TAF1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_002700041.4	XP_002700087.2	transcription initiation factor TFIID subunit 1 isoform X3
XM_015470426.1	XP_015325912.1	transcription initiation factor TFIID subunit 1 isoform X4
XM_015470427.1	XP_015325913.1	transcription initiation factor TFIID subunit 1 isoform X5
XM_005200739.3	XP_005200796.1	transcription initiation factor TFIID subunit 1 isoform X1
XM_005228045.4	XP_005228102.1	transcription initiation factor TFIID subunit 1 isoform X1
XM_005228050.4	XP_005228107.1	transcription initiation factor TFIID subunit 1 isoform X7
XM_015461657.1	XP_015317143.1	transcription initiation factor TFIID subunit 1 isoform X5
XM_005228050.3	XP_005228107.1	transcription initiation factor TFIID subunit 1 isoform X6
XM_005200740.3	XP_005200797.1	transcription initiation factor TFIID subunit 1 isoform X2
XM_002700041.5	XP_002700087.2	transcription initiation factor TFIID subunit 1 isoform X4
XM_005228052.4	XP_005228109.1	transcription initiation factor TFIID subunit 1 isoform X9
XM_005228052.3	XP_005228109.1	transcription initiation factor TFIID subunit 1 isoform X8
XM_005200742.3	XP_005200799.1	transcription initiation factor TFIID subunit 1 isoform X3
XM_005200744.3	XP_005200801.1	transcription initiation factor TFIID subunit 1 isoform X6
XM_005200745.3	XP_005200802.1	transcription initiation factor TFIID subunit 1 isoform X7
XM_005228046.4	XP_005228103.1	transcription initiation factor TFIID subunit 1 isoform X3
XM_005228045.3	XP_005228102.1	transcription initiation factor TFIID subunit 1 isoform X1
XM_005228051.3	XP_005228108.1	transcription initiation factor TFIID subunit 1 isoform X7
XM_024988721.1	XP_024844489.1	transcription initiation factor TFIID subunit 1 isoform X2
XM_024988722.1	XP_024844490.1	transcription initiation factor TFIID subunit 1 isoform X5
XM_005228048.4	XP_005228105.1	transcription initiation factor TFIID subunit 1 isoform X6
XM_005200746.3	XP_005200803.1	transcription initiation factor TFIID subunit 1 isoform X8
XM_005228046.3	XP_005228103.1	transcription initiation factor TFIID subunit 1 isoform X2
XM_005228048.3	XP_005228105.1	transcription initiation factor TFIID subunit 1 isoform X4
XM_005228051.4	XP_005228108.1	transcription initiation factor TFIID subunit 1 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa238749	XM_024988721.1 Latest version!	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa238750	XM_024988722.1 Latest version!	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa89363	XM_015461657.1 Latest version!	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa89363	XM_015470427.1 Latest version!	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa13030	XM_005228045.4 Latest version!	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa13030	XM_005200739.3 Latest version!	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa13031	XM_005200740.3 Latest version!	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa13031	XM_005228046.4 Latest version!	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa13033	XM_005200742.3 Latest version!	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa13033	XM_002700041.5 Latest version!	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa13035	XM_005200744.3 Latest version!	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa13035	XM_005228050.4 Latest version!	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa13036	XM_005200745.3 Latest version!	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa13036	XM_005228051.4 Latest version!	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa13037	XM_005228052.4 Latest version!	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa13037	XM_005200746.3 Latest version!	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa35503	XM_015470426.1 Latest version!	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa35503	XM_005228048.4 Latest version!	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OBa13035	XM_005228050.3	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa13033	XM_002700041.4	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa13037	XM_005228052.3	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa13030	XM_005228045.3	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa13031	XM_005228046.3	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa35503	XM_005228048.3	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa13036	XM_005228051.3	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

TAF1 ( XM_024988721.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844489.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa238749
Clone ID Related Accession (Same CDS sequence)	XM_024988721.1
Accession Version	XM_024988721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GGCTCAGCAG CATCAGGAAA CAATCACAGA GATGATGACA CTGCTTCTGT GACAAGCCTT 
AATTCTTCTG CCACTGGCCG TTGTCTCAAG ATTTATCGCA CGTTTCGAGA TGAGGAGGGG 
AAAGAATATG TTCGCTGTGA GACGGTCCGA AAGCCAGCTG TCATTGATGC CTATGTACGC 
ATACGGACCA CAAAAGACGA GGAATTCATT CGAAAATTTG CCCTTTTTGA TGAACAACAT 
CGAGAAGAGA TGCGAAAAGA ACGGCGGAGG ATTCAAGAGC AACTGAGACG ACTTAAGCGA 
AACCAGGAAA AGGAGAAGCT TAAGGGTCCT CCTGAGAAGA AGCCCAAAAA AATGAAGGAG 
CGTCCTGACC TAAAACTGAA ATGTGGGGCT TGTGGTGCCA TTGGGCACAT GAGAACAAAC 
AAATTCTGCC CTCTTTATTA TCAAACAAAT GCTCCCCCTT CCAACCCTGT TGCCATGACA 
GAGGAGCAGG AGGAGGAGTT GGAAAAGACA GTCATTCATA ATGATAATGA GGAACTTATC 
AAGGTTGAAG GGACCAAGAT TGTCCTAGGG AAACAGCTAA TTGAGAGTGC AGATGAGGTT 
CGCAGAAAAT CTCTGGTTCT CAAATTCCCT AAGCAGCAAC TTCCTCCCAA GAAGAAACGG 
CGAGTTGGAA CTACTGTTCA CTGTGACTAT CTGAATAGAC CTCATAAGTC CATTCACCGG 
CGCCGGACAG ACCCCATGGT GACATTGTCA TCCATTTTGG AGTCTATCAT CAATGACATG 
AGAGATCTTC CGAATACATA CCCTTTCCAC ACTCCAGTCA ATGCAAAGGT CGTAAAGGAC 
TACTACAAGA TCATCACCCG GCCAATGGAC TTACAGACAC TCCGTGAAAA TGTGCGTAAA 
CGCCTCTACC CATCTCGGGA AGAGTTCAGG GAGCATTTGG AGCTAATTGT GAAAAATAGT 
GCAACCTACA ATGGGCCAAA GCACTCACTG ACCCAGATCT CTCAATCAAT GCTGGATCTC 
TGTGATGAAA AACTTAAAGA GAAAGAAGAT AAATTGGCTC GTTTAGAGAA AGCAATCAAC 
CCCTTGCTGG ATGATGATGA CCAAGTGGCA TTTTCTTTTA TTCTGGACAA CATTGTCACC 
CAGAAAATGA TGGCAGTTCC AGATTCTTGG CCGTTTCATC ACCCAGTTAA TAAGAAGTTT 
GTTCCAGATT ATTACAAAGT AATTGTCAGT CCAATGGATT TAGAGACCAT ACGTAAAAAT 
ATCTCCAAGC ACAAGTATCA GAGTCGGGAG AGCTTTCTAG ACGATGTCAA CCTTATTCTG 
GCCAACAGTG TTAAATACAA TGGGCCTGAA AGTCAGTATA CTAAGACTGC TCAGGAGATT 
GTGAACGTCT GTTACCAGAC ACTGACTGAA TATGATGAGC ATTTAACTCA ACTTGAGAAG 
GATATTTGTA CAGCTAAAGA AGCAGCTTTG GAGGAAGCAG AATTAGAAAG CTTGGACCCA 
ATGACCCCAG GGCCTTACAC ACCTCAGCCT CCTGATTTGT ATGATACCAA CACATCCCTC 
AGTATGTCTC GAGATGCTTC TGTATTTCAA GATGAGAGCA ATATGTCTGT CCTGGATATT 
CCCACTGCCA CTCCAGAAAA GCAGGTGACA CAGATGCACC AGGGCCGAGG TAGGCTGGGC 
GAGGAAGACT CTGATGTAGA TATTGAAGGG TATGATGATG AGGAGGAGGA TGGGAAACCT 
AAGACACCAG CCCCAGAAGG TGAAGATGCA GATGGTGATC TTGCAGATGA AGAGGAAGGA 
ACTGTGCAAC AGCCTCAAGC CAGTGTCCTG TATGAGGATT TGCTTATGTC TGAAGGAGAA 
GATGATGAGG AAGATGCTGG GAGTGATGAA GAAGGAGATA ATCCTTTCTC TGCTATCCAG 
CTGAGTGAAA GTGGAAGTGA CTCGGATGTA GGATCTGGTG GGATAAGACC CAAACAGCCC 
CGCATGCTTC AAGAAAACAC AAGGATGGGC ATGGAAAATG AAGAAAGCAT GATGTCCTAT 
GAGGGAGACG GTGGGGAGGC TTCTCATGGT TTGGAGGATA GCAATATCAG TTATGGGAGC 
TATGAGGAGC CTGATCCCAA GTCGAACACC CAAGACACAA GCTTCAGCAG CATCGGTGGG 
TATGAGGTAT CAGAGGAGGA AGAAGATGAG GAGGAAGAGC AACGCTCTGG GCCGAGCGTA 
CTAAGCCAGG TCCAGCTGTC AGAGGACGAG GAGGACAGTG AGGATTTCCA CTCCATTGCT 
GGGGACAGTG ACTTGGACTC TGATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844489.1
CDS	99..5828
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQMHQGRGRLGEEDSDVDIEGYDDEEEDGKPKTPAPEGEDADGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVQLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_024988721.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988721.1

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GGCTCAGCAG CATCAGGAAA CAATCACAGA GATGATGACA CTGCTTCTGT GACAAGCCTT 
AATTCTTCTG CCACTGGCCG TTGTCTCAAG ATTTATCGCA CGTTTCGAGA TGAGGAGGGG 
AAAGAATATG TTCGCTGTGA GACGGTCCGA AAGCCAGCTG TCATTGATGC CTATGTACGC 
ATACGGACCA CAAAAGACGA GGAATTCATT CGAAAATTTG CCCTTTTTGA TGAACAACAT 
CGAGAAGAGA TGCGAAAAGA ACGGCGGAGG ATTCAAGAGC AACTGAGACG ACTTAAGCGA 
AACCAGGAAA AGGAGAAGCT TAAGGGTCCT CCTGAGAAGA AGCCCAAAAA AATGAAGGAG 
CGTCCTGACC TAAAACTGAA ATGTGGGGCT TGTGGTGCCA TTGGGCACAT GAGAACAAAC 
AAATTCTGCC CTCTTTATTA TCAAACAAAT GCTCCCCCTT CCAACCCTGT TGCCATGACA 
GAGGAGCAGG AGGAGGAGTT GGAAAAGACA GTCATTCATA ATGATAATGA GGAACTTATC 
AAGGTTGAAG GGACCAAGAT TGTCCTAGGG AAACAGCTAA TTGAGAGTGC AGATGAGGTT 
CGCAGAAAAT CTCTGGTTCT CAAATTCCCT AAGCAGCAAC TTCCTCCCAA GAAGAAACGG 
CGAGTTGGAA CTACTGTTCA CTGTGACTAT CTGAATAGAC CTCATAAGTC CATTCACCGG 
CGCCGGACAG ACCCCATGGT GACATTGTCA TCCATTTTGG AGTCTATCAT CAATGACATG 
AGAGATCTTC CGAATACATA CCCTTTCCAC ACTCCAGTCA ATGCAAAGGT CGTAAAGGAC 
TACTACAAGA TCATCACCCG GCCAATGGAC TTACAGACAC TCCGTGAAAA TGTGCGTAAA 
CGCCTCTACC CATCTCGGGA AGAGTTCAGG GAGCATTTGG AGCTAATTGT GAAAAATAGT 
GCAACCTACA ATGGGCCAAA GCACTCACTG ACCCAGATCT CTCAATCAAT GCTGGATCTC 
TGTGATGAAA AACTTAAAGA GAAAGAAGAT AAATTGGCTC GTTTAGAGAA AGCAATCAAC 
CCCTTGCTGG ATGATGATGA CCAAGTGGCA TTTTCTTTTA TTCTGGACAA CATTGTCACC 
CAGAAAATGA TGGCAGTTCC AGATTCTTGG CCGTTTCATC ACCCAGTTAA TAAGAAGTTT 
GTTCCAGATT ATTACAAAGT AATTGTCAGT CCAATGGATT TAGAGACCAT ACGTAAAAAT 
ATCTCCAAGC ACAAGTATCA GAGTCGGGAG AGCTTTCTAG ACGATGTCAA CCTTATTCTG 
GCCAACAGTG TTAAATACAA TGGGCCTGAA AGTCAGTATA CTAAGACTGC TCAGGAGATT 
GTGAACGTCT GTTACCAGAC ACTGACTGAA TATGATGAGC ATTTAACTCA ACTTGAGAAG 
GATATTTGTA CAGCTAAAGA AGCAGCTTTG GAGGAAGCAG AATTAGAAAG CTTGGACCCA 
ATGACCCCAG GGCCTTACAC ACCTCAGCCT CCTGATTTGT ATGATACCAA CACATCCCTC 
AGTATGTCTC GAGATGCTTC TGTATTTCAA GATGAGAGCA ATATGTCTGT CCTGGATATT 
CCCACTGCCA CTCCAGAAAA GCAGGTGACA CAGATGCACC AGGGCCGAGG TAGGCTGGGC 
GAGGAAGACT CTGATGTAGA TATTGAAGGG TATGATGATG AGGAGGAGGA TGGGAAACCT 
AAGACACCAG CCCCAGAAGG TGAAGATGCA GATGGTGATC TTGCAGATGA AGAGGAAGGA 
ACTGTGCAAC AGCCTCAAGC CAGTGTCCTG TATGAGGATT TGCTTATGTC TGAAGGAGAA 
GATGATGAGG AAGATGCTGG GAGTGATGAA GAAGGAGATA ATCCTTTCTC TGCTATCCAG 
CTGAGTGAAA GTGGAAGTGA CTCGGATGTA GGATCTGGTG GGATAAGACC CAAACAGCCC 
CGCATGCTTC AAGAAAACAC AAGGATGGGC ATGGAAAATG AAGAAAGCAT GATGTCCTAT 
GAGGGAGACG GTGGGGAGGC TTCTCATGGT TTGGAGGATA GCAATATCAG TTATGGGAGC 
TATGAGGAGC CTGATCCCAA GTCGAACACC CAAGACACAA GCTTCAGCAG CATCGGTGGG 
TATGAGGTAT CAGAGGAGGA AGAAGATGAG GAGGAAGAGC AACGCTCTGG GCCGAGCGTA 
CTAAGCCAGG TCCAGCTGTC AGAGGACGAG GAGGACAGTG AGGATTTCCA CTCCATTGCT 
GGGGACAGTG ACTTGGACTC TGATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_024988722.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844490.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X5, mRNA.

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CloneID	OBa238750
Clone ID Related Accession (Same CDS sequence)	XM_024988722.1
Accession Version	XM_024988722.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GGCTCAGCAG CATCAGGAAA CAATCACAGA GATGATGACA CTGCTTCTGT GACAAGCCTT 
AATTCTTCTG CCACTGGCCG TTGTCTCAAG ATTTATCGCA CGTTTCGAGA TGAGGAGGGG 
AAAGAATATG TTCGCTGTGA GACGGTCCGA AAGCCAGCTG TCATTGATGC CTATGTACGC 
ATACGGACCA CAAAAGACGA GGAATTCATT CGAAAATTTG CCCTTTTTGA TGAACAACAT 
CGAGAAGAGA TGCGAAAAGA ACGGCGGAGG ATTCAAGAGC AACTGAGACG ACTTAAGCGA 
AACCAGGAAA AGGAGAAGCT TAAGGGTCCT CCTGAGAAGA AGCCCAAAAA AATGAAGGAG 
CGTCCTGACC TAAAACTGAA ATGTGGGGCT TGTGGTGCCA TTGGGCACAT GAGAACAAAC 
AAATTCTGCC CTCTTTATTA TCAAACAAAT GCTCCCCCTT CCAACCCTGT TGCCATGACA 
GAGGAGCAGG AGGAGGAGTT GGAAAAGACA GTCATTCATA ATGATAATGA GGAACTTATC 
AAGGTTGAAG GGACCAAGAT TGTCCTAGGG AAACAGCTAA TTGAGAGTGC AGATGAGGTT 
CGCAGAAAAT CTCTGGTTCT CAAATTCCCT AAGCAGCAAC TTCCTCCCAA GAAGAAACGG 
CGAGTTGGAA CTACTGTTCA CTGTGACTAT CTGAATAGAC CTCATAAGTC CATTCACCGG 
CGCCGGACAG ACCCCATGGT GACATTGTCA TCCATTTTGG AGTCTATCAT CAATGACATG 
AGAGATCTTC CGAATACATA CCCTTTCCAC ACTCCAGTCA ATGCAAAGGT CGTAAAGGAC 
TACTACAAGA TCATCACCCG GCCAATGGAC TTACAGACAC TCCGTGAAAA TGTGCGTAAA 
CGCCTCTACC CATCTCGGGA AGAGTTCAGG GAGCATTTGG AGCTAATTGT GAAAAATAGT 
GCAACCTACA ATGGGCCAAA GCACTCACTG ACCCAGATCT CTCAATCAAT GCTGGATCTC 
TGTGATGAAA AACTTAAAGA GAAAGAAGAT AAATTGGCTC GTTTAGAGAA AGCAATCAAC 
CCCTTGCTGG ATGATGATGA CCAAGTGGCA TTTTCTTTTA TTCTGGACAA CATTGTCACC 
CAGAAAATGA TGGCAGTTCC AGATTCTTGG CCGTTTCATC ACCCAGTTAA TAAGAAGTTT 
GTTCCAGATT ATTACAAAGT AATTGTCAGT CCAATGGATT TAGAGACCAT ACGTAAAAAT 
ATCTCCAAGC ACAAGTATCA GAGTCGGGAG AGCTTTCTAG ACGATGTCAA CCTTATTCTG 
GCCAACAGTG TTAAATACAA TGGGCCTGAA AGTCAGTATA CTAAGACTGC TCAGGAGATT 
GTGAACGTCT GTTACCAGAC ACTGACTGAA TATGATGAGC ATTTAACTCA ACTTGAGAAG 
GATATTTGTA CAGCTAAAGA AGCAGCTTTG GAGGAAGCAG AATTAGAAAG CTTGGACCCA 
ATGACCCCAG GGCCTTACAC ACCTCAGCCT CCTGATTTGT ATGATACCAA CACATCCCTC 
AGTATGTCTC GAGATGCTTC TGTATTTCAA GATGAGAGCA ATATGTCTGT CCTGGATATT 
CCCACTGCCA CTCCAGAAAA GCAGGTGACA CAGGAAGGTG AAGATGCAGA TGGTGATCTT 
GCAGATGAAG AGGAAGGAAC TGTGCAACAG CCTCAAGCCA GTGTCCTGTA TGAGGATTTG 
CTTATGTCTG AAGGAGAAGA TGATGAGGAA GATGCTGGGA GTGATGAAGA AGGAGATAAT 
CCTTTCTCTG CTATCCAGCT GAGTGAAAGT GGAAGTGACT CGGATGTAGG ATCTGGTGGG 
ATAAGACCCA AACAGCCCCG CATGCTTCAA GAAAACACAA GGATGGGCAT GGAAAATGAA 
GAAAGCATGA TGTCCTATGA GGGAGACGGT GGGGAGGCTT CTCATGGTTT GGAGGATAGC 
AATATCAGTT ATGGGAGCTA TGAGGAGCCT GATCCCAAGT CGAACACCCA AGACACAAGC 
TTCAGCAGCA TCGGTGGGTA TGAGGTATCA GAGGAGGAAG AAGATGAGGA GGAAGAGCAA 
CGCTCTGGGC CGAGCGTACT AAGCCAGGTC CAGCTGTCAG AGGACGAGGA GGACAGTGAG 
GATTTCCACT CCATTGCTGG GGACAGTGAC TTGGACTCTG ATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844490.1
CDS	7..5634
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQEGEDADGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVQLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_024988722.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988722.1

