VCL cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	VCL
Entrez Gene ID	783645
Full Name	vinculin
General protein information	Preferred Names vinculin Names vinculin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005226482.2	XP_005226539.1	vinculin isoform X2
XM_015461024.1	XP_015316510.1	vinculin isoform X4
NM_001191370.1	NP_001178299.1	vinculin
XM_005226481.2	XP_005226538.1	vinculin isoform X1
XM_005226483.2	XP_005226540.1	vinculin isoform X3
XM_024986796.1	XP_024842564.1	vinculin isoform X2
XM_005226483.3	XP_005226540.1	vinculin isoform X1

Pathways from BioSystems

Homologies

	VCL	XP_001118118.1
Gallus gallus (chicken)	VCL	NP_990772.1
Xenopus tropicalis (tropical clawed frog)	vcl	NP_001090722.1
Bos taurus (cattle)	VCL	NP_001178299.1
Canis lupus familiaris (dog)	VCL	XP_536395.3
Danio rerio (zebrafish)	vcla	NP_001122153.1
Homo sapiens (human)	VCL	NP_054706.1
Pan troglodytes (chimpanzee)	VCL	XP_507854.2
Mus musculus (house mouse)	Vcl	NP_033528.3
Rattus norvegicus (Norway rat)	Vcl	NP_001100718.1

Gene Ontology

The following VCL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VCL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa231682	XM_024986796.1 Latest version!	Bos taurus vinculin (VCL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa88940	XM_015461024.1 Latest version!	Bos taurus vinculin (VCL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa37453	NM_001191370.1 Latest version!	Bos taurus vinculin (VCL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa34284	XM_005226481.2 Latest version!	Bos taurus vinculin (VCL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa34285	XM_005226482.2 Latest version!	Bos taurus vinculin (VCL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa34286	XM_005226483.3 Latest version!	Bos taurus vinculin (VCL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa34286	XM_005226483.2	Bos taurus vinculin (VCL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VCL ( XM_024986796.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842564.1 Bos taurus vinculin (VCL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa231682
Clone ID Related Accession (Same CDS sequence)	XM_024986796.1
Accession Version	XM_024986796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	vinculin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAATG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGATCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCTCC CCAGGTGATG CTGGTGAACA GGCCATCAGG 
CAGATTTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTGGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAAGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGA CAAGGTAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAGGTGGC CACAGCCCTA CAGAACTTAC AGACTAAAAC AAACAGGGCC 
GTAGCCAACA GCAGACCTGC CAAAGCAGCG GTCCACCTTG AAGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC TACTGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGA 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAGGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGCCC TCAGGCGAGA GCTCTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACGA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACAG CAGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCCCGAGAA CTTACACCCC AGGTGGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGACACTAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTTGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTC GCCGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGAATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAATTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGGTGGAT 
GCAAAGGCCG TGGCAGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTAGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC ACAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTCCGAC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCACC ACCGGAGGAG 
AAGGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCTCGC AAGTGGTCCA GCAAGCCGGG TAACCCAGCC 
GCTAAGGTGG GTATAAGTGT TGTAGCTGAG GCAGAGGCGG CTGATGCTGT TGGGTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG TCATGAAATC CTGGAATGAT CCCAGGTCTG CAGTGGAAGA GGTGCAACCT 
TGTCTAACAT ACTCCTGTTC TCATGAGTTC TTCCCAAACT GTGTTCTCTC TGGCCTCGAC 
GACTGCCTGG GAGGTTACGC ACCCCCTCGT GTAAATGATC AGCCACTTCC TGGTGCCTGT 
GGATGCCTCT CACAGAGCAC CCTGCCGTGG CATGGTTTGT GTATGCCTCA CTGCATTGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842564.1
CDS	119..3301
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVVDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGNPAAKVGISVVAEAEAADAVGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKVMKSWNDPRSAVEEVQPCLTYSCSHEFFPNCVLSGLDDCLGGYAPPRVNDQPLPGACGCLSQSTLPWHGLCMPHCIV

Target ORF information:

RefSeq Version	XM_024986796.1
Organism	Bos taurus(cattle)
Definition	Bos taurus vinculin (VCL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986796.1

ORF Insert Sequence:

