HIC1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	HIC1
Entrez Gene ID	786191
Full Name	HIC ZBTB transcriptional repressor 1
General protein information	Preferred Names HIC ZBTB transcriptional repressor 1 Names LOW QUALITY PROTEIN: hypermethylated in cancer 1 protein hypermethylated in cancer 1 protein hypermethylated in cancer 1 protein-like
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024980702.1	XP_024836470.1	hypermethylated in cancer 1 protein isoform X1
XM_015467992.1	XP_015323478.1	LOW QUALITY PROTEIN: hypermethylated in cancer 1 protein isoform X2
XM_003587400.4	XP_003587448.2	hypermethylated in cancer 1 protein isoform X1
XM_024980703.1	XP_024836471.1	hypermethylated in cancer 1 protein isoform X2

Pathways from BioSystems

Homologies

	LOC721284	XP_001117386.2
Mus musculus (house mouse)	Hic1	NP_034560.2
Gallus gallus (chicken)	HIC1	NP_990567.1
Xenopus tropicalis (tropical clawed frog)	hic1	XP_004911694.1
Canis lupus familiaris (dog)	HIC1	XP_548318.2
Danio rerio (zebrafish)	hic1	NP_001002314.1
Rattus norvegicus (Norway rat)	LOC100912068	XP_003750910.1
Homo sapiens (human)	HIC1	NP_001091672.1
Bos taurus (cattle)	HIC1	XP_003587448.2

Gene Ontology

The following HIC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa226726	XM_024980702.1 Latest version!	Bos taurus HIC ZBTB transcriptional repressor 1 (HIC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa226727	XM_024980703.1 Latest version!	Bos taurus HIC ZBTB transcriptional repressor 1 (HIC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa29588	XM_003587400.4 Latest version!	Bos taurus hypermethylated in cancer 1 (HIC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa95033	XM_015467992.1 Latest version!	Bos taurus hypermethylated in cancer 1 (HIC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HIC1 ( XM_024980702.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836470.1 Bos taurus HIC ZBTB transcriptional repressor 1 (HIC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa226726
Clone ID Related Accession (Same CDS sequence)	XM_024980702.1
Accession Version	XM_024980702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hypermethylated in cancer 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAT TTAGAAATAT GTACACAGGA ACATTCAGCT CAAAACCCCA ATTTCTGTGG 
GGCTCGCAGG TCCGGGGCTG GGGACTCCAG GTGGGCACAT CTGGAAGGCT GAATCTGAGA 
ACAGGCCCCC CTCCTGGGTC CAGTTCGTCA TCTGCGAGCC TCCATCCCAG GTGCGCAGGA 
AGGAGGCCGT GGCTGTGCCG CGCAAAGTAC CGCACGGCCG CGAGGTGGCG CCATAGCTGC 
AGCAGCGCCC GCCGGCCCGG GCCGCTCCAG ATAAGAGTGT GCGGAAAGCG CGGCGGGGCT 
GAGACGCGAC CAGGACGCGG GGAGGACGGA CCAGCAGGAC AGACCGACCG GGGGCCCGGC 
GGGCGGAGGG CAGCGCAGCG CAGCCACGTC CCCCCCTGGA TCCGCCGGCA GCCGGGCCCG 
GGGCTTCCGA CATGCCCCCC AGGAGAGTGT GCTGGGCAGA CGATGCTGGA CACGATGGAG 
GCGCCCGGCC ACTCCAGGCA GCTGCTGCTG CAGCTCAACA ACCAGCGCAC CAAGGGCTTC 
TTGTGCGACG TGATCATCGT GGTGCAGAAC GCCCTCTTCC GCGCGCACAA GAACGTGCTG 
GCGGCCAGCA GCGCCTACCT CAAGTCCCTG GTGGTGCACG ACAACCTGCT CAACCTGGAC 
CATGACATGG TGAGCCCGGC CGTGTTCCGC CTGGTGCTGG ACTTCATCTA CACCGGCCGC 
CTGGCGGATG GCGCGGAGGC TGCGGCGGCG GCTGCCGTGG CCCCGGGGGC CGAGCCGAGC 
CTGGGCGCCG TGCTGGCCGC CGCCAGCTAC CTGCAGATCC CCGACCTCGT GGCGCTGTGC 
AAGAAACGCC TCAAGCGCCA CGGCAAGTAC TGCCACCTGC GGGGCGGCGG CGGCGGCGGC 
GGCGGCTACG CACCCTACGG GAGGCCGGGC CGGGGCCTGC GGGCCGCCAC GCCCGTCATC 
CAGGCCTGCT ACTCCTCCCC GGCCGGGCCT CCGCCGCCGC CCACCGCCGA GCCGCCGTCG 
GGCCCCGAGG CCGCTGTGAA CACGCACTGC GCCGAGCTGT ACGCCTCGGG CCCCGGCCCG 
GCCACCGCGC TCTGCGCCCC GGAGCGCCGC TGCTCCCCGC TCTGCGGCCT GGACCTGTCA 
AAGAAAAGCC CGCCGGGCTC CGCGCCTCCC GAGCGACCGC CGGGGGAGCG AGAGCTGCCC 
CCACGCCCAG ACAGCCCTCC CAGCGCAGGC CCCGCCGCCT ACAAGGAGCC ACCACTCACC 
CTGCCACCGC TGCCGCCGCT GCCCTTCCAG AAGCTGGAGG AGGCCGGACC GCCTCCGGAT 
CCGTTCCGAG GTGGCGGCGG CAGCCCGGGA TCCGAGCCCC CCGGCCGCCC CGACGGGCCC 
AGCCTCCTCT ACCGCTGGAT GAAGCACGAG CCAGGCCTGG GCAGCTACGG CGACGAGCTG 
GGCCGCGAGC GCGGCTCCCC CAGCGAACGC TGCGAGGAGC GCGGCGGAGA CCCCTCGGCC 
TCGCCCGGGG GGCCTCCGCT CGGCCTGGCG CCGCCGCCGC GCTACGCGGG CAGCTTGGAC 
GGGCCTGGCG CCGGCGGCGA CGGCGACGAC TACAAGAGCA GCAGTGAGGA GACGGGCAGC 
AGTGAGGACC CCAGCCCTCC CGGCGGCCAC CTCGAGGGCT ACCCGTGCCC GCACCTGGCC 
TACGGAGAGC CCGAGAGCTT CGGCGACAAC CTGTACGTTT GCATCCCGTG CGGAAAGGGC 
TTCCCCAGCT CGGAGCAGCT GAACGCGCAC GTGGAGGCGC ACGTGGAGGA GGAGGAGGCG 
CTGTACGGCC GGGCCGAGGC AGCGGAGGTG GCCGCGGGGG CCGCCGGCCT CGGGCCCCCT 
TTTGGAGGCG GTGGGGACAA GGTGGCCGGG GCCCCGGGGG GCCTGGGCGA GCTGCTGCGG 
CCGTACCGCT GCGCGTCGTG CGACAAGAGC TACAAGGACC CGGCCACGCT GCGGCAGCAC 
GAGAAGACCC ACTGGCTGAC GCGGCCCTAC CCCTGCACCA TCTGCGGGAA GAAGTTCACG 
CAGCGCGGGA CCATGACGCG CCACATGCGC AGCCACCTGG GCCTCAAGCC CTTCGCGTGC 
GACGCGTGCG GCATGCGCTT CACGCGCCAG TACCGCCTCA CCGAGCACAT GCGCATCCAC 
TCGGGCGAGA AGCCTTATGA GTGCCAGGTG TGCGGCGGCA AGTTCGCCCA GCAGCGCAAC 
CTCATCAGTC ACATGAAGAT GCACGCTGTG GGCGGCGCGG CCGGCGCGGC CGGGGCGCTG 
GCGGGGCTGG GGGGGCTCCC CGGCGTCCCG GGCCCCGACG GCAAGGGCAA GCTCGATTTC 
CCCGAGGGCG TCTTTGCAGT GGCGCGCCTC ACGGCCGAAC AGCTCAGCCT GAAGCAGCAG 
GACAAGGCGG CTGCGGCCGA GCTGCTGGCT CAGACCACGC ACTTCCTGCA CGACCCCAAG 
GTGGCGCTCG AGAGCCTCTA CCCGCTGGCC AAGTTCACCG CGGAGCTGGG CCTCAGCCCC 
GACAAGGCGG CCGAGGTGCT GAGCCAGGGC GCGCACCTGG CCGCCGGCCC CGACGGCCGA 
ACCATCGACC GTTTCTCTCC CACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836470.1
CDS	2182..4788
Translation	MSEFRNMYTGTFSSKPQFLWGSQVRGWGLQVGTSGRLNLRTGPPPGSSSSSASLHPRCAGRRPWLCRAKYRTAARWRHSCSSARRPGPLQIRVCGKRGGAETRPGRGEDGPAGQTDRGPGGRRAAQRSHVPPWIRRQPGPGLPTCPPGECAGQTMLDTMEAPGHSRQLLLQLNNQRTKGFLCDVIIVVQNALFRAHKNVLAASSAYLKSLVVHDNLLNLDHDMVSPAVFRLVLDFIYTGRLADGAEAAAAAAVAPGAEPSLGAVLAAASYLQIPDLVALCKKRLKRHGKYCHLRGGGGGGGGYAPYGRPGRGLRAATPVIQACYSSPAGPPPPPTAEPPSGPEAAVNTHCAELYASGPGPATALCAPERRCSPLCGLDLSKKSPPGSAPPERPPGERELPPRPDSPPSAGPAAYKEPPLTLPPLPPLPFQKLEEAGPPPDPFRGGGGSPGSEPPGRPDGPSLLYRWMKHEPGLGSYGDELGRERGSPSERCEERGGDPSASPGGPPLGLAPPPRYAGSLDGPGAGGDGDDYKSSSEETGSSEDPSPPGGHLEGYPCPHLAYGEPESFGDNLYVCIPCGKGFPSSEQLNAHVEAHVEEEEALYGRAEAAEVAAGAAGLGPPFGGGGDKVAGAPGGLGELLRPYRCASCDKSYKDPATLRQHEKTHWLTRPYPCTICGKKFTQRGTMTRHMRSHLGLKPFACDACGMRFTRQYRLTEHMRIHSGEKPYECQVCGGKFAQQRNLISHMKMHAVGGAAGAAGALAGLGGLPGVPGPDGKGKLDFPEGVFAVARLTAEQLSLKQQDKAAAAELLAQTTHFLHDPKVALESLYPLAKFTAELGLSPDKAAEVLSQGAHLAAGPDGRTIDRFSPT

