DENND5A cDNA ORF clone, Bos taurus(cattle)

The following DENND5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DENND5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa223233 XM_002693106.5
Latest version!
Bos taurus DENN domain containing 5A (DENND5A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223234 XM_024975808.1
Latest version!
Bos taurus DENN domain containing 5A (DENND5A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223235 XM_005216096.4
Latest version!
Bos taurus DENN domain containing 5A (DENND5A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa26409 XM_002693106.4
Latest version!
Bos taurus DENN/MADD domain containing 5A (DENND5A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa26409 XM_001252217.6
Latest version!
Bos taurus DENN/MADD domain containing 5A (DENND5A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa26411 XM_005194609.3
Latest version!
Bos taurus DENN/MADD domain containing 5A (DENND5A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa26411 XM_005216096.3
Latest version!
Bos taurus DENN/MADD domain containing 5A (DENND5A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa57545 XM_010822806.2
Latest version!
Bos taurus DENN/MADD domain containing 5A (DENND5A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa57545 XM_010812548.2
Latest version!
Bos taurus DENN/MADD domain containing 5A (DENND5A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa223233
    Clone ID Related Accession (Same CDS sequence) XM_002693106.5
    Accession Version XM_002693106.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DENN domain-containing protein 5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002693106.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCGGCG GCGGCGGCGG CGGCGGCGGA GGGGGCTCGG CGCCCAGTCG CTTCGCCGAC 
    TACTTTGTCA TCTGCGGGCT GGACACCGAG ACCGGGCTGG AGCCGGACGA GCTGTCGGCA
    TTATGCCAGT ACATACAGGC TTCTAAAGCC AGGGATGGTG CCAGCTCTTT CATTTCAAGT
    ACAACTGAAG GAGAAAATTT TGAGCAGACA CCGTTGAGAC GAACATTCAA GTCTAAGGTC
    CTTGCACGAT ATCCTGAGAA TGTGGACTGG AATCCCTTTG ACCAAGATGC AGTGGGAATG
    CTATGCATGC CCAAAGGGCT GGCATTCAAG ACTCAGGCTG ATCCCAGGGA GCCTCAGTTC
    CATGCCTTTA TTATCACTAG GGAGGATGGC TCTCGGACCT TTGGGTTTGC TCTCACGTTT
    TATGAGGAGG TGACTAGCAA GCAGATCTGC AGTGCAATGC AGACCCTCTA CCACATGCAT
    AATGCAGAGT ATGATGTCCT CCATGCTCCA CTTGCTGATA GCCGAGACCT CAGTGGCATG
    GAGGATGGTG ATGGCACTCC TGGGACCAAG CTGCAGCGCT TCAACTCCTA CGACATTAGC
    AGAGATACAC TCTACGTCTC TAAGTGCATC TGCCTCATCA CGCCCATGTC CTTCATGAAA
    GCATGTCGGA GTGTGCTGGA ACAGCTTCAC CAGGCAGTCA CTTCGCCTCA GCCCCCTCCA
    CTGCCCCTTG AGAGCTACAT ATACAATGTA CTATATGAGG TGCCACTCCC ACCTCCTGGC
    CGGTCCTTGA AGTTTTCTGG TGTCTATGGG CCAATCGTCT GCCAGAGACC AAGTACCAAT
    GAGCTTCCCC TGTTCGACTT TCCTGTCAAA GAGGTCTTTG AACTGCTTGG GGTGGAGAAT
    GTGTTTCAAC TTTTTACTTG TGCCCTTCTG GAGTTTCAAA TCCTGCTCTA CTCACAGCAT
    TACCAGCGAC TGATGACTGT GGCGGAGACA ATTACAGCTC TCATGTTTCC TTTCCAGTGG
    CAGCATGTCT ATGTTCCTAT TCTCCCGGCT TCTCTCCTAC ATTTCTTAGA TGCTCCTGTG
    CCATACTTGA TGGGCTTGCA TTCCAATGGC TTGGATGACC GGTCAAAACT GGAGCTGCCT
    CAAGAGGCTA ACCTCTGCTT TGTGGACATT GACAACCACT TCATTGAGTT ACCAGAGGAC
    TTGCCTCAGT TTCCCAACAA ATTGGAGTTT GTCCAGGAAG TCTCTGAGAT TCTCATGGCA
    TTTGGCATTC CCCCTGAAGG AAGCCTTCAC TGTAGCGAGA GTGCTTCCAA GCTGAAGAGG
    CTCCGGGCCT CTGAACTCGT CTCTGACAAG AGGAATGGAA ACATCGCAGG CTCCCCTTTA
    CATTCCTATG AGTTCCTCAA GGAGAATGAA ACTATCGCCC GCCTGCAGGC CTTGGTCAAG
    AGGACTGGGG TGAGCCTGGA GAAGTTGGAA GTGCGTGAAG ACCCCAGTAG CAATAAGGAT
    CTCAAAGTTC AGTGTGATGA AGAAGAACTC AGGATTTACC AGCTAAACAT TCAGATCCGG
    GAAGTTTTTG CAAATCGTTT CACTCAGATG TTTGCAGATT ATGAGGTGTT TGTCATTCAG
    CCCAGCCAGG ATAAGGAGTC CTGGTTTACC AACAGAGAGC AGATGCAAAA TTTTGATAAA
    GCATCTTTTC TGTCAGATCA GCCTGAACCC TACCTGCCTT TCCTCTCAAG ATTCTTGGAG
    ACTCAGATGT TTGCTTCTTT CATTGACAAC AAAATCATGT GTCATGACGA TGATGATAAA
    GATCCTGTGC TCCGGGTGTT TGACTCCCGA GTAGACAAGA TCAGGCTGTT GAATGTTCGG
    ACACCTACTC TGCGCACATC CATGTACCAG AAGTGTACCA CAGTGGATGA AGCAAAGAAA
    GCAATTGAGC TGCGATTGGC AAAAATTGAT CATACTGCGG TTCATCCCCA TTTACTTGAC
    ATGAAGATTG GACAAGGGAA GTATGAACCA GGTTTCTTCC CTAAGCTACA GTCTGACGTG
    CTTTCCACTG GGCCAGCCAG CAACAAGTGG ACAAAAAGAA ATGCTCCTGC CCAGTGGAGG
    CGTAAAGACC GGCAGAAGCA GCACACAGAA CACCTGCGCT TAGACAATGA CCAGAGAGAG
    AAGTACATCC AGGAAGCCAG GAATATGGGC AGCACCATCC GCCAGCCCAA ACTGTCCAAC
    CTCTCCCCGT CAGTGATTGC CCAGACCAAC TGGAAGTTTG TGGAAGGTCT GCTGAAGGAA
    TGCCGCAACA AGACCAAGAG GATGCTGGTG GAGAAGATGG GGCGAGAGGC TGTGGAACTA
    GGCCATGGGG AAGTGAACAT CACGGGGGTG GAAGAGAACA CCTTGATTGC CAGTCTTTGT
    GACCTACTGG AAAGGATTTG GAGTCATGGG CTACAGGTGA AACAGGGGAA ATCAGCCTTG
    TGGTCCCACC TGTTACATTA TCAGGAAAAC CGGCAGAGAA AACTCACATC AGGAAGCCTC
    AGTACCTCAG GAATATTTCT TGATTCAGAG CGGAGGAAGT CTGATGCCAG CTCCGTCATG
    TCTCCCCTGA AAATCTCCCT CATTCAGGAT ATGAGGCACA TCCAGAACAT TGGGGAAATC
    AAGACTGATG TGGGAAAAGC CAGAGCGTGG GTGCGTCTGT CCATGGAAAA AAAGTTACTT
    TCTAGACACC TGAAGCAGCT TCTCTCAGAC CATGAGCTCA CCAAAAAACT CTACAAGCGC
    TACGCCTTCC TGCGCTGTGA CGACGAGAAG GAGCAGTTCC TCTATCACCT CCTCTCTTTC
    AATGCCGTCG ATTACTTCTG CTTCACCAAT GTCTTCACGA CTATCCTGAT TCCTTACCAC
    ATCCTGATTG TACCAAGCAA GAAGCTGGGG GGCTCTATGT TCACCGCCAA CCCATGGATC
    TGCATCTCAG GAGAACTGGG TGAGACACAG ATCCTGCAGA TTCCCAGGAA CCTGTTAGAG
    ATGACCTTCG AGTGCCAAAA CTTGGGGAAA CTCACTACTG TGCAGATAGG CCATGATAAC
    TCTGGACTCT ACGCCAAATG GTTGGTGGAA TATGTGATGG TTAGGAATGA GATCACCGGA
    CATACCTACA AGTTTCCATG TGGCCGCTGG TTGGGGAAGG GCATGGATGA CGGGAGCCTG
    GAGCGGGTTC TGGTCGGGGA GCTGCTCACA TCCCAGCCTG AGGTGGATGA GAGGCCGTGC
    CGGACTCCGC CCCTGCAGCA GTCTCCCAGC GTCATCCGGA GGCTTGTTAC CATCTCACCC
    AACAACAGAC CCAAGCTGAA CACTGGGCAG ATCCAAGAGT CCATCGGTGA GGCAGTCAAT
    GGCATCGTGA AGCACTTCCA TAAGCCTGAA AAAGAGCGAG GTAGTCTGAC GCTGTTGCTT
    TGTGGAGAAT GTGGCCTTGT CTCGGCCCTG GAACAGGCTT TCCAGCATGG ATTTAAGTCA
    CCACGGCTCT TCAAAAATGT CTTCATTTGG GATTTTTTGG AAAAAGCACA AACCTATTAT
    GAGACTTTGG AGCAGAACGA ACTTGTCCCT GAGGAAAACT GGCATATGAG GGCCCGGAAC
    TTCTGCCGAT TTGTCACGGC AATCAACAAT AATCCCCGGA ACATTGGCAA GGATGGCAAG
    TTTCAGATGC TGGTGTGCTT GGGAGCCAGA GACCACCTCC TGCACCACTG GATCGCCCTG
    CTGGCTGACT GCCCCATCAC CGCACACATG TATGAGGATG TGGCACTGAT CAAAGACCAC
    ACACTTGTCA ATTCCTTGAT CCGCGTGCTG CAGACACTGC AGGAGTTCAA CATCACGCTG
    GAGACATCTC TGGTCAAGGG CATCGATATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002693152.2
    CDS35..3907
    Translation

    Target ORF information:

    RefSeq Version XM_002693106.5
    Organism Bos taurus(cattle)
    Definition Bos taurus DENN domain containing 5A (DENND5A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002693106.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCGGCG GCGGCGGCGG CGGCGGCGGA GGGGGCTCGG CGCCCAGTCG CTTCGCCGAC 
    TACTTTGTCA TCTGCGGGCT GGACACCGAG ACCGGGCTGG AGCCGGACGA GCTGTCGGCA
    TTATGCCAGT ACATACAGGC TTCTAAAGCC AGGGATGGTG CCAGCTCTTT CATTTCAAGT
    ACAACTGAAG GAGAAAATTT TGAGCAGACA CCGTTGAGAC GAACATTCAA GTCTAAGGTC
    CTTGCACGAT ATCCTGAGAA TGTGGACTGG AATCCCTTTG ACCAAGATGC AGTGGGAATG
    CTATGCATGC CCAAAGGGCT GGCATTCAAG ACTCAGGCTG ATCCCAGGGA GCCTCAGTTC
    CATGCCTTTA TTATCACTAG GGAGGATGGC TCTCGGACCT TTGGGTTTGC TCTCACGTTT
    TATGAGGAGG TGACTAGCAA GCAGATCTGC AGTGCAATGC AGACCCTCTA CCACATGCAT
    AATGCAGAGT ATGATGTCCT CCATGCTCCA CTTGCTGATA GCCGAGACCT CAGTGGCATG
    GAGGATGGTG ATGGCACTCC TGGGACCAAG CTGCAGCGCT TCAACTCCTA CGACATTAGC
    AGAGATACAC TCTACGTCTC TAAGTGCATC TGCCTCATCA CGCCCATGTC CTTCATGAAA
    GCATGTCGGA GTGTGCTGGA ACAGCTTCAC CAGGCAGTCA CTTCGCCTCA GCCCCCTCCA
    CTGCCCCTTG AGAGCTACAT ATACAATGTA CTATATGAGG TGCCACTCCC ACCTCCTGGC
    CGGTCCTTGA AGTTTTCTGG TGTCTATGGG CCAATCGTCT GCCAGAGACC AAGTACCAAT
    GAGCTTCCCC TGTTCGACTT TCCTGTCAAA GAGGTCTTTG AACTGCTTGG GGTGGAGAAT
    GTGTTTCAAC TTTTTACTTG TGCCCTTCTG GAGTTTCAAA TCCTGCTCTA CTCACAGCAT
    TACCAGCGAC TGATGACTGT GGCGGAGACA ATTACAGCTC TCATGTTTCC TTTCCAGTGG
    CAGCATGTCT ATGTTCCTAT TCTCCCGGCT TCTCTCCTAC ATTTCTTAGA TGCTCCTGTG
    CCATACTTGA TGGGCTTGCA TTCCAATGGC TTGGATGACC GGTCAAAACT GGAGCTGCCT
    CAAGAGGCTA ACCTCTGCTT TGTGGACATT GACAACCACT TCATTGAGTT ACCAGAGGAC
    TTGCCTCAGT TTCCCAACAA ATTGGAGTTT GTCCAGGAAG TCTCTGAGAT TCTCATGGCA
    TTTGGCATTC CCCCTGAAGG AAGCCTTCAC TGTAGCGAGA GTGCTTCCAA GCTGAAGAGG
    CTCCGGGCCT CTGAACTCGT CTCTGACAAG AGGAATGGAA ACATCGCAGG CTCCCCTTTA
    CATTCCTATG AGTTCCTCAA GGAGAATGAA ACTATCGCCC GCCTGCAGGC CTTGGTCAAG
    AGGACTGGGG TGAGCCTGGA GAAGTTGGAA GTGCGTGAAG ACCCCAGTAG CAATAAGGAT
    CTCAAAGTTC AGTGTGATGA AGAAGAACTC AGGATTTACC AGCTAAACAT TCAGATCCGG
    GAAGTTTTTG CAAATCGTTT CACTCAGATG TTTGCAGATT ATGAGGTGTT TGTCATTCAG
    CCCAGCCAGG ATAAGGAGTC CTGGTTTACC AACAGAGAGC AGATGCAAAA TTTTGATAAA
    GCATCTTTTC TGTCAGATCA GCCTGAACCC TACCTGCCTT TCCTCTCAAG ATTCTTGGAG
    ACTCAGATGT TTGCTTCTTT CATTGACAAC AAAATCATGT GTCATGACGA TGATGATAAA
    GATCCTGTGC TCCGGGTGTT TGACTCCCGA GTAGACAAGA TCAGGCTGTT GAATGTTCGG
    ACACCTACTC TGCGCACATC CATGTACCAG AAGTGTACCA CAGTGGATGA AGCAAAGAAA
    GCAATTGAGC TGCGATTGGC AAAAATTGAT CATACTGCGG TTCATCCCCA TTTACTTGAC
    ATGAAGATTG GACAAGGGAA GTATGAACCA GGTTTCTTCC CTAAGCTACA GTCTGACGTG
    CTTTCCACTG GGCCAGCCAG CAACAAGTGG ACAAAAAGAA ATGCTCCTGC CCAGTGGAGG
    CGTAAAGACC GGCAGAAGCA GCACACAGAA CACCTGCGCT TAGACAATGA CCAGAGAGAG
    AAGTACATCC AGGAAGCCAG GAATATGGGC AGCACCATCC GCCAGCCCAA ACTGTCCAAC
    CTCTCCCCGT CAGTGATTGC CCAGACCAAC TGGAAGTTTG TGGAAGGTCT GCTGAAGGAA
    TGCCGCAACA AGACCAAGAG GATGCTGGTG GAGAAGATGG GGCGAGAGGC TGTGGAACTA
    GGCCATGGGG AAGTGAACAT CACGGGGGTG GAAGAGAACA CCTTGATTGC CAGTCTTTGT
    GACCTACTGG AAAGGATTTG GAGTCATGGG CTACAGGTGA AACAGGGGAA ATCAGCCTTG
    TGGTCCCACC TGTTACATTA TCAGGAAAAC CGGCAGAGAA AACTCACATC AGGAAGCCTC
    AGTACCTCAG GAATATTTCT TGATTCAGAG CGGAGGAAGT CTGATGCCAG CTCCGTCATG
    TCTCCCCTGA AAATCTCCCT CATTCAGGAT ATGAGGCACA TCCAGAACAT TGGGGAAATC
    AAGACTGATG TGGGAAAAGC CAGAGCGTGG GTGCGTCTGT CCATGGAAAA AAAGTTACTT
    TCTAGACACC TGAAGCAGCT TCTCTCAGAC CATGAGCTCA CCAAAAAACT CTACAAGCGC
    TACGCCTTCC TGCGCTGTGA CGACGAGAAG GAGCAGTTCC TCTATCACCT CCTCTCTTTC
    AATGCCGTCG ATTACTTCTG CTTCACCAAT GTCTTCACGA CTATCCTGAT TCCTTACCAC
    ATCCTGATTG TACCAAGCAA GAAGCTGGGG GGCTCTATGT TCACCGCCAA CCCATGGATC
    TGCATCTCAG GAGAACTGGG TGAGACACAG ATCCTGCAGA TTCCCAGGAA CCTGTTAGAG
    ATGACCTTCG AGTGCCAAAA CTTGGGGAAA CTCACTACTG TGCAGATAGG CCATGATAAC
    TCTGGACTCT ACGCCAAATG GTTGGTGGAA TATGTGATGG TTAGGAATGA GATCACCGGA
    CATACCTACA AGTTTCCATG TGGCCGCTGG TTGGGGAAGG GCATGGATGA CGGGAGCCTG
    GAGCGGGTTC TGGTCGGGGA GCTGCTCACA TCCCAGCCTG AGGTGGATGA GAGGCCGTGC
    CGGACTCCGC CCCTGCAGCA GTCTCCCAGC GTCATCCGGA GGCTTGTTAC CATCTCACCC
    AACAACAGAC CCAAGCTGAA CACTGGGCAG ATCCAAGAGT CCATCGGTGA GGCAGTCAAT
    GGCATCGTGA AGCACTTCCA TAAGCCTGAA AAAGAGCGAG GTAGTCTGAC GCTGTTGCTT
    TGTGGAGAAT GTGGCCTTGT CTCGGCCCTG GAACAGGCTT TCCAGCATGG ATTTAAGTCA
    CCACGGCTCT TCAAAAATGT CTTCATTTGG GATTTTTTGG AAAAAGCACA AACCTATTAT
    GAGACTTTGG AGCAGAACGA ACTTGTCCCT GAGGAAAACT GGCATATGAG GGCCCGGAAC
    TTCTGCCGAT TTGTCACGGC AATCAACAAT AATCCCCGGA ACATTGGCAA GGATGGCAAG
    TTTCAGATGC TGGTGTGCTT GGGAGCCAGA GACCACCTCC TGCACCACTG GATCGCCCTG
    CTGGCTGACT GCCCCATCAC CGCACACATG TATGAGGATG TGGCACTGAT CAAAGACCAC
    ACACTTGTCA ATTCCTTGAT CCGCGTGCTG CAGACACTGC AGGAGTTCAA CATCACGCTG
    GAGACATCTC TGGTCAAGGG CATCGATATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223234
    Clone ID Related Accession (Same CDS sequence) XM_024975808.1
    Accession Version XM_024975808.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DENN domain-containing protein 5A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTATGCA TGCCCAAAGG GCTGGCATTC AAGACTCAGG CTGATCCCAG GGAGCCTCAG 
    TTCCATGCCT TTATTATCAC TAGGGAGGAT GGCTCTCGGA CCTTTGGGTT TGCTCTCACG
    TTTTATGAGG AGGTGACTAG CAAGCAGATC TGCAGTGCAA TGCAGACCCT CTACCACATG
    CATAATGCAG AGTATGATGT CCTCCATGCT CCACTTGCTG ATAGCCGAGA CCTCAGTGGC
    ATGGAGGATG GTGATGGCAC TCCTGGGACC AAGCTGCAGC GCTTCAACTC CTACGACATT
    AGCAGAGATA CACTCTACGT CTCTAAGTGC ATCTGCCTCA TCACGCCCAT GTCCTTCATG
    AAAGCATGTC GGAGTGTGCT GGAACAGCTT CACCAGGCAG TCACTTCGCC TCAGCCCCCT
    CCACTGCCCC TTGAGAGCTA CATATACAAT GTACTATATG AGGTGCCACT CCCACCTCCT
    GGCCGGTCCT TGAAGTTTTC TGGTGTCTAT GGGCCAATCG TCTGCCAGAG ACCAAGTACC
    AATGAGCTTC CCCTGTTCGA CTTTCCTGTC AAAGAGGTCT TTGAACTGCT TGGGGTGGAG
    AATGTGTTTC AACTTTTTAC TTGTGCCCTT CTGGAGTTTC AAATCCTGCT CTACTCACAG
    CATTACCAGC GACTGATGAC TGTGGCGGAG ACAATTACAG CTCTCATGTT TCCTTTCCAG
    TGGCAGCATG TCTATGTTCC TATTCTCCCG GCTTCTCTCC TACATTTCTT AGATGCTCCT
    GTGCCATACT TGATGGGCTT GCATTCCAAT GGCTTGGATG ACCGGTCAAA ACTGGAGCTG
    CCTCAAGAGG CTAACCTCTG CTTTGTGGAC ATTGACAACC ACTTCATTGA GTTACCAGAG
    GACTTGCCTC AGTTTCCCAA CAAATTGGAG TTTGTCCAGG AAGTCTCTGA GATTCTCATG
    GCATTTGGCA TTCCCCCTGA AGGAAGCCTT CACTGTAGCG AGAGTGCTTC CAAGCTGAAG
    AGGCTCCGGG CCTCTGAACT CGTCTCTGAC AAGAGGAATG GAAACATCGC AGGCTCCCCT
    TTACATTCCT ATGAGTTCCT CAAGGAGAAT GAAACTATCG CCCGCCTGCA GGCCTTGGTC
    AAGAGGACTG GGGTGAGCCT GGAGAAGTTG GAAGTGCGTG AAGACCCCAG TAGCAATAAG
    GATCTCAAAG TTCAGTGTGA TGAAGAAGAA CTCAGGATTT ACCAGCTAAA CATTCAGATC
    CGGGAAGTTT TTGCAAATCG TTTCACTCAG ATGTTTGCAG ATTATGAGGT GTTTGTCATT
    CAGCCCAGCC AGGATAAGGA GTCCTGGTTT ACCAACAGAG AGCAGATGCA AAATTTTGAT
    AAAGCATCTT TTCTGTCAGA TCAGCCTGAA CCCTACCTGC CTTTCCTCTC AAGATTCTTG
    GAGACTCAGA TGTTTGCTTC TTTCATTGAC AACAAAATCA TGTGTCATGA CGATGATGAT
    AAAGATCCTG TGCTCCGGGT GTTTGACTCC CGAGTAGACA AGATCAGGCT GTTGAATGTT
    CGGACACCTA CTCTGCGCAC ATCCATGTAC CAGAAGTGTA CCACAGTGGA TGAAGCAAAG
    AAAGCAATTG AGCTGCGATT GGCAAAAATT GATCATACTG CGGTTCATCC CCATTTACTT
    GACATGAAGA TTGGACAAGG GAAGTATGAA CCAGGTTTCT TCCCTAAGCT ACAGTCTGAC
    GTGCTTTCCA CTGGGCCAGC CAGCAACAAG TGGACAAAAA GAAATGCTCC TGCCCAGTGG
    AGGCGTAAAG ACCGGCAGAA GCAGCACACA GAACACCTGC GCTTAGACAA TGACCAGAGA
    GAGAAGTACA TCCAGGAAGC CAGGAATATG GGCAGCACCA TCCGCCAGCC CAAACTGTCC
    AACCTCTCCC CGTCAGTGAT TGCCCAGACC AACTGGAAGT TTGTGGAAGG TCTGCTGAAG
    GAATGCCGCA ACAAGACCAA GAGGATGCTG GTGGAGAAGA TGGGGCGAGA GGCTGTGGAA
    CTAGGCCATG GGGAAGTGAA CATCACGGGG GTGGAAGAGA ACACCTTGAT TGCCAGTCTT
    TGTGACCTAC TGGAAAGGAT TTGGAGTCAT GGGCTACAGG TGAAACAGGG GAAATCAGCC
    TTGTGGTCCC ACCTGTTACA TTATCAGGAA AACCGGCAGA GAAAACTCAC ATCAGGAAGC
    CTCAGTACCT CAGGAATATT TCTTGATTCA GAGCGGAGGA AGTCTGATGC CAGCTCCGTC
    ATGTCTCCCC TGAAAATCTC CCTCATTCAG GATATGAGGC ACATCCAGAA CATTGGGGAA
    ATCAAGACTG ATGTGGGAAA AGCCAGAGCG TGGGTGCGTC TGTCCATGGA AAAAAAGTTA
    CTTTCTAGAC ACCTGAAGCA GCTTCTCTCA GACCATGAGC TCACCAAAAA ACTCTACAAG
    CGCTACGCCT TCCTGCGCTG TGACGACGAG AAGGAGCAGT TCCTCTATCA CCTCCTCTCT
    TTCAATGCCG TCGATTACTT CTGCTTCACC AATGTCTTCA CGACTATCCT GATTCCTTAC
    CACATCCTGA TTGTACCAAG CAAGAAGCTG GGGGGCTCTA TGTTCACCGC CAACCCATGG
    ATCTGCATCT CAGGAGAACT GGGTGAGACA CAGATCCTGC AGATTCCCAG GAACCTGTTA
    GAGATGACCT TCGAGTGCCA AAACTTGGGG AAACTCACTA CTGTGCAGAT AGGCCATGAT
    AACTCTGGAC TCTACGCCAA ATGGTTGGTG GAATATGTGA TGGTTAGGAA TGAGATCACC
    GGACATACCT ACAAGTTTCC ATGTGGCCGC TGGTTGGGGA AGGGCATGGA TGACGGGAGC
    CTGGAGCGGG TTCTGGTCGG GGAGCTGCTC ACATCCCAGC CTGAGGTGGA TGAGAGGCCG
    TGCCGGACTC CGCCCCTGCA GCAGTCTCCC AGCGTCATCC GGAGGCTTGT TACCATCTCA
    CCCAACAACA GACCCAAGCT GAACACTGGG CAGATCCAAG AGTCCATCGG TGAGGCAGTC
    AATGGCATCG TGAAGCACTT CCATAAGCCT GAAAAAGAGC GAGGTAGTCT GACGCTGTTG
    CTTTGTGGAG AATGTGGCCT TGTCTCGGCC CTGGAACAGG CTTTCCAGCA TGGATTTAAG
    TCACCACGGC TCTTCAAAAA TGTCTTCATT TGGGATTTTT TGGAAAAAGC ACAAACCTAT
    TATGAGACTT TGGAGCAGAA CGAACTTGTC CCTGAGGAAA ACTGGCATAT GAGGGCCCGG
    AACTTCTGCC GATTTGTCAC GGCAATCAAC AATAATCCCC GGAACATTGG CAAGGATGGC
    AAGTTTCAGA TGCTGGTGTG CTTGGGAGCC AGAGACCACC TCCTGCACCA CTGGATCGCC
    CTGCTGGCTG ACTGCCCCAT CACCGCACAC ATGTATGAGG ATGTGGCACT GATCAAAGAC
    CACACACTTG TCAATTCCTT GATCCGCGTG CTGCAGACAC TGCAGGAGTT CAACATCACG
    CTGGAGACAT CTCTGGTCAA GGGCATCGAT ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831576.1
    CDS198..3773
    Translation

