AASDH cDNA ORF clone, Bos taurus(cattle)

The following AASDH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AASDH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa57190 XM_010797927.2
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa57190 XM_010806148.2
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa235566 XM_024993577.1
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa235567 XM_024993578.1
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa235568 XM_024993579.1
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa235569 XM_024993580.1
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa235570 XM_010806148.3
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa90092 XM_015463766.1
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa90092 XM_015471629.1
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa235563 XM_002688264.6
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa235564 XM_024993575.1
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa235565 XM_024993576.1
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa90089 XM_015471626.1
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa90089 XM_015463763.1
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa90090 XM_015471627.1
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa90090 XM_015463764.1
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa90091 XM_015471628.1
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa90091 XM_015463765.1
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa04809 XM_001250588.5
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa04809 XM_002688264.5
Latest version!
Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa57190
    Clone ID Related Accession (Same CDS sequence) XM_010806148.2 , XM_010797927.2
    Accession Version XM_010806148.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2052bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product acyl-CoA synthetase family member 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103975.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806148.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAGAGCCA CAGGAGACTT TGTGACTATA AAAGATGGAG AGATATTTTT CTTGGGACGA 
    AAGGACAGTC AGATCAAACG TCATGGCAAA CGTCTTAACA TAGAACTTGT ACAACAGATT
    GCTGAAGGTC TTCAACAAGT GGAGTCTTGT GCAGTTACAT GGTATAACCA GGAAAAATTG
    ATTTTGTTCA TGGTGACTAA AAATGATTTA GTAAAGGATC ACATCTTTGA AGAACTGCAG
    AAACATCTTC CAAGTCATGC CATCCCAGAT GAGCTTGTGT TAATTAATTC TCTGCCATTT
    ACATCTCATG GCAAAATTGA TGTTTCTGAG TTAAATAAGA TATATTTAAA CTACAGAGAC
    TTGAAGTCTG AGTGTAAGCT CAGTGGAAAA GAGGAACTTT GGGAAAAATT ACACCATTTG
    TGGAAGTCTG TTCTGAGTCT CTCAGAAGAT GCTTTGAAGA TTCCTGATGA GTCACTCTTC
    TTAAATAGTG GTGGAGATTC CTTGAAATCC ATACGGCTTC TCAATGAGAT TGAAAACCTT
    GTTGGCACAT CAGTACCTGG GCTTCTGGAA ATTATTCTCA GCAGTTCTAT TTTACGGATT
    TACAATCACA TCCTTCAAAC AGTGTTTTCA GATAAAGATA TGACACTTAG CAAGAATCAT
    GCCATGAAAA GAAAATTCAA CGTTACTCAA GAGGAAACCA GTGGAAAATC TTTACATCAG
    GAAACTGTCA TGCCTTTAAA TTGTGACAAG GAGATCGATG CTTTTATTGC ACTGAGCAGA
    GGGAATCAGA TTTTGTCTTT GAATACTGAT AAGTATTTAA CTAAGTTGGG ACATTGCCCT
    TCAACCTATT CTTCTGATTT AAATTCACAG ACTGACATTC AAAATAGAGA AAGCCTAAAT
    ACTCCACCTC TTATTGGGAA GTCAGAAGAT CCATCCTGTG TTGTGGAAGT TTCTGAAGAG
    GAAACACCTG TGATTAGGGC TGAGAAAATG GAGTTTCGTG TGAGGTGGAG GTCAGACACA
    GGCAAATGTG TGGATGCTTC ACCTCTGGTT GTAATACCAG CTATTGATAA GTCATCTGCA
    ACTGTGTACA TTGGCTCTCA TTCTCATAGA ATGATAGCAG TTGATCTTTA CTCTGGAAAG
    GTGAAATGGG AACAGATTTT AGGAGATCGC ATTGAATCCT CAGCATGTGT ATCTAAGTGT
    GGAAACTTTA TTGTAGTGGG CTGTTATAAT GGGTTAGTTT ATGTTCTGAA AAGTAATAGT
    GGAGAAAAAT ACTGGATGTT TAGTACTGAG GATGCTGTCA AAAGCTCAGC AACCATGGAT
    CCAACCACAG GACTTCTTTA CATTGGATCT CATGACCAGC ATGCATACGC TTTGGATATA
    TATAAGAAGA AGTGTGTTTG GAAATTGAAA TGTGGAGGAA CTGTTTTTTC TTCTCCTTGT
    TTGAGCCTGA TTCCATCTCA CTTGTATTTT GCTACACTGG GAGGACTTTT ACTGGCTATA
    AATCCTGCCA CTGGGGACAG ACTTTGGAAA CATTCCTGTG GAAAGCCACT CTTTTCTTCT
    CCACGGTGTT GCCTACAGTA TGTTTTTATT GGCTGTGTAG ATGGAAATTT ACTCTGCTTT
    ACTCACTTTG GAGAACAGGT TTGGCAATTC TCTACCAATG AACCAATCTT TTCATCCCCA
    TGTACCTCAG CATCAGAGCA AGAAATATTT TTTGGTTCTC ATGACTGCTT TATCTACTGT
    TGTAGTATGA AAGGTCACCT CCAGTGGAAA TTTGAAACTA CTTCAAGAGT GTATTCAACG
    CCTTTTGTTT TCCGTAACCA CAACCATAAC AGTGAAATGT TACTGGCAGC GGCATCTACT
    GATGGGAAAC TGTGGATCTT GGAATCTAAG AGTGGACAGT TGCAAAGTGT GTATGAACTT
    CCTGGAGAAG TTTTCTCTTC TCCTGTGGTC TGGGAATCAA TGCTTATTAT TGGATGTAGA
    AATAATTATG TTTATTGTCT GGATGTATTG GGTGACAAAG GTAATGAAGT GCAGTCCTTA
    GAATATACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804450.1
    CDS163..2214
    Translation

    Target ORF information:

    RefSeq Version XM_010806148.2
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806148.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAGAGCCA CAGGAGACTT TGTGACTATA AAAGATGGAG AGATATTTTT CTTGGGACGA 
    AAGGACAGTC AGATCAAACG TCATGGCAAA CGTCTTAACA TAGAACTTGT ACAACAGATT
    GCTGAAGGTC TTCAACAAGT GGAGTCTTGT GCAGTTACAT GGTATAACCA GGAAAAATTG
    ATTTTGTTCA TGGTGACTAA AAATGATTTA GTAAAGGATC ACATCTTTGA AGAACTGCAG
    AAACATCTTC CAAGTCATGC CATCCCAGAT GAGCTTGTGT TAATTAATTC TCTGCCATTT
    ACATCTCATG GCAAAATTGA TGTTTCTGAG TTAAATAAGA TATATTTAAA CTACAGAGAC
    TTGAAGTCTG AGTGTAAGCT CAGTGGAAAA GAGGAACTTT GGGAAAAATT ACACCATTTG
    TGGAAGTCTG TTCTGAGTCT CTCAGAAGAT GCTTTGAAGA TTCCTGATGA GTCACTCTTC
    TTAAATAGTG GTGGAGATTC CTTGAAATCC ATACGGCTTC TCAATGAGAT TGAAAACCTT
    GTTGGCACAT CAGTACCTGG GCTTCTGGAA ATTATTCTCA GCAGTTCTAT TTTACGGATT
    TACAATCACA TCCTTCAAAC AGTGTTTTCA GATAAAGATA TGACACTTAG CAAGAATCAT
    GCCATGAAAA GAAAATTCAA CGTTACTCAA GAGGAAACCA GTGGAAAATC TTTACATCAG
    GAAACTGTCA TGCCTTTAAA TTGTGACAAG GAGATCGATG CTTTTATTGC ACTGAGCAGA
    GGGAATCAGA TTTTGTCTTT GAATACTGAT AAGTATTTAA CTAAGTTGGG ACATTGCCCT
    TCAACCTATT CTTCTGATTT AAATTCACAG ACTGACATTC AAAATAGAGA AAGCCTAAAT
    ACTCCACCTC TTATTGGGAA GTCAGAAGAT CCATCCTGTG TTGTGGAAGT TTCTGAAGAG
    GAAACACCTG TGATTAGGGC TGAGAAAATG GAGTTTCGTG TGAGGTGGAG GTCAGACACA
    GGCAAATGTG TGGATGCTTC ACCTCTGGTT GTAATACCAG CTATTGATAA GTCATCTGCA
    ACTGTGTACA TTGGCTCTCA TTCTCATAGA ATGATAGCAG TTGATCTTTA CTCTGGAAAG
    GTGAAATGGG AACAGATTTT AGGAGATCGC ATTGAATCCT CAGCATGTGT ATCTAAGTGT
    GGAAACTTTA TTGTAGTGGG CTGTTATAAT GGGTTAGTTT ATGTTCTGAA AAGTAATAGT
    GGAGAAAAAT ACTGGATGTT TAGTACTGAG GATGCTGTCA AAAGCTCAGC AACCATGGAT
    CCAACCACAG GACTTCTTTA CATTGGATCT CATGACCAGC ATGCATACGC TTTGGATATA
    TATAAGAAGA AGTGTGTTTG GAAATTGAAA TGTGGAGGAA CTGTTTTTTC TTCTCCTTGT
    TTGAGCCTGA TTCCATCTCA CTTGTATTTT GCTACACTGG GAGGACTTTT ACTGGCTATA
    AATCCTGCCA CTGGGGACAG ACTTTGGAAA CATTCCTGTG GAAAGCCACT CTTTTCTTCT
    CCACGGTGTT GCCTACAGTA TGTTTTTATT GGCTGTGTAG ATGGAAATTT ACTCTGCTTT
    ACTCACTTTG GAGAACAGGT TTGGCAATTC TCTACCAATG AACCAATCTT TTCATCCCCA
    TGTACCTCAG CATCAGAGCA AGAAATATTT TTTGGTTCTC ATGACTGCTT TATCTACTGT
    TGTAGTATGA AAGGTCACCT CCAGTGGAAA TTTGAAACTA CTTCAAGAGT GTATTCAACG
    CCTTTTGTTT TCCGTAACCA CAACCATAAC AGTGAAATGT TACTGGCAGC GGCATCTACT
    GATGGGAAAC TGTGGATCTT GGAATCTAAG AGTGGACAGT TGCAAAGTGT GTATGAACTT
    CCTGGAGAAG TTTTCTCTTC TCCTGTGGTC TGGGAATCAA TGCTTATTAT TGGATGTAGA
    AATAATTATG TTTATTGTCT GGATGTATTG GGTGACAAAG GTAATGAAGT GCAGTCCTTA
    GAATATACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa57190
    Clone ID Related Accession (Same CDS sequence) XM_010806148.2 , XM_010797927.2
    Accession Version XM_010797927.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2052bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product acyl-CoA synthetase family member 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010797927.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAGAGCCA CAGGAGACTT TGTGACTATA AAAGATGGAG AGATATTTTT CTTGGGACGA 
    AAGGACAGTC AGATCAAACG TCATGGCAAA CGTCTTAACA TAGAACTTGT ACAACAGATT
    GCTGAAGGTC TTCAACAAGT GGAGTCTTGT GCAGTTACAT GGTATAACCA GGAAAAATTG
    ATTTTGTTCA TGGTGACTAA AAATGATTTA GTAAAGGATC ACATCTTTGA AGAACTGCAG
    AAACATCTTC CAAGTCATGC CATCCCAGAT GAGCTTGTGT TAATTAATTC TCTGCCATTT
    ACATCTCATG GCAAAATTGA TGTTTCTGAG TTAAATAAGA TATATTTAAA CTACAGAGAC
    TTGAAGTCTG AGTGTAAGCT CAGTGGAAAA GAGGAACTTT GGGAAAAATT ACACCATTTG
    TGGAAGTCTG TTCTGAGTCT CTCAGAAGAT GCTTTGAAGA TTCCTGATGA GTCACTCTTC
    TTAAATAGTG GTGGAGATTC CTTGAAATCC ATACGGCTTC TCAATGAGAT TGAAAACCTT
    GTTGGCACAT CAGTACCTGG GCTTCTGGAA ATTATTCTCA GCAGTTCTAT TTTACGGATT
    TACAATCACA TCCTTCAAAC AGTGTTTTCA GATAAAGATA TGACACTTAG CAAGAATCAT
    GCCATGAAAA GAAAATTCAA CGTTACTCAA GAGGAAACCA GTGGAAAATC TTTACATCAG
    GAAACTGTCA TGCCTTTAAA TTGTGACAAG GAGATCGATG CTTTTATTGC ACTGAGCAGA
    GGGAATCAGA TTTTGTCTTT GAATACTGAT AAGTATTTAA CTAAGTTGGG ACATTGCCCT
    TCAACCTATT CTTCTGATTT AAATTCACAG ACTGACATTC AAAATAGAGA AAGCCTAAAT
    ACTCCACCTC TTATTGGGAA GTCAGAAGAT CCATCCTGTG TTGTGGAAGT TTCTGAAGAG
    GAAACACCTG TGATTAGGGC TGAGAAAATG GAGTTTCGTG TGAGGTGGAG GTCAGACACA
    GGCAAATGTG TGGATGCTTC ACCTCTGGTT GTAATACCAG CTATTGATAA GTCATCTGCA
    ACTGTGTACA TTGGCTCTCA TTCTCATAGA ATGATAGCAG TTGATCTTTA CTCTGGAAAG
    GTGAAATGGG AACAGATTTT AGGAGATCGC ATTGAATCCT CAGCATGTGT ATCTAAGTGT
    GGAAACTTTA TTGTAGTGGG CTGTTATAAT GGGTTAGTTT ATGTTCTGAA AAGTAATAGT
    GGAGAAAAAT ACTGGATGTT TAGTACTGAG GATGCTGTCA AAAGCTCAGC AACCATGGAT
    CCAACCACAG GACTTCTTTA CATTGGATCT CATGACCAGC ATGCATACGC TTTGGATATA
    TATAAGAAGA AGTGTGTTTG GAAATTGAAA TGTGGAGGAA CTGTTTTTTC TTCTCCTTGT
    TTGAGCCTGA TTCCATCTCA CTTGTATTTT GCTACACTGG GAGGACTTTT ACTGGCTATA
    AATCCTGCCA CTGGGGACAG ACTTTGGAAA CATTCCTGTG GAAAGCCACT CTTTTCTTCT
    CCACGGTGTT GCCTACAGTA TGTTTTTATT GGCTGTGTAG ATGGAAATTT ACTCTGCTTT
    ACTCACTTTG GAGAACAGGT TTGGCAATTC TCTACCAATG AACCAATCTT TTCATCCCCA
    TGTACCTCAG CATCAGAGCA AGAAATATTT TTTGGTTCTC ATGACTGCTT TATCTACTGT
    TGTAGTATGA AAGGTCACCT CCAGTGGAAA TTTGAAACTA CTTCAAGAGT GTATTCAACG
    CCTTTTGTTT TCCGTAACCA CAACCATAAC AGTGAAATGT TACTGGCAGC GGCATCTACT
    GATGGGAAAC TGTGGATCTT GGAATCTAAG AGTGGACAGT TGCAAAGTGT GTATGAACTT
    CCTGGAGAAG TTTTCTCTTC TCCTGTGGTC TGGGAATCAA TGCTTATTAT TGGATGTAGA
    AATAATTATG TTTATTGTCT GGATGTATTG GGTGACAAAG GTAATGAAGT GCAGTCCTTA
    GAATATACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796229.1
    CDS163..2214
    Translation

    Target ORF information:

    RefSeq Version XM_010797927.2
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010797927.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAGAGCCA CAGGAGACTT TGTGACTATA AAAGATGGAG AGATATTTTT CTTGGGACGA 
    AAGGACAGTC AGATCAAACG TCATGGCAAA CGTCTTAACA TAGAACTTGT ACAACAGATT
    GCTGAAGGTC TTCAACAAGT GGAGTCTTGT GCAGTTACAT GGTATAACCA GGAAAAATTG
    ATTTTGTTCA TGGTGACTAA AAATGATTTA GTAAAGGATC ACATCTTTGA AGAACTGCAG
    AAACATCTTC CAAGTCATGC CATCCCAGAT GAGCTTGTGT TAATTAATTC TCTGCCATTT
    ACATCTCATG GCAAAATTGA TGTTTCTGAG TTAAATAAGA TATATTTAAA CTACAGAGAC
    TTGAAGTCTG AGTGTAAGCT CAGTGGAAAA GAGGAACTTT GGGAAAAATT ACACCATTTG
    TGGAAGTCTG TTCTGAGTCT CTCAGAAGAT GCTTTGAAGA TTCCTGATGA GTCACTCTTC
    TTAAATAGTG GTGGAGATTC CTTGAAATCC ATACGGCTTC TCAATGAGAT TGAAAACCTT
    GTTGGCACAT CAGTACCTGG GCTTCTGGAA ATTATTCTCA GCAGTTCTAT TTTACGGATT
    TACAATCACA TCCTTCAAAC AGTGTTTTCA GATAAAGATA TGACACTTAG CAAGAATCAT
    GCCATGAAAA GAAAATTCAA CGTTACTCAA GAGGAAACCA GTGGAAAATC TTTACATCAG
    GAAACTGTCA TGCCTTTAAA TTGTGACAAG GAGATCGATG CTTTTATTGC ACTGAGCAGA
    GGGAATCAGA TTTTGTCTTT GAATACTGAT AAGTATTTAA CTAAGTTGGG ACATTGCCCT
    TCAACCTATT CTTCTGATTT AAATTCACAG ACTGACATTC AAAATAGAGA AAGCCTAAAT
    ACTCCACCTC TTATTGGGAA GTCAGAAGAT CCATCCTGTG TTGTGGAAGT TTCTGAAGAG
    GAAACACCTG TGATTAGGGC TGAGAAAATG GAGTTTCGTG TGAGGTGGAG GTCAGACACA
    GGCAAATGTG TGGATGCTTC ACCTCTGGTT GTAATACCAG CTATTGATAA GTCATCTGCA
    ACTGTGTACA TTGGCTCTCA TTCTCATAGA ATGATAGCAG TTGATCTTTA CTCTGGAAAG
    GTGAAATGGG AACAGATTTT AGGAGATCGC ATTGAATCCT CAGCATGTGT ATCTAAGTGT
    GGAAACTTTA TTGTAGTGGG CTGTTATAAT GGGTTAGTTT ATGTTCTGAA AAGTAATAGT
    GGAGAAAAAT ACTGGATGTT TAGTACTGAG GATGCTGTCA AAAGCTCAGC AACCATGGAT
    CCAACCACAG GACTTCTTTA CATTGGATCT CATGACCAGC ATGCATACGC TTTGGATATA
    TATAAGAAGA AGTGTGTTTG GAAATTGAAA TGTGGAGGAA CTGTTTTTTC TTCTCCTTGT
    TTGAGCCTGA TTCCATCTCA CTTGTATTTT GCTACACTGG GAGGACTTTT ACTGGCTATA
    AATCCTGCCA CTGGGGACAG ACTTTGGAAA CATTCCTGTG GAAAGCCACT CTTTTCTTCT
    CCACGGTGTT GCCTACAGTA TGTTTTTATT GGCTGTGTAG ATGGAAATTT ACTCTGCTTT
    ACTCACTTTG GAGAACAGGT TTGGCAATTC TCTACCAATG AACCAATCTT TTCATCCCCA
    TGTACCTCAG CATCAGAGCA AGAAATATTT TTTGGTTCTC ATGACTGCTT TATCTACTGT
    TGTAGTATGA AAGGTCACCT CCAGTGGAAA TTTGAAACTA CTTCAAGAGT GTATTCAACG
    CCTTTTGTTT TCCGTAACCA CAACCATAAC AGTGAAATGT TACTGGCAGC GGCATCTACT
    GATGGGAAAC TGTGGATCTT GGAATCTAAG AGTGGACAGT TGCAAAGTGT GTATGAACTT
    CCTGGAGAAG TTTTCTCTTC TCCTGTGGTC TGGGAATCAA TGCTTATTAT TGGATGTAGA
    AATAATTATG TTTATTGTCT GGATGTATTG GGTGACAAAG GTAATGAAGT GCAGTCCTTA
    GAATATACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235566
    Clone ID Related Accession (Same CDS sequence) XM_024993577.1
    Accession Version XM_024993577.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product beta-alanine-activating enzyme isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTA AATTCAAGTC TTCCTATGAA GCATTATTGA ACTATGATAT GCTTAAAGTA
    GAACATAATG ACCTAGTACT CTTCAGACTT CACTGGAAAA ATGTCGAGGT GAGCCTGGTG
    CTAAATGATA GAAAAGAGAA ATATGAAAAA GGAAAGATGC CAAAGAGTAT GCATTCTGAG
    AGCAGCAGTG AAGAAAAGTC AGAAGAGCAC ATGGATATGA GGCAAAAGCA CTGCTTGGCC
    TATGTTCTCC ATACGTCAGG CACTACAGGG ATACCTAAGA TTGTCAGAGT CCCTCATGCA
    TGTATAGTGC CAAACATCCA GCATTTTCGA TGTGAATTGC CTGTACAACT GGGATTCCCA
    CTGCTTGGAA CAGTAGTTGA AGTCAGAGAT ACTAACGGTT TCACAATTCA AGAAGGCAAT
    GGCCAAGTAT TTTTAGGTGG CAGAAATAGA GTATGTTTTC TCGATGGCGA AGTGACGGTA
    CCACTTGGCA CAATGAGAGC CACAGGAGAC TTTGTGACTA TAAAAGATGG AGAGATATTT
    TTCTTGGGAC GAAAGGACAG TCAGATCAAA CGTCATGGCA AACGTCTTAA CATAGAACTT
    GTACAACAGA TTGCTGAAGG TCTTCAACAA GTGGAGTCTT GTGCAGTTAC ATGGTATAAC
    CAGGAAAAAT TGATTTTGTT CATGGTGACT AAAAATGATT TAGTAAAGGA TCACATCTTT
    GAAGAACTGC AGAAACATCT TCCAAGTCAT GCCATCCCAG ATGAGCTTGT GTTAATTAAT
    TCTCTGCCAT TTACATCTCA TGGCAAAATT GATGTTTCTG AGTTAAATAA GATATATTTA
    AACTACAGAG ACTTGAAGTC TGAGTGTAAG CTCAGTGGAA AAGAGGAACT TTGGGAAAAA
    TTACACCATT TGTGGAAGTC TGTTCTGAGT CTCTCAGAAG ATGCTTTGAA GATTCCTGAT
    GAGTCACTCT TCTTAAATAG TGGTGGAGAT TCCTTGAAAT CCATACGGCT TCTCAATGAG
    ATTGAAAACC TTGTTGGCAC ATCAGTACCT GGGCTTCTGG AAATTATTCT CAGCAGTTCT
    ATTTTACGGA TTTACAATCA CATCCTTCAA ACAGTGTTTT CAGATAAAGA TATGACACTT
    AGCAAGAATC ATGCCATGAA AAGAAAATTC AACGTTACTC AAGAGGAAAC CAGTGGAAAA
    TCTTTACATC AGGAAACTGT CATGCCTTTA AATTGTGACA AGGAGATCAA TGCTTTTATT
    GCACTGAGCA GAGGGAATCA GATTTTGTCT TTGAATACTG ATAAGTATTT AACTAAGTTG
    GGACATTGCC CTTCAACCTA TTCTTCTGAT TTAAATTCAC AGACTGACAT TCAAAATAGA
    GAAAGCCTAA ATACTCCACC TCTTATTGGG AAGTCAGAAG ATCCATCCTG TGTTGTGGAA
    GTTTCTGAAG AGGAAACACC TGTGATTAGG GCTGAGAAAA TGGAGTTTCG TGTGAGGTGG
    AGGTCAGACA CAGGCAAATG TGTGGATGCT TCACCTCTGG TTGTAATACC AGCTATTGAT
    AAGTCATCTG CAACTGTGTA CATTGGCTCT CATTCTCATA GAATGATAGC AGTTGATCTT
    TACTCTGGAA AGGTGAAATG GGAACAGATT TTAGGAGATC GCATTGAATC CTCAGCATGT
    GTATCTAAGT GTGGAAACTT TATTGTAGTG GGCTGTTATA ATGGGTTAGT TTATGTTCTG
    AAAAGTAATA GTGGAGAAAA ATACTGGATG TTTAGTACTG AGGATGCTGT CAAAAGCTCA
    GCAACCATGG ATCCAACCAC AGGACTTCTT TACATTGGAT CTCATGACCA GCATGCATAC
    GCTTTGGATA TATATAAGAA GAAGTGTGTT TGGAAGTTGA AATGTGGAGG AACTGTTTTT
    TCTTCTCCTT GTTTGAGCCT GATTCCATCT CACTTGTATT TTGCTACACT GGGAGGACTT
    TTACTGGCTA TAAATCCTGC CACTGGGGAC AGACTTTGGA AACATTCCTG TGGAAAGCCA
    CTCTTTTCTT CTCCACGGTG TTGCCTACAG TATGTTTTTA TTGGCTGTGT AGATGGAAAT
    TTACTCTGCT TTACTCACTT TGGAGAACAG GTTTGGCAAT TCTCTACCAA TGAACCAATC
    TTTTCATCCC CATGTACCTC AGCATCAGAG CAAGAAATAT TTTTTGGTTC TCATGACTGC
    TTTATCTACT GTTGTAGTAT GAAAGGTCAC CTCCAGTGGA AATTTGAAAC TACTTCAAGA
    GTGTATTCAA CGCCTTTTGT TTTCCGTAAC CACAACCATA ACAGTGAAAT GTTACTGGCA
    GCGGCATCTA CTGATGGGAA ACTGTGGATC TTGGAATCTA AGAGTGGACA GTTGCAAAGT
    GTGTATGAAC TTCCTGGAGA AGTTTTCTCT TCTCCTGTGG TCTGGGAATC AATGCTTATT
    ATTGGATGTA GAAATAATTA TGTTTATTGT CTGGATGTAT TGGGTGACAA AGGTAATGAA
    GTGCAGTCCT TAGAATATAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849345.1
    CDS450..3413
    Translation

    Target ORF information:

    RefSeq Version XM_024993577.1
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993577.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTA AATTCAAGTC TTCCTATGAA GCATTATTGA ACTATGATAT GCTTAAAGTA
    GAACATAATG ACCTAGTACT CTTCAGACTT CACTGGAAAA ATGTCGAGGT GAGCCTGGTG
    CTAAATGATA GAAAAGAGAA ATATGAAAAA GGAAAGATGC CAAAGAGTAT GCATTCTGAG
    AGCAGCAGTG AAGAAAAGTC AGAAGAGCAC ATGGATATGA GGCAAAAGCA CTGCTTGGCC
    TATGTTCTCC ATACGTCAGG CACTACAGGG ATACCTAAGA TTGTCAGAGT CCCTCATGCA
    TGTATAGTGC CAAACATCCA GCATTTTCGA TGTGAATTGC CTGTACAACT GGGATTCCCA
    CTGCTTGGAA CAGTAGTTGA AGTCAGAGAT ACTAACGGTT TCACAATTCA AGAAGGCAAT
    GGCCAAGTAT TTTTAGGTGG CAGAAATAGA GTATGTTTTC TCGATGGCGA AGTGACGGTA
    CCACTTGGCA CAATGAGAGC CACAGGAGAC TTTGTGACTA TAAAAGATGG AGAGATATTT
    TTCTTGGGAC GAAAGGACAG TCAGATCAAA CGTCATGGCA AACGTCTTAA CATAGAACTT
    GTACAACAGA TTGCTGAAGG TCTTCAACAA GTGGAGTCTT GTGCAGTTAC ATGGTATAAC
    CAGGAAAAAT TGATTTTGTT CATGGTGACT AAAAATGATT TAGTAAAGGA TCACATCTTT
    GAAGAACTGC AGAAACATCT TCCAAGTCAT GCCATCCCAG ATGAGCTTGT GTTAATTAAT
    TCTCTGCCAT TTACATCTCA TGGCAAAATT GATGTTTCTG AGTTAAATAA GATATATTTA
    AACTACAGAG ACTTGAAGTC TGAGTGTAAG CTCAGTGGAA AAGAGGAACT TTGGGAAAAA
    TTACACCATT TGTGGAAGTC TGTTCTGAGT CTCTCAGAAG ATGCTTTGAA GATTCCTGAT
    GAGTCACTCT TCTTAAATAG TGGTGGAGAT TCCTTGAAAT CCATACGGCT TCTCAATGAG
    ATTGAAAACC TTGTTGGCAC ATCAGTACCT GGGCTTCTGG AAATTATTCT CAGCAGTTCT
    ATTTTACGGA TTTACAATCA CATCCTTCAA ACAGTGTTTT CAGATAAAGA TATGACACTT
    AGCAAGAATC ATGCCATGAA AAGAAAATTC AACGTTACTC AAGAGGAAAC CAGTGGAAAA
    TCTTTACATC AGGAAACTGT CATGCCTTTA AATTGTGACA AGGAGATCAA TGCTTTTATT
    GCACTGAGCA GAGGGAATCA GATTTTGTCT TTGAATACTG ATAAGTATTT AACTAAGTTG
    GGACATTGCC CTTCAACCTA TTCTTCTGAT TTAAATTCAC AGACTGACAT TCAAAATAGA
    GAAAGCCTAA ATACTCCACC TCTTATTGGG AAGTCAGAAG ATCCATCCTG TGTTGTGGAA
    GTTTCTGAAG AGGAAACACC TGTGATTAGG GCTGAGAAAA TGGAGTTTCG TGTGAGGTGG
    AGGTCAGACA CAGGCAAATG TGTGGATGCT TCACCTCTGG TTGTAATACC AGCTATTGAT
    AAGTCATCTG CAACTGTGTA CATTGGCTCT CATTCTCATA GAATGATAGC AGTTGATCTT
    TACTCTGGAA AGGTGAAATG GGAACAGATT TTAGGAGATC GCATTGAATC CTCAGCATGT
    GTATCTAAGT GTGGAAACTT TATTGTAGTG GGCTGTTATA ATGGGTTAGT TTATGTTCTG
    AAAAGTAATA GTGGAGAAAA ATACTGGATG TTTAGTACTG AGGATGCTGT CAAAAGCTCA
    GCAACCATGG ATCCAACCAC AGGACTTCTT TACATTGGAT CTCATGACCA GCATGCATAC
    GCTTTGGATA TATATAAGAA GAAGTGTGTT TGGAAGTTGA AATGTGGAGG AACTGTTTTT
    TCTTCTCCTT GTTTGAGCCT GATTCCATCT CACTTGTATT TTGCTACACT GGGAGGACTT
    TTACTGGCTA TAAATCCTGC CACTGGGGAC AGACTTTGGA AACATTCCTG TGGAAAGCCA
    CTCTTTTCTT CTCCACGGTG TTGCCTACAG TATGTTTTTA TTGGCTGTGT AGATGGAAAT
    TTACTCTGCT TTACTCACTT TGGAGAACAG GTTTGGCAAT TCTCTACCAA TGAACCAATC
    TTTTCATCCC CATGTACCTC AGCATCAGAG CAAGAAATAT TTTTTGGTTC TCATGACTGC
    TTTATCTACT GTTGTAGTAT GAAAGGTCAC CTCCAGTGGA AATTTGAAAC TACTTCAAGA
    GTGTATTCAA CGCCTTTTGT TTTCCGTAAC CACAACCATA ACAGTGAAAT GTTACTGGCA
    GCGGCATCTA CTGATGGGAA ACTGTGGATC TTGGAATCTA AGAGTGGACA GTTGCAAAGT
    GTGTATGAAC TTCCTGGAGA AGTTTTCTCT TCTCCTGTGG TCTGGGAATC AATGCTTATT
    ATTGGATGTA GAAATAATTA TGTTTATTGT CTGGATGTAT TGGGTGACAA AGGTAATGAA
    GTGCAGTCCT TAGAATATAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235567
    Clone ID Related Accession (Same CDS sequence) XM_024993578.1
    Accession Version XM_024993578.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product beta-alanine-activating enzyme isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAAAAAT GTAATCTGAA GTATATCCTT GTTGAAAAAG TGCATATAAG TAAATTCAAG 
    TCTTCCTATG AAGCATTATT GAACTATGAT ATGCTTAAAG TAGAACATAA TGACCTAGTA
    CTCTTCAGAC TTCACTGGAA AAATGTCGAG GTGAGCCTGG TGCTAAATGA TAGAAAAGAG
    AAATATGAAA AAGGAAAGAT GCCAAAGAGT ATGCATTCTG AGAGCAGCAG TGAAGAAAAG
    TCAGAAGAGC ACATGGATAT GAGGCAAAAG CACTGCTTGG CCTATGTTCT CCATACGTCA
    GGCACTACAG GGATACCTAA GATTGTCAGA GTCCCTCATG CATGTATAGT GCCAAACATC
    CAGCATTTTC GGGTGCTTTT TGAAATCACT CATGAGGATG TTTTGTTTCT GGCTTCACCT
    CTGACCTTTG ATCCTTCTGT GGTGGAAATA TTTGTTGCTT TGTCAAGTGG TGCCTCTTTG
    CTTATTGTGC CAACTTCTGT CAAAATGGTC CCATCAAAAT TAGCTGCTGT TCTGTTTTCC
    CATCACAGAG TGACTATTTT ACAGGCAACG CCAACATTGC TTAGAAGATT TGGATCTCAG
    CTCATCAAGT CAACTGTTTT ATCCGCCAGT ACCTCTCTTC GCATATTGGC CCTTGGTGGT
    GAAGCATTTC CATCATTAAC TGTTCTCAAA AGCTGGAGAG GAGTGGGCAA TAAAACACAA
    ATATTTAATA TTTATGGCAT CACAGAGGTA TCAAGTTGGG CGACTTTCTA CAGGATTCCA
    GAGAAGATTC TTAACTCTAC TTTGAAATGT GAATTGCCTG TACAACTGGG ATTCCCACTG
    CTTGGAACAG TAGTTGAAGT CAGAGATACT AACGGTTTCA CAATTCAAGA AGGCAATGGC
    CAAGTATTTT TAGGTGGCAG AAATAGAGTA TGTTTTCTCG ATGGCGAAGT GACGGTACCA
    CTTGGCACAA TGAGAGCCAC AGGAGACTTT GTGACTATAA AAGATGGAGA GATATTTTTC
    TTGGGACGAA AGGACAGTCA GATCAAACGT CATGGCAAAC GTCTTAACAT AGAACTTGTA
    CAACAGATTG CTGAAGGTCT TCAACAAGTG GAGTCTTGTG CAGTTACATG GTATAACCAG
    GAAAAATTGA TTTTGTTCAT GGTGACTAAA AATGATTTAG TAAAGGATCA CATCTTTGAA
    GAACTGCAGA AACATCTTCC AAGTCATGCC ATCCCAGATG AGCTTGTGTT AATTAATTCT
    CTGCCATTTA CATCTCATGG CAAAATTGAT GTTTCTGAGT TAAATAAGAT ATATTTAAAC
    TACAGAGACT TGAAGTCTGA GTGTAAGCTC AGTGGAAAAG AGGAACTTTG GGAAAAATTA
    CACCATTTGT GGAAGTCTGT TCTGAGTCTC TCAGAAGATG CTTTGAAGAT TCCTGATGAG
    TCACTCTTCT TAAATAGTGG TGGAGATTCC TTGAAATCCA TACGGCTTCT CAATGAGATT
    GAAAACCTTG TTGGCACATC AGTACCTGGG CTTCTGGAAA TTATTCTCAG CAGTTCTATT
    TTACGGATTT ACAATCACAT CCTTCAAACA GTGTTTTCAG ATAAAGATAT GACACTTAGC
    AAGAATCATG CCATGAAAAG AAAATTCAAC GTTACTCAAG AGGAAACCAG TGGAAAATCT
    TTACATCAGG AAACTGTCAT GCCTTTAAAT TGTGACAAGG AGATCAATGC TTTTATTGCA
    CTGAGCAGAG GGAATCAGAT TTTGTCTTTG AATACTGATA AGTATTTAAC TAAGTTGGGA
    CATTGCCCTT CAACCTATTC TTCTGATTTA AATTCACAGA CTGACATTCA AAATAGAGAA
    AGCCTAAATA CTCCACCTCT TATTGGGAAG TCAGAAGATC CATCCTGTGT TGTGGAAGTT
    TCTGAAGAGG AAACACCTGT GATTAGGGCT GAGAAAATGG AGTTTCGTGT GAGGTGGAGG
    TCAGACACAG GCAAATGTGT GGATGCTTCA CCTCTGGTTG TAATACCAGC TATTGATAAG
    TCATCTGCAA CTGTGTACAT TGGCTCTCAT TCTCATAGAA TGATAGCAGT TGATCTTTAC
    TCTGGAAAGG TGAAATGGGA ACAGATTTTA GGAGATCGCA TTGAATCCTC AGCATGTGTA
    TCTAAGTGTG GAAACTTTAT TGTAGTGGGC TGTTATAATG GGTTAGTTTA TGTTCTGAAA
    AGTAATAGTG GAGAAAAATA CTGGATGTTT AGTACTGAGG ATGCTGTCAA AAGCTCAGCA
    ACCATGGATC CAACCACAGG ACTTCTTTAC ATTGGATCTC ATGACCAGCA TGCATACGCT
    TTGGATATAT ATGCCACTGG GGACAGACTT TGGAAACATT CCTGTGGAAA GCCACTCTTT
    TCTTCTCCAC GGTGTTGCCT ACAGTATGTT TTTATTGGCT GTGTAGATGG AAATTTACTC
    TGCTTTACTC ACTTTGGAGA ACAGGTTTGG CAATTCTCTA CCAATGAACC AATCTTTTCA
    TCCCCATGTA CCTCAGCATC AGAGCAAGAA ATATTTTTTG GTTCTCATGA CTGCTTTATC
    TACTGTTGTA GTATGAAAGG TCACCTCCAG TGGAAATTTG AAACTACTTC AAGAGTGTAT
    TCAACGCCTT TTGTTTTCCG TAACCACAAC CATAACAGTG AAATGTTACT GGCAGCGGCA
    TCTACTGATG GGAAACTGTG GATCTTGGAA TCTAAGAGTG GACAGTTGCA AAGTGTGTAT
    GAACTTCCTG GAGAAGTTTT CTCTTCTCCT GTGGTCTGGG AATCAATGCT TATTATTGGA
    TGTAGAAATA ATTATGTTTA TTGTCTGGAT GTATTGGGTG ACAAAGGTAA TGAAGTGCAG
    TCCTTAGAAT ATACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849346.1
    CDS559..3456
    Translation

    Target ORF information:

    RefSeq Version XM_024993578.1
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993578.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAAAAAT GTAATCTGAA GTATATCCTT GTTGAAAAAG TGCATATAAG TAAATTCAAG 
    TCTTCCTATG AAGCATTATT GAACTATGAT ATGCTTAAAG TAGAACATAA TGACCTAGTA
    CTCTTCAGAC TTCACTGGAA AAATGTCGAG GTGAGCCTGG TGCTAAATGA TAGAAAAGAG
    AAATATGAAA AAGGAAAGAT GCCAAAGAGT ATGCATTCTG AGAGCAGCAG TGAAGAAAAG
    TCAGAAGAGC ACATGGATAT GAGGCAAAAG CACTGCTTGG CCTATGTTCT CCATACGTCA
    GGCACTACAG GGATACCTAA GATTGTCAGA GTCCCTCATG CATGTATAGT GCCAAACATC
    CAGCATTTTC GGGTGCTTTT TGAAATCACT CATGAGGATG TTTTGTTTCT GGCTTCACCT
    CTGACCTTTG ATCCTTCTGT GGTGGAAATA TTTGTTGCTT TGTCAAGTGG TGCCTCTTTG
    CTTATTGTGC CAACTTCTGT CAAAATGGTC CCATCAAAAT TAGCTGCTGT TCTGTTTTCC
    CATCACAGAG TGACTATTTT ACAGGCAACG CCAACATTGC TTAGAAGATT TGGATCTCAG
    CTCATCAAGT CAACTGTTTT ATCCGCCAGT ACCTCTCTTC GCATATTGGC CCTTGGTGGT
    GAAGCATTTC CATCATTAAC TGTTCTCAAA AGCTGGAGAG GAGTGGGCAA TAAAACACAA
    ATATTTAATA TTTATGGCAT CACAGAGGTA TCAAGTTGGG CGACTTTCTA CAGGATTCCA
    GAGAAGATTC TTAACTCTAC TTTGAAATGT GAATTGCCTG TACAACTGGG ATTCCCACTG
    CTTGGAACAG TAGTTGAAGT CAGAGATACT AACGGTTTCA CAATTCAAGA AGGCAATGGC
    CAAGTATTTT TAGGTGGCAG AAATAGAGTA TGTTTTCTCG ATGGCGAAGT GACGGTACCA
    CTTGGCACAA TGAGAGCCAC AGGAGACTTT GTGACTATAA AAGATGGAGA GATATTTTTC
    TTGGGACGAA AGGACAGTCA GATCAAACGT CATGGCAAAC GTCTTAACAT AGAACTTGTA
    CAACAGATTG CTGAAGGTCT TCAACAAGTG GAGTCTTGTG CAGTTACATG GTATAACCAG
    GAAAAATTGA TTTTGTTCAT GGTGACTAAA AATGATTTAG TAAAGGATCA CATCTTTGAA
    GAACTGCAGA AACATCTTCC AAGTCATGCC ATCCCAGATG AGCTTGTGTT AATTAATTCT
    CTGCCATTTA CATCTCATGG CAAAATTGAT GTTTCTGAGT TAAATAAGAT ATATTTAAAC
    TACAGAGACT TGAAGTCTGA GTGTAAGCTC AGTGGAAAAG AGGAACTTTG GGAAAAATTA
    CACCATTTGT GGAAGTCTGT TCTGAGTCTC TCAGAAGATG CTTTGAAGAT TCCTGATGAG
    TCACTCTTCT TAAATAGTGG TGGAGATTCC TTGAAATCCA TACGGCTTCT CAATGAGATT
    GAAAACCTTG TTGGCACATC AGTACCTGGG CTTCTGGAAA TTATTCTCAG CAGTTCTATT
    TTACGGATTT ACAATCACAT CCTTCAAACA GTGTTTTCAG ATAAAGATAT GACACTTAGC
    AAGAATCATG CCATGAAAAG AAAATTCAAC GTTACTCAAG AGGAAACCAG TGGAAAATCT
    TTACATCAGG AAACTGTCAT GCCTTTAAAT TGTGACAAGG AGATCAATGC TTTTATTGCA
    CTGAGCAGAG GGAATCAGAT TTTGTCTTTG AATACTGATA AGTATTTAAC TAAGTTGGGA
    CATTGCCCTT CAACCTATTC TTCTGATTTA AATTCACAGA CTGACATTCA AAATAGAGAA
    AGCCTAAATA CTCCACCTCT TATTGGGAAG TCAGAAGATC CATCCTGTGT TGTGGAAGTT
    TCTGAAGAGG AAACACCTGT GATTAGGGCT GAGAAAATGG AGTTTCGTGT GAGGTGGAGG
    TCAGACACAG GCAAATGTGT GGATGCTTCA CCTCTGGTTG TAATACCAGC TATTGATAAG
    TCATCTGCAA CTGTGTACAT TGGCTCTCAT TCTCATAGAA TGATAGCAGT TGATCTTTAC
    TCTGGAAAGG TGAAATGGGA ACAGATTTTA GGAGATCGCA TTGAATCCTC AGCATGTGTA
    TCTAAGTGTG GAAACTTTAT TGTAGTGGGC TGTTATAATG GGTTAGTTTA TGTTCTGAAA
    AGTAATAGTG GAGAAAAATA CTGGATGTTT AGTACTGAGG ATGCTGTCAA AAGCTCAGCA
    ACCATGGATC CAACCACAGG ACTTCTTTAC ATTGGATCTC ATGACCAGCA TGCATACGCT
    TTGGATATAT ATGCCACTGG GGACAGACTT TGGAAACATT CCTGTGGAAA GCCACTCTTT
    TCTTCTCCAC GGTGTTGCCT ACAGTATGTT TTTATTGGCT GTGTAGATGG AAATTTACTC
    TGCTTTACTC ACTTTGGAGA ACAGGTTTGG CAATTCTCTA CCAATGAACC AATCTTTTCA
    TCCCCATGTA CCTCAGCATC AGAGCAAGAA ATATTTTTTG GTTCTCATGA CTGCTTTATC
    TACTGTTGTA GTATGAAAGG TCACCTCCAG TGGAAATTTG AAACTACTTC AAGAGTGTAT
    TCAACGCCTT TTGTTTTCCG TAACCACAAC CATAACAGTG AAATGTTACT GGCAGCGGCA
    TCTACTGATG GGAAACTGTG GATCTTGGAA TCTAAGAGTG GACAGTTGCA AAGTGTGTAT
    GAACTTCCTG GAGAAGTTTT CTCTTCTCCT GTGGTCTGGG AATCAATGCT TATTATTGGA
    TGTAGAAATA ATTATGTTTA TTGTCTGGAT GTATTGGGTG ACAAAGGTAA TGAAGTGCAG
    TCCTTAGAAT ATACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235568
    Clone ID Related Accession (Same CDS sequence) XM_024993579.1
    Accession Version XM_024993579.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2886bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product beta-alanine-activating enzyme isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTG CAACGCCAAC ATTGCTTAGA AGATTTGGAT CTCAGCTCAT CAAGTCAACT
    GTTTTATCCG CCAGTACCTC TCTTCGCATA TTGGCCCTTG GTGGTGAAGC ATTTCCATCA
    TTAACTGTTC TCAAAAGCTG GAGAGGAGTG GGCAATAAAA CACAAATATT TAATATTTAT
    GGCATCACAG AGGTATCAAG TTGGGCGACT TTCTACAGGA TTCCAGAGAA GATTCTTAAC
    TCTACTTTGA AATGTGAATT GCCTGTACAA CTGGGATTCC CACTGCTTGG AACAGTAGTT
    GAAGTCAGAG ATACTAACGG TTTCACAATT CAAGAAGGCA ATGGCCAAGT ATTTTTAGGT
    GGCAGAAATA GAGTATGTTT TCTCGATGGC GAAGTGACGG TACCACTTGG CACAATGAGA
    GCCACAGGAG ACTTTGTGAC TATAAAAGAT GGAGAGATAT TTTTCTTGGG ACGAAAGGAC
    AGTCAGATCA AACGTCATGG CAAACGTCTT AACATAGAAC TTGTACAACA GATTGCTGAA
    GGTCTTCAAC AAGTGGAGTC TTGTGCAGTT ACATGGTATA ACCAGGAAAA ATTGATTTTG
    TTCATGGTGA CTAAAAATGA TTTAGTAAAG GATCACATCT TTGAAGAACT GCAGAAACAT
    CTTCCAAGTC ATGCCATCCC AGATGAGCTT GTGTTAATTA ATTCTCTGCC ATTTACATCT
    CATGGCAAAA TTGATGTTTC TGAGTTAAAT AAGATATATT TAAACTACAG AGACTTGAAG
    TCTGAGTGTA AGCTCAGTGG AAAAGAGGAA CTTTGGGAAA AATTACACCA TTTGTGGAAG
    TCTGTTCTGA GTCTCTCAGA AGATGCTTTG AAGATTCCTG ATGAGTCACT CTTCTTAAAT
    AGTGGTGGAG ATTCCTTGAA ATCCATACGG CTTCTCAATG AGATTGAAAA CCTTGTTGGC
    ACATCAGTAC CTGGGCTTCT GGAAATTATT CTCAGCAGTT CTATTTTACG GATTTACAAT
    CACATCCTTC AAACAGTGTT TTCAGATAAA GATATGACAC TTAGCAAGAA TCATGCCATG
    AAAAGAAAAT TCAACGTTAC TCAAGAGGAA ACCAGTGGAA AATCTTTACA TCAGGAAACT
    GTCATGCCTT TAAATTGTGA CAAGGAGATC AATGCTTTTA TTGCACTGAG CAGAGGGAAT
    CAGATTTTGT CTTTGAATAC TGATAAGTAT TTAACTAAGT TGGGACATTG CCCTTCAACC
    TATTCTTCTG ATTTAAATTC ACAGACTGAC ATTCAAAATA GAGAAAGCCT AAATACTCCA
    CCTCTTATTG GGAAGTCAGA AGATCCATCC TGTGTTGTGG AAGTTTCTGA AGAGGAAACA
    CCTGTGATTA GGGCTGAGAA AATGGAGTTT CGTGTGAGGT GGAGGTCAGA CACAGGCAAA
    TGTGTGGATG CTTCACCTCT GGTTGTAATA CCAGCTATTG ATAAGTCATC TGCAACTGTG
    TACATTGGCT CTCATTCTCA TAGAATGATA GCAGTTGATC TTTACTCTGG AAAGGTGAAA
    TGGGAACAGA TTTTAGGAGA TCGCATTGAA TCCTCAGCAT GTGTATCTAA GTGTGGAAAC
    TTTATTGTAG TGGGCTGTTA TAATGGGTTA GTTTATGTTC TGAAAAGTAA TAGTGGAGAA
    AAATACTGGA TGTTTAGTAC TGAGGATGCT GTCAAAAGCT CAGCAACCAT GGATCCAACC
    ACAGGACTTC TTTACATTGG ATCTCATGAC CAGCATGCAT ACGCTTTGGA TATATATAAG
    AAGAAGTGTG TTTGGAAGTT GAAATGTGGA GGAACTGTTT TTTCTTCTCC TTGTTTGAGC
    CTGATTCCAT CTCACTTGTA TTTTGCTACA CTGGGAGGAC TTTTACTGGC TATAAATCCT
    GCCACTGGGG ACAGACTTTG GAAACATTCC TGTGGAAAGC CACTCTTTTC TTCTCCACGG
    TGTTGCCTAC AGTATGTTTT TATTGGCTGT GTAGATGGAA ATTTACTCTG CTTTACTCAC
    TTTGGAGAAC AGGTTTGGCA ATTCTCTACC AATGAACCAA TCTTTTCATC CCCATGTACC
    TCAGCATCAG AGCAAGAAAT ATTTTTTGGT TCTCATGACT GCTTTATCTA CTGTTGTAGT
    ATGAAAGGTC ACCTCCAGTG GAAATTTGAA ACTACTTCAA GAGTGTATTC AACGCCTTTT
    GTTTTCCGTA ACCACAACCA TAACAGTGAA ATGTTACTGG CAGCGGCATC TACTGATGGG
    AAACTGTGGA TCTTGGAATC TAAGAGTGGA CAGTTGCAAA GTGTGTATGA ACTTCCTGGA
    GAAGTTTTCT CTTCTCCTGT GGTCTGGGAA TCAATGCTTA TTATTGGATG TAGAAATAAT
    TATGTTTATT GTCTGGATGT ATTGGGTGAC AAAGGTAATG AAGTGCAGTC CTTAGAATAT
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849347.1
    CDS449..3334
    Translation

    Target ORF information:

    RefSeq Version XM_024993579.1
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993579.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTG CAACGCCAAC ATTGCTTAGA AGATTTGGAT CTCAGCTCAT CAAGTCAACT
    GTTTTATCCG CCAGTACCTC TCTTCGCATA TTGGCCCTTG GTGGTGAAGC ATTTCCATCA
    TTAACTGTTC TCAAAAGCTG GAGAGGAGTG GGCAATAAAA CACAAATATT TAATATTTAT
    GGCATCACAG AGGTATCAAG TTGGGCGACT TTCTACAGGA TTCCAGAGAA GATTCTTAAC
    TCTACTTTGA AATGTGAATT GCCTGTACAA CTGGGATTCC CACTGCTTGG AACAGTAGTT
    GAAGTCAGAG ATACTAACGG TTTCACAATT CAAGAAGGCA ATGGCCAAGT ATTTTTAGGT
    GGCAGAAATA GAGTATGTTT TCTCGATGGC GAAGTGACGG TACCACTTGG CACAATGAGA
    GCCACAGGAG ACTTTGTGAC TATAAAAGAT GGAGAGATAT TTTTCTTGGG ACGAAAGGAC
    AGTCAGATCA AACGTCATGG CAAACGTCTT AACATAGAAC TTGTACAACA GATTGCTGAA
    GGTCTTCAAC AAGTGGAGTC TTGTGCAGTT ACATGGTATA ACCAGGAAAA ATTGATTTTG
    TTCATGGTGA CTAAAAATGA TTTAGTAAAG GATCACATCT TTGAAGAACT GCAGAAACAT
    CTTCCAAGTC ATGCCATCCC AGATGAGCTT GTGTTAATTA ATTCTCTGCC ATTTACATCT
    CATGGCAAAA TTGATGTTTC TGAGTTAAAT AAGATATATT TAAACTACAG AGACTTGAAG
    TCTGAGTGTA AGCTCAGTGG AAAAGAGGAA CTTTGGGAAA AATTACACCA TTTGTGGAAG
    TCTGTTCTGA GTCTCTCAGA AGATGCTTTG AAGATTCCTG ATGAGTCACT CTTCTTAAAT
    AGTGGTGGAG ATTCCTTGAA ATCCATACGG CTTCTCAATG AGATTGAAAA CCTTGTTGGC
    ACATCAGTAC CTGGGCTTCT GGAAATTATT CTCAGCAGTT CTATTTTACG GATTTACAAT
    CACATCCTTC AAACAGTGTT TTCAGATAAA GATATGACAC TTAGCAAGAA TCATGCCATG
    AAAAGAAAAT TCAACGTTAC TCAAGAGGAA ACCAGTGGAA AATCTTTACA TCAGGAAACT
    GTCATGCCTT TAAATTGTGA CAAGGAGATC AATGCTTTTA TTGCACTGAG CAGAGGGAAT
    CAGATTTTGT CTTTGAATAC TGATAAGTAT TTAACTAAGT TGGGACATTG CCCTTCAACC
    TATTCTTCTG ATTTAAATTC ACAGACTGAC ATTCAAAATA GAGAAAGCCT AAATACTCCA
    CCTCTTATTG GGAAGTCAGA AGATCCATCC TGTGTTGTGG AAGTTTCTGA AGAGGAAACA
    CCTGTGATTA GGGCTGAGAA AATGGAGTTT CGTGTGAGGT GGAGGTCAGA CACAGGCAAA
    TGTGTGGATG CTTCACCTCT GGTTGTAATA CCAGCTATTG ATAAGTCATC TGCAACTGTG
    TACATTGGCT CTCATTCTCA TAGAATGATA GCAGTTGATC TTTACTCTGG AAAGGTGAAA
    TGGGAACAGA TTTTAGGAGA TCGCATTGAA TCCTCAGCAT GTGTATCTAA GTGTGGAAAC
    TTTATTGTAG TGGGCTGTTA TAATGGGTTA GTTTATGTTC TGAAAAGTAA TAGTGGAGAA
    AAATACTGGA TGTTTAGTAC TGAGGATGCT GTCAAAAGCT CAGCAACCAT GGATCCAACC
    ACAGGACTTC TTTACATTGG ATCTCATGAC CAGCATGCAT ACGCTTTGGA TATATATAAG
    AAGAAGTGTG TTTGGAAGTT GAAATGTGGA GGAACTGTTT TTTCTTCTCC TTGTTTGAGC
    CTGATTCCAT CTCACTTGTA TTTTGCTACA CTGGGAGGAC TTTTACTGGC TATAAATCCT
    GCCACTGGGG ACAGACTTTG GAAACATTCC TGTGGAAAGC CACTCTTTTC TTCTCCACGG
    TGTTGCCTAC AGTATGTTTT TATTGGCTGT GTAGATGGAA ATTTACTCTG CTTTACTCAC
    TTTGGAGAAC AGGTTTGGCA ATTCTCTACC AATGAACCAA TCTTTTCATC CCCATGTACC
    TCAGCATCAG AGCAAGAAAT ATTTTTTGGT TCTCATGACT GCTTTATCTA CTGTTGTAGT
    ATGAAAGGTC ACCTCCAGTG GAAATTTGAA ACTACTTCAA GAGTGTATTC AACGCCTTTT
    GTTTTCCGTA ACCACAACCA TAACAGTGAA ATGTTACTGG CAGCGGCATC TACTGATGGG
    AAACTGTGGA TCTTGGAATC TAAGAGTGGA CAGTTGCAAA GTGTGTATGA ACTTCCTGGA
    GAAGTTTTCT CTTCTCCTGT GGTCTGGGAA TCAATGCTTA TTATTGGATG TAGAAATAAT
    TATGTTTATT GTCTGGATGT ATTGGGTGAC AAAGGTAATG AAGTGCAGTC CTTAGAATAT
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235569
    Clone ID Related Accession (Same CDS sequence) XM_024993580.1
    Accession Version XM_024993580.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2487bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product beta-alanine-activating enzyme isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTATAGTG CCAAACATCC AGCATTTTCG GCAACGCCAA CATTGCTTAG AAGATTTGGA 
    TCTCAGCTCA TCAAGTCAAC TGTTTTATCC GCCAGTACCT CTCTTCGCAT ATTGGCCCTT
    GGTGGTGAAG CATTTCCATC ATTAACTGTT CTCAAAAGCT GGAGAGGAGT GGGCAATAAA
    ACACAAATAT TTAATATTTA TGGCATCACA GAGGTATCAA GTTGGGCGAC TTTCTACAGG
    ATTCCAGAGA AGATTCTTAA CTCTACTTTG AAATGTGAAT TGCCTGTACA ACTGGGATTC
    CCACTGCTTG GAACAGTAGT TGAAGTCAGA GATACTAACG GTTTCACAAT TCAAGAAGGC
    AATGGCCAAG TATTTTTAGG TGGCAGAAAT AGAGTATGTT TTCTCGATGG CGAAGTGACG
    GTACCACTTG GCACAATGAG AGCCACAGGA GACTTTGTGA CTATAAAAGA TGGAGAGATA
    TTTTTCTTGG GACGAAAGGA CAGTCAGATC AAACGTCATG GCAAACGTCT TAACATAGAA
    CTTGTACAAC AGATTGCTGA AGGTCTTCAA CAAGTGGAGT CTTGTGCAGT TACATGGTAT
    AACCAGGAAA AATTGATTTT GTTCATGGTG ACTAAAAATG ATTTAGTAAA GGATCACATC
    TTTGAAGAAC TGCAGAAACA TCTTCCAAGT CATGCCATCC CAGATGAGCT TGTGTTAATT
    AATTCTCTGC CATTTACATC TCATGGCAAA ATTGATGTTT CTGAGTTAAA TAAGATATAT
    TTAAACTACA GAGACTTGAA GTCTGAGTGT AAGCTCAGTG GAAAAGAGGA ACTTTGGGAA
    AAATTACACC ATTTGTGGAA GTCTGTTCTG AGTCTCTCAG AAGATGCTTT GAAGATTCCT
    GATGAGTCAC TCTTCTTAAA TAGTGGTGGA GATTCCTTGA AATCCATACG GCTTCTCAAT
    GAGATTGAAA ACCTTGTTGG CACATCAGTA CCTGGGCTTC TGGAAATTAT TCTCAGCAGT
    TCTATTTTAC GGATTTACAA TCACATCCTT CAAACAGTGT TTTCAGATAA AGATATGACA
    CTTAGCAAGA ATCATGCCAT GAAAAGAAAA TTCAACGTTA CTCAAGAGGA AACCAGTGGA
    AAATCTTTAC ATCAGGAAAC TGTCATGCCT TTAAATTGTG ACAAGGAGAT CAATGCTTTT
    ATTGCACTGA GCAGAGGGAA TCAGATTTTG TCTTTGAATA CTGATAAGTA TTTAACTAAG
    TTGGGACATT GCCCTTCAAC CTATTCTTCT GATTTAAATT CACAGACTGA CATTCAAAAT
    AGAGAAAGCC TAAATACTCC ACCTCTTATT GGGAAGTCAG AAGATCCATC CTGTGTTGTG
    GAAGTTTCTG AAGAGGAAAC ACCTGTGATT AGGGCTGAGA AAATGGAGTT TCGTGTGAGG
    TGGAGGTCAG ACACAGGCAA ATGTGTGGAT GCTTCACCTC TGGTTGTAAT ACCAGCTATT
    GATAAGTCAT CTGCAACTGT GTACATTGGC TCTCATTCTC ATAGAATGAT AGCAGTTGAT
    CTTTACTCTG GAAAGGTGAA ATGGGAACAG ATTTTAGGAG ATCGCATTGA ATCCTCAGCA
    TGTGTATCTA AGTGTGGAAA CTTTATTGTA GTGGGCTGTT ATAATGGGTT AGTTTATGTT
    CTGAAAAGTA ATAGTGGAGA AAAATACTGG ATGTTTAGTA CTGAGGATGC TGTCAAAAGC
    TCAGCAACCA TGGATCCAAC CACAGGACTT CTTTACATTG GATCTCATGA CCAGCATGCA
    TACGCTTTGG ATATATATAA GAAGAAGTGT GTTTGGAAGT TGAAATGTGG AGGAACTGTT
    TTTTCTTCTC CTTGTTTGAG CCTGATTCCA TCTCACTTGT ATTTTGCTAC ACTGGGAGGA
    CTTTTACTGG CTATAAATCC TGCCACTGGG GACAGACTTT GGAAACATTC CTGTGGAAAG
    CCACTCTTTT CTTCTCCACG GTGTTGCCTA CAGTATGTTT TTATTGGCTG TGTAGATGGA
    AATTTACTCT GCTTTACTCA CTTTGGAGAA CAGGTTTGGC AATTCTCTAC CAATGAACCA
    ATCTTTTCAT CCCCATGTAC CTCAGCATCA GAGCAAGAAA TATTTTTTGG TTCTCATGAC
    TGCTTTATCT ACTGTTGTAG TATGAAAGGT CACCTCCAGT GGAAATTTGA AACTACTTCA
    AGAGTGTATT CAACGCCTTT TGTTTTCCGT AACCACAACC ATAACAGTGA AATGTTACTG
    GCAGCGGCAT CTACTGATGG GAAACTGTGG ATCTTGGAAT CTAAGAGTGG ACAGTTGCAA
    AGTGTGTATG AACTTCCTGG AGAAGTTTTC TCTTCTCCTG TGGTCTGGGA ATCAATGCTT
    ATTATTGGAT GTAGAAATAA TTATGTTTAT TGTCTGGATG TATTGGGTGA CAAAGGTAAT
    GAAGTGCAGT CCTTAGAATA TACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849348.1
    CDS146..2632
    Translation

    Target ORF information:

