NOTCH1 cDNA ORF clone, Bos taurus(cattle)

The following NOTCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOTCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa220988 XM_024999642.1
Latest version!
Bos taurus notch 1 (NOTCH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa220989 XM_024999643.1
Latest version!
Bos taurus notch 1 (NOTCH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa220990 XM_024999644.1
Latest version!
Bos taurus notch 1 (NOTCH1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa56852 XM_010810569.1
Latest version!
Bos taurus notch 1 (NOTCH1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa94696 XM_015465548.1
Latest version!
Bos taurus notch 1 (NOTCH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OBa94697 XM_015465549.1
Latest version!
Bos taurus notch 1 (NOTCH1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa94698 XM_015465550.1
Latest version!
Bos taurus notch 1 (NOTCH1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa220988
    Clone ID Related Accession (Same CDS sequence) XM_024999642.1
    Accession Version XM_024999642.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7713bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurogenic locus notch homolog protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGGCA TTTTGGACGC CCAGCACTTA ACCCGGGCTG GCACAGGATG CCCAAATCTG 
    GCGCTGTGGT GCAGAAATAA GTCACCCGGA AGGAGCACGG GGGAGGGTCC AGGGCTGCCT
    GACCCACAGC GTGCTTCGCG GGCCACCTTA GGCTGGAGGC GCGGGAGAAG GGGCCTGCGC
    TGCTCCCAGC CCGGTGAGAC CTGCTTGAAC GGTGGGAAGT GTGAAGTGTT CCCCAACGGC
    ACCGAGGCCT GCATCTGCGG CGGGGCCTTC GCGGGCCAGC AATGCCAGGC CCCCAACCCC
    TGCCTCAGCG CCCCCTGCAA GAACGGCGGG ACGTGCCACA CGACGGAGCG AGAAGGCCTG
    GTGGACTATG TCTGCGGCTG CCGCCTGGGC TTCTCGGGGC CACTGTGCCT GACGCCCCGC
    GACCACGCTT GCCTCGCCAG CCCCTGCCTC AACGGTGGCA CCTGTGATCT GCTCACGCTC
    ACTGAGTACA AGTGCCTGTG CACCCCGGGC TGGTCAGGGA AGACGTGCCA GCAGGCCGAC
    CCCTGCGCCT CCAACCCCTG TGCCAACGGT GGCCAGTGCC TGCCCTTTGA GGCGTCGTAT
    ATCTGCCACT GCCCGCCCGG CTTCCACGGC CCCACGTGCA GGCAGGACGT CAACGAGTGC
    AGCCAGAGCC CCGGGCTCTG CCACCACGGC GGCACCTGCC TCAACGAGGT CGGCTCCTAC
    CGCTGCGTCT GCCGCCCCAC CCACACCGGC CCCCACTGCG AGCTGCCCTA CGTGCCCTGC
    AGTCCCTCCC CCTGCCAGAA CGGGGGCACC TGCCGCCCCA CGGGGGACAC CACCCACGAG
    TGCGCCTGCC TGCCAGGCTT CACTGGCCAG AACTGTGAGG AAAACATTGA CGACTGTCCG
    GGGAACAGCT GCAAGAACGG AGGCGCCTGT GTGGACGGCG TGAACACCTA CAACTGCCGC
    TGCCCCCCGG AGTGGACAGG TCAGTACTGC ACAGAGGACG TGGACGAGTG CCAGCTCATG
    CCCAACGCCT GCCAGAACGG CGGGACCTGC CACAACACCC ACGGCGGCTA CAACTGCGTG
    TGCGTCAACG GCTGGACGGG CGAGGACTGC AGCGAGAACA TCGACGACTG CGCCAGCGCC
    TCCTGCTTCC AGGGCGCCAC CTGCCACGAC CGCGTCGCCT CCTTCTACTG CGAGTGTCCC
    CACGGCCGCA CGGGTCTGCT GTGCCACCTG AACGATGCCT GCATCAGCAA CCCCTGCAAC
    GAGGGCTCCA ACTGCGACAC CAACCCCGTC AACGGCAAGG CCATCTGCAC CTGCCCCTCG
    GGGTACACGG GGCCGGCCTG CAGCCAGGAT GTGGACGAGT GCTCGCTGGG CGCCAACCCC
    TGTGAGCACG CGGGCAAGTG CATCAACACG CTGGGCTCGT TCGAGTGCCA GTGTCTGCAG
    GGCTACACCG GCCCGCGCTG TGAGATCGAC GTCAACGAGT GTGTATCGAA CCCGTGTCAG
    AACGACGCCA CTTGTCTGGA TCAGATCGGG GAGTTCCAGT GTATCTGCAT GCCTGGCTAC
    GAGGGCTTGC ACTGCGAGGT GAACACGGAC GAGTGTGCCA GCAGCCCCTG CCTGCAGAAC
    GGCCGCTGCC TGGACAAGAT CAACGAGTTC GTGTGCGAGT GCCCCACTGG CTTCACCGGG
    CACTTGTGCC AGTATGACGT GGACGAGTGT GCAAGCACAC CCTGCAAGAA CGGCGCTAAG
    TGCCTGGACG GGCCCAACAC CTACACCTGC GTGTGCACAG AAGGCTACAC GGGGCCCCAC
    TGCGAGGTGG ACATCGACGA GTGTGACCCC GACCCCTGCC ACTACGGGTC CTGCAAGGAC
    GGCGTGGCCA CCTTCACCTG CCTGTGCCAG CCGGGCTACA CGGGCCACCA CTGTGAGAGC
    AACATTAACG AGTGCCACAG CCAGCCCTGC CGCCACGGGG GCACCTGCCA GGACCGCGAC
    AACGCCTATC TCTGCTTCTG CCTCAAGGGG ACCACAGGAC CCAACTGCGA GATCAACCTG
