NLRC5 cDNA ORF clone, Bos taurus(cattle)

The following NLRC5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLRC5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa225469 XM_024978952.1
Latest version!
Bos taurus NLR family CARD domain containing 5 (NLRC5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa225470 XM_024978953.1
Latest version!
Bos taurus NLR family CARD domain containing 5 (NLRC5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa93394 XM_015467341.1
Latest version!
Bos taurus NLR family, CARD domain containing 5 (NLRC5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa93394 XM_015475802.1
Latest version!
Bos taurus NLR family, CARD domain containing 5 (NLRC5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa09294 XM_005195224.3
Latest version!
Bos taurus NLR family, CARD domain containing 5 (NLRC5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa09294 XM_024978950.1
Latest version!
Bos taurus NLR family CARD domain containing 5 (NLRC5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa09294 XM_024978951.1
Latest version!
Bos taurus NLR family CARD domain containing 5 (NLRC5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa09294 XM_005218805.4
Latest version!
Bos taurus NLR family CARD domain containing 5 (NLRC5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa57241 XM_010823574.2
Latest version!
Bos taurus NLR family, CARD domain containing 5 (NLRC5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa57241 XM_010814628.2
Latest version!
Bos taurus NLR family, CARD domain containing 5 (NLRC5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
OBa09294 XM_005218805.3 Bos taurus NLR family, CARD domain containing 5 (NLRC5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa225469
    Clone ID Related Accession (Same CDS sequence) XM_024978952.1
    Accession Version XM_024978952.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein NLRC5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG ACCTCTCAGG GAACCAGCTC AAAGATAAAG GCTGTCAACT GATGGCAGAG
    GCTGCTCCCC AACTGCACAT CACGAAGAAG CTGGACCTCA GCGACAATGA GCTTTCTGTG
    GCCGGGGTGC TGGGAGTACT GAGTGTGGTG AACACGTACC AAACCCTGAC AGAGCTACAT
    ATCAGCCTGC TGCACAAAAC TGTGGTCTTC ACGTTTGCCC CAGAGCTGGA GGAGCAGGAG
    GGGATCCAGA AGAGGGCTAC GTTTCCTGAC AGCCTTATGT TCCAGATGTC CTCTGAGCCA
    TCTCTGCGCT CCCCGAGGAT CAGGCTGACG CACTGTGCCC TGCAAGCCCA GCGCCTGGAG
    CTGCTCTGCA AAGCGCTGGG AGGAAGTTGC CGCCTCAGTC ACCTTGACTT ATCAGGCAAT
    GCTCTGGGGG ATGAAGGTGT GGCCCTGCTG GCTCGGCTGC TCCCCGGACT GGGTTCTCTG
    CAGTCCTTGA ACCTCAGTGA GAACCATTTG TCCCTGGAGG GTGTGTGCAG TTTGACCCAG
    TGCTTCTCTA CTCTGCGGTG GCTTCTACAT TTGGAATTCA GCTCTGCAAG CCAACACGTC
    ATCCTGAGAG GCGACAAAAG AGGCAGGGAT CTGTTGGCTG CTGGATCTTT GCCAGAGTTC
    CGAGCTGGAG CCCGGTTGGG ATCTGGTCAG CGCCACGTCC CCAGGAGCTT CTGCCTCAAG
    GAATGTCAGC TGGAGCCCCT GAGCCTCTCC TACCTCTGCA AGTCTCTGGA GAAGTGCCCA
    GGGCCCCTGG AAGTCCAATT GTCCTGCGAG GTGCTGAGTG ACCAGAGCCT GGAGACCCTG
    CTGTACCACC TACCGCAGCT CCCCCAGCTA AGCCTGCTGC AACTGAGCCA GATGCAACTG
    CCTCCAGAGA GCCCCCTCCT GCTAGCTGAC CTCTTCAGCC TGTGCCCACA GGTTCAGAAG
    GTGGATCTCA GGTCCCTGCG TCATTTGACC CTGCATCTCA GGTCTAGTGA GGAGCAGGAA
    GACAGGTGCT GTTGGTTCAC TGACTGCGGC CTCCGCCAGG AGCACGTGGA GCCGCTGTGC
    GGGTTGCTGA GCAAGTGTGA AGACCTCCAC CAGCTGGATC TAAGCCACAA CAGTATGTCT
    CAGGAAAGCG TCTTGTGCCT GGTGGAGGCG CTCCCCTCCT GCCCACGTCT CCGGGAGGCC
    TCAGTGAACC TGGGCTCCAA GCAGAGCTTC TGGATTCACT TCTCCCGACA GGAAGAGACT
    GGGAAGACAC TGAGGCTGAG CGAGTGCCAC TTCAAGCCAG AGCACATGCC CAGACTGGCC
    GCCAGCCTGA GCCAGGCCCT GCAGCTGACG GAGCTCACGC TGACCTGGTG TTTCCCGGGC
    CTGGAGCAGC TGACTGTCCT CCTGGGTCTG CTAACGTGGC CGACGGGGGT GCTCACTCTC
    AGGGTGGAAG AGCCATGGGT GGGCAGAGTC GGGGTGCTCA CGCTGCTGGA AGTCTGTGCC
    CAGGCCTCAG GCAACATCAC TGGAATAAGC ATCTCCGAGA CCCAGAAACA GCTCTGTATC
    CAGCTGGAAT TTCCCCATCA GGAGAACCCA GAAGCCGTGG CCCTCAGGCT GGCTCACTGT
    GACCTGGGAA CCCAACAAAA CCTCCTCGCC AGGAAGTTGA TGGAGACATG TGCCCGGCTG
    CGGCAGCTCA GTTTGTCCCA GGTGAACCTC TGCGATGCCA GCTCTCTGCT GCTGCAAAGC
    CTCCTGCTGT CCCTCTCTGA GCTGAAGAAC TTCAGGCTGA CCTCCAGCTG TGTGAGCTCC
    AAGGGGCTAG CCCTCCTGAC ATCTGGTCTG AGCCATTGTC ACCTTCTGGA GGAGCTAGAC
    TTGTCTAACA ATCAACTTGG AGACGAGGGC ACCAAGGTGT TGTTGGGGGC TCTTGAGGGC
    AAGTGCAGGC TGAAGAGACT TGACCTCAGC CACCTGCCAC TGGGCGGCTC CACCCTGGCT
    GTGCTCACTC AAGGACTAGG CCACATGACA CTCCTGCAGA GCCTCCGTCT GAGCAAGAGC
    AACATCGATG ACGTCGGCTG CTGCCACCTT TCGAAGGCTC TCAGAGCTGC CACCAGCTTG
    GAGGAGCTGA GCTTGAGCCA CAACCAGATC GGAAACATCG GTGCCCAGGA CTTAGCTGCT
    GTCCTGCCGG GGCTCCCTGA GCTCAGGAAG ATAGACCTCT CAGCAAATGG CATCGGCCCA
    GCAGGCGGAG CGCGGTTGGC AGAATCTCTC ACCCTTTGCA GGCACCTCGA GGAGCTGATG
    CTTGGCTGCA ATGCCCTGGG AGACTGCACA GCCCTGGGTC TGGCCAAGGG GCTGCCCCAG
    CACCTGAGGG TCCTGCACCT GCCATCCAGC CATCTGAGCC CAGAGGGGGT GCTGAGTCTG
    GGCCAGGCCC TGGATGGATG CCCACACGTG GAAGAGATCA GCTTGGCAGA GAACAGCCTG
    GCTGGAGGGG TCCCGCATTT CCATCAGGGG TTCCCAGTGC TCCGGAAGCT TGACCTGGTG
    TCGTGTGAGA TTGACAACCA GACCGCCAAG CCCCTCGCCG CCAGCTTCGT GCTCTGTCTG
    GCCCTGGAAG AAATCACGCT GTCCTGGAAT CTGCTCGGGG ATGAGGCAGC TGCTGAGCTG
    GCCCGGGTCC TGCCGCAGAT GGGCCGGCTG AAGAGAATGG ACCTGGAGAA AAATCAGATC
    ACAGCTTATG GAGCCTGGCT CCTGGCTGAA GGGCTGGCAC AGGGATCTGG CATCCAAGTC
    ATTCGCCTCT GGAATAACCG TATTCCCCCG GACATGGCCC AGCATCTGCA GAACCAGGAG
    CCCAGGCTGG ACTTTGCTTT CTTTGACAAC CAGCCACAGG TCATCCCTCG GGGTACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834720.1
    CDS582..6101
    Translation

    Target ORF information:

    RefSeq Version XM_024978952.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family CARD domain containing 5 (NLRC5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978952.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG ACCTCTCAGG GAACCAGCTC AAAGATAAAG GCTGTCAACT GATGGCAGAG
    GCTGCTCCCC AACTGCACAT CACGAAGAAG CTGGACCTCA GCGACAATGA GCTTTCTGTG
    GCCGGGGTGC TGGGAGTACT GAGTGTGGTG AACACGTACC AAACCCTGAC AGAGCTACAT
    ATCAGCCTGC TGCACAAAAC TGTGGTCTTC ACGTTTGCCC CAGAGCTGGA GGAGCAGGAG
    GGGATCCAGA AGAGGGCTAC GTTTCCTGAC AGCCTTATGT TCCAGATGTC CTCTGAGCCA
    TCTCTGCGCT CCCCGAGGAT CAGGCTGACG CACTGTGCCC TGCAAGCCCA GCGCCTGGAG
    CTGCTCTGCA AAGCGCTGGG AGGAAGTTGC CGCCTCAGTC ACCTTGACTT ATCAGGCAAT
    GCTCTGGGGG ATGAAGGTGT GGCCCTGCTG GCTCGGCTGC TCCCCGGACT GGGTTCTCTG
    CAGTCCTTGA ACCTCAGTGA GAACCATTTG TCCCTGGAGG GTGTGTGCAG TTTGACCCAG
    TGCTTCTCTA CTCTGCGGTG GCTTCTACAT TTGGAATTCA GCTCTGCAAG CCAACACGTC
    ATCCTGAGAG GCGACAAAAG AGGCAGGGAT CTGTTGGCTG CTGGATCTTT GCCAGAGTTC
    CGAGCTGGAG CCCGGTTGGG ATCTGGTCAG CGCCACGTCC CCAGGAGCTT CTGCCTCAAG
    GAATGTCAGC TGGAGCCCCT GAGCCTCTCC TACCTCTGCA AGTCTCTGGA GAAGTGCCCA
    GGGCCCCTGG AAGTCCAATT GTCCTGCGAG GTGCTGAGTG ACCAGAGCCT GGAGACCCTG
    CTGTACCACC TACCGCAGCT CCCCCAGCTA AGCCTGCTGC AACTGAGCCA GATGCAACTG
    CCTCCAGAGA GCCCCCTCCT GCTAGCTGAC CTCTTCAGCC TGTGCCCACA GGTTCAGAAG
    GTGGATCTCA GGTCCCTGCG TCATTTGACC CTGCATCTCA GGTCTAGTGA GGAGCAGGAA
    GACAGGTGCT GTTGGTTCAC TGACTGCGGC CTCCGCCAGG AGCACGTGGA GCCGCTGTGC
    GGGTTGCTGA GCAAGTGTGA AGACCTCCAC CAGCTGGATC TAAGCCACAA CAGTATGTCT
    CAGGAAAGCG TCTTGTGCCT GGTGGAGGCG CTCCCCTCCT GCCCACGTCT CCGGGAGGCC
    TCAGTGAACC TGGGCTCCAA GCAGAGCTTC TGGATTCACT TCTCCCGACA GGAAGAGACT
    GGGAAGACAC TGAGGCTGAG CGAGTGCCAC TTCAAGCCAG AGCACATGCC CAGACTGGCC
    GCCAGCCTGA GCCAGGCCCT GCAGCTGACG GAGCTCACGC TGACCTGGTG TTTCCCGGGC
    CTGGAGCAGC TGACTGTCCT CCTGGGTCTG CTAACGTGGC CGACGGGGGT GCTCACTCTC
    AGGGTGGAAG AGCCATGGGT GGGCAGAGTC GGGGTGCTCA CGCTGCTGGA AGTCTGTGCC
    CAGGCCTCAG GCAACATCAC TGGAATAAGC ATCTCCGAGA CCCAGAAACA GCTCTGTATC
    CAGCTGGAAT TTCCCCATCA GGAGAACCCA GAAGCCGTGG CCCTCAGGCT GGCTCACTGT
    GACCTGGGAA CCCAACAAAA CCTCCTCGCC AGGAAGTTGA TGGAGACATG TGCCCGGCTG
    CGGCAGCTCA GTTTGTCCCA GGTGAACCTC TGCGATGCCA GCTCTCTGCT GCTGCAAAGC
    CTCCTGCTGT CCCTCTCTGA GCTGAAGAAC TTCAGGCTGA CCTCCAGCTG TGTGAGCTCC
    AAGGGGCTAG CCCTCCTGAC ATCTGGTCTG AGCCATTGTC ACCTTCTGGA GGAGCTAGAC
    TTGTCTAACA ATCAACTTGG AGACGAGGGC ACCAAGGTGT TGTTGGGGGC TCTTGAGGGC
    AAGTGCAGGC TGAAGAGACT TGACCTCAGC CACCTGCCAC TGGGCGGCTC CACCCTGGCT
    GTGCTCACTC AAGGACTAGG CCACATGACA CTCCTGCAGA GCCTCCGTCT GAGCAAGAGC
    AACATCGATG ACGTCGGCTG CTGCCACCTT TCGAAGGCTC TCAGAGCTGC CACCAGCTTG
    GAGGAGCTGA GCTTGAGCCA CAACCAGATC GGAAACATCG GTGCCCAGGA CTTAGCTGCT
    GTCCTGCCGG GGCTCCCTGA GCTCAGGAAG ATAGACCTCT CAGCAAATGG CATCGGCCCA
    GCAGGCGGAG CGCGGTTGGC AGAATCTCTC ACCCTTTGCA GGCACCTCGA GGAGCTGATG
    CTTGGCTGCA ATGCCCTGGG AGACTGCACA GCCCTGGGTC TGGCCAAGGG GCTGCCCCAG
    CACCTGAGGG TCCTGCACCT GCCATCCAGC CATCTGAGCC CAGAGGGGGT GCTGAGTCTG
    GGCCAGGCCC TGGATGGATG CCCACACGTG GAAGAGATCA GCTTGGCAGA GAACAGCCTG
    GCTGGAGGGG TCCCGCATTT CCATCAGGGG TTCCCAGTGC TCCGGAAGCT TGACCTGGTG
    TCGTGTGAGA TTGACAACCA GACCGCCAAG CCCCTCGCCG CCAGCTTCGT GCTCTGTCTG
    GCCCTGGAAG AAATCACGCT GTCCTGGAAT CTGCTCGGGG ATGAGGCAGC TGCTGAGCTG
    GCCCGGGTCC TGCCGCAGAT GGGCCGGCTG AAGAGAATGG ACCTGGAGAA AAATCAGATC
    ACAGCTTATG GAGCCTGGCT CCTGGCTGAA GGGCTGGCAC AGGGATCTGG CATCCAAGTC
    ATTCGCCTCT GGAATAACCG TATTCCCCCG GACATGGCCC AGCATCTGCA GAACCAGGAG
    CCCAGGCTGG ACTTTGCTTT CTTTGACAAC CAGCCACAGG TCATCCCTCG GGGTACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225470
    Clone ID Related Accession (Same CDS sequence) XM_024978953.1
    Accession Version XM_024978953.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein NLRC5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG ACCTCTCAGG GAACCAGCTC AAAGATAAAG GCTGTCAACT GATGGCAGAG
    GCTGCTCCCC AACTGCACAT CACGAAGAAG CTGGACCTCA GCGACAATGA GCTTTCTGTG
    GCCGGGGTGC TGGGAGTACT GAGTGTGGTG AACACGTACC AAACCCTGAC AGAGCTACAT
    ATCAGCCTGC TGCACAAAAC TGTGGTCTTC ACGTTTGCCC CAGAGCTGGA GGAGCAGGAG
    GGGATCCAGA AGAGGGCTAC GTTTCCTGAC AGCCTTATGT TCCAGATGTC CTCTGAGCCA
    TCTCTGCGCT CCCCGAGGAT CAGGCTGACG CACTGTGCCC TGCAAGCCCA GCGCCTGGAG
    CTGCTCTGCA AAGCGCTGGG AGGAAGTTGC CGCCTCAGTC ACCTTGACTT ATCAGGCAAT
    GCTCTGGGGG ATGAAGGTGT GGCCCTGCTG GCTCGGCTGC TCCCCGGACT GGGTTCTCTG
    CAGTCCTTGA ACCTCAGTGA GAACCATTTG TCCCTGGAGG GTGTGTGCAG TTTGACCCAG
    TGCTTCTCTA CTCTGCGGTG GCTTCTACAT TTGGAATTCA GCTCTGCAAG CCAACACGTC
    ATCCTGAGAG GCGACAAAAG AGGCAGGGAT CTGTTGGCTG CTGGATCTTT GCCAGAGTTC
    CGAGCTGGAG CCCGGTTGGG ATCTGGTCAG CGCCACGTCC CCAGGAGCTT CTGCCTCAAG
    GAATGTCAGC TGGAGCCCCT GAGCCTCTCC TACCTCTGCA AGTCTCTGGA GAAGTGCCCA
    GGGCCCCTGG AAGTCCAATT GTCCTGCGAG GTGCTGAGTG ACCAGAGCCT GGAGACCCTG
    CTGTACCACC TACCGCAGCT CCCCCAGCTA AGCCTGCTGC AACTGAGCCA GATGCAACTG
    CCTCCAGAGA GCCCCCTCCT GCTAGCTGAC CTCTTCAGCC TGTGCCCACA GGTTCAGAAG
    GTGGATCTCA GGTCCCTGCG TCATTTGACC CTGCATCTCA GGTCTAGTGA GGAGCAGGAA
    GACAGGTGCT GTTGGTTCAC TGACTGCGGC CTCCGCCAGG AGCACGTGGA GCCGCTGTGC
    GGGTTGCTGA GCAAGTGTGA AGACCTCCAC CAGCTGGACC TCTCAGCAAA CCTGCTGGGT
    GATGATGGGC TCAGGTGCCT CCTGGAATGT CTCCCCAAGG TGCCCATTTC CGGTTCGCTT
    GATCTAAGCC ACAACAGTAT GTCTCAGGAA AGCGTCTTGT GCCTGGTGGA GGCGCTCCCC
    TCCTGCCCAC GTCTCCGGGA GGCCTCAGTG AACCTGGGCT CCAAGCAGAG CTTCTGGATT
    CACTTCTCCC GACAGGAAGA GACTGGGAAG ACACTGAGGC TGAGCGAGTG CCACTTCAAG
    CCAGAGCACA TGCCCAGACT GGCCGCCAGC CTGAGCCAGG CCCTGCAGCT GACGGAGCTC
    ACGCTGACCT GGTGTTTCCC GGGCCTGGAG CAGCTGACTG TCCTCCTGGG TCTGCTAACG
    TGGCCGACGG GGGTGCTCAC TCTCAGGGTG GAAGAGCCAT GGGTGGGCAG AGTCGGGGTG
    CTCACGCTGC TGGAAGTCTG TGCCCAGGCC TCAGGCAACA TCACTGGAAT AAGCATCTCC
    GAGACCCAGA AACAGCTCTG TATCCAGCTG GAATTTCCCC ATCAGGAGAA CCCAGAAGCC
    GTGGCCCTCA GGCTGGCTCA CTGTGACCTG GGAACCCAAC AAAACCTCCT CGCCAGGAAG
    TTGATGGAGA CATGTGCCCG GCTGCGGCAG CTCAGTTTGT CCCAGGTGAA CCTCTGCGAT
    GCCAGCTCTC TGCTGCTGCA AAGCCTCCTG CTGTCCCTCT CTGAGCTGAA GAACTTCAGG
    CTGACCTCCA GCTGTGTGAG CTCCAAGGGG CTAGCCCTCC TGACATCTGG TCTGAGCCAT
    TGTCACCTTC TGGAGGAGCT AGACTTGTCT AACAATCAAC TTGGAGACGA GGGCACCAAG
    GTGTTGTTGG GGGCTCTTGA GGGCAAGTGC AGGCTGAAGA GACTTGACCT CAGCCACCTG
    CCACTGGGCG GCTCCACCCT GGCTGTGCTC ACTCAAGGAC TAGGCCACAT GACACTCCTG
    CAGAGCCTCC GTCTGAGCAA GAGCAACATC GATGACGTCG GCTGCTGCCA CCTTTCGAAG
    GCTCTCAGAG CTGCCACCAG CTTGGAGGAG CTGAGCCTCT CAGCAAATGG CATCGGCCCA
    GCAGGCGGAG CGCGGTTGGC AGAATCTCTC ACCCTTTGCA GGCACCTCGA GGAGCTGATG
    CTTGGCTGCA ATGCCCTGGG AGACTGCACA GCCCTGGGTC TGGCCAAGGG GCTGCCCCAG
    CACCTGAGGG TCCTGCACCT GCCATCCAGC CATCTGAGCC CAGAGGGGGT GCTGAGTCTG
    GGCCAGGCCC TGGATGGATG CCCACACGTG GAAGAGATCA GCTTGGCAGA GAACAGCCTG
    GCTGGAGGGG TCCCGCATTT CCATCAGGGG TTCCCAGTGC TCCGGAAGCT TGACCTGGTG
    TCGTGTGAGA TTGACAACCA GACCGCCAAG CCCCTCGCCG CCAGCTTCGT GCTCTGTCTG
    GCCCTGGAAG AAATCACGCT GTCCTGGAAT CTGCTCGGGG ATGAGGCAGC TGCTGAGCTG
    GCCCGGGTCC TGCCGCAGAT GGGCCGGCTG AAGAGAATGG ACCTGGAGAA AAATCAGATC
    ACAGCTTATG GAGCCTGGCT CCTGGCTGAA GGGCTGGCAC AGGGATCTGG CATCCAAGTC
    ATTCGCCTCT GGAATAACCG TATTCCCCCG GACATGGCCC AGCATCTGCA GAACCAGGAG
    CCCAGGCTGG ACTTTGCTTT CTTTGACAAC CAGCCACAGG TCATCCCTCG GGGTACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834721.1
    CDS582..6101
    Translation