ATGCCGGTGT TTCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAATG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGATCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCTCC CCAGGTGATG CTGGTGAACA GGCCATCAGG 
CAGATTTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTGGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAAGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGA CAAGGTAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAGGTGGC CACAGCCCTA CAGAACTTAC AGACTAAAAC AAACAGGGCC 
GTAGCCAACA GCAGACCTGC CAAAGCAGCG GTCCACCTTG AAGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC TACTGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGA 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAGGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGCCC TCAGGCGAGA GCTCTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACGA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACAG CAGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCCCGAGAA CTTACACCCC AGGTGGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGACACTAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTTGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTC GCCGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGAATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAATTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGGTGGAT 
GCAAAGGCCG TGGCAGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTAGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC ACAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTCCGAC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCACC ACCGGAGGAG 
AAGGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCTCGC AAGTGGTCCA GCAAGCCGGG TAACCCAGCC 
GCTAAGGTGG GTATAAGTGT TGTAGCTGAG GCAGAGGCGG CTGATGCTGT TGGGTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG TCATGAAATC CTGGAATGAT CCCAGGTCTG CAGTGGAAGA GGTGCAACCT 
TGTCTAACAT ACTCCTGTTC TCATGAGTTC TTCCCAAACT GTGTTCTCTC TGGCCTCGAC 
GACTGCCTGG GAGGTTACGC ACCCCCTCGT GTAAATGATC AGCCACTTCC TGGTGCCTGT 
GGATGCCTCT CACAGAGCAC CCTGCCGTGG CATGGTTTGT GTATGCCTCA CTGCATTGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_015461024.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316510.1 Bos taurus vinculin (VCL), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa88940
Clone ID Related Accession (Same CDS sequence)	XM_015461024.1
Accession Version	XM_015461024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	vinculin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAATG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGATCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCTAAAGGTT GGCTCCGTGA TCCCAATGCC TCCCCAGGTG ATGCTGGTGA ACAGGCCATC 
AGGCAGATTT TAGATGAAGC TGGAAAAGTT GGTGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCCTGG GAACCTGCAA AATGCTGGGG CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGAGCCTC ACCGGTGGCC ATGCAGAAAG CCCAGCAAGT GTCTCAGGGT 
CTGGATGTGC TCACAGCAAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGAGAT GACATTCTGC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGG AGATCTACGA AGACAAGGTA AAGGAGATTC CCCAGAGGCC 
CGAGCATTAG CCAAACAGGT GGCCACAGCC CTACAGAACT TACAGACTAA AACAAACAGG 
GCCGTAGCCA ACAGCAGACC TGCCAAAGCA GCGGTCCACC TTGAAGGCAA GATTGAGCAA 
GCGCAGCGGT GGATTGATAA TCCTACTGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGAGGGCTTG TGGCCGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAG 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG CCCTCAGGCG AGAGCTCTTG CATCTCAGCT CCAAGACTCC 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGTGATACCA CGACTCCCAT CAAGCTGTTG GCAGTGGCAG CCACTGCTCC TCCTGATGCA 
CCCAATAGGG AAGAGGTATT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAGG 
CTTGGGGCCA CAGCAGAGAA GGCGGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CCTCAGTGAA GACAGCCCGA GAACTTACAC CCCAGGTGGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TCGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGACACT 
AAATCTCTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ACCTGGACAA GTGTAAAGTT 
GCCATGGCCA ACATTCAGCC TCAGATGCTG GTCGCCGGGG CAACCAGCAT TGCTCGTCGG 
GCCAACCGAA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAATTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAA TTGAGCAAAA CCATCTCCCC AATGGTGGTG 
GATGCAAAGG CCGTGGCAGG AAACATTTCT GACCCTGGAC TGCAAAAGAG CTTCCTGGAC 
TCAGGATACC GGATCCTAGG AGCTGTGGCC AAGGTCAGAG AAGCCTTCCA ACCACAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAACAGCTCC GACTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGTGAG GTCCCTCCAC CCAGGCCCCC ACCACCGGAG 
GAGAAGGATG AAGAATTCCC TGAGCAGAAA GCCGGGGAGG TGATTAACCA GCCAATGATG 
ATGGCTGCCA GGCAGCTCCA CGATGAAGCT CGCAAGTGGT CCAGCAAGCC GGGTAACCCA 
GCCGCTAAGG TGGGTATAAG TGTTGTAGCT GAGGCAGAGG CGGCTGATGC TGTTGGGTTC 
CCTGTCCCCC CTGACATGGA AGACGATTAC GAACCTGAGC TGCTGTTAAT GCCATCCAAT 
CAGCCGGTCA ACCAGCCCAT TCTGGCCGCG GCTCAGTCAT TGCATCGGGA AGCTACCAAG 
TGGTCTAGTA AGGTCATGAA ATCCTGGAAT GATCCCAGGT CTGCAGTGGA AGAGGTGCAA 
CCTTGTCTAA CATACTCCTG TTCTCATGAG TTCTTCCCAA ACTGTGTTCT CTCTGGCCTC 
GACGACTGCC TGGGAGGTTA CGCACCCCCT CGTGTAAATG ATCAGCCACT TCCTGGTGCC 
TGTGGATGCC TCTCACAGAG CACCCTGCCG TGGCATGGTT TGTGTATGCC TCACTGCATT 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316510.1
CDS	119..3304
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVVDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGNPAAKVGISVVAEAEAADAVGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKVMKSWNDPRSAVEEVQPCLTYSCSHEFFPNCVLSGLDDCLGGYAPPRVNDQPLPGACGCLSQSTLPWHGLCMPHCIV

Target ORF information:

RefSeq Version	XM_015461024.1
Organism	Bos taurus(cattle)
Definition	Bos taurus vinculin (VCL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461024.1

