SELENOT cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SELENOT
Entrez Gene ID	783831
Full Name	selenoprotein T
Synonyms	SELT
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Summary	This gene encodes a selenoprotein, containing a selenocysteine (Sec) residue at the active site. Sec is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is localized in the endoplasmic reticulum. It belongs to the SelWTH family that possesses a thioredoxin-like fold and a conserved CxxU (C is cysteine, U is Sec) motif, suggesting a redox function for this gene. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103103.2	NP_001096573.2	thioredoxin reductase-like selenoprotein T precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SELT	NP_001096573.2
Mus musculus (house mouse)	Selt	NP_001035486.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009202	XP_319979.4
Canis lupus familiaris (dog)	SELT	NP_001157959.1
Homo sapiens (human)	SELT	NP_057359.2
Danio rerio (zebrafish)	selt1a	NP_840075.2
Rattus norvegicus (Norway rat)	Selt	NP_001014275.2
Gallus gallus (chicken)	SELT	NP_001006557.3
Xenopus tropicalis (tropical clawed frog)	selt	NP_988868.2
	SELT	NP_001152886.1
Drosophila melanogaster (fruit fly)	CG3887	NP_608897.1

Gene Ontology
Bibliography

Related articles in PubMed

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of mammalian selenoproteomes.
Kryukov GV, Castellano S, Novoselov SV, Lobanov AV, Zehtab O, Guig? R, Gladyshev VN
Science (New York, N.Y.)300(5624)1439-43(2003 May)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SELENOT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SELENOT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa43217	NM_001103103.2 Latest version!	Bos taurus selenoprotein T (SELENOT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SELENOT ( NM_001103103.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001096573.2 Bos taurus selenoprotein T (SELENOT), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OBa43217
Clone ID Related Accession (Same CDS sequence)	NM_001103103.2
Accession Version	NM_001103103.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Bos taurus(cattle)
Product	thioredoxin reductase-like selenoprotein T precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DT887648.1, BC149082.1 and DV816938.1. On Feb 22, 2008 this sequence version replaced NM_001103103.1. Transcript Variant: This variant (1) represents the predominant transcript and encodes a selenoprotein. ##Evidence-Data-START## Transcript exon combination :: BC149082.1, DV794689.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGGCTTC TGCTACTTCT GCTGGTGGCG GCGTCGGCCG TGGTCCGGAG CGATGCCTCG 
GCCAACTTGG GCGGCGTGCC TGGCAAGAGA TTAAAGATGC AGTACGCCAC GGGGCCTCTG 
CTCAAGTTCC AGATTTGTGT TTCCTGAGGT TACAGGCGGG TGTTTGAGGA GTACATGCGG 
GTTATTAGCC AGCGGTACCC TGACATCCGC ATAGAAGGAG AGAATTACCT CCCTCAACCA 
ATATATAGAC ACATAGCATC TTTCCTGTCA GTCTTCAAGC TAGTATTAAT AGGCTTAATA 
ATTGTTGGCA AGGATCCTTT TGCTTTCTTT GGCATGCAAG CTCCTAGCAT CTGGCAGTGG 
GGCCAAGAAA ATAAGGTCTA TGCATGTATG ATGGTATTCT TCTTGAGCAA CATGATTGAA 
AACCAGTGTA TGTCAACAGG TGCATTTGAG ATAACTTTGA ATGATGTACC TGTGTGGTCT 
AAGTTGGAAT CTGGTCACCT TCCATCCATG CAACAGCTGG TTCAAATTCT TGACAATGAA 
ATGAAGCTCA ACGTGCATAT GGATTCAATC CCACACCATC GATCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096573.2
CDS	115..702
Translation	MRLLLLLLVAASAVVRSDASANLGGVPGKRLKMQYATGPLLKFQICVS*GYRRVFEEYMRVISQRYPDIRIEGENYLPQPIYRHIASFLSVFKLVLIGLIIVGKDPFAFFGMQAPSIWQWGQENKVYACMMVFFLSNMIENQCMSTGAFEITLNDVPVWSKLESGHLPSMQQLVQILDNEMKLNVHMDSIPHHRS

Target ORF information:

RefSeq Version	NM_001103103.2
Organism	Bos taurus(cattle)
Definition	Bos taurus selenoprotein T (SELENOT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103103.2

ORF Insert Sequence:

ATGAGGCTTC TGCTACTTCT GCTGGTGGCG GCGTCGGCCG TGGTCCGGAG CGATGCCTCG 
GCCAACTTGG GCGGCGTGCC TGGCAAGAGA TTAAAGATGC AGTACGCCAC GGGGCCTCTG 
CTCAAGTTCC AGATTTGTGT TTCCTGAGGT TACAGGCGGG TGTTTGAGGA GTACATGCGG 
GTTATTAGCC AGCGGTACCC TGACATCCGC ATAGAAGGAG AGAATTACCT CCCTCAACCA 
ATATATAGAC ACATAGCATC TTTCCTGTCA GTCTTCAAGC TAGTATTAAT AGGCTTAATA 
ATTGTTGGCA AGGATCCTTT TGCTTTCTTT GGCATGCAAG CTCCTAGCAT CTGGCAGTGG 
GGCCAAGAAA ATAAGGTCTA TGCATGTATG ATGGTATTCT TCTTGAGCAA CATGATTGAA 
AACCAGTGTA TGTCAACAGG TGCATTTGAG ATAACTTTGA ATGATGTACC TGTGTGGTCT 
AAGTTGGAAT CTGGTCACCT TCCATCCATG CAACAGCTGG TTCAAATTCT TGACAATGAA 
ATGAAGCTCA ACGTGCATAT GGATTCAATC CCACACCATC GATCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of mammalian selenoproteomes.
Science (New York, N.Y.)300(5624)1439-43(2003 May)
Kryukov GV,Castellano S,Novoselov SV,Lobanov AV,Zehtab O,Guig? R,Gladyshev VN

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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SELENOT cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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