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GGCTCAGCAG CATCAGGAAA CAATCACAGA GATGATGACA CTGCTTCTGT GACAAGCCTT 
AATTCTTCTG CCACTGGCCG TTGTCTCAAG ATTTATCGCA CGTTTCGAGA TGAGGAGGGG 
AAAGAATATG TTCGCTGTGA GACGGTCCGA AAGCCAGCTG TCATTGATGC CTATGTACGC 
ATACGGACCA CAAAAGACGA GGAATTCATT CGAAAATTTG CCCTTTTTGA TGAACAACAT 
CGAGAAGAGA TGCGAAAAGA ACGGCGGAGG ATTCAAGAGC AACTGAGACG ACTTAAGCGA 
AACCAGGAAA AGGAGAAGCT TAAGGGTCCT CCTGAGAAGA AGCCCAAAAA AATGAAGGAG 
CGTCCTGACC TAAAACTGAA ATGTGGGGCT TGTGGTGCCA TTGGGCACAT GAGAACAAAC 
AAATTCTGCC CTCTTTATTA TCAAACAAAT GCTCCCCCTT CCAACCCTGT TGCCATGACA 
GAGGAGCAGG AGGAGGAGTT GGAAAAGACA GTCATTCATA ATGATAATGA GGAACTTATC 
AAGGTTGAAG GGACCAAGAT TGTCCTAGGG AAACAGCTAA TTGAGAGTGC AGATGAGGTT 
CGCAGAAAAT CTCTGGTTCT CAAATTCCCT AAGCAGCAAC TTCCTCCCAA GAAGAAACGG 
CGAGTTGGAA CTACTGTTCA CTGTGACTAT CTGAATAGAC CTCATAAGTC CATTCACCGG 
CGCCGGACAG ACCCCATGGT GACATTGTCA TCCATTTTGG AGTCTATCAT CAATGACATG 
AGAGATCTTC CGAATACATA CCCTTTCCAC ACTCCAGTCA ATGCAAAGGT CGTAAAGGAC 
TACTACAAGA TCATCACCCG GCCAATGGAC TTACAGACAC TCCGTGAAAA TGTGCGTAAA 
CGCCTCTACC CATCTCGGGA AGAGTTCAGG GAGCATTTGG AGCTAATTGT GAAAAATAGT 
GCAACCTACA ATGGGCCAAA GCACTCACTG ACCCAGATCT CTCAATCAAT GCTGGATCTC 
TGTGATGAAA AACTTAAAGA GAAAGAAGAT AAATTGGCTC GTTTAGAGAA AGCAATCAAC 
CCCTTGCTGG ATGATGATGA CCAAGTGGCA TTTTCTTTTA TTCTGGACAA CATTGTCACC 
CAGAAAATGA TGGCAGTTCC AGATTCTTGG CCGTTTCATC ACCCAGTTAA TAAGAAGTTT 
GTTCCAGATT ATTACAAAGT AATTGTCAGT CCAATGGATT TAGAGACCAT ACGTAAAAAT 
ATCTCCAAGC ACAAGTATCA GAGTCGGGAG AGCTTTCTAG ACGATGTCAA CCTTATTCTG 
GCCAACAGTG TTAAATACAA TGGGCCTGAA AGTCAGTATA CTAAGACTGC TCAGGAGATT 
GTGAACGTCT GTTACCAGAC ACTGACTGAA TATGATGAGC ATTTAACTCA ACTTGAGAAG 
GATATTTGTA CAGCTAAAGA AGCAGCTTTG GAGGAAGCAG AATTAGAAAG CTTGGACCCA 
ATGACCCCAG GGCCTTACAC ACCTCAGCCT CCTGATTTGT ATGATACCAA CACATCCCTC 
AGTATGTCTC GAGATGCTTC TGTATTTCAA GATGAGAGCA ATATGTCTGT CCTGGATATT 
CCCACTGCCA CTCCAGAAAA GCAGGTGACA CAGGAAGGTG AAGATGCAGA TGGTGATCTT 
GCAGATGAAG AGGAAGGAAC TGTGCAACAG CCTCAAGCCA GTGTCCTGTA TGAGGATTTG 
CTTATGTCTG AAGGAGAAGA TGATGAGGAA GATGCTGGGA GTGATGAAGA AGGAGATAAT 
CCTTTCTCTG CTATCCAGCT GAGTGAAAGT GGAAGTGACT CGGATGTAGG ATCTGGTGGG 
ATAAGACCCA AACAGCCCCG CATGCTTCAA GAAAACACAA GGATGGGCAT GGAAAATGAA 
GAAAGCATGA TGTCCTATGA GGGAGACGGT GGGGAGGCTT CTCATGGTTT GGAGGATAGC 
AATATCAGTT ATGGGAGCTA TGAGGAGCCT GATCCCAAGT CGAACACCCA AGACACAAGC 
TTCAGCAGCA TCGGTGGGTA TGAGGTATCA GAGGAGGAAG AAGATGAGGA GGAAGAGCAA 
CGCTCTGGGC CGAGCGTACT AAGCCAGGTC CAGCTGTCAG AGGACGAGGA GGACAGTGAG 
GATTTCCACT CCATTGCTGG GGACAGTGAC TTGGACTCTG ATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_015470427.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325913.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X5, mRNA.

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CloneID	OBa89363
Clone ID Related Accession (Same CDS sequence)	XM_015470427.1 , XM_015461657.1
Accession Version	XM_015470427.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647142.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG GCTAAGGTGA GTGAGGGCCA TGGGTCTTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325913.1
CDS	106..5088
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQAKVSEGHGSW

Target ORF information:

RefSeq Version	XM_015470427.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470427.1

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG GCTAAGGTGA GTGAGGGCCA TGGGTCTTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_015461657.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015317143.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa89363
Clone ID Related Accession (Same CDS sequence)	XM_015470427.1 , XM_015461657.1
Accession Version	XM_015461657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104728.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG GCTAAGGTGA GTGAGGGCCA TGGGTCTTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015317143.1
CDS	106..5088
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQAKVSEGHGSW

Target ORF information:

RefSeq Version	XM_015461657.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461657.1

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG GCTAAGGTGA GTGAGGGCCA TGGGTCTTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005200739.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005200796.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa13030
Clone ID Related Accession (Same CDS sequence)	XM_005200739.3 , XM_005228045.4 , XM_005228045.3
Accession Version	XM_005200739.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647142.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005200739.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG CCTCCTGATT TGTATGATAC CAACACATCC 
CTCAGTATGT CTCGAGATGC TTCTGTATTT CAAGATGAGA GCAATATGTC TGTCCTGGAT 
ATTCCCACTG CCACTCCAGA AAAGCAGGTG ACACAGATGC ACCAGGGCCG AGGTAGGCTG 
GGCGAGGAAG ACTCTGATGT AGATATTGAA GGGTATGATG ATGAGGAGGA GGATGGGAAA 
CCTAAGACAC CAGCCCCAGA AGGTGAAGAT GCAGATGGTG ATCTTGCAGA TGAAGAGGAA 
GGAACTGTGC AACAGCCTCA AGCCAGTGTC CTGTATGAGG ATTTGCTTAT GTCTGAAGGA 
GAAGATGATG AGGAAGATGC TGGGAGTGAT GAAGAAGGAG ATAATCCTTT CTCTGCTATC 
CAGCTGAGTG AAAGTGGAAG TGACTCGGAT GTAGGATCTG GTGGGATAAG ACCCAAACAG 
CCCCGCATGC TTCAAGAAAA CACAAGGATG GGCATGGAAA ATGAAGAAAG CATGATGTCC 
TATGAGGGAG ACGGTGGGGA GGCTTCTCAT GGTTTGGAGG ATAGCAATAT CAGTTATGGG 
AGCTATGAGG AGCCTGATCC CAAGTCGAAC ACCCAAGACA CAAGCTTCAG CAGCATCGGT 
GGGTATGAGG TATCAGAGGA GGAAGAAGAT GAGGAGGAAG AGCAACGCTC TGGGCCGAGC 
GTACTAAGCC AGGTCCAGCT GTCAGAGGAC GAGGAGGACA GTGAGGATTT CCACTCCATT 
GCTGGGGACA GTGACTTGGA CTCTGATGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005200796.1
CDS	106..5838
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQMHQGRGRLGEEDSDVDIEGYDDEEEDGKPKTPAPEGEDADGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVQLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_005200739.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005200739.3

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG CCTCCTGATT TGTATGATAC CAACACATCC 
CTCAGTATGT CTCGAGATGC TTCTGTATTT CAAGATGAGA GCAATATGTC TGTCCTGGAT 
ATTCCCACTG CCACTCCAGA AAAGCAGGTG ACACAGATGC ACCAGGGCCG AGGTAGGCTG 
GGCGAGGAAG ACTCTGATGT AGATATTGAA GGGTATGATG ATGAGGAGGA GGATGGGAAA 
CCTAAGACAC CAGCCCCAGA AGGTGAAGAT GCAGATGGTG ATCTTGCAGA TGAAGAGGAA 
GGAACTGTGC AACAGCCTCA AGCCAGTGTC CTGTATGAGG ATTTGCTTAT GTCTGAAGGA 
GAAGATGATG AGGAAGATGC TGGGAGTGAT GAAGAAGGAG ATAATCCTTT CTCTGCTATC 
CAGCTGAGTG AAAGTGGAAG TGACTCGGAT GTAGGATCTG GTGGGATAAG ACCCAAACAG 
CCCCGCATGC TTCAAGAAAA CACAAGGATG GGCATGGAAA ATGAAGAAAG CATGATGTCC 
TATGAGGGAG ACGGTGGGGA GGCTTCTCAT GGTTTGGAGG ATAGCAATAT CAGTTATGGG 
AGCTATGAGG AGCCTGATCC CAAGTCGAAC ACCCAAGACA CAAGCTTCAG CAGCATCGGT 
GGGTATGAGG TATCAGAGGA GGAAGAAGAT GAGGAGGAAG AGCAACGCTC TGGGCCGAGC 
GTACTAAGCC AGGTCCAGCT GTCAGAGGAC GAGGAGGACA GTGAGGATTT CCACTCCATT 
GCTGGGGACA GTGACTTGGA CTCTGATGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005228045.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228102.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X1, mRNA.

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CloneID	OBa13030
Clone ID Related Accession (Same CDS sequence)	XM_005200739.3 , XM_005228045.4 , XM_005228045.3
Accession Version	XM_005228045.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228045.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG CCTCCTGATT TGTATGATAC CAACACATCC 
CTCAGTATGT CTCGAGATGC TTCTGTATTT CAAGATGAGA GCAATATGTC TGTCCTGGAT 
ATTCCCACTG CCACTCCAGA AAAGCAGGTG ACACAGATGC ACCAGGGCCG AGGTAGGCTG 
GGCGAGGAAG ACTCTGATGT AGATATTGAA GGGTATGATG ATGAGGAGGA GGATGGGAAA 
CCTAAGACAC CAGCCCCAGA AGGTGAAGAT GCAGATGGTG ATCTTGCAGA TGAAGAGGAA 
GGAACTGTGC AACAGCCTCA AGCCAGTGTC CTGTATGAGG ATTTGCTTAT GTCTGAAGGA 
GAAGATGATG AGGAAGATGC TGGGAGTGAT GAAGAAGGAG ATAATCCTTT CTCTGCTATC 
CAGCTGAGTG AAAGTGGAAG TGACTCGGAT GTAGGATCTG GTGGGATAAG ACCCAAACAG 
CCCCGCATGC TTCAAGAAAA CACAAGGATG GGCATGGAAA ATGAAGAAAG CATGATGTCC 
TATGAGGGAG ACGGTGGGGA GGCTTCTCAT GGTTTGGAGG ATAGCAATAT CAGTTATGGG 
AGCTATGAGG AGCCTGATCC CAAGTCGAAC ACCCAAGACA CAAGCTTCAG CAGCATCGGT 
GGGTATGAGG TATCAGAGGA GGAAGAAGAT GAGGAGGAAG AGCAACGCTC TGGGCCGAGC 
GTACTAAGCC AGGTCCAGCT GTCAGAGGAC GAGGAGGACA GTGAGGATTT CCACTCCATT 
GCTGGGGACA GTGACTTGGA CTCTGATGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228102.1
CDS	99..5831
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQMHQGRGRLGEEDSDVDIEGYDDEEEDGKPKTPAPEGEDADGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVQLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_005228045.4
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228045.4

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG CCTCCTGATT TGTATGATAC CAACACATCC 
CTCAGTATGT CTCGAGATGC TTCTGTATTT CAAGATGAGA GCAATATGTC TGTCCTGGAT 
ATTCCCACTG CCACTCCAGA AAAGCAGGTG ACACAGATGC ACCAGGGCCG AGGTAGGCTG 
GGCGAGGAAG ACTCTGATGT AGATATTGAA GGGTATGATG ATGAGGAGGA GGATGGGAAA 
CCTAAGACAC CAGCCCCAGA AGGTGAAGAT GCAGATGGTG ATCTTGCAGA TGAAGAGGAA 
GGAACTGTGC AACAGCCTCA AGCCAGTGTC CTGTATGAGG ATTTGCTTAT GTCTGAAGGA 
GAAGATGATG AGGAAGATGC TGGGAGTGAT GAAGAAGGAG ATAATCCTTT CTCTGCTATC 
CAGCTGAGTG AAAGTGGAAG TGACTCGGAT GTAGGATCTG GTGGGATAAG ACCCAAACAG 
CCCCGCATGC TTCAAGAAAA CACAAGGATG GGCATGGAAA ATGAAGAAAG CATGATGTCC 
TATGAGGGAG ACGGTGGGGA GGCTTCTCAT GGTTTGGAGG ATAGCAATAT CAGTTATGGG 
AGCTATGAGG AGCCTGATCC CAAGTCGAAC ACCCAAGACA CAAGCTTCAG CAGCATCGGT 
GGGTATGAGG TATCAGAGGA GGAAGAAGAT GAGGAGGAAG AGCAACGCTC TGGGCCGAGC 
GTACTAAGCC AGGTCCAGCT GTCAGAGGAC GAGGAGGACA GTGAGGATTT CCACTCCATT 
GCTGGGGACA GTGACTTGGA CTCTGATGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005228045.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228102.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X1, mRNA.

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CloneID	OBa13030
Clone ID Related Accession (Same CDS sequence)	XM_005200739.3 , XM_005228045.4 , XM_005228045.3
Accession Version	XM_005228045.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104728.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228045.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG CCTCCTGATT TGTATGATAC CAACACATCC 
CTCAGTATGT CTCGAGATGC TTCTGTATTT CAAGATGAGA GCAATATGTC TGTCCTGGAT 
ATTCCCACTG CCACTCCAGA AAAGCAGGTG ACACAGATGC ACCAGGGCCG AGGTAGGCTG 
GGCGAGGAAG ACTCTGATGT AGATATTGAA GGGTATGATG ATGAGGAGGA GGATGGGAAA 
CCTAAGACAC CAGCCCCAGA AGGTGAAGAT GCAGATGGTG ATCTTGCAGA TGAAGAGGAA 
GGAACTGTGC AACAGCCTCA AGCCAGTGTC CTGTATGAGG ATTTGCTTAT GTCTGAAGGA 
GAAGATGATG AGGAAGATGC TGGGAGTGAT GAAGAAGGAG ATAATCCTTT CTCTGCTATC 
CAGCTGAGTG AAAGTGGAAG TGACTCGGAT GTAGGATCTG GTGGGATAAG ACCCAAACAG 
CCCCGCATGC TTCAAGAAAA CACAAGGATG GGCATGGAAA ATGAAGAAAG CATGATGTCC 
TATGAGGGAG ACGGTGGGGA GGCTTCTCAT GGTTTGGAGG ATAGCAATAT CAGTTATGGG 
AGCTATGAGG AGCCTGATCC CAAGTCGAAC ACCCAAGACA CAAGCTTCAG CAGCATCGGT 
GGGTATGAGG TATCAGAGGA GGAAGAAGAT GAGGAGGAAG AGCAACGCTC TGGGCCGAGC 
GTACTAAGCC AGGTCCAGCT GTCAGAGGAC GAGGAGGACA GTGAGGATTT CCACTCCATT 
GCTGGGGACA GTGACTTGGA CTCTGATGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228102.1
CDS	106..5838
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQMHQGRGRLGEEDSDVDIEGYDDEEEDGKPKTPAPEGEDADGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVQLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_005228045.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228045.3

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG CCTCCTGATT TGTATGATAC CAACACATCC 
CTCAGTATGT CTCGAGATGC TTCTGTATTT CAAGATGAGA GCAATATGTC TGTCCTGGAT 
ATTCCCACTG CCACTCCAGA AAAGCAGGTG ACACAGATGC ACCAGGGCCG AGGTAGGCTG 
GGCGAGGAAG ACTCTGATGT AGATATTGAA GGGTATGATG ATGAGGAGGA GGATGGGAAA 
CCTAAGACAC CAGCCCCAGA AGGTGAAGAT GCAGATGGTG ATCTTGCAGA TGAAGAGGAA 
GGAACTGTGC AACAGCCTCA AGCCAGTGTC CTGTATGAGG ATTTGCTTAT GTCTGAAGGA 
GAAGATGATG AGGAAGATGC TGGGAGTGAT GAAGAAGGAG ATAATCCTTT CTCTGCTATC 
CAGCTGAGTG AAAGTGGAAG TGACTCGGAT GTAGGATCTG GTGGGATAAG ACCCAAACAG 
CCCCGCATGC TTCAAGAAAA CACAAGGATG GGCATGGAAA ATGAAGAAAG CATGATGTCC 
TATGAGGGAG ACGGTGGGGA GGCTTCTCAT GGTTTGGAGG ATAGCAATAT CAGTTATGGG 
AGCTATGAGG AGCCTGATCC CAAGTCGAAC ACCCAAGACA CAAGCTTCAG CAGCATCGGT 
GGGTATGAGG TATCAGAGGA GGAAGAAGAT GAGGAGGAAG AGCAACGCTC TGGGCCGAGC 
GTACTAAGCC AGGTCCAGCT GTCAGAGGAC GAGGAGGACA GTGAGGATTT CCACTCCATT 
GCTGGGGACA GTGACTTGGA CTCTGATGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005200740.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005200797.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X2, mRNA.

