RALGAPB cDNA ORF clone, Bos taurus(cattle)

The following RALGAPB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RALGAPB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa222084 XM_025001236.1
Latest version!
Bos taurus Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa222085 XM_025001237.1
Latest version!
Bos taurus Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa58193 XM_010811700.2
Latest version!
Bos taurus Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa58193 XM_010824218.2
Latest version!
Bos taurus Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa222084
    Clone ID Related Accession (Same CDS sequence) XM_025001236.1
    Accession Version XM_025001236.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC GCTGCATTTG GTGATTCAGA ATGATCAGGG CCACACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTTCATCCT
    CTCGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGGAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACCGATTGGA TTATGGCTTT AGTGTTACCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GACCCGACTA
    TGCTTACAGG TCCTGAAAGC CATTCAAAAA CTGGCCCGTG AGTCATCCAT TATGGCCCGA
    GAAACCTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCGCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCATG TACGCGGTGC TTCCCAACAC CTCCTTACTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCACCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATCTGTGCGC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTTGCA
    CAGACGTGGT TTCGCTTTTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCAGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAACAG ATTGAGTATG CCTCAGAGCA CTGCTGTCAA TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCACACCTC AAAAGTTCAG CATCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCCCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCCGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA GCTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAACTG AGACGATCTT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCACATCA CTTTGGCACA GTCAAATCTG AGGTAGTCCT GGAAGGAAAG
    TTTAGTAATG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTTTGTC ACTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAG
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACTTCAAGA GTCACAGTCG CACAAATAGT
    GGTATTAGTT CAGCGAGTGG TGGAAGCACA GAACCCACAA CTCCTGACAG TGAAAGACCT
    GCTCAAGCTC TCCTGAGAGA TTATGCTCTT AATACAGATT CAGCTGCTGG GCTCCTGATT
    CGCAGCATTC ATCTCGTCAC TCAAAGACTC AATTCCCAGT GGCGGCAAGA CATGAGCATA
    TCACTGGCAG CTCTGGAGCT CCTCTCTGGA CTGGCAAAGG TGAAGGTGAT GGTTGACTCT
    GGAGACCGGA AGCGAGCCAT CAGTTCTGTG TGCGGCTACA TTGTGTACCA GTGTAGTCGG
    CCAGCTCCTC TTCACTCCCG GGACCTGCAC TCCATGATAG TGGCAGCTTT TCAGTGTCTC
    TGTGTCTGGC TGACAGAACA CCCCGACATG CTTGATGAAA AGGATTGCCT TAAGGAAGTG
    CTGGAAATTG TGGAACTGGG TATTTCAGGA AGCAAGTCCA AGAACAGTGA GCAAGAGGTC
    AAGTACAAAG GTGATAAGGA GCCAAACCCT GCCTCTATGA GAGTAAAGGA TGCTGCTGAA
    GCCACCCTAA CATGTATTAT GCAGTTGCTT GGCGCATTTC CTTCACCCAG TGGTCCTGCC
    TCTCCTTGTA GTCTGGTGAA CGAGACCACT TTGATCAAAT ACTCCAGACT GCCAACCATA
    AACAAACATA GTTTCCGGTA CTTTGTCTTG GATAACAGTG TCATCCTGGC AATGCTGGAG
    CAACCTCTTG GAAATGAACA GAATGATTTT TTCCCCTCTG TCACTGTGCT CGTCCGGGGA
    ATGTCTGGAA GACTTGCTTG GGCACAACAA CTTTGCCTTT TACCCAGAGG AGCAAAAGCA
    AATCAGAAGC TTTTTGTGCC TGAACCTCGT CCAGTTCCTA AAAATGATGT TGGACTTAAA
    TATACTGTGA AACATCGACC ATTTCCTGAA GAGGTGGATA AGATTCCTTT TGTGAAAGCA
    GATCTGAGCA TTCCAGATTT GCATGAAATC GTCACGGAAG AATTAGAAGA GAGACATGAA
    AAATTAAGGA ATGGCATGGC CCAGCAGATC GCTTATGAAA TACACCTTGA GCAACAAAGT
    GAGGAGGAAT TGCAGAAGAG AAGTTTTCCT GATCCAGTTA CAGATTGTAA GCCCCCACCC
    CCTGCCCAGG AATTCCAAAC AGCACGTCTT TTCCTTTCAC ACTTCGGATT TTTGTCTTTA
    GAAGCATTGA AGGAACCTGC AAACAGTCGT CTACCTCCTC ACCTTATTGC ACTTGACTCC
    ACGATACCTG GATTTTTTGA TGACATTGGG TATCTGGATC TCTTGCCATG TCGTCCCTTT
    GATACGGTTT TTATTTTCTA TATGAAGCCA GGCCAGAAAA CAAACCAGGA GATTTTAAAG
    AACGTGGAGT CTTCCAGAAA TGTTCAGCCA CATTTCCTAG AATTTTTGCT CTCCCTTGGC
    TGGTCAGTAG ATGTGGGCAA ACACCCTGGG TGGACTGGGC ATGTTTCCAC CAGTTGGTCT
    ATTAACAGTT GCGATGATGG TGAAGGACCT GAACAAGATG AAGTGATTTC CTCTGAAGAC
    GTTGGGGCTA GCATTTTCAA CGGACAGAAG AAGGTGCTGT ATTATGCTGA TGCCCTTACA
    GAAATAGCTT TTGTGGTTCC TTCTCCTGTG GAGTCCTTAA CTGATTCATT GGAAAGTAAC
    ATCTCAGACC AAGATAGTGA TTCAAATATG GATCTTATGC CAGGAATTCT GAAACAGCCA
    TCCCTCACAC TTGAGCTTTT CCCTAATCAC ACAGACAATC TTAATTCCTC ACAGAGGCTC
    AGTCCCAGTT CCAGAATGAA GAAGCTGCCT CAGGGTCGCC CTGTGCCTCC CCTCGGACCC
    GAGACAAGAG TTTCTGTAGT CTGGGTGGAA CGCTATGATG ATATAGAAAA CTTTCCCCTC
    TCAGACCTGA TGACAGAAAT CAGTACTGGT GTGGAAACTA CTGCAAACAG TAGCACTTCT
    CTGAGATCTA CTGCTCTTGA AAAAGAAGTT CCTGTCATCT TCATCCACCC TTTAAACACT
