SEMA4D cDNA ORF clone, Bos taurus(cattle)

The following SEMA4D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA4D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa37170 NM_001098160.1
Latest version!
Bos taurus semaphorin 4D (SEMA4D), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa22316 XM_005210464.4
Latest version!
Bos taurus semaphorin 4D (SEMA4D), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa22316 XM_015472658.1
Latest version!
Bos taurus semaphorin 4D (SEMA4D), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa22316 XM_005210463.4
Latest version!
Bos taurus semaphorin 4D (SEMA4D), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa22316 XM_024996004.1
Latest version!
Bos taurus semaphorin 4D (SEMA4D), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa22316 XM_005210466.3
Latest version!
Bos taurus semaphorin 4D (SEMA4D), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa22316 XM_015472659.1
Latest version!
Bos taurus semaphorin 4D (SEMA4D), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa237302 XM_024996006.1
Latest version!
Bos taurus semaphorin 4D (SEMA4D), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa237302 XM_024996005.1
Latest version!
Bos taurus semaphorin 4D (SEMA4D), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OBa22316 XM_005210463.3 Bos taurus semaphorin 4D (SEMA4D), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa22316 XM_005210464.3 Bos taurus semaphorin 4D (SEMA4D), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa22316 XM_005210466.2 Bos taurus semaphorin 4D (SEMA4D), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa37170
    Clone ID Related Accession (Same CDS sequence) NM_001098160.1
    Accession Version NM_001098160.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product semaphorin-4D precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC140509.1. On May 24, 2007 this sequence version replaced XM_001253790.1. ##Evidence-Data-START## Transcript exon combination :: BC140509.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145418 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCCGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGCCTCATC TCCTCTGCCC
    TTCCCTCCTC CGGGGTCCTC TTCCCTGTCC TGTCTGGGCC CCGACTCATC TTCCTCTCCC
    CGAAGCCCCT GGCCGGCCTC GGGCCCAGGG CCTGACCGCC GCCTGCAGGT CACCTTGCTG
    CCGCCCTTCC TCAGCGACCA GGCCCAGCAC GTGCATGCCC TGGGGACCTT CCATCTCTTC
    TGCCAGGCCA CAGGTCCTGC GGATGTTCAC TTCCTCTGGG AGAAGAATGG GCGCGAGCTG
    GAGGTGTGTG TGCCCACGCA GACCCACGCA CTGCCCGATG GCCGGGCCCT TGTGCTCAGC
    TGGCTGCGAG ATGCGCTCCG GGAGAGCGCC GAGTACCGCT GCTCTGCGCT CTCCCGGGCC
    GGGAACAAGA CCTCCAAGGC GCGAGTCACC GTGACCAGGC TCGAGGCGAC CCAGCAGGAG
    AGGTGGACCA GAGAGCTCGC CAGCTGGAGG GCCGTGGTTG GGGAGCACGA CCGCATGATG
    CAGAGCTGGA GGAAGGCGTG GGAAAGCTGT AACCAAGACG CATTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001091629.1
    CDS399..2606
    Translation

    Target ORF information:

    RefSeq Version NM_001098160.1
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 4D (SEMA4D), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001098160.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCCGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGCCTCATC TCCTCTGCCC
    TTCCCTCCTC CGGGGTCCTC TTCCCTGTCC TGTCTGGGCC CCGACTCATC TTCCTCTCCC
    CGAAGCCCCT GGCCGGCCTC GGGCCCAGGG CCTGACCGCC GCCTGCAGGT CACCTTGCTG
    CCGCCCTTCC TCAGCGACCA GGCCCAGCAC GTGCATGCCC TGGGGACCTT CCATCTCTTC
    TGCCAGGCCA CAGGTCCTGC GGATGTTCAC TTCCTCTGGG AGAAGAATGG GCGCGAGCTG
    GAGGTGTGTG TGCCCACGCA GACCCACGCA CTGCCCGATG GCCGGGCCCT TGTGCTCAGC
    TGGCTGCGAG ATGCGCTCCG GGAGAGCGCC GAGTACCGCT GCTCTGCGCT CTCCCGGGCC
    GGGAACAAGA CCTCCAAGGC GCGAGTCACC GTGACCAGGC TCGAGGCGAC CCAGCAGGAG
    AGGTGGACCA GAGAGCTCGC CAGCTGGAGG GCCGTGGTTG GGGAGCACGA CCGCATGATG
    CAGAGCTGGA GGAAGGCGTG GGAAAGCTGT AACCAAGACG CATTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22316
    Clone ID Related Accession (Same CDS sequence) XM_015472659.1 , XM_005210463.4 , XM_015472658.1 , XM_005210464.3 , XM_005210466.2 , XM_024996004.1 , XM_005210463.3 , XM_005210464.4 , XM_005210466.3
    Accession Version XM_015472659.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-4D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104135.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGATAAAAT TAAAGAAAGT
    TACCTGCAGC ATTTTTTCAA GCATGGCGGC ACAGCAGAAC TCAAATGTTC CCAAAAGTCC
    AACCTGGCCC GGGTGGTATG GAAGTTCCAG AACGGCGTGC TCAAGGCCGA AAGCCCCAAG
    TACAGCTTGG TAGGCAGAAA GAACCTGCTC ATCTTCAACC TGTCGGAAGG AGACAGCGGG
    GTGTATCAGT GCCTGTCGGA GGAGAAGGTC AAGAACAAGA CGATCTTGCA GGTGGTGGCC
    AAGCATGTTC TGGAGGTCAA GACGGTCCAG CGAACCACAG TGGCCTCTAC CTCTCCAGCC
    ACACGGACAG AAGGCAGTCG GATCACCCCC AGAGTGTCAG TGGGGCCCAC CCCAGTCTCC
    TTTCCTCAGA CCCCAGCCGT GCAGGTCACC ACGCTGGGTG CTGTTCCCCC ACCATCCAGC
    CCCGTGGCAC CCACTAGTAC GTCCTGCGAA CCAAAGATCG TCATCAACAC GGTCCCGCAA
    GTCCACTCGG AGAAGACATT GTATCTAAAG TCCAGTGACA ACCGCCTCCT CATGTTTCTC
    TTCCTCTTCT TCTTCGCCCT CTTCCTCTGC CTCGTCTCCT ACAACTGCTA TAAGGGCTAC
    CTGCCCGGAC AGTGCTTGAA ATTCCGCTCG GCCATGCTCC TCGGGAAGAA GAAGCCCACG
    TCGGACTTCT CAGACTTTGA GCAGAGTGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC
    TCCCAGCAGA ATGGGGGACA GCCCAAGCCA GCCCTGGACA CCGGCTACGA GACAGAGCAG
    GACACGATGG CCAGCCGGCT GCCCACTGAC CGGGAGGACT CGCAGAAGAT CGACGAGCTC
    CCGGTCAGGG ACCGGCCGTT CGACGTCAAG TGCGAGCTGA AGTTCGCCGA CTCGGATGCG
    GACGGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328145.1
    CDS419..3010
    Translation

    Target ORF information:

    RefSeq Version XM_015472659.1
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 4D (SEMA4D), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472659.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGATAAAAT TAAAGAAAGT
    TACCTGCAGC ATTTTTTCAA GCATGGCGGC ACAGCAGAAC TCAAATGTTC CCAAAAGTCC
    AACCTGGCCC GGGTGGTATG GAAGTTCCAG AACGGCGTGC TCAAGGCCGA AAGCCCCAAG
    TACAGCTTGG TAGGCAGAAA GAACCTGCTC ATCTTCAACC TGTCGGAAGG AGACAGCGGG
    GTGTATCAGT GCCTGTCGGA GGAGAAGGTC AAGAACAAGA CGATCTTGCA GGTGGTGGCC
    AAGCATGTTC TGGAGGTCAA GACGGTCCAG CGAACCACAG TGGCCTCTAC CTCTCCAGCC
    ACACGGACAG AAGGCAGTCG GATCACCCCC AGAGTGTCAG TGGGGCCCAC CCCAGTCTCC
    TTTCCTCAGA CCCCAGCCGT GCAGGTCACC ACGCTGGGTG CTGTTCCCCC ACCATCCAGC
    CCCGTGGCAC CCACTAGTAC GTCCTGCGAA CCAAAGATCG TCATCAACAC GGTCCCGCAA
    GTCCACTCGG AGAAGACATT GTATCTAAAG TCCAGTGACA ACCGCCTCCT CATGTTTCTC
    TTCCTCTTCT TCTTCGCCCT CTTCCTCTGC CTCGTCTCCT ACAACTGCTA TAAGGGCTAC
    CTGCCCGGAC AGTGCTTGAA ATTCCGCTCG GCCATGCTCC TCGGGAAGAA GAAGCCCACG
    TCGGACTTCT CAGACTTTGA GCAGAGTGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC
    TCCCAGCAGA ATGGGGGACA GCCCAAGCCA GCCCTGGACA CCGGCTACGA GACAGAGCAG
    GACACGATGG CCAGCCGGCT GCCCACTGAC CGGGAGGACT CGCAGAAGAT CGACGAGCTC
    CCGGTCAGGG ACCGGCCGTT CGACGTCAAG TGCGAGCTGA AGTTCGCCGA CTCGGATGCG
    GACGGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22316
    Clone ID Related Accession (Same CDS sequence) XM_015472659.1 , XM_005210463.4 , XM_015472658.1 , XM_005210464.3 , XM_005210466.2 , XM_024996004.1 , XM_005210463.3 , XM_005210464.4 , XM_005210466.3
    Accession Version XM_005210463.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-4D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210463.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGATAAAAT TAAAGAAAGT
    TACCTGCAGC ATTTTTTCAA GCATGGCGGC ACAGCAGAAC TCAAATGTTC CCAAAAGTCC
    AACCTGGCCC GGGTGGTATG GAAGTTCCAG AACGGCGTGC TCAAGGCCGA AAGCCCCAAG
    TACAGCTTGG TAGGCAGAAA GAACCTGCTC ATCTTCAACC TGTCGGAAGG AGACAGCGGG
    GTGTATCAGT GCCTGTCGGA GGAGAAGGTC AAGAACAAGA CGATCTTGCA GGTGGTGGCC
    AAGCATGTTC TGGAGGTCAA GACGGTCCAG CGAACCACAG TGGCCTCTAC CTCTCCAGCC
    ACACGGACAG AAGGCAGTCG GATCACCCCC AGAGTGTCAG TGGGGCCCAC CCCAGTCTCC
    TTTCCTCAGA CCCCAGCCGT GCAGGTCACC ACGCTGGGTG CTGTTCCCCC ACCATCCAGC
    CCCGTGGCAC CCACTAGTAC GTCCTGCGAA CCAAAGATCG TCATCAACAC GGTCCCGCAA
    GTCCACTCGG AGAAGACATT GTATCTAAAG TCCAGTGACA ACCGCCTCCT CATGTTTCTC
    TTCCTCTTCT TCTTCGCCCT CTTCCTCTGC CTCGTCTCCT ACAACTGCTA TAAGGGCTAC
    CTGCCCGGAC AGTGCTTGAA ATTCCGCTCG GCCATGCTCC TCGGGAAGAA GAAGCCCACG
    TCGGACTTCT CAGACTTTGA GCAGAGTGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC
    TCCCAGCAGA ATGGGGGACA GCCCAAGCCA GCCCTGGACA CCGGCTACGA GACAGAGCAG
    GACACGATGG CCAGCCGGCT GCCCACTGAC CGGGAGGACT CGCAGAAGAT CGACGAGCTC
    CCGGTCAGGG ACCGGCCGTT CGACGTCAAG TGCGAGCTGA AGTTCGCCGA CTCGGATGCG
    GACGGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210520.1
    CDS430..3021
    Translation

    Target ORF information:

    RefSeq Version XM_005210463.4
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 4D (SEMA4D), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210463.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGATAAAAT TAAAGAAAGT
    TACCTGCAGC ATTTTTTCAA GCATGGCGGC ACAGCAGAAC TCAAATGTTC CCAAAAGTCC
    AACCTGGCCC GGGTGGTATG GAAGTTCCAG AACGGCGTGC TCAAGGCCGA AAGCCCCAAG
    TACAGCTTGG TAGGCAGAAA GAACCTGCTC ATCTTCAACC TGTCGGAAGG AGACAGCGGG
    GTGTATCAGT GCCTGTCGGA GGAGAAGGTC AAGAACAAGA CGATCTTGCA GGTGGTGGCC
    AAGCATGTTC TGGAGGTCAA GACGGTCCAG CGAACCACAG TGGCCTCTAC CTCTCCAGCC
    ACACGGACAG AAGGCAGTCG GATCACCCCC AGAGTGTCAG TGGGGCCCAC CCCAGTCTCC
    TTTCCTCAGA CCCCAGCCGT GCAGGTCACC ACGCTGGGTG CTGTTCCCCC ACCATCCAGC
    CCCGTGGCAC CCACTAGTAC GTCCTGCGAA CCAAAGATCG TCATCAACAC GGTCCCGCAA
    GTCCACTCGG AGAAGACATT GTATCTAAAG TCCAGTGACA ACCGCCTCCT CATGTTTCTC
    TTCCTCTTCT TCTTCGCCCT CTTCCTCTGC CTCGTCTCCT ACAACTGCTA TAAGGGCTAC
    CTGCCCGGAC AGTGCTTGAA ATTCCGCTCG GCCATGCTCC TCGGGAAGAA GAAGCCCACG
    TCGGACTTCT CAGACTTTGA GCAGAGTGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC
    TCCCAGCAGA ATGGGGGACA GCCCAAGCCA GCCCTGGACA CCGGCTACGA GACAGAGCAG
    GACACGATGG CCAGCCGGCT GCCCACTGAC CGGGAGGACT CGCAGAAGAT CGACGAGCTC
    CCGGTCAGGG ACCGGCCGTT CGACGTCAAG TGCGAGCTGA AGTTCGCCGA CTCGGATGCG
    GACGGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22316
    Clone ID Related Accession (Same CDS sequence) XM_015472659.1 , XM_005210463.4 , XM_015472658.1 , XM_005210464.3 , XM_005210466.2 , XM_024996004.1 , XM_005210463.3 , XM_005210464.4 , XM_005210466.3
    Accession Version XM_015472658.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-4D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104135.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGATAAAAT TAAAGAAAGT
    TACCTGCAGC ATTTTTTCAA GCATGGCGGC ACAGCAGAAC TCAAATGTTC CCAAAAGTCC
    AACCTGGCCC GGGTGGTATG GAAGTTCCAG AACGGCGTGC TCAAGGCCGA AAGCCCCAAG
    TACAGCTTGG TAGGCAGAAA GAACCTGCTC ATCTTCAACC TGTCGGAAGG AGACAGCGGG
    GTGTATCAGT GCCTGTCGGA GGAGAAGGTC AAGAACAAGA CGATCTTGCA GGTGGTGGCC
    AAGCATGTTC TGGAGGTCAA GACGGTCCAG CGAACCACAG TGGCCTCTAC CTCTCCAGCC
    ACACGGACAG AAGGCAGTCG GATCACCCCC AGAGTGTCAG TGGGGCCCAC CCCAGTCTCC
    TTTCCTCAGA CCCCAGCCGT GCAGGTCACC ACGCTGGGTG CTGTTCCCCC ACCATCCAGC
    CCCGTGGCAC CCACTAGTAC GTCCTGCGAA CCAAAGATCG TCATCAACAC GGTCCCGCAA
    GTCCACTCGG AGAAGACATT GTATCTAAAG TCCAGTGACA ACCGCCTCCT CATGTTTCTC
    TTCCTCTTCT TCTTCGCCCT CTTCCTCTGC CTCGTCTCCT ACAACTGCTA TAAGGGCTAC
    CTGCCCGGAC AGTGCTTGAA ATTCCGCTCG GCCATGCTCC TCGGGAAGAA GAAGCCCACG
    TCGGACTTCT CAGACTTTGA GCAGAGTGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC
    TCCCAGCAGA ATGGGGGACA GCCCAAGCCA GCCCTGGACA CCGGCTACGA GACAGAGCAG
    GACACGATGG CCAGCCGGCT GCCCACTGAC CGGGAGGACT CGCAGAAGAT CGACGAGCTC
    CCGGTCAGGG ACCGGCCGTT CGACGTCAAG TGCGAGCTGA AGTTCGCCGA CTCGGATGCG
    GACGGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328144.1
    CDS1285..3876
    Translation