Target ORF information:

RefSeq Version	XM_024980702.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HIC ZBTB transcriptional repressor 1 (HIC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980702.1

ORF Insert Sequence:

ATGTCAGAAT TTAGAAATAT GTACACAGGA ACATTCAGCT CAAAACCCCA ATTTCTGTGG 
GGCTCGCAGG TCCGGGGCTG GGGACTCCAG GTGGGCACAT CTGGAAGGCT GAATCTGAGA 
ACAGGCCCCC CTCCTGGGTC CAGTTCGTCA TCTGCGAGCC TCCATCCCAG GTGCGCAGGA 
AGGAGGCCGT GGCTGTGCCG CGCAAAGTAC CGCACGGCCG CGAGGTGGCG CCATAGCTGC 
AGCAGCGCCC GCCGGCCCGG GCCGCTCCAG ATAAGAGTGT GCGGAAAGCG CGGCGGGGCT 
GAGACGCGAC CAGGACGCGG GGAGGACGGA CCAGCAGGAC AGACCGACCG GGGGCCCGGC 
GGGCGGAGGG CAGCGCAGCG CAGCCACGTC CCCCCCTGGA TCCGCCGGCA GCCGGGCCCG 
GGGCTTCCGA CATGCCCCCC AGGAGAGTGT GCTGGGCAGA CGATGCTGGA CACGATGGAG 
GCGCCCGGCC ACTCCAGGCA GCTGCTGCTG CAGCTCAACA ACCAGCGCAC CAAGGGCTTC 
TTGTGCGACG TGATCATCGT GGTGCAGAAC GCCCTCTTCC GCGCGCACAA GAACGTGCTG 
GCGGCCAGCA GCGCCTACCT CAAGTCCCTG GTGGTGCACG ACAACCTGCT CAACCTGGAC 
CATGACATGG TGAGCCCGGC CGTGTTCCGC CTGGTGCTGG ACTTCATCTA CACCGGCCGC 
CTGGCGGATG GCGCGGAGGC TGCGGCGGCG GCTGCCGTGG CCCCGGGGGC CGAGCCGAGC 
CTGGGCGCCG TGCTGGCCGC CGCCAGCTAC CTGCAGATCC CCGACCTCGT GGCGCTGTGC 
AAGAAACGCC TCAAGCGCCA CGGCAAGTAC TGCCACCTGC GGGGCGGCGG CGGCGGCGGC 
GGCGGCTACG CACCCTACGG GAGGCCGGGC CGGGGCCTGC GGGCCGCCAC GCCCGTCATC 
CAGGCCTGCT ACTCCTCCCC GGCCGGGCCT CCGCCGCCGC CCACCGCCGA GCCGCCGTCG 
GGCCCCGAGG CCGCTGTGAA CACGCACTGC GCCGAGCTGT ACGCCTCGGG CCCCGGCCCG 
GCCACCGCGC TCTGCGCCCC GGAGCGCCGC TGCTCCCCGC TCTGCGGCCT GGACCTGTCA 
AAGAAAAGCC CGCCGGGCTC CGCGCCTCCC GAGCGACCGC CGGGGGAGCG AGAGCTGCCC 
CCACGCCCAG ACAGCCCTCC CAGCGCAGGC CCCGCCGCCT ACAAGGAGCC ACCACTCACC 
CTGCCACCGC TGCCGCCGCT GCCCTTCCAG AAGCTGGAGG AGGCCGGACC GCCTCCGGAT 
CCGTTCCGAG GTGGCGGCGG CAGCCCGGGA TCCGAGCCCC CCGGCCGCCC CGACGGGCCC 
AGCCTCCTCT ACCGCTGGAT GAAGCACGAG CCAGGCCTGG GCAGCTACGG CGACGAGCTG 
GGCCGCGAGC GCGGCTCCCC CAGCGAACGC TGCGAGGAGC GCGGCGGAGA CCCCTCGGCC 
TCGCCCGGGG GGCCTCCGCT CGGCCTGGCG CCGCCGCCGC GCTACGCGGG CAGCTTGGAC 
GGGCCTGGCG CCGGCGGCGA CGGCGACGAC TACAAGAGCA GCAGTGAGGA GACGGGCAGC 
AGTGAGGACC CCAGCCCTCC CGGCGGCCAC CTCGAGGGCT ACCCGTGCCC GCACCTGGCC 
TACGGAGAGC CCGAGAGCTT CGGCGACAAC CTGTACGTTT GCATCCCGTG CGGAAAGGGC 
TTCCCCAGCT CGGAGCAGCT GAACGCGCAC GTGGAGGCGC ACGTGGAGGA GGAGGAGGCG 
CTGTACGGCC GGGCCGAGGC AGCGGAGGTG GCCGCGGGGG CCGCCGGCCT CGGGCCCCCT 
TTTGGAGGCG GTGGGGACAA GGTGGCCGGG GCCCCGGGGG GCCTGGGCGA GCTGCTGCGG 
CCGTACCGCT GCGCGTCGTG CGACAAGAGC TACAAGGACC CGGCCACGCT GCGGCAGCAC 
GAGAAGACCC ACTGGCTGAC GCGGCCCTAC CCCTGCACCA TCTGCGGGAA GAAGTTCACG 
CAGCGCGGGA CCATGACGCG CCACATGCGC AGCCACCTGG GCCTCAAGCC CTTCGCGTGC 
GACGCGTGCG GCATGCGCTT CACGCGCCAG TACCGCCTCA CCGAGCACAT GCGCATCCAC 
TCGGGCGAGA AGCCTTATGA GTGCCAGGTG TGCGGCGGCA AGTTCGCCCA GCAGCGCAAC 
CTCATCAGTC ACATGAAGAT GCACGCTGTG GGCGGCGCGG CCGGCGCGGC CGGGGCGCTG 
GCGGGGCTGG GGGGGCTCCC CGGCGTCCCG GGCCCCGACG GCAAGGGCAA GCTCGATTTC 
CCCGAGGGCG TCTTTGCAGT GGCGCGCCTC ACGGCCGAAC AGCTCAGCCT GAAGCAGCAG 
GACAAGGCGG CTGCGGCCGA GCTGCTGGCT CAGACCACGC ACTTCCTGCA CGACCCCAAG 
GTGGCGCTCG AGAGCCTCTA CCCGCTGGCC AAGTTCACCG CGGAGCTGGG CCTCAGCCCC 
GACAAGGCGG CCGAGGTGCT GAGCCAGGGC GCGCACCTGG CCGCCGGCCC CGACGGCCGA 
ACCATCGACC GTTTCTCTCC CACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIC1 ( XM_024980703.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836471.1 Bos taurus HIC ZBTB transcriptional repressor 1 (HIC1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa226727
Clone ID Related Accession (Same CDS sequence)	XM_024980703.1
