ROCK1 cDNA ORF clone, Bos taurus(cattle)

The following ROCK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROCK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa230092 XM_002697789.6
Latest version!
Bos taurus Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa230093 XM_024984560.1
Latest version!
Bos taurus Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa88329 XM_002697789.5
Latest version!
Bos taurus Rho associated coiled-coil containing protein kinase 1 (ROCK1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa88329 XM_015469135.1
Latest version!
Bos taurus Rho associated coiled-coil containing protein kinase 1 (ROCK1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa230092
    Clone ID Related Accession (Same CDS sequence) XM_002697789.6
    Accession Version XM_002697789.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho-associated protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002697789.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GCGACAGTTT TGAGACTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCCAAATCGG AAGTGAATTC AGATTGTCTG CTGGATGGAT TGGATGCTTT GGTATATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGA ATGAAAGCTG AAGATTATGA AGTAGTGAAG
    GTGATTGGTA GAGGTGCATT TGGAGAAGTT CAATTGGTAA GGCATAAATC CACCAGGAAG
    GTGTACGCTA TGAAGCTCCT CAGCAAATTT GAAATGATTA AGAGATCTGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCTTTT GCTAACAGTC CTTGGGTTGT TCAGCTGTTT
    TATGCATTCC AAGATGATCG TTATCTTTAC ATGGTAATGG AGTACATGCC TGGTGGAGAT
    CTTGTAAATT TAATGAGCAA CTATGACGTG CCTGAAAAAT GGGCCCGATT CTATACTGCA
    GAAGTAGTTC TTGCATTGGA TGCAATCCAT TCCATGGGTT TTATCCATAG AGATGTGAAG
    CCTGATAACA TGCTGCTGGA TAAATCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG CATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGATTAT
    ATTTCCCCTG AAGTATTAAA ATCTCAAGGT GGCGATGGTT ATTATGGACG AGAATGTGAC
    TGGTGGTCAG TTGGGGTATT TTTATATGAA ATGCTTGTAG GTGATACACC ATTTTATGCA
    GATTCTCTGG TTGGAACTTA CAGTAAGATT ATGAACCATA AAAATTCTCT TACCTTCCCT
    GATGATAATG ACATATCAAA AGAAGCAAAA AATCTTATTT GTGCCTTCCT TACTGACAGA
    GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT
    GATCAATGGG CTTGGGAAAC ACTTCGAGAC ACTGTAGCAC CAGTTGTACC TGATTTAAGT
    AGTGACATAG ACACAAGTAA CTTTGATGAC TTGGAAGAAG ATAAAGGAGA TGAAGAAACA
    TTCCCTATAC CTAAAGCTTT TGTTGGCAAT CAACTACCTT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTATC TTCAGCAAAT CCTAACGATA ACAGAACTAG CTCTAATGTG
    GATAAAAGCT TGCAGGAAAA TTTGCAGAAA ACAATTTATA AGCTGGAAGA ACAGTTACAT
    AATGAAATGC AATTAAAAGA TGAAATGGAG CAGAAGTGCA GAACGTCAAA CATAAAACTA
    GACAAGATAA TGAAAGAATT GGACGAAGAG GGGAATCAAA GAAGAAATCT AGAATCTACA
    GTATCTCAGA TTGAGAAGGA GAAAATGTTG CTACAGCACA GAATTAATGA ATACCAAAGA
    AAAGCAGAAC AGGAAAATGA GAAGAGGAGG AATGTAGAAA ATGAAGTTTC TACATTAAAG
    GATCAGTTGG AAGACTTAAA GAAAGTCAGC CAGAATTCAC AGCTTGCTAA TGAGAAGCTG
    GCACAATTAC AAAAGCAGCT AGAAGAAGCC AATGACTTAC TTAGGACAGA ATCAGACACA
    GCGGTTAGAT TAAGGAAGAG TCACACAGAG ATGAGCAAAT CCATCACTCA GCTAGAGTCC
    CTAAACAGAG AACTGCAGGA GAGAAATAGA ATTTTAGAGA ATTCCAAGTC ACAAATGGAC
    AAAGATTATT ACCAACTACA GGCTGTATTA GAAGCTGAAC GAAGAGACAG AGGTCATGAT
    TCTGAGAAGA TTGGAGACCT ACAAGCTCGA ATTACATCTT TACAAGAGGA GGTGAAGCAT
    CTCAAACATA ATCTTGAAAG AGTGGAAGGA GAAAGAAAAG AGGCTCAAGA CATGCTTAAT
    CATTCAGAAA AGGAAAAGAA TAACTTAGAA ATAGATTTAA ACTACAAGCT TAAATCATTA
    CAGCAACGAT TAGAACAAGA GGTAAATGAA CATAAAGTAA CCAAAGCTCG TTTAACTGAC
    AAACATCAAT CTATTGAAGA GGCAAAGTCT GTTGCAATGT GTGAGATGGA AAAAAAGCTG
    AAGGAGGAGA GAGACGCTCG AGAGAAGGCT GAAAATAGGG TTGTTCAGAT TGAGAAGCAG
    TGCTCTATGC TAGATGTTGA TCTGAAGCAA TCTCAGCAGA AGCTGGAGCA TTTGATTGAA
    AATAAAGACA GGATGGAGGA TGAAGTTAAG AATCTAACGC TGCAGCTGGA GCAGGAATCA
    AACAAGCGAC TTTTGTTACA AAATGAATTG AAGACTCAAG CATTTGAGGC AGACAATTTA
    AAAGGTTTAG AAAAGCAGAT GAAACAGGAA ATAAATACTT TATTGGAAGC AAAGAGATTG
    TTGGAATTTG AATTAGCTCA GCTTACAAAA CAGTACAGAG GGAATGAAGG GCAGATGCGG
    GAGCTGCAGG ATCAGCTGGA AGCGGAGCAG TATTTCTCGA CACTTTATAA AACCCAGGTA
    AAGGAACTTA AGGAAGAAAT TGAAGAAAAA AACAGAGAAA ACTTAAAGAA AATACAGGAA
    CTACAAAGTG AAAAAGAAAC TCTTGCTACT CAGTTGGATC TAGCAGAAAC AAAGGCTGAA
    TCTGAGCAGT TGGCTCGAGG GCTTCTAGAA GAACAGTATT TTGAACTGAC CCAAGAAAGC
    AAGAAAGCTG CCTCAAGAAA TAGACAAGAG ATTACAGATA AAGATCACAC TCTTAGTCGG
    CTTGAAGAAA CGAATAGCAT GCTAACCAAA GATATTGAAT TATTAAGAAA AGAAAACGAA
    GAGCTAACTG ATAAAATGAG GAAGGCAGAG GAAGAATATA AATTGAAGAA GGAGGAGGAG
    ATCAATATCC TTAAGGCTGC CTTTGAAAAG AATATCAATA CAGAACGAAC CCTTAAAACA
    CAGGCTGTTA ACAAGTTGGC AGAAATAATG AATCGAAAGG ACTTTAAAAT TGATAGAAAG
    AAAGCAAATA CGCAGGATTT GAGAAAGAAA GAAAAAGAAA ATCGAAAGCT CCAACTTGAA
    CTCAACCAGG AAAGAGAGAA ATTCAACCAA ATGGTAGTGA AACATCAGAA GGAGCTGAAT
    GAAATGCAAG CGCAATTGGT AGAAGAATGT ACACATAAGA ATGAGCTTCA GATGCAGCTG
