TLN1 cDNA ORF clone, Bos taurus(Cattle)

The following TLN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa216244 NM_001205428.2
Latest version!
Bos taurus talin 1 (TLN1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa237296 NM_001205428.3
Latest version!
Bos taurus talin 1 (TLN1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa237295 XM_010807953.2
Latest version!
Bos taurus talin 1 (TLN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa237295 XM_005210126.3
Latest version!
Bos taurus talin 1 (TLN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa237296 XM_024995995.1
Latest version!
Bos taurus talin 1 (TLN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa41129 NM_001205428.1
Latest version!
Bos taurus talin 1 (TLN1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa22069 XM_010807953.1
Latest version!
Bos taurus talin 1 (TLN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa22069 XM_005210126.2
Latest version!
Bos taurus talin 1 (TLN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa216244
    Clone ID Related Accession (Same CDS sequence) NM_001205428.2
    Accession Version NM_001205428.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product talin-1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02023131.1. On Jun 30, 2017 this sequence version replaced NM_001205428.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTTGCAC TTTCGCTGAA GATCAGCATT GGGAATGTGG TGAAGACGAT GCAATTTGAG 
    CCGTCTACCA TGGTGTATGA CGCCTGTCGG ATCATCCGTG AGCGGATCCC AGAGGCCCTA
    TCTGGCCCTC CCAGCGACTT TGGGCTGTTT CTGTCAGATG ATGACCCCAA AAAGGGTATA
    TGGCTGGAGG CAGGGAAGGC TTTGGATTAT TACATGCTCC GAAACGGGGA CACCATGGAG
    TATAGGAAGA AACAGAGGCC CTTGAAGATC CGCATGCTGG ACGGCACCGT GAAGACTGTC
    ATGGTGGACG ACTCGAAGAC TGTCACGGAC ATGCTCATGA CCATCTGTGC CCGCATTGGC
    ATCACCAACC ATGATGAATA TTCACTGGTT CGAGAGATTA TGGAAGAGAA GAAGGAGGAG
    GTGACAGGGA CCTTACGAAA GGACAAGACA CTGCTGCGAG ACGAAAAGAA GATGGAGAAA
    CTGAAGCAGA AGTTGCACAC AGATGACGAG TTGAACTGGC TGGACCATGG CCGGACCCTG
    AGGGAGCAGG GAGTGGAGGA GCACGAAACG CTTCTGCTCC GGAGGAAGTT CTTCTACTCA
    GATCAGAACG TGGACTCCCG GGACCCCGTC CAGCTGAACC TTCTCTACGT GCAGGCACGA
    GATGACATCC TGAATGGCTC CCATCCTGTC TCCTTCGACA AGGCCTGTGA GTTTGCTGGC
    TTCCAATGCC AGATCCAGTT TGGGCCCCAC AATGAGCAGA AGCACAAGGC CGGCTTCCTC
    GACCTGAAGG ACTTCCTGCC CAAGGAGTAT GTGAAGCAGA AGGGAGAGCG TAAGATCTTC
    CAGGCACACA AGAACTGTGG ACAGATGAGT GAGATTGAGG CCAAGGTACG CTACGTGAAG
    CTAGCCCGTT CCCTCAAGAC GTACGGTGTC TCCTTCTTCC TGGTCAAGGA AAAGATGAAA
    GGAAAGAACA AGTTGGTGCC CAGGCTTCTG GGCATCACTA AGGAGTGTGT GATGCGAGTG
    GACGAGAAGA CCAAGGAGGT GATCCAGGAA TGGAACCTCA CCAACATAAA ACGCTGGGCT
    GCGTCTCCCA AGAGCTTCAC CCTGGATTTC GGAGACTACC AAGATGGCTA CTACTCGGTG
    CAGACGACCG AGGGGGAGCA GATCGCTCAG CTCATCGCTG GCTACATCGA CATCATCCTT
    AAGAAGAAAA AAAGCAAGGA TCATTTTGGG CTGGAGGGAG ACGAGGAGTC TACTATGCTG
    GAGGACTCCG TGTCCCCCAA AAAGTCCACT GTGCTTCAGC AGCAGTATAA CCGGGTGGGG
    AAGGTGGAGC ATGGCTCCGT GGCCCTGCCC GCCATCATGC GCTCCGGAGC CTCGGGTCCC
    GAGAATTTCC AGGTGGGCAG CATGCCACCT GCCCAGCAGC AGATCACCAG TGGCCAGATG
    CACCGAGGAC ACATGCCTCC TTTGACGTCA GCCCAGCAGG CGCTCACTGG GACCATCAAC
    TCCAGCATGC AGGCTGTGCA AGCTGCCCAG GCCACACTGG ATGACTTTGA CACTCTGCCC
    CCTCTGGGCC AGGACGCTGC CTCAAAGGCC TGGCGTAAGA ACAAGATGGA TGAATCCAAG
    CATGAAATCC ACTCCCAGGT AGACGCCATC ACCGCTGGTA CCGCCTCTGT GGTGAACCTC
    ACAGCAGGGG ACCCTGCTGA GACAGACTAC ACGGCAGTGG GCTGTGCGGT CACCACCATC
    TCCTCCAACC TGACGGAGAT GTCCCGTGGC GTGAAGCTGC TTGCTGCCCT GCTGGAGGAT
    GAAGGCGGCA GCGGCCGGCC CCTGCTGCAG GCGGCCAAGG GCCTGGCGGG GGCGGTGTCA
    GAGCTGCTGC GCAGTGCCCA GCCAGCCAGT GCCGAGCCCC GTCAGAACCT GCTGCAAGCA
    GCTGGGAACG TGGGCCAGGC CAGTGGGGAG CTGTTGCAAC AAATTGGGGA AAGTGATACT
    GACCCCCACT TCCAGGACGT GCTCATGCAG CTAGCCAAGG CCGTGGCAAG CGCTGCAGCT
    GCCCTGGTCC TCAAGGCCAA GAGTGTGGCC CAGCGAACAG AGGACTCAGG GCTTCAGACC
    CAGGTCATCG CTGCGGCAAC ACAGTGTGCC CTGTCCACCT CCCAGCTGGT GGCCTGTACC
    AAGGTGGTGG CTCCTACTAT CAGCTCCCCA GTCTGCCAAG AACAGCTGGT AGAGGCTGGG
    CGACTAGTGG CCAAGGCTGT GGAGGGCTGT GTGTCCGCTT CCCAGGCCGC CACAGAGGAT
    GCCCAGCTGT TACGGGGGGT AGGTGCTGCG GCCACAGCTG TCACCCAGGC CCTGAATGAG
    CTGCTGCAGC ACGTGAGGGC CCATGCCACA GGGGCTGGGC CTGCTGGCCG CTACGACCAG
    GCCACTGACA CCATCCTCAC TGTCACCTAT TTCATCTTCA GCTCCATGGG TGATGCTGGT
    GAGATGGTGC GACAGGCCCG CATCCTCGCC CAAGCCACCT CTGACCTGGT GAACGCCATC
    AAGGCTGATG CCGAAGGGGA GAGTGATCTG GAGAATTCCC GCAAGCTGTT AAGTGCCGCC
    AAGATCCTGG CCGATGCCAC AGCCAAGATG GTGGAGGCTG CCAAGGGAGC AGCCGCCAAT
    CCTGACAGTG AGGAGCAACA GCAGCGGCTG CGGGAGGCAG CAGAGGGTCT CCGCATGGCA
    ACCAATGCCG CTGCACAGAA CGCCATCAAG AAAAAGCTGG TGCAGCGCCT TGAGCATGCA
    GCCAAACAGG CTGCAGCCTC GGCTACACAG ACTATCGCAG CAGCCCAACA TGCAACCTCC
    ACTCCCAAGG CCTCTGCTGG CCCCCAGCCC CTGCTGGTGC AGAGCTGCAA GGCTGTGGCA
    GAGCAGATTC CACTGCTGGT GCAGGGCGTC CGAGGGAGCC AAGCGCAGCC TGACAGCCCC
    AGTGCTCAGC TAGCCCTCAT CGCTGCCAGC CAAAGCTTCC TGCAGCCAGG TGGGAAGATG
    GTGGCAGCCG CAAAGGCCTC TGTGCCAACA ATTCAGGACC AAGCATCGGC CATGCAGCTG
    AGTCAGTGTG CTAAAAACCT TGGCACCGCG TTGGCTGAAC TCCGTACTGC TGCCCAGAAG
    GCTCAGGAAG CGTGTGGGCC TTTGGAAATG GATTCTGCTC TGAGTGTGGT GCAGAATCTG
    GAGAGAGACC TGCAGGAAGT GAAGGCAGCT GCTCGAGACG GCAAGCTGAA GCCCTTGCCT
    GGGGAGACGA TGGAGAAATG TGCCCAGGAC CTGGGCAACA GCACCAAAGC CGTGAGCTCC
    GCTATTGCCC AGCTGCTGGG GGAGGTCGCC CAGGGCAATG AGAATTACGC AGGCATTGCA
    GCTCGGGATG TGGCAGGTGG GCTGCGGTCA CTGGCCCAAG CTGCTAGGGG CGTAGCTGCC
    CTGACATCAG ATCCTGCAGT GCAAGCCATT GTGCTCGACA CAGCCAGCGA TGTCCTGGAC
    AAGGCCAGCA GCCTCATCGA GGAGGCAAAG AAAGCAGCCG GCCATCCAGG GGACCCTGAG
    AGCCAGCAGC GGCTTGCCCA GGTGGCTAAA GCTGTGACCC AGGCCCTGAA CCGTTGCGTC
    AGCTGCCTGC CTGGCCAGCG TGATGTGGAC AATGCCCTGA GGGCAGTGGG AGATGCCAGC
    AAGCGACTCC TGAGTGACTC GCTTCCCCCC AGCACTGGGA CCTTTCAGGA GGCTCAGAGC
    CGGCTGAATG AAGCCGCTGC CGGGCTGAAT CAGGCAGCCA CAGAGCTGGT GCAGGCCTCT
    CGGGGAACCC CTCAGGACCT GGCCCGAGCC TCAGGTCGAT TTGGACAAGA CTTCAGCACC
    TTCCTGGAAG CCGGCGTGGA GATGGCAGGC CAGGCTCCGA GCCAGGAGGA CCGTGCCCAG
    GTCGTATCCA ACTTGAAGGG CATCTCCATG TCTTCAAGCA AACTTCTTCT CGCTGCCAAG
    GCCCTGTCCA CAGACCCTGC AGCCCCAACC CTCAAGAGTC AGCTGGCTGC AGCTGCCCGG
    GCAGTGACCG ACAGCATCAA CCAGCTCATC AGCATGTGCA CCCAGCAGGC GCCAGGCCAG
    AAGGAGTGTG ATAATGCCCT GCGGGAATTG GAGACGGTCC GGGAACTCCT AGAGAACCCA
    GTCCAGCCCA TCAATGACAT GTCCTACTTT GGCTGTCTGG ACAGCGTCAT GGAAAACTCA
    AAGGTGCTGG GTGAGGCCAT GACCGGCATC TCCCAAAATG CCAAGAACGG AAACTTGCCG
    GAGTTTGGGG AGGCTGTCGC CACAGCCTCC AAGGCCCTCT GCGGCTTCAC CGAGGCAGCT
    GCACAGGCTG CATATCTGGT TGGCGTGTCT GACCCCAACA GCCAAGCTGG ACAACAAGGG
    CTGGTGGAGC CCACACAGTT TGCCCGAGCA AACCAGGCAA TTCAGATGGC CTGTCAGAGT
    TTGGGGGAGC CTGGCTGCAC CCAGGCCCAG GTGCTCTCTG CAGCCACCAT TGTGGCCAAA
    CACACGTCTG CGCTGTGTAA CAGCTGCCGC CTGGCTTCTG CCCGAACCGC CAATCCCACT
    GCCAAGCGCC AGTTCGTACA GTCAGCCAAG GAGGTGGCCA ACAGCACGGC CAACCTTGTC
    AAGACCATCA AGGCGCTGGA TGGGGCCTTC ACAGAGGAGA ACCGTGCCCA GTGCCGAGCA
    GCAACGGCCC CTTTGCTGGA GGCTGTGGAC AATCTGAGCG CCTTTGCGTC CAACCCTGAG
    TTCTCCAGTG TTCCTGCCCA GATCAGCCCT GAGGGCCGGG CTGCCATGGA GCCCATTGTG
    ATCTCTGCTA AGACAATGTT GGAGAGTGCT GGGGGCCTTA TTCAGACAGC CCGAGCCCTG
    GCGGTCAATC CGCGGGACCC ACCACGCTGG TCCGTGCTGG CTGGCCACTC CCGTACTGTC
    TCAGACTCCA TCAAGAAGCT GATCACAAGC ATGAGGGACA AGGCCCCAGG GCAGTTGGAG
    TGTGAGACAG CCATCGCAGC TCTGAACAGC TGTCTGCGGG ACCTTGACCA GGCTTCTCTT
    GCTGCAGTCA GCCAGCAGCT CGCTCCCCGT GAGGGGATCT CTCAGGAGGC CTTGCACACC
    CAGATGCTCA CTGCGGTGCA GGAAATCTCC CATCTTATTG AGCCGCTGGC CAGCGCTGCC
    CGGGCTGAAG CCTCCCAGCT GGGACACAAG GTGTCCCAGA TGGCCCAGTA CTTTGAGCCG
    CTCACTCTGG CTGCCGTGGG TGCTGCCTCC AAAACCCTGA GCCACCCACA GCAGATGGCG
    CTCCTGGACC AGACCAAAAC CTTGGCCGAG TCTGCCCTGC AGCTGCTATA CACAGCCAAG
    GAAGCTGGCG GTAACCCCAA GCAAGCAGCT CACACCCAGG AAGCCCTGGA GGAGGCTGTG
    CAGATGATGA CAGAGGCCGT AGAGGACCTG ACAACCACCC TCAACGAGGC AGCCAGTGCT
    GCCGGGGTCG TCGGTGGCAT GGTGGACTCC ATCACCCAGG CCATCAACCA GCTAGATGAA
    GGACCAATGG GTGAACCAGA GGGTTCCTTC GTGGATTACC AGACCACCAT GGTGCGGACA
    GCCAAGGCCA TTGCTGTCAC CGTTCAGGAG ATGGTAACCA AGTCAAACAC CAGCCCTGAG
    GAGCTGGGCC CTCTCGCTAA CCAGCTGACT AGTGACTATG GGCGTCTTGC CTCCCAGGCC
    AAGCCTGCAG CTGTGGCTGC TGAAAATGAA GAGATAGGCG CCCACATCAA GCACCGGGTT
    CAGGAGCTGG GGCACGGCTG CGCAGCTCTG GTCACCAAGG CAGGCGCCCT GCAGTGTAGC
    CCTAGTGATG CCTACACCAA GAAGGAGCTC ATAGAGTGTG CCCGGAGAGT CTCTGAGAAG
    GTCTCCCACG TGCTGGCTGC GCTCCAGGCT GGGAATCGTG GCACCCAGGC CTGCATCACA
    GCCGCCAGCG CTGTGTCCGG CATCATCGCC GACCTGGACA CCACCATCAT GTTCGCCACT
    GCCGGCACAC TCAACCGTGA GGGTGCCGAA ACCTTTGCTG ACCACCGAGA GGGCATTCTG
    AAGACTGCCA AGGTGCTGGT GGAAGACACC AAGGTCCTGG TGCAGAATGC AGCTGGGAGC
    CAGGAGAAGC TGGCACAGGC AGCCCAGTCC TCCGTGGCCA CCATCACCCG CCTCGCCGAT
    GTGGTCAAGC TGGGTGCAGC CAGCCTGGGA GCTGAGGACC CAGAGACCCA GGTGGTGCTG
    ATCAATGCAG TGAAAGATGT GGCCAAGGCC CTGGGAGACC TCATCAGTGC CACAAAGGCT
    GCGGCTGGCA AAGTCGGCGA CGACCCTGCT GTGTGGCAGC TCAAGAACTC TGCCAAGGTG
    ATGGTAACCA ATGTGACATC ACTGCTCAAG ACCGTGAAAG CGGTAGAGGA TGAGGCCACC
    AAAGGCACGC GCGCCCTGGA GGCCACAACG GAGCATATTC GGCAGGAGCT GGCGGTCTTC
    TGCTCCCCAG AGCCACCTGC CAAGACCTCG ACCCCTGAAG ATTTCATCCG CATGACCAAG
    GGTATCACCA TGGCAACCGC CAAGGCCGTT GCCGCTGGGA ACTCCTGTCG CCAGGAGGAT
    GTCATTGCCA CAGCCAATCT GAGTCGCCGT GCTATTGCAG ATATGCTCCG GGCTTGCAAG
    GAAGCTGCCT ACCACCCAGA AGTGGCTCCA GACGTGCGGC TCCGAGCCCT ACACTTTGGC
    CGGGAATGTG CCAATGGCTA CCTGGAACTG CTGGACCACG TGCTGCTGAC CCTGCAGAAG
    CCAAGCCCAG AATTGAAGCA GCAATTGACG GTGCACTCAA AGCGTGTGGC TGGATCAGTC
    ACTGAGCTCA TCCAGGCTGC TGAGGCCATG AAGGGAACAG AATGGGTGGA CCCAGAGGAC
    CCCACAGTCA TTGCTGAGAA TGAACTCCTG GGAGCCGCGG CTGCCATTGA GGCTGCGGCC
    AAGAAGCTGG AGCAGCTGAA GCCCCGGGCC AAACCTAAGG AGGCGGACGA GTCCTTGAAC
    TTTGAGGAGC AGATACTGGA AGCTGCCAAG TCCATCGCAG CAGCCACCAG CGCCCTGGTG
    AAGGCTGCCT CAGCTGCACA GAGGGAACTG GTGGCCCAGG GGAAGGTGGG CGCCATTCCA
    GCCAACGCAC TGGATGATGG ACAGTGGTCC CAGGGCCTCA TCTCTGCTGC CCGGATGGTG
    GCTGCGGCCA CCAACAACCT GTGTGAGGCA GCCAATGCAG CTGTCCAAGG CCACGCCAGC
    CAGGAGAAGC TCATCTCATC AGCCAAGCAG GTCGCCGCCT CCACAGCCCA GCTCCTCGTA
    GCCTGCAAGG TCAAGGCCGA CCAGGACTCT GAGGCAATGA AGCGGCTTCA GGCTGCCGGC
    AACGCAGTGA AGCGAGCCTC AGACAACCTG GTGAAGGCAG CCCAGAAGGC TGCAGCCTTT
    GAAGAACAGG AGAATGAGAC CGTGGTGGTG AAGGAGAAGA TGGTCGGGGG CATTGCCCAG
    ATCATTGCTG CCCAGGAAGA GATGCTTCGG AAGGAACGAG AGCTGGAGGA GGCACGGAAG
    AAGCTGGCGC AGATCCGGCA GCAGCAGTAC AAATTCTTAC CTTCAGAGCT TCGAGACGAG
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192357.2
    CDS319..7944
    Translation

    Target ORF information:

    RefSeq Version NM_001205428.2
    Organism Bos taurus(cattle)
    Definition Bos taurus talin 1 (TLN1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205428.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTTGCAC TTTCGCTGAA GATCAGCATT GGGAATGTGG TGAAGACGAT GCAATTTGAG 
    CCGTCTACCA TGGTGTATGA CGCCTGTCGG ATCATCCGTG AGCGGATCCC AGAGGCCCTA
    TCTGGCCCTC CCAGCGACTT TGGGCTGTTT CTGTCAGATG ATGACCCCAA AAAGGGTATA
    TGGCTGGAGG CAGGGAAGGC TTTGGATTAT TACATGCTCC GAAACGGGGA CACCATGGAG
    TATAGGAAGA AACAGAGGCC CTTGAAGATC CGCATGCTGG ACGGCACCGT GAAGACTGTC
    ATGGTGGACG ACTCGAAGAC TGTCACGGAC ATGCTCATGA CCATCTGTGC CCGCATTGGC
    ATCACCAACC ATGATGAATA TTCACTGGTT CGAGAGATTA TGGAAGAGAA GAAGGAGGAG
    GTGACAGGGA CCTTACGAAA GGACAAGACA CTGCTGCGAG ACGAAAAGAA GATGGAGAAA
    CTGAAGCAGA AGTTGCACAC AGATGACGAG TTGAACTGGC TGGACCATGG CCGGACCCTG
    AGGGAGCAGG GAGTGGAGGA GCACGAAACG CTTCTGCTCC GGAGGAAGTT CTTCTACTCA
    GATCAGAACG TGGACTCCCG GGACCCCGTC CAGCTGAACC TTCTCTACGT GCAGGCACGA
    GATGACATCC TGAATGGCTC CCATCCTGTC TCCTTCGACA AGGCCTGTGA GTTTGCTGGC
    TTCCAATGCC AGATCCAGTT TGGGCCCCAC AATGAGCAGA AGCACAAGGC CGGCTTCCTC
    GACCTGAAGG ACTTCCTGCC CAAGGAGTAT GTGAAGCAGA AGGGAGAGCG TAAGATCTTC
    CAGGCACACA AGAACTGTGG ACAGATGAGT GAGATTGAGG CCAAGGTACG CTACGTGAAG
    CTAGCCCGTT CCCTCAAGAC GTACGGTGTC TCCTTCTTCC TGGTCAAGGA AAAGATGAAA
    GGAAAGAACA AGTTGGTGCC CAGGCTTCTG GGCATCACTA AGGAGTGTGT GATGCGAGTG
    GACGAGAAGA CCAAGGAGGT GATCCAGGAA TGGAACCTCA CCAACATAAA ACGCTGGGCT
    GCGTCTCCCA AGAGCTTCAC CCTGGATTTC GGAGACTACC AAGATGGCTA CTACTCGGTG
    CAGACGACCG AGGGGGAGCA GATCGCTCAG CTCATCGCTG GCTACATCGA CATCATCCTT
    AAGAAGAAAA AAAGCAAGGA TCATTTTGGG CTGGAGGGAG ACGAGGAGTC TACTATGCTG
    GAGGACTCCG TGTCCCCCAA AAAGTCCACT GTGCTTCAGC AGCAGTATAA CCGGGTGGGG
    AAGGTGGAGC ATGGCTCCGT GGCCCTGCCC GCCATCATGC GCTCCGGAGC CTCGGGTCCC
    GAGAATTTCC AGGTGGGCAG CATGCCACCT GCCCAGCAGC AGATCACCAG TGGCCAGATG
    CACCGAGGAC ACATGCCTCC TTTGACGTCA GCCCAGCAGG CGCTCACTGG GACCATCAAC
    TCCAGCATGC AGGCTGTGCA AGCTGCCCAG GCCACACTGG ATGACTTTGA CACTCTGCCC
    CCTCTGGGCC AGGACGCTGC CTCAAAGGCC TGGCGTAAGA ACAAGATGGA TGAATCCAAG
    CATGAAATCC ACTCCCAGGT AGACGCCATC ACCGCTGGTA CCGCCTCTGT GGTGAACCTC
    ACAGCAGGGG ACCCTGCTGA GACAGACTAC ACGGCAGTGG GCTGTGCGGT CACCACCATC
    TCCTCCAACC TGACGGAGAT GTCCCGTGGC GTGAAGCTGC TTGCTGCCCT GCTGGAGGAT
    GAAGGCGGCA GCGGCCGGCC CCTGCTGCAG GCGGCCAAGG GCCTGGCGGG GGCGGTGTCA
    GAGCTGCTGC GCAGTGCCCA GCCAGCCAGT GCCGAGCCCC GTCAGAACCT GCTGCAAGCA
    GCTGGGAACG TGGGCCAGGC CAGTGGGGAG CTGTTGCAAC AAATTGGGGA AAGTGATACT
    GACCCCCACT TCCAGGACGT GCTCATGCAG CTAGCCAAGG CCGTGGCAAG CGCTGCAGCT
    GCCCTGGTCC TCAAGGCCAA GAGTGTGGCC CAGCGAACAG AGGACTCAGG GCTTCAGACC
    CAGGTCATCG CTGCGGCAAC ACAGTGTGCC CTGTCCACCT CCCAGCTGGT GGCCTGTACC
    AAGGTGGTGG CTCCTACTAT CAGCTCCCCA GTCTGCCAAG AACAGCTGGT AGAGGCTGGG
    CGACTAGTGG CCAAGGCTGT GGAGGGCTGT GTGTCCGCTT CCCAGGCCGC CACAGAGGAT
    GCCCAGCTGT TACGGGGGGT AGGTGCTGCG GCCACAGCTG TCACCCAGGC CCTGAATGAG
    CTGCTGCAGC ACGTGAGGGC CCATGCCACA GGGGCTGGGC CTGCTGGCCG CTACGACCAG
    GCCACTGACA CCATCCTCAC TGTCACCTAT TTCATCTTCA GCTCCATGGG TGATGCTGGT
    GAGATGGTGC GACAGGCCCG CATCCTCGCC CAAGCCACCT CTGACCTGGT GAACGCCATC
    AAGGCTGATG CCGAAGGGGA GAGTGATCTG GAGAATTCCC GCAAGCTGTT AAGTGCCGCC
    AAGATCCTGG CCGATGCCAC AGCCAAGATG GTGGAGGCTG CCAAGGGAGC AGCCGCCAAT
    CCTGACAGTG AGGAGCAACA GCAGCGGCTG CGGGAGGCAG CAGAGGGTCT CCGCATGGCA
    ACCAATGCCG CTGCACAGAA CGCCATCAAG AAAAAGCTGG TGCAGCGCCT TGAGCATGCA
    GCCAAACAGG CTGCAGCCTC GGCTACACAG ACTATCGCAG CAGCCCAACA TGCAACCTCC
    ACTCCCAAGG CCTCTGCTGG CCCCCAGCCC CTGCTGGTGC AGAGCTGCAA GGCTGTGGCA
    GAGCAGATTC CACTGCTGGT GCAGGGCGTC CGAGGGAGCC AAGCGCAGCC TGACAGCCCC
    AGTGCTCAGC TAGCCCTCAT CGCTGCCAGC CAAAGCTTCC TGCAGCCAGG TGGGAAGATG
    GTGGCAGCCG CAAAGGCCTC TGTGCCAACA ATTCAGGACC AAGCATCGGC CATGCAGCTG
    AGTCAGTGTG CTAAAAACCT TGGCACCGCG TTGGCTGAAC TCCGTACTGC TGCCCAGAAG
    GCTCAGGAAG CGTGTGGGCC TTTGGAAATG GATTCTGCTC TGAGTGTGGT GCAGAATCTG
    GAGAGAGACC TGCAGGAAGT GAAGGCAGCT GCTCGAGACG GCAAGCTGAA GCCCTTGCCT
    GGGGAGACGA TGGAGAAATG TGCCCAGGAC CTGGGCAACA GCACCAAAGC CGTGAGCTCC
    GCTATTGCCC AGCTGCTGGG GGAGGTCGCC CAGGGCAATG AGAATTACGC AGGCATTGCA
    GCTCGGGATG TGGCAGGTGG GCTGCGGTCA CTGGCCCAAG CTGCTAGGGG CGTAGCTGCC
    CTGACATCAG ATCCTGCAGT GCAAGCCATT GTGCTCGACA CAGCCAGCGA TGTCCTGGAC
    AAGGCCAGCA GCCTCATCGA GGAGGCAAAG AAAGCAGCCG GCCATCCAGG GGACCCTGAG
    AGCCAGCAGC GGCTTGCCCA GGTGGCTAAA GCTGTGACCC AGGCCCTGAA CCGTTGCGTC
    AGCTGCCTGC CTGGCCAGCG TGATGTGGAC AATGCCCTGA GGGCAGTGGG AGATGCCAGC
    AAGCGACTCC TGAGTGACTC GCTTCCCCCC AGCACTGGGA CCTTTCAGGA GGCTCAGAGC
    CGGCTGAATG AAGCCGCTGC CGGGCTGAAT CAGGCAGCCA CAGAGCTGGT GCAGGCCTCT
    CGGGGAACCC CTCAGGACCT GGCCCGAGCC TCAGGTCGAT TTGGACAAGA CTTCAGCACC
    TTCCTGGAAG CCGGCGTGGA GATGGCAGGC CAGGCTCCGA GCCAGGAGGA CCGTGCCCAG
    GTCGTATCCA ACTTGAAGGG CATCTCCATG TCTTCAAGCA AACTTCTTCT CGCTGCCAAG
    GCCCTGTCCA CAGACCCTGC AGCCCCAACC CTCAAGAGTC AGCTGGCTGC AGCTGCCCGG
    GCAGTGACCG ACAGCATCAA CCAGCTCATC AGCATGTGCA CCCAGCAGGC GCCAGGCCAG
    AAGGAGTGTG ATAATGCCCT GCGGGAATTG GAGACGGTCC GGGAACTCCT AGAGAACCCA
    GTCCAGCCCA TCAATGACAT GTCCTACTTT GGCTGTCTGG ACAGCGTCAT GGAAAACTCA
    AAGGTGCTGG GTGAGGCCAT GACCGGCATC TCCCAAAATG CCAAGAACGG AAACTTGCCG
    GAGTTTGGGG AGGCTGTCGC CACAGCCTCC AAGGCCCTCT GCGGCTTCAC CGAGGCAGCT
    GCACAGGCTG CATATCTGGT TGGCGTGTCT GACCCCAACA GCCAAGCTGG ACAACAAGGG
    CTGGTGGAGC CCACACAGTT TGCCCGAGCA AACCAGGCAA TTCAGATGGC CTGTCAGAGT
    TTGGGGGAGC CTGGCTGCAC CCAGGCCCAG GTGCTCTCTG CAGCCACCAT TGTGGCCAAA
    CACACGTCTG CGCTGTGTAA CAGCTGCCGC CTGGCTTCTG CCCGAACCGC CAATCCCACT
    GCCAAGCGCC AGTTCGTACA GTCAGCCAAG GAGGTGGCCA ACAGCACGGC CAACCTTGTC
    AAGACCATCA AGGCGCTGGA TGGGGCCTTC ACAGAGGAGA ACCGTGCCCA GTGCCGAGCA
    GCAACGGCCC CTTTGCTGGA GGCTGTGGAC AATCTGAGCG CCTTTGCGTC CAACCCTGAG
    TTCTCCAGTG TTCCTGCCCA GATCAGCCCT GAGGGCCGGG CTGCCATGGA GCCCATTGTG
    ATCTCTGCTA AGACAATGTT GGAGAGTGCT GGGGGCCTTA TTCAGACAGC CCGAGCCCTG
    GCGGTCAATC CGCGGGACCC ACCACGCTGG TCCGTGCTGG CTGGCCACTC CCGTACTGTC
    TCAGACTCCA TCAAGAAGCT GATCACAAGC ATGAGGGACA AGGCCCCAGG GCAGTTGGAG
    TGTGAGACAG CCATCGCAGC TCTGAACAGC TGTCTGCGGG ACCTTGACCA GGCTTCTCTT
    GCTGCAGTCA GCCAGCAGCT CGCTCCCCGT GAGGGGATCT CTCAGGAGGC CTTGCACACC
    CAGATGCTCA CTGCGGTGCA GGAAATCTCC CATCTTATTG AGCCGCTGGC CAGCGCTGCC
    CGGGCTGAAG CCTCCCAGCT GGGACACAAG GTGTCCCAGA TGGCCCAGTA CTTTGAGCCG
    CTCACTCTGG CTGCCGTGGG TGCTGCCTCC AAAACCCTGA GCCACCCACA GCAGATGGCG
    CTCCTGGACC AGACCAAAAC CTTGGCCGAG TCTGCCCTGC AGCTGCTATA CACAGCCAAG
    GAAGCTGGCG GTAACCCCAA GCAAGCAGCT CACACCCAGG AAGCCCTGGA GGAGGCTGTG
    CAGATGATGA CAGAGGCCGT AGAGGACCTG ACAACCACCC TCAACGAGGC AGCCAGTGCT
    GCCGGGGTCG TCGGTGGCAT GGTGGACTCC ATCACCCAGG CCATCAACCA GCTAGATGAA
    GGACCAATGG GTGAACCAGA GGGTTCCTTC GTGGATTACC AGACCACCAT GGTGCGGACA
    GCCAAGGCCA TTGCTGTCAC CGTTCAGGAG ATGGTAACCA AGTCAAACAC CAGCCCTGAG
    GAGCTGGGCC CTCTCGCTAA CCAGCTGACT AGTGACTATG GGCGTCTTGC CTCCCAGGCC
    AAGCCTGCAG CTGTGGCTGC TGAAAATGAA GAGATAGGCG CCCACATCAA GCACCGGGTT
    CAGGAGCTGG GGCACGGCTG CGCAGCTCTG GTCACCAAGG CAGGCGCCCT GCAGTGTAGC
    CCTAGTGATG CCTACACCAA GAAGGAGCTC ATAGAGTGTG CCCGGAGAGT CTCTGAGAAG
    GTCTCCCACG TGCTGGCTGC GCTCCAGGCT GGGAATCGTG GCACCCAGGC CTGCATCACA
    GCCGCCAGCG CTGTGTCCGG CATCATCGCC GACCTGGACA CCACCATCAT GTTCGCCACT
    GCCGGCACAC TCAACCGTGA GGGTGCCGAA ACCTTTGCTG ACCACCGAGA GGGCATTCTG
    AAGACTGCCA AGGTGCTGGT GGAAGACACC AAGGTCCTGG TGCAGAATGC AGCTGGGAGC
    CAGGAGAAGC TGGCACAGGC AGCCCAGTCC TCCGTGGCCA CCATCACCCG CCTCGCCGAT
    GTGGTCAAGC TGGGTGCAGC CAGCCTGGGA GCTGAGGACC CAGAGACCCA GGTGGTGCTG
    ATCAATGCAG TGAAAGATGT GGCCAAGGCC CTGGGAGACC TCATCAGTGC CACAAAGGCT
    GCGGCTGGCA AAGTCGGCGA CGACCCTGCT GTGTGGCAGC TCAAGAACTC TGCCAAGGTG
    ATGGTAACCA ATGTGACATC ACTGCTCAAG ACCGTGAAAG CGGTAGAGGA TGAGGCCACC
    AAAGGCACGC GCGCCCTGGA GGCCACAACG GAGCATATTC GGCAGGAGCT GGCGGTCTTC
    TGCTCCCCAG AGCCACCTGC CAAGACCTCG ACCCCTGAAG ATTTCATCCG CATGACCAAG
    GGTATCACCA TGGCAACCGC CAAGGCCGTT GCCGCTGGGA ACTCCTGTCG CCAGGAGGAT
    GTCATTGCCA CAGCCAATCT GAGTCGCCGT GCTATTGCAG ATATGCTCCG GGCTTGCAAG
    GAAGCTGCCT ACCACCCAGA AGTGGCTCCA GACGTGCGGC TCCGAGCCCT ACACTTTGGC
    CGGGAATGTG CCAATGGCTA CCTGGAACTG CTGGACCACG TGCTGCTGAC CCTGCAGAAG
    CCAAGCCCAG AATTGAAGCA GCAATTGACG GTGCACTCAA AGCGTGTGGC TGGATCAGTC
    ACTGAGCTCA TCCAGGCTGC TGAGGCCATG AAGGGAACAG AATGGGTGGA CCCAGAGGAC
    CCCACAGTCA TTGCTGAGAA TGAACTCCTG GGAGCCGCGG CTGCCATTGA GGCTGCGGCC
    AAGAAGCTGG AGCAGCTGAA GCCCCGGGCC AAACCTAAGG AGGCGGACGA GTCCTTGAAC
    TTTGAGGAGC AGATACTGGA AGCTGCCAAG TCCATCGCAG CAGCCACCAG CGCCCTGGTG
    AAGGCTGCCT CAGCTGCACA GAGGGAACTG GTGGCCCAGG GGAAGGTGGG CGCCATTCCA
    GCCAACGCAC TGGATGATGG ACAGTGGTCC CAGGGCCTCA TCTCTGCTGC CCGGATGGTG
    GCTGCGGCCA CCAACAACCT GTGTGAGGCA GCCAATGCAG CTGTCCAAGG CCACGCCAGC
    CAGGAGAAGC TCATCTCATC AGCCAAGCAG GTCGCCGCCT CCACAGCCCA GCTCCTCGTA
    GCCTGCAAGG TCAAGGCCGA CCAGGACTCT GAGGCAATGA AGCGGCTTCA GGCTGCCGGC
    AACGCAGTGA AGCGAGCCTC AGACAACCTG GTGAAGGCAG CCCAGAAGGC TGCAGCCTTT
    GAAGAACAGG AGAATGAGAC CGTGGTGGTG AAGGAGAAGA TGGTCGGGGG CATTGCCCAG
    ATCATTGCTG CCCAGGAAGA GATGCTTCGG AAGGAACGAG AGCTGGAGGA GGCACGGAAG
    AAGCTGGCGC AGATCCGGCA GCAGCAGTAC AAATTCTTAC CTTCAGAGCT TCGAGACGAG
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237296
    Clone ID Related Accession (Same CDS sequence) NM_001205428.3 , XM_024995995.1
    Accession Version NM_001205428.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product talin-1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000008.1. On May 30, 2018 this sequence version replaced NM_001205428.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.49053.1, SRR6764281.39687.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTTGCAC TTTCGCTGAA GATCAGCATT GGGAATGTGG TGAAGACGAT GCAATTTGAG 
    CCGTCTACCA TGGTGTATGA CGCCTGTCGG ATCATCCGTG AGCGGATCCC AGAGGCCCTA
    TCTGGCCCTC CCAGCGACTT TGGGCTGTTT CTGTCAGATG ATGACCCCAA AAAGGGTATA
    TGGCTGGAGG CAGGGAAGGC TTTGGATTAT TACATGCTCC GAAACGGGGA CACCATGGAG
    TATAGGAAGA AACAGAGGCC CTTGAAGATC CGCATGCTGG ACGGCACCGT GAAGACTGTC
    ATGGTGGACG ACTCGAAGAC TGTCACGGAC ATGCTCATGA CCATCTGTGC CCGCATTGGC
    ATCACCAACC ATGATGAATA TTCACTGGTT CGAGAGATTA TGGAAGAGAA GAAGGAGGAG
    GTGACAGGGA CCTTACGAAA GGACAAGACA CTGCTGCGAG ACGAAAAGAA GATGGAGAAA
    CTGAAGCAGA AGTTGCACAC AGATGACGAG TTGAACTGGC TGGACCATGG CCGGACCCTG
    AGGGAGCAGG GAGTGGAGGA GCACGAAACG CTTCTGCTCC GGAGGAAGTT CTTCTACTCA
    GATCAGAACG TGGACTCCCG GGACCCCGTC CAGCTGAACC TTCTCTACGT GCAGGCACGA
    GATGACATCC TGAATGGCTC CCATCCTGTC TCCTTCGACA AGGCCTGTGA GTTTGCTGGC
    TTCCAATGCC AGATCCAGTT TGGGCCCCAC AATGAGCAGA AGCACAAGGC CGGCTTCCTC
    GACCTGAAGG ACTTCCTGCC CAAGGAGTAT GTGAAGCAGA AGGGAGAGCG TAAGATCTTC
    CAGGCACACA AGAACTGTGG ACAGATGAGT GAGATTGAGG CCAAGGTACG CTACGTGAAG
    CTAGCCCGTT CCCTCAAGAC GTACGGTGTC TCCTTCTTCC TGGTCAAGGA AAAGATGAAA
    GGAAAGAACA AGTTGGTGCC CAGGCTTCTG GGCATCACTA AGGAGTGTGT GATGCGAGTG
    GACGAGAAGA CCAAGGAGGT GATCCAGGAA TGGAACCTCA CCAACATAAA ACGCTGGGCT
    GCGTCTCCCA AGAGCTTCAC CCTGGATTTC GGAGACTACC AAGATGGCTA CTACTCGGTG
    CAGACGACCG AGGGGGAGCA GATCGCTCAG CTCATCGCTG GCTACATCGA CATCATCCTT
    AAGAAGAAAA AAAGCAAGGA TCATTTTGGG CTGGAGGGAG ACGAGGAGTC TACTATGCTG
    GAGGACTCCG TGTCCCCCAA AAAGTCCACT GTGCTTCAGC AGCAGTATAA CCGGGTGGGG
    AAGGTGGAGC ATGGCTCCGT GGCCCTGCCC GCCATCATGC GCTCCGGAGC CTCGGGTCCC
    GAGAATTTCC AGGTGGGCAG CATGCCACCT GCCCAGCAGC AGATCACCAG TGGCCAGATG
    CACCGAGGAC ACATGCCTCC TTTGACGTCA GCCCAGCAGG CGCTCACTGG GACCATCAAC
    TCCAGCATGC AGGCTGTGCA AGCTGCCCAG GCCACACTGG ATGACTTTGA CACTCTGCCC
    CCTCTGGGCC AGGACGCTGC CTCAAAGGCC TGGCGTAAGA ACAAGATGGA TGAATCCAAG
    CATGAAATCC ACTCCCAGGT AGACGCCATC ACCGCTGGTA CCGCCTCTGT GGTGAACCTC
    ACAGCAGGGG ACCCTGCTGA GACAGACTAC ACGGCAGTGG GCTGTGCGGT CACCACCATC
    TCCTCCAACC TGACGGAGAT GTCCCGTGGC GTGAAGCTGC TTGCTGCCCT GCTGGAGGAT
    GAAGGCGGCA GCGGCCGGCC CCTGCTGCAG GCGGCCAAGG GCCTGGCGGG GGCGGTGTCA
    GAGCTGCTGC GCAGTGCCCA GCCAGCCAGT GCCGAGCCCC GTCAGAACCT GCTGCAAGCA
    GCTGGGAACG TGGGCCAGGC CAGTGGGGAG CTGTTGCAAC AAATTGGGGA AAGTGATACT
    GACCCCCACT TCCAGGACGT GCTCATGCAG CTAGCCAAGG CCGTGGCAAG CGCTGCAGCT
    GCCCTGGTCC TCAAGGCCAA GAGTGTGGCC CAGCGAACAG AGGACTCAGG GCTTCAGACC
    CAGGTCATCG CTGCGGCAAC ACAGTGTGCC CTGTCCACCT CCCAGCTGGT GGCCTGTACC
    AAGGTGGTGG CTCCTACTAT CAGCTCCCCA GTCTGCCAAG AACAGCTGGT AGAGGCTGGG
    CGACTAGTGG CCAAGGCTGT GGAGGGCTGT GTGTCCGCTT CCCAGGCCGC CACAGAGGAT
    GCCCAGCTGT TACGGGGGGT AGGTGCTGCG GCCACAGCTG TCACCCAGGC CCTGAATGAG
    CTGCTGCAGC ACGTGAGGGC CCATGCCACA GGGGCTGGGC CTGCTGGCCG CTACGACCAG
    GCCACTGACA CCATCCTCAC TGTCACCGAG AACATCTTCA GCTCCATGGG TGATGCTGGT
    GAGATGGTGC GACAGGCCCG CATCCTCGCC CAAGCCACCT CTGACCTGGT GAACGCCATC
    AAGGCTGATG CCGAAGGGGA GAGTGATCTG GAGAATTCCC GCAAGCTGTT AAGTGCCGCC
    AAGATCCTGG CCGATGCCAC AGCCAAGATG GTGGAGGCTG CCAAGGGAGC AGCCGCCAAT
    CCTGACAGTG AGGAGCAACA GCAGCGGCTG CGGGAGGCAG CAGAGGGTCT CCGCATGGCA
    ACCAATGCCG CTGCACAGAA CGCCATCAAG AAAAAGCTGG TGCAGCGCCT TGAGCATGCA
    GCCAAACAGG CTGCAGCCTC GGCTACACAG ACTATCGCAG CAGCCCAACA TGCAACCTCC
    ACTCCCAAGG CCTCTGCTGG CCCCCAGCCC CTGCTGGTGC AGAGCTGCAA GGCTGTGGCA
    GAGCAGATTC CACTGCTGGT GCAGGGCGTC CGAGGGAGCC AAGCGCAGCC TGACAGCCCC
    AGTGCTCAGC TAGCCCTCAT CGCTGCCAGC CAAAGCTTCC TGCAGCCAGG TGGGAAGATG
    GTGGCAGCCG CAAAGGCCTC TGTGCCAACA ATTCAGGACC AAGCATCGGC CATGCAGCTG
    AGTCAGTGTG CTAAAAACCT TGGCACCGCG TTGGCTGAAC TCCGTACTGC TGCCCAGAAG
    GCTCAGGAAG CGTGTGGGCC TTTGGAAATG GATTCTGCTC TGAGTGTGGT GCAGAATCTG
    GAGAGAGACC TGCAGGAAGT GAAGGCAGCT GCTCGAGACG GCAAGCTGAA GCCCTTGCCT
    GGGGAGACGA TGGAGAAATG TGCCCAGGAC CTGGGCAACA GCACCAAAGC CGTGAGCTCC
    GCTATTGCCC AGCTGCTGGG GGAGGTCGCC CAGGGCAATG AGAATTACGC AGGCATTGCA
    GCTCGGGATG TGGCAGGTGG GCTGCGGTCA CTGGCCCAAG CTGCTAGGGG CGTAGCTGCC
    CTGACATCAG ATCCTGCAGT GCAAGCCATT GTGCTCGACA CAGCCAGCGA TGTCCTGGAC
    AAGGCCAGCA GCCTCATCGA GGAGGCAAAG AAAGCAGCCG GCCATCCAGG GGACCCTGAG
    AGCCAGCAGC GGCTTGCCCA GGTGGCTAAA GCTGTGACCC AGGCCCTGAA CCGTTGCGTC
    AGCTGCCTGC CTGGCCAGCG TGATGTGGAC AATGCCCTGA GGGCAGTGGG AGATGCCAGC
    AAGCGACTCC TGAGTGACTC GCTTCCCCCC AGCACTGGGA CCTTTCAGGA GGCTCAGAGC
    CGGCTGAATG AAGCCGCTGC CGGGCTGAAT CAGGCAGCCA CAGAGCTGGT GCAGGCCTCT
    CGGGGAACCC CTCAGGACCT GGCCCGAGCC TCAGGTCGAT TTGGACAAGA CTTCAGCACC
    TTCCTGGAAG CCGGCGTGGA GATGGCAGGC CAGGCTCCGA GCCAGGAGGA CCGTGCCCAG
    GTCGTATCCA ACTTGAAGGG CATCTCCATG TCTTCAAGCA AACTTCTTCT CGCTGCCAAG
    GCCCTGTCCA CAGACCCTGC AGCCCCAACC CTCAAGAGTC AGCTGGCTGC AGCTGCCCGG
    GCAGTGACCG ACAGCATCAA CCAGCTCATC AGCATGTGCA CCCAGCAGGC GCCAGGCCAG
    AAGGAGTGTG ATAATGCCCT GCGGGAATTG GAGACGGTCC GGGAACTCCT AGAGAACCCA
    GTCCAGCCCA TCAATGACAT GTCCTACTTT GGCTGTCTGG ACAGCGTCAT GGAAAACTCA
    AAGGTGCTGG GTGAGGCCAT GACCGGCATC TCCCAAAATG CCAAGAACGG AAACTTGCCG
    GAGTTTGGGG AGGCTGTCGC CACAGCCTCC AAGGCCCTCT GCGGCTTCAC CGAGGCAGCT
    GCACAGGCTG CATATCTGGT TGGCGTGTCT GACCCCAACA GCCAAGCTGG ACAACAAGGG
    CTGGTGGAGC CCACACAGTT TGCCCGAGCA AACCAGGCAA TTCAGATGGC CTGTCAGAGT
    TTGGGGGAGC CTGGCTGCAC CCAGGCCCAG GTGCTCTCTG CAGCCACCAT TGTGGCCAAA
    CACACGTCTG CGCTGTGTAA CAGCTGCCGC CTGGCTTCTG CCCGAACCGC CAATCCCACT
    GCCAAGCGCC AGTTCGTACA GTCAGCCAAG GAGGTGGCCA ACAGCACGGC CAACCTTGTC
    AAGACCATCA AGGCGCTGGA TGGGGCCTTC ACAGAGGAGA ACCGTGCCCA GTGCCGAGCA
    GCAACGGCCC CTTTGCTGGA GGCTGTGGAC AATCTGAGCG CCTTTGCGTC CAACCCTGAG
    TTCTCCAGTG TTCCTGCCCA GATCAGCCCT GAGGGCCGGG CTGCCATGGA GCCCATTGTG
    ATCTCTGCTA AGACAATGTT GGAGAGTGCT GGGGGCCTTA TTCAGACAGC CCGAGCCCTG
    GCGGTCAATC CGCGGGACCC ACCACGCTGG TCCGTGCTGG CTGGCCACTC CCGTACTGTC
    TCAGACTCCA TCAAGAAGCT GATCACAAGC ATGAGGGACA AGGCCCCAGG GCAGTTGGAG
    TGTGAGACAG CCATCGCAGC TCTGAACAGC TGTCTGCGGG ACCTTGACCA GGCTTCTCTT
    GCTGCAGTCA GCCAGCAGCT CGCTCCCCGT GAGGGGATCT CTCAGGAGGC CTTGCACACC
    CAGATGCTCA CTGCGGTGCA GGAAATCTCC CATCTTATTG AGCCGCTGGC CAGCGCTGCC
    CGGGCTGAAG CCTCCCAGCT GGGACACAAG GTGTCCCAGA TGGCCCAGTA CTTTGAGCCG
    CTCACTCTGG CTGCCGTGGG TGCTGCCTCC AAAACCCTGA GCCACCCACA GCAGATGGCG
    CTCCTGGACC AGACCAAAAC CTTGGCCGAG TCTGCCCTGC AGCTGCTATA CACAGCCAAG
    GAAGCTGGCG GTAACCCCAA GCAAGCAGCT CACACCCAGG AAGCCCTGGA GGAGGCTGTG
    CAGATGATGA CAGAGGCCGT AGAGGACCTG ACAACCACCC TCAACGAGGC AGCCAGTGCT
    GCCGGGGTCG TCGGTGGCAT GGTGGACTCC ATCACCCAGG CCATCAACCA GCTAGATGAA
    GGACCAATGG GTGAACCAGA GGGTTCCTTC GTGGATTACC AGACCACCAT GGTGCGGACA
    GCCAAGGCCA TTGCTGTCAC CGTTCAGGAG ATGGTAACCA AGTCAAACAC CAGCCCTGAG
    GAGCTGGGCC CTCTCGCTAA CCAGCTGACT AGTGACTATG GGCGTCTTGC CTCCCAGGCC
    AAGCCTGCAG CTGTGGCTGC TGAAAATGAA GAGATAGGCG CCCACATCAA GCACCGGGTT
    CAGGAGCTGG GGCACGGCTG CGCAGCTCTG GTCACCAAGG CAGGCGCCCT GCAGTGTAGC
    CCTAGTGATG CCTACACCAA GAAGGAGCTC ATAGAGTGTG CCCGGAGAGT CTCTGAGAAG
    GTCTCCCACG TGCTGGCTGC GCTCCAGGCT GGGAATCGTG GCACCCAGGC CTGCATCACA
    GCCGCCAGCG CTGTGTCCGG CATCATCGCC GACCTGGACA CCACCATCAT GTTCGCCACT
    GCCGGCACAC TCAACCGTGA GGGTGCCGAA ACCTTTGCTG ACCACCGAGA GGGCATTCTG
    AAGACTGCCA AGGTGCTGGT GGAAGACACC AAGGTCCTGG TGCAGAATGC AGCTGGGAGC
    CAGGAGAAGC TGGCACAGGC AGCCCAGTCC TCCGTGGCCA CCATCACCCG CCTCGCCGAT
    GTGGTCAAGC TGGGTGCAGC CAGCCTGGGA GCTGAGGACC CAGAGACCCA GGTGGTGCTG
    ATCAATGCAG TGAAAGATGT GGCCAAGGCC CTGGGAGACC TCATCAGTGC CACAAAGGCT
    GCGGCTGGCA AAGTCGGCGA CGACCCTGCT GTGTGGCAGC TCAAGAACTC TGCCAAGGTG
    ATGGTAACCA ATGTGACATC ACTGCTCAAG ACCGTGAAAG CGGTAGAGGA TGAGGCCACC
    AAAGGCACGC GCGCCCTGGA GGCCACAACG GAGCATATTC GGCAGGAGCT GGCGGTCTTC
    TGCTCCCCAG AGCCACCTGC CAAGACCTCG ACCCCTGAAG ATTTCATCCG CATGACCAAG
    GGTATCACCA TGGCAACCGC CAAGGCCGTT GCCGCTGGGA ACTCCTGTCG CCAGGAGGAT
    GTCATTGCCA CAGCCAATCT GAGTCGCCGT GCTATTGCAG ATATGCTCCG GGCTTGCAAG
    GAAGCTGCCT ACCACCCAGA AGTGGCTCCA GACGTGCGGC TCCGAGCCCT ACACTTTGGC
    CGGGAATGTG CCAATGGCTA CCTGGAACTG CTGGACCACG TGCTGCTGAC CCTGCAGAAG
    CCAAGCCCAG AATTGAAGCA GCAATTGACG GTGCACTCAA AGCGTGTGGC TGGATCAGTC
    ACTGAGCTCA TCCAGGCTGC TGAGGCCATG AAGGGAACAG AATGGGTGGA CCCAGAGGAC
    CCCACAGTCA TTGCTGAGAA TGAACTCCTG GGAGCCGCGG CTGCCATTGA GGCTGCGGCC
    AAGAAGCTGG AGCAGCTGAA GCCCCGGGCC AAACCTAAGG AGGCGGACGA GTCCTTGAAC
    TTTGAGGAGC AGATACTGGA AGCTGCCAAG TCCATCGCAG CAGCCACCAG CGCCCTGGTG
    AAGGCTGCCT CAGCTGCACA GAGGGAACTG GTGGCCCAGG GGAAGGTGGG CGCCATTCCA
    GCCAACGCAC TGGATGATGG ACAGTGGTCC CAGGGCCTCA TCTCTGCTGC CCGGATGGTG
    GCTGCGGCCA CCAACAACCT GTGTGAGGCA GCCAATGCAG CTGTCCAAGG CCACGCCAGC
    CAGGAGAAGC TCATCTCATC AGCCAAGCAG GTCGCCGCCT CCACAGCCCA GCTCCTCGTA
    GCCTGCAAGG TCAAGGCCGA CCAGGACTCT GAGGCAATGA AGCGGCTTCA GGCTGCCGGC
    AACGCAGTGA AGCGAGCCTC AGACAACCTG GTGAAGGCAG CCCAGAAGGC TGCAGCCTTT
    GAAGAACAGG AGAATGAGAC CGTGGTGGTG AAGGAGAAGA TGGTCGGGGG CATTGCCCAG
    ATCATTGCTG CCCAGGAAGA GATGCTTCGG AAGGAACGAG AGCTGGAGGA GGCACGGAAG
    AAGCTGGCGC AGATCCGGCA GCAGCAGTAC AAATTCTTAC CTTCAGAGCT TCGAGACGAG
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192357.3
    CDS319..7944
    Translation

    Target ORF information:

    RefSeq Version NM_001205428.3
    Organism Bos taurus(cattle)
    Definition Bos taurus talin 1 (TLN1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205428.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTTGCAC TTTCGCTGAA GATCAGCATT GGGAATGTGG TGAAGACGAT GCAATTTGAG 
    CCGTCTACCA TGGTGTATGA CGCCTGTCGG ATCATCCGTG AGCGGATCCC AGAGGCCCTA
    TCTGGCCCTC CCAGCGACTT TGGGCTGTTT CTGTCAGATG ATGACCCCAA AAAGGGTATA
    TGGCTGGAGG CAGGGAAGGC TTTGGATTAT TACATGCTCC GAAACGGGGA CACCATGGAG
    TATAGGAAGA AACAGAGGCC CTTGAAGATC CGCATGCTGG ACGGCACCGT GAAGACTGTC
    ATGGTGGACG ACTCGAAGAC TGTCACGGAC ATGCTCATGA CCATCTGTGC CCGCATTGGC
    ATCACCAACC ATGATGAATA TTCACTGGTT CGAGAGATTA TGGAAGAGAA GAAGGAGGAG
    GTGACAGGGA CCTTACGAAA GGACAAGACA CTGCTGCGAG ACGAAAAGAA GATGGAGAAA
    CTGAAGCAGA AGTTGCACAC AGATGACGAG TTGAACTGGC TGGACCATGG CCGGACCCTG
    AGGGAGCAGG GAGTGGAGGA GCACGAAACG CTTCTGCTCC GGAGGAAGTT CTTCTACTCA
    GATCAGAACG TGGACTCCCG GGACCCCGTC CAGCTGAACC TTCTCTACGT GCAGGCACGA
    GATGACATCC TGAATGGCTC CCATCCTGTC TCCTTCGACA AGGCCTGTGA GTTTGCTGGC
    TTCCAATGCC AGATCCAGTT TGGGCCCCAC AATGAGCAGA AGCACAAGGC CGGCTTCCTC
    GACCTGAAGG ACTTCCTGCC CAAGGAGTAT GTGAAGCAGA AGGGAGAGCG TAAGATCTTC
    CAGGCACACA AGAACTGTGG ACAGATGAGT GAGATTGAGG CCAAGGTACG CTACGTGAAG
    CTAGCCCGTT CCCTCAAGAC GTACGGTGTC TCCTTCTTCC TGGTCAAGGA AAAGATGAAA
    GGAAAGAACA AGTTGGTGCC CAGGCTTCTG GGCATCACTA AGGAGTGTGT GATGCGAGTG
    GACGAGAAGA CCAAGGAGGT GATCCAGGAA TGGAACCTCA CCAACATAAA ACGCTGGGCT
    GCGTCTCCCA AGAGCTTCAC CCTGGATTTC GGAGACTACC AAGATGGCTA CTACTCGGTG
    CAGACGACCG AGGGGGAGCA GATCGCTCAG CTCATCGCTG GCTACATCGA CATCATCCTT
    AAGAAGAAAA AAAGCAAGGA TCATTTTGGG CTGGAGGGAG ACGAGGAGTC TACTATGCTG
    GAGGACTCCG TGTCCCCCAA AAAGTCCACT GTGCTTCAGC AGCAGTATAA CCGGGTGGGG
    AAGGTGGAGC ATGGCTCCGT GGCCCTGCCC GCCATCATGC GCTCCGGAGC CTCGGGTCCC
    GAGAATTTCC AGGTGGGCAG CATGCCACCT GCCCAGCAGC AGATCACCAG TGGCCAGATG
    CACCGAGGAC ACATGCCTCC TTTGACGTCA GCCCAGCAGG CGCTCACTGG GACCATCAAC
    TCCAGCATGC AGGCTGTGCA AGCTGCCCAG GCCACACTGG ATGACTTTGA CACTCTGCCC
    CCTCTGGGCC AGGACGCTGC CTCAAAGGCC TGGCGTAAGA ACAAGATGGA TGAATCCAAG
    CATGAAATCC ACTCCCAGGT AGACGCCATC ACCGCTGGTA CCGCCTCTGT GGTGAACCTC
    ACAGCAGGGG ACCCTGCTGA GACAGACTAC ACGGCAGTGG GCTGTGCGGT CACCACCATC
    TCCTCCAACC TGACGGAGAT GTCCCGTGGC GTGAAGCTGC TTGCTGCCCT GCTGGAGGAT
    GAAGGCGGCA GCGGCCGGCC CCTGCTGCAG GCGGCCAAGG GCCTGGCGGG GGCGGTGTCA
    GAGCTGCTGC GCAGTGCCCA GCCAGCCAGT GCCGAGCCCC GTCAGAACCT GCTGCAAGCA
    GCTGGGAACG TGGGCCAGGC CAGTGGGGAG CTGTTGCAAC AAATTGGGGA AAGTGATACT
    GACCCCCACT TCCAGGACGT GCTCATGCAG CTAGCCAAGG CCGTGGCAAG CGCTGCAGCT
    GCCCTGGTCC TCAAGGCCAA GAGTGTGGCC CAGCGAACAG AGGACTCAGG GCTTCAGACC
    CAGGTCATCG CTGCGGCAAC ACAGTGTGCC CTGTCCACCT CCCAGCTGGT GGCCTGTACC
    AAGGTGGTGG CTCCTACTAT CAGCTCCCCA GTCTGCCAAG AACAGCTGGT AGAGGCTGGG
    CGACTAGTGG CCAAGGCTGT GGAGGGCTGT GTGTCCGCTT CCCAGGCCGC CACAGAGGAT
    GCCCAGCTGT TACGGGGGGT AGGTGCTGCG GCCACAGCTG TCACCCAGGC CCTGAATGAG
    CTGCTGCAGC ACGTGAGGGC CCATGCCACA GGGGCTGGGC CTGCTGGCCG CTACGACCAG
    GCCACTGACA CCATCCTCAC TGTCACCGAG AACATCTTCA GCTCCATGGG TGATGCTGGT
    GAGATGGTGC GACAGGCCCG CATCCTCGCC CAAGCCACCT CTGACCTGGT GAACGCCATC
    AAGGCTGATG CCGAAGGGGA GAGTGATCTG GAGAATTCCC GCAAGCTGTT AAGTGCCGCC
    AAGATCCTGG CCGATGCCAC AGCCAAGATG GTGGAGGCTG CCAAGGGAGC AGCCGCCAAT
    CCTGACAGTG AGGAGCAACA GCAGCGGCTG CGGGAGGCAG CAGAGGGTCT CCGCATGGCA
    ACCAATGCCG CTGCACAGAA CGCCATCAAG AAAAAGCTGG TGCAGCGCCT TGAGCATGCA
    GCCAAACAGG CTGCAGCCTC GGCTACACAG ACTATCGCAG CAGCCCAACA TGCAACCTCC
    ACTCCCAAGG CCTCTGCTGG CCCCCAGCCC CTGCTGGTGC AGAGCTGCAA GGCTGTGGCA
    GAGCAGATTC CACTGCTGGT GCAGGGCGTC CGAGGGAGCC AAGCGCAGCC TGACAGCCCC
    AGTGCTCAGC TAGCCCTCAT CGCTGCCAGC CAAAGCTTCC TGCAGCCAGG TGGGAAGATG
    GTGGCAGCCG CAAAGGCCTC TGTGCCAACA ATTCAGGACC AAGCATCGGC CATGCAGCTG
    AGTCAGTGTG CTAAAAACCT TGGCACCGCG TTGGCTGAAC TCCGTACTGC TGCCCAGAAG
    GCTCAGGAAG CGTGTGGGCC TTTGGAAATG GATTCTGCTC TGAGTGTGGT GCAGAATCTG
    GAGAGAGACC TGCAGGAAGT GAAGGCAGCT GCTCGAGACG GCAAGCTGAA GCCCTTGCCT
    GGGGAGACGA TGGAGAAATG TGCCCAGGAC CTGGGCAACA GCACCAAAGC CGTGAGCTCC
    GCTATTGCCC AGCTGCTGGG GGAGGTCGCC CAGGGCAATG AGAATTACGC AGGCATTGCA
    GCTCGGGATG TGGCAGGTGG GCTGCGGTCA CTGGCCCAAG CTGCTAGGGG CGTAGCTGCC
    CTGACATCAG ATCCTGCAGT GCAAGCCATT GTGCTCGACA CAGCCAGCGA TGTCCTGGAC
    AAGGCCAGCA GCCTCATCGA GGAGGCAAAG AAAGCAGCCG GCCATCCAGG GGACCCTGAG
    AGCCAGCAGC GGCTTGCCCA GGTGGCTAAA GCTGTGACCC AGGCCCTGAA CCGTTGCGTC
    AGCTGCCTGC CTGGCCAGCG TGATGTGGAC AATGCCCTGA GGGCAGTGGG AGATGCCAGC
    AAGCGACTCC TGAGTGACTC GCTTCCCCCC AGCACTGGGA CCTTTCAGGA GGCTCAGAGC
    CGGCTGAATG AAGCCGCTGC CGGGCTGAAT CAGGCAGCCA CAGAGCTGGT GCAGGCCTCT
    CGGGGAACCC CTCAGGACCT GGCCCGAGCC TCAGGTCGAT TTGGACAAGA CTTCAGCACC
    TTCCTGGAAG CCGGCGTGGA GATGGCAGGC CAGGCTCCGA GCCAGGAGGA CCGTGCCCAG
    GTCGTATCCA ACTTGAAGGG CATCTCCATG TCTTCAAGCA AACTTCTTCT CGCTGCCAAG
    GCCCTGTCCA CAGACCCTGC AGCCCCAACC CTCAAGAGTC AGCTGGCTGC AGCTGCCCGG
    GCAGTGACCG ACAGCATCAA CCAGCTCATC AGCATGTGCA CCCAGCAGGC GCCAGGCCAG
    AAGGAGTGTG ATAATGCCCT GCGGGAATTG GAGACGGTCC GGGAACTCCT AGAGAACCCA
    GTCCAGCCCA TCAATGACAT GTCCTACTTT GGCTGTCTGG ACAGCGTCAT GGAAAACTCA
    AAGGTGCTGG GTGAGGCCAT GACCGGCATC TCCCAAAATG CCAAGAACGG AAACTTGCCG
    GAGTTTGGGG AGGCTGTCGC CACAGCCTCC AAGGCCCTCT GCGGCTTCAC CGAGGCAGCT
    GCACAGGCTG CATATCTGGT TGGCGTGTCT GACCCCAACA GCCAAGCTGG ACAACAAGGG
    CTGGTGGAGC CCACACAGTT TGCCCGAGCA AACCAGGCAA TTCAGATGGC CTGTCAGAGT
    TTGGGGGAGC CTGGCTGCAC CCAGGCCCAG GTGCTCTCTG CAGCCACCAT TGTGGCCAAA
    CACACGTCTG CGCTGTGTAA CAGCTGCCGC CTGGCTTCTG CCCGAACCGC CAATCCCACT
    GCCAAGCGCC AGTTCGTACA GTCAGCCAAG GAGGTGGCCA ACAGCACGGC CAACCTTGTC
    AAGACCATCA AGGCGCTGGA TGGGGCCTTC ACAGAGGAGA ACCGTGCCCA GTGCCGAGCA
    GCAACGGCCC CTTTGCTGGA GGCTGTGGAC AATCTGAGCG CCTTTGCGTC CAACCCTGAG
    TTCTCCAGTG TTCCTGCCCA GATCAGCCCT GAGGGCCGGG CTGCCATGGA GCCCATTGTG
    ATCTCTGCTA AGACAATGTT GGAGAGTGCT GGGGGCCTTA TTCAGACAGC CCGAGCCCTG
    GCGGTCAATC CGCGGGACCC ACCACGCTGG TCCGTGCTGG CTGGCCACTC CCGTACTGTC
    TCAGACTCCA TCAAGAAGCT GATCACAAGC ATGAGGGACA AGGCCCCAGG GCAGTTGGAG
    TGTGAGACAG CCATCGCAGC TCTGAACAGC TGTCTGCGGG ACCTTGACCA GGCTTCTCTT
    GCTGCAGTCA GCCAGCAGCT CGCTCCCCGT GAGGGGATCT CTCAGGAGGC CTTGCACACC
    CAGATGCTCA CTGCGGTGCA GGAAATCTCC CATCTTATTG AGCCGCTGGC CAGCGCTGCC
    CGGGCTGAAG CCTCCCAGCT GGGACACAAG GTGTCCCAGA TGGCCCAGTA CTTTGAGCCG
    CTCACTCTGG CTGCCGTGGG TGCTGCCTCC AAAACCCTGA GCCACCCACA GCAGATGGCG
    CTCCTGGACC AGACCAAAAC CTTGGCCGAG TCTGCCCTGC AGCTGCTATA CACAGCCAAG
    GAAGCTGGCG GTAACCCCAA GCAAGCAGCT CACACCCAGG AAGCCCTGGA GGAGGCTGTG
    CAGATGATGA CAGAGGCCGT AGAGGACCTG ACAACCACCC TCAACGAGGC AGCCAGTGCT
    GCCGGGGTCG TCGGTGGCAT GGTGGACTCC ATCACCCAGG CCATCAACCA GCTAGATGAA
    GGACCAATGG GTGAACCAGA GGGTTCCTTC GTGGATTACC AGACCACCAT GGTGCGGACA
    GCCAAGGCCA TTGCTGTCAC CGTTCAGGAG ATGGTAACCA AGTCAAACAC CAGCCCTGAG
    GAGCTGGGCC CTCTCGCTAA CCAGCTGACT AGTGACTATG GGCGTCTTGC CTCCCAGGCC
    AAGCCTGCAG CTGTGGCTGC TGAAAATGAA GAGATAGGCG CCCACATCAA GCACCGGGTT
    CAGGAGCTGG GGCACGGCTG CGCAGCTCTG GTCACCAAGG CAGGCGCCCT GCAGTGTAGC
    CCTAGTGATG CCTACACCAA GAAGGAGCTC ATAGAGTGTG CCCGGAGAGT CTCTGAGAAG
    GTCTCCCACG TGCTGGCTGC GCTCCAGGCT GGGAATCGTG GCACCCAGGC CTGCATCACA
    GCCGCCAGCG CTGTGTCCGG CATCATCGCC GACCTGGACA CCACCATCAT GTTCGCCACT
    GCCGGCACAC TCAACCGTGA GGGTGCCGAA ACCTTTGCTG ACCACCGAGA GGGCATTCTG
    AAGACTGCCA AGGTGCTGGT GGAAGACACC AAGGTCCTGG TGCAGAATGC AGCTGGGAGC
    CAGGAGAAGC TGGCACAGGC AGCCCAGTCC TCCGTGGCCA CCATCACCCG CCTCGCCGAT
    GTGGTCAAGC TGGGTGCAGC CAGCCTGGGA GCTGAGGACC CAGAGACCCA GGTGGTGCTG
    ATCAATGCAG TGAAAGATGT GGCCAAGGCC CTGGGAGACC TCATCAGTGC CACAAAGGCT
    GCGGCTGGCA AAGTCGGCGA CGACCCTGCT GTGTGGCAGC TCAAGAACTC TGCCAAGGTG
    ATGGTAACCA ATGTGACATC ACTGCTCAAG ACCGTGAAAG CGGTAGAGGA TGAGGCCACC
    AAAGGCACGC GCGCCCTGGA GGCCACAACG GAGCATATTC GGCAGGAGCT GGCGGTCTTC
    TGCTCCCCAG AGCCACCTGC CAAGACCTCG ACCCCTGAAG ATTTCATCCG CATGACCAAG
    GGTATCACCA TGGCAACCGC CAAGGCCGTT GCCGCTGGGA ACTCCTGTCG CCAGGAGGAT
    GTCATTGCCA CAGCCAATCT GAGTCGCCGT GCTATTGCAG ATATGCTCCG GGCTTGCAAG
    GAAGCTGCCT ACCACCCAGA AGTGGCTCCA GACGTGCGGC TCCGAGCCCT ACACTTTGGC
    CGGGAATGTG CCAATGGCTA CCTGGAACTG CTGGACCACG TGCTGCTGAC CCTGCAGAAG
    CCAAGCCCAG AATTGAAGCA GCAATTGACG GTGCACTCAA AGCGTGTGGC TGGATCAGTC
    ACTGAGCTCA TCCAGGCTGC TGAGGCCATG AAGGGAACAG AATGGGTGGA CCCAGAGGAC
    CCCACAGTCA TTGCTGAGAA TGAACTCCTG GGAGCCGCGG CTGCCATTGA GGCTGCGGCC
    AAGAAGCTGG AGCAGCTGAA GCCCCGGGCC AAACCTAAGG AGGCGGACGA GTCCTTGAAC
    TTTGAGGAGC AGATACTGGA AGCTGCCAAG TCCATCGCAG CAGCCACCAG CGCCCTGGTG
    AAGGCTGCCT CAGCTGCACA GAGGGAACTG GTGGCCCAGG GGAAGGTGGG CGCCATTCCA
    GCCAACGCAC TGGATGATGG ACAGTGGTCC CAGGGCCTCA TCTCTGCTGC CCGGATGGTG
    GCTGCGGCCA CCAACAACCT GTGTGAGGCA GCCAATGCAG CTGTCCAAGG CCACGCCAGC
    CAGGAGAAGC TCATCTCATC AGCCAAGCAG GTCGCCGCCT CCACAGCCCA GCTCCTCGTA
    GCCTGCAAGG TCAAGGCCGA CCAGGACTCT GAGGCAATGA AGCGGCTTCA GGCTGCCGGC
    AACGCAGTGA AGCGAGCCTC AGACAACCTG GTGAAGGCAG CCCAGAAGGC TGCAGCCTTT
    GAAGAACAGG AGAATGAGAC CGTGGTGGTG AAGGAGAAGA TGGTCGGGGG CATTGCCCAG
    ATCATTGCTG CCCAGGAAGA GATGCTTCGG AAGGAACGAG AGCTGGAGGA GGCACGGAAG
    AAGCTGGCGC AGATCCGGCA GCAGCAGTAC AAATTCTTAC CTTCAGAGCT TCGAGACGAG
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237295
    Clone ID Related Accession (Same CDS sequence) XM_005210126.3 , XM_010807953.2
    Accession Version XM_005210126.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7677bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product talin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210126.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTTGCAC TTTCGCTGAA GATCAGCATT GGGAATGTGG TGAAGACGAT GCAATTTGAG 
    CCGTCTACCA TGGTGTATGA CGCCTGTCGG ATCATCCGTG AGCGGATCCC AGAGGCCCTA
    TCTGGCCCTC CCAGCGACTT TGGGCTGTTT CTGTCAGATG ATGACCCCAA AAAGGGTATA
    TGGCTGGAGG CAGGGAAGGC TTTGGATTAT TACATGCTCC GAAACGGGGA CACCATGGAG
    TATAGGAAGA AACAGAGGCC CTTGAAGATC CGCATGCTGG ACGGCACCGT GAAGACTGTC
    ATGGTGGACG ACTCGAAGAC TGTCACGGAC ATGCTCATGA CCATCTGTGC CCGCATTGGC
    ATCACCAACC ATGATGAATA TTCACTGGTT CGAGAGATTA TGGAAGAGAA GAAGGAGGAG
    GTGACAGGGA CCTTACGAAA GGACAAGACA CTGCTGCGAG ACGAAAAGAA GATGGAGAAA
    CTGAAGCAGA AGTTGCACAC AGATGACGAG TTGAACTGGC TGGACCATGG CCGGACCCTG
    AGGGAGCAGG GAGTGGAGGA GCACGAAACG CTTCTGCTCC GGAGGAAGTT CTTCTACTCA
    GATCAGAACG TGGACTCCCG GGACCCCGTC CAGCTGAACC TTCTCTACGT GCAGGCACGA
    GATGACATCC TGAATGGCTC CCATCCTGTC TCCTTCGACA AGGCCTGTGA GTTTGCTGGC
    TTCCAATGCC AGATCCAGTT TGGGCCCCAC AATGAGCAGA AGCACAAGGC CGGCTTCCTC
    GACCTGAAGG ACTTCCTGCC CAAGGAGTAT GTGAAGCAGA AGGGAGAGCG TAAGATCTTC
    CAGGCACACA AGAACTGTGG ACAGATGAGT GAGATTGAGG CCAAGGTACG CTACGTGAAG
    CTAGCCCGTT CCCTCAAGAC GTACGGTGTC TCCTTCTTCC TGGTCAAGGA AAAGATGAAA
    GGAAAGAACA AGTTGGTGCC CAGGCTTCTG GGCATCACTA AGGAGTGTGT GATGCGAGTG
    GACGAGAAGA CCAAGGAGGT GATCCAGGAA TGGAACCTCA CCAACATAAA ACGCTGGGCT
    GCGTCTCCCA AGAGCTTCAC CCTGGATTTC GGAGACTACC AAGATGGCTA CTACTCGGTG
    CAGACGACCG AGGGGGAGCA GATCGCTCAG CTCATCGCTG GCTACATCGA CATCATCCTT
    AAGAAGAAAA AAAGCAAGGA TCATTTTGGG CTGGAGGGAG ACGAGGAGTC TACTATGCTG
    GAGGACTCCG TGTCCCCCAA AAAGTCCACT GTGCTTCAGC AGCAGTATAA CCGGGTGGGG
    AAGGTGGAGC ATGGCTCCGT GGCCCTGCCC GCCATCATGC GCTCCGGAGC CTCGGGTCCC
    GAGAATTTCC AGGTGGGCAG CATGCCACCT GCCCAGCAGC AGATCACCAG TGGCCAGATG
    CACCGAGGAC ACATGCCTCC TTTGACGTCA GCCCAGCAGG CGCTCACTGG GACCATCAAC
    TCCAGCATGC AGGCTGTGCA AGCTGCCCAG GCCACACTGG ATGACTTTGA CACTCTGCCC
    CCTCTGGGCC AGGACGCTGC CTCAAAGGCC TGGCGTAAGA ACAAGATGGA TGAATCCAAG
    CATGAAATCC ACTCCCAGGT AGACGCCATC ACCGCTGGTA CCGCCTCTGT GGTGAACCTC
    ACAGCAGGGG ACCCTGCTGA GACAGACTAC ACGGCAGTGG GCTGTGCGGT CACCACCATC
    TCCTCCAACC TGACGGAGAT GTCCCGTGGC GTGAAGCTGC TTGCTGCCCT GCTGGAGGAT
    GAAGGCGGCA GCGGCCGGCC CCTGCTGCAG GCGGCCAAGG GCCTGGCGGG GGCGGTGTCA
    GAGCTGCTGC GCAGTGCCCA GCCAGCCAGT GCCGAGCCCC GTCAGAACCT GCTGCAAGCA
    GCTGGGAACG TGGGCCAGGC CAGTGGGGAG CTGTTGCAAC AAATTGGGGA AAGTGATACT
    GACCCCCACT TCCAGATCTG TGCACCCAGC GGGGCTGGGT TTCCATCTCC CCCCGATCCC
    CCCACGGACG TGCTCATGCA GCTAGCCAAG GCCGTGGCAA GCGCTGCAGC TGCCCTGGTC
    CTCAAGGCCA AGAGTGTGGC CCAGCGAACA GAGGACTCAG GGCTTCAGAC CCAGGTCATC
    GCTGCGGCAA CACAGTGTGC CCTGTCCACC TCCCAGCTGG TGGCCTGTAC CAAGGTGGTG
    GCTCCTACTA TCAGCTCCCC AGTCTGCCAA GAACAGCTGG TAGAGGCTGG GCGACTAGTG
    GCCAAGGCTG TGGAGGGCTG TGTGTCCGCT TCCCAGGCCG CCACAGAGGA TGCCCAGCTG
    TTACGGGGGG TAGGTGCTGC GGCCACAGCT GTCACCCAGG CCCTGAATGA GCTGCTGCAG
    CACGTGAGGG CCCATGCCAC AGGGGCTGGG CCTGCTGGCC GCTACGACCA GGCCACTGAC
    ACCATCCTCA CTGTCACCGA GAACATCTTC AGCTCCATGG GTGATGCTGG TGAGATGGTG
    CGACAGGCCC GCATCCTCGC CCAAGCCACC TCTGACCTGG TGAACGCCAT CAAGGCTGAT
    GCCGAAGGGG AGAGTGATCT GGAGAATTCC CGCAAGCTGT TAAGTGCCGC CAAGATCCTG
    GCCGATGCCA CAGCCAAGAT GGTGGAGGCT GCCAAGGGAG CAGCCGCCAA TCCTGACAGT
    GAGGAGCAAC AGCAGCGGCT GCGGGAGGCA GCAGAGGGTC TCCGCATGGC AACCAATGCC
    GCTGCACAGA ACGCCATCAA GAAAAAGCTG GTGCAGCGCC TTGAGCATGC AGCCAAACAG
    GCTGCAGCCT CGGCTACACA GACTATCGCA GCAGCCCAAC ATGCAACCTC CACTCCCAAG
    GCCTCTGCTG GCCCCCAGCC CCTGCTGGTG CAGAGCTGCA AGGCTGTGGC AGAGCAGATT
    CCACTGCTGG TGCAGGGCGT CCGAGGGAGC CAAGCGCAGC CTGACAGCCC CAGTGCTCAG
    CTAGCCCTCA TCGCTGCCAG CCAAAGCTTC CTGCAGCCAG GTGGGAAGAT GGTGGCAGCC
    GCAAAGGCCT CTGTGCCAAC AATTCAGGAC CAAGCATCGG CCATGCAGCT GAGTCAGTGT
    GCTAAAAACC TTGGCACCGC GTTGGCTGAA CTCCGTACTG CTGCCCAGAA GGCTCAGGAA
    GCGTGTGGGC CTTTGGAAAT GGATTCTGCT CTGAGTGTGG TGCAGAATCT GGAGAGAGAC
    CTGCAGGAAG TGAAGGCAGC TGCTCGAGAC GGCAAGCTGA AGCCCTTGCC TGGGGAGACG
    ATGGAGAAAT GTGCCCAGGA CCTGGGCAAC AGCACCAAAG CCGTGAGCTC CGCTATTGCC
    CAGCTGCTGG GGGAGGTCGC CCAGGGCAAT GAGAATTACG CAGGCATTGC AGCTCGGGAT
    GTGGCAGGTG GGCTGCGGTC ACTGGCCCAA GCTGCTAGGG GCGTAGCTGC CCTGACATCA
    GATCCTGCAG TGCAAGCCAT TGTGCTCGAC ACAGCCAGCG ATGTCCTGGA CAAGGCCAGC
    AGCCTCATCG AGGAGGCAAA GAAAGCAGCC GGCCATCCAG GGGACCCTGA GAGCCAGCAG
    CGGCTTGCCC AGGTGGCTAA AGCTGTGACC CAGGCCCTGA ACCGTTGCGT CAGCTGCCTG
    CCTGGCCAGC GTGATGTGGA CAATGCCCTG AGGGCAGTGG GAGATGCCAG CAAGCGACTC
    CTGAGTGACT CGCTTCCCCC CAGCACTGGG ACCTTTCAGG AGGCTCAGAG CCGGCTGAAT
    GAAGCCGCTG CCGGGCTGAA TCAGGCAGCC ACAGAGCTGG TGCAGGCCTC TCGGGGAACC
    CCTCAGGACC TGGCCCGAGC CTCAGGTCGA TTTGGACAAG ACTTCAGCAC CTTCCTGGAA
    GCCGGCGTGG AGATGGCAGG CCAGGCTCCG AGCCAGGAGG ACCGTGCCCA GGTCGTATCC
    AACTTGAAGG GCATCTCCAT GTCTTCAAGC AAACTTCTTC TCGCTGCCAA GGCCCTGTCC
    ACAGACCCTG CAGCCCCAAC CCTCAAGAGT CAGCTGGCTG CAGCTGCCCG GGCAGTGACC
    GACAGCATCA ACCAGCTCAT CAGCATGTGC ACCCAGCAGG CGCCAGGCCA GAAGGAGTGT
    GATAATGCCC TGCGGGAATT GGAGACGGTC CGGGAACTCC TAGAGAACCC AGTCCAGCCC
    ATCAATGACA TGTCCTACTT TGGCTGTCTG GACAGCGTCA TGGAAAACTC AAAGGTGCTG
    GGTGAGGCCA TGACCGGCAT CTCCCAAAAT GCCAAGAACG GAAACTTGCC GGAGTTTGGG
    GAGGCTGTCG CCACAGCCTC CAAGGCCCTC TGCGGCTTCA CCGAGGCAGC TGCACAGGCT
    GCATATCTGG TTGGCGTGTC TGACCCCAAC AGCCAAGCTG GACAACAAGG GCTGGTGGAG
    CCCACACAGT TTGCCCGAGC AAACCAGGCA ATTCAGATGG CCTGTCAGAG TTTGGGGGAG
    CCTGGCTGCA CCCAGGCCCA GGTGCTCTCT GCAGCCACCA TTGTGGCCAA ACACACGTCT
    GCGCTGTGTA ACAGCTGCCG CCTGGCTTCT GCCCGAACCG CCAATCCCAC TGCCAAGCGC
    CAGTTCGTAC AGTCAGCCAA GGAGGTGGCC AACAGCACGG CCAACCTTGT CAAGACCATC
    AAGGCGCTGG ATGGGGCCTT CACAGAGGAG AACCGTGCCC AGTGCCGAGC AGCAACGGCC
    CCTTTGCTGG AGGCTGTGGA CAATCTGAGC GCCTTTGCGT CCAACCCTGA GTTCTCCAGT
    GTTCCTGCCC AGATCAGCCC TGAGGGCCGG GCTGCCATGG AGCCCATTGT GATCTCTGCT
    AAGACAATGT TGGAGAGTGC TGGGGGCCTT ATTCAGACAG CCCGAGCCCT GGCGGTCAAT
    CCGCGGGACC CACCACGCTG GTCCGTGCTG GCTGGCCACT CCCGTACTGT CTCAGACTCC
    ATCAAGAAGC TGATCACAAG CATGAGGGAC AAGGCCCCAG GGCAGTTGGA GTGTGAGACA
    GCCATCGCAG CTCTGAACAG CTGTCTGCGG GACCTTGACC AGGCTTCTCT TGCTGCAGTC
    AGCCAGCAGC TCGCTCCCCG TGAGGGGATC TCTCAGGAGG CCTTGCACAC CCAGATGCTC
    ACTGCGGTGC AGGAAATCTC CCATCTTATT GAGCCGCTGG CCAGCGCTGC CCGGGCTGAA
    GCCTCCCAGC TGGGACACAA GGTGTCCCAG ATGGCCCAGT ACTTTGAGCC GCTCACTCTG
    GCTGCCGTGG GTGCTGCCTC CAAAACCCTG AGCCACCCAC AGCAGATGGC GCTCCTGGAC
    CAGACCAAAA CCTTGGCCGA GTCTGCCCTG CAGCTGCTAT ACACAGCCAA GGAAGCTGGC
    GGTAACCCCA AGCAAGCAGC TCACACCCAG GAAGCCCTGG AGGAGGCTGT GCAGATGATG
    ACAGAGGCCG TAGAGGACCT GACAACCACC CTCAACGAGG CAGCCAGTGC TGCCGGGGTC
    GTCGGTGGCA TGGTGGACTC CATCACCCAG GCCATCAACC AGCTAGATGA AGGACCAATG
    GGTGAACCAG AGGGTTCCTT CGTGGATTAC CAGACCACCA TGGTGCGGAC AGCCAAGGCC
    ATTGCTGTCA CCGTTCAGGA GATGGTAACC AAGTCAAACA CCAGCCCTGA GGAGCTGGGC
    CCTCTCGCTA ACCAGCTGAC TAGTGACTAT GGGCGTCTTG CCTCCCAGGC CAAGCCTGCA
    GCTGTGGCTG CTGAAAATGA AGAGATAGGC GCCCACATCA AGCACCGGGT TCAGGAGCTG
    GGGCACGGCT GCGCAGCTCT GGTCACCAAG GCAGGCGCCC TGCAGTGTAG CCCTAGTGAT
    GCCTACACCA AGAAGGAGCT CATAGAGTGT GCCCGGAGAG TCTCTGAGAA GGTCTCCCAC
    GTGCTGGCTG CGCTCCAGGC TGGGAATCGT GGCACCCAGG CCTGCATCAC AGCCGCCAGC
    GCTGTGTCCG GCATCATCGC CGACCTGGAC ACCACCATCA TGTTCGCCAC TGCCGGCACA
    CTCAACCGTG AGGGTGCCGA AACCTTTGCT GACCACCGAG AGGGCATTCT GAAGACTGCC
    AAGGTGCTGG TGGAAGACAC CAAGGTCCTG GTGCAGAATG CAGCTGGGAG CCAGGAGAAG
    CTGGCACAGG CAGCCCAGTC CTCCGTGGCC ACCATCACCC GCCTCGCCGA TGTGGTCAAG
    CTGGGTGCAG CCAGCCTGGG AGCTGAGGAC CCAGAGACCC AGGTGGTGCT GATCAATGCA
    GTGAAAGATG TGGCCAAGGC CCTGGGAGAC CTCATCAGTG CCACAAAGGC TGCGGCTGGC
    AAAGTCGGCG ACGACCCTGC TGTGTGGCAG CTCAAGAACT CTGCCAAGGT GATGGTAACC
    AATGTGACAT CACTGCTCAA GACCGTGAAA GCGGTAGAGG ATGAGGCCAC CAAAGGCACG
    CGCGCCCTGG AGGCCACAAC GGAGCATATT CGGCAGGAGC TGGCGGTCTT CTGCTCCCCA
    GAGCCACCTG CCAAGACCTC GACCCCTGAA GATTTCATCC GCATGACCAA GGGTATCACC
    ATGGCAACCG CCAAGGCCGT TGCCGCTGGG AACTCCTGTC GCCAGGAGGA TGTCATTGCC
    ACAGCCAATC TGAGTCGCCG TGCTATTGCA GATATGCTCC GGGCTTGCAA GGAAGCTGCC
    TACCACCCAG AAGTGGCTCC AGACGTGCGG CTCCGAGCCC TACACTTTGG CCGGGAATGT
    GCCAATGGCT ACCTGGAACT GCTGGACCAC GTGCTGCTGA CCCTGCAGAA GCCAAGCCCA
    GAATTGAAGC AGCAATTGAC GGTGCACTCA AAGCGTGTGG CTGGATCAGT CACTGAGCTC
    ATCCAGGCTG CTGAGGCCAT GAAGGGAACA GAATGGGTGG ACCCAGAGGA CCCCACAGTC
    ATTGCTGAGA ATGAACTCCT GGGAGCCGCG GCTGCCATTG AGGCTGCGGC CAAGAAGCTG
    GAGCAGCTGA AGCCCCGGGC CAAACCTAAG GAGGCGGACG AGTCCTTGAA CTTTGAGGAG
    CAGATACTGG AAGCTGCCAA GTCCATCGCA GCAGCCACCA GCGCCCTGGT GAAGGCTGCC
    TCAGCTGCAC AGAGGGAACT GGTGGCCCAG GGGAAGGTGG GCGCCATTCC AGCCAACGCA
    CTGGATGATG GACAGTGGTC CCAGGGCCTC ATCTCTGCTG CCCGGATGGT GGCTGCGGCC
    ACCAACAACC TGTGTGAGGC AGCCAATGCA GCTGTCCAAG GCCACGCCAG CCAGGAGAAG
    CTCATCTCAT CAGCCAAGCA GGTCGCCGCC TCCACAGCCC AGCTCCTCGT AGCCTGCAAG
    GTCAAGGCCG ACCAGGACTC TGAGGCAATG AAGCGGCTTC AGGCTGCCGG CAACGCAGTG
    AAGCGAGCCT CAGACAACCT GGTGAAGGCA GCCCAGAAGG CTGCAGCCTT TGAAGAACAG
    GAGAATGAGA CCGTGGTGGT GAAGGAGAAG ATGGTCGGGG GCATTGCCCA GATCATTGCT
    GCCCAGGAAG AGATGCTTCG GAAGGAACGA GAGCTGGAGG AGGCACGGAA GAAGCTGGCG
    CAGATCCGGC AGCAGCAGTA CAAATTCTTA CCTTCAGAGC TTCGAGACGA GCACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210183.2
    CDS314..7990
    Translation

    Target ORF information:

    RefSeq Version XM_005210126.3
    Organism Bos taurus(cattle)
    Definition Bos taurus talin 1 (TLN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210126.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTTGCAC TTTCGCTGAA GATCAGCATT GGGAATGTGG TGAAGACGAT GCAATTTGAG 
    CCGTCTACCA TGGTGTATGA CGCCTGTCGG ATCATCCGTG AGCGGATCCC AGAGGCCCTA
    TCTGGCCCTC CCAGCGACTT TGGGCTGTTT CTGTCAGATG ATGACCCCAA AAAGGGTATA
    TGGCTGGAGG CAGGGAAGGC TTTGGATTAT TACATGCTCC GAAACGGGGA CACCATGGAG
    TATAGGAAGA AACAGAGGCC CTTGAAGATC CGCATGCTGG ACGGCACCGT GAAGACTGTC
    ATGGTGGACG ACTCGAAGAC TGTCACGGAC ATGCTCATGA CCATCTGTGC CCGCATTGGC
    ATCACCAACC ATGATGAATA TTCACTGGTT CGAGAGATTA TGGAAGAGAA GAAGGAGGAG
    GTGACAGGGA CCTTACGAAA GGACAAGACA CTGCTGCGAG ACGAAAAGAA GATGGAGAAA
    CTGAAGCAGA AGTTGCACAC AGATGACGAG TTGAACTGGC TGGACCATGG CCGGACCCTG
    AGGGAGCAGG GAGTGGAGGA GCACGAAACG CTTCTGCTCC GGAGGAAGTT CTTCTACTCA
    GATCAGAACG TGGACTCCCG GGACCCCGTC CAGCTGAACC TTCTCTACGT GCAGGCACGA
    GATGACATCC TGAATGGCTC CCATCCTGTC TCCTTCGACA AGGCCTGTGA GTTTGCTGGC
    TTCCAATGCC AGATCCAGTT TGGGCCCCAC AATGAGCAGA AGCACAAGGC CGGCTTCCTC
    GACCTGAAGG ACTTCCTGCC CAAGGAGTAT GTGAAGCAGA AGGGAGAGCG TAAGATCTTC
    CAGGCACACA AGAACTGTGG ACAGATGAGT GAGATTGAGG CCAAGGTACG CTACGTGAAG
    CTAGCCCGTT CCCTCAAGAC GTACGGTGTC TCCTTCTTCC TGGTCAAGGA AAAGATGAAA
    GGAAAGAACA AGTTGGTGCC CAGGCTTCTG GGCATCACTA AGGAGTGTGT GATGCGAGTG
    GACGAGAAGA CCAAGGAGGT GATCCAGGAA TGGAACCTCA CCAACATAAA ACGCTGGGCT
    GCGTCTCCCA AGAGCTTCAC CCTGGATTTC GGAGACTACC AAGATGGCTA CTACTCGGTG
    CAGACGACCG AGGGGGAGCA GATCGCTCAG CTCATCGCTG GCTACATCGA CATCATCCTT
    AAGAAGAAAA AAAGCAAGGA TCATTTTGGG CTGGAGGGAG ACGAGGAGTC TACTATGCTG
    GAGGACTCCG TGTCCCCCAA AAAGTCCACT GTGCTTCAGC AGCAGTATAA CCGGGTGGGG
    AAGGTGGAGC ATGGCTCCGT GGCCCTGCCC GCCATCATGC GCTCCGGAGC CTCGGGTCCC
    GAGAATTTCC AGGTGGGCAG CATGCCACCT GCCCAGCAGC AGATCACCAG TGGCCAGATG
    CACCGAGGAC ACATGCCTCC TTTGACGTCA GCCCAGCAGG CGCTCACTGG GACCATCAAC
    TCCAGCATGC AGGCTGTGCA AGCTGCCCAG GCCACACTGG ATGACTTTGA CACTCTGCCC
    CCTCTGGGCC AGGACGCTGC CTCAAAGGCC TGGCGTAAGA ACAAGATGGA TGAATCCAAG
    CATGAAATCC ACTCCCAGGT AGACGCCATC ACCGCTGGTA CCGCCTCTGT GGTGAACCTC
    ACAGCAGGGG ACCCTGCTGA GACAGACTAC ACGGCAGTGG GCTGTGCGGT CACCACCATC
    TCCTCCAACC TGACGGAGAT GTCCCGTGGC GTGAAGCTGC TTGCTGCCCT GCTGGAGGAT
    GAAGGCGGCA GCGGCCGGCC CCTGCTGCAG GCGGCCAAGG GCCTGGCGGG GGCGGTGTCA
    GAGCTGCTGC GCAGTGCCCA GCCAGCCAGT GCCGAGCCCC GTCAGAACCT GCTGCAAGCA
    GCTGGGAACG TGGGCCAGGC CAGTGGGGAG CTGTTGCAAC AAATTGGGGA AAGTGATACT
    GACCCCCACT TCCAGATCTG TGCACCCAGC GGGGCTGGGT TTCCATCTCC CCCCGATCCC
    CCCACGGACG TGCTCATGCA GCTAGCCAAG GCCGTGGCAA GCGCTGCAGC TGCCCTGGTC
    CTCAAGGCCA AGAGTGTGGC CCAGCGAACA GAGGACTCAG GGCTTCAGAC CCAGGTCATC
    GCTGCGGCAA CACAGTGTGC CCTGTCCACC TCCCAGCTGG TGGCCTGTAC CAAGGTGGTG
    GCTCCTACTA TCAGCTCCCC AGTCTGCCAA GAACAGCTGG TAGAGGCTGG GCGACTAGTG
    GCCAAGGCTG TGGAGGGCTG TGTGTCCGCT TCCCAGGCCG CCACAGAGGA TGCCCAGCTG
    TTACGGGGGG TAGGTGCTGC GGCCACAGCT GTCACCCAGG CCCTGAATGA GCTGCTGCAG
    CACGTGAGGG CCCATGCCAC AGGGGCTGGG CCTGCTGGCC GCTACGACCA GGCCACTGAC
    ACCATCCTCA CTGTCACCGA GAACATCTTC AGCTCCATGG GTGATGCTGG TGAGATGGTG
    CGACAGGCCC GCATCCTCGC CCAAGCCACC TCTGACCTGG TGAACGCCAT CAAGGCTGAT
    GCCGAAGGGG AGAGTGATCT GGAGAATTCC CGCAAGCTGT TAAGTGCCGC CAAGATCCTG
    GCCGATGCCA CAGCCAAGAT GGTGGAGGCT GCCAAGGGAG CAGCCGCCAA TCCTGACAGT
    GAGGAGCAAC AGCAGCGGCT GCGGGAGGCA GCAGAGGGTC TCCGCATGGC AACCAATGCC
    GCTGCACAGA ACGCCATCAA GAAAAAGCTG GTGCAGCGCC TTGAGCATGC AGCCAAACAG
    GCTGCAGCCT CGGCTACACA GACTATCGCA GCAGCCCAAC ATGCAACCTC CACTCCCAAG
    GCCTCTGCTG GCCCCCAGCC CCTGCTGGTG CAGAGCTGCA AGGCTGTGGC AGAGCAGATT
    CCACTGCTGG TGCAGGGCGT CCGAGGGAGC CAAGCGCAGC CTGACAGCCC CAGTGCTCAG
    CTAGCCCTCA TCGCTGCCAG CCAAAGCTTC CTGCAGCCAG GTGGGAAGAT GGTGGCAGCC
    GCAAAGGCCT CTGTGCCAAC AATTCAGGAC CAAGCATCGG CCATGCAGCT GAGTCAGTGT
    GCTAAAAACC TTGGCACCGC GTTGGCTGAA CTCCGTACTG CTGCCCAGAA GGCTCAGGAA
    GCGTGTGGGC CTTTGGAAAT GGATTCTGCT CTGAGTGTGG TGCAGAATCT GGAGAGAGAC
    CTGCAGGAAG TGAAGGCAGC TGCTCGAGAC GGCAAGCTGA AGCCCTTGCC TGGGGAGACG
    ATGGAGAAAT GTGCCCAGGA CCTGGGCAAC AGCACCAAAG CCGTGAGCTC CGCTATTGCC
    CAGCTGCTGG GGGAGGTCGC CCAGGGCAAT GAGAATTACG CAGGCATTGC AGCTCGGGAT
    GTGGCAGGTG GGCTGCGGTC ACTGGCCCAA GCTGCTAGGG GCGTAGCTGC CCTGACATCA
    GATCCTGCAG TGCAAGCCAT TGTGCTCGAC ACAGCCAGCG ATGTCCTGGA CAAGGCCAGC
    AGCCTCATCG AGGAGGCAAA GAAAGCAGCC GGCCATCCAG GGGACCCTGA GAGCCAGCAG
    CGGCTTGCCC AGGTGGCTAA AGCTGTGACC CAGGCCCTGA ACCGTTGCGT CAGCTGCCTG
    CCTGGCCAGC GTGATGTGGA CAATGCCCTG AGGGCAGTGG GAGATGCCAG CAAGCGACTC
    CTGAGTGACT CGCTTCCCCC CAGCACTGGG ACCTTTCAGG AGGCTCAGAG CCGGCTGAAT
    GAAGCCGCTG CCGGGCTGAA TCAGGCAGCC ACAGAGCTGG TGCAGGCCTC TCGGGGAACC
    CCTCAGGACC TGGCCCGAGC CTCAGGTCGA TTTGGACAAG ACTTCAGCAC CTTCCTGGAA
    GCCGGCGTGG AGATGGCAGG CCAGGCTCCG AGCCAGGAGG ACCGTGCCCA GGTCGTATCC
    AACTTGAAGG GCATCTCCAT GTCTTCAAGC AAACTTCTTC TCGCTGCCAA GGCCCTGTCC
    ACAGACCCTG CAGCCCCAAC CCTCAAGAGT CAGCTGGCTG CAGCTGCCCG GGCAGTGACC
    GACAGCATCA ACCAGCTCAT CAGCATGTGC ACCCAGCAGG CGCCAGGCCA GAAGGAGTGT
    GATAATGCCC TGCGGGAATT GGAGACGGTC CGGGAACTCC TAGAGAACCC AGTCCAGCCC
    ATCAATGACA TGTCCTACTT TGGCTGTCTG GACAGCGTCA TGGAAAACTC AAAGGTGCTG
    GGTGAGGCCA TGACCGGCAT CTCCCAAAAT GCCAAGAACG GAAACTTGCC GGAGTTTGGG
    GAGGCTGTCG CCACAGCCTC CAAGGCCCTC TGCGGCTTCA CCGAGGCAGC TGCACAGGCT
    GCATATCTGG TTGGCGTGTC TGACCCCAAC AGCCAAGCTG GACAACAAGG GCTGGTGGAG
    CCCACACAGT TTGCCCGAGC AAACCAGGCA ATTCAGATGG CCTGTCAGAG TTTGGGGGAG
    CCTGGCTGCA CCCAGGCCCA GGTGCTCTCT GCAGCCACCA TTGTGGCCAA ACACACGTCT
    GCGCTGTGTA ACAGCTGCCG CCTGGCTTCT GCCCGAACCG CCAATCCCAC TGCCAAGCGC
    CAGTTCGTAC AGTCAGCCAA GGAGGTGGCC AACAGCACGG CCAACCTTGT CAAGACCATC
    AAGGCGCTGG ATGGGGCCTT CACAGAGGAG AACCGTGCCC AGTGCCGAGC AGCAACGGCC
    CCTTTGCTGG AGGCTGTGGA CAATCTGAGC GCCTTTGCGT CCAACCCTGA GTTCTCCAGT
    GTTCCTGCCC AGATCAGCCC TGAGGGCCGG GCTGCCATGG AGCCCATTGT GATCTCTGCT
    AAGACAATGT TGGAGAGTGC TGGGGGCCTT ATTCAGACAG CCCGAGCCCT GGCGGTCAAT
    CCGCGGGACC CACCACGCTG GTCCGTGCTG GCTGGCCACT CCCGTACTGT CTCAGACTCC
    ATCAAGAAGC TGATCACAAG CATGAGGGAC AAGGCCCCAG GGCAGTTGGA GTGTGAGACA
    GCCATCGCAG CTCTGAACAG CTGTCTGCGG GACCTTGACC AGGCTTCTCT TGCTGCAGTC
    AGCCAGCAGC TCGCTCCCCG TGAGGGGATC TCTCAGGAGG CCTTGCACAC CCAGATGCTC
    ACTGCGGTGC AGGAAATCTC CCATCTTATT GAGCCGCTGG CCAGCGCTGC CCGGGCTGAA
    GCCTCCCAGC TGGGACACAA GGTGTCCCAG ATGGCCCAGT ACTTTGAGCC GCTCACTCTG
    GCTGCCGTGG GTGCTGCCTC CAAAACCCTG AGCCACCCAC AGCAGATGGC GCTCCTGGAC
    CAGACCAAAA CCTTGGCCGA GTCTGCCCTG CAGCTGCTAT ACACAGCCAA GGAAGCTGGC
    GGTAACCCCA AGCAAGCAGC TCACACCCAG GAAGCCCTGG AGGAGGCTGT GCAGATGATG
    ACAGAGGCCG TAGAGGACCT GACAACCACC CTCAACGAGG CAGCCAGTGC TGCCGGGGTC
    GTCGGTGGCA TGGTGGACTC CATCACCCAG GCCATCAACC AGCTAGATGA AGGACCAATG
    GGTGAACCAG AGGGTTCCTT CGTGGATTAC CAGACCACCA TGGTGCGGAC AGCCAAGGCC
    ATTGCTGTCA CCGTTCAGGA GATGGTAACC AAGTCAAACA CCAGCCCTGA GGAGCTGGGC
    CCTCTCGCTA ACCAGCTGAC TAGTGACTAT GGGCGTCTTG CCTCCCAGGC CAAGCCTGCA
    GCTGTGGCTG CTGAAAATGA AGAGATAGGC GCCCACATCA AGCACCGGGT TCAGGAGCTG
    GGGCACGGCT GCGCAGCTCT GGTCACCAAG GCAGGCGCCC TGCAGTGTAG CCCTAGTGAT
    GCCTACACCA AGAAGGAGCT CATAGAGTGT GCCCGGAGAG TCTCTGAGAA GGTCTCCCAC
    GTGCTGGCTG CGCTCCAGGC TGGGAATCGT GGCACCCAGG CCTGCATCAC AGCCGCCAGC
    GCTGTGTCCG GCATCATCGC CGACCTGGAC ACCACCATCA TGTTCGCCAC TGCCGGCACA
    CTCAACCGTG AGGGTGCCGA AACCTTTGCT GACCACCGAG AGGGCATTCT GAAGACTGCC
    AAGGTGCTGG TGGAAGACAC CAAGGTCCTG GTGCAGAATG CAGCTGGGAG CCAGGAGAAG
    CTGGCACAGG CAGCCCAGTC CTCCGTGGCC ACCATCACCC GCCTCGCCGA TGTGGTCAAG
    CTGGGTGCAG CCAGCCTGGG AGCTGAGGAC CCAGAGACCC AGGTGGTGCT GATCAATGCA
    GTGAAAGATG TGGCCAAGGC CCTGGGAGAC CTCATCAGTG CCACAAAGGC TGCGGCTGGC
    AAAGTCGGCG ACGACCCTGC TGTGTGGCAG CTCAAGAACT CTGCCAAGGT GATGGTAACC
    AATGTGACAT CACTGCTCAA GACCGTGAAA GCGGTAGAGG ATGAGGCCAC CAAAGGCACG
    CGCGCCCTGG AGGCCACAAC GGAGCATATT CGGCAGGAGC TGGCGGTCTT CTGCTCCCCA
    GAGCCACCTG CCAAGACCTC GACCCCTGAA GATTTCATCC GCATGACCAA GGGTATCACC
    ATGGCAACCG CCAAGGCCGT TGCCGCTGGG AACTCCTGTC GCCAGGAGGA TGTCATTGCC
    ACAGCCAATC TGAGTCGCCG TGCTATTGCA GATATGCTCC GGGCTTGCAA GGAAGCTGCC
    TACCACCCAG AAGTGGCTCC AGACGTGCGG CTCCGAGCCC TACACTTTGG CCGGGAATGT
    GCCAATGGCT ACCTGGAACT GCTGGACCAC GTGCTGCTGA CCCTGCAGAA GCCAAGCCCA
    GAATTGAAGC AGCAATTGAC GGTGCACTCA AAGCGTGTGG CTGGATCAGT CACTGAGCTC
    ATCCAGGCTG CTGAGGCCAT GAAGGGAACA GAATGGGTGG ACCCAGAGGA CCCCACAGTC
    ATTGCTGAGA ATGAACTCCT GGGAGCCGCG GCTGCCATTG AGGCTGCGGC CAAGAAGCTG
    GAGCAGCTGA AGCCCCGGGC CAAACCTAAG GAGGCGGACG AGTCCTTGAA CTTTGAGGAG
    CAGATACTGG AAGCTGCCAA GTCCATCGCA GCAGCCACCA GCGCCCTGGT GAAGGCTGCC
    TCAGCTGCAC AGAGGGAACT GGTGGCCCAG GGGAAGGTGG GCGCCATTCC AGCCAACGCA
    CTGGATGATG GACAGTGGTC CCAGGGCCTC ATCTCTGCTG CCCGGATGGT GGCTGCGGCC
    ACCAACAACC TGTGTGAGGC AGCCAATGCA GCTGTCCAAG GCCACGCCAG CCAGGAGAAG
    CTCATCTCAT CAGCCAAGCA GGTCGCCGCC TCCACAGCCC AGCTCCTCGT AGCCTGCAAG
    GTCAAGGCCG ACCAGGACTC TGAGGCAATG AAGCGGCTTC AGGCTGCCGG CAACGCAGTG
    AAGCGAGCCT CAGACAACCT GGTGAAGGCA GCCCAGAAGG CTGCAGCCTT TGAAGAACAG
    GAGAATGAGA CCGTGGTGGT GAAGGAGAAG ATGGTCGGGG GCATTGCCCA GATCATTGCT
    GCCCAGGAAG AGATGCTTCG GAAGGAACGA GAGCTGGAGG AGGCACGGAA GAAGCTGGCG
    CAGATCCGGC AGCAGCAGTA CAAATTCTTA CCTTCAGAGC TTCGAGACGA GCACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237295
    Clone ID Related Accession (Same CDS sequence) XM_005210126.3 , XM_010807953.2
    Accession Version XM_010807953.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7677bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product talin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807953.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTTGCAC TTTCGCTGAA GATCAGCATT GGGAATGTGG TGAAGACGAT GCAATTTGAG 
    CCGTCTACCA TGGTGTATGA CGCCTGTCGG ATCATCCGTG AGCGGATCCC AGAGGCCCTA
    TCTGGCCCTC CCAGCGACTT TGGGCTGTTT CTGTCAGATG ATGACCCCAA AAAGGGTATA
    TGGCTGGAGG CAGGGAAGGC TTTGGATTAT TACATGCTCC GAAACGGGGA CACCATGGAG
    TATAGGAAGA AACAGAGGCC CTTGAAGATC CGCATGCTGG ACGGCACCGT GAAGACTGTC
    ATGGTGGACG ACTCGAAGAC TGTCACGGAC ATGCTCATGA CCATCTGTGC CCGCATTGGC
    ATCACCAACC ATGATGAATA TTCACTGGTT CGAGAGATTA TGGAAGAGAA GAAGGAGGAG
    GTGACAGGGA CCTTACGAAA GGACAAGACA CTGCTGCGAG ACGAAAAGAA GATGGAGAAA
    CTGAAGCAGA AGTTGCACAC AGATGACGAG TTGAACTGGC TGGACCATGG CCGGACCCTG
    AGGGAGCAGG GAGTGGAGGA GCACGAAACG CTTCTGCTCC GGAGGAAGTT CTTCTACTCA
    GATCAGAACG TGGACTCCCG GGACCCCGTC CAGCTGAACC TTCTCTACGT GCAGGCACGA
    GATGACATCC TGAATGGCTC CCATCCTGTC TCCTTCGACA AGGCCTGTGA GTTTGCTGGC
    TTCCAATGCC AGATCCAGTT TGGGCCCCAC AATGAGCAGA AGCACAAGGC CGGCTTCCTC
    GACCTGAAGG ACTTCCTGCC CAAGGAGTAT GTGAAGCAGA AGGGAGAGCG TAAGATCTTC
    CAGGCACACA AGAACTGTGG ACAGATGAGT GAGATTGAGG CCAAGGTACG CTACGTGAAG
    CTAGCCCGTT CCCTCAAGAC GTACGGTGTC TCCTTCTTCC TGGTCAAGGA AAAGATGAAA
    GGAAAGAACA AGTTGGTGCC CAGGCTTCTG GGCATCACTA AGGAGTGTGT GATGCGAGTG
    GACGAGAAGA CCAAGGAGGT GATCCAGGAA TGGAACCTCA CCAACATAAA ACGCTGGGCT
    GCGTCTCCCA AGAGCTTCAC CCTGGATTTC GGAGACTACC AAGATGGCTA CTACTCGGTG
    CAGACGACCG AGGGGGAGCA GATCGCTCAG CTCATCGCTG GCTACATCGA CATCATCCTT
    AAGAAGAAAA AAAGCAAGGA TCATTTTGGG CTGGAGGGAG ACGAGGAGTC TACTATGCTG
    GAGGACTCCG TGTCCCCCAA AAAGTCCACT GTGCTTCAGC AGCAGTATAA CCGGGTGGGG
    AAGGTGGAGC ATGGCTCCGT GGCCCTGCCC GCCATCATGC GCTCCGGAGC CTCGGGTCCC
    GAGAATTTCC AGGTGGGCAG CATGCCACCT GCCCAGCAGC AGATCACCAG TGGCCAGATG
    CACCGAGGAC ACATGCCTCC TTTGACGTCA GCCCAGCAGG CGCTCACTGG GACCATCAAC
    TCCAGCATGC AGGCTGTGCA AGCTGCCCAG GCCACACTGG ATGACTTTGA CACTCTGCCC
    CCTCTGGGCC AGGACGCTGC CTCAAAGGCC TGGCGTAAGA ACAAGATGGA TGAATCCAAG
    CATGAAATCC ACTCCCAGGT AGACGCCATC ACCGCTGGTA CCGCCTCTGT GGTGAACCTC
    ACAGCAGGGG ACCCTGCTGA GACAGACTAC ACGGCAGTGG GCTGTGCGGT CACCACCATC
    TCCTCCAACC TGACGGAGAT GTCCCGTGGC GTGAAGCTGC TTGCTGCCCT GCTGGAGGAT
    GAAGGCGGCA GCGGCCGGCC CCTGCTGCAG GCGGCCAAGG GCCTGGCGGG GGCGGTGTCA
    GAGCTGCTGC GCAGTGCCCA GCCAGCCAGT GCCGAGCCCC GTCAGAACCT GCTGCAAGCA
    GCTGGGAACG TGGGCCAGGC CAGTGGGGAG CTGTTGCAAC AAATTGGGGA AAGTGATACT
    GACCCCCACT TCCAGATCTG TGCACCCAGC GGGGCTGGGT TTCCATCTCC CCCCGATCCC
    CCCACGGACG TGCTCATGCA GCTAGCCAAG GCCGTGGCAA GCGCTGCAGC TGCCCTGGTC
    CTCAAGGCCA AGAGTGTGGC CCAGCGAACA GAGGACTCAG GGCTTCAGAC CCAGGTCATC
    GCTGCGGCAA CACAGTGTGC CCTGTCCACC TCCCAGCTGG TGGCCTGTAC CAAGGTGGTG
    GCTCCTACTA TCAGCTCCCC AGTCTGCCAA GAACAGCTGG TAGAGGCTGG GCGACTAGTG
    GCCAAGGCTG TGGAGGGCTG TGTGTCCGCT TCCCAGGCCG CCACAGAGGA TGCCCAGCTG
    TTACGGGGGG TAGGTGCTGC GGCCACAGCT GTCACCCAGG CCCTGAATGA GCTGCTGCAG
    CACGTGAGGG CCCATGCCAC AGGGGCTGGG CCTGCTGGCC GCTACGACCA GGCCACTGAC
    ACCATCCTCA CTGTCACCGA GAACATCTTC AGCTCCATGG GTGATGCTGG TGAGATGGTG
    CGACAGGCCC GCATCCTCGC CCAAGCCACC TCTGACCTGG TGAACGCCAT CAAGGCTGAT
    GCCGAAGGGG AGAGTGATCT GGAGAATTCC CGCAAGCTGT TAAGTGCCGC CAAGATCCTG
    GCCGATGCCA CAGCCAAGAT GGTGGAGGCT GCCAAGGGAG CAGCCGCCAA TCCTGACAGT
    GAGGAGCAAC AGCAGCGGCT GCGGGAGGCA GCAGAGGGTC TCCGCATGGC AACCAATGCC
    GCTGCACAGA ACGCCATCAA GAAAAAGCTG GTGCAGCGCC TTGAGCATGC AGCCAAACAG
    GCTGCAGCCT CGGCTACACA GACTATCGCA GCAGCCCAAC ATGCAACCTC CACTCCCAAG
    GCCTCTGCTG GCCCCCAGCC CCTGCTGGTG CAGAGCTGCA AGGCTGTGGC AGAGCAGATT
    CCACTGCTGG TGCAGGGCGT CCGAGGGAGC CAAGCGCAGC CTGACAGCCC CAGTGCTCAG
    CTAGCCCTCA TCGCTGCCAG CCAAAGCTTC CTGCAGCCAG GTGGGAAGAT GGTGGCAGCC
    GCAAAGGCCT CTGTGCCAAC AATTCAGGAC CAAGCATCGG CCATGCAGCT GAGTCAGTGT
    GCTAAAAACC TTGGCACCGC GTTGGCTGAA CTCCGTACTG CTGCCCAGAA GGCTCAGGAA
    GCGTGTGGGC CTTTGGAAAT GGATTCTGCT CTGAGTGTGG TGCAGAATCT GGAGAGAGAC
    CTGCAGGAAG TGAAGGCAGC TGCTCGAGAC GGCAAGCTGA AGCCCTTGCC TGGGGAGACG
    ATGGAGAAAT GTGCCCAGGA CCTGGGCAAC AGCACCAAAG CCGTGAGCTC CGCTATTGCC
    CAGCTGCTGG GGGAGGTCGC CCAGGGCAAT GAGAATTACG CAGGCATTGC AGCTCGGGAT
    GTGGCAGGTG GGCTGCGGTC ACTGGCCCAA GCTGCTAGGG GCGTAGCTGC CCTGACATCA
    GATCCTGCAG TGCAAGCCAT TGTGCTCGAC ACAGCCAGCG ATGTCCTGGA CAAGGCCAGC
    AGCCTCATCG AGGAGGCAAA GAAAGCAGCC GGCCATCCAG GGGACCCTGA GAGCCAGCAG
    CGGCTTGCCC AGGTGGCTAA AGCTGTGACC CAGGCCCTGA ACCGTTGCGT CAGCTGCCTG
    CCTGGCCAGC GTGATGTGGA CAATGCCCTG AGGGCAGTGG GAGATGCCAG CAAGCGACTC
    CTGAGTGACT CGCTTCCCCC CAGCACTGGG ACCTTTCAGG AGGCTCAGAG CCGGCTGAAT
    GAAGCCGCTG CCGGGCTGAA TCAGGCAGCC ACAGAGCTGG TGCAGGCCTC TCGGGGAACC
    CCTCAGGACC TGGCCCGAGC CTCAGGTCGA TTTGGACAAG ACTTCAGCAC CTTCCTGGAA
    GCCGGCGTGG AGATGGCAGG CCAGGCTCCG AGCCAGGAGG ACCGTGCCCA GGTCGTATCC
    AACTTGAAGG GCATCTCCAT GTCTTCAAGC AAACTTCTTC TCGCTGCCAA GGCCCTGTCC
    ACAGACCCTG CAGCCCCAAC CCTCAAGAGT CAGCTGGCTG CAGCTGCCCG GGCAGTGACC
    GACAGCATCA ACCAGCTCAT CAGCATGTGC ACCCAGCAGG CGCCAGGCCA GAAGGAGTGT
    GATAATGCCC TGCGGGAATT GGAGACGGTC CGGGAACTCC TAGAGAACCC AGTCCAGCCC
    ATCAATGACA TGTCCTACTT TGGCTGTCTG GACAGCGTCA TGGAAAACTC AAAGGTGCTG
    GGTGAGGCCA TGACCGGCAT CTCCCAAAAT GCCAAGAACG GAAACTTGCC GGAGTTTGGG
    GAGGCTGTCG CCACAGCCTC CAAGGCCCTC TGCGGCTTCA CCGAGGCAGC TGCACAGGCT
    GCATATCTGG TTGGCGTGTC TGACCCCAAC AGCCAAGCTG GACAACAAGG GCTGGTGGAG
    CCCACACAGT TTGCCCGAGC AAACCAGGCA ATTCAGATGG CCTGTCAGAG TTTGGGGGAG
    CCTGGCTGCA CCCAGGCCCA GGTGCTCTCT GCAGCCACCA TTGTGGCCAA ACACACGTCT
    GCGCTGTGTA ACAGCTGCCG CCTGGCTTCT GCCCGAACCG CCAATCCCAC TGCCAAGCGC
    CAGTTCGTAC AGTCAGCCAA GGAGGTGGCC AACAGCACGG CCAACCTTGT CAAGACCATC
    AAGGCGCTGG ATGGGGCCTT CACAGAGGAG AACCGTGCCC AGTGCCGAGC AGCAACGGCC
    CCTTTGCTGG AGGCTGTGGA CAATCTGAGC GCCTTTGCGT CCAACCCTGA GTTCTCCAGT
    GTTCCTGCCC AGATCAGCCC TGAGGGCCGG GCTGCCATGG AGCCCATTGT GATCTCTGCT
    AAGACAATGT TGGAGAGTGC TGGGGGCCTT ATTCAGACAG CCCGAGCCCT GGCGGTCAAT
    CCGCGGGACC CACCACGCTG GTCCGTGCTG GCTGGCCACT CCCGTACTGT CTCAGACTCC
    ATCAAGAAGC TGATCACAAG CATGAGGGAC AAGGCCCCAG GGCAGTTGGA GTGTGAGACA
    GCCATCGCAG CTCTGAACAG CTGTCTGCGG GACCTTGACC AGGCTTCTCT TGCTGCAGTC
    AGCCAGCAGC TCGCTCCCCG TGAGGGGATC TCTCAGGAGG CCTTGCACAC CCAGATGCTC
    ACTGCGGTGC AGGAAATCTC CCATCTTATT GAGCCGCTGG CCAGCGCTGC CCGGGCTGAA
    GCCTCCCAGC TGGGACACAA GGTGTCCCAG ATGGCCCAGT ACTTTGAGCC GCTCACTCTG
    GCTGCCGTGG GTGCTGCCTC CAAAACCCTG AGCCACCCAC AGCAGATGGC GCTCCTGGAC
    CAGACCAAAA CCTTGGCCGA GTCTGCCCTG CAGCTGCTAT ACACAGCCAA GGAAGCTGGC
    GGTAACCCCA AGCAAGCAGC TCACACCCAG GAAGCCCTGG AGGAGGCTGT GCAGATGATG
    ACAGAGGCCG TAGAGGACCT GACAACCACC CTCAACGAGG CAGCCAGTGC TGCCGGGGTC
    GTCGGTGGCA TGGTGGACTC CATCACCCAG GCCATCAACC AGCTAGATGA AGGACCAATG
    GGTGAACCAG AGGGTTCCTT CGTGGATTAC CAGACCACCA TGGTGCGGAC AGCCAAGGCC
    ATTGCTGTCA CCGTTCAGGA GATGGTAACC AAGTCAAACA CCAGCCCTGA GGAGCTGGGC
    CCTCTCGCTA ACCAGCTGAC TAGTGACTAT GGGCGTCTTG CCTCCCAGGC CAAGCCTGCA
    GCTGTGGCTG CTGAAAATGA AGAGATAGGC GCCCACATCA AGCACCGGGT TCAGGAGCTG
    GGGCACGGCT GCGCAGCTCT GGTCACCAAG GCAGGCGCCC TGCAGTGTAG CCCTAGTGAT
    GCCTACACCA AGAAGGAGCT CATAGAGTGT GCCCGGAGAG TCTCTGAGAA GGTCTCCCAC
    GTGCTGGCTG CGCTCCAGGC TGGGAATCGT GGCACCCAGG CCTGCATCAC AGCCGCCAGC
    GCTGTGTCCG GCATCATCGC CGACCTGGAC ACCACCATCA TGTTCGCCAC TGCCGGCACA
    CTCAACCGTG AGGGTGCCGA AACCTTTGCT GACCACCGAG AGGGCATTCT GAAGACTGCC
    AAGGTGCTGG TGGAAGACAC CAAGGTCCTG GTGCAGAATG CAGCTGGGAG CCAGGAGAAG
    CTGGCACAGG CAGCCCAGTC CTCCGTGGCC ACCATCACCC GCCTCGCCGA TGTGGTCAAG
    CTGGGTGCAG CCAGCCTGGG AGCTGAGGAC CCAGAGACCC AGGTGGTGCT GATCAATGCA
    GTGAAAGATG TGGCCAAGGC CCTGGGAGAC CTCATCAGTG CCACAAAGGC TGCGGCTGGC
    AAAGTCGGCG ACGACCCTGC TGTGTGGCAG CTCAAGAACT CTGCCAAGGT GATGGTAACC
    AATGTGACAT CACTGCTCAA GACCGTGAAA GCGGTAGAGG ATGAGGCCAC CAAAGGCACG
    CGCGCCCTGG AGGCCACAAC GGAGCATATT CGGCAGGAGC TGGCGGTCTT CTGCTCCCCA
    GAGCCACCTG CCAAGACCTC GACCCCTGAA GATTTCATCC GCATGACCAA GGGTATCACC
    ATGGCAACCG CCAAGGCCGT TGCCGCTGGG AACTCCTGTC GCCAGGAGGA TGTCATTGCC
    ACAGCCAATC TGAGTCGCCG TGCTATTGCA GATATGCTCC GGGCTTGCAA GGAAGCTGCC
    TACCACCCAG AAGTGGCTCC AGACGTGCGG CTCCGAGCCC TACACTTTGG CCGGGAATGT
    GCCAATGGCT ACCTGGAACT GCTGGACCAC GTGCTGCTGA CCCTGCAGAA GCCAAGCCCA
    GAATTGAAGC AGCAATTGAC GGTGCACTCA AAGCGTGTGG CTGGATCAGT CACTGAGCTC
    ATCCAGGCTG CTGAGGCCAT GAAGGGAACA GAATGGGTGG ACCCAGAGGA CCCCACAGTC
    ATTGCTGAGA ATGAACTCCT GGGAGCCGCG GCTGCCATTG AGGCTGCGGC CAAGAAGCTG
    GAGCAGCTGA AGCCCCGGGC CAAACCTAAG GAGGCGGACG AGTCCTTGAA CTTTGAGGAG
    CAGATACTGG AAGCTGCCAA GTCCATCGCA GCAGCCACCA GCGCCCTGGT GAAGGCTGCC
    TCAGCTGCAC AGAGGGAACT GGTGGCCCAG GGGAAGGTGG GCGCCATTCC AGCCAACGCA
    CTGGATGATG GACAGTGGTC CCAGGGCCTC ATCTCTGCTG CCCGGATGGT GGCTGCGGCC
    ACCAACAACC TGTGTGAGGC AGCCAATGCA GCTGTCCAAG GCCACGCCAG CCAGGAGAAG
    CTCATCTCAT CAGCCAAGCA GGTCGCCGCC TCCACAGCCC AGCTCCTCGT AGCCTGCAAG
    GTCAAGGCCG ACCAGGACTC TGAGGCAATG AAGCGGCTTC AGGCTGCCGG CAACGCAGTG
    AAGCGAGCCT CAGACAACCT GGTGAAGGCA GCCCAGAAGG CTGCAGCCTT TGAAGAACAG
    GAGAATGAGA CCGTGGTGGT GAAGGAGAAG ATGGTCGGGG GCATTGCCCA GATCATTGCT
    GCCCAGGAAG AGATGCTTCG GAAGGAACGA GAGCTGGAGG AGGCACGGAA GAAGCTGGCG
    CAGATCCGGC AGCAGCAGTA CAAATTCTTA CCTTCAGAGC TTCGAGACGA GCACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806255.2
    CDS455..8131
    Translation

    Target ORF information:

    RefSeq Version XM_010807953.2
    Organism Bos taurus(cattle)
    Definition Bos taurus talin 1 (TLN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807953.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTTGCAC TTTCGCTGAA GATCAGCATT GGGAATGTGG TGAAGACGAT GCAATTTGAG 
    CCGTCTACCA TGGTGTATGA CGCCTGTCGG ATCATCCGTG AGCGGATCCC AGAGGCCCTA
    TCTGGCCCTC CCAGCGACTT TGGGCTGTTT CTGTCAGATG ATGACCCCAA AAAGGGTATA
    TGGCTGGAGG CAGGGAAGGC TTTGGATTAT TACATGCTCC GAAACGGGGA CACCATGGAG
    TATAGGAAGA AACAGAGGCC CTTGAAGATC CGCATGCTGG ACGGCACCGT GAAGACTGTC
    ATGGTGGACG ACTCGAAGAC TGTCACGGAC ATGCTCATGA CCATCTGTGC CCGCATTGGC
    ATCACCAACC ATGATGAATA TTCACTGGTT CGAGAGATTA TGGAAGAGAA GAAGGAGGAG
    GTGACAGGGA CCTTACGAAA GGACAAGACA CTGCTGCGAG ACGAAAAGAA GATGGAGAAA
    CTGAAGCAGA AGTTGCACAC AGATGACGAG TTGAACTGGC TGGACCATGG CCGGACCCTG
    AGGGAGCAGG GAGTGGAGGA GCACGAAACG CTTCTGCTCC GGAGGAAGTT CTTCTACTCA
    GATCAGAACG TGGACTCCCG GGACCCCGTC CAGCTGAACC TTCTCTACGT GCAGGCACGA
    GATGACATCC TGAATGGCTC CCATCCTGTC TCCTTCGACA AGGCCTGTGA GTTTGCTGGC
    TTCCAATGCC AGATCCAGTT TGGGCCCCAC AATGAGCAGA AGCACAAGGC CGGCTTCCTC
    GACCTGAAGG ACTTCCTGCC CAAGGAGTAT GTGAAGCAGA AGGGAGAGCG TAAGATCTTC
    CAGGCACACA AGAACTGTGG ACAGATGAGT GAGATTGAGG CCAAGGTACG CTACGTGAAG
    CTAGCCCGTT CCCTCAAGAC GTACGGTGTC TCCTTCTTCC TGGTCAAGGA AAAGATGAAA
    GGAAAGAACA AGTTGGTGCC CAGGCTTCTG GGCATCACTA AGGAGTGTGT GATGCGAGTG
    GACGAGAAGA CCAAGGAGGT GATCCAGGAA TGGAACCTCA CCAACATAAA ACGCTGGGCT
    GCGTCTCCCA AGAGCTTCAC CCTGGATTTC GGAGACTACC AAGATGGCTA CTACTCGGTG
    CAGACGACCG AGGGGGAGCA GATCGCTCAG CTCATCGCTG GCTACATCGA CATCATCCTT
    AAGAAGAAAA AAAGCAAGGA TCATTTTGGG CTGGAGGGAG ACGAGGAGTC TACTATGCTG
    GAGGACTCCG TGTCCCCCAA AAAGTCCACT GTGCTTCAGC AGCAGTATAA CCGGGTGGGG
    AAGGTGGAGC ATGGCTCCGT GGCCCTGCCC GCCATCATGC GCTCCGGAGC CTCGGGTCCC
    GAGAATTTCC AGGTGGGCAG CATGCCACCT GCCCAGCAGC AGATCACCAG TGGCCAGATG
    CACCGAGGAC ACATGCCTCC TTTGACGTCA GCCCAGCAGG CGCTCACTGG GACCATCAAC
    TCCAGCATGC AGGCTGTGCA AGCTGCCCAG GCCACACTGG ATGACTTTGA CACTCTGCCC
    CCTCTGGGCC AGGACGCTGC CTCAAAGGCC TGGCGTAAGA ACAAGATGGA TGAATCCAAG
    CATGAAATCC ACTCCCAGGT AGACGCCATC ACCGCTGGTA CCGCCTCTGT GGTGAACCTC
    ACAGCAGGGG ACCCTGCTGA GACAGACTAC ACGGCAGTGG GCTGTGCGGT CACCACCATC
    TCCTCCAACC TGACGGAGAT GTCCCGTGGC GTGAAGCTGC TTGCTGCCCT GCTGGAGGAT
    GAAGGCGGCA GCGGCCGGCC CCTGCTGCAG GCGGCCAAGG GCCTGGCGGG GGCGGTGTCA
    GAGCTGCTGC GCAGTGCCCA GCCAGCCAGT GCCGAGCCCC GTCAGAACCT GCTGCAAGCA
    GCTGGGAACG TGGGCCAGGC CAGTGGGGAG CTGTTGCAAC AAATTGGGGA AAGTGATACT
    GACCCCCACT TCCAGATCTG TGCACCCAGC GGGGCTGGGT TTCCATCTCC CCCCGATCCC
    CCCACGGACG TGCTCATGCA GCTAGCCAAG GCCGTGGCAA GCGCTGCAGC TGCCCTGGTC
    CTCAAGGCCA AGAGTGTGGC CCAGCGAACA GAGGACTCAG GGCTTCAGAC CCAGGTCATC
    GCTGCGGCAA CACAGTGTGC CCTGTCCACC TCCCAGCTGG TGGCCTGTAC CAAGGTGGTG
    GCTCCTACTA TCAGCTCCCC AGTCTGCCAA GAACAGCTGG TAGAGGCTGG GCGACTAGTG
    GCCAAGGCTG TGGAGGGCTG TGTGTCCGCT TCCCAGGCCG CCACAGAGGA TGCCCAGCTG
    TTACGGGGGG TAGGTGCTGC GGCCACAGCT GTCACCCAGG CCCTGAATGA GCTGCTGCAG
    CACGTGAGGG CCCATGCCAC AGGGGCTGGG CCTGCTGGCC GCTACGACCA GGCCACTGAC
    ACCATCCTCA CTGTCACCGA GAACATCTTC AGCTCCATGG GTGATGCTGG TGAGATGGTG
    CGACAGGCCC GCATCCTCGC CCAAGCCACC TCTGACCTGG TGAACGCCAT CAAGGCTGAT
    GCCGAAGGGG AGAGTGATCT GGAGAATTCC CGCAAGCTGT TAAGTGCCGC CAAGATCCTG
    GCCGATGCCA CAGCCAAGAT GGTGGAGGCT GCCAAGGGAG CAGCCGCCAA TCCTGACAGT
    GAGGAGCAAC AGCAGCGGCT GCGGGAGGCA GCAGAGGGTC TCCGCATGGC AACCAATGCC
    GCTGCACAGA ACGCCATCAA GAAAAAGCTG GTGCAGCGCC TTGAGCATGC AGCCAAACAG
    GCTGCAGCCT CGGCTACACA GACTATCGCA GCAGCCCAAC ATGCAACCTC CACTCCCAAG
    GCCTCTGCTG GCCCCCAGCC CCTGCTGGTG CAGAGCTGCA AGGCTGTGGC AGAGCAGATT
    CCACTGCTGG TGCAGGGCGT CCGAGGGAGC CAAGCGCAGC CTGACAGCCC CAGTGCTCAG
    CTAGCCCTCA TCGCTGCCAG CCAAAGCTTC CTGCAGCCAG GTGGGAAGAT GGTGGCAGCC
    GCAAAGGCCT CTGTGCCAAC AATTCAGGAC CAAGCATCGG CCATGCAGCT GAGTCAGTGT
    GCTAAAAACC TTGGCACCGC GTTGGCTGAA CTCCGTACTG CTGCCCAGAA GGCTCAGGAA
    GCGTGTGGGC CTTTGGAAAT GGATTCTGCT CTGAGTGTGG TGCAGAATCT GGAGAGAGAC
    CTGCAGGAAG TGAAGGCAGC TGCTCGAGAC GGCAAGCTGA AGCCCTTGCC TGGGGAGACG
    ATGGAGAAAT GTGCCCAGGA CCTGGGCAAC AGCACCAAAG CCGTGAGCTC CGCTATTGCC
    CAGCTGCTGG GGGAGGTCGC CCAGGGCAAT GAGAATTACG CAGGCATTGC AGCTCGGGAT
    GTGGCAGGTG GGCTGCGGTC ACTGGCCCAA GCTGCTAGGG GCGTAGCTGC CCTGACATCA
    GATCCTGCAG TGCAAGCCAT TGTGCTCGAC ACAGCCAGCG ATGTCCTGGA CAAGGCCAGC
    AGCCTCATCG AGGAGGCAAA GAAAGCAGCC GGCCATCCAG GGGACCCTGA GAGCCAGCAG
    CGGCTTGCCC AGGTGGCTAA AGCTGTGACC CAGGCCCTGA ACCGTTGCGT CAGCTGCCTG
    CCTGGCCAGC GTGATGTGGA CAATGCCCTG AGGGCAGTGG GAGATGCCAG CAAGCGACTC
    CTGAGTGACT CGCTTCCCCC CAGCACTGGG ACCTTTCAGG AGGCTCAGAG CCGGCTGAAT
    GAAGCCGCTG CCGGGCTGAA TCAGGCAGCC ACAGAGCTGG TGCAGGCCTC TCGGGGAACC
    CCTCAGGACC TGGCCCGAGC CTCAGGTCGA TTTGGACAAG ACTTCAGCAC CTTCCTGGAA
    GCCGGCGTGG AGATGGCAGG CCAGGCTCCG AGCCAGGAGG ACCGTGCCCA GGTCGTATCC
    AACTTGAAGG GCATCTCCAT GTCTTCAAGC AAACTTCTTC TCGCTGCCAA GGCCCTGTCC
    ACAGACCCTG CAGCCCCAAC CCTCAAGAGT CAGCTGGCTG CAGCTGCCCG GGCAGTGACC
    GACAGCATCA ACCAGCTCAT CAGCATGTGC ACCCAGCAGG CGCCAGGCCA GAAGGAGTGT
    GATAATGCCC TGCGGGAATT GGAGACGGTC CGGGAACTCC TAGAGAACCC AGTCCAGCCC
    ATCAATGACA TGTCCTACTT TGGCTGTCTG GACAGCGTCA TGGAAAACTC AAAGGTGCTG
    GGTGAGGCCA TGACCGGCAT CTCCCAAAAT GCCAAGAACG GAAACTTGCC GGAGTTTGGG
    GAGGCTGTCG CCACAGCCTC CAAGGCCCTC TGCGGCTTCA CCGAGGCAGC TGCACAGGCT
    GCATATCTGG TTGGCGTGTC TGACCCCAAC AGCCAAGCTG GACAACAAGG GCTGGTGGAG
    CCCACACAGT TTGCCCGAGC AAACCAGGCA ATTCAGATGG CCTGTCAGAG TTTGGGGGAG
    CCTGGCTGCA CCCAGGCCCA GGTGCTCTCT GCAGCCACCA TTGTGGCCAA ACACACGTCT
    GCGCTGTGTA ACAGCTGCCG CCTGGCTTCT GCCCGAACCG CCAATCCCAC TGCCAAGCGC
    CAGTTCGTAC AGTCAGCCAA GGAGGTGGCC AACAGCACGG CCAACCTTGT CAAGACCATC
    AAGGCGCTGG ATGGGGCCTT CACAGAGGAG AACCGTGCCC AGTGCCGAGC AGCAACGGCC
    CCTTTGCTGG AGGCTGTGGA CAATCTGAGC GCCTTTGCGT CCAACCCTGA GTTCTCCAGT
    GTTCCTGCCC AGATCAGCCC TGAGGGCCGG GCTGCCATGG AGCCCATTGT GATCTCTGCT
    AAGACAATGT TGGAGAGTGC TGGGGGCCTT ATTCAGACAG CCCGAGCCCT GGCGGTCAAT
    CCGCGGGACC CACCACGCTG GTCCGTGCTG GCTGGCCACT CCCGTACTGT CTCAGACTCC
    ATCAAGAAGC TGATCACAAG CATGAGGGAC AAGGCCCCAG GGCAGTTGGA GTGTGAGACA
    GCCATCGCAG CTCTGAACAG CTGTCTGCGG GACCTTGACC AGGCTTCTCT TGCTGCAGTC
    AGCCAGCAGC TCGCTCCCCG TGAGGGGATC TCTCAGGAGG CCTTGCACAC CCAGATGCTC
    ACTGCGGTGC AGGAAATCTC CCATCTTATT GAGCCGCTGG CCAGCGCTGC CCGGGCTGAA
    GCCTCCCAGC TGGGACACAA GGTGTCCCAG ATGGCCCAGT ACTTTGAGCC GCTCACTCTG
    GCTGCCGTGG GTGCTGCCTC CAAAACCCTG AGCCACCCAC AGCAGATGGC GCTCCTGGAC
    CAGACCAAAA CCTTGGCCGA GTCTGCCCTG CAGCTGCTAT ACACAGCCAA GGAAGCTGGC
    GGTAACCCCA AGCAAGCAGC TCACACCCAG GAAGCCCTGG AGGAGGCTGT GCAGATGATG
    ACAGAGGCCG TAGAGGACCT GACAACCACC CTCAACGAGG CAGCCAGTGC TGCCGGGGTC
    GTCGGTGGCA TGGTGGACTC CATCACCCAG GCCATCAACC AGCTAGATGA AGGACCAATG
    GGTGAACCAG AGGGTTCCTT CGTGGATTAC CAGACCACCA TGGTGCGGAC AGCCAAGGCC
    ATTGCTGTCA CCGTTCAGGA GATGGTAACC AAGTCAAACA CCAGCCCTGA GGAGCTGGGC
    CCTCTCGCTA ACCAGCTGAC TAGTGACTAT GGGCGTCTTG CCTCCCAGGC CAAGCCTGCA
    GCTGTGGCTG CTGAAAATGA AGAGATAGGC GCCCACATCA AGCACCGGGT TCAGGAGCTG
    GGGCACGGCT GCGCAGCTCT GGTCACCAAG GCAGGCGCCC TGCAGTGTAG CCCTAGTGAT
    GCCTACACCA AGAAGGAGCT CATAGAGTGT GCCCGGAGAG TCTCTGAGAA GGTCTCCCAC
    GTGCTGGCTG CGCTCCAGGC TGGGAATCGT GGCACCCAGG CCTGCATCAC AGCCGCCAGC
    GCTGTGTCCG GCATCATCGC CGACCTGGAC ACCACCATCA TGTTCGCCAC TGCCGGCACA
    CTCAACCGTG AGGGTGCCGA AACCTTTGCT GACCACCGAG AGGGCATTCT GAAGACTGCC
    AAGGTGCTGG TGGAAGACAC CAAGGTCCTG GTGCAGAATG CAGCTGGGAG CCAGGAGAAG
    CTGGCACAGG CAGCCCAGTC CTCCGTGGCC ACCATCACCC GCCTCGCCGA TGTGGTCAAG
    CTGGGTGCAG CCAGCCTGGG AGCTGAGGAC CCAGAGACCC AGGTGGTGCT GATCAATGCA
    GTGAAAGATG TGGCCAAGGC CCTGGGAGAC CTCATCAGTG CCACAAAGGC TGCGGCTGGC
    AAAGTCGGCG ACGACCCTGC TGTGTGGCAG CTCAAGAACT CTGCCAAGGT GATGGTAACC
    AATGTGACAT CACTGCTCAA GACCGTGAAA GCGGTAGAGG ATGAGGCCAC CAAAGGCACG
    CGCGCCCTGG AGGCCACAAC GGAGCATATT CGGCAGGAGC TGGCGGTCTT CTGCTCCCCA
    GAGCCACCTG CCAAGACCTC GACCCCTGAA GATTTCATCC GCATGACCAA GGGTATCACC
    ATGGCAACCG CCAAGGCCGT TGCCGCTGGG AACTCCTGTC GCCAGGAGGA TGTCATTGCC
    ACAGCCAATC TGAGTCGCCG TGCTATTGCA GATATGCTCC GGGCTTGCAA GGAAGCTGCC
    TACCACCCAG AAGTGGCTCC AGACGTGCGG CTCCGAGCCC TACACTTTGG CCGGGAATGT
    GCCAATGGCT ACCTGGAACT GCTGGACCAC GTGCTGCTGA CCCTGCAGAA GCCAAGCCCA
    GAATTGAAGC AGCAATTGAC GGTGCACTCA AAGCGTGTGG CTGGATCAGT CACTGAGCTC
    ATCCAGGCTG CTGAGGCCAT GAAGGGAACA GAATGGGTGG ACCCAGAGGA CCCCACAGTC
    ATTGCTGAGA ATGAACTCCT GGGAGCCGCG GCTGCCATTG AGGCTGCGGC CAAGAAGCTG
    GAGCAGCTGA AGCCCCGGGC CAAACCTAAG GAGGCGGACG AGTCCTTGAA CTTTGAGGAG
    CAGATACTGG AAGCTGCCAA GTCCATCGCA GCAGCCACCA GCGCCCTGGT GAAGGCTGCC
    TCAGCTGCAC AGAGGGAACT GGTGGCCCAG GGGAAGGTGG GCGCCATTCC AGCCAACGCA
    CTGGATGATG GACAGTGGTC CCAGGGCCTC ATCTCTGCTG CCCGGATGGT GGCTGCGGCC
    ACCAACAACC TGTGTGAGGC AGCCAATGCA GCTGTCCAAG GCCACGCCAG CCAGGAGAAG
    CTCATCTCAT CAGCCAAGCA GGTCGCCGCC TCCACAGCCC AGCTCCTCGT AGCCTGCAAG
    GTCAAGGCCG ACCAGGACTC TGAGGCAATG AAGCGGCTTC AGGCTGCCGG CAACGCAGTG
    AAGCGAGCCT CAGACAACCT GGTGAAGGCA GCCCAGAAGG CTGCAGCCTT TGAAGAACAG
    GAGAATGAGA CCGTGGTGGT GAAGGAGAAG ATGGTCGGGG GCATTGCCCA GATCATTGCT
    GCCCAGGAAG AGATGCTTCG GAAGGAACGA GAGCTGGAGG AGGCACGGAA GAAGCTGGCG
    CAGATCCGGC AGCAGCAGTA CAAATTCTTA CCTTCAGAGC TTCGAGACGA GCACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237296
    Clone ID Related Accession (Same CDS sequence) NM_001205428.3 , XM_024995995.1
    Accession Version XM_024995995.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product talin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTTGCAC TTTCGCTGAA GATCAGCATT GGGAATGTGG TGAAGACGAT GCAATTTGAG 
    CCGTCTACCA TGGTGTATGA CGCCTGTCGG ATCATCCGTG AGCGGATCCC AGAGGCCCTA
    TCTGGCCCTC CCAGCGACTT TGGGCTGTTT CTGTCAGATG ATGACCCCAA AAAGGGTATA
    TGGCTGGAGG CAGGGAAGGC TTTGGATTAT TACATGCTCC GAAACGGGGA CACCATGGAG
    TATAGGAAGA AACAGAGGCC CTTGAAGATC CGCATGCTGG ACGGCACCGT GAAGACTGTC
    ATGGTGGACG ACTCGAAGAC TGTCACGGAC ATGCTCATGA CCATCTGTGC CCGCATTGGC
    ATCACCAACC ATGATGAATA TTCACTGGTT CGAGAGATTA TGGAAGAGAA GAAGGAGGAG
    GTGACAGGGA CCTTACGAAA GGACAAGACA CTGCTGCGAG ACGAAAAGAA GATGGAGAAA
    CTGAAGCAGA AGTTGCACAC AGATGACGAG TTGAACTGGC TGGACCATGG CCGGACCCTG
    AGGGAGCAGG GAGTGGAGGA GCACGAAACG CTTCTGCTCC GGAGGAAGTT CTTCTACTCA
    GATCAGAACG TGGACTCCCG GGACCCCGTC CAGCTGAACC TTCTCTACGT GCAGGCACGA
    GATGACATCC TGAATGGCTC CCATCCTGTC TCCTTCGACA AGGCCTGTGA GTTTGCTGGC
    TTCCAATGCC AGATCCAGTT TGGGCCCCAC AATGAGCAGA AGCACAAGGC CGGCTTCCTC
    GACCTGAAGG ACTTCCTGCC CAAGGAGTAT GTGAAGCAGA AGGGAGAGCG TAAGATCTTC
    CAGGCACACA AGAACTGTGG ACAGATGAGT GAGATTGAGG CCAAGGTACG CTACGTGAAG
    CTAGCCCGTT CCCTCAAGAC GTACGGTGTC TCCTTCTTCC TGGTCAAGGA AAAGATGAAA
    GGAAAGAACA AGTTGGTGCC CAGGCTTCTG GGCATCACTA AGGAGTGTGT GATGCGAGTG
    GACGAGAAGA CCAAGGAGGT GATCCAGGAA TGGAACCTCA CCAACATAAA ACGCTGGGCT
    GCGTCTCCCA AGAGCTTCAC CCTGGATTTC GGAGACTACC AAGATGGCTA CTACTCGGTG
    CAGACGACCG AGGGGGAGCA GATCGCTCAG CTCATCGCTG GCTACATCGA CATCATCCTT
    AAGAAGAAAA AAAGCAAGGA TCATTTTGGG CTGGAGGGAG ACGAGGAGTC TACTATGCTG
    GAGGACTCCG TGTCCCCCAA AAAGTCCACT GTGCTTCAGC AGCAGTATAA CCGGGTGGGG
    AAGGTGGAGC ATGGCTCCGT GGCCCTGCCC GCCATCATGC GCTCCGGAGC CTCGGGTCCC
    GAGAATTTCC AGGTGGGCAG CATGCCACCT GCCCAGCAGC AGATCACCAG TGGCCAGATG
    CACCGAGGAC ACATGCCTCC TTTGACGTCA GCCCAGCAGG CGCTCACTGG GACCATCAAC
    TCCAGCATGC AGGCTGTGCA AGCTGCCCAG GCCACACTGG ATGACTTTGA CACTCTGCCC
    CCTCTGGGCC AGGACGCTGC CTCAAAGGCC TGGCGTAAGA ACAAGATGGA TGAATCCAAG
    CATGAAATCC ACTCCCAGGT AGACGCCATC ACCGCTGGTA CCGCCTCTGT GGTGAACCTC
    ACAGCAGGGG ACCCTGCTGA GACAGACTAC ACGGCAGTGG GCTGTGCGGT CACCACCATC
    TCCTCCAACC TGACGGAGAT GTCCCGTGGC GTGAAGCTGC TTGCTGCCCT GCTGGAGGAT
    GAAGGCGGCA GCGGCCGGCC CCTGCTGCAG GCGGCCAAGG GCCTGGCGGG GGCGGTGTCA
    GAGCTGCTGC GCAGTGCCCA GCCAGCCAGT GCCGAGCCCC GTCAGAACCT GCTGCAAGCA
    GCTGGGAACG TGGGCCAGGC CAGTGGGGAG CTGTTGCAAC AAATTGGGGA AAGTGATACT
    GACCCCCACT TCCAGGACGT GCTCATGCAG CTAGCCAAGG CCGTGGCAAG CGCTGCAGCT
    GCCCTGGTCC TCAAGGCCAA GAGTGTGGCC CAGCGAACAG AGGACTCAGG GCTTCAGACC
    CAGGTCATCG CTGCGGCAAC ACAGTGTGCC CTGTCCACCT CCCAGCTGGT GGCCTGTACC
    AAGGTGGTGG CTCCTACTAT CAGCTCCCCA GTCTGCCAAG AACAGCTGGT AGAGGCTGGG
    CGACTAGTGG CCAAGGCTGT GGAGGGCTGT GTGTCCGCTT CCCAGGCCGC CACAGAGGAT
    GCCCAGCTGT TACGGGGGGT AGGTGCTGCG GCCACAGCTG TCACCCAGGC CCTGAATGAG
    CTGCTGCAGC ACGTGAGGGC CCATGCCACA GGGGCTGGGC CTGCTGGCCG CTACGACCAG
    GCCACTGACA CCATCCTCAC TGTCACCGAG AACATCTTCA GCTCCATGGG TGATGCTGGT
    GAGATGGTGC GACAGGCCCG CATCCTCGCC CAAGCCACCT CTGACCTGGT GAACGCCATC
    AAGGCTGATG CCGAAGGGGA GAGTGATCTG GAGAATTCCC GCAAGCTGTT AAGTGCCGCC
    AAGATCCTGG CCGATGCCAC AGCCAAGATG GTGGAGGCTG CCAAGGGAGC AGCCGCCAAT
    CCTGACAGTG AGGAGCAACA GCAGCGGCTG CGGGAGGCAG CAGAGGGTCT CCGCATGGCA
    ACCAATGCCG CTGCACAGAA CGCCATCAAG AAAAAGCTGG TGCAGCGCCT TGAGCATGCA
    GCCAAACAGG CTGCAGCCTC GGCTACACAG ACTATCGCAG CAGCCCAACA TGCAACCTCC
    ACTCCCAAGG CCTCTGCTGG CCCCCAGCCC CTGCTGGTGC AGAGCTGCAA GGCTGTGGCA
    GAGCAGATTC CACTGCTGGT GCAGGGCGTC CGAGGGAGCC AAGCGCAGCC TGACAGCCCC
    AGTGCTCAGC TAGCCCTCAT CGCTGCCAGC CAAAGCTTCC TGCAGCCAGG TGGGAAGATG
    GTGGCAGCCG CAAAGGCCTC TGTGCCAACA ATTCAGGACC AAGCATCGGC CATGCAGCTG
    AGTCAGTGTG CTAAAAACCT TGGCACCGCG TTGGCTGAAC TCCGTACTGC TGCCCAGAAG
    GCTCAGGAAG CGTGTGGGCC TTTGGAAATG GATTCTGCTC TGAGTGTGGT GCAGAATCTG
    GAGAGAGACC TGCAGGAAGT GAAGGCAGCT GCTCGAGACG GCAAGCTGAA GCCCTTGCCT
    GGGGAGACGA TGGAGAAATG TGCCCAGGAC CTGGGCAACA GCACCAAAGC CGTGAGCTCC
    GCTATTGCCC AGCTGCTGGG GGAGGTCGCC CAGGGCAATG AGAATTACGC AGGCATTGCA
    GCTCGGGATG TGGCAGGTGG GCTGCGGTCA CTGGCCCAAG CTGCTAGGGG CGTAGCTGCC
    CTGACATCAG ATCCTGCAGT GCAAGCCATT GTGCTCGACA CAGCCAGCGA TGTCCTGGAC
    AAGGCCAGCA GCCTCATCGA GGAGGCAAAG AAAGCAGCCG GCCATCCAGG GGACCCTGAG
    AGCCAGCAGC GGCTTGCCCA GGTGGCTAAA GCTGTGACCC AGGCCCTGAA CCGTTGCGTC
    AGCTGCCTGC CTGGCCAGCG TGATGTGGAC AATGCCCTGA GGGCAGTGGG AGATGCCAGC
    AAGCGACTCC TGAGTGACTC GCTTCCCCCC AGCACTGGGA CCTTTCAGGA GGCTCAGAGC
    CGGCTGAATG AAGCCGCTGC CGGGCTGAAT CAGGCAGCCA CAGAGCTGGT GCAGGCCTCT
    CGGGGAACCC CTCAGGACCT GGCCCGAGCC TCAGGTCGAT TTGGACAAGA CTTCAGCACC
    TTCCTGGAAG CCGGCGTGGA GATGGCAGGC CAGGCTCCGA GCCAGGAGGA CCGTGCCCAG
    GTCGTATCCA ACTTGAAGGG CATCTCCATG TCTTCAAGCA AACTTCTTCT CGCTGCCAAG
    GCCCTGTCCA CAGACCCTGC AGCCCCAACC CTCAAGAGTC AGCTGGCTGC AGCTGCCCGG
    GCAGTGACCG ACAGCATCAA CCAGCTCATC AGCATGTGCA CCCAGCAGGC GCCAGGCCAG
    AAGGAGTGTG ATAATGCCCT GCGGGAATTG GAGACGGTCC GGGAACTCCT AGAGAACCCA
    GTCCAGCCCA TCAATGACAT GTCCTACTTT GGCTGTCTGG ACAGCGTCAT GGAAAACTCA
    AAGGTGCTGG GTGAGGCCAT GACCGGCATC TCCCAAAATG CCAAGAACGG AAACTTGCCG
    GAGTTTGGGG AGGCTGTCGC CACAGCCTCC AAGGCCCTCT GCGGCTTCAC CGAGGCAGCT
    GCACAGGCTG CATATCTGGT TGGCGTGTCT GACCCCAACA GCCAAGCTGG ACAACAAGGG
    CTGGTGGAGC CCACACAGTT TGCCCGAGCA AACCAGGCAA TTCAGATGGC CTGTCAGAGT
    TTGGGGGAGC CTGGCTGCAC CCAGGCCCAG GTGCTCTCTG CAGCCACCAT TGTGGCCAAA
    CACACGTCTG CGCTGTGTAA CAGCTGCCGC CTGGCTTCTG CCCGAACCGC CAATCCCACT
    GCCAAGCGCC AGTTCGTACA GTCAGCCAAG GAGGTGGCCA ACAGCACGGC CAACCTTGTC
    AAGACCATCA AGGCGCTGGA TGGGGCCTTC ACAGAGGAGA ACCGTGCCCA GTGCCGAGCA
    GCAACGGCCC CTTTGCTGGA GGCTGTGGAC AATCTGAGCG CCTTTGCGTC CAACCCTGAG
    TTCTCCAGTG TTCCTGCCCA GATCAGCCCT GAGGGCCGGG CTGCCATGGA GCCCATTGTG
    ATCTCTGCTA AGACAATGTT GGAGAGTGCT GGGGGCCTTA TTCAGACAGC CCGAGCCCTG
    GCGGTCAATC CGCGGGACCC ACCACGCTGG TCCGTGCTGG CTGGCCACTC CCGTACTGTC
    TCAGACTCCA TCAAGAAGCT GATCACAAGC ATGAGGGACA AGGCCCCAGG GCAGTTGGAG
    TGTGAGACAG CCATCGCAGC TCTGAACAGC TGTCTGCGGG ACCTTGACCA GGCTTCTCTT
    GCTGCAGTCA GCCAGCAGCT CGCTCCCCGT GAGGGGATCT CTCAGGAGGC CTTGCACACC
    CAGATGCTCA CTGCGGTGCA GGAAATCTCC CATCTTATTG AGCCGCTGGC CAGCGCTGCC
    CGGGCTGAAG CCTCCCAGCT GGGACACAAG GTGTCCCAGA TGGCCCAGTA CTTTGAGCCG
    CTCACTCTGG CTGCCGTGGG TGCTGCCTCC AAAACCCTGA GCCACCCACA GCAGATGGCG
    CTCCTGGACC AGACCAAAAC CTTGGCCGAG TCTGCCCTGC AGCTGCTATA CACAGCCAAG
    GAAGCTGGCG GTAACCCCAA GCAAGCAGCT CACACCCAGG AAGCCCTGGA GGAGGCTGTG
    CAGATGATGA CAGAGGCCGT AGAGGACCTG ACAACCACCC TCAACGAGGC AGCCAGTGCT
    GCCGGGGTCG TCGGTGGCAT GGTGGACTCC ATCACCCAGG CCATCAACCA GCTAGATGAA
    GGACCAATGG GTGAACCAGA GGGTTCCTTC GTGGATTACC AGACCACCAT GGTGCGGACA
    GCCAAGGCCA TTGCTGTCAC CGTTCAGGAG ATGGTAACCA AGTCAAACAC CAGCCCTGAG
    GAGCTGGGCC CTCTCGCTAA CCAGCTGACT AGTGACTATG GGCGTCTTGC CTCCCAGGCC
    AAGCCTGCAG CTGTGGCTGC TGAAAATGAA GAGATAGGCG CCCACATCAA GCACCGGGTT
    CAGGAGCTGG GGCACGGCTG CGCAGCTCTG GTCACCAAGG CAGGCGCCCT GCAGTGTAGC
    CCTAGTGATG CCTACACCAA GAAGGAGCTC ATAGAGTGTG CCCGGAGAGT CTCTGAGAAG
    GTCTCCCACG TGCTGGCTGC GCTCCAGGCT GGGAATCGTG GCACCCAGGC CTGCATCACA
    GCCGCCAGCG CTGTGTCCGG CATCATCGCC GACCTGGACA CCACCATCAT GTTCGCCACT
    GCCGGCACAC TCAACCGTGA GGGTGCCGAA ACCTTTGCTG ACCACCGAGA GGGCATTCTG
    AAGACTGCCA AGGTGCTGGT GGAAGACACC AAGGTCCTGG TGCAGAATGC AGCTGGGAGC
    CAGGAGAAGC TGGCACAGGC AGCCCAGTCC TCCGTGGCCA CCATCACCCG CCTCGCCGAT
    GTGGTCAAGC TGGGTGCAGC CAGCCTGGGA GCTGAGGACC CAGAGACCCA GGTGGTGCTG
    ATCAATGCAG TGAAAGATGT GGCCAAGGCC CTGGGAGACC TCATCAGTGC CACAAAGGCT
    GCGGCTGGCA AAGTCGGCGA CGACCCTGCT GTGTGGCAGC TCAAGAACTC TGCCAAGGTG
    ATGGTAACCA ATGTGACATC ACTGCTCAAG ACCGTGAAAG CGGTAGAGGA TGAGGCCACC
    AAAGGCACGC GCGCCCTGGA GGCCACAACG GAGCATATTC GGCAGGAGCT GGCGGTCTTC
    TGCTCCCCAG AGCCACCTGC CAAGACCTCG ACCCCTGAAG ATTTCATCCG CATGACCAAG
    GGTATCACCA TGGCAACCGC CAAGGCCGTT GCCGCTGGGA ACTCCTGTCG CCAGGAGGAT
    GTCATTGCCA CAGCCAATCT GAGTCGCCGT GCTATTGCAG ATATGCTCCG GGCTTGCAAG
    GAAGCTGCCT ACCACCCAGA AGTGGCTCCA GACGTGCGGC TCCGAGCCCT ACACTTTGGC
    CGGGAATGTG CCAATGGCTA CCTGGAACTG CTGGACCACG TGCTGCTGAC CCTGCAGAAG
    CCAAGCCCAG AATTGAAGCA GCAATTGACG GTGCACTCAA AGCGTGTGGC TGGATCAGTC
    ACTGAGCTCA TCCAGGCTGC TGAGGCCATG AAGGGAACAG AATGGGTGGA CCCAGAGGAC
    CCCACAGTCA TTGCTGAGAA TGAACTCCTG GGAGCCGCGG CTGCCATTGA GGCTGCGGCC
    AAGAAGCTGG AGCAGCTGAA GCCCCGGGCC AAACCTAAGG AGGCGGACGA GTCCTTGAAC
    TTTGAGGAGC AGATACTGGA AGCTGCCAAG TCCATCGCAG CAGCCACCAG CGCCCTGGTG
    AAGGCTGCCT CAGCTGCACA GAGGGAACTG GTGGCCCAGG GGAAGGTGGG CGCCATTCCA
    GCCAACGCAC TGGATGATGG ACAGTGGTCC CAGGGCCTCA TCTCTGCTGC CCGGATGGTG
    GCTGCGGCCA CCAACAACCT GTGTGAGGCA GCCAATGCAG CTGTCCAAGG CCACGCCAGC
    CAGGAGAAGC TCATCTCATC AGCCAAGCAG GTCGCCGCCT CCACAGCCCA GCTCCTCGTA
    GCCTGCAAGG TCAAGGCCGA CCAGGACTCT GAGGCAATGA AGCGGCTTCA GGCTGCCGGC
    AACGCAGTGA AGCGAGCCTC AGACAACCTG GTGAAGGCAG CCCAGAAGGC TGCAGCCTTT
    GAAGAACAGG AGAATGAGAC CGTGGTGGTG AAGGAGAAGA TGGTCGGGGG CATTGCCCAG
    ATCATTGCTG CCCAGGAAGA GATGCTTCGG AAGGAACGAG AGCTGGAGGA GGCACGGAAG
    AAGCTGGCGC AGATCCGGCA GCAGCAGTAC AAATTCTTAC CTTCAGAGCT TCGAGACGAG
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851763.1
    CDS455..8080
    Translation

    Target ORF information:

    RefSeq Version XM_024995995.1
    Organism Bos taurus(cattle)
    Definition Bos taurus talin 1 (TLN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995995.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTTGCAC TTTCGCTGAA GATCAGCATT GGGAATGTGG TGAAGACGAT GCAATTTGAG 
    CCGTCTACCA TGGTGTATGA CGCCTGTCGG ATCATCCGTG AGCGGATCCC AGAGGCCCTA
    TCTGGCCCTC CCAGCGACTT TGGGCTGTTT CTGTCAGATG ATGACCCCAA AAAGGGTATA
    TGGCTGGAGG CAGGGAAGGC TTTGGATTAT TACATGCTCC GAAACGGGGA CACCATGGAG
    TATAGGAAGA AACAGAGGCC CTTGAAGATC CGCATGCTGG ACGGCACCGT GAAGACTGTC
    ATGGTGGACG ACTCGAAGAC TGTCACGGAC ATGCTCATGA CCATCTGTGC CCGCATTGGC
    ATCACCAACC ATGATGAATA TTCACTGGTT CGAGAGATTA TGGAAGAGAA GAAGGAGGAG
    GTGACAGGGA CCTTACGAAA GGACAAGACA CTGCTGCGAG ACGAAAAGAA GATGGAGAAA
    CTGAAGCAGA AGTTGCACAC AGATGACGAG TTGAACTGGC TGGACCATGG CCGGACCCTG
    AGGGAGCAGG GAGTGGAGGA GCACGAAACG CTTCTGCTCC GGAGGAAGTT CTTCTACTCA
    GATCAGAACG TGGACTCCCG GGACCCCGTC CAGCTGAACC TTCTCTACGT GCAGGCACGA
    GATGACATCC TGAATGGCTC CCATCCTGTC TCCTTCGACA AGGCCTGTGA GTTTGCTGGC
    TTCCAATGCC AGATCCAGTT TGGGCCCCAC AATGAGCAGA AGCACAAGGC CGGCTTCCTC
    GACCTGAAGG ACTTCCTGCC CAAGGAGTAT GTGAAGCAGA AGGGAGAGCG TAAGATCTTC
    CAGGCACACA AGAACTGTGG ACAGATGAGT GAGATTGAGG CCAAGGTACG CTACGTGAAG
    CTAGCCCGTT CCCTCAAGAC GTACGGTGTC TCCTTCTTCC TGGTCAAGGA AAAGATGAAA
    GGAAAGAACA AGTTGGTGCC CAGGCTTCTG GGCATCACTA AGGAGTGTGT GATGCGAGTG
    GACGAGAAGA CCAAGGAGGT GATCCAGGAA TGGAACCTCA CCAACATAAA ACGCTGGGCT
    GCGTCTCCCA AGAGCTTCAC CCTGGATTTC GGAGACTACC AAGATGGCTA CTACTCGGTG
    CAGACGACCG AGGGGGAGCA GATCGCTCAG CTCATCGCTG GCTACATCGA CATCATCCTT
    AAGAAGAAAA AAAGCAAGGA TCATTTTGGG CTGGAGGGAG ACGAGGAGTC TACTATGCTG
    GAGGACTCCG TGTCCCCCAA AAAGTCCACT GTGCTTCAGC AGCAGTATAA CCGGGTGGGG
    AAGGTGGAGC ATGGCTCCGT GGCCCTGCCC GCCATCATGC GCTCCGGAGC CTCGGGTCCC
    GAGAATTTCC AGGTGGGCAG CATGCCACCT GCCCAGCAGC AGATCACCAG TGGCCAGATG
    CACCGAGGAC ACATGCCTCC TTTGACGTCA GCCCAGCAGG CGCTCACTGG GACCATCAAC
    TCCAGCATGC AGGCTGTGCA AGCTGCCCAG GCCACACTGG ATGACTTTGA CACTCTGCCC
    CCTCTGGGCC AGGACGCTGC CTCAAAGGCC TGGCGTAAGA ACAAGATGGA TGAATCCAAG
    CATGAAATCC ACTCCCAGGT AGACGCCATC ACCGCTGGTA CCGCCTCTGT GGTGAACCTC
    ACAGCAGGGG ACCCTGCTGA GACAGACTAC ACGGCAGTGG GCTGTGCGGT CACCACCATC
    TCCTCCAACC TGACGGAGAT GTCCCGTGGC GTGAAGCTGC TTGCTGCCCT GCTGGAGGAT
    GAAGGCGGCA GCGGCCGGCC CCTGCTGCAG GCGGCCAAGG GCCTGGCGGG GGCGGTGTCA
    GAGCTGCTGC GCAGTGCCCA GCCAGCCAGT GCCGAGCCCC GTCAGAACCT GCTGCAAGCA
    GCTGGGAACG TGGGCCAGGC CAGTGGGGAG CTGTTGCAAC AAATTGGGGA AAGTGATACT
    GACCCCCACT TCCAGGACGT GCTCATGCAG CTAGCCAAGG CCGTGGCAAG CGCTGCAGCT
    GCCCTGGTCC TCAAGGCCAA GAGTGTGGCC CAGCGAACAG AGGACTCAGG GCTTCAGACC
    CAGGTCATCG CTGCGGCAAC ACAGTGTGCC CTGTCCACCT CCCAGCTGGT GGCCTGTACC
    AAGGTGGTGG CTCCTACTAT CAGCTCCCCA GTCTGCCAAG AACAGCTGGT AGAGGCTGGG
    CGACTAGTGG CCAAGGCTGT GGAGGGCTGT GTGTCCGCTT CCCAGGCCGC CACAGAGGAT
    GCCCAGCTGT TACGGGGGGT AGGTGCTGCG GCCACAGCTG TCACCCAGGC CCTGAATGAG
    CTGCTGCAGC ACGTGAGGGC CCATGCCACA GGGGCTGGGC CTGCTGGCCG CTACGACCAG
    GCCACTGACA CCATCCTCAC TGTCACCGAG AACATCTTCA GCTCCATGGG TGATGCTGGT
    GAGATGGTGC GACAGGCCCG CATCCTCGCC CAAGCCACCT CTGACCTGGT GAACGCCATC
    AAGGCTGATG CCGAAGGGGA GAGTGATCTG GAGAATTCCC GCAAGCTGTT AAGTGCCGCC
    AAGATCCTGG CCGATGCCAC AGCCAAGATG GTGGAGGCTG CCAAGGGAGC AGCCGCCAAT
    CCTGACAGTG AGGAGCAACA GCAGCGGCTG CGGGAGGCAG CAGAGGGTCT CCGCATGGCA
    ACCAATGCCG CTGCACAGAA CGCCATCAAG AAAAAGCTGG TGCAGCGCCT TGAGCATGCA
    GCCAAACAGG CTGCAGCCTC GGCTACACAG ACTATCGCAG CAGCCCAACA TGCAACCTCC
    ACTCCCAAGG CCTCTGCTGG CCCCCAGCCC CTGCTGGTGC AGAGCTGCAA GGCTGTGGCA
    GAGCAGATTC CACTGCTGGT GCAGGGCGTC CGAGGGAGCC AAGCGCAGCC TGACAGCCCC
    AGTGCTCAGC TAGCCCTCAT CGCTGCCAGC CAAAGCTTCC TGCAGCCAGG TGGGAAGATG
    GTGGCAGCCG CAAAGGCCTC TGTGCCAACA ATTCAGGACC AAGCATCGGC CATGCAGCTG
    AGTCAGTGTG CTAAAAACCT TGGCACCGCG TTGGCTGAAC TCCGTACTGC TGCCCAGAAG
    GCTCAGGAAG CGTGTGGGCC TTTGGAAATG GATTCTGCTC TGAGTGTGGT GCAGAATCTG
    GAGAGAGACC TGCAGGAAGT GAAGGCAGCT GCTCGAGACG GCAAGCTGAA GCCCTTGCCT
    GGGGAGACGA TGGAGAAATG TGCCCAGGAC CTGGGCAACA GCACCAAAGC CGTGAGCTCC
    GCTATTGCCC AGCTGCTGGG GGAGGTCGCC CAGGGCAATG AGAATTACGC AGGCATTGCA
    GCTCGGGATG TGGCAGGTGG GCTGCGGTCA CTGGCCCAAG CTGCTAGGGG CGTAGCTGCC
    CTGACATCAG ATCCTGCAGT GCAAGCCATT GTGCTCGACA CAGCCAGCGA TGTCCTGGAC
    AAGGCCAGCA GCCTCATCGA GGAGGCAAAG AAAGCAGCCG GCCATCCAGG GGACCCTGAG
    AGCCAGCAGC GGCTTGCCCA GGTGGCTAAA GCTGTGACCC AGGCCCTGAA CCGTTGCGTC
    AGCTGCCTGC CTGGCCAGCG TGATGTGGAC AATGCCCTGA GGGCAGTGGG AGATGCCAGC
    AAGCGACTCC TGAGTGACTC GCTTCCCCCC AGCACTGGGA CCTTTCAGGA GGCTCAGAGC
    CGGCTGAATG AAGCCGCTGC CGGGCTGAAT CAGGCAGCCA CAGAGCTGGT GCAGGCCTCT
    CGGGGAACCC CTCAGGACCT GGCCCGAGCC TCAGGTCGAT TTGGACAAGA CTTCAGCACC
    TTCCTGGAAG CCGGCGTGGA GATGGCAGGC CAGGCTCCGA GCCAGGAGGA CCGTGCCCAG
    GTCGTATCCA ACTTGAAGGG CATCTCCATG TCTTCAAGCA AACTTCTTCT CGCTGCCAAG
    GCCCTGTCCA CAGACCCTGC AGCCCCAACC CTCAAGAGTC AGCTGGCTGC AGCTGCCCGG
    GCAGTGACCG ACAGCATCAA CCAGCTCATC AGCATGTGCA CCCAGCAGGC GCCAGGCCAG
    AAGGAGTGTG ATAATGCCCT GCGGGAATTG GAGACGGTCC GGGAACTCCT AGAGAACCCA
    GTCCAGCCCA TCAATGACAT GTCCTACTTT GGCTGTCTGG ACAGCGTCAT GGAAAACTCA
    AAGGTGCTGG GTGAGGCCAT GACCGGCATC TCCCAAAATG CCAAGAACGG AAACTTGCCG
    GAGTTTGGGG AGGCTGTCGC CACAGCCTCC AAGGCCCTCT GCGGCTTCAC CGAGGCAGCT
    GCACAGGCTG CATATCTGGT TGGCGTGTCT GACCCCAACA GCCAAGCTGG ACAACAAGGG
    CTGGTGGAGC CCACACAGTT TGCCCGAGCA AACCAGGCAA TTCAGATGGC CTGTCAGAGT
    TTGGGGGAGC CTGGCTGCAC CCAGGCCCAG GTGCTCTCTG CAGCCACCAT TGTGGCCAAA
    CACACGTCTG CGCTGTGTAA CAGCTGCCGC CTGGCTTCTG CCCGAACCGC CAATCCCACT
    GCCAAGCGCC AGTTCGTACA GTCAGCCAAG GAGGTGGCCA ACAGCACGGC CAACCTTGTC
    AAGACCATCA AGGCGCTGGA TGGGGCCTTC ACAGAGGAGA ACCGTGCCCA GTGCCGAGCA
    GCAACGGCCC CTTTGCTGGA GGCTGTGGAC AATCTGAGCG CCTTTGCGTC CAACCCTGAG
    TTCTCCAGTG TTCCTGCCCA GATCAGCCCT GAGGGCCGGG CTGCCATGGA GCCCATTGTG
    ATCTCTGCTA AGACAATGTT GGAGAGTGCT GGGGGCCTTA TTCAGACAGC CCGAGCCCTG
    GCGGTCAATC CGCGGGACCC ACCACGCTGG TCCGTGCTGG CTGGCCACTC CCGTACTGTC
    TCAGACTCCA TCAAGAAGCT GATCACAAGC ATGAGGGACA AGGCCCCAGG GCAGTTGGAG
    TGTGAGACAG CCATCGCAGC TCTGAACAGC TGTCTGCGGG ACCTTGACCA GGCTTCTCTT
    GCTGCAGTCA GCCAGCAGCT CGCTCCCCGT GAGGGGATCT CTCAGGAGGC CTTGCACACC
    CAGATGCTCA CTGCGGTGCA GGAAATCTCC CATCTTATTG AGCCGCTGGC CAGCGCTGCC
    CGGGCTGAAG CCTCCCAGCT GGGACACAAG GTGTCCCAGA TGGCCCAGTA CTTTGAGCCG
    CTCACTCTGG CTGCCGTGGG TGCTGCCTCC AAAACCCTGA GCCACCCACA GCAGATGGCG
    CTCCTGGACC AGACCAAAAC CTTGGCCGAG TCTGCCCTGC AGCTGCTATA CACAGCCAAG
    GAAGCTGGCG GTAACCCCAA GCAAGCAGCT CACACCCAGG AAGCCCTGGA GGAGGCTGTG
    CAGATGATGA CAGAGGCCGT AGAGGACCTG ACAACCACCC TCAACGAGGC AGCCAGTGCT
    GCCGGGGTCG TCGGTGGCAT GGTGGACTCC ATCACCCAGG CCATCAACCA GCTAGATGAA
    GGACCAATGG GTGAACCAGA GGGTTCCTTC GTGGATTACC AGACCACCAT GGTGCGGACA
    GCCAAGGCCA TTGCTGTCAC CGTTCAGGAG ATGGTAACCA AGTCAAACAC CAGCCCTGAG
    GAGCTGGGCC CTCTCGCTAA CCAGCTGACT AGTGACTATG GGCGTCTTGC CTCCCAGGCC
    AAGCCTGCAG CTGTGGCTGC TGAAAATGAA GAGATAGGCG CCCACATCAA GCACCGGGTT
    CAGGAGCTGG GGCACGGCTG CGCAGCTCTG GTCACCAAGG CAGGCGCCCT GCAGTGTAGC
    CCTAGTGATG CCTACACCAA GAAGGAGCTC ATAGAGTGTG CCCGGAGAGT CTCTGAGAAG
    GTCTCCCACG TGCTGGCTGC GCTCCAGGCT GGGAATCGTG GCACCCAGGC CTGCATCACA
    GCCGCCAGCG CTGTGTCCGG CATCATCGCC GACCTGGACA CCACCATCAT GTTCGCCACT
    GCCGGCACAC TCAACCGTGA GGGTGCCGAA ACCTTTGCTG ACCACCGAGA GGGCATTCTG
    AAGACTGCCA AGGTGCTGGT GGAAGACACC AAGGTCCTGG TGCAGAATGC AGCTGGGAGC
    CAGGAGAAGC TGGCACAGGC AGCCCAGTCC TCCGTGGCCA CCATCACCCG CCTCGCCGAT
    GTGGTCAAGC TGGGTGCAGC CAGCCTGGGA GCTGAGGACC CAGAGACCCA GGTGGTGCTG
    ATCAATGCAG TGAAAGATGT GGCCAAGGCC CTGGGAGACC TCATCAGTGC CACAAAGGCT
    GCGGCTGGCA AAGTCGGCGA CGACCCTGCT GTGTGGCAGC TCAAGAACTC TGCCAAGGTG
    ATGGTAACCA ATGTGACATC ACTGCTCAAG ACCGTGAAAG CGGTAGAGGA TGAGGCCACC
    AAAGGCACGC GCGCCCTGGA GGCCACAACG GAGCATATTC GGCAGGAGCT GGCGGTCTTC
    TGCTCCCCAG AGCCACCTGC CAAGACCTCG ACCCCTGAAG ATTTCATCCG CATGACCAAG
    GGTATCACCA TGGCAACCGC CAAGGCCGTT GCCGCTGGGA ACTCCTGTCG CCAGGAGGAT
    GTCATTGCCA CAGCCAATCT GAGTCGCCGT GCTATTGCAG ATATGCTCCG GGCTTGCAAG
    GAAGCTGCCT ACCACCCAGA AGTGGCTCCA GACGTGCGGC TCCGAGCCCT ACACTTTGGC
    CGGGAATGTG CCAATGGCTA CCTGGAACTG CTGGACCACG TGCTGCTGAC CCTGCAGAAG
    CCAAGCCCAG AATTGAAGCA GCAATTGACG GTGCACTCAA AGCGTGTGGC TGGATCAGTC
    ACTGAGCTCA TCCAGGCTGC TGAGGCCATG AAGGGAACAG AATGGGTGGA CCCAGAGGAC
    CCCACAGTCA TTGCTGAGAA TGAACTCCTG GGAGCCGCGG CTGCCATTGA GGCTGCGGCC
    AAGAAGCTGG AGCAGCTGAA GCCCCGGGCC AAACCTAAGG AGGCGGACGA GTCCTTGAAC
    TTTGAGGAGC AGATACTGGA AGCTGCCAAG TCCATCGCAG CAGCCACCAG CGCCCTGGTG
    AAGGCTGCCT CAGCTGCACA GAGGGAACTG GTGGCCCAGG GGAAGGTGGG CGCCATTCCA
    GCCAACGCAC TGGATGATGG ACAGTGGTCC CAGGGCCTCA TCTCTGCTGC CCGGATGGTG
    GCTGCGGCCA CCAACAACCT GTGTGAGGCA GCCAATGCAG CTGTCCAAGG CCACGCCAGC
    CAGGAGAAGC TCATCTCATC AGCCAAGCAG GTCGCCGCCT CCACAGCCCA GCTCCTCGTA
    GCCTGCAAGG TCAAGGCCGA CCAGGACTCT GAGGCAATGA AGCGGCTTCA GGCTGCCGGC
    AACGCAGTGA AGCGAGCCTC AGACAACCTG GTGAAGGCAG CCCAGAAGGC TGCAGCCTTT
    GAAGAACAGG AGAATGAGAC CGTGGTGGTG AAGGAGAAGA TGGTCGGGGG CATTGCCCAG
    ATCATTGCTG CCCAGGAAGA GATGCTTCGG AAGGAACGAG AGCTGGAGGA GGCACGGAAG
    AAGCTGGCGC AGATCCGGCA GCAGCAGTAC AAATTCTTAC CTTCAGAGCT TCGAGACGAG
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa41129
    Clone ID Related Accession (Same CDS sequence) NM_001205428.1
    Accession Version NM_001205428.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product talin-1
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03005013.1 and AAFC03026449.1. On Apr 20, 2011 this sequence version replaced XM_001250861.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTTGCAC TTTCGCTGAA GATCAGCATT GGGAATGTGG TGAAGACGAT GCAATTTGAG 
    CCGTCTACCA TGGTGTATGA CGCCTGTCGG ATCATCCGTG AGCGGATCCC AGAGGCCCTA
    TCTGGCCCTC CCAGCGACTT TGGGCTGTTT CTGTCAGATG ATGACCCCAA AAAGGGTATA
    TGGCTGGAGG CAGGGAAGGC TTTGGATTAT TACATGCTCC GAAACGGGGA CACCATGGAG
    TATAGGAAGA AACAGAGGCC CTTGAAGATC CGCATGCTGG ACGGCACCGT GAAGACTGTC
    ATGGTGGACG ACTCGAAGAC TGTCACGGAC ATGCTCATGA CCATCTGTGC CCGCATTGGC
    ATCACCAACC ATGATGAATA TTCACTGGTT CGAGAGATTA TGGAAGAGAA GAAGGAGGAG
    GTGACAGGGA CCTTACGAAA GGACAAGACA CTGCTGCGAG ACGAAAAGAA GATGGAGAAA
    CTGAAGCAGA AGTTGCACAC AGATGACGAG TTGAACTGGC TGGACCATGG CCGGACCCTG
    AGGGAGCAGG GAGTGGAGGA GCACGAAACG CTTCTGCTCC GGAGGAAGTT CTTCTACTCA
    GATCAGAACG TGGACTCCCG GGACCCCGTC CAGCTGAACC TTCTCTACGT GCAGGCACGA
    GATGACATCC TGAATGGCTC CCATCCTGTC TCCTTCGACA AGGCCTGTGA GTTTGCTGGC
    TTCCAATGCC AGATCCAGTT TGGGCCCCAC AATGAGCAGA AGCACAAGGC CGGCTTCCTC
    GACCTGAAGG ACTTCCTGCC CAAGGAGTAT GTGAAGCAGA AGGGAGAGCG TAAGATCTTC
    CAGGCACACA AGAACTGTGG ACAGATGAGT GAGATTGAGG CCAAGGTACG CTACGTGAAG
    CTAGCCCGTT CCCTCAAGAC GTACGGTGTC TCCTTCTTCC TGGTCAAGGA AAAGATGAAA
    GGAAAGAACA AGTTGGTGCC CAGGCTTCTG GGCATCACTA AGGAGTGTGT GATGCGAGTG
    GACGAGAAGA CCAAGGAGGT GATCCAGGAA TGGAACCTCA CCAACATAAA ACGCTGGGCT
    GCGTCTCCCA AGAGCTTCAC CCTGGATTTT GGAGACTACC AAGATGGCTA CTACTCGGTG
    CAGACGACCG AGGGGGAGCA GATCGCTCAG CTCATCGCTG GCTACATCGA CATCATCCTT
    AAGAAGAAAA AAAGCAAGGA TCATTTTGGG CTGGAGGGAG ACGAGGAGTC TACTATGCTG
    GAGGACTCCG TGTCCCCCAA AAAGTCCACT GTGCTTCAGC AGCAGTATAA CCGGGTGGGG
    AAGGTGGAGC ATGGCTCCGT GGCCCTGCCC GCCATCATGC GCTCCGGAGC CTCGGGTCCC
    GAGAATTTCC AGGTGGGCAG CATGCCACCT GCCCAGCAGC AGATCACCAG TGGCCAGATG
    CACCGAGGAC ACATGCCTCC TTTGACGTCA GCCCAGCAGG CGCTCACTGG GACCATCAAC
    TCCAGCATGC AGGCTGTGCA AGCTGCCCAG GCCACACTGG ATGACTTTGA CACTCTGCCC
    CCTCTGGGCC AGGACGCTGC CTCAAAGGCC TGGCGTAAGA ACAAGATGGA TGAATCCAAG
    CATGAAATCC ACTCCCAGGT AGACGCCATC ACCGCTGGTA CCGCCTCTGT GGTGAACCTC
    ACAGCAGGGG ACCCTGCTGA GACAGACTAC ACGGCAGTGG GCTGTGCGGT CACCACCATC
    TCCTCCAACC TGACGGAGAT GTCCCGTGGC GTGAAGCTGC TTGCTGCCCT GCTGGAGGAT
    GAAGGCGGCA GCGGCCGGCC CCTGCTGCAG GCGGCCAAGG GCCTGGCGGG GGCGGTGTCA
    GAGCTGCTGC GCAGTGCCCA GCCAGCCAGT GCCGAGCCCC GTCAGAACCT GCTGCAAGCA
    GCTGGGAACG TGGGCCAGGC CAGTGGGGAG CTGTTGCAAC AAATTGGGGA AAGTGATACT
    GACCCCCACT TCCAGGACGT GCTCATGCAG CTAGCCAAGG CCGTGGCAAG CGCTGCAGCT
    GCCCTGGTCC TCAAGGCCAA GAGTGTGGCC CAGCGAACAG AGGACTCAGG GCTTCAGACC
    CAGGTCATCG CTGCGGCAAC ACAGTGTGCC CTGTCCACCT CCCAGCTGGT GGCCTGTACC
    AAGGTGGTGG CTCCTACTAT CAGCTCCCCA GTCTGCCAAG AACAGCTGGT AGAGGCTGGG
    CGACTAGTGG CCAAGGCTGT GGAGGGCTGT GTGTCCGCTT CCCAGGCCGC CACAGAGGAT
    GCCCAGCTGT TACGGGGGGT AGGTGCTGCG GCCACAGCTG TCACCCAGGC CCTGAATGAG
    CTGCTGCAGC ACGTGAGGGC CCATGCCACA GGGGCTGGGC CTGCTGGCCG CTACGACCAG
    GCCACTGACA CCATCCTCAC TGTCACCGAG AACATCTTCA GCTCCATGGG TGATGCTGGT
    GAGATGGTGC GACAGGCCCG CATCCTCGCC CAAGCCACCT CTGACCTGGT GAACGCCATC
    AAGGCTGATG CCGAAGGGGA GAGTGATCTG GAGAATTCCC GCAAGCTGTT AAGTGCCGCC
    AAGATCCTGG CCGATGCCAC AGCCAAGATG GTGGAGGCTG CCAAGGGAGC AGCCGCCAAT
    CCTGACAGTG AGGAGCAACA GCAGCGGCTG CGGGAGGCAG CAGAGGGTCT CCGCATGGCA
    ACCAATGCCG CTGCACAGAA CGCCATCAAG AAAAAGCTGG TGCAGCGCCT TGAGCATGCA
    GCCAAACAGG CTGCAGCCTC GGCTACACAG ACTATCGCAG CAGCCCAACA TGCAACCTCC
    ACTCCCAAGG CCTCTGCTGG CCCCCAGCCC CTGCTGGTGC AGAGCTGCAA GGCTGTGGCA
    GAGCAGATTC CACTGCTGGT GCAGGGCGTC CGAGGGAGCC AAGCGCAGCC TGACAGCCCC
    AGTGCTCAGC TAGCCCTCAT CGCTGCCAGC CAAAGCTTCC TGCAGCCAGG TGGGAAGATG
    GTGGCAGCCG CAAAGGCCTC TGTGCCAACA ATTCAGGACC AAGCATCGGC CATGCAGCTG
    AGTCAGTGTG CTAAAAACCT TGGCACCGCG TTGGCTGAAC TCCGTACTGC TGCCCAGAAG
    GCTCAGGAAG CGTGTGGGCC TTTGGAAATG GATTCTGCTC TGAGTGTGGT GCAGAATCTG
    GAGAGAGACC TGCAGGAAGT GAAGGCAGCT GCTCGAGACG GCAAGCTGAA GCCCTTGCCT
    GGGGAGACGA TGGAGAAATG TGCCCAGGAC CTGGGCAACA GCACCAAAGC CGTGAGCTCC
    GCTATTGCCC AGCTGCTGGG GGAGGTCGCC CAGGGCAATG AGAATTACGC AGGCATTGCA
    GCTCGGGATG TGGCAGGTGG GCTGCGGTCA CTGGCCCAAG CTGCTAGGGG CGTAGCTGCC
    CTGACATCAG ATCCTGCAGT GCAAGCCATT GTGCTCGACA CAGCCAGCGA TGTCCTGGAC
    AAGGCCAGCA GCCTCATCGA GGAGGCAAAG AAAGCAGCCG GCCATCCAGG GGACCCTGAG
    AGCCAGCAGC GGCTTGCCCA GGTGGCTAAA GCTGTGACCC AGGCCCTGAA CCGTTGCGTC
    AGCTGCCTGC CTGGCCAGCG TGATGTGGAC AATGCCCTGA GGGCAGTGGG AGATGCCAGC
    AAGCGACTCC TGAGTGACTC GCTTCCCCCC AGCACTGGGA CCTTTCAGGA GGCTCAGAGC
    CGGCTGAATG AAGCCGCTGC CGGGCTGAAT CAGGCAGCCA CAGAGCTGGT GCAGGCCTCT
    CGGGGAACCC CTCAGGACCT GGCCCGAGCC TCAGGTCGAT TTGGACAAGA CTTCAGCACC
    TTCCTGGAAG CCGGCGTGGA GATGGCAGGC CAGGCTCCGA GCCAGGAGGA CCGTGCCCAG
    GTCGTATCCA ACTTGAAGGG CATCTCCATG TCTTCAAGCA AACTTCTTCT CGCTGCCAAG
    GCCCTGTCCA CAGACCCTGC AGCCCCAACC CTCAAGAGTC AGCTGGCTGC AGCTGCCCGG
    GCAGTGACCG ACAGCATCAA CCAGCTCATC AGCATGTGCA CCCAGCAGGC GCCAGGCCAG
    AAGGAGTGTG ATAATGCCCT GCGGGAATTG GAGACGGTCC GGGAACTCCT AGAGAACCCA
    GTCCAGCCCA TCAATGACAT GTCCTACTTT GGCTGTCTGG ACAGCGTCAT GGAAAACTCA
    AAGGTGCTGG GTGAGGCCAT GACCGGCATC TCCCAAAATG CCAAGAACGG AAACTTGCCG
    GAGTTTGGGG AGGCTGTCGC CACAGCCTCC AAGGCCCTCT GCGGCTTCAC CGAGGCAGCT
    GCACAGGCTG CATATCTGGT TGGCGTGTCT GACCCCAACA GCCAAGCTGG ACAACAAGGG
    CTGGTGGAGC CCACACAGTT TGCCCGAGCA AACCAGGCAA TTCAGATGGC CTGTCAGAGT
    TTGGGGGAGC CTGGCTGCAC CCAGGCCCAG GTGCTCTCTG CAGCCACCAT TGTGGCCAAA
    CACACGTCTG CGCTGTGTAA CAGCTGCCGC CTGGCTTCTG CCCGAACCGC CAATCCCACT
    GCCAAGCGCC AGTTCGTACA GTCAGCCAAG GAGGTGGCCA ACAGCACGGC CAACCTTGTC
    AAGACCATCA AGGCGCTGGA TGGGGCCTTC ACAGAGGAGA ACCGTGCCCA GTGCCGAGCA
    GCAACGGCCC CTTTGCTGGA GGCTGTGGAC AATCTGAGCG CCTTTGCGTC CAACCCTGAG
    TTCTCCAGTG TTCCTGCCCA GATCAGCCCT GAGGGCCGGG CTGCCATGGA GCCCATTGTG
    ATCTCTGCTA AGACAATGTT GGAGAGTGCT GGGGGCCTTA TTCAGACAGC CCGAGCCCTG
    GCGGTCAATC CGCGGGACCC ACCACGCTGG TCCGTGCTGG CTGGCCACTC CCGTACTGTC
    TCAGACTCCA TCAAGAAGCT GATCACAAGC ATGAGGGACA AGGCCCCAGG GCAGTTGGAG
    TGTGAGACAG CCATCGCAGC TCTGAACAGC TGTCTGCGGG ACCTTGACCA GGCTTCTCTT
    GCTGCAGTCA GCCAGCAGCT CGCTCCCCGT GAGGGGATCT CTCAGGAGGC CTTGCACACC
    CAGATGCTCA CTGCGGTGCA GGAAATCTCC CATCTTATTG AGCCGCTGGC CAGCGCTGCC
    CGGGCTGAAG CCTCCCAGCT GGGACACAAG GTGTCCCAGA TGGCCCAGTA CTTTGAGCCG
    CTCACTCTGG CTGCCGTGGG TGCTGCCTCC AAAACCCTGA GCCACCCACA GCAGATGGCG
    CTCCTGGACC AGACCAAAAC CTTGGCCGAG TCTGCCCTGC AGCTGCTATA CACAGCCAAG
    GAAGCTGGCG GTAACCCCAA GCAAGCAGCT CACACCCAGG AAGCCCTGGA GGAGGCTGTG
    CAGATGATGA CAGAGGCCGT AGAGGACCTG ACAACCACCC TCAACGAGGC AGCCAGTGCT
    GCCGGGGTCG TCGGTGGCAT GGTGGACTCC ATCACCCAGG CCATCAACCA GCTAGATGAA
    GGACCAATGG GTGAACCAGA GGGTTCCTTC GTGGATTACC AGACCACCAT GGTGCGGACA
    GCCAAGGCCA TTGCTGTCAC CGTTCAGGAG ATGGTAACCA AGTCAAACAC CAGCCCTGAG
    GAGCTGGGCC CTCTCGCTAA CCAGCTGACT AGTGACTATG GGCGTCTTGC CTCCCAGGCC
    AAGCCTGCAG CTGTGGCTGC TGAAAATGAA GAGATAGGCG CCCACATCAA GCACCGGGTT
    CAGGAGCTGG GGCACGGCTG CGCAGCTCTG GTCACCAAGG CAGGCGCCCT GCAGTGTAGC
    CCTAGTGATG CCTACACCAA GAAGGAGCTC ATAGAGTGTG CCCGGAGAGT CTCTGAGAAG
    GTCTCCCACG TGCTGGCTGC GCTCCAGGCT GGGAATCGTG GCACCCAGGC CTGCATCACA
    GCCGCCAGCG CTGTGTCCGG CATCATCGCC GACCTGGACA CCACCATCAT GTTCGCCACT
    GCCGGCACAC TCAACCGTGA GGGTGCCGAA ACCTTTGCTG ACCACCGAGA GGGCATTCTG
    AAGACTGCCA AGGTGCTGGT GGAAGACACC AAGGTCCTGG TGCAGAATGC AGCTGGGAGC
    CAGGAGAAGC TGGCACAGGC AGCCCAGTCC TCCGTGGCCA CCATCACCCG CCTCGCCGAT
    GTGGTCAAGC TGGGTGCAGC CAGCCTGGGA GCTGAGGACC CAGAGACCCA GGTGGTGCTG
    ATCAATGCAG TGAAAGATGT GGCCAAGGCC CTGGGAGACC TCATCAGTGC CACAAAGGCT
    GCGGCTGGCA AAGTCGGCGA CGACCCTGCT GTGTGGCAGC TCAAGAATTC TGCCAAGGTG
    ATGGTAACCA ATGTGACATC ACTGCTCAAG ACCGTGAAAG CGGTAGAGGA TGAGGCCACC
    AAAGGCACGC GCGCCCTGGA GGCCACAACG GAGCATATTC GGCAGGAGCT GGCGGTCTTC
    TGCTCCCCAG AGCCACCTGC CAAGACCTCG ACCCCTGAAG ATTTCATCCG CATGACCAAG
    GGTATCACCA TGGCAACCGC CAAGGCCGTT GCCGCTGGGA ACTCCTGTCG CCAGGAGGAT
    GTCATTGCCA CAGCCAATCT GAGTCGCCGT GCTATTGCAG ATATGCTCCG GGCTTGCAAG
    GAAGCTGCCT ACCACCCAGA AGTGGCTCCA GACGTGCGGC TCCGAGCCCT ACACTTTGGC
    CGGGAATGTG CCAATGGCTA CCTGGAACTG CTGGACCACG TGCTGCTGAC CCTGCAGAAG
    CCAAGCCCAG AATTGAAGCA GCAATTGACG GTGCACTCAA AGCGTGTGGC TGGATCAGTC
    ACTGAGCTCA TCCAGGCTGC TGAGGCCATG AAGGGAACAG AATGGGTGGA CCCAGAGGAC
    CCCACAGTCA TTGCTGAGAA TGAACTCCTG GGAGCCGCGG CTGCCATTGA GGCTGCGGCC
    AAGAAGCTGG AGCAGCTGAA GCCCCGGGCC AAACCTAAGG AGGCGGACGA GTCCTTGAAC
    TTTGAGGAGC AGATACTGGA AGCTGCCAAG TCCATCGCAG CAGCCACCAG CGCCCTGGTG
    AAGGCTGCCT CAGCTGCACA GAGGGAACTG GTGGCCCAGG GGAAGGTGGG CGCCATTCCA
    GCCAACGCAC TGGATGATGG ACAGTGGTCC CAGGGCCTCA TCTCTGCTGC CCGGATGGTG
    GCTGCGGCCA CCAACAACCT GTGTGAGGCA GCCAATGCAG CTGTCCAAGG CCACGCCAGC
    CAGGAGAAGC TCATCTCATC AGCCAAGCAG GTCGCCGCCT CCACAGCCCA GCTCCTCGTA
    GCCTGCAAGG TCAAGGCCGA CCAGGACTCT GAGGCAATGA AGCGGCTTCA GGCTGCCGGC
    AACGCAGTGA AGCGAGCCTC AGACAACCTG GTGAAGGCAG CCCAGAAGGC TGCAGCCTTT
    GAAGAACAGG AGAATGAGAC CGTGGTGGTG AAGGAGAAGA TGGTCGGGGG CATTGCCCAG
    ATCATTGCTG CCCAGGAAGA GATGCTTCGG AAGGAACGAG AGCTGGAGGA GGCACGGAAG
    AAGCTGGCGC AGATCCGGCA GCAGCAGTAC AAATTCTTAC CTTCAGAGCT TCGAGACGAG
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192357.1
    CDS319..7944
    Translation

    Target ORF information:

    RefSeq Version NM_001205428.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus talin 1 (TLN1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205428.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTTGCAC TTTCGCTGAA GATCAGCATT GGGAATGTGG TGAAGACGAT GCAATTTGAG 
    CCGTCTACCA TGGTGTATGA CGCCTGTCGG ATCATCCGTG AGCGGATCCC AGAGGCCCTA
    TCTGGCCCTC CCAGCGACTT TGGGCTGTTT CTGTCAGATG ATGACCCCAA AAAGGGTATA
    TGGCTGGAGG CAGGGAAGGC TTTGGATTAT TACATGCTCC GAAACGGGGA CACCATGGAG
    TATAGGAAGA AACAGAGGCC CTTGAAGATC CGCATGCTGG ACGGCACCGT GAAGACTGTC
    ATGGTGGACG ACTCGAAGAC TGTCACGGAC ATGCTCATGA CCATCTGTGC CCGCATTGGC
    ATCACCAACC ATGATGAATA TTCACTGGTT CGAGAGATTA TGGAAGAGAA GAAGGAGGAG
    GTGACAGGGA CCTTACGAAA GGACAAGACA CTGCTGCGAG ACGAAAAGAA GATGGAGAAA
    CTGAAGCAGA AGTTGCACAC AGATGACGAG TTGAACTGGC TGGACCATGG CCGGACCCTG
    AGGGAGCAGG GAGTGGAGGA GCACGAAACG CTTCTGCTCC GGAGGAAGTT CTTCTACTCA
    GATCAGAACG TGGACTCCCG GGACCCCGTC CAGCTGAACC TTCTCTACGT GCAGGCACGA
    GATGACATCC TGAATGGCTC CCATCCTGTC TCCTTCGACA AGGCCTGTGA GTTTGCTGGC
    TTCCAATGCC AGATCCAGTT TGGGCCCCAC AATGAGCAGA AGCACAAGGC CGGCTTCCTC
    GACCTGAAGG ACTTCCTGCC CAAGGAGTAT GTGAAGCAGA AGGGAGAGCG TAAGATCTTC
    CAGGCACACA AGAACTGTGG ACAGATGAGT GAGATTGAGG CCAAGGTACG CTACGTGAAG
    CTAGCCCGTT CCCTCAAGAC GTACGGTGTC TCCTTCTTCC TGGTCAAGGA AAAGATGAAA
    GGAAAGAACA AGTTGGTGCC CAGGCTTCTG GGCATCACTA AGGAGTGTGT GATGCGAGTG
    GACGAGAAGA CCAAGGAGGT GATCCAGGAA TGGAACCTCA CCAACATAAA ACGCTGGGCT
    GCGTCTCCCA AGAGCTTCAC CCTGGATTTT GGAGACTACC AAGATGGCTA CTACTCGGTG
    CAGACGACCG AGGGGGAGCA GATCGCTCAG CTCATCGCTG GCTACATCGA CATCATCCTT
    AAGAAGAAAA AAAGCAAGGA TCATTTTGGG CTGGAGGGAG ACGAGGAGTC TACTATGCTG
    GAGGACTCCG TGTCCCCCAA AAAGTCCACT GTGCTTCAGC AGCAGTATAA CCGGGTGGGG
    AAGGTGGAGC ATGGCTCCGT GGCCCTGCCC GCCATCATGC GCTCCGGAGC CTCGGGTCCC
    GAGAATTTCC AGGTGGGCAG CATGCCACCT GCCCAGCAGC AGATCACCAG TGGCCAGATG
    CACCGAGGAC ACATGCCTCC TTTGACGTCA GCCCAGCAGG CGCTCACTGG GACCATCAAC
    TCCAGCATGC AGGCTGTGCA AGCTGCCCAG GCCACACTGG ATGACTTTGA CACTCTGCCC
    CCTCTGGGCC AGGACGCTGC CTCAAAGGCC TGGCGTAAGA ACAAGATGGA TGAATCCAAG
    CATGAAATCC ACTCCCAGGT AGACGCCATC ACCGCTGGTA CCGCCTCTGT GGTGAACCTC
    ACAGCAGGGG ACCCTGCTGA GACAGACTAC ACGGCAGTGG GCTGTGCGGT CACCACCATC
    TCCTCCAACC TGACGGAGAT GTCCCGTGGC GTGAAGCTGC TTGCTGCCCT GCTGGAGGAT
    GAAGGCGGCA GCGGCCGGCC CCTGCTGCAG GCGGCCAAGG GCCTGGCGGG GGCGGTGTCA
    GAGCTGCTGC GCAGTGCCCA GCCAGCCAGT GCCGAGCCCC GTCAGAACCT GCTGCAAGCA
    GCTGGGAACG TGGGCCAGGC CAGTGGGGAG CTGTTGCAAC AAATTGGGGA AAGTGATACT
    GACCCCCACT TCCAGGACGT GCTCATGCAG CTAGCCAAGG CCGTGGCAAG CGCTGCAGCT
    GCCCTGGTCC TCAAGGCCAA GAGTGTGGCC CAGCGAACAG AGGACTCAGG GCTTCAGACC
    CAGGTCATCG CTGCGGCAAC ACAGTGTGCC CTGTCCACCT CCCAGCTGGT GGCCTGTACC
    AAGGTGGTGG CTCCTACTAT CAGCTCCCCA GTCTGCCAAG AACAGCTGGT AGAGGCTGGG
    CGACTAGTGG CCAAGGCTGT GGAGGGCTGT GTGTCCGCTT CCCAGGCCGC CACAGAGGAT
    GCCCAGCTGT TACGGGGGGT AGGTGCTGCG GCCACAGCTG TCACCCAGGC CCTGAATGAG
    CTGCTGCAGC ACGTGAGGGC CCATGCCACA GGGGCTGGGC CTGCTGGCCG CTACGACCAG
    GCCACTGACA CCATCCTCAC TGTCACCGAG AACATCTTCA GCTCCATGGG TGATGCTGGT
    GAGATGGTGC GACAGGCCCG CATCCTCGCC CAAGCCACCT CTGACCTGGT GAACGCCATC
    AAGGCTGATG CCGAAGGGGA GAGTGATCTG GAGAATTCCC GCAAGCTGTT AAGTGCCGCC
    AAGATCCTGG CCGATGCCAC AGCCAAGATG GTGGAGGCTG CCAAGGGAGC AGCCGCCAAT
    CCTGACAGTG AGGAGCAACA GCAGCGGCTG CGGGAGGCAG CAGAGGGTCT CCGCATGGCA
    ACCAATGCCG CTGCACAGAA CGCCATCAAG AAAAAGCTGG TGCAGCGCCT TGAGCATGCA
    GCCAAACAGG CTGCAGCCTC GGCTACACAG ACTATCGCAG CAGCCCAACA TGCAACCTCC
    ACTCCCAAGG CCTCTGCTGG CCCCCAGCCC CTGCTGGTGC AGAGCTGCAA GGCTGTGGCA
    GAGCAGATTC CACTGCTGGT GCAGGGCGTC CGAGGGAGCC AAGCGCAGCC TGACAGCCCC
    AGTGCTCAGC TAGCCCTCAT CGCTGCCAGC CAAAGCTTCC TGCAGCCAGG TGGGAAGATG
    GTGGCAGCCG CAAAGGCCTC TGTGCCAACA ATTCAGGACC AAGCATCGGC CATGCAGCTG
    AGTCAGTGTG CTAAAAACCT TGGCACCGCG TTGGCTGAAC TCCGTACTGC TGCCCAGAAG
    GCTCAGGAAG CGTGTGGGCC TTTGGAAATG GATTCTGCTC TGAGTGTGGT GCAGAATCTG
    GAGAGAGACC TGCAGGAAGT GAAGGCAGCT GCTCGAGACG GCAAGCTGAA GCCCTTGCCT
    GGGGAGACGA TGGAGAAATG TGCCCAGGAC CTGGGCAACA GCACCAAAGC CGTGAGCTCC
    GCTATTGCCC AGCTGCTGGG GGAGGTCGCC CAGGGCAATG AGAATTACGC AGGCATTGCA
    GCTCGGGATG TGGCAGGTGG GCTGCGGTCA CTGGCCCAAG CTGCTAGGGG CGTAGCTGCC
    CTGACATCAG ATCCTGCAGT GCAAGCCATT GTGCTCGACA CAGCCAGCGA TGTCCTGGAC
    AAGGCCAGCA GCCTCATCGA GGAGGCAAAG AAAGCAGCCG GCCATCCAGG GGACCCTGAG
    AGCCAGCAGC GGCTTGCCCA GGTGGCTAAA GCTGTGACCC AGGCCCTGAA CCGTTGCGTC
    AGCTGCCTGC CTGGCCAGCG TGATGTGGAC AATGCCCTGA GGGCAGTGGG AGATGCCAGC
    AAGCGACTCC TGAGTGACTC GCTTCCCCCC AGCACTGGGA CCTTTCAGGA GGCTCAGAGC
    CGGCTGAATG AAGCCGCTGC CGGGCTGAAT CAGGCAGCCA CAGAGCTGGT GCAGGCCTCT
    CGGGGAACCC CTCAGGACCT GGCCCGAGCC TCAGGTCGAT TTGGACAAGA CTTCAGCACC
    TTCCTGGAAG CCGGCGTGGA GATGGCAGGC CAGGCTCCGA GCCAGGAGGA CCGTGCCCAG
    GTCGTATCCA ACTTGAAGGG CATCTCCATG TCTTCAAGCA AACTTCTTCT CGCTGCCAAG
    GCCCTGTCCA CAGACCCTGC AGCCCCAACC CTCAAGAGTC AGCTGGCTGC AGCTGCCCGG
    GCAGTGACCG ACAGCATCAA CCAGCTCATC AGCATGTGCA CCCAGCAGGC GCCAGGCCAG
    AAGGAGTGTG ATAATGCCCT GCGGGAATTG GAGACGGTCC GGGAACTCCT AGAGAACCCA
    GTCCAGCCCA TCAATGACAT GTCCTACTTT GGCTGTCTGG ACAGCGTCAT GGAAAACTCA
    AAGGTGCTGG GTGAGGCCAT GACCGGCATC TCCCAAAATG CCAAGAACGG AAACTTGCCG
    GAGTTTGGGG AGGCTGTCGC CACAGCCTCC AAGGCCCTCT GCGGCTTCAC CGAGGCAGCT
    GCACAGGCTG CATATCTGGT TGGCGTGTCT GACCCCAACA GCCAAGCTGG ACAACAAGGG
    CTGGTGGAGC CCACACAGTT TGCCCGAGCA AACCAGGCAA TTCAGATGGC CTGTCAGAGT
    TTGGGGGAGC CTGGCTGCAC CCAGGCCCAG GTGCTCTCTG CAGCCACCAT TGTGGCCAAA
    CACACGTCTG CGCTGTGTAA CAGCTGCCGC CTGGCTTCTG CCCGAACCGC CAATCCCACT
    GCCAAGCGCC AGTTCGTACA GTCAGCCAAG GAGGTGGCCA ACAGCACGGC CAACCTTGTC
    AAGACCATCA AGGCGCTGGA TGGGGCCTTC ACAGAGGAGA ACCGTGCCCA GTGCCGAGCA
    GCAACGGCCC CTTTGCTGGA GGCTGTGGAC AATCTGAGCG CCTTTGCGTC CAACCCTGAG
    TTCTCCAGTG TTCCTGCCCA GATCAGCCCT GAGGGCCGGG CTGCCATGGA GCCCATTGTG
    ATCTCTGCTA AGACAATGTT GGAGAGTGCT GGGGGCCTTA TTCAGACAGC CCGAGCCCTG
    GCGGTCAATC CGCGGGACCC ACCACGCTGG TCCGTGCTGG CTGGCCACTC CCGTACTGTC
    TCAGACTCCA TCAAGAAGCT GATCACAAGC ATGAGGGACA AGGCCCCAGG GCAGTTGGAG
    TGTGAGACAG CCATCGCAGC TCTGAACAGC TGTCTGCGGG ACCTTGACCA GGCTTCTCTT
    GCTGCAGTCA GCCAGCAGCT CGCTCCCCGT GAGGGGATCT CTCAGGAGGC CTTGCACACC
    CAGATGCTCA CTGCGGTGCA GGAAATCTCC CATCTTATTG AGCCGCTGGC CAGCGCTGCC
    CGGGCTGAAG CCTCCCAGCT GGGACACAAG GTGTCCCAGA TGGCCCAGTA CTTTGAGCCG
    CTCACTCTGG CTGCCGTGGG TGCTGCCTCC AAAACCCTGA GCCACCCACA GCAGATGGCG
    CTCCTGGACC AGACCAAAAC CTTGGCCGAG TCTGCCCTGC AGCTGCTATA CACAGCCAAG
    GAAGCTGGCG GTAACCCCAA GCAAGCAGCT CACACCCAGG AAGCCCTGGA GGAGGCTGTG
    CAGATGATGA CAGAGGCCGT AGAGGACCTG ACAACCACCC TCAACGAGGC AGCCAGTGCT
    GCCGGGGTCG TCGGTGGCAT GGTGGACTCC ATCACCCAGG CCATCAACCA GCTAGATGAA
    GGACCAATGG GTGAACCAGA GGGTTCCTTC GTGGATTACC AGACCACCAT GGTGCGGACA
    GCCAAGGCCA TTGCTGTCAC CGTTCAGGAG ATGGTAACCA AGTCAAACAC CAGCCCTGAG
    GAGCTGGGCC CTCTCGCTAA CCAGCTGACT AGTGACTATG GGCGTCTTGC CTCCCAGGCC
    AAGCCTGCAG CTGTGGCTGC TGAAAATGAA GAGATAGGCG CCCACATCAA GCACCGGGTT
    CAGGAGCTGG GGCACGGCTG CGCAGCTCTG GTCACCAAGG CAGGCGCCCT GCAGTGTAGC
    CCTAGTGATG CCTACACCAA GAAGGAGCTC ATAGAGTGTG CCCGGAGAGT CTCTGAGAAG
    GTCTCCCACG TGCTGGCTGC GCTCCAGGCT GGGAATCGTG GCACCCAGGC CTGCATCACA
    GCCGCCAGCG CTGTGTCCGG CATCATCGCC GACCTGGACA CCACCATCAT GTTCGCCACT
    GCCGGCACAC TCAACCGTGA GGGTGCCGAA ACCTTTGCTG ACCACCGAGA GGGCATTCTG
    AAGACTGCCA AGGTGCTGGT GGAAGACACC AAGGTCCTGG TGCAGAATGC AGCTGGGAGC
    CAGGAGAAGC TGGCACAGGC AGCCCAGTCC TCCGTGGCCA CCATCACCCG CCTCGCCGAT
    GTGGTCAAGC TGGGTGCAGC CAGCCTGGGA GCTGAGGACC CAGAGACCCA GGTGGTGCTG
    ATCAATGCAG TGAAAGATGT GGCCAAGGCC CTGGGAGACC TCATCAGTGC CACAAAGGCT
    GCGGCTGGCA AAGTCGGCGA CGACCCTGCT GTGTGGCAGC TCAAGAATTC TGCCAAGGTG
    ATGGTAACCA ATGTGACATC ACTGCTCAAG ACCGTGAAAG CGGTAGAGGA TGAGGCCACC
    AAAGGCACGC GCGCCCTGGA GGCCACAACG GAGCATATTC GGCAGGAGCT GGCGGTCTTC
    TGCTCCCCAG AGCCACCTGC CAAGACCTCG ACCCCTGAAG ATTTCATCCG CATGACCAAG
    GGTATCACCA TGGCAACCGC CAAGGCCGTT GCCGCTGGGA ACTCCTGTCG CCAGGAGGAT
    GTCATTGCCA CAGCCAATCT GAGTCGCCGT GCTATTGCAG ATATGCTCCG GGCTTGCAAG
    GAAGCTGCCT ACCACCCAGA AGTGGCTCCA GACGTGCGGC TCCGAGCCCT ACACTTTGGC
    CGGGAATGTG CCAATGGCTA CCTGGAACTG CTGGACCACG TGCTGCTGAC CCTGCAGAAG
    CCAAGCCCAG AATTGAAGCA GCAATTGACG GTGCACTCAA AGCGTGTGGC TGGATCAGTC
    ACTGAGCTCA TCCAGGCTGC TGAGGCCATG AAGGGAACAG AATGGGTGGA CCCAGAGGAC
    CCCACAGTCA TTGCTGAGAA TGAACTCCTG GGAGCCGCGG CTGCCATTGA GGCTGCGGCC
    AAGAAGCTGG AGCAGCTGAA GCCCCGGGCC AAACCTAAGG AGGCGGACGA GTCCTTGAAC
    TTTGAGGAGC AGATACTGGA AGCTGCCAAG TCCATCGCAG CAGCCACCAG CGCCCTGGTG
    AAGGCTGCCT CAGCTGCACA GAGGGAACTG GTGGCCCAGG GGAAGGTGGG CGCCATTCCA
    GCCAACGCAC TGGATGATGG ACAGTGGTCC CAGGGCCTCA TCTCTGCTGC CCGGATGGTG
    GCTGCGGCCA CCAACAACCT GTGTGAGGCA GCCAATGCAG CTGTCCAAGG CCACGCCAGC
    CAGGAGAAGC TCATCTCATC AGCCAAGCAG GTCGCCGCCT CCACAGCCCA GCTCCTCGTA
    GCCTGCAAGG TCAAGGCCGA CCAGGACTCT GAGGCAATGA AGCGGCTTCA GGCTGCCGGC
    AACGCAGTGA AGCGAGCCTC AGACAACCTG GTGAAGGCAG CCCAGAAGGC TGCAGCCTTT
    GAAGAACAGG AGAATGAGAC CGTGGTGGTG AAGGAGAAGA TGGTCGGGGG CATTGCCCAG
    ATCATTGCTG CCCAGGAAGA GATGCTTCGG AAGGAACGAG AGCTGGAGGA GGCACGGAAG
    AAGCTGGCGC AGATCCGGCA GCAGCAGTAC AAATTCTTAC CTTCAGAGCT TCGAGACGAG
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22069
    Clone ID Related Accession (Same CDS sequence) XM_005210126.2 , XM_010807953.1
    Accession Version XM_005210126.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7677bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product talin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104103.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005210126.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTTGCAC TTTCGCTGAA GATCAGCATT GGGAATGTGG TGAAGACGAT GCAATTTGAG 
    CCGTCTACCA TGGTGTATGA CGCCTGTCGG ATCATCCGTG AGCGGATCCC AGAGGCCCTA
    TCTGGCCCTC CCAGCGACTT TGGGCTGTTT CTGTCAGATG ATGACCCCAA AAAGGGTATA
    TGGCTGGAGG CAGGGAAGGC TTTGGATTAT TACATGCTCC GAAACGGGGA CACCATGGAG
    TATAGGAAGA AACAGAGGCC CTTGAAGATC CGCATGCTGG ACGGCACCGT GAAGACTGTC
    ATGGTGGACG ACTCGAAGAC TGTCACGGAC ATGCTCATGA CCATCTGTGC CCGCATTGGC
    ATCACCAACC ATGATGAATA TTCACTGGTT CGAGAGATTA TGGAAGAGAA GAAGGAGGAG
    GTGACAGGGA CCTTACGAAA GGACAAGACA CTGCTGCGAG ACGAAAAGAA GATGGAGAAA
    CTGAAGCAGA AGTTGCACAC AGATGACGAG TTGAACTGGC TGGACCATGG CCGGACCCTG
    AGGGAGCAGG GAGTGGAGGA GCACGAAACG CTTCTGCTCC GGAGGAAGTT CTTCTACTCA
    GATCAGAACG TGGACTCCCG GGACCCCGTC CAGCTGAACC TTCTCTACGT GCAGGCACGA
    GATGACATCC TGAATGGCTC CCATCCTGTC TCCTTCGACA AGGCCTGTGA GTTTGCTGGC
    TTCCAATGCC AGATCCAGTT TGGGCCCCAC AATGAGCAGA AGCACAAGGC CGGCTTCCTC
    GACCTGAAGG ACTTCCTGCC CAAGGAGTAT GTGAAGCAGA AGGGAGAGCG TAAGATCTTC
    CAGGCACACA AGAACTGTGG ACAGATGAGT GAGATTGAGG CCAAGGTACG CTACGTGAAG
    CTAGCCCGTT CCCTCAAGAC GTACGGTGTC TCCTTCTTCC TGGTCAAGGA AAAGATGAAA
    GGAAAGAACA AGTTGGTGCC CAGGCTTCTG GGCATCACTA AGGAGTGTGT GATGCGAGTG
    GACGAGAAGA CCAAGGAGGT GATCCAGGAA TGGAACCTCA CCAACATAAA ACGCTGGGCT
    GCGTCTCCCA AGAGCTTCAC CCTGGATTTC GGAGACTACC AAGATGGCTA CTACTCGGTG
    CAGACGACCG AGGGGGAGCA GATCGCTCAG CTCATCGCTG GCTACATCGA CATCATCCTT
    AAGAAGAAAA AAAGCAAGGA TCATTTTGGG CTGGAGGGAG ACGAGGAGTC TACTATGCTG
    GAGGACTCCG TGTCCCCCAA AAAGTCCACT GTGCTTCAGC AGCAGTATAA CCGGGTGGGG
    AAGGTGGAGC ATGGCTCCGT GGCCCTGCCC GCCATCATGC GCTCCGGAGC CTCGGGTCCC
    GAGAATTTCC AGGTGGGCAG CATGCCACCT GCCCAGCAGC AGATCACCAG TGGCCAGATG
    CACCGAGGAC ACATGCCTCC TTTGACGTCA GCCCAGCAGG CGCTCACTGG GACCATCAAC
    TCCAGCATGC AGGCTGTGCA AGCTGCCCAG GCCACACTGG ATGACTTTGA CACTCTGCCC
    CCTCTGGGCC AGGACGCTGC CTCAAAGGCC TGGCGTAAGA ACAAGATGGA TGAATCCAAG
    CATGAAATCC ACTCCCAGGT AGACGCCATC ACCGCTGGTA CCGCCTCTGT GGTGAACCTC
    ACAGCAGGGG ACCCTGCTGA GACAGACTAC ACGGCAGTGG GCTGTGCGGT CACCACCATC
    TCCTCCAACC TGACGGAGAT GTCCCGTGGC GTGAAGCTGC TTGCTGCCCT GCTGGAGGAT
    GAAGGCGGCA GCGGCCGGCC CCTGCTGCAG GCGGCCAAGG GCCTGGCGGG GGCGGTGTCA
    GAGCTGCTGC GCAGTGCCCA GCCAGCCAGT GCCGAGCCCC GTCAGAACCT GCTGCAAGCA
    GCTGGGAACG TGGGCCAGGC CAGTGGGGAG CTGTTGCAAC AAATTGGGGA AAGTGATACT
    GACCCCCACT TCCAGATCTG TGCACCCAGC GGGGCTGGGT TTCCATCTCC CCCCGATCCC
    CCCACGGACG TGCTCATGCA GCTAGCCAAG GCCGTGGCAA GCGCTGCAGC TGCCCTGGTC
    CTCAAGGCCA AGAGTGTGGC CCAGCGAACA GAGGACTCAG GGCTTCAGAC CCAGGTCATC
    GCTGCGGCAA CACAGTGTGC CCTGTCCACC TCCCAGCTGG TGGCCTGTAC CAAGGTGGTG
    GCTCCTACTA TCAGCTCCCC AGTCTGCCAA GAACAGCTGG TAGAGGCTGG GCGACTAGTG
    GCCAAGGCTG TGGAGGGCTG TGTGTCCGCT TCCCAGGCCG CCACAGAGGA TGCCCAGCTG
    TTACGGGGGG TAGGTGCTGC GGCCACAGCT GTCACCCAGG CCCTGAATGA GCTGCTGCAG
    CACGTGAGGG CCCATGCCAC AGGGGCTGGG CCTGCTGGCC GCTACGACCA GGCCACTGAC
    ACCATCCTCA CTGTCACCTA TTTCATCTTC AGCTCCATGG GTGATGCTGG TGAGATGGTG
    CGACAGGCCC GCATCCTCGC CCAAGCCACC TCTGACCTGG TGAACGCCAT CAAGGCTGAT
    GCCGAAGGGG AGAGTGATCT GGAGAATTCC CGCAAGCTGT TAAGTGCCGC CAAGATCCTG
    GCCGATGCCA CAGCCAAGAT GGTGGAGGCT GCCAAGGGAG CAGCCGCCAA TCCTGACAGT
    GAGGAGCAAC AGCAGCGGCT GCGGGAGGCA GCAGAGGGTC TCCGCATGGC AACCAATGCC
    GCTGCACAGA ACGCCATCAA GAAAAAGCTG GTGCAGCGCC TTGAGCATGC AGCCAAACAG
    GCTGCAGCCT CGGCTACACA GACTATCGCA GCAGCCCAAC ATGCAACCTC CACTCCCAAG
    GCCTCTGCTG GCCCCCAGCC CCTGCTGGTG CAGAGCTGCA AGGCTGTGGC AGAGCAGATT
    CCACTGCTGG TGCAGGGCGT CCGAGGGAGC CAAGCGCAGC CTGACAGCCC CAGTGCTCAG
    CTAGCCCTCA TCGCTGCCAG CCAAAGCTTC CTGCAGCCAG GTGGGAAGAT GGTGGCAGCC
    GCAAAGGCCT CTGTGCCAAC AATTCAGGAC CAAGCATCGG CCATGCAGCT GAGTCAGTGT
    GCTAAAAACC TTGGCACCGC GTTGGCTGAA CTCCGTACTG CTGCCCAGAA GGCTCAGGAA
    GCGTGTGGGC CTTTGGAAAT GGATTCTGCT CTGAGTGTGG TGCAGAATCT GGAGAGAGAC
    CTGCAGGAAG TGAAGGCAGC TGCTCGAGAC GGCAAGCTGA AGCCCTTGCC TGGGGAGACG
    ATGGAGAAAT GTGCCCAGGA CCTGGGCAAC AGCACCAAAG CCGTGAGCTC CGCTATTGCC
    CAGCTGCTGG GGGAGGTCGC CCAGGGCAAT GAGAATTACG CAGGCATTGC AGCTCGGGAT
    GTGGCAGGTG GGCTGCGGTC ACTGGCCCAA GCTGCTAGGG GCGTAGCTGC CCTGACATCA
    GATCCTGCAG TGCAAGCCAT TGTGCTCGAC ACAGCCAGCG ATGTCCTGGA CAAGGCCAGC
    AGCCTCATCG AGGAGGCAAA GAAAGCAGCC GGCCATCCAG GGGACCCTGA GAGCCAGCAG
    CGGCTTGCCC AGGTGGCTAA AGCTGTGACC CAGGCCCTGA ACCGTTGCGT CAGCTGCCTG
    CCTGGCCAGC GTGATGTGGA CAATGCCCTG AGGGCAGTGG GAGATGCCAG CAAGCGACTC
    CTGAGTGACT CGCTTCCCCC CAGCACTGGG ACCTTTCAGG AGGCTCAGAG CCGGCTGAAT
    GAAGCCGCTG CCGGGCTGAA TCAGGCAGCC ACAGAGCTGG TGCAGGCCTC TCGGGGAACC
    CCTCAGGACC TGGCCCGAGC CTCAGGTCGA TTTGGACAAG ACTTCAGCAC CTTCCTGGAA
    GCCGGCGTGG AGATGGCAGG CCAGGCTCCG AGCCAGGAGG ACCGTGCCCA GGTCGTATCC
    AACTTGAAGG GCATCTCCAT GTCTTCAAGC AAACTTCTTC TCGCTGCCAA GGCCCTGTCC
    ACAGACCCTG CAGCCCCAAC CCTCAAGAGT CAGCTGGCTG CAGCTGCCCG GGCAGTGACC
    GACAGCATCA ACCAGCTCAT CAGCATGTGC ACCCAGCAGG CGCCAGGCCA GAAGGAGTGT
    GATAATGCCC TGCGGGAATT GGAGACGGTC CGGGAACTCC TAGAGAACCC AGTCCAGCCC
    ATCAATGACA TGTCCTACTT TGGCTGTCTG GACAGCGTCA TGGAAAACTC AAAGGTGCTG
    GGTGAGGCCA TGACCGGCAT CTCCCAAAAT GCCAAGAACG GAAACTTGCC GGAGTTTGGG
    GAGGCTGTCG CCACAGCCTC CAAGGCCCTC TGCGGCTTCA CCGAGGCAGC TGCACAGGCT
    GCATATCTGG TTGGCGTGTC TGACCCCAAC AGCCAAGCTG GACAACAAGG GCTGGTGGAG
    CCCACACAGT TTGCCCGAGC AAACCAGGCA ATTCAGATGG CCTGTCAGAG TTTGGGGGAG
    CCTGGCTGCA CCCAGGCCCA GGTGCTCTCT GCAGCCACCA TTGTGGCCAA ACACACGTCT
    GCGCTGTGTA ACAGCTGCCG CCTGGCTTCT GCCCGAACCG CCAATCCCAC TGCCAAGCGC
    CAGTTCGTAC AGTCAGCCAA GGAGGTGGCC AACAGCACGG CCAACCTTGT CAAGACCATC
    AAGGCGCTGG ATGGGGCCTT CACAGAGGAG AACCGTGCCC AGTGCCGAGC AGCAACGGCC
    CCTTTGCTGG AGGCTGTGGA CAATCTGAGC GCCTTTGCGT CCAACCCTGA GTTCTCCAGT
    GTTCCTGCCC AGATCAGCCC TGAGGGCCGG GCTGCCATGG AGCCCATTGT GATCTCTGCT
    AAGACAATGT TGGAGAGTGC TGGGGGCCTT ATTCAGACAG CCCGAGCCCT GGCGGTCAAT
    CCGCGGGACC CACCACGCTG GTCCGTGCTG GCTGGCCACT CCCGTACTGT CTCAGACTCC
    ATCAAGAAGC TGATCACAAG CATGAGGGAC AAGGCCCCAG GGCAGTTGGA GTGTGAGACA
    GCCATCGCAG CTCTGAACAG CTGTCTGCGG GACCTTGACC AGGCTTCTCT TGCTGCAGTC
    AGCCAGCAGC TCGCTCCCCG TGAGGGGATC TCTCAGGAGG CCTTGCACAC CCAGATGCTC
    ACTGCGGTGC AGGAAATCTC CCATCTTATT GAGCCGCTGG CCAGCGCTGC CCGGGCTGAA
    GCCTCCCAGC TGGGACACAA GGTGTCCCAG ATGGCCCAGT ACTTTGAGCC GCTCACTCTG
    GCTGCCGTGG GTGCTGCCTC CAAAACCCTG AGCCACCCAC AGCAGATGGC GCTCCTGGAC
    CAGACCAAAA CCTTGGCCGA GTCTGCCCTG CAGCTGCTAT ACACAGCCAA GGAAGCTGGC
    GGTAACCCCA AGCAAGCAGC TCACACCCAG GAAGCCCTGG AGGAGGCTGT GCAGATGATG
    ACAGAGGCCG TAGAGGACCT GACAACCACC CTCAACGAGG CAGCCAGTGC TGCCGGGGTC
    GTCGGTGGCA TGGTGGACTC CATCACCCAG GCCATCAACC AGCTAGATGA AGGACCAATG
    GGTGAACCAG AGGGTTCCTT CGTGGATTAC CAGACCACCA TGGTGCGGAC AGCCAAGGCC
    ATTGCTGTCA CCGTTCAGGA GATGGTAACC AAGTCAAACA CCAGCCCTGA GGAGCTGGGC
    CCTCTCGCTA ACCAGCTGAC TAGTGACTAT GGGCGTCTTG CCTCCCAGGC CAAGCCTGCA
    GCTGTGGCTG CTGAAAATGA AGAGATAGGC GCCCACATCA AGCACCGGGT TCAGGAGCTG
    GGGCACGGCT GCGCAGCTCT GGTCACCAAG GCAGGCGCCC TGCAGTGTAG CCCTAGTGAT
    GCCTACACCA AGAAGGAGCT CATAGAGTGT GCCCGGAGAG TCTCTGAGAA GGTCTCCCAC
    GTGCTGGCTG CGCTCCAGGC TGGGAATCGT GGCACCCAGG CCTGCATCAC AGCCGCCAGC
    GCTGTGTCCG GCATCATCGC CGACCTGGAC ACCACCATCA TGTTCGCCAC TGCCGGCACA
    CTCAACCGTG AGGGTGCCGA AACCTTTGCT GACCACCGAG AGGGCATTCT GAAGACTGCC
    AAGGTGCTGG TGGAAGACAC CAAGGTCCTG GTGCAGAATG CAGCTGGGAG CCAGGAGAAG
    CTGGCACAGG CAGCCCAGTC CTCCGTGGCC ACCATCACCC GCCTCGCCGA TGTGGTCAAG
    CTGGGTGCAG CCAGCCTGGG AGCTGAGGAC CCAGAGACCC AGGTGGTGCT GATCAATGCA
    GTGAAAGATG TGGCCAAGGC CCTGGGAGAC CTCATCAGTG CCACAAAGGC TGCGGCTGGC
    AAAGTCGGCG ACGACCCTGC TGTGTGGCAG CTCAAGAACT CTGCCAAGGT GATGGTAACC
    AATGTGACAT CACTGCTCAA GACCGTGAAA GCGGTAGAGG ATGAGGCCAC CAAAGGCACG
    CGCGCCCTGG AGGCCACAAC GGAGCATATT CGGCAGGAGC TGGCGGTCTT CTGCTCCCCA
    GAGCCACCTG CCAAGACCTC GACCCCTGAA GATTTCATCC GCATGACCAA GGGTATCACC
    ATGGCAACCG CCAAGGCCGT TGCCGCTGGG AACTCCTGTC GCCAGGAGGA TGTCATTGCC
    ACAGCCAATC TGAGTCGCCG TGCTATTGCA GATATGCTCC GGGCTTGCAA GGAAGCTGCC
    TACCACCCAG AAGTGGCTCC AGACGTGCGG CTCCGAGCCC TACACTTTGG CCGGGAATGT
    GCCAATGGCT ACCTGGAACT GCTGGACCAC GTGCTGCTGA CCCTGCAGAA GCCAAGCCCA
    GAATTGAAGC AGCAATTGAC GGTGCACTCA AAGCGTGTGG CTGGATCAGT CACTGAGCTC
    ATCCAGGCTG CTGAGGCCAT GAAGGGAACA GAATGGGTGG ACCCAGAGGA CCCCACAGTC
    ATTGCTGAGA ATGAACTCCT GGGAGCCGCG GCTGCCATTG AGGCTGCGGC CAAGAAGCTG
    GAGCAGCTGA AGCCCCGGGC CAAACCTAAG GAGGCGGACG AGTCCTTGAA CTTTGAGGAG
    CAGATACTGG AAGCTGCCAA GTCCATCGCA GCAGCCACCA GCGCCCTGGT GAAGGCTGCC
    TCAGCTGCAC AGAGGGAACT GGTGGCCCAG GGGAAGGTGG GCGCCATTCC AGCCAACGCA
    CTGGATGATG GACAGTGGTC CCAGGGCCTC ATCTCTGCTG CCCGGATGGT GGCTGCGGCC
    ACCAACAACC TGTGTGAGGC AGCCAATGCA GCTGTCCAAG GCCACGCCAG CCAGGAGAAG
    CTCATCTCAT CAGCCAAGCA GGTCGCCGCC TCCACAGCCC AGCTCCTCGT AGCCTGCAAG
    GTCAAGGCCG ACCAGGACTC TGAGGCAATG AAGCGGCTTC AGGCTGCCGG CAACGCAGTG
    AAGCGAGCCT CAGACAACCT GGTGAAGGCA GCCCAGAAGG CTGCAGCCTT TGAAGAACAG
    GAGAATGAGA CCGTGGTGGT GAAGGAGAAG ATGGTCGGGG GCATTGCCCA GATCATTGCT
    GCCCAGGAAG AGATGCTTCG GAAGGAACGA GAGCTGGAGG AGGCACGGAA GAAGCTGGCG
    CAGATCCGGC AGCAGCAGTA CAAATTCTTA CCTTCAGAGC TTCGAGACGA GCACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210183.1
    CDS314..7990
    Translation

    Target ORF information:

    RefSeq Version XM_005210126.2
    Organism Bos taurus(cattle)
    Definition Bos taurus talin 1 (TLN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210126.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTTGCAC TTTCGCTGAA GATCAGCATT GGGAATGTGG TGAAGACGAT GCAATTTGAG 
    CCGTCTACCA TGGTGTATGA CGCCTGTCGG ATCATCCGTG AGCGGATCCC AGAGGCCCTA
    TCTGGCCCTC CCAGCGACTT TGGGCTGTTT CTGTCAGATG ATGACCCCAA AAAGGGTATA
    TGGCTGGAGG CAGGGAAGGC TTTGGATTAT TACATGCTCC GAAACGGGGA CACCATGGAG
    TATAGGAAGA AACAGAGGCC CTTGAAGATC CGCATGCTGG ACGGCACCGT GAAGACTGTC
    ATGGTGGACG ACTCGAAGAC TGTCACGGAC ATGCTCATGA CCATCTGTGC CCGCATTGGC
    ATCACCAACC ATGATGAATA TTCACTGGTT CGAGAGATTA TGGAAGAGAA GAAGGAGGAG
    GTGACAGGGA CCTTACGAAA GGACAAGACA CTGCTGCGAG ACGAAAAGAA GATGGAGAAA
    CTGAAGCAGA AGTTGCACAC AGATGACGAG TTGAACTGGC TGGACCATGG CCGGACCCTG
    AGGGAGCAGG GAGTGGAGGA GCACGAAACG CTTCTGCTCC GGAGGAAGTT CTTCTACTCA
    GATCAGAACG TGGACTCCCG GGACCCCGTC CAGCTGAACC TTCTCTACGT GCAGGCACGA
    GATGACATCC TGAATGGCTC CCATCCTGTC TCCTTCGACA AGGCCTGTGA GTTTGCTGGC
    TTCCAATGCC AGATCCAGTT TGGGCCCCAC AATGAGCAGA AGCACAAGGC CGGCTTCCTC
    GACCTGAAGG ACTTCCTGCC CAAGGAGTAT GTGAAGCAGA AGGGAGAGCG TAAGATCTTC
    CAGGCACACA AGAACTGTGG ACAGATGAGT GAGATTGAGG CCAAGGTACG CTACGTGAAG
    CTAGCCCGTT CCCTCAAGAC GTACGGTGTC TCCTTCTTCC TGGTCAAGGA AAAGATGAAA
    GGAAAGAACA AGTTGGTGCC CAGGCTTCTG GGCATCACTA AGGAGTGTGT GATGCGAGTG
    GACGAGAAGA CCAAGGAGGT GATCCAGGAA TGGAACCTCA CCAACATAAA ACGCTGGGCT
    GCGTCTCCCA AGAGCTTCAC CCTGGATTTC GGAGACTACC AAGATGGCTA CTACTCGGTG
    CAGACGACCG AGGGGGAGCA GATCGCTCAG CTCATCGCTG GCTACATCGA CATCATCCTT
    AAGAAGAAAA AAAGCAAGGA TCATTTTGGG CTGGAGGGAG ACGAGGAGTC TACTATGCTG
    GAGGACTCCG TGTCCCCCAA AAAGTCCACT GTGCTTCAGC AGCAGTATAA CCGGGTGGGG
    AAGGTGGAGC ATGGCTCCGT GGCCCTGCCC GCCATCATGC GCTCCGGAGC CTCGGGTCCC
    GAGAATTTCC AGGTGGGCAG CATGCCACCT GCCCAGCAGC AGATCACCAG TGGCCAGATG
    CACCGAGGAC ACATGCCTCC TTTGACGTCA GCCCAGCAGG CGCTCACTGG GACCATCAAC
    TCCAGCATGC AGGCTGTGCA AGCTGCCCAG GCCACACTGG ATGACTTTGA CACTCTGCCC
    CCTCTGGGCC AGGACGCTGC CTCAAAGGCC TGGCGTAAGA ACAAGATGGA TGAATCCAAG
    CATGAAATCC ACTCCCAGGT AGACGCCATC ACCGCTGGTA CCGCCTCTGT GGTGAACCTC
    ACAGCAGGGG ACCCTGCTGA GACAGACTAC ACGGCAGTGG GCTGTGCGGT CACCACCATC
    TCCTCCAACC TGACGGAGAT GTCCCGTGGC GTGAAGCTGC TTGCTGCCCT GCTGGAGGAT
    GAAGGCGGCA GCGGCCGGCC CCTGCTGCAG GCGGCCAAGG GCCTGGCGGG GGCGGTGTCA
    GAGCTGCTGC GCAGTGCCCA GCCAGCCAGT GCCGAGCCCC GTCAGAACCT GCTGCAAGCA
    GCTGGGAACG TGGGCCAGGC CAGTGGGGAG CTGTTGCAAC AAATTGGGGA AAGTGATACT
    GACCCCCACT TCCAGATCTG TGCACCCAGC GGGGCTGGGT TTCCATCTCC CCCCGATCCC
    CCCACGGACG TGCTCATGCA GCTAGCCAAG GCCGTGGCAA GCGCTGCAGC TGCCCTGGTC
    CTCAAGGCCA AGAGTGTGGC CCAGCGAACA GAGGACTCAG GGCTTCAGAC CCAGGTCATC
    GCTGCGGCAA CACAGTGTGC CCTGTCCACC TCCCAGCTGG TGGCCTGTAC CAAGGTGGTG
    GCTCCTACTA TCAGCTCCCC AGTCTGCCAA GAACAGCTGG TAGAGGCTGG GCGACTAGTG
    GCCAAGGCTG TGGAGGGCTG TGTGTCCGCT TCCCAGGCCG CCACAGAGGA TGCCCAGCTG
    TTACGGGGGG TAGGTGCTGC GGCCACAGCT GTCACCCAGG CCCTGAATGA GCTGCTGCAG
    CACGTGAGGG CCCATGCCAC AGGGGCTGGG CCTGCTGGCC GCTACGACCA GGCCACTGAC
    ACCATCCTCA CTGTCACCTA TTTCATCTTC AGCTCCATGG GTGATGCTGG TGAGATGGTG
    CGACAGGCCC GCATCCTCGC CCAAGCCACC TCTGACCTGG TGAACGCCAT CAAGGCTGAT
    GCCGAAGGGG AGAGTGATCT GGAGAATTCC CGCAAGCTGT TAAGTGCCGC CAAGATCCTG
    GCCGATGCCA CAGCCAAGAT GGTGGAGGCT GCCAAGGGAG CAGCCGCCAA TCCTGACAGT
    GAGGAGCAAC AGCAGCGGCT GCGGGAGGCA GCAGAGGGTC TCCGCATGGC AACCAATGCC
    GCTGCACAGA ACGCCATCAA GAAAAAGCTG GTGCAGCGCC TTGAGCATGC AGCCAAACAG
    GCTGCAGCCT CGGCTACACA GACTATCGCA GCAGCCCAAC ATGCAACCTC CACTCCCAAG
    GCCTCTGCTG GCCCCCAGCC CCTGCTGGTG CAGAGCTGCA AGGCTGTGGC AGAGCAGATT
    CCACTGCTGG TGCAGGGCGT CCGAGGGAGC CAAGCGCAGC CTGACAGCCC CAGTGCTCAG
    CTAGCCCTCA TCGCTGCCAG CCAAAGCTTC CTGCAGCCAG GTGGGAAGAT GGTGGCAGCC
    GCAAAGGCCT CTGTGCCAAC AATTCAGGAC CAAGCATCGG CCATGCAGCT GAGTCAGTGT
    GCTAAAAACC TTGGCACCGC GTTGGCTGAA CTCCGTACTG CTGCCCAGAA GGCTCAGGAA
    GCGTGTGGGC CTTTGGAAAT GGATTCTGCT CTGAGTGTGG TGCAGAATCT GGAGAGAGAC
    CTGCAGGAAG TGAAGGCAGC TGCTCGAGAC GGCAAGCTGA AGCCCTTGCC TGGGGAGACG
    ATGGAGAAAT GTGCCCAGGA CCTGGGCAAC AGCACCAAAG CCGTGAGCTC CGCTATTGCC
    CAGCTGCTGG GGGAGGTCGC CCAGGGCAAT GAGAATTACG CAGGCATTGC AGCTCGGGAT
    GTGGCAGGTG GGCTGCGGTC ACTGGCCCAA GCTGCTAGGG GCGTAGCTGC CCTGACATCA
    GATCCTGCAG TGCAAGCCAT TGTGCTCGAC ACAGCCAGCG ATGTCCTGGA CAAGGCCAGC
    AGCCTCATCG AGGAGGCAAA GAAAGCAGCC GGCCATCCAG GGGACCCTGA GAGCCAGCAG
    CGGCTTGCCC AGGTGGCTAA AGCTGTGACC CAGGCCCTGA ACCGTTGCGT CAGCTGCCTG
    CCTGGCCAGC GTGATGTGGA CAATGCCCTG AGGGCAGTGG GAGATGCCAG CAAGCGACTC
    CTGAGTGACT CGCTTCCCCC CAGCACTGGG ACCTTTCAGG AGGCTCAGAG CCGGCTGAAT
    GAAGCCGCTG CCGGGCTGAA TCAGGCAGCC ACAGAGCTGG TGCAGGCCTC TCGGGGAACC
    CCTCAGGACC TGGCCCGAGC CTCAGGTCGA TTTGGACAAG ACTTCAGCAC CTTCCTGGAA
    GCCGGCGTGG AGATGGCAGG CCAGGCTCCG AGCCAGGAGG ACCGTGCCCA GGTCGTATCC
    AACTTGAAGG GCATCTCCAT GTCTTCAAGC AAACTTCTTC TCGCTGCCAA GGCCCTGTCC
    ACAGACCCTG CAGCCCCAAC CCTCAAGAGT CAGCTGGCTG CAGCTGCCCG GGCAGTGACC
    GACAGCATCA ACCAGCTCAT CAGCATGTGC ACCCAGCAGG CGCCAGGCCA GAAGGAGTGT
    GATAATGCCC TGCGGGAATT GGAGACGGTC CGGGAACTCC TAGAGAACCC AGTCCAGCCC
    ATCAATGACA TGTCCTACTT TGGCTGTCTG GACAGCGTCA TGGAAAACTC AAAGGTGCTG
    GGTGAGGCCA TGACCGGCAT CTCCCAAAAT GCCAAGAACG GAAACTTGCC GGAGTTTGGG
    GAGGCTGTCG CCACAGCCTC CAAGGCCCTC TGCGGCTTCA CCGAGGCAGC TGCACAGGCT
    GCATATCTGG TTGGCGTGTC TGACCCCAAC AGCCAAGCTG GACAACAAGG GCTGGTGGAG
    CCCACACAGT TTGCCCGAGC AAACCAGGCA ATTCAGATGG CCTGTCAGAG TTTGGGGGAG
    CCTGGCTGCA CCCAGGCCCA GGTGCTCTCT GCAGCCACCA TTGTGGCCAA ACACACGTCT
    GCGCTGTGTA ACAGCTGCCG CCTGGCTTCT GCCCGAACCG CCAATCCCAC TGCCAAGCGC
    CAGTTCGTAC AGTCAGCCAA GGAGGTGGCC AACAGCACGG CCAACCTTGT CAAGACCATC
    AAGGCGCTGG ATGGGGCCTT CACAGAGGAG AACCGTGCCC AGTGCCGAGC AGCAACGGCC
    CCTTTGCTGG AGGCTGTGGA CAATCTGAGC GCCTTTGCGT CCAACCCTGA GTTCTCCAGT
    GTTCCTGCCC AGATCAGCCC TGAGGGCCGG GCTGCCATGG AGCCCATTGT GATCTCTGCT
    AAGACAATGT TGGAGAGTGC TGGGGGCCTT ATTCAGACAG CCCGAGCCCT GGCGGTCAAT
    CCGCGGGACC CACCACGCTG GTCCGTGCTG GCTGGCCACT CCCGTACTGT CTCAGACTCC
    ATCAAGAAGC TGATCACAAG CATGAGGGAC AAGGCCCCAG GGCAGTTGGA GTGTGAGACA
    GCCATCGCAG CTCTGAACAG CTGTCTGCGG GACCTTGACC AGGCTTCTCT TGCTGCAGTC
    AGCCAGCAGC TCGCTCCCCG TGAGGGGATC TCTCAGGAGG CCTTGCACAC CCAGATGCTC
    ACTGCGGTGC AGGAAATCTC CCATCTTATT GAGCCGCTGG CCAGCGCTGC CCGGGCTGAA
    GCCTCCCAGC TGGGACACAA GGTGTCCCAG ATGGCCCAGT ACTTTGAGCC GCTCACTCTG
    GCTGCCGTGG GTGCTGCCTC CAAAACCCTG AGCCACCCAC AGCAGATGGC GCTCCTGGAC
    CAGACCAAAA CCTTGGCCGA GTCTGCCCTG CAGCTGCTAT ACACAGCCAA GGAAGCTGGC
    GGTAACCCCA AGCAAGCAGC TCACACCCAG GAAGCCCTGG AGGAGGCTGT GCAGATGATG
    ACAGAGGCCG TAGAGGACCT GACAACCACC CTCAACGAGG CAGCCAGTGC TGCCGGGGTC
    GTCGGTGGCA TGGTGGACTC CATCACCCAG GCCATCAACC AGCTAGATGA AGGACCAATG
    GGTGAACCAG AGGGTTCCTT CGTGGATTAC CAGACCACCA TGGTGCGGAC AGCCAAGGCC
    ATTGCTGTCA CCGTTCAGGA GATGGTAACC AAGTCAAACA CCAGCCCTGA GGAGCTGGGC
    CCTCTCGCTA ACCAGCTGAC TAGTGACTAT GGGCGTCTTG CCTCCCAGGC CAAGCCTGCA
    GCTGTGGCTG CTGAAAATGA AGAGATAGGC GCCCACATCA AGCACCGGGT TCAGGAGCTG
    GGGCACGGCT GCGCAGCTCT GGTCACCAAG GCAGGCGCCC TGCAGTGTAG CCCTAGTGAT
    GCCTACACCA AGAAGGAGCT CATAGAGTGT GCCCGGAGAG TCTCTGAGAA GGTCTCCCAC
    GTGCTGGCTG CGCTCCAGGC TGGGAATCGT GGCACCCAGG CCTGCATCAC AGCCGCCAGC
    GCTGTGTCCG GCATCATCGC CGACCTGGAC ACCACCATCA TGTTCGCCAC TGCCGGCACA
    CTCAACCGTG AGGGTGCCGA AACCTTTGCT GACCACCGAG AGGGCATTCT GAAGACTGCC
    AAGGTGCTGG TGGAAGACAC CAAGGTCCTG GTGCAGAATG CAGCTGGGAG CCAGGAGAAG
    CTGGCACAGG CAGCCCAGTC CTCCGTGGCC ACCATCACCC GCCTCGCCGA TGTGGTCAAG
    CTGGGTGCAG CCAGCCTGGG AGCTGAGGAC CCAGAGACCC AGGTGGTGCT GATCAATGCA
    GTGAAAGATG TGGCCAAGGC CCTGGGAGAC CTCATCAGTG CCACAAAGGC TGCGGCTGGC
    AAAGTCGGCG ACGACCCTGC TGTGTGGCAG CTCAAGAACT CTGCCAAGGT GATGGTAACC
    AATGTGACAT CACTGCTCAA GACCGTGAAA GCGGTAGAGG ATGAGGCCAC CAAAGGCACG
    CGCGCCCTGG AGGCCACAAC GGAGCATATT CGGCAGGAGC TGGCGGTCTT CTGCTCCCCA
    GAGCCACCTG CCAAGACCTC GACCCCTGAA GATTTCATCC GCATGACCAA GGGTATCACC
    ATGGCAACCG CCAAGGCCGT TGCCGCTGGG AACTCCTGTC GCCAGGAGGA TGTCATTGCC
    ACAGCCAATC TGAGTCGCCG TGCTATTGCA GATATGCTCC GGGCTTGCAA GGAAGCTGCC
    TACCACCCAG AAGTGGCTCC AGACGTGCGG CTCCGAGCCC TACACTTTGG CCGGGAATGT
    GCCAATGGCT ACCTGGAACT GCTGGACCAC GTGCTGCTGA CCCTGCAGAA GCCAAGCCCA
    GAATTGAAGC AGCAATTGAC GGTGCACTCA AAGCGTGTGG CTGGATCAGT CACTGAGCTC
    ATCCAGGCTG CTGAGGCCAT GAAGGGAACA GAATGGGTGG ACCCAGAGGA CCCCACAGTC
    ATTGCTGAGA ATGAACTCCT GGGAGCCGCG GCTGCCATTG AGGCTGCGGC CAAGAAGCTG
    GAGCAGCTGA AGCCCCGGGC CAAACCTAAG GAGGCGGACG AGTCCTTGAA CTTTGAGGAG
    CAGATACTGG AAGCTGCCAA GTCCATCGCA GCAGCCACCA GCGCCCTGGT GAAGGCTGCC
    TCAGCTGCAC AGAGGGAACT GGTGGCCCAG GGGAAGGTGG GCGCCATTCC AGCCAACGCA
    CTGGATGATG GACAGTGGTC CCAGGGCCTC ATCTCTGCTG CCCGGATGGT GGCTGCGGCC
    ACCAACAACC TGTGTGAGGC AGCCAATGCA GCTGTCCAAG GCCACGCCAG CCAGGAGAAG
    CTCATCTCAT CAGCCAAGCA GGTCGCCGCC TCCACAGCCC AGCTCCTCGT AGCCTGCAAG
    GTCAAGGCCG ACCAGGACTC TGAGGCAATG AAGCGGCTTC AGGCTGCCGG CAACGCAGTG
    AAGCGAGCCT CAGACAACCT GGTGAAGGCA GCCCAGAAGG CTGCAGCCTT TGAAGAACAG
    GAGAATGAGA CCGTGGTGGT GAAGGAGAAG ATGGTCGGGG GCATTGCCCA GATCATTGCT
    GCCCAGGAAG AGATGCTTCG GAAGGAACGA GAGCTGGAGG AGGCACGGAA GAAGCTGGCG
    CAGATCCGGC AGCAGCAGTA CAAATTCTTA CCTTCAGAGC TTCGAGACGA GCACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22069
    Clone ID Related Accession (Same CDS sequence) XM_005210126.2 , XM_010807953.1
    Accession Version XM_010807953.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7677bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product talin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTTGCAC TTTCGCTGAA GATCAGCATT GGGAATGTGG TGAAGACGAT GCAATTTGAG 
    CCGTCTACCA TGGTGTATGA CGCCTGTCGG ATCATCCGTG AGCGGATCCC AGAGGCCCTA
    TCTGGCCCTC CCAGCGACTT TGGGCTGTTT CTGTCAGATG ATGACCCCAA AAAGGGTATA
    TGGCTGGAGG CAGGGAAGGC TTTGGATTAT TACATGCTCC GAAACGGGGA CACCATGGAG
    TATAGGAAGA AACAGAGGCC CTTGAAGATC CGCATGCTGG ACGGCACCGT GAAGACTGTC
    ATGGTGGACG ACTCGAAGAC TGTCACGGAC ATGCTCATGA CCATCTGTGC CCGCATTGGC
    ATCACCAACC ATGATGAATA TTCACTGGTT CGAGAGATTA TGGAAGAGAA GAAGGAGGAG
    GTGACAGGGA CCTTACGAAA GGACAAGACA CTGCTGCGAG ACGAAAAGAA GATGGAGAAA
    CTGAAGCAGA AGTTGCACAC AGATGACGAG TTGAACTGGC TGGACCATGG CCGGACCCTG
    AGGGAGCAGG GAGTGGAGGA GCACGAAACG CTTCTGCTCC GGAGGAAGTT CTTCTACTCA
    GATCAGAACG TGGACTCCCG GGACCCCGTC CAGCTGAACC TTCTCTACGT GCAGGCACGA
    GATGACATCC TGAATGGCTC CCATCCTGTC TCCTTCGACA AGGCCTGTGA GTTTGCTGGC
    TTCCAATGCC AGATCCAGTT TGGGCCCCAC AATGAGCAGA AGCACAAGGC CGGCTTCCTC
    GACCTGAAGG ACTTCCTGCC CAAGGAGTAT GTGAAGCAGA AGGGAGAGCG TAAGATCTTC
    CAGGCACACA AGAACTGTGG ACAGATGAGT GAGATTGAGG CCAAGGTACG CTACGTGAAG
    CTAGCCCGTT CCCTCAAGAC GTACGGTGTC TCCTTCTTCC TGGTCAAGGA AAAGATGAAA
    GGAAAGAACA AGTTGGTGCC CAGGCTTCTG GGCATCACTA AGGAGTGTGT GATGCGAGTG
    GACGAGAAGA CCAAGGAGGT GATCCAGGAA TGGAACCTCA CCAACATAAA ACGCTGGGCT
    GCGTCTCCCA AGAGCTTCAC CCTGGATTTC GGAGACTACC AAGATGGCTA CTACTCGGTG
    CAGACGACCG AGGGGGAGCA GATCGCTCAG CTCATCGCTG GCTACATCGA CATCATCCTT
    AAGAAGAAAA AAAGCAAGGA TCATTTTGGG CTGGAGGGAG ACGAGGAGTC TACTATGCTG
    GAGGACTCCG TGTCCCCCAA AAAGTCCACT GTGCTTCAGC AGCAGTATAA CCGGGTGGGG
    AAGGTGGAGC ATGGCTCCGT GGCCCTGCCC GCCATCATGC GCTCCGGAGC CTCGGGTCCC
    GAGAATTTCC AGGTGGGCAG CATGCCACCT GCCCAGCAGC AGATCACCAG TGGCCAGATG
    CACCGAGGAC ACATGCCTCC TTTGACGTCA GCCCAGCAGG CGCTCACTGG GACCATCAAC
    TCCAGCATGC AGGCTGTGCA AGCTGCCCAG GCCACACTGG ATGACTTTGA CACTCTGCCC
    CCTCTGGGCC AGGACGCTGC CTCAAAGGCC TGGCGTAAGA ACAAGATGGA TGAATCCAAG
    CATGAAATCC ACTCCCAGGT AGACGCCATC ACCGCTGGTA CCGCCTCTGT GGTGAACCTC
    ACAGCAGGGG ACCCTGCTGA GACAGACTAC ACGGCAGTGG GCTGTGCGGT CACCACCATC
    TCCTCCAACC TGACGGAGAT GTCCCGTGGC GTGAAGCTGC TTGCTGCCCT GCTGGAGGAT
    GAAGGCGGCA GCGGCCGGCC CCTGCTGCAG GCGGCCAAGG GCCTGGCGGG GGCGGTGTCA
    GAGCTGCTGC GCAGTGCCCA GCCAGCCAGT GCCGAGCCCC GTCAGAACCT GCTGCAAGCA
    GCTGGGAACG TGGGCCAGGC CAGTGGGGAG CTGTTGCAAC AAATTGGGGA AAGTGATACT
    GACCCCCACT TCCAGATCTG TGCACCCAGC GGGGCTGGGT TTCCATCTCC CCCCGATCCC
    CCCACGGACG TGCTCATGCA GCTAGCCAAG GCCGTGGCAA GCGCTGCAGC TGCCCTGGTC
    CTCAAGGCCA AGAGTGTGGC CCAGCGAACA GAGGACTCAG GGCTTCAGAC CCAGGTCATC
    GCTGCGGCAA CACAGTGTGC CCTGTCCACC TCCCAGCTGG TGGCCTGTAC CAAGGTGGTG
    GCTCCTACTA TCAGCTCCCC AGTCTGCCAA GAACAGCTGG TAGAGGCTGG GCGACTAGTG
    GCCAAGGCTG TGGAGGGCTG TGTGTCCGCT TCCCAGGCCG CCACAGAGGA TGCCCAGCTG
    TTACGGGGGG TAGGTGCTGC GGCCACAGCT GTCACCCAGG CCCTGAATGA GCTGCTGCAG
    CACGTGAGGG CCCATGCCAC AGGGGCTGGG CCTGCTGGCC GCTACGACCA GGCCACTGAC
    ACCATCCTCA CTGTCACCTA TTTCATCTTC AGCTCCATGG GTGATGCTGG TGAGATGGTG
    CGACAGGCCC GCATCCTCGC CCAAGCCACC TCTGACCTGG TGAACGCCAT CAAGGCTGAT
    GCCGAAGGGG AGAGTGATCT GGAGAATTCC CGCAAGCTGT TAAGTGCCGC CAAGATCCTG
    GCCGATGCCA CAGCCAAGAT GGTGGAGGCT GCCAAGGGAG CAGCCGCCAA TCCTGACAGT
    GAGGAGCAAC AGCAGCGGCT GCGGGAGGCA GCAGAGGGTC TCCGCATGGC AACCAATGCC
    GCTGCACAGA ACGCCATCAA GAAAAAGCTG GTGCAGCGCC TTGAGCATGC AGCCAAACAG
    GCTGCAGCCT CGGCTACACA GACTATCGCA GCAGCCCAAC ATGCAACCTC CACTCCCAAG
    GCCTCTGCTG GCCCCCAGCC CCTGCTGGTG CAGAGCTGCA AGGCTGTGGC AGAGCAGATT
    CCACTGCTGG TGCAGGGCGT CCGAGGGAGC CAAGCGCAGC CTGACAGCCC CAGTGCTCAG
    CTAGCCCTCA TCGCTGCCAG CCAAAGCTTC CTGCAGCCAG GTGGGAAGAT GGTGGCAGCC
    GCAAAGGCCT CTGTGCCAAC AATTCAGGAC CAAGCATCGG CCATGCAGCT GAGTCAGTGT
    GCTAAAAACC TTGGCACCGC GTTGGCTGAA CTCCGTACTG CTGCCCAGAA GGCTCAGGAA
    GCGTGTGGGC CTTTGGAAAT GGATTCTGCT CTGAGTGTGG TGCAGAATCT GGAGAGAGAC
    CTGCAGGAAG TGAAGGCAGC TGCTCGAGAC GGCAAGCTGA AGCCCTTGCC TGGGGAGACG
    ATGGAGAAAT GTGCCCAGGA CCTGGGCAAC AGCACCAAAG CCGTGAGCTC CGCTATTGCC
    CAGCTGCTGG GGGAGGTCGC CCAGGGCAAT GAGAATTACG CAGGCATTGC AGCTCGGGAT
    GTGGCAGGTG GGCTGCGGTC ACTGGCCCAA GCTGCTAGGG GCGTAGCTGC CCTGACATCA
    GATCCTGCAG TGCAAGCCAT TGTGCTCGAC ACAGCCAGCG ATGTCCTGGA CAAGGCCAGC
    AGCCTCATCG AGGAGGCAAA GAAAGCAGCC GGCCATCCAG GGGACCCTGA GAGCCAGCAG
    CGGCTTGCCC AGGTGGCTAA AGCTGTGACC CAGGCCCTGA ACCGTTGCGT CAGCTGCCTG
    CCTGGCCAGC GTGATGTGGA CAATGCCCTG AGGGCAGTGG GAGATGCCAG CAAGCGACTC
    CTGAGTGACT CGCTTCCCCC CAGCACTGGG ACCTTTCAGG AGGCTCAGAG CCGGCTGAAT
    GAAGCCGCTG CCGGGCTGAA TCAGGCAGCC ACAGAGCTGG TGCAGGCCTC TCGGGGAACC
    CCTCAGGACC TGGCCCGAGC CTCAGGTCGA TTTGGACAAG ACTTCAGCAC CTTCCTGGAA
    GCCGGCGTGG AGATGGCAGG CCAGGCTCCG AGCCAGGAGG ACCGTGCCCA GGTCGTATCC
    AACTTGAAGG GCATCTCCAT GTCTTCAAGC AAACTTCTTC TCGCTGCCAA GGCCCTGTCC
    ACAGACCCTG CAGCCCCAAC CCTCAAGAGT CAGCTGGCTG CAGCTGCCCG GGCAGTGACC
    GACAGCATCA ACCAGCTCAT CAGCATGTGC ACCCAGCAGG CGCCAGGCCA GAAGGAGTGT
    GATAATGCCC TGCGGGAATT GGAGACGGTC CGGGAACTCC TAGAGAACCC AGTCCAGCCC
    ATCAATGACA TGTCCTACTT TGGCTGTCTG GACAGCGTCA TGGAAAACTC AAAGGTGCTG
    GGTGAGGCCA TGACCGGCAT CTCCCAAAAT GCCAAGAACG GAAACTTGCC GGAGTTTGGG
    GAGGCTGTCG CCACAGCCTC CAAGGCCCTC TGCGGCTTCA CCGAGGCAGC TGCACAGGCT
    GCATATCTGG TTGGCGTGTC TGACCCCAAC AGCCAAGCTG GACAACAAGG GCTGGTGGAG
    CCCACACAGT TTGCCCGAGC AAACCAGGCA ATTCAGATGG CCTGTCAGAG TTTGGGGGAG
    CCTGGCTGCA CCCAGGCCCA GGTGCTCTCT GCAGCCACCA TTGTGGCCAA ACACACGTCT
    GCGCTGTGTA ACAGCTGCCG CCTGGCTTCT GCCCGAACCG CCAATCCCAC TGCCAAGCGC
    CAGTTCGTAC AGTCAGCCAA GGAGGTGGCC AACAGCACGG CCAACCTTGT CAAGACCATC
    AAGGCGCTGG ATGGGGCCTT CACAGAGGAG AACCGTGCCC AGTGCCGAGC AGCAACGGCC
    CCTTTGCTGG AGGCTGTGGA CAATCTGAGC GCCTTTGCGT CCAACCCTGA GTTCTCCAGT
    GTTCCTGCCC AGATCAGCCC TGAGGGCCGG GCTGCCATGG AGCCCATTGT GATCTCTGCT
    AAGACAATGT TGGAGAGTGC TGGGGGCCTT ATTCAGACAG CCCGAGCCCT GGCGGTCAAT
    CCGCGGGACC CACCACGCTG GTCCGTGCTG GCTGGCCACT CCCGTACTGT CTCAGACTCC
    ATCAAGAAGC TGATCACAAG CATGAGGGAC AAGGCCCCAG GGCAGTTGGA GTGTGAGACA
    GCCATCGCAG CTCTGAACAG CTGTCTGCGG GACCTTGACC AGGCTTCTCT TGCTGCAGTC
    AGCCAGCAGC TCGCTCCCCG TGAGGGGATC TCTCAGGAGG CCTTGCACAC CCAGATGCTC
    ACTGCGGTGC AGGAAATCTC CCATCTTATT GAGCCGCTGG CCAGCGCTGC CCGGGCTGAA
    GCCTCCCAGC TGGGACACAA GGTGTCCCAG ATGGCCCAGT ACTTTGAGCC GCTCACTCTG
    GCTGCCGTGG GTGCTGCCTC CAAAACCCTG AGCCACCCAC AGCAGATGGC GCTCCTGGAC
    CAGACCAAAA CCTTGGCCGA GTCTGCCCTG CAGCTGCTAT ACACAGCCAA GGAAGCTGGC
    GGTAACCCCA AGCAAGCAGC TCACACCCAG GAAGCCCTGG AGGAGGCTGT GCAGATGATG
    ACAGAGGCCG TAGAGGACCT GACAACCACC CTCAACGAGG CAGCCAGTGC TGCCGGGGTC
    GTCGGTGGCA TGGTGGACTC CATCACCCAG GCCATCAACC AGCTAGATGA AGGACCAATG
    GGTGAACCAG AGGGTTCCTT CGTGGATTAC CAGACCACCA TGGTGCGGAC AGCCAAGGCC
    ATTGCTGTCA CCGTTCAGGA GATGGTAACC AAGTCAAACA CCAGCCCTGA GGAGCTGGGC
    CCTCTCGCTA ACCAGCTGAC TAGTGACTAT GGGCGTCTTG CCTCCCAGGC CAAGCCTGCA
    GCTGTGGCTG CTGAAAATGA AGAGATAGGC GCCCACATCA AGCACCGGGT TCAGGAGCTG
    GGGCACGGCT GCGCAGCTCT GGTCACCAAG GCAGGCGCCC TGCAGTGTAG CCCTAGTGAT
    GCCTACACCA AGAAGGAGCT CATAGAGTGT GCCCGGAGAG TCTCTGAGAA GGTCTCCCAC
    GTGCTGGCTG CGCTCCAGGC TGGGAATCGT GGCACCCAGG CCTGCATCAC AGCCGCCAGC
    GCTGTGTCCG GCATCATCGC CGACCTGGAC ACCACCATCA TGTTCGCCAC TGCCGGCACA
    CTCAACCGTG AGGGTGCCGA AACCTTTGCT GACCACCGAG AGGGCATTCT GAAGACTGCC
    AAGGTGCTGG TGGAAGACAC CAAGGTCCTG GTGCAGAATG CAGCTGGGAG CCAGGAGAAG
    CTGGCACAGG CAGCCCAGTC CTCCGTGGCC ACCATCACCC GCCTCGCCGA TGTGGTCAAG
    CTGGGTGCAG CCAGCCTGGG AGCTGAGGAC CCAGAGACCC AGGTGGTGCT GATCAATGCA
    GTGAAAGATG TGGCCAAGGC CCTGGGAGAC CTCATCAGTG CCACAAAGGC TGCGGCTGGC
    AAAGTCGGCG ACGACCCTGC TGTGTGGCAG CTCAAGAACT CTGCCAAGGT GATGGTAACC
    AATGTGACAT CACTGCTCAA GACCGTGAAA GCGGTAGAGG ATGAGGCCAC CAAAGGCACG
    CGCGCCCTGG AGGCCACAAC GGAGCATATT CGGCAGGAGC TGGCGGTCTT CTGCTCCCCA
    GAGCCACCTG CCAAGACCTC GACCCCTGAA GATTTCATCC GCATGACCAA GGGTATCACC
    ATGGCAACCG CCAAGGCCGT TGCCGCTGGG AACTCCTGTC GCCAGGAGGA TGTCATTGCC
    ACAGCCAATC TGAGTCGCCG TGCTATTGCA GATATGCTCC GGGCTTGCAA GGAAGCTGCC
    TACCACCCAG AAGTGGCTCC AGACGTGCGG CTCCGAGCCC TACACTTTGG CCGGGAATGT
    GCCAATGGCT ACCTGGAACT GCTGGACCAC GTGCTGCTGA CCCTGCAGAA GCCAAGCCCA
    GAATTGAAGC AGCAATTGAC GGTGCACTCA AAGCGTGTGG CTGGATCAGT CACTGAGCTC
    ATCCAGGCTG CTGAGGCCAT GAAGGGAACA GAATGGGTGG ACCCAGAGGA CCCCACAGTC
    ATTGCTGAGA ATGAACTCCT GGGAGCCGCG GCTGCCATTG AGGCTGCGGC CAAGAAGCTG
    GAGCAGCTGA AGCCCCGGGC CAAACCTAAG GAGGCGGACG AGTCCTTGAA CTTTGAGGAG
    CAGATACTGG AAGCTGCCAA GTCCATCGCA GCAGCCACCA GCGCCCTGGT GAAGGCTGCC
    TCAGCTGCAC AGAGGGAACT GGTGGCCCAG GGGAAGGTGG GCGCCATTCC AGCCAACGCA
    CTGGATGATG GACAGTGGTC CCAGGGCCTC ATCTCTGCTG CCCGGATGGT GGCTGCGGCC
    ACCAACAACC TGTGTGAGGC AGCCAATGCA GCTGTCCAAG GCCACGCCAG CCAGGAGAAG
    CTCATCTCAT CAGCCAAGCA GGTCGCCGCC TCCACAGCCC AGCTCCTCGT AGCCTGCAAG
    GTCAAGGCCG ACCAGGACTC TGAGGCAATG AAGCGGCTTC AGGCTGCCGG CAACGCAGTG
    AAGCGAGCCT CAGACAACCT GGTGAAGGCA GCCCAGAAGG CTGCAGCCTT TGAAGAACAG
    GAGAATGAGA CCGTGGTGGT GAAGGAGAAG ATGGTCGGGG GCATTGCCCA GATCATTGCT
    GCCCAGGAAG AGATGCTTCG GAAGGAACGA GAGCTGGAGG AGGCACGGAA GAAGCTGGCG
    CAGATCCGGC AGCAGCAGTA CAAATTCTTA CCTTCAGAGC TTCGAGACGA GCACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806255.1
    CDS197..7873
    Translation