Accession Version	XM_024980703.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hypermethylated in cancer 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTTTC CTGAAGCGGA CATTTTCCTC AAATCGGGAG AGTGTGCTGG GCAGACGATG 
CTGGACACGA TGGAGGCGCC CGGCCACTCC AGGCAGCTGC TGCTGCAGCT CAACAACCAG 
CGCACCAAGG GCTTCTTGTG CGACGTGATC ATCGTGGTGC AGAACGCCCT CTTCCGCGCG 
CACAAGAACG TGCTGGCGGC CAGCAGCGCC TACCTCAAGT CCCTGGTGGT GCACGACAAC 
CTGCTCAACC TGGACCATGA CATGGTGAGC CCGGCCGTGT TCCGCCTGGT GCTGGACTTC 
ATCTACACCG GCCGCCTGGC GGATGGCGCG GAGGCTGCGG CGGCGGCTGC CGTGGCCCCG 
GGGGCCGAGC CGAGCCTGGG CGCCGTGCTG GCCGCCGCCA GCTACCTGCA GATCCCCGAC 
CTCGTGGCGC TGTGCAAGAA ACGCCTCAAG CGCCACGGCA AGTACTGCCA CCTGCGGGGC 
GGCGGCGGCG GCGGCGGCGG CTACGCACCC TACGGGAGGC CGGGCCGGGG CCTGCGGGCC 
GCCACGCCCG TCATCCAGGC CTGCTACTCC TCCCCGGCCG GGCCTCCGCC GCCGCCCACC 
GCCGAGCCGC CGTCGGGCCC CGAGGCCGCT GTGAACACGC ACTGCGCCGA GCTGTACGCC 
TCGGGCCCCG GCCCGGCCAC CGCGCTCTGC GCCCCGGAGC GCCGCTGCTC CCCGCTCTGC 
GGCCTGGACC TGTCAAAGAA AAGCCCGCCG GGCTCCGCGC CTCCCGAGCG ACCGCCGGGG 
GAGCGAGAGC TGCCCCCACG CCCAGACAGC CCTCCCAGCG CAGGCCCCGC CGCCTACAAG 
GAGCCACCAC TCACCCTGCC ACCGCTGCCG CCGCTGCCCT TCCAGAAGCT GGAGGAGGCC 
GGACCGCCTC CGGATCCGTT CCGAGGTGGC GGCGGCAGCC CGGGATCCGA GCCCCCCGGC 
CGCCCCGACG GGCCCAGCCT CCTCTACCGC TGGATGAAGC ACGAGCCAGG CCTGGGCAGC 
TACGGCGACG AGCTGGGCCG CGAGCGCGGC TCCCCCAGCG AACGCTGCGA GGAGCGCGGC 
GGAGACCCCT CGGCCTCGCC CGGGGGGCCT CCGCTCGGCC TGGCGCCGCC GCCGCGCTAC 
GCGGGCAGCT TGGACGGGCC TGGCGCCGGC GGCGACGGCG ACGACTACAA GAGCAGCAGT 
GAGGAGACGG GCAGCAGTGA GGACCCCAGC CCTCCCGGCG GCCACCTCGA GGGCTACCCG 
TGCCCGCACC TGGCCTACGG AGAGCCCGAG AGCTTCGGCG ACAACCTGTA CGTTTGCATC 
CCGTGCGGAA AGGGCTTCCC CAGCTCGGAG CAGCTGAACG CGCACGTGGA GGCGCACGTG 
GAGGAGGAGG AGGCGCTGTA CGGCCGGGCC GAGGCAGCGG AGGTGGCCGC GGGGGCCGCC 
GGCCTCGGGC CCCCTTTTGG AGGCGGTGGG GACAAGGTGG CCGGGGCCCC GGGGGGCCTG 
GGCGAGCTGC TGCGGCCGTA CCGCTGCGCG TCGTGCGACA AGAGCTACAA GGACCCGGCC 
ACGCTGCGGC AGCACGAGAA GACCCACTGG CTGACGCGGC CCTACCCCTG CACCATCTGC 
GGGAAGAAGT TCACGCAGCG CGGGACCATG ACGCGCCACA TGCGCAGCCA CCTGGGCCTC 
AAGCCCTTCG CGTGCGACGC GTGCGGCATG CGCTTCACGC GCCAGTACCG CCTCACCGAG 
CACATGCGCA TCCACTCGGG CGAGAAGCCT TATGAGTGCC AGGTGTGCGG CGGCAAGTTC 
GCCCAGCAGC GCAACCTCAT CAGTCACATG AAGATGCACG CTGTGGGCGG CGCGGCCGGC 
GCGGCCGGGG CGCTGGCGGG GCTGGGGGGG CTCCCCGGCG TCCCGGGCCC CGACGGCAAG 
GGCAAGCTCG ATTTCCCCGA GGGCGTCTTT GCAGTGGCGC GCCTCACGGC CGAACAGCTC 
AGCCTGAAGC AGCAGGACAA GGCGGCTGCG GCCGAGCTGC TGGCTCAGAC CACGCACTTC 
CTGCACGACC CCAAGGTGGC GCTCGAGAGC CTCTACCCGC TGGCCAAGTT CACCGCGGAG 
CTGGGCCTCA GCCCCGACAA GGCGGCCGAG GTGCTGAGCC AGGGCGCGCA CCTGGCCGCC 
GGCCCCGACG GCCGAACCAT CGACCGTTTC TCTCCCACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836471.1
CDS	177..2378
Translation	MTFPEADIFLKSGECAGQTMLDTMEAPGHSRQLLLQLNNQRTKGFLCDVIIVVQNALFRAHKNVLAASSAYLKSLVVHDNLLNLDHDMVSPAVFRLVLDFIYTGRLADGAEAAAAAAVAPGAEPSLGAVLAAASYLQIPDLVALCKKRLKRHGKYCHLRGGGGGGGGYAPYGRPGRGLRAATPVIQACYSSPAGPPPPPTAEPPSGPEAAVNTHCAELYASGPGPATALCAPERRCSPLCGLDLSKKSPPGSAPPERPPGERELPPRPDSPPSAGPAAYKEPPLTLPPLPPLPFQKLEEAGPPPDPFRGGGGSPGSEPPGRPDGPSLLYRWMKHEPGLGSYGDELGRERGSPSERCEERGGDPSASPGGPPLGLAPPPRYAGSLDGPGAGGDGDDYKSSSEETGSSEDPSPPGGHLEGYPCPHLAYGEPESFGDNLYVCIPCGKGFPSSEQLNAHVEAHVEEEEALYGRAEAAEVAAGAAGLGPPFGGGGDKVAGAPGGLGELLRPYRCASCDKSYKDPATLRQHEKTHWLTRPYPCTICGKKFTQRGTMTRHMRSHLGLKPFACDACGMRFTRQYRLTEHMRIHSGEKPYECQVCGGKFAQQRNLISHMKMHAVGGAAGAAGALAGLGGLPGVPGPDGKGKLDFPEGVFAVARLTAEQLSLKQQDKAAAAELLAQTTHFLHDPKVALESLYPLAKFTAELGLSPDKAAEVLSQGAHLAAGPDGRTIDRFSPT