    Target ORF information:

    RefSeq Version XM_015472658.1
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 4D (SEMA4D), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472658.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGATAAAAT TAAAGAAAGT
    TACCTGCAGC ATTTTTTCAA GCATGGCGGC ACAGCAGAAC TCAAATGTTC CCAAAAGTCC
    AACCTGGCCC GGGTGGTATG GAAGTTCCAG AACGGCGTGC TCAAGGCCGA AAGCCCCAAG
    TACAGCTTGG TAGGCAGAAA GAACCTGCTC ATCTTCAACC TGTCGGAAGG AGACAGCGGG
    GTGTATCAGT GCCTGTCGGA GGAGAAGGTC AAGAACAAGA CGATCTTGCA GGTGGTGGCC
    AAGCATGTTC TGGAGGTCAA GACGGTCCAG CGAACCACAG TGGCCTCTAC CTCTCCAGCC
    ACACGGACAG AAGGCAGTCG GATCACCCCC AGAGTGTCAG TGGGGCCCAC CCCAGTCTCC
    TTTCCTCAGA CCCCAGCCGT GCAGGTCACC ACGCTGGGTG CTGTTCCCCC ACCATCCAGC
    CCCGTGGCAC CCACTAGTAC GTCCTGCGAA CCAAAGATCG TCATCAACAC GGTCCCGCAA
    GTCCACTCGG AGAAGACATT GTATCTAAAG TCCAGTGACA ACCGCCTCCT CATGTTTCTC
    TTCCTCTTCT TCTTCGCCCT CTTCCTCTGC CTCGTCTCCT ACAACTGCTA TAAGGGCTAC
    CTGCCCGGAC AGTGCTTGAA ATTCCGCTCG GCCATGCTCC TCGGGAAGAA GAAGCCCACG
    TCGGACTTCT CAGACTTTGA GCAGAGTGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC
    TCCCAGCAGA ATGGGGGACA GCCCAAGCCA GCCCTGGACA CCGGCTACGA GACAGAGCAG
    GACACGATGG CCAGCCGGCT GCCCACTGAC CGGGAGGACT CGCAGAAGAT CGACGAGCTC
    CCGGTCAGGG ACCGGCCGTT CGACGTCAAG TGCGAGCTGA AGTTCGCCGA CTCGGATGCG
    GACGGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22316
    Clone ID Related Accession (Same CDS sequence) XM_015472659.1 , XM_005210463.4 , XM_015472658.1 , XM_005210464.3 , XM_005210466.2 , XM_024996004.1 , XM_005210463.3 , XM_005210464.4 , XM_005210466.3
    Accession Version XM_005210464.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-4D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104135.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210464.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGATAAAAT TAAAGAAAGT
    TACCTGCAGC ATTTTTTCAA GCATGGCGGC ACAGCAGAAC TCAAATGTTC CCAAAAGTCC
    AACCTGGCCC GGGTGGTATG GAAGTTCCAG AACGGCGTGC TCAAGGCCGA AAGCCCCAAG
    TACAGCTTGG TAGGCAGAAA GAACCTGCTC ATCTTCAACC TGTCGGAAGG AGACAGCGGG
    GTGTATCAGT GCCTGTCGGA GGAGAAGGTC AAGAACAAGA CGATCTTGCA GGTGGTGGCC
    AAGCATGTTC TGGAGGTCAA GACGGTCCAG CGAACCACAG TGGCCTCTAC CTCTCCAGCC
    ACACGGACAG AAGGCAGTCG GATCACCCCC AGAGTGTCAG TGGGGCCCAC CCCAGTCTCC
    TTTCCTCAGA CCCCAGCCGT GCAGGTCACC ACGCTGGGTG CTGTTCCCCC ACCATCCAGC
    CCCGTGGCAC CCACTAGTAC GTCCTGCGAA CCAAAGATCG TCATCAACAC GGTCCCGCAA
    GTCCACTCGG AGAAGACATT GTATCTAAAG TCCAGTGACA ACCGCCTCCT CATGTTTCTC
    TTCCTCTTCT TCTTCGCCCT CTTCCTCTGC CTCGTCTCCT ACAACTGCTA TAAGGGCTAC
    CTGCCCGGAC AGTGCTTGAA ATTCCGCTCG GCCATGCTCC TCGGGAAGAA GAAGCCCACG
    TCGGACTTCT CAGACTTTGA GCAGAGTGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC
    TCCCAGCAGA ATGGGGGACA GCCCAAGCCA GCCCTGGACA CCGGCTACGA GACAGAGCAG
    GACACGATGG CCAGCCGGCT GCCCACTGAC CGGGAGGACT CGCAGAAGAT CGACGAGCTC
    CCGGTCAGGG ACCGGCCGTT CGACGTCAAG TGCGAGCTGA AGTTCGCCGA CTCGGATGCG
    GACGGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210521.1
    CDS450..3041
    Translation

    Target ORF information:

    RefSeq Version XM_005210464.3
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 4D (SEMA4D), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210464.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGATAAAAT TAAAGAAAGT
    TACCTGCAGC ATTTTTTCAA GCATGGCGGC ACAGCAGAAC TCAAATGTTC CCAAAAGTCC
    AACCTGGCCC GGGTGGTATG GAAGTTCCAG AACGGCGTGC TCAAGGCCGA AAGCCCCAAG
    TACAGCTTGG TAGGCAGAAA GAACCTGCTC ATCTTCAACC TGTCGGAAGG AGACAGCGGG
    GTGTATCAGT GCCTGTCGGA GGAGAAGGTC AAGAACAAGA CGATCTTGCA GGTGGTGGCC
    AAGCATGTTC TGGAGGTCAA GACGGTCCAG CGAACCACAG TGGCCTCTAC CTCTCCAGCC
    ACACGGACAG AAGGCAGTCG GATCACCCCC AGAGTGTCAG TGGGGCCCAC CCCAGTCTCC
    TTTCCTCAGA CCCCAGCCGT GCAGGTCACC ACGCTGGGTG CTGTTCCCCC ACCATCCAGC
    CCCGTGGCAC CCACTAGTAC GTCCTGCGAA CCAAAGATCG TCATCAACAC GGTCCCGCAA
    GTCCACTCGG AGAAGACATT GTATCTAAAG TCCAGTGACA ACCGCCTCCT CATGTTTCTC
    TTCCTCTTCT TCTTCGCCCT CTTCCTCTGC CTCGTCTCCT ACAACTGCTA TAAGGGCTAC
    CTGCCCGGAC AGTGCTTGAA ATTCCGCTCG GCCATGCTCC TCGGGAAGAA GAAGCCCACG
    TCGGACTTCT CAGACTTTGA GCAGAGTGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC
    TCCCAGCAGA ATGGGGGACA GCCCAAGCCA GCCCTGGACA CCGGCTACGA GACAGAGCAG
    GACACGATGG CCAGCCGGCT GCCCACTGAC CGGGAGGACT CGCAGAAGAT CGACGAGCTC
    CCGGTCAGGG ACCGGCCGTT CGACGTCAAG TGCGAGCTGA AGTTCGCCGA CTCGGATGCG
    GACGGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22316
    Clone ID Related Accession (Same CDS sequence) XM_015472659.1 , XM_005210463.4 , XM_015472658.1 , XM_005210464.3 , XM_005210466.2 , XM_024996004.1 , XM_005210463.3 , XM_005210464.4 , XM_005210466.3
    Accession Version XM_005210466.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-4D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104135.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005210466.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGATAAAAT TAAAGAAAGT
    TACCTGCAGC ATTTTTTCAA GCATGGCGGC ACAGCAGAAC TCAAATGTTC CCAAAAGTCC
    AACCTGGCCC GGGTGGTATG GAAGTTCCAG AACGGCGTGC TCAAGGCCGA AAGCCCCAAG
    TACAGCTTGG TAGGCAGAAA GAACCTGCTC ATCTTCAACC TGTCGGAAGG AGACAGCGGG
    GTGTATCAGT GCCTGTCGGA GGAGAAGGTC AAGAACAAGA CGATCTTGCA GGTGGTGGCC
    AAGCATGTTC TGGAGGTCAA GACGGTCCAG CGAACCACAG TGGCCTCTAC CTCTCCAGCC
    ACACGGACAG AAGGCAGTCG GATCACCCCC AGAGTGTCAG TGGGGCCCAC CCCAGTCTCC
    TTTCCTCAGA CCCCAGCCGT GCAGGTCACC ACGCTGGGTG CTGTTCCCCC ACCATCCAGC
    CCCGTGGCAC CCACTAGTAC GTCCTGCGAA CCAAAGATCG TCATCAACAC GGTCCCGCAA
    GTCCACTCGG AGAAGACATT GTATCTAAAG TCCAGTGACA ACCGCCTCCT CATGTTTCTC
    TTCCTCTTCT TCTTCGCCCT CTTCCTCTGC CTCGTCTCCT ACAACTGCTA TAAGGGCTAC
    CTGCCCGGAC AGTGCTTGAA ATTCCGCTCG GCCATGCTCC TCGGGAAGAA GAAGCCCACG
    TCGGACTTCT CAGACTTTGA GCAGAGTGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC
    TCCCAGCAGA ATGGGGGACA GCCCAAGCCA GCCCTGGACA CCGGCTACGA GACAGAGCAG
    GACACGATGG CCAGCCGGCT GCCCACTGAC CGGGAGGACT CGCAGAAGAT CGACGAGCTC
    CCGGTCAGGG ACCGGCCGTT CGACGTCAAG TGCGAGCTGA AGTTCGCCGA CTCGGATGCG
    GACGGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210523.1
    CDS251..2842
    Translation

    Target ORF information:

    RefSeq Version XM_005210466.2
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 4D (SEMA4D), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210466.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGATAAAAT TAAAGAAAGT
    TACCTGCAGC ATTTTTTCAA GCATGGCGGC ACAGCAGAAC TCAAATGTTC CCAAAAGTCC
    AACCTGGCCC GGGTGGTATG GAAGTTCCAG AACGGCGTGC TCAAGGCCGA AAGCCCCAAG
    TACAGCTTGG TAGGCAGAAA GAACCTGCTC ATCTTCAACC TGTCGGAAGG AGACAGCGGG
    GTGTATCAGT GCCTGTCGGA GGAGAAGGTC AAGAACAAGA CGATCTTGCA GGTGGTGGCC
    AAGCATGTTC TGGAGGTCAA GACGGTCCAG CGAACCACAG TGGCCTCTAC CTCTCCAGCC
    ACACGGACAG AAGGCAGTCG GATCACCCCC AGAGTGTCAG TGGGGCCCAC CCCAGTCTCC
    TTTCCTCAGA CCCCAGCCGT GCAGGTCACC ACGCTGGGTG CTGTTCCCCC ACCATCCAGC
    CCCGTGGCAC CCACTAGTAC GTCCTGCGAA CCAAAGATCG TCATCAACAC GGTCCCGCAA
    GTCCACTCGG AGAAGACATT GTATCTAAAG TCCAGTGACA ACCGCCTCCT CATGTTTCTC
    TTCCTCTTCT TCTTCGCCCT CTTCCTCTGC CTCGTCTCCT ACAACTGCTA TAAGGGCTAC
    CTGCCCGGAC AGTGCTTGAA ATTCCGCTCG GCCATGCTCC TCGGGAAGAA GAAGCCCACG
    TCGGACTTCT CAGACTTTGA GCAGAGTGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC
    TCCCAGCAGA ATGGGGGACA GCCCAAGCCA GCCCTGGACA CCGGCTACGA GACAGAGCAG
    GACACGATGG CCAGCCGGCT GCCCACTGAC CGGGAGGACT CGCAGAAGAT CGACGAGCTC
    CCGGTCAGGG ACCGGCCGTT CGACGTCAAG TGCGAGCTGA AGTTCGCCGA CTCGGATGCG
    GACGGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22316
    Clone ID Related Accession (Same CDS sequence) XM_015472659.1 , XM_005210463.4 , XM_015472658.1 , XM_005210464.3 , XM_005210466.2 , XM_024996004.1 , XM_005210463.3 , XM_005210464.4 , XM_005210466.3
    Accession Version XM_024996004.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-4D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGATAAAAT TAAAGAAAGT
    TACCTGCAGC ATTTTTTCAA GCATGGCGGC ACAGCAGAAC TCAAATGTTC CCAAAAGTCC
    AACCTGGCCC GGGTGGTATG GAAGTTCCAG AACGGCGTGC TCAAGGCCGA AAGCCCCAAG
    TACAGCTTGG TAGGCAGAAA GAACCTGCTC ATCTTCAACC TGTCGGAAGG AGACAGCGGG
    GTGTATCAGT GCCTGTCGGA GGAGAAGGTC AAGAACAAGA CGATCTTGCA GGTGGTGGCC
    AAGCATGTTC TGGAGGTCAA GACGGTCCAG CGAACCACAG TGGCCTCTAC CTCTCCAGCC
    ACACGGACAG AAGGCAGTCG GATCACCCCC AGAGTGTCAG TGGGGCCCAC CCCAGTCTCC
    TTTCCTCAGA CCCCAGCCGT GCAGGTCACC ACGCTGGGTG CTGTTCCCCC ACCATCCAGC
    CCCGTGGCAC CCACTAGTAC GTCCTGCGAA CCAAAGATCG TCATCAACAC GGTCCCGCAA
    GTCCACTCGG AGAAGACATT GTATCTAAAG TCCAGTGACA ACCGCCTCCT CATGTTTCTC
    TTCCTCTTCT TCTTCGCCCT CTTCCTCTGC CTCGTCTCCT ACAACTGCTA TAAGGGCTAC
    CTGCCCGGAC AGTGCTTGAA ATTCCGCTCG GCCATGCTCC TCGGGAAGAA GAAGCCCACG
    TCGGACTTCT CAGACTTTGA GCAGAGTGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC
    TCCCAGCAGA ATGGGGGACA GCCCAAGCCA GCCCTGGACA CCGGCTACGA GACAGAGCAG
    GACACGATGG CCAGCCGGCT GCCCACTGAC CGGGAGGACT CGCAGAAGAT CGACGAGCTC
    CCGGTCAGGG ACCGGCCGTT CGACGTCAAG TGCGAGCTGA AGTTCGCCGA CTCGGATGCG
    GACGGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851772.1
    CDS323..2914
    Translation