ORF Insert Sequence:

ATGCCGGTGT TTCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAATG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGATCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCTAAAGGTT GGCTCCGTGA TCCCAATGCC TCCCCAGGTG ATGCTGGTGA ACAGGCCATC 
AGGCAGATTT TAGATGAAGC TGGAAAAGTT GGTGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCCTGG GAACCTGCAA AATGCTGGGG CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGAGCCTC ACCGGTGGCC ATGCAGAAAG CCCAGCAAGT GTCTCAGGGT 
CTGGATGTGC TCACAGCAAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGAGAT GACATTCTGC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGG AGATCTACGA AGACAAGGTA AAGGAGATTC CCCAGAGGCC 
CGAGCATTAG CCAAACAGGT GGCCACAGCC CTACAGAACT TACAGACTAA AACAAACAGG 
GCCGTAGCCA ACAGCAGACC TGCCAAAGCA GCGGTCCACC TTGAAGGCAA GATTGAGCAA 
GCGCAGCGGT GGATTGATAA TCCTACTGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGAGGGCTTG TGGCCGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAG 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG CCCTCAGGCG AGAGCTCTTG CATCTCAGCT CCAAGACTCC 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGTGATACCA CGACTCCCAT CAAGCTGTTG GCAGTGGCAG CCACTGCTCC TCCTGATGCA 
CCCAATAGGG AAGAGGTATT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAGG 
CTTGGGGCCA CAGCAGAGAA GGCGGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CCTCAGTGAA GACAGCCCGA GAACTTACAC CCCAGGTGGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TCGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGACACT 
AAATCTCTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ACCTGGACAA GTGTAAAGTT 
GCCATGGCCA ACATTCAGCC TCAGATGCTG GTCGCCGGGG CAACCAGCAT TGCTCGTCGG 
GCCAACCGAA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAATTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAA TTGAGCAAAA CCATCTCCCC AATGGTGGTG 
GATGCAAAGG CCGTGGCAGG AAACATTTCT GACCCTGGAC TGCAAAAGAG CTTCCTGGAC 
TCAGGATACC GGATCCTAGG AGCTGTGGCC AAGGTCAGAG AAGCCTTCCA ACCACAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAACAGCTCC GACTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGTGAG GTCCCTCCAC CCAGGCCCCC ACCACCGGAG 
GAGAAGGATG AAGAATTCCC TGAGCAGAAA GCCGGGGAGG TGATTAACCA GCCAATGATG 
ATGGCTGCCA GGCAGCTCCA CGATGAAGCT CGCAAGTGGT CCAGCAAGCC GGGTAACCCA 
GCCGCTAAGG TGGGTATAAG TGTTGTAGCT GAGGCAGAGG CGGCTGATGC TGTTGGGTTC 
CCTGTCCCCC CTGACATGGA AGACGATTAC GAACCTGAGC TGCTGTTAAT GCCATCCAAT 
CAGCCGGTCA ACCAGCCCAT TCTGGCCGCG GCTCAGTCAT TGCATCGGGA AGCTACCAAG 
TGGTCTAGTA AGGTCATGAA ATCCTGGAAT GATCCCAGGT CTGCAGTGGA AGAGGTGCAA 
CCTTGTCTAA CATACTCCTG TTCTCATGAG TTCTTCCCAA ACTGTGTTCT CTCTGGCCTC 
GACGACTGCC TGGGAGGTTA CGCACCCCCT CGTGTAAATG ATCAGCCACT TCCTGGTGCC 
TGTGGATGCC TCTCACAGAG CACCCTGCCG TGGCATGGTT TGTGTATGCC TCACTGCATT 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( NM_001191370.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178299.1 Bos taurus vinculin (VCL), mRNA.

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CloneID	OBa37453
Clone ID Related Accession (Same CDS sequence)	NM_001191370.1
Accession Version	NM_001191370.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	vinculin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000028.1. On Jul 17, 2010 this sequence version replaced XM_001790292.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571259.38444.1, SRR5721694.7827.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145419 [ECO:0000348] ##Evidence-Data-END##

ATGCCGGTGT TTCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAATG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGATCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCTCC CCAGGTGATG CTGGTGAACA GGCCATCAGG 
CAGATTTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTGGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAAGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGA CAAGGTAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAGGTGGC CACAGCCCTA CAGAACTTAC AGACTAAAAC AAACAGGGCC 
GTAGCCAACA GCAGACCTGC CAAAGCAGCG GTCCACCTTG AAGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC TACTGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGA 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAGGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGCCC TCAGGCGAGA GCTCTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACGA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACAG CAGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCCCGAGAA CTTACACCCC AGGTGGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGACACTAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTTGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTC GCCGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGAATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAATTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGGTGGAT 
GCAAAGGCCG TGGCAGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTAGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC ACAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTCCGAC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCACC ACCGGAGGAG 
AAGGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCTCGC AAGTGGTCCA GCAAGCCGGG TAACCCAGCC 
GCTAAGGTGG GTATAAGTGT TGTAGCTGAG GCAGAGGCGG CTGATGCTGT TGGGTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGTT GATGGCTGAG 
ATGTCTCGAC TGGTCAGAGG GGGCAGCGGC ACCAAGCGGG CACTGATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAACCTTTTA CAGGTATGCG AGCGAATCCC AACCATAAGC 
ACCCAGCTCA AAATCCTGTC CACGGTGAAG GCCACCATGC TGGGTCGGAC CAACATAAGC 
GACGAGGAGT CCGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTTCG AGAAGCAGAA GCAGCTTCAA TCAAAATTCG AACAGATGCT 
GGGTTTACTC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178299.1
CDS	61..3465
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVVDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGNPAAKVGISVVAEAEAADAVGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	NM_001191370.1
Organism	Bos taurus(cattle)
Definition	Bos taurus vinculin (VCL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191370.1