Target ORF information:

RefSeq Version	XM_024980703.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HIC ZBTB transcriptional repressor 1 (HIC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980703.1

ORF Insert Sequence:

ATGACTTTTC CTGAAGCGGA CATTTTCCTC AAATCGGGAG AGTGTGCTGG GCAGACGATG 
CTGGACACGA TGGAGGCGCC CGGCCACTCC AGGCAGCTGC TGCTGCAGCT CAACAACCAG 
CGCACCAAGG GCTTCTTGTG CGACGTGATC ATCGTGGTGC AGAACGCCCT CTTCCGCGCG 
CACAAGAACG TGCTGGCGGC CAGCAGCGCC TACCTCAAGT CCCTGGTGGT GCACGACAAC 
CTGCTCAACC TGGACCATGA CATGGTGAGC CCGGCCGTGT TCCGCCTGGT GCTGGACTTC 
ATCTACACCG GCCGCCTGGC GGATGGCGCG GAGGCTGCGG CGGCGGCTGC CGTGGCCCCG 
GGGGCCGAGC CGAGCCTGGG CGCCGTGCTG GCCGCCGCCA GCTACCTGCA GATCCCCGAC 
CTCGTGGCGC TGTGCAAGAA ACGCCTCAAG CGCCACGGCA AGTACTGCCA CCTGCGGGGC 
GGCGGCGGCG GCGGCGGCGG CTACGCACCC TACGGGAGGC CGGGCCGGGG CCTGCGGGCC 
GCCACGCCCG TCATCCAGGC CTGCTACTCC TCCCCGGCCG GGCCTCCGCC GCCGCCCACC 
GCCGAGCCGC CGTCGGGCCC CGAGGCCGCT GTGAACACGC ACTGCGCCGA GCTGTACGCC 
TCGGGCCCCG GCCCGGCCAC CGCGCTCTGC GCCCCGGAGC GCCGCTGCTC CCCGCTCTGC 
GGCCTGGACC TGTCAAAGAA AAGCCCGCCG GGCTCCGCGC CTCCCGAGCG ACCGCCGGGG 
GAGCGAGAGC TGCCCCCACG CCCAGACAGC CCTCCCAGCG CAGGCCCCGC CGCCTACAAG 
GAGCCACCAC TCACCCTGCC ACCGCTGCCG CCGCTGCCCT TCCAGAAGCT GGAGGAGGCC 
GGACCGCCTC CGGATCCGTT CCGAGGTGGC GGCGGCAGCC CGGGATCCGA GCCCCCCGGC 
CGCCCCGACG GGCCCAGCCT CCTCTACCGC TGGATGAAGC ACGAGCCAGG CCTGGGCAGC 
TACGGCGACG AGCTGGGCCG CGAGCGCGGC TCCCCCAGCG AACGCTGCGA GGAGCGCGGC 
GGAGACCCCT CGGCCTCGCC CGGGGGGCCT CCGCTCGGCC TGGCGCCGCC GCCGCGCTAC 
GCGGGCAGCT TGGACGGGCC TGGCGCCGGC GGCGACGGCG ACGACTACAA GAGCAGCAGT 
GAGGAGACGG GCAGCAGTGA GGACCCCAGC CCTCCCGGCG GCCACCTCGA GGGCTACCCG 
TGCCCGCACC TGGCCTACGG AGAGCCCGAG AGCTTCGGCG ACAACCTGTA CGTTTGCATC 
CCGTGCGGAA AGGGCTTCCC CAGCTCGGAG CAGCTGAACG CGCACGTGGA GGCGCACGTG 
GAGGAGGAGG AGGCGCTGTA CGGCCGGGCC GAGGCAGCGG AGGTGGCCGC GGGGGCCGCC 
GGCCTCGGGC CCCCTTTTGG AGGCGGTGGG GACAAGGTGG CCGGGGCCCC GGGGGGCCTG 
GGCGAGCTGC TGCGGCCGTA CCGCTGCGCG TCGTGCGACA AGAGCTACAA GGACCCGGCC 
ACGCTGCGGC AGCACGAGAA GACCCACTGG CTGACGCGGC CCTACCCCTG CACCATCTGC 
GGGAAGAAGT TCACGCAGCG CGGGACCATG ACGCGCCACA TGCGCAGCCA CCTGGGCCTC 
AAGCCCTTCG CGTGCGACGC GTGCGGCATG CGCTTCACGC GCCAGTACCG CCTCACCGAG 
CACATGCGCA TCCACTCGGG CGAGAAGCCT TATGAGTGCC AGGTGTGCGG CGGCAAGTTC 
GCCCAGCAGC GCAACCTCAT CAGTCACATG AAGATGCACG CTGTGGGCGG CGCGGCCGGC 
GCGGCCGGGG CGCTGGCGGG GCTGGGGGGG CTCCCCGGCG TCCCGGGCCC CGACGGCAAG 
GGCAAGCTCG ATTTCCCCGA GGGCGTCTTT GCAGTGGCGC GCCTCACGGC CGAACAGCTC 
AGCCTGAAGC AGCAGGACAA GGCGGCTGCG GCCGAGCTGC TGGCTCAGAC CACGCACTTC 
CTGCACGACC CCAAGGTGGC GCTCGAGAGC CTCTACCCGC TGGCCAAGTT CACCGCGGAG 
CTGGGCCTCA GCCCCGACAA GGCGGCCGAG GTGCTGAGCC AGGGCGCGCA CCTGGCCGCC 
GGCCCCGACG GCCGAACCAT CGACCGTTTC TCTCCCACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIC1 ( XM_003587400.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587448.2 Bos taurus hypermethylated in cancer 1 (HIC1), transcript variant X1, mRNA.

