SEMA5B cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SEMA5B
Entrez Gene ID	784494
Full Name	semaphorin 5B
General protein information	Preferred Names semaphorin 5B Names semaphorin-5B sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002684809.4	XP_002684855.1	semaphorin-5B isoform X1
XM_001788280.4	XP_001788332.1	semaphorin-5B isoform X1
XM_010801202.2	XP_010799504.1	semaphorin-5B isoform X2
XM_010801202.3	XP_010799504.2	semaphorin-5B isoform X2
XM_010824635.2	XP_010822937.1	semaphorin-5B isoform X2
XM_002684809.5	XP_002684855.2	semaphorin-5B isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sema5b	NP_001100561.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002424	XP_312521.5
Canis lupus familiaris (dog)	SEMA5B	XP_005639706.1
Bos taurus (cattle)	SEMA5B	XP_002684855.1
Gallus gallus (chicken)	SEMA5B	XP_422096.2
Homo sapiens (human)	SEMA5B	NP_001026872.2
Pan troglodytes (chimpanzee)	SEMA5B	XP_516697.4
Mus musculus (house mouse)	Sema5b	NP_038689.2
	SEMA5B	XP_002802721.1
Danio rerio (zebrafish)	sema5ba	XP_684379.3
Drosophila melanogaster (fruit fly)	Sema-5c	NP_611293.3

Gene Ontology

The following SEMA5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa219135	XM_002684809.5 Latest version!	Bos taurus semaphorin 5B (SEMA5B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa219136	XM_010801202.3 Latest version!	Bos taurus semaphorin 5B (SEMA5B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa02539	XM_001788280.4 Latest version!	Bos taurus semaphorin 5B (SEMA5B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa02539	XM_002684809.4 Latest version!	Bos taurus semaphorin 5B (SEMA5B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa57547	XM_010824635.2 Latest version!	Bos taurus semaphorin 5B (SEMA5B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa57547	XM_010801202.2 Latest version!	Bos taurus semaphorin 5B (SEMA5B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEMA5B ( XM_002684809.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002684855.2 Bos taurus semaphorin 5B (SEMA5B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa219135
Clone ID Related Accession (Same CDS sequence)	XM_002684809.5
Accession Version	XM_002684809.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	semaphorin-5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002684809.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTCC CAGGACCCCT GGCTGTCACA CTGCTGCTAG TGCACCTCGC CAGCTCCCAG 
GATGTCGCCG GTGAAGCCAG CGGTGAGCAG CAGCTGTGCT CCCTGAGGGA ACACCCCATC 
GTGGCCTTTG CAGATCTGAA GCCATGGGTC TCTAACTTCA CTTACCCTGG AGCCCAAGAT 
TTCTCCCAGC TCGCTCTGGA TCCTTCCAGA AACCAGCTCA TCGTGGGAGC CAGGAACTAC 
CTCTTCAGAC TCAGCCTTGC CAATGTCTCC CTCCTTCAGG CCACAGAGTG GGCCTCCAAC 
GAGGACACGC GCCGCTCCTG CCAAAGCAAA GGGAAGACTG AGGAGGAGTG TCAGAACTAC 
GTGCGAGTCC TGATTGTCAC TGGCCGGAAA GTGTTCATGT GTGGGACCAA TGCCTTTTCC 
CCAGTGTGCT CCAGCAGACA GGTGGGGAAC CTCAGCCGGA CGGTGGAGAA GATCAATGGT 
GTGGCCCGCT GCCCCTATGA CCCACGCCAC AACTCCACTG CCGTCATCTC CTCTCAGGGG 
GAGCTCTATG CTGCCACAGT CATCGACTTC TCAGGTCGGG ACCCTGCTAT CTATCGCAGC 
CTGGGCAGCC GGCCACCACT TCGCACTGCC CAGTATAACT CCAAGTGGCT TAATGAGCCA 
AACTTTGTTG CAGCCTATGA TATCGGGCTG TTTGCATACT TCTTCCTGCG GGAGAACGCA 
GTGGAGCACG ACTGTGGACG CACCGTGTAC TCTCGGGTGG CCCGCGTGTG CAAGAACGAT 
GTGGGGGGCC GCTTCCTGCT GGAGGACACT TGGACCACGT TCATGAAGGC CCGGCTCAAC 
TGCTCCCGCC CGGGTGAGGT CCCCTTCTAC TACAACGAGC TGCAGAGCGC CTTCCATCTG 
CCCGAGCAGG ACCTCATCTA CGGGGTCTTC ACCACCAACG TAAACAGCAT CGCTGCGTCT 
GCTGTCTGCG CCTTCAATCT CAGTGCCATC TCCCAGGCTT TCAATGGCCC GTTTCGCTAC 
CAGGAGAACC CCAGGGCTGC CTGGCTCCCC ATCGCCAACC CCATCCCCAA TTTCCAGTGC 
GGCACCCTGC CGGAGGTGGG CCCCAATGAG AACCTGACGG AGCGCAGCCT GCAGGACGCA 
CAGCGCCTGT TCCTGATGAG CGAGGCTGTG CAGCCGGTGA CACCGGAGCC TTGCGTCACC 
CAAGACAGCG TGCGCTTCTC ACATCTCGTG GTGGACCTTG TGCAGGCCAA GGATACGCTC 
TACCACGTGC TCTACATCGG CACCGAGTCG GGCACCATCC TGAAGGCACT GTCCACGACG 
AGCCGCAGCC TCCGCGCTTG CTACCTGGAG GAGCTGCACG TGCTGCCCCC CGGGCGCCGG 
GAGCCACTGC GCAGCCTGCG CATCGTGCAC AGCGCCCGCG CGCTCTTCGT GGGGTTGAGC 
GACGGTGTGC TGCGCGTCCC GCTGGAGAGG TGCGCCGCCT ACCGCAGCCA GGGGGCCTGC 
CTGGGGGCAC GGGACCCGTA CTGCGGCTGG GATGGAAAGC AGCAACGTTG CAGCACTCTC 
GAGGACAGCT CCAACATGAG CCTCTGGACC CAGAACATCA CAGCATGTCC TGTGCGAAAT 
GTGACTCGGG ATGGGGGCTT CGGCCCATGG TCACCATGGC AACCATGTGA GCACTTGGAT 
GGGGACAACT CAGGCTCTTG CTTGTGTCGG TCCCGAGCCT GTGACTCCCC TCGACCCCGC 
TGTGGGGGCC TCGACTGCCT GGGGCCAGCC GTTCACATTG CCAACTGCTC CAGGAACGGG 
GCATGGACAC CGTGGTCGTC TTGGGCCCTG TGCAGCACCT CCTGTGGGAT CGGATTCCAG 
GTCCGCCAGC GAAGCTGCAG CAACCCCGCG CCACGCCACG GGGGCCGCAT CTGCGTGGGC 
AAGAGCCGGG AGGAGCGGTT CTGTAACGAA AACACGCCTT GCCCGGTGCC CATCTTCTGG 
GCATCCTGGG GCTCCTGGAG CAAGTGCAGC AGCAACTGTG GGGGCGGCGT GCAGTCGCGC 
CGCCGGACCT GTGAGAACGG CAACTCCTGC CCGGGCTGCG GCGTGGAGTT CAAGACATGC 
AATCCCGAGG CCTGCCCCGA AGTGCGGCGC AACACGCCGT GGACGCCGTG GCTGCCGGTG 
AACGTGACCC AGGGCGGGGC GCGGCAGGAG CAGCGCTTCC GCTTCACTTG CCGCGCGCCG 
CTGCCCGACC CCCACGGGCT GCAGTTTGGC CGAAGGAGGA TGGAAACCCG AACCTGCCCG 
GCGGACGGCT CAGGAGCCTG CGACACTGAC GCCCTGGTGG AGGATCTCCT GCGCAGCGGG 
GGCGCCTCCC CGCTCATGGT GAGCGGGGGC TGGGCCGCCT GGGGCCCATG GTCCTCCTGC 
TCCCGGGACT GTGAGCTGGG TTTCCGCTTC CGGAAGAGAA CATGCACGAA CCCCGAGCCC 
CGCAACGGGG GCCTGCCCTG CGTGGGTGAT GCCGCCGAGT ATCAGGACTG CAACCCGCAC 
GCTTGTCCAG TGCGGGGTGC CTGGTCTTGC TGGACCGCTT GGTCCCCGTG CTCTGCCTCC 
TGCGGTGGCG GCCACTACCA ACGCACCCGT TCCTGTACCA GCCCCGCGCC TTCCCCCGGC 
GAGGACATTT GTCTCGGGCT GCACACGGAG GAGGCGCTGT GTGCCACACA GGCCTGCCCA 
GAAGGCTGGT CGCCGTGGTC TGAGTGGAGT GCATGCACTG AGGATGGAGT GCAAAGCCGC 
GACAGGCACT GTGAGGAGCT GGTCCCTGGG CCCAGCGCTT GTGCTGGAAA CAGCAGCCAG 
AGCCGGCCCT GCCCTTACAG CGAGATTCCC GTGATCCTGC CTGCCTCCAG TGTGGATGAG 
GCCACCAGCT GTGGAGGGTT CAGTCTCATC CACCTGGTGG CCACGGGTAT CTCCTGCTTC 
CTGGGCTCTG GGCTGCTGAC CTTGGCGGTG TACCTGTCCT GCCAGCATTG CCAGCACCAG 
TCCCAGGAGT CCACGCTCGT CCATCCCGCC ACCCCCAACC ACCTGCACTA CAAGGGTGGG 
GGCACCCCCA AGAATGAGAA GTACACGCCC ATGGAATTCA AGACCCTGAA CAAGAATAAC 
CTGATCCCTG ATGACAGAGC CAACTTCTAC CCGTTGCAGC AGACCAACGT GTACACGACC 
ACCTACTACC CAAGCCCACT GAACAAACAC AGCTTCCGGC CTGAGGCCTC ACCTGGACAA 
CGGTGCTTCC CCAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002684855.2
CDS	401..3661
Translation	MVFPGPLAVTLLLVHLASSQDVAGEASGEQQLCSLREHPIVAFADLKPWVSNFTYPGAQDFSQLALDPSRNQLIVGARNYLFRLSLANVSLLQATEWASNEDTRRSCQSKGKTEEECQNYVRVLIVTGRKVFMCGTNAFSPVCSSRQVGNLSRTVEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSRPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPEVGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTTSRSLRACYLEELHVLPPGRREPLRSLRIVHSARALFVGLSDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLEDSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRSRACDSPRPRCGGLDCLGPAVHIANCSRNGAWTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGVQSRRRTCENGNSCPGCGVEFKTCNPEACPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLPDPHGLQFGRRRMETRTCPADGSGACDTDALVEDLLRSGGASPLMVSGGWAAWGPWSSCSRDCELGFRFRKRTCTNPEPRNGGLPCVGDAAEYQDCNPHACPVRGAWSCWTAWSPCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCATQACPEGWSPWSEWSACTEDGVQSRDRHCEELVPGPSACAGNSSQSRPCPYSEIPVILPASSVDEATSCGGFSLIHLVATGISCFLGSGLLTLAVYLSCQHCQHQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKHSFRPEASPGQRCFPNS

Target ORF information:

RefSeq Version	XM_002684809.5
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 5B (SEMA5B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002684809.5

ORF Insert Sequence:

ATGGTGTTCC CAGGACCCCT GGCTGTCACA CTGCTGCTAG TGCACCTCGC CAGCTCCCAG 
GATGTCGCCG GTGAAGCCAG CGGTGAGCAG CAGCTGTGCT CCCTGAGGGA ACACCCCATC 
GTGGCCTTTG CAGATCTGAA GCCATGGGTC TCTAACTTCA CTTACCCTGG AGCCCAAGAT 
TTCTCCCAGC TCGCTCTGGA TCCTTCCAGA AACCAGCTCA TCGTGGGAGC CAGGAACTAC 
CTCTTCAGAC TCAGCCTTGC CAATGTCTCC CTCCTTCAGG CCACAGAGTG GGCCTCCAAC 
GAGGACACGC GCCGCTCCTG CCAAAGCAAA GGGAAGACTG AGGAGGAGTG TCAGAACTAC 
GTGCGAGTCC TGATTGTCAC TGGCCGGAAA GTGTTCATGT GTGGGACCAA TGCCTTTTCC 
CCAGTGTGCT CCAGCAGACA GGTGGGGAAC CTCAGCCGGA CGGTGGAGAA GATCAATGGT 
GTGGCCCGCT GCCCCTATGA CCCACGCCAC AACTCCACTG CCGTCATCTC CTCTCAGGGG 
GAGCTCTATG CTGCCACAGT CATCGACTTC TCAGGTCGGG ACCCTGCTAT CTATCGCAGC 
CTGGGCAGCC GGCCACCACT TCGCACTGCC CAGTATAACT CCAAGTGGCT TAATGAGCCA 
AACTTTGTTG CAGCCTATGA TATCGGGCTG TTTGCATACT TCTTCCTGCG GGAGAACGCA 
GTGGAGCACG ACTGTGGACG CACCGTGTAC TCTCGGGTGG CCCGCGTGTG CAAGAACGAT 
GTGGGGGGCC GCTTCCTGCT GGAGGACACT TGGACCACGT TCATGAAGGC CCGGCTCAAC 
TGCTCCCGCC CGGGTGAGGT CCCCTTCTAC TACAACGAGC TGCAGAGCGC CTTCCATCTG 
CCCGAGCAGG ACCTCATCTA CGGGGTCTTC ACCACCAACG TAAACAGCAT CGCTGCGTCT 
GCTGTCTGCG CCTTCAATCT CAGTGCCATC TCCCAGGCTT TCAATGGCCC GTTTCGCTAC 
CAGGAGAACC CCAGGGCTGC CTGGCTCCCC ATCGCCAACC CCATCCCCAA TTTCCAGTGC 
GGCACCCTGC CGGAGGTGGG CCCCAATGAG AACCTGACGG AGCGCAGCCT GCAGGACGCA 
CAGCGCCTGT TCCTGATGAG CGAGGCTGTG CAGCCGGTGA CACCGGAGCC TTGCGTCACC 
CAAGACAGCG TGCGCTTCTC ACATCTCGTG GTGGACCTTG TGCAGGCCAA GGATACGCTC 
TACCACGTGC TCTACATCGG CACCGAGTCG GGCACCATCC TGAAGGCACT GTCCACGACG 
AGCCGCAGCC TCCGCGCTTG CTACCTGGAG GAGCTGCACG TGCTGCCCCC CGGGCGCCGG 
GAGCCACTGC GCAGCCTGCG CATCGTGCAC AGCGCCCGCG CGCTCTTCGT GGGGTTGAGC 
GACGGTGTGC TGCGCGTCCC GCTGGAGAGG TGCGCCGCCT ACCGCAGCCA GGGGGCCTGC 
CTGGGGGCAC GGGACCCGTA CTGCGGCTGG GATGGAAAGC AGCAACGTTG CAGCACTCTC 
GAGGACAGCT CCAACATGAG CCTCTGGACC CAGAACATCA CAGCATGTCC TGTGCGAAAT 
GTGACTCGGG ATGGGGGCTT CGGCCCATGG TCACCATGGC AACCATGTGA GCACTTGGAT 
GGGGACAACT CAGGCTCTTG CTTGTGTCGG TCCCGAGCCT GTGACTCCCC TCGACCCCGC 
TGTGGGGGCC TCGACTGCCT GGGGCCAGCC GTTCACATTG CCAACTGCTC CAGGAACGGG 
GCATGGACAC CGTGGTCGTC TTGGGCCCTG TGCAGCACCT CCTGTGGGAT CGGATTCCAG 
GTCCGCCAGC GAAGCTGCAG CAACCCCGCG CCACGCCACG GGGGCCGCAT CTGCGTGGGC 
AAGAGCCGGG AGGAGCGGTT CTGTAACGAA AACACGCCTT GCCCGGTGCC CATCTTCTGG 
GCATCCTGGG GCTCCTGGAG CAAGTGCAGC AGCAACTGTG GGGGCGGCGT GCAGTCGCGC 
CGCCGGACCT GTGAGAACGG CAACTCCTGC CCGGGCTGCG GCGTGGAGTT CAAGACATGC 
AATCCCGAGG CCTGCCCCGA AGTGCGGCGC AACACGCCGT GGACGCCGTG GCTGCCGGTG 
AACGTGACCC AGGGCGGGGC GCGGCAGGAG CAGCGCTTCC GCTTCACTTG CCGCGCGCCG 
CTGCCCGACC CCCACGGGCT GCAGTTTGGC CGAAGGAGGA TGGAAACCCG AACCTGCCCG 
GCGGACGGCT CAGGAGCCTG CGACACTGAC GCCCTGGTGG AGGATCTCCT GCGCAGCGGG 
GGCGCCTCCC CGCTCATGGT GAGCGGGGGC TGGGCCGCCT GGGGCCCATG GTCCTCCTGC 
TCCCGGGACT GTGAGCTGGG TTTCCGCTTC CGGAAGAGAA CATGCACGAA CCCCGAGCCC 
CGCAACGGGG GCCTGCCCTG CGTGGGTGAT GCCGCCGAGT ATCAGGACTG CAACCCGCAC 
GCTTGTCCAG TGCGGGGTGC CTGGTCTTGC TGGACCGCTT GGTCCCCGTG CTCTGCCTCC 
TGCGGTGGCG GCCACTACCA ACGCACCCGT TCCTGTACCA GCCCCGCGCC TTCCCCCGGC 
GAGGACATTT GTCTCGGGCT GCACACGGAG GAGGCGCTGT GTGCCACACA GGCCTGCCCA 
GAAGGCTGGT CGCCGTGGTC TGAGTGGAGT GCATGCACTG AGGATGGAGT GCAAAGCCGC 
GACAGGCACT GTGAGGAGCT GGTCCCTGGG CCCAGCGCTT GTGCTGGAAA CAGCAGCCAG 
AGCCGGCCCT GCCCTTACAG CGAGATTCCC GTGATCCTGC CTGCCTCCAG TGTGGATGAG 
GCCACCAGCT GTGGAGGGTT CAGTCTCATC CACCTGGTGG CCACGGGTAT CTCCTGCTTC 
CTGGGCTCTG GGCTGCTGAC CTTGGCGGTG TACCTGTCCT GCCAGCATTG CCAGCACCAG 
TCCCAGGAGT CCACGCTCGT CCATCCCGCC ACCCCCAACC ACCTGCACTA CAAGGGTGGG 
GGCACCCCCA AGAATGAGAA GTACACGCCC ATGGAATTCA AGACCCTGAA CAAGAATAAC 
CTGATCCCTG ATGACAGAGC CAACTTCTAC CCGTTGCAGC AGACCAACGT GTACACGACC 
ACCTACTACC CAAGCCCACT GAACAAACAC AGCTTCCGGC CTGAGGCCTC ACCTGGACAA 
CGGTGCTTCC CCAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5B ( XM_010801202.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799504.2 Bos taurus semaphorin 5B (SEMA5B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa219136
Clone ID Related Accession (Same CDS sequence)	XM_010801202.3
Accession Version	XM_010801202.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	semaphorin-5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801202.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAACT ACCTCTTCAG ACTCAGCCTT GCCAATGTCT CCCTCCTTCA GGCCACAGAG 
TGGGCCTCCA ACGAGGACAC GCGCCGCTCC TGCCAAAGCA AAGGGAAGAC TGAGGAGGAG 
TGTCAGAACT ACGTGCGAGT CCTGATTGTC ACTGGCCGGA AAGTGTTCAT GTGTGGGACC 
AATGCCTTTT CCCCAGTGTG CTCCAGCAGA CAGGTGGGGA ACCTCAGCCG GACGGTGGAG 
AAGATCAATG GTGTGGCCCG CTGCCCCTAT GACCCACGCC ACAACTCCAC TGCCGTCATC 
TCCTCTCAGG GGGAGCTCTA TGCTGCCACA GTCATCGACT TCTCAGGTCG GGACCCTGCT 
ATCTATCGCA GCCTGGGCAG CCGGCCACCA CTTCGCACTG CCCAGTATAA CTCCAAGTGG 
CTTAATGAGC CAAACTTTGT TGCAGCCTAT GATATCGGGC TGTTTGCATA CTTCTTCCTG 
CGGGAGAACG CAGTGGAGCA CGACTGTGGA CGCACCGTGT ACTCTCGGGT GGCCCGCGTG 
TGCAAGAACG ATGTGGGGGG CCGCTTCCTG CTGGAGGACA CTTGGACCAC GTTCATGAAG 
GCCCGGCTCA ACTGCTCCCG CCCGGGTGAG GTCCCCTTCT ACTACAACGA GCTGCAGAGC 
GCCTTCCATC TGCCCGAGCA GGACCTCATC TACGGGGTCT TCACCACCAA CGTAAACAGC 
ATCGCTGCGT CTGCTGTCTG CGCCTTCAAT CTCAGTGCCA TCTCCCAGGC TTTCAATGGC 
CCGTTTCGCT ACCAGGAGAA CCCCAGGGCT GCCTGGCTCC CCATCGCCAA CCCCATCCCC 
AATTTCCAGT GCGGCACCCT GCCGGAGGTG GGCCCCAATG AGAACCTGAC GGAGCGCAGC 
CTGCAGGACG CACAGCGCCT GTTCCTGATG AGCGAGGCTG TGCAGCCGGT GACACCGGAG 
CCTTGCGTCA CCCAAGACAG CGTGCGCTTC TCACATCTCG TGGTGGACCT TGTGCAGGCC 
AAGGATACGC TCTACCACGT GCTCTACATC GGCACCGAGT CGGGCACCAT CCTGAAGGCA 
CTGTCCACGA CGAGCCGCAG CCTCCGCGCT TGCTACCTGG AGGAGCTGCA CGTGCTGCCC 
CCCGGGCGCC GGGAGCCACT GCGCAGCCTG CGCATCGTGC ACAGCGCCCG CGCGCTCTTC 
GTGGGGTTGA GCGACGGTGT GCTGCGCGTC CCGCTGGAGA GGTGCGCCGC CTACCGCAGC 
CAGGGGGCCT GCCTGGGGGC ACGGGACCCG TACTGCGGCT GGGATGGAAA GCAGCAACGT 
TGCAGCACTC TCGAGGACAG CTCCAACATG AGCCTCTGGA CCCAGAACAT CACAGCATGT 
CCTGTGCGAA ATGTGACTCG GGATGGGGGC TTCGGCCCAT GGTCACCATG GCAACCATGT 
GAGCACTTGG ATGGGGACAA CTCAGGCTCT TGCTTGTGTC GGTCCCGAGC CTGTGACTCC 
CCTCGACCCC GCTGTGGGGG CCTCGACTGC CTGGGGCCAG CCGTTCACAT TGCCAACTGC 
TCCAGGAACG GGGCATGGAC ACCGTGGTCG TCTTGGGCCC TGTGCAGCAC CTCCTGTGGG 
ATCGGATTCC AGGTCCGCCA GCGAAGCTGC AGCAACCCCG CGCCACGCCA CGGGGGCCGC 
ATCTGCGTGG GCAAGAGCCG GGAGGAGCGG TTCTGTAACG AAAACACGCC TTGCCCGGTG 
CCCATCTTCT GGGCATCCTG GGGCTCCTGG AGCAAGTGCA GCAGCAACTG TGGGGGCGGC 
GTGCAGTCGC GCCGCCGGAC CTGTGAGAAC GGCAACTCCT GCCCGGGCTG CGGCGTGGAG 
TTCAAGACAT GCAATCCCGA GGCCTGCCCC GAAGTGCGGC GCAACACGCC GTGGACGCCG 
TGGCTGCCGG TGAACGTGAC CCAGGGCGGG GCGCGGCAGG AGCAGCGCTT CCGCTTCACT 
TGCCGCGCGC CGCTGCCCGA CCCCCACGGG CTGCAGTTTG GCCGAAGGAG GATGGAAACC 
CGAACCTGCC CGGCGGACGG CTCAGGAGCC TGCGACACTG ACGCCCTGGT GGAGGATCTC 
CTGCGCAGCG GGGGCGCCTC CCCGCTCATG GTGAGCGGGG GCTGGGCCGC CTGGGGCCCA 
TGGTCCTCCT GCTCCCGGGA CTGTGAGCTG GGTTTCCGCT TCCGGAAGAG AACATGCACG 
AACCCCGAGC CCCGCAACGG GGGCCTGCCC TGCGTGGGTG ATGCCGCCGA GTATCAGGAC 
TGCAACCCGC ACGCTTGTCC AGTGCGGGGT GCCTGGTCTT GCTGGACCGC TTGGTCCCCG 
TGCTCTGCCT CCTGCGGTGG CGGCCACTAC CAACGCACCC GTTCCTGTAC CAGCCCCGCG 
CCTTCCCCCG GCGAGGACAT TTGTCTCGGG CTGCACACGG AGGAGGCGCT GTGTGCCACA 
CAGGCCTGCC CAGAAGGCTG GTCGCCGTGG TCTGAGTGGA GTGCATGCAC TGAGGATGGA 
GTGCAAAGCC GCGACAGGCA CTGTGAGGAG CTGGTCCCTG GGCCCAGCGC TTGTGCTGGA 
AACAGCAGCC AGAGCCGGCC CTGCCCTTAC AGCGAGATTC CCGTGATCCT GCCTGCCTCC 
AGTGTGGATG AGGCCACCAG CTGTGGAGGG TTCAGTCTCA TCCACCTGGT GGCCACGGGT 
ATCTCCTGCT TCCTGGGCTC TGGGCTGCTG ACCTTGGCGG TGTACCTGTC CTGCCAGCAT 
TGCCAGCACC AGTCCCAGGA GTCCACGCTC GTCCATCCCG CCACCCCCAA CCACCTGCAC 
TACAAGGGTG GGGGCACCCC CAAGAATGAG AAGTACACGC CCATGGAATT CAAGACCCTG 
AACAAGAATA ACCTGATCCC TGATGACAGA GCCAACTTCT ACCCGTTGCA GCAGACCAAC 
GTGTACACGA CCACCTACTA CCCAAGCCCA CTGAACAAAC ACAGCTTCCG GCCTGAGGCC 
TCACCTGGAC AACGGTGCTT CCCCAACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799504.2
CDS	75..3107
Translation	MRNYLFRLSLANVSLLQATEWASNEDTRRSCQSKGKTEEECQNYVRVLIVTGRKVFMCGTNAFSPVCSSRQVGNLSRTVEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSRPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPEVGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTTSRSLRACYLEELHVLPPGRREPLRSLRIVHSARALFVGLSDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLEDSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRSRACDSPRPRCGGLDCLGPAVHIANCSRNGAWTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGVQSRRRTCENGNSCPGCGVEFKTCNPEACPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLPDPHGLQFGRRRMETRTCPADGSGACDTDALVEDLLRSGGASPLMVSGGWAAWGPWSSCSRDCELGFRFRKRTCTNPEPRNGGLPCVGDAAEYQDCNPHACPVRGAWSCWTAWSPCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCATQACPEGWSPWSEWSACTEDGVQSRDRHCEELVPGPSACAGNSSQSRPCPYSEIPVILPASSVDEATSCGGFSLIHLVATGISCFLGSGLLTLAVYLSCQHCQHQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKHSFRPEASPGQRCFPNS

Target ORF information:

RefSeq Version	XM_010801202.3
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 5B (SEMA5B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801202.3

ORF Insert Sequence:

ATGAGGAACT ACCTCTTCAG ACTCAGCCTT GCCAATGTCT CCCTCCTTCA GGCCACAGAG 
TGGGCCTCCA ACGAGGACAC GCGCCGCTCC TGCCAAAGCA AAGGGAAGAC TGAGGAGGAG 
TGTCAGAACT ACGTGCGAGT CCTGATTGTC ACTGGCCGGA AAGTGTTCAT GTGTGGGACC 
AATGCCTTTT CCCCAGTGTG CTCCAGCAGA CAGGTGGGGA ACCTCAGCCG GACGGTGGAG 
AAGATCAATG GTGTGGCCCG CTGCCCCTAT GACCCACGCC ACAACTCCAC TGCCGTCATC 
TCCTCTCAGG GGGAGCTCTA TGCTGCCACA GTCATCGACT TCTCAGGTCG GGACCCTGCT 
ATCTATCGCA GCCTGGGCAG CCGGCCACCA CTTCGCACTG CCCAGTATAA CTCCAAGTGG 
CTTAATGAGC CAAACTTTGT TGCAGCCTAT GATATCGGGC TGTTTGCATA CTTCTTCCTG 
CGGGAGAACG CAGTGGAGCA CGACTGTGGA CGCACCGTGT ACTCTCGGGT GGCCCGCGTG 
TGCAAGAACG ATGTGGGGGG CCGCTTCCTG CTGGAGGACA CTTGGACCAC GTTCATGAAG 
GCCCGGCTCA ACTGCTCCCG CCCGGGTGAG GTCCCCTTCT ACTACAACGA GCTGCAGAGC 
GCCTTCCATC TGCCCGAGCA GGACCTCATC TACGGGGTCT TCACCACCAA CGTAAACAGC 
ATCGCTGCGT CTGCTGTCTG CGCCTTCAAT CTCAGTGCCA TCTCCCAGGC TTTCAATGGC 
CCGTTTCGCT ACCAGGAGAA CCCCAGGGCT GCCTGGCTCC CCATCGCCAA CCCCATCCCC 
AATTTCCAGT GCGGCACCCT GCCGGAGGTG GGCCCCAATG AGAACCTGAC GGAGCGCAGC 
CTGCAGGACG CACAGCGCCT GTTCCTGATG AGCGAGGCTG TGCAGCCGGT GACACCGGAG 
CCTTGCGTCA CCCAAGACAG CGTGCGCTTC TCACATCTCG TGGTGGACCT TGTGCAGGCC 
AAGGATACGC TCTACCACGT GCTCTACATC GGCACCGAGT CGGGCACCAT CCTGAAGGCA 
CTGTCCACGA CGAGCCGCAG CCTCCGCGCT TGCTACCTGG AGGAGCTGCA CGTGCTGCCC 
CCCGGGCGCC GGGAGCCACT GCGCAGCCTG CGCATCGTGC ACAGCGCCCG CGCGCTCTTC 
GTGGGGTTGA GCGACGGTGT GCTGCGCGTC CCGCTGGAGA GGTGCGCCGC CTACCGCAGC 
CAGGGGGCCT GCCTGGGGGC ACGGGACCCG TACTGCGGCT GGGATGGAAA GCAGCAACGT 
TGCAGCACTC TCGAGGACAG CTCCAACATG AGCCTCTGGA CCCAGAACAT CACAGCATGT 
CCTGTGCGAA ATGTGACTCG GGATGGGGGC TTCGGCCCAT GGTCACCATG GCAACCATGT 
GAGCACTTGG ATGGGGACAA CTCAGGCTCT TGCTTGTGTC GGTCCCGAGC CTGTGACTCC 
CCTCGACCCC GCTGTGGGGG CCTCGACTGC CTGGGGCCAG CCGTTCACAT TGCCAACTGC 
TCCAGGAACG GGGCATGGAC ACCGTGGTCG TCTTGGGCCC TGTGCAGCAC CTCCTGTGGG 
ATCGGATTCC AGGTCCGCCA GCGAAGCTGC AGCAACCCCG CGCCACGCCA CGGGGGCCGC 
ATCTGCGTGG GCAAGAGCCG GGAGGAGCGG TTCTGTAACG AAAACACGCC TTGCCCGGTG 
CCCATCTTCT GGGCATCCTG GGGCTCCTGG AGCAAGTGCA GCAGCAACTG TGGGGGCGGC 
GTGCAGTCGC GCCGCCGGAC CTGTGAGAAC GGCAACTCCT GCCCGGGCTG CGGCGTGGAG 
TTCAAGACAT GCAATCCCGA GGCCTGCCCC GAAGTGCGGC GCAACACGCC GTGGACGCCG 
TGGCTGCCGG TGAACGTGAC CCAGGGCGGG GCGCGGCAGG AGCAGCGCTT CCGCTTCACT 
TGCCGCGCGC CGCTGCCCGA CCCCCACGGG CTGCAGTTTG GCCGAAGGAG GATGGAAACC 
CGAACCTGCC CGGCGGACGG CTCAGGAGCC TGCGACACTG ACGCCCTGGT GGAGGATCTC 
CTGCGCAGCG GGGGCGCCTC CCCGCTCATG GTGAGCGGGG GCTGGGCCGC CTGGGGCCCA 
TGGTCCTCCT GCTCCCGGGA CTGTGAGCTG GGTTTCCGCT TCCGGAAGAG AACATGCACG 
AACCCCGAGC CCCGCAACGG GGGCCTGCCC TGCGTGGGTG ATGCCGCCGA GTATCAGGAC 
TGCAACCCGC ACGCTTGTCC AGTGCGGGGT GCCTGGTCTT GCTGGACCGC TTGGTCCCCG 
TGCTCTGCCT CCTGCGGTGG CGGCCACTAC CAACGCACCC GTTCCTGTAC CAGCCCCGCG 
CCTTCCCCCG GCGAGGACAT TTGTCTCGGG CTGCACACGG AGGAGGCGCT GTGTGCCACA 
CAGGCCTGCC CAGAAGGCTG GTCGCCGTGG TCTGAGTGGA GTGCATGCAC TGAGGATGGA 
GTGCAAAGCC GCGACAGGCA CTGTGAGGAG CTGGTCCCTG GGCCCAGCGC TTGTGCTGGA 
AACAGCAGCC AGAGCCGGCC CTGCCCTTAC AGCGAGATTC CCGTGATCCT GCCTGCCTCC 
AGTGTGGATG AGGCCACCAG CTGTGGAGGG TTCAGTCTCA TCCACCTGGT GGCCACGGGT 
ATCTCCTGCT TCCTGGGCTC TGGGCTGCTG ACCTTGGCGG TGTACCTGTC CTGCCAGCAT 
TGCCAGCACC AGTCCCAGGA GTCCACGCTC GTCCATCCCG CCACCCCCAA CCACCTGCAC 
TACAAGGGTG GGGGCACCCC CAAGAATGAG AAGTACACGC CCATGGAATT CAAGACCCTG 
AACAAGAATA ACCTGATCCC TGATGACAGA GCCAACTTCT ACCCGTTGCA GCAGACCAAC 
GTGTACACGA CCACCTACTA CCCAAGCCCA CTGAACAAAC ACAGCTTCCG GCCTGAGGCC 
TCACCTGGAC AACGGTGCTT CCCCAACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5B ( XM_002684809.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002684855.1 Bos taurus semaphorin 5B (SEMA5B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa02539
Clone ID Related Accession (Same CDS sequence)	XM_002684809.4 , XM_001788280.4
Accession Version	XM_002684809.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	semaphorin-5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103811.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002684809.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTCC CAGGACCCCT GGCTGTCACA CTGCTGCTAG TGCACCTCGC CAGCTCCCAG 
GATGTCGCCG GTGAAGCCAG CGGTGAGCAG CAGCTGTGCT CCCTGAGGGA ACACCCCATC 
GTGGCCTTTG CAGATCTGAA GCCATGGGTC TCTAACTTCA CTTACCCTGG AGCCCAAGAT 
TTCTCCCAGC TCGCTCTGGA TCCTTCCAGA AACCAGCTCA TCGTGGGAGC CAGGAACTAC 
CTCTTCAGAC TCAGCCTTGC CAATGTCTCC CTCCTTCAGG CCACAGAGTG GGCCTCCAAC 
GAGGACACGC GCCGCTCCTG CCAAAGCAAA GGGAAGACTG AGGAGGAGTG TCAGAACTAC 
GTGCGAGTCC TGATTGTCAC TGGCCGGAAA GTGTTCATGT GTGGGACCAA TGCCTTTTCC 
CCAGTGTGCT CCAGCAGACA GGTGGGGAAC CTCAGCCGGA CGGTGGAGAA GATCAATGGT 
GTGGCCCGCT GCCCCTATGA CCCACGCCAC AACTCCACTG CCGTCATCTC CTCTCAGGGG 
GAGCTCTATG CTGCCACAGT CATCGACTTC TCAGGTCGGG ACCCTGCTAT CTATCGCAGC 
CTGGGCAGCC GGCCACCACT TCGCACTGCC CAGTATAACT CCAAGTGGCT TAATGAGCCA 
AACTTTGTTG CAGCCTATGA TATCGGGCTG TTTGCATACT TCTTCCTGCG GGAGAACGCA 
GTGGAGCACG ACTGTGGACG CACCGTGTAC TCTCGGGTGG CCCGCGTGTG CAAGAACGAT 
GTGGGGGGCC GCTTCCTGCT GGAGGACACT TGGACCACGT TCATGAAGGC CCGGCTCAAC 
TGCTCCCGCC CGGGTGAGGT CCCCTTCTAC TACAACGAGC TGCAGAGCGC CTTCCATCTG 
CCCGAGCAGG ACCTCATCTA CGGGGTCTTC ACCACCAACG TAAACAGCAT CGCTGCGTCT 
GCTGTCTGCG CCTTCAATCT CAGTGCCATC TCCCAGGCTT TCAATGGCCC GTTTCGCTAC 
CAGGAGAACC CCAGGGCTGC CTGGCTCCCC ATCGCCAACC CCATCCCCAA TTTCCAGTGC 
GGCACCCTGC CGGAGGTGGG CCCCAATGAG AACCTGACGG AGCGCAGCCT GCAGGACGCA 
CAGCGCCTGT TCCTGATGAG CGAGGCTGTG CAGCCGGTGA CACCGGAGCC TTGCGTCACC 
CAAGACAGCG TGCGCTTCTC ACATCTCGTG GTGGACCTTG TGCAGGCCAA GGATACGCTC 
TACCACGTGC TCTACATCGG CACCGAGTCG GGCACCATCC TGAAGGCACT GTCCACGACG 
AGCCGCAGCC TCCGCGGTTG CTACCTGGAG GAGCTGCACG TGCTGCCCCC CGGGCGCCGG 
GAGCCACTGC GCAGCCTGCG CATCGTGCAC AGCGCCCGCG CGCTCTTCGT GGGGTTGAGC 
GACGGTGTGC TGCGCGTCCC GCTGGAGAGG TGCGCCGCCT ACCGCAGCCA GGGGGCCTGC 
CTGGGGGCAC GGGACCCGTA CTGCGGCTGG GATGGAAAGC AGCAACGTTG CAGCACTCTC 
GAGGACAGCT CCAACATGAG CCTCTGGACC CAGAACATCA CAGCATGTCC TGTGCGAAAT 
GTGACTCGGG ATGGGGGCTT CGGCCCATGG TCACCATGGC AACCATGTGA GCACTTGGAT 
GGGGACAACT CAGGCTCTTG CTTGTGTCGG TCCCGAGCCT GTGACTCCCC TCGACCCCGC 
TGTGGGGGCC TCGACTGCCT GGGGCCAGCC GTTCACATTG CCAACTGCTC CAGGAACGGG 
GCATGGACAC CGTGGTCGTC TTGGGCCCTG TGCAGCACCT CCTGTGGGAT CGGATTCCAG 
GTCCGCCAGC GAAGCTGCAG CAACCCCGCG CCACGCCACG GGGGCCGCAT CTGCGTGGGC 
AAGAGCCGGG AGGAGCGGTT CTGTAACGAA AACACGCCTT GCCCGGTGCC CATCTTCTGG 
GCATCCTGGG GCTCCTGGAG CAAGTGCAGC AGCAACTGTG GGGGCGGCGT GCAGTCGCGC 
CGCCGGACCT GTGAGAACGG CAACTCCTGC CCGGGCTGCG GCGTGGAGTT CAAGACATGC 
AATCCCGAGG CCTGCCCCGA AGTGCGGCGC AACACGCCGT GGACGCCGTG GCTGCCGGTG 
AACGTGACCC AGGGCGGGGC GCGGCAGGAG CAGCGCTTCC GCTTCACTTG CCGCGCGCCG 
CTGCCCGACC CCCACGGGCT GCAGTTTGGC CGAAGGAGGA TGGAAACCCG AACCTGCCCG 
GCGGACGGCT CAGGAGCCTG CGACACTGAC GCCCTGGTGG AGGATCTCCT GCGCAGCGGG 
GGCGCCTCCC CGCTCATGGT GAGCGGGGGC TGGGCCGCCT GGGGCCCATG GTCCTCCTGC 
TCCCGGGACT GTGAGCTGGG TTTCCGCTTC CGGAAGAGAA CATGCACGAA CCCCGAGCCC 
CGCAACGGGG GCCTGCCCTG CGTGGGTGAT GCCGCCGAGT ATCAGGACTG CAACCCGCAC 
GCTTGTCCAG TGCGGGGTGC CTGGTCTTGC TGGACCGCTT GGTCCCCGTG CTCTGCCTCC 
TGCGGTGGCG GCCACTACCA ACGCACCCGT TCCTGTACCA GCCCCGCGCC TTCCCCCGGC 
GAGGACATTT GTCTCGGGCT GCACACGGAG GAGGCGCTGT GTGCCACACA GGCCTGCCCA 
GAAGGCTGGT CGCCGTGGTC TGAGTGGAGT GCATGCACTG AGGATGGAGT GCAAAGCCGC 
GACAGGCACT GTGAGGAGCT GGTCCCTGGG CCCAGCGCTT GTGCTGGAAA CAGCAGCCAG 
AGCCGGCCCT GCCCTTACAG CGAGATTCCC GTGATCCTGC CTGCCTCCAG TGTGGATGAG 
GCCACCAGCT GTGGAGGGTT CAGTCTCATC CACCTGGTGG CCACGGGTAT CTCCTGCTTC 
CTGGGCTCTG GGCTGCTGAC CTTGGCGGTG TACCTGTCCT GCCAGCATTG CCAGCACCAG 
TCCCAGGAGT CCACGCTCGT CCATCCCGCC ACCCCCAACC ACCTGCACTA CAAGGGTGGG 
GGCACCCCCA AGAATGAGAA GTACATGCCC ATGGAATTCA AGACCCTGAA CAAGAATAAC 
CTGATCCCTG ATGACAGAGC CAACTTCTAC CCGTTGCAGC AGACCAACGT GTACACGACC 
ACCTACTACC CAAGCCCACT GAACAAACAC AGCTTCCGGC CTGAGGCCTC ACCTGGACAA 
CGGTGCTTCC CCAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002684855.1
CDS	479..3739
Translation	MVFPGPLAVTLLLVHLASSQDVAGEASGEQQLCSLREHPIVAFADLKPWVSNFTYPGAQDFSQLALDPSRNQLIVGARNYLFRLSLANVSLLQATEWASNEDTRRSCQSKGKTEEECQNYVRVLIVTGRKVFMCGTNAFSPVCSSRQVGNLSRTVEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSRPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPEVGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTTSRSLRGCYLEELHVLPPGRREPLRSLRIVHSARALFVGLSDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLEDSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRSRACDSPRPRCGGLDCLGPAVHIANCSRNGAWTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGVQSRRRTCENGNSCPGCGVEFKTCNPEACPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLPDPHGLQFGRRRMETRTCPADGSGACDTDALVEDLLRSGGASPLMVSGGWAAWGPWSSCSRDCELGFRFRKRTCTNPEPRNGGLPCVGDAAEYQDCNPHACPVRGAWSCWTAWSPCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCATQACPEGWSPWSEWSACTEDGVQSRDRHCEELVPGPSACAGNSSQSRPCPYSEIPVILPASSVDEATSCGGFSLIHLVATGISCFLGSGLLTLAVYLSCQHCQHQSQESTLVHPATPNHLHYKGGGTPKNEKYMPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKHSFRPEASPGQRCFPNS

Target ORF information:

RefSeq Version	XM_002684809.4
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 5B (SEMA5B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002684809.4

ORF Insert Sequence:

ATGGTGTTCC CAGGACCCCT GGCTGTCACA CTGCTGCTAG TGCACCTCGC CAGCTCCCAG 
GATGTCGCCG GTGAAGCCAG CGGTGAGCAG CAGCTGTGCT CCCTGAGGGA ACACCCCATC 
GTGGCCTTTG CAGATCTGAA GCCATGGGTC TCTAACTTCA CTTACCCTGG AGCCCAAGAT 
TTCTCCCAGC TCGCTCTGGA TCCTTCCAGA AACCAGCTCA TCGTGGGAGC CAGGAACTAC 
CTCTTCAGAC TCAGCCTTGC CAATGTCTCC CTCCTTCAGG CCACAGAGTG GGCCTCCAAC 
GAGGACACGC GCCGCTCCTG CCAAAGCAAA GGGAAGACTG AGGAGGAGTG TCAGAACTAC 
GTGCGAGTCC TGATTGTCAC TGGCCGGAAA GTGTTCATGT GTGGGACCAA TGCCTTTTCC 
CCAGTGTGCT CCAGCAGACA GGTGGGGAAC CTCAGCCGGA CGGTGGAGAA GATCAATGGT 
GTGGCCCGCT GCCCCTATGA CCCACGCCAC AACTCCACTG CCGTCATCTC CTCTCAGGGG 
GAGCTCTATG CTGCCACAGT CATCGACTTC TCAGGTCGGG ACCCTGCTAT CTATCGCAGC 
CTGGGCAGCC GGCCACCACT TCGCACTGCC CAGTATAACT CCAAGTGGCT TAATGAGCCA 
AACTTTGTTG CAGCCTATGA TATCGGGCTG TTTGCATACT TCTTCCTGCG GGAGAACGCA 
GTGGAGCACG ACTGTGGACG CACCGTGTAC TCTCGGGTGG CCCGCGTGTG CAAGAACGAT 
GTGGGGGGCC GCTTCCTGCT GGAGGACACT TGGACCACGT TCATGAAGGC CCGGCTCAAC 
TGCTCCCGCC CGGGTGAGGT CCCCTTCTAC TACAACGAGC TGCAGAGCGC CTTCCATCTG 
CCCGAGCAGG ACCTCATCTA CGGGGTCTTC ACCACCAACG TAAACAGCAT CGCTGCGTCT 
GCTGTCTGCG CCTTCAATCT CAGTGCCATC TCCCAGGCTT TCAATGGCCC GTTTCGCTAC 
CAGGAGAACC CCAGGGCTGC CTGGCTCCCC ATCGCCAACC CCATCCCCAA TTTCCAGTGC 
GGCACCCTGC CGGAGGTGGG CCCCAATGAG AACCTGACGG AGCGCAGCCT GCAGGACGCA 
CAGCGCCTGT TCCTGATGAG CGAGGCTGTG CAGCCGGTGA CACCGGAGCC TTGCGTCACC 
CAAGACAGCG TGCGCTTCTC ACATCTCGTG GTGGACCTTG TGCAGGCCAA GGATACGCTC 
TACCACGTGC TCTACATCGG CACCGAGTCG GGCACCATCC TGAAGGCACT GTCCACGACG 
AGCCGCAGCC TCCGCGGTTG CTACCTGGAG GAGCTGCACG TGCTGCCCCC CGGGCGCCGG 
GAGCCACTGC GCAGCCTGCG CATCGTGCAC AGCGCCCGCG CGCTCTTCGT GGGGTTGAGC 
GACGGTGTGC TGCGCGTCCC GCTGGAGAGG TGCGCCGCCT ACCGCAGCCA GGGGGCCTGC 
CTGGGGGCAC GGGACCCGTA CTGCGGCTGG GATGGAAAGC AGCAACGTTG CAGCACTCTC 
GAGGACAGCT CCAACATGAG CCTCTGGACC CAGAACATCA CAGCATGTCC TGTGCGAAAT 
GTGACTCGGG ATGGGGGCTT CGGCCCATGG TCACCATGGC AACCATGTGA GCACTTGGAT 
GGGGACAACT CAGGCTCTTG CTTGTGTCGG TCCCGAGCCT GTGACTCCCC TCGACCCCGC 
TGTGGGGGCC TCGACTGCCT GGGGCCAGCC GTTCACATTG CCAACTGCTC CAGGAACGGG 
GCATGGACAC CGTGGTCGTC TTGGGCCCTG TGCAGCACCT CCTGTGGGAT CGGATTCCAG 
GTCCGCCAGC GAAGCTGCAG CAACCCCGCG CCACGCCACG GGGGCCGCAT CTGCGTGGGC 
AAGAGCCGGG AGGAGCGGTT CTGTAACGAA AACACGCCTT GCCCGGTGCC CATCTTCTGG 
GCATCCTGGG GCTCCTGGAG CAAGTGCAGC AGCAACTGTG GGGGCGGCGT GCAGTCGCGC 
CGCCGGACCT GTGAGAACGG CAACTCCTGC CCGGGCTGCG GCGTGGAGTT CAAGACATGC 
AATCCCGAGG CCTGCCCCGA AGTGCGGCGC AACACGCCGT GGACGCCGTG GCTGCCGGTG 
AACGTGACCC AGGGCGGGGC GCGGCAGGAG CAGCGCTTCC GCTTCACTTG CCGCGCGCCG 
CTGCCCGACC CCCACGGGCT GCAGTTTGGC CGAAGGAGGA TGGAAACCCG AACCTGCCCG 
GCGGACGGCT CAGGAGCCTG CGACACTGAC GCCCTGGTGG AGGATCTCCT GCGCAGCGGG 
GGCGCCTCCC CGCTCATGGT GAGCGGGGGC TGGGCCGCCT GGGGCCCATG GTCCTCCTGC 
TCCCGGGACT GTGAGCTGGG TTTCCGCTTC CGGAAGAGAA CATGCACGAA CCCCGAGCCC 
CGCAACGGGG GCCTGCCCTG CGTGGGTGAT GCCGCCGAGT ATCAGGACTG CAACCCGCAC 
GCTTGTCCAG TGCGGGGTGC CTGGTCTTGC TGGACCGCTT GGTCCCCGTG CTCTGCCTCC 
TGCGGTGGCG GCCACTACCA ACGCACCCGT TCCTGTACCA GCCCCGCGCC TTCCCCCGGC 
GAGGACATTT GTCTCGGGCT GCACACGGAG GAGGCGCTGT GTGCCACACA GGCCTGCCCA 
GAAGGCTGGT CGCCGTGGTC TGAGTGGAGT GCATGCACTG AGGATGGAGT GCAAAGCCGC 
GACAGGCACT GTGAGGAGCT GGTCCCTGGG CCCAGCGCTT GTGCTGGAAA CAGCAGCCAG 
AGCCGGCCCT GCCCTTACAG CGAGATTCCC GTGATCCTGC CTGCCTCCAG TGTGGATGAG 
GCCACCAGCT GTGGAGGGTT CAGTCTCATC CACCTGGTGG CCACGGGTAT CTCCTGCTTC 
CTGGGCTCTG GGCTGCTGAC CTTGGCGGTG TACCTGTCCT GCCAGCATTG CCAGCACCAG 
TCCCAGGAGT CCACGCTCGT CCATCCCGCC ACCCCCAACC ACCTGCACTA CAAGGGTGGG 
GGCACCCCCA AGAATGAGAA GTACATGCCC ATGGAATTCA AGACCCTGAA CAAGAATAAC 
CTGATCCCTG ATGACAGAGC CAACTTCTAC CCGTTGCAGC AGACCAACGT GTACACGACC 
ACCTACTACC CAAGCCCACT GAACAAACAC AGCTTCCGGC CTGAGGCCTC ACCTGGACAA 
CGGTGCTTCC CCAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5B ( XM_001788280.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_001788332.1 Bos taurus semaphorin 5B (SEMA5B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa02539
Clone ID Related Accession (Same CDS sequence)	XM_002684809.4 , XM_001788280.4
Accession Version	XM_001788280.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	semaphorin-5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644520.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001788280.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTCC CAGGACCCCT GGCTGTCACA CTGCTGCTAG TGCACCTCGC CAGCTCCCAG 
GATGTCGCCG GTGAAGCCAG CGGTGAGCAG CAGCTGTGCT CCCTGAGGGA ACACCCCATC 
GTGGCCTTTG CAGATCTGAA GCCATGGGTC TCTAACTTCA CTTACCCTGG AGCCCAAGAT 
TTCTCCCAGC TCGCTCTGGA TCCTTCCAGA AACCAGCTCA TCGTGGGAGC CAGGAACTAC 