    RefSeq Version XM_024993580.1
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993580.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTATAGTG CCAAACATCC AGCATTTTCG GCAACGCCAA CATTGCTTAG AAGATTTGGA 
    TCTCAGCTCA TCAAGTCAAC TGTTTTATCC GCCAGTACCT CTCTTCGCAT ATTGGCCCTT
    GGTGGTGAAG CATTTCCATC ATTAACTGTT CTCAAAAGCT GGAGAGGAGT GGGCAATAAA
    ACACAAATAT TTAATATTTA TGGCATCACA GAGGTATCAA GTTGGGCGAC TTTCTACAGG
    ATTCCAGAGA AGATTCTTAA CTCTACTTTG AAATGTGAAT TGCCTGTACA ACTGGGATTC
    CCACTGCTTG GAACAGTAGT TGAAGTCAGA GATACTAACG GTTTCACAAT TCAAGAAGGC
    AATGGCCAAG TATTTTTAGG TGGCAGAAAT AGAGTATGTT TTCTCGATGG CGAAGTGACG
    GTACCACTTG GCACAATGAG AGCCACAGGA GACTTTGTGA CTATAAAAGA TGGAGAGATA
    TTTTTCTTGG GACGAAAGGA CAGTCAGATC AAACGTCATG GCAAACGTCT TAACATAGAA
    CTTGTACAAC AGATTGCTGA AGGTCTTCAA CAAGTGGAGT CTTGTGCAGT TACATGGTAT
    AACCAGGAAA AATTGATTTT GTTCATGGTG ACTAAAAATG ATTTAGTAAA GGATCACATC
    TTTGAAGAAC TGCAGAAACA TCTTCCAAGT CATGCCATCC CAGATGAGCT TGTGTTAATT
    AATTCTCTGC CATTTACATC TCATGGCAAA ATTGATGTTT CTGAGTTAAA TAAGATATAT
    TTAAACTACA GAGACTTGAA GTCTGAGTGT AAGCTCAGTG GAAAAGAGGA ACTTTGGGAA
    AAATTACACC ATTTGTGGAA GTCTGTTCTG AGTCTCTCAG AAGATGCTTT GAAGATTCCT
    GATGAGTCAC TCTTCTTAAA TAGTGGTGGA GATTCCTTGA AATCCATACG GCTTCTCAAT
    GAGATTGAAA ACCTTGTTGG CACATCAGTA CCTGGGCTTC TGGAAATTAT TCTCAGCAGT
    TCTATTTTAC GGATTTACAA TCACATCCTT CAAACAGTGT TTTCAGATAA AGATATGACA
    CTTAGCAAGA ATCATGCCAT GAAAAGAAAA TTCAACGTTA CTCAAGAGGA AACCAGTGGA
    AAATCTTTAC ATCAGGAAAC TGTCATGCCT TTAAATTGTG ACAAGGAGAT CAATGCTTTT
    ATTGCACTGA GCAGAGGGAA TCAGATTTTG TCTTTGAATA CTGATAAGTA TTTAACTAAG
    TTGGGACATT GCCCTTCAAC CTATTCTTCT GATTTAAATT CACAGACTGA CATTCAAAAT
    AGAGAAAGCC TAAATACTCC ACCTCTTATT GGGAAGTCAG AAGATCCATC CTGTGTTGTG
    GAAGTTTCTG AAGAGGAAAC ACCTGTGATT AGGGCTGAGA AAATGGAGTT TCGTGTGAGG
    TGGAGGTCAG ACACAGGCAA ATGTGTGGAT GCTTCACCTC TGGTTGTAAT ACCAGCTATT
    GATAAGTCAT CTGCAACTGT GTACATTGGC TCTCATTCTC ATAGAATGAT AGCAGTTGAT
    CTTTACTCTG GAAAGGTGAA ATGGGAACAG ATTTTAGGAG ATCGCATTGA ATCCTCAGCA
    TGTGTATCTA AGTGTGGAAA CTTTATTGTA GTGGGCTGTT ATAATGGGTT AGTTTATGTT
    CTGAAAAGTA ATAGTGGAGA AAAATACTGG ATGTTTAGTA CTGAGGATGC TGTCAAAAGC
    TCAGCAACCA TGGATCCAAC CACAGGACTT CTTTACATTG GATCTCATGA CCAGCATGCA
    TACGCTTTGG ATATATATAA GAAGAAGTGT GTTTGGAAGT TGAAATGTGG AGGAACTGTT
    TTTTCTTCTC CTTGTTTGAG CCTGATTCCA TCTCACTTGT ATTTTGCTAC ACTGGGAGGA
    CTTTTACTGG CTATAAATCC TGCCACTGGG GACAGACTTT GGAAACATTC CTGTGGAAAG
    CCACTCTTTT CTTCTCCACG GTGTTGCCTA CAGTATGTTT TTATTGGCTG TGTAGATGGA
    AATTTACTCT GCTTTACTCA CTTTGGAGAA CAGGTTTGGC AATTCTCTAC CAATGAACCA
    ATCTTTTCAT CCCCATGTAC CTCAGCATCA GAGCAAGAAA TATTTTTTGG TTCTCATGAC
    TGCTTTATCT ACTGTTGTAG TATGAAAGGT CACCTCCAGT GGAAATTTGA AACTACTTCA
    AGAGTGTATT CAACGCCTTT TGTTTTCCGT AACCACAACC ATAACAGTGA AATGTTACTG
    GCAGCGGCAT CTACTGATGG GAAACTGTGG ATCTTGGAAT CTAAGAGTGG ACAGTTGCAA
    AGTGTGTATG AACTTCCTGG AGAAGTTTTC TCTTCTCCTG TGGTCTGGGA ATCAATGCTT
    ATTATTGGAT GTAGAAATAA TTATGTTTAT TGTCTGGATG TATTGGGTGA CAAAGGTAAT
    GAAGTGCAGT CCTTAGAATA TACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235570
    Clone ID Related Accession (Same CDS sequence) XM_010806148.3
    Accession Version XM_010806148.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2052bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product beta-alanine-activating enzyme isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806148.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAGAGCCA CAGGAGACTT TGTGACTATA AAAGATGGAG AGATATTTTT CTTGGGACGA 
    AAGGACAGTC AGATCAAACG TCATGGCAAA CGTCTTAACA TAGAACTTGT ACAACAGATT
    GCTGAAGGTC TTCAACAAGT GGAGTCTTGT GCAGTTACAT GGTATAACCA GGAAAAATTG
    ATTTTGTTCA TGGTGACTAA AAATGATTTA GTAAAGGATC ACATCTTTGA AGAACTGCAG
    AAACATCTTC CAAGTCATGC CATCCCAGAT GAGCTTGTGT TAATTAATTC TCTGCCATTT
    ACATCTCATG GCAAAATTGA TGTTTCTGAG TTAAATAAGA TATATTTAAA CTACAGAGAC
    TTGAAGTCTG AGTGTAAGCT CAGTGGAAAA GAGGAACTTT GGGAAAAATT ACACCATTTG
    TGGAAGTCTG TTCTGAGTCT CTCAGAAGAT GCTTTGAAGA TTCCTGATGA GTCACTCTTC
    TTAAATAGTG GTGGAGATTC CTTGAAATCC ATACGGCTTC TCAATGAGAT TGAAAACCTT
    GTTGGCACAT CAGTACCTGG GCTTCTGGAA ATTATTCTCA GCAGTTCTAT TTTACGGATT
    TACAATCACA TCCTTCAAAC AGTGTTTTCA GATAAAGATA TGACACTTAG CAAGAATCAT
    GCCATGAAAA GAAAATTCAA CGTTACTCAA GAGGAAACCA GTGGAAAATC TTTACATCAG
    GAAACTGTCA TGCCTTTAAA TTGTGACAAG GAGATCAATG CTTTTATTGC ACTGAGCAGA
    GGGAATCAGA TTTTGTCTTT GAATACTGAT AAGTATTTAA CTAAGTTGGG ACATTGCCCT
    TCAACCTATT CTTCTGATTT AAATTCACAG ACTGACATTC AAAATAGAGA AAGCCTAAAT
    ACTCCACCTC TTATTGGGAA GTCAGAAGAT CCATCCTGTG TTGTGGAAGT TTCTGAAGAG
    GAAACACCTG TGATTAGGGC TGAGAAAATG GAGTTTCGTG TGAGGTGGAG GTCAGACACA
    GGCAAATGTG TGGATGCTTC ACCTCTGGTT GTAATACCAG CTATTGATAA GTCATCTGCA
    ACTGTGTACA TTGGCTCTCA TTCTCATAGA ATGATAGCAG TTGATCTTTA CTCTGGAAAG
    GTGAAATGGG AACAGATTTT AGGAGATCGC ATTGAATCCT CAGCATGTGT ATCTAAGTGT
    GGAAACTTTA TTGTAGTGGG CTGTTATAAT GGGTTAGTTT ATGTTCTGAA AAGTAATAGT
    GGAGAAAAAT ACTGGATGTT TAGTACTGAG GATGCTGTCA AAAGCTCAGC AACCATGGAT
    CCAACCACAG GACTTCTTTA CATTGGATCT CATGACCAGC ATGCATACGC TTTGGATATA
    TATAAGAAGA AGTGTGTTTG GAAGTTGAAA TGTGGAGGAA CTGTTTTTTC TTCTCCTTGT
    TTGAGCCTGA TTCCATCTCA CTTGTATTTT GCTACACTGG GAGGACTTTT ACTGGCTATA
    AATCCTGCCA CTGGGGACAG ACTTTGGAAA CATTCCTGTG GAAAGCCACT CTTTTCTTCT
    CCACGGTGTT GCCTACAGTA TGTTTTTATT GGCTGTGTAG ATGGAAATTT ACTCTGCTTT
    ACTCACTTTG GAGAACAGGT TTGGCAATTC TCTACCAATG AACCAATCTT TTCATCCCCA
    TGTACCTCAG CATCAGAGCA AGAAATATTT TTTGGTTCTC ATGACTGCTT TATCTACTGT
    TGTAGTATGA AAGGTCACCT CCAGTGGAAA TTTGAAACTA CTTCAAGAGT GTATTCAACG
    CCTTTTGTTT TCCGTAACCA CAACCATAAC AGTGAAATGT TACTGGCAGC GGCATCTACT
    GATGGGAAAC TGTGGATCTT GGAATCTAAG AGTGGACAGT TGCAAAGTGT GTATGAACTT
    CCTGGAGAAG TTTTCTCTTC TCCTGTGGTC TGGGAATCAA TGCTTATTAT TGGATGTAGA
    AATAATTATG TTTATTGTCT GGATGTATTG GGTGACAAAG GTAATGAAGT GCAGTCCTTA
    GAATATACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804450.2
    CDS188..2239
    Translation

    Target ORF information:

    RefSeq Version XM_010806148.3
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806148.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAGAGCCA CAGGAGACTT TGTGACTATA AAAGATGGAG AGATATTTTT CTTGGGACGA 
    AAGGACAGTC AGATCAAACG TCATGGCAAA CGTCTTAACA TAGAACTTGT ACAACAGATT
    GCTGAAGGTC TTCAACAAGT GGAGTCTTGT GCAGTTACAT GGTATAACCA GGAAAAATTG
    ATTTTGTTCA TGGTGACTAA AAATGATTTA GTAAAGGATC ACATCTTTGA AGAACTGCAG
    AAACATCTTC CAAGTCATGC CATCCCAGAT GAGCTTGTGT TAATTAATTC TCTGCCATTT
    ACATCTCATG GCAAAATTGA TGTTTCTGAG TTAAATAAGA TATATTTAAA CTACAGAGAC
    TTGAAGTCTG AGTGTAAGCT CAGTGGAAAA GAGGAACTTT GGGAAAAATT ACACCATTTG
    TGGAAGTCTG TTCTGAGTCT CTCAGAAGAT GCTTTGAAGA TTCCTGATGA GTCACTCTTC
    TTAAATAGTG GTGGAGATTC CTTGAAATCC ATACGGCTTC TCAATGAGAT TGAAAACCTT
    GTTGGCACAT CAGTACCTGG GCTTCTGGAA ATTATTCTCA GCAGTTCTAT TTTACGGATT
    TACAATCACA TCCTTCAAAC AGTGTTTTCA GATAAAGATA TGACACTTAG CAAGAATCAT
    GCCATGAAAA GAAAATTCAA CGTTACTCAA GAGGAAACCA GTGGAAAATC TTTACATCAG
    GAAACTGTCA TGCCTTTAAA TTGTGACAAG GAGATCAATG CTTTTATTGC ACTGAGCAGA
    GGGAATCAGA TTTTGTCTTT GAATACTGAT AAGTATTTAA CTAAGTTGGG ACATTGCCCT
    TCAACCTATT CTTCTGATTT AAATTCACAG ACTGACATTC AAAATAGAGA AAGCCTAAAT
    ACTCCACCTC TTATTGGGAA GTCAGAAGAT CCATCCTGTG TTGTGGAAGT TTCTGAAGAG
    GAAACACCTG TGATTAGGGC TGAGAAAATG GAGTTTCGTG TGAGGTGGAG GTCAGACACA
    GGCAAATGTG TGGATGCTTC ACCTCTGGTT GTAATACCAG CTATTGATAA GTCATCTGCA
    ACTGTGTACA TTGGCTCTCA TTCTCATAGA ATGATAGCAG TTGATCTTTA CTCTGGAAAG
    GTGAAATGGG AACAGATTTT AGGAGATCGC ATTGAATCCT CAGCATGTGT ATCTAAGTGT
    GGAAACTTTA TTGTAGTGGG CTGTTATAAT GGGTTAGTTT ATGTTCTGAA AAGTAATAGT
    GGAGAAAAAT ACTGGATGTT TAGTACTGAG GATGCTGTCA AAAGCTCAGC AACCATGGAT
    CCAACCACAG GACTTCTTTA CATTGGATCT CATGACCAGC ATGCATACGC TTTGGATATA
    TATAAGAAGA AGTGTGTTTG GAAGTTGAAA TGTGGAGGAA CTGTTTTTTC TTCTCCTTGT
    TTGAGCCTGA TTCCATCTCA CTTGTATTTT GCTACACTGG GAGGACTTTT ACTGGCTATA
    AATCCTGCCA CTGGGGACAG ACTTTGGAAA CATTCCTGTG GAAAGCCACT CTTTTCTTCT
    CCACGGTGTT GCCTACAGTA TGTTTTTATT GGCTGTGTAG ATGGAAATTT ACTCTGCTTT
    ACTCACTTTG GAGAACAGGT TTGGCAATTC TCTACCAATG AACCAATCTT TTCATCCCCA
    TGTACCTCAG CATCAGAGCA AGAAATATTT TTTGGTTCTC ATGACTGCTT TATCTACTGT
    TGTAGTATGA AAGGTCACCT CCAGTGGAAA TTTGAAACTA CTTCAAGAGT GTATTCAACG
    CCTTTTGTTT TCCGTAACCA CAACCATAAC AGTGAAATGT TACTGGCAGC GGCATCTACT
    GATGGGAAAC TGTGGATCTT GGAATCTAAG AGTGGACAGT TGCAAAGTGT GTATGAACTT
    CCTGGAGAAG TTTTCTCTTC TCCTGTGGTC TGGGAATCAA TGCTTATTAT TGGATGTAGA
    AATAATTATG TTTATTGTCT GGATGTATTG GGTGACAAAG GTAATGAAGT GCAGTCCTTA
    GAATATACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90092
    Clone ID Related Accession (Same CDS sequence) XM_015471629.1 , XM_015463766.1
    Accession Version XM_015471629.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2922bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product acyl-CoA synthetase family member 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103975.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTG CAACGCCAAC ATTGCTTAGA AGATTTGGAT CTCAGCTCAT CAAGTCAACT
    GTTTTATCCG CCAGTACCTC TCTTCGCATA TTGGCCCTTG GTGGTGAAGC ATTTCCATCA
    TTAACTGTTC TCAAAAGCTG GAGAGGAGTG GGCAATAAAA CACAAATATT TAATATTTAT
    GGCATCACAG AGGTATCAAG TTGGGCGACT TTCTACAGGA TTCCAGAGAA GATTCTTAAC
    TCTACTTTGA AGTACGGGTT TCTGTTACTA CGAAGAGTAG AGAGAGGATG TGAATTGCCT
    GTACAACTGG GATTCCCACT GCTTGGAACA GTAGTTGAAG TCAGAGATAC TAACGGTTTC
    ACAATTCAAG AAGGCAATGG CCAAGTATTT TTAGGTGGCA GAAATAGAGT ATGTTTTCTC
    GATGGCGAAG TGACGGTACC ACTTGGCACA ATGAGAGCCA CAGGAGACTT TGTGACTATA
    AAAGATGGAG AGATATTTTT CTTGGGACGA AAGGACAGTC AGATCAAACG TCATGGCAAA
    CGTCTTAACA TAGAACTTGT ACAACAGATT GCTGAAGGTC TTCAACAAGT GGAGTCTTGT
    GCAGTTACAT GGTATAACCA GGAAAAATTG ATTTTGTTCA TGGTGACTAA AAATGATTTA
    GTAAAGGATC ACATCTTTGA AGAACTGCAG AAACATCTTC CAAGTCATGC CATCCCAGAT
    GAGCTTGTGT TAATTAATTC TCTGCCATTT ACATCTCATG GCAAAATTGA TGTTTCTGAG
    TTAAATAAGA TATATTTAAA CTACAGAGAC TTGAAGTCTG AGTGTAAGCT CAGTGGAAAA
    GAGGAACTTT GGGAAAAATT ACACCATTTG TGGAAGTCTG TTCTGAGTCT CTCAGAAGAT
    GCTTTGAAGA TTCCTGATGA GTCACTCTTC TTAAATAGTG GTGGAGATTC CTTGAAATCC
    ATACGGCTTC TCAATGAGAT TGAAAACCTT GTTGGCACAT CAGTACCTGG GCTTCTGGAA
    ATTATTCTCA GCAGTTCTAT TTTACGGATT TACAATCACA TCCTTCAAAC AGTGTTTTCA
    GATAAAGATA TGACACTTAG CAAGAATCAT GCCATGAAAA GAAAATTCAA CGTTACTCAA
    GAGGAAACCA GTGGAAAATC TTTACATCAG GAAACTGTCA TGCCTTTAAA TTGTGACAAG
    GAGATCGATG CTTTTATTGC ACTGAGCAGA GGGAATCAGA TTTTGTCTTT GAATACTGAT
    AAGTATTTAA CTAAGTTGGG ACATTGCCCT TCAACCTATT CTTCTGATTT AAATTCACAG
    ACTGACATTC AAAATAGAGA AAGCCTAAAT ACTCCACCTC TTATTGGGAA GTCAGAAGAT
    CCATCCTGTG TTGTGGAAGT TTCTGAAGAG GAAACACCTG TGATTAGGGC TGAGAAAATG
    GAGTTTCGTG TGAGGTGGAG GTCAGACACA GGCAAATGTG TGGATGCTTC ACCTCTGGTT
    GTAATACCAG CTATTGATAA GTCATCTGCA ACTGTGTACA TTGGCTCTCA TTCTCATAGA
    ATGATAGCAG TTGATCTTTA CTCTGGAAAG GTGAAATGGG AACAGATTTT AGGAGATCGC
    ATTGAATCCT CAGCATGTGT ATCTAAGTGT GGAAACTTTA TTGTAGTGGG CTGTTATAAT
    GGGTTAGTTT ATGTTCTGAA AAGTAATAGT GGAGAAAAAT ACTGGATGTT TAGTACTGAG
    GATGCTGTCA AAAGCTCAGC AACCATGGAT CCAACCACAG GACTTCTTTA CATTGGATCT
    CATGACCAGC ATGCATACGC TTTGGATATA TATAAGAAGA AGTGTGTTTG GAAATTGAAA
    TGTGGAGGAA CTGTTTTTTC TTCTCCTTGT TTGAGCCTGA TTCCATCTCA CTTGTATTTT
    GCTACACTGG GAGGACTTTT ACTGGCTATA AATCCTGCCA CTGGGGACAG ACTTTGGAAA
    CATTCCTGTG GAAAGCCACT CTTTTCTTCT CCACGGTGTT GCCTACAGTA TGTTTTTATT
    GGCTGTGTAG ATGGAAATTT ACTCTGCTTT ACTCACTTTG GAGAACAGGT TTGGCAATTC
    TCTACCAATG AACCAATCTT TTCATCCCCA TGTACCTCAG CATCAGAGCA AGAAATATTT
    TTTGGTTCTC ATGACTGCTT TATCTACTGT TGTAGTATGA AAGGTCACCT CCAGTGGAAA
    TTTGAAACTA CTTCAAGAGT GTATTCAACG CCTTTTGTTT TCCGTAACCA CAACCATAAC
    AGTGAAATGT TACTGGCAGC GGCATCTACT GATGGGAAAC TGTGGATCTT GGAATCTAAG
    AGTGGACAGT TGCAAAGTGT GTATGAACTT CCTGGAGAAG TTTTCTCTTC TCCTGTGGTC
    TGGGAATCAA TGCTTATTAT TGGATGTAGA AATAATTATG TTTATTGTCT GGATGTATTG
    GGTGACAAAG GTAATGAAGT GCAGTCCTTA GAATATACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327115.1
    CDS437..3358
    Translation

    Target ORF information:

    RefSeq Version XM_015471629.1
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471629.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTG CAACGCCAAC ATTGCTTAGA AGATTTGGAT CTCAGCTCAT CAAGTCAACT
    GTTTTATCCG CCAGTACCTC TCTTCGCATA TTGGCCCTTG GTGGTGAAGC ATTTCCATCA
    TTAACTGTTC TCAAAAGCTG GAGAGGAGTG GGCAATAAAA CACAAATATT TAATATTTAT
    GGCATCACAG AGGTATCAAG TTGGGCGACT TTCTACAGGA TTCCAGAGAA GATTCTTAAC
    TCTACTTTGA AGTACGGGTT TCTGTTACTA CGAAGAGTAG AGAGAGGATG TGAATTGCCT
    GTACAACTGG GATTCCCACT GCTTGGAACA GTAGTTGAAG TCAGAGATAC TAACGGTTTC
    ACAATTCAAG AAGGCAATGG CCAAGTATTT TTAGGTGGCA GAAATAGAGT ATGTTTTCTC
    GATGGCGAAG TGACGGTACC ACTTGGCACA ATGAGAGCCA CAGGAGACTT TGTGACTATA
    AAAGATGGAG AGATATTTTT CTTGGGACGA AAGGACAGTC AGATCAAACG TCATGGCAAA
    CGTCTTAACA TAGAACTTGT ACAACAGATT GCTGAAGGTC TTCAACAAGT GGAGTCTTGT
    GCAGTTACAT GGTATAACCA GGAAAAATTG ATTTTGTTCA TGGTGACTAA AAATGATTTA
    GTAAAGGATC ACATCTTTGA AGAACTGCAG AAACATCTTC CAAGTCATGC CATCCCAGAT
    GAGCTTGTGT TAATTAATTC TCTGCCATTT ACATCTCATG GCAAAATTGA TGTTTCTGAG
    TTAAATAAGA TATATTTAAA CTACAGAGAC TTGAAGTCTG AGTGTAAGCT CAGTGGAAAA
    GAGGAACTTT GGGAAAAATT ACACCATTTG TGGAAGTCTG TTCTGAGTCT CTCAGAAGAT
    GCTTTGAAGA TTCCTGATGA GTCACTCTTC TTAAATAGTG GTGGAGATTC CTTGAAATCC
    ATACGGCTTC TCAATGAGAT TGAAAACCTT GTTGGCACAT CAGTACCTGG GCTTCTGGAA
    ATTATTCTCA GCAGTTCTAT TTTACGGATT TACAATCACA TCCTTCAAAC AGTGTTTTCA
    GATAAAGATA TGACACTTAG CAAGAATCAT GCCATGAAAA GAAAATTCAA CGTTACTCAA
    GAGGAAACCA GTGGAAAATC TTTACATCAG GAAACTGTCA TGCCTTTAAA TTGTGACAAG
    GAGATCGATG CTTTTATTGC ACTGAGCAGA GGGAATCAGA TTTTGTCTTT GAATACTGAT
    AAGTATTTAA CTAAGTTGGG ACATTGCCCT TCAACCTATT CTTCTGATTT AAATTCACAG
    ACTGACATTC AAAATAGAGA AAGCCTAAAT ACTCCACCTC TTATTGGGAA GTCAGAAGAT
    CCATCCTGTG TTGTGGAAGT TTCTGAAGAG GAAACACCTG TGATTAGGGC TGAGAAAATG
    GAGTTTCGTG TGAGGTGGAG GTCAGACACA GGCAAATGTG TGGATGCTTC ACCTCTGGTT
    GTAATACCAG CTATTGATAA GTCATCTGCA ACTGTGTACA TTGGCTCTCA TTCTCATAGA
    ATGATAGCAG TTGATCTTTA CTCTGGAAAG GTGAAATGGG AACAGATTTT AGGAGATCGC
    ATTGAATCCT CAGCATGTGT ATCTAAGTGT GGAAACTTTA TTGTAGTGGG CTGTTATAAT
    GGGTTAGTTT ATGTTCTGAA AAGTAATAGT GGAGAAAAAT ACTGGATGTT TAGTACTGAG
    GATGCTGTCA AAAGCTCAGC AACCATGGAT CCAACCACAG GACTTCTTTA CATTGGATCT
    CATGACCAGC ATGCATACGC TTTGGATATA TATAAGAAGA AGTGTGTTTG GAAATTGAAA
    TGTGGAGGAA CTGTTTTTTC TTCTCCTTGT TTGAGCCTGA TTCCATCTCA CTTGTATTTT
    GCTACACTGG GAGGACTTTT ACTGGCTATA AATCCTGCCA CTGGGGACAG ACTTTGGAAA
    CATTCCTGTG GAAAGCCACT CTTTTCTTCT CCACGGTGTT GCCTACAGTA TGTTTTTATT
    GGCTGTGTAG ATGGAAATTT ACTCTGCTTT ACTCACTTTG GAGAACAGGT TTGGCAATTC
    TCTACCAATG AACCAATCTT TTCATCCCCA TGTACCTCAG CATCAGAGCA AGAAATATTT
    TTTGGTTCTC ATGACTGCTT TATCTACTGT TGTAGTATGA AAGGTCACCT CCAGTGGAAA
    TTTGAAACTA CTTCAAGAGT GTATTCAACG CCTTTTGTTT TCCGTAACCA CAACCATAAC
    AGTGAAATGT TACTGGCAGC GGCATCTACT GATGGGAAAC TGTGGATCTT GGAATCTAAG
    AGTGGACAGT TGCAAAGTGT GTATGAACTT CCTGGAGAAG TTTTCTCTTC TCCTGTGGTC
    TGGGAATCAA TGCTTATTAT TGGATGTAGA AATAATTATG TTTATTGTCT GGATGTATTG
    GGTGACAAAG GTAATGAAGT GCAGTCCTTA GAATATACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90092
    Clone ID Related Accession (Same CDS sequence) XM_015471629.1 , XM_015463766.1
    Accession Version XM_015463766.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2922bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product acyl-CoA synthetase family member 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTG CAACGCCAAC ATTGCTTAGA AGATTTGGAT CTCAGCTCAT CAAGTCAACT
    GTTTTATCCG CCAGTACCTC TCTTCGCATA TTGGCCCTTG GTGGTGAAGC ATTTCCATCA
    TTAACTGTTC TCAAAAGCTG GAGAGGAGTG GGCAATAAAA CACAAATATT TAATATTTAT
    GGCATCACAG AGGTATCAAG TTGGGCGACT TTCTACAGGA TTCCAGAGAA GATTCTTAAC
    TCTACTTTGA AGTACGGGTT TCTGTTACTA CGAAGAGTAG AGAGAGGATG TGAATTGCCT
    GTACAACTGG GATTCCCACT GCTTGGAACA GTAGTTGAAG TCAGAGATAC TAACGGTTTC
    ACAATTCAAG AAGGCAATGG CCAAGTATTT TTAGGTGGCA GAAATAGAGT ATGTTTTCTC
    GATGGCGAAG TGACGGTACC ACTTGGCACA ATGAGAGCCA CAGGAGACTT TGTGACTATA
    AAAGATGGAG AGATATTTTT CTTGGGACGA AAGGACAGTC AGATCAAACG TCATGGCAAA
    CGTCTTAACA TAGAACTTGT ACAACAGATT GCTGAAGGTC TTCAACAAGT GGAGTCTTGT
    GCAGTTACAT GGTATAACCA GGAAAAATTG ATTTTGTTCA TGGTGACTAA AAATGATTTA
    GTAAAGGATC ACATCTTTGA AGAACTGCAG AAACATCTTC CAAGTCATGC CATCCCAGAT
    GAGCTTGTGT TAATTAATTC TCTGCCATTT ACATCTCATG GCAAAATTGA TGTTTCTGAG
    TTAAATAAGA TATATTTAAA CTACAGAGAC TTGAAGTCTG AGTGTAAGCT CAGTGGAAAA
    GAGGAACTTT GGGAAAAATT ACACCATTTG TGGAAGTCTG TTCTGAGTCT CTCAGAAGAT
    GCTTTGAAGA TTCCTGATGA GTCACTCTTC TTAAATAGTG GTGGAGATTC CTTGAAATCC
    ATACGGCTTC TCAATGAGAT TGAAAACCTT GTTGGCACAT CAGTACCTGG GCTTCTGGAA
    ATTATTCTCA GCAGTTCTAT TTTACGGATT TACAATCACA TCCTTCAAAC AGTGTTTTCA
    GATAAAGATA TGACACTTAG CAAGAATCAT GCCATGAAAA GAAAATTCAA CGTTACTCAA
    GAGGAAACCA GTGGAAAATC TTTACATCAG GAAACTGTCA TGCCTTTAAA TTGTGACAAG
    GAGATCGATG CTTTTATTGC ACTGAGCAGA GGGAATCAGA TTTTGTCTTT GAATACTGAT
    AAGTATTTAA CTAAGTTGGG ACATTGCCCT TCAACCTATT CTTCTGATTT AAATTCACAG
    ACTGACATTC AAAATAGAGA AAGCCTAAAT ACTCCACCTC TTATTGGGAA GTCAGAAGAT
    CCATCCTGTG TTGTGGAAGT TTCTGAAGAG GAAACACCTG TGATTAGGGC TGAGAAAATG
    GAGTTTCGTG TGAGGTGGAG GTCAGACACA GGCAAATGTG TGGATGCTTC ACCTCTGGTT
    GTAATACCAG CTATTGATAA GTCATCTGCA ACTGTGTACA TTGGCTCTCA TTCTCATAGA
    ATGATAGCAG TTGATCTTTA CTCTGGAAAG GTGAAATGGG AACAGATTTT AGGAGATCGC
    ATTGAATCCT CAGCATGTGT ATCTAAGTGT GGAAACTTTA TTGTAGTGGG CTGTTATAAT
    GGGTTAGTTT ATGTTCTGAA AAGTAATAGT GGAGAAAAAT ACTGGATGTT TAGTACTGAG
    GATGCTGTCA AAAGCTCAGC AACCATGGAT CCAACCACAG GACTTCTTTA CATTGGATCT
    CATGACCAGC ATGCATACGC TTTGGATATA TATAAGAAGA AGTGTGTTTG GAAATTGAAA
    TGTGGAGGAA CTGTTTTTTC TTCTCCTTGT TTGAGCCTGA TTCCATCTCA CTTGTATTTT
    GCTACACTGG GAGGACTTTT ACTGGCTATA AATCCTGCCA CTGGGGACAG ACTTTGGAAA
    CATTCCTGTG GAAAGCCACT CTTTTCTTCT CCACGGTGTT GCCTACAGTA TGTTTTTATT
    GGCTGTGTAG ATGGAAATTT ACTCTGCTTT ACTCACTTTG GAGAACAGGT TTGGCAATTC
    TCTACCAATG AACCAATCTT TTCATCCCCA TGTACCTCAG CATCAGAGCA AGAAATATTT
    TTTGGTTCTC ATGACTGCTT TATCTACTGT TGTAGTATGA AAGGTCACCT CCAGTGGAAA
    TTTGAAACTA CTTCAAGAGT GTATTCAACG CCTTTTGTTT TCCGTAACCA CAACCATAAC
    AGTGAAATGT TACTGGCAGC GGCATCTACT GATGGGAAAC TGTGGATCTT GGAATCTAAG
    AGTGGACAGT TGCAAAGTGT GTATGAACTT CCTGGAGAAG TTTTCTCTTC TCCTGTGGTC
    TGGGAATCAA TGCTTATTAT TGGATGTAGA AATAATTATG TTTATTGTCT GGATGTATTG
    GGTGACAAAG GTAATGAAGT GCAGTCCTTA GAATATACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319252.1
    CDS437..3358
    Translation

