCACCTCTCT GTTTAATATC
    TGGACCAAAT ACGCCCCCCG GCTGCCAGCT GCCTATTACA ACGAGAAGCT TCTGAAGGTT
    GGAGATAGCC TTTGTGAAAT GAAAGAATAT AAATTGGCCC TTTTACAGTG CTATGGCAGA
    TATCTTCAGG AGTTCAGCAT CAGCTTTGAT GAGAATAAAG CAGACGTGAA TCAATTCAAA
    ACTGTCTTTT TCCCAAAAGG CTTTGGAGAC AAAACTGCAG CACATACATT TCATGCTTTA
    AATGGCAAAA ATATTTGCAA CTACCAGCTG GTCTGTGACA GTGATGTGAA CCTGCAGAAT
    AAAGAATCTG TGACCCACTG TCTGCAAATC TTGTCCTCCT TCAGGCTCAT CATGCAGGTG
    GCTTTGCCAC AGGAGCACCT GTGCTGGATT ATCTTCAATG GTACCATTTA CATTTACACT
    ATTTGCAGAA AATTGATGAT CAGAGGTCAT TCTTCCAAGG CCTTGGAATA CCTCCTGTGG
    GCCAGTATAT GTATGGAGTC TTCAGTGCCT CTCCTGTCCA TCAGGTACTT GACGTGGAGA
    GCTACTCTTT ACACAGCTGT CTGCCAGTGC TACTATGACT GCCAAGCTGG TGTTCATGGA
    GAGGCATTTG CTCGGCGTGC TTTAGCTAAA ATTGATGAGT TACGGCAACT GGAATTAATG
    AGTTCCTCAC AACCACAGGA AGAATCCAGA GAATATTATA GAGAGGCCAC AATAAAGATG
    GCAGTCATGA TCTTCAAAAG AGGAGTATTT GAATCTAGAA GAAAAAACAA AAGTTTCTTT
    AGACCAAAGA TAAGAGTTAA CATAAAGGAA GCACAAACTA TGCCATGGCC GCGTACAGCC
    ACAGAAAAGC TGTTGGAGGA GTTGTTTGAT AGCACTGCCT CCCGGTTTCT GGCTGTCTTG
    GAAGCGCTCT CTGACTCAAA TCGGCGAATA CTACAGACTG GACCTGTTTT TACAGATGAA
    GTAGAAGTTC GTGATGTTGT CTCAGAACTG TTTATGGCAG GAAAAGAACT TTTAATAATG
    TCAAATATTG GTGCAGATGG CATACTTGAT TTTCCAAAAG CACATCTTTT GGAACTTATT
    ATAAAAAAGA AGAATCTTAT TTCTGTGAAT GCTGCCGTGA AATTTATAAA ATTAGCTTTC
    ATGTATGAGG AATGGAGTTT ATTTGAATCT GCGGCTGGGC AGCTCATTGA TTTTCTCCAG
    AGGCAGGATG ACCCAGCGTC AAAGAAAGCG GAAAAGAATT TAATTCTTCT TGTTGCGATA
    GAACCTTTGA TCAATGTAAA GAGAAACAAA GGTTTGATCT TTCCTTTTGA CAACTACAAA
    GAAGGTCAAG TCTATGTAAA AAAAATTGCT GTTCATGATA CTTATGTGAA GTCTTGTGGA
    TACTCAGAAG ACATTTTCCA TTTGGCGACA ACCTTGTATT CCTGTGTCTG TGACTCTCCA
    CAGAATGCTC AGCCGGATAA GGAAATCGTT GTGGACATGA TAATGTTCCT GTGGCAGAAA
    TGCAAATTAG GAATGCAGCG GGTCACTGTG TCCAAAAATG ACTACTCAAA GTTTACCCAG
    AAGATCAGTA CTAATAAATG GCTTTATCTT CTGTGGCAGA TAAATGAAAT CATTCACTGC
    TATAAGATGG AAGACATTGA TGTTGTGATG GTGGCAGAGA TCACGTTACG ATTAAGTGAA
    ATATTGGAAT CTTTAGGAAA CCCAAGAAGG AAATTTAAAA AATTTCTAGA TGTATCTTTA
    AAGAGAGGGA CTAGTGAAAT CTTTGGGGGT TCGAAAGGGA GCATAGATAT TCTTCCAATA
    ATAAAGAAAA AACCTGAAGA ACAGTTATTT ATGGCCTATG AACTTCTTGA CAAAGCCATT
    GATGGCATAA ATTTGAACTG CATGTTAACT GCTTTGCCAA ATGGATCATC AGTGATTGAC
    CATTGCTATG CCACGCACAA CCACTATATA GATGGAGACA TGTGCAAACC AGTCACACCG