    Target ORF information:

    RefSeq Version XM_024978953.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family CARD domain containing 5 (NLRC5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978953.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG ACCTCTCAGG GAACCAGCTC AAAGATAAAG GCTGTCAACT GATGGCAGAG
    GCTGCTCCCC AACTGCACAT CACGAAGAAG CTGGACCTCA GCGACAATGA GCTTTCTGTG
    GCCGGGGTGC TGGGAGTACT GAGTGTGGTG AACACGTACC AAACCCTGAC AGAGCTACAT
    ATCAGCCTGC TGCACAAAAC TGTGGTCTTC ACGTTTGCCC CAGAGCTGGA GGAGCAGGAG
    GGGATCCAGA AGAGGGCTAC GTTTCCTGAC AGCCTTATGT TCCAGATGTC CTCTGAGCCA
    TCTCTGCGCT CCCCGAGGAT CAGGCTGACG CACTGTGCCC TGCAAGCCCA GCGCCTGGAG
    CTGCTCTGCA AAGCGCTGGG AGGAAGTTGC CGCCTCAGTC ACCTTGACTT ATCAGGCAAT
    GCTCTGGGGG ATGAAGGTGT GGCCCTGCTG GCTCGGCTGC TCCCCGGACT GGGTTCTCTG
    CAGTCCTTGA ACCTCAGTGA GAACCATTTG TCCCTGGAGG GTGTGTGCAG TTTGACCCAG
    TGCTTCTCTA CTCTGCGGTG GCTTCTACAT TTGGAATTCA GCTCTGCAAG CCAACACGTC
    ATCCTGAGAG GCGACAAAAG AGGCAGGGAT CTGTTGGCTG CTGGATCTTT GCCAGAGTTC
    CGAGCTGGAG CCCGGTTGGG ATCTGGTCAG CGCCACGTCC CCAGGAGCTT CTGCCTCAAG
    GAATGTCAGC TGGAGCCCCT GAGCCTCTCC TACCTCTGCA AGTCTCTGGA GAAGTGCCCA
    GGGCCCCTGG AAGTCCAATT GTCCTGCGAG GTGCTGAGTG ACCAGAGCCT GGAGACCCTG
    CTGTACCACC TACCGCAGCT CCCCCAGCTA AGCCTGCTGC AACTGAGCCA GATGCAACTG
    CCTCCAGAGA GCCCCCTCCT GCTAGCTGAC CTCTTCAGCC TGTGCCCACA GGTTCAGAAG
    GTGGATCTCA GGTCCCTGCG TCATTTGACC CTGCATCTCA GGTCTAGTGA GGAGCAGGAA
    GACAGGTGCT GTTGGTTCAC TGACTGCGGC CTCCGCCAGG AGCACGTGGA GCCGCTGTGC
    GGGTTGCTGA GCAAGTGTGA AGACCTCCAC CAGCTGGACC TCTCAGCAAA CCTGCTGGGT
    GATGATGGGC TCAGGTGCCT CCTGGAATGT CTCCCCAAGG TGCCCATTTC CGGTTCGCTT
    GATCTAAGCC ACAACAGTAT GTCTCAGGAA AGCGTCTTGT GCCTGGTGGA GGCGCTCCCC
    TCCTGCCCAC GTCTCCGGGA GGCCTCAGTG AACCTGGGCT CCAAGCAGAG CTTCTGGATT
    CACTTCTCCC GACAGGAAGA GACTGGGAAG ACACTGAGGC TGAGCGAGTG CCACTTCAAG
    CCAGAGCACA TGCCCAGACT GGCCGCCAGC CTGAGCCAGG CCCTGCAGCT GACGGAGCTC
    ACGCTGACCT GGTGTTTCCC GGGCCTGGAG CAGCTGACTG TCCTCCTGGG TCTGCTAACG
    TGGCCGACGG GGGTGCTCAC TCTCAGGGTG GAAGAGCCAT GGGTGGGCAG AGTCGGGGTG
    CTCACGCTGC TGGAAGTCTG TGCCCAGGCC TCAGGCAACA TCACTGGAAT AAGCATCTCC
    GAGACCCAGA AACAGCTCTG TATCCAGCTG GAATTTCCCC ATCAGGAGAA CCCAGAAGCC
    GTGGCCCTCA GGCTGGCTCA CTGTGACCTG GGAACCCAAC AAAACCTCCT CGCCAGGAAG
    TTGATGGAGA CATGTGCCCG GCTGCGGCAG CTCAGTTTGT CCCAGGTGAA CCTCTGCGAT
    GCCAGCTCTC TGCTGCTGCA AAGCCTCCTG CTGTCCCTCT CTGAGCTGAA GAACTTCAGG
    CTGACCTCCA GCTGTGTGAG CTCCAAGGGG CTAGCCCTCC TGACATCTGG TCTGAGCCAT
    TGTCACCTTC TGGAGGAGCT AGACTTGTCT AACAATCAAC TTGGAGACGA GGGCACCAAG
    GTGTTGTTGG GGGCTCTTGA GGGCAAGTGC AGGCTGAAGA GACTTGACCT CAGCCACCTG
    CCACTGGGCG GCTCCACCCT GGCTGTGCTC ACTCAAGGAC TAGGCCACAT GACACTCCTG
    CAGAGCCTCC GTCTGAGCAA GAGCAACATC GATGACGTCG GCTGCTGCCA CCTTTCGAAG
    GCTCTCAGAG CTGCCACCAG CTTGGAGGAG CTGAGCCTCT CAGCAAATGG CATCGGCCCA
    GCAGGCGGAG CGCGGTTGGC AGAATCTCTC ACCCTTTGCA GGCACCTCGA GGAGCTGATG
    CTTGGCTGCA ATGCCCTGGG AGACTGCACA GCCCTGGGTC TGGCCAAGGG GCTGCCCCAG
    CACCTGAGGG TCCTGCACCT GCCATCCAGC CATCTGAGCC CAGAGGGGGT GCTGAGTCTG
    GGCCAGGCCC TGGATGGATG CCCACACGTG GAAGAGATCA GCTTGGCAGA GAACAGCCTG
    GCTGGAGGGG TCCCGCATTT CCATCAGGGG TTCCCAGTGC TCCGGAAGCT TGACCTGGTG
    TCGTGTGAGA TTGACAACCA GACCGCCAAG CCCCTCGCCG CCAGCTTCGT GCTCTGTCTG
    GCCCTGGAAG AAATCACGCT GTCCTGGAAT CTGCTCGGGG ATGAGGCAGC TGCTGAGCTG
    GCCCGGGTCC TGCCGCAGAT GGGCCGGCTG AAGAGAATGG ACCTGGAGAA AAATCAGATC
    ACAGCTTATG GAGCCTGGCT CCTGGCTGAA GGGCTGGCAC AGGGATCTGG CATCCAAGTC
    ATTCGCCTCT GGAATAACCG TATTCCCCCG GACATGGCCC AGCATCTGCA GAACCAGGAG
    CCCAGGCTGG ACTTTGCTTT CTTTGACAAC CAGCCACAGG TCATCCCTCG GGGTACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93394
    Clone ID Related Accession (Same CDS sequence) XM_015467341.1 , XM_015475802.1
    Accession Version XM_015467341.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein NLRC5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG AGCTGACGCA CTGTGCCCTG CAAGCCCAGC GCCTGGAGCT GCTCTGCAAA
    GCGCTGGGAG GAAGTTGCCG CCTCAGTCAC CTTGACTTAT CAGGCAATGC TCTGGGGGAT
    GAAGGTGTGG CCCTGCTGGC TCGGCTGCTC CCCGGACTGG GTTCTCTGCA GTCCTTGAAC
    CTCAGTGAGA ACCATTTGTC CCTGGAGGGT GTGTGCAGTT TGACCCAGTG CTTCTCTACT
    CTGCGGTGGC TTCTACATTT GGAATTCAGC TCTGCAAGCC AACACGTCAT CCTGAGAGGC
    GACAAAAGAG GCAGGGATCT GTTGGCTGCT GGATCTTTGC CAGAGTTCCG AGCTGGAGCC
    CGGTTGGGAT CTGGTCAGCG CCACGTCCCC AGGAGCTTCT GCCTCAAGGA ATGTCAGCTG
    GAGCCCCTGA GCCTCTCCTA CCTCTGCAAG TCTCTGGAGA AGTGCCCAGG GCCCCTGGAA
    GTCCAATTGT CCTGCGAGGT GCTGAGTGAC CAGAGCCTGG AGACCCTGCT GTACCACCTA
    CCGCAGCTCC CCCAGCTAAG CCTGCTGCAA CTGAGCCAGA TGCAACTGCC TCCAGAGAGC
    CCCCTCCTGC TAGCTGACCT CTTCAGCCTG TGCCCACAGG TTCAGAAGGT GGATCTCAGG
    TCCCTGCGTC ATTTGACCCT GCATCTCAGG TCTAGTGAGG AGCAGGAAGA CAGGTGCTGT
    TGGTTCACTG ACTGCGGCCT CCGCCAGGAG CACGTGGAGC CGCTGTGCGG GTTGCTGAGC
    AAGTGTGAAG ACCTCCACCA GCTGGACCTC TCAGCAAACC TGCTGGGTGA TGATGGGCTC
    AGGTGCCTCC TGGAATGTCT CCCCAAGGTG CCCATTTCCG GTTCGCTTGA TCTAAGCCAC
    AACAGTATGT CTCAGGAAAG CGTCTTGTGC CTGGTGGAGG CGCTCCCCTC CTGCCCACGT
    CTCCGGGAGG CCTCAGTGAA CCTGGGCTCC AAGCAGAGCT TCTGGATTCA CTTCTCCCGA
    CAGGAAGAGA CTGGGAAGAC ACTGAGGCTG AGCGAGTGCC ACTTCAAGCC AGAGCACATG
    CCCAGACTGG CCGCCAGCCT GAGCCAGGCC CTGCAGCTGA CGGAGCTCAC GCTGACCTGG
    TGTTTCCCGG GCCTGGAGCA GCTGACTGTC CTCCTGGGTC TGCTAACGTG GCCGACGGGG
    GTGCTCACTC TCAGGGTGGA AGAGCCATGG GTGGGCAGAG TCGGGGTGCT CACGCTGCTG
    GAAGTCTGTG CCCAGGCCTC AGGCAACATC ACTGGAATAA GCATCTCCGA GACCCAGAAA
    CAGCTCTGTA TCCAGCTGGA ATTTCCCCAT CAGGAGAACC CAGAAGCCGT GGCCCTCAGG
    CTGGCTCACT GTGACCTGGG AACCCAACAA AACCTCCTCG CCAGGAAGTT GATGGAGACA
    TGTGCCCGGC TGCGGCAGCT CAGTTTGTCC CAGGTGAACC TCTGCGATGC CAGCTCTCTG
    CTGCTGCAAA GCCTCCTGCT GTCCCTCTCT GAGCTGAAGA ACTTCAGGCT GACCTCCAGC
    TGTGTGAGCT CCAAGGGGCT AGCCCTCCTG ACATCTGGTC TGAGCCATTG TCACCTTCTG
    GAGGAGCTAG ACTTGTCTAA CAATCAACTT GGAGACGAGG GCACCAAGGT GTTGTTGGGG
    GCTCTTGAGG GCAAGTGCAG GCTGAAGAGA CTTGACCTCA GCCACCTGCC ACTGGGCGGC
    TCCACCCTGG CTGTGCTCAC TCAAGGACTA GGCCACATGA CACTCCTGCA GAGCCTCCGT
    CTGAGCAAGA GCAACATCGA TGACGTCGGC TGCTGCCACC TTTCGAAGGC TCTCAGAGCT
    GCCACCAGCT TGGAGGAGCT GAGCTTGAGC CACAACCAGA TCGGAAACAT CGGTGCCCAG
    GACTTAGCTG CTGTCCTGCC GGGGCTCCCT GAGCTCAGGA AGATAGACCT CTCAGCAAAT
    GGCATCGGCC CAGCAGGCGG AGCGCGGTTG GCAGAATCTC TCACCCTTTG CAGGCACCTC
    GAGGAGCTGA TGCTTGGCTG CAATGCCCTG GGAGACTGCA CAGCCCTGGG TCTGGCCAAG
    GGGCTGCCCC AGCACCTGAG GGTCCTGCAC CTGCCATCCA GCCATCTGAG CCCAGAGGGG
    GTGCTGAGTC TGGGCCAGGC CCTGGATGGA TGCCCACACG TGGAAGAGAT CAGCTTGGCA
    GAGAACAGCC TGGCTGGAGG GGTCCCGCAT TTCCATCAGG GGTTCCCAGT GCTCCGGAAG
    CTTGACCTGG TGTCGTGTGA GATTGACAAC CAGACCGCCA AGCCCCTCGC CGCCAGCTTC
    GTGCTCTGTC TGGCCCTGGA AGAAATCACG CTGTCCTGGA ATCTGCTCGG GGATGAGGCA
    GCTGCTGAGC TGGCCCGGGT CCTGCCGCAG ATGGGCCGGC TGAAGAGAAT GGACCTGGAG
    AAAAATCAGA TCACAGCTTA TGGAGCCTGG CTCCTGGCTG AAGGGCTGGC ACAGGGATCT
    GGCATCCAAG TCATTCGCCT CTGGAATAAC CGTATTCCCC CGGACATGGC CCAGCATCTG
    CAGAACCAGG AGCCCAGGCT GGACTTTGCT TTCTTTGACA ACCAGCCACA GGTCATCCCT
    CGGGGTACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322827.1
    CDS552..5843
    Translation

    Target ORF information:

    RefSeq Version XM_015467341.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, CARD domain containing 5 (NLRC5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467341.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG AGCTGACGCA CTGTGCCCTG CAAGCCCAGC GCCTGGAGCT GCTCTGCAAA
    GCGCTGGGAG GAAGTTGCCG CCTCAGTCAC CTTGACTTAT CAGGCAATGC TCTGGGGGAT
    GAAGGTGTGG CCCTGCTGGC TCGGCTGCTC CCCGGACTGG GTTCTCTGCA GTCCTTGAAC
    CTCAGTGAGA ACCATTTGTC CCTGGAGGGT GTGTGCAGTT TGACCCAGTG CTTCTCTACT
    CTGCGGTGGC TTCTACATTT GGAATTCAGC TCTGCAAGCC AACACGTCAT CCTGAGAGGC
    GACAAAAGAG GCAGGGATCT GTTGGCTGCT GGATCTTTGC CAGAGTTCCG AGCTGGAGCC
    CGGTTGGGAT CTGGTCAGCG CCACGTCCCC AGGAGCTTCT GCCTCAAGGA ATGTCAGCTG
    GAGCCCCTGA GCCTCTCCTA CCTCTGCAAG TCTCTGGAGA AGTGCCCAGG GCCCCTGGAA
    GTCCAATTGT CCTGCGAGGT GCTGAGTGAC CAGAGCCTGG AGACCCTGCT GTACCACCTA
    CCGCAGCTCC CCCAGCTAAG CCTGCTGCAA CTGAGCCAGA TGCAACTGCC TCCAGAGAGC
    CCCCTCCTGC TAGCTGACCT CTTCAGCCTG TGCCCACAGG TTCAGAAGGT GGATCTCAGG
    TCCCTGCGTC ATTTGACCCT GCATCTCAGG TCTAGTGAGG AGCAGGAAGA CAGGTGCTGT
    TGGTTCACTG ACTGCGGCCT CCGCCAGGAG CACGTGGAGC CGCTGTGCGG GTTGCTGAGC
    AAGTGTGAAG ACCTCCACCA GCTGGACCTC TCAGCAAACC TGCTGGGTGA TGATGGGCTC
    AGGTGCCTCC TGGAATGTCT CCCCAAGGTG CCCATTTCCG GTTCGCTTGA TCTAAGCCAC
    AACAGTATGT CTCAGGAAAG CGTCTTGTGC CTGGTGGAGG CGCTCCCCTC CTGCCCACGT
    CTCCGGGAGG CCTCAGTGAA CCTGGGCTCC AAGCAGAGCT TCTGGATTCA CTTCTCCCGA
    CAGGAAGAGA CTGGGAAGAC ACTGAGGCTG AGCGAGTGCC ACTTCAAGCC AGAGCACATG
    CCCAGACTGG CCGCCAGCCT GAGCCAGGCC CTGCAGCTGA CGGAGCTCAC GCTGACCTGG
    TGTTTCCCGG GCCTGGAGCA GCTGACTGTC CTCCTGGGTC TGCTAACGTG GCCGACGGGG
    GTGCTCACTC TCAGGGTGGA AGAGCCATGG GTGGGCAGAG TCGGGGTGCT CACGCTGCTG
    GAAGTCTGTG CCCAGGCCTC AGGCAACATC ACTGGAATAA GCATCTCCGA GACCCAGAAA
    CAGCTCTGTA TCCAGCTGGA ATTTCCCCAT CAGGAGAACC CAGAAGCCGT GGCCCTCAGG
    CTGGCTCACT GTGACCTGGG AACCCAACAA AACCTCCTCG CCAGGAAGTT GATGGAGACA
    TGTGCCCGGC TGCGGCAGCT CAGTTTGTCC CAGGTGAACC TCTGCGATGC CAGCTCTCTG
    CTGCTGCAAA GCCTCCTGCT GTCCCTCTCT GAGCTGAAGA ACTTCAGGCT GACCTCCAGC
    TGTGTGAGCT CCAAGGGGCT AGCCCTCCTG ACATCTGGTC TGAGCCATTG TCACCTTCTG
    GAGGAGCTAG ACTTGTCTAA CAATCAACTT GGAGACGAGG GCACCAAGGT GTTGTTGGGG
    GCTCTTGAGG GCAAGTGCAG GCTGAAGAGA CTTGACCTCA GCCACCTGCC ACTGGGCGGC
    TCCACCCTGG CTGTGCTCAC TCAAGGACTA GGCCACATGA CACTCCTGCA GAGCCTCCGT
    CTGAGCAAGA GCAACATCGA TGACGTCGGC TGCTGCCACC TTTCGAAGGC TCTCAGAGCT
    GCCACCAGCT TGGAGGAGCT GAGCTTGAGC CACAACCAGA TCGGAAACAT CGGTGCCCAG
    GACTTAGCTG CTGTCCTGCC GGGGCTCCCT GAGCTCAGGA AGATAGACCT CTCAGCAAAT
    GGCATCGGCC CAGCAGGCGG AGCGCGGTTG GCAGAATCTC TCACCCTTTG CAGGCACCTC
    GAGGAGCTGA TGCTTGGCTG CAATGCCCTG GGAGACTGCA CAGCCCTGGG TCTGGCCAAG
    GGGCTGCCCC AGCACCTGAG GGTCCTGCAC CTGCCATCCA GCCATCTGAG CCCAGAGGGG
    GTGCTGAGTC TGGGCCAGGC CCTGGATGGA TGCCCACACG TGGAAGAGAT CAGCTTGGCA
    GAGAACAGCC TGGCTGGAGG GGTCCCGCAT TTCCATCAGG GGTTCCCAGT GCTCCGGAAG
    CTTGACCTGG TGTCGTGTGA GATTGACAAC CAGACCGCCA AGCCCCTCGC CGCCAGCTTC
    GTGCTCTGTC TGGCCCTGGA AGAAATCACG CTGTCCTGGA ATCTGCTCGG GGATGAGGCA
    GCTGCTGAGC TGGCCCGGGT CCTGCCGCAG ATGGGCCGGC TGAAGAGAAT GGACCTGGAG
    AAAAATCAGA TCACAGCTTA TGGAGCCTGG CTCCTGGCTG AAGGGCTGGC ACAGGGATCT
    GGCATCCAAG TCATTCGCCT CTGGAATAAC CGTATTCCCC CGGACATGGC CCAGCATCTG
    CAGAACCAGG AGCCCAGGCT GGACTTTGCT TTCTTTGACA ACCAGCCACA GGTCATCCCT
    CGGGGTACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93394
    Clone ID Related Accession (Same CDS sequence) XM_015467341.1 , XM_015475802.1
    Accession Version XM_015475802.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein NLRC5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG AGCTGACGCA CTGTGCCCTG CAAGCCCAGC GCCTGGAGCT GCTCTGCAAA
    GCGCTGGGAG GAAGTTGCCG CCTCAGTCAC CTTGACTTAT CAGGCAATGC TCTGGGGGAT
    GAAGGTGTGG CCCTGCTGGC TCGGCTGCTC CCCGGACTGG GTTCTCTGCA GTCCTTGAAC
    CTCAGTGAGA ACCATTTGTC CCTGGAGGGT GTGTGCAGTT TGACCCAGTG CTTCTCTACT
    CTGCGGTGGC TTCTACATTT GGAATTCAGC TCTGCAAGCC AACACGTCAT CCTGAGAGGC
    GACAAAAGAG GCAGGGATCT GTTGGCTGCT GGATCTTTGC CAGAGTTCCG AGCTGGAGCC
    CGGTTGGGAT CTGGTCAGCG CCACGTCCCC AGGAGCTTCT GCCTCAAGGA ATGTCAGCTG
    GAGCCCCTGA GCCTCTCCTA CCTCTGCAAG TCTCTGGAGA AGTGCCCAGG GCCCCTGGAA
    GTCCAATTGT CCTGCGAGGT GCTGAGTGAC CAGAGCCTGG AGACCCTGCT GTACCACCTA
    CCGCAGCTCC CCCAGCTAAG CCTGCTGCAA CTGAGCCAGA TGCAACTGCC TCCAGAGAGC
    CCCCTCCTGC TAGCTGACCT CTTCAGCCTG TGCCCACAGG TTCAGAAGGT GGATCTCAGG
    TCCCTGCGTC ATTTGACCCT GCATCTCAGG TCTAGTGAGG AGCAGGAAGA CAGGTGCTGT
    TGGTTCACTG ACTGCGGCCT CCGCCAGGAG CACGTGGAGC CGCTGTGCGG GTTGCTGAGC
    AAGTGTGAAG ACCTCCACCA GCTGGACCTC TCAGCAAACC TGCTGGGTGA TGATGGGCTC
    AGGTGCCTCC TGGAATGTCT CCCCAAGGTG CCCATTTCCG GTTCGCTTGA TCTAAGCCAC
    AACAGTATGT CTCAGGAAAG CGTCTTGTGC CTGGTGGAGG CGCTCCCCTC CTGCCCACGT
    CTCCGGGAGG CCTCAGTGAA CCTGGGCTCC AAGCAGAGCT TCTGGATTCA CTTCTCCCGA
    CAGGAAGAGA CTGGGAAGAC ACTGAGGCTG AGCGAGTGCC ACTTCAAGCC AGAGCACATG
    CCCAGACTGG CCGCCAGCCT GAGCCAGGCC CTGCAGCTGA CGGAGCTCAC GCTGACCTGG
    TGTTTCCCGG GCCTGGAGCA GCTGACTGTC CTCCTGGGTC TGCTAACGTG GCCGACGGGG
    GTGCTCACTC TCAGGGTGGA AGAGCCATGG GTGGGCAGAG TCGGGGTGCT CACGCTGCTG
    GAAGTCTGTG CCCAGGCCTC AGGCAACATC ACTGGAATAA GCATCTCCGA GACCCAGAAA
    CAGCTCTGTA TCCAGCTGGA ATTTCCCCAT CAGGAGAACC CAGAAGCCGT GGCCCTCAGG
    CTGGCTCACT GTGACCTGGG AACCCAACAA AACCTCCTCG CCAGGAAGTT GATGGAGACA
    TGTGCCCGGC TGCGGCAGCT CAGTTTGTCC CAGGTGAACC TCTGCGATGC CAGCTCTCTG
    CTGCTGCAAA GCCTCCTGCT GTCCCTCTCT GAGCTGAAGA ACTTCAGGCT GACCTCCAGC
    TGTGTGAGCT CCAAGGGGCT AGCCCTCCTG ACATCTGGTC TGAGCCATTG TCACCTTCTG
    GAGGAGCTAG ACTTGTCTAA CAATCAACTT GGAGACGAGG GCACCAAGGT GTTGTTGGGG
    GCTCTTGAGG GCAAGTGCAG GCTGAAGAGA CTTGACCTCA GCCACCTGCC ACTGGGCGGC
    TCCACCCTGG CTGTGCTCAC TCAAGGACTA GGCCACATGA CACTCCTGCA GAGCCTCCGT
    CTGAGCAAGA GCAACATCGA TGACGTCGGC TGCTGCCACC TTTCGAAGGC TCTCAGAGCT
    GCCACCAGCT TGGAGGAGCT GAGCTTGAGC CACAACCAGA TCGGAAACAT CGGTGCCCAG
    GACTTAGCTG CTGTCCTGCC GGGGCTCCCT GAGCTCAGGA AGATAGACCT CTCAGCAAAT
    GGCATCGGCC CAGCAGGCGG AGCGCGGTTG GCAGAATCTC TCACCCTTTG CAGGCACCTC
    GAGGAGCTGA TGCTTGGCTG CAATGCCCTG GGAGACTGCA CAGCCCTGGG TCTGGCCAAG
    GGGCTGCCCC AGCACCTGAG GGTCCTGCAC CTGCCATCCA GCCATCTGAG CCCAGAGGGG
    GTGCTGAGTC TGGGCCAGGC CCTGGATGGA TGCCCACACG TGGAAGAGAT CAGCTTGGCA
    GAGAACAGCC TGGCTGGAGG GGTCCCGCAT TTCCATCAGG GGTTCCCAGT GCTCCGGAAG
    CTTGACCTGG TGTCGTGTGA GATTGACAAC CAGACCGCCA AGCCCCTCGC CGCCAGCTTC
    GTGCTCTGTC TGGCCCTGGA AGAAATCACG CTGTCCTGGA ATCTGCTCGG GGATGAGGCA
    GCTGCTGAGC TGGCCCGGGT CCTGCCGCAG ATGGGCCGGC TGAAGAGAAT GGACCTGGAG
    AAAAATCAGA TCACAGCTTA TGGAGCCTGG CTCCTGGCTG AAGGGCTGGC ACAGGGATCT
    GGCATCCAAG TCATTCGCCT CTGGAATAAC CGTATTCCCC CGGACATGGC CCAGCATCTG
    CAGAACCAGG AGCCCAGGCT GGACTTTGCT TTCTTTGACA ACCAGCCACA GGTCATCCCT
    CGGGGTACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331288.1
    CDS552..5843
    Translation

    Target ORF information:

    RefSeq Version XM_015475802.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, CARD domain containing 5 (NLRC5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475802.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG AGCTGACGCA CTGTGCCCTG CAAGCCCAGC GCCTGGAGCT GCTCTGCAAA
    GCGCTGGGAG GAAGTTGCCG CCTCAGTCAC CTTGACTTAT CAGGCAATGC TCTGGGGGAT
    GAAGGTGTGG CCCTGCTGGC TCGGCTGCTC CCCGGACTGG GTTCTCTGCA GTCCTTGAAC
    CTCAGTGAGA ACCATTTGTC CCTGGAGGGT GTGTGCAGTT TGACCCAGTG CTTCTCTACT
    CTGCGGTGGC TTCTACATTT GGAATTCAGC TCTGCAAGCC AACACGTCAT CCTGAGAGGC
    GACAAAAGAG GCAGGGATCT GTTGGCTGCT GGATCTTTGC CAGAGTTCCG AGCTGGAGCC
    CGGTTGGGAT CTGGTCAGCG CCACGTCCCC AGGAGCTTCT GCCTCAAGGA ATGTCAGCTG
    GAGCCCCTGA GCCTCTCCTA CCTCTGCAAG TCTCTGGAGA AGTGCCCAGG GCCCCTGGAA
    GTCCAATTGT CCTGCGAGGT GCTGAGTGAC CAGAGCCTGG AGACCCTGCT GTACCACCTA
    CCGCAGCTCC CCCAGCTAAG CCTGCTGCAA CTGAGCCAGA TGCAACTGCC TCCAGAGAGC
    CCCCTCCTGC TAGCTGACCT CTTCAGCCTG TGCCCACAGG TTCAGAAGGT GGATCTCAGG
    TCCCTGCGTC ATTTGACCCT GCATCTCAGG TCTAGTGAGG AGCAGGAAGA CAGGTGCTGT
    TGGTTCACTG ACTGCGGCCT CCGCCAGGAG CACGTGGAGC CGCTGTGCGG GTTGCTGAGC
    AAGTGTGAAG ACCTCCACCA GCTGGACCTC TCAGCAAACC TGCTGGGTGA TGATGGGCTC
    AGGTGCCTCC TGGAATGTCT CCCCAAGGTG CCCATTTCCG GTTCGCTTGA TCTAAGCCAC
    AACAGTATGT CTCAGGAAAG CGTCTTGTGC CTGGTGGAGG CGCTCCCCTC CTGCCCACGT
    CTCCGGGAGG CCTCAGTGAA CCTGGGCTCC AAGCAGAGCT TCTGGATTCA CTTCTCCCGA
    CAGGAAGAGA CTGGGAAGAC ACTGAGGCTG AGCGAGTGCC ACTTCAAGCC AGAGCACATG
    CCCAGACTGG CCGCCAGCCT GAGCCAGGCC CTGCAGCTGA CGGAGCTCAC GCTGACCTGG
    TGTTTCCCGG GCCTGGAGCA GCTGACTGTC CTCCTGGGTC TGCTAACGTG GCCGACGGGG
    GTGCTCACTC TCAGGGTGGA AGAGCCATGG GTGGGCAGAG TCGGGGTGCT CACGCTGCTG
    GAAGTCTGTG CCCAGGCCTC AGGCAACATC ACTGGAATAA GCATCTCCGA GACCCAGAAA
    CAGCTCTGTA TCCAGCTGGA ATTTCCCCAT CAGGAGAACC CAGAAGCCGT GGCCCTCAGG
    CTGGCTCACT GTGACCTGGG AACCCAACAA AACCTCCTCG CCAGGAAGTT GATGGAGACA
    TGTGCCCGGC TGCGGCAGCT CAGTTTGTCC CAGGTGAACC TCTGCGATGC CAGCTCTCTG
    CTGCTGCAAA GCCTCCTGCT GTCCCTCTCT GAGCTGAAGA ACTTCAGGCT GACCTCCAGC
    TGTGTGAGCT CCAAGGGGCT AGCCCTCCTG ACATCTGGTC TGAGCCATTG TCACCTTCTG
    GAGGAGCTAG ACTTGTCTAA CAATCAACTT GGAGACGAGG GCACCAAGGT GTTGTTGGGG
    GCTCTTGAGG GCAAGTGCAG GCTGAAGAGA CTTGACCTCA GCCACCTGCC ACTGGGCGGC
    TCCACCCTGG CTGTGCTCAC TCAAGGACTA GGCCACATGA CACTCCTGCA GAGCCTCCGT
    CTGAGCAAGA GCAACATCGA TGACGTCGGC TGCTGCCACC TTTCGAAGGC TCTCAGAGCT
    GCCACCAGCT TGGAGGAGCT GAGCTTGAGC CACAACCAGA TCGGAAACAT CGGTGCCCAG
    GACTTAGCTG CTGTCCTGCC GGGGCTCCCT GAGCTCAGGA AGATAGACCT CTCAGCAAAT
    GGCATCGGCC CAGCAGGCGG AGCGCGGTTG GCAGAATCTC TCACCCTTTG CAGGCACCTC
    GAGGAGCTGA TGCTTGGCTG CAATGCCCTG GGAGACTGCA CAGCCCTGGG TCTGGCCAAG
    GGGCTGCCCC AGCACCTGAG GGTCCTGCAC CTGCCATCCA GCCATCTGAG CCCAGAGGGG
    GTGCTGAGTC TGGGCCAGGC CCTGGATGGA TGCCCACACG TGGAAGAGAT CAGCTTGGCA
    GAGAACAGCC TGGCTGGAGG GGTCCCGCAT TTCCATCAGG GGTTCCCAGT GCTCCGGAAG
    CTTGACCTGG TGTCGTGTGA GATTGACAAC CAGACCGCCA AGCCCCTCGC CGCCAGCTTC
    GTGCTCTGTC TGGCCCTGGA AGAAATCACG CTGTCCTGGA ATCTGCTCGG GGATGAGGCA
    GCTGCTGAGC TGGCCCGGGT CCTGCCGCAG ATGGGCCGGC TGAAGAGAAT GGACCTGGAG
    AAAAATCAGA TCACAGCTTA TGGAGCCTGG CTCCTGGCTG AAGGGCTGGC ACAGGGATCT
    GGCATCCAAG TCATTCGCCT CTGGAATAAC CGTATTCCCC CGGACATGGC CCAGCATCTG
    CAGAACCAGG AGCCCAGGCT GGACTTTGCT TTCTTTGACA ACCAGCCACA GGTCATCCCT
    CGGGGTACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09294
    Clone ID Related Accession (Same CDS sequence) XM_005218805.3 , XM_005195224.3 , XM_005218805.4 , XM_024978951.1 , XM_024978950.1
    Accession Version XM_005218805.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein NLRC5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218805.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG ACCTCTCAGG GAACCAGCTC AAAGATAAAG GCTGTCAACT GATGGCAGAG
    GCTGCTCCCC AACTGCACAT CACGAAGAAG CTGGACCTCA GCGACAATGA GCTTTCTGTG
    GCCGGGGTGC TGGGAGTACT GAGTGTGGTG AACACGTACC AAACCCTGAC AGAGCTACAT
    ATCAGCCTGC TGCACAAAAC TGTGGTCTTC ACGTTTGCCC CAGAGCTGGA GGAGCAGGAG
    GGGATCCAGA AGAGGGCTAC GTTTCCTGAC AGCCTTATGT TCCAGATGTC CTCTGAGCCA
    TCTCTGCGCT CCCCGAGGAT CAGGCTGACG CACTGTGCCC TGCAAGCCCA GCGCCTGGAG
    CTGCTCTGCA AAGCGCTGGG AGGAAGTTGC CGCCTCAGTC ACCTTGACTT ATCAGGCAAT
    GCTCTGGGGG ATGAAGGTGT GGCCCTGCTG GCTCGGCTGC TCCCCGGACT GGGTTCTCTG
    CAGTCCTTGA ACCTCAGTGA GAACCATTTG TCCCTGGAGG GTGTGTGCAG TTTGACCCAG
    TGCTTCTCTA CTCTGCGGTG GCTTCTACAT TTGGAATTCA GCTCTGCAAG CCAACACGTC
    ATCCTGAGAG GCGACAAAAG AGGCAGGGAT CTGTTGGCTG CTGGATCTTT GCCAGAGTTC
    CGAGCTGGAG CCCGGTTGGG ATCTGGTCAG CGCCACGTCC CCAGGAGCTT CTGCCTCAAG
    GAATGTCAGC TGGAGCCCCT GAGCCTCTCC TACCTCTGCA AGTCTCTGGA GAAGTGCCCA
    GGGCCCCTGG AAGTCCAATT GTCCTGCGAG GTGCTGAGTG ACCAGAGCCT GGAGACCCTG
    CTGTACCACC TACCGCAGCT CCCCCAGCTA AGCCTGCTGC AACTGAGCCA GATGCAACTG
    CCTCCAGAGA GCCCCCTCCT GCTAGCTGAC CTCTTCAGCC TGTGCCCACA GGTTCAGAAG
    GTGGATCTCA GGTCCCTGCG TCATTTGACC CTGCATCTCA GGTCTAGTGA GGAGCAGGAA
    GACAGGTGCT GTTGGTTCAC TGACTGCGGC CTCCGCCAGG AGCACGTGGA GCCGCTGTGC
    GGGTTGCTGA GCAAGTGTGA AGACCTCCAC CAGCTGGACC TCTCAGCAAA CCTGCTGGGT
    GATGATGGGC TCAGGTGCCT CCTGGAATGT CTCCCCAAGG TGCCCATTTC CGGTTCGCTT
    GATCTAAGCC ACAACAGTAT GTCTCAGGAA AGCGTCTTGT GCCTGGTGGA GGCGCTCCCC
    TCCTGCCCAC GTCTCCGGGA GGCCTCAGTG AACCTGGGCT CCAAGCAGAG CTTCTGGATT
    CACTTCTCCC GACAGGAAGA GACTGGGAAG ACACTGAGGC TGAGCGAGTG CCACTTCAAG
    CCAGAGCACA TGCCCAGACT GGCCGCCAGC CTGAGCCAGG CCCTGCAGCT GACGGAGCTC
    ACGCTGACCT GGTGTTTCCC GGGCCTGGAG CAGCTGACTG TCCTCCTGGG TCTGCTAACG
    TGGCCGACGG GGGTGCTCAC TCTCAGGGTG GAAGAGCCAT GGGTGGGCAG AGTCGGGGTG
    CTCACGCTGC TGGAAGTCTG TGCCCAGGCC TCAGGCAACA TCACTGGAAT AAGCATCTCC
    GAGACCCAGA AACAGCTCTG TATCCAGCTG GAATTTCCCC ATCAGGAGAA CCCAGAAGCC
    GTGGCCCTCA GGCTGGCTCA CTGTGACCTG GGAACCCAAC AAAACCTCCT CGCCAGGAAG
    TTGATGGAGA CATGTGCCCG GCTGCGGCAG CTCAGTTTGT CCCAGGTGAA CCTCTGCGAT
    GCCAGCTCTC TGCTGCTGCA AAGCCTCCTG CTGTCCCTCT CTGAGCTGAA GAACTTCAGG
    CTGACCTCCA GCTGTGTGAG CTCCAAGGGG CTAGCCCTCC TGACATCTGG TCTGAGCCAT
    TGTCACCTTC TGGAGGAGCT AGACTTGTCT AACAATCAAC TTGGAGACGA GGGCACCAAG
    GTGTTGTTGG GGGCTCTTGA GGGCAAGTGC AGGCTGAAGA GACTTGACCT CAGCCACCTG
    CCACTGGGCG GCTCCACCCT GGCTGTGCTC ACTCAAGGAC TAGGCCACAT GACACTCCTG
    CAGAGCCTCC GTCTGAGCAA GAGCAACATC GATGACGTCG GCTGCTGCCA CCTTTCGAAG
    GCTCTCAGAG CTGCCACCAG CTTGGAGGAG CTGAGCTTGA GCCACAACCA GATCGGAAAC
    ATCGGTGCCC AGGACTTAGC TGCTGTCCTG CCGGGGCTCC CTGAGCTCAG GAAGATAGAC
    CTCTCAGCAA ATGGCATCGG CCCAGCAGGC GGAGCGCGGT TGGCAGAATC TCTCACCCTT
    TGCAGGCACC TCGAGGAGCT GATGCTTGGC TGCAATGCCC TGGGAGACTG CACAGCCCTG
    GGTCTGGCCA AGGGGCTGCC CCAGCACCTG AGGGTCCTGC ACCTGCCATC CAGCCATCTG
    AGCCCAGAGG GGGTGCTGAG TCTGGGCCAG GCCCTGGATG GATGCCCACA CGTGGAAGAG
    ATCAGCTTGG CAGAGAACAG CCTGGCTGGA GGGGTCCCGC ATTTCCATCA GGGGTTCCCA
    GTGCTCCGGA AGCTTGACCT GGTGTCGTGT GAGATTGACA ACCAGACCGC CAAGCCCCTC
    GCCGCCAGCT TCGTGCTCTG TCTGGCCCTG GAAGAAATCA CGCTGTCCTG GAATCTGCTC
    GGGGATGAGG CAGCTGCTGA GCTGGCCCGG GTCCTGCCGC AGATGGGCCG GCTGAAGAGA
    ATGGACCTGG AGAAAAATCA GATCACAGCT TATGGAGCCT GGCTCCTGGC TGAAGGGCTG
    GCACAGGGAT CTGGCATCCA AGTCATTCGC CTCTGGAATA ACCGTATTCC CCCGGACATG
    GCCCAGCATC TGCAGAACCA GGAGCCCAGG CTGGACTTTG CTTTCTTTGA CAACCAGCCA
    CAGGTCATCC CTCGGGGTAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218862.1
    CDS552..6155
    Translation