ORF Insert Sequence:

ATGCCGGTGT TTCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAATG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGATCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCTCC CCAGGTGATG CTGGTGAACA GGCCATCAGG 
CAGATTTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTGGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAAGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGA CAAGGTAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAGGTGGC CACAGCCCTA CAGAACTTAC AGACTAAAAC AAACAGGGCC 
GTAGCCAACA GCAGACCTGC CAAAGCAGCG GTCCACCTTG AAGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC TACTGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGA 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAGGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGCCC TCAGGCGAGA GCTCTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACGA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACAG CAGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCCCGAGAA CTTACACCCC AGGTGGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGACACTAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTTGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTC GCCGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGAATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAATTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGGTGGAT 
GCAAAGGCCG TGGCAGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTAGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC ACAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTCCGAC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCACC ACCGGAGGAG 
AAGGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCTCGC AAGTGGTCCA GCAAGCCGGG TAACCCAGCC 
GCTAAGGTGG GTATAAGTGT TGTAGCTGAG GCAGAGGCGG CTGATGCTGT TGGGTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGTT GATGGCTGAG 
ATGTCTCGAC TGGTCAGAGG GGGCAGCGGC ACCAAGCGGG CACTGATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAACCTTTTA CAGGTATGCG AGCGAATCCC AACCATAAGC 
ACCCAGCTCA AAATCCTGTC CACGGTGAAG GCCACCATGC TGGGTCGGAC CAACATAAGC 
GACGAGGAGT CCGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTTCG AGAAGCAGAA GCAGCTTCAA TCAAAATTCG AACAGATGCT 
GGGTTTACTC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_005226481.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226538.1 Bos taurus vinculin (VCL), transcript variant X1, mRNA.

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CloneID	OBa34284
Clone ID Related Accession (Same CDS sequence)	XM_005226481.2
Accession Version	XM_005226481.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	vinculin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAATG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGATCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCTAAAGGTT GGCTCCGTGA TCCCAATGCC TCCCCAGGTG ATGCTGGTGA ACAGGCCATC 
AGGCAGATTT TAGATGAAGC TGGAAAAGTT GGTGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCCTGG GAACCTGCAA AATGCTGGGG CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGAGCCTC ACCGGTGGCC ATGCAGAAAG CCCAGCAAGT GTCTCAGGGT 
CTGGATGTGC TCACAGCAAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGAGAT GACATTCTGC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGG AGATCTACGA AGACAAGGTA AAGGAGATTC CCCAGAGGCC 
CGAGCATTAG CCAAACAGGT GGCCACAGCC CTACAGAACT TACAGACTAA AACAAACAGG 
GCCGTAGCCA ACAGCAGACC TGCCAAAGCA GCGGTCCACC TTGAAGGCAA GATTGAGCAA 
GCGCAGCGGT GGATTGATAA TCCTACTGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGAGGGCTTG TGGCCGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAG 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG CCCTCAGGCG AGAGCTCTTG CATCTCAGCT CCAAGACTCC 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGTGATACCA CGACTCCCAT CAAGCTGTTG GCAGTGGCAG CCACTGCTCC TCCTGATGCA 
CCCAATAGGG AAGAGGTATT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAGG 
CTTGGGGCCA CAGCAGAGAA GGCGGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CCTCAGTGAA GACAGCCCGA GAACTTACAC CCCAGGTGGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TCGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGACACT 
AAATCTCTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ACCTGGACAA GTGTAAAGTT 
GCCATGGCCA ACATTCAGCC TCAGATGCTG GTCGCCGGGG CAACCAGCAT TGCTCGTCGG 
GCCAACCGAA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAATTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAA TTGAGCAAAA CCATCTCCCC AATGGTGGTG 
GATGCAAAGG CCGTGGCAGG AAACATTTCT GACCCTGGAC TGCAAAAGAG CTTCCTGGAC 
TCAGGATACC GGATCCTAGG AGCTGTGGCC AAGGTCAGAG AAGCCTTCCA ACCACAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAACAGCTCC GACTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGTGAG GTCCCTCCAC CCAGGCCCCC ACCACCGGAG 
GAGAAGGATG AAGAATTCCC TGAGCAGAAA GCCGGGGAGG TGATTAACCA GCCAATGATG 
ATGGCTGCCA GGCAGCTCCA CGATGAAGCT CGCAAGTGGT CCAGCAAGCC GGGTAACCCA 
GCCGCTAAGG TGGGTATAAG TGTTGTAGCT GAGGCAGAGG CGGCTGATGC TGTTGGGTTC 
CCTGTCCCCC CTGACATGGA AGACGATTAC GAACCTGAGC TGCTGTTAAT GCCATCCAAT 
CAGCCGGTCA ACCAGCCCAT TCTGGCCGCG GCTCAGTCAT TGCATCGGGA AGCTACCAAG 
TGGTCTAGTA AGGGCAATGA CATCATTGCA GCAGCCAAGC GCATGGCTCT GTTGATGGCT 
GAGATGTCTC GACTGGTCAG AGGGGGCAGC GGCACCAAGC GGGCACTGAT TCAGTGTGCC 
AAGGACATCG CCAAGGCCTC AGATGAGGTG ACTCGGTTGG CCAAGGAGGT TGCCAAGCAG 
TGCACAGATA AGCGGATTAG AACCAACCTT TTACAGGTAT GCGAGCGAAT CCCAACCATA 
AGCACCCAGC TCAAAATCCT GTCCACGGTG AAGGCCACCA TGCTGGGTCG GACCAACATA 
AGCGACGAGG AGTCCGAGCA GGCCACAGAG ATGCTGGTTC ACAATGCCCA GAACCTCATG 
CAGTCTGTGA AGGAGACTGT TCGAGAAGCA GAAGCAGCTT CAATCAAAAT TCGAACAGAT 
GCTGGGTTTA CTCTGCGCTG GGTTAGAAAG ACTCCCTGGT ACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226538.1
CDS	116..3523
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVVDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGNPAAKVGISVVAEAEAADAVGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_005226481.2
Organism	Bos taurus(cattle)
Definition	Bos taurus vinculin (VCL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226481.2