    GGATTATTCC GGATAAAAAT TCAAGGAGCC ACTGGAAAAT TTAACATGGT CATCCCTCTC
    GTGGACGGGA TGATTGTCAG TAGGCGAGCG CTCGGTTTTC TGGTGAGGCA GACTGTAATT
    AATATTTGTA GAAGAAAGAG ACTGGAGAGT GACTCCTACA GCCCACCACA CGTCCGCCGG
    AAACAGAAGA TCACTGACAT TGTCAACAAG TACCGGAACA AACAGTTGGA GCCAGAGTTT
    TACACTTCAC TTTTTCAGGA GGTTGGACTC AAAAACTGCA GTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857004.1
    CDS272..4759
    Translation

    Target ORF information:

    RefSeq Version XM_025001236.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001236.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC GCTGCATTTG GTGATTCAGA ATGATCAGGG CCACACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTTCATCCT
    CTCGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGGAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACCGATTGGA TTATGGCTTT AGTGTTACCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GACCCGACTA
    TGCTTACAGG TCCTGAAAGC CATTCAAAAA CTGGCCCGTG AGTCATCCAT TATGGCCCGA
    GAAACCTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCGCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCATG TACGCGGTGC TTCCCAACAC CTCCTTACTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCACCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATCTGTGCGC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTTGCA
    CAGACGTGGT TTCGCTTTTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCAGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAACAG ATTGAGTATG CCTCAGAGCA CTGCTGTCAA TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCACACCTC AAAAGTTCAG CATCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCCCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCCGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA GCTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAACTG AGACGATCTT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCACATCA CTTTGGCACA GTCAAATCTG AGGTAGTCCT GGAAGGAAAG
    TTTAGTAATG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTTTGTC ACTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAG
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACTTCAAGA GTCACAGTCG CACAAATAGT
    GGTATTAGTT CAGCGAGTGG TGGAAGCACA GAACCCACAA CTCCTGACAG TGAAAGACCT
    GCTCAAGCTC TCCTGAGAGA TTATGCTCTT AATACAGATT CAGCTGCTGG GCTCCTGATT
    CGCAGCATTC ATCTCGTCAC TCAAAGACTC AATTCCCAGT GGCGGCAAGA CATGAGCATA
    TCACTGGCAG CTCTGGAGCT CCTCTCTGGA CTGGCAAAGG TGAAGGTGAT GGTTGACTCT
    GGAGACCGGA AGCGAGCCAT CAGTTCTGTG TGCGGCTACA TTGTGTACCA GTGTAGTCGG
    CCAGCTCCTC TTCACTCCCG GGACCTGCAC TCCATGATAG TGGCAGCTTT TCAGTGTCTC
    TGTGTCTGGC TGACAGAACA CCCCGACATG CTTGATGAAA AGGATTGCCT TAAGGAAGTG
    CTGGAAATTG TGGAACTGGG TATTTCAGGA AGCAAGTCCA AGAACAGTGA GCAAGAGGTC
    AAGTACAAAG GTGATAAGGA GCCAAACCCT GCCTCTATGA GAGTAAAGGA TGCTGCTGAA
    GCCACCCTAA CATGTATTAT GCAGTTGCTT GGCGCATTTC CTTCACCCAG TGGTCCTGCC
    TCTCCTTGTA GTCTGGTGAA CGAGACCACT TTGATCAAAT ACTCCAGACT GCCAACCATA
    AACAAACATA GTTTCCGGTA CTTTGTCTTG GATAACAGTG TCATCCTGGC AATGCTGGAG
    CAACCTCTTG GAAATGAACA GAATGATTTT TTCCCCTCTG TCACTGTGCT CGTCCGGGGA
    ATGTCTGGAA GACTTGCTTG GGCACAACAA CTTTGCCTTT TACCCAGAGG AGCAAAAGCA
    AATCAGAAGC TTTTTGTGCC TGAACCTCGT CCAGTTCCTA AAAATGATGT TGGACTTAAA
    TATACTGTGA AACATCGACC ATTTCCTGAA GAGGTGGATA AGATTCCTTT TGTGAAAGCA
    GATCTGAGCA TTCCAGATTT GCATGAAATC GTCACGGAAG AATTAGAAGA GAGACATGAA
    AAATTAAGGA ATGGCATGGC CCAGCAGATC GCTTATGAAA TACACCTTGA GCAACAAAGT
    GAGGAGGAAT TGCAGAAGAG AAGTTTTCCT GATCCAGTTA CAGATTGTAA GCCCCCACCC
    CCTGCCCAGG AATTCCAAAC AGCACGTCTT TTCCTTTCAC ACTTCGGATT TTTGTCTTTA
    GAAGCATTGA AGGAACCTGC AAACAGTCGT CTACCTCCTC ACCTTATTGC ACTTGACTCC
    ACGATACCTG GATTTTTTGA TGACATTGGG TATCTGGATC TCTTGCCATG TCGTCCCTTT
    GATACGGTTT TTATTTTCTA TATGAAGCCA GGCCAGAAAA CAAACCAGGA GATTTTAAAG
    AACGTGGAGT CTTCCAGAAA TGTTCAGCCA CATTTCCTAG AATTTTTGCT CTCCCTTGGC
    TGGTCAGTAG ATGTGGGCAA ACACCCTGGG TGGACTGGGC ATGTTTCCAC CAGTTGGTCT
    ATTAACAGTT GCGATGATGG TGAAGGACCT GAACAAGATG AAGTGATTTC CTCTGAAGAC
    GTTGGGGCTA GCATTTTCAA CGGACAGAAG AAGGTGCTGT ATTATGCTGA TGCCCTTACA
    GAAATAGCTT TTGTGGTTCC TTCTCCTGTG GAGTCCTTAA CTGATTCATT GGAAAGTAAC
    ATCTCAGACC AAGATAGTGA TTCAAATATG GATCTTATGC CAGGAATTCT GAAACAGCCA
    TCCCTCACAC TTGAGCTTTT CCCTAATCAC ACAGACAATC TTAATTCCTC ACAGAGGCTC
    AGTCCCAGTT CCAGAATGAA GAAGCTGCCT CAGGGTCGCC CTGTGCCTCC CCTCGGACCC
    GAGACAAGAG TTTCTGTAGT CTGGGTGGAA CGCTATGATG ATATAGAAAA CTTTCCCCTC
    TCAGACCTGA TGACAGAAAT CAGTACTGGT GTGGAAACTA CTGCAAACAG TAGCACTTCT
    CTGAGATCTA CTGCTCTTGA AAAAGAAGTT CCTGTCATCT TCATCCACCC TTTAAACACT
    GGATTATTCC GGATAAAAAT TCAAGGAGCC ACTGGAAAAT TTAACATGGT CATCCCTCTC