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CloneID	OBa13031
Clone ID Related Accession (Same CDS sequence)	XM_005200740.3 , XM_005228046.4 , XM_005228046.3
Accession Version	XM_005200740.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647142.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005200740.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGAGAAAGTG 
GACTTCAGTA GTTCCTCTGA CTCAGAATCT GAGATGGGAC CTCAGGAAGC AGCACAGACA 
GAATCTGAGG ATGGAAAGCT GACCCTACCA TTGGCTGGGA TTATGCAGCA CGATGCCACC 
AAGCTGTTGC CGAGTGTCAC GGAACTTTTC CCAGAATTTC GTCCTGGGAA GGTGTTACGC 
TTCCTACGTC TTTTTGGACC AGGAAAGAAT GTCCCATCTG TTTGGCGCAG TGCTCGGAGA 
AAGAGGAAGA AGAAACACCG TGAACTGATA CAGGAAGAGC AGATCCAGGA GACGGAGTGC 
TCAGTAGAAT CAGAAGTCAA CCAGAAATCT TTGTGGAACT ATGACTATGC CCCACCACCA 
CCTCCAGAGC AGTGTCTCTC TGATGATGAA ATCACAATGA TGGCACCTGT GGAATCCAAG 
TTTTCCCAGT CAACTGGAGA TACAGATAAA GTGACAGACA CCAAACCAAG AGTGGCTGAG 
TGGCGTTATG GGCCTGCTCG ACTGTGGTAT GATATGCTGG GTGTCCCTGA AGATGGCAGT 
GGGTTTGACT ATGGCTTCAA ACTAAGAAAG ATGGAACAGG AACCTGTGAT GAAATGTAGA 
ATGGCGGATG TAAGCAACAC GGAATACAGG ATACCTGAGG AAAGCAGTGG CACCGATCTT 
CTGGCTGATG AAAACTTCCT GATGGTGACA CAGCTGCATT GGGAAGATGA TATCATCTGG 
GATGGGGAGG ATGTTAAACA CAAAGGGACA AAACCACAGC GTGCTAGCCT GGCAGGCTGG 
CTTCCTTCCA GCATGACTAG GAATGCCATG GCTTACAATG TTCAACAAGG TTTTGCAGCA 
ACGCTGGATG ATGACAAGCC TTGGTACTCC ATTTTTCCCA TTGACAATGA GGAGCTGGTA 
TATGGACGTT GGGAGGACAA TATCATTTGG GATGCTCAGG CCATGCCCCG GTTCTTGGAG 
CCTCCTATTT TGACACTTGA TCCCAATGAT GAGAACCTCA TTTTGGAAAT TCCAGATGAG 
AAGGAAGAGG CTACTTCTAA CTCTCCCTCC AAGGAGAGTA AGAAGGAATC ATCTCTGAAG 
AAGAGTCGAA TTCTGTTAGG GAAAACAGGA GTCATCAAGG AGGAACCACA GCAGAACATG 
TCTCAACCAG AAGTGAAAGA TCCATGGAAT CTCTCCAATG ATGAATATTA CTACCCTAAG 
CAACAGGGTC TTCGAGGCAC CTTTGGAGGA AATATTATTC AGCATTCAAT TCCTGCTGTG 
GAATTACGGC AGCCCTTCTT TCCTACCCAC ATGGGGCCCA TCAAACTCCG GCAGTTCCAT 
CGCCCACCTC TGAAGAAGTA CTCTTTTGGG GCACTATCTC AGCCAGGTCC CCACTCAGTC 
CAGCCCTTGC TAAAGCACAT CAAAAAGAAG GCAAAGATGA GAGAACAAGA GAGGCAAGCT 
TCAGGTGGTG GAGAGATGTT TTTCATGCGC ACACCTCAGG ACCTCACAGG CAAAGATGGA 
GATCTTATTC TTGCAGAATA TAGTGAGGAA AACGGACCAT TAATGATGCA AGTTGGCATG 
GCAACCAAGA TAAAAAACTA CTATAAGCGG AAACCCGGAA AAGATCCTGG AGCCCCAGAT 
TGTAAATATG GAGAAACTGT TTACTGCCAT ACATCTCCTT TTCTAGGCTC ACTCCATCCT 
GGACAATTGC TGCAGGCATT TGAGAACAAC CTTTTTCGTG CTCCAATTTA TCTTCATAAG 
ATGCCAGAAA CTGACTTCCT TATTATTCGC ACAAGACAAG GTTACTATAT TCGGGAATTA 
GTGGATATTT TTGTGGTTGG CCAGCAGTGC CCTTTGTTTG AAGTTCCTGG GCCCAACTCC 
AAAAGGGCCA ATACTCATAT TCGAGACTTT CTCCAGGTTT TTATTTATCG CCTGTTCTGG 
AAGAGTAAAG ATCGCCCACG ACGGATCCGA ATGGAAGATA TAAAAAAAGC CTTTCCTTCT 
CACTCAGAAA GCAGCATTCG GAAGAGGCTA AAGCTCTGCG CTGACTTCAA ACGCACAGGC 
ATGGATTCAA ACTGGTGGGT TCTGAAGTCT GATTTTCGTT TACCAACTGA AGAGGAGATC 
AGGGCTATGG TGTCTCCAGA GCAATGCTGT GCTTATTACA GCATGATAGC TGCAGAGCAG 
CGACTGAAGG ATGCTGGCTA TGGTGAGAAG TCCTTTTTTG CTCCAGAAGA GGAAAATGAG 
GAAGATTTCC AGATGAAGAT TGATGATGAG GTTCGCACTG CTCCTTGGAA CACCACACGG 
GCTTTCATTG CTGCCATGAA GGGCAAGTGT CTCCTGGAGG TGACTGGGGT GGCAGATCCC 
ACAGGGTGTG GTGAAGGATT CTCCTATGTG AAGATTCCAA ACAAACCAAC ACAGCAGAAG 
GATGATAAGG AGCCTCAGCC AGTGAAGAAG ACAGTGACAG GAACAGACGC AGACCTCCGT 
CGCCTTTCAC TGAAAAATGC CAAGCAACTT CTCCGTAAAT TTGGTGTGCC TGAGGAAGAG 
ATTAAGAAGT TGTCCCGCTG GGAAGTGATC GATGTAGTAC GCACAATGTC AACAGAACAA 
GCCCGCTCTG GAGAGGGACC CATGAGTAAA TTTGCCCGTG GATCAAGGTT TTCTGTGGCT 
GAGCATCAAG AGCGTTACAA AGAGGAATGT CAGCGCATCT TTGACCTGCA GAACAAGGTT 
TTATCATCAA CTGAAATCTT ATCAACTGAC ACAGACAGCA GCTCAGCTGA AGACAGTGAC 
TTTGAGGAAA TGGGAAAAAA CATAGAGAAC ATGTTGCAGA ATAAGAAAAC CAGCTCTCAG 
CTGTCACGTG AACGGGAGGA GCAGGAACGG AAGGAACTAC AGAGGATGAT ACTGGCAGCA 
GGCTCAGCAG CATCAGGAAA CAATCACAGA GATGATGACA CTGCTTCTGT GACAAGCCTT 
AATTCTTCTG CCACTGGCCG TTGTCTCAAG ATTTATCGCA CGTTTCGAGA TGAGGAGGGG 
AAAGAATATG TTCGCTGTGA GACGGTCCGA AAGCCAGCTG TCATTGATGC CTATGTACGC 
ATACGGACCA CAAAAGACGA GGAATTCATT CGAAAATTTG CCCTTTTTGA TGAACAACAT 
CGAGAAGAGA TGCGAAAAGA ACGGCGGAGG ATTCAAGAGC AACTGAGACG ACTTAAGCGA 
AACCAGGAAA AGGAGAAGCT TAAGGGTCCT CCTGAGAAGA AGCCCAAAAA AATGAAGGAG 
CGTCCTGACC TAAAACTGAA ATGTGGGGCT TGTGGTGCCA TTGGGCACAT GAGAACAAAC 
AAATTCTGCC CTCTTTATTA TCAAACAAAT GCTCCCCCTT CCAACCCTGT TGCCATGACA 
GAGGAGCAGG AGGAGGAGTT GGAAAAGACA GTCATTCATA ATGATAATGA GGAACTTATC 
AAGGTTGAAG GGACCAAGAT TGTCCTAGGG AAACAGCTAA TTGAGAGTGC AGATGAGGTT 
CGCAGAAAAT CTCTGGTTCT CAAATTCCCT AAGCAGCAAC TTCCTCCCAA GAAGAAACGG 
CGAGTTGGAA CTACTGTTCA CTGTGACTAT CTGAATAGAC CTCATAAGTC CATTCACCGG 
CGCCGGACAG ACCCCATGGT GACATTGTCA TCCATTTTGG AGTCTATCAT CAATGACATG 
AGAGATCTTC CGAATACATA CCCTTTCCAC ACTCCAGTCA ATGCAAAGGT CGTAAAGGAC 
TACTACAAGA TCATCACCCG GCCAATGGAC TTACAGACAC TCCGTGAAAA TGTGCGTAAA 
CGCCTCTACC CATCTCGGGA AGAGTTCAGG GAGCATTTGG AGCTAATTGT GAAAAATAGT 
GCAACCTACA ATGGGCCAAA GCACTCACTG ACCCAGATCT CTCAATCAAT GCTGGATCTC 
TGTGATGAAA AACTTAAAGA GAAAGAAGAT AAATTGGCTC GTTTAGAGAA AGCAATCAAC 
CCCTTGCTGG ATGATGATGA CCAAGTGGCA TTTTCTTTTA TTCTGGACAA CATTGTCACC 
CAGAAAATGA TGGCAGTTCC AGATTCTTGG CCGTTTCATC ACCCAGTTAA TAAGAAGTTT 
GTTCCAGATT ATTACAAAGT AATTGTCAGT CCAATGGATT TAGAGACCAT ACGTAAAAAT 
ATCTCCAAGC ACAAGTATCA GAGTCGGGAG AGCTTTCTAG ACGATGTCAA CCTTATTCTG 
GCCAACAGTG TTAAATACAA TGGGCCTGAA AGTCAGTATA CTAAGACTGC TCAGGAGATT 
GTGAACGTCT GTTACCAGAC ACTGACTGAA TATGATGAGC ATTTAACTCA ACTTGAGAAG 
GATATTTGTA CAGCTAAAGA AGCAGCTTTG GAGGAAGCAG AATTAGAAAG CTTGGACCCA 
ATGACCCCAG GGCCTTACAC ACCTCAGCCT CCTGATTTGT ATGATACCAA CACATCCCTC 
AGTATGTCTC GAGATGCTTC TGTATTTCAA GATGAGAGCA ATATGTCTGT CCTGGATATT 
CCCACTGCCA CTCCAGAAAA GCAGGTGACA CAGATGCACC AGGGCCGAGG TAGGCTGGGC 
GAGGAAGACT CTGATGTAGA TATTGAAGGG TATGATGATG AGGAGGAGGA TGGGAAACCT 
AAGACACCAG CCCCAGAAGG TGAAGATGCA GATGGTGATC TTGCAGATGA AGAGGAAGGA 
ACTGTGCAAC AGCCTCAAGC CAGTGTCCTG TATGAGGATT TGCTTATGTC TGAAGGAGAA 
GATGATGAGG AAGATGCTGG GAGTGATGAA GAAGGAGATA ATCCTTTCTC TGCTATCCAG 
CTGAGTGAAA GTGGAAGTGA CTCGGATGTA GGATCTGGTG GGATAAGACC CAAACAGCCC 
CGCATGCTTC AAGAAAACAC AAGGATGGGC ATGGAAAATG AAGAAAGCAT GATGTCCTAT 
GAGGGAGACG GTGGGGAGGC TTCTCATGGT TTGGAGGATA GCAATATCAG TTATGGGAGC 
TATGAGGAGC CTGATCCCAA GTCGAACACC CAAGACACAA GCTTCAGCAG CATCGGTGGG 
TATGAGGTAT CAGAGGAGGA AGAAGATGAG GAGGAAGAGC AACGCTCTGG GCCGAGCGTA 
CTAAGCCAGG TCCAGCTGTC AGAGGACGAG GAGGACAGTG AGGATTTCCA CTCCATTGCT 
GGGGACAGTG ACTTGGACTC TGATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005200797.1
CDS	106..5775
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQMHQGRGRLGEEDSDVDIEGYDDEEEDGKPKTPAPEGEDADGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVQLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_005200740.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005200740.3