    GATGACTGCG CAAGCAACCC TTGCGACTCC GGCACGTGTC TGGACAAGAT TGACGGCTAC
    GAGTGTGCCT GTGAGCCAGG CTACACAGGG AGTATGTGTA ACATCAACAT TGACGAGTGT
    GCGGACAGCC CTTGCCACAA CGGGGGCACC TGCGAGGATG GCATCAACGG CTTCACCTGT
    CGCTGCCCCG AGGGCTACCA CGACCCCACC TGCCTGTCTG AGGTCAACGA GTGCAGCAGC
    AACCCCTGCA TCCATGGGGC CTGCCGCGAC AGCCTCAACG GGTACAAGTG TGACTGTGAC
    CCCGGGTGGA GTGGGGCCAA CTGTGACGTC AACAACGACG AGTGTGAGTC GAACCCCTGC
    ATCAACGGGG GCACCTGCAA GGACATGACC AGCGGCTACG TGTGCGCCTG CCGCGAGGGC
    TTCAGTGGGC CCAACTGCCA GACCAACATC AACGAGTGCG CATCCAACCC ATGTCTGAAC
    CAGGGCACGT GTATCGATGA CGTGGCGGGG TACAAATGCA ACTGCCTCTT GCCCTACACA
    GGGGCCACGT GTGAGGTGGT GCTGGCCCCA TGTGCCCCCG GGCCCTGCAG AAACGGCGGT
    GAGTGTCGGG AGTCAGAGGA CTATGAGAGC TTCTCCTGTG CCTGCCCCGC GGGCTGGCAG
    GGGCAGACCT GCGAGATCGA CATCAACGAG TGCGTGAAGA GCCCGTGCCG CGCGGGCGCC
    TCCTGCCAGA ACACCAACGG CAGCTACCGC TGCCACTGCC AGGCGGGCTA CACGGGGCGC
    AACTGCGAGA CGGACATCGA CGACTGCCGG CCCAACCCGT GCCACAATGG GGGCTCCTGC
    ACAGATGGCA TCAACACGGC CTTCTGCGAC TGCCTGCCCG GCTTCCAGGG CGCCTTCTGC
    GAGGAGGACA TCAACGAGTG CGCCAGCAGC CCCTGCCGCA ACGGCGCCAA CTGCACCGAC
    TGTGTGGACA GCTACACCTG CACCTGCCCC ACGGGCTTCA GCGGCATCCA CTGCGAGAAC
    AACACGCCCG ACTGCACGGA GAGCTCCTGC TTCAACGGCG GTACCTGCGT GGATGGCATC
    AACTCCTTCA CCTGCCTGTG TCCACCTGGC TTCACGGGCA GCTACTGCCA ACATGACGTC
    AACGAGTGTG ACTCTCGGCC CTGCCTGCAC GGCGGCACCT GCCACGACAG CTACGGCACC
    TACACGTGCA CCTGCCCGCA GGGCTACACA GGCCTCAACT GCCAGACCCT CGTGCGCTGG
    TGCGACTCGT CCCCCTGCAA GAATGACGGC CGGTGCTGGC AGACCAACGC GCTCTACCGC
    TGTGAGTGCC ACAGCGGCTG GACCGGCCTC TACTGTGACG TGCCCAGCGT CTCCTGCGAG
    GTGGCCGCGC GGCAGCAAGG CGTCAACGTG ACCCACTTGT GCCGGAACGG GGGCCTCTGC
    ATGAATGCGG GCAACACACA CCGCTGCCAC TGCCAGGCCG GCTACACGGG CAGCTACTGC
    GAGGAGCAGG TGGACGAGTG CTCGCCCAGC CCCTGCCAGA ACGGGGCCAC CTGCACCGAC
    TACCCCGGCG GCTACTCCTG TGAGTGTGTG GCCGGCTACC ACGGGGTGAA CTGCTCCGAG
    GAGGTGAACG AGTGCCTGTC CCAGCCCTGC CGGAACGGAG GCACCTGCAT CGACCTCACC
    AACACCTACA AGTGTTCCTG CCCCCGCGGC ACACAGGGCG TGCACTGCGA GATCAACGTG
    GACGACTGCA ACCCCCCCAT CGACCCCGTG TCCCGGGGCC CCAAGTGCTT CAACAACGGC
    ACCTGCGTGG ACCAGGTGGG CGGCTACAGC TGCAGCTGCC CGCCCGGCTT CGTGGGTGAG
    CGCTGCGAGG GGGATGTCAA CGAGTGCCTG TCCAACCCCT GCGACGCCCG TGGCACCCAG
    AACTGCGTGC AGCACGTCAA CGCCTTCCAC TGCGAGTGCC GCGCCGGCCA CACCGGACGC
    CGCTGCGAGT CAGTCATCAA CGGCTGCAAA GACAGGCCCT GTAAGAATGG GGGTAGCTGT
    GCAGTGGCCT CCAACACGGC CCGTGGGTTC ATCTGCAAGT GCCCAGCGGG CTTCGAGGGC
    GCCACGTGCG AGAACGACGC CCGCAGCTGC GGGAGCCTGC GGTGCCTGAA CGGCGGCACG
    TGCATCGCGG GCCCGCGCAG CCCCACCTGC CTGTGCCTGG GCCCCTTCAC CGGCCCCGAG
    TGCCAGTTCC CGGCCAGCAG CCCCTGCGTG GGCGGCAACC CCTGCTACAA CCAGGGCGTC
    TGTGAGCCCA CCGCCGAGAG CCCCTTCTAC CGCTGCCGCT GCCCCGCCAA GTTCAACGGG
    CTCCTGTGCC ACATCCTGGA CTACAGCTTC GGGGGCGGCG TGGGCCTCGA CATCCCCCCG
    CCACAGATCG AGGAGACCTG CGAGCTCCCT GGCTGCCGCG AGGAGGCCGG CAACAAGGTC
    TGCAGCCTGC AGTGCAACAG CCACGCGTGC GGCTGGGACG GCGGCGACTG CTCCCTCGAC
    TTCGACGACC CTTGGCAGAA CTGCACGCAG TCCCTGCAGT GCTGGAAGTA CTTCAGCAAC
    GGCCGCTGCG ACAGCCAGTG CAACTCGGCC GGCTGCCTCT TCGACGGCTT CGACTGTCAG
    CGCGCAGAAG GCCAGTGCAA CCCCCTGTAC GACCAGTACT GCAAGGACCA CTTCAGGGAC
    GGGCACTGCG ACCAGGGCTG CAACAGTGCG GAGTGCGAGT GGGACGGGCT GGACTGTGCG
    GAGCATGTGC CCGAGCGGCT GGCGGCCGGC ACGCTGGTGC TGGTGGTGCT CATGCCGCCC
    GAGCAGCTGC GCAACCGCTC CCTGCACTTC CTGCGCGAGC TCAGCCGCCT GCTGCACACC
    AATGTGGTCT TCAAGCGTGA TGCCAGTGGC CAGCAGATGA TCTTCCCCTA CTACGGCCGC
    GAGGAGGAGC TGCACAAGCA CCCCGTCAGG CGCTCCGTGG ACGGTGGGGC CCCACTGCTC
    CTGGGCGGCG CGGGCAGACG CCAGCGCAGG GAGCTGGACC CCATGGACAT CCGCGGGTCC
    ATCGTCTACC TGGAGATCGA CAACCGGCAG TGCGTCCAGT CATCCTCCCA GTGCTTCCAG
    AGCGCCACGG ACGTGGCCGC CTTCTTGGGC GCGCTGGCCT CGCTGGGCAG CCTTAACATC