    Target ORF information:

    RefSeq Version XM_005218805.3
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, CARD domain containing 5 (NLRC5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218805.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG ACCTCTCAGG GAACCAGCTC AAAGATAAAG GCTGTCAACT GATGGCAGAG
    GCTGCTCCCC AACTGCACAT CACGAAGAAG CTGGACCTCA GCGACAATGA GCTTTCTGTG
    GCCGGGGTGC TGGGAGTACT GAGTGTGGTG AACACGTACC AAACCCTGAC AGAGCTACAT
    ATCAGCCTGC TGCACAAAAC TGTGGTCTTC ACGTTTGCCC CAGAGCTGGA GGAGCAGGAG
    GGGATCCAGA AGAGGGCTAC GTTTCCTGAC AGCCTTATGT TCCAGATGTC CTCTGAGCCA
    TCTCTGCGCT CCCCGAGGAT CAGGCTGACG CACTGTGCCC TGCAAGCCCA GCGCCTGGAG
    CTGCTCTGCA AAGCGCTGGG AGGAAGTTGC CGCCTCAGTC ACCTTGACTT ATCAGGCAAT
    GCTCTGGGGG ATGAAGGTGT GGCCCTGCTG GCTCGGCTGC TCCCCGGACT GGGTTCTCTG
    CAGTCCTTGA ACCTCAGTGA GAACCATTTG TCCCTGGAGG GTGTGTGCAG TTTGACCCAG
    TGCTTCTCTA CTCTGCGGTG GCTTCTACAT TTGGAATTCA GCTCTGCAAG CCAACACGTC
    ATCCTGAGAG GCGACAAAAG AGGCAGGGAT CTGTTGGCTG CTGGATCTTT GCCAGAGTTC
    CGAGCTGGAG CCCGGTTGGG ATCTGGTCAG CGCCACGTCC CCAGGAGCTT CTGCCTCAAG
    GAATGTCAGC TGGAGCCCCT GAGCCTCTCC TACCTCTGCA AGTCTCTGGA GAAGTGCCCA
    GGGCCCCTGG AAGTCCAATT GTCCTGCGAG GTGCTGAGTG ACCAGAGCCT GGAGACCCTG
    CTGTACCACC TACCGCAGCT CCCCCAGCTA AGCCTGCTGC AACTGAGCCA GATGCAACTG
    CCTCCAGAGA GCCCCCTCCT GCTAGCTGAC CTCTTCAGCC TGTGCCCACA GGTTCAGAAG
    GTGGATCTCA GGTCCCTGCG TCATTTGACC CTGCATCTCA GGTCTAGTGA GGAGCAGGAA
    GACAGGTGCT GTTGGTTCAC TGACTGCGGC CTCCGCCAGG AGCACGTGGA GCCGCTGTGC
    GGGTTGCTGA GCAAGTGTGA AGACCTCCAC CAGCTGGACC TCTCAGCAAA CCTGCTGGGT
    GATGATGGGC TCAGGTGCCT CCTGGAATGT CTCCCCAAGG TGCCCATTTC CGGTTCGCTT
    GATCTAAGCC ACAACAGTAT GTCTCAGGAA AGCGTCTTGT GCCTGGTGGA GGCGCTCCCC
    TCCTGCCCAC GTCTCCGGGA GGCCTCAGTG AACCTGGGCT CCAAGCAGAG CTTCTGGATT
    CACTTCTCCC GACAGGAAGA GACTGGGAAG ACACTGAGGC TGAGCGAGTG CCACTTCAAG
    CCAGAGCACA TGCCCAGACT GGCCGCCAGC CTGAGCCAGG CCCTGCAGCT GACGGAGCTC
    ACGCTGACCT GGTGTTTCCC GGGCCTGGAG CAGCTGACTG TCCTCCTGGG TCTGCTAACG
    TGGCCGACGG GGGTGCTCAC TCTCAGGGTG GAAGAGCCAT GGGTGGGCAG AGTCGGGGTG
    CTCACGCTGC TGGAAGTCTG TGCCCAGGCC TCAGGCAACA TCACTGGAAT AAGCATCTCC
    GAGACCCAGA AACAGCTCTG TATCCAGCTG GAATTTCCCC ATCAGGAGAA CCCAGAAGCC
    GTGGCCCTCA GGCTGGCTCA CTGTGACCTG GGAACCCAAC AAAACCTCCT CGCCAGGAAG
    TTGATGGAGA CATGTGCCCG GCTGCGGCAG CTCAGTTTGT CCCAGGTGAA CCTCTGCGAT
    GCCAGCTCTC TGCTGCTGCA AAGCCTCCTG CTGTCCCTCT CTGAGCTGAA GAACTTCAGG
    CTGACCTCCA GCTGTGTGAG CTCCAAGGGG CTAGCCCTCC TGACATCTGG TCTGAGCCAT
    TGTCACCTTC TGGAGGAGCT AGACTTGTCT AACAATCAAC TTGGAGACGA GGGCACCAAG
    GTGTTGTTGG GGGCTCTTGA GGGCAAGTGC AGGCTGAAGA GACTTGACCT CAGCCACCTG
    CCACTGGGCG GCTCCACCCT GGCTGTGCTC ACTCAAGGAC TAGGCCACAT GACACTCCTG
    CAGAGCCTCC GTCTGAGCAA GAGCAACATC GATGACGTCG GCTGCTGCCA CCTTTCGAAG
    GCTCTCAGAG CTGCCACCAG CTTGGAGGAG CTGAGCTTGA GCCACAACCA GATCGGAAAC
    ATCGGTGCCC AGGACTTAGC TGCTGTCCTG CCGGGGCTCC CTGAGCTCAG GAAGATAGAC
    CTCTCAGCAA ATGGCATCGG CCCAGCAGGC GGAGCGCGGT TGGCAGAATC TCTCACCCTT
    TGCAGGCACC TCGAGGAGCT GATGCTTGGC TGCAATGCCC TGGGAGACTG CACAGCCCTG
    GGTCTGGCCA AGGGGCTGCC CCAGCACCTG AGGGTCCTGC ACCTGCCATC CAGCCATCTG
    AGCCCAGAGG GGGTGCTGAG TCTGGGCCAG GCCCTGGATG GATGCCCACA CGTGGAAGAG
    ATCAGCTTGG CAGAGAACAG CCTGGCTGGA GGGGTCCCGC ATTTCCATCA GGGGTTCCCA
    GTGCTCCGGA AGCTTGACCT GGTGTCGTGT GAGATTGACA ACCAGACCGC CAAGCCCCTC
    GCCGCCAGCT TCGTGCTCTG TCTGGCCCTG GAAGAAATCA CGCTGTCCTG GAATCTGCTC
    GGGGATGAGG CAGCTGCTGA GCTGGCCCGG GTCCTGCCGC AGATGGGCCG GCTGAAGAGA
    ATGGACCTGG AGAAAAATCA GATCACAGCT TATGGAGCCT GGCTCCTGGC TGAAGGGCTG
    GCACAGGGAT CTGGCATCCA AGTCATTCGC CTCTGGAATA ACCGTATTCC CCCGGACATG
    GCCCAGCATC TGCAGAACCA GGAGCCCAGG CTGGACTTTG CTTTCTTTGA CAACCAGCCA
    CAGGTCATCC CTCGGGGTAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09294
    Clone ID Related Accession (Same CDS sequence) XM_005218805.3 , XM_005195224.3 , XM_005218805.4 , XM_024978951.1 , XM_024978950.1
    Accession Version XM_005195224.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein NLRC5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195224.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG ACCTCTCAGG GAACCAGCTC AAAGATAAAG GCTGTCAACT GATGGCAGAG
    GCTGCTCCCC AACTGCACAT CACGAAGAAG CTGGACCTCA GCGACAATGA GCTTTCTGTG
    GCCGGGGTGC TGGGAGTACT GAGTGTGGTG AACACGTACC AAACCCTGAC AGAGCTACAT
    ATCAGCCTGC TGCACAAAAC TGTGGTCTTC ACGTTTGCCC CAGAGCTGGA GGAGCAGGAG
    GGGATCCAGA AGAGGGCTAC GTTTCCTGAC AGCCTTATGT TCCAGATGTC CTCTGAGCCA
    TCTCTGCGCT CCCCGAGGAT CAGGCTGACG CACTGTGCCC TGCAAGCCCA GCGCCTGGAG
    CTGCTCTGCA AAGCGCTGGG AGGAAGTTGC CGCCTCAGTC ACCTTGACTT ATCAGGCAAT
    GCTCTGGGGG ATGAAGGTGT GGCCCTGCTG GCTCGGCTGC TCCCCGGACT GGGTTCTCTG
    CAGTCCTTGA ACCTCAGTGA GAACCATTTG TCCCTGGAGG GTGTGTGCAG TTTGACCCAG
    TGCTTCTCTA CTCTGCGGTG GCTTCTACAT TTGGAATTCA GCTCTGCAAG CCAACACGTC
    ATCCTGAGAG GCGACAAAAG AGGCAGGGAT CTGTTGGCTG CTGGATCTTT GCCAGAGTTC
    CGAGCTGGAG CCCGGTTGGG ATCTGGTCAG CGCCACGTCC CCAGGAGCTT CTGCCTCAAG
    GAATGTCAGC TGGAGCCCCT GAGCCTCTCC TACCTCTGCA AGTCTCTGGA GAAGTGCCCA
    GGGCCCCTGG AAGTCCAATT GTCCTGCGAG GTGCTGAGTG ACCAGAGCCT GGAGACCCTG
    CTGTACCACC TACCGCAGCT CCCCCAGCTA AGCCTGCTGC AACTGAGCCA GATGCAACTG
    CCTCCAGAGA GCCCCCTCCT GCTAGCTGAC CTCTTCAGCC TGTGCCCACA GGTTCAGAAG
    GTGGATCTCA GGTCCCTGCG TCATTTGACC CTGCATCTCA GGTCTAGTGA GGAGCAGGAA
    GACAGGTGCT GTTGGTTCAC TGACTGCGGC CTCCGCCAGG AGCACGTGGA GCCGCTGTGC
    GGGTTGCTGA GCAAGTGTGA AGACCTCCAC CAGCTGGACC TCTCAGCAAA CCTGCTGGGT
    GATGATGGGC TCAGGTGCCT CCTGGAATGT CTCCCCAAGG TGCCCATTTC CGGTTCGCTT
    GATCTAAGCC ACAACAGTAT GTCTCAGGAA AGCGTCTTGT GCCTGGTGGA GGCGCTCCCC
    TCCTGCCCAC GTCTCCGGGA GGCCTCAGTG AACCTGGGCT CCAAGCAGAG CTTCTGGATT
    CACTTCTCCC GACAGGAAGA GACTGGGAAG ACACTGAGGC TGAGCGAGTG CCACTTCAAG
    CCAGAGCACA TGCCCAGACT GGCCGCCAGC CTGAGCCAGG CCCTGCAGCT GACGGAGCTC
    ACGCTGACCT GGTGTTTCCC GGGCCTGGAG CAGCTGACTG TCCTCCTGGG TCTGCTAACG
    TGGCCGACGG GGGTGCTCAC TCTCAGGGTG GAAGAGCCAT GGGTGGGCAG AGTCGGGGTG
    CTCACGCTGC TGGAAGTCTG TGCCCAGGCC TCAGGCAACA TCACTGGAAT AAGCATCTCC
    GAGACCCAGA AACAGCTCTG TATCCAGCTG GAATTTCCCC ATCAGGAGAA CCCAGAAGCC
    GTGGCCCTCA GGCTGGCTCA CTGTGACCTG GGAACCCAAC AAAACCTCCT CGCCAGGAAG
    TTGATGGAGA CATGTGCCCG GCTGCGGCAG CTCAGTTTGT CCCAGGTGAA CCTCTGCGAT
    GCCAGCTCTC TGCTGCTGCA AAGCCTCCTG CTGTCCCTCT CTGAGCTGAA GAACTTCAGG
    CTGACCTCCA GCTGTGTGAG CTCCAAGGGG CTAGCCCTCC TGACATCTGG TCTGAGCCAT
    TGTCACCTTC TGGAGGAGCT AGACTTGTCT AACAATCAAC TTGGAGACGA GGGCACCAAG
    GTGTTGTTGG GGGCTCTTGA GGGCAAGTGC AGGCTGAAGA GACTTGACCT CAGCCACCTG
    CCACTGGGCG GCTCCACCCT GGCTGTGCTC ACTCAAGGAC TAGGCCACAT GACACTCCTG
    CAGAGCCTCC GTCTGAGCAA GAGCAACATC GATGACGTCG GCTGCTGCCA CCTTTCGAAG
    GCTCTCAGAG CTGCCACCAG CTTGGAGGAG CTGAGCTTGA GCCACAACCA GATCGGAAAC
    ATCGGTGCCC AGGACTTAGC TGCTGTCCTG CCGGGGCTCC CTGAGCTCAG GAAGATAGAC
    CTCTCAGCAA ATGGCATCGG CCCAGCAGGC GGAGCGCGGT TGGCAGAATC TCTCACCCTT
    TGCAGGCACC TCGAGGAGCT GATGCTTGGC TGCAATGCCC TGGGAGACTG CACAGCCCTG
    GGTCTGGCCA AGGGGCTGCC CCAGCACCTG AGGGTCCTGC ACCTGCCATC CAGCCATCTG
    AGCCCAGAGG GGGTGCTGAG TCTGGGCCAG GCCCTGGATG GATGCCCACA CGTGGAAGAG
    ATCAGCTTGG CAGAGAACAG CCTGGCTGGA GGGGTCCCGC ATTTCCATCA GGGGTTCCCA
    GTGCTCCGGA AGCTTGACCT GGTGTCGTGT GAGATTGACA ACCAGACCGC CAAGCCCCTC
    GCCGCCAGCT TCGTGCTCTG TCTGGCCCTG GAAGAAATCA CGCTGTCCTG GAATCTGCTC
    GGGGATGAGG CAGCTGCTGA GCTGGCCCGG GTCCTGCCGC AGATGGGCCG GCTGAAGAGA
    ATGGACCTGG AGAAAAATCA GATCACAGCT TATGGAGCCT GGCTCCTGGC TGAAGGGCTG
    GCACAGGGAT CTGGCATCCA AGTCATTCGC CTCTGGAATA ACCGTATTCC CCCGGACATG
    GCCCAGCATC TGCAGAACCA GGAGCCCAGG CTGGACTTTG CTTTCTTTGA CAACCAGCCA
    CAGGTCATCC CTCGGGGTAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005195281.1
    CDS552..6155
    Translation

    Target ORF information:

    RefSeq Version XM_005195224.3
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, CARD domain containing 5 (NLRC5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005195224.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG ACCTCTCAGG GAACCAGCTC AAAGATAAAG GCTGTCAACT GATGGCAGAG
    GCTGCTCCCC AACTGCACAT CACGAAGAAG CTGGACCTCA GCGACAATGA GCTTTCTGTG
    GCCGGGGTGC TGGGAGTACT GAGTGTGGTG AACACGTACC AAACCCTGAC AGAGCTACAT
    ATCAGCCTGC TGCACAAAAC TGTGGTCTTC ACGTTTGCCC CAGAGCTGGA GGAGCAGGAG
    GGGATCCAGA AGAGGGCTAC GTTTCCTGAC AGCCTTATGT TCCAGATGTC CTCTGAGCCA
    TCTCTGCGCT CCCCGAGGAT CAGGCTGACG CACTGTGCCC TGCAAGCCCA GCGCCTGGAG
    CTGCTCTGCA AAGCGCTGGG AGGAAGTTGC CGCCTCAGTC ACCTTGACTT ATCAGGCAAT
    GCTCTGGGGG ATGAAGGTGT GGCCCTGCTG GCTCGGCTGC TCCCCGGACT GGGTTCTCTG
    CAGTCCTTGA ACCTCAGTGA GAACCATTTG TCCCTGGAGG GTGTGTGCAG TTTGACCCAG
    TGCTTCTCTA CTCTGCGGTG GCTTCTACAT TTGGAATTCA GCTCTGCAAG CCAACACGTC
    ATCCTGAGAG GCGACAAAAG AGGCAGGGAT CTGTTGGCTG CTGGATCTTT GCCAGAGTTC
    CGAGCTGGAG CCCGGTTGGG ATCTGGTCAG CGCCACGTCC CCAGGAGCTT CTGCCTCAAG
    GAATGTCAGC TGGAGCCCCT GAGCCTCTCC TACCTCTGCA AGTCTCTGGA GAAGTGCCCA
    GGGCCCCTGG AAGTCCAATT GTCCTGCGAG GTGCTGAGTG ACCAGAGCCT GGAGACCCTG
    CTGTACCACC TACCGCAGCT CCCCCAGCTA AGCCTGCTGC AACTGAGCCA GATGCAACTG
    CCTCCAGAGA GCCCCCTCCT GCTAGCTGAC CTCTTCAGCC TGTGCCCACA GGTTCAGAAG
    GTGGATCTCA GGTCCCTGCG TCATTTGACC CTGCATCTCA GGTCTAGTGA GGAGCAGGAA
    GACAGGTGCT GTTGGTTCAC TGACTGCGGC CTCCGCCAGG AGCACGTGGA GCCGCTGTGC
    GGGTTGCTGA GCAAGTGTGA AGACCTCCAC CAGCTGGACC TCTCAGCAAA CCTGCTGGGT
    GATGATGGGC TCAGGTGCCT CCTGGAATGT CTCCCCAAGG TGCCCATTTC CGGTTCGCTT
    GATCTAAGCC ACAACAGTAT GTCTCAGGAA AGCGTCTTGT GCCTGGTGGA GGCGCTCCCC
    TCCTGCCCAC GTCTCCGGGA GGCCTCAGTG AACCTGGGCT CCAAGCAGAG CTTCTGGATT
    CACTTCTCCC GACAGGAAGA GACTGGGAAG ACACTGAGGC TGAGCGAGTG CCACTTCAAG
    CCAGAGCACA TGCCCAGACT GGCCGCCAGC CTGAGCCAGG CCCTGCAGCT GACGGAGCTC
    ACGCTGACCT GGTGTTTCCC GGGCCTGGAG CAGCTGACTG TCCTCCTGGG TCTGCTAACG
    TGGCCGACGG GGGTGCTCAC TCTCAGGGTG GAAGAGCCAT GGGTGGGCAG AGTCGGGGTG
    CTCACGCTGC TGGAAGTCTG TGCCCAGGCC TCAGGCAACA TCACTGGAAT AAGCATCTCC
    GAGACCCAGA AACAGCTCTG TATCCAGCTG GAATTTCCCC ATCAGGAGAA CCCAGAAGCC
    GTGGCCCTCA GGCTGGCTCA CTGTGACCTG GGAACCCAAC AAAACCTCCT CGCCAGGAAG
    TTGATGGAGA CATGTGCCCG GCTGCGGCAG CTCAGTTTGT CCCAGGTGAA CCTCTGCGAT
    GCCAGCTCTC TGCTGCTGCA AAGCCTCCTG CTGTCCCTCT CTGAGCTGAA GAACTTCAGG
    CTGACCTCCA GCTGTGTGAG CTCCAAGGGG CTAGCCCTCC TGACATCTGG TCTGAGCCAT
    TGTCACCTTC TGGAGGAGCT AGACTTGTCT AACAATCAAC TTGGAGACGA GGGCACCAAG
    GTGTTGTTGG GGGCTCTTGA GGGCAAGTGC AGGCTGAAGA GACTTGACCT CAGCCACCTG
    CCACTGGGCG GCTCCACCCT GGCTGTGCTC ACTCAAGGAC TAGGCCACAT GACACTCCTG
    CAGAGCCTCC GTCTGAGCAA GAGCAACATC GATGACGTCG GCTGCTGCCA CCTTTCGAAG
    GCTCTCAGAG CTGCCACCAG CTTGGAGGAG CTGAGCTTGA GCCACAACCA GATCGGAAAC
    ATCGGTGCCC AGGACTTAGC TGCTGTCCTG CCGGGGCTCC CTGAGCTCAG GAAGATAGAC
    CTCTCAGCAA ATGGCATCGG CCCAGCAGGC GGAGCGCGGT TGGCAGAATC TCTCACCCTT
    TGCAGGCACC TCGAGGAGCT GATGCTTGGC TGCAATGCCC TGGGAGACTG CACAGCCCTG
    GGTCTGGCCA AGGGGCTGCC CCAGCACCTG AGGGTCCTGC ACCTGCCATC CAGCCATCTG
    AGCCCAGAGG GGGTGCTGAG TCTGGGCCAG GCCCTGGATG GATGCCCACA CGTGGAAGAG
    ATCAGCTTGG CAGAGAACAG CCTGGCTGGA GGGGTCCCGC ATTTCCATCA GGGGTTCCCA
    GTGCTCCGGA AGCTTGACCT GGTGTCGTGT GAGATTGACA ACCAGACCGC CAAGCCCCTC
    GCCGCCAGCT TCGTGCTCTG TCTGGCCCTG GAAGAAATCA CGCTGTCCTG GAATCTGCTC
    GGGGATGAGG CAGCTGCTGA GCTGGCCCGG GTCCTGCCGC AGATGGGCCG GCTGAAGAGA
    ATGGACCTGG AGAAAAATCA GATCACAGCT TATGGAGCCT GGCTCCTGGC TGAAGGGCTG
    GCACAGGGAT CTGGCATCCA AGTCATTCGC CTCTGGAATA ACCGTATTCC CCCGGACATG
    GCCCAGCATC TGCAGAACCA GGAGCCCAGG CTGGACTTTG CTTTCTTTGA CAACCAGCCA
    CAGGTCATCC CTCGGGGTAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09294
    Clone ID Related Accession (Same CDS sequence) XM_005218805.3 , XM_005195224.3 , XM_005218805.4 , XM_024978951.1 , XM_024978950.1
    Accession Version XM_005218805.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein NLRC5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218805.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG ACCTCTCAGG GAACCAGCTC AAAGATAAAG GCTGTCAACT GATGGCAGAG
    GCTGCTCCCC AACTGCACAT CACGAAGAAG CTGGACCTCA GCGACAATGA GCTTTCTGTG
    GCCGGGGTGC TGGGAGTACT GAGTGTGGTG AACACGTACC AAACCCTGAC AGAGCTACAT
    ATCAGCCTGC TGCACAAAAC TGTGGTCTTC ACGTTTGCCC CAGAGCTGGA GGAGCAGGAG
    GGGATCCAGA AGAGGGCTAC GTTTCCTGAC AGCCTTATGT TCCAGATGTC CTCTGAGCCA
    TCTCTGCGCT CCCCGAGGAT CAGGCTGACG CACTGTGCCC TGCAAGCCCA GCGCCTGGAG
    CTGCTCTGCA AAGCGCTGGG AGGAAGTTGC CGCCTCAGTC ACCTTGACTT ATCAGGCAAT
    GCTCTGGGGG ATGAAGGTGT GGCCCTGCTG GCTCGGCTGC TCCCCGGACT GGGTTCTCTG
    CAGTCCTTGA ACCTCAGTGA GAACCATTTG TCCCTGGAGG GTGTGTGCAG TTTGACCCAG
    TGCTTCTCTA CTCTGCGGTG GCTTCTACAT TTGGAATTCA GCTCTGCAAG CCAACACGTC
    ATCCTGAGAG GCGACAAAAG AGGCAGGGAT CTGTTGGCTG CTGGATCTTT GCCAGAGTTC
    CGAGCTGGAG CCCGGTTGGG ATCTGGTCAG CGCCACGTCC CCAGGAGCTT CTGCCTCAAG
    GAATGTCAGC TGGAGCCCCT GAGCCTCTCC TACCTCTGCA AGTCTCTGGA GAAGTGCCCA
    GGGCCCCTGG AAGTCCAATT GTCCTGCGAG GTGCTGAGTG ACCAGAGCCT GGAGACCCTG
    CTGTACCACC TACCGCAGCT CCCCCAGCTA AGCCTGCTGC AACTGAGCCA GATGCAACTG
    CCTCCAGAGA GCCCCCTCCT GCTAGCTGAC CTCTTCAGCC TGTGCCCACA GGTTCAGAAG
    GTGGATCTCA GGTCCCTGCG TCATTTGACC CTGCATCTCA GGTCTAGTGA GGAGCAGGAA
    GACAGGTGCT GTTGGTTCAC TGACTGCGGC CTCCGCCAGG AGCACGTGGA GCCGCTGTGC
    GGGTTGCTGA GCAAGTGTGA AGACCTCCAC CAGCTGGACC TCTCAGCAAA CCTGCTGGGT
    GATGATGGGC TCAGGTGCCT CCTGGAATGT CTCCCCAAGG TGCCCATTTC CGGTTCGCTT
    GATCTAAGCC ACAACAGTAT GTCTCAGGAA AGCGTCTTGT GCCTGGTGGA GGCGCTCCCC
    TCCTGCCCAC GTCTCCGGGA GGCCTCAGTG AACCTGGGCT CCAAGCAGAG CTTCTGGATT
    CACTTCTCCC GACAGGAAGA GACTGGGAAG ACACTGAGGC TGAGCGAGTG CCACTTCAAG
    CCAGAGCACA TGCCCAGACT GGCCGCCAGC CTGAGCCAGG CCCTGCAGCT GACGGAGCTC
    ACGCTGACCT GGTGTTTCCC GGGCCTGGAG CAGCTGACTG TCCTCCTGGG TCTGCTAACG
    TGGCCGACGG GGGTGCTCAC TCTCAGGGTG GAAGAGCCAT GGGTGGGCAG AGTCGGGGTG
    CTCACGCTGC TGGAAGTCTG TGCCCAGGCC TCAGGCAACA TCACTGGAAT AAGCATCTCC
    GAGACCCAGA AACAGCTCTG TATCCAGCTG GAATTTCCCC ATCAGGAGAA CCCAGAAGCC
    GTGGCCCTCA GGCTGGCTCA CTGTGACCTG GGAACCCAAC AAAACCTCCT CGCCAGGAAG
    TTGATGGAGA CATGTGCCCG GCTGCGGCAG CTCAGTTTGT CCCAGGTGAA CCTCTGCGAT
    GCCAGCTCTC TGCTGCTGCA AAGCCTCCTG CTGTCCCTCT CTGAGCTGAA GAACTTCAGG
    CTGACCTCCA GCTGTGTGAG CTCCAAGGGG CTAGCCCTCC TGACATCTGG TCTGAGCCAT
    TGTCACCTTC TGGAGGAGCT AGACTTGTCT AACAATCAAC TTGGAGACGA GGGCACCAAG
    GTGTTGTTGG GGGCTCTTGA GGGCAAGTGC AGGCTGAAGA GACTTGACCT CAGCCACCTG
    CCACTGGGCG GCTCCACCCT GGCTGTGCTC ACTCAAGGAC TAGGCCACAT GACACTCCTG
    CAGAGCCTCC GTCTGAGCAA GAGCAACATC GATGACGTCG GCTGCTGCCA CCTTTCGAAG
    GCTCTCAGAG CTGCCACCAG CTTGGAGGAG CTGAGCTTGA GCCACAACCA GATCGGAAAC
    ATCGGTGCCC AGGACTTAGC TGCTGTCCTG CCGGGGCTCC CTGAGCTCAG GAAGATAGAC
    CTCTCAGCAA ATGGCATCGG CCCAGCAGGC GGAGCGCGGT TGGCAGAATC TCTCACCCTT
    TGCAGGCACC TCGAGGAGCT GATGCTTGGC TGCAATGCCC TGGGAGACTG CACAGCCCTG
    GGTCTGGCCA AGGGGCTGCC CCAGCACCTG AGGGTCCTGC ACCTGCCATC CAGCCATCTG
    AGCCCAGAGG GGGTGCTGAG TCTGGGCCAG GCCCTGGATG GATGCCCACA CGTGGAAGAG
    ATCAGCTTGG CAGAGAACAG CCTGGCTGGA GGGGTCCCGC ATTTCCATCA GGGGTTCCCA
    GTGCTCCGGA AGCTTGACCT GGTGTCGTGT GAGATTGACA ACCAGACCGC CAAGCCCCTC
    GCCGCCAGCT TCGTGCTCTG TCTGGCCCTG GAAGAAATCA CGCTGTCCTG GAATCTGCTC
    GGGGATGAGG CAGCTGCTGA GCTGGCCCGG GTCCTGCCGC AGATGGGCCG GCTGAAGAGA
    ATGGACCTGG AGAAAAATCA GATCACAGCT TATGGAGCCT GGCTCCTGGC TGAAGGGCTG
    GCACAGGGAT CTGGCATCCA AGTCATTCGC CTCTGGAATA ACCGTATTCC CCCGGACATG
    GCCCAGCATC TGCAGAACCA GGAGCCCAGG CTGGACTTTG CTTTCTTTGA CAACCAGCCA
    CAGGTCATCC CTCGGGGTAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218862.1
    CDS582..6185
    Translation

    Target ORF information:

    RefSeq Version XM_005218805.4
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family CARD domain containing 5 (NLRC5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218805.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG ACCTCTCAGG GAACCAGCTC AAAGATAAAG GCTGTCAACT GATGGCAGAG
    GCTGCTCCCC AACTGCACAT CACGAAGAAG CTGGACCTCA GCGACAATGA GCTTTCTGTG
    GCCGGGGTGC TGGGAGTACT GAGTGTGGTG AACACGTACC AAACCCTGAC AGAGCTACAT
    ATCAGCCTGC TGCACAAAAC TGTGGTCTTC ACGTTTGCCC CAGAGCTGGA GGAGCAGGAG
    GGGATCCAGA AGAGGGCTAC GTTTCCTGAC AGCCTTATGT TCCAGATGTC CTCTGAGCCA
    TCTCTGCGCT CCCCGAGGAT CAGGCTGACG CACTGTGCCC TGCAAGCCCA GCGCCTGGAG
    CTGCTCTGCA AAGCGCTGGG AGGAAGTTGC CGCCTCAGTC ACCTTGACTT ATCAGGCAAT
    GCTCTGGGGG ATGAAGGTGT GGCCCTGCTG GCTCGGCTGC TCCCCGGACT GGGTTCTCTG
    CAGTCCTTGA ACCTCAGTGA GAACCATTTG TCCCTGGAGG GTGTGTGCAG TTTGACCCAG
    TGCTTCTCTA CTCTGCGGTG GCTTCTACAT TTGGAATTCA GCTCTGCAAG CCAACACGTC
    ATCCTGAGAG GCGACAAAAG AGGCAGGGAT CTGTTGGCTG CTGGATCTTT GCCAGAGTTC
    CGAGCTGGAG CCCGGTTGGG ATCTGGTCAG CGCCACGTCC CCAGGAGCTT CTGCCTCAAG
    GAATGTCAGC TGGAGCCCCT GAGCCTCTCC TACCTCTGCA AGTCTCTGGA GAAGTGCCCA
    GGGCCCCTGG AAGTCCAATT GTCCTGCGAG GTGCTGAGTG ACCAGAGCCT GGAGACCCTG
    CTGTACCACC TACCGCAGCT CCCCCAGCTA AGCCTGCTGC AACTGAGCCA GATGCAACTG
    CCTCCAGAGA GCCCCCTCCT GCTAGCTGAC CTCTTCAGCC TGTGCCCACA GGTTCAGAAG
    GTGGATCTCA GGTCCCTGCG TCATTTGACC CTGCATCTCA GGTCTAGTGA GGAGCAGGAA
    GACAGGTGCT GTTGGTTCAC TGACTGCGGC CTCCGCCAGG AGCACGTGGA GCCGCTGTGC
    GGGTTGCTGA GCAAGTGTGA AGACCTCCAC CAGCTGGACC TCTCAGCAAA CCTGCTGGGT
    GATGATGGGC TCAGGTGCCT CCTGGAATGT CTCCCCAAGG TGCCCATTTC CGGTTCGCTT
    GATCTAAGCC ACAACAGTAT GTCTCAGGAA AGCGTCTTGT GCCTGGTGGA GGCGCTCCCC
    TCCTGCCCAC GTCTCCGGGA GGCCTCAGTG AACCTGGGCT CCAAGCAGAG CTTCTGGATT
    CACTTCTCCC GACAGGAAGA GACTGGGAAG ACACTGAGGC TGAGCGAGTG CCACTTCAAG
    CCAGAGCACA TGCCCAGACT GGCCGCCAGC CTGAGCCAGG CCCTGCAGCT GACGGAGCTC
    ACGCTGACCT GGTGTTTCCC GGGCCTGGAG CAGCTGACTG TCCTCCTGGG TCTGCTAACG
    TGGCCGACGG GGGTGCTCAC TCTCAGGGTG GAAGAGCCAT GGGTGGGCAG AGTCGGGGTG
    CTCACGCTGC TGGAAGTCTG TGCCCAGGCC TCAGGCAACA TCACTGGAAT AAGCATCTCC
    GAGACCCAGA AACAGCTCTG TATCCAGCTG GAATTTCCCC ATCAGGAGAA CCCAGAAGCC
    GTGGCCCTCA GGCTGGCTCA CTGTGACCTG GGAACCCAAC AAAACCTCCT CGCCAGGAAG
    TTGATGGAGA CATGTGCCCG GCTGCGGCAG CTCAGTTTGT CCCAGGTGAA CCTCTGCGAT
    GCCAGCTCTC TGCTGCTGCA AAGCCTCCTG CTGTCCCTCT CTGAGCTGAA GAACTTCAGG
    CTGACCTCCA GCTGTGTGAG CTCCAAGGGG CTAGCCCTCC TGACATCTGG TCTGAGCCAT
    TGTCACCTTC TGGAGGAGCT AGACTTGTCT AACAATCAAC TTGGAGACGA GGGCACCAAG
    GTGTTGTTGG GGGCTCTTGA GGGCAAGTGC AGGCTGAAGA GACTTGACCT CAGCCACCTG
    CCACTGGGCG GCTCCACCCT GGCTGTGCTC ACTCAAGGAC TAGGCCACAT GACACTCCTG
    CAGAGCCTCC GTCTGAGCAA GAGCAACATC GATGACGTCG GCTGCTGCCA CCTTTCGAAG
    GCTCTCAGAG CTGCCACCAG CTTGGAGGAG CTGAGCTTGA GCCACAACCA GATCGGAAAC
    ATCGGTGCCC AGGACTTAGC TGCTGTCCTG CCGGGGCTCC CTGAGCTCAG GAAGATAGAC
    CTCTCAGCAA ATGGCATCGG CCCAGCAGGC GGAGCGCGGT TGGCAGAATC TCTCACCCTT
    TGCAGGCACC TCGAGGAGCT GATGCTTGGC TGCAATGCCC TGGGAGACTG CACAGCCCTG
    GGTCTGGCCA AGGGGCTGCC CCAGCACCTG AGGGTCCTGC ACCTGCCATC CAGCCATCTG
    AGCCCAGAGG GGGTGCTGAG TCTGGGCCAG GCCCTGGATG GATGCCCACA CGTGGAAGAG
    ATCAGCTTGG CAGAGAACAG CCTGGCTGGA GGGGTCCCGC ATTTCCATCA GGGGTTCCCA
    GTGCTCCGGA AGCTTGACCT GGTGTCGTGT GAGATTGACA ACCAGACCGC CAAGCCCCTC
    GCCGCCAGCT TCGTGCTCTG TCTGGCCCTG GAAGAAATCA CGCTGTCCTG GAATCTGCTC
    GGGGATGAGG CAGCTGCTGA GCTGGCCCGG GTCCTGCCGC AGATGGGCCG GCTGAAGAGA
    ATGGACCTGG AGAAAAATCA GATCACAGCT TATGGAGCCT GGCTCCTGGC TGAAGGGCTG
    GCACAGGGAT CTGGCATCCA AGTCATTCGC CTCTGGAATA ACCGTATTCC CCCGGACATG
    GCCCAGCATC TGCAGAACCA GGAGCCCAGG CTGGACTTTG CTTTCTTTGA CAACCAGCCA
    CAGGTCATCC CTCGGGGTAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09294
    Clone ID Related Accession (Same CDS sequence) XM_005218805.3 , XM_005195224.3 , XM_005218805.4 , XM_024978951.1 , XM_024978950.1
    Accession Version XM_024978951.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein NLRC5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG ACCTCTCAGG GAACCAGCTC AAAGATAAAG GCTGTCAACT GATGGCAGAG
    GCTGCTCCCC AACTGCACAT CACGAAGAAG CTGGACCTCA GCGACAATGA GCTTTCTGTG
    GCCGGGGTGC TGGGAGTACT GAGTGTGGTG AACACGTACC AAACCCTGAC AGAGCTACAT
    ATCAGCCTGC TGCACAAAAC TGTGGTCTTC ACGTTTGCCC CAGAGCTGGA GGAGCAGGAG
    GGGATCCAGA AGAGGGCTAC GTTTCCTGAC AGCCTTATGT TCCAGATGTC CTCTGAGCCA
    TCTCTGCGCT CCCCGAGGAT CAGGCTGACG CACTGTGCCC TGCAAGCCCA GCGCCTGGAG
    CTGCTCTGCA AAGCGCTGGG AGGAAGTTGC CGCCTCAGTC ACCTTGACTT ATCAGGCAAT
    GCTCTGGGGG ATGAAGGTGT GGCCCTGCTG GCTCGGCTGC TCCCCGGACT GGGTTCTCTG
    CAGTCCTTGA ACCTCAGTGA GAACCATTTG TCCCTGGAGG GTGTGTGCAG TTTGACCCAG
    TGCTTCTCTA CTCTGCGGTG GCTTCTACAT TTGGAATTCA GCTCTGCAAG CCAACACGTC
    ATCCTGAGAG GCGACAAAAG AGGCAGGGAT CTGTTGGCTG CTGGATCTTT GCCAGAGTTC
    CGAGCTGGAG CCCGGTTGGG ATCTGGTCAG CGCCACGTCC CCAGGAGCTT CTGCCTCAAG
    GAATGTCAGC TGGAGCCCCT GAGCCTCTCC TACCTCTGCA AGTCTCTGGA GAAGTGCCCA
    GGGCCCCTGG AAGTCCAATT GTCCTGCGAG GTGCTGAGTG ACCAGAGCCT GGAGACCCTG
    CTGTACCACC TACCGCAGCT CCCCCAGCTA AGCCTGCTGC AACTGAGCCA GATGCAACTG
    CCTCCAGAGA GCCCCCTCCT GCTAGCTGAC CTCTTCAGCC TGTGCCCACA GGTTCAGAAG
    GTGGATCTCA GGTCCCTGCG TCATTTGACC CTGCATCTCA GGTCTAGTGA GGAGCAGGAA
    GACAGGTGCT GTTGGTTCAC TGACTGCGGC CTCCGCCAGG AGCACGTGGA GCCGCTGTGC
    GGGTTGCTGA GCAAGTGTGA AGACCTCCAC CAGCTGGACC TCTCAGCAAA CCTGCTGGGT
    GATGATGGGC TCAGGTGCCT CCTGGAATGT CTCCCCAAGG TGCCCATTTC CGGTTCGCTT
    GATCTAAGCC ACAACAGTAT GTCTCAGGAA AGCGTCTTGT GCCTGGTGGA GGCGCTCCCC
    TCCTGCCCAC GTCTCCGGGA GGCCTCAGTG AACCTGGGCT CCAAGCAGAG CTTCTGGATT
    CACTTCTCCC GACAGGAAGA GACTGGGAAG ACACTGAGGC TGAGCGAGTG CCACTTCAAG
    CCAGAGCACA TGCCCAGACT GGCCGCCAGC CTGAGCCAGG CCCTGCAGCT GACGGAGCTC
    ACGCTGACCT GGTGTTTCCC GGGCCTGGAG CAGCTGACTG TCCTCCTGGG TCTGCTAACG
    TGGCCGACGG GGGTGCTCAC TCTCAGGGTG GAAGAGCCAT GGGTGGGCAG AGTCGGGGTG
    CTCACGCTGC TGGAAGTCTG TGCCCAGGCC TCAGGCAACA TCACTGGAAT AAGCATCTCC
    GAGACCCAGA AACAGCTCTG TATCCAGCTG GAATTTCCCC ATCAGGAGAA CCCAGAAGCC
    GTGGCCCTCA GGCTGGCTCA CTGTGACCTG GGAACCCAAC AAAACCTCCT CGCCAGGAAG
    TTGATGGAGA CATGTGCCCG GCTGCGGCAG CTCAGTTTGT CCCAGGTGAA CCTCTGCGAT
    GCCAGCTCTC TGCTGCTGCA AAGCCTCCTG CTGTCCCTCT CTGAGCTGAA GAACTTCAGG
    CTGACCTCCA GCTGTGTGAG CTCCAAGGGG CTAGCCCTCC TGACATCTGG TCTGAGCCAT
    TGTCACCTTC TGGAGGAGCT AGACTTGTCT AACAATCAAC TTGGAGACGA GGGCACCAAG
    GTGTTGTTGG GGGCTCTTGA GGGCAAGTGC AGGCTGAAGA GACTTGACCT CAGCCACCTG
    CCACTGGGCG GCTCCACCCT GGCTGTGCTC ACTCAAGGAC TAGGCCACAT GACACTCCTG
    CAGAGCCTCC GTCTGAGCAA GAGCAACATC GATGACGTCG GCTGCTGCCA CCTTTCGAAG
    GCTCTCAGAG CTGCCACCAG CTTGGAGGAG CTGAGCTTGA GCCACAACCA GATCGGAAAC
    ATCGGTGCCC AGGACTTAGC TGCTGTCCTG CCGGGGCTCC CTGAGCTCAG GAAGATAGAC
    CTCTCAGCAA ATGGCATCGG CCCAGCAGGC GGAGCGCGGT TGGCAGAATC TCTCACCCTT
    TGCAGGCACC TCGAGGAGCT GATGCTTGGC TGCAATGCCC TGGGAGACTG CACAGCCCTG
    GGTCTGGCCA AGGGGCTGCC CCAGCACCTG AGGGTCCTGC ACCTGCCATC CAGCCATCTG
    AGCCCAGAGG GGGTGCTGAG TCTGGGCCAG GCCCTGGATG GATGCCCACA CGTGGAAGAG
    ATCAGCTTGG CAGAGAACAG CCTGGCTGGA GGGGTCCCGC ATTTCCATCA GGGGTTCCCA
    GTGCTCCGGA AGCTTGACCT GGTGTCGTGT GAGATTGACA ACCAGACCGC CAAGCCCCTC
    GCCGCCAGCT TCGTGCTCTG TCTGGCCCTG GAAGAAATCA CGCTGTCCTG GAATCTGCTC
    GGGGATGAGG CAGCTGCTGA GCTGGCCCGG GTCCTGCCGC AGATGGGCCG GCTGAAGAGA
    ATGGACCTGG AGAAAAATCA GATCACAGCT TATGGAGCCT GGCTCCTGGC TGAAGGGCTG
    GCACAGGGAT CTGGCATCCA AGTCATTCGC CTCTGGAATA ACCGTATTCC CCCGGACATG
    GCCCAGCATC TGCAGAACCA GGAGCCCAGG CTGGACTTTG CTTTCTTTGA CAACCAGCCA
    CAGGTCATCC CTCGGGGTAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834719.1
    CDS596..6199
    Translation