    GCCAGCAAGG AGAGTGACAT TGAGCAGCTG CGTGCCAAAC TGTTGGACCT TTCAGATTCT
    ACAAGTGTTG CTAGTTTTCC CAGTGCTGAT GAAACTGATG GAAACCTTCC AGAATCAAGA
    ATTGAAGGTT GGCTTTCAAT ACCAAATAGA GGAAATATCA AAAGATATGG CTGGAAGAAA
    CAGTATGTTG TGGTAAGCAG CAAAAAAATT TTGTTCTATA ATGATGAACA AGATAAGGAA
    CAATCCAATC CGTCTATGGT ATTGGACATT GATAAATTGT TTCATGTTCG TCCTGTAACA
    CAAGGAGATG TATACAGAGC TGAAACTGAA GAAATTCCTA AAATATTCCA GATACTGTAT
    GCAAATGAAG GTGAATGTAG AAAAGACTTA GAGGTGGAAC CAGTACAACA AGCTGAAAAA
    ACAAATTTTC AAAACCACAA AGGCCATGAG TTCATTCCTA CACTCTACCA CTTTCCCGCC
    AACTGTGAGG CCTGTGCCAA ACCTCTCTGG CATGTTTTTA AGCCACCCCC TGCCCTGGAG
    TGTCGAAGAT GCCATGTTAA GTGCCACAGA GACCACATAG ATAAGAAAGA GGACTTGATT
    TCTCCATGTA AAGTAAGTTA TGATGTAACA TCAGCAAGAG ATATGCTGCT GCTAGCGTGT
    TCTCAGGATG AACAAAAAAA ATGGGTAACT CATTTAGTAA AGAAGATCCC TAAGAATCCA
    CCATCTGGTT TTGTGCGTGC ATCCCCTCGA ACACTTTCTA CAAGGTCCAC TGCAAATCAG
    TCTTTCCGGA AAGTGGTCAA AAATACATCT GGAAAAGCTA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002697835.5
    CDS972..5036
    Translation

    Target ORF information:

    RefSeq Version XM_002697789.6
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002697789.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GCGACAGTTT TGAGACTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCCAAATCGG AAGTGAATTC AGATTGTCTG CTGGATGGAT TGGATGCTTT GGTATATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGA ATGAAAGCTG AAGATTATGA AGTAGTGAAG
    GTGATTGGTA GAGGTGCATT TGGAGAAGTT CAATTGGTAA GGCATAAATC CACCAGGAAG
    GTGTACGCTA TGAAGCTCCT CAGCAAATTT GAAATGATTA AGAGATCTGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCTTTT GCTAACAGTC CTTGGGTTGT TCAGCTGTTT
    TATGCATTCC AAGATGATCG TTATCTTTAC ATGGTAATGG AGTACATGCC TGGTGGAGAT
    CTTGTAAATT TAATGAGCAA CTATGACGTG CCTGAAAAAT GGGCCCGATT CTATACTGCA
    GAAGTAGTTC TTGCATTGGA TGCAATCCAT TCCATGGGTT TTATCCATAG AGATGTGAAG
    CCTGATAACA TGCTGCTGGA TAAATCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG CATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGATTAT
    ATTTCCCCTG AAGTATTAAA ATCTCAAGGT GGCGATGGTT ATTATGGACG AGAATGTGAC
    TGGTGGTCAG TTGGGGTATT TTTATATGAA ATGCTTGTAG GTGATACACC ATTTTATGCA
    GATTCTCTGG TTGGAACTTA CAGTAAGATT ATGAACCATA AAAATTCTCT TACCTTCCCT
    GATGATAATG ACATATCAAA AGAAGCAAAA AATCTTATTT GTGCCTTCCT TACTGACAGA
    GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT
    GATCAATGGG CTTGGGAAAC ACTTCGAGAC ACTGTAGCAC CAGTTGTACC TGATTTAAGT
    AGTGACATAG ACACAAGTAA CTTTGATGAC TTGGAAGAAG ATAAAGGAGA TGAAGAAACA
    TTCCCTATAC CTAAAGCTTT TGTTGGCAAT CAACTACCTT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTATC TTCAGCAAAT CCTAACGATA ACAGAACTAG CTCTAATGTG
    GATAAAAGCT TGCAGGAAAA TTTGCAGAAA ACAATTTATA AGCTGGAAGA ACAGTTACAT
    AATGAAATGC AATTAAAAGA TGAAATGGAG CAGAAGTGCA GAACGTCAAA CATAAAACTA
    GACAAGATAA TGAAAGAATT GGACGAAGAG GGGAATCAAA GAAGAAATCT AGAATCTACA
    GTATCTCAGA TTGAGAAGGA GAAAATGTTG CTACAGCACA GAATTAATGA ATACCAAAGA
    AAAGCAGAAC AGGAAAATGA GAAGAGGAGG AATGTAGAAA ATGAAGTTTC TACATTAAAG
    GATCAGTTGG AAGACTTAAA GAAAGTCAGC CAGAATTCAC AGCTTGCTAA TGAGAAGCTG
    GCACAATTAC AAAAGCAGCT AGAAGAAGCC AATGACTTAC TTAGGACAGA ATCAGACACA
    GCGGTTAGAT TAAGGAAGAG TCACACAGAG ATGAGCAAAT CCATCACTCA GCTAGAGTCC
    CTAAACAGAG AACTGCAGGA GAGAAATAGA ATTTTAGAGA ATTCCAAGTC ACAAATGGAC
    AAAGATTATT ACCAACTACA GGCTGTATTA GAAGCTGAAC GAAGAGACAG AGGTCATGAT
    TCTGAGAAGA TTGGAGACCT ACAAGCTCGA ATTACATCTT TACAAGAGGA GGTGAAGCAT
    CTCAAACATA ATCTTGAAAG AGTGGAAGGA GAAAGAAAAG AGGCTCAAGA CATGCTTAAT
    CATTCAGAAA AGGAAAAGAA TAACTTAGAA ATAGATTTAA ACTACAAGCT TAAATCATTA
    CAGCAACGAT TAGAACAAGA GGTAAATGAA CATAAAGTAA CCAAAGCTCG TTTAACTGAC
    AAACATCAAT CTATTGAAGA GGCAAAGTCT GTTGCAATGT GTGAGATGGA AAAAAAGCTG
    AAGGAGGAGA GAGACGCTCG AGAGAAGGCT GAAAATAGGG TTGTTCAGAT TGAGAAGCAG
    TGCTCTATGC TAGATGTTGA TCTGAAGCAA TCTCAGCAGA AGCTGGAGCA TTTGATTGAA
    AATAAAGACA GGATGGAGGA TGAAGTTAAG AATCTAACGC TGCAGCTGGA GCAGGAATCA
    AACAAGCGAC TTTTGTTACA AAATGAATTG AAGACTCAAG CATTTGAGGC AGACAATTTA
    AAAGGTTTAG AAAAGCAGAT GAAACAGGAA ATAAATACTT TATTGGAAGC AAAGAGATTG
    TTGGAATTTG AATTAGCTCA GCTTACAAAA CAGTACAGAG GGAATGAAGG GCAGATGCGG
    GAGCTGCAGG ATCAGCTGGA AGCGGAGCAG TATTTCTCGA CACTTTATAA AACCCAGGTA
    AAGGAACTTA AGGAAGAAAT TGAAGAAAAA AACAGAGAAA ACTTAAAGAA AATACAGGAA
    CTACAAAGTG AAAAAGAAAC TCTTGCTACT CAGTTGGATC TAGCAGAAAC AAAGGCTGAA
    TCTGAGCAGT TGGCTCGAGG GCTTCTAGAA GAACAGTATT TTGAACTGAC CCAAGAAAGC
    AAGAAAGCTG CCTCAAGAAA TAGACAAGAG ATTACAGATA AAGATCACAC TCTTAGTCGG
    CTTGAAGAAA CGAATAGCAT GCTAACCAAA GATATTGAAT TATTAAGAAA AGAAAACGAA
    GAGCTAACTG ATAAAATGAG GAAGGCAGAG GAAGAATATA AATTGAAGAA GGAGGAGGAG
    ATCAATATCC TTAAGGCTGC CTTTGAAAAG AATATCAATA CAGAACGAAC CCTTAAAACA
    CAGGCTGTTA ACAAGTTGGC AGAAATAATG AATCGAAAGG ACTTTAAAAT TGATAGAAAG