    CCCTACAAGA TCGAGGCTGT GCAGAGTGAG ACGGTGGAGC CGCCCCCGCC GCCGCCGCTG
    CACTTCATGT ATGTGGCTGT GGTGGCCTTC GTGTTGCTGT TCTTCGTGGG CTGCGGGGTG
    CTGCTGTCGC GGAAGCGCCG GCGGCAGCAT GGCCAGCTCT GGTTCCCTGA GGGCTTCAAG
    GTGTCGGAGG CCAGCAAGAA GAAGCGGCGC GAGCCCCTCG GCGAGGACTC CGTGGGCCTC
    AAGCCCCTGA AGAACTCCTC GGACGGCGCC CTCATGGACG ACAACCAGAA CGAGTGGGGC
    GACGAGGGCC TGGAGGCCAA GAAGTTCCGG TTCGAGGAGC CCGTGGTCCT CCCTGACCTG
    GACGACCAGA CGGACCACCG GCAGTGGACC CAGCAGCACC TGGACGCCGC CGACCTGCGC
    GTGTCTGCCA TGGCCCCCAC GCCTCCCCAG GGCGAGGCGG ATGCCGACTG CATGGACGTG
    AACGTCCGCG GGCCGGACGG CTTCACGCCC CTCATGATCG CCTCCTGCAG CGGAGGGGGC
    CTAGAGACTG GCAACAGCGA GGAGGAGGAG GACGCGCCCG CGGTCATCTC GGACTTCATC
    TACCAGGGCG CCAGCCTGCA CAACCAGACG GACCGCACCG GCGAGACCGC CCTGCACCTG
    GCCGCCCGCT ACTCGCGCTC CGACGCGGCC AAGCGCCTGC TGGAGGCCAG CGCGGACGCC
    AACATACAGG ACAACATGGG GCGCACCCCG CTGCACGCGG CCGTGTCTGC CGACGCACAG
    GGCGTTTTCC AGATCCTGAT CCGGAACCGA GCCACCGACC TGGATGCCCG CATGCACGAC
    GGCACGACCC CGCTGATCCT CGCTGCCCGC CTGGCCGTGG AGGGCATGCT GGAGGACCTC
    ATCAACTCTC ACGCCGACGT CAACGCTGTG GATGACCTGG GCAAGTCCGC CCTGCACTGG
    GCAGCCGCGG TGAACAACGT GGAGGCTGCT GTCGTGCTGC TGAAGAACGG GGCCAACAAG
    GACATGCAGA ACAACAAGGA GGAGACGCCC TTGTTCCTGG CCGCCCGGGA GGGCAGCTAC
    GAGACCGCCA AGGTGCTGCT GGACCATTTT GCCAACCGGG ACATCACAGA CCACATGGAC
    CGCCTGCCTC GCGACATCGC GCAGGAGCGT ATGCACCACG ACATCGTGCG GCTGCTGGAC
    GAGTACAGCC TGGTGCGCAG CCCCCCGCTG CATGGCGCCA CCCTGGGCGG CACGCCCACC
    CTGTCGCCCC CGCTCTGCTC GCCCAACGGC TACCTGGGCA ACCTCAAGCC CCCCATGCAG
    GGCAAGAAGG CCCGCAAGCC CAGCACCAAG GGCCTGGCCT GCGGCGGCAA GGAGCCCAAG
    GACCTCAAGG CTCGGAGGAA GAAGTCCCAA GATGGCAAGG GCTGTCTGCT GGACAGCGGG
    AGCGTGATGT CGCCCGTGGA CTCCCTCGAG TCCCCCCACG GCTACCTGTC AGACGTGGCC
    TCCCCACCCC TCCTGCCCTC CCCTTTCCAG CCGTCTCCCT CCGTGCCCCT CAACCACCTG
    CCCGGGATGC CCGAGACCCA CCTGGGCGTC AGCCACCTGA GCGTGGCGGC CAAGCCCGAG
    ATGGCGGTGC TGAGCGGGGG CAGCCGGCTG GCCTTCGAGG CAGGGCCACC ACGCCTCTCC
    CACCTGCCCG TGGCCTCCAG CACCAGCACG ATCCTGGGTT CGGGCGGCAG TGGGGGCAGT
    GGGGCTGTGA ATTTCACCGT GGGTGGAGCT GCGGGCTTGA ATGGCCAGTG CGAGTGGCTG
    TCCCGGCTGC AGAACGGCCT GGTGCCCAAC CAGTACAACC CGCTGCGAGG GGGCGTGACG
    CCGGGCACCC TGAGCACGCA GGCTGCTGGC CTCCAGCATG GCACGGTGGG CCCGCTGCAC
    GCCCCCGCCC TGTCCCAGGT GATGACCTAC CAGGCCCTGC CCAGCACGCG GCTGGCCTCC
    CAGCCTCACC TGGTGCAGCC GCAGCAGAAC TTACAGATGC AGCCGCCGAG CATGCCGCCG
    CAGCCAAACC TGCAGCCGCA CCTCGGCGTA AGCTCGGCCG CCAGCGGCCA CCTGGGCCGG
    AGCTTCCTGG GAGGGGAGCT GAGCCAGGCG GACATGCAGC CGCTGGGGCC CGGCAACCTG
    GCGGCGCACA CCGTCCTGCC GCAGGACGGC CAGGTCCTGC CCACATCGCT GCCGTCCACG
    CTGGCCCCGC CCACGATGGC CCCGCCCATG ACCACCGCCC AGTTCCTGAC GCCCCCCTCC
    CAGCACAGCT ACTCGTCCTC CCCCGTGGAC AACACCCCCA GCCACCAGCT ACAGGTGCCC
    GAGCACCCCT TCCTCACCCC GTCCCCGGAG TCCCCCGACC AGTGGTCCAG CTCCTCGCCA
    CATTCCAACA TCTCGGATTG GTCCGAGGGC ATCTCCAGCC CGCCCACCAG CGTGCCGTCC
    CAGATCGCCC ACGTCCCGGA GGCATTCAAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855410.1
    CDS34..7746
    Translation

    Target ORF information:

    RefSeq Version XM_024999642.1
    Organism Bos taurus(cattle)
    Definition Bos taurus notch 1 (NOTCH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999642.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGGCA TTTTGGACGC CCAGCACTTA ACCCGGGCTG GCACAGGATG CCCAAATCTG 
    GCGCTGTGGT GCAGAAATAA GTCACCCGGA AGGAGCACGG GGGAGGGTCC AGGGCTGCCT
    GACCCACAGC GTGCTTCGCG GGCCACCTTA GGCTGGAGGC GCGGGAGAAG GGGCCTGCGC
    TGCTCCCAGC CCGGTGAGAC CTGCTTGAAC GGTGGGAAGT GTGAAGTGTT CCCCAACGGC
    ACCGAGGCCT GCATCTGCGG CGGGGCCTTC GCGGGCCAGC AATGCCAGGC CCCCAACCCC
    TGCCTCAGCG CCCCCTGCAA GAACGGCGGG ACGTGCCACA CGACGGAGCG AGAAGGCCTG