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGAGAAAGTG 
GACTTCAGTA GTTCCTCTGA CTCAGAATCT GAGATGGGAC CTCAGGAAGC AGCACAGACA 
GAATCTGAGG ATGGAAAGCT GACCCTACCA TTGGCTGGGA TTATGCAGCA CGATGCCACC 
AAGCTGTTGC CGAGTGTCAC GGAACTTTTC CCAGAATTTC GTCCTGGGAA GGTGTTACGC 
TTCCTACGTC TTTTTGGACC AGGAAAGAAT GTCCCATCTG TTTGGCGCAG TGCTCGGAGA 
AAGAGGAAGA AGAAACACCG TGAACTGATA CAGGAAGAGC AGATCCAGGA GACGGAGTGC 
TCAGTAGAAT CAGAAGTCAA CCAGAAATCT TTGTGGAACT ATGACTATGC CCCACCACCA 
CCTCCAGAGC AGTGTCTCTC TGATGATGAA ATCACAATGA TGGCACCTGT GGAATCCAAG 
TTTTCCCAGT CAACTGGAGA TACAGATAAA GTGACAGACA CCAAACCAAG AGTGGCTGAG 
TGGCGTTATG GGCCTGCTCG ACTGTGGTAT GATATGCTGG GTGTCCCTGA AGATGGCAGT 
GGGTTTGACT ATGGCTTCAA ACTAAGAAAG ATGGAACAGG AACCTGTGAT GAAATGTAGA 
ATGGCGGATG TAAGCAACAC GGAATACAGG ATACCTGAGG AAAGCAGTGG CACCGATCTT 
CTGGCTGATG AAAACTTCCT GATGGTGACA CAGCTGCATT GGGAAGATGA TATCATCTGG 
GATGGGGAGG ATGTTAAACA CAAAGGGACA AAACCACAGC GTGCTAGCCT GGCAGGCTGG 
CTTCCTTCCA GCATGACTAG GAATGCCATG GCTTACAATG TTCAACAAGG TTTTGCAGCA 
ACGCTGGATG ATGACAAGCC TTGGTACTCC ATTTTTCCCA TTGACAATGA GGAGCTGGTA 
TATGGACGTT GGGAGGACAA TATCATTTGG GATGCTCAGG CCATGCCCCG GTTCTTGGAG 
CCTCCTATTT TGACACTTGA TCCCAATGAT GAGAACCTCA TTTTGGAAAT TCCAGATGAG 
AAGGAAGAGG CTACTTCTAA CTCTCCCTCC AAGGAGAGTA AGAAGGAATC ATCTCTGAAG 
AAGAGTCGAA TTCTGTTAGG GAAAACAGGA GTCATCAAGG AGGAACCACA GCAGAACATG 
TCTCAACCAG AAGTGAAAGA TCCATGGAAT CTCTCCAATG ATGAATATTA CTACCCTAAG 
CAACAGGGTC TTCGAGGCAC CTTTGGAGGA AATATTATTC AGCATTCAAT TCCTGCTGTG 
GAATTACGGC AGCCCTTCTT TCCTACCCAC ATGGGGCCCA TCAAACTCCG GCAGTTCCAT 
CGCCCACCTC TGAAGAAGTA CTCTTTTGGG GCACTATCTC AGCCAGGTCC CCACTCAGTC 
CAGCCCTTGC TAAAGCACAT CAAAAAGAAG GCAAAGATGA GAGAACAAGA GAGGCAAGCT 
TCAGGTGGTG GAGAGATGTT TTTCATGCGC ACACCTCAGG ACCTCACAGG CAAAGATGGA 
GATCTTATTC TTGCAGAATA TAGTGAGGAA AACGGACCAT TAATGATGCA AGTTGGCATG 
GCAACCAAGA TAAAAAACTA CTATAAGCGG AAACCCGGAA AAGATCCTGG AGCCCCAGAT 
TGTAAATATG GAGAAACTGT TTACTGCCAT ACATCTCCTT TTCTAGGCTC ACTCCATCCT 
GGACAATTGC TGCAGGCATT TGAGAACAAC CTTTTTCGTG CTCCAATTTA TCTTCATAAG 
ATGCCAGAAA CTGACTTCCT TATTATTCGC ACAAGACAAG GTTACTATAT TCGGGAATTA 
GTGGATATTT TTGTGGTTGG CCAGCAGTGC CCTTTGTTTG AAGTTCCTGG GCCCAACTCC 
AAAAGGGCCA ATACTCATAT TCGAGACTTT CTCCAGGTTT TTATTTATCG CCTGTTCTGG 
AAGAGTAAAG ATCGCCCACG ACGGATCCGA ATGGAAGATA TAAAAAAAGC CTTTCCTTCT 
CACTCAGAAA GCAGCATTCG GAAGAGGCTA AAGCTCTGCG CTGACTTCAA ACGCACAGGC 
ATGGATTCAA ACTGGTGGGT TCTGAAGTCT GATTTTCGTT TACCAACTGA AGAGGAGATC 
AGGGCTATGG TGTCTCCAGA GCAATGCTGT GCTTATTACA GCATGATAGC TGCAGAGCAG 
CGACTGAAGG ATGCTGGCTA TGGTGAGAAG TCCTTTTTTG CTCCAGAAGA GGAAAATGAG 
GAAGATTTCC AGATGAAGAT TGATGATGAG GTTCGCACTG CTCCTTGGAA CACCACACGG 
GCTTTCATTG CTGCCATGAA GGGCAAGTGT CTCCTGGAGG TGACTGGGGT GGCAGATCCC 
ACAGGGTGTG GTGAAGGATT CTCCTATGTG AAGATTCCAA ACAAACCAAC ACAGCAGAAG 
GATGATAAGG AGCCTCAGCC AGTGAAGAAG ACAGTGACAG GAACAGACGC AGACCTCCGT 
CGCCTTTCAC TGAAAAATGC CAAGCAACTT CTCCGTAAAT TTGGTGTGCC TGAGGAAGAG 
ATTAAGAAGT TGTCCCGCTG GGAAGTGATC GATGTAGTAC GCACAATGTC AACAGAACAA 
GCCCGCTCTG GAGAGGGACC CATGAGTAAA TTTGCCCGTG GATCAAGGTT TTCTGTGGCT 
GAGCATCAAG AGCGTTACAA AGAGGAATGT CAGCGCATCT TTGACCTGCA GAACAAGGTT 
TTATCATCAA CTGAAATCTT ATCAACTGAC ACAGACAGCA GCTCAGCTGA AGACAGTGAC 
TTTGAGGAAA TGGGAAAAAA CATAGAGAAC ATGTTGCAGA ATAAGAAAAC CAGCTCTCAG 
CTGTCACGTG AACGGGAGGA GCAGGAACGG AAGGAACTAC AGAGGATGAT ACTGGCAGCA 
GGCTCAGCAG CATCAGGAAA CAATCACAGA GATGATGACA CTGCTTCTGT GACAAGCCTT 
AATTCTTCTG CCACTGGCCG TTGTCTCAAG ATTTATCGCA CGTTTCGAGA TGAGGAGGGG 
AAAGAATATG TTCGCTGTGA GACGGTCCGA AAGCCAGCTG TCATTGATGC CTATGTACGC 
ATACGGACCA CAAAAGACGA GGAATTCATT CGAAAATTTG CCCTTTTTGA TGAACAACAT 
CGAGAAGAGA TGCGAAAAGA ACGGCGGAGG ATTCAAGAGC AACTGAGACG ACTTAAGCGA 
AACCAGGAAA AGGAGAAGCT TAAGGGTCCT CCTGAGAAGA AGCCCAAAAA AATGAAGGAG 
CGTCCTGACC TAAAACTGAA ATGTGGGGCT TGTGGTGCCA TTGGGCACAT GAGAACAAAC 
AAATTCTGCC CTCTTTATTA TCAAACAAAT GCTCCCCCTT CCAACCCTGT TGCCATGACA 
GAGGAGCAGG AGGAGGAGTT GGAAAAGACA GTCATTCATA ATGATAATGA GGAACTTATC 
AAGGTTGAAG GGACCAAGAT TGTCCTAGGG AAACAGCTAA TTGAGAGTGC AGATGAGGTT 
CGCAGAAAAT CTCTGGTTCT CAAATTCCCT AAGCAGCAAC TTCCTCCCAA GAAGAAACGG 
CGAGTTGGAA CTACTGTTCA CTGTGACTAT CTGAATAGAC CTCATAAGTC CATTCACCGG 
CGCCGGACAG ACCCCATGGT GACATTGTCA TCCATTTTGG AGTCTATCAT CAATGACATG 
AGAGATCTTC CGAATACATA CCCTTTCCAC ACTCCAGTCA ATGCAAAGGT CGTAAAGGAC 
TACTACAAGA TCATCACCCG GCCAATGGAC TTACAGACAC TCCGTGAAAA TGTGCGTAAA 
CGCCTCTACC CATCTCGGGA AGAGTTCAGG GAGCATTTGG AGCTAATTGT GAAAAATAGT 
GCAACCTACA ATGGGCCAAA GCACTCACTG ACCCAGATCT CTCAATCAAT GCTGGATCTC 
TGTGATGAAA AACTTAAAGA GAAAGAAGAT AAATTGGCTC GTTTAGAGAA AGCAATCAAC 
CCCTTGCTGG ATGATGATGA CCAAGTGGCA TTTTCTTTTA TTCTGGACAA CATTGTCACC 
CAGAAAATGA TGGCAGTTCC AGATTCTTGG CCGTTTCATC ACCCAGTTAA TAAGAAGTTT 
GTTCCAGATT ATTACAAAGT AATTGTCAGT CCAATGGATT TAGAGACCAT ACGTAAAAAT 
ATCTCCAAGC ACAAGTATCA GAGTCGGGAG AGCTTTCTAG ACGATGTCAA CCTTATTCTG 
GCCAACAGTG TTAAATACAA TGGGCCTGAA AGTCAGTATA CTAAGACTGC TCAGGAGATT 
GTGAACGTCT GTTACCAGAC ACTGACTGAA TATGATGAGC ATTTAACTCA ACTTGAGAAG 
GATATTTGTA CAGCTAAAGA AGCAGCTTTG GAGGAAGCAG AATTAGAAAG CTTGGACCCA 
ATGACCCCAG GGCCTTACAC ACCTCAGCCT CCTGATTTGT ATGATACCAA CACATCCCTC 
AGTATGTCTC GAGATGCTTC TGTATTTCAA GATGAGAGCA ATATGTCTGT CCTGGATATT 
CCCACTGCCA CTCCAGAAAA GCAGGTGACA CAGATGCACC AGGGCCGAGG TAGGCTGGGC 
GAGGAAGACT CTGATGTAGA TATTGAAGGG TATGATGATG AGGAGGAGGA TGGGAAACCT 
AAGACACCAG CCCCAGAAGG TGAAGATGCA GATGGTGATC TTGCAGATGA AGAGGAAGGA 
ACTGTGCAAC AGCCTCAAGC CAGTGTCCTG TATGAGGATT TGCTTATGTC TGAAGGAGAA 
GATGATGAGG AAGATGCTGG GAGTGATGAA GAAGGAGATA ATCCTTTCTC TGCTATCCAG 
CTGAGTGAAA GTGGAAGTGA CTCGGATGTA GGATCTGGTG GGATAAGACC CAAACAGCCC 
CGCATGCTTC AAGAAAACAC AAGGATGGGC ATGGAAAATG AAGAAAGCAT GATGTCCTAT 
GAGGGAGACG GTGGGGAGGC TTCTCATGGT TTGGAGGATA GCAATATCAG TTATGGGAGC 
TATGAGGAGC CTGATCCCAA GTCGAACACC CAAGACACAA GCTTCAGCAG CATCGGTGGG 
TATGAGGTAT CAGAGGAGGA AGAAGATGAG GAGGAAGAGC AACGCTCTGG GCCGAGCGTA 
CTAAGCCAGG TCCAGCTGTC AGAGGACGAG GAGGACAGTG AGGATTTCCA CTCCATTGCT 
GGGGACAGTG ACTTGGACTC TGATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005228046.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228103.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X3, mRNA.

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CloneID	OBa13031
Clone ID Related Accession (Same CDS sequence)	XM_005200740.3 , XM_005228046.4 , XM_005228046.3
Accession Version	XM_005228046.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228046.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGAGAAAGTG 
GACTTCAGTA GTTCCTCTGA CTCAGAATCT GAGATGGGAC CTCAGGAAGC AGCACAGACA 
GAATCTGAGG ATGGAAAGCT GACCCTACCA TTGGCTGGGA TTATGCAGCA CGATGCCACC 
AAGCTGTTGC CGAGTGTCAC GGAACTTTTC CCAGAATTTC GTCCTGGGAA GGTGTTACGC 
TTCCTACGTC TTTTTGGACC AGGAAAGAAT GTCCCATCTG TTTGGCGCAG TGCTCGGAGA 
AAGAGGAAGA AGAAACACCG TGAACTGATA CAGGAAGAGC AGATCCAGGA GACGGAGTGC 
TCAGTAGAAT CAGAAGTCAA CCAGAAATCT TTGTGGAACT ATGACTATGC CCCACCACCA 
CCTCCAGAGC AGTGTCTCTC TGATGATGAA ATCACAATGA TGGCACCTGT GGAATCCAAG 
TTTTCCCAGT CAACTGGAGA TACAGATAAA GTGACAGACA CCAAACCAAG AGTGGCTGAG 
TGGCGTTATG GGCCTGCTCG ACTGTGGTAT GATATGCTGG GTGTCCCTGA AGATGGCAGT 
GGGTTTGACT ATGGCTTCAA ACTAAGAAAG ATGGAACAGG AACCTGTGAT GAAATGTAGA 
ATGGCGGATG TAAGCAACAC GGAATACAGG ATACCTGAGG AAAGCAGTGG CACCGATCTT 
CTGGCTGATG AAAACTTCCT GATGGTGACA CAGCTGCATT GGGAAGATGA TATCATCTGG 
GATGGGGAGG ATGTTAAACA CAAAGGGACA AAACCACAGC GTGCTAGCCT GGCAGGCTGG 
CTTCCTTCCA GCATGACTAG GAATGCCATG GCTTACAATG TTCAACAAGG TTTTGCAGCA 
ACGCTGGATG ATGACAAGCC TTGGTACTCC ATTTTTCCCA TTGACAATGA GGAGCTGGTA 
TATGGACGTT GGGAGGACAA TATCATTTGG GATGCTCAGG CCATGCCCCG GTTCTTGGAG 
CCTCCTATTT TGACACTTGA TCCCAATGAT GAGAACCTCA TTTTGGAAAT TCCAGATGAG 
AAGGAAGAGG CTACTTCTAA CTCTCCCTCC AAGGAGAGTA AGAAGGAATC ATCTCTGAAG 
AAGAGTCGAA TTCTGTTAGG GAAAACAGGA GTCATCAAGG AGGAACCACA GCAGAACATG 
TCTCAACCAG AAGTGAAAGA TCCATGGAAT CTCTCCAATG ATGAATATTA CTACCCTAAG 
CAACAGGGTC TTCGAGGCAC CTTTGGAGGA AATATTATTC AGCATTCAAT TCCTGCTGTG 
GAATTACGGC AGCCCTTCTT TCCTACCCAC ATGGGGCCCA TCAAACTCCG GCAGTTCCAT 
CGCCCACCTC TGAAGAAGTA CTCTTTTGGG GCACTATCTC AGCCAGGTCC CCACTCAGTC 
CAGCCCTTGC TAAAGCACAT CAAAAAGAAG GCAAAGATGA GAGAACAAGA GAGGCAAGCT 
TCAGGTGGTG GAGAGATGTT TTTCATGCGC ACACCTCAGG ACCTCACAGG CAAAGATGGA 
GATCTTATTC TTGCAGAATA TAGTGAGGAA AACGGACCAT TAATGATGCA AGTTGGCATG 
GCAACCAAGA TAAAAAACTA CTATAAGCGG AAACCCGGAA AAGATCCTGG AGCCCCAGAT 
TGTAAATATG GAGAAACTGT TTACTGCCAT ACATCTCCTT TTCTAGGCTC ACTCCATCCT 
GGACAATTGC TGCAGGCATT TGAGAACAAC CTTTTTCGTG CTCCAATTTA TCTTCATAAG 
ATGCCAGAAA CTGACTTCCT TATTATTCGC ACAAGACAAG GTTACTATAT TCGGGAATTA 
GTGGATATTT TTGTGGTTGG CCAGCAGTGC CCTTTGTTTG AAGTTCCTGG GCCCAACTCC 
AAAAGGGCCA ATACTCATAT TCGAGACTTT CTCCAGGTTT TTATTTATCG CCTGTTCTGG 
AAGAGTAAAG ATCGCCCACG ACGGATCCGA ATGGAAGATA TAAAAAAAGC CTTTCCTTCT 
CACTCAGAAA GCAGCATTCG GAAGAGGCTA AAGCTCTGCG CTGACTTCAA ACGCACAGGC 
ATGGATTCAA ACTGGTGGGT TCTGAAGTCT GATTTTCGTT TACCAACTGA AGAGGAGATC 
AGGGCTATGG TGTCTCCAGA GCAATGCTGT GCTTATTACA GCATGATAGC TGCAGAGCAG 
CGACTGAAGG ATGCTGGCTA TGGTGAGAAG TCCTTTTTTG CTCCAGAAGA GGAAAATGAG 
GAAGATTTCC AGATGAAGAT TGATGATGAG GTTCGCACTG CTCCTTGGAA CACCACACGG 
GCTTTCATTG CTGCCATGAA GGGCAAGTGT CTCCTGGAGG TGACTGGGGT GGCAGATCCC 
ACAGGGTGTG GTGAAGGATT CTCCTATGTG AAGATTCCAA ACAAACCAAC ACAGCAGAAG 
GATGATAAGG AGCCTCAGCC AGTGAAGAAG ACAGTGACAG GAACAGACGC AGACCTCCGT 
CGCCTTTCAC TGAAAAATGC CAAGCAACTT CTCCGTAAAT TTGGTGTGCC TGAGGAAGAG 
ATTAAGAAGT TGTCCCGCTG GGAAGTGATC GATGTAGTAC GCACAATGTC AACAGAACAA 
GCCCGCTCTG GAGAGGGACC CATGAGTAAA TTTGCCCGTG GATCAAGGTT TTCTGTGGCT 
GAGCATCAAG AGCGTTACAA AGAGGAATGT CAGCGCATCT TTGACCTGCA GAACAAGGTT 
TTATCATCAA CTGAAATCTT ATCAACTGAC ACAGACAGCA GCTCAGCTGA AGACAGTGAC 
TTTGAGGAAA TGGGAAAAAA CATAGAGAAC ATGTTGCAGA ATAAGAAAAC CAGCTCTCAG 
CTGTCACGTG AACGGGAGGA GCAGGAACGG AAGGAACTAC AGAGGATGAT ACTGGCAGCA 
GGCTCAGCAG CATCAGGAAA CAATCACAGA GATGATGACA CTGCTTCTGT GACAAGCCTT 
AATTCTTCTG CCACTGGCCG TTGTCTCAAG ATTTATCGCA CGTTTCGAGA TGAGGAGGGG 
AAAGAATATG TTCGCTGTGA GACGGTCCGA AAGCCAGCTG TCATTGATGC CTATGTACGC 
ATACGGACCA CAAAAGACGA GGAATTCATT CGAAAATTTG CCCTTTTTGA TGAACAACAT 
CGAGAAGAGA TGCGAAAAGA ACGGCGGAGG ATTCAAGAGC AACTGAGACG ACTTAAGCGA 
AACCAGGAAA AGGAGAAGCT TAAGGGTCCT CCTGAGAAGA AGCCCAAAAA AATGAAGGAG 
CGTCCTGACC TAAAACTGAA ATGTGGGGCT TGTGGTGCCA TTGGGCACAT GAGAACAAAC 
AAATTCTGCC CTCTTTATTA TCAAACAAAT GCTCCCCCTT CCAACCCTGT TGCCATGACA 
GAGGAGCAGG AGGAGGAGTT GGAAAAGACA GTCATTCATA ATGATAATGA GGAACTTATC 
AAGGTTGAAG GGACCAAGAT TGTCCTAGGG AAACAGCTAA TTGAGAGTGC AGATGAGGTT 
CGCAGAAAAT CTCTGGTTCT CAAATTCCCT AAGCAGCAAC TTCCTCCCAA GAAGAAACGG 
CGAGTTGGAA CTACTGTTCA CTGTGACTAT CTGAATAGAC CTCATAAGTC CATTCACCGG 
CGCCGGACAG ACCCCATGGT GACATTGTCA TCCATTTTGG AGTCTATCAT CAATGACATG 
AGAGATCTTC CGAATACATA CCCTTTCCAC ACTCCAGTCA ATGCAAAGGT CGTAAAGGAC 
TACTACAAGA TCATCACCCG GCCAATGGAC TTACAGACAC TCCGTGAAAA TGTGCGTAAA 
CGCCTCTACC CATCTCGGGA AGAGTTCAGG GAGCATTTGG AGCTAATTGT GAAAAATAGT 
GCAACCTACA ATGGGCCAAA GCACTCACTG ACCCAGATCT CTCAATCAAT GCTGGATCTC 
TGTGATGAAA AACTTAAAGA GAAAGAAGAT AAATTGGCTC GTTTAGAGAA AGCAATCAAC 
CCCTTGCTGG ATGATGATGA CCAAGTGGCA TTTTCTTTTA TTCTGGACAA CATTGTCACC 
CAGAAAATGA TGGCAGTTCC AGATTCTTGG CCGTTTCATC ACCCAGTTAA TAAGAAGTTT 
GTTCCAGATT ATTACAAAGT AATTGTCAGT CCAATGGATT TAGAGACCAT ACGTAAAAAT 
ATCTCCAAGC ACAAGTATCA GAGTCGGGAG AGCTTTCTAG ACGATGTCAA CCTTATTCTG 
GCCAACAGTG TTAAATACAA TGGGCCTGAA AGTCAGTATA CTAAGACTGC TCAGGAGATT 
GTGAACGTCT GTTACCAGAC ACTGACTGAA TATGATGAGC ATTTAACTCA ACTTGAGAAG 
GATATTTGTA CAGCTAAAGA AGCAGCTTTG GAGGAAGCAG AATTAGAAAG CTTGGACCCA 
ATGACCCCAG GGCCTTACAC ACCTCAGCCT CCTGATTTGT ATGATACCAA CACATCCCTC 
AGTATGTCTC GAGATGCTTC TGTATTTCAA GATGAGAGCA ATATGTCTGT CCTGGATATT 
CCCACTGCCA CTCCAGAAAA GCAGGTGACA CAGATGCACC AGGGCCGAGG TAGGCTGGGC 
GAGGAAGACT CTGATGTAGA TATTGAAGGG TATGATGATG AGGAGGAGGA TGGGAAACCT 
AAGACACCAG CCCCAGAAGG TGAAGATGCA GATGGTGATC TTGCAGATGA AGAGGAAGGA 
ACTGTGCAAC AGCCTCAAGC CAGTGTCCTG TATGAGGATT TGCTTATGTC TGAAGGAGAA 
GATGATGAGG AAGATGCTGG GAGTGATGAA GAAGGAGATA ATCCTTTCTC TGCTATCCAG 
CTGAGTGAAA GTGGAAGTGA CTCGGATGTA GGATCTGGTG GGATAAGACC CAAACAGCCC 
CGCATGCTTC AAGAAAACAC AAGGATGGGC ATGGAAAATG AAGAAAGCAT GATGTCCTAT 
GAGGGAGACG GTGGGGAGGC TTCTCATGGT TTGGAGGATA GCAATATCAG TTATGGGAGC 
TATGAGGAGC CTGATCCCAA GTCGAACACC CAAGACACAA GCTTCAGCAG CATCGGTGGG 
TATGAGGTAT CAGAGGAGGA AGAAGATGAG GAGGAAGAGC AACGCTCTGG GCCGAGCGTA 
CTAAGCCAGG TCCAGCTGTC AGAGGACGAG GAGGACAGTG AGGATTTCCA CTCCATTGCT 
GGGGACAGTG ACTTGGACTC TGATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228103.1
CDS	99..5768
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQMHQGRGRLGEEDSDVDIEGYDDEEEDGKPKTPAPEGEDADGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVQLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_005228046.4
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228046.4