ORF Insert Sequence:

ATGCCGGTGT TTCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAATG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGATCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCTAAAGGTT GGCTCCGTGA TCCCAATGCC TCCCCAGGTG ATGCTGGTGA ACAGGCCATC 
AGGCAGATTT TAGATGAAGC TGGAAAAGTT GGTGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCCTGG GAACCTGCAA AATGCTGGGG CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGAGCCTC ACCGGTGGCC ATGCAGAAAG CCCAGCAAGT GTCTCAGGGT 
CTGGATGTGC TCACAGCAAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGAGAT GACATTCTGC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGG AGATCTACGA AGACAAGGTA AAGGAGATTC CCCAGAGGCC 
CGAGCATTAG CCAAACAGGT GGCCACAGCC CTACAGAACT TACAGACTAA AACAAACAGG 
GCCGTAGCCA ACAGCAGACC TGCCAAAGCA GCGGTCCACC TTGAAGGCAA GATTGAGCAA 
GCGCAGCGGT GGATTGATAA TCCTACTGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGAGGGCTTG TGGCCGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAG 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG CCCTCAGGCG AGAGCTCTTG CATCTCAGCT CCAAGACTCC 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGTGATACCA CGACTCCCAT CAAGCTGTTG GCAGTGGCAG CCACTGCTCC TCCTGATGCA 
CCCAATAGGG AAGAGGTATT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAGG 
CTTGGGGCCA CAGCAGAGAA GGCGGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CCTCAGTGAA GACAGCCCGA GAACTTACAC CCCAGGTGGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TCGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGACACT 
AAATCTCTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ACCTGGACAA GTGTAAAGTT 
GCCATGGCCA ACATTCAGCC TCAGATGCTG GTCGCCGGGG CAACCAGCAT TGCTCGTCGG 
GCCAACCGAA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAATTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAA TTGAGCAAAA CCATCTCCCC AATGGTGGTG 
GATGCAAAGG CCGTGGCAGG AAACATTTCT GACCCTGGAC TGCAAAAGAG CTTCCTGGAC 
TCAGGATACC GGATCCTAGG AGCTGTGGCC AAGGTCAGAG AAGCCTTCCA ACCACAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAACAGCTCC GACTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGTGAG GTCCCTCCAC CCAGGCCCCC ACCACCGGAG 
GAGAAGGATG AAGAATTCCC TGAGCAGAAA GCCGGGGAGG TGATTAACCA GCCAATGATG 
ATGGCTGCCA GGCAGCTCCA CGATGAAGCT CGCAAGTGGT CCAGCAAGCC GGGTAACCCA 
GCCGCTAAGG TGGGTATAAG TGTTGTAGCT GAGGCAGAGG CGGCTGATGC TGTTGGGTTC 
CCTGTCCCCC CTGACATGGA AGACGATTAC GAACCTGAGC TGCTGTTAAT GCCATCCAAT 
CAGCCGGTCA ACCAGCCCAT TCTGGCCGCG GCTCAGTCAT TGCATCGGGA AGCTACCAAG 
TGGTCTAGTA AGGGCAATGA CATCATTGCA GCAGCCAAGC GCATGGCTCT GTTGATGGCT 
GAGATGTCTC GACTGGTCAG AGGGGGCAGC GGCACCAAGC GGGCACTGAT TCAGTGTGCC 
AAGGACATCG CCAAGGCCTC AGATGAGGTG ACTCGGTTGG CCAAGGAGGT TGCCAAGCAG 
TGCACAGATA AGCGGATTAG AACCAACCTT TTACAGGTAT GCGAGCGAAT CCCAACCATA 
AGCACCCAGC TCAAAATCCT GTCCACGGTG AAGGCCACCA TGCTGGGTCG GACCAACATA 
AGCGACGAGG AGTCCGAGCA GGCCACAGAG ATGCTGGTTC ACAATGCCCA GAACCTCATG 
CAGTCTGTGA AGGAGACTGT TCGAGAAGCA GAAGCAGCTT CAATCAAAAT TCGAACAGAT 
GCTGGGTTTA CTCTGCGCTG GGTTAGAAAG ACTCCCTGGT ACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_005226482.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226539.1 Bos taurus vinculin (VCL), transcript variant X2, mRNA.

