PIGO cDNA ORF clone, Bos taurus(cattle)

The following PIGO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIGO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa237446 XM_024996196.1
Latest version!
Bos taurus phosphatidylinositol glycan anchor biosynthesis class O (PIGO), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa05813 XM_002689674.5
Latest version!
Bos taurus phosphatidylinositol glycan anchor biosynthesis class O (PIGO), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa05813 XM_024996194.1
Latest version!
Bos taurus phosphatidylinositol glycan anchor biosynthesis class O (PIGO), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa05813 XM_024996195.1
Latest version!
Bos taurus phosphatidylinositol glycan anchor biosynthesis class O (PIGO), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa91283 XM_001251645.6
Latest version!
Bos taurus phosphatidylinositol glycan anchor biosynthesis class O (PIGO), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa91283 XM_002689674.4
Latest version!
Bos taurus phosphatidylinositol glycan anchor biosynthesis class O (PIGO), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa237446
    Clone ID Related Accession (Same CDS sequence) XM_024996196.1
    Accession Version XM_024996196.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GPI ethanolamine phosphate transferase 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATTCCC TCTCCTGCCA CTACCAAGAT GCAGAAGATC TCAGTGCTGC TTTTCCTGGC 
    CTGGGAAGAC GTGTGGTCTT CATGGGAGAT GAAACCTGGA ATGATCTTTT CCCTGGAGTT
    TTCTCCCAAG CTTTATTCTT CCCATCCTTC AATGTCAGAG ACCTAGAGAC AGTGGACAAT
    GGCATCATGA AACACCTCTA TCCAACCATG GACAGTGACG AATGGGACGT GCTGATTACT
    CACTTCCTGG GTGTGGATCA CTGTGGCCAC AAGCATGACC CTTACCATCC TGAAATGGCT
    AAGAAACTTA CTCAAATGGA TAAAATGATC CAGGGACTTG TGAAGCGTCT GAAGAACGAC
    ACACTGCTGG TGGTGACTGG GGACCATGGG ATGACCATCA GTGGGAACCA TGGAGGAGAG
    AGTGAGCTGG AAACCTCAGC TGCACTTTTT CTATATAGTC CCAGAGCTCT CTTCCCCAGC
    GCTCCTGCAG AGGAGCCAGA GATAATTCCT CAAATCAGCC TTGTACCTAC GCTGGCCCTG
    CTGCTGGGCC TGCCCATCCC ATTTGGAAAC ATCGGGGAGG TGATAGCTGA GGTGTTCTCA
    GAGATCGAAG ACTCCCAGCC TCGCTTCTCT GCTCTGGCCC AAGCCTCAGC TCTGCATCTC
    AATGCCCAGC AGGTGTCCCG ATTTCTTCAC ACCTACTCAG CCACTGCTCA GGACCTTCAA
    GTTCAGGAGC TGCATCGATT GCAGAAGCTC TTCTCCAAGG CTTCTGCTGA CTACCAGCGA
    CTTCTGCAGA GCCCCCAGGG GGCTGAGGCA GTGCTCCAAA CTGTGATTCC TGAGCTGCAG
    CAGTTCCTGC GGGGAGTTCG GGCCATGTGC ATTGAGTCTT GGGCTCGTTT TTCTCTGGTC
    CGCATGGCAG GGGGTGCTGC TCTTCTGGCT GCTACCTGCT TTCTTTGCCT ACTGGTATCC
    CAGTGGGCAA CATCCCCAGC CTTCTATTTC GGCCCTCTCC TAAAACCCAC AGCCTCGGGT
    CTGAGTGGCG CCATGGTGTG TGCTGGACTC CTGGCATCTA CTGGGCTGAA GCTGGATTCC
    ATGGTTCTAG GGGCCATGGC TGCAGTGGGC TCACTCCTGC CTTTTCTGTG GAAAGCTTGG
    GCGAGCTGGG GATCGAAAAG GCCCCTGGCC TCTCTCCTTC CCATGCCCGG GCCAGTCCTA
    TTACTCCTGC TCATTCGCTT TGCTGGTTTC TTCTCTGATA GCTATATTAT AACCGAAGCC
    AGGGTTGCCC CCTTCCTTTT GATCTCACTC ATCTTGCTCC TGGTTGCCCG ACTTCACTGG
    GAGGGCAAGC TGCTGCCACC TAAGCTACTC TCGATACCTC GCCTTGGCTT TTTGACTCCA
    GCAGGTCCCC CGCAATACAG TGCCATGCAT GCCGTGGGAC TTGGAGTTGG GTTGCTTTTA
    TGTATAAGGC TAGCTGGACT TTTTTATCGC TGCCATGAAG AGACACCTGT TTGTAGCTCC
    TCTCCCTTGC TGAGTCCCTT GCGATCCACA GTGGATGGTC GAACCAAGAA TTTGTGGTAT
    GGAGCTTGTG TCGGGGCACT GGTGGCCCTG TTAGCCTCTG TGCGGCTGTG GCTTCACTGC
    TATGGTAATC TCAAGAGCCC TGAGCCCCCT GTGCTCTTTG TGCGCTGGGG TCTGCCCCTA
    ATGGGACTGG GCACTGCTGC CTACTGGGCA TTGGCATCAG GGGCAGATGA AGCACCCCCG
    CGTCTCCGGG CCCTGGTCTC TGGGGCATCA GTTATGTTGC CTCGGGCTGT GGCAGGGTTG
    GCCGCTTCAG GGCTCATGCT GCTGCTCTGG AGGCCTGTGA CAGTGCTGGT GAAGGCCACA
    ACAGGTGCTC CAAGGACCAG GACTGTCCTC ACTCCCTTCT CAGGTCCCCC CACTTCTCAG
    GCTGACTTGG ATTATGTGGT TCCTCAAATC TACCGACACA TACAAGAGGA GTTCCGGGGC
    CGGCTAGAGA GGACCAAATC TCAGGGTCTG CTGACGGTGG CTGCCTATCA GTTGGGGAGC
    GTCTACTCAG CTGCTGTGGT TATGGCCCTT ACCCTCTTGG CCTTCCCACT TCTGCTATTG
    CATGCAGAGC GCATTAGCCT TGTGTTCCTG CTTCTGTTTC TGCAGAGCTT CCTTCTCCTG
    CATCTGCTTG CTGCCGGGAT ACCCATCACC ACCCCTGGTC CTTTTACTGT GCCATGGCAT
    GCAGTTTTTG CATGGGCCCT CATGGCCACA CAGACCTTCT ATACCACCGG CCACCAGCCT
    GTCTTTTCAG CTATCCAATG GCATGCAGCC TTCGTGGGAG TCTCAGAGAG TCGTGACTTT
    ACCTGGCTGT CTGCTTTCCT GGTGGGAGCC AACACCTTTG CCTCCCATAT TCTCTTTGCA
    GTAGGTTGCC CGTTGCTCCT GCTCTGGCCC TTTCTGTGTG AGAATCAAGG ATCCCAGAAG
    AGGCGGCAGC CCCCAGGAAA TGAAGCTGAG GCCAGAGTCA GGCCTGAGGA GGAGCAGGAG
    CCACGGATGG AAATGCGGCT CCGGGATGCA CCTCACCACT TCAGTGCAGC ACTGCTGCAG
    CTGGGCCTCA AGTACCTCTT TGTCCTTGGT ATTCAGATTC TGGCCTGTGT CTTGGCAGCC
    TCCATCCTCC GCAGGCATCT CTTGGTCTGG AAGGTGTTTG CCCCCAAGTT CATTTTTGAG
    GCCATGGGCT TCATTGTGAG CAGCGTGGGA CTTTGTCTAG GCATAGCTTT GGTGATGCGA
    GTAGATGGTG CTGTGAGCTC CTGGTTCAGG CAGTTAGTTC TGACCCAACA GAAAGCTGGA
    GAACAATCTA GCCTGGTCTG TACGGGTGCT GGATCATCTG CAAGAAAGGC TCAGCCACAC
    CTCTTACCAC CATGCAGCCA GGACCTACTG GCATCTGGGA CTTCATTATT TTATAATTCA
    AGATCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851964.1
    CDS564..3512
    Translation

    Target ORF information:

    RefSeq Version XM_024996196.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphatidylinositol glycan anchor biosynthesis class O (PIGO), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996196.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATTCCC TCTCCTGCCA CTACCAAGAT GCAGAAGATC TCAGTGCTGC TTTTCCTGGC 
    CTGGGAAGAC GTGTGGTCTT CATGGGAGAT GAAACCTGGA ATGATCTTTT CCCTGGAGTT
    TTCTCCCAAG CTTTATTCTT CCCATCCTTC AATGTCAGAG ACCTAGAGAC AGTGGACAAT
    GGCATCATGA AACACCTCTA TCCAACCATG GACAGTGACG AATGGGACGT GCTGATTACT
    CACTTCCTGG GTGTGGATCA CTGTGGCCAC AAGCATGACC CTTACCATCC TGAAATGGCT
    AAGAAACTTA CTCAAATGGA TAAAATGATC CAGGGACTTG TGAAGCGTCT GAAGAACGAC
    ACACTGCTGG TGGTGACTGG GGACCATGGG ATGACCATCA GTGGGAACCA TGGAGGAGAG
    AGTGAGCTGG AAACCTCAGC TGCACTTTTT CTATATAGTC CCAGAGCTCT CTTCCCCAGC
    GCTCCTGCAG AGGAGCCAGA GATAATTCCT CAAATCAGCC TTGTACCTAC GCTGGCCCTG
    CTGCTGGGCC TGCCCATCCC ATTTGGAAAC ATCGGGGAGG TGATAGCTGA GGTGTTCTCA
    GAGATCGAAG ACTCCCAGCC TCGCTTCTCT GCTCTGGCCC AAGCCTCAGC TCTGCATCTC
    AATGCCCAGC AGGTGTCCCG ATTTCTTCAC ACCTACTCAG CCACTGCTCA GGACCTTCAA
    GTTCAGGAGC TGCATCGATT GCAGAAGCTC TTCTCCAAGG CTTCTGCTGA CTACCAGCGA
    CTTCTGCAGA GCCCCCAGGG GGCTGAGGCA GTGCTCCAAA CTGTGATTCC TGAGCTGCAG
    CAGTTCCTGC GGGGAGTTCG GGCCATGTGC ATTGAGTCTT GGGCTCGTTT TTCTCTGGTC
    CGCATGGCAG GGGGTGCTGC TCTTCTGGCT GCTACCTGCT TTCTTTGCCT ACTGGTATCC
    CAGTGGGCAA CATCCCCAGC CTTCTATTTC GGCCCTCTCC TAAAACCCAC AGCCTCGGGT
    CTGAGTGGCG CCATGGTGTG TGCTGGACTC CTGGCATCTA CTGGGCTGAA GCTGGATTCC
    ATGGTTCTAG GGGCCATGGC TGCAGTGGGC TCACTCCTGC CTTTTCTGTG GAAAGCTTGG