    AAAGCAAATA CGCAGGATTT GAGAAAGAAA GAAAAAGAAA ATCGAAAGCT CCAACTTGAA
    CTCAACCAGG AAAGAGAGAA ATTCAACCAA ATGGTAGTGA AACATCAGAA GGAGCTGAAT
    GAAATGCAAG CGCAATTGGT AGAAGAATGT ACACATAAGA ATGAGCTTCA GATGCAGCTG
    GCCAGCAAGG AGAGTGACAT TGAGCAGCTG CGTGCCAAAC TGTTGGACCT TTCAGATTCT
    ACAAGTGTTG CTAGTTTTCC CAGTGCTGAT GAAACTGATG GAAACCTTCC AGAATCAAGA
    ATTGAAGGTT GGCTTTCAAT ACCAAATAGA GGAAATATCA AAAGATATGG CTGGAAGAAA
    CAGTATGTTG TGGTAAGCAG CAAAAAAATT TTGTTCTATA ATGATGAACA AGATAAGGAA
    CAATCCAATC CGTCTATGGT ATTGGACATT GATAAATTGT TTCATGTTCG TCCTGTAACA
    CAAGGAGATG TATACAGAGC TGAAACTGAA GAAATTCCTA AAATATTCCA GATACTGTAT
    GCAAATGAAG GTGAATGTAG AAAAGACTTA GAGGTGGAAC CAGTACAACA AGCTGAAAAA
    ACAAATTTTC AAAACCACAA AGGCCATGAG TTCATTCCTA CACTCTACCA CTTTCCCGCC
    AACTGTGAGG CCTGTGCCAA ACCTCTCTGG CATGTTTTTA AGCCACCCCC TGCCCTGGAG
    TGTCGAAGAT GCCATGTTAA GTGCCACAGA GACCACATAG ATAAGAAAGA GGACTTGATT
    TCTCCATGTA AAGTAAGTTA TGATGTAACA TCAGCAAGAG ATATGCTGCT GCTAGCGTGT
    TCTCAGGATG AACAAAAAAA ATGGGTAACT CATTTAGTAA AGAAGATCCC TAAGAATCCA
    CCATCTGGTT TTGTGCGTGC ATCCCCTCGA ACACTTTCTA CAAGGTCCAC TGCAAATCAG
    TCTTTCCGGA AAGTGGTCAA AAATACATCT GGAAAAGCTA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230093
    Clone ID Related Accession (Same CDS sequence) XM_024984560.1
    Accession Version XM_024984560.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4062bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho-associated protein kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GCGACAGTTT TGAGACTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCCAAATCGG AAGTGAATTC AGATTGTCTG CTGGATGGAT TGGATGCTTT GGTATATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGA ATGAAAGCTG AAGATTATGA AGTAGTGAAG
    GTGATTGGTA GAGGTGCATT TGGAGAAGTT CAATTGGTAA GGCATAAATC CACCAGGAAG
    GTGTACGCTA TGAAGCTCCT CAGCAAATTT GAAATGATTA AGAGATCTGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCTTTT GCTAACAGTC CTTGGGTTGT TCAGCTGTTT
    TATGCATTCC AAGATGATCG TTATCTTTAC ATGGTAATGG AGTACATGCC TGGTGGAGAT
    CTTGTAAATT TAATGAGCAA CTATGACGTG CCTGAAAAAT GGGCCCGATT CTATACTGCA
    GAAGTAGTTC TTGCATTGGA TGCAATCCAT TCCATGGGTT TTATCCATAG AGATGTGAAG
    CCTGATAACA TGCTGCTGGA TAAATCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG CATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGATTAT
    ATTTCCCCTG AAGTATTAAA ATCTCAAGGT GGCGATGGTT ATTATGGACG AGAATGTGAC
    TGGTGGTCAG TTGGGGTATT TTTATATGAA ATGCTTGTAG GTGATACACC ATTTTATGCA
    GATTCTCTGG TTGGAACTTA CAGTAAGATT ATGAACCATA AAAATTCTCT TACCTTCCCT
    GATGATAATG ACATATCAAA AGAAGCAAAA AATCTTATTT GTGCCTTCCT TACTGACAGA
    GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT
    GATCAATGGG CTTGGGAAAC ACTTCGAGAC ACTGTAGCAC CAGTTGTACC TGATTTAAGT
    AGTGACATAG ACACAAGTAA CTTTGATGAC TTGGAAGAAG ATAAAGGAGA TGAAGAAACA
    TTCCCTATAC CTAAAGCTTT TGTTGGCAAT CAACTACCTT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTATC TTCAGCAAAT CCTAACGATA ACAGAACTAG CTCTAATGTG
    GATAAAAGCT TGGAAAATTT GCAGAAAACA ATTTATAAGC TGGAAGAACA GTTACATAAT
    GAAATGCAAT TAAAAGATGA AATGGAGCAG AAGTGCAGAA CGTCAAACAT AAAACTAGAC
    AAGATAATGA AAGAATTGGA CGAAGAGGGG AATCAAAGAA GAAATCTAGA ATCTACAGTA
    TCTCAGATTG AGAAGGAGAA AATGTTGCTA CAGCACAGAA TTAATGAATA CCAAAGAAAA
    GCAGAACAGG AAAATGAGAA GAGGAGGAAT GTAGAAAATG AAGTTTCTAC ATTAAAGGAT
    CAGTTGGAAG ACTTAAAGAA AGTCAGCCAG AATTCACAGC TTGCTAATGA GAAGCTGGCA
    CAATTACAAA AGCAGCTAGA AGAAGCCAAT GACTTACTTA GGACAGAATC AGACACAGCG
    GTTAGATTAA GGAAGAGTCA CACAGAGATG AGCAAATCCA TCACTCAGCT AGAGTCCCTA
    AACAGAGAAC TGCAGGAGAG AAATAGAATT TTAGAGAATT CCAAGTCACA AATGGACAAA
    GATTATTACC AACTACAGGC TGTATTAGAA GCTGAACGAA GAGACAGAGG TCATGATTCT
    GAGAAGATTG GAGACCTACA AGCTCGAATT ACATCTTTAC AAGAGGAGGT GAAGCATCTC
    AAACATAATC TTGAAAGAGT GGAAGGAGAA AGAAAAGAGG CTCAAGACAT GCTTAATCAT
    TCAGAAAAGG AAAAGAATAA CTTAGAAATA GATTTAAACT ACAAGCTTAA ATCATTACAG
    CAACGATTAG AACAAGAGGT AAATGAACAT AAAGTAACCA AAGCTCGTTT AACTGACAAA
    CATCAATCTA TTGAAGAGGC AAAGTCTGTT GCAATGTGTG AGATGGAAAA AAAGCTGAAG
    GAGGAGAGAG ACGCTCGAGA GAAGGCTGAA AATAGGGTTG TTCAGATTGA GAAGCAGTGC
    TCTATGCTAG ATGTTGATCT GAAGCAATCT CAGCAGAAGC TGGAGCATTT GATTGAAAAT
    AAAGACAGGA TGGAGGATGA AGTTAAGAAT CTAACGCTGC AGCTGGAGCA GGAATCAAAC
    AAGCGACTTT TGTTACAAAA TGAATTGAAG ACTCAAGCAT TTGAGGCAGA CAATTTAAAA
    GGTTTAGAAA AGCAGATGAA ACAGGAAATA AATACTTTAT TGGAAGCAAA GAGATTGTTG
    GAATTTGAAT TAGCTCAGCT TACAAAACAG TACAGAGGGA ATGAAGGGCA GATGCGGGAG
    CTGCAGGATC AGCTGGAAGC GGAGCAGTAT TTCTCGACAC TTTATAAAAC CCAGGTAAAG
    GAACTTAAGG AAGAAATTGA AGAAAAAAAC AGAGAAAACT TAAAGAAAAT ACAGGAACTA
    CAAAGTGAAA AAGAAACTCT TGCTACTCAG TTGGATCTAG CAGAAACAAA GGCTGAATCT
    GAGCAGTTGG CTCGAGGGCT TCTAGAAGAA CAGTATTTTG AACTGACCCA AGAAAGCAAG
    AAAGCTGCCT CAAGAAATAG ACAAGAGATT ACAGATAAAG ATCACACTCT TAGTCGGCTT
    GAAGAAACGA ATAGCATGCT AACCAAAGAT ATTGAATTAT TAAGAAAAGA AAACGAAGAG