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CloneID	OBa29588
Clone ID Related Accession (Same CDS sequence)	XM_003587400.4
Accession Version	XM_003587400.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hypermethylated in cancer 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587400.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAT TTAGAAATAT GTACACAGGA ACATTCAGCT CAAAACCCCA ATTTCTGTGG 
GGCTCGCAGG TCCGGGGCTG GGGACTCCAG GTGGGCACAT CTGGAAGGCT GAATCTGAGA 
ACAGGCCCCC CTCCTGGGTC CAGTTCGTCA TCTGCGAGCC TCCATCCCAG GTGCGCAGGA 
AGGAGGCCGT GGCTGTGCCG CGCAAAGTAC CGCACGGCCG CGAGGTGGCG CCATAGCTGC 
AGCAGCGCCC GCCGGCCCGG GCCGCTCCAG ATAAGAGTGT GCGGAAAGCG CGGCGGGGCT 
GAGACGCGAC CAGGACGCGG GGAGGACGGA CCAGCAGGAC AGACCGACCG GGGGCCCGGC 
GGGCGGAGGG CAGCGCAGCG CAGCCACGTC CCCCCCTGGA TCCGCCGGCA GCCGGGCCCG 
GGGCTTCCGA CATGCCCCCC AGGAGAGTGT GCTGGGCAGA CGATGCTGGA CACGATGGAG 
GCGCCCGGCC ACTCCAGGCA GCTGCTGCTG CAGCTCAACA ACCAGCGCAC CAAGGGCTTC 
TTGTGCGACG TGATCATCGT GGTGCAGAAC GCCCTCTTCC GCGCGCACAA GAACGTGCTG 
GCGGCCAGCA GCGCCTACCT CAAGTCCCTG GTGGTGCACG ACAACCTGCT CAACCTGGAC 
CATGACATGG TGAGCCCGGC CGTGTTCCGC CTGGTGCTGG ACTTCATCTA CACCGGCCGC 
CTGGCGGATG GCGCGGAGGC TGCGGCGGCG GCTGCCGTGG CCCCGGGGGC CGAGCCGAGC 
CTGGGCGCCG TGCTGGCCGC CGCCAGCTAC CTGCAGATCC CCGACCTCGT GGCGCTGTGC 
AAGAAACGCC TCAAGCGCCA CGGCAAGTAC TGCCACCTGC GGGGCGGCGG CGCACGCCCA 
GACAGCCCTC CCAGCGCAGG CCCCGCCGCC TACAAGGAGC CACCACTCAC CCTGCCACCG 
CTGCCGCCGC TGCCCTTCCA GAAGCTGGAG GAGGCCGGAC CGCCTCCGGA TCCGTTCCGA 
GGTGGCGGCG GCAGCCCGGG ATCCGAGCCC CCCGGCCGCC CCGACGGGCC CAGCCTCCTC 
TACCGCTGGA TGAAGCACGA GCCAGGCCTG GGCAGCTACG GCGACGAGCT GGGCCGCGAG 
CGCGGCTCCC CCAGCGAACG CTGCGAGGAG CGCGGCGGAG ACCCCTCGGC CTCGCCCGGG 
GGGCCTCCGC TCGGCCTGGC GCCGCCGCCG CGCTACGCGG GCAGCTTGGA CGGGCCTGGC 
GCCGGCGGCG ACGGCGACGA CTACAAGAGC AGCAGTGAGG AGACGGGCAG CAGTGAGGAC 
CCCAGCCCTC CCGGCGGCCA CCTCGAGGGC TACCCGTGCC CGCACCTGGC CTACGGAGAG 
CCCGAGAGCT TCGGCGACAA CCTGTACGTT TGCATCCCGT GCGGAAAGGG CTTCCCCAGC 
TCGGAGCAGC TGAACGCGCA CGTGGAGGCG CACGTGGAGG AGGAGGAGGC GCTGTACGGC 
CGGGCCGAGG CAGCGGAGGT GGCCGCGGGG GCCGCCGGCC TCGGGCCCCC TTTTGGAGGC 
GGTGGGGACA AGGTGGCCGG GGCCCCGGGG GGCCTGGGCG AGCTGCTGCG GCCGTACCGC 
TGCGCGTCGT GCGACAAGAG CTACAAGGAC CCGGCCACGC TGCGGCAGCA CGAGAAGACC 
CACTGGCTGA CGCGGCCCTA CCCCTGCACC ATCTGCGGGA AGAAGTTCAC GCAGCGCGGG 
ACCATGACGC GCCACATGCG CAGCCACCTG GGCCTCAAGC CCTTCGCGTG CGACGCGTGC 
GGCATGCGCT TCACGCGCCA GTACCGCCTC ACCGAGCACA TGCGCATCCA CTCGGGCGAG 
AAGCCTTATG AGTGCCAGGT GTGCGGCGGC AAGTTCGCCC AGCAGCGCAA CCTCATCAGT 
CACATGAAGA TGCACGCTGT GGGCGGCGCG GCCGGCGCGG CCGGGGCGCT GGCGGGGCTG 
GGGGGGCTCC CCGGCGTCCC GGGCCCCGAC GGCAAGGGCA AGCTCGATTT CCCCGAGGGC 
GTCTTTGCAG TGGCGCGCCT CACGGCCGAA CAGCTCAGCC TGAAGCAGCA GGACAAGGCG 
GCTGCGGCCG AGCTGCTGGC TCAGACCACG CACTTCCTGC ACGACCCCAA GGTGGCGCTC 
GAGAGCCTCT ACCCGCTGGC CAAGTTCACC GCGGAGCTGG GCCTCAGCCC CGACAAGGCG 
GCCGAGGTGC TGAGCCAGGG CGCGCACCTG GCCGCCGGCC CCGACGGCCG AACCATCGAC 
CGTTTCTCTC CCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587448.2
CDS	2420..4717
Translation	MSEFRNMYTGTFSSKPQFLWGSQVRGWGLQVGTSGRLNLRTGPPPGSSSSSASLHPRCAGRRPWLCRAKYRTAARWRHSCSSARRPGPLQIRVCGKRGGAETRPGRGEDGPAGQTDRGPGGRRAAQRSHVPPWIRRQPGPGLPTCPPGECAGQTMLDTMEAPGHSRQLLLQLNNQRTKGFLCDVIIVVQNALFRAHKNVLAASSAYLKSLVVHDNLLNLDHDMVSPAVFRLVLDFIYTGRLADGAEAAAAAAVAPGAEPSLGAVLAAASYLQIPDLVALCKKRLKRHGKYCHLRGGGARPDSPPSAGPAAYKEPPLTLPPLPPLPFQKLEEAGPPPDPFRGGGGSPGSEPPGRPDGPSLLYRWMKHEPGLGSYGDELGRERGSPSERCEERGGDPSASPGGPPLGLAPPPRYAGSLDGPGAGGDGDDYKSSSEETGSSEDPSPPGGHLEGYPCPHLAYGEPESFGDNLYVCIPCGKGFPSSEQLNAHVEAHVEEEEALYGRAEAAEVAAGAAGLGPPFGGGGDKVAGAPGGLGELLRPYRCASCDKSYKDPATLRQHEKTHWLTRPYPCTICGKKFTQRGTMTRHMRSHLGLKPFACDACGMRFTRQYRLTEHMRIHSGEKPYECQVCGGKFAQQRNLISHMKMHAVGGAAGAAGALAGLGGLPGVPGPDGKGKLDFPEGVFAVARLTAEQLSLKQQDKAAAAELLAQTTHFLHDPKVALESLYPLAKFTAELGLSPDKAAEVLSQGAHLAAGPDGRTIDRFSPT