    GTGGACGGGA TGATTGTCAG TAGGCGAGCG CTCGGTTTTC TGGTGAGGCA GACTGTAATT
    AATATTTGTA GAAGAAAGAG ACTGGAGAGT GACTCCTACA GCCCACCACA CGTCCGCCGG
    AAACAGAAGA TCACTGACAT TGTCAACAAG TACCGGAACA AACAGTTGGA GCCAGAGTTT
    TACACTTCAC TTTTTCAGGA GGTTGGACTC AAAAACTGCA GTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222085
    Clone ID Related Accession (Same CDS sequence) XM_025001237.1
    Accession Version XM_025001237.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4476bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ral GTPase-activating protein subunit beta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC GCTGCATTTG GTGATTCAGA ATGATCAGGG CCACACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTTCATCCT
    CTCGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGGAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACCGATTGGA TTATGGCTTT AGTGTTACCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GACCCGACTA
    TGCTTACAGG TCCTGAAAGC CATTCAAAAA CTGGCCCGTG AGTCATCCAT TATGGCCCGA
    GAAACCTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCGCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCATG TACGCGGTGC TTCCCAACAC CTCCTTACTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCACCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATCTGTGCGC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTTGCA
    CAGACGTGGT TTCGCTTTTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCAGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAACAG ATTGAGTATG CCTCAGAGCA CTGCTGTCAA TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCACACCTC AAAAGTTCAG CATCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCCCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCCGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA GCTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAACTG AGACGATCTT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCACATCA CTTTGGCACA GTCAAATCTG AGGTAGTCCT GGAAGGAAAG
    TTTAGTAATG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTTTGTC ACTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAG
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACTTCAAGA GTCACAGTCG CACAAATAGT
    GGTATTAGTT CAGCGAGTGG TGGAAGCACA GAACCCACAA CTCCTGACAG TGAAAGACCT
    GCTCAAGCTC TCCTGAGAGA TTATGATTCA GCTGCTGGGC TCCTGATTCG CAGCATTCAT
    CTCGTCACTC AAAGACTCAA TTCCCAGTGG CGGCAAGACA TGAGCATATC ACTGGCAGCT
    CTGGAGCTCC TCTCTGGACT GGCAAAGGTG AAGGTGATGG TTGACTCTGG AGACCGGAAG
    CGAGCCATCA GTTCTGTGTG CGGCTACATT GTGTACCAGT GTAGTCGGCC AGCTCCTCTT
    CACTCCCGGG ACCTGCACTC CATGATAGTG GCAGCTTTTC AGTGTCTCTG TGTCTGGCTG
    ACAGAACACC CCGACATGCT TGATGAAAAG GATTGCCTTA AGGAAGTGCT GGAAATTGTG
    GAACTGGGTA TTTCAGGAAG CAAGTCCAAG AACAGTGAGC AAGAGGTCAA GTACAAAGGT
    GATAAGGAGC CAAACCCTGC CTCTATGAGA GTAAAGGATG CTGCTGAAGC CACCCTAACA
    TGTATTATGC AGTTGCTTGG CGCATTTCCT TCACCCAGTG GTCCTGCCTC TCCTTGTAGT
    CTGGTGAACG AGACCACTTT GATCAAATAC TCCAGACTGC CAACCATAAA CAAACATAGT
    TTCCGGTACT TTGTCTTGGA TAACAGTGTC ATCCTGGCAA TGCTGGAGCA ACCTCTTGGA
    AATGAACAGA ATGATTTTTT CCCCTCTGTC ACTGTGCTCG TCCGGGGAAT GTCTGGAAGA
    CTTGCTTGGG CACAACAACT TTGCCTTTTA CCCAGAGGAG CAAAAGCAAA TCAGAAGCTT
    TTTGTGCCTG AACCTCGTCC AGTTCCTAAA AATGATGTTG GACTTAAATA TACTGTGAAA
    CATCGACCAT TTCCTGAAGA GGTGGATAAG ATTCCTTTTG TGAAAGCAGA TCTGAGCATT
    CCAGATTTGC ATGAAATCGT CACGGAAGAA TTAGAAGAGA GACATGAAAA ATTAAGGAAT
    GGCATGGCCC AGCAGATCGC TTATGAAATA CACCTTGAGC AACAAAGTGA GGAGGAATTG
    CAGAAGAGAA GTTTTCCTGA TCCAGTTACA GATTGTAAGC CCCCACCCCC TGCCCAGGAA
    TTCCAAACAG CACGTCTTTT CCTTTCACAC TTCGGATTTT TGTCTTTAGA AGCATTGAAG
    GAACCTGCAA ACAGTCGTCT ACCTCCTCAC CTTATTGCAC TTGACTCCAC GATACCTGGA
    TTTTTTGATG ACATTGGGTA TCTGGATCTC TTGCCATGTC GTCCCTTTGA TACGGTTTTT
    ATTTTCTATA TGAAGCCAGG CCAGAAAACA AACCAGGAGA TTTTAAAGAA CGTGGAGTCT
    TCCAGAAATG TTCAGCCACA TTTCCTAGAA TTTTTGCTCT CCCTTGGCTG GTCAGTAGAT
    GTGGGCAAAC ACCCTGGGTG GACTGGGCAT GTTTCCACCA GTTGGTCTAT TAACAGTTGC
    GATGATGGTG AAGGACCTGA ACAAGATGAA GTGATTTCCT CTGAAGACGT TGGGGCTAGC
    ATTTTCAACG GACAGAAGAA GGTGCTGTAT TATGCTGATG CCCTTACAGA AATAGCTTTT
    GTGGTTCCTT CTCCTGTGGA GTCCTTAACT GATTCATTGG AAAGTAACAT CTCAGACCAA
    GATAGTGATT CAAATATGGA TCTTATGCCA GGAATTCTGA AACAGCCATC CCTCACACTT
    GAGCTTTTCC CTAATCACAC AGACAATCTT AATTCCTCAC AGAGGCTCAG TCCCAGTTCC
    AGAATGAAGA AGCTGCCTCA GGGTCGCCCT GTGCCTCCCC TCGGACCCGA GACAAGAGTT
    TCTGTAGTCT GGGTGGAACG CTATGATGAT ATAGAAAACT TTCCCCTCTC AGACCTGATG
    ACAGAAATCA GTACTGGTGT GGAAACTACT GCAAACAGTA GCACTTCTCT GAGATCTACT
    GCTCTTGAAA AAGAAGTTCC TGTCATCTTC ATCCACCCTT TAAACACTGG ATTATTCCGG
    ATAAAAATTC AAGGAGCCAC TGGAAAATTT AACATGGTCA TCCCTCTCGT GGACGGGATG
    ATTGTCAGTA GGCGAGCGCT CGGTTTTCTG GTGAGGCAGA CTGTAATTAA TATTTGTAGA