    CTAACTGATA AAATGAGGAA GGCAGAGGAA GAATATAAAT TGAAGAAGGA GGAGGAGATC
    AATATCCTTA AGGCTGCCTT TGAAAAGAAT ATCAATACAG AACGAACCCT TAAAACACAG
    GCTGTTAACA AGTTGGCAGA AATAATGAAT CGAAAGGACT TTAAAATTGA TAGAAAGAAA
    GCAAATACGC AGGATTTGAG AAAGAAAGAA AAAGAAAATC GAAAGCTCCA ACTTGAACTC
    AACCAGGAAA GAGAGAAATT CAACCAAATG GTAGTGAAAC ATCAGAAGGA GCTGAATGAA
    ATGCAAGCGC AATTGGTAGA AGAATGTACA CATAAGAATG AGCTTCAGAT GCAGCTGGCC
    AGCAAGGAGA GTGACATTGA GCAGCTGCGT GCCAAACTGT TGGACCTTTC AGATTCTACA
    AGTGTTGCTA GTTTTCCCAG TGCTGATGAA ACTGATGGAA ACCTTCCAGA ATCAAGAATT
    GAAGGTTGGC TTTCAATACC AAATAGAGGA AATATCAAAA GATATGGCTG GAAGAAACAG
    TATGTTGTGG TAAGCAGCAA AAAAATTTTG TTCTATAATG ATGAACAAGA TAAGGAACAA
    TCCAATCCGT CTATGGTATT GGACATTGAT AAATTGTTTC ATGTTCGTCC TGTAACACAA
    GGAGATGTAT ACAGAGCTGA AACTGAAGAA ATTCCTAAAA TATTCCAGAT ACTGTATGCA
    AATGAAGGTG AATGTAGAAA AGACTTAGAG GTGGAACCAG TACAACAAGC TGAAAAAACA
    AATTTTCAAA ACCACAAAGG CCATGAGTTC ATTCCTACAC TCTACCACTT TCCCGCCAAC
    TGTGAGGCCT GTGCCAAACC TCTCTGGCAT GTTTTTAAGC CACCCCCTGC CCTGGAGTGT
    CGAAGATGCC ATGTTAAGTG CCACAGAGAC CACATAGATA AGAAAGAGGA CTTGATTTCT
    CCATGTAAAG TAAGTTATGA TGTAACATCA GCAAGAGATA TGCTGCTGCT AGCGTGTTCT
    CAGGATGAAC AAAAAAAATG GGTAACTCAT TTAGTAAAGA AGATCCCTAA GAATCCACCA
    TCTGGTTTTG TGCGTGCATC CCCTCGAACA CTTTCTACAA GGTCCACTGC AAATCAGTCT
    TTCCGGAAAG TGGTCAAAAA TACATCTGGA AAAGCTAGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840328.1
    CDS151..4212
    Translation

    Target ORF information:

    RefSeq Version XM_024984560.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984560.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTCGACTG GCGACAGTTT TGAGACTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 
    CCCAAATCGG AAGTGAATTC AGATTGTCTG CTGGATGGAT TGGATGCTTT GGTATATGAT
    TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAGATATAAA
    GACACAATAA ATAAAATTAG AGATTTACGA ATGAAAGCTG AAGATTATGA AGTAGTGAAG
    GTGATTGGTA GAGGTGCATT TGGAGAAGTT CAATTGGTAA GGCATAAATC CACCAGGAAG
    GTGTACGCTA TGAAGCTCCT CAGCAAATTT GAAATGATTA AGAGATCTGA TTCTGCTTTT
    TTCTGGGAAG AAAGGGACAT CATGGCTTTT GCTAACAGTC CTTGGGTTGT TCAGCTGTTT
    TATGCATTCC AAGATGATCG TTATCTTTAC ATGGTAATGG AGTACATGCC TGGTGGAGAT
    CTTGTAAATT TAATGAGCAA CTATGACGTG CCTGAAAAAT GGGCCCGATT CTATACTGCA
    GAAGTAGTTC TTGCATTGGA TGCAATCCAT TCCATGGGTT TTATCCATAG AGATGTGAAG
    CCTGATAACA TGCTGCTGGA TAAATCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT
    ATGAAGATGA ATAAGGAAGG CATGGTACGA TGTGATACAG CAGTTGGAAC ACCTGATTAT
    ATTTCCCCTG AAGTATTAAA ATCTCAAGGT GGCGATGGTT ATTATGGACG AGAATGTGAC
    TGGTGGTCAG TTGGGGTATT TTTATATGAA ATGCTTGTAG GTGATACACC ATTTTATGCA
    GATTCTCTGG TTGGAACTTA CAGTAAGATT ATGAACCATA AAAATTCTCT TACCTTCCCT
    GATGATAATG ACATATCAAA AGAAGCAAAA AATCTTATTT GTGCCTTCCT TACTGACAGA
    GAGGTGAGAT TAGGGCGAAA TGGTGTAGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT
    GATCAATGGG CTTGGGAAAC ACTTCGAGAC ACTGTAGCAC CAGTTGTACC TGATTTAAGT
    AGTGACATAG ACACAAGTAA CTTTGATGAC TTGGAAGAAG ATAAAGGAGA TGAAGAAACA
    TTCCCTATAC CTAAAGCTTT TGTTGGCAAT CAACTACCTT TTGTAGGATT TACATATTAT
    AGCAATCGTA GATACTTATC TTCAGCAAAT CCTAACGATA ACAGAACTAG CTCTAATGTG
    GATAAAAGCT TGGAAAATTT GCAGAAAACA ATTTATAAGC TGGAAGAACA GTTACATAAT
    GAAATGCAAT TAAAAGATGA AATGGAGCAG AAGTGCAGAA CGTCAAACAT AAAACTAGAC
    AAGATAATGA AAGAATTGGA CGAAGAGGGG AATCAAAGAA GAAATCTAGA ATCTACAGTA
    TCTCAGATTG AGAAGGAGAA AATGTTGCTA CAGCACAGAA TTAATGAATA CCAAAGAAAA
    GCAGAACAGG AAAATGAGAA GAGGAGGAAT GTAGAAAATG AAGTTTCTAC ATTAAAGGAT
    CAGTTGGAAG ACTTAAAGAA AGTCAGCCAG AATTCACAGC TTGCTAATGA GAAGCTGGCA
    CAATTACAAA AGCAGCTAGA AGAAGCCAAT GACTTACTTA GGACAGAATC AGACACAGCG
    GTTAGATTAA GGAAGAGTCA CACAGAGATG AGCAAATCCA TCACTCAGCT AGAGTCCCTA
    AACAGAGAAC TGCAGGAGAG AAATAGAATT TTAGAGAATT CCAAGTCACA AATGGACAAA
    GATTATTACC AACTACAGGC TGTATTAGAA GCTGAACGAA GAGACAGAGG TCATGATTCT
    GAGAAGATTG GAGACCTACA AGCTCGAATT ACATCTTTAC AAGAGGAGGT GAAGCATCTC
    AAACATAATC TTGAAAGAGT GGAAGGAGAA AGAAAAGAGG CTCAAGACAT GCTTAATCAT
    TCAGAAAAGG AAAAGAATAA CTTAGAAATA GATTTAAACT ACAAGCTTAA ATCATTACAG
    CAACGATTAG AACAAGAGGT AAATGAACAT AAAGTAACCA AAGCTCGTTT AACTGACAAA
    CATCAATCTA TTGAAGAGGC AAAGTCTGTT GCAATGTGTG AGATGGAAAA AAAGCTGAAG
    GAGGAGAGAG ACGCTCGAGA GAAGGCTGAA AATAGGGTTG TTCAGATTGA GAAGCAGTGC
    TCTATGCTAG ATGTTGATCT GAAGCAATCT CAGCAGAAGC TGGAGCATTT GATTGAAAAT
    AAAGACAGGA TGGAGGATGA AGTTAAGAAT CTAACGCTGC AGCTGGAGCA GGAATCAAAC
    AAGCGACTTT TGTTACAAAA TGAATTGAAG ACTCAAGCAT TTGAGGCAGA CAATTTAAAA
    GGTTTAGAAA AGCAGATGAA ACAGGAAATA AATACTTTAT TGGAAGCAAA GAGATTGTTG
    GAATTTGAAT TAGCTCAGCT TACAAAACAG TACAGAGGGA ATGAAGGGCA GATGCGGGAG
    CTGCAGGATC AGCTGGAAGC GGAGCAGTAT TTCTCGACAC TTTATAAAAC CCAGGTAAAG