    GTGGACTATG TCTGCGGCTG CCGCCTGGGC TTCTCGGGGC CACTGTGCCT GACGCCCCGC
    GACCACGCTT GCCTCGCCAG CCCCTGCCTC AACGGTGGCA CCTGTGATCT GCTCACGCTC
    ACTGAGTACA AGTGCCTGTG CACCCCGGGC TGGTCAGGGA AGACGTGCCA GCAGGCCGAC
    CCCTGCGCCT CCAACCCCTG TGCCAACGGT GGCCAGTGCC TGCCCTTTGA GGCGTCGTAT
    ATCTGCCACT GCCCGCCCGG CTTCCACGGC CCCACGTGCA GGCAGGACGT CAACGAGTGC
    AGCCAGAGCC CCGGGCTCTG CCACCACGGC GGCACCTGCC TCAACGAGGT CGGCTCCTAC
    CGCTGCGTCT GCCGCCCCAC CCACACCGGC CCCCACTGCG AGCTGCCCTA CGTGCCCTGC
    AGTCCCTCCC CCTGCCAGAA CGGGGGCACC TGCCGCCCCA CGGGGGACAC CACCCACGAG
    TGCGCCTGCC TGCCAGGCTT CACTGGCCAG AACTGTGAGG AAAACATTGA CGACTGTCCG
    GGGAACAGCT GCAAGAACGG AGGCGCCTGT GTGGACGGCG TGAACACCTA CAACTGCCGC
    TGCCCCCCGG AGTGGACAGG TCAGTACTGC ACAGAGGACG TGGACGAGTG CCAGCTCATG
    CCCAACGCCT GCCAGAACGG CGGGACCTGC CACAACACCC ACGGCGGCTA CAACTGCGTG
    TGCGTCAACG GCTGGACGGG CGAGGACTGC AGCGAGAACA TCGACGACTG CGCCAGCGCC
    TCCTGCTTCC AGGGCGCCAC CTGCCACGAC CGCGTCGCCT CCTTCTACTG CGAGTGTCCC
    CACGGCCGCA CGGGTCTGCT GTGCCACCTG AACGATGCCT GCATCAGCAA CCCCTGCAAC
    GAGGGCTCCA ACTGCGACAC CAACCCCGTC AACGGCAAGG CCATCTGCAC CTGCCCCTCG
    GGGTACACGG GGCCGGCCTG CAGCCAGGAT GTGGACGAGT GCTCGCTGGG CGCCAACCCC
    TGTGAGCACG CGGGCAAGTG CATCAACACG CTGGGCTCGT TCGAGTGCCA GTGTCTGCAG
    GGCTACACCG GCCCGCGCTG TGAGATCGAC GTCAACGAGT GTGTATCGAA CCCGTGTCAG
    AACGACGCCA CTTGTCTGGA TCAGATCGGG GAGTTCCAGT GTATCTGCAT GCCTGGCTAC
    GAGGGCTTGC ACTGCGAGGT GAACACGGAC GAGTGTGCCA GCAGCCCCTG CCTGCAGAAC
    GGCCGCTGCC TGGACAAGAT CAACGAGTTC GTGTGCGAGT GCCCCACTGG CTTCACCGGG
    CACTTGTGCC AGTATGACGT GGACGAGTGT GCAAGCACAC CCTGCAAGAA CGGCGCTAAG
    TGCCTGGACG GGCCCAACAC CTACACCTGC GTGTGCACAG AAGGCTACAC GGGGCCCCAC
    TGCGAGGTGG ACATCGACGA GTGTGACCCC GACCCCTGCC ACTACGGGTC CTGCAAGGAC
    GGCGTGGCCA CCTTCACCTG CCTGTGCCAG CCGGGCTACA CGGGCCACCA CTGTGAGAGC
    AACATTAACG AGTGCCACAG CCAGCCCTGC CGCCACGGGG GCACCTGCCA GGACCGCGAC
    AACGCCTATC TCTGCTTCTG CCTCAAGGGG ACCACAGGAC CCAACTGCGA GATCAACCTG
    GATGACTGCG CAAGCAACCC TTGCGACTCC GGCACGTGTC TGGACAAGAT TGACGGCTAC
    GAGTGTGCCT GTGAGCCAGG CTACACAGGG AGTATGTGTA ACATCAACAT TGACGAGTGT
    GCGGACAGCC CTTGCCACAA CGGGGGCACC TGCGAGGATG GCATCAACGG CTTCACCTGT
    CGCTGCCCCG AGGGCTACCA CGACCCCACC TGCCTGTCTG AGGTCAACGA GTGCAGCAGC
    AACCCCTGCA TCCATGGGGC CTGCCGCGAC AGCCTCAACG GGTACAAGTG TGACTGTGAC
    CCCGGGTGGA GTGGGGCCAA CTGTGACGTC AACAACGACG AGTGTGAGTC GAACCCCTGC
    ATCAACGGGG GCACCTGCAA GGACATGACC AGCGGCTACG TGTGCGCCTG CCGCGAGGGC
    TTCAGTGGGC CCAACTGCCA GACCAACATC AACGAGTGCG CATCCAACCC ATGTCTGAAC
    CAGGGCACGT GTATCGATGA CGTGGCGGGG TACAAATGCA ACTGCCTCTT GCCCTACACA
    GGGGCCACGT GTGAGGTGGT GCTGGCCCCA TGTGCCCCCG GGCCCTGCAG AAACGGCGGT
    GAGTGTCGGG AGTCAGAGGA CTATGAGAGC TTCTCCTGTG CCTGCCCCGC GGGCTGGCAG
    GGGCAGACCT GCGAGATCGA CATCAACGAG TGCGTGAAGA GCCCGTGCCG CGCGGGCGCC
    TCCTGCCAGA ACACCAACGG CAGCTACCGC TGCCACTGCC AGGCGGGCTA CACGGGGCGC
    AACTGCGAGA CGGACATCGA CGACTGCCGG CCCAACCCGT GCCACAATGG GGGCTCCTGC
    ACAGATGGCA TCAACACGGC CTTCTGCGAC TGCCTGCCCG GCTTCCAGGG CGCCTTCTGC
    GAGGAGGACA TCAACGAGTG CGCCAGCAGC CCCTGCCGCA ACGGCGCCAA CTGCACCGAC
    TGTGTGGACA GCTACACCTG CACCTGCCCC ACGGGCTTCA GCGGCATCCA CTGCGAGAAC
    AACACGCCCG ACTGCACGGA GAGCTCCTGC TTCAACGGCG GTACCTGCGT GGATGGCATC
    AACTCCTTCA CCTGCCTGTG TCCACCTGGC TTCACGGGCA GCTACTGCCA ACATGACGTC
    AACGAGTGTG ACTCTCGGCC CTGCCTGCAC GGCGGCACCT GCCACGACAG CTACGGCACC
    TACACGTGCA CCTGCCCGCA GGGCTACACA GGCCTCAACT GCCAGACCCT CGTGCGCTGG
    TGCGACTCGT CCCCCTGCAA GAATGACGGC CGGTGCTGGC AGACCAACGC GCTCTACCGC
    TGTGAGTGCC ACAGCGGCTG GACCGGCCTC TACTGTGACG TGCCCAGCGT CTCCTGCGAG
    GTGGCCGCGC GGCAGCAAGG CGTCAACGTG ACCCACTTGT GCCGGAACGG GGGCCTCTGC
    ATGAATGCGG GCAACACACA CCGCTGCCAC TGCCAGGCCG GCTACACGGG CAGCTACTGC