    GCGAGCTGGG GATCGAAAAG GCCCCTGGCC TCTCTCCTTC CCATGCCCGG GCCAGTCCTA
    TTACTCCTGC TCATTCGCTT TGCTGGTTTC TTCTCTGATA GCTATATTAT AACCGAAGCC
    AGGGTTGCCC CCTTCCTTTT GATCTCACTC ATCTTGCTCC TGGTTGCCCG ACTTCACTGG
    GAGGGCAAGC TGCTGCCACC TAAGCTACTC TCGATACCTC GCCTTGGCTT TTTGACTCCA
    GCAGGTCCCC CGCAATACAG TGCCATGCAT GCCGTGGGAC TTGGAGTTGG GTTGCTTTTA
    TGTATAAGGC TAGCTGGACT TTTTTATCGC TGCCATGAAG AGACACCTGT TTGTAGCTCC
    TCTCCCTTGC TGAGTCCCTT GCGATCCACA GTGGATGGTC GAACCAAGAA TTTGTGGTAT
    GGAGCTTGTG TCGGGGCACT GGTGGCCCTG TTAGCCTCTG TGCGGCTGTG GCTTCACTGC
    TATGGTAATC TCAAGAGCCC TGAGCCCCCT GTGCTCTTTG TGCGCTGGGG TCTGCCCCTA
    ATGGGACTGG GCACTGCTGC CTACTGGGCA TTGGCATCAG GGGCAGATGA AGCACCCCCG
    CGTCTCCGGG CCCTGGTCTC TGGGGCATCA GTTATGTTGC CTCGGGCTGT GGCAGGGTTG
    GCCGCTTCAG GGCTCATGCT GCTGCTCTGG AGGCCTGTGA CAGTGCTGGT GAAGGCCACA
    ACAGGTGCTC CAAGGACCAG GACTGTCCTC ACTCCCTTCT CAGGTCCCCC CACTTCTCAG
    GCTGACTTGG ATTATGTGGT TCCTCAAATC TACCGACACA TACAAGAGGA GTTCCGGGGC
    CGGCTAGAGA GGACCAAATC TCAGGGTCTG CTGACGGTGG CTGCCTATCA GTTGGGGAGC
    GTCTACTCAG CTGCTGTGGT TATGGCCCTT ACCCTCTTGG CCTTCCCACT TCTGCTATTG
    CATGCAGAGC GCATTAGCCT TGTGTTCCTG CTTCTGTTTC TGCAGAGCTT CCTTCTCCTG
    CATCTGCTTG CTGCCGGGAT ACCCATCACC ACCCCTGGTC CTTTTACTGT GCCATGGCAT
    GCAGTTTTTG CATGGGCCCT CATGGCCACA CAGACCTTCT ATACCACCGG CCACCAGCCT
    GTCTTTTCAG CTATCCAATG GCATGCAGCC TTCGTGGGAG TCTCAGAGAG TCGTGACTTT
    ACCTGGCTGT CTGCTTTCCT GGTGGGAGCC AACACCTTTG CCTCCCATAT TCTCTTTGCA
    GTAGGTTGCC CGTTGCTCCT GCTCTGGCCC TTTCTGTGTG AGAATCAAGG ATCCCAGAAG
    AGGCGGCAGC CCCCAGGAAA TGAAGCTGAG GCCAGAGTCA GGCCTGAGGA GGAGCAGGAG
    CCACGGATGG AAATGCGGCT CCGGGATGCA CCTCACCACT TCAGTGCAGC ACTGCTGCAG
    CTGGGCCTCA AGTACCTCTT TGTCCTTGGT ATTCAGATTC TGGCCTGTGT CTTGGCAGCC
    TCCATCCTCC GCAGGCATCT CTTGGTCTGG AAGGTGTTTG CCCCCAAGTT CATTTTTGAG
    GCCATGGGCT TCATTGTGAG CAGCGTGGGA CTTTGTCTAG GCATAGCTTT GGTGATGCGA
    GTAGATGGTG CTGTGAGCTC CTGGTTCAGG CAGTTAGTTC TGACCCAACA GAAAGCTGGA
    GAACAATCTA GCCTGGTCTG TACGGGTGCT GGATCATCTG CAAGAAAGGC TCAGCCACAC
    CTCTTACCAC CATGCAGCCA GGACCTACTG GCATCTGGGA CTTCATTATT TTATAATTCA
    AGATCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa05813
    Clone ID Related Accession (Same CDS sequence) XM_002689674.5 , XM_024996194.1 , XM_024996195.1
    Accession Version XM_002689674.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3396bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GPI ethanolamine phosphate transferase 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002689674.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCAGAAGA TCTCAGTGCT GCTTTTCCTG GCCTGGGTGA GCTTCCTCTT CTACGCCGGC 
    ATTGCTCTCT TCACCAGTGG CTTCCTGCTC ACCCGTTGGG AGCTCACCAA CCATAGCAGC
    TGTCAAGAGC CCCCAGGCCC TGGAGCCCTG CCATGGGGGG GCCGAGGGGA ACCCGGGGCC
    TGCTGGATGG CTTCCCGATT CTCTCGGCTC GTGTTGGTGG TGATAGATGC TCTGCGATTT
    GACTTTGCCC AGCCCCAGCG CTCACCTGTT TCTGGGGAGC CTCCTGTCTC CCTGCCTTTC
    CTGGGTAAAA TGGGCTTCTT GCAGAGACTC CTGGAAAGTC AGCCCCACCA TGCCAGGCTC
    TACCAAGCCA AGGCTGACCC CCCGACCACC ACCATGCAGC GCCTCAAGGC CTTAACCACC
    GGCTCACTGC CTACCTTTGT TGATGCTGGC AGTAATTTTG CCAGCAGCGC CATAGTGGAA
    GATAATCTCA TTAAGCAGCT TTCCAGTACA GGAAGACGTG TGGTCTTCAT GGGAGATGAA
    ACCTGGAATG ATCTTTTCCC TGGAGTTTTC TCCCAAGCTT TATTCTTCCC ATCCTTCAAT
    GTCAGAGACC TAGAGACAGT GGACAATGGC ATCATGAAAC ACCTCTATCC AACCATGGAC
    AGTGACGAAT GGGACGTGCT GATTACTCAC TTCCTGGGTG TGGATCACTG TGGCCACAAG
    CATGACCCTT ACCATCCTGA AATGGCTAAG AAACTTACTC AAATGGATAA AATGATCCAG
    GGACTTGTGA AGCGTCTGAA GAACGACACA CTGCTGGTGG TGACTGGGGA CCATGGGATG
    ACCATCAGTG GGAACCATGG AGGAGAGAGT GAGCTGGAAA CCTCAGCTGC ACTTTTTCTA
    TATAGTCCCA GAGCTCTCTT CCCCAGCGCT CCTGCAGAGG AGCCAGAGAT AATTCCTCAA
    ATCAGCCTTG TACCTACGCT GGCCCTGCTG CTGGGCCTGC CCATCCCATT TGGAAACATC
    GGGGAGGTGA TAGCTGAGGT GTTCTCAGAG ATCGAAGACT CCCAGCCTCG CTTCTCTGCT
    CTGGCCCAAG CCTCAGCTCT GCATCTCAAT GCCCAGCAGG TGTCCCGATT TCTTCACACC
    TACTCAGCCA CTGCTCAGGA CCTTCAAGTT CAGGAGCTGC ATCGATTGCA GAAGCTCTTC
    TCCAAGGCTT CTGCTGACTA CCAGCGACTT CTGCAGAGCC CCCAGGGGGC TGAGGCAGTG
    CTCCAAACTG TGATTCCTGA GCTGCAGCAG TTCCTGCGGG GAGTTCGGGC CATGTGCATT
    GAGTCTTGGG CTCGTTTTTC TCTGGTCCGC ATGGCAGGGG GTGCTGCTCT TCTGGCTGCT
    ACCTGCTTTC TTTGCCTACT GGTATCCCAG TGGGCAACAT CCCCAGCCTT CTATTTCGGC
    CCTCTCCTAA AACCCACAGC CTCGGGTCTG AGTGGCGCCA TGGTGTGTGC TGGACTCCTG
    GCATCTACTG GGCTGAAGCT GGATTCCATG GTTCTAGGGG CCATGGCTGC AGTGGGCTCA
    CTCCTGCCTT TTCTGTGGAA AGCTTGGGCG AGCTGGGGAT CGAAAAGGCC CCTGGCCTCT
    CTCCTTCCCA TGCCCGGGCC AGTCCTATTA CTCCTGCTCA TTCGCTTTGC TGGTTTCTTC
    TCTGATAGCT ATATTATAAC CGAAGCCAGG GTTGCCCCCT TCCTTTTGAT CTCACTCATC
    TTGCTCCTGG TTGCCCGACT TCACTGGGAG GGCAAGCTGC TGCCACCTAA GCTACTCTCG
    ATACCTCGCC TTGGCTTTTT GACTCCAGCA GGTCCCCCGC AATACAGTGC CATGCATGCC
    GTGGGACTTG GAGTTGGGTT GCTTTTATGT ATAAGGCTAG CTGGACTTTT TTATCGCTGC
    CATGAAGAGA CACCTGTTTG TAGCTCCTCT CCCTTGCTGA GTCCCTTGCG ATCCACAGTG
    GATGGTCGAA CCAAGAATTT GTGGTATGGA GCTTGTGTCG GGGCACTGGT GGCCCTGTTA
    GCCTCTGTGC GGCTGTGGCT TCACTGCTAT GGTAATCTCA AGAGCCCTGA GCCCCCTGTG
    CTCTTTGTGC GCTGGGGTCT GCCCCTAATG GGACTGGGCA CTGCTGCCTA CTGGGCATTG
    GCATCAGGGG CAGATGAAGC ACCCCCGCGT CTCCGGGCCC TGGTCTCTGG GGCATCAGTT
    ATGTTGCCTC GGGCTGTGGC AGGGTTGGCC GCTTCAGGGC TCATGCTGCT GCTCTGGAGG
    CCTGTGACAG TGCTGGTGAA GGCCACAACA GGTGCTCCAA GGACCAGGAC TGTCCTCACT
    CCCTTCTCAG GTCCCCCCAC TTCTCAGGCT GACTTGGATT ATGTGGTTCC TCAAATCTAC
    CGACACATAC AAGAGGAGTT CCGGGGCCGG CTAGAGAGGA CCAAATCTCA GGGTCTGCTG
    ACGGTGGCTG CCTATCAGTT GGGGAGCGTC TACTCAGCTG CTGTGGTTAT GGCCCTTACC
    CTCTTGGCCT TCCCACTTCT GCTATTGCAT GCAGAGCGCA TTAGCCTTGT GTTCCTGCTT
    CTGTTTCTGC AGAGCTTCCT TCTCCTGCAT CTGCTTGCTG CCGGGATACC CATCACCACC
    CCTGGTCCTT TTACTGTGCC ATGGCATGCA GTTTTTGCAT GGGCCCTCAT GGCCACACAG
    ACCTTCTATA CCACCGGCCA CCAGCCTGTC TTTTCAGCTA TCCAATGGCA TGCAGCCTTC
    GTGGGAGTCT CAGAGAGTCG TGACTTTACC TGGCTGTCTG CTTTCCTGGT GGGAGCCAAC
    ACCTTTGCCT CCCATATTCT CTTTGCAGTA GGTTGCCCGT TGCTCCTGCT CTGGCCCTTT
    CTGTGTGAGA ATCAAGGATC CCAGAAGAGG CGGCAGCCCC CAGGAAATGA AGCTGAGGCC
    AGAGTCAGGC CTGAGGAGGA GCAGGAGCCA CGGATGGAAA TGCGGCTCCG GGATGCACCT
    CACCACTTCA GTGCAGCACT GCTGCAGCTG GGCCTCAAGT ACCTCTTTGT CCTTGGTATT
    CAGATTCTGG CCTGTGTCTT GGCAGCCTCC ATCCTCCGCA GGCATCTCTT GGTCTGGAAG
    GTGTTTGCCC CCAAGTTCAT TTTTGAGGCC ATGGGCTTCA TTGTGAGCAG CGTGGGACTT
    TGTCTAGGCA TAGCTTTGGT GATGCGAGTA GATGGTGCTG TGAGCTCCTG GTTCAGGCAG
    TTAGTTCTGA CCCAACAGAA AGCTGGAGAA CAATCTAGCC TGGTCTGTAC GGGTGCTGGA
    TCATCTGCAA GAAAGGCTCA GCCACACCTC TTACCACCAT GCAGCCAGGA CCTACTGGCA
    TCTGGGACTT CATTATTTTA TAATTCAAGA TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002689720.3