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CloneID	OBa34285
Clone ID Related Accession (Same CDS sequence)	XM_005226482.2
Accession Version	XM_005226482.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	vinculin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226482.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAATG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGATCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCTAAAGGTT GGCTCCGTGA TCCCAATGCC TCCCCAGGTG ATGCTGGTGA ACAGGCCATC 
AGGCAGATTT TAGATGAAGC TGGAAAAGTT GGTGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCCTGG GAACCTGCAA AATGCTGGGG CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGAGCCTC ACCGGTGGCC ATGCAGAAAG CCCAGCAAGT GTCTCAGGGT 
CTGGATGTGC TCACAGCAAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGAGAT GACATTCTGC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGG AGATCTACGA AGACAAGGTA AAGGAGATTC CCCAGAGGCC 
CGAGCATTAG CCAAACAGGT GGCCACAGCC CTACAGAACT TACAGACTAA AACAAACAGG 
GCCGTAGCCA ACAGCAGACC TGCCAAAGCA GCGGTCCACC TTGAAGGCAA GATTGAGCAA 
GCGCAGCGGT GGATTGATAA TCCTACTGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGAGGGCTTG TGGCCGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAG 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG CCCTCAGGCG AGAGCTCTTG CATCTCAGCT CCAAGACTCC 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGTGATACCA CGACTCCCAT CAAGCTGTTG GCAGTGGCAG CCACTGCTCC TCCTGATGCA 
CCCAATAGGG AAGAGGTATT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAGG 
CTTGGGGCCA CAGCAGAGAA GGCGGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CCTCAGTGAA GACAGCCCGA GAACTTACAC CCCAGGTGGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TCGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGACACT 
AAATCTCTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ACCTGGACAA GTGTAAAGTT 
GCCATGGCCA ACATTCAGCC TCAGATGCTG GTCGCCGGGG CAACCAGCAT TGCTCGTCGG 
GCCAACCGAA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAATTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAA TTGAGCAAAA CCATCTCCCC AATGGTGGTG 
GATGCAAAGG CCGTGGCAGG AAACATTTCT GACCCTGGAC TGCAAAAGAG CTTCCTGGAC 
TCAGGATACC GGATCCTAGG AGCTGTGGCC AAGGTCAGAG AAGCCTTCCA ACCACAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAACAGCTCC GACTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGTGAG GTCCCTCCAC CCAGGCCCCC ACCACCGGAG 
GAGAAGGATG AAGAATTCCC TGAGCAGAAA GCCGGGGAGG TGATTAACCA GCCAATGATG 
ATGGCTGCCA GGCAGCTCCA CGATGAAGCT CGCAAGTGGT CCAGCAAGGG CAATGACATC 
ATTGCAGCAG CCAAGCGCAT GGCTCTGTTG ATGGCTGAGA TGTCTCGACT GGTCAGAGGG 
GGCAGCGGCA CCAAGCGGGC ACTGATTCAG TGTGCCAAGG ACATCGCCAA GGCCTCAGAT 
GAGGTGACTC GGTTGGCCAA GGAGGTTGCC AAGCAGTGCA CAGATAAGCG GATTAGAACC 
AACCTTTTAC AGGTATGCGA GCGAATCCCA ACCATAAGCA CCCAGCTCAA AATCCTGTCC 
ACGGTGAAGG CCACCATGCT GGGTCGGACC AACATAAGCG ACGAGGAGTC CGAGCAGGCC 
ACAGAGATGC TGGTTCACAA TGCCCAGAAC CTCATGCAGT CTGTGAAGGA GACTGTTCGA 
GAAGCAGAAG CAGCTTCAAT CAAAATTCGA ACAGATGCTG GGTTTACTCT GCGCTGGGTT 
AGAAAGACTC CCTGGTACCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226539.1
CDS	116..3319
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVVDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_005226482.2
Organism	Bos taurus(cattle)
Definition	Bos taurus vinculin (VCL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226482.2