CTCTTCAGAC TCAGCCTTGC CAATGTCTCC CTCCTTCAGG CCACAGAGTG GGCCTCCAAC 
GAGGACACGC GCCGCTCCTG CCAAAGCAAA GGGAAGACTG AGGAGGAGTG TCAGAACTAC 
GTGCGAGTCC TGATTGTCAC TGGCCGGAAA GTGTTCATGT GTGGGACCAA TGCCTTTTCC 
CCAGTGTGCT CCAGCAGACA GGTGGGGAAC CTCAGCCGGA CGGTGGAGAA GATCAATGGT 
GTGGCCCGCT GCCCCTATGA CCCACGCCAC AACTCCACTG CCGTCATCTC CTCTCAGGGG 
GAGCTCTATG CTGCCACAGT CATCGACTTC TCAGGTCGGG ACCCTGCTAT CTATCGCAGC 
CTGGGCAGCC GGCCACCACT TCGCACTGCC CAGTATAACT CCAAGTGGCT TAATGAGCCA 
AACTTTGTTG CAGCCTATGA TATCGGGCTG TTTGCATACT TCTTCCTGCG GGAGAACGCA 
GTGGAGCACG ACTGTGGACG CACCGTGTAC TCTCGGGTGG CCCGCGTGTG CAAGAACGAT 
GTGGGGGGCC GCTTCCTGCT GGAGGACACT TGGACCACGT TCATGAAGGC CCGGCTCAAC 
TGCTCCCGCC CGGGTGAGGT CCCCTTCTAC TACAACGAGC TGCAGAGCGC CTTCCATCTG 
CCCGAGCAGG ACCTCATCTA CGGGGTCTTC ACCACCAACG TAAACAGCAT CGCTGCGTCT 
GCTGTCTGCG CCTTCAATCT CAGTGCCATC TCCCAGGCTT TCAATGGCCC GTTTCGCTAC 
CAGGAGAACC CCAGGGCTGC CTGGCTCCCC ATCGCCAACC CCATCCCCAA TTTCCAGTGC 
GGCACCCTGC CGGAGGTGGG CCCCAATGAG AACCTGACGG AGCGCAGCCT GCAGGACGCA 
CAGCGCCTGT TCCTGATGAG CGAGGCTGTG CAGCCGGTGA CACCGGAGCC TTGCGTCACC 
CAAGACAGCG TGCGCTTCTC ACATCTCGTG GTGGACCTTG TGCAGGCCAA GGATACGCTC 
TACCACGTGC TCTACATCGG CACCGAGTCG GGCACCATCC TGAAGGCACT GTCCACGACG 
AGCCGCAGCC TCCGCGGTTG CTACCTGGAG GAGCTGCACG TGCTGCCCCC CGGGCGCCGG 
GAGCCACTGC GCAGCCTGCG CATCGTGCAC AGCGCCCGCG CGCTCTTCGT GGGGTTGAGC 
GACGGTGTGC TGCGCGTCCC GCTGGAGAGG TGCGCCGCCT ACCGCAGCCA GGGGGCCTGC 
CTGGGGGCAC GGGACCCGTA CTGCGGCTGG GATGGAAAGC AGCAACGTTG CAGCACTCTC 
GAGGACAGCT CCAACATGAG CCTCTGGACC CAGAACATCA CAGCATGTCC TGTGCGAAAT 
GTGACTCGGG ATGGGGGCTT CGGCCCATGG TCACCATGGC AACCATGTGA GCACTTGGAT 
GGGGACAACT CAGGCTCTTG CTTGTGTCGG TCCCGAGCCT GTGACTCCCC TCGACCCCGC 
TGTGGGGGCC TCGACTGCCT GGGGCCAGCC GTTCACATTG CCAACTGCTC CAGGAACGGG 
GCATGGACAC CGTGGTCGTC TTGGGCCCTG TGCAGCACCT CCTGTGGGAT CGGATTCCAG 
GTCCGCCAGC GAAGCTGCAG CAACCCCGCG CCACGCCACG GGGGCCGCAT CTGCGTGGGC 
AAGAGCCGGG AGGAGCGGTT CTGTAACGAA AACACGCCTT GCCCGGTGCC CATCTTCTGG 
GCATCCTGGG GCTCCTGGAG CAAGTGCAGC AGCAACTGTG GGGGCGGCGT GCAGTCGCGC 
CGCCGGACCT GTGAGAACGG CAACTCCTGC CCGGGCTGCG GCGTGGAGTT CAAGACATGC 
AATCCCGAGG CCTGCCCCGA AGTGCGGCGC AACACGCCGT GGACGCCGTG GCTGCCGGTG 
AACGTGACCC AGGGCGGGGC GCGGCAGGAG CAGCGCTTCC GCTTCACTTG CCGCGCGCCG 
CTGCCCGACC CCCACGGGCT GCAGTTTGGC CGAAGGAGGA TGGAAACCCG AACCTGCCCG 
GCGGACGGCT CAGGAGCCTG CGACACTGAC GCCCTGGTGG AGGATCTCCT GCGCAGCGGG 
GGCGCCTCCC CGCTCATGGT GAGCGGGGGC TGGGCCGCCT GGGGCCCATG GTCCTCCTGC 
TCCCGGGACT GTGAGCTGGG TTTCCGCTTC CGGAAGAGAA CATGCACGAA CCCCGAGCCC 
CGCAACGGGG GCCTGCCCTG CGTGGGTGAT GCCGCCGAGT ATCAGGACTG CAACCCGCAC 
GCTTGTCCAG TGCGGGGTGC CTGGTCTTGC TGGACCGCTT GGTCCCCGTG CTCTGCCTCC 
TGCGGTGGCG GCCACTACCA ACGCACCCGT TCCTGTACCA GCCCCGCGCC TTCCCCCGGC 
GAGGACATTT GTCTCGGGCT GCACACGGAG GAGGCGCTGT GTGCCACACA GGCCTGCCCA 
GAAGGCTGGT CGCCGTGGTC TGAGTGGAGT GCATGCACTG AGGATGGAGT GCAAAGCCGC 
GACAGGCACT GTGAGGAGCT GGTCCCTGGG CCCAGCGCTT GTGCTGGAAA CAGCAGCCAG 
AGCCGGCCCT GCCCTTACAG CGAGATTCCC GTGATCCTGC CTGCCTCCAG TGTGGATGAG 
GCCACCAGCT GTGGAGGGTT CAGTCTCATC CACCTGGTGG CCACGGGTAT CTCCTGCTTC 
CTGGGCTCTG GGCTGCTGAC CTTGGCGGTG TACCTGTCCT GCCAGCATTG CCAGCACCAG 
TCCCAGGAGT CCACGCTCGT CCATCCCGCC ACCCCCAACC ACCTGCACTA CAAGGGTGGG 
GGCACCCCCA AGAATGAGAA GTACATGCCC ATGGAATTCA AGACCCTGAA CAAGAATAAC 
CTGATCCCTG ATGACAGAGC CAACTTCTAC CCGTTGCAGC AGACCAACGT GTACACGACC 
ACCTACTACC CAAGCCCACT GAACAAACAC AGCTTCCGGC CTGAGGCCTC ACCTGGACAA 
CGGTGCTTCC CCAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001788332.1
CDS	479..3739
Translation	MVFPGPLAVTLLLVHLASSQDVAGEASGEQQLCSLREHPIVAFADLKPWVSNFTYPGAQDFSQLALDPSRNQLIVGARNYLFRLSLANVSLLQATEWASNEDTRRSCQSKGKTEEECQNYVRVLIVTGRKVFMCGTNAFSPVCSSRQVGNLSRTVEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSRPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPEVGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTTSRSLRGCYLEELHVLPPGRREPLRSLRIVHSARALFVGLSDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLEDSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRSRACDSPRPRCGGLDCLGPAVHIANCSRNGAWTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGVQSRRRTCENGNSCPGCGVEFKTCNPEACPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLPDPHGLQFGRRRMETRTCPADGSGACDTDALVEDLLRSGGASPLMVSGGWAAWGPWSSCSRDCELGFRFRKRTCTNPEPRNGGLPCVGDAAEYQDCNPHACPVRGAWSCWTAWSPCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCATQACPEGWSPWSEWSACTEDGVQSRDRHCEELVPGPSACAGNSSQSRPCPYSEIPVILPASSVDEATSCGGFSLIHLVATGISCFLGSGLLTLAVYLSCQHCQHQSQESTLVHPATPNHLHYKGGGTPKNEKYMPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKHSFRPEASPGQRCFPNS

Target ORF information:

RefSeq Version	XM_001788280.4
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 5B (SEMA5B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001788280.4

ORF Insert Sequence:

ATGGTGTTCC CAGGACCCCT GGCTGTCACA CTGCTGCTAG TGCACCTCGC CAGCTCCCAG 
GATGTCGCCG GTGAAGCCAG CGGTGAGCAG CAGCTGTGCT CCCTGAGGGA ACACCCCATC 
GTGGCCTTTG CAGATCTGAA GCCATGGGTC TCTAACTTCA CTTACCCTGG AGCCCAAGAT 
TTCTCCCAGC TCGCTCTGGA TCCTTCCAGA AACCAGCTCA TCGTGGGAGC CAGGAACTAC 
CTCTTCAGAC TCAGCCTTGC CAATGTCTCC CTCCTTCAGG CCACAGAGTG GGCCTCCAAC 
GAGGACACGC GCCGCTCCTG CCAAAGCAAA GGGAAGACTG AGGAGGAGTG TCAGAACTAC 
GTGCGAGTCC TGATTGTCAC TGGCCGGAAA GTGTTCATGT GTGGGACCAA TGCCTTTTCC 
CCAGTGTGCT CCAGCAGACA GGTGGGGAAC CTCAGCCGGA CGGTGGAGAA GATCAATGGT 
GTGGCCCGCT GCCCCTATGA CCCACGCCAC AACTCCACTG CCGTCATCTC CTCTCAGGGG 
GAGCTCTATG CTGCCACAGT CATCGACTTC TCAGGTCGGG ACCCTGCTAT CTATCGCAGC 
CTGGGCAGCC GGCCACCACT TCGCACTGCC CAGTATAACT CCAAGTGGCT TAATGAGCCA 
AACTTTGTTG CAGCCTATGA TATCGGGCTG TTTGCATACT TCTTCCTGCG GGAGAACGCA 
GTGGAGCACG ACTGTGGACG CACCGTGTAC TCTCGGGTGG CCCGCGTGTG CAAGAACGAT 
GTGGGGGGCC GCTTCCTGCT GGAGGACACT TGGACCACGT TCATGAAGGC CCGGCTCAAC 
TGCTCCCGCC CGGGTGAGGT CCCCTTCTAC TACAACGAGC TGCAGAGCGC CTTCCATCTG 
CCCGAGCAGG ACCTCATCTA CGGGGTCTTC ACCACCAACG TAAACAGCAT CGCTGCGTCT 
GCTGTCTGCG CCTTCAATCT CAGTGCCATC TCCCAGGCTT TCAATGGCCC GTTTCGCTAC 
CAGGAGAACC CCAGGGCTGC CTGGCTCCCC ATCGCCAACC CCATCCCCAA TTTCCAGTGC 
GGCACCCTGC CGGAGGTGGG CCCCAATGAG AACCTGACGG AGCGCAGCCT GCAGGACGCA 
CAGCGCCTGT TCCTGATGAG CGAGGCTGTG CAGCCGGTGA CACCGGAGCC TTGCGTCACC 
CAAGACAGCG TGCGCTTCTC ACATCTCGTG GTGGACCTTG TGCAGGCCAA GGATACGCTC 
TACCACGTGC TCTACATCGG CACCGAGTCG GGCACCATCC TGAAGGCACT GTCCACGACG 
AGCCGCAGCC TCCGCGGTTG CTACCTGGAG GAGCTGCACG TGCTGCCCCC CGGGCGCCGG 
GAGCCACTGC GCAGCCTGCG CATCGTGCAC AGCGCCCGCG CGCTCTTCGT GGGGTTGAGC 
GACGGTGTGC TGCGCGTCCC GCTGGAGAGG TGCGCCGCCT ACCGCAGCCA GGGGGCCTGC 
CTGGGGGCAC GGGACCCGTA CTGCGGCTGG GATGGAAAGC AGCAACGTTG CAGCACTCTC 
GAGGACAGCT CCAACATGAG CCTCTGGACC CAGAACATCA CAGCATGTCC TGTGCGAAAT 
GTGACTCGGG ATGGGGGCTT CGGCCCATGG TCACCATGGC AACCATGTGA GCACTTGGAT 
GGGGACAACT CAGGCTCTTG CTTGTGTCGG TCCCGAGCCT GTGACTCCCC TCGACCCCGC 
TGTGGGGGCC TCGACTGCCT GGGGCCAGCC GTTCACATTG CCAACTGCTC CAGGAACGGG 
GCATGGACAC CGTGGTCGTC TTGGGCCCTG TGCAGCACCT CCTGTGGGAT CGGATTCCAG 
GTCCGCCAGC GAAGCTGCAG CAACCCCGCG CCACGCCACG GGGGCCGCAT CTGCGTGGGC 
AAGAGCCGGG AGGAGCGGTT CTGTAACGAA AACACGCCTT GCCCGGTGCC CATCTTCTGG 
GCATCCTGGG GCTCCTGGAG CAAGTGCAGC AGCAACTGTG GGGGCGGCGT GCAGTCGCGC 
CGCCGGACCT GTGAGAACGG CAACTCCTGC CCGGGCTGCG GCGTGGAGTT CAAGACATGC 
AATCCCGAGG CCTGCCCCGA AGTGCGGCGC AACACGCCGT GGACGCCGTG GCTGCCGGTG 
AACGTGACCC AGGGCGGGGC GCGGCAGGAG CAGCGCTTCC GCTTCACTTG CCGCGCGCCG 
CTGCCCGACC CCCACGGGCT GCAGTTTGGC CGAAGGAGGA TGGAAACCCG AACCTGCCCG 
GCGGACGGCT CAGGAGCCTG CGACACTGAC GCCCTGGTGG AGGATCTCCT GCGCAGCGGG 
GGCGCCTCCC CGCTCATGGT GAGCGGGGGC TGGGCCGCCT GGGGCCCATG GTCCTCCTGC 
TCCCGGGACT GTGAGCTGGG TTTCCGCTTC CGGAAGAGAA CATGCACGAA CCCCGAGCCC 
CGCAACGGGG GCCTGCCCTG CGTGGGTGAT GCCGCCGAGT ATCAGGACTG CAACCCGCAC 
GCTTGTCCAG TGCGGGGTGC CTGGTCTTGC TGGACCGCTT GGTCCCCGTG CTCTGCCTCC 
TGCGGTGGCG GCCACTACCA ACGCACCCGT TCCTGTACCA GCCCCGCGCC TTCCCCCGGC 
GAGGACATTT GTCTCGGGCT GCACACGGAG GAGGCGCTGT GTGCCACACA GGCCTGCCCA 
GAAGGCTGGT CGCCGTGGTC TGAGTGGAGT GCATGCACTG AGGATGGAGT GCAAAGCCGC 
GACAGGCACT GTGAGGAGCT GGTCCCTGGG CCCAGCGCTT GTGCTGGAAA CAGCAGCCAG 
AGCCGGCCCT GCCCTTACAG CGAGATTCCC GTGATCCTGC CTGCCTCCAG TGTGGATGAG 
GCCACCAGCT GTGGAGGGTT CAGTCTCATC CACCTGGTGG CCACGGGTAT CTCCTGCTTC 
CTGGGCTCTG GGCTGCTGAC CTTGGCGGTG TACCTGTCCT GCCAGCATTG CCAGCACCAG 
TCCCAGGAGT CCACGCTCGT CCATCCCGCC ACCCCCAACC ACCTGCACTA CAAGGGTGGG 
GGCACCCCCA AGAATGAGAA GTACATGCCC ATGGAATTCA AGACCCTGAA CAAGAATAAC 
CTGATCCCTG ATGACAGAGC CAACTTCTAC CCGTTGCAGC AGACCAACGT GTACACGACC 
ACCTACTACC CAAGCCCACT GAACAAACAC AGCTTCCGGC CTGAGGCCTC ACCTGGACAA 
CGGTGCTTCC CCAACAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5B ( XM_010801202.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799504.1 Bos taurus semaphorin 5B (SEMA5B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa57547
Clone ID Related Accession (Same CDS sequence)	XM_010801202.2 , XM_010824635.2
Accession Version	XM_010801202.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	semaphorin-5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103811.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801202.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAACT ACCTCTTCAG ACTCAGCCTT GCCAATGTCT CCCTCCTTCA GGCCACAGAG 
TGGGCCTCCA ACGAGGACAC GCGCCGCTCC TGCCAAAGCA AAGGGAAGAC TGAGGAGGAG 
TGTCAGAACT ACGTGCGAGT CCTGATTGTC ACTGGCCGGA AAGTGTTCAT GTGTGGGACC 
AATGCCTTTT CCCCAGTGTG CTCCAGCAGA CAGGTGGGGA ACCTCAGCCG GACGGTGGAG 
AAGATCAATG GTGTGGCCCG CTGCCCCTAT GACCCACGCC ACAACTCCAC TGCCGTCATC 
TCCTCTCAGG GGGAGCTCTA TGCTGCCACA GTCATCGACT TCTCAGGTCG GGACCCTGCT 
ATCTATCGCA GCCTGGGCAG CCGGCCACCA CTTCGCACTG CCCAGTATAA CTCCAAGTGG 
CTTAATGAGC CAAACTTTGT TGCAGCCTAT GATATCGGGC TGTTTGCATA CTTCTTCCTG 
CGGGAGAACG CAGTGGAGCA CGACTGTGGA CGCACCGTGT ACTCTCGGGT GGCCCGCGTG 
TGCAAGAACG ATGTGGGGGG CCGCTTCCTG CTGGAGGACA CTTGGACCAC GTTCATGAAG 
GCCCGGCTCA ACTGCTCCCG CCCGGGTGAG GTCCCCTTCT ACTACAACGA GCTGCAGAGC 
GCCTTCCATC TGCCCGAGCA GGACCTCATC TACGGGGTCT TCACCACCAA CGTAAACAGC 
ATCGCTGCGT CTGCTGTCTG CGCCTTCAAT CTCAGTGCCA TCTCCCAGGC TTTCAATGGC 
CCGTTTCGCT ACCAGGAGAA CCCCAGGGCT GCCTGGCTCC CCATCGCCAA CCCCATCCCC 
AATTTCCAGT GCGGCACCCT GCCGGAGGTG GGCCCCAATG AGAACCTGAC GGAGCGCAGC 
CTGCAGGACG CACAGCGCCT GTTCCTGATG AGCGAGGCTG TGCAGCCGGT GACACCGGAG 
CCTTGCGTCA CCCAAGACAG CGTGCGCTTC TCACATCTCG TGGTGGACCT TGTGCAGGCC 
AAGGATACGC TCTACCACGT GCTCTACATC GGCACCGAGT CGGGCACCAT CCTGAAGGCA 
CTGTCCACGA CGAGCCGCAG CCTCCGCGGT TGCTACCTGG AGGAGCTGCA CGTGCTGCCC 
CCCGGGCGCC GGGAGCCACT GCGCAGCCTG CGCATCGTGC ACAGCGCCCG CGCGCTCTTC 
GTGGGGTTGA GCGACGGTGT GCTGCGCGTC CCGCTGGAGA GGTGCGCCGC CTACCGCAGC 
CAGGGGGCCT GCCTGGGGGC ACGGGACCCG TACTGCGGCT GGGATGGAAA GCAGCAACGT 
TGCAGCACTC TCGAGGACAG CTCCAACATG AGCCTCTGGA CCCAGAACAT CACAGCATGT 
CCTGTGCGAA ATGTGACTCG GGATGGGGGC TTCGGCCCAT GGTCACCATG GCAACCATGT 
GAGCACTTGG ATGGGGACAA CTCAGGCTCT TGCTTGTGTC GGTCCCGAGC CTGTGACTCC 
CCTCGACCCC GCTGTGGGGG CCTCGACTGC CTGGGGCCAG CCGTTCACAT TGCCAACTGC 
TCCAGGAACG GGGCATGGAC ACCGTGGTCG TCTTGGGCCC TGTGCAGCAC CTCCTGTGGG 
ATCGGATTCC AGGTCCGCCA GCGAAGCTGC AGCAACCCCG CGCCACGCCA CGGGGGCCGC 
ATCTGCGTGG GCAAGAGCCG GGAGGAGCGG TTCTGTAACG AAAACACGCC TTGCCCGGTG 
CCCATCTTCT GGGCATCCTG GGGCTCCTGG AGCAAGTGCA GCAGCAACTG TGGGGGCGGC 
GTGCAGTCGC GCCGCCGGAC CTGTGAGAAC GGCAACTCCT GCCCGGGCTG CGGCGTGGAG 
TTCAAGACAT GCAATCCCGA GGCCTGCCCC GAAGTGCGGC GCAACACGCC GTGGACGCCG 
TGGCTGCCGG TGAACGTGAC CCAGGGCGGG GCGCGGCAGG AGCAGCGCTT CCGCTTCACT 
TGCCGCGCGC CGCTGCCCGA CCCCCACGGG CTGCAGTTTG GCCGAAGGAG GATGGAAACC 
CGAACCTGCC CGGCGGACGG CTCAGGAGCC TGCGACACTG ACGCCCTGGT GGAGGATCTC 
CTGCGCAGCG GGGGCGCCTC CCCGCTCATG GTGAGCGGGG GCTGGGCCGC CTGGGGCCCA 
TGGTCCTCCT GCTCCCGGGA CTGTGAGCTG GGTTTCCGCT TCCGGAAGAG AACATGCACG 
AACCCCGAGC CCCGCAACGG GGGCCTGCCC TGCGTGGGTG ATGCCGCCGA GTATCAGGAC 
TGCAACCCGC ACGCTTGTCC AGTGCGGGGT GCCTGGTCTT GCTGGACCGC TTGGTCCCCG 
TGCTCTGCCT CCTGCGGTGG CGGCCACTAC CAACGCACCC GTTCCTGTAC CAGCCCCGCG 
CCTTCCCCCG GCGAGGACAT TTGTCTCGGG CTGCACACGG AGGAGGCGCT GTGTGCCACA 
CAGGCCTGCC CAGAAGGCTG GTCGCCGTGG TCTGAGTGGA GTGCATGCAC TGAGGATGGA 
GTGCAAAGCC GCGACAGGCA CTGTGAGGAG CTGGTCCCTG GGCCCAGCGC TTGTGCTGGA 
AACAGCAGCC AGAGCCGGCC CTGCCCTTAC AGCGAGATTC CCGTGATCCT GCCTGCCTCC 
AGTGTGGATG AGGCCACCAG CTGTGGAGGG TTCAGTCTCA TCCACCTGGT GGCCACGGGT 
ATCTCCTGCT TCCTGGGCTC TGGGCTGCTG ACCTTGGCGG TGTACCTGTC CTGCCAGCAT 
TGCCAGCACC AGTCCCAGGA GTCCACGCTC GTCCATCCCG CCACCCCCAA CCACCTGCAC 
TACAAGGGTG GGGGCACCCC CAAGAATGAG AAGTACATGC CCATGGAATT CAAGACCCTG 
AACAAGAATA ACCTGATCCC TGATGACAGA GCCAACTTCT ACCCGTTGCA GCAGACCAAC 
GTGTACACGA CCACCTACTA CCCAAGCCCA CTGAACAAAC ACAGCTTCCG GCCTGAGGCC 
TCACCTGGAC AACGGTGCTT CCCCAACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799504.1
CDS	102..3134
Translation	MRNYLFRLSLANVSLLQATEWASNEDTRRSCQSKGKTEEECQNYVRVLIVTGRKVFMCGTNAFSPVCSSRQVGNLSRTVEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSRPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPEVGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTTSRSLRGCYLEELHVLPPGRREPLRSLRIVHSARALFVGLSDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLEDSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRSRACDSPRPRCGGLDCLGPAVHIANCSRNGAWTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGVQSRRRTCENGNSCPGCGVEFKTCNPEACPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLPDPHGLQFGRRRMETRTCPADGSGACDTDALVEDLLRSGGASPLMVSGGWAAWGPWSSCSRDCELGFRFRKRTCTNPEPRNGGLPCVGDAAEYQDCNPHACPVRGAWSCWTAWSPCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCATQACPEGWSPWSEWSACTEDGVQSRDRHCEELVPGPSACAGNSSQSRPCPYSEIPVILPASSVDEATSCGGFSLIHLVATGISCFLGSGLLTLAVYLSCQHCQHQSQESTLVHPATPNHLHYKGGGTPKNEKYMPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKHSFRPEASPGQRCFPNS

Target ORF information:

RefSeq Version	XM_010801202.2
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 5B (SEMA5B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801202.2

ORF Insert Sequence:

ATGAGGAACT ACCTCTTCAG ACTCAGCCTT GCCAATGTCT CCCTCCTTCA GGCCACAGAG 
TGGGCCTCCA ACGAGGACAC GCGCCGCTCC TGCCAAAGCA AAGGGAAGAC TGAGGAGGAG 
TGTCAGAACT ACGTGCGAGT CCTGATTGTC ACTGGCCGGA AAGTGTTCAT GTGTGGGACC 
AATGCCTTTT CCCCAGTGTG CTCCAGCAGA CAGGTGGGGA ACCTCAGCCG GACGGTGGAG 
AAGATCAATG GTGTGGCCCG CTGCCCCTAT GACCCACGCC ACAACTCCAC TGCCGTCATC 
TCCTCTCAGG GGGAGCTCTA TGCTGCCACA GTCATCGACT TCTCAGGTCG GGACCCTGCT 
ATCTATCGCA GCCTGGGCAG CCGGCCACCA CTTCGCACTG CCCAGTATAA CTCCAAGTGG 
CTTAATGAGC CAAACTTTGT TGCAGCCTAT GATATCGGGC TGTTTGCATA CTTCTTCCTG 
CGGGAGAACG CAGTGGAGCA CGACTGTGGA CGCACCGTGT ACTCTCGGGT GGCCCGCGTG 
TGCAAGAACG ATGTGGGGGG CCGCTTCCTG CTGGAGGACA CTTGGACCAC GTTCATGAAG 
GCCCGGCTCA ACTGCTCCCG CCCGGGTGAG GTCCCCTTCT ACTACAACGA GCTGCAGAGC 
GCCTTCCATC TGCCCGAGCA GGACCTCATC TACGGGGTCT TCACCACCAA CGTAAACAGC 
ATCGCTGCGT CTGCTGTCTG CGCCTTCAAT CTCAGTGCCA TCTCCCAGGC TTTCAATGGC 
CCGTTTCGCT ACCAGGAGAA CCCCAGGGCT GCCTGGCTCC CCATCGCCAA CCCCATCCCC 
AATTTCCAGT GCGGCACCCT GCCGGAGGTG GGCCCCAATG AGAACCTGAC GGAGCGCAGC 
CTGCAGGACG CACAGCGCCT GTTCCTGATG AGCGAGGCTG TGCAGCCGGT GACACCGGAG 
CCTTGCGTCA CCCAAGACAG CGTGCGCTTC TCACATCTCG TGGTGGACCT TGTGCAGGCC 
AAGGATACGC TCTACCACGT GCTCTACATC GGCACCGAGT CGGGCACCAT CCTGAAGGCA 
CTGTCCACGA CGAGCCGCAG CCTCCGCGGT TGCTACCTGG AGGAGCTGCA CGTGCTGCCC 
CCCGGGCGCC GGGAGCCACT GCGCAGCCTG CGCATCGTGC ACAGCGCCCG CGCGCTCTTC 
GTGGGGTTGA GCGACGGTGT GCTGCGCGTC CCGCTGGAGA GGTGCGCCGC CTACCGCAGC 
CAGGGGGCCT GCCTGGGGGC ACGGGACCCG TACTGCGGCT GGGATGGAAA GCAGCAACGT 
TGCAGCACTC TCGAGGACAG CTCCAACATG AGCCTCTGGA CCCAGAACAT CACAGCATGT 
CCTGTGCGAA ATGTGACTCG GGATGGGGGC TTCGGCCCAT GGTCACCATG GCAACCATGT 
GAGCACTTGG ATGGGGACAA CTCAGGCTCT TGCTTGTGTC GGTCCCGAGC CTGTGACTCC 
CCTCGACCCC GCTGTGGGGG CCTCGACTGC CTGGGGCCAG CCGTTCACAT TGCCAACTGC 
TCCAGGAACG GGGCATGGAC ACCGTGGTCG TCTTGGGCCC TGTGCAGCAC CTCCTGTGGG 
ATCGGATTCC AGGTCCGCCA GCGAAGCTGC AGCAACCCCG CGCCACGCCA CGGGGGCCGC 
ATCTGCGTGG GCAAGAGCCG GGAGGAGCGG TTCTGTAACG AAAACACGCC TTGCCCGGTG 
CCCATCTTCT GGGCATCCTG GGGCTCCTGG AGCAAGTGCA GCAGCAACTG TGGGGGCGGC 
GTGCAGTCGC GCCGCCGGAC CTGTGAGAAC GGCAACTCCT GCCCGGGCTG CGGCGTGGAG 
TTCAAGACAT GCAATCCCGA GGCCTGCCCC GAAGTGCGGC GCAACACGCC GTGGACGCCG 
TGGCTGCCGG TGAACGTGAC CCAGGGCGGG GCGCGGCAGG AGCAGCGCTT CCGCTTCACT 
TGCCGCGCGC CGCTGCCCGA CCCCCACGGG CTGCAGTTTG GCCGAAGGAG GATGGAAACC 
CGAACCTGCC CGGCGGACGG CTCAGGAGCC TGCGACACTG ACGCCCTGGT GGAGGATCTC 
CTGCGCAGCG GGGGCGCCTC CCCGCTCATG GTGAGCGGGG GCTGGGCCGC CTGGGGCCCA 
TGGTCCTCCT GCTCCCGGGA CTGTGAGCTG GGTTTCCGCT TCCGGAAGAG AACATGCACG 
AACCCCGAGC CCCGCAACGG GGGCCTGCCC TGCGTGGGTG ATGCCGCCGA GTATCAGGAC 
TGCAACCCGC ACGCTTGTCC AGTGCGGGGT GCCTGGTCTT GCTGGACCGC TTGGTCCCCG 
TGCTCTGCCT CCTGCGGTGG CGGCCACTAC CAACGCACCC GTTCCTGTAC CAGCCCCGCG 
CCTTCCCCCG GCGAGGACAT TTGTCTCGGG CTGCACACGG AGGAGGCGCT GTGTGCCACA 
CAGGCCTGCC CAGAAGGCTG GTCGCCGTGG TCTGAGTGGA GTGCATGCAC TGAGGATGGA 
GTGCAAAGCC GCGACAGGCA CTGTGAGGAG CTGGTCCCTG GGCCCAGCGC TTGTGCTGGA 
AACAGCAGCC AGAGCCGGCC CTGCCCTTAC AGCGAGATTC CCGTGATCCT GCCTGCCTCC 
AGTGTGGATG AGGCCACCAG CTGTGGAGGG TTCAGTCTCA TCCACCTGGT GGCCACGGGT 
ATCTCCTGCT TCCTGGGCTC TGGGCTGCTG ACCTTGGCGG TGTACCTGTC CTGCCAGCAT 
TGCCAGCACC AGTCCCAGGA GTCCACGCTC GTCCATCCCG CCACCCCCAA CCACCTGCAC 
TACAAGGGTG GGGGCACCCC CAAGAATGAG AAGTACATGC CCATGGAATT CAAGACCCTG 
AACAAGAATA ACCTGATCCC TGATGACAGA GCCAACTTCT ACCCGTTGCA GCAGACCAAC 
GTGTACACGA CCACCTACTA CCCAAGCCCA CTGAACAAAC ACAGCTTCCG GCCTGAGGCC 
TCACCTGGAC AACGGTGCTT CCCCAACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5B ( XM_010824635.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822937.1 Bos taurus semaphorin 5B (SEMA5B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa57547
Clone ID Related Accession (Same CDS sequence)	XM_010801202.2 , XM_010824635.2
Accession Version	XM_010824635.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	semaphorin-5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644520.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824635.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAACT ACCTCTTCAG ACTCAGCCTT GCCAATGTCT CCCTCCTTCA GGCCACAGAG 
TGGGCCTCCA ACGAGGACAC GCGCCGCTCC TGCCAAAGCA AAGGGAAGAC TGAGGAGGAG 
TGTCAGAACT ACGTGCGAGT CCTGATTGTC ACTGGCCGGA AAGTGTTCAT GTGTGGGACC 
AATGCCTTTT CCCCAGTGTG CTCCAGCAGA CAGGTGGGGA ACCTCAGCCG GACGGTGGAG 
AAGATCAATG GTGTGGCCCG CTGCCCCTAT GACCCACGCC ACAACTCCAC TGCCGTCATC 
TCCTCTCAGG GGGAGCTCTA TGCTGCCACA GTCATCGACT TCTCAGGTCG GGACCCTGCT 
ATCTATCGCA GCCTGGGCAG CCGGCCACCA CTTCGCACTG CCCAGTATAA CTCCAAGTGG 
CTTAATGAGC CAAACTTTGT TGCAGCCTAT GATATCGGGC TGTTTGCATA CTTCTTCCTG 
CGGGAGAACG CAGTGGAGCA CGACTGTGGA CGCACCGTGT ACTCTCGGGT GGCCCGCGTG 
TGCAAGAACG ATGTGGGGGG CCGCTTCCTG CTGGAGGACA CTTGGACCAC GTTCATGAAG 
GCCCGGCTCA ACTGCTCCCG CCCGGGTGAG GTCCCCTTCT ACTACAACGA GCTGCAGAGC 
GCCTTCCATC TGCCCGAGCA GGACCTCATC TACGGGGTCT TCACCACCAA CGTAAACAGC 
ATCGCTGCGT CTGCTGTCTG CGCCTTCAAT CTCAGTGCCA TCTCCCAGGC TTTCAATGGC 
CCGTTTCGCT ACCAGGAGAA CCCCAGGGCT GCCTGGCTCC CCATCGCCAA CCCCATCCCC 
AATTTCCAGT GCGGCACCCT GCCGGAGGTG GGCCCCAATG AGAACCTGAC GGAGCGCAGC 
CTGCAGGACG CACAGCGCCT GTTCCTGATG AGCGAGGCTG TGCAGCCGGT GACACCGGAG 
CCTTGCGTCA CCCAAGACAG CGTGCGCTTC TCACATCTCG TGGTGGACCT TGTGCAGGCC 
AAGGATACGC TCTACCACGT GCTCTACATC GGCACCGAGT CGGGCACCAT CCTGAAGGCA 
CTGTCCACGA CGAGCCGCAG CCTCCGCGGT TGCTACCTGG AGGAGCTGCA CGTGCTGCCC 
CCCGGGCGCC GGGAGCCACT GCGCAGCCTG CGCATCGTGC ACAGCGCCCG CGCGCTCTTC 
GTGGGGTTGA GCGACGGTGT GCTGCGCGTC CCGCTGGAGA GGTGCGCCGC CTACCGCAGC 
CAGGGGGCCT GCCTGGGGGC ACGGGACCCG TACTGCGGCT GGGATGGAAA GCAGCAACGT 
TGCAGCACTC TCGAGGACAG CTCCAACATG AGCCTCTGGA CCCAGAACAT CACAGCATGT 
CCTGTGCGAA ATGTGACTCG GGATGGGGGC TTCGGCCCAT GGTCACCATG GCAACCATGT 
GAGCACTTGG ATGGGGACAA CTCAGGCTCT TGCTTGTGTC GGTCCCGAGC CTGTGACTCC 
CCTCGACCCC GCTGTGGGGG CCTCGACTGC CTGGGGCCAG CCGTTCACAT TGCCAACTGC 
TCCAGGAACG GGGCATGGAC ACCGTGGTCG TCTTGGGCCC TGTGCAGCAC CTCCTGTGGG 
ATCGGATTCC AGGTCCGCCA GCGAAGCTGC AGCAACCCCG CGCCACGCCA CGGGGGCCGC 
ATCTGCGTGG GCAAGAGCCG GGAGGAGCGG TTCTGTAACG AAAACACGCC TTGCCCGGTG 
CCCATCTTCT GGGCATCCTG GGGCTCCTGG AGCAAGTGCA GCAGCAACTG TGGGGGCGGC 
GTGCAGTCGC GCCGCCGGAC CTGTGAGAAC GGCAACTCCT GCCCGGGCTG CGGCGTGGAG 
TTCAAGACAT GCAATCCCGA GGCCTGCCCC GAAGTGCGGC GCAACACGCC GTGGACGCCG 
TGGCTGCCGG TGAACGTGAC CCAGGGCGGG GCGCGGCAGG AGCAGCGCTT CCGCTTCACT 
TGCCGCGCGC CGCTGCCCGA CCCCCACGGG CTGCAGTTTG GCCGAAGGAG GATGGAAACC 
CGAACCTGCC CGGCGGACGG CTCAGGAGCC TGCGACACTG ACGCCCTGGT GGAGGATCTC 
CTGCGCAGCG GGGGCGCCTC CCCGCTCATG GTGAGCGGGG GCTGGGCCGC CTGGGGCCCA 
TGGTCCTCCT GCTCCCGGGA CTGTGAGCTG GGTTTCCGCT TCCGGAAGAG AACATGCACG 
AACCCCGAGC CCCGCAACGG GGGCCTGCCC TGCGTGGGTG ATGCCGCCGA GTATCAGGAC 
TGCAACCCGC ACGCTTGTCC AGTGCGGGGT GCCTGGTCTT GCTGGACCGC TTGGTCCCCG 
TGCTCTGCCT CCTGCGGTGG CGGCCACTAC CAACGCACCC GTTCCTGTAC CAGCCCCGCG 
CCTTCCCCCG GCGAGGACAT TTGTCTCGGG CTGCACACGG AGGAGGCGCT GTGTGCCACA 
CAGGCCTGCC CAGAAGGCTG GTCGCCGTGG TCTGAGTGGA GTGCATGCAC TGAGGATGGA 
GTGCAAAGCC GCGACAGGCA CTGTGAGGAG CTGGTCCCTG GGCCCAGCGC TTGTGCTGGA 
AACAGCAGCC AGAGCCGGCC CTGCCCTTAC AGCGAGATTC CCGTGATCCT GCCTGCCTCC 
AGTGTGGATG AGGCCACCAG CTGTGGAGGG TTCAGTCTCA TCCACCTGGT GGCCACGGGT 
ATCTCCTGCT TCCTGGGCTC TGGGCTGCTG ACCTTGGCGG TGTACCTGTC CTGCCAGCAT 
TGCCAGCACC AGTCCCAGGA GTCCACGCTC GTCCATCCCG CCACCCCCAA CCACCTGCAC 
TACAAGGGTG GGGGCACCCC CAAGAATGAG AAGTACATGC CCATGGAATT CAAGACCCTG 
AACAAGAATA ACCTGATCCC TGATGACAGA GCCAACTTCT ACCCGTTGCA GCAGACCAAC 
GTGTACACGA CCACCTACTA CCCAAGCCCA CTGAACAAAC ACAGCTTCCG GCCTGAGGCC 
TCACCTGGAC AACGGTGCTT CCCCAACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822937.1
CDS	102..3134
Translation	MRNYLFRLSLANVSLLQATEWASNEDTRRSCQSKGKTEEECQNYVRVLIVTGRKVFMCGTNAFSPVCSSRQVGNLSRTVEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSRPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPEVGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTTSRSLRGCYLEELHVLPPGRREPLRSLRIVHSARALFVGLSDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLEDSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRSRACDSPRPRCGGLDCLGPAVHIANCSRNGAWTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGVQSRRRTCENGNSCPGCGVEFKTCNPEACPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLPDPHGLQFGRRRMETRTCPADGSGACDTDALVEDLLRSGGASPLMVSGGWAAWGPWSSCSRDCELGFRFRKRTCTNPEPRNGGLPCVGDAAEYQDCNPHACPVRGAWSCWTAWSPCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCATQACPEGWSPWSEWSACTEDGVQSRDRHCEELVPGPSACAGNSSQSRPCPYSEIPVILPASSVDEATSCGGFSLIHLVATGISCFLGSGLLTLAVYLSCQHCQHQSQESTLVHPATPNHLHYKGGGTPKNEKYMPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKHSFRPEASPGQRCFPNS

Target ORF information:

RefSeq Version	XM_010824635.2
Organism	Bos taurus(cattle)
Definition	Bos taurus semaphorin 5B (SEMA5B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824635.2

ORF Insert Sequence:

ATGAGGAACT ACCTCTTCAG ACTCAGCCTT GCCAATGTCT CCCTCCTTCA GGCCACAGAG 
TGGGCCTCCA ACGAGGACAC GCGCCGCTCC TGCCAAAGCA AAGGGAAGAC TGAGGAGGAG 
TGTCAGAACT ACGTGCGAGT CCTGATTGTC ACTGGCCGGA AAGTGTTCAT GTGTGGGACC 
AATGCCTTTT CCCCAGTGTG CTCCAGCAGA CAGGTGGGGA ACCTCAGCCG GACGGTGGAG 
AAGATCAATG GTGTGGCCCG CTGCCCCTAT GACCCACGCC ACAACTCCAC TGCCGTCATC 
TCCTCTCAGG GGGAGCTCTA TGCTGCCACA GTCATCGACT TCTCAGGTCG GGACCCTGCT 
ATCTATCGCA GCCTGGGCAG CCGGCCACCA CTTCGCACTG CCCAGTATAA CTCCAAGTGG 
CTTAATGAGC CAAACTTTGT TGCAGCCTAT GATATCGGGC TGTTTGCATA CTTCTTCCTG 
CGGGAGAACG CAGTGGAGCA CGACTGTGGA CGCACCGTGT ACTCTCGGGT GGCCCGCGTG 
TGCAAGAACG ATGTGGGGGG CCGCTTCCTG CTGGAGGACA CTTGGACCAC GTTCATGAAG 
GCCCGGCTCA ACTGCTCCCG CCCGGGTGAG GTCCCCTTCT ACTACAACGA GCTGCAGAGC 
GCCTTCCATC TGCCCGAGCA GGACCTCATC TACGGGGTCT TCACCACCAA CGTAAACAGC 
ATCGCTGCGT CTGCTGTCTG CGCCTTCAAT CTCAGTGCCA TCTCCCAGGC TTTCAATGGC 
CCGTTTCGCT ACCAGGAGAA CCCCAGGGCT GCCTGGCTCC CCATCGCCAA CCCCATCCCC 
AATTTCCAGT GCGGCACCCT GCCGGAGGTG GGCCCCAATG AGAACCTGAC GGAGCGCAGC 
CTGCAGGACG CACAGCGCCT GTTCCTGATG AGCGAGGCTG TGCAGCCGGT GACACCGGAG 
CCTTGCGTCA CCCAAGACAG CGTGCGCTTC TCACATCTCG TGGTGGACCT TGTGCAGGCC 
AAGGATACGC TCTACCACGT GCTCTACATC GGCACCGAGT CGGGCACCAT CCTGAAGGCA 
CTGTCCACGA CGAGCCGCAG CCTCCGCGGT TGCTACCTGG AGGAGCTGCA CGTGCTGCCC 
CCCGGGCGCC GGGAGCCACT GCGCAGCCTG CGCATCGTGC ACAGCGCCCG CGCGCTCTTC 
GTGGGGTTGA GCGACGGTGT GCTGCGCGTC CCGCTGGAGA GGTGCGCCGC CTACCGCAGC 
CAGGGGGCCT GCCTGGGGGC ACGGGACCCG TACTGCGGCT GGGATGGAAA GCAGCAACGT 
TGCAGCACTC TCGAGGACAG CTCCAACATG AGCCTCTGGA CCCAGAACAT CACAGCATGT 
CCTGTGCGAA ATGTGACTCG GGATGGGGGC TTCGGCCCAT GGTCACCATG GCAACCATGT 
GAGCACTTGG ATGGGGACAA CTCAGGCTCT TGCTTGTGTC GGTCCCGAGC CTGTGACTCC 
CCTCGACCCC GCTGTGGGGG CCTCGACTGC CTGGGGCCAG CCGTTCACAT TGCCAACTGC 
TCCAGGAACG GGGCATGGAC ACCGTGGTCG TCTTGGGCCC TGTGCAGCAC CTCCTGTGGG 
ATCGGATTCC AGGTCCGCCA GCGAAGCTGC AGCAACCCCG CGCCACGCCA CGGGGGCCGC 
ATCTGCGTGG GCAAGAGCCG GGAGGAGCGG TTCTGTAACG AAAACACGCC TTGCCCGGTG 
CCCATCTTCT GGGCATCCTG GGGCTCCTGG AGCAAGTGCA GCAGCAACTG TGGGGGCGGC 
GTGCAGTCGC GCCGCCGGAC CTGTGAGAAC GGCAACTCCT GCCCGGGCTG CGGCGTGGAG 
TTCAAGACAT GCAATCCCGA GGCCTGCCCC GAAGTGCGGC GCAACACGCC GTGGACGCCG 
TGGCTGCCGG TGAACGTGAC CCAGGGCGGG GCGCGGCAGG AGCAGCGCTT CCGCTTCACT 
TGCCGCGCGC CGCTGCCCGA CCCCCACGGG CTGCAGTTTG GCCGAAGGAG GATGGAAACC 
CGAACCTGCC CGGCGGACGG CTCAGGAGCC TGCGACACTG ACGCCCTGGT GGAGGATCTC 
CTGCGCAGCG GGGGCGCCTC CCCGCTCATG GTGAGCGGGG GCTGGGCCGC CTGGGGCCCA 
TGGTCCTCCT GCTCCCGGGA CTGTGAGCTG GGTTTCCGCT TCCGGAAGAG AACATGCACG 
AACCCCGAGC CCCGCAACGG GGGCCTGCCC TGCGTGGGTG ATGCCGCCGA GTATCAGGAC 
TGCAACCCGC ACGCTTGTCC AGTGCGGGGT GCCTGGTCTT GCTGGACCGC TTGGTCCCCG 
TGCTCTGCCT CCTGCGGTGG CGGCCACTAC CAACGCACCC GTTCCTGTAC CAGCCCCGCG 
CCTTCCCCCG GCGAGGACAT TTGTCTCGGG CTGCACACGG AGGAGGCGCT GTGTGCCACA 
CAGGCCTGCC CAGAAGGCTG GTCGCCGTGG TCTGAGTGGA GTGCATGCAC TGAGGATGGA 
GTGCAAAGCC GCGACAGGCA CTGTGAGGAG CTGGTCCCTG GGCCCAGCGC TTGTGCTGGA 
AACAGCAGCC AGAGCCGGCC CTGCCCTTAC AGCGAGATTC CCGTGATCCT GCCTGCCTCC 
AGTGTGGATG AGGCCACCAG CTGTGGAGGG TTCAGTCTCA TCCACCTGGT GGCCACGGGT 
ATCTCCTGCT TCCTGGGCTC TGGGCTGCTG ACCTTGGCGG TGTACCTGTC CTGCCAGCAT 
TGCCAGCACC AGTCCCAGGA GTCCACGCTC GTCCATCCCG CCACCCCCAA CCACCTGCAC 
TACAAGGGTG GGGGCACCCC CAAGAATGAG AAGTACATGC CCATGGAATT CAAGACCCTG 
AACAAGAATA ACCTGATCCC TGATGACAGA GCCAACTTCT ACCCGTTGCA GCAGACCAAC 
GTGTACACGA CCACCTACTA CCCAAGCCCA CTGAACAAAC ACAGCTTCCG GCCTGAGGCC 
TCACCTGGAC AACGGTGCTT CCCCAACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SEMA5B cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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