    AGAAAGAGAC TGGAGAGTGA CTCCTACAGC CCACCACACG TCCGCCGGAA ACAGAAGATC
    ACTGACATTG TCAACAAGTA CCGGAACAAA CAGTTGGAGC CAGAGTTTTA CACTTCACTT
    TTTCAGGAGG TTGGACTCAA AAACTGCAGT TCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857005.1
    CDS272..4747
    Translation

    Target ORF information:

    RefSeq Version XM_025001237.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001237.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC GCTGCATTTG GTGATTCAGA ATGATCAGGG CCACACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTTCATCCT
    CTCGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGGAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACCGATTGGA TTATGGCTTT AGTGTTACCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GACCCGACTA
    TGCTTACAGG TCCTGAAAGC CATTCAAAAA CTGGCCCGTG AGTCATCCAT TATGGCCCGA
    GAAACCTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCGCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCATG TACGCGGTGC TTCCCAACAC CTCCTTACTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCACCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATCTGTGCGC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTTGCA
    CAGACGTGGT TTCGCTTTTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCAGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAACAG ATTGAGTATG CCTCAGAGCA CTGCTGTCAA TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCACACCTC AAAAGTTCAG CATCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCCCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCCGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA GCTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAACTG AGACGATCTT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCACATCA CTTTGGCACA GTCAAATCTG AGGTAGTCCT GGAAGGAAAG
    TTTAGTAATG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTTTGTC ACTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAG
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACTTCAAGA GTCACAGTCG CACAAATAGT
    GGTATTAGTT CAGCGAGTGG TGGAAGCACA GAACCCACAA CTCCTGACAG TGAAAGACCT
    GCTCAAGCTC TCCTGAGAGA TTATGATTCA GCTGCTGGGC TCCTGATTCG CAGCATTCAT
    CTCGTCACTC AAAGACTCAA TTCCCAGTGG CGGCAAGACA TGAGCATATC ACTGGCAGCT
    CTGGAGCTCC TCTCTGGACT GGCAAAGGTG AAGGTGATGG TTGACTCTGG AGACCGGAAG
    CGAGCCATCA GTTCTGTGTG CGGCTACATT GTGTACCAGT GTAGTCGGCC AGCTCCTCTT
    CACTCCCGGG ACCTGCACTC CATGATAGTG GCAGCTTTTC AGTGTCTCTG TGTCTGGCTG
    ACAGAACACC CCGACATGCT TGATGAAAAG GATTGCCTTA AGGAAGTGCT GGAAATTGTG
    GAACTGGGTA TTTCAGGAAG CAAGTCCAAG AACAGTGAGC AAGAGGTCAA GTACAAAGGT
    GATAAGGAGC CAAACCCTGC CTCTATGAGA GTAAAGGATG CTGCTGAAGC CACCCTAACA
    TGTATTATGC AGTTGCTTGG CGCATTTCCT TCACCCAGTG GTCCTGCCTC TCCTTGTAGT
    CTGGTGAACG AGACCACTTT GATCAAATAC TCCAGACTGC CAACCATAAA CAAACATAGT
    TTCCGGTACT TTGTCTTGGA TAACAGTGTC ATCCTGGCAA TGCTGGAGCA ACCTCTTGGA
    AATGAACAGA ATGATTTTTT CCCCTCTGTC ACTGTGCTCG TCCGGGGAAT GTCTGGAAGA
    CTTGCTTGGG CACAACAACT TTGCCTTTTA CCCAGAGGAG CAAAAGCAAA TCAGAAGCTT
    TTTGTGCCTG AACCTCGTCC AGTTCCTAAA AATGATGTTG GACTTAAATA TACTGTGAAA
    CATCGACCAT TTCCTGAAGA GGTGGATAAG ATTCCTTTTG TGAAAGCAGA TCTGAGCATT
    CCAGATTTGC ATGAAATCGT CACGGAAGAA TTAGAAGAGA GACATGAAAA ATTAAGGAAT
    GGCATGGCCC AGCAGATCGC TTATGAAATA CACCTTGAGC AACAAAGTGA GGAGGAATTG
    CAGAAGAGAA GTTTTCCTGA TCCAGTTACA GATTGTAAGC CCCCACCCCC TGCCCAGGAA
    TTCCAAACAG CACGTCTTTT CCTTTCACAC TTCGGATTTT TGTCTTTAGA AGCATTGAAG
    GAACCTGCAA ACAGTCGTCT ACCTCCTCAC CTTATTGCAC TTGACTCCAC GATACCTGGA
    TTTTTTGATG ACATTGGGTA TCTGGATCTC TTGCCATGTC GTCCCTTTGA TACGGTTTTT
    ATTTTCTATA TGAAGCCAGG CCAGAAAACA AACCAGGAGA TTTTAAAGAA CGTGGAGTCT
    TCCAGAAATG TTCAGCCACA TTTCCTAGAA TTTTTGCTCT CCCTTGGCTG GTCAGTAGAT
    GTGGGCAAAC ACCCTGGGTG GACTGGGCAT GTTTCCACCA GTTGGTCTAT TAACAGTTGC
    GATGATGGTG AAGGACCTGA ACAAGATGAA GTGATTTCCT CTGAAGACGT TGGGGCTAGC
    ATTTTCAACG GACAGAAGAA GGTGCTGTAT TATGCTGATG CCCTTACAGA AATAGCTTTT
    GTGGTTCCTT CTCCTGTGGA GTCCTTAACT GATTCATTGG AAAGTAACAT CTCAGACCAA
    GATAGTGATT CAAATATGGA TCTTATGCCA GGAATTCTGA AACAGCCATC CCTCACACTT
    GAGCTTTTCC CTAATCACAC AGACAATCTT AATTCCTCAC AGAGGCTCAG TCCCAGTTCC
    AGAATGAAGA AGCTGCCTCA GGGTCGCCCT GTGCCTCCCC TCGGACCCGA GACAAGAGTT
    TCTGTAGTCT GGGTGGAACG CTATGATGAT ATAGAAAACT TTCCCCTCTC AGACCTGATG
    ACAGAAATCA GTACTGGTGT GGAAACTACT GCAAACAGTA GCACTTCTCT GAGATCTACT
    GCTCTTGAAA AAGAAGTTCC TGTCATCTTC ATCCACCCTT TAAACACTGG ATTATTCCGG
    ATAAAAATTC AAGGAGCCAC TGGAAAATTT AACATGGTCA TCCCTCTCGT GGACGGGATG
    ATTGTCAGTA GGCGAGCGCT CGGTTTTCTG GTGAGGCAGA CTGTAATTAA TATTTGTAGA
    AGAAAGAGAC TGGAGAGTGA CTCCTACAGC CCACCACACG TCCGCCGGAA ACAGAAGATC
    ACTGACATTG TCAACAAGTA CCGGAACAAA CAGTTGGAGC CAGAGTTTTA CACTTCACTT