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGAGAAAGTG 
GACTTCAGTA GTTCCTCTGA CTCAGAATCT GAGATGGGAC CTCAGGAAGC AGCACAGACA 
GAATCTGAGG ATGGAAAGCT GACCCTACCA TTGGCTGGGA TTATGCAGCA CGATGCCACC 
AAGCTGTTGC CGAGTGTCAC GGAACTTTTC CCAGAATTTC GTCCTGGGAA GGTGTTACGC 
TTCCTACGTC TTTTTGGACC AGGAAAGAAT GTCCCATCTG TTTGGCGCAG TGCTCGGAGA 
AAGAGGAAGA AGAAACACCG TGAACTGATA CAGGAAGAGC AGATCCAGGA GACGGAGTGC 
TCAGTAGAAT CAGAAGTCAA CCAGAAATCT TTGTGGAACT ATGACTATGC CCCACCACCA 
CCTCCAGAGC AGTGTCTCTC TGATGATGAA ATCACAATGA TGGCACCTGT GGAATCCAAG 
TTTTCCCAGT CAACTGGAGA TACAGATAAA GTGACAGACA CCAAACCAAG AGTGGCTGAG 
TGGCGTTATG GGCCTGCTCG ACTGTGGTAT GATATGCTGG GTGTCCCTGA AGATGGCAGT 
GGGTTTGACT ATGGCTTCAA ACTAAGAAAG ATGGAACAGG AACCTGTGAT GAAATGTAGA 
ATGGCGGATG TAAGCAACAC GGAATACAGG ATACCTGAGG AAAGCAGTGG CACCGATCTT 
CTGGCTGATG AAAACTTCCT GATGGTGACA CAGCTGCATT GGGAAGATGA TATCATCTGG 
GATGGGGAGG ATGTTAAACA CAAAGGGACA AAACCACAGC GTGCTAGCCT GGCAGGCTGG 
CTTCCTTCCA GCATGACTAG GAATGCCATG GCTTACAATG TTCAACAAGG TTTTGCAGCA 
ACGCTGGATG ATGACAAGCC TTGGTACTCC ATTTTTCCCA TTGACAATGA GGAGCTGGTA 
TATGGACGTT GGGAGGACAA TATCATTTGG GATGCTCAGG CCATGCCCCG GTTCTTGGAG 
CCTCCTATTT TGACACTTGA TCCCAATGAT GAGAACCTCA TTTTGGAAAT TCCAGATGAG 
AAGGAAGAGG CTACTTCTAA CTCTCCCTCC AAGGAGAGTA AGAAGGAATC ATCTCTGAAG 
AAGAGTCGAA TTCTGTTAGG GAAAACAGGA GTCATCAAGG AGGAACCACA GCAGAACATG 
TCTCAACCAG AAGTGAAAGA TCCATGGAAT CTCTCCAATG ATGAATATTA CTACCCTAAG 
CAACAGGGTC TTCGAGGCAC CTTTGGAGGA AATATTATTC AGCATTCAAT TCCTGCTGTG 
GAATTACGGC AGCCCTTCTT TCCTACCCAC ATGGGGCCCA TCAAACTCCG GCAGTTCCAT 
CGCCCACCTC TGAAGAAGTA CTCTTTTGGG GCACTATCTC AGCCAGGTCC CCACTCAGTC 
CAGCCCTTGC TAAAGCACAT CAAAAAGAAG GCAAAGATGA GAGAACAAGA GAGGCAAGCT 
TCAGGTGGTG GAGAGATGTT TTTCATGCGC ACACCTCAGG ACCTCACAGG CAAAGATGGA 
GATCTTATTC TTGCAGAATA TAGTGAGGAA AACGGACCAT TAATGATGCA AGTTGGCATG 
GCAACCAAGA TAAAAAACTA CTATAAGCGG AAACCCGGAA AAGATCCTGG AGCCCCAGAT 
TGTAAATATG GAGAAACTGT TTACTGCCAT ACATCTCCTT TTCTAGGCTC ACTCCATCCT 
GGACAATTGC TGCAGGCATT TGAGAACAAC CTTTTTCGTG CTCCAATTTA TCTTCATAAG 
ATGCCAGAAA CTGACTTCCT TATTATTCGC ACAAGACAAG GTTACTATAT TCGGGAATTA 
GTGGATATTT TTGTGGTTGG CCAGCAGTGC CCTTTGTTTG AAGTTCCTGG GCCCAACTCC 
AAAAGGGCCA ATACTCATAT TCGAGACTTT CTCCAGGTTT TTATTTATCG CCTGTTCTGG 
AAGAGTAAAG ATCGCCCACG ACGGATCCGA ATGGAAGATA TAAAAAAAGC CTTTCCTTCT 
CACTCAGAAA GCAGCATTCG GAAGAGGCTA AAGCTCTGCG CTGACTTCAA ACGCACAGGC 
ATGGATTCAA ACTGGTGGGT TCTGAAGTCT GATTTTCGTT TACCAACTGA AGAGGAGATC 
AGGGCTATGG TGTCTCCAGA GCAATGCTGT GCTTATTACA GCATGATAGC TGCAGAGCAG 
CGACTGAAGG ATGCTGGCTA TGGTGAGAAG TCCTTTTTTG CTCCAGAAGA GGAAAATGAG 
GAAGATTTCC AGATGAAGAT TGATGATGAG GTTCGCACTG CTCCTTGGAA CACCACACGG 
GCTTTCATTG CTGCCATGAA GGGCAAGTGT CTCCTGGAGG TGACTGGGGT GGCAGATCCC 
ACAGGGTGTG GTGAAGGATT CTCCTATGTG AAGATTCCAA ACAAACCAAC ACAGCAGAAG 
GATGATAAGG AGCCTCAGCC AGTGAAGAAG ACAGTGACAG GAACAGACGC AGACCTCCGT 
CGCCTTTCAC TGAAAAATGC CAAGCAACTT CTCCGTAAAT TTGGTGTGCC TGAGGAAGAG 
ATTAAGAAGT TGTCCCGCTG GGAAGTGATC GATGTAGTAC GCACAATGTC AACAGAACAA 
GCCCGCTCTG GAGAGGGACC CATGAGTAAA TTTGCCCGTG GATCAAGGTT TTCTGTGGCT 
GAGCATCAAG AGCGTTACAA AGAGGAATGT CAGCGCATCT TTGACCTGCA GAACAAGGTT 
TTATCATCAA CTGAAATCTT ATCAACTGAC ACAGACAGCA GCTCAGCTGA AGACAGTGAC 
TTTGAGGAAA TGGGAAAAAA CATAGAGAAC ATGTTGCAGA ATAAGAAAAC CAGCTCTCAG 
CTGTCACGTG AACGGGAGGA GCAGGAACGG AAGGAACTAC AGAGGATGAT ACTGGCAGCA 
GGCTCAGCAG CATCAGGAAA CAATCACAGA GATGATGACA CTGCTTCTGT GACAAGCCTT 
AATTCTTCTG CCACTGGCCG TTGTCTCAAG ATTTATCGCA CGTTTCGAGA TGAGGAGGGG 
AAAGAATATG TTCGCTGTGA GACGGTCCGA AAGCCAGCTG TCATTGATGC CTATGTACGC 
ATACGGACCA CAAAAGACGA GGAATTCATT CGAAAATTTG CCCTTTTTGA TGAACAACAT 
CGAGAAGAGA TGCGAAAAGA ACGGCGGAGG ATTCAAGAGC AACTGAGACG ACTTAAGCGA 
AACCAGGAAA AGGAGAAGCT TAAGGGTCCT CCTGAGAAGA AGCCCAAAAA AATGAAGGAG 
CGTCCTGACC TAAAACTGAA ATGTGGGGCT TGTGGTGCCA TTGGGCACAT GAGAACAAAC 
AAATTCTGCC CTCTTTATTA TCAAACAAAT GCTCCCCCTT CCAACCCTGT TGCCATGACA 
GAGGAGCAGG AGGAGGAGTT GGAAAAGACA GTCATTCATA ATGATAATGA GGAACTTATC 
AAGGTTGAAG GGACCAAGAT TGTCCTAGGG AAACAGCTAA TTGAGAGTGC AGATGAGGTT 
CGCAGAAAAT CTCTGGTTCT CAAATTCCCT AAGCAGCAAC TTCCTCCCAA GAAGAAACGG 
CGAGTTGGAA CTACTGTTCA CTGTGACTAT CTGAATAGAC CTCATAAGTC CATTCACCGG 
CGCCGGACAG ACCCCATGGT GACATTGTCA TCCATTTTGG AGTCTATCAT CAATGACATG 
AGAGATCTTC CGAATACATA CCCTTTCCAC ACTCCAGTCA ATGCAAAGGT CGTAAAGGAC 
TACTACAAGA TCATCACCCG GCCAATGGAC TTACAGACAC TCCGTGAAAA TGTGCGTAAA 
CGCCTCTACC CATCTCGGGA AGAGTTCAGG GAGCATTTGG AGCTAATTGT GAAAAATAGT 
GCAACCTACA ATGGGCCAAA GCACTCACTG ACCCAGATCT CTCAATCAAT GCTGGATCTC 
TGTGATGAAA AACTTAAAGA GAAAGAAGAT AAATTGGCTC GTTTAGAGAA AGCAATCAAC 
CCCTTGCTGG ATGATGATGA CCAAGTGGCA TTTTCTTTTA TTCTGGACAA CATTGTCACC 
CAGAAAATGA TGGCAGTTCC AGATTCTTGG CCGTTTCATC ACCCAGTTAA TAAGAAGTTT 
GTTCCAGATT ATTACAAAGT AATTGTCAGT CCAATGGATT TAGAGACCAT ACGTAAAAAT 
ATCTCCAAGC ACAAGTATCA GAGTCGGGAG AGCTTTCTAG ACGATGTCAA CCTTATTCTG 
GCCAACAGTG TTAAATACAA TGGGCCTGAA AGTCAGTATA CTAAGACTGC TCAGGAGATT 
GTGAACGTCT GTTACCAGAC ACTGACTGAA TATGATGAGC ATTTAACTCA ACTTGAGAAG 
GATATTTGTA CAGCTAAAGA AGCAGCTTTG GAGGAAGCAG AATTAGAAAG CTTGGACCCA 
ATGACCCCAG GGCCTTACAC ACCTCAGCCT CCTGATTTGT ATGATACCAA CACATCCCTC 
AGTATGTCTC GAGATGCTTC TGTATTTCAA GATGAGAGCA ATATGTCTGT CCTGGATATT 
CCCACTGCCA CTCCAGAAAA GCAGGTGACA CAGATGCACC AGGGCCGAGG TAGGCTGGGC 
GAGGAAGACT CTGATGTAGA TATTGAAGGG TATGATGATG AGGAGGAGGA TGGGAAACCT 
AAGACACCAG CCCCAGAAGG TGAAGATGCA GATGGTGATC TTGCAGATGA AGAGGAAGGA 
ACTGTGCAAC AGCCTCAAGC CAGTGTCCTG TATGAGGATT TGCTTATGTC TGAAGGAGAA 
GATGATGAGG AAGATGCTGG GAGTGATGAA GAAGGAGATA ATCCTTTCTC TGCTATCCAG 
CTGAGTGAAA GTGGAAGTGA CTCGGATGTA GGATCTGGTG GGATAAGACC CAAACAGCCC 
CGCATGCTTC AAGAAAACAC AAGGATGGGC ATGGAAAATG AAGAAAGCAT GATGTCCTAT 
GAGGGAGACG GTGGGGAGGC TTCTCATGGT TTGGAGGATA GCAATATCAG TTATGGGAGC 
TATGAGGAGC CTGATCCCAA GTCGAACACC CAAGACACAA GCTTCAGCAG CATCGGTGGG 
TATGAGGTAT CAGAGGAGGA AGAAGATGAG GAGGAAGAGC AACGCTCTGG GCCGAGCGTA 
CTAAGCCAGG TCCAGCTGTC AGAGGACGAG GAGGACAGTG AGGATTTCCA CTCCATTGCT 
GGGGACAGTG ACTTGGACTC TGATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005228046.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228103.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X2, mRNA.