    Target ORF information:

    RefSeq Version XM_015463766.1
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463766.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTG CAACGCCAAC ATTGCTTAGA AGATTTGGAT CTCAGCTCAT CAAGTCAACT
    GTTTTATCCG CCAGTACCTC TCTTCGCATA TTGGCCCTTG GTGGTGAAGC ATTTCCATCA
    TTAACTGTTC TCAAAAGCTG GAGAGGAGTG GGCAATAAAA CACAAATATT TAATATTTAT
    GGCATCACAG AGGTATCAAG TTGGGCGACT TTCTACAGGA TTCCAGAGAA GATTCTTAAC
    TCTACTTTGA AGTACGGGTT TCTGTTACTA CGAAGAGTAG AGAGAGGATG TGAATTGCCT
    GTACAACTGG GATTCCCACT GCTTGGAACA GTAGTTGAAG TCAGAGATAC TAACGGTTTC
    ACAATTCAAG AAGGCAATGG CCAAGTATTT TTAGGTGGCA GAAATAGAGT ATGTTTTCTC
    GATGGCGAAG TGACGGTACC ACTTGGCACA ATGAGAGCCA CAGGAGACTT TGTGACTATA
    AAAGATGGAG AGATATTTTT CTTGGGACGA AAGGACAGTC AGATCAAACG TCATGGCAAA
    CGTCTTAACA TAGAACTTGT ACAACAGATT GCTGAAGGTC TTCAACAAGT GGAGTCTTGT
    GCAGTTACAT GGTATAACCA GGAAAAATTG ATTTTGTTCA TGGTGACTAA AAATGATTTA
    GTAAAGGATC ACATCTTTGA AGAACTGCAG AAACATCTTC CAAGTCATGC CATCCCAGAT
    GAGCTTGTGT TAATTAATTC TCTGCCATTT ACATCTCATG GCAAAATTGA TGTTTCTGAG
    TTAAATAAGA TATATTTAAA CTACAGAGAC TTGAAGTCTG AGTGTAAGCT CAGTGGAAAA
    GAGGAACTTT GGGAAAAATT ACACCATTTG TGGAAGTCTG TTCTGAGTCT CTCAGAAGAT
    GCTTTGAAGA TTCCTGATGA GTCACTCTTC TTAAATAGTG GTGGAGATTC CTTGAAATCC
    ATACGGCTTC TCAATGAGAT TGAAAACCTT GTTGGCACAT CAGTACCTGG GCTTCTGGAA
    ATTATTCTCA GCAGTTCTAT TTTACGGATT TACAATCACA TCCTTCAAAC AGTGTTTTCA
    GATAAAGATA TGACACTTAG CAAGAATCAT GCCATGAAAA GAAAATTCAA CGTTACTCAA
    GAGGAAACCA GTGGAAAATC TTTACATCAG GAAACTGTCA TGCCTTTAAA TTGTGACAAG
    GAGATCGATG CTTTTATTGC ACTGAGCAGA GGGAATCAGA TTTTGTCTTT GAATACTGAT
    AAGTATTTAA CTAAGTTGGG ACATTGCCCT TCAACCTATT CTTCTGATTT AAATTCACAG
    ACTGACATTC AAAATAGAGA AAGCCTAAAT ACTCCACCTC TTATTGGGAA GTCAGAAGAT
    CCATCCTGTG TTGTGGAAGT TTCTGAAGAG GAAACACCTG TGATTAGGGC TGAGAAAATG
    GAGTTTCGTG TGAGGTGGAG GTCAGACACA GGCAAATGTG TGGATGCTTC ACCTCTGGTT
    GTAATACCAG CTATTGATAA GTCATCTGCA ACTGTGTACA TTGGCTCTCA TTCTCATAGA
    ATGATAGCAG TTGATCTTTA CTCTGGAAAG GTGAAATGGG AACAGATTTT AGGAGATCGC
    ATTGAATCCT CAGCATGTGT ATCTAAGTGT GGAAACTTTA TTGTAGTGGG CTGTTATAAT
    GGGTTAGTTT ATGTTCTGAA AAGTAATAGT GGAGAAAAAT ACTGGATGTT TAGTACTGAG
    GATGCTGTCA AAAGCTCAGC AACCATGGAT CCAACCACAG GACTTCTTTA CATTGGATCT
    CATGACCAGC ATGCATACGC TTTGGATATA TATAAGAAGA AGTGTGTTTG GAAATTGAAA
    TGTGGAGGAA CTGTTTTTTC TTCTCCTTGT TTGAGCCTGA TTCCATCTCA CTTGTATTTT
    GCTACACTGG GAGGACTTTT ACTGGCTATA AATCCTGCCA CTGGGGACAG ACTTTGGAAA
    CATTCCTGTG GAAAGCCACT CTTTTCTTCT CCACGGTGTT GCCTACAGTA TGTTTTTATT
    GGCTGTGTAG ATGGAAATTT ACTCTGCTTT ACTCACTTTG GAGAACAGGT TTGGCAATTC
    TCTACCAATG AACCAATCTT TTCATCCCCA TGTACCTCAG CATCAGAGCA AGAAATATTT
    TTTGGTTCTC ATGACTGCTT TATCTACTGT TGTAGTATGA AAGGTCACCT CCAGTGGAAA
    TTTGAAACTA CTTCAAGAGT GTATTCAACG CCTTTTGTTT TCCGTAACCA CAACCATAAC
    AGTGAAATGT TACTGGCAGC GGCATCTACT GATGGGAAAC TGTGGATCTT GGAATCTAAG
    AGTGGACAGT TGCAAAGTGT GTATGAACTT CCTGGAGAAG TTTTCTCTTC TCCTGTGGTC
    TGGGAATCAA TGCTTATTAT TGGATGTAGA AATAATTATG TTTATTGTCT GGATGTATTG
    GGTGACAAAG GTAATGAAGT GCAGTCCTTA GAATATACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235563
    Clone ID Related Accession (Same CDS sequence) XM_002688264.6
    Accession Version XM_002688264.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3399bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product beta-alanine-activating enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002688264.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTA AATTCAAGTC TTCCTATGAA GCATTATTGA ACTATGATAT GCTTAAAGTA
    GAACATAATG ACCTAGTACT CTTCAGACTT CACTGGAAAA ATGTCGAGGT GAGCCTGGTG
    CTAAATGATA GAAAAGAGAA ATATGAAAAA GGAAAGATGC CAAAGAGTAT GCATTCTGAG
    AGCAGCAGTG AAGAAAAGTC AGAAGAGCAC ATGGATATGA GGCAAAAGCA CTGCTTGGCC
    TATGTTCTCC ATACGTCAGG CACTACAGGG ATACCTAAGA TTGTCAGAGT CCCTCATGCA
    TGTATAGTGC CAAACATCCA GCATTTTCGG GTGCTTTTTG AAATCACTCA TGAGGATGTT
    TTGTTTCTGG CTTCACCTCT GACCTTTGAT CCTTCTGTGG TGGAAATATT TGTTGCTTTG
    TCAAGTGGTG CCTCTTTGCT TATTGTGCCA ACTTCTGTCA AAATGGTCCC ATCAAAATTA
    GCTGCTGTTC TGTTTTCCCA TCACAGAGTG ACTATTTTAC AGGCAACGCC AACATTGCTT
    AGAAGATTTG GATCTCAGCT CATCAAGTCA ACTGTTTTAT CCGCCAGTAC CTCTCTTCGC
    ATATTGGCCC TTGGTGGTGA AGCATTTCCA TCATTAACTG TTCTCAAAAG CTGGAGAGGA
    GTGGGCAATA AAACACAAAT ATTTAATATT TATGGCATCA CAGAGGTATC AAGTTGGGCG
    ACTTTCTACA GGATTCCAGA GAAGATTCTT AACTCTACTT TGAAATGTGA ATTGCCTGTA
    CAACTGGGAT TCCCACTGCT TGGAACAGTA GTTGAAGTCA GAGATACTAA CGGTTTCACA
    ATTCAAGAAG GCAATGGCCA AGTATTTTTA GGTGGCAGAA ATAGAGTATG TTTTCTCGAT
    GGCGAAGTGA CGGTACCACT TGGCACAATG AGAGCCACAG GAGACTTTGT GACTATAAAA
    GATGGAGAGA TATTTTTCTT GGGACGAAAG GACAGTCAGA TCAAACGTCA TGGCAAACGT
    CTTAACATAG AACTTGTACA ACAGATTGCT GAAGGTCTTC AACAAGTGGA GTCTTGTGCA
    GTTACATGGT ATAACCAGGA AAAATTGATT TTGTTCATGG TGACTAAAAA TGATTTAGTA
    AAGGATCACA TCTTTGAAGA ACTGCAGAAA CATCTTCCAA GTCATGCCAT CCCAGATGAG
    CTTGTGTTAA TTAATTCTCT GCCATTTACA TCTCATGGCA AAATTGATGT TTCTGAGTTA
    AATAAGATAT ATTTAAACTA CAGAGACTTG AAGTCTGAGT GTAAGCTCAG TGGAAAAGAG
    GAACTTTGGG AAAAATTACA CCATTTGTGG AAGTCTGTTC TGAGTCTCTC AGAAGATGCT
    TTGAAGATTC CTGATGAGTC ACTCTTCTTA AATAGTGGTG GAGATTCCTT GAAATCCATA
    CGGCTTCTCA ATGAGATTGA AAACCTTGTT GGCACATCAG TACCTGGGCT TCTGGAAATT
    ATTCTCAGCA GTTCTATTTT ACGGATTTAC AATCACATCC TTCAAACAGT GTTTTCAGAT
    AAAGATATGA CACTTAGCAA GAATCATGCC ATGAAAAGAA AATTCAACGT TACTCAAGAG
    GAAACCAGTG GAAAATCTTT ACATCAGGAA ACTGTCATGC CTTTAAATTG TGACAAGGAG
    ATCAATGCTT TTATTGCACT GAGCAGAGGG AATCAGATTT TGTCTTTGAA TACTGATAAG
    TATTTAACTA AGTTGGGACA TTGCCCTTCA ACCTATTCTT CTGATTTAAA TTCACAGACT
    GACATTCAAA ATAGAGAAAG CCTAAATACT CCACCTCTTA TTGGGAAGTC AGAAGATCCA
    TCCTGTGTTG TGGAAGTTTC TGAAGAGGAA ACACCTGTGA TTAGGGCTGA GAAAATGGAG
    TTTCGTGTGA GGTGGAGGTC AGACACAGGC AAATGTGTGG ATGCTTCACC TCTGGTTGTA
    ATACCAGCTA TTGATAAGTC ATCTGCAACT GTGTACATTG GCTCTCATTC TCATAGAATG
    ATAGCAGTTG ATCTTTACTC TGGAAAGGTG AAATGGGAAC AGATTTTAGG AGATCGCATT
    GAATCCTCAG CATGTGTATC TAAGTGTGGA AACTTTATTG TAGTGGGCTG TTATAATGGG
    TTAGTTTATG TTCTGAAAAG TAATAGTGGA GAAAAATACT GGATGTTTAG TACTGAGGAT
    GCTGTCAAAA GCTCAGCAAC CATGGATCCA ACCACAGGAC TTCTTTACAT TGGATCTCAT
    GACCAGCATG CATACGCTTT GGATATATAT AAGAAGAAGT GTGTTTGGAA GTTGAAATGT
    GGAGGAACTG TTTTTTCTTC TCCTTGTTTG AGCCTGATTC CATCTCACTT GTATTTTGCT
    ACACTGGGAG GACTTTTACT GGCTATAAAT CCTGCCACTG GGGACAGACT TTGGAAACAT
    TCCTGTGGAA AGCCACTCTT TTCTTCTCCA CGGTGTTGCC TACAGTATGT TTTTATTGGC
    TGTGTAGATG GAAATTTACT CTGCTTTACT CACTTTGGAG AACAGGTTTG GCAATTCTCT
    ACCAATGAAC CAATCTTTTC ATCCCCATGT ACCTCAGCAT CAGAGCAAGA AATATTTTTT
    GGTTCTCATG ACTGCTTTAT CTACTGTTGT AGTATGAAAG GTCACCTCCA GTGGAAATTT
    GAAACTACTT CAAGAGTGTA TTCAACGCCT TTTGTTTTCC GTAACCACAA CCATAACAGT
    GAAATGTTAC TGGCAGCGGC ATCTACTGAT GGGAAACTGT GGATCTTGGA ATCTAAGAGT
    GGACAGTTGC AAAGTGTGTA TGAACTTCCT GGAGAAGTTT TCTCTTCTCC TGTGGTCTGG
    GAATCAATGC TTATTATTGG ATGTAGAAAT AATTATGTTT ATTGTCTGGA TGTATTGGGT
    GACAAAGGTA ATGAAGTGCA GTCCTTAGAA TATACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002688310.2
    CDS450..3848
    Translation

    Target ORF information:

    RefSeq Version XM_002688264.6
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002688264.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTA AATTCAAGTC TTCCTATGAA GCATTATTGA ACTATGATAT GCTTAAAGTA
    GAACATAATG ACCTAGTACT CTTCAGACTT CACTGGAAAA ATGTCGAGGT GAGCCTGGTG
    CTAAATGATA GAAAAGAGAA ATATGAAAAA GGAAAGATGC CAAAGAGTAT GCATTCTGAG
    AGCAGCAGTG AAGAAAAGTC AGAAGAGCAC ATGGATATGA GGCAAAAGCA CTGCTTGGCC
    TATGTTCTCC ATACGTCAGG CACTACAGGG ATACCTAAGA TTGTCAGAGT CCCTCATGCA
    TGTATAGTGC CAAACATCCA GCATTTTCGG GTGCTTTTTG AAATCACTCA TGAGGATGTT
    TTGTTTCTGG CTTCACCTCT GACCTTTGAT CCTTCTGTGG TGGAAATATT TGTTGCTTTG
    TCAAGTGGTG CCTCTTTGCT TATTGTGCCA ACTTCTGTCA AAATGGTCCC ATCAAAATTA
    GCTGCTGTTC TGTTTTCCCA TCACAGAGTG ACTATTTTAC AGGCAACGCC AACATTGCTT
    AGAAGATTTG GATCTCAGCT CATCAAGTCA ACTGTTTTAT CCGCCAGTAC CTCTCTTCGC
    ATATTGGCCC TTGGTGGTGA AGCATTTCCA TCATTAACTG TTCTCAAAAG CTGGAGAGGA
    GTGGGCAATA AAACACAAAT ATTTAATATT TATGGCATCA CAGAGGTATC AAGTTGGGCG
    ACTTTCTACA GGATTCCAGA GAAGATTCTT AACTCTACTT TGAAATGTGA ATTGCCTGTA
    CAACTGGGAT TCCCACTGCT TGGAACAGTA GTTGAAGTCA GAGATACTAA CGGTTTCACA
    ATTCAAGAAG GCAATGGCCA AGTATTTTTA GGTGGCAGAA ATAGAGTATG TTTTCTCGAT
    GGCGAAGTGA CGGTACCACT TGGCACAATG AGAGCCACAG GAGACTTTGT GACTATAAAA
    GATGGAGAGA TATTTTTCTT GGGACGAAAG GACAGTCAGA TCAAACGTCA TGGCAAACGT
    CTTAACATAG AACTTGTACA ACAGATTGCT GAAGGTCTTC AACAAGTGGA GTCTTGTGCA
    GTTACATGGT ATAACCAGGA AAAATTGATT TTGTTCATGG TGACTAAAAA TGATTTAGTA
    AAGGATCACA TCTTTGAAGA ACTGCAGAAA CATCTTCCAA GTCATGCCAT CCCAGATGAG
    CTTGTGTTAA TTAATTCTCT GCCATTTACA TCTCATGGCA AAATTGATGT TTCTGAGTTA
    AATAAGATAT ATTTAAACTA CAGAGACTTG AAGTCTGAGT GTAAGCTCAG TGGAAAAGAG
    GAACTTTGGG AAAAATTACA CCATTTGTGG AAGTCTGTTC TGAGTCTCTC AGAAGATGCT
    TTGAAGATTC CTGATGAGTC ACTCTTCTTA AATAGTGGTG GAGATTCCTT GAAATCCATA
    CGGCTTCTCA ATGAGATTGA AAACCTTGTT GGCACATCAG TACCTGGGCT TCTGGAAATT
    ATTCTCAGCA GTTCTATTTT ACGGATTTAC AATCACATCC TTCAAACAGT GTTTTCAGAT
    AAAGATATGA CACTTAGCAA GAATCATGCC ATGAAAAGAA AATTCAACGT TACTCAAGAG
    GAAACCAGTG GAAAATCTTT ACATCAGGAA ACTGTCATGC CTTTAAATTG TGACAAGGAG
    ATCAATGCTT TTATTGCACT GAGCAGAGGG AATCAGATTT TGTCTTTGAA TACTGATAAG
    TATTTAACTA AGTTGGGACA TTGCCCTTCA ACCTATTCTT CTGATTTAAA TTCACAGACT
    GACATTCAAA ATAGAGAAAG CCTAAATACT CCACCTCTTA TTGGGAAGTC AGAAGATCCA
    TCCTGTGTTG TGGAAGTTTC TGAAGAGGAA ACACCTGTGA TTAGGGCTGA GAAAATGGAG
    TTTCGTGTGA GGTGGAGGTC AGACACAGGC AAATGTGTGG ATGCTTCACC TCTGGTTGTA
    ATACCAGCTA TTGATAAGTC ATCTGCAACT GTGTACATTG GCTCTCATTC TCATAGAATG
    ATAGCAGTTG ATCTTTACTC TGGAAAGGTG AAATGGGAAC AGATTTTAGG AGATCGCATT
    GAATCCTCAG CATGTGTATC TAAGTGTGGA AACTTTATTG TAGTGGGCTG TTATAATGGG
    TTAGTTTATG TTCTGAAAAG TAATAGTGGA GAAAAATACT GGATGTTTAG TACTGAGGAT
    GCTGTCAAAA GCTCAGCAAC CATGGATCCA ACCACAGGAC TTCTTTACAT TGGATCTCAT
    GACCAGCATG CATACGCTTT GGATATATAT AAGAAGAAGT GTGTTTGGAA GTTGAAATGT
    GGAGGAACTG TTTTTTCTTC TCCTTGTTTG AGCCTGATTC CATCTCACTT GTATTTTGCT
    ACACTGGGAG GACTTTTACT GGCTATAAAT CCTGCCACTG GGGACAGACT TTGGAAACAT
    TCCTGTGGAA AGCCACTCTT TTCTTCTCCA CGGTGTTGCC TACAGTATGT TTTTATTGGC
    TGTGTAGATG GAAATTTACT CTGCTTTACT CACTTTGGAG AACAGGTTTG GCAATTCTCT
    ACCAATGAAC CAATCTTTTC ATCCCCATGT ACCTCAGCAT CAGAGCAAGA AATATTTTTT
    GGTTCTCATG ACTGCTTTAT CTACTGTTGT AGTATGAAAG GTCACCTCCA GTGGAAATTT
    GAAACTACTT CAAGAGTGTA TTCAACGCCT TTTGTTTTCC GTAACCACAA CCATAACAGT
    GAAATGTTAC TGGCAGCGGC ATCTACTGAT GGGAAACTGT GGATCTTGGA ATCTAAGAGT
    GGACAGTTGC AAAGTGTGTA TGAACTTCCT GGAGAAGTTT TCTCTTCTCC TGTGGTCTGG
    GAATCAATGC TTATTATTGG ATGTAGAAAT AATTATGTTT ATTGTCTGGA TGTATTGGGT
    GACAAAGGTA ATGAAGTGCA GTCCTTAGAA TATACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235564
    Clone ID Related Accession (Same CDS sequence) XM_024993575.1
    Accession Version XM_024993575.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product beta-alanine-activating enzyme isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTA AATTCAAGTC TTCCTATGAA GCATTATTGA ACTATGATAT GCTTAAAGTA
    GAACATAATG ACCTAGTACT CTTCAGACTT CACTGGAAAA ATGTCGAGGT GAGCCTGGTG
    CTAAATGATA GAAAAGAGAA ATATGAAAAA GGAAAGATGC CAAAGAGTAT GCATTCTGAG
    AGCAGCAGTG AAGAAAAGTC AGAAGAGCAC ATGGATATGA GGCAAAAGCA CTGCTTGGCC
    TATGTTCTCC ATACGTCAGG CACTACAGGG ATACCTAAGA TTGTCAGAGT CCCTCATGCA
    TGTATAGTGC CAAACATCCA GCATTTTCGG GTGCTTTTTG AAATCACTCA TGAGGATGTT
    TTGTTTCTGG CTTCACCTCT GACCTTTGAT CCTTCTGTGG TGGAAATATT TGTTGCTTTG
    TCAAGTGGTG CCTCTTTGCT TATTGTGCCA ACTTCTGTCA AAATGGTCCC ATCAAAATTA
    GCTGCTGTTC TGTTTTCCCA TCACAGAGTG ACTATTTTAC AGGCAACGCC AACATTGCTT
    AGAAGATTTG GATCTCAGCT CATCAAGTCA ACTGTTTTAT CCGCCAGTAC CTCTCTTCGC
    ATATTGGCCC TTGGTGGTGA AGCATTTCCA TCATTAACTG TTCTCAAAAG CTGGAGAGGA
    GTGGGCAATA AAACACAAAT ATTTAATATT TATGGCATCA CAGAGGTATC AAGTTGGGCG
    ACTTTCTACA GGATTCCAGA GAAGATTCTT AACTCTACTT TGAAATGTGA ATTGCCTGTA
    CAACTGGGAT TCCCACTGCT TGGAACAGTA GTTGAAGTCA GAGATACTAA CGGTTTCACA
    ATTCAAGAAG GCAATGGCCA AGTATTTTTA GGTGGCAGAA ATAGAGTATG TTTTCTCGAT
    GGCGAAGTGA CGGTACCACT TGGCACAATG AGAGCCACAG GAGACTTTGT GACTATAAAA
    GATGGAGAGA TATTTTTCTT GGGACGAAAG GACAGTCAGA TCAAACGTCA TGGCAAACGT
    CTTAACATAG AACTTGTACA ACAGATTGCT GAAGGTCTTC AACAAGTGGA GTCTTGTGCA
    GTTACATGGT ATAACCAGGA AAAATTGATT TTGTTCATGG TGACTAAAAA TGATTTAGTA
    AAGGATCACA TCTTTGAAGA ACTGCAGAAA CATCTTCCAA GTCATGCCAT CCCAGATGAG
    CTTGTGTTAA TTAATTCTCT GCCATTTACA TCTCATGGCA AAATTGATGT TTCTGAGTTA
    AATAAGATAT ATTTAAACTA CAGAGACTTG AAGTCTGAGT GTAAGCTCAG TGGAAAAGAG
    GAACTTTGGG AAAAATTACA CCATTTGTGG AAGTCTGTTC TGAGTCTCTC AGAAGATGCT
    TTGAAGATTC CTGATGAGTC ACTCTTCTTA AATAGTGGTG GAGATTCCTT GAAATCCATA
    CGGCTTCTCA ATGAGATTGA AAACCTTGTT GGCACATCAG TACCTGGGCT TCTGGAAATT
    ATTCTCAGCA GTTCTATTTT ACGGATTTAC AATCACATCC TTCAAACAGT GTTTTCAGAT
    AAAGATATGA CACTTAGCAA GAATCATGCC ATGAAAAGAA AATTCAACGT TACTCAAGAG
    GAAACCAGTG GAAAATCTTT ACATCAGGAA ACTGTCATGC CTTTAAATTG TGACAAGGAG
    ATCAATGCTT TTATTGCACT GAGCAGAGGG AATCAGATTT TGTCTTTGAA TACTGATAAG
    TATTTAACTA AGTTGGGACA TTGCCCTTCA ACCTATTCTT CTGATTTAAA TTCACAGACT
    GACATTCAAA ATAGAGAAAG CCTAAATACT CCACCTCTTA TTGGGAAGTC AGAAGATCCA
    TCCTGTGTTG TGGAAGTTTC TGAAGAGGAA ACACCTGTGA TTAGGGCTGA GAAAATGGAG
    TTTCGTGTGA GGTGGAGGTC AGACACAGGC AAATGTGTGG ATGCTTCACC TCTGGTTGTA
    ATACCAGCTA TTGATAAGTC ATCTGCAACT GTGTACATTG GCTCTCATTC TCATAGAATG
    ATAGCAGTTG ATCTTTACTC TGGAAAGGTG AAATGGGAAC AGATTTTAGG AGATCGCATT
    GAATCCTCAG CATGTGTATC TAAGTGTGGA AACTTTATTG TAGTGGGCTG TTATAATGGG
    TTAGTTTATG TTCTGAAAAG TAATAGTGGA GAAAAATACT GGATGTTTAG TACTGAGGAT
    GCTGTCAAAA GCTCAGCAAC CATGGATCCA ACCACAGGAC TTCTTTACAT TGGATCTCAT
    GACCAGCATG CATACGCTTT GGATATATAT GCCACTGGGG ACAGACTTTG GAAACATTCC
    TGTGGAAAGC CACTCTTTTC TTCTCCACGG TGTTGCCTAC AGTATGTTTT TATTGGCTGT
    GTAGATGGAA ATTTACTCTG CTTTACTCAC TTTGGAGAAC AGGTTTGGCA ATTCTCTACC
    AATGAACCAA TCTTTTCATC CCCATGTACC TCAGCATCAG AGCAAGAAAT ATTTTTTGGT
    TCTCATGACT GCTTTATCTA CTGTTGTAGT ATGAAAGGTC ACCTCCAGTG GAAATTTGAA
    ACTACTTCAA GAGTGTATTC AACGCCTTTT GTTTTCCGTA ACCACAACCA TAACAGTGAA
    ATGTTACTGG CAGCGGCATC TACTGATGGG AAACTGTGGA TCTTGGAATC TAAGAGTGGA
    CAGTTGCAAA GTGTGTATGA ACTTCCTGGA GAAGTTTTCT CTTCTCCTGT GGTCTGGGAA
    TCAATGCTTA TTATTGGATG TAGAAATAAT TATGTTTATT GTCTGGATGT ATTGGGTGAC
    AAAGGTAATG AAGTGCAGTC CTTAGAATAT ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849343.1
    CDS452..3727
    Translation

    Target ORF information:

    RefSeq Version XM_024993575.1
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993575.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTA AATTCAAGTC TTCCTATGAA GCATTATTGA ACTATGATAT GCTTAAAGTA
    GAACATAATG ACCTAGTACT CTTCAGACTT CACTGGAAAA ATGTCGAGGT GAGCCTGGTG
    CTAAATGATA GAAAAGAGAA ATATGAAAAA GGAAAGATGC CAAAGAGTAT GCATTCTGAG
    AGCAGCAGTG AAGAAAAGTC AGAAGAGCAC ATGGATATGA GGCAAAAGCA CTGCTTGGCC
    TATGTTCTCC ATACGTCAGG CACTACAGGG ATACCTAAGA TTGTCAGAGT CCCTCATGCA
    TGTATAGTGC CAAACATCCA GCATTTTCGG GTGCTTTTTG AAATCACTCA TGAGGATGTT
    TTGTTTCTGG CTTCACCTCT GACCTTTGAT CCTTCTGTGG TGGAAATATT TGTTGCTTTG
    TCAAGTGGTG CCTCTTTGCT TATTGTGCCA ACTTCTGTCA AAATGGTCCC ATCAAAATTA
    GCTGCTGTTC TGTTTTCCCA TCACAGAGTG ACTATTTTAC AGGCAACGCC AACATTGCTT
    AGAAGATTTG GATCTCAGCT CATCAAGTCA ACTGTTTTAT CCGCCAGTAC CTCTCTTCGC
    ATATTGGCCC TTGGTGGTGA AGCATTTCCA TCATTAACTG TTCTCAAAAG CTGGAGAGGA
    GTGGGCAATA AAACACAAAT ATTTAATATT TATGGCATCA CAGAGGTATC AAGTTGGGCG
    ACTTTCTACA GGATTCCAGA GAAGATTCTT AACTCTACTT TGAAATGTGA ATTGCCTGTA
    CAACTGGGAT TCCCACTGCT TGGAACAGTA GTTGAAGTCA GAGATACTAA CGGTTTCACA
    ATTCAAGAAG GCAATGGCCA AGTATTTTTA GGTGGCAGAA ATAGAGTATG TTTTCTCGAT
    GGCGAAGTGA CGGTACCACT TGGCACAATG AGAGCCACAG GAGACTTTGT GACTATAAAA
    GATGGAGAGA TATTTTTCTT GGGACGAAAG GACAGTCAGA TCAAACGTCA TGGCAAACGT
    CTTAACATAG AACTTGTACA ACAGATTGCT GAAGGTCTTC AACAAGTGGA GTCTTGTGCA
    GTTACATGGT ATAACCAGGA AAAATTGATT TTGTTCATGG TGACTAAAAA TGATTTAGTA
    AAGGATCACA TCTTTGAAGA ACTGCAGAAA CATCTTCCAA GTCATGCCAT CCCAGATGAG
    CTTGTGTTAA TTAATTCTCT GCCATTTACA TCTCATGGCA AAATTGATGT TTCTGAGTTA
    AATAAGATAT ATTTAAACTA CAGAGACTTG AAGTCTGAGT GTAAGCTCAG TGGAAAAGAG
    GAACTTTGGG AAAAATTACA CCATTTGTGG AAGTCTGTTC TGAGTCTCTC AGAAGATGCT
    TTGAAGATTC CTGATGAGTC ACTCTTCTTA AATAGTGGTG GAGATTCCTT GAAATCCATA
    CGGCTTCTCA ATGAGATTGA AAACCTTGTT GGCACATCAG TACCTGGGCT TCTGGAAATT
    ATTCTCAGCA GTTCTATTTT ACGGATTTAC AATCACATCC TTCAAACAGT GTTTTCAGAT
    AAAGATATGA CACTTAGCAA GAATCATGCC ATGAAAAGAA AATTCAACGT TACTCAAGAG
    GAAACCAGTG GAAAATCTTT ACATCAGGAA ACTGTCATGC CTTTAAATTG TGACAAGGAG
    ATCAATGCTT TTATTGCACT GAGCAGAGGG AATCAGATTT TGTCTTTGAA TACTGATAAG
    TATTTAACTA AGTTGGGACA TTGCCCTTCA ACCTATTCTT CTGATTTAAA TTCACAGACT
    GACATTCAAA ATAGAGAAAG CCTAAATACT CCACCTCTTA TTGGGAAGTC AGAAGATCCA
    TCCTGTGTTG TGGAAGTTTC TGAAGAGGAA ACACCTGTGA TTAGGGCTGA GAAAATGGAG
    TTTCGTGTGA GGTGGAGGTC AGACACAGGC AAATGTGTGG ATGCTTCACC TCTGGTTGTA
    ATACCAGCTA TTGATAAGTC ATCTGCAACT GTGTACATTG GCTCTCATTC TCATAGAATG
    ATAGCAGTTG ATCTTTACTC TGGAAAGGTG AAATGGGAAC AGATTTTAGG AGATCGCATT
    GAATCCTCAG CATGTGTATC TAAGTGTGGA AACTTTATTG TAGTGGGCTG TTATAATGGG
    TTAGTTTATG TTCTGAAAAG TAATAGTGGA GAAAAATACT GGATGTTTAG TACTGAGGAT
    GCTGTCAAAA GCTCAGCAAC CATGGATCCA ACCACAGGAC TTCTTTACAT TGGATCTCAT
    GACCAGCATG CATACGCTTT GGATATATAT GCCACTGGGG ACAGACTTTG GAAACATTCC
    TGTGGAAAGC CACTCTTTTC TTCTCCACGG TGTTGCCTAC AGTATGTTTT TATTGGCTGT
    GTAGATGGAA ATTTACTCTG CTTTACTCAC TTTGGAGAAC AGGTTTGGCA ATTCTCTACC
    AATGAACCAA TCTTTTCATC CCCATGTACC TCAGCATCAG AGCAAGAAAT ATTTTTTGGT
    TCTCATGACT GCTTTATCTA CTGTTGTAGT ATGAAAGGTC ACCTCCAGTG GAAATTTGAA
    ACTACTTCAA GAGTGTATTC AACGCCTTTT GTTTTCCGTA ACCACAACCA TAACAGTGAA
    ATGTTACTGG CAGCGGCATC TACTGATGGG AAACTGTGGA TCTTGGAATC TAAGAGTGGA
    CAGTTGCAAA GTGTGTATGA ACTTCCTGGA GAAGTTTTCT CTTCTCCTGT GGTCTGGGAA
    TCAATGCTTA TTATTGGATG TAGAAATAAT TATGTTTATT GTCTGGATGT ATTGGGTGAC
    AAAGGTAATG AAGTGCAGTC CTTAGAATAT ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235565
    Clone ID Related Accession (Same CDS sequence) XM_024993576.1
    Accession Version XM_024993576.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3021bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product beta-alanine-activating enzyme isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAAAAAAT GTAATCTGAA GTATATCCTT GTTGAAAAAG TGCATATAAG TAAATTCAAG 
    TCTTCCTATG AAGCATTATT GAACTATGAT ATGCTTAAAG TAGAACATAA TGACCTAGTA
    CTCTTCAGAC TTCACTGGAA AAATGTCGAG GTGAGCCTGG TGCTAAATGA TAGAAAAGAG
    AAATATGAAA AAGGAAAGAT GCCAAAGAGT ATGCATTCTG AGAGCAGCAG TGAAGAAAAG
    TCAGAAGAGC ACATGGATAT GAGGCAAAAG CACTGCTTGG CCTATGTTCT CCATACGTCA
    GGCACTACAG GGATACCTAA GATTGTCAGA GTCCCTCATG CATGTATAGT GCCAAACATC
    CAGCATTTTC GGGTGCTTTT TGAAATCACT CATGAGGATG TTTTGTTTCT GGCTTCACCT
    CTGACCTTTG ATCCTTCTGT GGTGGAAATA TTTGTTGCTT TGTCAAGTGG TGCCTCTTTG
    CTTATTGTGC CAACTTCTGT CAAAATGGTC CCATCAAAAT TAGCTGCTGT TCTGTTTTCC
    CATCACAGAG TGACTATTTT ACAGGCAACG CCAACATTGC TTAGAAGATT TGGATCTCAG
    CTCATCAAGT CAACTGTTTT ATCCGCCAGT ACCTCTCTTC GCATATTGGC CCTTGGTGGT
    GAAGCATTTC CATCATTAAC TGTTCTCAAA AGCTGGAGAG GAGTGGGCAA TAAAACACAA
    ATATTTAATA TTTATGGCAT CACAGAGGTA TCAAGTTGGG CGACTTTCTA CAGGATTCCA
    GAGAAGATTC TTAACTCTAC TTTGAAATGT GAATTGCCTG TACAACTGGG ATTCCCACTG
    CTTGGAACAG TAGTTGAAGT CAGAGATACT AACGGTTTCA CAATTCAAGA AGGCAATGGC
    CAAGTATTTT TAGGTGGCAG AAATAGAGTA TGTTTTCTCG ATGGCGAAGT GACGGTACCA
    CTTGGCACAA TGAGAGCCAC AGGAGACTTT GTGACTATAA AAGATGGAGA GATATTTTTC
    TTGGGACGAA AGGACAGTCA GATCAAACGT CATGGCAAAC GTCTTAACAT AGAACTTGTA
    CAACAGATTG CTGAAGGTCT TCAACAAGTG GAGTCTTGTG CAGTTACATG GTATAACCAG
    GAAAAATTGA TTTTGTTCAT GGTGACTAAA AATGATTTAG TAAAGGATCA CATCTTTGAA
    GAACTGCAGA AACATCTTCC AAGTCATGCC ATCCCAGATG AGCTTGTGTT AATTAATTCT
    CTGCCATTTA CATCTCATGG CAAAATTGAT GTTTCTGAGT TAAATAAGAT ATATTTAAAC
    TACAGAGACT TGAAGTCTGA GTGTAAGCTC AGTGGAAAAG AGGAACTTTG GGAAAAATTA
    CACCATTTGT GGAAGTCTGT TCTGAGTCTC TCAGAAGATG CTTTGAAGAT TCCTGATGAG
    TCACTCTTCT TAAATAGTGG TGGAGATTCC TTGAAATCCA TACGGCTTCT CAATGAGATT
    GAAAACCTTG TTGGCACATC AGTACCTGGG CTTCTGGAAA TTATTCTCAG CAGTTCTATT
    TTACGGATTT ACAATCACAT CCTTCAAACA GTGTTTTCAG ATAAAGATAT GACACTTAGC
    AAGAATCATG CCATGAAAAG AAAATTCAAC GTTACTCAAG AGGAAACCAG TGGAAAATCT
    TTACATCAGG AAACTGTCAT GCCTTTAAAT TGTGACAAGG AGATCAATGC TTTTATTGCA
    CTGAGCAGAG GGAATCAGAT TTTGTCTTTG AATACTGATA AGTATTTAAC TAAGTTGGGA
    CATTGCCCTT CAACCTATTC TTCTGATTTA AATTCACAGA CTGACATTCA AAATAGAGAA
    AGCCTAAATA CTCCACCTCT TATTGGGAAG TCAGAAGATC CATCCTGTGT TGTGGAAGTT
    TCTGAAGAGG AAACACCTGT GATTAGGGCT GAGAAAATGG AGTTTCGTGT GAGGTGGAGG
    TCAGACACAG GCAAATGTGT GGATGCTTCA CCTCTGGTTG TAATACCAGC TATTGATAAG
    TCATCTGCAA CTGTGTACAT TGGCTCTCAT TCTCATAGAA TGATAGCAGT TGATCTTTAC
    TCTGGAAAGG TGAAATGGGA ACAGATTTTA GGAGATCGCA TTGAATCCTC AGCATGTGTA
    TCTAAGTGTG GAAACTTTAT TGTAGTGGGC TGTTATAATG GGTTAGTTTA TGTTCTGAAA
    AGTAATAGTG GAGAAAAATA CTGGATGTTT AGTACTGAGG ATGCTGTCAA AAGCTCAGCA
    ACCATGGATC CAACCACAGG ACTTCTTTAC ATTGGATCTC ATGACCAGCA TGCATACGCT
    TTGGATATAT ATAAGAAGAA GTGTGTTTGG AAGTTGAAAT GTGGAGGAAC TGTTTTTTCT
    TCTCCTTGTT TGAGCCTGAT TCCATCTCAC TTGTATTTTG CTACACTGGG AGGACTTTTA
    CTGGCTATAA ATCCTGCCAC TGGGGACAGA CTTTGGAAAC ATTCCTGTGG AAAGCCACTC
    TTTTCTTCTC CACGGTGTTG CCTACAGTAT GTTTTTATTG GCTGTGTAGA TGGAAATTTA
    CTCTGCTTTA CTCACTTTGG AGAACAGGTT TGGCAATTCT CTACCAATGA ACCAATCTTT
    TCATCCCCAT GTACCTCAGC ATCAGAGCAA GAAATATTTT TTGGTTCTCA TGACTGCTTT
    ATCTACTGTT GTAGTATGAA AGGTCACCTC CAGTGGAAAT TTGAAACTAC TTCAAGAGTG
    TATTCAACGC CTTTTGTTTT CCGTAACCAC AACCATAACA GTGAAATGTT ACTGGCAGCG
    GCATCTACTG ATGGGAAACT GTGGATCTTG GAATCTAAGA GTGGACAGTT GCAAAGTGTG
    TATGAACTTC CTGGAGAAGT TTTCTCTTCT CCTGTGGTCT GGGAATCAAT GCTTATTATT
    GGATGTAGAA ATAATTATGT TTATTGTCTG GATGTATTGG GTGACAAAGG TAATGAAGTG
    CAGTCCTTAG AATATACATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849344.1
    CDS557..3577
    Translation

    Target ORF information:

    RefSeq Version XM_024993576.1
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993576.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAAAAAAT GTAATCTGAA GTATATCCTT GTTGAAAAAG TGCATATAAG TAAATTCAAG 
    TCTTCCTATG AAGCATTATT GAACTATGAT ATGCTTAAAG TAGAACATAA TGACCTAGTA
    CTCTTCAGAC TTCACTGGAA AAATGTCGAG GTGAGCCTGG TGCTAAATGA TAGAAAAGAG
    AAATATGAAA AAGGAAAGAT GCCAAAGAGT ATGCATTCTG AGAGCAGCAG TGAAGAAAAG
    TCAGAAGAGC ACATGGATAT GAGGCAAAAG CACTGCTTGG CCTATGTTCT CCATACGTCA
    GGCACTACAG GGATACCTAA GATTGTCAGA GTCCCTCATG CATGTATAGT GCCAAACATC
    CAGCATTTTC GGGTGCTTTT TGAAATCACT CATGAGGATG TTTTGTTTCT GGCTTCACCT
    CTGACCTTTG ATCCTTCTGT GGTGGAAATA TTTGTTGCTT TGTCAAGTGG TGCCTCTTTG
    CTTATTGTGC CAACTTCTGT CAAAATGGTC CCATCAAAAT TAGCTGCTGT TCTGTTTTCC
    CATCACAGAG TGACTATTTT ACAGGCAACG CCAACATTGC TTAGAAGATT TGGATCTCAG
    CTCATCAAGT CAACTGTTTT ATCCGCCAGT ACCTCTCTTC GCATATTGGC CCTTGGTGGT
    GAAGCATTTC CATCATTAAC TGTTCTCAAA AGCTGGAGAG GAGTGGGCAA TAAAACACAA
    ATATTTAATA TTTATGGCAT CACAGAGGTA TCAAGTTGGG CGACTTTCTA CAGGATTCCA
    GAGAAGATTC TTAACTCTAC TTTGAAATGT GAATTGCCTG TACAACTGGG ATTCCCACTG
    CTTGGAACAG TAGTTGAAGT CAGAGATACT AACGGTTTCA CAATTCAAGA AGGCAATGGC
    CAAGTATTTT TAGGTGGCAG AAATAGAGTA TGTTTTCTCG ATGGCGAAGT GACGGTACCA
    CTTGGCACAA TGAGAGCCAC AGGAGACTTT GTGACTATAA AAGATGGAGA GATATTTTTC
    TTGGGACGAA AGGACAGTCA GATCAAACGT CATGGCAAAC GTCTTAACAT AGAACTTGTA
    CAACAGATTG CTGAAGGTCT TCAACAAGTG GAGTCTTGTG CAGTTACATG GTATAACCAG
    GAAAAATTGA TTTTGTTCAT GGTGACTAAA AATGATTTAG TAAAGGATCA CATCTTTGAA
    GAACTGCAGA AACATCTTCC AAGTCATGCC ATCCCAGATG AGCTTGTGTT AATTAATTCT
    CTGCCATTTA CATCTCATGG CAAAATTGAT GTTTCTGAGT TAAATAAGAT ATATTTAAAC
    TACAGAGACT TGAAGTCTGA GTGTAAGCTC AGTGGAAAAG AGGAACTTTG GGAAAAATTA
    CACCATTTGT GGAAGTCTGT TCTGAGTCTC TCAGAAGATG CTTTGAAGAT TCCTGATGAG
    TCACTCTTCT TAAATAGTGG TGGAGATTCC TTGAAATCCA TACGGCTTCT CAATGAGATT
    GAAAACCTTG TTGGCACATC AGTACCTGGG CTTCTGGAAA TTATTCTCAG CAGTTCTATT
    TTACGGATTT ACAATCACAT CCTTCAAACA GTGTTTTCAG ATAAAGATAT GACACTTAGC
    AAGAATCATG CCATGAAAAG AAAATTCAAC GTTACTCAAG AGGAAACCAG TGGAAAATCT
    TTACATCAGG AAACTGTCAT GCCTTTAAAT TGTGACAAGG AGATCAATGC TTTTATTGCA
    CTGAGCAGAG GGAATCAGAT TTTGTCTTTG AATACTGATA AGTATTTAAC TAAGTTGGGA
    CATTGCCCTT CAACCTATTC TTCTGATTTA AATTCACAGA CTGACATTCA AAATAGAGAA
    AGCCTAAATA CTCCACCTCT TATTGGGAAG TCAGAAGATC CATCCTGTGT TGTGGAAGTT
    TCTGAAGAGG AAACACCTGT GATTAGGGCT GAGAAAATGG AGTTTCGTGT GAGGTGGAGG
    TCAGACACAG GCAAATGTGT GGATGCTTCA CCTCTGGTTG TAATACCAGC TATTGATAAG
    TCATCTGCAA CTGTGTACAT TGGCTCTCAT TCTCATAGAA TGATAGCAGT TGATCTTTAC
    TCTGGAAAGG TGAAATGGGA ACAGATTTTA GGAGATCGCA TTGAATCCTC AGCATGTGTA
    TCTAAGTGTG GAAACTTTAT TGTAGTGGGC TGTTATAATG GGTTAGTTTA TGTTCTGAAA
    AGTAATAGTG GAGAAAAATA CTGGATGTTT AGTACTGAGG ATGCTGTCAA AAGCTCAGCA
    ACCATGGATC CAACCACAGG ACTTCTTTAC ATTGGATCTC ATGACCAGCA TGCATACGCT
    TTGGATATAT ATAAGAAGAA GTGTGTTTGG AAGTTGAAAT GTGGAGGAAC TGTTTTTTCT
    TCTCCTTGTT TGAGCCTGAT TCCATCTCAC TTGTATTTTG CTACACTGGG AGGACTTTTA
    CTGGCTATAA ATCCTGCCAC TGGGGACAGA CTTTGGAAAC ATTCCTGTGG AAAGCCACTC
    TTTTCTTCTC CACGGTGTTG CCTACAGTAT GTTTTTATTG GCTGTGTAGA TGGAAATTTA
    CTCTGCTTTA CTCACTTTGG AGAACAGGTT TGGCAATTCT CTACCAATGA ACCAATCTTT
    TCATCCCCAT GTACCTCAGC ATCAGAGCAA GAAATATTTT TTGGTTCTCA TGACTGCTTT
    ATCTACTGTT GTAGTATGAA AGGTCACCTC CAGTGGAAAT TTGAAACTAC TTCAAGAGTG
    TATTCAACGC CTTTTGTTTT CCGTAACCAC AACCATAACA GTGAAATGTT ACTGGCAGCG
    GCATCTACTG ATGGGAAACT GTGGATCTTG GAATCTAAGA GTGGACAGTT GCAAAGTGTG
    TATGAACTTC CTGGAGAAGT TTTCTCTTCT CCTGTGGTCT GGGAATCAAT GCTTATTATT
    GGATGTAGAA ATAATTATGT TTATTGTCTG GATGTATTGG GTGACAAAGG TAATGAAGTG
    CAGTCCTTAG AATATACATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90089
    Clone ID Related Accession (Same CDS sequence) XM_015463763.1 , XM_015471626.1
    Accession Version XM_015463763.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3435bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product acyl-CoA synthetase family member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTA AATTCAAGTC TTCCTATGAA GCATTATTGA ACTATGATAT GCTTAAAGTA
    GAACATAATG ACCTAGTACT CTTCAGACTT CACTGGAAAA ATGTCGAGGT GAGCCTGGTG
    CTAAATGATA GAAAAGAGAA ATATGAAAAA GGAAAGATGC CAAAGAGTAT GCATTCTGAG
    AGCAGCAGTG AAGAAAAGTC AGAAGAGCAC ATGGATATGA GGCAAAAGCA CTGCTTGGCC
    TATGTTCTCC ATACGTCAGG CACTACAGGG ATACCTAAGA TTGTCAGAGT CCCTCATGCA
    TGTATAGTGC CAAACATCCA GCATTTTCGG GTGCTTTTTG AAATCACTCA TGAGGATGTT
    TTGTTTCTGG CTTCACCTCT GACCTTTGAT CCTTCTGTGG TGGAAATATT TGTTGCTTTG
    TCAAGTGGTG CCTCTTTGCT TATTGTGCCA ACTTCTGTCA AAATGGTCCC ATCAAAATTA
    GCTGCTGTTC TGTTTTCCCA TCACAGAGTG ACTATTTTAC AGGCAACGCC AACATTGCTT
    AGAAGATTTG GATCTCAGCT CATCAAGTCA ACTGTTTTAT CCGCCAGTAC CTCTCTTCGC
    ATATTGGCCC TTGGTGGTGA AGCATTTCCA TCATTAACTG TTCTCAAAAG CTGGAGAGGA
    GTGGGCAATA AAACACAAAT ATTTAATATT TATGGCATCA CAGAGGTATC AAGTTGGGCG
    ACTTTCTACA GGATTCCAGA GAAGATTCTT AACTCTACTT TGAAGTACGG GTTTCTGTTA
    CTACGAAGAG TAGAGAGAGG ATGTGAATTG CCTGTACAAC TGGGATTCCC ACTGCTTGGA
    ACAGTAGTTG AAGTCAGAGA TACTAACGGT TTCACAATTC AAGAAGGCAA TGGCCAAGTA
    TTTTTAGGTG GCAGAAATAG AGTATGTTTT CTCGATGGCG AAGTGACGGT ACCACTTGGC
    ACAATGAGAG CCACAGGAGA CTTTGTGACT ATAAAAGATG GAGAGATATT TTTCTTGGGA
    CGAAAGGACA GTCAGATCAA ACGTCATGGC AAACGTCTTA ACATAGAACT TGTACAACAG
    ATTGCTGAAG GTCTTCAACA AGTGGAGTCT TGTGCAGTTA CATGGTATAA CCAGGAAAAA
    TTGATTTTGT TCATGGTGAC TAAAAATGAT TTAGTAAAGG ATCACATCTT TGAAGAACTG
    CAGAAACATC TTCCAAGTCA TGCCATCCCA GATGAGCTTG TGTTAATTAA TTCTCTGCCA
    TTTACATCTC ATGGCAAAAT TGATGTTTCT GAGTTAAATA AGATATATTT AAACTACAGA
    GACTTGAAGT CTGAGTGTAA GCTCAGTGGA AAAGAGGAAC TTTGGGAAAA ATTACACCAT
    TTGTGGAAGT CTGTTCTGAG TCTCTCAGAA GATGCTTTGA AGATTCCTGA TGAGTCACTC
    TTCTTAAATA GTGGTGGAGA TTCCTTGAAA TCCATACGGC TTCTCAATGA GATTGAAAAC
    CTTGTTGGCA CATCAGTACC TGGGCTTCTG GAAATTATTC TCAGCAGTTC TATTTTACGG
    ATTTACAATC ACATCCTTCA AACAGTGTTT TCAGATAAAG ATATGACACT TAGCAAGAAT
    CATGCCATGA AAAGAAAATT CAACGTTACT CAAGAGGAAA CCAGTGGAAA ATCTTTACAT
    CAGGAAACTG TCATGCCTTT AAATTGTGAC AAGGAGATCG ATGCTTTTAT TGCACTGAGC
    AGAGGGAATC AGATTTTGTC TTTGAATACT GATAAGTATT TAACTAAGTT GGGACATTGC
    CCTTCAACCT ATTCTTCTGA TTTAAATTCA CAGACTGACA TTCAAAATAG AGAAAGCCTA
    AATACTCCAC CTCTTATTGG GAAGTCAGAA GATCCATCCT GTGTTGTGGA AGTTTCTGAA
    GAGGAAACAC CTGTGATTAG GGCTGAGAAA ATGGAGTTTC GTGTGAGGTG GAGGTCAGAC
    ACAGGCAAAT GTGTGGATGC TTCACCTCTG GTTGTAATAC CAGCTATTGA TAAGTCATCT
    GCAACTGTGT ACATTGGCTC TCATTCTCAT AGAATGATAG CAGTTGATCT TTACTCTGGA
    AAGGTGAAAT GGGAACAGAT TTTAGGAGAT CGCATTGAAT CCTCAGCATG TGTATCTAAG
    TGTGGAAACT TTATTGTAGT GGGCTGTTAT AATGGGTTAG TTTATGTTCT GAAAAGTAAT
    AGTGGAGAAA AATACTGGAT GTTTAGTACT GAGGATGCTG TCAAAAGCTC AGCAACCATG
    GATCCAACCA CAGGACTTCT TTACATTGGA TCTCATGACC AGCATGCATA CGCTTTGGAT
    ATATATAAGA AGAAGTGTGT TTGGAAATTG AAATGTGGAG GAACTGTTTT TTCTTCTCCT
    TGTTTGAGCC TGATTCCATC TCACTTGTAT TTTGCTACAC TGGGAGGACT TTTACTGGCT
    ATAAATCCTG CCACTGGGGA CAGACTTTGG AAACATTCCT GTGGAAAGCC ACTCTTTTCT
    TCTCCACGGT GTTGCCTACA GTATGTTTTT ATTGGCTGTG TAGATGGAAA TTTACTCTGC
    TTTACTCACT TTGGAGAACA GGTTTGGCAA TTCTCTACCA ATGAACCAAT CTTTTCATCC
    CCATGTACCT CAGCATCAGA GCAAGAAATA TTTTTTGGTT CTCATGACTG CTTTATCTAC
    TGTTGTAGTA TGAAAGGTCA CCTCCAGTGG AAATTTGAAA CTACTTCAAG AGTGTATTCA
    ACGCCTTTTG TTTTCCGTAA CCACAACCAT AACAGTGAAA TGTTACTGGC AGCGGCATCT
    ACTGATGGGA AACTGTGGAT CTTGGAATCT AAGAGTGGAC AGTTGCAAAG TGTGTATGAA
    CTTCCTGGAG AAGTTTTCTC TTCTCCTGTG GTCTGGGAAT CAATGCTTAT TATTGGATGT
    AGAAATAATT ATGTTTATTG TCTGGATGTA TTGGGTGACA AAGGTAATGA AGTGCAGTCC
    TTAGAATATA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319249.1
    CDS438..3872
    Translation

    Target ORF information:

    RefSeq Version XM_015463763.1
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463763.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTA AATTCAAGTC TTCCTATGAA GCATTATTGA ACTATGATAT GCTTAAAGTA
    GAACATAATG ACCTAGTACT CTTCAGACTT CACTGGAAAA ATGTCGAGGT GAGCCTGGTG
    CTAAATGATA GAAAAGAGAA ATATGAAAAA GGAAAGATGC CAAAGAGTAT GCATTCTGAG
    AGCAGCAGTG AAGAAAAGTC AGAAGAGCAC ATGGATATGA GGCAAAAGCA CTGCTTGGCC
    TATGTTCTCC ATACGTCAGG CACTACAGGG ATACCTAAGA TTGTCAGAGT CCCTCATGCA
    TGTATAGTGC CAAACATCCA GCATTTTCGG GTGCTTTTTG AAATCACTCA TGAGGATGTT
    TTGTTTCTGG CTTCACCTCT GACCTTTGAT CCTTCTGTGG TGGAAATATT TGTTGCTTTG
    TCAAGTGGTG CCTCTTTGCT TATTGTGCCA ACTTCTGTCA AAATGGTCCC ATCAAAATTA
    GCTGCTGTTC TGTTTTCCCA TCACAGAGTG ACTATTTTAC AGGCAACGCC AACATTGCTT
    AGAAGATTTG GATCTCAGCT CATCAAGTCA ACTGTTTTAT CCGCCAGTAC CTCTCTTCGC
    ATATTGGCCC TTGGTGGTGA AGCATTTCCA TCATTAACTG TTCTCAAAAG CTGGAGAGGA
    GTGGGCAATA AAACACAAAT ATTTAATATT TATGGCATCA CAGAGGTATC AAGTTGGGCG
    ACTTTCTACA GGATTCCAGA GAAGATTCTT AACTCTACTT TGAAGTACGG GTTTCTGTTA
    CTACGAAGAG TAGAGAGAGG ATGTGAATTG CCTGTACAAC TGGGATTCCC ACTGCTTGGA
    ACAGTAGTTG AAGTCAGAGA TACTAACGGT TTCACAATTC AAGAAGGCAA TGGCCAAGTA
    TTTTTAGGTG GCAGAAATAG AGTATGTTTT CTCGATGGCG AAGTGACGGT ACCACTTGGC
    ACAATGAGAG CCACAGGAGA CTTTGTGACT ATAAAAGATG GAGAGATATT TTTCTTGGGA
    CGAAAGGACA GTCAGATCAA ACGTCATGGC AAACGTCTTA ACATAGAACT TGTACAACAG
    ATTGCTGAAG GTCTTCAACA AGTGGAGTCT TGTGCAGTTA CATGGTATAA CCAGGAAAAA
    TTGATTTTGT TCATGGTGAC TAAAAATGAT TTAGTAAAGG ATCACATCTT TGAAGAACTG
    CAGAAACATC TTCCAAGTCA TGCCATCCCA GATGAGCTTG TGTTAATTAA TTCTCTGCCA
    TTTACATCTC ATGGCAAAAT TGATGTTTCT GAGTTAAATA AGATATATTT AAACTACAGA
    GACTTGAAGT CTGAGTGTAA GCTCAGTGGA AAAGAGGAAC TTTGGGAAAA ATTACACCAT
    TTGTGGAAGT CTGTTCTGAG TCTCTCAGAA GATGCTTTGA AGATTCCTGA TGAGTCACTC
    TTCTTAAATA GTGGTGGAGA TTCCTTGAAA TCCATACGGC TTCTCAATGA GATTGAAAAC
    CTTGTTGGCA CATCAGTACC TGGGCTTCTG GAAATTATTC TCAGCAGTTC TATTTTACGG
    ATTTACAATC ACATCCTTCA AACAGTGTTT TCAGATAAAG ATATGACACT TAGCAAGAAT
    CATGCCATGA AAAGAAAATT CAACGTTACT CAAGAGGAAA CCAGTGGAAA ATCTTTACAT
    CAGGAAACTG TCATGCCTTT AAATTGTGAC AAGGAGATCG ATGCTTTTAT TGCACTGAGC
    AGAGGGAATC AGATTTTGTC TTTGAATACT GATAAGTATT TAACTAAGTT GGGACATTGC
    CCTTCAACCT ATTCTTCTGA TTTAAATTCA CAGACTGACA TTCAAAATAG AGAAAGCCTA
    AATACTCCAC CTCTTATTGG GAAGTCAGAA GATCCATCCT GTGTTGTGGA AGTTTCTGAA
    GAGGAAACAC CTGTGATTAG GGCTGAGAAA ATGGAGTTTC GTGTGAGGTG GAGGTCAGAC
    ACAGGCAAAT GTGTGGATGC TTCACCTCTG GTTGTAATAC CAGCTATTGA TAAGTCATCT
    GCAACTGTGT ACATTGGCTC TCATTCTCAT AGAATGATAG CAGTTGATCT TTACTCTGGA
    AAGGTGAAAT GGGAACAGAT TTTAGGAGAT CGCATTGAAT CCTCAGCATG TGTATCTAAG
    TGTGGAAACT TTATTGTAGT GGGCTGTTAT AATGGGTTAG TTTATGTTCT GAAAAGTAAT
    AGTGGAGAAA AATACTGGAT GTTTAGTACT GAGGATGCTG TCAAAAGCTC AGCAACCATG
    GATCCAACCA CAGGACTTCT TTACATTGGA TCTCATGACC AGCATGCATA CGCTTTGGAT
    ATATATAAGA AGAAGTGTGT TTGGAAATTG AAATGTGGAG GAACTGTTTT TTCTTCTCCT
    TGTTTGAGCC TGATTCCATC TCACTTGTAT TTTGCTACAC TGGGAGGACT TTTACTGGCT
    ATAAATCCTG CCACTGGGGA CAGACTTTGG AAACATTCCT GTGGAAAGCC ACTCTTTTCT
    TCTCCACGGT GTTGCCTACA GTATGTTTTT ATTGGCTGTG TAGATGGAAA TTTACTCTGC
    TTTACTCACT TTGGAGAACA GGTTTGGCAA TTCTCTACCA ATGAACCAAT CTTTTCATCC
    CCATGTACCT CAGCATCAGA GCAAGAAATA TTTTTTGGTT CTCATGACTG CTTTATCTAC
    TGTTGTAGTA TGAAAGGTCA CCTCCAGTGG AAATTTGAAA CTACTTCAAG AGTGTATTCA
    ACGCCTTTTG TTTTCCGTAA CCACAACCAT AACAGTGAAA TGTTACTGGC AGCGGCATCT
    ACTGATGGGA AACTGTGGAT CTTGGAATCT AAGAGTGGAC AGTTGCAAAG TGTGTATGAA
    CTTCCTGGAG AAGTTTTCTC TTCTCCTGTG GTCTGGGAAT CAATGCTTAT TATTGGATGT
    AGAAATAATT ATGTTTATTG TCTGGATGTA TTGGGTGACA AAGGTAATGA AGTGCAGTCC
    TTAGAATATA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90089
    Clone ID Related Accession (Same CDS sequence) XM_015463763.1 , XM_015471626.1
    Accession Version XM_015471626.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3435bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product acyl-CoA synthetase family member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103975.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTA AATTCAAGTC TTCCTATGAA GCATTATTGA ACTATGATAT GCTTAAAGTA
    GAACATAATG ACCTAGTACT CTTCAGACTT CACTGGAAAA ATGTCGAGGT GAGCCTGGTG
    CTAAATGATA GAAAAGAGAA ATATGAAAAA GGAAAGATGC CAAAGAGTAT GCATTCTGAG
    AGCAGCAGTG AAGAAAAGTC AGAAGAGCAC ATGGATATGA GGCAAAAGCA CTGCTTGGCC
    TATGTTCTCC ATACGTCAGG CACTACAGGG ATACCTAAGA TTGTCAGAGT CCCTCATGCA
    TGTATAGTGC CAAACATCCA GCATTTTCGG GTGCTTTTTG AAATCACTCA TGAGGATGTT
    TTGTTTCTGG CTTCACCTCT GACCTTTGAT CCTTCTGTGG TGGAAATATT TGTTGCTTTG
    TCAAGTGGTG CCTCTTTGCT TATTGTGCCA ACTTCTGTCA AAATGGTCCC ATCAAAATTA
    GCTGCTGTTC TGTTTTCCCA TCACAGAGTG ACTATTTTAC AGGCAACGCC AACATTGCTT
    AGAAGATTTG GATCTCAGCT CATCAAGTCA ACTGTTTTAT CCGCCAGTAC CTCTCTTCGC
    ATATTGGCCC TTGGTGGTGA AGCATTTCCA TCATTAACTG TTCTCAAAAG CTGGAGAGGA
    GTGGGCAATA AAACACAAAT ATTTAATATT TATGGCATCA CAGAGGTATC AAGTTGGGCG
    ACTTTCTACA GGATTCCAGA GAAGATTCTT AACTCTACTT TGAAGTACGG GTTTCTGTTA
    CTACGAAGAG TAGAGAGAGG ATGTGAATTG CCTGTACAAC TGGGATTCCC ACTGCTTGGA
    ACAGTAGTTG AAGTCAGAGA TACTAACGGT TTCACAATTC AAGAAGGCAA TGGCCAAGTA
    TTTTTAGGTG GCAGAAATAG AGTATGTTTT CTCGATGGCG AAGTGACGGT ACCACTTGGC
    ACAATGAGAG CCACAGGAGA CTTTGTGACT ATAAAAGATG GAGAGATATT TTTCTTGGGA
    CGAAAGGACA GTCAGATCAA ACGTCATGGC AAACGTCTTA ACATAGAACT TGTACAACAG
    ATTGCTGAAG GTCTTCAACA AGTGGAGTCT TGTGCAGTTA CATGGTATAA CCAGGAAAAA
    TTGATTTTGT TCATGGTGAC TAAAAATGAT TTAGTAAAGG ATCACATCTT TGAAGAACTG
    CAGAAACATC TTCCAAGTCA TGCCATCCCA GATGAGCTTG TGTTAATTAA TTCTCTGCCA
    TTTACATCTC ATGGCAAAAT TGATGTTTCT GAGTTAAATA AGATATATTT AAACTACAGA
    GACTTGAAGT CTGAGTGTAA GCTCAGTGGA AAAGAGGAAC TTTGGGAAAA ATTACACCAT
    TTGTGGAAGT CTGTTCTGAG TCTCTCAGAA GATGCTTTGA AGATTCCTGA TGAGTCACTC
    TTCTTAAATA GTGGTGGAGA TTCCTTGAAA TCCATACGGC TTCTCAATGA GATTGAAAAC
    CTTGTTGGCA CATCAGTACC TGGGCTTCTG GAAATTATTC TCAGCAGTTC TATTTTACGG
    ATTTACAATC ACATCCTTCA AACAGTGTTT TCAGATAAAG ATATGACACT TAGCAAGAAT
    CATGCCATGA AAAGAAAATT CAACGTTACT CAAGAGGAAA CCAGTGGAAA ATCTTTACAT
    CAGGAAACTG TCATGCCTTT AAATTGTGAC AAGGAGATCG ATGCTTTTAT TGCACTGAGC
    AGAGGGAATC AGATTTTGTC TTTGAATACT GATAAGTATT TAACTAAGTT GGGACATTGC
    CCTTCAACCT ATTCTTCTGA TTTAAATTCA CAGACTGACA TTCAAAATAG AGAAAGCCTA
    AATACTCCAC CTCTTATTGG GAAGTCAGAA GATCCATCCT GTGTTGTGGA AGTTTCTGAA
    GAGGAAACAC CTGTGATTAG GGCTGAGAAA ATGGAGTTTC GTGTGAGGTG GAGGTCAGAC
    ACAGGCAAAT GTGTGGATGC TTCACCTCTG GTTGTAATAC CAGCTATTGA TAAGTCATCT
    GCAACTGTGT ACATTGGCTC TCATTCTCAT AGAATGATAG CAGTTGATCT TTACTCTGGA
    AAGGTGAAAT GGGAACAGAT TTTAGGAGAT CGCATTGAAT CCTCAGCATG TGTATCTAAG
    TGTGGAAACT TTATTGTAGT GGGCTGTTAT AATGGGTTAG TTTATGTTCT GAAAAGTAAT
    AGTGGAGAAA AATACTGGAT GTTTAGTACT GAGGATGCTG TCAAAAGCTC AGCAACCATG
    GATCCAACCA CAGGACTTCT TTACATTGGA TCTCATGACC AGCATGCATA CGCTTTGGAT
    ATATATAAGA AGAAGTGTGT TTGGAAATTG AAATGTGGAG GAACTGTTTT TTCTTCTCCT
    TGTTTGAGCC TGATTCCATC TCACTTGTAT TTTGCTACAC TGGGAGGACT TTTACTGGCT
    ATAAATCCTG CCACTGGGGA CAGACTTTGG AAACATTCCT GTGGAAAGCC ACTCTTTTCT
    TCTCCACGGT GTTGCCTACA GTATGTTTTT ATTGGCTGTG TAGATGGAAA TTTACTCTGC
    TTTACTCACT TTGGAGAACA GGTTTGGCAA TTCTCTACCA ATGAACCAAT CTTTTCATCC
    CCATGTACCT CAGCATCAGA GCAAGAAATA TTTTTTGGTT CTCATGACTG CTTTATCTAC
    TGTTGTAGTA TGAAAGGTCA CCTCCAGTGG AAATTTGAAA CTACTTCAAG AGTGTATTCA
    ACGCCTTTTG TTTTCCGTAA CCACAACCAT AACAGTGAAA TGTTACTGGC AGCGGCATCT
    ACTGATGGGA AACTGTGGAT CTTGGAATCT AAGAGTGGAC AGTTGCAAAG TGTGTATGAA
    CTTCCTGGAG AAGTTTTCTC TTCTCCTGTG GTCTGGGAAT CAATGCTTAT TATTGGATGT
    AGAAATAATT ATGTTTATTG TCTGGATGTA TTGGGTGACA AAGGTAATGA AGTGCAGTCC
    TTAGAATATA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327112.1
    CDS438..3872
    Translation