    TTTCAGGAGG TTGGACTCAA AAACTGCAGT TCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58193
    Clone ID Related Accession (Same CDS sequence) XM_010811700.2 , XM_010824218.2
    Accession Version XM_010811700.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4476bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: ral GTPase-activating protein subunit beta
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811700.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC GCTGCATTTG GTGATTCAGA ATGATCAGGG CCACACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTTCATCCT
    CTCGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGGAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACCGATTGGA TTATGGCTTT AGTGTTACCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GACCCGACTA
    TGCTTACAGG TCCTGAAAGC CATTCAAAAA CTGGCCCGTG AGTCATCCAT TATGGCCCGA
    GAAACCTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCGCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCATG TACGCGGTGC TTCCCAACAC CTCCTTACTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCACCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATCTGTGCGC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTTGCA
    CAGACGTGGT TTCGCTTTTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCAGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAACAG ATTGAGTATG CCTCAGAGCA CTGCTGTCAA TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCACACCTC AAAAGTTCAG CATCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCCCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCCGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA GCTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAACTG AGACGATCTT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCACATCA CTTTGGCACA GTCAAATCTG AGGTAAGGTT TTATTAAAGT
    TTTTCTAATG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTTTGTC ACTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAG
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACTTCAAGA GTCACAGTCG CACAAATAGT
    GGTATTAGTT CAGCGAGTGG TGGAAGCACA GAACCCACAA CTCCTGACAG TGAAAGACCT
    GCTCAAGCTC TCCTGAGAGA TTATGATTCA GCTGCTGGGC TCCTGATTCG CAGCATTCAT
    CTCGTCACTC AAAGACTCAA TTCCCAGTGG CGGCAAGACA TGAGCATATC ACTGGCAGCT
    CTGGAGCTCC TCTCTGGACT GGCAAAGGTG AAGGTGATGG TTGACTCTGG AGACCGGAAG
    CGAGCCATCA GTTCTGTGTG CGGCTACATT GTGTACCAGT GTAGTCGGCC AGCTCCTCTT
    CACTCCCGGG ACCTGCACTC CATGATAGTG GCAGCTTTTC AGTGTCTCTG TGTCTGGCTG
    ACAGAACACC CCGACATGCT TGATGAAAAG GATTGCCTTA AGGAAGTGCT GGAAATTGTG
    GAACTGGGTA TTTCAGGAAG CAAGTCCAAG AACAGTGAGC AAGAGGTCAA GTACAAAGGT
    GATAAGGAGC CAAACCCTGC CTCTATGAGA GTAAAGGATG CTGCTGAAGC CACCCTAACA
    TGTATTATGC AGTTGCTTGG CGCATTTCCT TCACCCAGTG GTCCTGCCTC TCCTTGTAGT
    CTGGTGAACG AGACCACTTT GATCAAATAC TCCAGACTGC CAACCATAAA CAAACATAGT
    TTCCGGTACT TTGTCTTGGA TAACAGTGTC ATCCTGGCAA TGCTGGAGCA ACCTCTTGGA
    AATGAACAGA ATGATTTTTT CCCCTCTGTC ACTGTGCTCG TCCGGGGAAT GTCTGGAAGA
    CTTGCTTGGG CACAACAACT TTGCCTTTTA CCCAGAGGAG CAAAAGCAAA TCAGAAGCTT
    TTTGTGCCTG AACCTCGTCC AGTTCCTAAA AATGATGTTG GACTTAAATA TACTGTGAAA
    CATCGACCAT TTCCTGAAGA GGTGGATAAG ATTCCTTTTG TGAAAGCAGA TCTGAGCATT
    CCAGATTTGC ATGAAATCGT CACGGAAGAA TTAGAAGAGA GACATGAAAA ATTAAGGAAT
    GGCATGGCCC AGCAGATCGC TTATGAAATA CACCTTGAGC AACAAAGTGA GGAGGAATTG
    CAGAAGAGAA GTTTTCCTGA TCCAGTTACA GATTGTAAGC CCCCACCCCC TGCCCAGGAA
    TTCCAAACAG CACGTCTTTT CCTTTCACAC TTCGGATTTT TGTCTTTAGA AGCATTGAAG
    GAACCTGCAA ACAGTCGTCT ACCTCCTCAC CTTATTGCAC TTGACTCCAC GATACCTGGA
    TTTTTTGATG ACATTGGGTA TCTGGATCTC TTGCCATGTC GTCCCTTTGA TACGGTTTTT
    ATTTTCTATA TGAAGCCAGG CCAGAAAACA AACCAGGAGA TTTTAAAGAA CGTGGAGTCT
    TCCAGAAATG TTCAGCCACA TTTCCTAGAA TTTTTGCTCT CCCTTGGCTG GTCAGTAGAT
    GTGGGCAAAC ACCCTGGGTG GACTGGGCAT GTTTCCACCA GTTGGTCTAT TAACAGTTGC
    GATGATGGTG AAGGACCTGA ACAAGATGAA GTGATTTCCT CTGAAGACGT TGGGGCTAGC
    ATTTTCAACG GACAGAAGAA GGTGCTGTAT TATGCTGATG CCCTTACAGA AATAGCTTTT
    GTGGTTCCTT CTCCTGTGGA GTCCTTAACT GATTCATTGG AAAGTAACAT CTCAGACCAA
    GATAGTGATT CAAATATGGA TCTTATGCCA GGAATTCTGA AACAGCCATC CCTCACACTT
    GAGCTTTTCC CTAATCACAC AGACAATCTT AATTCCTCAC AGAGGCTCAG TCCCAGTTCC
    AGAATGAAGA AGCTGCCTCA GGGTCGCCCT GTGCCTCCCC TCGGACCCGA GACAAGAGTT
    TCTGTAGTCT GGGTGGAACG CTATGATGAT ATAGAAAACT TTCCCCTCTC AGACCTGATG
    ACAGAAATCA GTACTGGTGT GGAAACTACT GCAAACAGTA GCACTTCTCT GAGATCTACT
    GCTCTTGAAA AAGAAGTTCC TGTCATCTTC ATCCACCCTT TAAACACTGG ATTATTCCGG
    ATAAAAATTC AAGGAGCCAC TGGAAAATTT AACATGGTCA TCCCTCTCGT GGACGGGATG
    ATTGTCAGTA GGCGAGCGCT CGGTTTTCTG GTGAGGCAGA CTGTAATTAA TATTTGTAGA
    AGAAAGAGAC TGGAGAGTGA CTCCTACAGC CCACCACACG TCCGCCGGAA ACAGAAGATC