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CloneID	OBa13031
Clone ID Related Accession (Same CDS sequence)	XM_005200740.3 , XM_005228046.4 , XM_005228046.3
Accession Version	XM_005228046.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104728.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228046.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGAGAAAGTG 
GACTTCAGTA GTTCCTCTGA CTCAGAATCT GAGATGGGAC CTCAGGAAGC AGCACAGACA 
GAATCTGAGG ATGGAAAGCT GACCCTACCA TTGGCTGGGA TTATGCAGCA CGATGCCACC 
AAGCTGTTGC CGAGTGTCAC GGAACTTTTC CCAGAATTTC GTCCTGGGAA GGTGTTACGC 
TTCCTACGTC TTTTTGGACC AGGAAAGAAT GTCCCATCTG TTTGGCGCAG TGCTCGGAGA 
AAGAGGAAGA AGAAACACCG TGAACTGATA CAGGAAGAGC AGATCCAGGA GACGGAGTGC 
TCAGTAGAAT CAGAAGTCAA CCAGAAATCT TTGTGGAACT ATGACTATGC CCCACCACCA 
CCTCCAGAGC AGTGTCTCTC TGATGATGAA ATCACAATGA TGGCACCTGT GGAATCCAAG 
TTTTCCCAGT CAACTGGAGA TACAGATAAA GTGACAGACA CCAAACCAAG AGTGGCTGAG 
TGGCGTTATG GGCCTGCTCG ACTGTGGTAT GATATGCTGG GTGTCCCTGA AGATGGCAGT 
GGGTTTGACT ATGGCTTCAA ACTAAGAAAG ATGGAACAGG AACCTGTGAT GAAATGTAGA 
ATGGCGGATG TAAGCAACAC GGAATACAGG ATACCTGAGG AAAGCAGTGG CACCGATCTT 
CTGGCTGATG AAAACTTCCT GATGGTGACA CAGCTGCATT GGGAAGATGA TATCATCTGG 
GATGGGGAGG ATGTTAAACA CAAAGGGACA AAACCACAGC GTGCTAGCCT GGCAGGCTGG 
CTTCCTTCCA GCATGACTAG GAATGCCATG GCTTACAATG TTCAACAAGG TTTTGCAGCA 
ACGCTGGATG ATGACAAGCC TTGGTACTCC ATTTTTCCCA TTGACAATGA GGAGCTGGTA 
TATGGACGTT GGGAGGACAA TATCATTTGG GATGCTCAGG CCATGCCCCG GTTCTTGGAG 
CCTCCTATTT TGACACTTGA TCCCAATGAT GAGAACCTCA TTTTGGAAAT TCCAGATGAG 
AAGGAAGAGG CTACTTCTAA CTCTCCCTCC AAGGAGAGTA AGAAGGAATC ATCTCTGAAG 
AAGAGTCGAA TTCTGTTAGG GAAAACAGGA GTCATCAAGG AGGAACCACA GCAGAACATG 
TCTCAACCAG AAGTGAAAGA TCCATGGAAT CTCTCCAATG ATGAATATTA CTACCCTAAG 
CAACAGGGTC TTCGAGGCAC CTTTGGAGGA AATATTATTC AGCATTCAAT TCCTGCTGTG 
GAATTACGGC AGCCCTTCTT TCCTACCCAC ATGGGGCCCA TCAAACTCCG GCAGTTCCAT 
CGCCCACCTC TGAAGAAGTA CTCTTTTGGG GCACTATCTC AGCCAGGTCC CCACTCAGTC 
CAGCCCTTGC TAAAGCACAT CAAAAAGAAG GCAAAGATGA GAGAACAAGA GAGGCAAGCT 
TCAGGTGGTG GAGAGATGTT TTTCATGCGC ACACCTCAGG ACCTCACAGG CAAAGATGGA 
GATCTTATTC TTGCAGAATA TAGTGAGGAA AACGGACCAT TAATGATGCA AGTTGGCATG 
GCAACCAAGA TAAAAAACTA CTATAAGCGG AAACCCGGAA AAGATCCTGG AGCCCCAGAT 
TGTAAATATG GAGAAACTGT TTACTGCCAT ACATCTCCTT TTCTAGGCTC ACTCCATCCT 
GGACAATTGC TGCAGGCATT TGAGAACAAC CTTTTTCGTG CTCCAATTTA TCTTCATAAG 
ATGCCAGAAA CTGACTTCCT TATTATTCGC ACAAGACAAG GTTACTATAT TCGGGAATTA 
GTGGATATTT TTGTGGTTGG CCAGCAGTGC CCTTTGTTTG AAGTTCCTGG GCCCAACTCC 
AAAAGGGCCA ATACTCATAT TCGAGACTTT CTCCAGGTTT TTATTTATCG CCTGTTCTGG 
AAGAGTAAAG ATCGCCCACG ACGGATCCGA ATGGAAGATA TAAAAAAAGC CTTTCCTTCT 
CACTCAGAAA GCAGCATTCG GAAGAGGCTA AAGCTCTGCG CTGACTTCAA ACGCACAGGC 
ATGGATTCAA ACTGGTGGGT TCTGAAGTCT GATTTTCGTT TACCAACTGA AGAGGAGATC 
AGGGCTATGG TGTCTCCAGA GCAATGCTGT GCTTATTACA GCATGATAGC TGCAGAGCAG 
CGACTGAAGG ATGCTGGCTA TGGTGAGAAG TCCTTTTTTG CTCCAGAAGA GGAAAATGAG 
GAAGATTTCC AGATGAAGAT TGATGATGAG GTTCGCACTG CTCCTTGGAA CACCACACGG 
GCTTTCATTG CTGCCATGAA GGGCAAGTGT CTCCTGGAGG TGACTGGGGT GGCAGATCCC 
ACAGGGTGTG GTGAAGGATT CTCCTATGTG AAGATTCCAA ACAAACCAAC ACAGCAGAAG 
GATGATAAGG AGCCTCAGCC AGTGAAGAAG ACAGTGACAG GAACAGACGC AGACCTCCGT 
CGCCTTTCAC TGAAAAATGC CAAGCAACTT CTCCGTAAAT TTGGTGTGCC TGAGGAAGAG 
ATTAAGAAGT TGTCCCGCTG GGAAGTGATC GATGTAGTAC GCACAATGTC AACAGAACAA 
GCCCGCTCTG GAGAGGGACC CATGAGTAAA TTTGCCCGTG GATCAAGGTT TTCTGTGGCT 
GAGCATCAAG AGCGTTACAA AGAGGAATGT CAGCGCATCT TTGACCTGCA GAACAAGGTT 
TTATCATCAA CTGAAATCTT ATCAACTGAC ACAGACAGCA GCTCAGCTGA AGACAGTGAC 
TTTGAGGAAA TGGGAAAAAA CATAGAGAAC ATGTTGCAGA ATAAGAAAAC CAGCTCTCAG 
CTGTCACGTG AACGGGAGGA GCAGGAACGG AAGGAACTAC AGAGGATGAT ACTGGCAGCA 
GGCTCAGCAG CATCAGGAAA CAATCACAGA GATGATGACA CTGCTTCTGT GACAAGCCTT 
AATTCTTCTG CCACTGGCCG TTGTCTCAAG ATTTATCGCA CGTTTCGAGA TGAGGAGGGG 
AAAGAATATG TTCGCTGTGA GACGGTCCGA AAGCCAGCTG TCATTGATGC CTATGTACGC 
ATACGGACCA CAAAAGACGA GGAATTCATT CGAAAATTTG CCCTTTTTGA TGAACAACAT 
CGAGAAGAGA TGCGAAAAGA ACGGCGGAGG ATTCAAGAGC AACTGAGACG ACTTAAGCGA 
AACCAGGAAA AGGAGAAGCT TAAGGGTCCT CCTGAGAAGA AGCCCAAAAA AATGAAGGAG 
CGTCCTGACC TAAAACTGAA ATGTGGGGCT TGTGGTGCCA TTGGGCACAT GAGAACAAAC 
AAATTCTGCC CTCTTTATTA TCAAACAAAT GCTCCCCCTT CCAACCCTGT TGCCATGACA 
GAGGAGCAGG AGGAGGAGTT GGAAAAGACA GTCATTCATA ATGATAATGA GGAACTTATC 
AAGGTTGAAG GGACCAAGAT TGTCCTAGGG AAACAGCTAA TTGAGAGTGC AGATGAGGTT 
CGCAGAAAAT CTCTGGTTCT CAAATTCCCT AAGCAGCAAC TTCCTCCCAA GAAGAAACGG 
CGAGTTGGAA CTACTGTTCA CTGTGACTAT CTGAATAGAC CTCATAAGTC CATTCACCGG 
CGCCGGACAG ACCCCATGGT GACATTGTCA TCCATTTTGG AGTCTATCAT CAATGACATG 
AGAGATCTTC CGAATACATA CCCTTTCCAC ACTCCAGTCA ATGCAAAGGT CGTAAAGGAC 
TACTACAAGA TCATCACCCG GCCAATGGAC TTACAGACAC TCCGTGAAAA TGTGCGTAAA 
CGCCTCTACC CATCTCGGGA AGAGTTCAGG GAGCATTTGG AGCTAATTGT GAAAAATAGT 
GCAACCTACA ATGGGCCAAA GCACTCACTG ACCCAGATCT CTCAATCAAT GCTGGATCTC 
TGTGATGAAA AACTTAAAGA GAAAGAAGAT AAATTGGCTC GTTTAGAGAA AGCAATCAAC 
CCCTTGCTGG ATGATGATGA CCAAGTGGCA TTTTCTTTTA TTCTGGACAA CATTGTCACC 
CAGAAAATGA TGGCAGTTCC AGATTCTTGG CCGTTTCATC ACCCAGTTAA TAAGAAGTTT 
GTTCCAGATT ATTACAAAGT AATTGTCAGT CCAATGGATT TAGAGACCAT ACGTAAAAAT 
ATCTCCAAGC ACAAGTATCA GAGTCGGGAG AGCTTTCTAG ACGATGTCAA CCTTATTCTG 
GCCAACAGTG TTAAATACAA TGGGCCTGAA AGTCAGTATA CTAAGACTGC TCAGGAGATT 
GTGAACGTCT GTTACCAGAC ACTGACTGAA TATGATGAGC ATTTAACTCA ACTTGAGAAG 
GATATTTGTA CAGCTAAAGA AGCAGCTTTG GAGGAAGCAG AATTAGAAAG CTTGGACCCA 
ATGACCCCAG GGCCTTACAC ACCTCAGCCT CCTGATTTGT ATGATACCAA CACATCCCTC 
AGTATGTCTC GAGATGCTTC TGTATTTCAA GATGAGAGCA ATATGTCTGT CCTGGATATT 
CCCACTGCCA CTCCAGAAAA GCAGGTGACA CAGATGCACC AGGGCCGAGG TAGGCTGGGC 
GAGGAAGACT CTGATGTAGA TATTGAAGGG TATGATGATG AGGAGGAGGA TGGGAAACCT 
AAGACACCAG CCCCAGAAGG TGAAGATGCA GATGGTGATC TTGCAGATGA AGAGGAAGGA 
ACTGTGCAAC AGCCTCAAGC CAGTGTCCTG TATGAGGATT TGCTTATGTC TGAAGGAGAA 
GATGATGAGG AAGATGCTGG GAGTGATGAA GAAGGAGATA ATCCTTTCTC TGCTATCCAG 
CTGAGTGAAA GTGGAAGTGA CTCGGATGTA GGATCTGGTG GGATAAGACC CAAACAGCCC 
CGCATGCTTC AAGAAAACAC AAGGATGGGC ATGGAAAATG AAGAAAGCAT GATGTCCTAT 
GAGGGAGACG GTGGGGAGGC TTCTCATGGT TTGGAGGATA GCAATATCAG TTATGGGAGC 
TATGAGGAGC CTGATCCCAA GTCGAACACC CAAGACACAA GCTTCAGCAG CATCGGTGGG 
TATGAGGTAT CAGAGGAGGA AGAAGATGAG GAGGAAGAGC AACGCTCTGG GCCGAGCGTA 
CTAAGCCAGG TCCAGCTGTC AGAGGACGAG GAGGACAGTG AGGATTTCCA CTCCATTGCT 
GGGGACAGTG ACTTGGACTC TGATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228103.1
CDS	106..5775
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQMHQGRGRLGEEDSDVDIEGYDDEEEDGKPKTPAPEGEDADGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVQLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_005228046.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228046.3

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGAGAAAGTG 
GACTTCAGTA GTTCCTCTGA CTCAGAATCT GAGATGGGAC CTCAGGAAGC AGCACAGACA 
GAATCTGAGG ATGGAAAGCT GACCCTACCA TTGGCTGGGA TTATGCAGCA CGATGCCACC 
AAGCTGTTGC CGAGTGTCAC GGAACTTTTC CCAGAATTTC GTCCTGGGAA GGTGTTACGC 
TTCCTACGTC TTTTTGGACC AGGAAAGAAT GTCCCATCTG TTTGGCGCAG TGCTCGGAGA 
AAGAGGAAGA AGAAACACCG TGAACTGATA CAGGAAGAGC AGATCCAGGA GACGGAGTGC 
TCAGTAGAAT CAGAAGTCAA CCAGAAATCT TTGTGGAACT ATGACTATGC CCCACCACCA 
CCTCCAGAGC AGTGTCTCTC TGATGATGAA ATCACAATGA TGGCACCTGT GGAATCCAAG 
TTTTCCCAGT CAACTGGAGA TACAGATAAA GTGACAGACA CCAAACCAAG AGTGGCTGAG 
TGGCGTTATG GGCCTGCTCG ACTGTGGTAT GATATGCTGG GTGTCCCTGA AGATGGCAGT 
GGGTTTGACT ATGGCTTCAA ACTAAGAAAG ATGGAACAGG AACCTGTGAT GAAATGTAGA 
ATGGCGGATG TAAGCAACAC GGAATACAGG ATACCTGAGG AAAGCAGTGG CACCGATCTT 
CTGGCTGATG AAAACTTCCT GATGGTGACA CAGCTGCATT GGGAAGATGA TATCATCTGG 
GATGGGGAGG ATGTTAAACA CAAAGGGACA AAACCACAGC GTGCTAGCCT GGCAGGCTGG 
CTTCCTTCCA GCATGACTAG GAATGCCATG GCTTACAATG TTCAACAAGG TTTTGCAGCA 
ACGCTGGATG ATGACAAGCC TTGGTACTCC ATTTTTCCCA TTGACAATGA GGAGCTGGTA 
TATGGACGTT GGGAGGACAA TATCATTTGG GATGCTCAGG CCATGCCCCG GTTCTTGGAG 
CCTCCTATTT TGACACTTGA TCCCAATGAT GAGAACCTCA TTTTGGAAAT TCCAGATGAG 
AAGGAAGAGG CTACTTCTAA CTCTCCCTCC AAGGAGAGTA AGAAGGAATC ATCTCTGAAG 
AAGAGTCGAA TTCTGTTAGG GAAAACAGGA GTCATCAAGG AGGAACCACA GCAGAACATG 
TCTCAACCAG AAGTGAAAGA TCCATGGAAT CTCTCCAATG ATGAATATTA CTACCCTAAG 
CAACAGGGTC TTCGAGGCAC CTTTGGAGGA AATATTATTC AGCATTCAAT TCCTGCTGTG 
GAATTACGGC AGCCCTTCTT TCCTACCCAC ATGGGGCCCA TCAAACTCCG GCAGTTCCAT 
CGCCCACCTC TGAAGAAGTA CTCTTTTGGG GCACTATCTC AGCCAGGTCC CCACTCAGTC 
CAGCCCTTGC TAAAGCACAT CAAAAAGAAG GCAAAGATGA GAGAACAAGA GAGGCAAGCT 
TCAGGTGGTG GAGAGATGTT TTTCATGCGC ACACCTCAGG ACCTCACAGG CAAAGATGGA 
GATCTTATTC TTGCAGAATA TAGTGAGGAA AACGGACCAT TAATGATGCA AGTTGGCATG 
GCAACCAAGA TAAAAAACTA CTATAAGCGG AAACCCGGAA AAGATCCTGG AGCCCCAGAT 
TGTAAATATG GAGAAACTGT TTACTGCCAT ACATCTCCTT TTCTAGGCTC ACTCCATCCT 
GGACAATTGC TGCAGGCATT TGAGAACAAC CTTTTTCGTG CTCCAATTTA TCTTCATAAG 
ATGCCAGAAA CTGACTTCCT TATTATTCGC ACAAGACAAG GTTACTATAT TCGGGAATTA 
GTGGATATTT TTGTGGTTGG CCAGCAGTGC CCTTTGTTTG AAGTTCCTGG GCCCAACTCC 
AAAAGGGCCA ATACTCATAT TCGAGACTTT CTCCAGGTTT TTATTTATCG CCTGTTCTGG 
AAGAGTAAAG ATCGCCCACG ACGGATCCGA ATGGAAGATA TAAAAAAAGC CTTTCCTTCT 
CACTCAGAAA GCAGCATTCG GAAGAGGCTA AAGCTCTGCG CTGACTTCAA ACGCACAGGC 
ATGGATTCAA ACTGGTGGGT TCTGAAGTCT GATTTTCGTT TACCAACTGA AGAGGAGATC 
AGGGCTATGG TGTCTCCAGA GCAATGCTGT GCTTATTACA GCATGATAGC TGCAGAGCAG 
CGACTGAAGG ATGCTGGCTA TGGTGAGAAG TCCTTTTTTG CTCCAGAAGA GGAAAATGAG 
GAAGATTTCC AGATGAAGAT TGATGATGAG GTTCGCACTG CTCCTTGGAA CACCACACGG 
GCTTTCATTG CTGCCATGAA GGGCAAGTGT CTCCTGGAGG TGACTGGGGT GGCAGATCCC 
ACAGGGTGTG GTGAAGGATT CTCCTATGTG AAGATTCCAA ACAAACCAAC ACAGCAGAAG 
GATGATAAGG AGCCTCAGCC AGTGAAGAAG ACAGTGACAG GAACAGACGC AGACCTCCGT 
CGCCTTTCAC TGAAAAATGC CAAGCAACTT CTCCGTAAAT TTGGTGTGCC TGAGGAAGAG 
ATTAAGAAGT TGTCCCGCTG GGAAGTGATC GATGTAGTAC GCACAATGTC AACAGAACAA 
GCCCGCTCTG GAGAGGGACC CATGAGTAAA TTTGCCCGTG GATCAAGGTT TTCTGTGGCT 
GAGCATCAAG AGCGTTACAA AGAGGAATGT CAGCGCATCT TTGACCTGCA GAACAAGGTT 
TTATCATCAA CTGAAATCTT ATCAACTGAC ACAGACAGCA GCTCAGCTGA AGACAGTGAC 
TTTGAGGAAA TGGGAAAAAA CATAGAGAAC ATGTTGCAGA ATAAGAAAAC CAGCTCTCAG 
CTGTCACGTG AACGGGAGGA GCAGGAACGG AAGGAACTAC AGAGGATGAT ACTGGCAGCA 
GGCTCAGCAG CATCAGGAAA CAATCACAGA GATGATGACA CTGCTTCTGT GACAAGCCTT 
AATTCTTCTG CCACTGGCCG TTGTCTCAAG ATTTATCGCA CGTTTCGAGA TGAGGAGGGG 
AAAGAATATG TTCGCTGTGA GACGGTCCGA AAGCCAGCTG TCATTGATGC CTATGTACGC 
ATACGGACCA CAAAAGACGA GGAATTCATT CGAAAATTTG CCCTTTTTGA TGAACAACAT 
CGAGAAGAGA TGCGAAAAGA ACGGCGGAGG ATTCAAGAGC AACTGAGACG ACTTAAGCGA 
AACCAGGAAA AGGAGAAGCT TAAGGGTCCT CCTGAGAAGA AGCCCAAAAA AATGAAGGAG 
CGTCCTGACC TAAAACTGAA ATGTGGGGCT TGTGGTGCCA TTGGGCACAT GAGAACAAAC 
AAATTCTGCC CTCTTTATTA TCAAACAAAT GCTCCCCCTT CCAACCCTGT TGCCATGACA 
GAGGAGCAGG AGGAGGAGTT GGAAAAGACA GTCATTCATA ATGATAATGA GGAACTTATC 
AAGGTTGAAG GGACCAAGAT TGTCCTAGGG AAACAGCTAA TTGAGAGTGC AGATGAGGTT 
CGCAGAAAAT CTCTGGTTCT CAAATTCCCT AAGCAGCAAC TTCCTCCCAA GAAGAAACGG 
CGAGTTGGAA CTACTGTTCA CTGTGACTAT CTGAATAGAC CTCATAAGTC CATTCACCGG 
CGCCGGACAG ACCCCATGGT GACATTGTCA TCCATTTTGG AGTCTATCAT CAATGACATG 
AGAGATCTTC CGAATACATA CCCTTTCCAC ACTCCAGTCA ATGCAAAGGT CGTAAAGGAC 
TACTACAAGA TCATCACCCG GCCAATGGAC TTACAGACAC TCCGTGAAAA TGTGCGTAAA 
CGCCTCTACC CATCTCGGGA AGAGTTCAGG GAGCATTTGG AGCTAATTGT GAAAAATAGT 
GCAACCTACA ATGGGCCAAA GCACTCACTG ACCCAGATCT CTCAATCAAT GCTGGATCTC 
TGTGATGAAA AACTTAAAGA GAAAGAAGAT AAATTGGCTC GTTTAGAGAA AGCAATCAAC 
CCCTTGCTGG ATGATGATGA CCAAGTGGCA TTTTCTTTTA TTCTGGACAA CATTGTCACC 
CAGAAAATGA TGGCAGTTCC AGATTCTTGG CCGTTTCATC ACCCAGTTAA TAAGAAGTTT 
GTTCCAGATT ATTACAAAGT AATTGTCAGT CCAATGGATT TAGAGACCAT ACGTAAAAAT 
ATCTCCAAGC ACAAGTATCA GAGTCGGGAG AGCTTTCTAG ACGATGTCAA CCTTATTCTG 
GCCAACAGTG TTAAATACAA TGGGCCTGAA AGTCAGTATA CTAAGACTGC TCAGGAGATT 
GTGAACGTCT GTTACCAGAC ACTGACTGAA TATGATGAGC ATTTAACTCA ACTTGAGAAG 
GATATTTGTA CAGCTAAAGA AGCAGCTTTG GAGGAAGCAG AATTAGAAAG CTTGGACCCA 
ATGACCCCAG GGCCTTACAC ACCTCAGCCT CCTGATTTGT ATGATACCAA CACATCCCTC 
AGTATGTCTC GAGATGCTTC TGTATTTCAA GATGAGAGCA ATATGTCTGT CCTGGATATT 
CCCACTGCCA CTCCAGAAAA GCAGGTGACA CAGATGCACC AGGGCCGAGG TAGGCTGGGC 
GAGGAAGACT CTGATGTAGA TATTGAAGGG TATGATGATG AGGAGGAGGA TGGGAAACCT 
AAGACACCAG CCCCAGAAGG TGAAGATGCA GATGGTGATC TTGCAGATGA AGAGGAAGGA 
ACTGTGCAAC AGCCTCAAGC CAGTGTCCTG TATGAGGATT TGCTTATGTC TGAAGGAGAA 
GATGATGAGG AAGATGCTGG GAGTGATGAA GAAGGAGATA ATCCTTTCTC TGCTATCCAG 
CTGAGTGAAA GTGGAAGTGA CTCGGATGTA GGATCTGGTG GGATAAGACC CAAACAGCCC 
CGCATGCTTC AAGAAAACAC AAGGATGGGC ATGGAAAATG AAGAAAGCAT GATGTCCTAT 
GAGGGAGACG GTGGGGAGGC TTCTCATGGT TTGGAGGATA GCAATATCAG TTATGGGAGC 
TATGAGGAGC CTGATCCCAA GTCGAACACC CAAGACACAA GCTTCAGCAG CATCGGTGGG 
TATGAGGTAT CAGAGGAGGA AGAAGATGAG GAGGAAGAGC AACGCTCTGG GCCGAGCGTA 
CTAAGCCAGG TCCAGCTGTC AGAGGACGAG GAGGACAGTG AGGATTTCCA CTCCATTGCT 
GGGGACAGTG ACTTGGACTC TGATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_002700041.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002700087.2 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X3, mRNA.