    Target ORF information:

    RefSeq Version XM_024978951.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family CARD domain containing 5 (NLRC5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978951.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG ACCTCTCAGG GAACCAGCTC AAAGATAAAG GCTGTCAACT GATGGCAGAG
    GCTGCTCCCC AACTGCACAT CACGAAGAAG CTGGACCTCA GCGACAATGA GCTTTCTGTG
    GCCGGGGTGC TGGGAGTACT GAGTGTGGTG AACACGTACC AAACCCTGAC AGAGCTACAT
    ATCAGCCTGC TGCACAAAAC TGTGGTCTTC ACGTTTGCCC CAGAGCTGGA GGAGCAGGAG
    GGGATCCAGA AGAGGGCTAC GTTTCCTGAC AGCCTTATGT TCCAGATGTC CTCTGAGCCA
    TCTCTGCGCT CCCCGAGGAT CAGGCTGACG CACTGTGCCC TGCAAGCCCA GCGCCTGGAG
    CTGCTCTGCA AAGCGCTGGG AGGAAGTTGC CGCCTCAGTC ACCTTGACTT ATCAGGCAAT
    GCTCTGGGGG ATGAAGGTGT GGCCCTGCTG GCTCGGCTGC TCCCCGGACT GGGTTCTCTG
    CAGTCCTTGA ACCTCAGTGA GAACCATTTG TCCCTGGAGG GTGTGTGCAG TTTGACCCAG
    TGCTTCTCTA CTCTGCGGTG GCTTCTACAT TTGGAATTCA GCTCTGCAAG CCAACACGTC
    ATCCTGAGAG GCGACAAAAG AGGCAGGGAT CTGTTGGCTG CTGGATCTTT GCCAGAGTTC
    CGAGCTGGAG CCCGGTTGGG ATCTGGTCAG CGCCACGTCC CCAGGAGCTT CTGCCTCAAG
    GAATGTCAGC TGGAGCCCCT GAGCCTCTCC TACCTCTGCA AGTCTCTGGA GAAGTGCCCA
    GGGCCCCTGG AAGTCCAATT GTCCTGCGAG GTGCTGAGTG ACCAGAGCCT GGAGACCCTG
    CTGTACCACC TACCGCAGCT CCCCCAGCTA AGCCTGCTGC AACTGAGCCA GATGCAACTG
    CCTCCAGAGA GCCCCCTCCT GCTAGCTGAC CTCTTCAGCC TGTGCCCACA GGTTCAGAAG
    GTGGATCTCA GGTCCCTGCG TCATTTGACC CTGCATCTCA GGTCTAGTGA GGAGCAGGAA
    GACAGGTGCT GTTGGTTCAC TGACTGCGGC CTCCGCCAGG AGCACGTGGA GCCGCTGTGC
    GGGTTGCTGA GCAAGTGTGA AGACCTCCAC CAGCTGGACC TCTCAGCAAA CCTGCTGGGT
    GATGATGGGC TCAGGTGCCT CCTGGAATGT CTCCCCAAGG TGCCCATTTC CGGTTCGCTT
    GATCTAAGCC ACAACAGTAT GTCTCAGGAA AGCGTCTTGT GCCTGGTGGA GGCGCTCCCC
    TCCTGCCCAC GTCTCCGGGA GGCCTCAGTG AACCTGGGCT CCAAGCAGAG CTTCTGGATT
    CACTTCTCCC GACAGGAAGA GACTGGGAAG ACACTGAGGC TGAGCGAGTG CCACTTCAAG
    CCAGAGCACA TGCCCAGACT GGCCGCCAGC CTGAGCCAGG CCCTGCAGCT GACGGAGCTC
    ACGCTGACCT GGTGTTTCCC GGGCCTGGAG CAGCTGACTG TCCTCCTGGG TCTGCTAACG
    TGGCCGACGG GGGTGCTCAC TCTCAGGGTG GAAGAGCCAT GGGTGGGCAG AGTCGGGGTG
    CTCACGCTGC TGGAAGTCTG TGCCCAGGCC TCAGGCAACA TCACTGGAAT AAGCATCTCC
    GAGACCCAGA AACAGCTCTG TATCCAGCTG GAATTTCCCC ATCAGGAGAA CCCAGAAGCC
    GTGGCCCTCA GGCTGGCTCA CTGTGACCTG GGAACCCAAC AAAACCTCCT CGCCAGGAAG
    TTGATGGAGA CATGTGCCCG GCTGCGGCAG CTCAGTTTGT CCCAGGTGAA CCTCTGCGAT
    GCCAGCTCTC TGCTGCTGCA AAGCCTCCTG CTGTCCCTCT CTGAGCTGAA GAACTTCAGG
    CTGACCTCCA GCTGTGTGAG CTCCAAGGGG CTAGCCCTCC TGACATCTGG TCTGAGCCAT
    TGTCACCTTC TGGAGGAGCT AGACTTGTCT AACAATCAAC TTGGAGACGA GGGCACCAAG
    GTGTTGTTGG GGGCTCTTGA GGGCAAGTGC AGGCTGAAGA GACTTGACCT CAGCCACCTG
    CCACTGGGCG GCTCCACCCT GGCTGTGCTC ACTCAAGGAC TAGGCCACAT GACACTCCTG
    CAGAGCCTCC GTCTGAGCAA GAGCAACATC GATGACGTCG GCTGCTGCCA CCTTTCGAAG
    GCTCTCAGAG CTGCCACCAG CTTGGAGGAG CTGAGCTTGA GCCACAACCA GATCGGAAAC
    ATCGGTGCCC AGGACTTAGC TGCTGTCCTG CCGGGGCTCC CTGAGCTCAG GAAGATAGAC
    CTCTCAGCAA ATGGCATCGG CCCAGCAGGC GGAGCGCGGT TGGCAGAATC TCTCACCCTT
    TGCAGGCACC TCGAGGAGCT GATGCTTGGC TGCAATGCCC TGGGAGACTG CACAGCCCTG
    GGTCTGGCCA AGGGGCTGCC CCAGCACCTG AGGGTCCTGC ACCTGCCATC CAGCCATCTG
    AGCCCAGAGG GGGTGCTGAG TCTGGGCCAG GCCCTGGATG GATGCCCACA CGTGGAAGAG
    ATCAGCTTGG CAGAGAACAG CCTGGCTGGA GGGGTCCCGC ATTTCCATCA GGGGTTCCCA
    GTGCTCCGGA AGCTTGACCT GGTGTCGTGT GAGATTGACA ACCAGACCGC CAAGCCCCTC
    GCCGCCAGCT TCGTGCTCTG TCTGGCCCTG GAAGAAATCA CGCTGTCCTG GAATCTGCTC
    GGGGATGAGG CAGCTGCTGA GCTGGCCCGG GTCCTGCCGC AGATGGGCCG GCTGAAGAGA
    ATGGACCTGG AGAAAAATCA GATCACAGCT TATGGAGCCT GGCTCCTGGC TGAAGGGCTG
    GCACAGGGAT CTGGCATCCA AGTCATTCGC CTCTGGAATA ACCGTATTCC CCCGGACATG
    GCCCAGCATC TGCAGAACCA GGAGCCCAGG CTGGACTTTG CTTTCTTTGA CAACCAGCCA
    CAGGTCATCC CTCGGGGTAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09294
    Clone ID Related Accession (Same CDS sequence) XM_005218805.3 , XM_005195224.3 , XM_005218805.4 , XM_024978951.1 , XM_024978950.1
    Accession Version XM_024978950.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein NLRC5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG ACCTCTCAGG GAACCAGCTC AAAGATAAAG GCTGTCAACT GATGGCAGAG
    GCTGCTCCCC AACTGCACAT CACGAAGAAG CTGGACCTCA GCGACAATGA GCTTTCTGTG
    GCCGGGGTGC TGGGAGTACT GAGTGTGGTG AACACGTACC AAACCCTGAC AGAGCTACAT
    ATCAGCCTGC TGCACAAAAC TGTGGTCTTC ACGTTTGCCC CAGAGCTGGA GGAGCAGGAG
    GGGATCCAGA AGAGGGCTAC GTTTCCTGAC AGCCTTATGT TCCAGATGTC CTCTGAGCCA
    TCTCTGCGCT CCCCGAGGAT CAGGCTGACG CACTGTGCCC TGCAAGCCCA GCGCCTGGAG
    CTGCTCTGCA AAGCGCTGGG AGGAAGTTGC CGCCTCAGTC ACCTTGACTT ATCAGGCAAT
    GCTCTGGGGG ATGAAGGTGT GGCCCTGCTG GCTCGGCTGC TCCCCGGACT GGGTTCTCTG
    CAGTCCTTGA ACCTCAGTGA GAACCATTTG TCCCTGGAGG GTGTGTGCAG TTTGACCCAG
    TGCTTCTCTA CTCTGCGGTG GCTTCTACAT TTGGAATTCA GCTCTGCAAG CCAACACGTC
    ATCCTGAGAG GCGACAAAAG AGGCAGGGAT CTGTTGGCTG CTGGATCTTT GCCAGAGTTC
    CGAGCTGGAG CCCGGTTGGG ATCTGGTCAG CGCCACGTCC CCAGGAGCTT CTGCCTCAAG
    GAATGTCAGC TGGAGCCCCT GAGCCTCTCC TACCTCTGCA AGTCTCTGGA GAAGTGCCCA
    GGGCCCCTGG AAGTCCAATT GTCCTGCGAG GTGCTGAGTG ACCAGAGCCT GGAGACCCTG
    CTGTACCACC TACCGCAGCT CCCCCAGCTA AGCCTGCTGC AACTGAGCCA GATGCAACTG
    CCTCCAGAGA GCCCCCTCCT GCTAGCTGAC CTCTTCAGCC TGTGCCCACA GGTTCAGAAG
    GTGGATCTCA GGTCCCTGCG TCATTTGACC CTGCATCTCA GGTCTAGTGA GGAGCAGGAA
    GACAGGTGCT GTTGGTTCAC TGACTGCGGC CTCCGCCAGG AGCACGTGGA GCCGCTGTGC
    GGGTTGCTGA GCAAGTGTGA AGACCTCCAC CAGCTGGACC TCTCAGCAAA CCTGCTGGGT
    GATGATGGGC TCAGGTGCCT CCTGGAATGT CTCCCCAAGG TGCCCATTTC CGGTTCGCTT
    GATCTAAGCC ACAACAGTAT GTCTCAGGAA AGCGTCTTGT GCCTGGTGGA GGCGCTCCCC
    TCCTGCCCAC GTCTCCGGGA GGCCTCAGTG AACCTGGGCT CCAAGCAGAG CTTCTGGATT
    CACTTCTCCC GACAGGAAGA GACTGGGAAG ACACTGAGGC TGAGCGAGTG CCACTTCAAG
    CCAGAGCACA TGCCCAGACT GGCCGCCAGC CTGAGCCAGG CCCTGCAGCT GACGGAGCTC
    ACGCTGACCT GGTGTTTCCC GGGCCTGGAG CAGCTGACTG TCCTCCTGGG TCTGCTAACG
    TGGCCGACGG GGGTGCTCAC TCTCAGGGTG GAAGAGCCAT GGGTGGGCAG AGTCGGGGTG
    CTCACGCTGC TGGAAGTCTG TGCCCAGGCC TCAGGCAACA TCACTGGAAT AAGCATCTCC
    GAGACCCAGA AACAGCTCTG TATCCAGCTG GAATTTCCCC ATCAGGAGAA CCCAGAAGCC
    GTGGCCCTCA GGCTGGCTCA CTGTGACCTG GGAACCCAAC AAAACCTCCT CGCCAGGAAG
    TTGATGGAGA CATGTGCCCG GCTGCGGCAG CTCAGTTTGT CCCAGGTGAA CCTCTGCGAT
    GCCAGCTCTC TGCTGCTGCA AAGCCTCCTG CTGTCCCTCT CTGAGCTGAA GAACTTCAGG
    CTGACCTCCA GCTGTGTGAG CTCCAAGGGG CTAGCCCTCC TGACATCTGG TCTGAGCCAT
    TGTCACCTTC TGGAGGAGCT AGACTTGTCT AACAATCAAC TTGGAGACGA GGGCACCAAG
    GTGTTGTTGG GGGCTCTTGA GGGCAAGTGC AGGCTGAAGA GACTTGACCT CAGCCACCTG
    CCACTGGGCG GCTCCACCCT GGCTGTGCTC ACTCAAGGAC TAGGCCACAT GACACTCCTG
    CAGAGCCTCC GTCTGAGCAA GAGCAACATC GATGACGTCG GCTGCTGCCA CCTTTCGAAG
    GCTCTCAGAG CTGCCACCAG CTTGGAGGAG CTGAGCTTGA GCCACAACCA GATCGGAAAC
    ATCGGTGCCC AGGACTTAGC TGCTGTCCTG CCGGGGCTCC CTGAGCTCAG GAAGATAGAC
    CTCTCAGCAA ATGGCATCGG CCCAGCAGGC GGAGCGCGGT TGGCAGAATC TCTCACCCTT
    TGCAGGCACC TCGAGGAGCT GATGCTTGGC TGCAATGCCC TGGGAGACTG CACAGCCCTG
    GGTCTGGCCA AGGGGCTGCC CCAGCACCTG AGGGTCCTGC ACCTGCCATC CAGCCATCTG
    AGCCCAGAGG GGGTGCTGAG TCTGGGCCAG GCCCTGGATG GATGCCCACA CGTGGAAGAG
    ATCAGCTTGG CAGAGAACAG CCTGGCTGGA GGGGTCCCGC ATTTCCATCA GGGGTTCCCA
    GTGCTCCGGA AGCTTGACCT GGTGTCGTGT GAGATTGACA ACCAGACCGC CAAGCCCCTC
    GCCGCCAGCT TCGTGCTCTG TCTGGCCCTG GAAGAAATCA CGCTGTCCTG GAATCTGCTC
    GGGGATGAGG CAGCTGCTGA GCTGGCCCGG GTCCTGCCGC AGATGGGCCG GCTGAAGAGA
    ATGGACCTGG AGAAAAATCA GATCACAGCT TATGGAGCCT GGCTCCTGGC TGAAGGGCTG
    GCACAGGGAT CTGGCATCCA AGTCATTCGC CTCTGGAATA ACCGTATTCC CCCGGACATG
    GCCCAGCATC TGCAGAACCA GGAGCCCAGG CTGGACTTTG CTTTCTTTGA CAACCAGCCA
    CAGGTCATCC CTCGGGGTAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834718.1
    CDS558..6161
    Translation

    Target ORF information:

    RefSeq Version XM_024978950.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family CARD domain containing 5 (NLRC5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978950.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG ACCTCTCAGG GAACCAGCTC AAAGATAAAG GCTGTCAACT GATGGCAGAG
    GCTGCTCCCC AACTGCACAT CACGAAGAAG CTGGACCTCA GCGACAATGA GCTTTCTGTG
    GCCGGGGTGC TGGGAGTACT GAGTGTGGTG AACACGTACC AAACCCTGAC AGAGCTACAT
    ATCAGCCTGC TGCACAAAAC TGTGGTCTTC ACGTTTGCCC CAGAGCTGGA GGAGCAGGAG
    GGGATCCAGA AGAGGGCTAC GTTTCCTGAC AGCCTTATGT TCCAGATGTC CTCTGAGCCA
    TCTCTGCGCT CCCCGAGGAT CAGGCTGACG CACTGTGCCC TGCAAGCCCA GCGCCTGGAG
    CTGCTCTGCA AAGCGCTGGG AGGAAGTTGC CGCCTCAGTC ACCTTGACTT ATCAGGCAAT
    GCTCTGGGGG ATGAAGGTGT GGCCCTGCTG GCTCGGCTGC TCCCCGGACT GGGTTCTCTG
    CAGTCCTTGA ACCTCAGTGA GAACCATTTG TCCCTGGAGG GTGTGTGCAG TTTGACCCAG
    TGCTTCTCTA CTCTGCGGTG GCTTCTACAT TTGGAATTCA GCTCTGCAAG CCAACACGTC
    ATCCTGAGAG GCGACAAAAG AGGCAGGGAT CTGTTGGCTG CTGGATCTTT GCCAGAGTTC
    CGAGCTGGAG CCCGGTTGGG ATCTGGTCAG CGCCACGTCC CCAGGAGCTT CTGCCTCAAG
    GAATGTCAGC TGGAGCCCCT GAGCCTCTCC TACCTCTGCA AGTCTCTGGA GAAGTGCCCA
    GGGCCCCTGG AAGTCCAATT GTCCTGCGAG GTGCTGAGTG ACCAGAGCCT GGAGACCCTG
    CTGTACCACC TACCGCAGCT CCCCCAGCTA AGCCTGCTGC AACTGAGCCA GATGCAACTG
    CCTCCAGAGA GCCCCCTCCT GCTAGCTGAC CTCTTCAGCC TGTGCCCACA GGTTCAGAAG
    GTGGATCTCA GGTCCCTGCG TCATTTGACC CTGCATCTCA GGTCTAGTGA GGAGCAGGAA
    GACAGGTGCT GTTGGTTCAC TGACTGCGGC CTCCGCCAGG AGCACGTGGA GCCGCTGTGC
    GGGTTGCTGA GCAAGTGTGA AGACCTCCAC CAGCTGGACC TCTCAGCAAA CCTGCTGGGT
    GATGATGGGC TCAGGTGCCT CCTGGAATGT CTCCCCAAGG TGCCCATTTC CGGTTCGCTT
    GATCTAAGCC ACAACAGTAT GTCTCAGGAA AGCGTCTTGT GCCTGGTGGA GGCGCTCCCC
    TCCTGCCCAC GTCTCCGGGA GGCCTCAGTG AACCTGGGCT CCAAGCAGAG CTTCTGGATT
    CACTTCTCCC GACAGGAAGA GACTGGGAAG ACACTGAGGC TGAGCGAGTG CCACTTCAAG
    CCAGAGCACA TGCCCAGACT GGCCGCCAGC CTGAGCCAGG CCCTGCAGCT GACGGAGCTC
    ACGCTGACCT GGTGTTTCCC GGGCCTGGAG CAGCTGACTG TCCTCCTGGG TCTGCTAACG
    TGGCCGACGG GGGTGCTCAC TCTCAGGGTG GAAGAGCCAT GGGTGGGCAG AGTCGGGGTG
    CTCACGCTGC TGGAAGTCTG TGCCCAGGCC TCAGGCAACA TCACTGGAAT AAGCATCTCC
    GAGACCCAGA AACAGCTCTG TATCCAGCTG GAATTTCCCC ATCAGGAGAA CCCAGAAGCC
    GTGGCCCTCA GGCTGGCTCA CTGTGACCTG GGAACCCAAC AAAACCTCCT CGCCAGGAAG
    TTGATGGAGA CATGTGCCCG GCTGCGGCAG CTCAGTTTGT CCCAGGTGAA CCTCTGCGAT
    GCCAGCTCTC TGCTGCTGCA AAGCCTCCTG CTGTCCCTCT CTGAGCTGAA GAACTTCAGG
    CTGACCTCCA GCTGTGTGAG CTCCAAGGGG CTAGCCCTCC TGACATCTGG TCTGAGCCAT
    TGTCACCTTC TGGAGGAGCT AGACTTGTCT AACAATCAAC TTGGAGACGA GGGCACCAAG
    GTGTTGTTGG GGGCTCTTGA GGGCAAGTGC AGGCTGAAGA GACTTGACCT CAGCCACCTG
    CCACTGGGCG GCTCCACCCT GGCTGTGCTC ACTCAAGGAC TAGGCCACAT GACACTCCTG
    CAGAGCCTCC GTCTGAGCAA GAGCAACATC GATGACGTCG GCTGCTGCCA CCTTTCGAAG
    GCTCTCAGAG CTGCCACCAG CTTGGAGGAG CTGAGCTTGA GCCACAACCA GATCGGAAAC
    ATCGGTGCCC AGGACTTAGC TGCTGTCCTG CCGGGGCTCC CTGAGCTCAG GAAGATAGAC
    CTCTCAGCAA ATGGCATCGG CCCAGCAGGC GGAGCGCGGT TGGCAGAATC TCTCACCCTT
    TGCAGGCACC TCGAGGAGCT GATGCTTGGC TGCAATGCCC TGGGAGACTG CACAGCCCTG
    GGTCTGGCCA AGGGGCTGCC CCAGCACCTG AGGGTCCTGC ACCTGCCATC CAGCCATCTG
    AGCCCAGAGG GGGTGCTGAG TCTGGGCCAG GCCCTGGATG GATGCCCACA CGTGGAAGAG
    ATCAGCTTGG CAGAGAACAG CCTGGCTGGA GGGGTCCCGC ATTTCCATCA GGGGTTCCCA
    GTGCTCCGGA AGCTTGACCT GGTGTCGTGT GAGATTGACA ACCAGACCGC CAAGCCCCTC
    GCCGCCAGCT TCGTGCTCTG TCTGGCCCTG GAAGAAATCA CGCTGTCCTG GAATCTGCTC
    GGGGATGAGG CAGCTGCTGA GCTGGCCCGG GTCCTGCCGC AGATGGGCCG GCTGAAGAGA
    ATGGACCTGG AGAAAAATCA GATCACAGCT TATGGAGCCT GGCTCCTGGC TGAAGGGCTG
    GCACAGGGAT CTGGCATCCA AGTCATTCGC CTCTGGAATA ACCGTATTCC CCCGGACATG
    GCCCAGCATC TGCAGAACCA GGAGCCCAGG CTGGACTTTG CTTTCTTTGA CAACCAGCCA
    CAGGTCATCC CTCGGGGTAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57241
    Clone ID Related Accession (Same CDS sequence) XM_010823574.2 , XM_010814628.2
    Accession Version XM_010823574.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein NLRC5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823574.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG ACCTCTCAGG GAACCAGCTC AAAGATAAAG GCTGTCAACT GATGGCAGAG
    GCTGCTCCCC AACTGCACAT CACGAAGAAG CTGGACCTCA GCGACAATGA GCTTTCTGTG
    GCCGGGGTGC TGGGAGTACT GAGTGTGGTG AACACGTACC AAACCCTGAC AGAGCTACAT
    ATCAGCCTGC TGCACAAAAC TGTGGTCTTC ACGTTTGCCC CAGAGCTGGA GGAGCAGGAG
    GGGATCCAGA AGAGGGCTAC GTTTCCTGAC AGCCTTATGT TCCAGATGTC CTCTGAGCCA
    TCTCTGCGCT CCCCGAGGAT CAGGCTGACG CACTGTGCCC TGCAAGCCCA GCGCCTGGAG
    CTGCTCTGCA AAGCGCTGGG AGGAAGTTGC CGCCTCAGTC ACCTTGACTT ATCAGGCAAT
    GCTCTGGGGG ATGAAGGTGT GGCCCTGCTG GCTCGGCTGC TCCCCGGACT GGGTTCTCTG
    CAGTCCTTGA ACCTCAGTGA GAACCATTTG TCCCTGGAGG GTGTGTGCAG TTTGACCCAG
    TGCTTCTCTA CTCTGCGGTG GCTTCTACAT TTGGAATTCA GCTCTGCAAG CCAACACGTC
    ATCCTGAGAG GCGACAAAAG AGGCAGGGAT CTGTTGGCTG CTGGATCTTT GCCAGAGTTC
    CGAGCTGGAG CCCGGTTGGG ATCTGGTCAG CGCCACGTCC CCAGGAGCTT CTGCCTCAAG
    GAATGTCAGC TGGAGCCCCT GAGCCTCTCC TACCTCTGCA AGTCTCTGGA GAAGTGCCCA
    GGGCCCCTGG AAGTCCAATT GTCCTGCGAG GTGCTGAGTG ACCAGAGCCT GGAGACCCTG
    CTGTACCACC TACCGCAGCT CCCCCAGCTA AGCCTGCTGC AACTGAGCCA GATGCAACTG
    CCTCCAGAGA GCCCCCTCCT GCTAGCTGAC CTCTTCAGCC TGTGCCCACA GGTTCAGAAG
    GTGGATCTCA GGTCCCTGCG TCATTTGACC CTGCATCTCA GGTCTAGTGA GGAGCAGGAA
    GACAGGTGCT GTTGGTTCAC TGACTGCGGC CTCCGCCAGG AGCACGTGGA GCCGCTGTGC
    GGGTTGCTGA GCAAGTGTGA AGACCTCCAC CAGCTGGACC TCTCAGCAAA CCTGCTGGGT
    GATGATGGGC TCAGGTGCCT CCTGGAATGT CTCCCCAAGG TGCCCATTTC CGGTTCGCTT
    GATCTAAGCC ACAACAGTAT GTCTCAGGAA AGCGTCTTGT GCCTGGTGGA GGCGCTCCCC
    TCCTGCCCAC GTCTCCGGGA GGCCTCAGTG AACCTGGGCT CCAAGCAGAG CTTCTGGATT
    CACTTCTCCC GACAGGAAGA GACTGGGAAG ACACTGAGGC TGAGCGAGTG CCACTTCAAG
    CCAGAGCACA TGCCCAGACT GGCCGCCAGC CTGAGCCAGG CCCTGCAGCT GACGGAGCTC
    ACGCTGACCT GGTGTTTCCC GGGCCTGGAG CAGCTGACTG TCCTCCTGGG TCTGCTAACG
    TGGCCGACGG GGGTGCTCAC TCTCAGGGTG GAAGAGCCAT GGGTGGGCAG AGTCGGGGTG
    CTCACGCTGC TGGAAGTCTG TGCCCAGGCC TCAGGCAACA TCACTGGAAT AAGCATCTCC
    GAGACCCAGA AACAGCTCTG TATCCAGCTG GAATTTCCCC ATCAGGAGAA CCCAGAAGCC
    GTGGCCCTCA GGCTGGCTCA CTGTGACCTG GGAACCCAAC AAAACCTCCT CGCCAGGAAG
    TTGATGGAGA CATGTGCCCG GCTGCGGCAG CTCAGTTTGT CCCAGGTGAA CCTCTGCGAT
    GCCAGCTCTC TGCTGCTGCA AAGCCTCCTG CTGTCCCTCT CTGAGCTGAA GAACTTCAGG
    CTGACCTCCA GCTGTGTGAG CTCCAAGGGG CTAGCCCTCC TGACATCTGG TCTGAGCCAT
    TGTCACCTTC TGGAGGAGCT AGACTTGTCT AACAATCAAC TTGGAGACGA GGGCACCAAG
    GTGTTGTTGG GGGCTCTTGA GGGCAAGTGC AGGCTGAAGA GACTTGACCT CAGCCACCTG
    CCACTGGGCG GCTCCACCCT GGCTGTGCTC ACTCAAGGAC TAGGCCACAT GACACTCCTG
    CAGAGCCTCC GTCTGAGCAA GAGCAACATC GATGACGTCG GCTGCTGCCA CCTTTCGAAG
    GCTCTCAGAG CTGCCACCAG CTTGGAGGAG CTGAGCTTGA GCCACAACCA GATCGGAAAC
    ATCGGTGCCC AGGACTTAGC TGCTGTCCTG CCGGGGCTCC CTGAGCTCAG GAAGATAGAC
    CTCTCAGCAA ATGGCATCGG CCCAGCAGGC GGAGCGCGGT TGGCAGAATC TCTCACCCTT
    TGCAGGCACC TCGAGGAGCT GATGCTTGGC TGCAATGCCC TGGGAGACTG CACAGCCCTG
    GGTCTGGCCA AGGGGCTGCC CCAGCACCTG AGGGTCCTGC ACCTGCCATC CAGCCATCTG
    AGCCCAGAGG GGGTGCTGAG TCTGGGCCAG GCCCTGGATG GATGCCCACA CGTGGAAGAG
    ATCAGCTTGG CAGAGAACAG CCTGGCTGGA GGGGTCCCGC ATTTCCATCA GGGGTTCCCA
    GTGCTCCGGA AGCTTGACCT GGTGTCGTGT GAGATTGACA ACCAGACCGC CAAGCCCCTC
    GCCGCCAGCT TCGTGCTCTG TCTGGCCCTG GAAGAAATCA CGCTGTCCTG GAATCTGCTC
    GGGGATGAGG CAGCTGCTGA GCTGGCCCGG GTCCTGCCGC AGATGGGCCG GCTGAAGAGA
    ATGGACCTCT GGAATAACCG TATTCCCCCG GACATGGCCC AGCATCTGCA GAACCAGGAG
    CCCAGGCTGG ACTTTGCTTT CTTTGACAAC CAGCCACAGG TCATCCCTCG GGGTACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821876.1
    CDS552..6071
    Translation

    Target ORF information:

    RefSeq Version XM_010823574.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, CARD domain containing 5 (NLRC5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010823574.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG ACCTCTCAGG GAACCAGCTC AAAGATAAAG GCTGTCAACT GATGGCAGAG
    GCTGCTCCCC AACTGCACAT CACGAAGAAG CTGGACCTCA GCGACAATGA GCTTTCTGTG
    GCCGGGGTGC TGGGAGTACT GAGTGTGGTG AACACGTACC AAACCCTGAC AGAGCTACAT
    ATCAGCCTGC TGCACAAAAC TGTGGTCTTC ACGTTTGCCC CAGAGCTGGA GGAGCAGGAG
    GGGATCCAGA AGAGGGCTAC GTTTCCTGAC AGCCTTATGT TCCAGATGTC CTCTGAGCCA
    TCTCTGCGCT CCCCGAGGAT CAGGCTGACG CACTGTGCCC TGCAAGCCCA GCGCCTGGAG
    CTGCTCTGCA AAGCGCTGGG AGGAAGTTGC CGCCTCAGTC ACCTTGACTT ATCAGGCAAT
    GCTCTGGGGG ATGAAGGTGT GGCCCTGCTG GCTCGGCTGC TCCCCGGACT GGGTTCTCTG
    CAGTCCTTGA ACCTCAGTGA GAACCATTTG TCCCTGGAGG GTGTGTGCAG TTTGACCCAG
    TGCTTCTCTA CTCTGCGGTG GCTTCTACAT TTGGAATTCA GCTCTGCAAG CCAACACGTC
    ATCCTGAGAG GCGACAAAAG AGGCAGGGAT CTGTTGGCTG CTGGATCTTT GCCAGAGTTC
    CGAGCTGGAG CCCGGTTGGG ATCTGGTCAG CGCCACGTCC CCAGGAGCTT CTGCCTCAAG
    GAATGTCAGC TGGAGCCCCT GAGCCTCTCC TACCTCTGCA AGTCTCTGGA GAAGTGCCCA
    GGGCCCCTGG AAGTCCAATT GTCCTGCGAG GTGCTGAGTG ACCAGAGCCT GGAGACCCTG
    CTGTACCACC TACCGCAGCT CCCCCAGCTA AGCCTGCTGC AACTGAGCCA GATGCAACTG
    CCTCCAGAGA GCCCCCTCCT GCTAGCTGAC CTCTTCAGCC TGTGCCCACA GGTTCAGAAG
    GTGGATCTCA GGTCCCTGCG TCATTTGACC CTGCATCTCA GGTCTAGTGA GGAGCAGGAA
    GACAGGTGCT GTTGGTTCAC TGACTGCGGC CTCCGCCAGG AGCACGTGGA GCCGCTGTGC
    GGGTTGCTGA GCAAGTGTGA AGACCTCCAC CAGCTGGACC TCTCAGCAAA CCTGCTGGGT
    GATGATGGGC TCAGGTGCCT CCTGGAATGT CTCCCCAAGG TGCCCATTTC CGGTTCGCTT
    GATCTAAGCC ACAACAGTAT GTCTCAGGAA AGCGTCTTGT GCCTGGTGGA GGCGCTCCCC
    TCCTGCCCAC GTCTCCGGGA GGCCTCAGTG AACCTGGGCT CCAAGCAGAG CTTCTGGATT
    CACTTCTCCC GACAGGAAGA GACTGGGAAG ACACTGAGGC TGAGCGAGTG CCACTTCAAG
    CCAGAGCACA TGCCCAGACT GGCCGCCAGC CTGAGCCAGG CCCTGCAGCT GACGGAGCTC
    ACGCTGACCT GGTGTTTCCC GGGCCTGGAG CAGCTGACTG TCCTCCTGGG TCTGCTAACG
    TGGCCGACGG GGGTGCTCAC TCTCAGGGTG GAAGAGCCAT GGGTGGGCAG AGTCGGGGTG
    CTCACGCTGC TGGAAGTCTG TGCCCAGGCC TCAGGCAACA TCACTGGAAT AAGCATCTCC
    GAGACCCAGA AACAGCTCTG TATCCAGCTG GAATTTCCCC ATCAGGAGAA CCCAGAAGCC
    GTGGCCCTCA GGCTGGCTCA CTGTGACCTG GGAACCCAAC AAAACCTCCT CGCCAGGAAG
    TTGATGGAGA CATGTGCCCG GCTGCGGCAG CTCAGTTTGT CCCAGGTGAA CCTCTGCGAT
    GCCAGCTCTC TGCTGCTGCA AAGCCTCCTG CTGTCCCTCT CTGAGCTGAA GAACTTCAGG
    CTGACCTCCA GCTGTGTGAG CTCCAAGGGG CTAGCCCTCC TGACATCTGG TCTGAGCCAT
    TGTCACCTTC TGGAGGAGCT AGACTTGTCT AACAATCAAC TTGGAGACGA GGGCACCAAG
    GTGTTGTTGG GGGCTCTTGA GGGCAAGTGC AGGCTGAAGA GACTTGACCT CAGCCACCTG
    CCACTGGGCG GCTCCACCCT GGCTGTGCTC ACTCAAGGAC TAGGCCACAT GACACTCCTG
    CAGAGCCTCC GTCTGAGCAA GAGCAACATC GATGACGTCG GCTGCTGCCA CCTTTCGAAG
    GCTCTCAGAG CTGCCACCAG CTTGGAGGAG CTGAGCTTGA GCCACAACCA GATCGGAAAC
    ATCGGTGCCC AGGACTTAGC TGCTGTCCTG CCGGGGCTCC CTGAGCTCAG GAAGATAGAC
    CTCTCAGCAA ATGGCATCGG CCCAGCAGGC GGAGCGCGGT TGGCAGAATC TCTCACCCTT
    TGCAGGCACC TCGAGGAGCT GATGCTTGGC TGCAATGCCC TGGGAGACTG CACAGCCCTG
    GGTCTGGCCA AGGGGCTGCC CCAGCACCTG AGGGTCCTGC ACCTGCCATC CAGCCATCTG
    AGCCCAGAGG GGGTGCTGAG TCTGGGCCAG GCCCTGGATG GATGCCCACA CGTGGAAGAG
    ATCAGCTTGG CAGAGAACAG CCTGGCTGGA GGGGTCCCGC ATTTCCATCA GGGGTTCCCA
    GTGCTCCGGA AGCTTGACCT GGTGTCGTGT GAGATTGACA ACCAGACCGC CAAGCCCCTC
    GCCGCCAGCT TCGTGCTCTG TCTGGCCCTG GAAGAAATCA CGCTGTCCTG GAATCTGCTC
    GGGGATGAGG CAGCTGCTGA GCTGGCCCGG GTCCTGCCGC AGATGGGCCG GCTGAAGAGA
    ATGGACCTCT GGAATAACCG TATTCCCCCG GACATGGCCC AGCATCTGCA GAACCAGGAG
    CCCAGGCTGG ACTTTGCTTT CTTTGACAAC CAGCCACAGG TCATCCCTCG GGGTACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57241
    Clone ID Related Accession (Same CDS sequence) XM_010823574.2 , XM_010814628.2
    Accession Version XM_010814628.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein NLRC5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814628.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG ACCTCTCAGG GAACCAGCTC AAAGATAAAG GCTGTCAACT GATGGCAGAG
    GCTGCTCCCC AACTGCACAT CACGAAGAAG CTGGACCTCA GCGACAATGA GCTTTCTGTG
    GCCGGGGTGC TGGGAGTACT GAGTGTGGTG AACACGTACC AAACCCTGAC AGAGCTACAT
    ATCAGCCTGC TGCACAAAAC TGTGGTCTTC ACGTTTGCCC CAGAGCTGGA GGAGCAGGAG
    GGGATCCAGA AGAGGGCTAC GTTTCCTGAC AGCCTTATGT TCCAGATGTC CTCTGAGCCA
    TCTCTGCGCT CCCCGAGGAT CAGGCTGACG CACTGTGCCC TGCAAGCCCA GCGCCTGGAG
    CTGCTCTGCA AAGCGCTGGG AGGAAGTTGC CGCCTCAGTC ACCTTGACTT ATCAGGCAAT
    GCTCTGGGGG ATGAAGGTGT GGCCCTGCTG GCTCGGCTGC TCCCCGGACT GGGTTCTCTG
    CAGTCCTTGA ACCTCAGTGA GAACCATTTG TCCCTGGAGG GTGTGTGCAG TTTGACCCAG
    TGCTTCTCTA CTCTGCGGTG GCTTCTACAT TTGGAATTCA GCTCTGCAAG CCAACACGTC
    ATCCTGAGAG GCGACAAAAG AGGCAGGGAT CTGTTGGCTG CTGGATCTTT GCCAGAGTTC
    CGAGCTGGAG CCCGGTTGGG ATCTGGTCAG CGCCACGTCC CCAGGAGCTT CTGCCTCAAG
    GAATGTCAGC TGGAGCCCCT GAGCCTCTCC TACCTCTGCA AGTCTCTGGA GAAGTGCCCA
    GGGCCCCTGG AAGTCCAATT GTCCTGCGAG GTGCTGAGTG ACCAGAGCCT GGAGACCCTG
    CTGTACCACC TACCGCAGCT CCCCCAGCTA AGCCTGCTGC AACTGAGCCA GATGCAACTG
    CCTCCAGAGA GCCCCCTCCT GCTAGCTGAC CTCTTCAGCC TGTGCCCACA GGTTCAGAAG
    GTGGATCTCA GGTCCCTGCG TCATTTGACC CTGCATCTCA GGTCTAGTGA GGAGCAGGAA
    GACAGGTGCT GTTGGTTCAC TGACTGCGGC CTCCGCCAGG AGCACGTGGA GCCGCTGTGC
    GGGTTGCTGA GCAAGTGTGA AGACCTCCAC CAGCTGGACC TCTCAGCAAA CCTGCTGGGT
    GATGATGGGC TCAGGTGCCT CCTGGAATGT CTCCCCAAGG TGCCCATTTC CGGTTCGCTT
    GATCTAAGCC ACAACAGTAT GTCTCAGGAA AGCGTCTTGT GCCTGGTGGA GGCGCTCCCC
    TCCTGCCCAC GTCTCCGGGA GGCCTCAGTG AACCTGGGCT CCAAGCAGAG CTTCTGGATT
    CACTTCTCCC GACAGGAAGA GACTGGGAAG ACACTGAGGC TGAGCGAGTG CCACTTCAAG
    CCAGAGCACA TGCCCAGACT GGCCGCCAGC CTGAGCCAGG CCCTGCAGCT GACGGAGCTC
    ACGCTGACCT GGTGTTTCCC GGGCCTGGAG CAGCTGACTG TCCTCCTGGG TCTGCTAACG
    TGGCCGACGG GGGTGCTCAC TCTCAGGGTG GAAGAGCCAT GGGTGGGCAG AGTCGGGGTG
    CTCACGCTGC TGGAAGTCTG TGCCCAGGCC TCAGGCAACA TCACTGGAAT AAGCATCTCC
    GAGACCCAGA AACAGCTCTG TATCCAGCTG GAATTTCCCC ATCAGGAGAA CCCAGAAGCC
    GTGGCCCTCA GGCTGGCTCA CTGTGACCTG GGAACCCAAC AAAACCTCCT CGCCAGGAAG
    TTGATGGAGA CATGTGCCCG GCTGCGGCAG CTCAGTTTGT CCCAGGTGAA CCTCTGCGAT
    GCCAGCTCTC TGCTGCTGCA AAGCCTCCTG CTGTCCCTCT CTGAGCTGAA GAACTTCAGG
    CTGACCTCCA GCTGTGTGAG CTCCAAGGGG CTAGCCCTCC TGACATCTGG TCTGAGCCAT
    TGTCACCTTC TGGAGGAGCT AGACTTGTCT AACAATCAAC TTGGAGACGA GGGCACCAAG
    GTGTTGTTGG GGGCTCTTGA GGGCAAGTGC AGGCTGAAGA GACTTGACCT CAGCCACCTG
    CCACTGGGCG GCTCCACCCT GGCTGTGCTC ACTCAAGGAC TAGGCCACAT GACACTCCTG
    CAGAGCCTCC GTCTGAGCAA GAGCAACATC GATGACGTCG GCTGCTGCCA CCTTTCGAAG
    GCTCTCAGAG CTGCCACCAG CTTGGAGGAG CTGAGCTTGA GCCACAACCA GATCGGAAAC
    ATCGGTGCCC AGGACTTAGC TGCTGTCCTG CCGGGGCTCC CTGAGCTCAG GAAGATAGAC
    CTCTCAGCAA ATGGCATCGG CCCAGCAGGC GGAGCGCGGT TGGCAGAATC TCTCACCCTT
    TGCAGGCACC TCGAGGAGCT GATGCTTGGC TGCAATGCCC TGGGAGACTG CACAGCCCTG
    GGTCTGGCCA AGGGGCTGCC CCAGCACCTG AGGGTCCTGC ACCTGCCATC CAGCCATCTG
    AGCCCAGAGG GGGTGCTGAG TCTGGGCCAG GCCCTGGATG GATGCCCACA CGTGGAAGAG
    ATCAGCTTGG CAGAGAACAG CCTGGCTGGA GGGGTCCCGC ATTTCCATCA GGGGTTCCCA
    GTGCTCCGGA AGCTTGACCT GGTGTCGTGT GAGATTGACA ACCAGACCGC CAAGCCCCTC
    GCCGCCAGCT TCGTGCTCTG TCTGGCCCTG GAAGAAATCA CGCTGTCCTG GAATCTGCTC
    GGGGATGAGG CAGCTGCTGA GCTGGCCCGG GTCCTGCCGC AGATGGGCCG GCTGAAGAGA
    ATGGACCTCT GGAATAACCG TATTCCCCCG GACATGGCCC AGCATCTGCA GAACCAGGAG
    CCCAGGCTGG ACTTTGCTTT CTTTGACAAC CAGCCACAGG TCATCCCTCG GGGTACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812930.1
    CDS552..6071
    Translation