    Target ORF information:

    RefSeq Version XM_024996004.1
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 4D (SEMA4D), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996004.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGATAAAAT TAAAGAAAGT
    TACCTGCAGC ATTTTTTCAA GCATGGCGGC ACAGCAGAAC TCAAATGTTC CCAAAAGTCC
    AACCTGGCCC GGGTGGTATG GAAGTTCCAG AACGGCGTGC TCAAGGCCGA AAGCCCCAAG
    TACAGCTTGG TAGGCAGAAA GAACCTGCTC ATCTTCAACC TGTCGGAAGG AGACAGCGGG
    GTGTATCAGT GCCTGTCGGA GGAGAAGGTC AAGAACAAGA CGATCTTGCA GGTGGTGGCC
    AAGCATGTTC TGGAGGTCAA GACGGTCCAG CGAACCACAG TGGCCTCTAC CTCTCCAGCC
    ACACGGACAG AAGGCAGTCG GATCACCCCC AGAGTGTCAG TGGGGCCCAC CCCAGTCTCC
    TTTCCTCAGA CCCCAGCCGT GCAGGTCACC ACGCTGGGTG CTGTTCCCCC ACCATCCAGC
    CCCGTGGCAC CCACTAGTAC GTCCTGCGAA CCAAAGATCG TCATCAACAC GGTCCCGCAA
    GTCCACTCGG AGAAGACATT GTATCTAAAG TCCAGTGACA ACCGCCTCCT CATGTTTCTC
    TTCCTCTTCT TCTTCGCCCT CTTCCTCTGC CTCGTCTCCT ACAACTGCTA TAAGGGCTAC
    CTGCCCGGAC AGTGCTTGAA ATTCCGCTCG GCCATGCTCC TCGGGAAGAA GAAGCCCACG
    TCGGACTTCT CAGACTTTGA GCAGAGTGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC
    TCCCAGCAGA ATGGGGGACA GCCCAAGCCA GCCCTGGACA CCGGCTACGA GACAGAGCAG
    GACACGATGG CCAGCCGGCT GCCCACTGAC CGGGAGGACT CGCAGAAGAT CGACGAGCTC
    CCGGTCAGGG ACCGGCCGTT CGACGTCAAG TGCGAGCTGA AGTTCGCCGA CTCGGATGCG
    GACGGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22316
    Clone ID Related Accession (Same CDS sequence) XM_015472659.1 , XM_005210463.4 , XM_015472658.1 , XM_005210464.3 , XM_005210466.2 , XM_024996004.1 , XM_005210463.3 , XM_005210464.4 , XM_005210466.3
    Accession Version XM_005210463.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product semaphorin-4D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104135.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210463.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGATAAAAT TAAAGAAAGT
    TACCTGCAGC ATTTTTTCAA GCATGGCGGC ACAGCAGAAC TCAAATGTTC CCAAAAGTCC
    AACCTGGCCC GGGTGGTATG GAAGTTCCAG AACGGCGTGC TCAAGGCCGA AAGCCCCAAG
    TACAGCTTGG TAGGCAGAAA GAACCTGCTC ATCTTCAACC TGTCGGAAGG AGACAGCGGG
    GTGTATCAGT GCCTGTCGGA GGAGAAGGTC AAGAACAAGA CGATCTTGCA GGTGGTGGCC
    AAGCATGTTC TGGAGGTCAA GACGGTCCAG CGAACCACAG TGGCCTCTAC CTCTCCAGCC
    ACACGGACAG AAGGCAGTCG GATCACCCCC AGAGTGTCAG TGGGGCCCAC CCCAGTCTCC
    TTTCCTCAGA CCCCAGCCGT GCAGGTCACC ACGCTGGGTG CTGTTCCCCC ACCATCCAGC
    CCCGTGGCAC CCACTAGTAC GTCCTGCGAA CCAAAGATCG TCATCAACAC GGTCCCGCAA
    GTCCACTCGG AGAAGACATT GTATCTAAAG TCCAGTGACA ACCGCCTCCT CATGTTTCTC
    TTCCTCTTCT TCTTCGCCCT CTTCCTCTGC CTCGTCTCCT ACAACTGCTA TAAGGGCTAC
    CTGCCCGGAC AGTGCTTGAA ATTCCGCTCG GCCATGCTCC TCGGGAAGAA GAAGCCCACG
    TCGGACTTCT CAGACTTTGA GCAGAGTGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC
    TCCCAGCAGA ATGGGGGACA GCCCAAGCCA GCCCTGGACA CCGGCTACGA GACAGAGCAG
    GACACGATGG CCAGCCGGCT GCCCACTGAC CGGGAGGACT CGCAGAAGAT CGACGAGCTC
    CCGGTCAGGG ACCGGCCGTT CGACGTCAAG TGCGAGCTGA AGTTCGCCGA CTCGGATGCG
    GACGGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210520.1
    CDS394..2985
    Translation

    Target ORF information:

    RefSeq Version XM_005210463.3
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 4D (SEMA4D), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210463.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGATAAAAT TAAAGAAAGT
    TACCTGCAGC ATTTTTTCAA GCATGGCGGC ACAGCAGAAC TCAAATGTTC CCAAAAGTCC
    AACCTGGCCC GGGTGGTATG GAAGTTCCAG AACGGCGTGC TCAAGGCCGA AAGCCCCAAG
    TACAGCTTGG TAGGCAGAAA GAACCTGCTC ATCTTCAACC TGTCGGAAGG AGACAGCGGG
    GTGTATCAGT GCCTGTCGGA GGAGAAGGTC AAGAACAAGA CGATCTTGCA GGTGGTGGCC
    AAGCATGTTC TGGAGGTCAA GACGGTCCAG CGAACCACAG TGGCCTCTAC CTCTCCAGCC
    ACACGGACAG AAGGCAGTCG GATCACCCCC AGAGTGTCAG TGGGGCCCAC CCCAGTCTCC
    TTTCCTCAGA CCCCAGCCGT GCAGGTCACC ACGCTGGGTG CTGTTCCCCC ACCATCCAGC
    CCCGTGGCAC CCACTAGTAC GTCCTGCGAA CCAAAGATCG TCATCAACAC GGTCCCGCAA
    GTCCACTCGG AGAAGACATT GTATCTAAAG TCCAGTGACA ACCGCCTCCT CATGTTTCTC
    TTCCTCTTCT TCTTCGCCCT CTTCCTCTGC CTCGTCTCCT ACAACTGCTA TAAGGGCTAC
    CTGCCCGGAC AGTGCTTGAA ATTCCGCTCG GCCATGCTCC TCGGGAAGAA GAAGCCCACG
    TCGGACTTCT CAGACTTTGA GCAGAGTGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC
    TCCCAGCAGA ATGGGGGACA GCCCAAGCCA GCCCTGGACA CCGGCTACGA GACAGAGCAG
    GACACGATGG CCAGCCGGCT GCCCACTGAC CGGGAGGACT CGCAGAAGAT CGACGAGCTC
    CCGGTCAGGG ACCGGCCGTT CGACGTCAAG TGCGAGCTGA AGTTCGCCGA CTCGGATGCG
    GACGGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22316
    Clone ID Related Accession (Same CDS sequence) XM_015472659.1 , XM_005210463.4 , XM_015472658.1 , XM_005210464.3 , XM_005210466.2 , XM_024996004.1 , XM_005210463.3 , XM_005210464.4 , XM_005210466.3
    Accession Version XM_005210464.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-4D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210464.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGATAAAAT TAAAGAAAGT
    TACCTGCAGC ATTTTTTCAA GCATGGCGGC ACAGCAGAAC TCAAATGTTC CCAAAAGTCC
    AACCTGGCCC GGGTGGTATG GAAGTTCCAG AACGGCGTGC TCAAGGCCGA AAGCCCCAAG
    TACAGCTTGG TAGGCAGAAA GAACCTGCTC ATCTTCAACC TGTCGGAAGG AGACAGCGGG
    GTGTATCAGT GCCTGTCGGA GGAGAAGGTC AAGAACAAGA CGATCTTGCA GGTGGTGGCC
    AAGCATGTTC TGGAGGTCAA GACGGTCCAG CGAACCACAG TGGCCTCTAC CTCTCCAGCC
    ACACGGACAG AAGGCAGTCG GATCACCCCC AGAGTGTCAG TGGGGCCCAC CCCAGTCTCC
    TTTCCTCAGA CCCCAGCCGT GCAGGTCACC ACGCTGGGTG CTGTTCCCCC ACCATCCAGC
    CCCGTGGCAC CCACTAGTAC GTCCTGCGAA CCAAAGATCG TCATCAACAC GGTCCCGCAA
    GTCCACTCGG AGAAGACATT GTATCTAAAG TCCAGTGACA ACCGCCTCCT CATGTTTCTC
    TTCCTCTTCT TCTTCGCCCT CTTCCTCTGC CTCGTCTCCT ACAACTGCTA TAAGGGCTAC
    CTGCCCGGAC AGTGCTTGAA ATTCCGCTCG GCCATGCTCC TCGGGAAGAA GAAGCCCACG
    TCGGACTTCT CAGACTTTGA GCAGAGTGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC
    TCCCAGCAGA ATGGGGGACA GCCCAAGCCA GCCCTGGACA CCGGCTACGA GACAGAGCAG
    GACACGATGG CCAGCCGGCT GCCCACTGAC CGGGAGGACT CGCAGAAGAT CGACGAGCTC
    CCGGTCAGGG ACCGGCCGTT CGACGTCAAG TGCGAGCTGA AGTTCGCCGA CTCGGATGCG
    GACGGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210521.1
    CDS494..3085
    Translation

    Target ORF information:

    RefSeq Version XM_005210464.4
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 4D (SEMA4D), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210464.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGATAAAAT TAAAGAAAGT
    TACCTGCAGC ATTTTTTCAA GCATGGCGGC ACAGCAGAAC TCAAATGTTC CCAAAAGTCC
    AACCTGGCCC GGGTGGTATG GAAGTTCCAG AACGGCGTGC TCAAGGCCGA AAGCCCCAAG
    TACAGCTTGG TAGGCAGAAA GAACCTGCTC ATCTTCAACC TGTCGGAAGG AGACAGCGGG
    GTGTATCAGT GCCTGTCGGA GGAGAAGGTC AAGAACAAGA CGATCTTGCA GGTGGTGGCC
    AAGCATGTTC TGGAGGTCAA GACGGTCCAG CGAACCACAG TGGCCTCTAC CTCTCCAGCC
    ACACGGACAG AAGGCAGTCG GATCACCCCC AGAGTGTCAG TGGGGCCCAC CCCAGTCTCC
    TTTCCTCAGA CCCCAGCCGT GCAGGTCACC ACGCTGGGTG CTGTTCCCCC ACCATCCAGC
    CCCGTGGCAC CCACTAGTAC GTCCTGCGAA CCAAAGATCG TCATCAACAC GGTCCCGCAA
    GTCCACTCGG AGAAGACATT GTATCTAAAG TCCAGTGACA ACCGCCTCCT CATGTTTCTC
    TTCCTCTTCT TCTTCGCCCT CTTCCTCTGC CTCGTCTCCT ACAACTGCTA TAAGGGCTAC
    CTGCCCGGAC AGTGCTTGAA ATTCCGCTCG GCCATGCTCC TCGGGAAGAA GAAGCCCACG
    TCGGACTTCT CAGACTTTGA GCAGAGTGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC
    TCCCAGCAGA ATGGGGGACA GCCCAAGCCA GCCCTGGACA CCGGCTACGA GACAGAGCAG
    GACACGATGG CCAGCCGGCT GCCCACTGAC CGGGAGGACT CGCAGAAGAT CGACGAGCTC
    CCGGTCAGGG ACCGGCCGTT CGACGTCAAG TGCGAGCTGA AGTTCGCCGA CTCGGATGCG
    GACGGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22316
    Clone ID Related Accession (Same CDS sequence) XM_015472659.1 , XM_005210463.4 , XM_015472658.1 , XM_005210464.3 , XM_005210466.2 , XM_024996004.1 , XM_005210463.3 , XM_005210464.4 , XM_005210466.3
    Accession Version XM_005210466.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-4D isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210466.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGATAAAAT TAAAGAAAGT
    TACCTGCAGC ATTTTTTCAA GCATGGCGGC ACAGCAGAAC TCAAATGTTC CCAAAAGTCC
    AACCTGGCCC GGGTGGTATG GAAGTTCCAG AACGGCGTGC TCAAGGCCGA AAGCCCCAAG
    TACAGCTTGG TAGGCAGAAA GAACCTGCTC ATCTTCAACC TGTCGGAAGG AGACAGCGGG
    GTGTATCAGT GCCTGTCGGA GGAGAAGGTC AAGAACAAGA CGATCTTGCA GGTGGTGGCC
    AAGCATGTTC TGGAGGTCAA GACGGTCCAG CGAACCACAG TGGCCTCTAC CTCTCCAGCC
    ACACGGACAG AAGGCAGTCG GATCACCCCC AGAGTGTCAG TGGGGCCCAC CCCAGTCTCC
    TTTCCTCAGA CCCCAGCCGT GCAGGTCACC ACGCTGGGTG CTGTTCCCCC ACCATCCAGC
    CCCGTGGCAC CCACTAGTAC GTCCTGCGAA CCAAAGATCG TCATCAACAC GGTCCCGCAA
    GTCCACTCGG AGAAGACATT GTATCTAAAG TCCAGTGACA ACCGCCTCCT CATGTTTCTC
    TTCCTCTTCT TCTTCGCCCT CTTCCTCTGC CTCGTCTCCT ACAACTGCTA TAAGGGCTAC
    CTGCCCGGAC AGTGCTTGAA ATTCCGCTCG GCCATGCTCC TCGGGAAGAA GAAGCCCACG
    TCGGACTTCT CAGACTTTGA GCAGAGTGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC
    TCCCAGCAGA ATGGGGGACA GCCCAAGCCA GCCCTGGACA CCGGCTACGA GACAGAGCAG
    GACACGATGG CCAGCCGGCT GCCCACTGAC CGGGAGGACT CGCAGAAGAT CGACGAGCTC
    CCGGTCAGGG ACCGGCCGTT CGACGTCAAG TGCGAGCTGA AGTTCGCCGA CTCGGATGCG
    GACGGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210523.1
    CDS266..2857
    Translation