    Target ORF information:

    RefSeq Version XM_024975808.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DENN domain containing 5A (DENND5A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975808.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTATGCA TGCCCAAAGG GCTGGCATTC AAGACTCAGG CTGATCCCAG GGAGCCTCAG 
    TTCCATGCCT TTATTATCAC TAGGGAGGAT GGCTCTCGGA CCTTTGGGTT TGCTCTCACG
    TTTTATGAGG AGGTGACTAG CAAGCAGATC TGCAGTGCAA TGCAGACCCT CTACCACATG
    CATAATGCAG AGTATGATGT CCTCCATGCT CCACTTGCTG ATAGCCGAGA CCTCAGTGGC
    ATGGAGGATG GTGATGGCAC TCCTGGGACC AAGCTGCAGC GCTTCAACTC CTACGACATT
    AGCAGAGATA CACTCTACGT CTCTAAGTGC ATCTGCCTCA TCACGCCCAT GTCCTTCATG
    AAAGCATGTC GGAGTGTGCT GGAACAGCTT CACCAGGCAG TCACTTCGCC TCAGCCCCCT
    CCACTGCCCC TTGAGAGCTA CATATACAAT GTACTATATG AGGTGCCACT CCCACCTCCT
    GGCCGGTCCT TGAAGTTTTC TGGTGTCTAT GGGCCAATCG TCTGCCAGAG ACCAAGTACC
    AATGAGCTTC CCCTGTTCGA CTTTCCTGTC AAAGAGGTCT TTGAACTGCT TGGGGTGGAG
    AATGTGTTTC AACTTTTTAC TTGTGCCCTT CTGGAGTTTC AAATCCTGCT CTACTCACAG
    CATTACCAGC GACTGATGAC TGTGGCGGAG ACAATTACAG CTCTCATGTT TCCTTTCCAG
    TGGCAGCATG TCTATGTTCC TATTCTCCCG GCTTCTCTCC TACATTTCTT AGATGCTCCT
    GTGCCATACT TGATGGGCTT GCATTCCAAT GGCTTGGATG ACCGGTCAAA ACTGGAGCTG
    CCTCAAGAGG CTAACCTCTG CTTTGTGGAC ATTGACAACC ACTTCATTGA GTTACCAGAG
    GACTTGCCTC AGTTTCCCAA CAAATTGGAG TTTGTCCAGG AAGTCTCTGA GATTCTCATG
    GCATTTGGCA TTCCCCCTGA AGGAAGCCTT CACTGTAGCG AGAGTGCTTC CAAGCTGAAG
    AGGCTCCGGG CCTCTGAACT CGTCTCTGAC AAGAGGAATG GAAACATCGC AGGCTCCCCT
    TTACATTCCT ATGAGTTCCT CAAGGAGAAT GAAACTATCG CCCGCCTGCA GGCCTTGGTC
    AAGAGGACTG GGGTGAGCCT GGAGAAGTTG GAAGTGCGTG AAGACCCCAG TAGCAATAAG
    GATCTCAAAG TTCAGTGTGA TGAAGAAGAA CTCAGGATTT ACCAGCTAAA CATTCAGATC
    CGGGAAGTTT TTGCAAATCG TTTCACTCAG ATGTTTGCAG ATTATGAGGT GTTTGTCATT
    CAGCCCAGCC AGGATAAGGA GTCCTGGTTT ACCAACAGAG AGCAGATGCA AAATTTTGAT
    AAAGCATCTT TTCTGTCAGA TCAGCCTGAA CCCTACCTGC CTTTCCTCTC AAGATTCTTG
    GAGACTCAGA TGTTTGCTTC TTTCATTGAC AACAAAATCA TGTGTCATGA CGATGATGAT
    AAAGATCCTG TGCTCCGGGT GTTTGACTCC CGAGTAGACA AGATCAGGCT GTTGAATGTT
    CGGACACCTA CTCTGCGCAC ATCCATGTAC CAGAAGTGTA CCACAGTGGA TGAAGCAAAG
    AAAGCAATTG AGCTGCGATT GGCAAAAATT GATCATACTG CGGTTCATCC CCATTTACTT
    GACATGAAGA TTGGACAAGG GAAGTATGAA CCAGGTTTCT TCCCTAAGCT ACAGTCTGAC
    GTGCTTTCCA CTGGGCCAGC CAGCAACAAG TGGACAAAAA GAAATGCTCC TGCCCAGTGG
    AGGCGTAAAG ACCGGCAGAA GCAGCACACA GAACACCTGC GCTTAGACAA TGACCAGAGA
    GAGAAGTACA TCCAGGAAGC CAGGAATATG GGCAGCACCA TCCGCCAGCC CAAACTGTCC
    AACCTCTCCC CGTCAGTGAT TGCCCAGACC AACTGGAAGT TTGTGGAAGG TCTGCTGAAG
    GAATGCCGCA ACAAGACCAA GAGGATGCTG GTGGAGAAGA TGGGGCGAGA GGCTGTGGAA
    CTAGGCCATG GGGAAGTGAA CATCACGGGG GTGGAAGAGA ACACCTTGAT TGCCAGTCTT
    TGTGACCTAC TGGAAAGGAT TTGGAGTCAT GGGCTACAGG TGAAACAGGG GAAATCAGCC
    TTGTGGTCCC ACCTGTTACA TTATCAGGAA AACCGGCAGA GAAAACTCAC ATCAGGAAGC
    CTCAGTACCT CAGGAATATT TCTTGATTCA GAGCGGAGGA AGTCTGATGC CAGCTCCGTC
    ATGTCTCCCC TGAAAATCTC CCTCATTCAG GATATGAGGC ACATCCAGAA CATTGGGGAA
    ATCAAGACTG ATGTGGGAAA AGCCAGAGCG TGGGTGCGTC TGTCCATGGA AAAAAAGTTA
    CTTTCTAGAC ACCTGAAGCA GCTTCTCTCA GACCATGAGC TCACCAAAAA ACTCTACAAG
    CGCTACGCCT TCCTGCGCTG TGACGACGAG AAGGAGCAGT TCCTCTATCA CCTCCTCTCT
    TTCAATGCCG TCGATTACTT CTGCTTCACC AATGTCTTCA CGACTATCCT GATTCCTTAC
    CACATCCTGA TTGTACCAAG CAAGAAGCTG GGGGGCTCTA TGTTCACCGC CAACCCATGG
    ATCTGCATCT CAGGAGAACT GGGTGAGACA CAGATCCTGC AGATTCCCAG GAACCTGTTA
    GAGATGACCT TCGAGTGCCA AAACTTGGGG AAACTCACTA CTGTGCAGAT AGGCCATGAT
    AACTCTGGAC TCTACGCCAA ATGGTTGGTG GAATATGTGA TGGTTAGGAA TGAGATCACC
    GGACATACCT ACAAGTTTCC ATGTGGCCGC TGGTTGGGGA AGGGCATGGA TGACGGGAGC
    CTGGAGCGGG TTCTGGTCGG GGAGCTGCTC ACATCCCAGC CTGAGGTGGA TGAGAGGCCG
    TGCCGGACTC CGCCCCTGCA GCAGTCTCCC AGCGTCATCC GGAGGCTTGT TACCATCTCA
    CCCAACAACA GACCCAAGCT GAACACTGGG CAGATCCAAG AGTCCATCGG TGAGGCAGTC
    AATGGCATCG TGAAGCACTT CCATAAGCCT GAAAAAGAGC GAGGTAGTCT GACGCTGTTG
    CTTTGTGGAG AATGTGGCCT TGTCTCGGCC CTGGAACAGG CTTTCCAGCA TGGATTTAAG
    TCACCACGGC TCTTCAAAAA TGTCTTCATT TGGGATTTTT TGGAAAAAGC ACAAACCTAT
    TATGAGACTT TGGAGCAGAA CGAACTTGTC CCTGAGGAAA ACTGGCATAT GAGGGCCCGG
    AACTTCTGCC GATTTGTCAC GGCAATCAAC AATAATCCCC GGAACATTGG CAAGGATGGC
    AAGTTTCAGA TGCTGGTGTG CTTGGGAGCC AGAGACCACC TCCTGCACCA CTGGATCGCC
    CTGCTGGCTG ACTGCCCCAT CACCGCACAC ATGTATGAGG ATGTGGCACT GATCAAAGAC
    CACACACTTG TCAATTCCTT GATCCGCGTG CTGCAGACAC TGCAGGAGTT CAACATCACG
    CTGGAGACAT CTCTGGTCAA GGGCATCGAT ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223235
    Clone ID Related Accession (Same CDS sequence) XM_005216096.4
    Accession Version XM_005216096.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3735bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DENN domain-containing protein 5A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216096.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGCGGCG GCGGCGGCGG CGGCGGCGGA GGGGGCTCGG CGCCCAGTCG CTTCGCCGAC 
    TACTTTGTCA TCTGCGGGCT GGACACCGAG ACCGGGCTGG AGCCGGACGA GCTGTCGGCA
    TTATGCCAGT ACATACAGGC TTCTAAAGCC AGGGATGGTG CCAGCTCTTT CATTTCAAGT
    ACAACTGAAG GAGAAAATTT TGAGCAGACA CCGTTGAGAC GAACATTCAA GTCTAAGGTC
    CTTGCACGAT ATCCTGAGAA TGTGGACTGG AATCCCTTTG ACCAAGATGC AGTGGGAATG
    CTATGCATGC CCAAAGGGCT GGCATTCAAG ACTCAGGCTG ATCCCAGGGA GCCTCAGTTC
    CATGCCTTTA TTATCACTAG GGAGGATGGC TCTCGGACCT TTGGGTTTGC TCTCACGTTT
    TATGAGGAGG TGACTAGCAA GCAGATCTGC AGTGCAATGC AGACCCTCTA CCACATGCAT
    AATGCAGAGT ATGATGTCCT CCATGCTCCA CTTGCTGATA GCCGAGACCT CAGTGGCATG
    GAGGATGGTG ATGGCACTCC TGGGACCAAG CTGCAGCGCT TCAACTCCTA CGACATTAGC
    AGAGATACAC TCTACGTCTC TAAGTGCATC TGCCTCATCA CGCCCATGTC CTTCATGAAA
    GCATGTCGGA GTGTGCTGGA ACAGCTTCAC CAGGCAGTCA CTTCGCCTCA GCCCCCTCCA
    CTGCCCCTTG AGAGCTACAT ATACAATGTA CTATATGAGG TGCCACTCCC ACCTCCTGGC
    CGGTCCTTGA AGTTTTCTGG TGTCTATGGG CCAATCGTCT GCCAGAGACC AAGTACCAAT
    GAGCTTCCCC TGTTCGACTT TCCTGTCAAA GAGGTCTTTG AACTGCTTGG GGTGGAGAAT
    GTGTTTCAAC TTTTTACTTG TGCCCTTCTG GAGTTTCAAA TCCTGCTCTA CTCACAGCAT
    TACCAGCGAC TGATGACTGT GGCGGAGACA ATTACAGCTC TCATGTTTCC TTTCCAGTGG
    CAGCATGTCT ATGTTCCTAT TCTCCCGGCT TCTCTCCTAC ATTTCTTAGA TGCTCCTGTG
    CCATACTTGA TGGGCTTGCA TTCCAATGGC TTGGATGACC GGTCAAAACT GGAGCTGCCT
    CAAGAGGCTA ACCTCTGCTT TGTGGACATT GACAACCACT TCATTGAGTT ACCAGAGGAC
    TTGCCTCAGT TTCCCAACAA ATTGGAGTTT GTCCAGGAAG TCTCTGAGAT TCTCATGGCA
    TTTGGCATTC CCCCTGAAGG AAGCCTTCAC TGTAGCGAGA GTGCTTCCAA GCTGAAGAGG
    CTCCGGGCCT CTGAACTCGT CTCTGACAAG AGGAATGGAA ACATCGCAGG CTCCCCTTTA
    CATTCCTATG AGTTCCTCAA GGAGAATGAA ACTATCGCCC GCCTGCAGGC CTTGGTCAAG
    AGGACTGGGG TGAGCCTGGA GAAGTTGGAA GTGCGTGAAG ACCCCAGTAG CAATAAGGAT
    CTCAAAGTTC AGTGTGATGA AGAAGAACTC AGGATTTACC AGCTAAACAT TCAGATCCGG
    GAAGTTTTTG CAAATCGTTT CACTCAGATG TTTGCAGATT ATGAGGTGTT TGTCATTCAG
    CCCAGCCAGG ATAAGGAGTC CTGGTTTACC AACAGAGAGC AGATGCAAAA TTTTGATAAA
    GCATCTTTTC TGTCAGATCA GCCTGAACCC TACCTGCCTT TCCTCTCAAG ATTCTTGGAG
    ACTCAGATGT TTGCTTCTTT CATTGACAAC AAAATCATGT GTCATGACGA TGATGATAAA
    GATCCTGTGC TCCGGGTGTT TGACTCCCGA GTAGACAAGA TCAGGCTGTT GAATGTTCGG
    ACACCTACTC TGCGCACATC CATGTACCAG AAGTGTACCA CAGTGGATGA AGCAAAGAAA
    GCAATTGAGC TGCGATTGGC AAAAATTGAT CATACTGCGG TTCATCCCCA TTTACTTGAC
    ATGAAGATTG GACAAGGGAA GTATGAACCA GGTTTCTTCC CTAAGCTACA GTCTGACGTG
    CTTTCCACTG GGCCAGCCAG CAACAAGTGG ACAAAAAGAA ATGCTCCTGC CCAGTGGAGG
    CGTAAAGACC GGCAGAAGCA GCACACAGAA CACCTGCGCT TAGACAATGA CCAGAGAGAG
    AAGTACATCC AGGAAGCCAG GAATATGGGC AGCACCATCC GCCAGCCCAA ACTGTCCAAC
    CTCTCCCCGT CAGTGATTGC CCAGACCAAC TGGAAGTTTG TGGAAGGTCT GCTGAAGGAA
    TGCCGCAACA AGACCAAGAG GATGCTGGTG GAGAAGATGG GGCGAGAGGC TGTGGAACTA
    GGCCATGGGG AAGTGAACAT CACGGGGGTG GAAGAGAACA CCTTGATTGC CAGTCTTTGT
    GACCTACTGG AAAGGATTTG GAGTCATGGG CTACAGGTGA AACAGGGGAA ATCAGCCTTG
    TGGTCCCACC TGTTACATTA TCAGGAAAAC CGGCAGAGAA AACTCACATC AGGAAGCCTC
    AGTACCTCAG GAATATTTCT TGATTCAGAG CGGAGGAAGT CTGATGCCAG CTCCGTCATG
    TCTCCCCTGA AAATCTCCCT CATTCAGGAT ATGAGGCACA TCCAGAACAT TGGGGAAATC
    AAGACTGATG TGGGAAAAGC CAGAGCGTGG GTGCGTCTGT CCATGGAAAA AAAGTTACTT
    TCTAGACACC TGAAGCAGCT TCTCTCAGAC CATGAGCTCA CCAAAAAACT CTACAAGCGC
    TACGCCTTCC TGCGCTGTGA CGACGAGAAG GAGCAGTTCC TCTATCACCT CCTCTCTTTC
    AATGCCGTCG ATTACTTCTG CTTCACCAAT GTCTTCACGA CTATCCTGAT TCCTTACCAC
    ATCCTGATTG TACCAAGCAA GAAGCTGGGG GGCTCTATGT TCACCGCCAA CCCATGGATC
    TGCATCTCAG GAGAACTGGG TGAGACACAG ATCCTGCAGA TTCCCAGGAA CCTGTTAGAG
    ATGACCTTCG AGTGCCAAAA CTTGGGGAAA CTCACTACTG TGCAGATAGG CCATGATAAC
    TCTGGACTCT ACGCCAAATG GTTGGTGGAA TATGTGATGG TTAGGAATGA GATCACCGGA
    CATACCTACA AGTTTCCATG TGGCCGCTGG TTGGGGAAGG GCATGGATGA CGGGAGCCTG
    GAGCGGGTTC TGGTCGGGGA GCTGCTCACA TCCCAGCCTG AGGTGGATGA GAGGCCGTGC
    CGGACTCCGC CCCTGCAGCA GTCTCCCAGC GTCATCCGGA GGCTTGTTAC CATCTCACCC
    AACAACAGAC CCAAGCTGAA CACTGGGCAG ATCCAAGAGT CCATCGGTGA GGCAGTCAAT
    GGCATCGTGA AGCACTTCCA TAAGCCTGAA AAAGAGCGAG GTAGTCTGAC GCTGTTGCTT
    TGTGGAGAAT GTGGCCTTGT CTCGGCCCTG GAACAGGCTT TCCAGCATGG ATTTAAGTCA
    CCACGGCTCT TCAAAAATGT CTTCATTTGG GATTTTTTGG AAAAAGCACA AACCTATTAT
    GAGACTTTGG AGCAGAACGA ACTTGTCCCT GAGGAAAACT GGCATATGAG GGCCCGGAAC
    TTCTGCCGAT TTGTCACGGC AATCAACAAT AATCCCCGGA ACATTGGCAA GGATGAGACC
    ACCTCCTGCA CCACTGGATC GCCCTGCTGG CTGACTGCCC CATCACCGCA CACATGTATG
    AGGATGTGGC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216153.2
    CDS35..3769
    Translation