    ACTGACATTG TCAACAAGTA CCGGAACAAA CAGTTGGAGC CAGAGTTTTA CACTTCACTT
    TTTCAGGAGG TTGGACTCAA AAACTGCAGT TCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810002.1
    CDS275..4750
    Translation

    Target ORF information:

    RefSeq Version XM_010811700.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010811700.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC GCTGCATTTG GTGATTCAGA ATGATCAGGG CCACACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTTCATCCT
    CTCGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGGAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACCGATTGGA TTATGGCTTT AGTGTTACCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GACCCGACTA
    TGCTTACAGG TCCTGAAAGC CATTCAAAAA CTGGCCCGTG AGTCATCCAT TATGGCCCGA
    GAAACCTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCGCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCATG TACGCGGTGC TTCCCAACAC CTCCTTACTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCACCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATCTGTGCGC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTTGCA
    CAGACGTGGT TTCGCTTTTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCAGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAACAG ATTGAGTATG CCTCAGAGCA CTGCTGTCAA TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCACACCTC AAAAGTTCAG CATCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCCCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCCGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA GCTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAACTG AGACGATCTT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCACATCA CTTTGGCACA GTCAAATCTG AGGTAAGGTT TTATTAAAGT
    TTTTCTAATG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTTTGTC ACTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAG
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACTTCAAGA GTCACAGTCG CACAAATAGT
    GGTATTAGTT CAGCGAGTGG TGGAAGCACA GAACCCACAA CTCCTGACAG TGAAAGACCT
    GCTCAAGCTC TCCTGAGAGA TTATGATTCA GCTGCTGGGC TCCTGATTCG CAGCATTCAT
    CTCGTCACTC AAAGACTCAA TTCCCAGTGG CGGCAAGACA TGAGCATATC ACTGGCAGCT
    CTGGAGCTCC TCTCTGGACT GGCAAAGGTG AAGGTGATGG TTGACTCTGG AGACCGGAAG
    CGAGCCATCA GTTCTGTGTG CGGCTACATT GTGTACCAGT GTAGTCGGCC AGCTCCTCTT
    CACTCCCGGG ACCTGCACTC CATGATAGTG GCAGCTTTTC AGTGTCTCTG TGTCTGGCTG
    ACAGAACACC CCGACATGCT TGATGAAAAG GATTGCCTTA AGGAAGTGCT GGAAATTGTG
    GAACTGGGTA TTTCAGGAAG CAAGTCCAAG AACAGTGAGC AAGAGGTCAA GTACAAAGGT
    GATAAGGAGC CAAACCCTGC CTCTATGAGA GTAAAGGATG CTGCTGAAGC CACCCTAACA
    TGTATTATGC AGTTGCTTGG CGCATTTCCT TCACCCAGTG GTCCTGCCTC TCCTTGTAGT
    CTGGTGAACG AGACCACTTT GATCAAATAC TCCAGACTGC CAACCATAAA CAAACATAGT
    TTCCGGTACT TTGTCTTGGA TAACAGTGTC ATCCTGGCAA TGCTGGAGCA ACCTCTTGGA
    AATGAACAGA ATGATTTTTT CCCCTCTGTC ACTGTGCTCG TCCGGGGAAT GTCTGGAAGA
    CTTGCTTGGG CACAACAACT TTGCCTTTTA CCCAGAGGAG CAAAAGCAAA TCAGAAGCTT
    TTTGTGCCTG AACCTCGTCC AGTTCCTAAA AATGATGTTG GACTTAAATA TACTGTGAAA
    CATCGACCAT TTCCTGAAGA GGTGGATAAG ATTCCTTTTG TGAAAGCAGA TCTGAGCATT
    CCAGATTTGC ATGAAATCGT CACGGAAGAA TTAGAAGAGA GACATGAAAA ATTAAGGAAT
    GGCATGGCCC AGCAGATCGC TTATGAAATA CACCTTGAGC AACAAAGTGA GGAGGAATTG
    CAGAAGAGAA GTTTTCCTGA TCCAGTTACA GATTGTAAGC CCCCACCCCC TGCCCAGGAA
    TTCCAAACAG CACGTCTTTT CCTTTCACAC TTCGGATTTT TGTCTTTAGA AGCATTGAAG
    GAACCTGCAA ACAGTCGTCT ACCTCCTCAC CTTATTGCAC TTGACTCCAC GATACCTGGA
    TTTTTTGATG ACATTGGGTA TCTGGATCTC TTGCCATGTC GTCCCTTTGA TACGGTTTTT
    ATTTTCTATA TGAAGCCAGG CCAGAAAACA AACCAGGAGA TTTTAAAGAA CGTGGAGTCT
    TCCAGAAATG TTCAGCCACA TTTCCTAGAA TTTTTGCTCT CCCTTGGCTG GTCAGTAGAT
    GTGGGCAAAC ACCCTGGGTG GACTGGGCAT GTTTCCACCA GTTGGTCTAT TAACAGTTGC
    GATGATGGTG AAGGACCTGA ACAAGATGAA GTGATTTCCT CTGAAGACGT TGGGGCTAGC
    ATTTTCAACG GACAGAAGAA GGTGCTGTAT TATGCTGATG CCCTTACAGA AATAGCTTTT
    GTGGTTCCTT CTCCTGTGGA GTCCTTAACT GATTCATTGG AAAGTAACAT CTCAGACCAA
    GATAGTGATT CAAATATGGA TCTTATGCCA GGAATTCTGA AACAGCCATC CCTCACACTT
    GAGCTTTTCC CTAATCACAC AGACAATCTT AATTCCTCAC AGAGGCTCAG TCCCAGTTCC
    AGAATGAAGA AGCTGCCTCA GGGTCGCCCT GTGCCTCCCC TCGGACCCGA GACAAGAGTT
    TCTGTAGTCT GGGTGGAACG CTATGATGAT ATAGAAAACT TTCCCCTCTC AGACCTGATG
    ACAGAAATCA GTACTGGTGT GGAAACTACT GCAAACAGTA GCACTTCTCT GAGATCTACT
    GCTCTTGAAA AAGAAGTTCC TGTCATCTTC ATCCACCCTT TAAACACTGG ATTATTCCGG
    ATAAAAATTC AAGGAGCCAC TGGAAAATTT AACATGGTCA TCCCTCTCGT GGACGGGATG
    ATTGTCAGTA GGCGAGCGCT CGGTTTTCTG GTGAGGCAGA CTGTAATTAA TATTTGTAGA
    AGAAAGAGAC TGGAGAGTGA CTCCTACAGC CCACCACACG TCCGCCGGAA ACAGAAGATC
    ACTGACATTG TCAACAAGTA CCGGAACAAA CAGTTGGAGC CAGAGTTTTA CACTTCACTT
    TTTCAGGAGG TTGGACTCAA AAACTGCAGT TCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58193