    AATAATTTCA TAATGGATTT GCATCTTGAA CTAATTCAAG CCCAGCACCG AATAGCTGTT
    GTGCTTCTTG ACCAGTTGCA AGGTATTTTG CAGACTCCAA CTGTGAGCAA AAATACATCT
    ACCAAAGGTA CAGAGAAGCT AAAAAAACCT GCAAGCCCTG ATTGTTTTAC AGAATTAAGT
    GTGATGAATA AAATAAGGAA GAATAAGCTA TCTAAAGCGA TTTATTTGAT GCAGAAAACT
    CTTCTAATAT TTGAGAAAGA TGCAACCTGT TCTTCTCTAC AGAACTACTT GACGGAAGCA
    TATTCTTTGA TTGAGAAGAC CGAATCAGAA CAAAGTATAC AATATTCATA TCAGAAATAT
    GTGGAAACTG CCAAAAGAAA GAAAAGCAGG GTCCCTCCTC CACCCATCCT GCTATCCCGA
    ACTTACCGTT CTGTGACATT AAAACCTGCC CCATTTGTTT CTGATGTTAA GGTCTGCTGG
    TACTGCATTT TGGGTCGCAA AGCAGAAGGA AGCTATGGAA AAGTAAGACT AAACGATAAT
    CATCTCCCAA ACTCAGGAGA AGCGATACCA GCAGATGGCA AAAGCGTTTT TGAGGTAAAA
    GGTTTAGAAA CCAATGAAAA ATACGTCTTT GCAGTTGCGG CTTACTGTTC TAAAGGAAAG
    CTGATTGGTG ATGCTATTGG CGAGACAACT AAACCAATTC TGGTTTACCC ACCTCTTTCT
    GCTGTTACTA CTCGGATGTT ATTGACGCAG GTCGCCTATC AGACTGGTAA CTATGATCTG
    GCTAAGAAAG TTTTCTCACC AGTCTGGGAT TATTTTGTTG CTTCACCACC TCAGGGTGAC
    CAATCCATTA TTTGTTTAAG CAATATAATG ACTATTACAC AGAGAAGATT ACATTCAGAT
    ATTTTGGCAG AGAGTTCTTC AATTCTCTTA TATCTTTTTC TTAGAAATAT TTTTGTAACA
    AGTGACATTA AAATTAAAGA AGAAAATTTT TTCTGTGATA ATATTAAAGG CAATGAGATT
    TTCCCTTCTC AACAAATTGC TAGACTAATT GAATGTGAGA GAGTATTGGT GGCATTGGAA
    CTGAGCAACT ACCTAAATGA TTCCAGTTAT GCCCTTCAGG CTGTGACTCA GTGTTACGGC
    CTCCTTGCTC CTATCATTTA TCACAATATT GTTTTGGTAC CTGTTGTACA GATCTTGATA
    AAGTGTGTGG TAGTTTTGCA AGGACTACCA AATGTCATCC ACTCAAAGAA ACATCTTGCA
    AGCTTTGAAA GTGTACAGCA TATGATAGCT TGCTGTATCT TCTACATAAC AAAGATCCTG
    CGTTCATGGA AGGAATATGA CCTGGCAGTA ATGATTATAA ATTATGGAAA AAAGGTGTTG
    GACATCTCAT CAGGTTGCAA ATCTCTATTT GGTACTACAG ACCTAGAGGA AATGCAAGAA
    GAGGGTTCTT CCAAGAAGTC TTGCAAGAAT AAGAAGCCAC AGCAGGTATT AGTACCTGAA
    AAAATAAATG AACAGCTGGT TTTATTGGAG ACACATCTAC TCAAACTGAC AAAGCAATAT
    ATTACAACTG AACTCTCAGG AGCAGAAGAT CCCATATTTC TTTACCCTAT AGTTTTAAAC
    TGGTCGGTCA AAGGTGCTGT GAAAGAAGTT ATGAAATTTA AGCAGAGACC TAGATTCCTG
    GAATTCTTTA CACAAGTTAT GCAAAAATGT ATGCATGATG AAAAATTTCA ACCTATGGTG
    GAGATAACAA ATCCTGTCTA TGACTTCTTG AAAAGGAGGA ATGAGTCCCT TCTTGGAATA
    AAAAAACTGA AATACAAGGA TACTTTGTCT AAAAAGGTCA TCAAACCTGT GAAGAAGTAC
    AGAGCTGCAG TAGTGGAGAT TGGAAAAACT ACAGAAATGA AGCAAAAAAA AAAAGCTAAA
    AGAAAGGAAA CTCTAAGAGA ATTTTTGAAT AAAAATCCCT TTATTTCTGA AATGCCGGAG
    CATGAAAGAA ATAAGAGAGC TGATGTTCGA AAAGTAGCAC ATCGATTCCT GGTGGAAAAC