    Target ORF information:

    RefSeq Version XM_010807953.1
    Organism Bos taurus(cattle)
    Definition Bos taurus talin 1 (TLN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807953.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTTGCAC TTTCGCTGAA GATCAGCATT GGGAATGTGG TGAAGACGAT GCAATTTGAG 
    CCGTCTACCA TGGTGTATGA CGCCTGTCGG ATCATCCGTG AGCGGATCCC AGAGGCCCTA
    TCTGGCCCTC CCAGCGACTT TGGGCTGTTT CTGTCAGATG ATGACCCCAA AAAGGGTATA
    TGGCTGGAGG CAGGGAAGGC TTTGGATTAT TACATGCTCC GAAACGGGGA CACCATGGAG
    TATAGGAAGA AACAGAGGCC CTTGAAGATC CGCATGCTGG ACGGCACCGT GAAGACTGTC
    ATGGTGGACG ACTCGAAGAC TGTCACGGAC ATGCTCATGA CCATCTGTGC CCGCATTGGC
    ATCACCAACC ATGATGAATA TTCACTGGTT CGAGAGATTA TGGAAGAGAA GAAGGAGGAG
    GTGACAGGGA CCTTACGAAA GGACAAGACA CTGCTGCGAG ACGAAAAGAA GATGGAGAAA
    CTGAAGCAGA AGTTGCACAC AGATGACGAG TTGAACTGGC TGGACCATGG CCGGACCCTG
    AGGGAGCAGG GAGTGGAGGA GCACGAAACG CTTCTGCTCC GGAGGAAGTT CTTCTACTCA
    GATCAGAACG TGGACTCCCG GGACCCCGTC CAGCTGAACC TTCTCTACGT GCAGGCACGA
    GATGACATCC TGAATGGCTC CCATCCTGTC TCCTTCGACA AGGCCTGTGA GTTTGCTGGC
    TTCCAATGCC AGATCCAGTT TGGGCCCCAC AATGAGCAGA AGCACAAGGC CGGCTTCCTC
    GACCTGAAGG ACTTCCTGCC CAAGGAGTAT GTGAAGCAGA AGGGAGAGCG TAAGATCTTC
    CAGGCACACA AGAACTGTGG ACAGATGAGT GAGATTGAGG CCAAGGTACG CTACGTGAAG
    CTAGCCCGTT CCCTCAAGAC GTACGGTGTC TCCTTCTTCC TGGTCAAGGA AAAGATGAAA
    GGAAAGAACA AGTTGGTGCC CAGGCTTCTG GGCATCACTA AGGAGTGTGT GATGCGAGTG
    GACGAGAAGA CCAAGGAGGT GATCCAGGAA TGGAACCTCA CCAACATAAA ACGCTGGGCT
    GCGTCTCCCA AGAGCTTCAC CCTGGATTTC GGAGACTACC AAGATGGCTA CTACTCGGTG
    CAGACGACCG AGGGGGAGCA GATCGCTCAG CTCATCGCTG GCTACATCGA CATCATCCTT
    AAGAAGAAAA AAAGCAAGGA TCATTTTGGG CTGGAGGGAG ACGAGGAGTC TACTATGCTG
    GAGGACTCCG TGTCCCCCAA AAAGTCCACT GTGCTTCAGC AGCAGTATAA CCGGGTGGGG
    AAGGTGGAGC ATGGCTCCGT GGCCCTGCCC GCCATCATGC GCTCCGGAGC CTCGGGTCCC
    GAGAATTTCC AGGTGGGCAG CATGCCACCT GCCCAGCAGC AGATCACCAG TGGCCAGATG
    CACCGAGGAC ACATGCCTCC TTTGACGTCA GCCCAGCAGG CGCTCACTGG GACCATCAAC
    TCCAGCATGC AGGCTGTGCA AGCTGCCCAG GCCACACTGG ATGACTTTGA CACTCTGCCC
    CCTCTGGGCC AGGACGCTGC CTCAAAGGCC TGGCGTAAGA ACAAGATGGA TGAATCCAAG
    CATGAAATCC ACTCCCAGGT AGACGCCATC ACCGCTGGTA CCGCCTCTGT GGTGAACCTC
    ACAGCAGGGG ACCCTGCTGA GACAGACTAC ACGGCAGTGG GCTGTGCGGT CACCACCATC
    TCCTCCAACC TGACGGAGAT GTCCCGTGGC GTGAAGCTGC TTGCTGCCCT GCTGGAGGAT
    GAAGGCGGCA GCGGCCGGCC CCTGCTGCAG GCGGCCAAGG GCCTGGCGGG GGCGGTGTCA
    GAGCTGCTGC GCAGTGCCCA GCCAGCCAGT GCCGAGCCCC GTCAGAACCT GCTGCAAGCA
    GCTGGGAACG TGGGCCAGGC CAGTGGGGAG CTGTTGCAAC AAATTGGGGA AAGTGATACT
    GACCCCCACT TCCAGATCTG TGCACCCAGC GGGGCTGGGT TTCCATCTCC CCCCGATCCC
    CCCACGGACG TGCTCATGCA GCTAGCCAAG GCCGTGGCAA GCGCTGCAGC TGCCCTGGTC
    CTCAAGGCCA AGAGTGTGGC CCAGCGAACA GAGGACTCAG GGCTTCAGAC CCAGGTCATC
    GCTGCGGCAA CACAGTGTGC CCTGTCCACC TCCCAGCTGG TGGCCTGTAC CAAGGTGGTG
    GCTCCTACTA TCAGCTCCCC AGTCTGCCAA GAACAGCTGG TAGAGGCTGG GCGACTAGTG
    GCCAAGGCTG TGGAGGGCTG TGTGTCCGCT TCCCAGGCCG CCACAGAGGA TGCCCAGCTG
    TTACGGGGGG TAGGTGCTGC GGCCACAGCT GTCACCCAGG CCCTGAATGA GCTGCTGCAG
    CACGTGAGGG CCCATGCCAC AGGGGCTGGG CCTGCTGGCC GCTACGACCA GGCCACTGAC
    ACCATCCTCA CTGTCACCTA TTTCATCTTC AGCTCCATGG GTGATGCTGG TGAGATGGTG
    CGACAGGCCC GCATCCTCGC CCAAGCCACC TCTGACCTGG TGAACGCCAT CAAGGCTGAT
    GCCGAAGGGG AGAGTGATCT GGAGAATTCC CGCAAGCTGT TAAGTGCCGC CAAGATCCTG
    GCCGATGCCA CAGCCAAGAT GGTGGAGGCT GCCAAGGGAG CAGCCGCCAA TCCTGACAGT
    GAGGAGCAAC AGCAGCGGCT GCGGGAGGCA GCAGAGGGTC TCCGCATGGC AACCAATGCC
    GCTGCACAGA ACGCCATCAA GAAAAAGCTG GTGCAGCGCC TTGAGCATGC AGCCAAACAG
    GCTGCAGCCT CGGCTACACA GACTATCGCA GCAGCCCAAC ATGCAACCTC CACTCCCAAG
    GCCTCTGCTG GCCCCCAGCC CCTGCTGGTG CAGAGCTGCA AGGCTGTGGC AGAGCAGATT
    CCACTGCTGG TGCAGGGCGT CCGAGGGAGC CAAGCGCAGC CTGACAGCCC CAGTGCTCAG
    CTAGCCCTCA TCGCTGCCAG CCAAAGCTTC CTGCAGCCAG GTGGGAAGAT GGTGGCAGCC
    GCAAAGGCCT CTGTGCCAAC AATTCAGGAC CAAGCATCGG CCATGCAGCT GAGTCAGTGT
    GCTAAAAACC TTGGCACCGC GTTGGCTGAA CTCCGTACTG CTGCCCAGAA GGCTCAGGAA
    GCGTGTGGGC CTTTGGAAAT GGATTCTGCT CTGAGTGTGG TGCAGAATCT GGAGAGAGAC
    CTGCAGGAAG TGAAGGCAGC TGCTCGAGAC GGCAAGCTGA AGCCCTTGCC TGGGGAGACG
    ATGGAGAAAT GTGCCCAGGA CCTGGGCAAC AGCACCAAAG CCGTGAGCTC CGCTATTGCC
    CAGCTGCTGG GGGAGGTCGC CCAGGGCAAT GAGAATTACG CAGGCATTGC AGCTCGGGAT
    GTGGCAGGTG GGCTGCGGTC ACTGGCCCAA GCTGCTAGGG GCGTAGCTGC CCTGACATCA
    GATCCTGCAG TGCAAGCCAT TGTGCTCGAC ACAGCCAGCG ATGTCCTGGA CAAGGCCAGC
    AGCCTCATCG AGGAGGCAAA GAAAGCAGCC GGCCATCCAG GGGACCCTGA GAGCCAGCAG
    CGGCTTGCCC AGGTGGCTAA AGCTGTGACC CAGGCCCTGA ACCGTTGCGT CAGCTGCCTG
    CCTGGCCAGC GTGATGTGGA CAATGCCCTG AGGGCAGTGG GAGATGCCAG CAAGCGACTC
    CTGAGTGACT CGCTTCCCCC CAGCACTGGG ACCTTTCAGG AGGCTCAGAG CCGGCTGAAT
    GAAGCCGCTG CCGGGCTGAA TCAGGCAGCC ACAGAGCTGG TGCAGGCCTC TCGGGGAACC
    CCTCAGGACC TGGCCCGAGC CTCAGGTCGA TTTGGACAAG ACTTCAGCAC CTTCCTGGAA
    GCCGGCGTGG AGATGGCAGG CCAGGCTCCG AGCCAGGAGG ACCGTGCCCA GGTCGTATCC
    AACTTGAAGG GCATCTCCAT GTCTTCAAGC AAACTTCTTC TCGCTGCCAA GGCCCTGTCC
    ACAGACCCTG CAGCCCCAAC CCTCAAGAGT CAGCTGGCTG CAGCTGCCCG GGCAGTGACC
    GACAGCATCA ACCAGCTCAT CAGCATGTGC ACCCAGCAGG CGCCAGGCCA GAAGGAGTGT
    GATAATGCCC TGCGGGAATT GGAGACGGTC CGGGAACTCC TAGAGAACCC AGTCCAGCCC
    ATCAATGACA TGTCCTACTT TGGCTGTCTG GACAGCGTCA TGGAAAACTC AAAGGTGCTG
    GGTGAGGCCA TGACCGGCAT CTCCCAAAAT GCCAAGAACG GAAACTTGCC GGAGTTTGGG
    GAGGCTGTCG CCACAGCCTC CAAGGCCCTC TGCGGCTTCA CCGAGGCAGC TGCACAGGCT
    GCATATCTGG TTGGCGTGTC TGACCCCAAC AGCCAAGCTG GACAACAAGG GCTGGTGGAG
    CCCACACAGT TTGCCCGAGC AAACCAGGCA ATTCAGATGG CCTGTCAGAG TTTGGGGGAG
    CCTGGCTGCA CCCAGGCCCA GGTGCTCTCT GCAGCCACCA TTGTGGCCAA ACACACGTCT
    GCGCTGTGTA ACAGCTGCCG CCTGGCTTCT GCCCGAACCG CCAATCCCAC TGCCAAGCGC
    CAGTTCGTAC AGTCAGCCAA GGAGGTGGCC AACAGCACGG CCAACCTTGT CAAGACCATC
    AAGGCGCTGG ATGGGGCCTT CACAGAGGAG AACCGTGCCC AGTGCCGAGC AGCAACGGCC
    CCTTTGCTGG AGGCTGTGGA CAATCTGAGC GCCTTTGCGT CCAACCCTGA GTTCTCCAGT
    GTTCCTGCCC AGATCAGCCC TGAGGGCCGG GCTGCCATGG AGCCCATTGT GATCTCTGCT
    AAGACAATGT TGGAGAGTGC TGGGGGCCTT ATTCAGACAG CCCGAGCCCT GGCGGTCAAT
    CCGCGGGACC CACCACGCTG GTCCGTGCTG GCTGGCCACT CCCGTACTGT CTCAGACTCC
    ATCAAGAAGC TGATCACAAG CATGAGGGAC AAGGCCCCAG GGCAGTTGGA GTGTGAGACA
    GCCATCGCAG CTCTGAACAG CTGTCTGCGG GACCTTGACC AGGCTTCTCT TGCTGCAGTC
    AGCCAGCAGC TCGCTCCCCG TGAGGGGATC TCTCAGGAGG CCTTGCACAC CCAGATGCTC
    ACTGCGGTGC AGGAAATCTC CCATCTTATT GAGCCGCTGG CCAGCGCTGC CCGGGCTGAA
    GCCTCCCAGC TGGGACACAA GGTGTCCCAG ATGGCCCAGT ACTTTGAGCC GCTCACTCTG
    GCTGCCGTGG GTGCTGCCTC CAAAACCCTG AGCCACCCAC AGCAGATGGC GCTCCTGGAC
    CAGACCAAAA CCTTGGCCGA GTCTGCCCTG CAGCTGCTAT ACACAGCCAA GGAAGCTGGC
    GGTAACCCCA AGCAAGCAGC TCACACCCAG GAAGCCCTGG AGGAGGCTGT GCAGATGATG
    ACAGAGGCCG TAGAGGACCT GACAACCACC CTCAACGAGG CAGCCAGTGC TGCCGGGGTC
    GTCGGTGGCA TGGTGGACTC CATCACCCAG GCCATCAACC AGCTAGATGA AGGACCAATG
    GGTGAACCAG AGGGTTCCTT CGTGGATTAC CAGACCACCA TGGTGCGGAC AGCCAAGGCC
    ATTGCTGTCA CCGTTCAGGA GATGGTAACC AAGTCAAACA CCAGCCCTGA GGAGCTGGGC
    CCTCTCGCTA ACCAGCTGAC TAGTGACTAT GGGCGTCTTG CCTCCCAGGC CAAGCCTGCA
    GCTGTGGCTG CTGAAAATGA AGAGATAGGC GCCCACATCA AGCACCGGGT TCAGGAGCTG
    GGGCACGGCT GCGCAGCTCT GGTCACCAAG GCAGGCGCCC TGCAGTGTAG CCCTAGTGAT
    GCCTACACCA AGAAGGAGCT CATAGAGTGT GCCCGGAGAG TCTCTGAGAA GGTCTCCCAC
    GTGCTGGCTG CGCTCCAGGC TGGGAATCGT GGCACCCAGG CCTGCATCAC AGCCGCCAGC
    GCTGTGTCCG GCATCATCGC CGACCTGGAC ACCACCATCA TGTTCGCCAC TGCCGGCACA
    CTCAACCGTG AGGGTGCCGA AACCTTTGCT GACCACCGAG AGGGCATTCT GAAGACTGCC
    AAGGTGCTGG TGGAAGACAC CAAGGTCCTG GTGCAGAATG CAGCTGGGAG CCAGGAGAAG
    CTGGCACAGG CAGCCCAGTC CTCCGTGGCC ACCATCACCC GCCTCGCCGA TGTGGTCAAG
    CTGGGTGCAG CCAGCCTGGG AGCTGAGGAC CCAGAGACCC AGGTGGTGCT GATCAATGCA
    GTGAAAGATG TGGCCAAGGC CCTGGGAGAC CTCATCAGTG CCACAAAGGC TGCGGCTGGC
    AAAGTCGGCG ACGACCCTGC TGTGTGGCAG CTCAAGAACT CTGCCAAGGT GATGGTAACC
    AATGTGACAT CACTGCTCAA GACCGTGAAA GCGGTAGAGG ATGAGGCCAC CAAAGGCACG
    CGCGCCCTGG AGGCCACAAC GGAGCATATT CGGCAGGAGC TGGCGGTCTT CTGCTCCCCA
    GAGCCACCTG CCAAGACCTC GACCCCTGAA GATTTCATCC GCATGACCAA GGGTATCACC
    ATGGCAACCG CCAAGGCCGT TGCCGCTGGG AACTCCTGTC GCCAGGAGGA TGTCATTGCC
    ACAGCCAATC TGAGTCGCCG TGCTATTGCA GATATGCTCC GGGCTTGCAA GGAAGCTGCC
    TACCACCCAG AAGTGGCTCC AGACGTGCGG CTCCGAGCCC TACACTTTGG CCGGGAATGT
    GCCAATGGCT ACCTGGAACT GCTGGACCAC GTGCTGCTGA CCCTGCAGAA GCCAAGCCCA
    GAATTGAAGC AGCAATTGAC GGTGCACTCA AAGCGTGTGG CTGGATCAGT CACTGAGCTC
    ATCCAGGCTG CTGAGGCCAT GAAGGGAACA GAATGGGTGG ACCCAGAGGA CCCCACAGTC
    ATTGCTGAGA ATGAACTCCT GGGAGCCGCG GCTGCCATTG AGGCTGCGGC CAAGAAGCTG
    GAGCAGCTGA AGCCCCGGGC CAAACCTAAG GAGGCGGACG AGTCCTTGAA CTTTGAGGAG
    CAGATACTGG AAGCTGCCAA GTCCATCGCA GCAGCCACCA GCGCCCTGGT GAAGGCTGCC
    TCAGCTGCAC AGAGGGAACT GGTGGCCCAG GGGAAGGTGG GCGCCATTCC AGCCAACGCA
    CTGGATGATG GACAGTGGTC CCAGGGCCTC ATCTCTGCTG CCCGGATGGT GGCTGCGGCC
    ACCAACAACC TGTGTGAGGC AGCCAATGCA GCTGTCCAAG GCCACGCCAG CCAGGAGAAG
    CTCATCTCAT CAGCCAAGCA GGTCGCCGCC TCCACAGCCC AGCTCCTCGT AGCCTGCAAG
    GTCAAGGCCG ACCAGGACTC TGAGGCAATG AAGCGGCTTC AGGCTGCCGG CAACGCAGTG
    AAGCGAGCCT CAGACAACCT GGTGAAGGCA GCCCAGAAGG CTGCAGCCTT TGAAGAACAG
    GAGAATGAGA CCGTGGTGGT GAAGGAGAAG ATGGTCGGGG GCATTGCCCA GATCATTGCT
    GCCCAGGAAG AGATGCTTCG GAAGGAACGA GAGCTGGAGG AGGCACGGAA GAAGCTGGCG
    CAGATCCGGC AGCAGCAGTA CAAATTCTTA CCTTCAGAGC TTCGAGACGA GCACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.