    Clone ID Related Accession (Same CDS sequence) XM_010811700.2 , XM_010824218.2
    Accession Version XM_010824218.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4476bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: ral GTPase-activating protein subunit beta
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646145.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824218.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC GCTGCATTTG GTGATTCAGA ATGATCAGGG CCACACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTTCATCCT
    CTCGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGGAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACCGATTGGA TTATGGCTTT AGTGTTACCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GACCCGACTA
    TGCTTACAGG TCCTGAAAGC CATTCAAAAA CTGGCCCGTG AGTCATCCAT TATGGCCCGA
    GAAACCTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCGCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCATG TACGCGGTGC TTCCCAACAC CTCCTTACTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCACCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATCTGTGCGC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTTGCA
    CAGACGTGGT TTCGCTTTTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCAGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAACAG ATTGAGTATG CCTCAGAGCA CTGCTGTCAA TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCACACCTC AAAAGTTCAG CATCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCCCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCCGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA GCTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAACTG AGACGATCTT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCACATCA CTTTGGCACA GTCAAATCTG AGGTAAGGTT TTATTAAAGT
    TTTTCTAATG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTTTGTC ACTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAG
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACTTCAAGA GTCACAGTCG CACAAATAGT
    GGTATTAGTT CAGCGAGTGG TGGAAGCACA GAACCCACAA CTCCTGACAG TGAAAGACCT
    GCTCAAGCTC TCCTGAGAGA TTATGATTCA GCTGCTGGGC TCCTGATTCG CAGCATTCAT
    CTCGTCACTC AAAGACTCAA TTCCCAGTGG CGGCAAGACA TGAGCATATC ACTGGCAGCT
    CTGGAGCTCC TCTCTGGACT GGCAAAGGTG AAGGTGATGG TTGACTCTGG AGACCGGAAG
    CGAGCCATCA GTTCTGTGTG CGGCTACATT GTGTACCAGT GTAGTCGGCC AGCTCCTCTT
    CACTCCCGGG ACCTGCACTC CATGATAGTG GCAGCTTTTC AGTGTCTCTG TGTCTGGCTG
    ACAGAACACC CCGACATGCT TGATGAAAAG GATTGCCTTA AGGAAGTGCT GGAAATTGTG
    GAACTGGGTA TTTCAGGAAG CAAGTCCAAG AACAGTGAGC AAGAGGTCAA GTACAAAGGT
    GATAAGGAGC CAAACCCTGC CTCTATGAGA GTAAAGGATG CTGCTGAAGC CACCCTAACA
    TGTATTATGC AGTTGCTTGG CGCATTTCCT TCACCCAGTG GTCCTGCCTC TCCTTGTAGT
    CTGGTGAACG AGACCACTTT GATCAAATAC TCCAGACTGC CAACCATAAA CAAACATAGT
    TTCCGGTACT TTGTCTTGGA TAACAGTGTC ATCCTGGCAA TGCTGGAGCA ACCTCTTGGA
    AATGAACAGA ATGATTTTTT CCCCTCTGTC ACTGTGCTCG TCCGGGGAAT GTCTGGAAGA
    CTTGCTTGGG CACAACAACT TTGCCTTTTA CCCAGAGGAG CAAAAGCAAA TCAGAAGCTT
    TTTGTGCCTG AACCTCGTCC AGTTCCTAAA AATGATGTTG GACTTAAATA TACTGTGAAA
    CATCGACCAT TTCCTGAAGA GGTGGATAAG ATTCCTTTTG TGAAAGCAGA TCTGAGCATT
    CCAGATTTGC ATGAAATCGT CACGGAAGAA TTAGAAGAGA GACATGAAAA ATTAAGGAAT
    GGCATGGCCC AGCAGATCGC TTATGAAATA CACCTTGAGC AACAAAGTGA GGAGGAATTG
    CAGAAGAGAA GTTTTCCTGA TCCAGTTACA GATTGTAAGC CCCCACCCCC TGCCCAGGAA
    TTCCAAACAG CACGTCTTTT CCTTTCACAC TTCGGATTTT TGTCTTTAGA AGCATTGAAG
    GAACCTGCAA ACAGTCGTCT ACCTCCTCAC CTTATTGCAC TTGACTCCAC GATACCTGGA
    TTTTTTGATG ACATTGGGTA TCTGGATCTC TTGCCATGTC GTCCCTTTGA TACGGTTTTT
    ATTTTCTATA TGAAGCCAGG CCAGAAAACA AACCAGGAGA TTTTAAAGAA CGTGGAGTCT
    TCCAGAAATG TTCAGCCACA TTTCCTAGAA TTTTTGCTCT CCCTTGGCTG GTCAGTAGAT
    GTGGGCAAAC ACCCTGGGTG GACTGGGCAT GTTTCCACCA GTTGGTCTAT TAACAGTTGC
    GATGATGGTG AAGGACCTGA ACAAGATGAA GTGATTTCCT CTGAAGACGT TGGGGCTAGC
    ATTTTCAACG GACAGAAGAA GGTGCTGTAT TATGCTGATG CCCTTACAGA AATAGCTTTT
    GTGGTTCCTT CTCCTGTGGA GTCCTTAACT GATTCATTGG AAAGTAACAT CTCAGACCAA
    GATAGTGATT CAAATATGGA TCTTATGCCA GGAATTCTGA AACAGCCATC CCTCACACTT
    GAGCTTTTCC CTAATCACAC AGACAATCTT AATTCCTCAC AGAGGCTCAG TCCCAGTTCC
    AGAATGAAGA AGCTGCCTCA GGGTCGCCCT GTGCCTCCCC TCGGACCCGA GACAAGAGTT
    TCTGTAGTCT GGGTGGAACG CTATGATGAT ATAGAAAACT TTCCCCTCTC AGACCTGATG
    ACAGAAATCA GTACTGGTGT GGAAACTACT GCAAACAGTA GCACTTCTCT GAGATCTACT
    GCTCTTGAAA AAGAAGTTCC TGTCATCTTC ATCCACCCTT TAAACACTGG ATTATTCCGG
    ATAAAAATTC AAGGAGCCAC TGGAAAATTT AACATGGTCA TCCCTCTCGT GGACGGGATG
    ATTGTCAGTA GGCGAGCGCT CGGTTTTCTG GTGAGGCAGA CTGTAATTAA TATTTGTAGA
    AGAAAGAGAC TGGAGAGTGA CTCCTACAGC CCACCACACG TCCGCCGGAA ACAGAAGATC
    ACTGACATTG TCAACAAGTA CCGGAACAAA CAGTTGGAGC CAGAGTTTTA CACTTCACTT