    Target ORF information:

    RefSeq Version XM_005216096.4
    Organism Bos taurus(cattle)
    Definition Bos taurus DENN domain containing 5A (DENND5A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216096.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGCGGCG GCGGCGGCGG CGGCGGCGGA GGGGGCTCGG CGCCCAGTCG CTTCGCCGAC 
    TACTTTGTCA TCTGCGGGCT GGACACCGAG ACCGGGCTGG AGCCGGACGA GCTGTCGGCA
    TTATGCCAGT ACATACAGGC TTCTAAAGCC AGGGATGGTG CCAGCTCTTT CATTTCAAGT
    ACAACTGAAG GAGAAAATTT TGAGCAGACA CCGTTGAGAC GAACATTCAA GTCTAAGGTC
    CTTGCACGAT ATCCTGAGAA TGTGGACTGG AATCCCTTTG ACCAAGATGC AGTGGGAATG
    CTATGCATGC CCAAAGGGCT GGCATTCAAG ACTCAGGCTG ATCCCAGGGA GCCTCAGTTC
    CATGCCTTTA TTATCACTAG GGAGGATGGC TCTCGGACCT TTGGGTTTGC TCTCACGTTT
    TATGAGGAGG TGACTAGCAA GCAGATCTGC AGTGCAATGC AGACCCTCTA CCACATGCAT
    AATGCAGAGT ATGATGTCCT CCATGCTCCA CTTGCTGATA GCCGAGACCT CAGTGGCATG
    GAGGATGGTG ATGGCACTCC TGGGACCAAG CTGCAGCGCT TCAACTCCTA CGACATTAGC
    AGAGATACAC TCTACGTCTC TAAGTGCATC TGCCTCATCA CGCCCATGTC CTTCATGAAA
    GCATGTCGGA GTGTGCTGGA ACAGCTTCAC CAGGCAGTCA CTTCGCCTCA GCCCCCTCCA
    CTGCCCCTTG AGAGCTACAT ATACAATGTA CTATATGAGG TGCCACTCCC ACCTCCTGGC
    CGGTCCTTGA AGTTTTCTGG TGTCTATGGG CCAATCGTCT GCCAGAGACC AAGTACCAAT
    GAGCTTCCCC TGTTCGACTT TCCTGTCAAA GAGGTCTTTG AACTGCTTGG GGTGGAGAAT
    GTGTTTCAAC TTTTTACTTG TGCCCTTCTG GAGTTTCAAA TCCTGCTCTA CTCACAGCAT
    TACCAGCGAC TGATGACTGT GGCGGAGACA ATTACAGCTC TCATGTTTCC TTTCCAGTGG
    CAGCATGTCT ATGTTCCTAT TCTCCCGGCT TCTCTCCTAC ATTTCTTAGA TGCTCCTGTG
    CCATACTTGA TGGGCTTGCA TTCCAATGGC TTGGATGACC GGTCAAAACT GGAGCTGCCT
    CAAGAGGCTA ACCTCTGCTT TGTGGACATT GACAACCACT TCATTGAGTT ACCAGAGGAC
    TTGCCTCAGT TTCCCAACAA ATTGGAGTTT GTCCAGGAAG TCTCTGAGAT TCTCATGGCA
    TTTGGCATTC CCCCTGAAGG AAGCCTTCAC TGTAGCGAGA GTGCTTCCAA GCTGAAGAGG
    CTCCGGGCCT CTGAACTCGT CTCTGACAAG AGGAATGGAA ACATCGCAGG CTCCCCTTTA
    CATTCCTATG AGTTCCTCAA GGAGAATGAA ACTATCGCCC GCCTGCAGGC CTTGGTCAAG
    AGGACTGGGG TGAGCCTGGA GAAGTTGGAA GTGCGTGAAG ACCCCAGTAG CAATAAGGAT
    CTCAAAGTTC AGTGTGATGA AGAAGAACTC AGGATTTACC AGCTAAACAT TCAGATCCGG
    GAAGTTTTTG CAAATCGTTT CACTCAGATG TTTGCAGATT ATGAGGTGTT TGTCATTCAG
    CCCAGCCAGG ATAAGGAGTC CTGGTTTACC AACAGAGAGC AGATGCAAAA TTTTGATAAA
    GCATCTTTTC TGTCAGATCA GCCTGAACCC TACCTGCCTT TCCTCTCAAG ATTCTTGGAG
    ACTCAGATGT TTGCTTCTTT CATTGACAAC AAAATCATGT GTCATGACGA TGATGATAAA
    GATCCTGTGC TCCGGGTGTT TGACTCCCGA GTAGACAAGA TCAGGCTGTT GAATGTTCGG
    ACACCTACTC TGCGCACATC CATGTACCAG AAGTGTACCA CAGTGGATGA AGCAAAGAAA
    GCAATTGAGC TGCGATTGGC AAAAATTGAT CATACTGCGG TTCATCCCCA TTTACTTGAC
    ATGAAGATTG GACAAGGGAA GTATGAACCA GGTTTCTTCC CTAAGCTACA GTCTGACGTG
    CTTTCCACTG GGCCAGCCAG CAACAAGTGG ACAAAAAGAA ATGCTCCTGC CCAGTGGAGG
    CGTAAAGACC GGCAGAAGCA GCACACAGAA CACCTGCGCT TAGACAATGA CCAGAGAGAG
    AAGTACATCC AGGAAGCCAG GAATATGGGC AGCACCATCC GCCAGCCCAA ACTGTCCAAC
    CTCTCCCCGT CAGTGATTGC CCAGACCAAC TGGAAGTTTG TGGAAGGTCT GCTGAAGGAA
    TGCCGCAACA AGACCAAGAG GATGCTGGTG GAGAAGATGG GGCGAGAGGC TGTGGAACTA
    GGCCATGGGG AAGTGAACAT CACGGGGGTG GAAGAGAACA CCTTGATTGC CAGTCTTTGT
    GACCTACTGG AAAGGATTTG GAGTCATGGG CTACAGGTGA AACAGGGGAA ATCAGCCTTG
    TGGTCCCACC TGTTACATTA TCAGGAAAAC CGGCAGAGAA AACTCACATC AGGAAGCCTC
    AGTACCTCAG GAATATTTCT TGATTCAGAG CGGAGGAAGT CTGATGCCAG CTCCGTCATG
    TCTCCCCTGA AAATCTCCCT CATTCAGGAT ATGAGGCACA TCCAGAACAT TGGGGAAATC
    AAGACTGATG TGGGAAAAGC CAGAGCGTGG GTGCGTCTGT CCATGGAAAA AAAGTTACTT
    TCTAGACACC TGAAGCAGCT TCTCTCAGAC CATGAGCTCA CCAAAAAACT CTACAAGCGC
    TACGCCTTCC TGCGCTGTGA CGACGAGAAG GAGCAGTTCC TCTATCACCT CCTCTCTTTC
    AATGCCGTCG ATTACTTCTG CTTCACCAAT GTCTTCACGA CTATCCTGAT TCCTTACCAC
    ATCCTGATTG TACCAAGCAA GAAGCTGGGG GGCTCTATGT TCACCGCCAA CCCATGGATC
    TGCATCTCAG GAGAACTGGG TGAGACACAG ATCCTGCAGA TTCCCAGGAA CCTGTTAGAG
    ATGACCTTCG AGTGCCAAAA CTTGGGGAAA CTCACTACTG TGCAGATAGG CCATGATAAC
    TCTGGACTCT ACGCCAAATG GTTGGTGGAA TATGTGATGG TTAGGAATGA GATCACCGGA
    CATACCTACA AGTTTCCATG TGGCCGCTGG TTGGGGAAGG GCATGGATGA CGGGAGCCTG
    GAGCGGGTTC TGGTCGGGGA GCTGCTCACA TCCCAGCCTG AGGTGGATGA GAGGCCGTGC
    CGGACTCCGC CCCTGCAGCA GTCTCCCAGC GTCATCCGGA GGCTTGTTAC CATCTCACCC
    AACAACAGAC CCAAGCTGAA CACTGGGCAG ATCCAAGAGT CCATCGGTGA GGCAGTCAAT
    GGCATCGTGA AGCACTTCCA TAAGCCTGAA AAAGAGCGAG GTAGTCTGAC GCTGTTGCTT
    TGTGGAGAAT GTGGCCTTGT CTCGGCCCTG GAACAGGCTT TCCAGCATGG ATTTAAGTCA
    CCACGGCTCT TCAAAAATGT CTTCATTTGG GATTTTTTGG AAAAAGCACA AACCTATTAT
    GAGACTTTGG AGCAGAACGA ACTTGTCCCT GAGGAAAACT GGCATATGAG GGCCCGGAAC
    TTCTGCCGAT TTGTCACGGC AATCAACAAT AATCCCCGGA ACATTGGCAA GGATGAGACC
    ACCTCCTGCA CCACTGGATC GCCCTGCTGG CTGACTGCCC CATCACCGCA CACATGTATG
    AGGATGTGGC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26409
    Clone ID Related Accession (Same CDS sequence) XM_002693106.4 , XM_001252217.6
    Accession Version XM_002693106.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DENN domain-containing protein 5A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_002693106.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCGGCG GCGGCGGCGG CGGCGGAGGG GGCTCGGCGC CCAGTCGCTT CGCCGACTAC 
    TTTGTCATCT GCGGGCTGGA CACCGAGACC GGGCTGGAGC CGGACGAGCT GTCGGCATTA
    TGCCAGTACA TACAGGCTTC TAAAGCCAGG GATGGTGCCA GCTCTTTCAT TTCAAGTACA
    ACTGAAGGAG AAAATTTTGA GCAGACACCG TTGAGACGAA CATTCAAGTC TAAGGTCCTT
    GCACGATATC CTGAGAATGT GGACTGGAAT CCCTTTGACC AAGATGCAGT GGGAATGCTA
    TGCATGCCCA AAGGGCTGGC ATTCAAGACT CAGGCTGATC CCAGGGAGCC TCAGTTCCAT
    GCCTTTATTA TCACTAGGGA GGATGGCTCT CGGACCTTTG GGTTTGCTCT CACGTTTTAT
    GAGGAGGTGA CTAGCAAGCA GATCTGCAGT GCAATGCAGA CCCTCTACCA CATGCATAAT
    GCAGAGTATG ATGTCCTCCA TGCTCCACTT GCTGATAGCC GAGACCTCAG TGGCATGGAG
    GATGGTGATG GCACTCCTGG GACCAAGCTG CAGCGCTTCA ACTCCTACGA CATTAGCAGA
    GATACACTCT ACGTCTCTAA GTGCATCTGC CTCATCACGC CCATGTCCTT CATGAAAGCA
    TGTCGGAGTG TGCTGGAACA GCTTCACCAG GCAGTCACTT CGCCTCAGCC CCCTCCACTG
    CCCCTTGAGA GCTACATATA CAATGTACTA TATGAGGTGC CACTCCCACC TCCTGGCCGG
    TCCTTGAAGT TTTCTGGTGT CTATGGGCCA ATCGTCTGCC AGAGACCAAG TACCAATGAG
    CTTCCCCTGT TCGACTTTCC TGTCAAAGAG GTCTTTGAAC TGCTTGGGGT GGAGAATGTG
    TTTCAACTTT TTACTTGTGC CCTTCTGGAG TTTCAAATCC TGCTCTACTC ACAGCATTAC
    CAGCGACTGA TGACTGTGGC GGAGACAATT ACAGCTCTCA TGTTTCCTTT CCAGTGGCAG
    CATGTCTATG TTCCTATTCT CCCGGCTTCT CTCCTACATT TCTTAGATGC TCCTGTGCCA
    TACTTGATGG GCTTGCATTC CAATGGCTTG GATGACCGGT CAAAACTGGA GCTGCCTCAA
    GAGGCTAACC TCTGCTTTGT GGACATTGAC AACCACTTCA TTGAGTTACC AGAGGACTTG
    CCTCAGTTTC CCAACAAATT GGAGTTTGTC CAGGAAGTCT CTGAGATTCT CATGGCATTT
    GGCATTCCCC CTGAAGGAAG CCTTCACTGT AGCGAGAGTG CTTCCAAGCT GAAGAGGCTC
    CGGGCCTCTG AACTCGTCTC TGACAAGAGG AATGGAAACA TCGCAGGCTC CCCTTTACAT
    TCCTATGAGT TCCTCAAGGA GAATGAAACT ATCGCCCGCC TGCAGGCCTT GGTCAAGAGG
    ACTGGGGTGA GCCTGGAGAA GTTGGAAGTG CGTGAAGACC CCAGTAGCAA TAAGGATCTC
    AAAGTTCAGT GTGATGAAGA AGAACTCAGG ATTTACCAGC TAAACATTCA GATCCGGGAA
    GTTTTTGCAA ATCGTTTCAC TCAGATGTTT GCAGATTATG AGGTGTTTGT CATTCAGCCC
    AGCCAGGATA AGGAGTCCTG GTTTACCAAC AGAGAGCAGA TGCAAAATTT TGATAAAGCA
    TCTTTTCTGT CAGATCAGCC TGAACCCTAC CTGCCTTTCC TCTCAAGATT CTTGGAGACT
    CAGATGTTTG CTTCTTTCAT TGACAACAAA ATCATGTGTC ATGACGATGA TGATAAAGAT
    CCTGTGCTCC GGGTGTTTGA CTCCCGAGTA GACAAGATCA GGCTGTTGAA TGTTCGGACA
    CCTACTCTGC GCACATCCAT GTACCAGAAG TGTACCACAG TGGATGAAGC AAAGAAAGCA
    ATTGAGCTGC GATTGGCAAA AATTGATCAT ACTGCGGTTC ATCCCCATTT ACTTGACATG
    AAGATTGGAC AAGGGAAGTA TGAACCAGGC TTCTTCCCTA AGCTACAGTC TGATGTGCTT
    TCCACTGGGC CAGCCAGCAA CAAGTGGACA AAAAGAAATG CTCCTGCCCA GTGGAGGCGT
    AAAGACCGGC AGAAGCAGCA CACAGAACAC CTGCGCTTAG ACAATGACCA GAGAGAGAAG
    TACATCCAGG AAGCCAGGAA TATGGGCAGC ACCATCCGCC AGCCCAAACT GTCCAACCTC
    TCCCCGTCAG TGATTGCCCA GACCAACTGG AAGTTTGTGG AAGGTCTGCT GAAGGAATGC
    CGCAACAAGA CCAAGAGGAT GCTGGTGGAG AAGATGGGGC GAGAGGCTGT GGAACTAGGC
    CATGGGGAAG TGAACATCAC AGGGGTGGAA GAGAACACCT TGATTGCCAG TCTTTGTGAC
    CTACTGGAAA GGATTTGGAG TCATGGGCTA CAGGTGAAAC AGGGGAAATC AGCCTTGTGG
    TCCCACCTGT TACATTATCA GGAAAACCGG CAGAGAAAAC TCACATCAGG AAGCCTCAGT
    ACCTCAGGAA TATTTCTTGA TTCAGAGCGG AGGAAGTCTG ATGCCAGCTC CGTCATGTCT
    CCCCTGAAAA TCTCCCTCAT TCAGGATATG AGGCACATCC AGAACATTGG GGAAATCAAG
    ACTGATGTGG GAAAAGCCAG AGCGTGGGTG CGTCTGTCCA TGGAAAAAAA GTTACTTTCT
    AGACACCTGA AGCAGCTTCT CTCAGACCAT GAGCTCACCA AAAAACTCTA CAAGCGCTAC
    GCCTTCCTGC GCTGTGACGA CGAGAAGGAG CAGTTCCTCT ATCACCTCCT CTCTTTCAAT
    GCCGTCGATT ACTTCTGCTT CACCAATGTC TTCACGACTA TCCTGATTCC TTACCACATC
    CTGATTGTAC CAAGCAAGAA GCTGGGGGGC TCTATGTTCA CCGCCAACCC ATGGATCTGC
    ATCTCAGGAG AACTGGGTGA GACACAGATC CTGCAGATTC CCAGGAACCT GTTAGAGATG
    ACCTTCGAGT GCCAAAACTT GGGGAAACTC ACTACTGTGC AGATAGGCCA TGATAACTCT
    GGACTCTACG CCAAATGGTT GGTGGAATAT GTGATGGTTA GGAATGAGAT CACCGGACAT
    ACCTACAAGT TTCCATGTGG CCGCTGGTTG GGGAAGGGCA TGGATGACGG GAGCCTGGAG
    CGGGTTCTGG TCGGGGAGCT GCTCACATCC CAGCCTGAGG TGGATGAGAG GCCGTGCCGG
    ACTCCGCCCC TGCAGCAGTC TCCCAGCGTC ATCCGGAGGC TTGTTACCAT CTCACCCAAC
    AACAGACCCA AGCTGAACAC TGGGCAGATC CAAGAGTCCA TCGGTGAGGC AGTCAATGGC
    ATCGTGAAGC ACTTCCATAA GCCTGAAAAA GAGCGAGGTA GTCTGACGCT GTTGCTTTGT
    GGAGAATGTG GCCTTGTCTC GGCCCTGGAA CAGGCTTTCC AGCATGGATT TAAGTCACCA
    CGGCTCTTCA AAAATGTCTT CATTTGGGAT TTTTTGGAAA AAGCACAAAC CTATTATGAG
    ACTTTGGAGC AGAACGAACT TGTCCCTGAG GAAAACTGGC ATATGAGGGC CCGGAACTTC
    TGCCGATTTG TCACGGCAAT CAACAATAAT CCCCGGAACA TTGGCAAGGA TGGCAAGTTT
    CAGATGCTGG TGTGCTTGGG AGCCAGAGAC CACCTCCTGC ACCACTGGAT CGCCCTGCTG
    GCTGACTGCC CCATCACCGC ACACATGTAT GAGGATGTGG CACTGATCAA AGACCACACA
    CTTGTCAATT CCTTGATCCG CGTGCTGCAG ACACTGCAGG AGTTCAACAT CACGCTGGAG
    ACATCTCTGG TCAAGGGCAT CGATATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002693152.1
    CDS68..3937
    Translation