Target ORF information:

RefSeq Version	XM_003587400.4
Organism	Bos taurus(cattle)
Definition	Bos taurus hypermethylated in cancer 1 (HIC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587400.4

ORF Insert Sequence:

ATGTCAGAAT TTAGAAATAT GTACACAGGA ACATTCAGCT CAAAACCCCA ATTTCTGTGG 
GGCTCGCAGG TCCGGGGCTG GGGACTCCAG GTGGGCACAT CTGGAAGGCT GAATCTGAGA 
ACAGGCCCCC CTCCTGGGTC CAGTTCGTCA TCTGCGAGCC TCCATCCCAG GTGCGCAGGA 
AGGAGGCCGT GGCTGTGCCG CGCAAAGTAC CGCACGGCCG CGAGGTGGCG CCATAGCTGC 
AGCAGCGCCC GCCGGCCCGG GCCGCTCCAG ATAAGAGTGT GCGGAAAGCG CGGCGGGGCT 
GAGACGCGAC CAGGACGCGG GGAGGACGGA CCAGCAGGAC AGACCGACCG GGGGCCCGGC 
GGGCGGAGGG CAGCGCAGCG CAGCCACGTC CCCCCCTGGA TCCGCCGGCA GCCGGGCCCG 
GGGCTTCCGA CATGCCCCCC AGGAGAGTGT GCTGGGCAGA CGATGCTGGA CACGATGGAG 
GCGCCCGGCC ACTCCAGGCA GCTGCTGCTG CAGCTCAACA ACCAGCGCAC CAAGGGCTTC 
TTGTGCGACG TGATCATCGT GGTGCAGAAC GCCCTCTTCC GCGCGCACAA GAACGTGCTG 
GCGGCCAGCA GCGCCTACCT CAAGTCCCTG GTGGTGCACG ACAACCTGCT CAACCTGGAC 
CATGACATGG TGAGCCCGGC CGTGTTCCGC CTGGTGCTGG ACTTCATCTA CACCGGCCGC 
CTGGCGGATG GCGCGGAGGC TGCGGCGGCG GCTGCCGTGG CCCCGGGGGC CGAGCCGAGC 
CTGGGCGCCG TGCTGGCCGC CGCCAGCTAC CTGCAGATCC CCGACCTCGT GGCGCTGTGC 
AAGAAACGCC TCAAGCGCCA CGGCAAGTAC TGCCACCTGC GGGGCGGCGG CGCACGCCCA 
GACAGCCCTC CCAGCGCAGG CCCCGCCGCC TACAAGGAGC CACCACTCAC CCTGCCACCG 
CTGCCGCCGC TGCCCTTCCA GAAGCTGGAG GAGGCCGGAC CGCCTCCGGA TCCGTTCCGA 
GGTGGCGGCG GCAGCCCGGG ATCCGAGCCC CCCGGCCGCC CCGACGGGCC CAGCCTCCTC 
TACCGCTGGA TGAAGCACGA GCCAGGCCTG GGCAGCTACG GCGACGAGCT GGGCCGCGAG 
CGCGGCTCCC CCAGCGAACG CTGCGAGGAG CGCGGCGGAG ACCCCTCGGC CTCGCCCGGG 
GGGCCTCCGC TCGGCCTGGC GCCGCCGCCG CGCTACGCGG GCAGCTTGGA CGGGCCTGGC 
GCCGGCGGCG ACGGCGACGA CTACAAGAGC AGCAGTGAGG AGACGGGCAG CAGTGAGGAC 
CCCAGCCCTC CCGGCGGCCA CCTCGAGGGC TACCCGTGCC CGCACCTGGC CTACGGAGAG 
CCCGAGAGCT TCGGCGACAA CCTGTACGTT TGCATCCCGT GCGGAAAGGG CTTCCCCAGC 
TCGGAGCAGC TGAACGCGCA CGTGGAGGCG CACGTGGAGG AGGAGGAGGC GCTGTACGGC 
CGGGCCGAGG CAGCGGAGGT GGCCGCGGGG GCCGCCGGCC TCGGGCCCCC TTTTGGAGGC 
GGTGGGGACA AGGTGGCCGG GGCCCCGGGG GGCCTGGGCG AGCTGCTGCG GCCGTACCGC 
TGCGCGTCGT GCGACAAGAG CTACAAGGAC CCGGCCACGC TGCGGCAGCA CGAGAAGACC 
CACTGGCTGA CGCGGCCCTA CCCCTGCACC ATCTGCGGGA AGAAGTTCAC GCAGCGCGGG 
ACCATGACGC GCCACATGCG CAGCCACCTG GGCCTCAAGC CCTTCGCGTG CGACGCGTGC 
GGCATGCGCT TCACGCGCCA GTACCGCCTC ACCGAGCACA TGCGCATCCA CTCGGGCGAG 
AAGCCTTATG AGTGCCAGGT GTGCGGCGGC AAGTTCGCCC AGCAGCGCAA CCTCATCAGT 
CACATGAAGA TGCACGCTGT GGGCGGCGCG GCCGGCGCGG CCGGGGCGCT GGCGGGGCTG 
GGGGGGCTCC CCGGCGTCCC GGGCCCCGAC GGCAAGGGCA AGCTCGATTT CCCCGAGGGC 
GTCTTTGCAG TGGCGCGCCT CACGGCCGAA CAGCTCAGCC TGAAGCAGCA GGACAAGGCG 
GCTGCGGCCG AGCTGCTGGC TCAGACCACG CACTTCCTGC ACGACCCCAA GGTGGCGCTC 
GAGAGCCTCT ACCCGCTGGC CAAGTTCACC GCGGAGCTGG GCCTCAGCCC CGACAAGGCG 
GCCGAGGTGC TGAGCCAGGG CGCGCACCTG GCCGCCGGCC CCGACGGCCG AACCATCGAC 
CGTTTCTCTC CCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIC1 ( XM_015467992.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323478.1 Bos taurus hypermethylated in cancer 1 (HIC1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa95033
Clone ID Related Accession (Same CDS sequence)	XM_015467992.1
Accession Version	XM_015467992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LOW QUALITY PROTEIN: hypermethylated in cancer 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 4 indels ##RefSeq-Attributes-END##