    CDS1074..4469
    Translation

    Target ORF information:

    RefSeq Version XM_002689674.5
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphatidylinositol glycan anchor biosynthesis class O (PIGO), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002689674.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCAGAAGA TCTCAGTGCT GCTTTTCCTG GCCTGGGTGA GCTTCCTCTT CTACGCCGGC 
    ATTGCTCTCT TCACCAGTGG CTTCCTGCTC ACCCGTTGGG AGCTCACCAA CCATAGCAGC
    TGTCAAGAGC CCCCAGGCCC TGGAGCCCTG CCATGGGGGG GCCGAGGGGA ACCCGGGGCC
    TGCTGGATGG CTTCCCGATT CTCTCGGCTC GTGTTGGTGG TGATAGATGC TCTGCGATTT
    GACTTTGCCC AGCCCCAGCG CTCACCTGTT TCTGGGGAGC CTCCTGTCTC CCTGCCTTTC
    CTGGGTAAAA TGGGCTTCTT GCAGAGACTC CTGGAAAGTC AGCCCCACCA TGCCAGGCTC
    TACCAAGCCA AGGCTGACCC CCCGACCACC ACCATGCAGC GCCTCAAGGC CTTAACCACC
    GGCTCACTGC CTACCTTTGT TGATGCTGGC AGTAATTTTG CCAGCAGCGC CATAGTGGAA
    GATAATCTCA TTAAGCAGCT TTCCAGTACA GGAAGACGTG TGGTCTTCAT GGGAGATGAA
    ACCTGGAATG ATCTTTTCCC TGGAGTTTTC TCCCAAGCTT TATTCTTCCC ATCCTTCAAT
    GTCAGAGACC TAGAGACAGT GGACAATGGC ATCATGAAAC ACCTCTATCC AACCATGGAC
    AGTGACGAAT GGGACGTGCT GATTACTCAC TTCCTGGGTG TGGATCACTG TGGCCACAAG
    CATGACCCTT ACCATCCTGA AATGGCTAAG AAACTTACTC AAATGGATAA AATGATCCAG
    GGACTTGTGA AGCGTCTGAA GAACGACACA CTGCTGGTGG TGACTGGGGA CCATGGGATG
    ACCATCAGTG GGAACCATGG AGGAGAGAGT GAGCTGGAAA CCTCAGCTGC ACTTTTTCTA
    TATAGTCCCA GAGCTCTCTT CCCCAGCGCT CCTGCAGAGG AGCCAGAGAT AATTCCTCAA
    ATCAGCCTTG TACCTACGCT GGCCCTGCTG CTGGGCCTGC CCATCCCATT TGGAAACATC
    GGGGAGGTGA TAGCTGAGGT GTTCTCAGAG ATCGAAGACT CCCAGCCTCG CTTCTCTGCT
    CTGGCCCAAG CCTCAGCTCT GCATCTCAAT GCCCAGCAGG TGTCCCGATT TCTTCACACC
    TACTCAGCCA CTGCTCAGGA CCTTCAAGTT CAGGAGCTGC ATCGATTGCA GAAGCTCTTC
    TCCAAGGCTT CTGCTGACTA CCAGCGACTT CTGCAGAGCC CCCAGGGGGC TGAGGCAGTG
    CTCCAAACTG TGATTCCTGA GCTGCAGCAG TTCCTGCGGG GAGTTCGGGC CATGTGCATT
    GAGTCTTGGG CTCGTTTTTC TCTGGTCCGC ATGGCAGGGG GTGCTGCTCT TCTGGCTGCT
    ACCTGCTTTC TTTGCCTACT GGTATCCCAG TGGGCAACAT CCCCAGCCTT CTATTTCGGC
    CCTCTCCTAA AACCCACAGC CTCGGGTCTG AGTGGCGCCA TGGTGTGTGC TGGACTCCTG
    GCATCTACTG GGCTGAAGCT GGATTCCATG GTTCTAGGGG CCATGGCTGC AGTGGGCTCA
    CTCCTGCCTT TTCTGTGGAA AGCTTGGGCG AGCTGGGGAT CGAAAAGGCC CCTGGCCTCT
    CTCCTTCCCA TGCCCGGGCC AGTCCTATTA CTCCTGCTCA TTCGCTTTGC TGGTTTCTTC
    TCTGATAGCT ATATTATAAC CGAAGCCAGG GTTGCCCCCT TCCTTTTGAT CTCACTCATC
    TTGCTCCTGG TTGCCCGACT TCACTGGGAG GGCAAGCTGC TGCCACCTAA GCTACTCTCG
    ATACCTCGCC TTGGCTTTTT GACTCCAGCA GGTCCCCCGC AATACAGTGC CATGCATGCC
    GTGGGACTTG GAGTTGGGTT GCTTTTATGT ATAAGGCTAG CTGGACTTTT TTATCGCTGC
    CATGAAGAGA CACCTGTTTG TAGCTCCTCT CCCTTGCTGA GTCCCTTGCG ATCCACAGTG
    GATGGTCGAA CCAAGAATTT GTGGTATGGA GCTTGTGTCG GGGCACTGGT GGCCCTGTTA
    GCCTCTGTGC GGCTGTGGCT TCACTGCTAT GGTAATCTCA AGAGCCCTGA GCCCCCTGTG
    CTCTTTGTGC GCTGGGGTCT GCCCCTAATG GGACTGGGCA CTGCTGCCTA CTGGGCATTG
    GCATCAGGGG CAGATGAAGC ACCCCCGCGT CTCCGGGCCC TGGTCTCTGG GGCATCAGTT
    ATGTTGCCTC GGGCTGTGGC AGGGTTGGCC GCTTCAGGGC TCATGCTGCT GCTCTGGAGG
    CCTGTGACAG TGCTGGTGAA GGCCACAACA GGTGCTCCAA GGACCAGGAC TGTCCTCACT
    CCCTTCTCAG GTCCCCCCAC TTCTCAGGCT GACTTGGATT ATGTGGTTCC TCAAATCTAC
    CGACACATAC AAGAGGAGTT CCGGGGCCGG CTAGAGAGGA CCAAATCTCA GGGTCTGCTG
    ACGGTGGCTG CCTATCAGTT GGGGAGCGTC TACTCAGCTG CTGTGGTTAT GGCCCTTACC
    CTCTTGGCCT TCCCACTTCT GCTATTGCAT GCAGAGCGCA TTAGCCTTGT GTTCCTGCTT
    CTGTTTCTGC AGAGCTTCCT TCTCCTGCAT CTGCTTGCTG CCGGGATACC CATCACCACC
    CCTGGTCCTT TTACTGTGCC ATGGCATGCA GTTTTTGCAT GGGCCCTCAT GGCCACACAG
    ACCTTCTATA CCACCGGCCA CCAGCCTGTC TTTTCAGCTA TCCAATGGCA TGCAGCCTTC
    GTGGGAGTCT CAGAGAGTCG TGACTTTACC TGGCTGTCTG CTTTCCTGGT GGGAGCCAAC
    ACCTTTGCCT CCCATATTCT CTTTGCAGTA GGTTGCCCGT TGCTCCTGCT CTGGCCCTTT
    CTGTGTGAGA ATCAAGGATC CCAGAAGAGG CGGCAGCCCC CAGGAAATGA AGCTGAGGCC
    AGAGTCAGGC CTGAGGAGGA GCAGGAGCCA CGGATGGAAA TGCGGCTCCG GGATGCACCT
    CACCACTTCA GTGCAGCACT GCTGCAGCTG GGCCTCAAGT ACCTCTTTGT CCTTGGTATT
    CAGATTCTGG CCTGTGTCTT GGCAGCCTCC ATCCTCCGCA GGCATCTCTT GGTCTGGAAG
    GTGTTTGCCC CCAAGTTCAT TTTTGAGGCC ATGGGCTTCA TTGTGAGCAG CGTGGGACTT
    TGTCTAGGCA TAGCTTTGGT GATGCGAGTA GATGGTGCTG TGAGCTCCTG GTTCAGGCAG
    TTAGTTCTGA CCCAACAGAA AGCTGGAGAA CAATCTAGCC TGGTCTGTAC GGGTGCTGGA
    TCATCTGCAA GAAAGGCTCA GCCACACCTC TTACCACCAT GCAGCCAGGA CCTACTGGCA
    TCTGGGACTT CATTATTTTA TAATTCAAGA TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa05813
    Clone ID Related Accession (Same CDS sequence) XM_002689674.5 , XM_024996194.1 , XM_024996195.1
    Accession Version XM_024996194.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3396bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GPI ethanolamine phosphate transferase 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCAGAAGA TCTCAGTGCT GCTTTTCCTG GCCTGGGTGA GCTTCCTCTT CTACGCCGGC 
    ATTGCTCTCT TCACCAGTGG CTTCCTGCTC ACCCGTTGGG AGCTCACCAA CCATAGCAGC
    TGTCAAGAGC CCCCAGGCCC TGGAGCCCTG CCATGGGGGG GCCGAGGGGA ACCCGGGGCC
    TGCTGGATGG CTTCCCGATT CTCTCGGCTC GTGTTGGTGG TGATAGATGC TCTGCGATTT
    GACTTTGCCC AGCCCCAGCG CTCACCTGTT TCTGGGGAGC CTCCTGTCTC CCTGCCTTTC
    CTGGGTAAAA TGGGCTTCTT GCAGAGACTC CTGGAAAGTC AGCCCCACCA TGCCAGGCTC
    TACCAAGCCA AGGCTGACCC CCCGACCACC ACCATGCAGC GCCTCAAGGC CTTAACCACC
    GGCTCACTGC CTACCTTTGT TGATGCTGGC AGTAATTTTG CCAGCAGCGC CATAGTGGAA
    GATAATCTCA TTAAGCAGCT TTCCAGTACA GGAAGACGTG TGGTCTTCAT GGGAGATGAA
    ACCTGGAATG ATCTTTTCCC TGGAGTTTTC TCCCAAGCTT TATTCTTCCC ATCCTTCAAT
    GTCAGAGACC TAGAGACAGT GGACAATGGC ATCATGAAAC ACCTCTATCC AACCATGGAC
    AGTGACGAAT GGGACGTGCT GATTACTCAC TTCCTGGGTG TGGATCACTG TGGCCACAAG
    CATGACCCTT ACCATCCTGA AATGGCTAAG AAACTTACTC AAATGGATAA AATGATCCAG
    GGACTTGTGA AGCGTCTGAA GAACGACACA CTGCTGGTGG TGACTGGGGA CCATGGGATG
    ACCATCAGTG GGAACCATGG AGGAGAGAGT GAGCTGGAAA CCTCAGCTGC ACTTTTTCTA
    TATAGTCCCA GAGCTCTCTT CCCCAGCGCT CCTGCAGAGG AGCCAGAGAT AATTCCTCAA
    ATCAGCCTTG TACCTACGCT GGCCCTGCTG CTGGGCCTGC CCATCCCATT TGGAAACATC
    GGGGAGGTGA TAGCTGAGGT GTTCTCAGAG ATCGAAGACT CCCAGCCTCG CTTCTCTGCT
    CTGGCCCAAG CCTCAGCTCT GCATCTCAAT GCCCAGCAGG TGTCCCGATT TCTTCACACC
    TACTCAGCCA CTGCTCAGGA CCTTCAAGTT CAGGAGCTGC ATCGATTGCA GAAGCTCTTC
    TCCAAGGCTT CTGCTGACTA CCAGCGACTT CTGCAGAGCC CCCAGGGGGC TGAGGCAGTG
    CTCCAAACTG TGATTCCTGA GCTGCAGCAG TTCCTGCGGG GAGTTCGGGC CATGTGCATT
    GAGTCTTGGG CTCGTTTTTC TCTGGTCCGC ATGGCAGGGG GTGCTGCTCT TCTGGCTGCT
    ACCTGCTTTC TTTGCCTACT GGTATCCCAG TGGGCAACAT CCCCAGCCTT CTATTTCGGC
    CCTCTCCTAA AACCCACAGC CTCGGGTCTG AGTGGCGCCA TGGTGTGTGC TGGACTCCTG
    GCATCTACTG GGCTGAAGCT GGATTCCATG GTTCTAGGGG CCATGGCTGC AGTGGGCTCA
    CTCCTGCCTT TTCTGTGGAA AGCTTGGGCG AGCTGGGGAT CGAAAAGGCC CCTGGCCTCT