    Target ORF information:

    RefSeq Version XM_002693106.4
    Organism Bos taurus(cattle)
    Definition Bos taurus DENN/MADD domain containing 5A (DENND5A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002693106.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCGGCG GCGGCGGCGG CGGCGGAGGG GGCTCGGCGC CCAGTCGCTT CGCCGACTAC 
    TTTGTCATCT GCGGGCTGGA CACCGAGACC GGGCTGGAGC CGGACGAGCT GTCGGCATTA
    TGCCAGTACA TACAGGCTTC TAAAGCCAGG GATGGTGCCA GCTCTTTCAT TTCAAGTACA
    ACTGAAGGAG AAAATTTTGA GCAGACACCG TTGAGACGAA CATTCAAGTC TAAGGTCCTT
    GCACGATATC CTGAGAATGT GGACTGGAAT CCCTTTGACC AAGATGCAGT GGGAATGCTA
    TGCATGCCCA AAGGGCTGGC ATTCAAGACT CAGGCTGATC CCAGGGAGCC TCAGTTCCAT
    GCCTTTATTA TCACTAGGGA GGATGGCTCT CGGACCTTTG GGTTTGCTCT CACGTTTTAT
    GAGGAGGTGA CTAGCAAGCA GATCTGCAGT GCAATGCAGA CCCTCTACCA CATGCATAAT
    GCAGAGTATG ATGTCCTCCA TGCTCCACTT GCTGATAGCC GAGACCTCAG TGGCATGGAG
    GATGGTGATG GCACTCCTGG GACCAAGCTG CAGCGCTTCA ACTCCTACGA CATTAGCAGA
    GATACACTCT ACGTCTCTAA GTGCATCTGC CTCATCACGC CCATGTCCTT CATGAAAGCA
    TGTCGGAGTG TGCTGGAACA GCTTCACCAG GCAGTCACTT CGCCTCAGCC CCCTCCACTG
    CCCCTTGAGA GCTACATATA CAATGTACTA TATGAGGTGC CACTCCCACC TCCTGGCCGG
    TCCTTGAAGT TTTCTGGTGT CTATGGGCCA ATCGTCTGCC AGAGACCAAG TACCAATGAG
    CTTCCCCTGT TCGACTTTCC TGTCAAAGAG GTCTTTGAAC TGCTTGGGGT GGAGAATGTG
    TTTCAACTTT TTACTTGTGC CCTTCTGGAG TTTCAAATCC TGCTCTACTC ACAGCATTAC
    CAGCGACTGA TGACTGTGGC GGAGACAATT ACAGCTCTCA TGTTTCCTTT CCAGTGGCAG
    CATGTCTATG TTCCTATTCT CCCGGCTTCT CTCCTACATT TCTTAGATGC TCCTGTGCCA
    TACTTGATGG GCTTGCATTC CAATGGCTTG GATGACCGGT CAAAACTGGA GCTGCCTCAA
    GAGGCTAACC TCTGCTTTGT GGACATTGAC AACCACTTCA TTGAGTTACC AGAGGACTTG
    CCTCAGTTTC CCAACAAATT GGAGTTTGTC CAGGAAGTCT CTGAGATTCT CATGGCATTT
    GGCATTCCCC CTGAAGGAAG CCTTCACTGT AGCGAGAGTG CTTCCAAGCT GAAGAGGCTC
    CGGGCCTCTG AACTCGTCTC TGACAAGAGG AATGGAAACA TCGCAGGCTC CCCTTTACAT
    TCCTATGAGT TCCTCAAGGA GAATGAAACT ATCGCCCGCC TGCAGGCCTT GGTCAAGAGG
    ACTGGGGTGA GCCTGGAGAA GTTGGAAGTG CGTGAAGACC CCAGTAGCAA TAAGGATCTC
    AAAGTTCAGT GTGATGAAGA AGAACTCAGG ATTTACCAGC TAAACATTCA GATCCGGGAA
    GTTTTTGCAA ATCGTTTCAC TCAGATGTTT GCAGATTATG AGGTGTTTGT CATTCAGCCC
    AGCCAGGATA AGGAGTCCTG GTTTACCAAC AGAGAGCAGA TGCAAAATTT TGATAAAGCA
    TCTTTTCTGT CAGATCAGCC TGAACCCTAC CTGCCTTTCC TCTCAAGATT CTTGGAGACT
    CAGATGTTTG CTTCTTTCAT TGACAACAAA ATCATGTGTC ATGACGATGA TGATAAAGAT
    CCTGTGCTCC GGGTGTTTGA CTCCCGAGTA GACAAGATCA GGCTGTTGAA TGTTCGGACA
    CCTACTCTGC GCACATCCAT GTACCAGAAG TGTACCACAG TGGATGAAGC AAAGAAAGCA
    ATTGAGCTGC GATTGGCAAA AATTGATCAT ACTGCGGTTC ATCCCCATTT ACTTGACATG
    AAGATTGGAC AAGGGAAGTA TGAACCAGGC TTCTTCCCTA AGCTACAGTC TGATGTGCTT
    TCCACTGGGC CAGCCAGCAA CAAGTGGACA AAAAGAAATG CTCCTGCCCA GTGGAGGCGT
    AAAGACCGGC AGAAGCAGCA CACAGAACAC CTGCGCTTAG ACAATGACCA GAGAGAGAAG
    TACATCCAGG AAGCCAGGAA TATGGGCAGC ACCATCCGCC AGCCCAAACT GTCCAACCTC
    TCCCCGTCAG TGATTGCCCA GACCAACTGG AAGTTTGTGG AAGGTCTGCT GAAGGAATGC
    CGCAACAAGA CCAAGAGGAT GCTGGTGGAG AAGATGGGGC GAGAGGCTGT GGAACTAGGC
    CATGGGGAAG TGAACATCAC AGGGGTGGAA GAGAACACCT TGATTGCCAG TCTTTGTGAC
    CTACTGGAAA GGATTTGGAG TCATGGGCTA CAGGTGAAAC AGGGGAAATC AGCCTTGTGG
    TCCCACCTGT TACATTATCA GGAAAACCGG CAGAGAAAAC TCACATCAGG AAGCCTCAGT
    ACCTCAGGAA TATTTCTTGA TTCAGAGCGG AGGAAGTCTG ATGCCAGCTC CGTCATGTCT
    CCCCTGAAAA TCTCCCTCAT TCAGGATATG AGGCACATCC AGAACATTGG GGAAATCAAG
    ACTGATGTGG GAAAAGCCAG AGCGTGGGTG CGTCTGTCCA TGGAAAAAAA GTTACTTTCT
    AGACACCTGA AGCAGCTTCT CTCAGACCAT GAGCTCACCA AAAAACTCTA CAAGCGCTAC
    GCCTTCCTGC GCTGTGACGA CGAGAAGGAG CAGTTCCTCT ATCACCTCCT CTCTTTCAAT
    GCCGTCGATT ACTTCTGCTT CACCAATGTC TTCACGACTA TCCTGATTCC TTACCACATC
    CTGATTGTAC CAAGCAAGAA GCTGGGGGGC TCTATGTTCA CCGCCAACCC ATGGATCTGC
    ATCTCAGGAG AACTGGGTGA GACACAGATC CTGCAGATTC CCAGGAACCT GTTAGAGATG
    ACCTTCGAGT GCCAAAACTT GGGGAAACTC ACTACTGTGC AGATAGGCCA TGATAACTCT
    GGACTCTACG CCAAATGGTT GGTGGAATAT GTGATGGTTA GGAATGAGAT CACCGGACAT
    ACCTACAAGT TTCCATGTGG CCGCTGGTTG GGGAAGGGCA TGGATGACGG GAGCCTGGAG
    CGGGTTCTGG TCGGGGAGCT GCTCACATCC CAGCCTGAGG TGGATGAGAG GCCGTGCCGG
    ACTCCGCCCC TGCAGCAGTC TCCCAGCGTC ATCCGGAGGC TTGTTACCAT CTCACCCAAC
    AACAGACCCA AGCTGAACAC TGGGCAGATC CAAGAGTCCA TCGGTGAGGC AGTCAATGGC
    ATCGTGAAGC ACTTCCATAA GCCTGAAAAA GAGCGAGGTA GTCTGACGCT GTTGCTTTGT
    GGAGAATGTG GCCTTGTCTC GGCCCTGGAA CAGGCTTTCC AGCATGGATT TAAGTCACCA
    CGGCTCTTCA AAAATGTCTT CATTTGGGAT TTTTTGGAAA AAGCACAAAC CTATTATGAG
    ACTTTGGAGC AGAACGAACT TGTCCCTGAG GAAAACTGGC ATATGAGGGC CCGGAACTTC
    TGCCGATTTG TCACGGCAAT CAACAATAAT CCCCGGAACA TTGGCAAGGA TGGCAAGTTT
    CAGATGCTGG TGTGCTTGGG AGCCAGAGAC CACCTCCTGC ACCACTGGAT CGCCCTGCTG
    GCTGACTGCC CCATCACCGC ACACATGTAT GAGGATGTGG CACTGATCAA AGACCACACA
    CTTGTCAATT CCTTGATCCG CGTGCTGCAG ACACTGCAGG AGTTCAACAT CACGCTGGAG
    ACATCTCTGG TCAAGGGCAT CGATATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26409
    Clone ID Related Accession (Same CDS sequence) XM_002693106.4 , XM_001252217.6
    Accession Version XM_001252217.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DENN domain-containing protein 5A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001252217.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCGGCG GCGGCGGCGG CGGCGGAGGG GGCTCGGCGC CCAGTCGCTT CGCCGACTAC 
    TTTGTCATCT GCGGGCTGGA CACCGAGACC GGGCTGGAGC CGGACGAGCT GTCGGCATTA
    TGCCAGTACA TACAGGCTTC TAAAGCCAGG GATGGTGCCA GCTCTTTCAT TTCAAGTACA
    ACTGAAGGAG AAAATTTTGA GCAGACACCG TTGAGACGAA CATTCAAGTC TAAGGTCCTT
    GCACGATATC CTGAGAATGT GGACTGGAAT CCCTTTGACC AAGATGCAGT GGGAATGCTA
    TGCATGCCCA AAGGGCTGGC ATTCAAGACT CAGGCTGATC CCAGGGAGCC TCAGTTCCAT
    GCCTTTATTA TCACTAGGGA GGATGGCTCT CGGACCTTTG GGTTTGCTCT CACGTTTTAT
    GAGGAGGTGA CTAGCAAGCA GATCTGCAGT GCAATGCAGA CCCTCTACCA CATGCATAAT
    GCAGAGTATG ATGTCCTCCA TGCTCCACTT GCTGATAGCC GAGACCTCAG TGGCATGGAG
    GATGGTGATG GCACTCCTGG GACCAAGCTG CAGCGCTTCA ACTCCTACGA CATTAGCAGA
    GATACACTCT ACGTCTCTAA GTGCATCTGC CTCATCACGC CCATGTCCTT CATGAAAGCA
    TGTCGGAGTG TGCTGGAACA GCTTCACCAG GCAGTCACTT CGCCTCAGCC CCCTCCACTG
    CCCCTTGAGA GCTACATATA CAATGTACTA TATGAGGTGC CACTCCCACC TCCTGGCCGG
    TCCTTGAAGT TTTCTGGTGT CTATGGGCCA ATCGTCTGCC AGAGACCAAG TACCAATGAG
    CTTCCCCTGT TCGACTTTCC TGTCAAAGAG GTCTTTGAAC TGCTTGGGGT GGAGAATGTG
    TTTCAACTTT TTACTTGTGC CCTTCTGGAG TTTCAAATCC TGCTCTACTC ACAGCATTAC
    CAGCGACTGA TGACTGTGGC GGAGACAATT ACAGCTCTCA TGTTTCCTTT CCAGTGGCAG
    CATGTCTATG TTCCTATTCT CCCGGCTTCT CTCCTACATT TCTTAGATGC TCCTGTGCCA
    TACTTGATGG GCTTGCATTC CAATGGCTTG GATGACCGGT CAAAACTGGA GCTGCCTCAA
    GAGGCTAACC TCTGCTTTGT GGACATTGAC AACCACTTCA TTGAGTTACC AGAGGACTTG
    CCTCAGTTTC CCAACAAATT GGAGTTTGTC CAGGAAGTCT CTGAGATTCT CATGGCATTT
    GGCATTCCCC CTGAAGGAAG CCTTCACTGT AGCGAGAGTG CTTCCAAGCT GAAGAGGCTC
    CGGGCCTCTG AACTCGTCTC TGACAAGAGG AATGGAAACA TCGCAGGCTC CCCTTTACAT
    TCCTATGAGT TCCTCAAGGA GAATGAAACT ATCGCCCGCC TGCAGGCCTT GGTCAAGAGG
    ACTGGGGTGA GCCTGGAGAA GTTGGAAGTG CGTGAAGACC CCAGTAGCAA TAAGGATCTC
    AAAGTTCAGT GTGATGAAGA AGAACTCAGG ATTTACCAGC TAAACATTCA GATCCGGGAA
    GTTTTTGCAA ATCGTTTCAC TCAGATGTTT GCAGATTATG AGGTGTTTGT CATTCAGCCC
    AGCCAGGATA AGGAGTCCTG GTTTACCAAC AGAGAGCAGA TGCAAAATTT TGATAAAGCA
    TCTTTTCTGT CAGATCAGCC TGAACCCTAC CTGCCTTTCC TCTCAAGATT CTTGGAGACT
    CAGATGTTTG CTTCTTTCAT TGACAACAAA ATCATGTGTC ATGACGATGA TGATAAAGAT
    CCTGTGCTCC GGGTGTTTGA CTCCCGAGTA GACAAGATCA GGCTGTTGAA TGTTCGGACA
    CCTACTCTGC GCACATCCAT GTACCAGAAG TGTACCACAG TGGATGAAGC AAAGAAAGCA
    ATTGAGCTGC GATTGGCAAA AATTGATCAT ACTGCGGTTC ATCCCCATTT ACTTGACATG
    AAGATTGGAC AAGGGAAGTA TGAACCAGGC TTCTTCCCTA AGCTACAGTC TGATGTGCTT
    TCCACTGGGC CAGCCAGCAA CAAGTGGACA AAAAGAAATG CTCCTGCCCA GTGGAGGCGT
    AAAGACCGGC AGAAGCAGCA CACAGAACAC CTGCGCTTAG ACAATGACCA GAGAGAGAAG
    TACATCCAGG AAGCCAGGAA TATGGGCAGC ACCATCCGCC AGCCCAAACT GTCCAACCTC
    TCCCCGTCAG TGATTGCCCA GACCAACTGG AAGTTTGTGG AAGGTCTGCT GAAGGAATGC
    CGCAACAAGA CCAAGAGGAT GCTGGTGGAG AAGATGGGGC GAGAGGCTGT GGAACTAGGC
    CATGGGGAAG TGAACATCAC AGGGGTGGAA GAGAACACCT TGATTGCCAG TCTTTGTGAC
    CTACTGGAAA GGATTTGGAG TCATGGGCTA CAGGTGAAAC AGGGGAAATC AGCCTTGTGG
    TCCCACCTGT TACATTATCA GGAAAACCGG CAGAGAAAAC TCACATCAGG AAGCCTCAGT
    ACCTCAGGAA TATTTCTTGA TTCAGAGCGG AGGAAGTCTG ATGCCAGCTC CGTCATGTCT
    CCCCTGAAAA TCTCCCTCAT TCAGGATATG AGGCACATCC AGAACATTGG GGAAATCAAG
    ACTGATGTGG GAAAAGCCAG AGCGTGGGTG CGTCTGTCCA TGGAAAAAAA GTTACTTTCT
    AGACACCTGA AGCAGCTTCT CTCAGACCAT GAGCTCACCA AAAAACTCTA CAAGCGCTAC
    GCCTTCCTGC GCTGTGACGA CGAGAAGGAG CAGTTCCTCT ATCACCTCCT CTCTTTCAAT
    GCCGTCGATT ACTTCTGCTT CACCAATGTC TTCACGACTA TCCTGATTCC TTACCACATC
    CTGATTGTAC CAAGCAAGAA GCTGGGGGGC TCTATGTTCA CCGCCAACCC ATGGATCTGC
    ATCTCAGGAG AACTGGGTGA GACACAGATC CTGCAGATTC CCAGGAACCT GTTAGAGATG
    ACCTTCGAGT GCCAAAACTT GGGGAAACTC ACTACTGTGC AGATAGGCCA TGATAACTCT
    GGACTCTACG CCAAATGGTT GGTGGAATAT GTGATGGTTA GGAATGAGAT CACCGGACAT
    ACCTACAAGT TTCCATGTGG CCGCTGGTTG GGGAAGGGCA TGGATGACGG GAGCCTGGAG
    CGGGTTCTGG TCGGGGAGCT GCTCACATCC CAGCCTGAGG TGGATGAGAG GCCGTGCCGG
    ACTCCGCCCC TGCAGCAGTC TCCCAGCGTC ATCCGGAGGC TTGTTACCAT CTCACCCAAC
    AACAGACCCA AGCTGAACAC TGGGCAGATC CAAGAGTCCA TCGGTGAGGC AGTCAATGGC
    ATCGTGAAGC ACTTCCATAA GCCTGAAAAA GAGCGAGGTA GTCTGACGCT GTTGCTTTGT
    GGAGAATGTG GCCTTGTCTC GGCCCTGGAA CAGGCTTTCC AGCATGGATT TAAGTCACCA
    CGGCTCTTCA AAAATGTCTT CATTTGGGAT TTTTTGGAAA AAGCACAAAC CTATTATGAG
    ACTTTGGAGC AGAACGAACT TGTCCCTGAG GAAAACTGGC ATATGAGGGC CCGGAACTTC
    TGCCGATTTG TCACGGCAAT CAACAATAAT CCCCGGAACA TTGGCAAGGA TGGCAAGTTT
    CAGATGCTGG TGTGCTTGGG AGCCAGAGAC CACCTCCTGC ACCACTGGAT CGCCCTGCTG
    GCTGACTGCC CCATCACCGC ACACATGTAT GAGGATGTGG CACTGATCAA AGACCACACA
    CTTGTCAATT CCTTGATCCG CGTGCTGCAG ACACTGCAGG AGTTCAACAT CACGCTGGAG
    ACATCTCTGG TCAAGGGCAT CGATATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001252218.2
    CDS68..3937
    Translation