ORF Insert Sequence:

ATGCCGGTGT TTCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAATG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGATCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCTAAAGGTT GGCTCCGTGA TCCCAATGCC TCCCCAGGTG ATGCTGGTGA ACAGGCCATC 
AGGCAGATTT TAGATGAAGC TGGAAAAGTT GGTGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCCTGG GAACCTGCAA AATGCTGGGG CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGAGCCTC ACCGGTGGCC ATGCAGAAAG CCCAGCAAGT GTCTCAGGGT 
CTGGATGTGC TCACAGCAAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGAGAT GACATTCTGC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGG AGATCTACGA AGACAAGGTA AAGGAGATTC CCCAGAGGCC 
CGAGCATTAG CCAAACAGGT GGCCACAGCC CTACAGAACT TACAGACTAA AACAAACAGG 
GCCGTAGCCA ACAGCAGACC TGCCAAAGCA GCGGTCCACC TTGAAGGCAA GATTGAGCAA 
GCGCAGCGGT GGATTGATAA TCCTACTGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGAGGGCTTG TGGCCGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAG 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG CCCTCAGGCG AGAGCTCTTG CATCTCAGCT CCAAGACTCC 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGTGATACCA CGACTCCCAT CAAGCTGTTG GCAGTGGCAG CCACTGCTCC TCCTGATGCA 
CCCAATAGGG AAGAGGTATT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAGG 
CTTGGGGCCA CAGCAGAGAA GGCGGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CCTCAGTGAA GACAGCCCGA GAACTTACAC CCCAGGTGGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TCGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGACACT 
AAATCTCTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ACCTGGACAA GTGTAAAGTT 
GCCATGGCCA ACATTCAGCC TCAGATGCTG GTCGCCGGGG CAACCAGCAT TGCTCGTCGG 
GCCAACCGAA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAATTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAA TTGAGCAAAA CCATCTCCCC AATGGTGGTG 
GATGCAAAGG CCGTGGCAGG AAACATTTCT GACCCTGGAC TGCAAAAGAG CTTCCTGGAC 
TCAGGATACC GGATCCTAGG AGCTGTGGCC AAGGTCAGAG AAGCCTTCCA ACCACAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAACAGCTCC GACTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGTGAG GTCCCTCCAC CCAGGCCCCC ACCACCGGAG 
GAGAAGGATG AAGAATTCCC TGAGCAGAAA GCCGGGGAGG TGATTAACCA GCCAATGATG 
ATGGCTGCCA GGCAGCTCCA CGATGAAGCT CGCAAGTGGT CCAGCAAGGG CAATGACATC 
ATTGCAGCAG CCAAGCGCAT GGCTCTGTTG ATGGCTGAGA TGTCTCGACT GGTCAGAGGG 
GGCAGCGGCA CCAAGCGGGC ACTGATTCAG TGTGCCAAGG ACATCGCCAA GGCCTCAGAT 
GAGGTGACTC GGTTGGCCAA GGAGGTTGCC AAGCAGTGCA CAGATAAGCG GATTAGAACC 
AACCTTTTAC AGGTATGCGA GCGAATCCCA ACCATAAGCA CCCAGCTCAA AATCCTGTCC 
ACGGTGAAGG CCACCATGCT GGGTCGGACC AACATAAGCG ACGAGGAGTC CGAGCAGGCC 
ACAGAGATGC TGGTTCACAA TGCCCAGAAC CTCATGCAGT CTGTGAAGGA GACTGTTCGA 
GAAGCAGAAG CAGCTTCAAT CAAAATTCGA ACAGATGCTG GGTTTACTCT GCGCTGGGTT 
AGAAAGACTC CCTGGTACCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_005226483.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226540.1 Bos taurus vinculin (VCL), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa34286
Clone ID Related Accession (Same CDS sequence)	XM_005226483.2 , XM_005226483.3
Accession Version	XM_005226483.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	vinculin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226483.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAATG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGATCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCTCC CCAGGTGATG CTGGTGAACA GGCCATCAGG 
CAGATTTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTGGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAAGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGA CAAGGTAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAGGTGGC CACAGCCCTA CAGAACTTAC AGACTAAAAC AAACAGGGCC 
GTAGCCAACA GCAGACCTGC CAAAGCAGCG GTCCACCTTG AAGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC TACTGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGA 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAGGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGCCC TCAGGCGAGA GCTCTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACGA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACAG CAGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCCCGAGAA CTTACACCCC AGGTGGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGACACTAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTTGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTC GCCGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGAATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAATTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGGTGGAT 
GCAAAGGCCG TGGCAGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTAGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC ACAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTCCGAC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCACC ACCGGAGGAG 
AAGGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCTCGC AAGTGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGTTGATG GCTGAGATGT CTCGACTGGT CAGAGGGGGC 
AGCGGCACCA AGCGGGCACT GATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAC 
CTTTTACAGG TATGCGAGCG AATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACG 
GTGAAGGCCA CCATGCTGGG TCGGACCAAC ATAAGCGACG AGGAGTCCGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTTCGAGAA 
GCAGAAGCAG CTTCAATCAA AATTCGAACA GATGCTGGGT TTACTCTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226540.1
CDS	1..3201
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVVDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_005226483.2
Organism	Bos taurus(cattle)
Definition	Bos taurus vinculin (VCL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226483.2