    TTGAATCCTT TAATATTAAG TTATGTTAAA AAGAAGAGGT TCCATCAAAT ATTTCTTGAG
    GAGATGCCCT GGAAAGCTCA GATGAATCTG TATCTGGCAA GCGCACACTT CAACTTGCTT
    TTAACAAAGC TAGGGGAACG TACAAAGATA AAATTTGGTT CTTCACCGAT AATGGTCAGT
    TTCCGATCAT GTGACCCTAC TATGTTCTCA CTGTATAATT CAGGAACAGT ATTACCAACA
    GGAAGAATGA CTCTAGAAAA CTATAAAGTG ATGCTTGATT TCCTTCTTAC AGCTAAAAAA
    AGAAAGGCTC ACTTACCATC AGATGCTGAA GAATTTTTGG CATTTCTTAA TTCCAAAATG
    AGTGGTGAAA ATGTTTCCAA GACCCAAACG GTTTATGAAT CAGACTCTCA GTCGGGACTT
    AGCAGTAAAG AAAAGGATCG ATCTGCAAAT GTGGGTCTCT TGGATCATTT TATGAAAATC
    TTTTTATATT GCAGGAGAGC AATGGTTCTT GCTCATCGTG GTGGCTATTG GACTCTGCTT
    CAGAACTGCT GTCGGGCCCT TTGGAACTTT ACTCAGGAGC TACAAATACT GCATAAACAA
    GCAGTGGATC TGTATAAGAC ATTTCCTATT AGCCAGGATA GTTTCCTCTG TATCTCTGTT
    TTACCATTCT ATTTGGGAGC AGAATTACTT ATTGACATGT TAATAGAACT ACAGAATACT
    GATTCTATTA AGACTATTGA TGAAAAAGGA GAGTTCAGTG TCCCGAGTTG CTATGGAAAT
    ATTAAAAATG ATAACGGTGG TTCCAGCCTT ACCTTTGAGC ATCCTCTGGA TGACGTCAAT
    GTGGTCGATT TGAAATGGAT CCATGACTTT GTATTAAAAT CTCTGGAACT TGTCTATCAA
    GTAGAAAAAT GGGAAACACT CGTATCACTT GCCATTCAGT TCAATGCAGT TTCGCATGAA
    AGATACACAG AGCAAGTGAC TCCACTGCTG GTTTACGCAC AGCGCCAGCT TCTACTGCAA
    ATATCCAAAT GCAAGGGCCC AGACATCAGC CAACAGGCTT GTGTAAGATA TGAGACTGAA
    TACAAACAGA AGATAACCTG CCGAAATTTC ATTGGAATAC AGCTCAAGAT TAATCCTCCA
    ACCAATAAAG GGACAGCTAT AGGACAGCGA GCAGATGTCC TTAGAAAGCT TATCTATTCA
    GAATACAGCA GAGCCAAAGA GCTCCTCTGT GTGCCTGTGG ACGTGACAGA CACTCTGAGA
    TGTTTTAGAG AGACCTTGGA AAAATCCAAA TATCACAACA GATCAATCCG GCACAGCAGA
    AAGTTGCTTT CGTTATTTCT CGCACAGACA CAAGGAGACA AAGGAGGAGT GAACAATTTA
    AAATTTGCTA CTGGGAAGGT GGAGTTTTCC ATGGGGACAG AAGAGATGCA CATGCCAACA
    CCCCCCGACC TTTCTCAGGA GCACTTCCGA GTTTTCAGCT CAGTGGAGAA GAGCAAGCTT
    CCCTCCTCAC AACTTGGACT CGTAATTTCT TCTTATTATG AAACTATTAA TGTTCTTCAA
    ACCAGCAATC AAAGAAGTCT TCATGTGCAG GCGTTGCATG AACTTGGAAA TCTTCTCATC
    TTCGCACAGA AGAAAAGGGC TGCTTTTAAG TGTTGGAGTC AAGCTCTGGA TGAAATTTTC
    AGAAAAGAAG ATGTGCTCCA TACATGGAAG GAGATTGGCA GCTCACTCAC CAGTGCCACT
    GATGGCTGGT CACCTCCAGG TTCCAAAGAC TACAGTGAGG AGCTCCTGTC CAAAGGCGGC
    ATCTGGGGGT GTTTGCAGGG AGCGGTGATA TCAGCAAAGA TAGCACAATT TATTCAGACA
    TTGGATGTTG AAAAGAGAAC CAATTGTTGC CTTTTATCAG CATTACTCTT TCAGGGTTTG
    CTTAGAACCA CACTGCCGCA TCCTAAAGCT GAACGTTGCT ACGCTCAGTA TGAAATCACT