    Target ORF information:

    RefSeq Version XM_001252217.6
    Organism Bos taurus(cattle)
    Definition Bos taurus DENN/MADD domain containing 5A (DENND5A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001252217.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCGGCG GCGGCGGCGG CGGCGGAGGG GGCTCGGCGC CCAGTCGCTT CGCCGACTAC 
    TTTGTCATCT GCGGGCTGGA CACCGAGACC GGGCTGGAGC CGGACGAGCT GTCGGCATTA
    TGCCAGTACA TACAGGCTTC TAAAGCCAGG GATGGTGCCA GCTCTTTCAT TTCAAGTACA
    ACTGAAGGAG AAAATTTTGA GCAGACACCG TTGAGACGAA CATTCAAGTC TAAGGTCCTT
    GCACGATATC CTGAGAATGT GGACTGGAAT CCCTTTGACC AAGATGCAGT GGGAATGCTA
    TGCATGCCCA AAGGGCTGGC ATTCAAGACT CAGGCTGATC CCAGGGAGCC TCAGTTCCAT
    GCCTTTATTA TCACTAGGGA GGATGGCTCT CGGACCTTTG GGTTTGCTCT CACGTTTTAT
    GAGGAGGTGA CTAGCAAGCA GATCTGCAGT GCAATGCAGA CCCTCTACCA CATGCATAAT
    GCAGAGTATG ATGTCCTCCA TGCTCCACTT GCTGATAGCC GAGACCTCAG TGGCATGGAG
    GATGGTGATG GCACTCCTGG GACCAAGCTG CAGCGCTTCA ACTCCTACGA CATTAGCAGA
    GATACACTCT ACGTCTCTAA GTGCATCTGC CTCATCACGC CCATGTCCTT CATGAAAGCA
    TGTCGGAGTG TGCTGGAACA GCTTCACCAG GCAGTCACTT CGCCTCAGCC CCCTCCACTG
    CCCCTTGAGA GCTACATATA CAATGTACTA TATGAGGTGC CACTCCCACC TCCTGGCCGG
    TCCTTGAAGT TTTCTGGTGT CTATGGGCCA ATCGTCTGCC AGAGACCAAG TACCAATGAG
    CTTCCCCTGT TCGACTTTCC TGTCAAAGAG GTCTTTGAAC TGCTTGGGGT GGAGAATGTG
    TTTCAACTTT TTACTTGTGC CCTTCTGGAG TTTCAAATCC TGCTCTACTC ACAGCATTAC
    CAGCGACTGA TGACTGTGGC GGAGACAATT ACAGCTCTCA TGTTTCCTTT CCAGTGGCAG
    CATGTCTATG TTCCTATTCT CCCGGCTTCT CTCCTACATT TCTTAGATGC TCCTGTGCCA
    TACTTGATGG GCTTGCATTC CAATGGCTTG GATGACCGGT CAAAACTGGA GCTGCCTCAA
    GAGGCTAACC TCTGCTTTGT GGACATTGAC AACCACTTCA TTGAGTTACC AGAGGACTTG
    CCTCAGTTTC CCAACAAATT GGAGTTTGTC CAGGAAGTCT CTGAGATTCT CATGGCATTT
    GGCATTCCCC CTGAAGGAAG CCTTCACTGT AGCGAGAGTG CTTCCAAGCT GAAGAGGCTC
    CGGGCCTCTG AACTCGTCTC TGACAAGAGG AATGGAAACA TCGCAGGCTC CCCTTTACAT
    TCCTATGAGT TCCTCAAGGA GAATGAAACT ATCGCCCGCC TGCAGGCCTT GGTCAAGAGG
    ACTGGGGTGA GCCTGGAGAA GTTGGAAGTG CGTGAAGACC CCAGTAGCAA TAAGGATCTC
    AAAGTTCAGT GTGATGAAGA AGAACTCAGG ATTTACCAGC TAAACATTCA GATCCGGGAA
    GTTTTTGCAA ATCGTTTCAC TCAGATGTTT GCAGATTATG AGGTGTTTGT CATTCAGCCC
    AGCCAGGATA AGGAGTCCTG GTTTACCAAC AGAGAGCAGA TGCAAAATTT TGATAAAGCA
    TCTTTTCTGT CAGATCAGCC TGAACCCTAC CTGCCTTTCC TCTCAAGATT CTTGGAGACT
    CAGATGTTTG CTTCTTTCAT TGACAACAAA ATCATGTGTC ATGACGATGA TGATAAAGAT
    CCTGTGCTCC GGGTGTTTGA CTCCCGAGTA GACAAGATCA GGCTGTTGAA TGTTCGGACA
    CCTACTCTGC GCACATCCAT GTACCAGAAG TGTACCACAG TGGATGAAGC AAAGAAAGCA
    ATTGAGCTGC GATTGGCAAA AATTGATCAT ACTGCGGTTC ATCCCCATTT ACTTGACATG
    AAGATTGGAC AAGGGAAGTA TGAACCAGGC TTCTTCCCTA AGCTACAGTC TGATGTGCTT
    TCCACTGGGC CAGCCAGCAA CAAGTGGACA AAAAGAAATG CTCCTGCCCA GTGGAGGCGT
    AAAGACCGGC AGAAGCAGCA CACAGAACAC CTGCGCTTAG ACAATGACCA GAGAGAGAAG
    TACATCCAGG AAGCCAGGAA TATGGGCAGC ACCATCCGCC AGCCCAAACT GTCCAACCTC
    TCCCCGTCAG TGATTGCCCA GACCAACTGG AAGTTTGTGG AAGGTCTGCT GAAGGAATGC
    CGCAACAAGA CCAAGAGGAT GCTGGTGGAG AAGATGGGGC GAGAGGCTGT GGAACTAGGC
    CATGGGGAAG TGAACATCAC AGGGGTGGAA GAGAACACCT TGATTGCCAG TCTTTGTGAC
    CTACTGGAAA GGATTTGGAG TCATGGGCTA CAGGTGAAAC AGGGGAAATC AGCCTTGTGG
    TCCCACCTGT TACATTATCA GGAAAACCGG CAGAGAAAAC TCACATCAGG AAGCCTCAGT
    ACCTCAGGAA TATTTCTTGA TTCAGAGCGG AGGAAGTCTG ATGCCAGCTC CGTCATGTCT
    CCCCTGAAAA TCTCCCTCAT TCAGGATATG AGGCACATCC AGAACATTGG GGAAATCAAG
    ACTGATGTGG GAAAAGCCAG AGCGTGGGTG CGTCTGTCCA TGGAAAAAAA GTTACTTTCT
    AGACACCTGA AGCAGCTTCT CTCAGACCAT GAGCTCACCA AAAAACTCTA CAAGCGCTAC
    GCCTTCCTGC GCTGTGACGA CGAGAAGGAG CAGTTCCTCT ATCACCTCCT CTCTTTCAAT
    GCCGTCGATT ACTTCTGCTT CACCAATGTC TTCACGACTA TCCTGATTCC TTACCACATC
    CTGATTGTAC CAAGCAAGAA GCTGGGGGGC TCTATGTTCA CCGCCAACCC ATGGATCTGC
    ATCTCAGGAG AACTGGGTGA GACACAGATC CTGCAGATTC CCAGGAACCT GTTAGAGATG
    ACCTTCGAGT GCCAAAACTT GGGGAAACTC ACTACTGTGC AGATAGGCCA TGATAACTCT
    GGACTCTACG CCAAATGGTT GGTGGAATAT GTGATGGTTA GGAATGAGAT CACCGGACAT
    ACCTACAAGT TTCCATGTGG CCGCTGGTTG GGGAAGGGCA TGGATGACGG GAGCCTGGAG
    CGGGTTCTGG TCGGGGAGCT GCTCACATCC CAGCCTGAGG TGGATGAGAG GCCGTGCCGG
    ACTCCGCCCC TGCAGCAGTC TCCCAGCGTC ATCCGGAGGC TTGTTACCAT CTCACCCAAC
    AACAGACCCA AGCTGAACAC TGGGCAGATC CAAGAGTCCA TCGGTGAGGC AGTCAATGGC
    ATCGTGAAGC ACTTCCATAA GCCTGAAAAA GAGCGAGGTA GTCTGACGCT GTTGCTTTGT
    GGAGAATGTG GCCTTGTCTC GGCCCTGGAA CAGGCTTTCC AGCATGGATT TAAGTCACCA
    CGGCTCTTCA AAAATGTCTT CATTTGGGAT TTTTTGGAAA AAGCACAAAC CTATTATGAG
    ACTTTGGAGC AGAACGAACT TGTCCCTGAG GAAAACTGGC ATATGAGGGC CCGGAACTTC
    TGCCGATTTG TCACGGCAAT CAACAATAAT CCCCGGAACA TTGGCAAGGA TGGCAAGTTT
    CAGATGCTGG TGTGCTTGGG AGCCAGAGAC CACCTCCTGC ACCACTGGAT CGCCCTGCTG
    GCTGACTGCC CCATCACCGC ACACATGTAT GAGGATGTGG CACTGATCAA AGACCACACA
    CTTGTCAATT CCTTGATCCG CGTGCTGCAG ACACTGCAGG AGTTCAACAT CACGCTGGAG
    ACATCTCTGG TCAAGGGCAT CGATATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26411
    Clone ID Related Accession (Same CDS sequence) XM_005194609.3 , XM_005216096.3
    Accession Version XM_005194609.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DENN domain-containing protein 5A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005194609.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGCGGCG GCGGCGGCGG CGGCGGAGGG GGCTCGGCGC CCAGTCGCTT CGCCGACTAC 
    TTTGTCATCT GCGGGCTGGA CACCGAGACC GGGCTGGAGC CGGACGAGCT GTCGGCATTA
    TGCCAGTACA TACAGGCTTC TAAAGCCAGG GATGGTGCCA GCTCTTTCAT TTCAAGTACA
    ACTGAAGGAG AAAATTTTGA GCAGACACCG TTGAGACGAA CATTCAAGTC TAAGGTCCTT
    GCACGATATC CTGAGAATGT GGACTGGAAT CCCTTTGACC AAGATGCAGT GGGAATGCTA
    TGCATGCCCA AAGGGCTGGC ATTCAAGACT CAGGCTGATC CCAGGGAGCC TCAGTTCCAT
    GCCTTTATTA TCACTAGGGA GGATGGCTCT CGGACCTTTG GGTTTGCTCT CACGTTTTAT
    GAGGAGGTGA CTAGCAAGCA GATCTGCAGT GCAATGCAGA CCCTCTACCA CATGCATAAT
    GCAGAGTATG ATGTCCTCCA TGCTCCACTT GCTGATAGCC GAGACCTCAG TGGCATGGAG
    GATGGTGATG GCACTCCTGG GACCAAGCTG CAGCGCTTCA ACTCCTACGA CATTAGCAGA
    GATACACTCT ACGTCTCTAA GTGCATCTGC CTCATCACGC CCATGTCCTT CATGAAAGCA
    TGTCGGAGTG TGCTGGAACA GCTTCACCAG GCAGTCACTT CGCCTCAGCC CCCTCCACTG
    CCCCTTGAGA GCTACATATA CAATGTACTA TATGAGGTGC CACTCCCACC TCCTGGCCGG
    TCCTTGAAGT TTTCTGGTGT CTATGGGCCA ATCGTCTGCC AGAGACCAAG TACCAATGAG
    CTTCCCCTGT TCGACTTTCC TGTCAAAGAG GTCTTTGAAC TGCTTGGGGT GGAGAATGTG
    TTTCAACTTT TTACTTGTGC CCTTCTGGAG TTTCAAATCC TGCTCTACTC ACAGCATTAC
    CAGCGACTGA TGACTGTGGC GGAGACAATT ACAGCTCTCA TGTTTCCTTT CCAGTGGCAG
    CATGTCTATG TTCCTATTCT CCCGGCTTCT CTCCTACATT TCTTAGATGC TCCTGTGCCA
    TACTTGATGG GCTTGCATTC CAATGGCTTG GATGACCGGT CAAAACTGGA GCTGCCTCAA
    GAGGCTAACC TCTGCTTTGT GGACATTGAC AACCACTTCA TTGAGTTACC AGAGGACTTG
    CCTCAGTTTC CCAACAAATT GGAGTTTGTC CAGGAAGTCT CTGAGATTCT CATGGCATTT
    GGCATTCCCC CTGAAGGAAG CCTTCACTGT AGCGAGAGTG CTTCCAAGCT GAAGAGGCTC
    CGGGCCTCTG AACTCGTCTC TGACAAGAGG AATGGAAACA TCGCAGGCTC CCCTTTACAT
    TCCTATGAGT TCCTCAAGGA GAATGAAACT ATCGCCCGCC TGCAGGCCTT GGTCAAGAGG
    ACTGGGGTGA GCCTGGAGAA GTTGGAAGTG CGTGAAGACC CCAGTAGCAA TAAGGATCTC
    AAAGTTCAGT GTGATGAAGA AGAACTCAGG ATTTACCAGC TAAACATTCA GATCCGGGAA
    GTTTTTGCAA ATCGTTTCAC TCAGATGTTT GCAGATTATG AGGTGTTTGT CATTCAGCCC
    AGCCAGGATA AGGAGTCCTG GTTTACCAAC AGAGAGCAGA TGCAAAATTT TGATAAAGCA
    TCTTTTCTGT CAGATCAGCC TGAACCCTAC CTGCCTTTCC TCTCAAGATT CTTGGAGACT
    CAGATGTTTG CTTCTTTCAT TGACAACAAA ATCATGTGTC ATGACGATGA TGATAAAGAT
    CCTGTGCTCC GGGTGTTTGA CTCCCGAGTA GACAAGATCA GGCTGTTGAA TGTTCGGACA
    CCTACTCTGC GCACATCCAT GTACCAGAAG TGTACCACAG TGGATGAAGC AAAGAAAGCA
    ATTGAGCTGC GATTGGCAAA AATTGATCAT ACTGCGGTTC ATCCCCATTT ACTTGACATG
    AAGATTGGAC AAGGGAAGTA TGAACCAGGC TTCTTCCCTA AGCTACAGTC TGATGTGCTT
    TCCACTGGGC CAGCCAGCAA CAAGTGGACA AAAAGAAATG CTCCTGCCCA GTGGAGGCGT
    AAAGACCGGC AGAAGCAGCA CACAGAACAC CTGCGCTTAG ACAATGACCA GAGAGAGAAG
    TACATCCAGG AAGCCAGGAA TATGGGCAGC ACCATCCGCC AGCCCAAACT GTCCAACCTC
    TCCCCGTCAG TGATTGCCCA GACCAACTGG AAGTTTGTGG AAGGTCTGCT GAAGGAATGC
    CGCAACAAGA CCAAGAGGAT GCTGGTGGAG AAGATGGGGC GAGAGGCTGT GGAACTAGGC
    CATGGGGAAG TGAACATCAC AGGGGTGGAA GAGAACACCT TGATTGCCAG TCTTTGTGAC
    CTACTGGAAA GGATTTGGAG TCATGGGCTA CAGGTGAAAC AGGGGAAATC AGCCTTGTGG
    TCCCACCTGT TACATTATCA GGAAAACCGG CAGAGAAAAC TCACATCAGG AAGCCTCAGT
    ACCTCAGGAA TATTTCTTGA TTCAGAGCGG AGGAAGTCTG ATGCCAGCTC CGTCATGTCT
    CCCCTGAAAA TCTCCCTCAT TCAGGATATG AGGCACATCC AGAACATTGG GGAAATCAAG
    ACTGATGTGG GAAAAGCCAG AGCGTGGGTG CGTCTGTCCA TGGAAAAAAA GTTACTTTCT
    AGACACCTGA AGCAGCTTCT CTCAGACCAT GAGCTCACCA AAAAACTCTA CAAGCGCTAC
    GCCTTCCTGC GCTGTGACGA CGAGAAGGAG CAGTTCCTCT ATCACCTCCT CTCTTTCAAT
    GCCGTCGATT ACTTCTGCTT CACCAATGTC TTCACGACTA TCCTGATTCC TTACCACATC
    CTGATTGTAC CAAGCAAGAA GCTGGGGGGC TCTATGTTCA CCGCCAACCC ATGGATCTGC
    ATCTCAGGAG AACTGGGTGA GACACAGATC CTGCAGATTC CCAGGAACCT GTTAGAGATG
    ACCTTCGAGT GCCAAAACTT GGGGAAACTC ACTACTGTGC AGATAGGCCA TGATAACTCT
    GGACTCTACG CCAAATGGTT GGTGGAATAT GTGATGGTTA GGAATGAGAT CACCGGACAT
    ACCTACAAGT TTCCATGTGG CCGCTGGTTG GGGAAGGGCA TGGATGACGG GAGCCTGGAG
    CGGGTTCTGG TCGGGGAGCT GCTCACATCC CAGCCTGAGG TGGATGAGAG GCCGTGCCGG
    ACTCCGCCCC TGCAGCAGTC TCCCAGCGTC ATCCGGAGGC TTGTTACCAT CTCACCCAAC
    AACAGACCCA AGCTGAACAC TGGGCAGATC CAAGAGTCCA TCGGTGAGGC AGTCAATGGC
    ATCGTGAAGC ACTTCCATAA GCCTGAAAAA GAGCGAGGTA GTCTGACGCT GTTGCTTTGT
    GGAGAATGTG GCCTTGTCTC GGCCCTGGAA CAGGCTTTCC AGCATGGATT TAAGTCACCA
    CGGCTCTTCA AAAATGTCTT CATTTGGGAT TTTTTGGAAA AAGCACAAAC CTATTATGAG
    ACTTTGGAGC AGAACGAACT TGTCCCTGAG GAAAACTGGC ATATGAGGGC CCGGAACTTC
    TGCCGATTTG TCACGGCAAT CAACAATAAT CCCCGGAACA TTGGCAAGGA TGAGACCACC
    TCCTGCACCA CTGGATCGCC CTGCTGGCTG ACTGCCCCAT CACCGCACAC ATGTATGAGG
    ATGTGGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005194666.2
    CDS22..3753
    Translation

    Target ORF information:

    RefSeq Version XM_005194609.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DENN/MADD domain containing 5A (DENND5A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005194609.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGCGGCG GCGGCGGCGG CGGCGGAGGG GGCTCGGCGC CCAGTCGCTT CGCCGACTAC 
    TTTGTCATCT GCGGGCTGGA CACCGAGACC GGGCTGGAGC CGGACGAGCT GTCGGCATTA
    TGCCAGTACA TACAGGCTTC TAAAGCCAGG GATGGTGCCA GCTCTTTCAT TTCAAGTACA
    ACTGAAGGAG AAAATTTTGA GCAGACACCG TTGAGACGAA CATTCAAGTC TAAGGTCCTT
    GCACGATATC CTGAGAATGT GGACTGGAAT CCCTTTGACC AAGATGCAGT GGGAATGCTA
    TGCATGCCCA AAGGGCTGGC ATTCAAGACT CAGGCTGATC CCAGGGAGCC TCAGTTCCAT
    GCCTTTATTA TCACTAGGGA GGATGGCTCT CGGACCTTTG GGTTTGCTCT CACGTTTTAT
    GAGGAGGTGA CTAGCAAGCA GATCTGCAGT GCAATGCAGA CCCTCTACCA CATGCATAAT
    GCAGAGTATG ATGTCCTCCA TGCTCCACTT GCTGATAGCC GAGACCTCAG TGGCATGGAG
    GATGGTGATG GCACTCCTGG GACCAAGCTG CAGCGCTTCA ACTCCTACGA CATTAGCAGA
    GATACACTCT ACGTCTCTAA GTGCATCTGC CTCATCACGC CCATGTCCTT CATGAAAGCA
    TGTCGGAGTG TGCTGGAACA GCTTCACCAG GCAGTCACTT CGCCTCAGCC CCCTCCACTG
    CCCCTTGAGA GCTACATATA CAATGTACTA TATGAGGTGC CACTCCCACC TCCTGGCCGG
    TCCTTGAAGT TTTCTGGTGT CTATGGGCCA ATCGTCTGCC AGAGACCAAG TACCAATGAG
    CTTCCCCTGT TCGACTTTCC TGTCAAAGAG GTCTTTGAAC TGCTTGGGGT GGAGAATGTG
    TTTCAACTTT TTACTTGTGC CCTTCTGGAG TTTCAAATCC TGCTCTACTC ACAGCATTAC
    CAGCGACTGA TGACTGTGGC GGAGACAATT ACAGCTCTCA TGTTTCCTTT CCAGTGGCAG
    CATGTCTATG TTCCTATTCT CCCGGCTTCT CTCCTACATT TCTTAGATGC TCCTGTGCCA
    TACTTGATGG GCTTGCATTC CAATGGCTTG GATGACCGGT CAAAACTGGA GCTGCCTCAA
    GAGGCTAACC TCTGCTTTGT GGACATTGAC AACCACTTCA TTGAGTTACC AGAGGACTTG
    CCTCAGTTTC CCAACAAATT GGAGTTTGTC CAGGAAGTCT CTGAGATTCT CATGGCATTT
    GGCATTCCCC CTGAAGGAAG CCTTCACTGT AGCGAGAGTG CTTCCAAGCT GAAGAGGCTC
    CGGGCCTCTG AACTCGTCTC TGACAAGAGG AATGGAAACA TCGCAGGCTC CCCTTTACAT
    TCCTATGAGT TCCTCAAGGA GAATGAAACT ATCGCCCGCC TGCAGGCCTT GGTCAAGAGG
    ACTGGGGTGA GCCTGGAGAA GTTGGAAGTG CGTGAAGACC CCAGTAGCAA TAAGGATCTC
    AAAGTTCAGT GTGATGAAGA AGAACTCAGG ATTTACCAGC TAAACATTCA GATCCGGGAA
    GTTTTTGCAA ATCGTTTCAC TCAGATGTTT GCAGATTATG AGGTGTTTGT CATTCAGCCC
    AGCCAGGATA AGGAGTCCTG GTTTACCAAC AGAGAGCAGA TGCAAAATTT TGATAAAGCA
    TCTTTTCTGT CAGATCAGCC TGAACCCTAC CTGCCTTTCC TCTCAAGATT CTTGGAGACT
    CAGATGTTTG CTTCTTTCAT TGACAACAAA ATCATGTGTC ATGACGATGA TGATAAAGAT
    CCTGTGCTCC GGGTGTTTGA CTCCCGAGTA GACAAGATCA GGCTGTTGAA TGTTCGGACA
    CCTACTCTGC GCACATCCAT GTACCAGAAG TGTACCACAG TGGATGAAGC AAAGAAAGCA
    ATTGAGCTGC GATTGGCAAA AATTGATCAT ACTGCGGTTC ATCCCCATTT ACTTGACATG
    AAGATTGGAC AAGGGAAGTA TGAACCAGGC TTCTTCCCTA AGCTACAGTC TGATGTGCTT
    TCCACTGGGC CAGCCAGCAA CAAGTGGACA AAAAGAAATG CTCCTGCCCA GTGGAGGCGT
    AAAGACCGGC AGAAGCAGCA CACAGAACAC CTGCGCTTAG ACAATGACCA GAGAGAGAAG
    TACATCCAGG AAGCCAGGAA TATGGGCAGC ACCATCCGCC AGCCCAAACT GTCCAACCTC
    TCCCCGTCAG TGATTGCCCA GACCAACTGG AAGTTTGTGG AAGGTCTGCT GAAGGAATGC
    CGCAACAAGA CCAAGAGGAT GCTGGTGGAG AAGATGGGGC GAGAGGCTGT GGAACTAGGC
    CATGGGGAAG TGAACATCAC AGGGGTGGAA GAGAACACCT TGATTGCCAG TCTTTGTGAC
    CTACTGGAAA GGATTTGGAG TCATGGGCTA CAGGTGAAAC AGGGGAAATC AGCCTTGTGG
    TCCCACCTGT TACATTATCA GGAAAACCGG CAGAGAAAAC TCACATCAGG AAGCCTCAGT
    ACCTCAGGAA TATTTCTTGA TTCAGAGCGG AGGAAGTCTG ATGCCAGCTC CGTCATGTCT
    CCCCTGAAAA TCTCCCTCAT TCAGGATATG AGGCACATCC AGAACATTGG GGAAATCAAG
    ACTGATGTGG GAAAAGCCAG AGCGTGGGTG CGTCTGTCCA TGGAAAAAAA GTTACTTTCT
    AGACACCTGA AGCAGCTTCT CTCAGACCAT GAGCTCACCA AAAAACTCTA CAAGCGCTAC
    GCCTTCCTGC GCTGTGACGA CGAGAAGGAG CAGTTCCTCT ATCACCTCCT CTCTTTCAAT
    GCCGTCGATT ACTTCTGCTT CACCAATGTC TTCACGACTA TCCTGATTCC TTACCACATC
    CTGATTGTAC CAAGCAAGAA GCTGGGGGGC TCTATGTTCA CCGCCAACCC ATGGATCTGC
    ATCTCAGGAG AACTGGGTGA GACACAGATC CTGCAGATTC CCAGGAACCT GTTAGAGATG
    ACCTTCGAGT GCCAAAACTT GGGGAAACTC ACTACTGTGC AGATAGGCCA TGATAACTCT
    GGACTCTACG CCAAATGGTT GGTGGAATAT GTGATGGTTA GGAATGAGAT CACCGGACAT
    ACCTACAAGT TTCCATGTGG CCGCTGGTTG GGGAAGGGCA TGGATGACGG GAGCCTGGAG
    CGGGTTCTGG TCGGGGAGCT GCTCACATCC CAGCCTGAGG TGGATGAGAG GCCGTGCCGG
    ACTCCGCCCC TGCAGCAGTC TCCCAGCGTC ATCCGGAGGC TTGTTACCAT CTCACCCAAC
    AACAGACCCA AGCTGAACAC TGGGCAGATC CAAGAGTCCA TCGGTGAGGC AGTCAATGGC
    ATCGTGAAGC ACTTCCATAA GCCTGAAAAA GAGCGAGGTA GTCTGACGCT GTTGCTTTGT
    GGAGAATGTG GCCTTGTCTC GGCCCTGGAA CAGGCTTTCC AGCATGGATT TAAGTCACCA
    CGGCTCTTCA AAAATGTCTT CATTTGGGAT TTTTTGGAAA AAGCACAAAC CTATTATGAG
    ACTTTGGAGC AGAACGAACT TGTCCCTGAG GAAAACTGGC ATATGAGGGC CCGGAACTTC
    TGCCGATTTG TCACGGCAAT CAACAATAAT CCCCGGAACA TTGGCAAGGA TGAGACCACC
    TCCTGCACCA CTGGATCGCC CTGCTGGCTG ACTGCCCCAT CACCGCACAC ATGTATGAGG
    ATGTGGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26411
    Clone ID Related Accession (Same CDS sequence) XM_005194609.3 , XM_005216096.3
    Accession Version XM_005216096.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DENN domain-containing protein 5A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216096.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGCGGCG GCGGCGGCGG CGGCGGAGGG GGCTCGGCGC CCAGTCGCTT CGCCGACTAC 
    TTTGTCATCT GCGGGCTGGA CACCGAGACC GGGCTGGAGC CGGACGAGCT GTCGGCATTA
    TGCCAGTACA TACAGGCTTC TAAAGCCAGG GATGGTGCCA GCTCTTTCAT TTCAAGTACA
    ACTGAAGGAG AAAATTTTGA GCAGACACCG TTGAGACGAA CATTCAAGTC TAAGGTCCTT
    GCACGATATC CTGAGAATGT GGACTGGAAT CCCTTTGACC AAGATGCAGT GGGAATGCTA
    TGCATGCCCA AAGGGCTGGC ATTCAAGACT CAGGCTGATC CCAGGGAGCC TCAGTTCCAT
    GCCTTTATTA TCACTAGGGA GGATGGCTCT CGGACCTTTG GGTTTGCTCT CACGTTTTAT
    GAGGAGGTGA CTAGCAAGCA GATCTGCAGT GCAATGCAGA CCCTCTACCA CATGCATAAT
    GCAGAGTATG ATGTCCTCCA TGCTCCACTT GCTGATAGCC GAGACCTCAG TGGCATGGAG
    GATGGTGATG GCACTCCTGG GACCAAGCTG CAGCGCTTCA ACTCCTACGA CATTAGCAGA
    GATACACTCT ACGTCTCTAA GTGCATCTGC CTCATCACGC CCATGTCCTT CATGAAAGCA
    TGTCGGAGTG TGCTGGAACA GCTTCACCAG GCAGTCACTT CGCCTCAGCC CCCTCCACTG
    CCCCTTGAGA GCTACATATA CAATGTACTA TATGAGGTGC CACTCCCACC TCCTGGCCGG
    TCCTTGAAGT TTTCTGGTGT CTATGGGCCA ATCGTCTGCC AGAGACCAAG TACCAATGAG
    CTTCCCCTGT TCGACTTTCC TGTCAAAGAG GTCTTTGAAC TGCTTGGGGT GGAGAATGTG
    TTTCAACTTT TTACTTGTGC CCTTCTGGAG TTTCAAATCC TGCTCTACTC ACAGCATTAC
    CAGCGACTGA TGACTGTGGC GGAGACAATT ACAGCTCTCA TGTTTCCTTT CCAGTGGCAG
    CATGTCTATG TTCCTATTCT CCCGGCTTCT CTCCTACATT TCTTAGATGC TCCTGTGCCA
    TACTTGATGG GCTTGCATTC CAATGGCTTG GATGACCGGT CAAAACTGGA GCTGCCTCAA
    GAGGCTAACC TCTGCTTTGT GGACATTGAC AACCACTTCA TTGAGTTACC AGAGGACTTG
    CCTCAGTTTC CCAACAAATT GGAGTTTGTC CAGGAAGTCT CTGAGATTCT CATGGCATTT
    GGCATTCCCC CTGAAGGAAG CCTTCACTGT AGCGAGAGTG CTTCCAAGCT GAAGAGGCTC
    CGGGCCTCTG AACTCGTCTC TGACAAGAGG AATGGAAACA TCGCAGGCTC CCCTTTACAT
    TCCTATGAGT TCCTCAAGGA GAATGAAACT ATCGCCCGCC TGCAGGCCTT GGTCAAGAGG
    ACTGGGGTGA GCCTGGAGAA GTTGGAAGTG CGTGAAGACC CCAGTAGCAA TAAGGATCTC
    AAAGTTCAGT GTGATGAAGA AGAACTCAGG ATTTACCAGC TAAACATTCA GATCCGGGAA
    GTTTTTGCAA ATCGTTTCAC TCAGATGTTT GCAGATTATG AGGTGTTTGT CATTCAGCCC
    AGCCAGGATA AGGAGTCCTG GTTTACCAAC AGAGAGCAGA TGCAAAATTT TGATAAAGCA
    TCTTTTCTGT CAGATCAGCC TGAACCCTAC CTGCCTTTCC TCTCAAGATT CTTGGAGACT
    CAGATGTTTG CTTCTTTCAT TGACAACAAA ATCATGTGTC ATGACGATGA TGATAAAGAT
    CCTGTGCTCC GGGTGTTTGA CTCCCGAGTA GACAAGATCA GGCTGTTGAA TGTTCGGACA
    CCTACTCTGC GCACATCCAT GTACCAGAAG TGTACCACAG TGGATGAAGC AAAGAAAGCA
    ATTGAGCTGC GATTGGCAAA AATTGATCAT ACTGCGGTTC ATCCCCATTT ACTTGACATG
    AAGATTGGAC AAGGGAAGTA TGAACCAGGC TTCTTCCCTA AGCTACAGTC TGATGTGCTT
    TCCACTGGGC CAGCCAGCAA CAAGTGGACA AAAAGAAATG CTCCTGCCCA GTGGAGGCGT
    AAAGACCGGC AGAAGCAGCA CACAGAACAC CTGCGCTTAG ACAATGACCA GAGAGAGAAG
    TACATCCAGG AAGCCAGGAA TATGGGCAGC ACCATCCGCC AGCCCAAACT GTCCAACCTC
    TCCCCGTCAG TGATTGCCCA GACCAACTGG AAGTTTGTGG AAGGTCTGCT GAAGGAATGC
    CGCAACAAGA CCAAGAGGAT GCTGGTGGAG AAGATGGGGC GAGAGGCTGT GGAACTAGGC
    CATGGGGAAG TGAACATCAC AGGGGTGGAA GAGAACACCT TGATTGCCAG TCTTTGTGAC
    CTACTGGAAA GGATTTGGAG TCATGGGCTA CAGGTGAAAC AGGGGAAATC AGCCTTGTGG
    TCCCACCTGT TACATTATCA GGAAAACCGG CAGAGAAAAC TCACATCAGG AAGCCTCAGT
    ACCTCAGGAA TATTTCTTGA TTCAGAGCGG AGGAAGTCTG ATGCCAGCTC CGTCATGTCT
    CCCCTGAAAA TCTCCCTCAT TCAGGATATG AGGCACATCC AGAACATTGG GGAAATCAAG
    ACTGATGTGG GAAAAGCCAG AGCGTGGGTG CGTCTGTCCA TGGAAAAAAA GTTACTTTCT
    AGACACCTGA AGCAGCTTCT CTCAGACCAT GAGCTCACCA AAAAACTCTA CAAGCGCTAC
    GCCTTCCTGC GCTGTGACGA CGAGAAGGAG CAGTTCCTCT ATCACCTCCT CTCTTTCAAT
    GCCGTCGATT ACTTCTGCTT CACCAATGTC TTCACGACTA TCCTGATTCC TTACCACATC
    CTGATTGTAC CAAGCAAGAA GCTGGGGGGC TCTATGTTCA CCGCCAACCC ATGGATCTGC
    ATCTCAGGAG AACTGGGTGA GACACAGATC CTGCAGATTC CCAGGAACCT GTTAGAGATG
    ACCTTCGAGT GCCAAAACTT GGGGAAACTC ACTACTGTGC AGATAGGCCA TGATAACTCT
    GGACTCTACG CCAAATGGTT GGTGGAATAT GTGATGGTTA GGAATGAGAT CACCGGACAT
    ACCTACAAGT TTCCATGTGG CCGCTGGTTG GGGAAGGGCA TGGATGACGG GAGCCTGGAG
    CGGGTTCTGG TCGGGGAGCT GCTCACATCC CAGCCTGAGG TGGATGAGAG GCCGTGCCGG
    ACTCCGCCCC TGCAGCAGTC TCCCAGCGTC ATCCGGAGGC TTGTTACCAT CTCACCCAAC
    AACAGACCCA AGCTGAACAC TGGGCAGATC CAAGAGTCCA TCGGTGAGGC AGTCAATGGC
    ATCGTGAAGC ACTTCCATAA GCCTGAAAAA GAGCGAGGTA GTCTGACGCT GTTGCTTTGT
    GGAGAATGTG GCCTTGTCTC GGCCCTGGAA CAGGCTTTCC AGCATGGATT TAAGTCACCA
    CGGCTCTTCA AAAATGTCTT CATTTGGGAT TTTTTGGAAA AAGCACAAAC CTATTATGAG
    ACTTTGGAGC AGAACGAACT TGTCCCTGAG GAAAACTGGC ATATGAGGGC CCGGAACTTC
    TGCCGATTTG TCACGGCAAT CAACAATAAT CCCCGGAACA TTGGCAAGGA TGAGACCACC
    TCCTGCACCA CTGGATCGCC CTGCTGGCTG ACTGCCCCAT CACCGCACAC ATGTATGAGG
    ATGTGGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216153.1
    CDS22..3753
    Translation

    Target ORF information:

    RefSeq Version XM_005216096.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DENN/MADD domain containing 5A (DENND5A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216096.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGCGGCG GCGGCGGCGG CGGCGGAGGG GGCTCGGCGC CCAGTCGCTT CGCCGACTAC 
    TTTGTCATCT GCGGGCTGGA CACCGAGACC GGGCTGGAGC CGGACGAGCT GTCGGCATTA
    TGCCAGTACA TACAGGCTTC TAAAGCCAGG GATGGTGCCA GCTCTTTCAT TTCAAGTACA
    ACTGAAGGAG AAAATTTTGA GCAGACACCG TTGAGACGAA CATTCAAGTC TAAGGTCCTT
    GCACGATATC CTGAGAATGT GGACTGGAAT CCCTTTGACC AAGATGCAGT GGGAATGCTA
    TGCATGCCCA AAGGGCTGGC ATTCAAGACT CAGGCTGATC CCAGGGAGCC TCAGTTCCAT
    GCCTTTATTA TCACTAGGGA GGATGGCTCT CGGACCTTTG GGTTTGCTCT CACGTTTTAT
    GAGGAGGTGA CTAGCAAGCA GATCTGCAGT GCAATGCAGA CCCTCTACCA CATGCATAAT
    GCAGAGTATG ATGTCCTCCA TGCTCCACTT GCTGATAGCC GAGACCTCAG TGGCATGGAG
    GATGGTGATG GCACTCCTGG GACCAAGCTG CAGCGCTTCA ACTCCTACGA CATTAGCAGA
    GATACACTCT ACGTCTCTAA GTGCATCTGC CTCATCACGC CCATGTCCTT CATGAAAGCA
    TGTCGGAGTG TGCTGGAACA GCTTCACCAG GCAGTCACTT CGCCTCAGCC CCCTCCACTG
    CCCCTTGAGA GCTACATATA CAATGTACTA TATGAGGTGC CACTCCCACC TCCTGGCCGG
    TCCTTGAAGT TTTCTGGTGT CTATGGGCCA ATCGTCTGCC AGAGACCAAG TACCAATGAG
    CTTCCCCTGT TCGACTTTCC TGTCAAAGAG GTCTTTGAAC TGCTTGGGGT GGAGAATGTG
    TTTCAACTTT TTACTTGTGC CCTTCTGGAG TTTCAAATCC TGCTCTACTC ACAGCATTAC
    CAGCGACTGA TGACTGTGGC GGAGACAATT ACAGCTCTCA TGTTTCCTTT CCAGTGGCAG
    CATGTCTATG TTCCTATTCT CCCGGCTTCT CTCCTACATT TCTTAGATGC TCCTGTGCCA
    TACTTGATGG GCTTGCATTC CAATGGCTTG GATGACCGGT CAAAACTGGA GCTGCCTCAA
    GAGGCTAACC TCTGCTTTGT GGACATTGAC AACCACTTCA TTGAGTTACC AGAGGACTTG
    CCTCAGTTTC CCAACAAATT GGAGTTTGTC CAGGAAGTCT CTGAGATTCT CATGGCATTT
    GGCATTCCCC CTGAAGGAAG CCTTCACTGT AGCGAGAGTG CTTCCAAGCT GAAGAGGCTC
    CGGGCCTCTG AACTCGTCTC TGACAAGAGG AATGGAAACA TCGCAGGCTC CCCTTTACAT
    TCCTATGAGT TCCTCAAGGA GAATGAAACT ATCGCCCGCC TGCAGGCCTT GGTCAAGAGG
    ACTGGGGTGA GCCTGGAGAA GTTGGAAGTG CGTGAAGACC CCAGTAGCAA TAAGGATCTC
    AAAGTTCAGT GTGATGAAGA AGAACTCAGG ATTTACCAGC TAAACATTCA GATCCGGGAA
    GTTTTTGCAA ATCGTTTCAC TCAGATGTTT GCAGATTATG AGGTGTTTGT CATTCAGCCC
    AGCCAGGATA AGGAGTCCTG GTTTACCAAC AGAGAGCAGA TGCAAAATTT TGATAAAGCA
    TCTTTTCTGT CAGATCAGCC TGAACCCTAC CTGCCTTTCC TCTCAAGATT CTTGGAGACT
    CAGATGTTTG CTTCTTTCAT TGACAACAAA ATCATGTGTC ATGACGATGA TGATAAAGAT
    CCTGTGCTCC GGGTGTTTGA CTCCCGAGTA GACAAGATCA GGCTGTTGAA TGTTCGGACA
    CCTACTCTGC GCACATCCAT GTACCAGAAG TGTACCACAG TGGATGAAGC AAAGAAAGCA
    ATTGAGCTGC GATTGGCAAA AATTGATCAT ACTGCGGTTC ATCCCCATTT ACTTGACATG
    AAGATTGGAC AAGGGAAGTA TGAACCAGGC TTCTTCCCTA AGCTACAGTC TGATGTGCTT
    TCCACTGGGC CAGCCAGCAA CAAGTGGACA AAAAGAAATG CTCCTGCCCA GTGGAGGCGT
    AAAGACCGGC AGAAGCAGCA CACAGAACAC CTGCGCTTAG ACAATGACCA GAGAGAGAAG
    TACATCCAGG AAGCCAGGAA TATGGGCAGC ACCATCCGCC AGCCCAAACT GTCCAACCTC
    TCCCCGTCAG TGATTGCCCA GACCAACTGG AAGTTTGTGG AAGGTCTGCT GAAGGAATGC
    CGCAACAAGA CCAAGAGGAT GCTGGTGGAG AAGATGGGGC GAGAGGCTGT GGAACTAGGC
    CATGGGGAAG TGAACATCAC AGGGGTGGAA GAGAACACCT TGATTGCCAG TCTTTGTGAC
    CTACTGGAAA GGATTTGGAG TCATGGGCTA CAGGTGAAAC AGGGGAAATC AGCCTTGTGG
    TCCCACCTGT TACATTATCA GGAAAACCGG CAGAGAAAAC TCACATCAGG AAGCCTCAGT
    ACCTCAGGAA TATTTCTTGA TTCAGAGCGG AGGAAGTCTG ATGCCAGCTC CGTCATGTCT
    CCCCTGAAAA TCTCCCTCAT TCAGGATATG AGGCACATCC AGAACATTGG GGAAATCAAG
    ACTGATGTGG GAAAAGCCAG AGCGTGGGTG CGTCTGTCCA TGGAAAAAAA GTTACTTTCT
    AGACACCTGA AGCAGCTTCT CTCAGACCAT GAGCTCACCA AAAAACTCTA CAAGCGCTAC
    GCCTTCCTGC GCTGTGACGA CGAGAAGGAG CAGTTCCTCT ATCACCTCCT CTCTTTCAAT
    GCCGTCGATT ACTTCTGCTT CACCAATGTC TTCACGACTA TCCTGATTCC TTACCACATC
    CTGATTGTAC CAAGCAAGAA GCTGGGGGGC TCTATGTTCA CCGCCAACCC ATGGATCTGC
    ATCTCAGGAG AACTGGGTGA GACACAGATC CTGCAGATTC CCAGGAACCT GTTAGAGATG
    ACCTTCGAGT GCCAAAACTT GGGGAAACTC ACTACTGTGC AGATAGGCCA TGATAACTCT
    GGACTCTACG CCAAATGGTT GGTGGAATAT GTGATGGTTA GGAATGAGAT CACCGGACAT
    ACCTACAAGT TTCCATGTGG CCGCTGGTTG GGGAAGGGCA TGGATGACGG GAGCCTGGAG
    CGGGTTCTGG TCGGGGAGCT GCTCACATCC CAGCCTGAGG TGGATGAGAG GCCGTGCCGG
    ACTCCGCCCC TGCAGCAGTC TCCCAGCGTC ATCCGGAGGC TTGTTACCAT CTCACCCAAC
    AACAGACCCA AGCTGAACAC TGGGCAGATC CAAGAGTCCA TCGGTGAGGC AGTCAATGGC
    ATCGTGAAGC ACTTCCATAA GCCTGAAAAA GAGCGAGGTA GTCTGACGCT GTTGCTTTGT
    GGAGAATGTG GCCTTGTCTC GGCCCTGGAA CAGGCTTTCC AGCATGGATT TAAGTCACCA
    CGGCTCTTCA AAAATGTCTT CATTTGGGAT TTTTTGGAAA AAGCACAAAC CTATTATGAG
    ACTTTGGAGC AGAACGAACT TGTCCCTGAG GAAAACTGGC ATATGAGGGC CCGGAACTTC
    TGCCGATTTG TCACGGCAAT CAACAATAAT CCCCGGAACA TTGGCAAGGA TGAGACCACC
    TCCTGCACCA CTGGATCGCC CTGCTGGCTG ACTGCCCCAT CACCGCACAC ATGTATGAGG
    ATGTGGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57545
    Clone ID Related Accession (Same CDS sequence) XM_010822806.2 , XM_010812548.2
    Accession Version XM_010822806.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DENN domain-containing protein 5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822806.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGCGGCG GCGGCGGCGG CGGCGGAGGG GGCTCGGCGC CCAGTCGCTT CGCCGACTAC 
    TTTGTCATCT GCGGGCTGGA CACCGAGACC GGGCTGGAGC CGGACGAGCT GTCGGGAGAA
    AATTTTGAGC AGACACCGTT GAGACGAACA TTCAAGTCTA AGGTCCTTGC ACGATATCCT
    GAGAATGTGG ACTGGAATCC CTTTGACCAA GATGCAGTGG GAATGCTATG CATGCCCAAA
    GGGCTGGCAT TCAAGACTCA GGCTGATCCC AGGGAGCCTC AGTTCCATGC CTTTATTATC
    ACTAGGGAGG ATGGCTCTCG GACCTTTGGG TTTGCTCTCA CGTTTTATGA GGAGGTGACT
    AGCAAGCAGA TCTGCAGTGC AATGCAGACC CTCTACCACA TGCATAATGC AGAGTATGAT
    GTCCTCCATG CTCCACTTGC TGATAGCCGA GACCTCAGTG GCATGGAGGA TGGTGATGGC
    ACTCCTGGGA CCAAGCTGCA GCGCTTCAAC TCCTACGACA TTAGCAGAGA TACACTCTAC
    GTCTCTAAGT GCATCTGCCT CATCACGCCC ATGTCCTTCA TGAAAGCATG TCGGAGTGTG
    CTGGAACAGC TTCACCAGGC AGTCACTTCG CCTCAGCCCC CTCCACTGCC CCTTGAGAGC
    TACATATACA ATGTACTATA TGAGGTGCCA CTCCCACCTC CTGGCCGGTC CTTGAAGTTT
    TCTGGTGTCT ATGGGCCAAT CGTCTGCCAG AGACCAAGTA CCAATGAGCT TCCCCTGTTC
    GACTTTCCTG TCAAAGAGGT CTTTGAACTG CTTGGGGTGG AGAATGTGTT TCAACTTTTT
    ACTTGTGCCC TTCTGGAGTT TCAAATCCTG CTCTACTCAC AGCATTACCA GCGACTGATG
    ACTGTGGCGG AGACAATTAC AGCTCTCATG TTTCCTTTCC AGTGGCAGCA TGTCTATGTT
    CCTATTCTCC CGGCTTCTCT CCTACATTTC TTAGATGCTC CTGTGCCATA CTTGATGGGC
    TTGCATTCCA ATGGCTTGGA TGACCGGTCA AAACTGGAGC TGCCTCAAGA GGCTAACCTC
    TGCTTTGTGG ACATTGACAA CCACTTCATT GAGTTACCAG AGGACTTGCC TCAGTTTCCC
    AACAAATTGG AGTTTGTCCA GGAAGTCTCT GAGATTCTCA TGGCATTTGG CATTCCCCCT
    GAAGGAAGCC TTCACTGTAG CGAGAGTGCT TCCAAGCTGA AGAGGCTCCG GGCCTCTGAA
    CTCGTCTCTG ACAAGAGGAA TGGAAACATC GCAGGCTCCC CTTTACATTC CTATGAGTTC
    CTCAAGGAGA ATGAAACTAT CGCCCGCCTG CAGGCCTTGG TCAAGAGGAC TGGGGTGAGC
    CTGGAGAAGT TGGAAGTGCG TGAAGACCCC AGTAGCAATA AGGATCTCAA AGTTCAGTGT
    GATGAAGAAG AACTCAGGAT TTACCAGCTA AACATTCAGA TCCGGGAAGT TTTTGCAAAT
    CGTTTCACTC AGATGTTTGC AGATTATGAG GTGTTTGTCA TTCAGCCCAG CCAGGATAAG
    GAGTCCTGGT TTACCAACAG AGAGCAGATG CAAAATTTTG ATAAAGCATC TTTTCTGTCA
    GATCAGCCTG AACCCTACCT GCCTTTCCTC TCAAGATTCT TGGAGACTCA GATGTTTGCT
    TCTTTCATTG ACAACAAAAT CATGTGTCAT GACGATGATG ATAAAGATCC TGTGCTCCGG
    GTGTTTGACT CCCGAGTAGA CAAGATCAGG CTGTTGAATG TTCGGACACC TACTCTGCGC
    ACATCCATGT ACCAGAAGTG TACCACAGTG GATGAAGCAA AGAAAGCAAT TGAGCTGCGA
    TTGGCAAAAA TTGATCATAC TGCGGTTCAT CCCCATTTAC TTGACATGAA GATTGGACAA
    GGGAAGTATG AACCAGGCTT CTTCCCTAAG CTACAGTCTG ATGTGCTTTC CACTGGGCCA
    GCCAGCAACA AGTGGACAAA AAGAAATGCT CCTGCCCAGT GGAGGCGTAA AGACCGGCAG
    AAGCAGCACA CAGAACACCT GCGCTTAGAC AATGACCAGA GAGAGAAGTA CATCCAGGAA
    GCCAGGAATA TGGGCAGCAC CATCCGCCAG CCCAAACTGT CCAACCTCTC CCCGTCAGTG
    ATTGCCCAGA CCAACTGGAA GTTTGTGGAA GGTCTGCTGA AGGAATGCCG CAACAAGACC
    AAGAGGATGC TGGTGGAGAA GATGGGGCGA GAGGCTGTGG AACTAGGCCA TGGGGAAGTG
    AACATCACAG GGGTGGAAGA GAACACCTTG ATTGCCAGTC TTTGTGACCT ACTGGAAAGG
    ATTTGGAGTC ATGGGCTACA GGTGAAACAG GGGAAATCAG CCTTGTGGTC CCACCTGTTA
    CATTATCAGG AAAACCGGCA GAGAAAACTC ACATCAGGAA GCCTCAGTAC CTCAGGAATA
    TTTCTTGATT CAGAGCGGAG GAAGTCTGAT GCCAGCTCCG TCATGTCTCC CCTGAAAATC
    TCCCTCATTC AGGATATGAG GCACATCCAG AACATTGGGG AAATCAAGAC TGATGTGGGA
    AAAGCCAGAG CGTGGGTGCG TCTGTCCATG GAAAAAAAGT TACTTTCTAG ACACCTGAAG
    CAGCTTCTCT CAGACCATGA GCTCACCAAA AAACTCTACA AGCGCTACGC CTTCCTGCGC
    TGTGACGACG AGAAGGAGCA GTTCCTCTAT CACCTCCTCT CTTTCAATGC CGTCGATTAC
    TTCTGCTTCA CCAATGTCTT CACGACTATC CTGATTCCTT ACCACATCCT GATTGTACCA
    AGCAAGAAGC TGGGGGGCTC TATGTTCACC GCCAACCCAT GGATCTGCAT CTCAGGAGAA
    CTGGGTGAGA CACAGATCCT GCAGATTCCC AGGAACCTGT TAGAGATGAC CTTCGAGTGC
    CAAAACTTGG GGAAACTCAC TACTGTGCAG ATAGGCCATG ATAACTCTGG ACTCTACGCC
    AAATGGTTGG TGGAATATGT GATGGTTAGG AATGAGATCA CCGGACATAC CTACAAGTTT
    CCATGTGGCC GCTGGTTGGG GAAGGGCATG GATGACGGGA GCCTGGAGCG GGTTCTGGTC
    GGGGAGCTGC TCACATCCCA GCCTGAGGTG GATGAGAGGC CGTGCCGGAC TCCGCCCCTG
    CAGCAGTCTC CCAGCGTCAT CCGGAGGCTT GTTACCATCT CACCCAACAA CAGACCCAAG
    CTGAACACTG GGCAGATCCA AGAGTCCATC GGTGAGGCAG TCAATGGCAT CGTGAAGCAC
    TTCCATAAGC CTGAAAAAGA GCGAGGTAGT CTGACGCTGT TGCTTTGTGG AGAATGTGGC
    CTTGTCTCGG CCCTGGAACA GGCTTTCCAG CATGGATTTA AGTCACCACG GCTCTTCAAA
    AATGTCTTCA TTTGGGATTT TTTGGAAAAA GCACAAACCT ATTATGAGAC TTTGGAGCAG
    AACGAACTTG TCCCTGAGGA AAACTGGCAT ATGAGGGCCC GGAACTTCTG CCGATTTGTC
    ACGGCAATCA ACAATAATCC CCGGAACATT GGCAAGGATG GCAAGTTTCA GATGCTGGTG
    TGCTTGGGAG CCAGAGACCA CCTCCTGCAC CACTGGATCG CCCTGCTGGC TGACTGCCCC
    ATCACCGCAC ACATGTATGA GGATGTGGCA CTGATCAAAG ACCACACACT TGTCAATTCC
    TTGATCCGCG TGCTGCAGAC ACTGCAGGAG TTCAACATCA CGCTGGAGAC ATCTCTGGTC
    AAGGGCATCG ATATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821108.2
    CDS19..3816
    Translation

    Target ORF information:

    RefSeq Version XM_010822806.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DENN/MADD domain containing 5A (DENND5A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010822806.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGCGGCG GCGGCGGCGG CGGCGGAGGG GGCTCGGCGC CCAGTCGCTT CGCCGACTAC 
    TTTGTCATCT GCGGGCTGGA CACCGAGACC GGGCTGGAGC CGGACGAGCT GTCGGGAGAA
    AATTTTGAGC AGACACCGTT GAGACGAACA TTCAAGTCTA AGGTCCTTGC ACGATATCCT
    GAGAATGTGG ACTGGAATCC CTTTGACCAA GATGCAGTGG GAATGCTATG CATGCCCAAA
    GGGCTGGCAT TCAAGACTCA GGCTGATCCC AGGGAGCCTC AGTTCCATGC CTTTATTATC
    ACTAGGGAGG ATGGCTCTCG GACCTTTGGG TTTGCTCTCA CGTTTTATGA GGAGGTGACT
    AGCAAGCAGA TCTGCAGTGC AATGCAGACC CTCTACCACA TGCATAATGC AGAGTATGAT
    GTCCTCCATG CTCCACTTGC TGATAGCCGA GACCTCAGTG GCATGGAGGA TGGTGATGGC
    ACTCCTGGGA CCAAGCTGCA GCGCTTCAAC TCCTACGACA TTAGCAGAGA TACACTCTAC
    GTCTCTAAGT GCATCTGCCT CATCACGCCC ATGTCCTTCA TGAAAGCATG TCGGAGTGTG
    CTGGAACAGC TTCACCAGGC AGTCACTTCG CCTCAGCCCC CTCCACTGCC CCTTGAGAGC
    TACATATACA ATGTACTATA TGAGGTGCCA CTCCCACCTC CTGGCCGGTC CTTGAAGTTT
    TCTGGTGTCT ATGGGCCAAT CGTCTGCCAG AGACCAAGTA CCAATGAGCT TCCCCTGTTC
    GACTTTCCTG TCAAAGAGGT CTTTGAACTG CTTGGGGTGG AGAATGTGTT TCAACTTTTT
    ACTTGTGCCC TTCTGGAGTT TCAAATCCTG CTCTACTCAC AGCATTACCA GCGACTGATG
    ACTGTGGCGG AGACAATTAC AGCTCTCATG TTTCCTTTCC AGTGGCAGCA TGTCTATGTT
    CCTATTCTCC CGGCTTCTCT CCTACATTTC TTAGATGCTC CTGTGCCATA CTTGATGGGC
    TTGCATTCCA ATGGCTTGGA TGACCGGTCA AAACTGGAGC TGCCTCAAGA GGCTAACCTC
    TGCTTTGTGG ACATTGACAA CCACTTCATT GAGTTACCAG AGGACTTGCC TCAGTTTCCC
    AACAAATTGG AGTTTGTCCA GGAAGTCTCT GAGATTCTCA TGGCATTTGG CATTCCCCCT
    GAAGGAAGCC TTCACTGTAG CGAGAGTGCT TCCAAGCTGA AGAGGCTCCG GGCCTCTGAA
    CTCGTCTCTG ACAAGAGGAA TGGAAACATC GCAGGCTCCC CTTTACATTC CTATGAGTTC
    CTCAAGGAGA ATGAAACTAT CGCCCGCCTG CAGGCCTTGG TCAAGAGGAC TGGGGTGAGC
    CTGGAGAAGT TGGAAGTGCG TGAAGACCCC AGTAGCAATA AGGATCTCAA AGTTCAGTGT
    GATGAAGAAG AACTCAGGAT TTACCAGCTA AACATTCAGA TCCGGGAAGT TTTTGCAAAT
    CGTTTCACTC AGATGTTTGC AGATTATGAG GTGTTTGTCA TTCAGCCCAG CCAGGATAAG
    GAGTCCTGGT TTACCAACAG AGAGCAGATG CAAAATTTTG ATAAAGCATC TTTTCTGTCA
    GATCAGCCTG AACCCTACCT GCCTTTCCTC TCAAGATTCT TGGAGACTCA GATGTTTGCT
    TCTTTCATTG ACAACAAAAT CATGTGTCAT GACGATGATG ATAAAGATCC TGTGCTCCGG
    GTGTTTGACT CCCGAGTAGA CAAGATCAGG CTGTTGAATG TTCGGACACC TACTCTGCGC
    ACATCCATGT ACCAGAAGTG TACCACAGTG GATGAAGCAA AGAAAGCAAT TGAGCTGCGA
    TTGGCAAAAA TTGATCATAC TGCGGTTCAT CCCCATTTAC TTGACATGAA GATTGGACAA
    GGGAAGTATG AACCAGGCTT CTTCCCTAAG CTACAGTCTG ATGTGCTTTC CACTGGGCCA
    GCCAGCAACA AGTGGACAAA AAGAAATGCT CCTGCCCAGT GGAGGCGTAA AGACCGGCAG
    AAGCAGCACA CAGAACACCT GCGCTTAGAC AATGACCAGA GAGAGAAGTA CATCCAGGAA
    GCCAGGAATA TGGGCAGCAC CATCCGCCAG CCCAAACTGT CCAACCTCTC CCCGTCAGTG
    ATTGCCCAGA CCAACTGGAA GTTTGTGGAA GGTCTGCTGA AGGAATGCCG CAACAAGACC
    AAGAGGATGC TGGTGGAGAA GATGGGGCGA GAGGCTGTGG AACTAGGCCA TGGGGAAGTG
    AACATCACAG GGGTGGAAGA GAACACCTTG ATTGCCAGTC TTTGTGACCT ACTGGAAAGG
    ATTTGGAGTC ATGGGCTACA GGTGAAACAG GGGAAATCAG CCTTGTGGTC CCACCTGTTA
    CATTATCAGG AAAACCGGCA GAGAAAACTC ACATCAGGAA GCCTCAGTAC CTCAGGAATA
    TTTCTTGATT CAGAGCGGAG GAAGTCTGAT GCCAGCTCCG TCATGTCTCC CCTGAAAATC
    TCCCTCATTC AGGATATGAG GCACATCCAG AACATTGGGG AAATCAAGAC TGATGTGGGA
    AAAGCCAGAG CGTGGGTGCG TCTGTCCATG GAAAAAAAGT TACTTTCTAG ACACCTGAAG
    CAGCTTCTCT CAGACCATGA GCTCACCAAA AAACTCTACA AGCGCTACGC CTTCCTGCGC
    TGTGACGACG AGAAGGAGCA GTTCCTCTAT CACCTCCTCT CTTTCAATGC CGTCGATTAC
    TTCTGCTTCA CCAATGTCTT CACGACTATC CTGATTCCTT ACCACATCCT GATTGTACCA
    AGCAAGAAGC TGGGGGGCTC TATGTTCACC GCCAACCCAT GGATCTGCAT CTCAGGAGAA
    CTGGGTGAGA CACAGATCCT GCAGATTCCC AGGAACCTGT TAGAGATGAC CTTCGAGTGC
    CAAAACTTGG GGAAACTCAC TACTGTGCAG ATAGGCCATG ATAACTCTGG ACTCTACGCC
    AAATGGTTGG TGGAATATGT GATGGTTAGG AATGAGATCA CCGGACATAC CTACAAGTTT
    CCATGTGGCC GCTGGTTGGG GAAGGGCATG GATGACGGGA GCCTGGAGCG GGTTCTGGTC
    GGGGAGCTGC TCACATCCCA GCCTGAGGTG GATGAGAGGC CGTGCCGGAC TCCGCCCCTG
    CAGCAGTCTC CCAGCGTCAT CCGGAGGCTT GTTACCATCT CACCCAACAA CAGACCCAAG
    CTGAACACTG GGCAGATCCA AGAGTCCATC GGTGAGGCAG TCAATGGCAT CGTGAAGCAC
    TTCCATAAGC CTGAAAAAGA GCGAGGTAGT CTGACGCTGT TGCTTTGTGG AGAATGTGGC
    CTTGTCTCGG CCCTGGAACA GGCTTTCCAG CATGGATTTA AGTCACCACG GCTCTTCAAA
    AATGTCTTCA TTTGGGATTT TTTGGAAAAA GCACAAACCT ATTATGAGAC TTTGGAGCAG
    AACGAACTTG TCCCTGAGGA AAACTGGCAT ATGAGGGCCC GGAACTTCTG CCGATTTGTC
    ACGGCAATCA ACAATAATCC CCGGAACATT GGCAAGGATG GCAAGTTTCA GATGCTGGTG
    TGCTTGGGAG CCAGAGACCA CCTCCTGCAC CACTGGATCG CCCTGCTGGC TGACTGCCCC
    ATCACCGCAC ACATGTATGA GGATGTGGCA CTGATCAAAG ACCACACACT TGTCAATTCC
    TTGATCCGCG TGCTGCAGAC ACTGCAGGAG TTCAACATCA CGCTGGAGAC ATCTCTGGTC
    AAGGGCATCG ATATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57545
    Clone ID Related Accession (Same CDS sequence) XM_010822806.2 , XM_010812548.2
    Accession Version XM_010812548.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DENN domain-containing protein 5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812548.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGCGGCG GCGGCGGCGG CGGCGGAGGG GGCTCGGCGC CCAGTCGCTT CGCCGACTAC 
    TTTGTCATCT GCGGGCTGGA CACCGAGACC GGGCTGGAGC CGGACGAGCT GTCGGGAGAA
    AATTTTGAGC AGACACCGTT GAGACGAACA TTCAAGTCTA AGGTCCTTGC ACGATATCCT
    GAGAATGTGG ACTGGAATCC CTTTGACCAA GATGCAGTGG GAATGCTATG CATGCCCAAA
    GGGCTGGCAT TCAAGACTCA GGCTGATCCC AGGGAGCCTC AGTTCCATGC CTTTATTATC
    ACTAGGGAGG ATGGCTCTCG GACCTTTGGG TTTGCTCTCA CGTTTTATGA GGAGGTGACT
    AGCAAGCAGA TCTGCAGTGC AATGCAGACC CTCTACCACA TGCATAATGC AGAGTATGAT
    GTCCTCCATG CTCCACTTGC TGATAGCCGA GACCTCAGTG GCATGGAGGA TGGTGATGGC
    ACTCCTGGGA CCAAGCTGCA GCGCTTCAAC TCCTACGACA TTAGCAGAGA TACACTCTAC
    GTCTCTAAGT GCATCTGCCT CATCACGCCC ATGTCCTTCA TGAAAGCATG TCGGAGTGTG
    CTGGAACAGC TTCACCAGGC AGTCACTTCG CCTCAGCCCC CTCCACTGCC CCTTGAGAGC
    TACATATACA ATGTACTATA TGAGGTGCCA CTCCCACCTC CTGGCCGGTC CTTGAAGTTT
    TCTGGTGTCT ATGGGCCAAT CGTCTGCCAG AGACCAAGTA CCAATGAGCT TCCCCTGTTC
    GACTTTCCTG TCAAAGAGGT CTTTGAACTG CTTGGGGTGG AGAATGTGTT TCAACTTTTT
    ACTTGTGCCC TTCTGGAGTT TCAAATCCTG CTCTACTCAC AGCATTACCA GCGACTGATG
    ACTGTGGCGG AGACAATTAC AGCTCTCATG TTTCCTTTCC AGTGGCAGCA TGTCTATGTT
    CCTATTCTCC CGGCTTCTCT CCTACATTTC TTAGATGCTC CTGTGCCATA CTTGATGGGC
    TTGCATTCCA ATGGCTTGGA TGACCGGTCA AAACTGGAGC TGCCTCAAGA GGCTAACCTC
    TGCTTTGTGG ACATTGACAA CCACTTCATT GAGTTACCAG AGGACTTGCC TCAGTTTCCC
    AACAAATTGG AGTTTGTCCA GGAAGTCTCT GAGATTCTCA TGGCATTTGG CATTCCCCCT
    GAAGGAAGCC TTCACTGTAG CGAGAGTGCT TCCAAGCTGA AGAGGCTCCG GGCCTCTGAA
    CTCGTCTCTG ACAAGAGGAA TGGAAACATC GCAGGCTCCC CTTTACATTC CTATGAGTTC
    CTCAAGGAGA ATGAAACTAT CGCCCGCCTG CAGGCCTTGG TCAAGAGGAC TGGGGTGAGC
    CTGGAGAAGT TGGAAGTGCG TGAAGACCCC AGTAGCAATA AGGATCTCAA AGTTCAGTGT
    GATGAAGAAG AACTCAGGAT TTACCAGCTA AACATTCAGA TCCGGGAAGT TTTTGCAAAT
    CGTTTCACTC AGATGTTTGC AGATTATGAG GTGTTTGTCA TTCAGCCCAG CCAGGATAAG
    GAGTCCTGGT TTACCAACAG AGAGCAGATG CAAAATTTTG ATAAAGCATC TTTTCTGTCA
    GATCAGCCTG AACCCTACCT GCCTTTCCTC TCAAGATTCT TGGAGACTCA GATGTTTGCT
    TCTTTCATTG ACAACAAAAT CATGTGTCAT GACGATGATG ATAAAGATCC TGTGCTCCGG
    GTGTTTGACT CCCGAGTAGA CAAGATCAGG CTGTTGAATG TTCGGACACC TACTCTGCGC
    ACATCCATGT ACCAGAAGTG TACCACAGTG GATGAAGCAA AGAAAGCAAT TGAGCTGCGA
    TTGGCAAAAA TTGATCATAC TGCGGTTCAT CCCCATTTAC TTGACATGAA GATTGGACAA
    GGGAAGTATG AACCAGGCTT CTTCCCTAAG CTACAGTCTG ATGTGCTTTC CACTGGGCCA
    GCCAGCAACA AGTGGACAAA AAGAAATGCT CCTGCCCAGT GGAGGCGTAA AGACCGGCAG
    AAGCAGCACA CAGAACACCT GCGCTTAGAC AATGACCAGA GAGAGAAGTA CATCCAGGAA
    GCCAGGAATA TGGGCAGCAC CATCCGCCAG CCCAAACTGT CCAACCTCTC CCCGTCAGTG
    ATTGCCCAGA CCAACTGGAA GTTTGTGGAA GGTCTGCTGA AGGAATGCCG CAACAAGACC
    AAGAGGATGC TGGTGGAGAA GATGGGGCGA GAGGCTGTGG AACTAGGCCA TGGGGAAGTG
    AACATCACAG GGGTGGAAGA GAACACCTTG ATTGCCAGTC TTTGTGACCT ACTGGAAAGG
    ATTTGGAGTC ATGGGCTACA GGTGAAACAG GGGAAATCAG CCTTGTGGTC CCACCTGTTA
    CATTATCAGG AAAACCGGCA GAGAAAACTC ACATCAGGAA GCCTCAGTAC CTCAGGAATA
    TTTCTTGATT CAGAGCGGAG GAAGTCTGAT GCCAGCTCCG TCATGTCTCC CCTGAAAATC
    TCCCTCATTC AGGATATGAG GCACATCCAG AACATTGGGG AAATCAAGAC TGATGTGGGA
    AAAGCCAGAG CGTGGGTGCG TCTGTCCATG GAAAAAAAGT TACTTTCTAG ACACCTGAAG
    CAGCTTCTCT CAGACCATGA GCTCACCAAA AAACTCTACA AGCGCTACGC CTTCCTGCGC
    TGTGACGACG AGAAGGAGCA GTTCCTCTAT CACCTCCTCT CTTTCAATGC CGTCGATTAC
    TTCTGCTTCA CCAATGTCTT CACGACTATC CTGATTCCTT ACCACATCCT GATTGTACCA
    AGCAAGAAGC TGGGGGGCTC TATGTTCACC GCCAACCCAT GGATCTGCAT CTCAGGAGAA
    CTGGGTGAGA CACAGATCCT GCAGATTCCC AGGAACCTGT TAGAGATGAC CTTCGAGTGC
    CAAAACTTGG GGAAACTCAC TACTGTGCAG ATAGGCCATG ATAACTCTGG ACTCTACGCC
    AAATGGTTGG TGGAATATGT GATGGTTAGG AATGAGATCA CCGGACATAC CTACAAGTTT
    CCATGTGGCC GCTGGTTGGG GAAGGGCATG GATGACGGGA GCCTGGAGCG GGTTCTGGTC
    GGGGAGCTGC TCACATCCCA GCCTGAGGTG GATGAGAGGC CGTGCCGGAC TCCGCCCCTG
    CAGCAGTCTC CCAGCGTCAT CCGGAGGCTT GTTACCATCT CACCCAACAA CAGACCCAAG
    CTGAACACTG GGCAGATCCA AGAGTCCATC GGTGAGGCAG TCAATGGCAT CGTGAAGCAC
    TTCCATAAGC CTGAAAAAGA GCGAGGTAGT CTGACGCTGT TGCTTTGTGG AGAATGTGGC
    CTTGTCTCGG CCCTGGAACA GGCTTTCCAG CATGGATTTA AGTCACCACG GCTCTTCAAA
    AATGTCTTCA TTTGGGATTT TTTGGAAAAA GCACAAACCT ATTATGAGAC TTTGGAGCAG
    AACGAACTTG TCCCTGAGGA AAACTGGCAT ATGAGGGCCC GGAACTTCTG CCGATTTGTC
    ACGGCAATCA ACAATAATCC CCGGAACATT GGCAAGGATG GCAAGTTTCA GATGCTGGTG
    TGCTTGGGAG CCAGAGACCA CCTCCTGCAC CACTGGATCG CCCTGCTGGC TGACTGCCCC
    ATCACCGCAC ACATGTATGA GGATGTGGCA CTGATCAAAG ACCACACACT TGTCAATTCC
    TTGATCCGCG TGCTGCAGAC ACTGCAGGAG TTCAACATCA CGCTGGAGAC ATCTCTGGTC
    AAGGGCATCG ATATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810850.1
    CDS19..3816
    Translation

    Target ORF information:

    RefSeq Version XM_010812548.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DENN/MADD domain containing 5A (DENND5A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812548.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGCGGCG GCGGCGGCGG CGGCGGAGGG GGCTCGGCGC CCAGTCGCTT CGCCGACTAC 
    TTTGTCATCT GCGGGCTGGA CACCGAGACC GGGCTGGAGC CGGACGAGCT GTCGGGAGAA
    AATTTTGAGC AGACACCGTT GAGACGAACA TTCAAGTCTA AGGTCCTTGC ACGATATCCT
    GAGAATGTGG ACTGGAATCC CTTTGACCAA GATGCAGTGG GAATGCTATG CATGCCCAAA
    GGGCTGGCAT TCAAGACTCA GGCTGATCCC AGGGAGCCTC AGTTCCATGC CTTTATTATC
    ACTAGGGAGG ATGGCTCTCG GACCTTTGGG TTTGCTCTCA CGTTTTATGA GGAGGTGACT
    AGCAAGCAGA TCTGCAGTGC AATGCAGACC CTCTACCACA TGCATAATGC AGAGTATGAT
    GTCCTCCATG CTCCACTTGC TGATAGCCGA GACCTCAGTG GCATGGAGGA TGGTGATGGC
    ACTCCTGGGA CCAAGCTGCA GCGCTTCAAC TCCTACGACA TTAGCAGAGA TACACTCTAC
    GTCTCTAAGT GCATCTGCCT CATCACGCCC ATGTCCTTCA TGAAAGCATG TCGGAGTGTG
    CTGGAACAGC TTCACCAGGC AGTCACTTCG CCTCAGCCCC CTCCACTGCC CCTTGAGAGC
    TACATATACA ATGTACTATA TGAGGTGCCA CTCCCACCTC CTGGCCGGTC CTTGAAGTTT
    TCTGGTGTCT ATGGGCCAAT CGTCTGCCAG AGACCAAGTA CCAATGAGCT TCCCCTGTTC
    GACTTTCCTG TCAAAGAGGT CTTTGAACTG CTTGGGGTGG AGAATGTGTT TCAACTTTTT
    ACTTGTGCCC TTCTGGAGTT TCAAATCCTG CTCTACTCAC AGCATTACCA GCGACTGATG
    ACTGTGGCGG AGACAATTAC AGCTCTCATG TTTCCTTTCC AGTGGCAGCA TGTCTATGTT
    CCTATTCTCC CGGCTTCTCT CCTACATTTC TTAGATGCTC CTGTGCCATA CTTGATGGGC
    TTGCATTCCA ATGGCTTGGA TGACCGGTCA AAACTGGAGC TGCCTCAAGA GGCTAACCTC
    TGCTTTGTGG ACATTGACAA CCACTTCATT GAGTTACCAG AGGACTTGCC TCAGTTTCCC
    AACAAATTGG AGTTTGTCCA GGAAGTCTCT GAGATTCTCA TGGCATTTGG CATTCCCCCT
    GAAGGAAGCC TTCACTGTAG CGAGAGTGCT TCCAAGCTGA AGAGGCTCCG GGCCTCTGAA
    CTCGTCTCTG ACAAGAGGAA TGGAAACATC GCAGGCTCCC CTTTACATTC CTATGAGTTC
    CTCAAGGAGA ATGAAACTAT CGCCCGCCTG CAGGCCTTGG TCAAGAGGAC TGGGGTGAGC
    CTGGAGAAGT TGGAAGTGCG TGAAGACCCC AGTAGCAATA AGGATCTCAA AGTTCAGTGT
    GATGAAGAAG AACTCAGGAT TTACCAGCTA AACATTCAGA TCCGGGAAGT TTTTGCAAAT
    CGTTTCACTC AGATGTTTGC AGATTATGAG GTGTTTGTCA TTCAGCCCAG CCAGGATAAG
    GAGTCCTGGT TTACCAACAG AGAGCAGATG CAAAATTTTG ATAAAGCATC TTTTCTGTCA
    GATCAGCCTG AACCCTACCT GCCTTTCCTC TCAAGATTCT TGGAGACTCA GATGTTTGCT
    TCTTTCATTG ACAACAAAAT CATGTGTCAT GACGATGATG ATAAAGATCC TGTGCTCCGG
    GTGTTTGACT CCCGAGTAGA CAAGATCAGG CTGTTGAATG TTCGGACACC TACTCTGCGC
    ACATCCATGT ACCAGAAGTG TACCACAGTG GATGAAGCAA AGAAAGCAAT TGAGCTGCGA
    TTGGCAAAAA TTGATCATAC TGCGGTTCAT CCCCATTTAC TTGACATGAA GATTGGACAA
    GGGAAGTATG AACCAGGCTT CTTCCCTAAG CTACAGTCTG ATGTGCTTTC CACTGGGCCA
    GCCAGCAACA AGTGGACAAA AAGAAATGCT CCTGCCCAGT GGAGGCGTAA AGACCGGCAG
    AAGCAGCACA CAGAACACCT GCGCTTAGAC AATGACCAGA GAGAGAAGTA CATCCAGGAA
    GCCAGGAATA TGGGCAGCAC CATCCGCCAG CCCAAACTGT CCAACCTCTC CCCGTCAGTG
    ATTGCCCAGA CCAACTGGAA GTTTGTGGAA GGTCTGCTGA AGGAATGCCG CAACAAGACC
    AAGAGGATGC TGGTGGAGAA GATGGGGCGA GAGGCTGTGG AACTAGGCCA TGGGGAAGTG
    AACATCACAG GGGTGGAAGA GAACACCTTG ATTGCCAGTC TTTGTGACCT ACTGGAAAGG
    ATTTGGAGTC ATGGGCTACA GGTGAAACAG GGGAAATCAG CCTTGTGGTC CCACCTGTTA
    CATTATCAGG AAAACCGGCA GAGAAAACTC ACATCAGGAA GCCTCAGTAC CTCAGGAATA
    TTTCTTGATT CAGAGCGGAG GAAGTCTGAT GCCAGCTCCG TCATGTCTCC CCTGAAAATC
    TCCCTCATTC AGGATATGAG GCACATCCAG AACATTGGGG AAATCAAGAC TGATGTGGGA
    AAAGCCAGAG CGTGGGTGCG TCTGTCCATG GAAAAAAAGT TACTTTCTAG ACACCTGAAG
    CAGCTTCTCT CAGACCATGA GCTCACCAAA AAACTCTACA AGCGCTACGC CTTCCTGCGC
    TGTGACGACG AGAAGGAGCA GTTCCTCTAT CACCTCCTCT CTTTCAATGC CGTCGATTAC
    TTCTGCTTCA CCAATGTCTT CACGACTATC CTGATTCCTT ACCACATCCT GATTGTACCA
    AGCAAGAAGC TGGGGGGCTC TATGTTCACC GCCAACCCAT GGATCTGCAT CTCAGGAGAA
    CTGGGTGAGA CACAGATCCT GCAGATTCCC AGGAACCTGT TAGAGATGAC CTTCGAGTGC
    CAAAACTTGG GGAAACTCAC TACTGTGCAG ATAGGCCATG ATAACTCTGG ACTCTACGCC
    AAATGGTTGG TGGAATATGT GATGGTTAGG AATGAGATCA CCGGACATAC CTACAAGTTT
    CCATGTGGCC GCTGGTTGGG GAAGGGCATG GATGACGGGA GCCTGGAGCG GGTTCTGGTC
    GGGGAGCTGC TCACATCCCA GCCTGAGGTG GATGAGAGGC CGTGCCGGAC TCCGCCCCTG
    CAGCAGTCTC CCAGCGTCAT CCGGAGGCTT GTTACCATCT CACCCAACAA CAGACCCAAG
    CTGAACACTG GGCAGATCCA AGAGTCCATC GGTGAGGCAG TCAATGGCAT CGTGAAGCAC
    TTCCATAAGC CTGAAAAAGA GCGAGGTAGT CTGACGCTGT TGCTTTGTGG AGAATGTGGC
    CTTGTCTCGG CCCTGGAACA GGCTTTCCAG CATGGATTTA AGTCACCACG GCTCTTCAAA
    AATGTCTTCA TTTGGGATTT TTTGGAAAAA GCACAAACCT ATTATGAGAC TTTGGAGCAG
    AACGAACTTG TCCCTGAGGA AAACTGGCAT ATGAGGGCCC GGAACTTCTG CCGATTTGTC
    ACGGCAATCA ACAATAATCC CCGGAACATT GGCAAGGATG GCAAGTTTCA GATGCTGGTG
    TGCTTGGGAG CCAGAGACCA CCTCCTGCAC CACTGGATCG CCCTGCTGGC TGACTGCCCC
    ATCACCGCAC ACATGTATGA GGATGTGGCA CTGATCAAAG ACCACACACT TGTCAATTCC
    TTGATCCGCG TGCTGCAGAC ACTGCAGGAG TTCAACATCA CGCTGGAGAC ATCTCTGGTC
    AAGGGCATCG ATATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.