    Target ORF information:

    RefSeq Version XM_010814628.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NLR family, CARD domain containing 5 (NLRC5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814628.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGACCCCG TTAGCCTCCA ACTGGGCACC AAGAACCTGC ATGGATATCT CGTGAGGCTG 
    CTCAGCAGAA ACCTAGGATG GCTAGACGCC AAGGTGAAGT TATTCCTCCC CAGCGTGGAC
    CTGGGCTCCG GATATCAGGC CCTAGACCCC ACACAGAGGG TCATCCTGCA ACTCCAAAAA
    CTGCATGCCC AGGGTCCGAC CACCTGGAAG TCATTCATCC AGTGCGTGTG CATGGAGCTG
    GATGTGCCAA TGGACATGGA GGTGCTGCTG ATGAGCACGT GGGGCCACGA AGATGGCTTC
    TCCAGTCTTC TGGAAATTGA CGAGGAAAGC CAATCAGACT CTCAGCTCCA CCCTGGCCTC
    AAGCGGACTC ATCAGAGCTG TGGGTCCTCA CCTCGCCCAA AACAGTTCAG GAAGCAGCAA
    CAAGAGTTGG CCAAGAGGTA CCTGCAGCTA CTGAGAACTT CTGCCCAGCA GCGCTATGGC
    AACAGGACCC CCGGGCCGGG ACAGCCATTG GCCTTCCACC AAGCCTACGT CCCTCCAATC
    TTGAAATGGA GCCGGGCCTC GGCGCCCTTC CACACTCAGG AGGGTGCCAC AACAGGGAAC
    CCCAAGGCAG AAGACGGCAC TGATGTGAAC GTTCGGGACC TCTTCAGTAC CAAGGCCAGC
    AAGGGCCCGA GAGTGACGGT GCTTCTGGGG AAGGCGGGCA TGGGCAAGAC CACGCTGGCT
    CACCGGCTCT GCCAGAAGTG GGCCGATGGT CAGCTGGACC GCTTCCAGGC TGTGTTCCTT
    TTCGAATTCC GCCAGCTCAA CCTGATCACA GGTTCCTTGA CACTGCCGCA GCTCCTTTTC
    GACATGTACC TAAGGCCTGA GGCCAGCCCG GAGGCCGTCT TCCAGCACCT GAAGGAGAAC
    GCGGAGGGAA TCCTGCTGAT CTTCGACGGG CTGGACGAGA TCCTCCAGCC CTGCTCTGAC
    AAGGAGACCG AGGACCCCGA GGACCCAGCG TCAGCCCTCA CCCTCTTCTC CCGCCTCTGC
    CGTGGGACCC TGCTGCCCGG CTGCTCGGTG ATGGCCACCT CCCGTCCAGG GAAGCTGCCC
    GCCTGCCTGC CCACCGAGGC GGCCACGGTC CACATGTGGG GCTTCGACGG CCCGCGGGTG
    GAGGAGTATG TGAACCACTT CTTCAGCGAC CAGCCGTCCC AGGAGGCGGC CCTGGCGGAG
    CTGCAGATGG ACAGACACCT CTGGAGCATG TGTGCAGTGC CTGCGCTGTG CCGAGTCGCC
    TGCCTCTGCC TCCATCACCT GCTCCCGGGC AGGTCTCGAG GCCAGTCTGC AGCCCTCCTG
    CCCACCATGA CGCAGAACTA CATGCAGATG CTCCTCACCC TCAACCCCCA AGGGGTCCTG
    CGGGCTGAGC CCCTGCTGGG TCTCGGGGAG GTGGCCCTGA GCGGCCTGGA GTCAGGGAAG
    GTCATCTTCT CTGCAGGAGA CATCCCTGCA TCCACCCTAG ACTTTGGGGC TGCCCTCGGT
    CTGCTGACCT CCTTCTGCGT GTGCCTGGGG CCCGGGCACC GGGACACAGG CTACGCCTTC
    TCCCACCTCA GCCTGCAGGA GTTCTTCGCA GCCCTGCACC TGCTGGCCAG CCCCCAGGTG
    GACAGAGAGG CCCTCAGCCA ACATGTCACC CTCCATTCTC GCTGGGTGCA GCGGACAAAA
    GCCAGACTGG GCCTGTCGGA CCACCTGCCC ACCTTTCTGG CTGGCCTGGC ATCCCGTGCC
    TGCCGCTCCT TCCTCAGCCT CCTGGCTGAG CGGGAGGAGG AATGGGTGGC TGCCAAGCAG
    GAGGTAGTCC TGCAGGCGCT GAAGAAGTTG GCCACCCGTC GGCTCACGGG GCCCAAGGTT
    GTAGAGCTGT GTCACCGTGT GGGTGAGACG CAGGATCCTG AGTTGGCCAG CTTCATGGCC
    CGCAGCCTCC CCCAGCAGCT GCCCTTCCAC AACTTCCTGC TGACCTACGC CGACCTGGCC
    TCCCTGACCA GCATCCTGGG GCACAGGGAT GGCCCCATCC ACCTGGATTT TGAGGGCTGC
    CCCCTGGAGC CCCGCTGCCC TGAAACCCTG GCAGGCTGTG GGCAGGTAGA GAATCTCAGC
    TTTAAGAGTC GGAAGTGTGG GGACGCCTTT GCAGAAGCCC TCTCCAAGAG CTTGCCAACG
    ATGGGGAGCC TGAAGAAGCT GGGGTTAGCA GGAAGTAAGA TCACTGCTCA AGGCATCAGC
    CACCTGGTGC AGGCTTTGCC CTTCTGCCCA CAGCTGGAGG AGGTCAGCTT CCAGGACAAT
    CAGCTCAAGG ACAGGGAAGT GCTGAACATC GTGGAGGTAC TCTCTTGCCT ACCACGGCTC
    CAGAAACTTG ACCTGAGCCG CAACAATGTC TCCGTGTCAA CACTACTCTC CTTGACAAAG
    GTGGCGGTCA CGTGCCCTAC CATCAGGATG CTGCAGGTCA GGGAGACGGA CCTCATCTTC
    CTCCTCTCCC CGCCCACAGA GACAGATCCT GAGCTACAAG GAGACCCAGA CTTACAGGGA
    AATGCCAACC AGAGGAAAGA GGCTCAGAGA AGAAGCCTGG CACTCAGGCT CCAGAAGTGT
    CAGCTTGGGG TCCATGATGT GGAGGTGCTC ATAGCCCAGC TGCGGGAAGG CCCTCAACTG
    GATGAAGTGG ACCTCTCAGG GAACCAGCTC AAAGATAAAG GCTGTCAACT GATGGCAGAG
    GCTGCTCCCC AACTGCACAT CACGAAGAAG CTGGACCTCA GCGACAATGA GCTTTCTGTG
    GCCGGGGTGC TGGGAGTACT GAGTGTGGTG AACACGTACC AAACCCTGAC AGAGCTACAT
    ATCAGCCTGC TGCACAAAAC TGTGGTCTTC ACGTTTGCCC CAGAGCTGGA GGAGCAGGAG
    GGGATCCAGA AGAGGGCTAC GTTTCCTGAC AGCCTTATGT TCCAGATGTC CTCTGAGCCA
    TCTCTGCGCT CCCCGAGGAT CAGGCTGACG CACTGTGCCC TGCAAGCCCA GCGCCTGGAG
    CTGCTCTGCA AAGCGCTGGG AGGAAGTTGC CGCCTCAGTC ACCTTGACTT ATCAGGCAAT
    GCTCTGGGGG ATGAAGGTGT GGCCCTGCTG GCTCGGCTGC TCCCCGGACT GGGTTCTCTG
    CAGTCCTTGA ACCTCAGTGA GAACCATTTG TCCCTGGAGG GTGTGTGCAG TTTGACCCAG
    TGCTTCTCTA CTCTGCGGTG GCTTCTACAT TTGGAATTCA GCTCTGCAAG CCAACACGTC
    ATCCTGAGAG GCGACAAAAG AGGCAGGGAT CTGTTGGCTG CTGGATCTTT GCCAGAGTTC
    CGAGCTGGAG CCCGGTTGGG ATCTGGTCAG CGCCACGTCC CCAGGAGCTT CTGCCTCAAG
    GAATGTCAGC TGGAGCCCCT GAGCCTCTCC TACCTCTGCA AGTCTCTGGA GAAGTGCCCA
    GGGCCCCTGG AAGTCCAATT GTCCTGCGAG GTGCTGAGTG ACCAGAGCCT GGAGACCCTG
    CTGTACCACC TACCGCAGCT CCCCCAGCTA AGCCTGCTGC AACTGAGCCA GATGCAACTG
    CCTCCAGAGA GCCCCCTCCT GCTAGCTGAC CTCTTCAGCC TGTGCCCACA GGTTCAGAAG
    GTGGATCTCA GGTCCCTGCG TCATTTGACC CTGCATCTCA GGTCTAGTGA GGAGCAGGAA
    GACAGGTGCT GTTGGTTCAC TGACTGCGGC CTCCGCCAGG AGCACGTGGA GCCGCTGTGC
    GGGTTGCTGA GCAAGTGTGA AGACCTCCAC CAGCTGGACC TCTCAGCAAA CCTGCTGGGT
    GATGATGGGC TCAGGTGCCT CCTGGAATGT CTCCCCAAGG TGCCCATTTC CGGTTCGCTT
    GATCTAAGCC ACAACAGTAT GTCTCAGGAA AGCGTCTTGT GCCTGGTGGA GGCGCTCCCC
    TCCTGCCCAC GTCTCCGGGA GGCCTCAGTG AACCTGGGCT CCAAGCAGAG CTTCTGGATT
    CACTTCTCCC GACAGGAAGA GACTGGGAAG ACACTGAGGC TGAGCGAGTG CCACTTCAAG
    CCAGAGCACA TGCCCAGACT GGCCGCCAGC CTGAGCCAGG CCCTGCAGCT GACGGAGCTC
    ACGCTGACCT GGTGTTTCCC GGGCCTGGAG CAGCTGACTG TCCTCCTGGG TCTGCTAACG
    TGGCCGACGG GGGTGCTCAC TCTCAGGGTG GAAGAGCCAT GGGTGGGCAG AGTCGGGGTG
    CTCACGCTGC TGGAAGTCTG TGCCCAGGCC TCAGGCAACA TCACTGGAAT AAGCATCTCC
    GAGACCCAGA AACAGCTCTG TATCCAGCTG GAATTTCCCC ATCAGGAGAA CCCAGAAGCC
    GTGGCCCTCA GGCTGGCTCA CTGTGACCTG GGAACCCAAC AAAACCTCCT CGCCAGGAAG
    TTGATGGAGA CATGTGCCCG GCTGCGGCAG CTCAGTTTGT CCCAGGTGAA CCTCTGCGAT
    GCCAGCTCTC TGCTGCTGCA AAGCCTCCTG CTGTCCCTCT CTGAGCTGAA GAACTTCAGG
    CTGACCTCCA GCTGTGTGAG CTCCAAGGGG CTAGCCCTCC TGACATCTGG TCTGAGCCAT
    TGTCACCTTC TGGAGGAGCT AGACTTGTCT AACAATCAAC TTGGAGACGA GGGCACCAAG
    GTGTTGTTGG GGGCTCTTGA GGGCAAGTGC AGGCTGAAGA GACTTGACCT CAGCCACCTG
    CCACTGGGCG GCTCCACCCT GGCTGTGCTC ACTCAAGGAC TAGGCCACAT GACACTCCTG
    CAGAGCCTCC GTCTGAGCAA GAGCAACATC GATGACGTCG GCTGCTGCCA CCTTTCGAAG
    GCTCTCAGAG CTGCCACCAG CTTGGAGGAG CTGAGCTTGA GCCACAACCA GATCGGAAAC
    ATCGGTGCCC AGGACTTAGC TGCTGTCCTG CCGGGGCTCC CTGAGCTCAG GAAGATAGAC
    CTCTCAGCAA ATGGCATCGG CCCAGCAGGC GGAGCGCGGT TGGCAGAATC TCTCACCCTT
    TGCAGGCACC TCGAGGAGCT GATGCTTGGC TGCAATGCCC TGGGAGACTG CACAGCCCTG
    GGTCTGGCCA AGGGGCTGCC CCAGCACCTG AGGGTCCTGC ACCTGCCATC CAGCCATCTG
    AGCCCAGAGG GGGTGCTGAG TCTGGGCCAG GCCCTGGATG GATGCCCACA CGTGGAAGAG
    ATCAGCTTGG CAGAGAACAG CCTGGCTGGA GGGGTCCCGC ATTTCCATCA GGGGTTCCCA
    GTGCTCCGGA AGCTTGACCT GGTGTCGTGT GAGATTGACA ACCAGACCGC CAAGCCCCTC
    GCCGCCAGCT TCGTGCTCTG TCTGGCCCTG GAAGAAATCA CGCTGTCCTG GAATCTGCTC
    GGGGATGAGG CAGCTGCTGA GCTGGCCCGG GTCCTGCCGC AGATGGGCCG GCTGAAGAGA
    ATGGACCTCT GGAATAACCG TATTCCCCCG GACATGGCCC AGCATCTGCA GAACCAGGAG
    CCCAGGCTGG ACTTTGCTTT CTTTGACAAC CAGCCACAGG TCATCCCTCG GGGTACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.