    Target ORF information:

    RefSeq Version XM_005210466.3
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 4D (SEMA4D), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210466.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGATAAAAT TAAAGAAAGT
    TACCTGCAGC ATTTTTTCAA GCATGGCGGC ACAGCAGAAC TCAAATGTTC CCAAAAGTCC
    AACCTGGCCC GGGTGGTATG GAAGTTCCAG AACGGCGTGC TCAAGGCCGA AAGCCCCAAG
    TACAGCTTGG TAGGCAGAAA GAACCTGCTC ATCTTCAACC TGTCGGAAGG AGACAGCGGG
    GTGTATCAGT GCCTGTCGGA GGAGAAGGTC AAGAACAAGA CGATCTTGCA GGTGGTGGCC
    AAGCATGTTC TGGAGGTCAA GACGGTCCAG CGAACCACAG TGGCCTCTAC CTCTCCAGCC
    ACACGGACAG AAGGCAGTCG GATCACCCCC AGAGTGTCAG TGGGGCCCAC CCCAGTCTCC
    TTTCCTCAGA CCCCAGCCGT GCAGGTCACC ACGCTGGGTG CTGTTCCCCC ACCATCCAGC
    CCCGTGGCAC CCACTAGTAC GTCCTGCGAA CCAAAGATCG TCATCAACAC GGTCCCGCAA
    GTCCACTCGG AGAAGACATT GTATCTAAAG TCCAGTGACA ACCGCCTCCT CATGTTTCTC
    TTCCTCTTCT TCTTCGCCCT CTTCCTCTGC CTCGTCTCCT ACAACTGCTA TAAGGGCTAC
    CTGCCCGGAC AGTGCTTGAA ATTCCGCTCG GCCATGCTCC TCGGGAAGAA GAAGCCCACG
    TCGGACTTCT CAGACTTTGA GCAGAGTGTG AAGGAGACGC TGGTGGAGCA GGGCAGCTTC
    TCCCAGCAGA ATGGGGGACA GCCCAAGCCA GCCCTGGACA CCGGCTACGA GACAGAGCAG
    GACACGATGG CCAGCCGGCT GCCCACTGAC CGGGAGGACT CGCAGAAGAT CGACGAGCTC
    CCGGTCAGGG ACCGGCCGTT CGACGTCAAG TGCGAGCTGA AGTTCGCCGA CTCGGATGCG
    GACGGGGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237302
    Clone ID Related Accession (Same CDS sequence) XM_024996005.1 , XM_024996006.1
    Accession Version XM_024996005.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-4D isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGCCTCATC TCCTCTGCCC
    TTCCCTCCTC CGGGGTCCTC TTCCCTGTCC TGTCTGGGCC CCGACTCATC TTCCTCTCCC
    CGAAGCCCCT GGCCGGCCTC GGGCCCAGGG CCTGACCGCC GCCTGCAGGT CACCTTGCTG
    CCGCCCTTCC TCAGCGACCA GGCCCAGCAC GTGCATGCCC TGGGGACCTT CCATCTCTTC
    TGCCAGGCCA CAGGTCCTGC GGATGTTCAC TTCCTCTGGG AGAAGAATGG GCGCGAGCTG
    GAGGTGTGTG TGCCCACGCA GACCCACGCA CTGCCCGATG GCCGGGCCCT TGTGCTCAGC
    TGGCTGCGAG ATGCGCTCCG GGAGAGCGCC GAGTACCGCT GCTCTGCGCT CTCCCGGGCC
    GGGAACAAGA CCTCCAAGGC GCGCGTCACC GTGACCAGGC TCGAGGCGAC CCAGCAGGAG
    AGGTGGACCA GAGAGCTCGC CAGCTGGAGG GCCGTGGTTG GGGAGCACGA CCGCATGATG
    CAGAGCTGGA GGAAGGCGTG GGAAAGCTGT AACCAAGACG CATTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851773.1
    CDS392..2599
    Translation

    Target ORF information:

    RefSeq Version XM_024996005.1
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 4D (SEMA4D), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996005.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGCCTCATC TCCTCTGCCC
    TTCCCTCCTC CGGGGTCCTC TTCCCTGTCC TGTCTGGGCC CCGACTCATC TTCCTCTCCC
    CGAAGCCCCT GGCCGGCCTC GGGCCCAGGG CCTGACCGCC GCCTGCAGGT CACCTTGCTG
    CCGCCCTTCC TCAGCGACCA GGCCCAGCAC GTGCATGCCC TGGGGACCTT CCATCTCTTC
    TGCCAGGCCA CAGGTCCTGC GGATGTTCAC TTCCTCTGGG AGAAGAATGG GCGCGAGCTG
    GAGGTGTGTG TGCCCACGCA GACCCACGCA CTGCCCGATG GCCGGGCCCT TGTGCTCAGC
    TGGCTGCGAG ATGCGCTCCG GGAGAGCGCC GAGTACCGCT GCTCTGCGCT CTCCCGGGCC
    GGGAACAAGA CCTCCAAGGC GCGCGTCACC GTGACCAGGC TCGAGGCGAC CCAGCAGGAG
    AGGTGGACCA GAGAGCTCGC CAGCTGGAGG GCCGTGGTTG GGGAGCACGA CCGCATGATG
    CAGAGCTGGA GGAAGGCGTG GGAAAGCTGT AACCAAGACG CATTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237302
    Clone ID Related Accession (Same CDS sequence) XM_024996005.1 , XM_024996006.1
    Accession Version XM_024996006.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product semaphorin-4D isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGCCTCATC TCCTCTGCCC
    TTCCCTCCTC CGGGGTCCTC TTCCCTGTCC TGTCTGGGCC CCGACTCATC TTCCTCTCCC
    CGAAGCCCCT GGCCGGCCTC GGGCCCAGGG CCTGACCGCC GCCTGCAGGT CACCTTGCTG
    CCGCCCTTCC TCAGCGACCA GGCCCAGCAC GTGCATGCCC TGGGGACCTT CCATCTCTTC
    TGCCAGGCCA CAGGTCCTGC GGATGTTCAC TTCCTCTGGG AGAAGAATGG GCGCGAGCTG
    GAGGTGTGTG TGCCCACGCA GACCCACGCA CTGCCCGATG GCCGGGCCCT TGTGCTCAGC
    TGGCTGCGAG ATGCGCTCCG GGAGAGCGCC GAGTACCGCT GCTCTGCGCT CTCCCGGGCC
    GGGAACAAGA CCTCCAAGGC GCGCGTCACC GTGACCAGGC TCGAGGCGAC CCAGCAGGAG
    AGGTGGACCA GAGAGCTCGC CAGCTGGAGG GCCGTGGTTG GGGAGCACGA CCGCATGATG
    CAGAGCTGGA GGAAGGCGTG GGAAAGCTGT AACCAAGACG CATTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851774.1
    CDS373..2580
    Translation

    Target ORF information:

    RefSeq Version XM_024996006.1
    Organism Bos taurus(cattle)
    Definition Bos taurus semaphorin 4D (SEMA4D), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996006.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGGATGT GTGGCCCTGT TGGGGGGCTG CTCACCGCCC TCGCCGTGGT GTTTGGGACG 
    GCTGTGGCAT TTGCTCCCGT ACCTAGGATC ACTTGGGAGC ACAGAGAGGT ACAGCTGGTG
    CAGTTTCACG AGCCGGGCAT CTTCAACTAC TCAGCCCTGC TGCTGAGTGA GAATGAGGAC
    ACCTTGTTTG TGGGTGCCCG GGAAGCTGTG TTTGCCTTAA ACGCTCACAA CATCTCCAAG
    AAGCAGCATG AGGCTTATTG GAAGGTTTCA GAAGATAAAA AAGCAAGATG TGCAGAAAAG
    GGGAAGTCAA AACAGACAGA ATGCCTCAAC TACATCCGTG TGTTGCAGCC ACTCAGCACC
    AACGCCCTTT ACGTGTGCGG GACCAATGCG TTCCAGCCAG CCTGTGACCA CCTGAACTTA
    ACATCCTTCA AATTTCTGGG GAAAAATGAA GATGGCAAAG GAAGGTGTCC CTTTGACCCA
    GCACAGAGCT ACACGTCTGT CATGGTCGAT GGAGAGCTTT ATTCCGGGAC GTCGTACAAC
    TTTCTGGGAA GTGAACCTAT CATCTCCCGA AATTCTACAC ACAGTCCTCT GAGGACAGAG
    TATGCAATTC CTTGGCTTAA TGAGCCCAGC TTCGTGTTTG CTGACGTGGT GCGAGAGAGC
    CCGGAGGGCG AGGACCTCGG GGACGACAGG GTGTACTTCT TCTTCACGGA GGTGTCTGTG
    GAGTACGAGT TTGTCTTCAA GCTGATGATC CCCCGGATAG CGAGGGTCTG CAAGGGGGAC
    CAGGGGGGCC TGAGGACCTT ACAGAAGAAG TGGACCTCCT TCCTGAAGGC CAGGCTCATC
    TGCTCCCGGC CGGACAGCAA CCTCGTCTTT AACGTGCTGC GGGATGTCTT CGTCCTCAGG
    TCTGCGGACC TGAAGGAACC CGTGTTCTAC GGGGTCTTCA CCCCGCAGCT GAACAACGTG
    GGGCTGTCGG CCGTGTGCGC CTACAACCTG TCCACGGCTG AGGCGGTCTT CTCCCGTGGG
    AAGTACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGG
    GCTGTGCCCA CGCCGCGGCC CGGGGCGTGT ATCAACCGGG AGGCACGGGC GGCCAACTTT
    TCTAGCTCGC TCAACCTGCC GGACAAGACC CTGCAGTTCG TCAAAGACCA CCCCCTGATG
    GACGACTCCG TGACCCCGAT AGACAGTAGG CCCCGGCTGA TCAAAGGCGA CGTGAACTAC
    ACGCAGATTG TGGTGGACAG GGCCCGGGCC CTGGATGGAA CCATCCACGA CGTCATGTTT
    GTCAGCACAG ACCGGGGAGC CCTGCACAAA GCCATCAGCC TTGAGAATGA CATCCACATC
    ATTGAGGAGA CTCAGCTCTT CCAGGACTTC GAGCCCATCC AGAGCCTGCT GCTGTCTTCC
    AAGAAGGGCA GGAAGTTTGT CTATGCCGGT TCCAACTCAG GCGTGGTGCA GGCCCCTGTG
    GCCTTCTGCG GGAAGCATGG CACCTGCGAG GACTGCGTGC TTGCCCGGGA CCCCTACTGC
    GCCTGGAGCC CGGCCGTGGC CGCCTGTGTC GCCCTGCACC AGACTGACAG CCCCAGCAGG
    GGACTGATTC AGGAAATGAA TGGGGACGCA TCCACTTGCC CTGCCTCATC TCCTCTGCCC
    TTCCCTCCTC CGGGGTCCTC TTCCCTGTCC TGTCTGGGCC CCGACTCATC TTCCTCTCCC
    CGAAGCCCCT GGCCGGCCTC GGGCCCAGGG CCTGACCGCC GCCTGCAGGT CACCTTGCTG
    CCGCCCTTCC TCAGCGACCA GGCCCAGCAC GTGCATGCCC TGGGGACCTT CCATCTCTTC
    TGCCAGGCCA CAGGTCCTGC GGATGTTCAC TTCCTCTGGG AGAAGAATGG GCGCGAGCTG
    GAGGTGTGTG TGCCCACGCA GACCCACGCA CTGCCCGATG GCCGGGCCCT TGTGCTCAGC
    TGGCTGCGAG ATGCGCTCCG GGAGAGCGCC GAGTACCGCT GCTCTGCGCT CTCCCGGGCC
    GGGAACAAGA CCTCCAAGGC GCGCGTCACC GTGACCAGGC TCGAGGCGAC CCAGCAGGAG
    AGGTGGACCA GAGAGCTCGC CAGCTGGAGG GCCGTGGTTG GGGAGCACGA CCGCATGATG
    CAGAGCTGGA GGAAGGCGTG GGAAAGCTGT AACCAAGACG CATTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.