    CTCCTTCCCA TGCCCGGGCC AGTCCTATTA CTCCTGCTCA TTCGCTTTGC TGGTTTCTTC
    TCTGATAGCT ATATTATAAC CGAAGCCAGG GTTGCCCCCT TCCTTTTGAT CTCACTCATC
    TTGCTCCTGG TTGCCCGACT TCACTGGGAG GGCAAGCTGC TGCCACCTAA GCTACTCTCG
    ATACCTCGCC TTGGCTTTTT GACTCCAGCA GGTCCCCCGC AATACAGTGC CATGCATGCC
    GTGGGACTTG GAGTTGGGTT GCTTTTATGT ATAAGGCTAG CTGGACTTTT TTATCGCTGC
    CATGAAGAGA CACCTGTTTG TAGCTCCTCT CCCTTGCTGA GTCCCTTGCG ATCCACAGTG
    GATGGTCGAA CCAAGAATTT GTGGTATGGA GCTTGTGTCG GGGCACTGGT GGCCCTGTTA
    GCCTCTGTGC GGCTGTGGCT TCACTGCTAT GGTAATCTCA AGAGCCCTGA GCCCCCTGTG
    CTCTTTGTGC GCTGGGGTCT GCCCCTAATG GGACTGGGCA CTGCTGCCTA CTGGGCATTG
    GCATCAGGGG CAGATGAAGC ACCCCCGCGT CTCCGGGCCC TGGTCTCTGG GGCATCAGTT
    ATGTTGCCTC GGGCTGTGGC AGGGTTGGCC GCTTCAGGGC TCATGCTGCT GCTCTGGAGG
    CCTGTGACAG TGCTGGTGAA GGCCACAACA GGTGCTCCAA GGACCAGGAC TGTCCTCACT
    CCCTTCTCAG GTCCCCCCAC TTCTCAGGCT GACTTGGATT ATGTGGTTCC TCAAATCTAC
    CGACACATAC AAGAGGAGTT CCGGGGCCGG CTAGAGAGGA CCAAATCTCA GGGTCTGCTG
    ACGGTGGCTG CCTATCAGTT GGGGAGCGTC TACTCAGCTG CTGTGGTTAT GGCCCTTACC
    CTCTTGGCCT TCCCACTTCT GCTATTGCAT GCAGAGCGCA TTAGCCTTGT GTTCCTGCTT
    CTGTTTCTGC AGAGCTTCCT TCTCCTGCAT CTGCTTGCTG CCGGGATACC CATCACCACC
    CCTGGTCCTT TTACTGTGCC ATGGCATGCA GTTTTTGCAT GGGCCCTCAT GGCCACACAG
    ACCTTCTATA CCACCGGCCA CCAGCCTGTC TTTTCAGCTA TCCAATGGCA TGCAGCCTTC
    GTGGGAGTCT CAGAGAGTCG TGACTTTACC TGGCTGTCTG CTTTCCTGGT GGGAGCCAAC
    ACCTTTGCCT CCCATATTCT CTTTGCAGTA GGTTGCCCGT TGCTCCTGCT CTGGCCCTTT
    CTGTGTGAGA ATCAAGGATC CCAGAAGAGG CGGCAGCCCC CAGGAAATGA AGCTGAGGCC
    AGAGTCAGGC CTGAGGAGGA GCAGGAGCCA CGGATGGAAA TGCGGCTCCG GGATGCACCT
    CACCACTTCA GTGCAGCACT GCTGCAGCTG GGCCTCAAGT ACCTCTTTGT CCTTGGTATT
    CAGATTCTGG CCTGTGTCTT GGCAGCCTCC ATCCTCCGCA GGCATCTCTT GGTCTGGAAG
    GTGTTTGCCC CCAAGTTCAT TTTTGAGGCC ATGGGCTTCA TTGTGAGCAG CGTGGGACTT
    TGTCTAGGCA TAGCTTTGGT GATGCGAGTA GATGGTGCTG TGAGCTCCTG GTTCAGGCAG
    TTAGTTCTGA CCCAACAGAA AGCTGGAGAA CAATCTAGCC TGGTCTGTAC GGGTGCTGGA
    TCATCTGCAA GAAAGGCTCA GCCACACCTC TTACCACCAT GCAGCCAGGA CCTACTGGCA
    TCTGGGACTT CATTATTTTA TAATTCAAGA TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851962.1
    CDS592..3987
    Translation

    Target ORF information:

    RefSeq Version XM_024996194.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphatidylinositol glycan anchor biosynthesis class O (PIGO), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996194.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCAGAAGA TCTCAGTGCT GCTTTTCCTG GCCTGGGTGA GCTTCCTCTT CTACGCCGGC 
    ATTGCTCTCT TCACCAGTGG CTTCCTGCTC ACCCGTTGGG AGCTCACCAA CCATAGCAGC
    TGTCAAGAGC CCCCAGGCCC TGGAGCCCTG CCATGGGGGG GCCGAGGGGA ACCCGGGGCC
    TGCTGGATGG CTTCCCGATT CTCTCGGCTC GTGTTGGTGG TGATAGATGC TCTGCGATTT
    GACTTTGCCC AGCCCCAGCG CTCACCTGTT TCTGGGGAGC CTCCTGTCTC CCTGCCTTTC
    CTGGGTAAAA TGGGCTTCTT GCAGAGACTC CTGGAAAGTC AGCCCCACCA TGCCAGGCTC
    TACCAAGCCA AGGCTGACCC CCCGACCACC ACCATGCAGC GCCTCAAGGC CTTAACCACC
    GGCTCACTGC CTACCTTTGT TGATGCTGGC AGTAATTTTG CCAGCAGCGC CATAGTGGAA
    GATAATCTCA TTAAGCAGCT TTCCAGTACA GGAAGACGTG TGGTCTTCAT GGGAGATGAA
    ACCTGGAATG ATCTTTTCCC TGGAGTTTTC TCCCAAGCTT TATTCTTCCC ATCCTTCAAT
    GTCAGAGACC TAGAGACAGT GGACAATGGC ATCATGAAAC ACCTCTATCC AACCATGGAC
    AGTGACGAAT GGGACGTGCT GATTACTCAC TTCCTGGGTG TGGATCACTG TGGCCACAAG
    CATGACCCTT ACCATCCTGA AATGGCTAAG AAACTTACTC AAATGGATAA AATGATCCAG
    GGACTTGTGA AGCGTCTGAA GAACGACACA CTGCTGGTGG TGACTGGGGA CCATGGGATG
    ACCATCAGTG GGAACCATGG AGGAGAGAGT GAGCTGGAAA CCTCAGCTGC ACTTTTTCTA
    TATAGTCCCA GAGCTCTCTT CCCCAGCGCT CCTGCAGAGG AGCCAGAGAT AATTCCTCAA
    ATCAGCCTTG TACCTACGCT GGCCCTGCTG CTGGGCCTGC CCATCCCATT TGGAAACATC
    GGGGAGGTGA TAGCTGAGGT GTTCTCAGAG ATCGAAGACT CCCAGCCTCG CTTCTCTGCT
    CTGGCCCAAG CCTCAGCTCT GCATCTCAAT GCCCAGCAGG TGTCCCGATT TCTTCACACC
    TACTCAGCCA CTGCTCAGGA CCTTCAAGTT CAGGAGCTGC ATCGATTGCA GAAGCTCTTC
    TCCAAGGCTT CTGCTGACTA CCAGCGACTT CTGCAGAGCC CCCAGGGGGC TGAGGCAGTG
    CTCCAAACTG TGATTCCTGA GCTGCAGCAG TTCCTGCGGG GAGTTCGGGC CATGTGCATT
    GAGTCTTGGG CTCGTTTTTC TCTGGTCCGC ATGGCAGGGG GTGCTGCTCT TCTGGCTGCT
    ACCTGCTTTC TTTGCCTACT GGTATCCCAG TGGGCAACAT CCCCAGCCTT CTATTTCGGC
    CCTCTCCTAA AACCCACAGC CTCGGGTCTG AGTGGCGCCA TGGTGTGTGC TGGACTCCTG
    GCATCTACTG GGCTGAAGCT GGATTCCATG GTTCTAGGGG CCATGGCTGC AGTGGGCTCA
    CTCCTGCCTT TTCTGTGGAA AGCTTGGGCG AGCTGGGGAT CGAAAAGGCC CCTGGCCTCT
    CTCCTTCCCA TGCCCGGGCC AGTCCTATTA CTCCTGCTCA TTCGCTTTGC TGGTTTCTTC
    TCTGATAGCT ATATTATAAC CGAAGCCAGG GTTGCCCCCT TCCTTTTGAT CTCACTCATC
    TTGCTCCTGG TTGCCCGACT TCACTGGGAG GGCAAGCTGC TGCCACCTAA GCTACTCTCG
    ATACCTCGCC TTGGCTTTTT GACTCCAGCA GGTCCCCCGC AATACAGTGC CATGCATGCC
    GTGGGACTTG GAGTTGGGTT GCTTTTATGT ATAAGGCTAG CTGGACTTTT TTATCGCTGC
    CATGAAGAGA CACCTGTTTG TAGCTCCTCT CCCTTGCTGA GTCCCTTGCG ATCCACAGTG
    GATGGTCGAA CCAAGAATTT GTGGTATGGA GCTTGTGTCG GGGCACTGGT GGCCCTGTTA
    GCCTCTGTGC GGCTGTGGCT TCACTGCTAT GGTAATCTCA AGAGCCCTGA GCCCCCTGTG
    CTCTTTGTGC GCTGGGGTCT GCCCCTAATG GGACTGGGCA CTGCTGCCTA CTGGGCATTG
    GCATCAGGGG CAGATGAAGC ACCCCCGCGT CTCCGGGCCC TGGTCTCTGG GGCATCAGTT
    ATGTTGCCTC GGGCTGTGGC AGGGTTGGCC GCTTCAGGGC TCATGCTGCT GCTCTGGAGG
    CCTGTGACAG TGCTGGTGAA GGCCACAACA GGTGCTCCAA GGACCAGGAC TGTCCTCACT
    CCCTTCTCAG GTCCCCCCAC TTCTCAGGCT GACTTGGATT ATGTGGTTCC TCAAATCTAC
    CGACACATAC AAGAGGAGTT CCGGGGCCGG CTAGAGAGGA CCAAATCTCA GGGTCTGCTG
    ACGGTGGCTG CCTATCAGTT GGGGAGCGTC TACTCAGCTG CTGTGGTTAT GGCCCTTACC
    CTCTTGGCCT TCCCACTTCT GCTATTGCAT GCAGAGCGCA TTAGCCTTGT GTTCCTGCTT
    CTGTTTCTGC AGAGCTTCCT TCTCCTGCAT CTGCTTGCTG CCGGGATACC CATCACCACC
    CCTGGTCCTT TTACTGTGCC ATGGCATGCA GTTTTTGCAT GGGCCCTCAT GGCCACACAG
    ACCTTCTATA CCACCGGCCA CCAGCCTGTC TTTTCAGCTA TCCAATGGCA TGCAGCCTTC
    GTGGGAGTCT CAGAGAGTCG TGACTTTACC TGGCTGTCTG CTTTCCTGGT GGGAGCCAAC
    ACCTTTGCCT CCCATATTCT CTTTGCAGTA GGTTGCCCGT TGCTCCTGCT CTGGCCCTTT
    CTGTGTGAGA ATCAAGGATC CCAGAAGAGG CGGCAGCCCC CAGGAAATGA AGCTGAGGCC
    AGAGTCAGGC CTGAGGAGGA GCAGGAGCCA CGGATGGAAA TGCGGCTCCG GGATGCACCT
    CACCACTTCA GTGCAGCACT GCTGCAGCTG GGCCTCAAGT ACCTCTTTGT CCTTGGTATT
    CAGATTCTGG CCTGTGTCTT GGCAGCCTCC ATCCTCCGCA GGCATCTCTT GGTCTGGAAG
    GTGTTTGCCC CCAAGTTCAT TTTTGAGGCC ATGGGCTTCA TTGTGAGCAG CGTGGGACTT
    TGTCTAGGCA TAGCTTTGGT GATGCGAGTA GATGGTGCTG TGAGCTCCTG GTTCAGGCAG
    TTAGTTCTGA CCCAACAGAA AGCTGGAGAA CAATCTAGCC TGGTCTGTAC GGGTGCTGGA
    TCATCTGCAA GAAAGGCTCA GCCACACCTC TTACCACCAT GCAGCCAGGA CCTACTGGCA
    TCTGGGACTT CATTATTTTA TAATTCAAGA TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa05813
    Clone ID Related Accession (Same CDS sequence) XM_002689674.5 , XM_024996194.1 , XM_024996195.1