ATGTCAGAAT TTAGAAATAT GTACACAGGA ACATTCAGCT CAAAACCCCA ATTTCTGTGG 
GGCTCGCAGG TCCGGGGCTG GGGACTCCAG GTGGGCACAT CTGGAAGGCT GAATCTGAGA 
ACAGGCCCCC CTCCTGGGTC CAGTTCGTCA TCTGCGAGCC TCCATCCCAG GTGCGCAGGA 
AGGAGGCCGT GGCTGTGCCG CGCAAAGTAC CGCACGGCCG CGAGGTGGCG CCATAGCTGC 
AGCAGCGCCC GCCGGCCCGG GCCGCTCCAG ATAAGAGTGT GCGGAAAGCG CGGCGGGGCT 
GAGACGCGAC CAGGACGCGG GGAGGACGGA CCAGCAGGAC AGACCGACCG GGGGCCCGGC 
GGGCGGAGGG CAGCGCAGCG CAGCCACGTC CCCCCCTGGA TCCGCCGGCA GCCGGGCCCG 
GGGCTTCCGA CATGCCCCCC AGGAGAGTGT GCTGGGCAGA CGATGCTGGA CACGATGGAG 
GCGCCCGGCC ACTCCAGGCA GCTGCTGCTG CAGCTCAACA ACCAGCGCAC CAAGGGCTTC 
TTGTGCGACG TGATCATCGT GGTGCAGAAC GCCCTCTTCC GCGCGCACAA GAACGTGCTG 
GCGGCCAGCA GCGCCTACCT CAAGTCCCTG GTGGTGCACG ACAACCTGCT CAACCTGGAC 
CATGACATGG TGAGCCCGGC CGTGTTCCGC CTGGTGCTGG ACTTCATCTA CACCGGCCGC 
CTGGCGGATG GCGCGGAGGC TGCGGCGGCG GCTGCCGTGG CCCCGGGGGC CGAGCCGAGC 
CTGGGCGCCG TGCTGGCCGC CGCCAGCTAC CTGCAGATCC CCGACCTCGT GGCGCTGTGC 
AAGAAACGCC TCAAGCGCCA CGGCAAGTAC TGCCACCTGC GGGGCCCCGG CACGGCCGGG 
CGGGGCGGCG GCTACGCACC CTACGGGAGG CCGGGCCGGG GCCTGCGGGC CGCCACGCCC 
GTCATCCAGG CCTGCTACTC CTCCGGCCGG CCTCCGCGCC GCCACCCCGA GCCGCCGNTC 
GGGCCCGAGG CCGCTGTGAA CACGCACTGC GCCGAGCTGT ACGCCTCGGG CCCCGGCCCG 
GCCACCGCGC TCTGCGCCCC GGAGCGCCGC TGCTCCCCGC TCTGCGGCCT GGACCTGTCA 
AAGAAAAGCC CGCCGGGCTC CGCGCCTCCC GAGCGACCGC CGGGGGAGCG AGGAGAGCTG 
CCCCCACGCC CAGACAGCCC TCCCAGCGCA GGCCCCGCCG CCTACAAGGA GCCACCACTC 
ACCCTGCCAC CGCTGCCGCC GCTGCCCTTC CAGAAGCTGG AGGAGGCCGG ACCGCCTCCG 
GATCCGTTCC GAGGTGGCGG CGGCAGCCCG GGATCCGAGC CCCCCGGCCG CCCCGACGGG 
CCCAGCCTCC TCTACCGCTG GATGAAGCAC GAGCCAGGCC TGGGCAGCTA CGGCGACGAG 
CTGGGCCGCG AGCGCGGCTC CCCCAGCGAA CGCTGCGAGG AGCGCGGCGG AGACCCCTCG 
GCCTCGCCCG GGGGGCCTCC GCTCGGCCTG GCGCCGCCGC CGCGCTACGC GGGCAGCTTG 
GACGGGCCTG GCGCCGGCGG CGACGGCGAC GACTACAAGA GCAGCAGTGA GGAGACGGGC 
AGCAGTGAGG ACCCCAGCCC TCCCGGCGGC CACCTCGAGG GCTACCCGTG CCCGCACCTG 
GCCTACGGAG AGCCCGAGAG CTTCGGCGAC AACCTGTACG TTTGCATCCC GTGCGGAAAG 
GGCTTCCCCA GCTCGGAGCA GCTGAACGCG CACGTGGAGG CGCACGTGGA GGAGGAGGAG 
GCGCTGTACG GCCGGGCCGA GGCAGCGGAG GTGGCCGCGG GGGCCGCCGG CCTCGGGCCC 
CCTTTTGGAG GCGGTGGGGA CAAGGTGGCC GGGGCCCCGG GGGGCCTGGG CGAGCTGCTG 
CGGCCGTACC GCTGCGCGTC GTGCGACAAG AGCTACAAGG ACCCGGCCAC GCTGCGGCAG 
CACGAGAAGA CCCACTGGCT GACGCGGCCC TACCCCTGCA CCATCTGCGG GAAGAAGTTC 
ACGCAGCGCG GGACCATGAC GCGCCACATG CGCAGCCACC TGGGCCTCAA GCCCTTCGCG 
TGCGACGCGT GCGGCATGCG CTTCACGCGC CAGTACCGCC TCACCGAGCA CATGCGCATC 
CACTCGGGCG AGAAGCCTTA TGAGTGCCAG GTGTGCGGCG GCAAGTTCGC CCAGCAGCGC 
AACCTCATCA GTCACATGAA GATGCACGCT GTGGGCGGCG CGGCCGGCGC GGCCGGGGCG 
CTGGCGGGGC TGGGGGGGCT CCCCGGCGTC CCGGGCCCCG ACGGCAAGGG CAAGCTCGAT 
TTCCCCGAGG GCGTCTTTGC AGTGGCGCGC CTCACGGCCG AACAGCTCAG CCTGAAGCAG 
CAGGACAAGG CGGCTGCGGC CGAGCTGCTG GCTCAGACCA CGCACTTCCT GCACGACCCC 
AAGGTGGCGC TCGAGAGCCT CTACCCGCTG GCCAAGTTCA CCGCGGAGCT GGGCCTCAGC 
CCCGACAAGG CGGCCGAGGT GCTGAGCCAG GGCGCGCACC TGGCCGCCGG CCCCGACGGC 
CGAACCATCG ACCGTTTCTC TCCCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323478.1
CDS	2232..4841
Translation	MSEFRNMYTGTFSSKPQFLWGSQVRGWGLQVGTSGRLNLRTGPPPGSSSSSASLHPRCAGRRPWLCRAKYRTAARWRHSCSSARRPGPLQIRVCGKRGGAETRPGRGEDGPAGQTDRGPGGRRAAQRSHVPPWIRRQPGPGLPTCPPGECAGQTMLDTMEAPGHSRQLLLQLNNQRTKGFLCDVIIVVQNALFRAHKNVLAASSAYLKSLVVHDNLLNLDHDMVSPAVFRLVLDFIYTGRLADGAEAAAAAAVAPGAEPSLGAVLAAASYLQIPDLVALCKKRLKRHGKYCHLRGPGTAGRGGGYAPYGRPGRGLRAATPVIQACYSSGRPPRRHPEPPnullGPEAAVNTHCAELYASGPGPATALCAPERRCSPLCGLDLSKKSPPGSAPPERPPGERGELPPRPDSPPSAGPAAYKEPPLTLPPLPPLPFQKLEEAGPPPDPFRGGGGSPGSEPPGRPDGPSLLYRWMKHEPGLGSYGDELGRERGSPSERCEERGGDPSASPGGPPLGLAPPPRYAGSLDGPGAGGDGDDYKSSSEETGSSEDPSPPGGHLEGYPCPHLAYGEPESFGDNLYVCIPCGKGFPSSEQLNAHVEAHVEEEEALYGRAEAAEVAAGAAGLGPPFGGGGDKVAGAPGGLGELLRPYRCASCDKSYKDPATLRQHEKTHWLTRPYPCTICGKKFTQRGTMTRHMRSHLGLKPFACDACGMRFTRQYRLTEHMRIHSGEKPYECQVCGGKFAQQRNLISHMKMHAVGGAAGAAGALAGLGGLPGVPGPDGKGKLDFPEGVFAVARLTAEQLSLKQQDKAAAAELLAQTTHFLHDPKVALESLYPLAKFTAELGLSPDKAAEVLSQGAHLAAGPDGRTIDRFSPT