    TTTCAGGAGG TTGGACTCAA AAACTGCAGT TCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010822520.1
    CDS275..4750
    Translation

    Target ORF information:

    RefSeq Version XM_010824218.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010824218.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC GCTGCATTTG GTGATTCAGA ATGATCAGGG CCACACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTTCATCCT
    CTCGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGGAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACCGATTGGA TTATGGCTTT AGTGTTACCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GACCCGACTA
    TGCTTACAGG TCCTGAAAGC CATTCAAAAA CTGGCCCGTG AGTCATCCAT TATGGCCCGA
    GAAACCTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCGCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCATG TACGCGGTGC TTCCCAACAC CTCCTTACTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCACCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATCTGTGCGC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTTGCA
    CAGACGTGGT TTCGCTTTTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCAGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAACAG ATTGAGTATG CCTCAGAGCA CTGCTGTCAA TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCACACCTC AAAAGTTCAG CATCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCCCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCCGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA GCTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAACTG AGACGATCTT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCACATCA CTTTGGCACA GTCAAATCTG AGGTAAGGTT TTATTAAAGT
    TTTTCTAATG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTTTGTC ACTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAG
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACTTCAAGA GTCACAGTCG CACAAATAGT
    GGTATTAGTT CAGCGAGTGG TGGAAGCACA GAACCCACAA CTCCTGACAG TGAAAGACCT
    GCTCAAGCTC TCCTGAGAGA TTATGATTCA GCTGCTGGGC TCCTGATTCG CAGCATTCAT
    CTCGTCACTC AAAGACTCAA TTCCCAGTGG CGGCAAGACA TGAGCATATC ACTGGCAGCT
    CTGGAGCTCC TCTCTGGACT GGCAAAGGTG AAGGTGATGG TTGACTCTGG AGACCGGAAG
    CGAGCCATCA GTTCTGTGTG CGGCTACATT GTGTACCAGT GTAGTCGGCC AGCTCCTCTT
    CACTCCCGGG ACCTGCACTC CATGATAGTG GCAGCTTTTC AGTGTCTCTG TGTCTGGCTG
    ACAGAACACC CCGACATGCT TGATGAAAAG GATTGCCTTA AGGAAGTGCT GGAAATTGTG
    GAACTGGGTA TTTCAGGAAG CAAGTCCAAG AACAGTGAGC AAGAGGTCAA GTACAAAGGT
    GATAAGGAGC CAAACCCTGC CTCTATGAGA GTAAAGGATG CTGCTGAAGC CACCCTAACA
    TGTATTATGC AGTTGCTTGG CGCATTTCCT TCACCCAGTG GTCCTGCCTC TCCTTGTAGT
    CTGGTGAACG AGACCACTTT GATCAAATAC TCCAGACTGC CAACCATAAA CAAACATAGT
    TTCCGGTACT TTGTCTTGGA TAACAGTGTC ATCCTGGCAA TGCTGGAGCA ACCTCTTGGA
    AATGAACAGA ATGATTTTTT CCCCTCTGTC ACTGTGCTCG TCCGGGGAAT GTCTGGAAGA
    CTTGCTTGGG CACAACAACT TTGCCTTTTA CCCAGAGGAG CAAAAGCAAA TCAGAAGCTT
    TTTGTGCCTG AACCTCGTCC AGTTCCTAAA AATGATGTTG GACTTAAATA TACTGTGAAA
    CATCGACCAT TTCCTGAAGA GGTGGATAAG ATTCCTTTTG TGAAAGCAGA TCTGAGCATT
    CCAGATTTGC ATGAAATCGT CACGGAAGAA TTAGAAGAGA GACATGAAAA ATTAAGGAAT
    GGCATGGCCC AGCAGATCGC TTATGAAATA CACCTTGAGC AACAAAGTGA GGAGGAATTG
    CAGAAGAGAA GTTTTCCTGA TCCAGTTACA GATTGTAAGC CCCCACCCCC TGCCCAGGAA
    TTCCAAACAG CACGTCTTTT CCTTTCACAC TTCGGATTTT TGTCTTTAGA AGCATTGAAG
    GAACCTGCAA ACAGTCGTCT ACCTCCTCAC CTTATTGCAC TTGACTCCAC GATACCTGGA
    TTTTTTGATG ACATTGGGTA TCTGGATCTC TTGCCATGTC GTCCCTTTGA TACGGTTTTT
    ATTTTCTATA TGAAGCCAGG CCAGAAAACA AACCAGGAGA TTTTAAAGAA CGTGGAGTCT
    TCCAGAAATG TTCAGCCACA TTTCCTAGAA TTTTTGCTCT CCCTTGGCTG GTCAGTAGAT
    GTGGGCAAAC ACCCTGGGTG GACTGGGCAT GTTTCCACCA GTTGGTCTAT TAACAGTTGC
    GATGATGGTG AAGGACCTGA ACAAGATGAA GTGATTTCCT CTGAAGACGT TGGGGCTAGC
    ATTTTCAACG GACAGAAGAA GGTGCTGTAT TATGCTGATG CCCTTACAGA AATAGCTTTT
    GTGGTTCCTT CTCCTGTGGA GTCCTTAACT GATTCATTGG AAAGTAACAT CTCAGACCAA
    GATAGTGATT CAAATATGGA TCTTATGCCA GGAATTCTGA AACAGCCATC CCTCACACTT
    GAGCTTTTCC CTAATCACAC AGACAATCTT AATTCCTCAC AGAGGCTCAG TCCCAGTTCC
    AGAATGAAGA AGCTGCCTCA GGGTCGCCCT GTGCCTCCCC TCGGACCCGA GACAAGAGTT
    TCTGTAGTCT GGGTGGAACG CTATGATGAT ATAGAAAACT TTCCCCTCTC AGACCTGATG
    ACAGAAATCA GTACTGGTGT GGAAACTACT GCAAACAGTA GCACTTCTCT GAGATCTACT
    GCTCTTGAAA AAGAAGTTCC TGTCATCTTC ATCCACCCTT TAAACACTGG ATTATTCCGG
    ATAAAAATTC AAGGAGCCAC TGGAAAATTT AACATGGTCA TCCCTCTCGT GGACGGGATG
    ATTGTCAGTA GGCGAGCGCT CGGTTTTCTG GTGAGGCAGA CTGTAATTAA TATTTGTAGA
    AGAAAGAGAC TGGAGAGTGA CTCCTACAGC CCACCACACG TCCGCCGGAA ACAGAAGATC
    ACTGACATTG TCAACAAGTA CCGGAACAAA CAGTTGGAGC CAGAGTTTTA CACTTCACTT
    TTTCAGGAGG TTGGACTCAA AAACTGCAGT TCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.