    Accession Version XM_024996195.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3396bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product GPI ethanolamine phosphate transferase 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCAGAAGA TCTCAGTGCT GCTTTTCCTG GCCTGGGTGA GCTTCCTCTT CTACGCCGGC 
    ATTGCTCTCT TCACCAGTGG CTTCCTGCTC ACCCGTTGGG AGCTCACCAA CCATAGCAGC
    TGTCAAGAGC CCCCAGGCCC TGGAGCCCTG CCATGGGGGG GCCGAGGGGA ACCCGGGGCC
    TGCTGGATGG CTTCCCGATT CTCTCGGCTC GTGTTGGTGG TGATAGATGC TCTGCGATTT
    GACTTTGCCC AGCCCCAGCG CTCACCTGTT TCTGGGGAGC CTCCTGTCTC CCTGCCTTTC
    CTGGGTAAAA TGGGCTTCTT GCAGAGACTC CTGGAAAGTC AGCCCCACCA TGCCAGGCTC
    TACCAAGCCA AGGCTGACCC CCCGACCACC ACCATGCAGC GCCTCAAGGC CTTAACCACC
    GGCTCACTGC CTACCTTTGT TGATGCTGGC AGTAATTTTG CCAGCAGCGC CATAGTGGAA
    GATAATCTCA TTAAGCAGCT TTCCAGTACA GGAAGACGTG TGGTCTTCAT GGGAGATGAA
    ACCTGGAATG ATCTTTTCCC TGGAGTTTTC TCCCAAGCTT TATTCTTCCC ATCCTTCAAT
    GTCAGAGACC TAGAGACAGT GGACAATGGC ATCATGAAAC ACCTCTATCC AACCATGGAC
    AGTGACGAAT GGGACGTGCT GATTACTCAC TTCCTGGGTG TGGATCACTG TGGCCACAAG
    CATGACCCTT ACCATCCTGA AATGGCTAAG AAACTTACTC AAATGGATAA AATGATCCAG
    GGACTTGTGA AGCGTCTGAA GAACGACACA CTGCTGGTGG TGACTGGGGA CCATGGGATG
    ACCATCAGTG GGAACCATGG AGGAGAGAGT GAGCTGGAAA CCTCAGCTGC ACTTTTTCTA
    TATAGTCCCA GAGCTCTCTT CCCCAGCGCT CCTGCAGAGG AGCCAGAGAT AATTCCTCAA
    ATCAGCCTTG TACCTACGCT GGCCCTGCTG CTGGGCCTGC CCATCCCATT TGGAAACATC
    GGGGAGGTGA TAGCTGAGGT GTTCTCAGAG ATCGAAGACT CCCAGCCTCG CTTCTCTGCT
    CTGGCCCAAG CCTCAGCTCT GCATCTCAAT GCCCAGCAGG TGTCCCGATT TCTTCACACC
    TACTCAGCCA CTGCTCAGGA CCTTCAAGTT CAGGAGCTGC ATCGATTGCA GAAGCTCTTC
    TCCAAGGCTT CTGCTGACTA CCAGCGACTT CTGCAGAGCC CCCAGGGGGC TGAGGCAGTG
    CTCCAAACTG TGATTCCTGA GCTGCAGCAG TTCCTGCGGG GAGTTCGGGC CATGTGCATT
    GAGTCTTGGG CTCGTTTTTC TCTGGTCCGC ATGGCAGGGG GTGCTGCTCT TCTGGCTGCT
    ACCTGCTTTC TTTGCCTACT GGTATCCCAG TGGGCAACAT CCCCAGCCTT CTATTTCGGC
    CCTCTCCTAA AACCCACAGC CTCGGGTCTG AGTGGCGCCA TGGTGTGTGC TGGACTCCTG
    GCATCTACTG GGCTGAAGCT GGATTCCATG GTTCTAGGGG CCATGGCTGC AGTGGGCTCA
    CTCCTGCCTT TTCTGTGGAA AGCTTGGGCG AGCTGGGGAT CGAAAAGGCC CCTGGCCTCT
    CTCCTTCCCA TGCCCGGGCC AGTCCTATTA CTCCTGCTCA TTCGCTTTGC TGGTTTCTTC
    TCTGATAGCT ATATTATAAC CGAAGCCAGG GTTGCCCCCT TCCTTTTGAT CTCACTCATC
    TTGCTCCTGG TTGCCCGACT TCACTGGGAG GGCAAGCTGC TGCCACCTAA GCTACTCTCG
    ATACCTCGCC TTGGCTTTTT GACTCCAGCA GGTCCCCCGC AATACAGTGC CATGCATGCC
    GTGGGACTTG GAGTTGGGTT GCTTTTATGT ATAAGGCTAG CTGGACTTTT TTATCGCTGC
    CATGAAGAGA CACCTGTTTG TAGCTCCTCT CCCTTGCTGA GTCCCTTGCG ATCCACAGTG
    GATGGTCGAA CCAAGAATTT GTGGTATGGA GCTTGTGTCG GGGCACTGGT GGCCCTGTTA
    GCCTCTGTGC GGCTGTGGCT TCACTGCTAT GGTAATCTCA AGAGCCCTGA GCCCCCTGTG
    CTCTTTGTGC GCTGGGGTCT GCCCCTAATG GGACTGGGCA CTGCTGCCTA CTGGGCATTG
    GCATCAGGGG CAGATGAAGC ACCCCCGCGT CTCCGGGCCC TGGTCTCTGG GGCATCAGTT
    ATGTTGCCTC GGGCTGTGGC AGGGTTGGCC GCTTCAGGGC TCATGCTGCT GCTCTGGAGG
    CCTGTGACAG TGCTGGTGAA GGCCACAACA GGTGCTCCAA GGACCAGGAC TGTCCTCACT
    CCCTTCTCAG GTCCCCCCAC TTCTCAGGCT GACTTGGATT ATGTGGTTCC TCAAATCTAC
    CGACACATAC AAGAGGAGTT CCGGGGCCGG CTAGAGAGGA CCAAATCTCA GGGTCTGCTG
    ACGGTGGCTG CCTATCAGTT GGGGAGCGTC TACTCAGCTG CTGTGGTTAT GGCCCTTACC
    CTCTTGGCCT TCCCACTTCT GCTATTGCAT GCAGAGCGCA TTAGCCTTGT GTTCCTGCTT
    CTGTTTCTGC AGAGCTTCCT TCTCCTGCAT CTGCTTGCTG CCGGGATACC CATCACCACC
    CCTGGTCCTT TTACTGTGCC ATGGCATGCA GTTTTTGCAT GGGCCCTCAT GGCCACACAG
    ACCTTCTATA CCACCGGCCA CCAGCCTGTC TTTTCAGCTA TCCAATGGCA TGCAGCCTTC
    GTGGGAGTCT CAGAGAGTCG TGACTTTACC TGGCTGTCTG CTTTCCTGGT GGGAGCCAAC
    ACCTTTGCCT CCCATATTCT CTTTGCAGTA GGTTGCCCGT TGCTCCTGCT CTGGCCCTTT
    CTGTGTGAGA ATCAAGGATC CCAGAAGAGG CGGCAGCCCC CAGGAAATGA AGCTGAGGCC
    AGAGTCAGGC CTGAGGAGGA GCAGGAGCCA CGGATGGAAA TGCGGCTCCG GGATGCACCT
    CACCACTTCA GTGCAGCACT GCTGCAGCTG GGCCTCAAGT ACCTCTTTGT CCTTGGTATT
    CAGATTCTGG CCTGTGTCTT GGCAGCCTCC ATCCTCCGCA GGCATCTCTT GGTCTGGAAG
    GTGTTTGCCC CCAAGTTCAT TTTTGAGGCC ATGGGCTTCA TTGTGAGCAG CGTGGGACTT
    TGTCTAGGCA TAGCTTTGGT GATGCGAGTA GATGGTGCTG TGAGCTCCTG GTTCAGGCAG
    TTAGTTCTGA CCCAACAGAA AGCTGGAGAA CAATCTAGCC TGGTCTGTAC GGGTGCTGGA
    TCATCTGCAA GAAAGGCTCA GCCACACCTC TTACCACCAT GCAGCCAGGA CCTACTGGCA
    TCTGGGACTT CATTATTTTA TAATTCAAGA TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851963.1
    CDS157..3552
    Translation

    Target ORF information:

    RefSeq Version XM_024996195.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphatidylinositol glycan anchor biosynthesis class O (PIGO), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996195.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCAGAAGA TCTCAGTGCT GCTTTTCCTG GCCTGGGTGA GCTTCCTCTT CTACGCCGGC 
    ATTGCTCTCT TCACCAGTGG CTTCCTGCTC ACCCGTTGGG AGCTCACCAA CCATAGCAGC
    TGTCAAGAGC CCCCAGGCCC TGGAGCCCTG CCATGGGGGG GCCGAGGGGA ACCCGGGGCC
    TGCTGGATGG CTTCCCGATT CTCTCGGCTC GTGTTGGTGG TGATAGATGC TCTGCGATTT
    GACTTTGCCC AGCCCCAGCG CTCACCTGTT TCTGGGGAGC CTCCTGTCTC CCTGCCTTTC
    CTGGGTAAAA TGGGCTTCTT GCAGAGACTC CTGGAAAGTC AGCCCCACCA TGCCAGGCTC
    TACCAAGCCA AGGCTGACCC CCCGACCACC ACCATGCAGC GCCTCAAGGC CTTAACCACC
    GGCTCACTGC CTACCTTTGT TGATGCTGGC AGTAATTTTG CCAGCAGCGC CATAGTGGAA
    GATAATCTCA TTAAGCAGCT TTCCAGTACA GGAAGACGTG TGGTCTTCAT GGGAGATGAA
    ACCTGGAATG ATCTTTTCCC TGGAGTTTTC TCCCAAGCTT TATTCTTCCC ATCCTTCAAT
    GTCAGAGACC TAGAGACAGT GGACAATGGC ATCATGAAAC ACCTCTATCC AACCATGGAC
    AGTGACGAAT GGGACGTGCT GATTACTCAC TTCCTGGGTG TGGATCACTG TGGCCACAAG
    CATGACCCTT ACCATCCTGA AATGGCTAAG AAACTTACTC AAATGGATAA AATGATCCAG
    GGACTTGTGA AGCGTCTGAA GAACGACACA CTGCTGGTGG TGACTGGGGA CCATGGGATG
    ACCATCAGTG GGAACCATGG AGGAGAGAGT GAGCTGGAAA CCTCAGCTGC ACTTTTTCTA
    TATAGTCCCA GAGCTCTCTT CCCCAGCGCT CCTGCAGAGG AGCCAGAGAT AATTCCTCAA
    ATCAGCCTTG TACCTACGCT GGCCCTGCTG CTGGGCCTGC CCATCCCATT TGGAAACATC
    GGGGAGGTGA TAGCTGAGGT GTTCTCAGAG ATCGAAGACT CCCAGCCTCG CTTCTCTGCT
    CTGGCCCAAG CCTCAGCTCT GCATCTCAAT GCCCAGCAGG TGTCCCGATT TCTTCACACC
    TACTCAGCCA CTGCTCAGGA CCTTCAAGTT CAGGAGCTGC ATCGATTGCA GAAGCTCTTC
    TCCAAGGCTT CTGCTGACTA CCAGCGACTT CTGCAGAGCC CCCAGGGGGC TGAGGCAGTG
    CTCCAAACTG TGATTCCTGA GCTGCAGCAG TTCCTGCGGG GAGTTCGGGC CATGTGCATT
    GAGTCTTGGG CTCGTTTTTC TCTGGTCCGC ATGGCAGGGG GTGCTGCTCT TCTGGCTGCT
    ACCTGCTTTC TTTGCCTACT GGTATCCCAG TGGGCAACAT CCCCAGCCTT CTATTTCGGC
    CCTCTCCTAA AACCCACAGC CTCGGGTCTG AGTGGCGCCA TGGTGTGTGC TGGACTCCTG
    GCATCTACTG GGCTGAAGCT GGATTCCATG GTTCTAGGGG CCATGGCTGC AGTGGGCTCA
    CTCCTGCCTT TTCTGTGGAA AGCTTGGGCG AGCTGGGGAT CGAAAAGGCC CCTGGCCTCT
    CTCCTTCCCA TGCCCGGGCC AGTCCTATTA CTCCTGCTCA TTCGCTTTGC TGGTTTCTTC
    TCTGATAGCT ATATTATAAC CGAAGCCAGG GTTGCCCCCT TCCTTTTGAT CTCACTCATC