    GAGGAGCAGG TGGACGAGTG CTCGCCCAGC CCCTGCCAGA ACGGGGCCAC CTGCACCGAC
    TACCCCGGCG GCTACTCCTG TGAGTGTGTG GCCGGCTACC ACGGGGTGAA CTGCTCCGAG
    GAGGTGAACG AGTGCCTGTC CCAGCCCTGC CGGAACGGAG GCACCTGCAT CGACCTCACC
    AACACCTACA AGTGTTCCTG CCCCCGCGGC ACACAGGGCG TGCACTGCGA GATCAACGTG
    GACGACTGCA ACCCCCCCAT CGACCCCGTG TCCCGGGGCC CCAAGTGCTT CAACAACGGC
    ACCTGCGTGG ACCAGGTGGG CGGCTACAGC TGCAGCTGCC CGCCCGGCTT CGTGGGTGAG
    CGCTGCGAGG GGGATGTCAA CGAGTGCCTG TCCAACCCCT GCGACGCCCG TGGCACCCAG
    AACTGCGTGC AGCACGTCAA CGCCTTCCAC TGCGAGTGCC GCGCCGGCCA CACCGGACGC
    CGCTGCGAGT CAGTCATCAA CGGCTGCAAA GACAGGCCCT GTAAGAATGG GGGTAGCTGT
    GCAGTGGCCT CCAACACGGC CCGTGGGTTC ATCTGCAAGT GCCCAGCGGG CTTCGAGGGC
    GCCACGTGCG AGAACGACGC CCGCAGCTGC GGGAGCCTGC GGTGCCTGAA CGGCGGCACG
    TGCATCGCGG GCCCGCGCAG CCCCACCTGC CTGTGCCTGG GCCCCTTCAC CGGCCCCGAG
    TGCCAGTTCC CGGCCAGCAG CCCCTGCGTG GGCGGCAACC CCTGCTACAA CCAGGGCGTC
    TGTGAGCCCA CCGCCGAGAG CCCCTTCTAC CGCTGCCGCT GCCCCGCCAA GTTCAACGGG
    CTCCTGTGCC ACATCCTGGA CTACAGCTTC GGGGGCGGCG TGGGCCTCGA CATCCCCCCG
    CCACAGATCG AGGAGACCTG CGAGCTCCCT GGCTGCCGCG AGGAGGCCGG CAACAAGGTC
    TGCAGCCTGC AGTGCAACAG CCACGCGTGC GGCTGGGACG GCGGCGACTG CTCCCTCGAC
    TTCGACGACC CTTGGCAGAA CTGCACGCAG TCCCTGCAGT GCTGGAAGTA CTTCAGCAAC
    GGCCGCTGCG ACAGCCAGTG CAACTCGGCC GGCTGCCTCT TCGACGGCTT CGACTGTCAG
    CGCGCAGAAG GCCAGTGCAA CCCCCTGTAC GACCAGTACT GCAAGGACCA CTTCAGGGAC
    GGGCACTGCG ACCAGGGCTG CAACAGTGCG GAGTGCGAGT GGGACGGGCT GGACTGTGCG
    GAGCATGTGC CCGAGCGGCT GGCGGCCGGC ACGCTGGTGC TGGTGGTGCT CATGCCGCCC
    GAGCAGCTGC GCAACCGCTC CCTGCACTTC CTGCGCGAGC TCAGCCGCCT GCTGCACACC
    AATGTGGTCT TCAAGCGTGA TGCCAGTGGC CAGCAGATGA TCTTCCCCTA CTACGGCCGC
    GAGGAGGAGC TGCACAAGCA CCCCGTCAGG CGCTCCGTGG ACGGTGGGGC CCCACTGCTC
    CTGGGCGGCG CGGGCAGACG CCAGCGCAGG GAGCTGGACC CCATGGACAT CCGCGGGTCC
    ATCGTCTACC TGGAGATCGA CAACCGGCAG TGCGTCCAGT CATCCTCCCA GTGCTTCCAG
    AGCGCCACGG ACGTGGCCGC CTTCTTGGGC GCGCTGGCCT CGCTGGGCAG CCTTAACATC
    CCCTACAAGA TCGAGGCTGT GCAGAGTGAG ACGGTGGAGC CGCCCCCGCC GCCGCCGCTG
    CACTTCATGT ATGTGGCTGT GGTGGCCTTC GTGTTGCTGT TCTTCGTGGG CTGCGGGGTG
    CTGCTGTCGC GGAAGCGCCG GCGGCAGCAT GGCCAGCTCT GGTTCCCTGA GGGCTTCAAG
    GTGTCGGAGG CCAGCAAGAA GAAGCGGCGC GAGCCCCTCG GCGAGGACTC CGTGGGCCTC
    AAGCCCCTGA AGAACTCCTC GGACGGCGCC CTCATGGACG ACAACCAGAA CGAGTGGGGC
    GACGAGGGCC TGGAGGCCAA GAAGTTCCGG TTCGAGGAGC CCGTGGTCCT CCCTGACCTG
    GACGACCAGA CGGACCACCG GCAGTGGACC CAGCAGCACC TGGACGCCGC CGACCTGCGC
    GTGTCTGCCA TGGCCCCCAC GCCTCCCCAG GGCGAGGCGG ATGCCGACTG CATGGACGTG
    AACGTCCGCG GGCCGGACGG CTTCACGCCC CTCATGATCG CCTCCTGCAG CGGAGGGGGC
    CTAGAGACTG GCAACAGCGA GGAGGAGGAG GACGCGCCCG CGGTCATCTC GGACTTCATC
    TACCAGGGCG CCAGCCTGCA CAACCAGACG GACCGCACCG GCGAGACCGC CCTGCACCTG
    GCCGCCCGCT ACTCGCGCTC CGACGCGGCC AAGCGCCTGC TGGAGGCCAG CGCGGACGCC
    AACATACAGG ACAACATGGG GCGCACCCCG CTGCACGCGG CCGTGTCTGC CGACGCACAG
    GGCGTTTTCC AGATCCTGAT CCGGAACCGA GCCACCGACC TGGATGCCCG CATGCACGAC
    GGCACGACCC CGCTGATCCT CGCTGCCCGC CTGGCCGTGG AGGGCATGCT GGAGGACCTC
    ATCAACTCTC ACGCCGACGT CAACGCTGTG GATGACCTGG GCAAGTCCGC CCTGCACTGG
    GCAGCCGCGG TGAACAACGT GGAGGCTGCT GTCGTGCTGC TGAAGAACGG GGCCAACAAG
    GACATGCAGA ACAACAAGGA GGAGACGCCC TTGTTCCTGG CCGCCCGGGA GGGCAGCTAC
    GAGACCGCCA AGGTGCTGCT GGACCATTTT GCCAACCGGG ACATCACAGA CCACATGGAC
    CGCCTGCCTC GCGACATCGC GCAGGAGCGT ATGCACCACG ACATCGTGCG GCTGCTGGAC
    GAGTACAGCC TGGTGCGCAG CCCCCCGCTG CATGGCGCCA CCCTGGGCGG CACGCCCACC
    CTGTCGCCCC CGCTCTGCTC GCCCAACGGC TACCTGGGCA ACCTCAAGCC CCCCATGCAG
    GGCAAGAAGG CCCGCAAGCC CAGCACCAAG GGCCTGGCCT GCGGCGGCAA GGAGCCCAAG
    GACCTCAAGG CTCGGAGGAA GAAGTCCCAA GATGGCAAGG GCTGTCTGCT GGACAGCGGG
    AGCGTGATGT CGCCCGTGGA CTCCCTCGAG TCCCCCCACG GCTACCTGTC AGACGTGGCC