    GAACTTAAGG AAGAAATTGA AGAAAAAAAC AGAGAAAACT TAAAGAAAAT ACAGGAACTA
    CAAAGTGAAA AAGAAACTCT TGCTACTCAG TTGGATCTAG CAGAAACAAA GGCTGAATCT
    GAGCAGTTGG CTCGAGGGCT TCTAGAAGAA CAGTATTTTG AACTGACCCA AGAAAGCAAG
    AAAGCTGCCT CAAGAAATAG ACAAGAGATT ACAGATAAAG ATCACACTCT TAGTCGGCTT
    GAAGAAACGA ATAGCATGCT AACCAAAGAT ATTGAATTAT TAAGAAAAGA AAACGAAGAG
    CTAACTGATA AAATGAGGAA GGCAGAGGAA GAATATAAAT TGAAGAAGGA GGAGGAGATC
    AATATCCTTA AGGCTGCCTT TGAAAAGAAT ATCAATACAG AACGAACCCT TAAAACACAG
    GCTGTTAACA AGTTGGCAGA AATAATGAAT CGAAAGGACT TTAAAATTGA TAGAAAGAAA
    GCAAATACGC AGGATTTGAG AAAGAAAGAA AAAGAAAATC GAAAGCTCCA ACTTGAACTC
    AACCAGGAAA GAGAGAAATT CAACCAAATG GTAGTGAAAC ATCAGAAGGA GCTGAATGAA
    ATGCAAGCGC AATTGGTAGA AGAATGTACA CATAAGAATG AGCTTCAGAT GCAGCTGGCC
    AGCAAGGAGA GTGACATTGA GCAGCTGCGT GCCAAACTGT TGGACCTTTC AGATTCTACA
    AGTGTTGCTA GTTTTCCCAG TGCTGATGAA ACTGATGGAA ACCTTCCAGA ATCAAGAATT
    GAAGGTTGGC TTTCAATACC AAATAGAGGA AATATCAAAA GATATGGCTG GAAGAAACAG
    TATGTTGTGG TAAGCAGCAA AAAAATTTTG TTCTATAATG ATGAACAAGA TAAGGAACAA
    TCCAATCCGT CTATGGTATT GGACATTGAT AAATTGTTTC ATGTTCGTCC TGTAACACAA
    GGAGATGTAT ACAGAGCTGA AACTGAAGAA ATTCCTAAAA TATTCCAGAT ACTGTATGCA
    AATGAAGGTG AATGTAGAAA AGACTTAGAG GTGGAACCAG TACAACAAGC TGAAAAAACA
    AATTTTCAAA ACCACAAAGG CCATGAGTTC ATTCCTACAC TCTACCACTT TCCCGCCAAC
    TGTGAGGCCT GTGCCAAACC TCTCTGGCAT GTTTTTAAGC CACCCCCTGC CCTGGAGTGT
    CGAAGATGCC ATGTTAAGTG CCACAGAGAC CACATAGATA AGAAAGAGGA CTTGATTTCT
    CCATGTAAAG TAAGTTATGA TGTAACATCA GCAAGAGATA TGCTGCTGCT AGCGTGTTCT
    CAGGATGAAC AAAAAAAATG GGTAACTCAT TTAGTAAAGA AGATCCCTAA GAATCCACCA
    TCTGGTTTTG TGCGTGCATC CCCTCGAACA CTTTCTACAA GGTCCACTGC AAATCAGTCT
    TTCCGGAAAG TGGTCAAAAA TACATCTGGA AAAGCTAGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88329
    Clone ID Related Accession (Same CDS sequence) XM_002697789.5 , XM_015469135.1
    Accession Version XM_002697789.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3384bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho-associated protein kinase 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002697789.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTACGAT GTGATACAGC AGTTGGAACA CCTGATTATA TTTCCCCTGA AGTATTAAAA 
    TCTCAAGGTG GCGATGGTTA TTATGGACGA GAATGTGACT GGTGGTCAGT TGGGGTATTT
    TTATATGAAA TGCTTGTAGG TGATACACCA TTTTATGCAG ATTCTCTGGT TGGAACTTAC
    AGTAAGATTA TGAACCATAA AAATTCTCTT ACCTTCCCTG ATGATAATGA CATATCAAAA
    GAAGCAAAAA ATCTTATTTG TGCCTTCCTT ACTGACAGAG AGGTGAGATT AGGGCGAAAT
    GGTGTAGAAG AAATCAAACG ACATCTCTTC TTCAAAAATG ATCAATGGGC TTGGGAAACA
    CTTCGAGACA CTGTAGCACC AGTTGTACCT GATTTAAGTA GTGACATAGA CACAAGTAAC
    TTTGATGACT TGGAAGAAGA TAAAGGAGAT GAAGAAACAT TCCCTATACC TAAAGCTTTT
    GTTGGCAATC AACTACCTTT TGTAGGATTT ACATATTATA GCAATCGTAG ATACTTATCT
    TCAGCAAATC CTAACGATAA CAGAACTAGC TCTAATGTGG ATAAAAGCTT GCAGGAAAAT
    TTGCAGAAAA CAATTTATAA GCTGGAAGAA CAGTTACATA ATGAAATGCA ATTAAAAGAT
    GAAATGGAGC AGAAGTGCAG AACGTCAAAC ATAAAACTAG ACAAGATAAT GAAAGAATTG
    GACGAAGAGG GGAATCAAAG AAGAAATCTA GAATCTACAG TATCTCAGAT TGAGAAGGAG
    AAAATGTTGC TACAGCACAG AATTAATGAA TACCAAAGAA AAGCAGAACA GGAAAATGAG
    AAGAGGAGGA ATGTAGAAAA TGAAGTTTCT ACATTAAAGG ATCAGTTGGA AGACTTAAAG
    AAAGTCAGCC AGAATTCACA GCTTGCTAAT GAGAAGCTGG CACAATTACA AAAGCAGCTA
    GAAGAAGCCA ATGACTTACT TAGGACAGAA TCAGACACAG CGGTTAGATT AAGGAAGAGT
    CACACAGAGA TGAGCAAATC CATCACTCAG CTAGAGTCCC TAAACAGAGA ACTGCAGGAG
    AGAAATAGAA TTTTAGAGAA TTCCAAGTCA CAAATGGACA AAGATTATTA CCAACTACAG
    GCTGTATTAG AAGCTGAACG AAGAGACAGA GGTCATGATT CTGAGAAGAT TGGAGACCTA
    CAAGCTCGAA TTACATCTTT ACAAGAGGAG GTGAAGCATC TCAAACATAA TCTTGAAAGA
    GTGGAAGGAG AAAGAAAAGA GGCTCAAGAC ATGCTTAATC ATTCAGAAAA GGAAAAGAAT
    AACTTAGAAA TAGATTTAAA CTACAAGCTT AAATCATTAC AGCAACGATT AGAACAAGAG
    GTAAATGAAC ATAAAGTAAC CAAAGCTCGT TTAACTGACA AACATCAATC TATTGAAGAG
    GCAAAGTCTG TTGCAATGTG TGAGATGGAA AAAAAGCTGA AGGAGGAGAG AGACGCTCGA
    GAGAAGGCTG AAAATAGGGT TGTTCAGATT GAGAAGCAGT GCTCTATGCT AGATGTTGAT
    CTGAAGCAAT CTCAGCAGAA GCTGGAGCAT TTGATTGAAA ATAAAGACAG GATGGAGGAT
    GAAGTTAAGA ATCTAACGCT GCAGCTGGAG CAGGAATCAA ACAAGCGACT TTTGTTACAA
    AATGAATTGA AGACTCAAGC ATTTGAGGCA GACAATTTAA AAGGTTTAGA AAAGCAGATG
    AAACAGGAAA TAAATACTTT ATTGGAAGCA AAGAGATTGT TGGAATTTGA ATTAGCTCAG
    CTTACAAAAC AGTACAGAGG GAATGAAGGG CAGATGCGGG AGCTGCAGGA TCAGCTGGAA
    GCGGAGCAGT ATTTCTCGAC ACTTTATAAA ACCCAGGTAA AGGAACTTAA GGAAGAAATT
    GAAGAAAAAA ACAGAGAAAA CTTAAAGAAA ATACAGGAAC TACAAAGTGA AAAAGAAACT
    CTTGCTACTC AGTTGGATCT AGCAGAAACA AAGGCTGAAT CTGAGCAGTT GGCTCGAGGG
    CTTCTAGAAG AACAGTATTT TGAACTGACC CAAGAAAGCA AGAAAGCTGC CTCAAGAAAT
    AGACAAGAGA TTACAGATAA AGATCACACT CTTAGTCGGC TTGAAGAAAC GAATAGCATG
    CTAACCAAAG ATATTGAATT ATTAAGAAAA GAAAACGAAG AGCTAACTGA TAAAATGAGG
    AAGGCAGAGG AAGAATATAA ATTGAAGAAG GAGGAGGAGA TCAATATCCT TAAGGCTGCC