    TTGCTCCTGG TTGCCCGACT TCACTGGGAG GGCAAGCTGC TGCCACCTAA GCTACTCTCG
    ATACCTCGCC TTGGCTTTTT GACTCCAGCA GGTCCCCCGC AATACAGTGC CATGCATGCC
    GTGGGACTTG GAGTTGGGTT GCTTTTATGT ATAAGGCTAG CTGGACTTTT TTATCGCTGC
    CATGAAGAGA CACCTGTTTG TAGCTCCTCT CCCTTGCTGA GTCCCTTGCG ATCCACAGTG
    GATGGTCGAA CCAAGAATTT GTGGTATGGA GCTTGTGTCG GGGCACTGGT GGCCCTGTTA
    GCCTCTGTGC GGCTGTGGCT TCACTGCTAT GGTAATCTCA AGAGCCCTGA GCCCCCTGTG
    CTCTTTGTGC GCTGGGGTCT GCCCCTAATG GGACTGGGCA CTGCTGCCTA CTGGGCATTG
    GCATCAGGGG CAGATGAAGC ACCCCCGCGT CTCCGGGCCC TGGTCTCTGG GGCATCAGTT
    ATGTTGCCTC GGGCTGTGGC AGGGTTGGCC GCTTCAGGGC TCATGCTGCT GCTCTGGAGG
    CCTGTGACAG TGCTGGTGAA GGCCACAACA GGTGCTCCAA GGACCAGGAC TGTCCTCACT
    CCCTTCTCAG GTCCCCCCAC TTCTCAGGCT GACTTGGATT ATGTGGTTCC TCAAATCTAC
    CGACACATAC AAGAGGAGTT CCGGGGCCGG CTAGAGAGGA CCAAATCTCA GGGTCTGCTG
    ACGGTGGCTG CCTATCAGTT GGGGAGCGTC TACTCAGCTG CTGTGGTTAT GGCCCTTACC
    CTCTTGGCCT TCCCACTTCT GCTATTGCAT GCAGAGCGCA TTAGCCTTGT GTTCCTGCTT
    CTGTTTCTGC AGAGCTTCCT TCTCCTGCAT CTGCTTGCTG CCGGGATACC CATCACCACC
    CCTGGTCCTT TTACTGTGCC ATGGCATGCA GTTTTTGCAT GGGCCCTCAT GGCCACACAG
    ACCTTCTATA CCACCGGCCA CCAGCCTGTC TTTTCAGCTA TCCAATGGCA TGCAGCCTTC
    GTGGGAGTCT CAGAGAGTCG TGACTTTACC TGGCTGTCTG CTTTCCTGGT GGGAGCCAAC
    ACCTTTGCCT CCCATATTCT CTTTGCAGTA GGTTGCCCGT TGCTCCTGCT CTGGCCCTTT
    CTGTGTGAGA ATCAAGGATC CCAGAAGAGG CGGCAGCCCC CAGGAAATGA AGCTGAGGCC
    AGAGTCAGGC CTGAGGAGGA GCAGGAGCCA CGGATGGAAA TGCGGCTCCG GGATGCACCT
    CACCACTTCA GTGCAGCACT GCTGCAGCTG GGCCTCAAGT ACCTCTTTGT CCTTGGTATT
    CAGATTCTGG CCTGTGTCTT GGCAGCCTCC ATCCTCCGCA GGCATCTCTT GGTCTGGAAG
    GTGTTTGCCC CCAAGTTCAT TTTTGAGGCC ATGGGCTTCA TTGTGAGCAG CGTGGGACTT
    TGTCTAGGCA TAGCTTTGGT GATGCGAGTA GATGGTGCTG TGAGCTCCTG GTTCAGGCAG
    TTAGTTCTGA CCCAACAGAA AGCTGGAGAA CAATCTAGCC TGGTCTGTAC GGGTGCTGGA
    TCATCTGCAA GAAAGGCTCA GCCACACCTC TTACCACCAT GCAGCCAGGA CCTACTGGCA
    TCTGGGACTT CATTATTTTA TAATTCAAGA TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91283
    Clone ID Related Accession (Same CDS sequence) XM_001251645.6 , XM_002689674.4
    Accession Version XM_001251645.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product GPI ethanolamine phosphate transferase 3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645279.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001251645.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCTACTG GTCTTTGCAT CACAGTTATG AATTCATCTC ATGACAATTC CAAGATGCAG 
    AAGATCTCAG TGCTGCTTTT CCTGGCCTGG GTGAGCTTCC TCTTCTACGC CGGCATTGCT
    CTCTTCACCA GTGGCTTCCT GCTCACCCGT TGGGAGCTCA CCAACCATAG CAGCTGTCAA
    GAGCCCCCAG GCCCTGGAGC CCTGCCATGG GGGGGCCGAG GGGAACCCGG GGCCTGCTGG
    ATGGCTTCCC GATTCTCTCG GCTCGTGTTG GTGGTGATAG ATGCTCTGCG ATTTGACTTT
    GCCCAGCCCC AGCGCTCACC TGTTTCTGGG GAGCCTCCTG TCTCCCTGCC TTTCCTGGGT
    AAAATGGGCT TCTTGCAGAG ACTCCTGGAA AGTCAGCCCC ACCATGCCAG GCTCTACCAA
    GCCAAGGCTG ACCCCCCGAC CACCACCATG CAGCGCCTCA AGGCCTTAAC CACCGGCTCA
    CTGCCTACCT TTGTTGATGC TGGCAGTAAT TTTGCCAGCA GCGCCATAGT GGAAGATAAT
    CTCATTAAGC AGCTTTCCAG TACAGGAAGA CGTGTGGTCT TCATGGGAGA TGAAACCTGG
    AATGATCTTT TCCCTGGAGT TTTCTCCCAA GCTTTATTCT TCCCATCCTT CAATGTCAGA
    GACCTAGAGA CAGTGGACAA TGGCATCATG AAACACCTCT ATCCAACCAT GGACAGTGAC
    GAATGGGACG TGCTGATTAC TCACTTCCTG GGTGTGGATC ACTGTGGCCA CAAGCATGAC
    CCTTACCATC CTGAAATGGC TAAGAAACTT ACTCAAATGG ATAAAATGAT CCAGGGACTT
    GTGAAGCGTC TGAAGAACGA CACACTGCTG GTGGTGACTG GGGACCATGG GATGACCATC
    AGTGGGAACC ATGGAGGAGA GAGTGAGCTG GAAACCTCAG CTGCACTTTT TCTATATAGT
    CCCAGAGCTC TCTTCCCCAG CGCTCCTGCA GAGGAGCCAG AGATAATTCC TCAAATCAGC
    CTTGTACCTA CGCTGGCCCT GCTGCTGGGC CTGCCCATCC CATTTGGAAA CATCGGGGAG
    GTGATAGCTG AGGTGTTCTC AGAGATCGAA GACTCCCAGC CTCGCTTCTC TGCTCTGGCC
    CAAGCCTCAG CTCTGCATCT CAATGCCCAG CAGGTGTCCC GATTTCTTCA CACCTACTCA
    GCCACTGCTC AGGACCTTCA AGTTCAGGAG CTGCATCGAT TGCAGAAGCT CTTCTCCAAG
    GCTTCTGCTG ACTACCAGCG ACTTCTGCAG AGCCCCCAGG GGGCTGAGGC AGTGCTCCAA
    ACTGTGATTC CTGAGCTGCA GCAGTTCCTG CGGGGAGTTC GGGCCATGTG CATTGAGTCT
    TGGGCTCGTT TTTCTCTGGT CCGCATGGCA GGGGGTGCTG CTCTTCTGGC TGCTACCTGC
    TTTCTTTGCC TACTGGTATC CCAGTGGGCA ACATCCCCAG CCTTCTATTT CGGCCCTCTC
    CTAAAACCCA CAGCCTCGGG TCTGAGTGGC GCCATGGTGT GTGCTGGACT CCTGGCATCT
    ACTGGGCTGA AGCTGGATTC CATGGTTCTA GGGGCCATGG CTGCAGTGGG CTCACTCCTG
    CCTTTTCTGT GGAAAGCTTG GGCGAGCTGG GGATCGAAAA GGCCCCTGGC CTCTCTCCTT
    CCCATGCCCG GGCCAGTCCT ATTACTCCTG CTCATTCGCT TTGCTGGTTT CTTCTCTGAT
    AGCTATATTA TAACCGAAGC CAGGGTTGCC CCCTTCCTTT TGATCTCACT CATCTTGCTC
    CTGGTTGCCC GACTTCACTG GGAGGGCAAG CTGCTGCCAC CTAAGCTACT CTCGATACCT
    CGCCTTGGCT TTTTGACTCC AGCAGGTCCC CCGCAATACA GTGCCATGCA TGCCGTGGGA
    CTTGGAGTTG GGTTGCTTTT ATGTATAAGG CTAGCTGGAC TTTTTTATCG CTGCCATGAA
    GAGACACCTG TTTGTAGCTC CTCTCCCTTG CTGAGTCCCT TGCGATCCAC AGTGGATGGT
    CGAACCAAGA ATTTGTGGTA TGGAGCTTGT GTCGGGGCAC TGGTGGCCCT GTTAGCCTCT
    GTGCGGCTGT GGCTTCACTG CTATGGTAAT CTCAAGAGCC CTGAGCCCCC TGTGCTCTTT
    GTGCGCTGGG GTCTGCCCCT AATGGGACTG GGCACTGCTG CCTACTGGGC ATTGGCATCA
    GGGGCAGATG AAGCACCCCC GCGTCTCCGG GCCCTGGTCT CTGGGGCATC AGTTATGTTG
    CCTCGGGCTG TGGCAGGGTT GGCCGCTTCA GGGCTCATGC TGCTGCTCTG GAGGCCTGTG
    ACAGTGCTGG TGAAGGCCAC AACAGGTGCT CCAAGGACCA GGACTGTCCT CACTCCCTTC
    TCAGGTCCCC CCACTTCTCA GGCTGACTTG GATTATGTGG TTCCTCAAAT CTACCGACAC
    ATACAAGAGG AGTTCCGGGG CCGGCTAGAG AGGACCAAAT CTCAGGGTCT GCTGACGGTG
    GCTGCCTATC AGTTGGGGAG CGTCTACTCA GCTGCTGTGG TTATGGCCCT TACCCTCTTG
    GCCTTCCCAC TTCTGCTATT GCATGCAGAG CGCATTAGCC TTGTGTTCCT GCTTCTGTTT
    CTGCAGAGCT TCCTTCTCCT GCATCTGCTT GCTGCCGGGA TACCCATCAC CACCCCTGGT
    CCTTTTACTG TGCCATGGCA TGCAGTTTTT GCATGGGCCC TCATGGCCAC ACAGACCTTC
    TATACCACCG GCCACCAGCC TGTCTTTTCA GCTATCCAAT GGCATGCAGC CTTCGTGGGA
    GTCTCAGAGA GTCGTGACTT TACCTGGCTG TCTGCTTTCC TGGTGGGAGC CAACACCTTT
    GCCTCCCATA TTCTCTTTGC AGTAGGTTGC CCGTTGCTCC TGCTCTGGCC CTTTCTGTGT
    GAGAATCAAG GATCCCAGAA GAGGCGGCAG CCCCCAGGAA ATGAAGCTGA GGCCAGAGTC
    AGGCCTGAGG AGGAGCAGGA GCCACGGATG GAAATGCGGC TCCGGGATGC ACCTCACCAC
    TTCAGTGCAG CACTGCTGCA GCTGGGCCTC AAGTACCTCT TTGTCCTTGG TATTCAGATT
    CTGGCCTGTG TCTTGGCAGC CTCCATCCTC CGCAGGCATC TCTTGGTCTG GAAGGTGTTT
    GCCCCCAAGT TCATTTTTGA GGCCATGGGC TTCATTGTGA GCAGCGTGGG ACTTTGTCTA
    GGCATAGCTT TGGTGATGCG AGTAGATGGT GCTGTGAGCT CCTGGTTCAG GCAGTTAGTT
    CTGACCCAAC AGAAAGCTGG AGAACAATCT AGCCTGGTCT GTACGGGTGC TGGATCATCT
    GCAAGAAAGG CTCAGCCACA CCTCTTACCA CCATGCAGCC AGGACCTACT GGCATCTGGG
    ACTTCATTAT TTTATAATTC AAGATCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001251646.2
    CDS1007..4456
    Translation

    Target ORF information:

    RefSeq Version XM_001251645.6