Target ORF information:

RefSeq Version	XM_015467992.1
Organism	Bos taurus(cattle)
Definition	Bos taurus hypermethylated in cancer 1 (HIC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015467992.1

ORF Insert Sequence:

ATGTCAGAAT TTAGAAATAT GTACACAGGA ACATTCAGCT CAAAACCCCA ATTTCTGTGG 
GGCTCGCAGG TCCGGGGCTG GGGACTCCAG GTGGGCACAT CTGGAAGGCT GAATCTGAGA 
ACAGGCCCCC CTCCTGGGTC CAGTTCGTCA TCTGCGAGCC TCCATCCCAG GTGCGCAGGA 
AGGAGGCCGT GGCTGTGCCG CGCAAAGTAC CGCACGGCCG CGAGGTGGCG CCATAGCTGC 
AGCAGCGCCC GCCGGCCCGG GCCGCTCCAG ATAAGAGTGT GCGGAAAGCG CGGCGGGGCT 
GAGACGCGAC CAGGACGCGG GGAGGACGGA CCAGCAGGAC AGACCGACCG GGGGCCCGGC 
GGGCGGAGGG CAGCGCAGCG CAGCCACGTC CCCCCCTGGA TCCGCCGGCA GCCGGGCCCG 
GGGCTTCCGA CATGCCCCCC AGGAGAGTGT GCTGGGCAGA CGATGCTGGA CACGATGGAG 
GCGCCCGGCC ACTCCAGGCA GCTGCTGCTG CAGCTCAACA ACCAGCGCAC CAAGGGCTTC 
TTGTGCGACG TGATCATCGT GGTGCAGAAC GCCCTCTTCC GCGCGCACAA GAACGTGCTG 
GCGGCCAGCA GCGCCTACCT CAAGTCCCTG GTGGTGCACG ACAACCTGCT CAACCTGGAC 
CATGACATGG TGAGCCCGGC CGTGTTCCGC CTGGTGCTGG ACTTCATCTA CACCGGCCGC 
CTGGCGGATG GCGCGGAGGC TGCGGCGGCG GCTGCCGTGG CCCCGGGGGC CGAGCCGAGC 
CTGGGCGCCG TGCTGGCCGC CGCCAGCTAC CTGCAGATCC CCGACCTCGT GGCGCTGTGC 
AAGAAACGCC TCAAGCGCCA CGGCAAGTAC TGCCACCTGC GGGGCCCCGG CACGGCCGGG 
CGGGGCGGCG GCTACGCACC CTACGGGAGG CCGGGCCGGG GCCTGCGGGC CGCCACGCCC 
GTCATCCAGG CCTGCTACTC CTCCGGCCGG CCTCCGCGCC GCCACCCCGA GCCGCCGNTC 
GGGCCCGAGG CCGCTGTGAA CACGCACTGC GCCGAGCTGT ACGCCTCGGG CCCCGGCCCG 
GCCACCGCGC TCTGCGCCCC GGAGCGCCGC TGCTCCCCGC TCTGCGGCCT GGACCTGTCA 
AAGAAAAGCC CGCCGGGCTC CGCGCCTCCC GAGCGACCGC CGGGGGAGCG AGGAGAGCTG 
CCCCCACGCC CAGACAGCCC TCCCAGCGCA GGCCCCGCCG CCTACAAGGA GCCACCACTC 
ACCCTGCCAC CGCTGCCGCC GCTGCCCTTC CAGAAGCTGG AGGAGGCCGG ACCGCCTCCG 
GATCCGTTCC GAGGTGGCGG CGGCAGCCCG GGATCCGAGC CCCCCGGCCG CCCCGACGGG 
CCCAGCCTCC TCTACCGCTG GATGAAGCAC GAGCCAGGCC TGGGCAGCTA CGGCGACGAG 
CTGGGCCGCG AGCGCGGCTC CCCCAGCGAA CGCTGCGAGG AGCGCGGCGG AGACCCCTCG 
GCCTCGCCCG GGGGGCCTCC GCTCGGCCTG GCGCCGCCGC CGCGCTACGC GGGCAGCTTG 
GACGGGCCTG GCGCCGGCGG CGACGGCGAC GACTACAAGA GCAGCAGTGA GGAGACGGGC 
AGCAGTGAGG ACCCCAGCCC TCCCGGCGGC CACCTCGAGG GCTACCCGTG CCCGCACCTG 
GCCTACGGAG AGCCCGAGAG CTTCGGCGAC AACCTGTACG TTTGCATCCC GTGCGGAAAG 
GGCTTCCCCA GCTCGGAGCA GCTGAACGCG CACGTGGAGG CGCACGTGGA GGAGGAGGAG 
GCGCTGTACG GCCGGGCCGA GGCAGCGGAG GTGGCCGCGG GGGCCGCCGG CCTCGGGCCC 
CCTTTTGGAG GCGGTGGGGA CAAGGTGGCC GGGGCCCCGG GGGGCCTGGG CGAGCTGCTG 
CGGCCGTACC GCTGCGCGTC GTGCGACAAG AGCTACAAGG ACCCGGCCAC GCTGCGGCAG 
CACGAGAAGA CCCACTGGCT GACGCGGCCC TACCCCTGCA CCATCTGCGG GAAGAAGTTC 
ACGCAGCGCG GGACCATGAC GCGCCACATG CGCAGCCACC TGGGCCTCAA GCCCTTCGCG 
TGCGACGCGT GCGGCATGCG CTTCACGCGC CAGTACCGCC TCACCGAGCA CATGCGCATC 
CACTCGGGCG AGAAGCCTTA TGAGTGCCAG GTGTGCGGCG GCAAGTTCGC CCAGCAGCGC 
AACCTCATCA GTCACATGAA GATGCACGCT GTGGGCGGCG CGGCCGGCGC GGCCGGGGCG 
CTGGCGGGGC TGGGGGGGCT CCCCGGCGTC CCGGGCCCCG ACGGCAAGGG CAAGCTCGAT 
TTCCCCGAGG GCGTCTTTGC AGTGGCGCGC CTCACGGCCG AACAGCTCAG CCTGAAGCAG 
CAGGACAAGG CGGCTGCGGC CGAGCTGCTG GCTCAGACCA CGCACTTCCT GCACGACCCC 
AAGGTGGCGC TCGAGAGCCT CTACCCGCTG GCCAAGTTCA CCGCGGAGCT GGGCCTCAGC 
CCCGACAAGG CGGCCGAGGT GCTGAGCCAG GGCGCGCACC TGGCCGCCGG CCCCGACGGC 
CGAACCATCG ACCGTTTCTC TCCCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HIC1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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電話

FAX

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