    TCCCCACCCC TCCTGCCCTC CCCTTTCCAG CCGTCTCCCT CCGTGCCCCT CAACCACCTG
    CCCGGGATGC CCGAGACCCA CCTGGGCGTC AGCCACCTGA GCGTGGCGGC CAAGCCCGAG
    ATGGCGGTGC TGAGCGGGGG CAGCCGGCTG GCCTTCGAGG CAGGGCCACC ACGCCTCTCC
    CACCTGCCCG TGGCCTCCAG CACCAGCACG ATCCTGGGTT CGGGCGGCAG TGGGGGCAGT
    GGGGCTGTGA ATTTCACCGT GGGTGGAGCT GCGGGCTTGA ATGGCCAGTG CGAGTGGCTG
    TCCCGGCTGC AGAACGGCCT GGTGCCCAAC CAGTACAACC CGCTGCGAGG GGGCGTGACG
    CCGGGCACCC TGAGCACGCA GGCTGCTGGC CTCCAGCATG GCACGGTGGG CCCGCTGCAC
    GCCCCCGCCC TGTCCCAGGT GATGACCTAC CAGGCCCTGC CCAGCACGCG GCTGGCCTCC
    CAGCCTCACC TGGTGCAGCC GCAGCAGAAC TTACAGATGC AGCCGCCGAG CATGCCGCCG
    CAGCCAAACC TGCAGCCGCA CCTCGGCGTA AGCTCGGCCG CCAGCGGCCA CCTGGGCCGG
    AGCTTCCTGG GAGGGGAGCT GAGCCAGGCG GACATGCAGC CGCTGGGGCC CGGCAACCTG
    GCGGCGCACA CCGTCCTGCC GCAGGACGGC CAGGTCCTGC CCACATCGCT GCCGTCCACG
    CTGGCCCCGC CCACGATGGC CCCGCCCATG ACCACCGCCC AGTTCCTGAC GCCCCCCTCC
    CAGCACAGCT ACTCGTCCTC CCCCGTGGAC AACACCCCCA GCCACCAGCT ACAGGTGCCC
    GAGCACCCCT TCCTCACCCC GTCCCCGGAG TCCCCCGACC AGTGGTCCAG CTCCTCGCCA
    CATTCCAACA TCTCGGATTG GTCCGAGGGC ATCTCCAGCC CGCCCACCAG CGTGCCGTCC
    CAGATCGCCC ACGTCCCGGA GGCATTCAAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220989
    Clone ID Related Accession (Same CDS sequence) XM_024999643.1
    Accession Version XM_024999643.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurogenic locus notch homolog protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGCCGCCGC TCCTGGCGCC CCTGCTTTGC CTGGCGTTGC TGCCTGCGCT CGCCGCACGA 
    GGCCTGCGCT GCTCCCAGCC CGGTGAGACC TGCTTGAACG GTGGGAAGTG TGAAGTGTTC
    CCCAACGGCA CCGAGGCCTG CATCTGCGGC GGGGCCTTCG CGGGCCAGCA ATGCCAGGCC
    CCCAACCCCT GCCTCAGCGC CCCCTGCAAG AACGGCGGGA CGTGCCACAC GACGGAGCGA
    GAAGGCCTGG TGGACTATGT CTGCGGCTGC CGCCTGGGCT TCTCGGGGCC ACTGTGCCTG
    ACGCCCCGCG ACCACGCTTG CCTCGCCAGC CCCTGCCTCA ACGGTGGCAC CTGTGATCTG
    CTCACGCTCA CTGAGTACAA GTGCCTGTGC ACCCCGGGCT GGTCAGGGAA GACGTGCCAG
    CAGGCCGACC CCTGCGCCTC CAACCCCTGT GCCAACGGTG GCCAGTGCCT GCCCTTTGAG
    GCGTCGTATA TCTGCCACTG CCCGCCCGGC TTCCACGGCC CCACGTGCAG GCAGGACGTC
    AACGAGTGCA GCCAGAGCCC CGGGCTCTGC CACCACGGCG GCACCTGCCT CAACGAGGTC
    GGCTCCTACC GCTGCGTCTG CCGCCCCACC CACACCGGCC CCCACTGCGA GCTGCCCTAC
    GTGCCCTGCA GTCCCTCCCC CTGCCAGAAC GGGGGCACCT GCCGCCCCAC GGGGGACACC
    ACCCACGAGT GCGCCTGCCT GCCAGGCTTC ACTGGCCAGA ACTGTGAGGA AAACATTGAC
    GACTGTCCGG GGAACAGCTG CAAGAACGGA GGCGCCTGTG TGGACGGCGT GAACACCTAC
    AACTGCCGCT GCCCCCCGGA GTGGACAGGT CAGTACTGCA CAGAGGACGT GGACGAGTGC
    CAGCTCATGC CCAACGCCTG CCAGAACGGC GGGACCTGCC ACAACACCCA CGGCGGCTAC
    AACTGCGTGT GCGTCAACGG CTGGACGGGC GAGGACTGCA GCGAGAACAT CGACGACTGC
    GCCAGCGCCT CCTGCTTCCA GGGCGCCACC TGCCACGACC GCGTCGCCTC CTTCTACTGC
    GAGTGTCCCC ACGGCCGCAC GGGTCTGCTG TGCCACCTGA ACGATGCCTG CATCAGCAAC
    CCCTGCAACG AGGGCTCCAA CTGCGACACC AACCCCGTCA ACGGCAAGGC CATCTGCACC
    TGCCCCTCGG GGTACACGGG GCCGGCCTGC AGCCAGGATG TGGACGAGTG CTCGCTGGGC
    GCCAACCCCT GTGAGCACGC GGGCAAGTGC ATCAACACGC TGGGCTCGTT CGAGTGCCAG
    TGTCTGCAGG GCTACACCGG CCCGCGCTGT GAGATCGACG TCAACGAGTG TGTATCGAAC
    CCGTGTCAGA ACGACGCCAC TTGTCTGGAT CAGATCGGGG AGTTCCAGTG TATCTGCATG
    CCTGGCTACG AGGGCTTGCA CTGCGAGGTG AACACGGACG AGTGTGCCAG CAGCCCCTGC
    CTGCAGAACG GCCGCTGCCT GGACAAGATC AACGAGTTCG TGTGCGAGTG CCCCACTGGC
    TTCACCGGGC ACTTGTGCCA GTATGACGTG GACGAGTGTG CAAGCACACC CTGCAAGAAC
    GGCGCTAAGT GCCTGGACGG GCCCAACACC TACACCTGCG TGTGCACAGA AGGCTACACG
    GGGCCCCACT GCGAGGTGGA CATCGACGAG TGTGACCCCG ACCCCTGCCA CTACGGGTCC
    TGCAAGGACG GCGTGGCCAC CTTCACCTGC CTGTGCCAGC CGGGCTACAC GGGCCACCAC
    TGTGAGAGCA ACATTAACGA GTGCCACAGC CAGCCCTGCC GCCACGGGGG CACCTGCCAG
    GACCGCGACA ACGCCTATCT CTGCTTCTGC CTCAAGGGGA CCACAGGACC CAACTGCGAG