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphatidylinositol glycan anchor biosynthesis class O (PIGO), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001251645.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCTACTG GTCTTTGCAT CACAGTTATG AATTCATCTC ATGACAATTC CAAGATGCAG 
    AAGATCTCAG TGCTGCTTTT CCTGGCCTGG GTGAGCTTCC TCTTCTACGC CGGCATTGCT
    CTCTTCACCA GTGGCTTCCT GCTCACCCGT TGGGAGCTCA CCAACCATAG CAGCTGTCAA
    GAGCCCCCAG GCCCTGGAGC CCTGCCATGG GGGGGCCGAG GGGAACCCGG GGCCTGCTGG
    ATGGCTTCCC GATTCTCTCG GCTCGTGTTG GTGGTGATAG ATGCTCTGCG ATTTGACTTT
    GCCCAGCCCC AGCGCTCACC TGTTTCTGGG GAGCCTCCTG TCTCCCTGCC TTTCCTGGGT
    AAAATGGGCT TCTTGCAGAG ACTCCTGGAA AGTCAGCCCC ACCATGCCAG GCTCTACCAA
    GCCAAGGCTG ACCCCCCGAC CACCACCATG CAGCGCCTCA AGGCCTTAAC CACCGGCTCA
    CTGCCTACCT TTGTTGATGC TGGCAGTAAT TTTGCCAGCA GCGCCATAGT GGAAGATAAT
    CTCATTAAGC AGCTTTCCAG TACAGGAAGA CGTGTGGTCT TCATGGGAGA TGAAACCTGG
    AATGATCTTT TCCCTGGAGT TTTCTCCCAA GCTTTATTCT TCCCATCCTT CAATGTCAGA
    GACCTAGAGA CAGTGGACAA TGGCATCATG AAACACCTCT ATCCAACCAT GGACAGTGAC
    GAATGGGACG TGCTGATTAC TCACTTCCTG GGTGTGGATC ACTGTGGCCA CAAGCATGAC
    CCTTACCATC CTGAAATGGC TAAGAAACTT ACTCAAATGG ATAAAATGAT CCAGGGACTT
    GTGAAGCGTC TGAAGAACGA CACACTGCTG GTGGTGACTG GGGACCATGG GATGACCATC
    AGTGGGAACC ATGGAGGAGA GAGTGAGCTG GAAACCTCAG CTGCACTTTT TCTATATAGT
    CCCAGAGCTC TCTTCCCCAG CGCTCCTGCA GAGGAGCCAG AGATAATTCC TCAAATCAGC
    CTTGTACCTA CGCTGGCCCT GCTGCTGGGC CTGCCCATCC CATTTGGAAA CATCGGGGAG
    GTGATAGCTG AGGTGTTCTC AGAGATCGAA GACTCCCAGC CTCGCTTCTC TGCTCTGGCC
    CAAGCCTCAG CTCTGCATCT CAATGCCCAG CAGGTGTCCC GATTTCTTCA CACCTACTCA
    GCCACTGCTC AGGACCTTCA AGTTCAGGAG CTGCATCGAT TGCAGAAGCT CTTCTCCAAG
    GCTTCTGCTG ACTACCAGCG ACTTCTGCAG AGCCCCCAGG GGGCTGAGGC AGTGCTCCAA
    ACTGTGATTC CTGAGCTGCA GCAGTTCCTG CGGGGAGTTC GGGCCATGTG CATTGAGTCT
    TGGGCTCGTT TTTCTCTGGT CCGCATGGCA GGGGGTGCTG CTCTTCTGGC TGCTACCTGC
    TTTCTTTGCC TACTGGTATC CCAGTGGGCA ACATCCCCAG CCTTCTATTT CGGCCCTCTC
    CTAAAACCCA CAGCCTCGGG TCTGAGTGGC GCCATGGTGT GTGCTGGACT CCTGGCATCT
    ACTGGGCTGA AGCTGGATTC CATGGTTCTA GGGGCCATGG CTGCAGTGGG CTCACTCCTG
    CCTTTTCTGT GGAAAGCTTG GGCGAGCTGG GGATCGAAAA GGCCCCTGGC CTCTCTCCTT
    CCCATGCCCG GGCCAGTCCT ATTACTCCTG CTCATTCGCT TTGCTGGTTT CTTCTCTGAT
    AGCTATATTA TAACCGAAGC CAGGGTTGCC CCCTTCCTTT TGATCTCACT CATCTTGCTC
    CTGGTTGCCC GACTTCACTG GGAGGGCAAG CTGCTGCCAC CTAAGCTACT CTCGATACCT
    CGCCTTGGCT TTTTGACTCC AGCAGGTCCC CCGCAATACA GTGCCATGCA TGCCGTGGGA
    CTTGGAGTTG GGTTGCTTTT ATGTATAAGG CTAGCTGGAC TTTTTTATCG CTGCCATGAA
    GAGACACCTG TTTGTAGCTC CTCTCCCTTG CTGAGTCCCT TGCGATCCAC AGTGGATGGT
    CGAACCAAGA ATTTGTGGTA TGGAGCTTGT GTCGGGGCAC TGGTGGCCCT GTTAGCCTCT
    GTGCGGCTGT GGCTTCACTG CTATGGTAAT CTCAAGAGCC CTGAGCCCCC TGTGCTCTTT
    GTGCGCTGGG GTCTGCCCCT AATGGGACTG GGCACTGCTG CCTACTGGGC ATTGGCATCA
    GGGGCAGATG AAGCACCCCC GCGTCTCCGG GCCCTGGTCT CTGGGGCATC AGTTATGTTG
    CCTCGGGCTG TGGCAGGGTT GGCCGCTTCA GGGCTCATGC TGCTGCTCTG GAGGCCTGTG
    ACAGTGCTGG TGAAGGCCAC AACAGGTGCT CCAAGGACCA GGACTGTCCT CACTCCCTTC
    TCAGGTCCCC CCACTTCTCA GGCTGACTTG GATTATGTGG TTCCTCAAAT CTACCGACAC
    ATACAAGAGG AGTTCCGGGG CCGGCTAGAG AGGACCAAAT CTCAGGGTCT GCTGACGGTG
    GCTGCCTATC AGTTGGGGAG CGTCTACTCA GCTGCTGTGG TTATGGCCCT TACCCTCTTG
    GCCTTCCCAC TTCTGCTATT GCATGCAGAG CGCATTAGCC TTGTGTTCCT GCTTCTGTTT
    CTGCAGAGCT TCCTTCTCCT GCATCTGCTT GCTGCCGGGA TACCCATCAC CACCCCTGGT
    CCTTTTACTG TGCCATGGCA TGCAGTTTTT GCATGGGCCC TCATGGCCAC ACAGACCTTC
    TATACCACCG GCCACCAGCC TGTCTTTTCA GCTATCCAAT GGCATGCAGC CTTCGTGGGA
    GTCTCAGAGA GTCGTGACTT TACCTGGCTG TCTGCTTTCC TGGTGGGAGC CAACACCTTT
    GCCTCCCATA TTCTCTTTGC AGTAGGTTGC CCGTTGCTCC TGCTCTGGCC CTTTCTGTGT
    GAGAATCAAG GATCCCAGAA GAGGCGGCAG CCCCCAGGAA ATGAAGCTGA GGCCAGAGTC
    AGGCCTGAGG AGGAGCAGGA GCCACGGATG GAAATGCGGC TCCGGGATGC ACCTCACCAC
    TTCAGTGCAG CACTGCTGCA GCTGGGCCTC AAGTACCTCT TTGTCCTTGG TATTCAGATT
    CTGGCCTGTG TCTTGGCAGC CTCCATCCTC CGCAGGCATC TCTTGGTCTG GAAGGTGTTT
    GCCCCCAAGT TCATTTTTGA GGCCATGGGC TTCATTGTGA GCAGCGTGGG ACTTTGTCTA
    GGCATAGCTT TGGTGATGCG AGTAGATGGT GCTGTGAGCT CCTGGTTCAG GCAGTTAGTT
    CTGACCCAAC AGAAAGCTGG AGAACAATCT AGCCTGGTCT GTACGGGTGC TGGATCATCT
    GCAAGAAAGG CTCAGCCACA CCTCTTACCA CCATGCAGCC AGGACCTACT GGCATCTGGG
    ACTTCATTAT TTTATAATTC AAGATCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91283
    Clone ID Related Accession (Same CDS sequence) XM_001251645.6 , XM_002689674.4
    Accession Version XM_002689674.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product GPI ethanolamine phosphate transferase 3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002689674.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCTACTG GTCTTTGCAT CACAGTTATG AATTCATCTC ATGACAATTC CAAGATGCAG 
    AAGATCTCAG TGCTGCTTTT CCTGGCCTGG GTGAGCTTCC TCTTCTACGC CGGCATTGCT
    CTCTTCACCA GTGGCTTCCT GCTCACCCGT TGGGAGCTCA CCAACCATAG CAGCTGTCAA
    GAGCCCCCAG GCCCTGGAGC CCTGCCATGG GGGGGCCGAG GGGAACCCGG GGCCTGCTGG
    ATGGCTTCCC GATTCTCTCG GCTCGTGTTG GTGGTGATAG ATGCTCTGCG ATTTGACTTT
    GCCCAGCCCC AGCGCTCACC TGTTTCTGGG GAGCCTCCTG TCTCCCTGCC TTTCCTGGGT
    AAAATGGGCT TCTTGCAGAG ACTCCTGGAA AGTCAGCCCC ACCATGCCAG GCTCTACCAA
    GCCAAGGCTG ACCCCCCGAC CACCACCATG CAGCGCCTCA AGGCCTTAAC CACCGGCTCA
    CTGCCTACCT TTGTTGATGC TGGCAGTAAT TTTGCCAGCA GCGCCATAGT GGAAGATAAT
    CTCATTAAGC AGCTTTCCAG TACAGGAAGA CGTGTGGTCT TCATGGGAGA TGAAACCTGG
    AATGATCTTT TCCCTGGAGT TTTCTCCCAA GCTTTATTCT TCCCATCCTT CAATGTCAGA
    GACCTAGAGA CAGTGGACAA TGGCATCATG AAACACCTCT ATCCAACCAT GGACAGTGAC
    GAATGGGACG TGCTGATTAC TCACTTCCTG GGTGTGGATC ACTGTGGCCA CAAGCATGAC
    CCTTACCATC CTGAAATGGC TAAGAAACTT ACTCAAATGG ATAAAATGAT CCAGGGACTT
    GTGAAGCGTC TGAAGAACGA CACACTGCTG GTGGTGACTG GGGACCATGG GATGACCATC
    AGTGGGAACC ATGGAGGAGA GAGTGAGCTG GAAACCTCAG CTGCACTTTT TCTATATAGT
    CCCAGAGCTC TCTTCCCCAG CGCTCCTGCA GAGGAGCCAG AGATAATTCC TCAAATCAGC
    CTTGTACCTA CGCTGGCCCT GCTGCTGGGC CTGCCCATCC CATTTGGAAA CATCGGGGAG
    GTGATAGCTG AGGTGTTCTC AGAGATCGAA GACTCCCAGC CTCGCTTCTC TGCTCTGGCC
    CAAGCCTCAG CTCTGCATCT CAATGCCCAG CAGGTGTCCC GATTTCTTCA CACCTACTCA
    GCCACTGCTC AGGACCTTCA AGTTCAGGAG CTGCATCGAT TGCAGAAGCT CTTCTCCAAG
    GCTTCTGCTG ACTACCAGCG ACTTCTGCAG AGCCCCCAGG GGGCTGAGGC AGTGCTCCAA
    ACTGTGATTC CTGAGCTGCA GCAGTTCCTG CGGGGAGTTC GGGCCATGTG CATTGAGTCT
    TGGGCTCGTT TTTCTCTGGT CCGCATGGCA GGGGGTGCTG CTCTTCTGGC TGCTACCTGC
    TTTCTTTGCC TACTGGTATC CCAGTGGGCA ACATCCCCAG CCTTCTATTT CGGCCCTCTC
    CTAAAACCCA CAGCCTCGGG TCTGAGTGGC GCCATGGTGT GTGCTGGACT CCTGGCATCT
    ACTGGGCTGA AGCTGGATTC CATGGTTCTA GGGGCCATGG CTGCAGTGGG CTCACTCCTG