    TTTGAAAAGA ATATCAATAC AGAACGAACC CTTAAAACAC AGGCTGTTAA CAAGTTGGCA
    GAAATAATGA ATCGAAAGGA CTTTAAAATT GATAGAAAGA AAGCAAATAC GCAGGATTTG
    AGAAAGAAAG AAAAAGAAAA TCGAAAGCTC CAACTTGAAC TCAACCAGGA AAGAGAGAAA
    TTCAACCAAA TGGTAGTGAA ACATCAGAAG GAGCTGAATG AAATGCAAGC GCAATTGGTA
    GAAGAATGTA CACATAAGAA TGAGCTTCAG ATGCAGCTGG CCAGCAAGGA GAGTGACATT
    GAGCAGCTGC GTGCCAAACT GTTGGACCTT TCAGATTCTA CAAGTGTTGC TAGTTTTCCC
    AGTGCTGATG AAACTGATGG AAACCTTCCA GAATCAAGAA TTGAAGGTTG GCTTTCAATA
    CCAAATAGAG GAAATATCAA AAGATATGGC TGGAAGAAAC AGTATGTTGT GGTAAGCAGC
    AAAAAAATTT TGTTCTATAA TGATGAACAA GATAAGGAAC AATCCAATCC GTCTATGGTA
    TTGGACATTG ATAAATTGTT TCATGTTCGT CCTGTAACAC AAGGAGATGT ATACAGAGCT
    GAAACTGAAG AAATTCCTAA AATATTCCAG ATACTGTATG CAAATGAAGG TGAATGTAGA
    AAAGACTTAG AGGTGGAACC AGTACAACAA GCTGAAAAAA CAAATTTTCA AAACCACAAA
    GGCCATGAGT TCATTCCTAC ACTCTACCAC TTTCCCGCCA ACTGTGAGGC CTGTGCCAAA
    CCTCTCTGGC ATGTTTTTAA GCCACCCCCT GCCCTGGAGT GTCGAAGATG CCATGTTAAG
    TGCCACAGAG ACCACATAGA TAAGAAAGAG GACTTGATTT CTCCATGTAA AGTAAGTTAT
    GATGTAACAT CAGCAAGAGA TATGCTGCTG CTAGCGTGTT CTCAGGATGA ACAAAAAAAA
    TGGGTAACTC ATTTAGTAAA GAAGATCCCT AAGAATCCAC CATCTGGTTT TGTGCGTGCA
    TCCCCTCGAA CACTTTCTAC AAGGTCCACT GCAAATCAGT CTTTCCGGAA AGTGGTCAAA
    AATACATCTG GAAAAGCTAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002697835.4
    CDS32..3415
    Translation

    Target ORF information:

    RefSeq Version XM_002697789.5
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho associated coiled-coil containing protein kinase 1 (ROCK1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002697789.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTACGAT GTGATACAGC AGTTGGAACA CCTGATTATA TTTCCCCTGA AGTATTAAAA 
    TCTCAAGGTG GCGATGGTTA TTATGGACGA GAATGTGACT GGTGGTCAGT TGGGGTATTT
    TTATATGAAA TGCTTGTAGG TGATACACCA TTTTATGCAG ATTCTCTGGT TGGAACTTAC
    AGTAAGATTA TGAACCATAA AAATTCTCTT ACCTTCCCTG ATGATAATGA CATATCAAAA
    GAAGCAAAAA ATCTTATTTG TGCCTTCCTT ACTGACAGAG AGGTGAGATT AGGGCGAAAT
    GGTGTAGAAG AAATCAAACG ACATCTCTTC TTCAAAAATG ATCAATGGGC TTGGGAAACA
    CTTCGAGACA CTGTAGCACC AGTTGTACCT GATTTAAGTA GTGACATAGA CACAAGTAAC
    TTTGATGACT TGGAAGAAGA TAAAGGAGAT GAAGAAACAT TCCCTATACC TAAAGCTTTT
    GTTGGCAATC AACTACCTTT TGTAGGATTT ACATATTATA GCAATCGTAG ATACTTATCT
    TCAGCAAATC CTAACGATAA CAGAACTAGC TCTAATGTGG ATAAAAGCTT GCAGGAAAAT
    TTGCAGAAAA CAATTTATAA GCTGGAAGAA CAGTTACATA ATGAAATGCA ATTAAAAGAT
    GAAATGGAGC AGAAGTGCAG AACGTCAAAC ATAAAACTAG ACAAGATAAT GAAAGAATTG
    GACGAAGAGG GGAATCAAAG AAGAAATCTA GAATCTACAG TATCTCAGAT TGAGAAGGAG
    AAAATGTTGC TACAGCACAG AATTAATGAA TACCAAAGAA AAGCAGAACA GGAAAATGAG
    AAGAGGAGGA ATGTAGAAAA TGAAGTTTCT ACATTAAAGG ATCAGTTGGA AGACTTAAAG
    AAAGTCAGCC AGAATTCACA GCTTGCTAAT GAGAAGCTGG CACAATTACA AAAGCAGCTA
    GAAGAAGCCA ATGACTTACT TAGGACAGAA TCAGACACAG CGGTTAGATT AAGGAAGAGT
    CACACAGAGA TGAGCAAATC CATCACTCAG CTAGAGTCCC TAAACAGAGA ACTGCAGGAG
    AGAAATAGAA TTTTAGAGAA TTCCAAGTCA CAAATGGACA AAGATTATTA CCAACTACAG
    GCTGTATTAG AAGCTGAACG AAGAGACAGA GGTCATGATT CTGAGAAGAT TGGAGACCTA
    CAAGCTCGAA TTACATCTTT ACAAGAGGAG GTGAAGCATC TCAAACATAA TCTTGAAAGA
    GTGGAAGGAG AAAGAAAAGA GGCTCAAGAC ATGCTTAATC ATTCAGAAAA GGAAAAGAAT
    AACTTAGAAA TAGATTTAAA CTACAAGCTT AAATCATTAC AGCAACGATT AGAACAAGAG
    GTAAATGAAC ATAAAGTAAC CAAAGCTCGT TTAACTGACA AACATCAATC TATTGAAGAG
    GCAAAGTCTG TTGCAATGTG TGAGATGGAA AAAAAGCTGA AGGAGGAGAG AGACGCTCGA
    GAGAAGGCTG AAAATAGGGT TGTTCAGATT GAGAAGCAGT GCTCTATGCT AGATGTTGAT
    CTGAAGCAAT CTCAGCAGAA GCTGGAGCAT TTGATTGAAA ATAAAGACAG GATGGAGGAT
    GAAGTTAAGA ATCTAACGCT GCAGCTGGAG CAGGAATCAA ACAAGCGACT TTTGTTACAA
    AATGAATTGA AGACTCAAGC ATTTGAGGCA GACAATTTAA AAGGTTTAGA AAAGCAGATG
    AAACAGGAAA TAAATACTTT ATTGGAAGCA AAGAGATTGT TGGAATTTGA ATTAGCTCAG
    CTTACAAAAC AGTACAGAGG GAATGAAGGG CAGATGCGGG AGCTGCAGGA TCAGCTGGAA
    GCGGAGCAGT ATTTCTCGAC ACTTTATAAA ACCCAGGTAA AGGAACTTAA GGAAGAAATT
    GAAGAAAAAA ACAGAGAAAA CTTAAAGAAA ATACAGGAAC TACAAAGTGA AAAAGAAACT
    CTTGCTACTC AGTTGGATCT AGCAGAAACA AAGGCTGAAT CTGAGCAGTT GGCTCGAGGG
    CTTCTAGAAG AACAGTATTT TGAACTGACC CAAGAAAGCA AGAAAGCTGC CTCAAGAAAT
    AGACAAGAGA TTACAGATAA AGATCACACT CTTAGTCGGC TTGAAGAAAC GAATAGCATG
    CTAACCAAAG ATATTGAATT ATTAAGAAAA GAAAACGAAG AGCTAACTGA TAAAATGAGG
    AAGGCAGAGG AAGAATATAA ATTGAAGAAG GAGGAGGAGA TCAATATCCT TAAGGCTGCC
    TTTGAAAAGA ATATCAATAC AGAACGAACC CTTAAAACAC AGGCTGTTAA CAAGTTGGCA
    GAAATAATGA ATCGAAAGGA CTTTAAAATT GATAGAAAGA AAGCAAATAC GCAGGATTTG
    AGAAAGAAAG AAAAAGAAAA TCGAAAGCTC CAACTTGAAC TCAACCAGGA AAGAGAGAAA
    TTCAACCAAA TGGTAGTGAA ACATCAGAAG GAGCTGAATG AAATGCAAGC GCAATTGGTA
    GAAGAATGTA CACATAAGAA TGAGCTTCAG ATGCAGCTGG CCAGCAAGGA GAGTGACATT
    GAGCAGCTGC GTGCCAAACT GTTGGACCTT TCAGATTCTA CAAGTGTTGC TAGTTTTCCC