    ATCAACCTGG ATGACTGCGC AAGCAACCCT TGCGACTCCG GCACGTGTCT GGACAAGATT
    GACGGCTACG AGTGTGCCTG TGAGCCAGGC TACACAGGGA GTATGTGTAA CATCAACATT
    GACGAGTGTG CGGACAGCCC TTGCCACAAC GGGGGCACCT GCGAGGATGG CATCAACGGC
    TTCACCTGTC GCTGCCCCGA GGGCTACCAC GACCCCACCT GCCTGTCTGA GGTCAACGAG
    TGCAGCAGCA ACCCCTGCAT CCATGGGGCC TGCCGCGACA GCCTCAACGG GTACAAGTGT
    GACTGTGACC CCGGGTGGAG TGGGGCCAAC TGTGACGTCA ACAACGACGA GTGTGAGTCG
    AACCCCTGCA TCAACGGGGG CACCTGCAAG GACATGACCA GCGGCTACGT GTGCGCCTGC
    CGCGAGGGCT TCAGTGGGCC CAACTGCCAG ACCAACATCA ACGAGTGCGC ATCCAACCCA
    TGTCTGAACC AGGGCACGTG TATCGATGAC GTGGCGGGGT ACAAATGCAA CTGCCTCTTG
    CCCTACACAG GGGCCACGTG TGAGGTGGTG CTGGCCCCAT GTGCCCCCGG GCCCTGCAGA
    AACGGCGGTG AGTGTCGGGA GTCAGAGGAC TATGAGAGCT TCTCCTGTGC CTGCCCCGCG
    GGCTGGCAGG GGCAGACCTG CGAGATCGAC ATCAACGAGT GCGTGAAGAG CCCGTGCCGC
    GCGGGCGCCT CCTGCCAGAA CACCAACGGC AGCTACCGCT GCCACTGCCA GGCGGGCTAC
    ACGGGGCGCA ACTGCGAGAC GGACATCGAC GACTGCCGGC CCAACCCGTG CCACAATGGG
    GGCTCCTGCA CAGATGGCAT CAACACGGCC TTCTGCGACT GCCTGCCCGG CTTCCAGGGC
    GCCTTCTGCG AGGAGGACAT CAACGAGTGC GCCAGCAGCC CCTGCCGCAA CGGCGCCAAC
    TGCACCGACT GTGTGGACAG CTACACCTGC ACCTGCCCCA CGGGCTTCAG CGGCATCCAC
    TGCGAGAACA ACACGCCCGA CTGCACGGAG AGCTCCTGCT TCAACGGCGG TACCTGCGTG
    GATGGCATCA ACTCCTTCAC CTGCCTGTGT CCACCTGGCT TCACGGGCAG CTACTGCCAA
    CATGACGTCA ACGAGTGTGA CTCTCGGCCC TGCCTGCACG GCGGCACCTG CCACGACAGC
    TACGGCACCT ACACGTGCAC CTGCCCGCAG GGCTACACAG GCCTCAACTG CCAGACCCTC
    GTGCGCTGGT GCGACTCGTC CCCCTGCAAG AATGACGGCC GGTGCTGGCA GACCAACGCG
    CTCTACCGCT GTGAGTGCCA CAGCGGCTGG ACCGGCCTCT ACTGTGACGT GCCCAGCGTC
    TCCTGCGAGG TGGCCGCGCG GCAGCAAGGC GTCAACGTGA CCCACTTGTG CCGGAACGGG
    GGCCTCTGCA TGAATGCGGG CAACACACAC CGCTGCCACT GCCAGGCCGG CTACACGGGC
    AGCTACTGCG AGGAGCAGGT GGACGAGTGC TCGCCCAGCC CCTGCCAGAA CGGGGCCACC
    TGCACCGACT ACCCCGGCGG CTACTCCTGT GAGTGTGTGG CCGGCTACCA CGGGGTGAAC
    TGCTCCGAGG AGGTGAACGA GTGCCTGTCC CAGCCCTGCC GGAACGGAGG CACCTGCATC
    GACCTCACCA ACACCTACAA GTGTTCCTGC CCCCGCGGCA CACAGGGCGT GCACTGCGAG
    ATCAACGTGG ACGACTGCAA CCCCCCCATC GACCCCGTGT CCCGGGGCCC CAAGTGCTTC
    AACAACGGCA CCTGCGTGGA CCAGGTGGGC GGCTACAGCT GCAGCTGCCC GCCCGGCTTC
    GTGGGTGAGC GCTGCGAGGG GGATGTCAAC GAGTGCCTGT CCAACCCCTG CGACGCCCGT
    GGCACCCAGA ACTGCGTGCA GCACGTCAAC GCCTTCCACT GCGAGTGCCG CGCCGGCCAC
    ACCGGACGCC GCTGCGAGTC AGTCATCAAC GGCTGCAAAG ACAGGCCCTG TAAGAATGGG
    GGTAGCTGTG CAGTGGCCTC CAACACGGCC CGTGGGTTCA TCTGCAAGTG CCCAGCGGGC
    TTCGAGGGCG CCACGTGCGA GAACGACGCC CGCAGCTGCG GGAGCCTGCG GTGCCTGAAC
    GGCGGCACGT GCATCGCGGG CCCGCGCAGC CCCACCTGCC TGTGCCTGGG CCCCTTCACC
    GGCCCCGAGT GCCAGTTCCC GGCCAGCAGC CCCTGCGTGG GCGGCAACCC CTGCTACAAC
    CAGGGCGTCT GTGAGCCCAC CGCCGAGAGC CCCTTCTACC GCTGCCGCTG CCCCGCCAAG
    TTCAACGGGC TCCTGTGCCA CATCCTGGAC TACAGCTTCG GGGGCGGCGT GGGCCTCGAC
    ATCCCCCCGC CACAGATCGA GGAGACCTGC GAGCTCCCTG GCTGCCGCGA GGAGGCCGGC
    AACAAGGTCT GCAGCCTGCA GTGCAACAGC CACGCGTGCG GCTGGGACGG CGGCGACTGC
    TCCCTCGACT TCGACGACCC TTGGCAGAAC TGCACGCAGT CCCTGCAGTG CTGGAAGTAC
    TTCAGCAACG GCCGCTGCGA CAGCCAGTGC AACTCGGCCG GCTGCCTCTT CGACGGCTTC
    GACTGTCAGC GCGCAGAAGG CCAGTGCAAC CCCCTGTACG ACCAGTACTG CAAGGACCAC
    TTCAGGGACG GGCACTGCGA CCAGGGCTGC AACAGTGCGG AGTGCGAGTG GGACGGGCTG
    GACTGTGCGG AGCATGTGCC CGAGCGGCTG GCGGCCGGCA CGCTGGTGCT GGTGGTGCTC
    ATGCCGCCCG AGCAGCTGCG CAACCGCTCC CTGCACTTCC TGCGCGAGCT CAGCCGCCTG
    CTGCACACCA ATGTGGTCTT CAAGCGTGAT GCCAGTGGCC AGCAGATGAT CTTCCCCTAC
    TACGGCCGCG AGGAGGAGCT GCACAAGCAC CCCGTCAGGC GCTCCGTGGA CGGTGGGGCC
    CCACTGCTCC TGGGCGGCGC GGGCAGACGC CAGCGCAGGG AGCTGGACCC CATGGACATC
    CGCGGGTCCA TCGTCTACCT GGAGATCGAC AACCGGCAGT GCGTCCAGTC ATCCTCCCAG