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CloneID	OBa13033
Clone ID Related Accession (Same CDS sequence)	XM_002700041.4 , XM_002700041.5 , XM_005200742.3
Accession Version	XM_002700041.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104728.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002700041.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG CCTCCTGATT TGTATGATAC CAACACATCC 
CTCAGTATGT CTCGAGATGC TTCTGTATTT CAAGATGAGA GCAATATGTC TGTCCTGGAT 
ATTCCCACTG CCACTCCAGA AAAGCAGGTG ACACAGGAAG GTGAAGATGC AGATGGTGAT 
CTTGCAGATG AAGAGGAAGG AACTGTGCAA CAGCCTCAAG CCAGTGTCCT GTATGAGGAT 
TTGCTTATGT CTGAAGGAGA AGATGATGAG GAAGATGCTG GGAGTGATGA AGAAGGAGAT 
AATCCTTTCT CTGCTATCCA GCTGAGTGAA AGTGGAAGTG ACTCGGATGT AGGATCTGGT 
GGGATAAGAC CCAAACAGCC CCGCATGCTT CAAGAAAACA CAAGGATGGG CATGGAAAAT 
GAAGAAAGCA TGATGTCCTA TGAGGGAGAC GGTGGGGAGG CTTCTCATGG TTTGGAGGAT 
AGCAATATCA GTTATGGGAG CTATGAGGAG CCTGATCCCA AGTCGAACAC CCAAGACACA 
AGCTTCAGCA GCATCGGTGG GTATGAGGTA TCAGAGGAGG AAGAAGATGA GGAGGAAGAG 
CAACGCTCTG GGCCGAGCGT ACTAAGCCAG GTCCAGCTGT CAGAGGACGA GGAGGACAGT 
GAGGATTTCC ACTCCATTGC TGGGGACAGT GACTTGGACT CTGATGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002700087.2
CDS	105..5735
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQEGEDADGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVQLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_002700041.4
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002700041.4

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG CCTCCTGATT TGTATGATAC CAACACATCC 
CTCAGTATGT CTCGAGATGC TTCTGTATTT CAAGATGAGA GCAATATGTC TGTCCTGGAT 
ATTCCCACTG CCACTCCAGA AAAGCAGGTG ACACAGGAAG GTGAAGATGC AGATGGTGAT 
CTTGCAGATG AAGAGGAAGG AACTGTGCAA CAGCCTCAAG CCAGTGTCCT GTATGAGGAT 
TTGCTTATGT CTGAAGGAGA AGATGATGAG GAAGATGCTG GGAGTGATGA AGAAGGAGAT 
AATCCTTTCT CTGCTATCCA GCTGAGTGAA AGTGGAAGTG ACTCGGATGT AGGATCTGGT 
GGGATAAGAC CCAAACAGCC CCGCATGCTT CAAGAAAACA CAAGGATGGG CATGGAAAAT 
GAAGAAAGCA TGATGTCCTA TGAGGGAGAC GGTGGGGAGG CTTCTCATGG TTTGGAGGAT 
AGCAATATCA GTTATGGGAG CTATGAGGAG CCTGATCCCA AGTCGAACAC CCAAGACACA 
AGCTTCAGCA GCATCGGTGG GTATGAGGTA TCAGAGGAGG AAGAAGATGA GGAGGAAGAG 
CAACGCTCTG GGCCGAGCGT ACTAAGCCAG GTCCAGCTGT CAGAGGACGA GGAGGACAGT 
GAGGATTTCC ACTCCATTGC TGGGGACAGT GACTTGGACT CTGATGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_002700041.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002700087.2 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X4, mRNA.

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CloneID	OBa13033
Clone ID Related Accession (Same CDS sequence)	XM_002700041.4 , XM_002700041.5 , XM_005200742.3
Accession Version	XM_002700041.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002700041.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG CCTCCTGATT TGTATGATAC CAACACATCC 
CTCAGTATGT CTCGAGATGC TTCTGTATTT CAAGATGAGA GCAATATGTC TGTCCTGGAT 
ATTCCCACTG CCACTCCAGA AAAGCAGGTG ACACAGGAAG GTGAAGATGC AGATGGTGAT 
CTTGCAGATG AAGAGGAAGG AACTGTGCAA CAGCCTCAAG CCAGTGTCCT GTATGAGGAT 
TTGCTTATGT CTGAAGGAGA AGATGATGAG GAAGATGCTG GGAGTGATGA AGAAGGAGAT 
AATCCTTTCT CTGCTATCCA GCTGAGTGAA AGTGGAAGTG ACTCGGATGT AGGATCTGGT 
GGGATAAGAC CCAAACAGCC CCGCATGCTT CAAGAAAACA CAAGGATGGG CATGGAAAAT 
GAAGAAAGCA TGATGTCCTA TGAGGGAGAC GGTGGGGAGG CTTCTCATGG TTTGGAGGAT 
AGCAATATCA GTTATGGGAG CTATGAGGAG CCTGATCCCA AGTCGAACAC CCAAGACACA 
AGCTTCAGCA GCATCGGTGG GTATGAGGTA TCAGAGGAGG AAGAAGATGA GGAGGAAGAG 
CAACGCTCTG GGCCGAGCGT ACTAAGCCAG GTCCAGCTGT CAGAGGACGA GGAGGACAGT 
GAGGATTTCC ACTCCATTGC TGGGGACAGT GACTTGGACT CTGATGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002700087.2
CDS	36..5666
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQEGEDADGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVQLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_002700041.5
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002700041.5

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG CCTCCTGATT TGTATGATAC CAACACATCC 
CTCAGTATGT CTCGAGATGC TTCTGTATTT CAAGATGAGA GCAATATGTC TGTCCTGGAT 
ATTCCCACTG CCACTCCAGA AAAGCAGGTG ACACAGGAAG GTGAAGATGC AGATGGTGAT 
CTTGCAGATG AAGAGGAAGG AACTGTGCAA CAGCCTCAAG CCAGTGTCCT GTATGAGGAT 
TTGCTTATGT CTGAAGGAGA AGATGATGAG GAAGATGCTG GGAGTGATGA AGAAGGAGAT 
AATCCTTTCT CTGCTATCCA GCTGAGTGAA AGTGGAAGTG ACTCGGATGT AGGATCTGGT 
GGGATAAGAC CCAAACAGCC CCGCATGCTT CAAGAAAACA CAAGGATGGG CATGGAAAAT 
GAAGAAAGCA TGATGTCCTA TGAGGGAGAC GGTGGGGAGG CTTCTCATGG TTTGGAGGAT 
AGCAATATCA GTTATGGGAG CTATGAGGAG CCTGATCCCA AGTCGAACAC CCAAGACACA 
AGCTTCAGCA GCATCGGTGG GTATGAGGTA TCAGAGGAGG AAGAAGATGA GGAGGAAGAG 
CAACGCTCTG GGCCGAGCGT ACTAAGCCAG GTCCAGCTGT CAGAGGACGA GGAGGACAGT 
GAGGATTTCC ACTCCATTGC TGGGGACAGT GACTTGGACT CTGATGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005200742.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005200799.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X3, mRNA.

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CloneID	OBa13033
Clone ID Related Accession (Same CDS sequence)	XM_002700041.4 , XM_002700041.5 , XM_005200742.3
Accession Version	XM_005200742.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647142.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005200742.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG CCTCCTGATT TGTATGATAC CAACACATCC 
CTCAGTATGT CTCGAGATGC TTCTGTATTT CAAGATGAGA GCAATATGTC TGTCCTGGAT 
ATTCCCACTG CCACTCCAGA AAAGCAGGTG ACACAGGAAG GTGAAGATGC AGATGGTGAT 
CTTGCAGATG AAGAGGAAGG AACTGTGCAA CAGCCTCAAG CCAGTGTCCT GTATGAGGAT 
TTGCTTATGT CTGAAGGAGA AGATGATGAG GAAGATGCTG GGAGTGATGA AGAAGGAGAT 
AATCCTTTCT CTGCTATCCA GCTGAGTGAA AGTGGAAGTG ACTCGGATGT AGGATCTGGT 
GGGATAAGAC CCAAACAGCC CCGCATGCTT CAAGAAAACA CAAGGATGGG CATGGAAAAT 
GAAGAAAGCA TGATGTCCTA TGAGGGAGAC GGTGGGGAGG CTTCTCATGG TTTGGAGGAT 
AGCAATATCA GTTATGGGAG CTATGAGGAG CCTGATCCCA AGTCGAACAC CCAAGACACA 
AGCTTCAGCA GCATCGGTGG GTATGAGGTA TCAGAGGAGG AAGAAGATGA GGAGGAAGAG 
CAACGCTCTG GGCCGAGCGT ACTAAGCCAG GTCCAGCTGT CAGAGGACGA GGAGGACAGT 
GAGGATTTCC ACTCCATTGC TGGGGACAGT GACTTGGACT CTGATGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005200799.1
CDS	105..5735
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQEGEDADGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVQLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_005200742.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005200742.3

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG CCTCCTGATT TGTATGATAC CAACACATCC 
CTCAGTATGT CTCGAGATGC TTCTGTATTT CAAGATGAGA GCAATATGTC TGTCCTGGAT 
ATTCCCACTG CCACTCCAGA AAAGCAGGTG ACACAGGAAG GTGAAGATGC AGATGGTGAT 
CTTGCAGATG AAGAGGAAGG AACTGTGCAA CAGCCTCAAG CCAGTGTCCT GTATGAGGAT 
TTGCTTATGT CTGAAGGAGA AGATGATGAG GAAGATGCTG GGAGTGATGA AGAAGGAGAT 
AATCCTTTCT CTGCTATCCA GCTGAGTGAA AGTGGAAGTG ACTCGGATGT AGGATCTGGT 
GGGATAAGAC CCAAACAGCC CCGCATGCTT CAAGAAAACA CAAGGATGGG CATGGAAAAT 
GAAGAAAGCA TGATGTCCTA TGAGGGAGAC GGTGGGGAGG CTTCTCATGG TTTGGAGGAT 
AGCAATATCA GTTATGGGAG CTATGAGGAG CCTGATCCCA AGTCGAACAC CCAAGACACA 
AGCTTCAGCA GCATCGGTGG GTATGAGGTA TCAGAGGAGG AAGAAGATGA GGAGGAAGAG 
CAACGCTCTG GGCCGAGCGT ACTAAGCCAG GTCCAGCTGT CAGAGGACGA GGAGGACAGT 
GAGGATTTCC ACTCCATTGC TGGGGACAGT GACTTGGACT CTGATGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005228050.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228107.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X7, mRNA.

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CloneID	OBa13035
Clone ID Related Accession (Same CDS sequence)	XM_005228050.4 , XM_005228050.3 , XM_005200744.3
Accession Version	XM_005228050.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228050.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGTTTTA TGCCATCACC TAACCACCAC AATCAAGGTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228107.1
CDS	68..4870
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNVLCHHLTTTIKV

Target ORF information:

RefSeq Version	XM_005228050.4
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228050.4

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGTTTTA TGCCATCACC TAACCACCAC AATCAAGGTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005228050.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228107.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X6, mRNA.

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CloneID	OBa13035
Clone ID Related Accession (Same CDS sequence)	XM_005228050.4 , XM_005228050.3 , XM_005200744.3
Accession Version	XM_005228050.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104728.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228050.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGTTTTA TGCCATCACC TAACCACCAC AATCAAGGTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228107.1
CDS	106..4908
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNVLCHHLTTTIKV

Target ORF information:

RefSeq Version	XM_005228050.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228050.3

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGTTTTA TGCCATCACC TAACCACCAC AATCAAGGTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005200744.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005200801.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa13035
Clone ID Related Accession (Same CDS sequence)	XM_005228050.4 , XM_005228050.3 , XM_005200744.3
Accession Version	XM_005200744.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647142.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005200744.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGTTTTA TGCCATCACC TAACCACCAC AATCAAGGTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005200801.1
CDS	106..4908
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNVLCHHLTTTIKV

Target ORF information:

RefSeq Version	XM_005200744.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005200744.3

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGTTTTA TGCCATCACC TAACCACCAC AATCAAGGTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005200745.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005200802.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa13036
Clone ID Related Accession (Same CDS sequence)	XM_005200745.3 , XM_005228051.3 , XM_005228051.4
Accession Version	XM_005200745.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647142.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005200745.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGTTTTC TGGTTACAGT GGGAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005200802.1
CDS	106..4896
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNVFWLQWED

Target ORF information:

RefSeq Version	XM_005200745.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005200745.3

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGTTTTC TGGTTACAGT GGGAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005228051.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228108.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X7, mRNA.

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CloneID	OBa13036
Clone ID Related Accession (Same CDS sequence)	XM_005200745.3 , XM_005228051.3 , XM_005228051.4
Accession Version	XM_005228051.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104728.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228051.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGTTTTC TGGTTACAGT GGGAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228108.1
CDS	106..4896
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNVFWLQWED

Target ORF information:

RefSeq Version	XM_005228051.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228051.3

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGTTTTC TGGTTACAGT GGGAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005228051.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228108.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X8, mRNA.

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CloneID	OBa13036
Clone ID Related Accession (Same CDS sequence)	XM_005200745.3 , XM_005228051.3 , XM_005228051.4
Accession Version	XM_005228051.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228051.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGTTTTC TGGTTACAGT GGGAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228108.1
CDS	68..4858
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNVFWLQWED

Target ORF information:

RefSeq Version	XM_005228051.4
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228051.4

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGTTTTC TGGTTACAGT GGGAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005228052.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228109.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X9, mRNA.

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CloneID	OBa13037
Clone ID Related Accession (Same CDS sequence)	XM_005228052.4 , XM_005228052.3 , XM_005200746.3
Accession Version	XM_005228052.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228052.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGTCTGG ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228109.1
CDS	68..4843
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNVWI

Target ORF information:

RefSeq Version	XM_005228052.4
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228052.4

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGTCTGG ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005228052.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228109.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X8, mRNA.

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CloneID	OBa13037
Clone ID Related Accession (Same CDS sequence)	XM_005228052.4 , XM_005228052.3 , XM_005200746.3
Accession Version	XM_005228052.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104728.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228052.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGTCTGG ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228109.1
CDS	106..4881
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNVWI

Target ORF information:

RefSeq Version	XM_005228052.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228052.3

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGTCTGG ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005200746.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005200803.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa13037
Clone ID Related Accession (Same CDS sequence)	XM_005228052.4 , XM_005228052.3 , XM_005200746.3
Accession Version	XM_005200746.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647142.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005200746.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGTCTGG ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005200803.1
CDS	106..4881
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNVWI

Target ORF information:

RefSeq Version	XM_005200746.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005200746.3

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGTCTGG ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_015470426.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325912.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa35503
Clone ID Related Accession (Same CDS sequence)	XM_015470426.1 , XM_005228048.4 , XM_005228048.3
Accession Version	XM_015470426.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647142.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG CCTCCTGATT TGTATGATAC CAACACATCC 
CTCAGTATGT CTCGAGATGC TTCTGTATTT CAAGATGAGA GCAATATGTC TGTCCTGGAT 
ATTCCCACTG CCACTCCAGA AAAGCAGGTG ACACAGATGC ACCAGGGCCG AGGTAGGCTG 
GGCGAGGAAG ACTCTGATGT AGATATTGAA GGGTATGATG ATGAGGAGGA GGATGGGAAA 
CCTAAGACAC CAGCCCCAGA AGGTGAAGAT GCAGATGGTG ATCTTGCAGA TGAAGAGGAA 
GGAACTGTGC AACAGCCTCA AGCCAGTGTC CTGTATGAGG ATTTGCTTAT GTCTGAAGGA 
GAAGATGATG AGGAAGATGC TGGGAGTGAT GAAGAAGGAG ATAATCCTTT CTCTGCTATC 
CAGCTGAGTG AAAGTGGAAG TGACTCGGAT GTAGGATCTG GTGGGATAAG ACCCAAACAG 
CCCCGCATGC TTCAAGAAAA CACAAGGATG GGCATGGAAA ATGAAGAAAG CATGATGTCC 
TATGAGGGAG ACGGTGGGGA GGCTTCTCAT GGTTTGGAGG ATAGCAATAT CAGTGGGAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325912.1
CDS	106..5628
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQMHQGRGRLGEEDSDVDIEGYDDEEEDGKPKTPAPEGEDADGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNISGS

Target ORF information:

RefSeq Version	XM_015470426.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470426.1

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG CCTCCTGATT TGTATGATAC CAACACATCC 
CTCAGTATGT CTCGAGATGC TTCTGTATTT CAAGATGAGA GCAATATGTC TGTCCTGGAT 
ATTCCCACTG CCACTCCAGA AAAGCAGGTG ACACAGATGC ACCAGGGCCG AGGTAGGCTG 
GGCGAGGAAG ACTCTGATGT AGATATTGAA GGGTATGATG ATGAGGAGGA GGATGGGAAA 
CCTAAGACAC CAGCCCCAGA AGGTGAAGAT GCAGATGGTG ATCTTGCAGA TGAAGAGGAA 
GGAACTGTGC AACAGCCTCA AGCCAGTGTC CTGTATGAGG ATTTGCTTAT GTCTGAAGGA 
GAAGATGATG AGGAAGATGC TGGGAGTGAT GAAGAAGGAG ATAATCCTTT CTCTGCTATC 
CAGCTGAGTG AAAGTGGAAG TGACTCGGAT GTAGGATCTG GTGGGATAAG ACCCAAACAG 
CCCCGCATGC TTCAAGAAAA CACAAGGATG GGCATGGAAA ATGAAGAAAG CATGATGTCC 
TATGAGGGAG ACGGTGGGGA GGCTTCTCAT GGTTTGGAGG ATAGCAATAT CAGTGGGAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005228048.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228105.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X6, mRNA.