ORF Insert Sequence:

ATGCCGGTGT TTCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAATG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGATCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCTCC CCAGGTGATG CTGGTGAACA GGCCATCAGG 
CAGATTTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTGGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAAGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGA CAAGGTAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAGGTGGC CACAGCCCTA CAGAACTTAC AGACTAAAAC AAACAGGGCC 
GTAGCCAACA GCAGACCTGC CAAAGCAGCG GTCCACCTTG AAGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC TACTGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGA 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAGGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGCCC TCAGGCGAGA GCTCTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACGA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACAG CAGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCCCGAGAA CTTACACCCC AGGTGGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGACACTAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTTGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTC GCCGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGAATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAATTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGGTGGAT 
GCAAAGGCCG TGGCAGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTAGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC ACAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTCCGAC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCACC ACCGGAGGAG 
AAGGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCTCGC AAGTGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGTTGATG GCTGAGATGT CTCGACTGGT CAGAGGGGGC 
AGCGGCACCA AGCGGGCACT GATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAC 
CTTTTACAGG TATGCGAGCG AATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACG 
GTGAAGGCCA CCATGCTGGG TCGGACCAAC ATAAGCGACG AGGAGTCCGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTTCGAGAA 
GCAGAAGCAG CTTCAATCAA AATTCGAACA GATGCTGGGT TTACTCTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_005226483.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226540.1 Bos taurus vinculin (VCL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa34286
Clone ID Related Accession (Same CDS sequence)	XM_005226483.2 , XM_005226483.3
Accession Version	XM_005226483.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	vinculin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226483.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAATG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGATCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCTCC CCAGGTGATG CTGGTGAACA GGCCATCAGG 
CAGATTTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTGGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAAGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGA CAAGGTAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAGGTGGC CACAGCCCTA CAGAACTTAC AGACTAAAAC AAACAGGGCC 
GTAGCCAACA GCAGACCTGC CAAAGCAGCG GTCCACCTTG AAGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC TACTGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGA 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAGGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGCCC TCAGGCGAGA GCTCTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACGA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACAG CAGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCCCGAGAA CTTACACCCC AGGTGGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGACACTAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTTGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTC GCCGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGAATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAATTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGGTGGAT 
GCAAAGGCCG TGGCAGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTAGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC ACAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTCCGAC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCACC ACCGGAGGAG 
AAGGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCTCGC AAGTGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGTTGATG GCTGAGATGT CTCGACTGGT CAGAGGGGGC 
AGCGGCACCA AGCGGGCACT GATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAC 
CTTTTACAGG TATGCGAGCG AATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACG 
GTGAAGGCCA CCATGCTGGG TCGGACCAAC ATAAGCGACG AGGAGTCCGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTTCGAGAA 
GCAGAAGCAG CTTCAATCAA AATTCGAACA GATGCTGGGT TTACTCTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226540.1
CDS	143..3343
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVVDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_005226483.3
Organism	Bos taurus(cattle)
Definition	Bos taurus vinculin (VCL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226483.3

ORF Insert Sequence:

ATGCCGGTGT TTCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAATG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGATCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCTCC CCAGGTGATG CTGGTGAACA GGCCATCAGG 
CAGATTTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTGGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAAGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGA CAAGGTAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAGGTGGC CACAGCCCTA CAGAACTTAC AGACTAAAAC AAACAGGGCC 
GTAGCCAACA GCAGACCTGC CAAAGCAGCG GTCCACCTTG AAGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC TACTGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGA 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAGGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGCCC TCAGGCGAGA GCTCTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACGA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACAG CAGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCCCGAGAA CTTACACCCC AGGTGGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGACACTAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTTGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTC GCCGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGAATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAATTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGGTGGAT 
GCAAAGGCCG TGGCAGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTAGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC ACAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTCCGAC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCACC ACCGGAGGAG 
AAGGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCTCGC AAGTGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGTTGATG GCTGAGATGT CTCGACTGGT CAGAGGGGGC 
AGCGGCACCA AGCGGGCACT GATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAC 
CTTTTACAGG TATGCGAGCG AATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACG 
GTGAAGGCCA CCATGCTGGG TCGGACCAAC ATAAGCGACG AGGAGTCCGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTTCGAGAA 
GCAGAAGCAG CTTCAATCAA AATTCGAACA GATGCTGGGT TTACTCTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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VCL cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

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