    AGTGCTGATG AAACTGATGG AAACCTTCCA GAATCAAGAA TTGAAGGTTG GCTTTCAATA
    CCAAATAGAG GAAATATCAA AAGATATGGC TGGAAGAAAC AGTATGTTGT GGTAAGCAGC
    AAAAAAATTT TGTTCTATAA TGATGAACAA GATAAGGAAC AATCCAATCC GTCTATGGTA
    TTGGACATTG ATAAATTGTT TCATGTTCGT CCTGTAACAC AAGGAGATGT ATACAGAGCT
    GAAACTGAAG AAATTCCTAA AATATTCCAG ATACTGTATG CAAATGAAGG TGAATGTAGA
    AAAGACTTAG AGGTGGAACC AGTACAACAA GCTGAAAAAA CAAATTTTCA AAACCACAAA
    GGCCATGAGT TCATTCCTAC ACTCTACCAC TTTCCCGCCA ACTGTGAGGC CTGTGCCAAA
    CCTCTCTGGC ATGTTTTTAA GCCACCCCCT GCCCTGGAGT GTCGAAGATG CCATGTTAAG
    TGCCACAGAG ACCACATAGA TAAGAAAGAG GACTTGATTT CTCCATGTAA AGTAAGTTAT
    GATGTAACAT CAGCAAGAGA TATGCTGCTG CTAGCGTGTT CTCAGGATGA ACAAAAAAAA
    TGGGTAACTC ATTTAGTAAA GAAGATCCCT AAGAATCCAC CATCTGGTTT TGTGCGTGCA
    TCCCCTCGAA CACTTTCTAC AAGGTCCACT GCAAATCAGT CTTTCCGGAA AGTGGTCAAA
    AATACATCTG GAAAAGCTAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88329
    Clone ID Related Accession (Same CDS sequence) XM_002697789.5 , XM_015469135.1
    Accession Version XM_015469135.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3384bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho-associated protein kinase 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTACGAT GTGATACAGC AGTTGGAACA CCTGATTATA TTTCCCCTGA AGTATTAAAA 
    TCTCAAGGTG GCGATGGTTA TTATGGACGA GAATGTGACT GGTGGTCAGT TGGGGTATTT
    TTATATGAAA TGCTTGTAGG TGATACACCA TTTTATGCAG ATTCTCTGGT TGGAACTTAC
    AGTAAGATTA TGAACCATAA AAATTCTCTT ACCTTCCCTG ATGATAATGA CATATCAAAA
    GAAGCAAAAA ATCTTATTTG TGCCTTCCTT ACTGACAGAG AGGTGAGATT AGGGCGAAAT
    GGTGTAGAAG AAATCAAACG ACATCTCTTC TTCAAAAATG ATCAATGGGC TTGGGAAACA
    CTTCGAGACA CTGTAGCACC AGTTGTACCT GATTTAAGTA GTGACATAGA CACAAGTAAC
    TTTGATGACT TGGAAGAAGA TAAAGGAGAT GAAGAAACAT TCCCTATACC TAAAGCTTTT
    GTTGGCAATC AACTACCTTT TGTAGGATTT ACATATTATA GCAATCGTAG ATACTTATCT
    TCAGCAAATC CTAACGATAA CAGAACTAGC TCTAATGTGG ATAAAAGCTT GCAGGAAAAT
    TTGCAGAAAA CAATTTATAA GCTGGAAGAA CAGTTACATA ATGAAATGCA ATTAAAAGAT
    GAAATGGAGC AGAAGTGCAG AACGTCAAAC ATAAAACTAG ACAAGATAAT GAAAGAATTG
    GACGAAGAGG GGAATCAAAG AAGAAATCTA GAATCTACAG TATCTCAGAT TGAGAAGGAG
    AAAATGTTGC TACAGCACAG AATTAATGAA TACCAAAGAA AAGCAGAACA GGAAAATGAG
    AAGAGGAGGA ATGTAGAAAA TGAAGTTTCT ACATTAAAGG ATCAGTTGGA AGACTTAAAG
    AAAGTCAGCC AGAATTCACA GCTTGCTAAT GAGAAGCTGG CACAATTACA AAAGCAGCTA
    GAAGAAGCCA ATGACTTACT TAGGACAGAA TCAGACACAG CGGTTAGATT AAGGAAGAGT
    CACACAGAGA TGAGCAAATC CATCACTCAG CTAGAGTCCC TAAACAGAGA ACTGCAGGAG
    AGAAATAGAA TTTTAGAGAA TTCCAAGTCA CAAATGGACA AAGATTATTA CCAACTACAG
    GCTGTATTAG AAGCTGAACG AAGAGACAGA GGTCATGATT CTGAGAAGAT TGGAGACCTA
    CAAGCTCGAA TTACATCTTT ACAAGAGGAG GTGAAGCATC TCAAACATAA TCTTGAAAGA
    GTGGAAGGAG AAAGAAAAGA GGCTCAAGAC ATGCTTAATC ATTCAGAAAA GGAAAAGAAT
    AACTTAGAAA TAGATTTAAA CTACAAGCTT AAATCATTAC AGCAACGATT AGAACAAGAG
    GTAAATGAAC ATAAAGTAAC CAAAGCTCGT TTAACTGACA AACATCAATC TATTGAAGAG
    GCAAAGTCTG TTGCAATGTG TGAGATGGAA AAAAAGCTGA AGGAGGAGAG AGACGCTCGA
    GAGAAGGCTG AAAATAGGGT TGTTCAGATT GAGAAGCAGT GCTCTATGCT AGATGTTGAT
    CTGAAGCAAT CTCAGCAGAA GCTGGAGCAT TTGATTGAAA ATAAAGACAG GATGGAGGAT
    GAAGTTAAGA ATCTAACGCT GCAGCTGGAG CAGGAATCAA ACAAGCGACT TTTGTTACAA
    AATGAATTGA AGACTCAAGC ATTTGAGGCA GACAATTTAA AAGGTTTAGA AAAGCAGATG
    AAACAGGAAA TAAATACTTT ATTGGAAGCA AAGAGATTGT TGGAATTTGA ATTAGCTCAG
    CTTACAAAAC AGTACAGAGG GAATGAAGGG CAGATGCGGG AGCTGCAGGA TCAGCTGGAA
    GCGGAGCAGT ATTTCTCGAC ACTTTATAAA ACCCAGGTAA AGGAACTTAA GGAAGAAATT
    GAAGAAAAAA ACAGAGAAAA CTTAAAGAAA ATACAGGAAC TACAAAGTGA AAAAGAAACT
    CTTGCTACTC AGTTGGATCT AGCAGAAACA AAGGCTGAAT CTGAGCAGTT GGCTCGAGGG
    CTTCTAGAAG AACAGTATTT TGAACTGACC CAAGAAAGCA AGAAAGCTGC CTCAAGAAAT
    AGACAAGAGA TTACAGATAA AGATCACACT CTTAGTCGGC TTGAAGAAAC GAATAGCATG
    CTAACCAAAG ATATTGAATT ATTAAGAAAA GAAAACGAAG AGCTAACTGA TAAAATGAGG
    AAGGCAGAGG AAGAATATAA ATTGAAGAAG GAGGAGGAGA TCAATATCCT TAAGGCTGCC
    TTTGAAAAGA ATATCAATAC AGAACGAACC CTTAAAACAC AGGCTGTTAA CAAGTTGGCA
    GAAATAATGA ATCGAAAGGA CTTTAAAATT GATAGAAAGA AAGCAAATAC GCAGGATTTG
    AGAAAGAAAG AAAAAGAAAA TCGAAAGCTC CAACTTGAAC TCAACCAGGA AAGAGAGAAA
    TTCAACCAAA TGGTAGTGAA ACATCAGAAG GAGCTGAATG AAATGCAAGC GCAATTGGTA
    GAAGAATGTA CACATAAGAA TGAGCTTCAG ATGCAGCTGG CCAGCAAGGA GAGTGACATT
    GAGCAGCTGC GTGCCAAACT GTTGGACCTT TCAGATTCTA CAAGTGTTGC TAGTTTTCCC
    AGTGCTGATG AAACTGATGG AAACCTTCCA GAATCAAGAA TTGAAGGTTG GCTTTCAATA
    CCAAATAGAG GAAATATCAA AAGATATGGC TGGAAGAAAC AGTATGTTGT GGTAAGCAGC
    AAAAAAATTT TGTTCTATAA TGATGAACAA GATAAGGAAC AATCCAATCC GTCTATGGTA
    TTGGACATTG ATAAATTGTT TCATGTTCGT CCTGTAACAC AAGGAGATGT ATACAGAGCT
    GAAACTGAAG AAATTCCTAA AATATTCCAG ATACTGTATG CAAATGAAGG TGAATGTAGA
    AAAGACTTAG AGGTGGAACC AGTACAACAA GCTGAAAAAA CAAATTTTCA AAACCACAAA
    GGCCATGAGT TCATTCCTAC ACTCTACCAC TTTCCCGCCA ACTGTGAGGC CTGTGCCAAA