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CloneID	OBa35503
Clone ID Related Accession (Same CDS sequence)	XM_015470426.1 , XM_005228048.4 , XM_005228048.3
Accession Version	XM_005228048.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228048.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG CCTCCTGATT TGTATGATAC CAACACATCC 
CTCAGTATGT CTCGAGATGC TTCTGTATTT CAAGATGAGA GCAATATGTC TGTCCTGGAT 
ATTCCCACTG CCACTCCAGA AAAGCAGGTG ACACAGATGC ACCAGGGCCG AGGTAGGCTG 
GGCGAGGAAG ACTCTGATGT AGATATTGAA GGGTATGATG ATGAGGAGGA GGATGGGAAA 
CCTAAGACAC CAGCCCCAGA AGGTGAAGAT GCAGATGGTG ATCTTGCAGA TGAAGAGGAA 
GGAACTGTGC AACAGCCTCA AGCCAGTGTC CTGTATGAGG ATTTGCTTAT GTCTGAAGGA 
GAAGATGATG AGGAAGATGC TGGGAGTGAT GAAGAAGGAG ATAATCCTTT CTCTGCTATC 
CAGCTGAGTG AAAGTGGAAG TGACTCGGAT GTAGGATCTG GTGGGATAAG ACCCAAACAG 
CCCCGCATGC TTCAAGAAAA CACAAGGATG GGCATGGAAA ATGAAGAAAG CATGATGTCC 
TATGAGGGAG ACGGTGGGGA GGCTTCTCAT GGTTTGGAGG ATAGCAATAT CAGTGGGAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228105.1
CDS	68..5590
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQMHQGRGRLGEEDSDVDIEGYDDEEEDGKPKTPAPEGEDADGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNISGS

Target ORF information:

RefSeq Version	XM_005228048.4
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228048.4

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG CCTCCTGATT TGTATGATAC CAACACATCC 
CTCAGTATGT CTCGAGATGC TTCTGTATTT CAAGATGAGA GCAATATGTC TGTCCTGGAT 
ATTCCCACTG CCACTCCAGA AAAGCAGGTG ACACAGATGC ACCAGGGCCG AGGTAGGCTG 
GGCGAGGAAG ACTCTGATGT AGATATTGAA GGGTATGATG ATGAGGAGGA GGATGGGAAA 
CCTAAGACAC CAGCCCCAGA AGGTGAAGAT GCAGATGGTG ATCTTGCAGA TGAAGAGGAA 
GGAACTGTGC AACAGCCTCA AGCCAGTGTC CTGTATGAGG ATTTGCTTAT GTCTGAAGGA 
GAAGATGATG AGGAAGATGC TGGGAGTGAT GAAGAAGGAG ATAATCCTTT CTCTGCTATC 
CAGCTGAGTG AAAGTGGAAG TGACTCGGAT GTAGGATCTG GTGGGATAAG ACCCAAACAG 
CCCCGCATGC TTCAAGAAAA CACAAGGATG GGCATGGAAA ATGAAGAAAG CATGATGTCC 
TATGAGGGAG ACGGTGGGGA GGCTTCTCAT GGTTTGGAGG ATAGCAATAT CAGTGGGAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005228048.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228105.1 Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X4, mRNA.

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CloneID	OBa35503
Clone ID Related Accession (Same CDS sequence)	XM_015470426.1 , XM_005228048.4 , XM_005228048.3
Accession Version	XM_005228048.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcription initiation factor TFIID subunit 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104728.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228048.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG CCTCCTGATT TGTATGATAC CAACACATCC 
CTCAGTATGT CTCGAGATGC TTCTGTATTT CAAGATGAGA GCAATATGTC TGTCCTGGAT 
ATTCCCACTG CCACTCCAGA AAAGCAGGTG ACACAGATGC ACCAGGGCCG AGGTAGGCTG 
GGCGAGGAAG ACTCTGATGT AGATATTGAA GGGTATGATG ATGAGGAGGA GGATGGGAAA 
CCTAAGACAC CAGCCCCAGA AGGTGAAGAT GCAGATGGTG ATCTTGCAGA TGAAGAGGAA 
GGAACTGTGC AACAGCCTCA AGCCAGTGTC CTGTATGAGG ATTTGCTTAT GTCTGAAGGA 
GAAGATGATG AGGAAGATGC TGGGAGTGAT GAAGAAGGAG ATAATCCTTT CTCTGCTATC 
CAGCTGAGTG AAAGTGGAAG TGACTCGGAT GTAGGATCTG GTGGGATAAG ACCCAAACAG 
CCCCGCATGC TTCAAGAAAA CACAAGGATG GGCATGGAAA ATGAAGAAAG CATGATGTCC 
TATGAGGGAG ACGGTGGGGA GGCTTCTCAT GGTTTGGAGG ATAGCAATAT CAGTGGGAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228105.1
CDS	106..5628
Translation	MSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDAALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHADYDEDDYDADCEDIDCKLMPPPPPPPGPVKKDKDQDAIAGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEAAQTESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQETECSVESEVNQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDTDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMEQEPVMKCRMADVSNTEYRIPEESSGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRFLEPPILTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMILAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQMHQGRGRLGEEDSDVDIEGYDDEEEDGKPKTPAPEGEDADGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNISGS

Target ORF information:

RefSeq Version	XM_005228048.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TATA-box binding protein associated factor 1 (TAF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228048.3

ORF Insert Sequence:

ATGTCAGACA CGGATAGCGA TGAAGATTCC GCTGGAGGCG GCCCATTTTC TTTAGCTGGT 
TTCCTTTTCG GCAACATCAA TGGAGCCGGG CAGTTGGAGG GAGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGGC TGGGCAGCCT GATAACTGAA 
CTCACAGCAA ATGAAGAACT GACTGGTACT GATGCTGCCC TGGTAAATGA TGAAGGATGG 
GTCAGGAGTA CAGAAGATGC TGTTGACTAT TCAGACATCA ACGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ACCAGCAGAC AATGGGGAGC TTGCAGCCTC TTTGCCATGC AGATTATGAT 
GAGGATGACT ATGATGCTGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAGTGAA AAAGGATAAG GACCAGGATG CAATTGCTGG TGTGTCTGAA 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCAGCACAG 
ACAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA GCACGATGCC 
ACCAAGCTGT TGCCGAGTGT CACGGAACTT TTCCCAGAAT TTCGTCCTGG GAAGGTGTTA 
CGCTTCCTAC GTCTTTTTGG ACCAGGAAAG AATGTCCCAT CTGTTTGGCG CAGTGCTCGG 
AGAAAGAGGA AGAAGAAACA CCGTGAACTG ATACAGGAAG AGCAGATCCA GGAGACGGAG 
TGCTCAGTAG AATCAGAAGT CAACCAGAAA TCTTTGTGGA ACTATGACTA TGCCCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATCACAA TGATGGCACC TGTGGAATCC 
AAGTTTTCCC AGTCAACTGG AGATACAGAT AAAGTGACAG ACACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ACTATGGCTT CAAACTAAGA AAGATGGAAC AGGAACCTGT GATGAAATGT 
AGAATGGCGG ATGTAAGCAA CACGGAATAC AGGATACCTG AGGAAAGCAG TGGCACCGAT 
CTTCTGGCTG ATGAAAACTT CCTGATGGTG ACACAGCTGC ATTGGGAAGA TGATATCATC 
TGGGATGGGG AGGATGTTAA ACACAAAGGG ACAAAACCAC AGCGTGCTAG CCTGGCAGGC 
TGGCTTCCTT CCAGCATGAC TAGGAATGCC ATGGCTTACA ATGTTCAACA AGGTTTTGCA 
GCAACGCTGG ATGATGACAA GCCTTGGTAC TCCATTTTTC CCATTGACAA TGAGGAGCTG 
GTATATGGAC GTTGGGAGGA CAATATCATT TGGGATGCTC AGGCCATGCC CCGGTTCTTG 
GAGCCTCCTA TTTTGACACT TGATCCCAAT GATGAGAACC TCATTTTGGA AATTCCAGAT 
GAGAAGGAAG AGGCTACTTC TAACTCTCCC TCCAAGGAGA GTAAGAAGGA ATCATCTCTG 
AAGAAGAGTC GAATTCTGTT AGGGAAAACA GGAGTCATCA AGGAGGAACC ACAGCAGAAC 
ATGTCTCAAC CAGAAGTGAA AGATCCATGG AATCTCTCCA ATGATGAATA TTACTACCCT 
AAGCAACAGG GTCTTCGAGG CACCTTTGGA GGAAATATTA TTCAGCATTC AATTCCTGCT 
GTGGAATTAC GGCAGCCCTT CTTTCCTACC CACATGGGGC CCATCAAACT CCGGCAGTTC 
CATCGCCCAC CTCTGAAGAA GTACTCTTTT GGGGCACTAT CTCAGCCAGG TCCCCACTCA 
GTCCAGCCCT TGCTAAAGCA CATCAAAAAG AAGGCAAAGA TGAGAGAACA AGAGAGGCAA 
GCTTCAGGTG GTGGAGAGAT GTTTTTCATG CGCACACCTC AGGACCTCAC AGGCAAAGAT 
GGAGATCTTA TTCTTGCAGA ATATAGTGAG GAAAACGGAC CATTAATGAT GCAAGTTGGC 
ATGGCAACCA AGATAAAAAA CTACTATAAG CGGAAACCCG GAAAAGATCC TGGAGCCCCA 
GATTGTAAAT ATGGAGAAAC TGTTTACTGC CATACATCTC CTTTTCTAGG CTCACTCCAT 
CCTGGACAAT TGCTGCAGGC ATTTGAGAAC AACCTTTTTC GTGCTCCAAT TTATCTTCAT 
AAGATGCCAG AAACTGACTT CCTTATTATT CGCACAAGAC AAGGTTACTA TATTCGGGAA 
TTAGTGGATA TTTTTGTGGT TGGCCAGCAG TGCCCTTTGT TTGAAGTTCC TGGGCCCAAC 
TCCAAAAGGG CCAATACTCA TATTCGAGAC TTTCTCCAGG TTTTTATTTA TCGCCTGTTC 
TGGAAGAGTA AAGATCGCCC ACGACGGATC CGAATGGAAG ATATAAAAAA AGCCTTTCCT 
TCTCACTCAG AAAGCAGCAT TCGGAAGAGG CTAAAGCTCT GCGCTGACTT CAAACGCACA 
GGCATGGATT CAAACTGGTG GGTTCTGAAG TCTGATTTTC GTTTACCAAC TGAAGAGGAG 
ATCAGGGCTA TGGTGTCTCC AGAGCAATGC TGTGCTTATT ACAGCATGAT AGCTGCAGAG 
CAGCGACTGA AGGATGCTGG CTATGGTGAG AAGTCCTTTT TTGCTCCAGA AGAGGAAAAT 
GAGGAAGATT TCCAGATGAA GATTGATGAT GAGGTTCGCA CTGCTCCTTG GAACACCACA 
CGGGCTTTCA TTGCTGCCAT GAAGGGCAAG TGTCTCCTGG AGGTGACTGG GGTGGCAGAT 
CCCACAGGGT GTGGTGAAGG ATTCTCCTAT GTGAAGATTC CAAACAAACC AACACAGCAG 
AAGGATGATA AGGAGCCTCA GCCAGTGAAG AAGACAGTGA CAGGAACAGA CGCAGACCTC 
CGTCGCCTTT CACTGAAAAA TGCCAAGCAA CTTCTCCGTA AATTTGGTGT GCCTGAGGAA 
GAGATTAAGA AGTTGTCCCG CTGGGAAGTG ATCGATGTAG TACGCACAAT GTCAACAGAA 
CAAGCCCGCT CTGGAGAGGG ACCCATGAGT AAATTTGCCC GTGGATCAAG GTTTTCTGTG 
GCTGAGCATC AAGAGCGTTA CAAAGAGGAA TGTCAGCGCA TCTTTGACCT GCAGAACAAG 
GTTTTATCAT CAACTGAAAT CTTATCAACT GACACAGACA GCAGCTCAGC TGAAGACAGT 
GACTTTGAGG AAATGGGAAA AAACATAGAG AACATGTTGC AGAATAAGAA AACCAGCTCT 
CAGCTGTCAC GTGAACGGGA GGAGCAGGAA CGGAAGGAAC TACAGAGGAT GATACTGGCA 
GCAGGCTCAG CAGCATCAGG AAACAATCAC AGAGATGATG ACACTGCTTC TGTGACAAGC 
CTTAATTCTT CTGCCACTGG CCGTTGTCTC AAGATTTATC GCACGTTTCG AGATGAGGAG 
GGGAAAGAAT ATGTTCGCTG TGAGACGGTC CGAAAGCCAG CTGTCATTGA TGCCTATGTA 
CGCATACGGA CCACAAAAGA CGAGGAATTC ATTCGAAAAT TTGCCCTTTT TGATGAACAA 
CATCGAGAAG AGATGCGAAA AGAACGGCGG AGGATTCAAG AGCAACTGAG ACGACTTAAG 
CGAAACCAGG AAAAGGAGAA GCTTAAGGGT CCTCCTGAGA AGAAGCCCAA AAAAATGAAG 
GAGCGTCCTG ACCTAAAACT GAAATGTGGG GCTTGTGGTG CCATTGGGCA CATGAGAACA 
AACAAATTCT GCCCTCTTTA TTATCAAACA AATGCTCCCC CTTCCAACCC TGTTGCCATG 
ACAGAGGAGC AGGAGGAGGA GTTGGAAAAG ACAGTCATTC ATAATGATAA TGAGGAACTT 
ATCAAGGTTG AAGGGACCAA GATTGTCCTA GGGAAACAGC TAATTGAGAG TGCAGATGAG 
GTTCGCAGAA AATCTCTGGT TCTCAAATTC CCTAAGCAGC AACTTCCTCC CAAGAAGAAA 
CGGCGAGTTG GAACTACTGT TCACTGTGAC TATCTGAATA GACCTCATAA GTCCATTCAC 
CGGCGCCGGA CAGACCCCAT GGTGACATTG TCATCCATTT TGGAGTCTAT CATCAATGAC 
ATGAGAGATC TTCCGAATAC ATACCCTTTC CACACTCCAG TCAATGCAAA GGTCGTAAAG 
GACTACTACA AGATCATCAC CCGGCCAATG GACTTACAGA CACTCCGTGA AAATGTGCGT 
AAACGCCTCT ACCCATCTCG GGAAGAGTTC AGGGAGCATT TGGAGCTAAT TGTGAAAAAT 
AGTGCAACCT ACAATGGGCC AAAGCACTCA CTGACCCAGA TCTCTCAATC AATGCTGGAT 
CTCTGTGATG AAAAACTTAA AGAGAAAGAA GATAAATTGG CTCGTTTAGA GAAAGCAATC 
AACCCCTTGC TGGATGATGA TGACCAAGTG GCATTTTCTT TTATTCTGGA CAACATTGTC 
ACCCAGAAAA TGATGGCAGT TCCAGATTCT TGGCCGTTTC ATCACCCAGT TAATAAGAAG 
TTTGTTCCAG ATTATTACAA AGTAATTGTC AGTCCAATGG ATTTAGAGAC CATACGTAAA 
AATATCTCCA AGCACAAGTA TCAGAGTCGG GAGAGCTTTC TAGACGATGT CAACCTTATT 
CTGGCCAACA GTGTTAAATA CAATGGGCCT GAAAGTCAGT ATACTAAGAC TGCTCAGGAG 
ATTGTGAACG TCTGTTACCA GACACTGACT GAATATGATG AGCATTTAAC TCAACTTGAG 
AAGGATATTT GTACAGCTAA AGAAGCAGCT TTGGAGGAAG CAGAATTAGA AAGCTTGGAC 
CCAATGACCC CAGGGCCTTA CACACCTCAG CCTCCTGATT TGTATGATAC CAACACATCC 
CTCAGTATGT CTCGAGATGC TTCTGTATTT CAAGATGAGA GCAATATGTC TGTCCTGGAT 
ATTCCCACTG CCACTCCAGA AAAGCAGGTG ACACAGATGC ACCAGGGCCG AGGTAGGCTG 
GGCGAGGAAG ACTCTGATGT AGATATTGAA GGGTATGATG ATGAGGAGGA GGATGGGAAA 
CCTAAGACAC CAGCCCCAGA AGGTGAAGAT GCAGATGGTG ATCTTGCAGA TGAAGAGGAA 
GGAACTGTGC AACAGCCTCA AGCCAGTGTC CTGTATGAGG ATTTGCTTAT GTCTGAAGGA 
GAAGATGATG AGGAAGATGC TGGGAGTGAT GAAGAAGGAG ATAATCCTTT CTCTGCTATC 
CAGCTGAGTG AAAGTGGAAG TGACTCGGAT GTAGGATCTG GTGGGATAAG ACCCAAACAG 
CCCCGCATGC TTCAAGAAAA CACAAGGATG GGCATGGAAA ATGAAGAAAG CATGATGTCC 
TATGAGGGAG ACGGTGGGGA GGCTTCTCAT GGTTTGGAGG ATAGCAATAT CAGTGGGAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TAF1
Entrez Gene ID	785085
Full Name	TATA-box binding protein associated factor 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003882	XP_308108.5
Xenopus tropicalis (tropical clawed frog)	taf1	XP_002934954.2
Canis lupus familiaris (dog)	TAF1	XP_005641531.1
Mus musculus (house mouse)	Taf1	NP_001074477.1
Danio rerio (zebrafish)	taf1	NP_001038250.1
Drosophila melanogaster (fruit fly)	Taf1	NP_996159.1
Caenorhabditis elegans (roundworm)	taf-1	NP_493426.2
Homo sapiens (human)	TAF1	NP_004597.2
Rattus norvegicus (Norway rat)	Taf1	NP_001178652.1
Bos taurus (cattle)	TAF1	XP_005228102.1
Gallus gallus (chicken)	TAF1	XP_420198.4
	TAF1	XP_002808585.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TAF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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