    CCTTTTCTGT GGAAAGCTTG GGCGAGCTGG GGATCGAAAA GGCCCCTGGC CTCTCTCCTT
    CCCATGCCCG GGCCAGTCCT ATTACTCCTG CTCATTCGCT TTGCTGGTTT CTTCTCTGAT
    AGCTATATTA TAACCGAAGC CAGGGTTGCC CCCTTCCTTT TGATCTCACT CATCTTGCTC
    CTGGTTGCCC GACTTCACTG GGAGGGCAAG CTGCTGCCAC CTAAGCTACT CTCGATACCT
    CGCCTTGGCT TTTTGACTCC AGCAGGTCCC CCGCAATACA GTGCCATGCA TGCCGTGGGA
    CTTGGAGTTG GGTTGCTTTT ATGTATAAGG CTAGCTGGAC TTTTTTATCG CTGCCATGAA
    GAGACACCTG TTTGTAGCTC CTCTCCCTTG CTGAGTCCCT TGCGATCCAC AGTGGATGGT
    CGAACCAAGA ATTTGTGGTA TGGAGCTTGT GTCGGGGCAC TGGTGGCCCT GTTAGCCTCT
    GTGCGGCTGT GGCTTCACTG CTATGGTAAT CTCAAGAGCC CTGAGCCCCC TGTGCTCTTT
    GTGCGCTGGG GTCTGCCCCT AATGGGACTG GGCACTGCTG CCTACTGGGC ATTGGCATCA
    GGGGCAGATG AAGCACCCCC GCGTCTCCGG GCCCTGGTCT CTGGGGCATC AGTTATGTTG
    CCTCGGGCTG TGGCAGGGTT GGCCGCTTCA GGGCTCATGC TGCTGCTCTG GAGGCCTGTG
    ACAGTGCTGG TGAAGGCCAC AACAGGTGCT CCAAGGACCA GGACTGTCCT CACTCCCTTC
    TCAGGTCCCC CCACTTCTCA GGCTGACTTG GATTATGTGG TTCCTCAAAT CTACCGACAC
    ATACAAGAGG AGTTCCGGGG CCGGCTAGAG AGGACCAAAT CTCAGGGTCT GCTGACGGTG
    GCTGCCTATC AGTTGGGGAG CGTCTACTCA GCTGCTGTGG TTATGGCCCT TACCCTCTTG
    GCCTTCCCAC TTCTGCTATT GCATGCAGAG CGCATTAGCC TTGTGTTCCT GCTTCTGTTT
    CTGCAGAGCT TCCTTCTCCT GCATCTGCTT GCTGCCGGGA TACCCATCAC CACCCCTGGT
    CCTTTTACTG TGCCATGGCA TGCAGTTTTT GCATGGGCCC TCATGGCCAC ACAGACCTTC
    TATACCACCG GCCACCAGCC TGTCTTTTCA GCTATCCAAT GGCATGCAGC CTTCGTGGGA
    GTCTCAGAGA GTCGTGACTT TACCTGGCTG TCTGCTTTCC TGGTGGGAGC CAACACCTTT
    GCCTCCCATA TTCTCTTTGC AGTAGGTTGC CCGTTGCTCC TGCTCTGGCC CTTTCTGTGT
    GAGAATCAAG GATCCCAGAA GAGGCGGCAG CCCCCAGGAA ATGAAGCTGA GGCCAGAGTC
    AGGCCTGAGG AGGAGCAGGA GCCACGGATG GAAATGCGGC TCCGGGATGC ACCTCACCAC
    TTCAGTGCAG CACTGCTGCA GCTGGGCCTC AAGTACCTCT TTGTCCTTGG TATTCAGATT
    CTGGCCTGTG TCTTGGCAGC CTCCATCCTC CGCAGGCATC TCTTGGTCTG GAAGGTGTTT
    GCCCCCAAGT TCATTTTTGA GGCCATGGGC TTCATTGTGA GCAGCGTGGG ACTTTGTCTA
    GGCATAGCTT TGGTGATGCG AGTAGATGGT GCTGTGAGCT CCTGGTTCAG GCAGTTAGTT
    CTGACCCAAC AGAAAGCTGG AGAACAATCT AGCCTGGTCT GTACGGGTGC TGGATCATCT
    GCAAGAAAGG CTCAGCCACA CCTCTTACCA CCATGCAGCC AGGACCTACT GGCATCTGGG
    ACTTCATTAT TTTATAATTC AAGATCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002689720.2
    CDS1007..4456
    Translation

    Target ORF information:

    RefSeq Version XM_002689674.4
    Organism Bos taurus(cattle)
    Definition Bos taurus phosphatidylinositol glycan anchor biosynthesis class O (PIGO), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002689674.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCTACTG GTCTTTGCAT CACAGTTATG AATTCATCTC ATGACAATTC CAAGATGCAG 
    AAGATCTCAG TGCTGCTTTT CCTGGCCTGG GTGAGCTTCC TCTTCTACGC CGGCATTGCT
    CTCTTCACCA GTGGCTTCCT GCTCACCCGT TGGGAGCTCA CCAACCATAG CAGCTGTCAA
    GAGCCCCCAG GCCCTGGAGC CCTGCCATGG GGGGGCCGAG GGGAACCCGG GGCCTGCTGG
    ATGGCTTCCC GATTCTCTCG GCTCGTGTTG GTGGTGATAG ATGCTCTGCG ATTTGACTTT
    GCCCAGCCCC AGCGCTCACC TGTTTCTGGG GAGCCTCCTG TCTCCCTGCC TTTCCTGGGT
    AAAATGGGCT TCTTGCAGAG ACTCCTGGAA AGTCAGCCCC ACCATGCCAG GCTCTACCAA
    GCCAAGGCTG ACCCCCCGAC CACCACCATG CAGCGCCTCA AGGCCTTAAC CACCGGCTCA
    CTGCCTACCT TTGTTGATGC TGGCAGTAAT TTTGCCAGCA GCGCCATAGT GGAAGATAAT
    CTCATTAAGC AGCTTTCCAG TACAGGAAGA CGTGTGGTCT TCATGGGAGA TGAAACCTGG
    AATGATCTTT TCCCTGGAGT TTTCTCCCAA GCTTTATTCT TCCCATCCTT CAATGTCAGA
    GACCTAGAGA CAGTGGACAA TGGCATCATG AAACACCTCT ATCCAACCAT GGACAGTGAC
    GAATGGGACG TGCTGATTAC TCACTTCCTG GGTGTGGATC ACTGTGGCCA CAAGCATGAC
    CCTTACCATC CTGAAATGGC TAAGAAACTT ACTCAAATGG ATAAAATGAT CCAGGGACTT
    GTGAAGCGTC TGAAGAACGA CACACTGCTG GTGGTGACTG GGGACCATGG GATGACCATC
    AGTGGGAACC ATGGAGGAGA GAGTGAGCTG GAAACCTCAG CTGCACTTTT TCTATATAGT
    CCCAGAGCTC TCTTCCCCAG CGCTCCTGCA GAGGAGCCAG AGATAATTCC TCAAATCAGC
    CTTGTACCTA CGCTGGCCCT GCTGCTGGGC CTGCCCATCC CATTTGGAAA CATCGGGGAG
    GTGATAGCTG AGGTGTTCTC AGAGATCGAA GACTCCCAGC CTCGCTTCTC TGCTCTGGCC
    CAAGCCTCAG CTCTGCATCT CAATGCCCAG CAGGTGTCCC GATTTCTTCA CACCTACTCA
    GCCACTGCTC AGGACCTTCA AGTTCAGGAG CTGCATCGAT TGCAGAAGCT CTTCTCCAAG
    GCTTCTGCTG ACTACCAGCG ACTTCTGCAG AGCCCCCAGG GGGCTGAGGC AGTGCTCCAA
    ACTGTGATTC CTGAGCTGCA GCAGTTCCTG CGGGGAGTTC GGGCCATGTG CATTGAGTCT
    TGGGCTCGTT TTTCTCTGGT CCGCATGGCA GGGGGTGCTG CTCTTCTGGC TGCTACCTGC
    TTTCTTTGCC TACTGGTATC CCAGTGGGCA ACATCCCCAG CCTTCTATTT CGGCCCTCTC
    CTAAAACCCA CAGCCTCGGG TCTGAGTGGC GCCATGGTGT GTGCTGGACT CCTGGCATCT
    ACTGGGCTGA AGCTGGATTC CATGGTTCTA GGGGCCATGG CTGCAGTGGG CTCACTCCTG
    CCTTTTCTGT GGAAAGCTTG GGCGAGCTGG GGATCGAAAA GGCCCCTGGC CTCTCTCCTT
    CCCATGCCCG GGCCAGTCCT ATTACTCCTG CTCATTCGCT TTGCTGGTTT CTTCTCTGAT
    AGCTATATTA TAACCGAAGC CAGGGTTGCC CCCTTCCTTT TGATCTCACT CATCTTGCTC
    CTGGTTGCCC GACTTCACTG GGAGGGCAAG CTGCTGCCAC CTAAGCTACT CTCGATACCT
    CGCCTTGGCT TTTTGACTCC AGCAGGTCCC CCGCAATACA GTGCCATGCA TGCCGTGGGA
    CTTGGAGTTG GGTTGCTTTT ATGTATAAGG CTAGCTGGAC TTTTTTATCG CTGCCATGAA
    GAGACACCTG TTTGTAGCTC CTCTCCCTTG CTGAGTCCCT TGCGATCCAC AGTGGATGGT
    CGAACCAAGA ATTTGTGGTA TGGAGCTTGT GTCGGGGCAC TGGTGGCCCT GTTAGCCTCT
    GTGCGGCTGT GGCTTCACTG CTATGGTAAT CTCAAGAGCC CTGAGCCCCC TGTGCTCTTT
    GTGCGCTGGG GTCTGCCCCT AATGGGACTG GGCACTGCTG CCTACTGGGC ATTGGCATCA
    GGGGCAGATG AAGCACCCCC GCGTCTCCGG GCCCTGGTCT CTGGGGCATC AGTTATGTTG
    CCTCGGGCTG TGGCAGGGTT GGCCGCTTCA GGGCTCATGC TGCTGCTCTG GAGGCCTGTG
    ACAGTGCTGG TGAAGGCCAC AACAGGTGCT CCAAGGACCA GGACTGTCCT CACTCCCTTC
    TCAGGTCCCC CCACTTCTCA GGCTGACTTG GATTATGTGG TTCCTCAAAT CTACCGACAC
    ATACAAGAGG AGTTCCGGGG CCGGCTAGAG AGGACCAAAT CTCAGGGTCT GCTGACGGTG
    GCTGCCTATC AGTTGGGGAG CGTCTACTCA GCTGCTGTGG TTATGGCCCT TACCCTCTTG
    GCCTTCCCAC TTCTGCTATT GCATGCAGAG CGCATTAGCC TTGTGTTCCT GCTTCTGTTT
    CTGCAGAGCT TCCTTCTCCT GCATCTGCTT GCTGCCGGGA TACCCATCAC CACCCCTGGT
    CCTTTTACTG TGCCATGGCA TGCAGTTTTT GCATGGGCCC TCATGGCCAC ACAGACCTTC
    TATACCACCG GCCACCAGCC TGTCTTTTCA GCTATCCAAT GGCATGCAGC CTTCGTGGGA
    GTCTCAGAGA GTCGTGACTT TACCTGGCTG TCTGCTTTCC TGGTGGGAGC CAACACCTTT
    GCCTCCCATA TTCTCTTTGC AGTAGGTTGC CCGTTGCTCC TGCTCTGGCC CTTTCTGTGT
    GAGAATCAAG GATCCCAGAA GAGGCGGCAG CCCCCAGGAA ATGAAGCTGA GGCCAGAGTC
    AGGCCTGAGG AGGAGCAGGA GCCACGGATG GAAATGCGGC TCCGGGATGC ACCTCACCAC
    TTCAGTGCAG CACTGCTGCA GCTGGGCCTC AAGTACCTCT TTGTCCTTGG TATTCAGATT
    CTGGCCTGTG TCTTGGCAGC CTCCATCCTC CGCAGGCATC TCTTGGTCTG GAAGGTGTTT
    GCCCCCAAGT TCATTTTTGA GGCCATGGGC TTCATTGTGA GCAGCGTGGG ACTTTGTCTA
    GGCATAGCTT TGGTGATGCG AGTAGATGGT GCTGTGAGCT CCTGGTTCAG GCAGTTAGTT
    CTGACCCAAC AGAAAGCTGG AGAACAATCT AGCCTGGTCT GTACGGGTGC TGGATCATCT
    GCAAGAAAGG CTCAGCCACA CCTCTTACCA CCATGCAGCC AGGACCTACT GGCATCTGGG
    ACTTCATTAT TTTATAATTC AAGATCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.