    CCTCTCTGGC ATGTTTTTAA GCCACCCCCT GCCCTGGAGT GTCGAAGATG CCATGTTAAG
    TGCCACAGAG ACCACATAGA TAAGAAAGAG GACTTGATTT CTCCATGTAA AGTAAGTTAT
    GATGTAACAT CAGCAAGAGA TATGCTGCTG CTAGCGTGTT CTCAGGATGA ACAAAAAAAA
    TGGGTAACTC ATTTAGTAAA GAAGATCCCT AAGAATCCAC CATCTGGTTT TGTGCGTGCA
    TCCCCTCGAA CACTTTCTAC AAGGTCCACT GCAAATCAGT CTTTCCGGAA AGTGGTCAAA
    AATACATCTG GAAAAGCTAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324621.1
    CDS32..3415
    Translation

    Target ORF information:

    RefSeq Version XM_015469135.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho associated coiled-coil containing protein kinase 1 (ROCK1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469135.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTACGAT GTGATACAGC AGTTGGAACA CCTGATTATA TTTCCCCTGA AGTATTAAAA 
    TCTCAAGGTG GCGATGGTTA TTATGGACGA GAATGTGACT GGTGGTCAGT TGGGGTATTT
    TTATATGAAA TGCTTGTAGG TGATACACCA TTTTATGCAG ATTCTCTGGT TGGAACTTAC
    AGTAAGATTA TGAACCATAA AAATTCTCTT ACCTTCCCTG ATGATAATGA CATATCAAAA
    GAAGCAAAAA ATCTTATTTG TGCCTTCCTT ACTGACAGAG AGGTGAGATT AGGGCGAAAT
    GGTGTAGAAG AAATCAAACG ACATCTCTTC TTCAAAAATG ATCAATGGGC TTGGGAAACA
    CTTCGAGACA CTGTAGCACC AGTTGTACCT GATTTAAGTA GTGACATAGA CACAAGTAAC
    TTTGATGACT TGGAAGAAGA TAAAGGAGAT GAAGAAACAT TCCCTATACC TAAAGCTTTT
    GTTGGCAATC AACTACCTTT TGTAGGATTT ACATATTATA GCAATCGTAG ATACTTATCT
    TCAGCAAATC CTAACGATAA CAGAACTAGC TCTAATGTGG ATAAAAGCTT GCAGGAAAAT
    TTGCAGAAAA CAATTTATAA GCTGGAAGAA CAGTTACATA ATGAAATGCA ATTAAAAGAT
    GAAATGGAGC AGAAGTGCAG AACGTCAAAC ATAAAACTAG ACAAGATAAT GAAAGAATTG
    GACGAAGAGG GGAATCAAAG AAGAAATCTA GAATCTACAG TATCTCAGAT TGAGAAGGAG
    AAAATGTTGC TACAGCACAG AATTAATGAA TACCAAAGAA AAGCAGAACA GGAAAATGAG
    AAGAGGAGGA ATGTAGAAAA TGAAGTTTCT ACATTAAAGG ATCAGTTGGA AGACTTAAAG
    AAAGTCAGCC AGAATTCACA GCTTGCTAAT GAGAAGCTGG CACAATTACA AAAGCAGCTA
    GAAGAAGCCA ATGACTTACT TAGGACAGAA TCAGACACAG CGGTTAGATT AAGGAAGAGT
    CACACAGAGA TGAGCAAATC CATCACTCAG CTAGAGTCCC TAAACAGAGA ACTGCAGGAG
    AGAAATAGAA TTTTAGAGAA TTCCAAGTCA CAAATGGACA AAGATTATTA CCAACTACAG
    GCTGTATTAG AAGCTGAACG AAGAGACAGA GGTCATGATT CTGAGAAGAT TGGAGACCTA
    CAAGCTCGAA TTACATCTTT ACAAGAGGAG GTGAAGCATC TCAAACATAA TCTTGAAAGA
    GTGGAAGGAG AAAGAAAAGA GGCTCAAGAC ATGCTTAATC ATTCAGAAAA GGAAAAGAAT
    AACTTAGAAA TAGATTTAAA CTACAAGCTT AAATCATTAC AGCAACGATT AGAACAAGAG
    GTAAATGAAC ATAAAGTAAC CAAAGCTCGT TTAACTGACA AACATCAATC TATTGAAGAG
    GCAAAGTCTG TTGCAATGTG TGAGATGGAA AAAAAGCTGA AGGAGGAGAG AGACGCTCGA
    GAGAAGGCTG AAAATAGGGT TGTTCAGATT GAGAAGCAGT GCTCTATGCT AGATGTTGAT
    CTGAAGCAAT CTCAGCAGAA GCTGGAGCAT TTGATTGAAA ATAAAGACAG GATGGAGGAT
    GAAGTTAAGA ATCTAACGCT GCAGCTGGAG CAGGAATCAA ACAAGCGACT TTTGTTACAA
    AATGAATTGA AGACTCAAGC ATTTGAGGCA GACAATTTAA AAGGTTTAGA AAAGCAGATG
    AAACAGGAAA TAAATACTTT ATTGGAAGCA AAGAGATTGT TGGAATTTGA ATTAGCTCAG
    CTTACAAAAC AGTACAGAGG GAATGAAGGG CAGATGCGGG AGCTGCAGGA TCAGCTGGAA
    GCGGAGCAGT ATTTCTCGAC ACTTTATAAA ACCCAGGTAA AGGAACTTAA GGAAGAAATT
    GAAGAAAAAA ACAGAGAAAA CTTAAAGAAA ATACAGGAAC TACAAAGTGA AAAAGAAACT
    CTTGCTACTC AGTTGGATCT AGCAGAAACA AAGGCTGAAT CTGAGCAGTT GGCTCGAGGG
    CTTCTAGAAG AACAGTATTT TGAACTGACC CAAGAAAGCA AGAAAGCTGC CTCAAGAAAT
    AGACAAGAGA TTACAGATAA AGATCACACT CTTAGTCGGC TTGAAGAAAC GAATAGCATG
    CTAACCAAAG ATATTGAATT ATTAAGAAAA GAAAACGAAG AGCTAACTGA TAAAATGAGG
    AAGGCAGAGG AAGAATATAA ATTGAAGAAG GAGGAGGAGA TCAATATCCT TAAGGCTGCC
    TTTGAAAAGA ATATCAATAC AGAACGAACC CTTAAAACAC AGGCTGTTAA CAAGTTGGCA
    GAAATAATGA ATCGAAAGGA CTTTAAAATT GATAGAAAGA AAGCAAATAC GCAGGATTTG
    AGAAAGAAAG AAAAAGAAAA TCGAAAGCTC CAACTTGAAC TCAACCAGGA AAGAGAGAAA
    TTCAACCAAA TGGTAGTGAA ACATCAGAAG GAGCTGAATG AAATGCAAGC GCAATTGGTA
    GAAGAATGTA CACATAAGAA TGAGCTTCAG ATGCAGCTGG CCAGCAAGGA GAGTGACATT
    GAGCAGCTGC GTGCCAAACT GTTGGACCTT TCAGATTCTA CAAGTGTTGC TAGTTTTCCC
    AGTGCTGATG AAACTGATGG AAACCTTCCA GAATCAAGAA TTGAAGGTTG GCTTTCAATA
    CCAAATAGAG GAAATATCAA AAGATATGGC TGGAAGAAAC AGTATGTTGT GGTAAGCAGC
    AAAAAAATTT TGTTCTATAA TGATGAACAA GATAAGGAAC AATCCAATCC GTCTATGGTA
    TTGGACATTG ATAAATTGTT TCATGTTCGT CCTGTAACAC AAGGAGATGT ATACAGAGCT
    GAAACTGAAG AAATTCCTAA AATATTCCAG ATACTGTATG CAAATGAAGG TGAATGTAGA
    AAAGACTTAG AGGTGGAACC AGTACAACAA GCTGAAAAAA CAAATTTTCA AAACCACAAA
    GGCCATGAGT TCATTCCTAC ACTCTACCAC TTTCCCGCCA ACTGTGAGGC CTGTGCCAAA
    CCTCTCTGGC ATGTTTTTAA GCCACCCCCT GCCCTGGAGT GTCGAAGATG CCATGTTAAG
    TGCCACAGAG ACCACATAGA TAAGAAAGAG GACTTGATTT CTCCATGTAA AGTAAGTTAT
    GATGTAACAT CAGCAAGAGA TATGCTGCTG CTAGCGTGTT CTCAGGATGA ACAAAAAAAA
    TGGGTAACTC ATTTAGTAAA GAAGATCCCT AAGAATCCAC CATCTGGTTT TGTGCGTGCA
    TCCCCTCGAA CACTTTCTAC AAGGTCCACT GCAAATCAGT CTTTCCGGAA AGTGGTCAAA
    AATACATCTG GAAAAGCTAG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.