    CAGCTTCTCC CGGGCATCGA GCTCTTCTCA GACAGATATC GGGCTGACAT CTGCTCCGTC
    ATTGCGAGTT TATATTACGT CATCCGCGAG CTGCACTTTG CTAAGCAAAA TCTAGTAATT
    CTGCCTCTCC TTGCATTGTA CCAATATTTT GTTTCTGTAA TTTGCCAAGA CCTAGTCAAA
    AATCTGGAAG CAAGAATCCT CAAGATAGAA GTCCTTACAG ATTTAGGATT CTTTTCCGAA
    GCCTTTCATG AGCTATCCCA GATATTTTAT GCGAAAAACA CACCTTCTTC AGTACCCACC
    AGCTGTAAAC CTACTGGAAA AATGAAGCTA TTTCAATCAT TTGACTCGGG AAAACCTCTT
    ATTAGTAAAG AGAATCTGCA GGCACTTGAT GATTTAATAA ATAAAGGAAT GCCTGTAATT
    CTGACTACAG TCAGCCAGCA ACATCTCTTA AATAAGTTTT TAGTTGTTAA AGCATACTTT
    TTCATAAATG TGGCTGCAAC AATAAATTGT GTCCCAGAAC AGACAATGAA ACCAGTCGGC
    CATGGAGTTA TGAATGAGAA AAGCAAACCA AATCTTCCAA ACTTGAAAGA GATGTGTTTA
    AAGGATGAGG GAGGCTCACT TTGTCATCTG ACAAAATTGA AAGAGAACTT TGCTCTTAGC
    ACACTAAAGT CAATTTTACT GACAGAAGCT GAAGACAGAC TCAACACCCT TCTGTCGGAG
    ATGGACCATC TAAGCCATAA GGACCTCTCT CAGTGTTCTG CTGGCGAACT GGAGATTGTA
    GTGGAGGTCA GACTGCAGCT GGCTGCCATT GCCCTGCAGA GACACCGGGC AGCATACAGT
    GCTGCGATAG TATTTTCCAC ACTTAAACTT CTCCAGGATT CAAAACTTTT TAAAAAGAAA
    GTAGTAGAAG ATGACTCAGA GAATTGCACC TCTTCAGAAA GTTATGCGAC TGAAAGTAAA
    GACGACAATT TTTTAGATCC TATTTCCTTA AATTCTCGAG AATATTTCAA CATTCATCTG
    TGGTTGAGGT GCCGCTTAGC CTTGGTGACT GCATTTGTTG CCCAGATTCG TGGCATTGGA
    ATCGTGAAAG AAAACGATAT AACAGATTAC GTGAGCCTTA TCAATGAAGT GTGTGTGGAG
    GCAAAAGCTG CAGGTGACAG GGAGCTACAG GCTGAATTCT TGATGCAAGC TGTAATTATC
    GGCCTACAAG AAAAGCATTT AAAGGCAGAC ATCATAAAAA GCCTTCAGGA GGTAATACAG
    TTGCTTGAAG GGCATGACTT TATTTCTCCT CGATCTCATT TAACCCTGGT GAGAAGCATG
    CTTTTGCTGG ATGATTTAAC AAAAGCTGAG AAATTCAAGG AAACTCCTTT TTCGAAAATA
    GAAAAATTGT ATTTGTTGAC TCAGTCTCAC AACATTCTTA TAGAACAGAT GATAACTTTT
    GGAGAAACAA TTGAATTGCC TTCATCAAAC ACTGACTATG CAAGTCCATT ACTGCCTTTG
    AAAAATATTT ATCTTCCTCA CGTCATGTTG TTGGCCAAAA CAAAAATGAG AATTGGACAT
    ACAGTGGCCA AGCAAGTATA TTATACCAGT AAAAAGAAGG ATTCTTCAAA GTGGTTACCT
    GCCCTTCATT TTTTTGAAAT AGCTTTGAAG CTCGCTAGGA CATCAGGAAC AGAAGAACAT
    GAGGTGGAAG CTGAAATCTT TTTTCAGAAA GGCAAAATAG AATGTCAAAT ATTGATGGAA
    GAGAAACCTC CAACTATGCA ACTTGAGAGT CTGTTTGAAG CTATACAGCT AAGCTTGAGA
    AATGATCAAA ATTCAGGGTT GATAAGAGAC TCCTACCTTG AAATAGCCTT ATTATTTTTA
    CACATGAGGA AGTTAAAGAG CAAACTTTCA GTGTCACCAT TGATGAGTAT TAAGATAACT
    ACCAAGTTGT CTTCCAGACT GGTTGTACAT TTTCTTACGT TAGTGAAGAG ATACATGAAG
    ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.