    Target ORF information:

    RefSeq Version XM_015471626.1
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471626.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTA AATTCAAGTC TTCCTATGAA GCATTATTGA ACTATGATAT GCTTAAAGTA
    GAACATAATG ACCTAGTACT CTTCAGACTT CACTGGAAAA ATGTCGAGGT GAGCCTGGTG
    CTAAATGATA GAAAAGAGAA ATATGAAAAA GGAAAGATGC CAAAGAGTAT GCATTCTGAG
    AGCAGCAGTG AAGAAAAGTC AGAAGAGCAC ATGGATATGA GGCAAAAGCA CTGCTTGGCC
    TATGTTCTCC ATACGTCAGG CACTACAGGG ATACCTAAGA TTGTCAGAGT CCCTCATGCA
    TGTATAGTGC CAAACATCCA GCATTTTCGG GTGCTTTTTG AAATCACTCA TGAGGATGTT
    TTGTTTCTGG CTTCACCTCT GACCTTTGAT CCTTCTGTGG TGGAAATATT TGTTGCTTTG
    TCAAGTGGTG CCTCTTTGCT TATTGTGCCA ACTTCTGTCA AAATGGTCCC ATCAAAATTA
    GCTGCTGTTC TGTTTTCCCA TCACAGAGTG ACTATTTTAC AGGCAACGCC AACATTGCTT
    AGAAGATTTG GATCTCAGCT CATCAAGTCA ACTGTTTTAT CCGCCAGTAC CTCTCTTCGC
    ATATTGGCCC TTGGTGGTGA AGCATTTCCA TCATTAACTG TTCTCAAAAG CTGGAGAGGA
    GTGGGCAATA AAACACAAAT ATTTAATATT TATGGCATCA CAGAGGTATC AAGTTGGGCG
    ACTTTCTACA GGATTCCAGA GAAGATTCTT AACTCTACTT TGAAGTACGG GTTTCTGTTA
    CTACGAAGAG TAGAGAGAGG ATGTGAATTG CCTGTACAAC TGGGATTCCC ACTGCTTGGA
    ACAGTAGTTG AAGTCAGAGA TACTAACGGT TTCACAATTC AAGAAGGCAA TGGCCAAGTA
    TTTTTAGGTG GCAGAAATAG AGTATGTTTT CTCGATGGCG AAGTGACGGT ACCACTTGGC
    ACAATGAGAG CCACAGGAGA CTTTGTGACT ATAAAAGATG GAGAGATATT TTTCTTGGGA
    CGAAAGGACA GTCAGATCAA ACGTCATGGC AAACGTCTTA ACATAGAACT TGTACAACAG
    ATTGCTGAAG GTCTTCAACA AGTGGAGTCT TGTGCAGTTA CATGGTATAA CCAGGAAAAA
    TTGATTTTGT TCATGGTGAC TAAAAATGAT TTAGTAAAGG ATCACATCTT TGAAGAACTG
    CAGAAACATC TTCCAAGTCA TGCCATCCCA GATGAGCTTG TGTTAATTAA TTCTCTGCCA
    TTTACATCTC ATGGCAAAAT TGATGTTTCT GAGTTAAATA AGATATATTT AAACTACAGA
    GACTTGAAGT CTGAGTGTAA GCTCAGTGGA AAAGAGGAAC TTTGGGAAAA ATTACACCAT
    TTGTGGAAGT CTGTTCTGAG TCTCTCAGAA GATGCTTTGA AGATTCCTGA TGAGTCACTC
    TTCTTAAATA GTGGTGGAGA TTCCTTGAAA TCCATACGGC TTCTCAATGA GATTGAAAAC
    CTTGTTGGCA CATCAGTACC TGGGCTTCTG GAAATTATTC TCAGCAGTTC TATTTTACGG
    ATTTACAATC ACATCCTTCA AACAGTGTTT TCAGATAAAG ATATGACACT TAGCAAGAAT
    CATGCCATGA AAAGAAAATT CAACGTTACT CAAGAGGAAA CCAGTGGAAA ATCTTTACAT
    CAGGAAACTG TCATGCCTTT AAATTGTGAC AAGGAGATCG ATGCTTTTAT TGCACTGAGC
    AGAGGGAATC AGATTTTGTC TTTGAATACT GATAAGTATT TAACTAAGTT GGGACATTGC
    CCTTCAACCT ATTCTTCTGA TTTAAATTCA CAGACTGACA TTCAAAATAG AGAAAGCCTA
    AATACTCCAC CTCTTATTGG GAAGTCAGAA GATCCATCCT GTGTTGTGGA AGTTTCTGAA
    GAGGAAACAC CTGTGATTAG GGCTGAGAAA ATGGAGTTTC GTGTGAGGTG GAGGTCAGAC
    ACAGGCAAAT GTGTGGATGC TTCACCTCTG GTTGTAATAC CAGCTATTGA TAAGTCATCT
    GCAACTGTGT ACATTGGCTC TCATTCTCAT AGAATGATAG CAGTTGATCT TTACTCTGGA
    AAGGTGAAAT GGGAACAGAT TTTAGGAGAT CGCATTGAAT CCTCAGCATG TGTATCTAAG
    TGTGGAAACT TTATTGTAGT GGGCTGTTAT AATGGGTTAG TTTATGTTCT GAAAAGTAAT
    AGTGGAGAAA AATACTGGAT GTTTAGTACT GAGGATGCTG TCAAAAGCTC AGCAACCATG
    GATCCAACCA CAGGACTTCT TTACATTGGA TCTCATGACC AGCATGCATA CGCTTTGGAT
    ATATATAAGA AGAAGTGTGT TTGGAAATTG AAATGTGGAG GAACTGTTTT TTCTTCTCCT
    TGTTTGAGCC TGATTCCATC TCACTTGTAT TTTGCTACAC TGGGAGGACT TTTACTGGCT
    ATAAATCCTG CCACTGGGGA CAGACTTTGG AAACATTCCT GTGGAAAGCC ACTCTTTTCT
    TCTCCACGGT GTTGCCTACA GTATGTTTTT ATTGGCTGTG TAGATGGAAA TTTACTCTGC
    TTTACTCACT TTGGAGAACA GGTTTGGCAA TTCTCTACCA ATGAACCAAT CTTTTCATCC
    CCATGTACCT CAGCATCAGA GCAAGAAATA TTTTTTGGTT CTCATGACTG CTTTATCTAC
    TGTTGTAGTA TGAAAGGTCA CCTCCAGTGG AAATTTGAAA CTACTTCAAG AGTGTATTCA
    ACGCCTTTTG TTTTCCGTAA CCACAACCAT AACAGTGAAA TGTTACTGGC AGCGGCATCT
    ACTGATGGGA AACTGTGGAT CTTGGAATCT AAGAGTGGAC AGTTGCAAAG TGTGTATGAA
    CTTCCTGGAG AAGTTTTCTC TTCTCCTGTG GTCTGGGAAT CAATGCTTAT TATTGGATGT
    AGAAATAATT ATGTTTATTG TCTGGATGTA TTGGGTGACA AAGGTAATGA AGTGCAGTCC
    TTAGAATATA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90090
    Clone ID Related Accession (Same CDS sequence) XM_015471627.1 , XM_015463764.1
    Accession Version XM_015471627.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3312bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product acyl-CoA synthetase family member 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103975.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTA AATTCAAGTC TTCCTATGAA GCATTATTGA ACTATGATAT GCTTAAAGTA
    GAACATAATG ACCTAGTACT CTTCAGACTT CACTGGAAAA ATGTCGAGGT GAGCCTGGTG
    CTAAATGATA GAAAAGAGAA ATATGAAAAA GGAAAGATGC CAAAGAGTAT GCATTCTGAG
    AGCAGCAGTG AAGAAAAGTC AGAAGAGCAC ATGGATATGA GGCAAAAGCA CTGCTTGGCC
    TATGTTCTCC ATACGTCAGG CACTACAGGG ATACCTAAGA TTGTCAGAGT CCCTCATGCA
    TGTATAGTGC CAAACATCCA GCATTTTCGG GTGCTTTTTG AAATCACTCA TGAGGATGTT
    TTGTTTCTGG CTTCACCTCT GACCTTTGAT CCTTCTGTGG TGGAAATATT TGTTGCTTTG
    TCAAGTGGTG CCTCTTTGCT TATTGTGCCA ACTTCTGTCA AAATGGTCCC ATCAAAATTA
    GCTGCTGTTC TGTTTTCCCA TCACAGAGTG ACTATTTTAC AGGCAACGCC AACATTGCTT
    AGAAGATTTG GATCTCAGCT CATCAAGTCA ACTGTTTTAT CCGCCAGTAC CTCTCTTCGC
    ATATTGGCCC TTGGTGGTGA AGCATTTCCA TCATTAACTG TTCTCAAAAG CTGGAGAGGA
    GTGGGCAATA AAACACAAAT ATTTAATATT TATGGCATCA CAGAGGTATC AAGTTGGGCG
    ACTTTCTACA GGATTCCAGA GAAGATTCTT AACTCTACTT TGAAGTACGG GTTTCTGTTA
    CTACGAAGAG TAGAGAGAGG ATGTGAATTG CCTGTACAAC TGGGATTCCC ACTGCTTGGA
    ACAGTAGTTG AAGTCAGAGA TACTAACGGT TTCACAATTC AAGAAGGCAA TGGCCAAGTA
    TTTTTAGGTG GCAGAAATAG AGTATGTTTT CTCGATGGCG AAGTGACGGT ACCACTTGGC
    ACAATGAGAG CCACAGGAGA CTTTGTGACT ATAAAAGATG GAGAGATATT TTTCTTGGGA
    CGAAAGGACA GTCAGATCAA ACGTCATGGC AAACGTCTTA ACATAGAACT TGTACAACAG
    ATTGCTGAAG GTCTTCAACA AGTGGAGTCT TGTGCAGTTA CATGGTATAA CCAGGAAAAA
    TTGATTTTGT TCATGGTGAC TAAAAATGAT TTAGTAAAGG ATCACATCTT TGAAGAACTG
    CAGAAACATC TTCCAAGTCA TGCCATCCCA GATGAGCTTG TGTTAATTAA TTCTCTGCCA
    TTTACATCTC ATGGCAAAAT TGATGTTTCT GAGTTAAATA AGATATATTT AAACTACAGA
    GACTTGAAGT CTGAGTGTAA GCTCAGTGGA AAAGAGGAAC TTTGGGAAAA ATTACACCAT
    TTGTGGAAGT CTGTTCTGAG TCTCTCAGAA GATGCTTTGA AGATTCCTGA TGAGTCACTC
    TTCTTAAATA GTGGTGGAGA TTCCTTGAAA TCCATACGGC TTCTCAATGA GATTGAAAAC
    CTTGTTGGCA CATCAGTACC TGGGCTTCTG GAAATTATTC TCAGCAGTTC TATTTTACGG
    ATTTACAATC ACATCCTTCA AACAGTGTTT TCAGATAAAG ATATGACACT TAGCAAGAAT
    CATGCCATGA AAAGAAAATT CAACGTTACT CAAGAGGAAA CCAGTGGAAA ATCTTTACAT
    CAGGAAACTG TCATGCCTTT AAATTGTGAC AAGGAGATCG ATGCTTTTAT TGCACTGAGC
    AGAGGGAATC AGATTTTGTC TTTGAATACT GATAAGTATT TAACTAAGTT GGGACATTGC
    CCTTCAACCT ATTCTTCTGA TTTAAATTCA CAGACTGACA TTCAAAATAG AGAAAGCCTA
    AATACTCCAC CTCTTATTGG GAAGTCAGAA GATCCATCCT GTGTTGTGGA AGTTTCTGAA
    GAGGAAACAC CTGTGATTAG GGCTGAGAAA ATGGAGTTTC GTGTGAGGTG GAGGTCAGAC
    ACAGGCAAAT GTGTGGATGC TTCACCTCTG GTTGTAATAC CAGCTATTGA TAAGTCATCT
    GCAACTGTGT ACATTGGCTC TCATTCTCAT AGAATGATAG CAGTTGATCT TTACTCTGGA
    AAGGTGAAAT GGGAACAGAT TTTAGGAGAT CGCATTGAAT CCTCAGCATG TGTATCTAAG
    TGTGGAAACT TTATTGTAGT GGGCTGTTAT AATGGGTTAG TTTATGTTCT GAAAAGTAAT
    AGTGGAGAAA AATACTGGAT GTTTAGTACT GAGGATGCTG TCAAAAGCTC AGCAACCATG
    GATCCAACCA CAGGACTTCT TTACATTGGA TCTCATGACC AGCATGCATA CGCTTTGGAT
    ATATATGCCA CTGGGGACAG ACTTTGGAAA CATTCCTGTG GAAAGCCACT CTTTTCTTCT
    CCACGGTGTT GCCTACAGTA TGTTTTTATT GGCTGTGTAG ATGGAAATTT ACTCTGCTTT
    ACTCACTTTG GAGAACAGGT TTGGCAATTC TCTACCAATG AACCAATCTT TTCATCCCCA
    TGTACCTCAG CATCAGAGCA AGAAATATTT TTTGGTTCTC ATGACTGCTT TATCTACTGT
    TGTAGTATGA AAGGTCACCT CCAGTGGAAA TTTGAAACTA CTTCAAGAGT GTATTCAACG
    CCTTTTGTTT TCCGTAACCA CAACCATAAC AGTGAAATGT TACTGGCAGC GGCATCTACT
    GATGGGAAAC TGTGGATCTT GGAATCTAAG AGTGGACAGT TGCAAAGTGT GTATGAACTT
    CCTGGAGAAG TTTTCTCTTC TCCTGTGGTC TGGGAATCAA TGCTTATTAT TGGATGTAGA
    AATAATTATG TTTATTGTCT GGATGTATTG GGTGACAAAG GTAATGAAGT GCAGTCCTTA
    GAATATACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327113.1
    CDS438..3749
    Translation

    Target ORF information:

    RefSeq Version XM_015471627.1
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471627.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTA AATTCAAGTC TTCCTATGAA GCATTATTGA ACTATGATAT GCTTAAAGTA
    GAACATAATG ACCTAGTACT CTTCAGACTT CACTGGAAAA ATGTCGAGGT GAGCCTGGTG
    CTAAATGATA GAAAAGAGAA ATATGAAAAA GGAAAGATGC CAAAGAGTAT GCATTCTGAG
    AGCAGCAGTG AAGAAAAGTC AGAAGAGCAC ATGGATATGA GGCAAAAGCA CTGCTTGGCC
    TATGTTCTCC ATACGTCAGG CACTACAGGG ATACCTAAGA TTGTCAGAGT CCCTCATGCA
    TGTATAGTGC CAAACATCCA GCATTTTCGG GTGCTTTTTG AAATCACTCA TGAGGATGTT
    TTGTTTCTGG CTTCACCTCT GACCTTTGAT CCTTCTGTGG TGGAAATATT TGTTGCTTTG
    TCAAGTGGTG CCTCTTTGCT TATTGTGCCA ACTTCTGTCA AAATGGTCCC ATCAAAATTA
    GCTGCTGTTC TGTTTTCCCA TCACAGAGTG ACTATTTTAC AGGCAACGCC AACATTGCTT
    AGAAGATTTG GATCTCAGCT CATCAAGTCA ACTGTTTTAT CCGCCAGTAC CTCTCTTCGC
    ATATTGGCCC TTGGTGGTGA AGCATTTCCA TCATTAACTG TTCTCAAAAG CTGGAGAGGA
    GTGGGCAATA AAACACAAAT ATTTAATATT TATGGCATCA CAGAGGTATC AAGTTGGGCG
    ACTTTCTACA GGATTCCAGA GAAGATTCTT AACTCTACTT TGAAGTACGG GTTTCTGTTA
    CTACGAAGAG TAGAGAGAGG ATGTGAATTG CCTGTACAAC TGGGATTCCC ACTGCTTGGA
    ACAGTAGTTG AAGTCAGAGA TACTAACGGT TTCACAATTC AAGAAGGCAA TGGCCAAGTA
    TTTTTAGGTG GCAGAAATAG AGTATGTTTT CTCGATGGCG AAGTGACGGT ACCACTTGGC
    ACAATGAGAG CCACAGGAGA CTTTGTGACT ATAAAAGATG GAGAGATATT TTTCTTGGGA
    CGAAAGGACA GTCAGATCAA ACGTCATGGC AAACGTCTTA ACATAGAACT TGTACAACAG
    ATTGCTGAAG GTCTTCAACA AGTGGAGTCT TGTGCAGTTA CATGGTATAA CCAGGAAAAA
    TTGATTTTGT TCATGGTGAC TAAAAATGAT TTAGTAAAGG ATCACATCTT TGAAGAACTG
    CAGAAACATC TTCCAAGTCA TGCCATCCCA GATGAGCTTG TGTTAATTAA TTCTCTGCCA
    TTTACATCTC ATGGCAAAAT TGATGTTTCT GAGTTAAATA AGATATATTT AAACTACAGA
    GACTTGAAGT CTGAGTGTAA GCTCAGTGGA AAAGAGGAAC TTTGGGAAAA ATTACACCAT
    TTGTGGAAGT CTGTTCTGAG TCTCTCAGAA GATGCTTTGA AGATTCCTGA TGAGTCACTC
    TTCTTAAATA GTGGTGGAGA TTCCTTGAAA TCCATACGGC TTCTCAATGA GATTGAAAAC
    CTTGTTGGCA CATCAGTACC TGGGCTTCTG GAAATTATTC TCAGCAGTTC TATTTTACGG
    ATTTACAATC ACATCCTTCA AACAGTGTTT TCAGATAAAG ATATGACACT TAGCAAGAAT
    CATGCCATGA AAAGAAAATT CAACGTTACT CAAGAGGAAA CCAGTGGAAA ATCTTTACAT
    CAGGAAACTG TCATGCCTTT AAATTGTGAC AAGGAGATCG ATGCTTTTAT TGCACTGAGC
    AGAGGGAATC AGATTTTGTC TTTGAATACT GATAAGTATT TAACTAAGTT GGGACATTGC
    CCTTCAACCT ATTCTTCTGA TTTAAATTCA CAGACTGACA TTCAAAATAG AGAAAGCCTA
    AATACTCCAC CTCTTATTGG GAAGTCAGAA GATCCATCCT GTGTTGTGGA AGTTTCTGAA
    GAGGAAACAC CTGTGATTAG GGCTGAGAAA ATGGAGTTTC GTGTGAGGTG GAGGTCAGAC
    ACAGGCAAAT GTGTGGATGC TTCACCTCTG GTTGTAATAC CAGCTATTGA TAAGTCATCT
    GCAACTGTGT ACATTGGCTC TCATTCTCAT AGAATGATAG CAGTTGATCT TTACTCTGGA
    AAGGTGAAAT GGGAACAGAT TTTAGGAGAT CGCATTGAAT CCTCAGCATG TGTATCTAAG
    TGTGGAAACT TTATTGTAGT GGGCTGTTAT AATGGGTTAG TTTATGTTCT GAAAAGTAAT
    AGTGGAGAAA AATACTGGAT GTTTAGTACT GAGGATGCTG TCAAAAGCTC AGCAACCATG
    GATCCAACCA CAGGACTTCT TTACATTGGA TCTCATGACC AGCATGCATA CGCTTTGGAT
    ATATATGCCA CTGGGGACAG ACTTTGGAAA CATTCCTGTG GAAAGCCACT CTTTTCTTCT
    CCACGGTGTT GCCTACAGTA TGTTTTTATT GGCTGTGTAG ATGGAAATTT ACTCTGCTTT
    ACTCACTTTG GAGAACAGGT TTGGCAATTC TCTACCAATG AACCAATCTT TTCATCCCCA
    TGTACCTCAG CATCAGAGCA AGAAATATTT TTTGGTTCTC ATGACTGCTT TATCTACTGT
    TGTAGTATGA AAGGTCACCT CCAGTGGAAA TTTGAAACTA CTTCAAGAGT GTATTCAACG
    CCTTTTGTTT TCCGTAACCA CAACCATAAC AGTGAAATGT TACTGGCAGC GGCATCTACT
    GATGGGAAAC TGTGGATCTT GGAATCTAAG AGTGGACAGT TGCAAAGTGT GTATGAACTT
    CCTGGAGAAG TTTTCTCTTC TCCTGTGGTC TGGGAATCAA TGCTTATTAT TGGATGTAGA
    AATAATTATG TTTATTGTCT GGATGTATTG GGTGACAAAG GTAATGAAGT GCAGTCCTTA
    GAATATACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90090
    Clone ID Related Accession (Same CDS sequence) XM_015471627.1 , XM_015463764.1
    Accession Version XM_015463764.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3312bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product acyl-CoA synthetase family member 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTA AATTCAAGTC TTCCTATGAA GCATTATTGA ACTATGATAT GCTTAAAGTA
    GAACATAATG ACCTAGTACT CTTCAGACTT CACTGGAAAA ATGTCGAGGT GAGCCTGGTG
    CTAAATGATA GAAAAGAGAA ATATGAAAAA GGAAAGATGC CAAAGAGTAT GCATTCTGAG
    AGCAGCAGTG AAGAAAAGTC AGAAGAGCAC ATGGATATGA GGCAAAAGCA CTGCTTGGCC
    TATGTTCTCC ATACGTCAGG CACTACAGGG ATACCTAAGA TTGTCAGAGT CCCTCATGCA
    TGTATAGTGC CAAACATCCA GCATTTTCGG GTGCTTTTTG AAATCACTCA TGAGGATGTT
    TTGTTTCTGG CTTCACCTCT GACCTTTGAT CCTTCTGTGG TGGAAATATT TGTTGCTTTG
    TCAAGTGGTG CCTCTTTGCT TATTGTGCCA ACTTCTGTCA AAATGGTCCC ATCAAAATTA
    GCTGCTGTTC TGTTTTCCCA TCACAGAGTG ACTATTTTAC AGGCAACGCC AACATTGCTT
    AGAAGATTTG GATCTCAGCT CATCAAGTCA ACTGTTTTAT CCGCCAGTAC CTCTCTTCGC
    ATATTGGCCC TTGGTGGTGA AGCATTTCCA TCATTAACTG TTCTCAAAAG CTGGAGAGGA
    GTGGGCAATA AAACACAAAT ATTTAATATT TATGGCATCA CAGAGGTATC AAGTTGGGCG
    ACTTTCTACA GGATTCCAGA GAAGATTCTT AACTCTACTT TGAAGTACGG GTTTCTGTTA
    CTACGAAGAG TAGAGAGAGG ATGTGAATTG CCTGTACAAC TGGGATTCCC ACTGCTTGGA
    ACAGTAGTTG AAGTCAGAGA TACTAACGGT TTCACAATTC AAGAAGGCAA TGGCCAAGTA
    TTTTTAGGTG GCAGAAATAG AGTATGTTTT CTCGATGGCG AAGTGACGGT ACCACTTGGC
    ACAATGAGAG CCACAGGAGA CTTTGTGACT ATAAAAGATG GAGAGATATT TTTCTTGGGA
    CGAAAGGACA GTCAGATCAA ACGTCATGGC AAACGTCTTA ACATAGAACT TGTACAACAG
    ATTGCTGAAG GTCTTCAACA AGTGGAGTCT TGTGCAGTTA CATGGTATAA CCAGGAAAAA
    TTGATTTTGT TCATGGTGAC TAAAAATGAT TTAGTAAAGG ATCACATCTT TGAAGAACTG
    CAGAAACATC TTCCAAGTCA TGCCATCCCA GATGAGCTTG TGTTAATTAA TTCTCTGCCA
    TTTACATCTC ATGGCAAAAT TGATGTTTCT GAGTTAAATA AGATATATTT AAACTACAGA
    GACTTGAAGT CTGAGTGTAA GCTCAGTGGA AAAGAGGAAC TTTGGGAAAA ATTACACCAT
    TTGTGGAAGT CTGTTCTGAG TCTCTCAGAA GATGCTTTGA AGATTCCTGA TGAGTCACTC
    TTCTTAAATA GTGGTGGAGA TTCCTTGAAA TCCATACGGC TTCTCAATGA GATTGAAAAC
    CTTGTTGGCA CATCAGTACC TGGGCTTCTG GAAATTATTC TCAGCAGTTC TATTTTACGG
    ATTTACAATC ACATCCTTCA AACAGTGTTT TCAGATAAAG ATATGACACT TAGCAAGAAT
    CATGCCATGA AAAGAAAATT CAACGTTACT CAAGAGGAAA CCAGTGGAAA ATCTTTACAT
    CAGGAAACTG TCATGCCTTT AAATTGTGAC AAGGAGATCG ATGCTTTTAT TGCACTGAGC
    AGAGGGAATC AGATTTTGTC TTTGAATACT GATAAGTATT TAACTAAGTT GGGACATTGC
    CCTTCAACCT ATTCTTCTGA TTTAAATTCA CAGACTGACA TTCAAAATAG AGAAAGCCTA
    AATACTCCAC CTCTTATTGG GAAGTCAGAA GATCCATCCT GTGTTGTGGA AGTTTCTGAA
    GAGGAAACAC CTGTGATTAG GGCTGAGAAA ATGGAGTTTC GTGTGAGGTG GAGGTCAGAC
    ACAGGCAAAT GTGTGGATGC TTCACCTCTG GTTGTAATAC CAGCTATTGA TAAGTCATCT
    GCAACTGTGT ACATTGGCTC TCATTCTCAT AGAATGATAG CAGTTGATCT TTACTCTGGA
    AAGGTGAAAT GGGAACAGAT TTTAGGAGAT CGCATTGAAT CCTCAGCATG TGTATCTAAG
    TGTGGAAACT TTATTGTAGT GGGCTGTTAT AATGGGTTAG TTTATGTTCT GAAAAGTAAT
    AGTGGAGAAA AATACTGGAT GTTTAGTACT GAGGATGCTG TCAAAAGCTC AGCAACCATG
    GATCCAACCA CAGGACTTCT TTACATTGGA TCTCATGACC AGCATGCATA CGCTTTGGAT
    ATATATGCCA CTGGGGACAG ACTTTGGAAA CATTCCTGTG GAAAGCCACT CTTTTCTTCT
    CCACGGTGTT GCCTACAGTA TGTTTTTATT GGCTGTGTAG ATGGAAATTT ACTCTGCTTT
    ACTCACTTTG GAGAACAGGT TTGGCAATTC TCTACCAATG AACCAATCTT TTCATCCCCA
    TGTACCTCAG CATCAGAGCA AGAAATATTT TTTGGTTCTC ATGACTGCTT TATCTACTGT
    TGTAGTATGA AAGGTCACCT CCAGTGGAAA TTTGAAACTA CTTCAAGAGT GTATTCAACG
    CCTTTTGTTT TCCGTAACCA CAACCATAAC AGTGAAATGT TACTGGCAGC GGCATCTACT
    GATGGGAAAC TGTGGATCTT GGAATCTAAG AGTGGACAGT TGCAAAGTGT GTATGAACTT
    CCTGGAGAAG TTTTCTCTTC TCCTGTGGTC TGGGAATCAA TGCTTATTAT TGGATGTAGA
    AATAATTATG TTTATTGTCT GGATGTATTG GGTGACAAAG GTAATGAAGT GCAGTCCTTA
    GAATATACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319250.1
    CDS438..3749
    Translation

    Target ORF information:

    RefSeq Version XM_015463764.1
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463764.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTA AATTCAAGTC TTCCTATGAA GCATTATTGA ACTATGATAT GCTTAAAGTA
    GAACATAATG ACCTAGTACT CTTCAGACTT CACTGGAAAA ATGTCGAGGT GAGCCTGGTG
    CTAAATGATA GAAAAGAGAA ATATGAAAAA GGAAAGATGC CAAAGAGTAT GCATTCTGAG
    AGCAGCAGTG AAGAAAAGTC AGAAGAGCAC ATGGATATGA GGCAAAAGCA CTGCTTGGCC
    TATGTTCTCC ATACGTCAGG CACTACAGGG ATACCTAAGA TTGTCAGAGT CCCTCATGCA
    TGTATAGTGC CAAACATCCA GCATTTTCGG GTGCTTTTTG AAATCACTCA TGAGGATGTT
    TTGTTTCTGG CTTCACCTCT GACCTTTGAT CCTTCTGTGG TGGAAATATT TGTTGCTTTG
    TCAAGTGGTG CCTCTTTGCT TATTGTGCCA ACTTCTGTCA AAATGGTCCC ATCAAAATTA
    GCTGCTGTTC TGTTTTCCCA TCACAGAGTG ACTATTTTAC AGGCAACGCC AACATTGCTT
    AGAAGATTTG GATCTCAGCT CATCAAGTCA ACTGTTTTAT CCGCCAGTAC CTCTCTTCGC
    ATATTGGCCC TTGGTGGTGA AGCATTTCCA TCATTAACTG TTCTCAAAAG CTGGAGAGGA
    GTGGGCAATA AAACACAAAT ATTTAATATT TATGGCATCA CAGAGGTATC AAGTTGGGCG
    ACTTTCTACA GGATTCCAGA GAAGATTCTT AACTCTACTT TGAAGTACGG GTTTCTGTTA
    CTACGAAGAG TAGAGAGAGG ATGTGAATTG CCTGTACAAC TGGGATTCCC ACTGCTTGGA
    ACAGTAGTTG AAGTCAGAGA TACTAACGGT TTCACAATTC AAGAAGGCAA TGGCCAAGTA
    TTTTTAGGTG GCAGAAATAG AGTATGTTTT CTCGATGGCG AAGTGACGGT ACCACTTGGC
    ACAATGAGAG CCACAGGAGA CTTTGTGACT ATAAAAGATG GAGAGATATT TTTCTTGGGA
    CGAAAGGACA GTCAGATCAA ACGTCATGGC AAACGTCTTA ACATAGAACT TGTACAACAG
    ATTGCTGAAG GTCTTCAACA AGTGGAGTCT TGTGCAGTTA CATGGTATAA CCAGGAAAAA
    TTGATTTTGT TCATGGTGAC TAAAAATGAT TTAGTAAAGG ATCACATCTT TGAAGAACTG
    CAGAAACATC TTCCAAGTCA TGCCATCCCA GATGAGCTTG TGTTAATTAA TTCTCTGCCA
    TTTACATCTC ATGGCAAAAT TGATGTTTCT GAGTTAAATA AGATATATTT AAACTACAGA
    GACTTGAAGT CTGAGTGTAA GCTCAGTGGA AAAGAGGAAC TTTGGGAAAA ATTACACCAT
    TTGTGGAAGT CTGTTCTGAG TCTCTCAGAA GATGCTTTGA AGATTCCTGA TGAGTCACTC
    TTCTTAAATA GTGGTGGAGA TTCCTTGAAA TCCATACGGC TTCTCAATGA GATTGAAAAC
    CTTGTTGGCA CATCAGTACC TGGGCTTCTG GAAATTATTC TCAGCAGTTC TATTTTACGG
    ATTTACAATC ACATCCTTCA AACAGTGTTT TCAGATAAAG ATATGACACT TAGCAAGAAT
    CATGCCATGA AAAGAAAATT CAACGTTACT CAAGAGGAAA CCAGTGGAAA ATCTTTACAT
    CAGGAAACTG TCATGCCTTT AAATTGTGAC AAGGAGATCG ATGCTTTTAT TGCACTGAGC
    AGAGGGAATC AGATTTTGTC TTTGAATACT GATAAGTATT TAACTAAGTT GGGACATTGC
    CCTTCAACCT ATTCTTCTGA TTTAAATTCA CAGACTGACA TTCAAAATAG AGAAAGCCTA
    AATACTCCAC CTCTTATTGG GAAGTCAGAA GATCCATCCT GTGTTGTGGA AGTTTCTGAA
    GAGGAAACAC CTGTGATTAG GGCTGAGAAA ATGGAGTTTC GTGTGAGGTG GAGGTCAGAC
    ACAGGCAAAT GTGTGGATGC TTCACCTCTG GTTGTAATAC CAGCTATTGA TAAGTCATCT
    GCAACTGTGT ACATTGGCTC TCATTCTCAT AGAATGATAG CAGTTGATCT TTACTCTGGA
    AAGGTGAAAT GGGAACAGAT TTTAGGAGAT CGCATTGAAT CCTCAGCATG TGTATCTAAG
    TGTGGAAACT TTATTGTAGT GGGCTGTTAT AATGGGTTAG TTTATGTTCT GAAAAGTAAT
    AGTGGAGAAA AATACTGGAT GTTTAGTACT GAGGATGCTG TCAAAAGCTC AGCAACCATG
    GATCCAACCA CAGGACTTCT TTACATTGGA TCTCATGACC AGCATGCATA CGCTTTGGAT
    ATATATGCCA CTGGGGACAG ACTTTGGAAA CATTCCTGTG GAAAGCCACT CTTTTCTTCT
    CCACGGTGTT GCCTACAGTA TGTTTTTATT GGCTGTGTAG ATGGAAATTT ACTCTGCTTT
    ACTCACTTTG GAGAACAGGT TTGGCAATTC TCTACCAATG AACCAATCTT TTCATCCCCA
    TGTACCTCAG CATCAGAGCA AGAAATATTT TTTGGTTCTC ATGACTGCTT TATCTACTGT
    TGTAGTATGA AAGGTCACCT CCAGTGGAAA TTTGAAACTA CTTCAAGAGT GTATTCAACG
    CCTTTTGTTT TCCGTAACCA CAACCATAAC AGTGAAATGT TACTGGCAGC GGCATCTACT
    GATGGGAAAC TGTGGATCTT GGAATCTAAG AGTGGACAGT TGCAAAGTGT GTATGAACTT
    CCTGGAGAAG TTTTCTCTTC TCCTGTGGTC TGGGAATCAA TGCTTATTAT TGGATGTAGA
    AATAATTATG TTTATTGTCT GGATGTATTG GGTGACAAAG GTAATGAAGT GCAGTCCTTA
    GAATATACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90091
    Clone ID Related Accession (Same CDS sequence) XM_015471628.1 , XM_015463765.1
    Accession Version XM_015471628.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3057bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product acyl-CoA synthetase family member 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103975.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAAAAAAT GTAATCTGAA GTATATCCTT GTTGAAAAAG TGCATATAAG TAAATTCAAG 
    TCTTCCTATG AAGCATTATT GAACTATGAT ATGCTTAAAG TAGAACATAA TGACCTAGTA
    CTCTTCAGAC TTCACTGGAA AAATGTCGAG GTGAGCCTGG TGCTAAATGA TAGAAAAGAG
    AAATATGAAA AAGGAAAGAT GCCAAAGAGT ATGCATTCTG AGAGCAGCAG TGAAGAAAAG
    TCAGAAGAGC ACATGGATAT GAGGCAAAAG CACTGCTTGG CCTATGTTCT CCATACGTCA
    GGCACTACAG GGATACCTAA GATTGTCAGA GTCCCTCATG CATGTATAGT GCCAAACATC
    CAGCATTTTC GGGTGCTTTT TGAAATCACT CATGAGGATG TTTTGTTTCT GGCTTCACCT
    CTGACCTTTG ATCCTTCTGT GGTGGAAATA TTTGTTGCTT TGTCAAGTGG TGCCTCTTTG
    CTTATTGTGC CAACTTCTGT CAAAATGGTC CCATCAAAAT TAGCTGCTGT TCTGTTTTCC
    CATCACAGAG TGACTATTTT ACAGGCAACG CCAACATTGC TTAGAAGATT TGGATCTCAG
    CTCATCAAGT CAACTGTTTT ATCCGCCAGT ACCTCTCTTC GCATATTGGC CCTTGGTGGT
    GAAGCATTTC CATCATTAAC TGTTCTCAAA AGCTGGAGAG GAGTGGGCAA TAAAACACAA
    ATATTTAATA TTTATGGCAT CACAGAGGTA TCAAGTTGGG CGACTTTCTA CAGGATTCCA
    GAGAAGATTC TTAACTCTAC TTTGAAGTAC GGGTTTCTGT TACTACGAAG AGTAGAGAGA
    GGATGTGAAT TGCCTGTACA ACTGGGATTC CCACTGCTTG GAACAGTAGT TGAAGTCAGA
    GATACTAACG GTTTCACAAT TCAAGAAGGC AATGGCCAAG TATTTTTAGG TGGCAGAAAT
    AGAGTATGTT TTCTCGATGG CGAAGTGACG GTACCACTTG GCACAATGAG AGCCACAGGA
    GACTTTGTGA CTATAAAAGA TGGAGAGATA TTTTTCTTGG GACGAAAGGA CAGTCAGATC
    AAACGTCATG GCAAACGTCT TAACATAGAA CTTGTACAAC AGATTGCTGA AGGTCTTCAA
    CAAGTGGAGT CTTGTGCAGT TACATGGTAT AACCAGGAAA AATTGATTTT GTTCATGGTG
    ACTAAAAATG ATTTAGTAAA GGATCACATC TTTGAAGAAC TGCAGAAACA TCTTCCAAGT
    CATGCCATCC CAGATGAGCT TGTGTTAATT AATTCTCTGC CATTTACATC TCATGGCAAA
    ATTGATGTTT CTGAGTTAAA TAAGATATAT TTAAACTACA GAGACTTGAA GTCTGAGTGT
    AAGCTCAGTG GAAAAGAGGA ACTTTGGGAA AAATTACACC ATTTGTGGAA GTCTGTTCTG
    AGTCTCTCAG AAGATGCTTT GAAGATTCCT GATGAGTCAC TCTTCTTAAA TAGTGGTGGA
    GATTCCTTGA AATCCATACG GCTTCTCAAT GAGATTGAAA ACCTTGTTGG CACATCAGTA
    CCTGGGCTTC TGGAAATTAT TCTCAGCAGT TCTATTTTAC GGATTTACAA TCACATCCTT
    CAAACAGTGT TTTCAGATAA AGATATGACA CTTAGCAAGA ATCATGCCAT GAAAAGAAAA
    TTCAACGTTA CTCAAGAGGA AACCAGTGGA AAATCTTTAC ATCAGGAAAC TGTCATGCCT
    TTAAATTGTG ACAAGGAGAT CGATGCTTTT ATTGCACTGA GCAGAGGGAA TCAGATTTTG
    TCTTTGAATA CTGATAAGTA TTTAACTAAG TTGGGACATT GCCCTTCAAC CTATTCTTCT
    GATTTAAATT CACAGACTGA CATTCAAAAT AGAGAAAGCC TAAATACTCC ACCTCTTATT
    GGGAAGTCAG AAGATCCATC CTGTGTTGTG GAAGTTTCTG AAGAGGAAAC ACCTGTGATT
    AGGGCTGAGA AAATGGAGTT TCGTGTGAGG TGGAGGTCAG ACACAGGCAA ATGTGTGGAT
    GCTTCACCTC TGGTTGTAAT ACCAGCTATT GATAAGTCAT CTGCAACTGT GTACATTGGC
    TCTCATTCTC ATAGAATGAT AGCAGTTGAT CTTTACTCTG GAAAGGTGAA ATGGGAACAG
    ATTTTAGGAG ATCGCATTGA ATCCTCAGCA TGTGTATCTA AGTGTGGAAA CTTTATTGTA
    GTGGGCTGTT ATAATGGGTT AGTTTATGTT CTGAAAAGTA ATAGTGGAGA AAAATACTGG
    ATGTTTAGTA CTGAGGATGC TGTCAAAAGC TCAGCAACCA TGGATCCAAC CACAGGACTT
    CTTTACATTG GATCTCATGA CCAGCATGCA TACGCTTTGG ATATATATAA GAAGAAGTGT
    GTTTGGAAAT TGAAATGTGG AGGAACTGTT TTTTCTTCTC CTTGTTTGAG CCTGATTCCA
    TCTCACTTGT ATTTTGCTAC ACTGGGAGGA CTTTTACTGG CTATAAATCC TGCCACTGGG
    GACAGACTTT GGAAACATTC CTGTGGAAAG CCACTCTTTT CTTCTCCACG GTGTTGCCTA
    CAGTATGTTT TTATTGGCTG TGTAGATGGA AATTTACTCT GCTTTACTCA CTTTGGAGAA
    CAGGTTTGGC AATTCTCTAC CAATGAACCA ATCTTTTCAT CCCCATGTAC CTCAGCATCA
    GAGCAAGAAA TATTTTTTGG TTCTCATGAC TGCTTTATCT ACTGTTGTAG TATGAAAGGT
    CACCTCCAGT GGAAATTTGA AACTACTTCA AGAGTGTATT CAACGCCTTT TGTTTTCCGT
    AACCACAACC ATAACAGTGA AATGTTACTG GCAGCGGCAT CTACTGATGG GAAACTGTGG
    ATCTTGGAAT CTAAGAGTGG ACAGTTGCAA AGTGTGTATG AACTTCCTGG AGAAGTTTTC
    TCTTCTCCTG TGGTCTGGGA ATCAATGCTT ATTATTGGAT GTAGAAATAA TTATGTTTAT
    TGTCTGGATG TATTGGGTGA CAAAGGTAAT GAAGTGCAGT CCTTAGAATA TACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327114.1
    CDS545..3601
    Translation

    Target ORF information:

    RefSeq Version XM_015471628.1
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471628.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAAAAAAT GTAATCTGAA GTATATCCTT GTTGAAAAAG TGCATATAAG TAAATTCAAG 
    TCTTCCTATG AAGCATTATT GAACTATGAT ATGCTTAAAG TAGAACATAA TGACCTAGTA
    CTCTTCAGAC TTCACTGGAA AAATGTCGAG GTGAGCCTGG TGCTAAATGA TAGAAAAGAG
    AAATATGAAA AAGGAAAGAT GCCAAAGAGT ATGCATTCTG AGAGCAGCAG TGAAGAAAAG
    TCAGAAGAGC ACATGGATAT GAGGCAAAAG CACTGCTTGG CCTATGTTCT CCATACGTCA
    GGCACTACAG GGATACCTAA GATTGTCAGA GTCCCTCATG CATGTATAGT GCCAAACATC
    CAGCATTTTC GGGTGCTTTT TGAAATCACT CATGAGGATG TTTTGTTTCT GGCTTCACCT
    CTGACCTTTG ATCCTTCTGT GGTGGAAATA TTTGTTGCTT TGTCAAGTGG TGCCTCTTTG
    CTTATTGTGC CAACTTCTGT CAAAATGGTC CCATCAAAAT TAGCTGCTGT TCTGTTTTCC
    CATCACAGAG TGACTATTTT ACAGGCAACG CCAACATTGC TTAGAAGATT TGGATCTCAG
    CTCATCAAGT CAACTGTTTT ATCCGCCAGT ACCTCTCTTC GCATATTGGC CCTTGGTGGT
    GAAGCATTTC CATCATTAAC TGTTCTCAAA AGCTGGAGAG GAGTGGGCAA TAAAACACAA
    ATATTTAATA TTTATGGCAT CACAGAGGTA TCAAGTTGGG CGACTTTCTA CAGGATTCCA
    GAGAAGATTC TTAACTCTAC TTTGAAGTAC GGGTTTCTGT TACTACGAAG AGTAGAGAGA
    GGATGTGAAT TGCCTGTACA ACTGGGATTC CCACTGCTTG GAACAGTAGT TGAAGTCAGA
    GATACTAACG GTTTCACAAT TCAAGAAGGC AATGGCCAAG TATTTTTAGG TGGCAGAAAT
    AGAGTATGTT TTCTCGATGG CGAAGTGACG GTACCACTTG GCACAATGAG AGCCACAGGA
    GACTTTGTGA CTATAAAAGA TGGAGAGATA TTTTTCTTGG GACGAAAGGA CAGTCAGATC
    AAACGTCATG GCAAACGTCT TAACATAGAA CTTGTACAAC AGATTGCTGA AGGTCTTCAA
    CAAGTGGAGT CTTGTGCAGT TACATGGTAT AACCAGGAAA AATTGATTTT GTTCATGGTG
    ACTAAAAATG ATTTAGTAAA GGATCACATC TTTGAAGAAC TGCAGAAACA TCTTCCAAGT
    CATGCCATCC CAGATGAGCT TGTGTTAATT AATTCTCTGC CATTTACATC TCATGGCAAA
    ATTGATGTTT CTGAGTTAAA TAAGATATAT TTAAACTACA GAGACTTGAA GTCTGAGTGT
    AAGCTCAGTG GAAAAGAGGA ACTTTGGGAA AAATTACACC ATTTGTGGAA GTCTGTTCTG
    AGTCTCTCAG AAGATGCTTT GAAGATTCCT GATGAGTCAC TCTTCTTAAA TAGTGGTGGA
    GATTCCTTGA AATCCATACG GCTTCTCAAT GAGATTGAAA ACCTTGTTGG CACATCAGTA
    CCTGGGCTTC TGGAAATTAT TCTCAGCAGT TCTATTTTAC GGATTTACAA TCACATCCTT
    CAAACAGTGT TTTCAGATAA AGATATGACA CTTAGCAAGA ATCATGCCAT GAAAAGAAAA
    TTCAACGTTA CTCAAGAGGA AACCAGTGGA AAATCTTTAC ATCAGGAAAC TGTCATGCCT
    TTAAATTGTG ACAAGGAGAT CGATGCTTTT ATTGCACTGA GCAGAGGGAA TCAGATTTTG
    TCTTTGAATA CTGATAAGTA TTTAACTAAG TTGGGACATT GCCCTTCAAC CTATTCTTCT
    GATTTAAATT CACAGACTGA CATTCAAAAT AGAGAAAGCC TAAATACTCC ACCTCTTATT
    GGGAAGTCAG AAGATCCATC CTGTGTTGTG GAAGTTTCTG AAGAGGAAAC ACCTGTGATT
    AGGGCTGAGA AAATGGAGTT TCGTGTGAGG TGGAGGTCAG ACACAGGCAA ATGTGTGGAT
    GCTTCACCTC TGGTTGTAAT ACCAGCTATT GATAAGTCAT CTGCAACTGT GTACATTGGC
    TCTCATTCTC ATAGAATGAT AGCAGTTGAT CTTTACTCTG GAAAGGTGAA ATGGGAACAG
    ATTTTAGGAG ATCGCATTGA ATCCTCAGCA TGTGTATCTA AGTGTGGAAA CTTTATTGTA
    GTGGGCTGTT ATAATGGGTT AGTTTATGTT CTGAAAAGTA ATAGTGGAGA AAAATACTGG
    ATGTTTAGTA CTGAGGATGC TGTCAAAAGC TCAGCAACCA TGGATCCAAC CACAGGACTT
    CTTTACATTG GATCTCATGA CCAGCATGCA TACGCTTTGG ATATATATAA GAAGAAGTGT
    GTTTGGAAAT TGAAATGTGG AGGAACTGTT TTTTCTTCTC CTTGTTTGAG CCTGATTCCA
    TCTCACTTGT ATTTTGCTAC ACTGGGAGGA CTTTTACTGG CTATAAATCC TGCCACTGGG
    GACAGACTTT GGAAACATTC CTGTGGAAAG CCACTCTTTT CTTCTCCACG GTGTTGCCTA
    CAGTATGTTT TTATTGGCTG TGTAGATGGA AATTTACTCT GCTTTACTCA CTTTGGAGAA
    CAGGTTTGGC AATTCTCTAC CAATGAACCA ATCTTTTCAT CCCCATGTAC CTCAGCATCA
    GAGCAAGAAA TATTTTTTGG TTCTCATGAC TGCTTTATCT ACTGTTGTAG TATGAAAGGT
    CACCTCCAGT GGAAATTTGA AACTACTTCA AGAGTGTATT CAACGCCTTT TGTTTTCCGT
    AACCACAACC ATAACAGTGA AATGTTACTG GCAGCGGCAT CTACTGATGG GAAACTGTGG
    ATCTTGGAAT CTAAGAGTGG ACAGTTGCAA AGTGTGTATG AACTTCCTGG AGAAGTTTTC
    TCTTCTCCTG TGGTCTGGGA ATCAATGCTT ATTATTGGAT GTAGAAATAA TTATGTTTAT
    TGTCTGGATG TATTGGGTGA CAAAGGTAAT GAAGTGCAGT CCTTAGAATA TACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90091
    Clone ID Related Accession (Same CDS sequence) XM_015471628.1 , XM_015463765.1
    Accession Version XM_015463765.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3057bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product acyl-CoA synthetase family member 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAAAAAAT GTAATCTGAA GTATATCCTT GTTGAAAAAG TGCATATAAG TAAATTCAAG 
    TCTTCCTATG AAGCATTATT GAACTATGAT ATGCTTAAAG TAGAACATAA TGACCTAGTA
    CTCTTCAGAC TTCACTGGAA AAATGTCGAG GTGAGCCTGG TGCTAAATGA TAGAAAAGAG
    AAATATGAAA AAGGAAAGAT GCCAAAGAGT ATGCATTCTG AGAGCAGCAG TGAAGAAAAG
    TCAGAAGAGC ACATGGATAT GAGGCAAAAG CACTGCTTGG CCTATGTTCT CCATACGTCA
    GGCACTACAG GGATACCTAA GATTGTCAGA GTCCCTCATG CATGTATAGT GCCAAACATC
    CAGCATTTTC GGGTGCTTTT TGAAATCACT CATGAGGATG TTTTGTTTCT GGCTTCACCT
    CTGACCTTTG ATCCTTCTGT GGTGGAAATA TTTGTTGCTT TGTCAAGTGG TGCCTCTTTG
    CTTATTGTGC CAACTTCTGT CAAAATGGTC CCATCAAAAT TAGCTGCTGT TCTGTTTTCC
    CATCACAGAG TGACTATTTT ACAGGCAACG CCAACATTGC TTAGAAGATT TGGATCTCAG
    CTCATCAAGT CAACTGTTTT ATCCGCCAGT ACCTCTCTTC GCATATTGGC CCTTGGTGGT
    GAAGCATTTC CATCATTAAC TGTTCTCAAA AGCTGGAGAG GAGTGGGCAA TAAAACACAA
    ATATTTAATA TTTATGGCAT CACAGAGGTA TCAAGTTGGG CGACTTTCTA CAGGATTCCA
    GAGAAGATTC TTAACTCTAC TTTGAAGTAC GGGTTTCTGT TACTACGAAG AGTAGAGAGA
    GGATGTGAAT TGCCTGTACA ACTGGGATTC CCACTGCTTG GAACAGTAGT TGAAGTCAGA
    GATACTAACG GTTTCACAAT TCAAGAAGGC AATGGCCAAG TATTTTTAGG TGGCAGAAAT
    AGAGTATGTT TTCTCGATGG CGAAGTGACG GTACCACTTG GCACAATGAG AGCCACAGGA
    GACTTTGTGA CTATAAAAGA TGGAGAGATA TTTTTCTTGG GACGAAAGGA CAGTCAGATC
    AAACGTCATG GCAAACGTCT TAACATAGAA CTTGTACAAC AGATTGCTGA AGGTCTTCAA
    CAAGTGGAGT CTTGTGCAGT TACATGGTAT AACCAGGAAA AATTGATTTT GTTCATGGTG
    ACTAAAAATG ATTTAGTAAA GGATCACATC TTTGAAGAAC TGCAGAAACA TCTTCCAAGT
    CATGCCATCC CAGATGAGCT TGTGTTAATT AATTCTCTGC CATTTACATC TCATGGCAAA
    ATTGATGTTT CTGAGTTAAA TAAGATATAT TTAAACTACA GAGACTTGAA GTCTGAGTGT
    AAGCTCAGTG GAAAAGAGGA ACTTTGGGAA AAATTACACC ATTTGTGGAA GTCTGTTCTG
    AGTCTCTCAG AAGATGCTTT GAAGATTCCT GATGAGTCAC TCTTCTTAAA TAGTGGTGGA
    GATTCCTTGA AATCCATACG GCTTCTCAAT GAGATTGAAA ACCTTGTTGG CACATCAGTA
    CCTGGGCTTC TGGAAATTAT TCTCAGCAGT TCTATTTTAC GGATTTACAA TCACATCCTT
    CAAACAGTGT TTTCAGATAA AGATATGACA CTTAGCAAGA ATCATGCCAT GAAAAGAAAA
    TTCAACGTTA CTCAAGAGGA AACCAGTGGA AAATCTTTAC ATCAGGAAAC TGTCATGCCT
    TTAAATTGTG ACAAGGAGAT CGATGCTTTT ATTGCACTGA GCAGAGGGAA TCAGATTTTG
    TCTTTGAATA CTGATAAGTA TTTAACTAAG TTGGGACATT GCCCTTCAAC CTATTCTTCT
    GATTTAAATT CACAGACTGA CATTCAAAAT AGAGAAAGCC TAAATACTCC ACCTCTTATT
    GGGAAGTCAG AAGATCCATC CTGTGTTGTG GAAGTTTCTG AAGAGGAAAC ACCTGTGATT
    AGGGCTGAGA AAATGGAGTT TCGTGTGAGG TGGAGGTCAG ACACAGGCAA ATGTGTGGAT
    GCTTCACCTC TGGTTGTAAT ACCAGCTATT GATAAGTCAT CTGCAACTGT GTACATTGGC
    TCTCATTCTC ATAGAATGAT AGCAGTTGAT CTTTACTCTG GAAAGGTGAA ATGGGAACAG
    ATTTTAGGAG ATCGCATTGA ATCCTCAGCA TGTGTATCTA AGTGTGGAAA CTTTATTGTA
    GTGGGCTGTT ATAATGGGTT AGTTTATGTT CTGAAAAGTA ATAGTGGAGA AAAATACTGG
    ATGTTTAGTA CTGAGGATGC TGTCAAAAGC TCAGCAACCA TGGATCCAAC CACAGGACTT
    CTTTACATTG GATCTCATGA CCAGCATGCA TACGCTTTGG ATATATATAA GAAGAAGTGT
    GTTTGGAAAT TGAAATGTGG AGGAACTGTT TTTTCTTCTC CTTGTTTGAG CCTGATTCCA
    TCTCACTTGT ATTTTGCTAC ACTGGGAGGA CTTTTACTGG CTATAAATCC TGCCACTGGG
    GACAGACTTT GGAAACATTC CTGTGGAAAG CCACTCTTTT CTTCTCCACG GTGTTGCCTA
    CAGTATGTTT TTATTGGCTG TGTAGATGGA AATTTACTCT GCTTTACTCA CTTTGGAGAA
    CAGGTTTGGC AATTCTCTAC CAATGAACCA ATCTTTTCAT CCCCATGTAC CTCAGCATCA
    GAGCAAGAAA TATTTTTTGG TTCTCATGAC TGCTTTATCT ACTGTTGTAG TATGAAAGGT
    CACCTCCAGT GGAAATTTGA AACTACTTCA AGAGTGTATT CAACGCCTTT TGTTTTCCGT
    AACCACAACC ATAACAGTGA AATGTTACTG GCAGCGGCAT CTACTGATGG GAAACTGTGG
    ATCTTGGAAT CTAAGAGTGG ACAGTTGCAA AGTGTGTATG AACTTCCTGG AGAAGTTTTC
    TCTTCTCCTG TGGTCTGGGA ATCAATGCTT ATTATTGGAT GTAGAAATAA TTATGTTTAT
    TGTCTGGATG TATTGGGTGA CAAAGGTAAT GAAGTGCAGT CCTTAGAATA TACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319251.1
    CDS545..3601
    Translation

    Target ORF information:

    RefSeq Version XM_015463765.1
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463765.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAAAAAAT GTAATCTGAA GTATATCCTT GTTGAAAAAG TGCATATAAG TAAATTCAAG 
    TCTTCCTATG AAGCATTATT GAACTATGAT ATGCTTAAAG TAGAACATAA TGACCTAGTA
    CTCTTCAGAC TTCACTGGAA AAATGTCGAG GTGAGCCTGG TGCTAAATGA TAGAAAAGAG
    AAATATGAAA AAGGAAAGAT GCCAAAGAGT ATGCATTCTG AGAGCAGCAG TGAAGAAAAG
    TCAGAAGAGC ACATGGATAT GAGGCAAAAG CACTGCTTGG CCTATGTTCT CCATACGTCA
    GGCACTACAG GGATACCTAA GATTGTCAGA GTCCCTCATG CATGTATAGT GCCAAACATC
    CAGCATTTTC GGGTGCTTTT TGAAATCACT CATGAGGATG TTTTGTTTCT GGCTTCACCT
    CTGACCTTTG ATCCTTCTGT GGTGGAAATA TTTGTTGCTT TGTCAAGTGG TGCCTCTTTG
    CTTATTGTGC CAACTTCTGT CAAAATGGTC CCATCAAAAT TAGCTGCTGT TCTGTTTTCC
    CATCACAGAG TGACTATTTT ACAGGCAACG CCAACATTGC TTAGAAGATT TGGATCTCAG
    CTCATCAAGT CAACTGTTTT ATCCGCCAGT ACCTCTCTTC GCATATTGGC CCTTGGTGGT
    GAAGCATTTC CATCATTAAC TGTTCTCAAA AGCTGGAGAG GAGTGGGCAA TAAAACACAA
    ATATTTAATA TTTATGGCAT CACAGAGGTA TCAAGTTGGG CGACTTTCTA CAGGATTCCA
    GAGAAGATTC TTAACTCTAC TTTGAAGTAC GGGTTTCTGT TACTACGAAG AGTAGAGAGA
    GGATGTGAAT TGCCTGTACA ACTGGGATTC CCACTGCTTG GAACAGTAGT TGAAGTCAGA
    GATACTAACG GTTTCACAAT TCAAGAAGGC AATGGCCAAG TATTTTTAGG TGGCAGAAAT
    AGAGTATGTT TTCTCGATGG CGAAGTGACG GTACCACTTG GCACAATGAG AGCCACAGGA
    GACTTTGTGA CTATAAAAGA TGGAGAGATA TTTTTCTTGG GACGAAAGGA CAGTCAGATC
    AAACGTCATG GCAAACGTCT TAACATAGAA CTTGTACAAC AGATTGCTGA AGGTCTTCAA
    CAAGTGGAGT CTTGTGCAGT TACATGGTAT AACCAGGAAA AATTGATTTT GTTCATGGTG
    ACTAAAAATG ATTTAGTAAA GGATCACATC TTTGAAGAAC TGCAGAAACA TCTTCCAAGT
    CATGCCATCC CAGATGAGCT TGTGTTAATT AATTCTCTGC CATTTACATC TCATGGCAAA
    ATTGATGTTT CTGAGTTAAA TAAGATATAT TTAAACTACA GAGACTTGAA GTCTGAGTGT
    AAGCTCAGTG GAAAAGAGGA ACTTTGGGAA AAATTACACC ATTTGTGGAA GTCTGTTCTG
    AGTCTCTCAG AAGATGCTTT GAAGATTCCT GATGAGTCAC TCTTCTTAAA TAGTGGTGGA
    GATTCCTTGA AATCCATACG GCTTCTCAAT GAGATTGAAA ACCTTGTTGG CACATCAGTA
    CCTGGGCTTC TGGAAATTAT TCTCAGCAGT TCTATTTTAC GGATTTACAA TCACATCCTT
    CAAACAGTGT TTTCAGATAA AGATATGACA CTTAGCAAGA ATCATGCCAT GAAAAGAAAA
    TTCAACGTTA CTCAAGAGGA AACCAGTGGA AAATCTTTAC ATCAGGAAAC TGTCATGCCT
    TTAAATTGTG ACAAGGAGAT CGATGCTTTT ATTGCACTGA GCAGAGGGAA TCAGATTTTG
    TCTTTGAATA CTGATAAGTA TTTAACTAAG TTGGGACATT GCCCTTCAAC CTATTCTTCT
    GATTTAAATT CACAGACTGA CATTCAAAAT AGAGAAAGCC TAAATACTCC ACCTCTTATT
    GGGAAGTCAG AAGATCCATC CTGTGTTGTG GAAGTTTCTG AAGAGGAAAC ACCTGTGATT
    AGGGCTGAGA AAATGGAGTT TCGTGTGAGG TGGAGGTCAG ACACAGGCAA ATGTGTGGAT
    GCTTCACCTC TGGTTGTAAT ACCAGCTATT GATAAGTCAT CTGCAACTGT GTACATTGGC
    TCTCATTCTC ATAGAATGAT AGCAGTTGAT CTTTACTCTG GAAAGGTGAA ATGGGAACAG
    ATTTTAGGAG ATCGCATTGA ATCCTCAGCA TGTGTATCTA AGTGTGGAAA CTTTATTGTA
    GTGGGCTGTT ATAATGGGTT AGTTTATGTT CTGAAAAGTA ATAGTGGAGA AAAATACTGG
    ATGTTTAGTA CTGAGGATGC TGTCAAAAGC TCAGCAACCA TGGATCCAAC CACAGGACTT
    CTTTACATTG GATCTCATGA CCAGCATGCA TACGCTTTGG ATATATATAA GAAGAAGTGT
    GTTTGGAAAT TGAAATGTGG AGGAACTGTT TTTTCTTCTC CTTGTTTGAG CCTGATTCCA
    TCTCACTTGT ATTTTGCTAC ACTGGGAGGA CTTTTACTGG CTATAAATCC TGCCACTGGG
    GACAGACTTT GGAAACATTC CTGTGGAAAG CCACTCTTTT CTTCTCCACG GTGTTGCCTA
    CAGTATGTTT TTATTGGCTG TGTAGATGGA AATTTACTCT GCTTTACTCA CTTTGGAGAA
    CAGGTTTGGC AATTCTCTAC CAATGAACCA ATCTTTTCAT CCCCATGTAC CTCAGCATCA
    GAGCAAGAAA TATTTTTTGG TTCTCATGAC TGCTTTATCT ACTGTTGTAG TATGAAAGGT
    CACCTCCAGT GGAAATTTGA AACTACTTCA AGAGTGTATT CAACGCCTTT TGTTTTCCGT
    AACCACAACC ATAACAGTGA AATGTTACTG GCAGCGGCAT CTACTGATGG GAAACTGTGG
    ATCTTGGAAT CTAAGAGTGG ACAGTTGCAA AGTGTGTATG AACTTCCTGG AGAAGTTTTC
    TCTTCTCCTG TGGTCTGGGA ATCAATGCTT ATTATTGGAT GTAGAAATAA TTATGTTTAT
    TGTCTGGATG TATTGGGTGA CAAAGGTAAT GAAGTGCAGT CCTTAGAATA TACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa04809
    Clone ID Related Accession (Same CDS sequence) XM_001250588.5 , XM_002688264.5
    Accession Version XM_001250588.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3399bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product acyl-CoA synthetase family member 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001250588.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTA AATTCAAGTC TTCCTATGAA GCATTATTGA ACTATGATAT GCTTAAAGTA
    GAACATAATG ACCTAGTACT CTTCAGACTT CACTGGAAAA ATGTCGAGGT GAGCCTGGTG
    CTAAATGATA GAAAAGAGAA ATATGAAAAA GGAAAGATGC CAAAGAGTAT GCATTCTGAG
    AGCAGCAGTG AAGAAAAGTC AGAAGAGCAC ATGGATATGA GGCAAAAGCA CTGCTTGGCC
    TATGTTCTCC ATACGTCAGG CACTACAGGG ATACCTAAGA TTGTCAGAGT CCCTCATGCA
    TGTATAGTGC CAAACATCCA GCATTTTCGG GTGCTTTTTG AAATCACTCA TGAGGATGTT
    TTGTTTCTGG CTTCACCTCT GACCTTTGAT CCTTCTGTGG TGGAAATATT TGTTGCTTTG
    TCAAGTGGTG CCTCTTTGCT TATTGTGCCA ACTTCTGTCA AAATGGTCCC ATCAAAATTA
    GCTGCTGTTC TGTTTTCCCA TCACAGAGTG ACTATTTTAC AGGCAACGCC AACATTGCTT
    AGAAGATTTG GATCTCAGCT CATCAAGTCA ACTGTTTTAT CCGCCAGTAC CTCTCTTCGC
    ATATTGGCCC TTGGTGGTGA AGCATTTCCA TCATTAACTG TTCTCAAAAG CTGGAGAGGA
    GTGGGCAATA AAACACAAAT ATTTAATATT TATGGCATCA CAGAGGTATC AAGTTGGGCG
    ACTTTCTACA GGATTCCAGA GAAGATTCTT AACTCTACTT TGAAATGTGA ATTGCCTGTA
    CAACTGGGAT TCCCACTGCT TGGAACAGTA GTTGAAGTCA GAGATACTAA CGGTTTCACA
    ATTCAAGAAG GCAATGGCCA AGTATTTTTA GGTGGCAGAA ATAGAGTATG TTTTCTCGAT
    GGCGAAGTGA CGGTACCACT TGGCACAATG AGAGCCACAG GAGACTTTGT GACTATAAAA
    GATGGAGAGA TATTTTTCTT GGGACGAAAG GACAGTCAGA TCAAACGTCA TGGCAAACGT
    CTTAACATAG AACTTGTACA ACAGATTGCT GAAGGTCTTC AACAAGTGGA GTCTTGTGCA
    GTTACATGGT ATAACCAGGA AAAATTGATT TTGTTCATGG TGACTAAAAA TGATTTAGTA
    AAGGATCACA TCTTTGAAGA ACTGCAGAAA CATCTTCCAA GTCATGCCAT CCCAGATGAG
    CTTGTGTTAA TTAATTCTCT GCCATTTACA TCTCATGGCA AAATTGATGT TTCTGAGTTA
    AATAAGATAT ATTTAAACTA CAGAGACTTG AAGTCTGAGT GTAAGCTCAG TGGAAAAGAG
    GAACTTTGGG AAAAATTACA CCATTTGTGG AAGTCTGTTC TGAGTCTCTC AGAAGATGCT
    TTGAAGATTC CTGATGAGTC ACTCTTCTTA AATAGTGGTG GAGATTCCTT GAAATCCATA
    CGGCTTCTCA ATGAGATTGA AAACCTTGTT GGCACATCAG TACCTGGGCT TCTGGAAATT
    ATTCTCAGCA GTTCTATTTT ACGGATTTAC AATCACATCC TTCAAACAGT GTTTTCAGAT
    AAAGATATGA CACTTAGCAA GAATCATGCC ATGAAAAGAA AATTCAACGT TACTCAAGAG
    GAAACCAGTG GAAAATCTTT ACATCAGGAA ACTGTCATGC CTTTAAATTG TGACAAGGAG
    ATCGATGCTT TTATTGCACT GAGCAGAGGG AATCAGATTT TGTCTTTGAA TACTGATAAG
    TATTTAACTA AGTTGGGACA TTGCCCTTCA ACCTATTCTT CTGATTTAAA TTCACAGACT
    GACATTCAAA ATAGAGAAAG CCTAAATACT CCACCTCTTA TTGGGAAGTC AGAAGATCCA
    TCCTGTGTTG TGGAAGTTTC TGAAGAGGAA ACACCTGTGA TTAGGGCTGA GAAAATGGAG
    TTTCGTGTGA GGTGGAGGTC AGACACAGGC AAATGTGTGG ATGCTTCACC TCTGGTTGTA
    ATACCAGCTA TTGATAAGTC ATCTGCAACT GTGTACATTG GCTCTCATTC TCATAGAATG
    ATAGCAGTTG ATCTTTACTC TGGAAAGGTG AAATGGGAAC AGATTTTAGG AGATCGCATT
    GAATCCTCAG CATGTGTATC TAAGTGTGGA AACTTTATTG TAGTGGGCTG TTATAATGGG
    TTAGTTTATG TTCTGAAAAG TAATAGTGGA GAAAAATACT GGATGTTTAG TACTGAGGAT
    GCTGTCAAAA GCTCAGCAAC CATGGATCCA ACCACAGGAC TTCTTTACAT TGGATCTCAT
    GACCAGCATG CATACGCTTT GGATATATAT AAGAAGAAGT GTGTTTGGAA ATTGAAATGT
    GGAGGAACTG TTTTTTCTTC TCCTTGTTTG AGCCTGATTC CATCTCACTT GTATTTTGCT
    ACACTGGGAG GACTTTTACT GGCTATAAAT CCTGCCACTG GGGACAGACT TTGGAAACAT
    TCCTGTGGAA AGCCACTCTT TTCTTCTCCA CGGTGTTGCC TACAGTATGT TTTTATTGGC
    TGTGTAGATG GAAATTTACT CTGCTTTACT CACTTTGGAG AACAGGTTTG GCAATTCTCT
    ACCAATGAAC CAATCTTTTC ATCCCCATGT ACCTCAGCAT CAGAGCAAGA AATATTTTTT
    GGTTCTCATG ACTGCTTTAT CTACTGTTGT AGTATGAAAG GTCACCTCCA GTGGAAATTT
    GAAACTACTT CAAGAGTGTA TTCAACGCCT TTTGTTTTCC GTAACCACAA CCATAACAGT
    GAAATGTTAC TGGCAGCGGC ATCTACTGAT GGGAAACTGT GGATCTTGGA ATCTAAGAGT
    GGACAGTTGC AAAGTGTGTA TGAACTTCCT GGAGAAGTTT TCTCTTCTCC TGTGGTCTGG
    GAATCAATGC TTATTATTGG ATGTAGAAAT AATTATGTTT ATTGTCTGGA TGTATTGGGT
    GACAAAGGTA ATGAAGTGCA GTCCTTAGAA TATACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001250589.2
    CDS438..3836
    Translation

    Target ORF information:

    RefSeq Version XM_001250588.5
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001250588.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTA AATTCAAGTC TTCCTATGAA GCATTATTGA ACTATGATAT GCTTAAAGTA
    GAACATAATG ACCTAGTACT CTTCAGACTT CACTGGAAAA ATGTCGAGGT GAGCCTGGTG
    CTAAATGATA GAAAAGAGAA ATATGAAAAA GGAAAGATGC CAAAGAGTAT GCATTCTGAG
    AGCAGCAGTG AAGAAAAGTC AGAAGAGCAC ATGGATATGA GGCAAAAGCA CTGCTTGGCC
    TATGTTCTCC ATACGTCAGG CACTACAGGG ATACCTAAGA TTGTCAGAGT CCCTCATGCA
    TGTATAGTGC CAAACATCCA GCATTTTCGG GTGCTTTTTG AAATCACTCA TGAGGATGTT
    TTGTTTCTGG CTTCACCTCT GACCTTTGAT CCTTCTGTGG TGGAAATATT TGTTGCTTTG
    TCAAGTGGTG CCTCTTTGCT TATTGTGCCA ACTTCTGTCA AAATGGTCCC ATCAAAATTA
    GCTGCTGTTC TGTTTTCCCA TCACAGAGTG ACTATTTTAC AGGCAACGCC AACATTGCTT
    AGAAGATTTG GATCTCAGCT CATCAAGTCA ACTGTTTTAT CCGCCAGTAC CTCTCTTCGC
    ATATTGGCCC TTGGTGGTGA AGCATTTCCA TCATTAACTG TTCTCAAAAG CTGGAGAGGA
    GTGGGCAATA AAACACAAAT ATTTAATATT TATGGCATCA CAGAGGTATC AAGTTGGGCG
    ACTTTCTACA GGATTCCAGA GAAGATTCTT AACTCTACTT TGAAATGTGA ATTGCCTGTA
    CAACTGGGAT TCCCACTGCT TGGAACAGTA GTTGAAGTCA GAGATACTAA CGGTTTCACA
    ATTCAAGAAG GCAATGGCCA AGTATTTTTA GGTGGCAGAA ATAGAGTATG TTTTCTCGAT
    GGCGAAGTGA CGGTACCACT TGGCACAATG AGAGCCACAG GAGACTTTGT GACTATAAAA
    GATGGAGAGA TATTTTTCTT GGGACGAAAG GACAGTCAGA TCAAACGTCA TGGCAAACGT
    CTTAACATAG AACTTGTACA ACAGATTGCT GAAGGTCTTC AACAAGTGGA GTCTTGTGCA
    GTTACATGGT ATAACCAGGA AAAATTGATT TTGTTCATGG TGACTAAAAA TGATTTAGTA
    AAGGATCACA TCTTTGAAGA ACTGCAGAAA CATCTTCCAA GTCATGCCAT CCCAGATGAG
    CTTGTGTTAA TTAATTCTCT GCCATTTACA TCTCATGGCA AAATTGATGT TTCTGAGTTA
    AATAAGATAT ATTTAAACTA CAGAGACTTG AAGTCTGAGT GTAAGCTCAG TGGAAAAGAG
    GAACTTTGGG AAAAATTACA CCATTTGTGG AAGTCTGTTC TGAGTCTCTC AGAAGATGCT
    TTGAAGATTC CTGATGAGTC ACTCTTCTTA AATAGTGGTG GAGATTCCTT GAAATCCATA
    CGGCTTCTCA ATGAGATTGA AAACCTTGTT GGCACATCAG TACCTGGGCT TCTGGAAATT
    ATTCTCAGCA GTTCTATTTT ACGGATTTAC AATCACATCC TTCAAACAGT GTTTTCAGAT
    AAAGATATGA CACTTAGCAA GAATCATGCC ATGAAAAGAA AATTCAACGT TACTCAAGAG
    GAAACCAGTG GAAAATCTTT ACATCAGGAA ACTGTCATGC CTTTAAATTG TGACAAGGAG
    ATCGATGCTT TTATTGCACT GAGCAGAGGG AATCAGATTT TGTCTTTGAA TACTGATAAG
    TATTTAACTA AGTTGGGACA TTGCCCTTCA ACCTATTCTT CTGATTTAAA TTCACAGACT
    GACATTCAAA ATAGAGAAAG CCTAAATACT CCACCTCTTA TTGGGAAGTC AGAAGATCCA
    TCCTGTGTTG TGGAAGTTTC TGAAGAGGAA ACACCTGTGA TTAGGGCTGA GAAAATGGAG
    TTTCGTGTGA GGTGGAGGTC AGACACAGGC AAATGTGTGG ATGCTTCACC TCTGGTTGTA
    ATACCAGCTA TTGATAAGTC ATCTGCAACT GTGTACATTG GCTCTCATTC TCATAGAATG
    ATAGCAGTTG ATCTTTACTC TGGAAAGGTG AAATGGGAAC AGATTTTAGG AGATCGCATT
    GAATCCTCAG CATGTGTATC TAAGTGTGGA AACTTTATTG TAGTGGGCTG TTATAATGGG
    TTAGTTTATG TTCTGAAAAG TAATAGTGGA GAAAAATACT GGATGTTTAG TACTGAGGAT
    GCTGTCAAAA GCTCAGCAAC CATGGATCCA ACCACAGGAC TTCTTTACAT TGGATCTCAT
    GACCAGCATG CATACGCTTT GGATATATAT AAGAAGAAGT GTGTTTGGAA ATTGAAATGT
    GGAGGAACTG TTTTTTCTTC TCCTTGTTTG AGCCTGATTC CATCTCACTT GTATTTTGCT
    ACACTGGGAG GACTTTTACT GGCTATAAAT CCTGCCACTG GGGACAGACT TTGGAAACAT
    TCCTGTGGAA AGCCACTCTT TTCTTCTCCA CGGTGTTGCC TACAGTATGT TTTTATTGGC
    TGTGTAGATG GAAATTTACT CTGCTTTACT CACTTTGGAG AACAGGTTTG GCAATTCTCT
    ACCAATGAAC CAATCTTTTC ATCCCCATGT ACCTCAGCAT CAGAGCAAGA AATATTTTTT
    GGTTCTCATG ACTGCTTTAT CTACTGTTGT AGTATGAAAG GTCACCTCCA GTGGAAATTT
    GAAACTACTT CAAGAGTGTA TTCAACGCCT TTTGTTTTCC GTAACCACAA CCATAACAGT
    GAAATGTTAC TGGCAGCGGC ATCTACTGAT GGGAAACTGT GGATCTTGGA ATCTAAGAGT
    GGACAGTTGC AAAGTGTGTA TGAACTTCCT GGAGAAGTTT TCTCTTCTCC TGTGGTCTGG
    GAATCAATGC TTATTATTGG ATGTAGAAAT AATTATGTTT ATTGTCTGGA TGTATTGGGT
    GACAAAGGTA ATGAAGTGCA GTCCTTAGAA TATACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa04809
    Clone ID Related Accession (Same CDS sequence) XM_001250588.5 , XM_002688264.5
    Accession Version XM_002688264.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3399bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product acyl-CoA synthetase family member 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103975.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002688264.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTA AATTCAAGTC TTCCTATGAA GCATTATTGA ACTATGATAT GCTTAAAGTA
    GAACATAATG ACCTAGTACT CTTCAGACTT CACTGGAAAA ATGTCGAGGT GAGCCTGGTG
    CTAAATGATA GAAAAGAGAA ATATGAAAAA GGAAAGATGC CAAAGAGTAT GCATTCTGAG
    AGCAGCAGTG AAGAAAAGTC AGAAGAGCAC ATGGATATGA GGCAAAAGCA CTGCTTGGCC
    TATGTTCTCC ATACGTCAGG CACTACAGGG ATACCTAAGA TTGTCAGAGT CCCTCATGCA
    TGTATAGTGC CAAACATCCA GCATTTTCGG GTGCTTTTTG AAATCACTCA TGAGGATGTT
    TTGTTTCTGG CTTCACCTCT GACCTTTGAT CCTTCTGTGG TGGAAATATT TGTTGCTTTG
    TCAAGTGGTG CCTCTTTGCT TATTGTGCCA ACTTCTGTCA AAATGGTCCC ATCAAAATTA
    GCTGCTGTTC TGTTTTCCCA TCACAGAGTG ACTATTTTAC AGGCAACGCC AACATTGCTT
    AGAAGATTTG GATCTCAGCT CATCAAGTCA ACTGTTTTAT CCGCCAGTAC CTCTCTTCGC
    ATATTGGCCC TTGGTGGTGA AGCATTTCCA TCATTAACTG TTCTCAAAAG CTGGAGAGGA
    GTGGGCAATA AAACACAAAT ATTTAATATT TATGGCATCA CAGAGGTATC AAGTTGGGCG
    ACTTTCTACA GGATTCCAGA GAAGATTCTT AACTCTACTT TGAAATGTGA ATTGCCTGTA
    CAACTGGGAT TCCCACTGCT TGGAACAGTA GTTGAAGTCA GAGATACTAA CGGTTTCACA
    ATTCAAGAAG GCAATGGCCA AGTATTTTTA GGTGGCAGAA ATAGAGTATG TTTTCTCGAT
    GGCGAAGTGA CGGTACCACT TGGCACAATG AGAGCCACAG GAGACTTTGT GACTATAAAA
    GATGGAGAGA TATTTTTCTT GGGACGAAAG GACAGTCAGA TCAAACGTCA TGGCAAACGT
    CTTAACATAG AACTTGTACA ACAGATTGCT GAAGGTCTTC AACAAGTGGA GTCTTGTGCA
    GTTACATGGT ATAACCAGGA AAAATTGATT TTGTTCATGG TGACTAAAAA TGATTTAGTA
    AAGGATCACA TCTTTGAAGA ACTGCAGAAA CATCTTCCAA GTCATGCCAT CCCAGATGAG
    CTTGTGTTAA TTAATTCTCT GCCATTTACA TCTCATGGCA AAATTGATGT TTCTGAGTTA
    AATAAGATAT ATTTAAACTA CAGAGACTTG AAGTCTGAGT GTAAGCTCAG TGGAAAAGAG
    GAACTTTGGG AAAAATTACA CCATTTGTGG AAGTCTGTTC TGAGTCTCTC AGAAGATGCT
    TTGAAGATTC CTGATGAGTC ACTCTTCTTA AATAGTGGTG GAGATTCCTT GAAATCCATA
    CGGCTTCTCA ATGAGATTGA AAACCTTGTT GGCACATCAG TACCTGGGCT TCTGGAAATT
    ATTCTCAGCA GTTCTATTTT ACGGATTTAC AATCACATCC TTCAAACAGT GTTTTCAGAT
    AAAGATATGA CACTTAGCAA GAATCATGCC ATGAAAAGAA AATTCAACGT TACTCAAGAG
    GAAACCAGTG GAAAATCTTT ACATCAGGAA ACTGTCATGC CTTTAAATTG TGACAAGGAG
    ATCGATGCTT TTATTGCACT GAGCAGAGGG AATCAGATTT TGTCTTTGAA TACTGATAAG
    TATTTAACTA AGTTGGGACA TTGCCCTTCA ACCTATTCTT CTGATTTAAA TTCACAGACT
    GACATTCAAA ATAGAGAAAG CCTAAATACT CCACCTCTTA TTGGGAAGTC AGAAGATCCA
    TCCTGTGTTG TGGAAGTTTC TGAAGAGGAA ACACCTGTGA TTAGGGCTGA GAAAATGGAG
    TTTCGTGTGA GGTGGAGGTC AGACACAGGC AAATGTGTGG ATGCTTCACC TCTGGTTGTA
    ATACCAGCTA TTGATAAGTC ATCTGCAACT GTGTACATTG GCTCTCATTC TCATAGAATG
    ATAGCAGTTG ATCTTTACTC TGGAAAGGTG AAATGGGAAC AGATTTTAGG AGATCGCATT
    GAATCCTCAG CATGTGTATC TAAGTGTGGA AACTTTATTG TAGTGGGCTG TTATAATGGG
    TTAGTTTATG TTCTGAAAAG TAATAGTGGA GAAAAATACT GGATGTTTAG TACTGAGGAT
    GCTGTCAAAA GCTCAGCAAC CATGGATCCA ACCACAGGAC TTCTTTACAT TGGATCTCAT
    GACCAGCATG CATACGCTTT GGATATATAT AAGAAGAAGT GTGTTTGGAA ATTGAAATGT
    GGAGGAACTG TTTTTTCTTC TCCTTGTTTG AGCCTGATTC CATCTCACTT GTATTTTGCT
    ACACTGGGAG GACTTTTACT GGCTATAAAT CCTGCCACTG GGGACAGACT TTGGAAACAT
    TCCTGTGGAA AGCCACTCTT TTCTTCTCCA CGGTGTTGCC TACAGTATGT TTTTATTGGC
    TGTGTAGATG GAAATTTACT CTGCTTTACT CACTTTGGAG AACAGGTTTG GCAATTCTCT
    ACCAATGAAC CAATCTTTTC ATCCCCATGT ACCTCAGCAT CAGAGCAAGA AATATTTTTT
    GGTTCTCATG ACTGCTTTAT CTACTGTTGT AGTATGAAAG GTCACCTCCA GTGGAAATTT
    GAAACTACTT CAAGAGTGTA TTCAACGCCT TTTGTTTTCC GTAACCACAA CCATAACAGT
    GAAATGTTAC TGGCAGCGGC ATCTACTGAT GGGAAACTGT GGATCTTGGA ATCTAAGAGT
    GGACAGTTGC AAAGTGTGTA TGAACTTCCT GGAGAAGTTT TCTCTTCTCC TGTGGTCTGG
    GAATCAATGC TTATTATTGG ATGTAGAAAT AATTATGTTT ATTGTCTGGA TGTATTGGGT
    GACAAAGGTA ATGAAGTGCA GTCCTTAGAA TATACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002688310.1
    CDS438..3836
    Translation

    Target ORF information:

    RefSeq Version XM_002688264.5
    Organism Bos taurus(cattle)
    Definition Bos taurus aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002688264.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATTTCGA GAAGCAATTG GAAAAACGTG TTGAAAGAGT TCTCTGCTAC CAGTGATGTT 
    CAAATAAGCG TCAGTGAAAT GACTCTTCAG GAATTGGTGC ATCAGGCTGC CTCCCTTTAT
    TCAGATAAAA TAGCTGTGTG TTTTGATGAA TGTAACAACC AGCCTCCAGT TTACTATACC
    TACAAGACTG TGATTCATGC TGCTTCAGCA TTATCGAATT TTCTGCTGTT ACACTGTGAG
    TTTCAAGGAA TTCGGGAAAT TGGTCTCTAC TGCCATCCTA CAATAAACTT ACCCTCTTGG
    ATTTTAGGAA TTCTCCAAGT CCCTGCTGCT TATGTACCTG TTGATCCAGA TTCACCACCA
    GCATTATCAG TTCATTTTAT GAAAAAATGT AATCTGAAGT ATATCCTTGT TGAAAAAGTG
    CATATAAGTA AATTCAAGTC TTCCTATGAA GCATTATTGA ACTATGATAT GCTTAAAGTA
    GAACATAATG ACCTAGTACT CTTCAGACTT CACTGGAAAA ATGTCGAGGT GAGCCTGGTG
    CTAAATGATA GAAAAGAGAA ATATGAAAAA GGAAAGATGC CAAAGAGTAT GCATTCTGAG
    AGCAGCAGTG AAGAAAAGTC AGAAGAGCAC ATGGATATGA GGCAAAAGCA CTGCTTGGCC
    TATGTTCTCC ATACGTCAGG CACTACAGGG ATACCTAAGA TTGTCAGAGT CCCTCATGCA
    TGTATAGTGC CAAACATCCA GCATTTTCGG GTGCTTTTTG AAATCACTCA TGAGGATGTT
    TTGTTTCTGG CTTCACCTCT GACCTTTGAT CCTTCTGTGG TGGAAATATT TGTTGCTTTG
    TCAAGTGGTG CCTCTTTGCT TATTGTGCCA ACTTCTGTCA AAATGGTCCC ATCAAAATTA
    GCTGCTGTTC TGTTTTCCCA TCACAGAGTG ACTATTTTAC AGGCAACGCC AACATTGCTT
    AGAAGATTTG GATCTCAGCT CATCAAGTCA ACTGTTTTAT CCGCCAGTAC CTCTCTTCGC
    ATATTGGCCC TTGGTGGTGA AGCATTTCCA TCATTAACTG TTCTCAAAAG CTGGAGAGGA
    GTGGGCAATA AAACACAAAT ATTTAATATT TATGGCATCA CAGAGGTATC AAGTTGGGCG
    ACTTTCTACA GGATTCCAGA GAAGATTCTT AACTCTACTT TGAAATGTGA ATTGCCTGTA
    CAACTGGGAT TCCCACTGCT TGGAACAGTA GTTGAAGTCA GAGATACTAA CGGTTTCACA
    ATTCAAGAAG GCAATGGCCA AGTATTTTTA GGTGGCAGAA ATAGAGTATG TTTTCTCGAT
    GGCGAAGTGA CGGTACCACT TGGCACAATG AGAGCCACAG GAGACTTTGT GACTATAAAA
    GATGGAGAGA TATTTTTCTT GGGACGAAAG GACAGTCAGA TCAAACGTCA TGGCAAACGT
    CTTAACATAG AACTTGTACA ACAGATTGCT GAAGGTCTTC AACAAGTGGA GTCTTGTGCA
    GTTACATGGT ATAACCAGGA AAAATTGATT TTGTTCATGG TGACTAAAAA TGATTTAGTA
    AAGGATCACA TCTTTGAAGA ACTGCAGAAA CATCTTCCAA GTCATGCCAT CCCAGATGAG
    CTTGTGTTAA TTAATTCTCT GCCATTTACA TCTCATGGCA AAATTGATGT TTCTGAGTTA
    AATAAGATAT ATTTAAACTA CAGAGACTTG AAGTCTGAGT GTAAGCTCAG TGGAAAAGAG
    GAACTTTGGG AAAAATTACA CCATTTGTGG AAGTCTGTTC TGAGTCTCTC AGAAGATGCT
    TTGAAGATTC CTGATGAGTC ACTCTTCTTA AATAGTGGTG GAGATTCCTT GAAATCCATA
    CGGCTTCTCA ATGAGATTGA AAACCTTGTT GGCACATCAG TACCTGGGCT TCTGGAAATT
    ATTCTCAGCA GTTCTATTTT ACGGATTTAC AATCACATCC TTCAAACAGT GTTTTCAGAT
    AAAGATATGA CACTTAGCAA GAATCATGCC ATGAAAAGAA AATTCAACGT TACTCAAGAG
    GAAACCAGTG GAAAATCTTT ACATCAGGAA ACTGTCATGC CTTTAAATTG TGACAAGGAG
    ATCGATGCTT TTATTGCACT GAGCAGAGGG AATCAGATTT TGTCTTTGAA TACTGATAAG
    TATTTAACTA AGTTGGGACA TTGCCCTTCA ACCTATTCTT CTGATTTAAA TTCACAGACT
    GACATTCAAA ATAGAGAAAG CCTAAATACT CCACCTCTTA TTGGGAAGTC AGAAGATCCA
    TCCTGTGTTG TGGAAGTTTC TGAAGAGGAA ACACCTGTGA TTAGGGCTGA GAAAATGGAG
    TTTCGTGTGA GGTGGAGGTC AGACACAGGC AAATGTGTGG ATGCTTCACC TCTGGTTGTA
    ATACCAGCTA TTGATAAGTC ATCTGCAACT GTGTACATTG GCTCTCATTC TCATAGAATG
    ATAGCAGTTG ATCTTTACTC TGGAAAGGTG AAATGGGAAC AGATTTTAGG AGATCGCATT
    GAATCCTCAG CATGTGTATC TAAGTGTGGA AACTTTATTG TAGTGGGCTG TTATAATGGG
    TTAGTTTATG TTCTGAAAAG TAATAGTGGA GAAAAATACT GGATGTTTAG TACTGAGGAT
    GCTGTCAAAA GCTCAGCAAC CATGGATCCA ACCACAGGAC TTCTTTACAT TGGATCTCAT
    GACCAGCATG CATACGCTTT GGATATATAT AAGAAGAAGT GTGTTTGGAA ATTGAAATGT
    GGAGGAACTG TTTTTTCTTC TCCTTGTTTG AGCCTGATTC CATCTCACTT GTATTTTGCT
    ACACTGGGAG GACTTTTACT GGCTATAAAT CCTGCCACTG GGGACAGACT TTGGAAACAT
    TCCTGTGGAA AGCCACTCTT TTCTTCTCCA CGGTGTTGCC TACAGTATGT TTTTATTGGC
    TGTGTAGATG GAAATTTACT CTGCTTTACT CACTTTGGAG AACAGGTTTG GCAATTCTCT
    ACCAATGAAC CAATCTTTTC ATCCCCATGT ACCTCAGCAT CAGAGCAAGA AATATTTTTT
    GGTTCTCATG ACTGCTTTAT CTACTGTTGT AGTATGAAAG GTCACCTCCA GTGGAAATTT
    GAAACTACTT CAAGAGTGTA TTCAACGCCT TTTGTTTTCC GTAACCACAA CCATAACAGT
    GAAATGTTAC TGGCAGCGGC ATCTACTGAT GGGAAACTGT GGATCTTGGA ATCTAAGAGT
    GGACAGTTGC AAAGTGTGTA TGAACTTCCT GGAGAAGTTT TCTCTTCTCC TGTGGTCTGG
    GAATCAATGC TTATTATTGG ATGTAGAAAT AATTATGTTT ATTGTCTGGA TGTATTGGGT
    GACAAAGGTA ATGAAGTGCA GTCCTTAGAA TATACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.