    TGCTTCCAGA GCGCCACGGA CGTGGCCGCC TTCTTGGGCG CGCTGGCCTC GCTGGGCAGC
    CTTAACATCC CCTACAAGAT CGAGGCTGTG CAGAGTGAGA CGGTGGAGCC GCCCCCGCCG
    CCGCCGCTGC ACTTCATGTA TGTGGCTGTG GTGGCCTTCG TGTTGCTGTT CTTCGTGGGC
    TGCGGGGTGC TGCTGTCGCG GAAGCGCCGG CGGCAGCATG GCCAGCTCTG GTTCCCTGAG
    GGCTTCAAGG TGTCGGAGGC CAGCAAGAAG AAGCGGCGCG AGCCCCTCGG CGAGGACTCC
    GTGGGCCTCA AGCCCCTGAA GAACTCCTCG GACGGCGCCC TCATGGACGA CAACCAGAAC
    GAGTGGGGCG ACGAGGGCCT GGAGGCCAAG AAGTTCCGGT TCGAGGAGCC CGTGGTCCTC
    CCTGACCTGG ACGACCAGAC GGACCACCGG CAGTGGACCC AGCAGCACCT GGACGCCGCC
    GACCTGCGCG TGTCTGCCAT GGCCCCCACG CCTCCCCAGG GCGAGGCGGA TGCCGACTGC
    ATGGACGTGA ACGTCCGCGG GCCGGACGGC TTCACGCCCC TCATGATCGC CTCCTGCAGC
    GGAGGGGGCC TAGAGACTGG CAACAGCGAG GAGGAGGAGG ACGCGCCCGC GGTCATCTCG
    GACTTCATCT ACCAGGGCGC CAGCCTGCAC AACCAGACGG ACCGCACCGG CGAGACCGCC
    CTGCACCTGG CCGCCCGCTA CTCGCGCTCC GACGCGGCCA AGCGCCTGCT GGAGGCCAGC
    GCGGACGCCA ACATACAGGA CAACATGGGG CGCACCCCGC TGCACGCGGC CGTGTCTGCC
    GACGCACAGG GCGTTTTCCA GATCCTGATC CGGAACCGAG CCACCGACCT GGATGCCCGC
    ATGCACGACG GCACGACCCC GCTGATCCTC GCTGCCCGCC TGGCCGTGGA GGGCATGCTG
    GAGGACCTCA TCAACTCTCA CGCCGACGTC AACGCTGTGG ATGACCTGGG CAAGTCCGCC
    CTGCACTGGG CAGCCGCGGT GAACAACGTG GAGGCTGCTG TCGTGCTGCT GAAGAACGGG
    GCCAACAAGG ACATGCAGAA CAACAAGGAG GAGACGCCCT TGTTCCTGGC CGCCCGGGAG
    GGCAGCTACG AGACCGCCAA GGTGCTGCTG GACCATTTTG CCAACCGGGA CATCACAGAC
    CACATGGACC GCCTGCCTCG CGACATCGCG CAGGAGCGTA TGCACCACGA CATCGTGCGG
    CTGCTGGACG AGTACAGCCT GGTGCGCAGC CCCCCGCTGC ATGGCGCCAC CCTGGGCGGC
    ACGCCCACCC TGTCGCCCCC GCTCTGCTCG CCCAACGGCT ACCTGGGCAA CCTCAAGCCC
    CCCATGCAGG GCAAGAAGGC CCGCAAGCCC AGCACCAAGG GCCTGGCCTG CGGCGGCAAG
    GAGCCCAAGG ACCTCAAGGC TCGGAGGAAG AAGTCCCAAG ATGGCAAGGG CTGTCTGCTG
    GACAGCGGGA GCGTGATGTC GCCCGTGGAC TCCCTCGAGT CCCCCCACGG CTACCTGTCA
    GACGTGGCCT CCCCACCCCT CCTGCCCTCC CCTTTCCAGC CGTCTCCCTC CGTGCCCCTC
    AACCACCTGC CCGGGATGCC CGAGACCCAC CTGGGCGTCA GCCACCTGAG CGTGGCGGCC
    AAGCCCGAGA TGGCGGTGCT GAGCGGGGGC AGCCGGCTGG CCTTCGAGGC AGGGCCACCA
    CGCCTCTCCC ACCTGCCCGT GGCCTCCAGC ACCAGCACGA TCCTGGGTTC GGGCGGCAGT
    GGGGGCAGTG GGGCTGTGAA TTTCACCGTG GGTGGAGCTG CGGGCTTGAA TGGCCAGTGC
    GAGTGGCTGT CCCGGCTGCA GAACGGCCTG GTGCCCAACC AGTACAACCC GCTGCGAGGG
    GGCGTGACGC CGGGCACCCT GAGCACGCAG GCTGCTGGCC TCCAGCATGG CACGGTGGGC
    CCGCTGCACG CCCCCGCCCT GTCCCAGGTG ATGACCTACC AGGCCCTGCC CAGCACGCGG
    CTGGCCTCCC AGCCTCACCT GGTGCAGCCG CAGCAGAACT TACAGATGCA GCCGCCGAGC
    ATGCCGCCGC AGCCAAACCT GCAGCCGCAC CTCGGCGTAA GCTCGGCCGC CAGCGGCCAC
    CTGGGCCGGA GCTTCCTGGG AGGGGAGCTG AGCCAGGCGG ACATGCAGCC GCTGGGGCCC
    GGCAACCTGG CGGCGCACAC CGTCCTGCCG CAGGACGGCC AGGTCCTGCC CACATCGCTG
    CCGTCCACGC TGGCCCCGCC CACGATGGCC CCGCCCATGA CCACCGCCCA GTTCCTGACG
    CCCCCCTCCC AGCACAGCTA CTCGTCCTCC CCCGTGGACA ACACCCCCAG CCACCAGCTA
    CAGGTGCCCG AGCACCCCTT CCTCACCCCG TCCCCGGAGT CCCCCGACCA GTGGTCCAGC
    TCCTCGCCAC ATTCCAACAT CTCGGATTGG TCCGAGGGCA TCTCCAGCCC GCCCACCAGC
    GTGCCGTCCC AGATCGCCCA CGTCCCGGAG GCATTCAAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855411.1
    CDS263..7864
    Translation

    Target ORF information:

    RefSeq Version XM_024999643.1
    Organism Bos taurus(cattle)
    Definition Bos taurus notch 1 (NOTCH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999643.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGCCGCCGC TCCTGGCGCC CCTGCTTTGC CTGGCGTTGC TGCCTGCGCT CGCCGCACGA 
    GGCCTGCGCT GCTCCCAGCC CGGTGAGACC TGCTTGAACG GTGGGAAGTG TGAAGTGTTC
    CCCAACGGCA CCGAGGCCTG CATCTGCGGC GGGGCCTTCG CGGGCCAGCA ATGCCAGGCC
    CCCAACCCCT GCCTCAGCGC CCCCTGCAAG AACGGCGGGA CGTGCCACAC GACGGAGCGA
    GAAGGCCTGG TGGACTATGT CTGCGGCTGC CGCCTGGGCT TCTCGGGGCC ACTGTGCCTG
    ACGCCCCGCG ACCACGCTTG CCTCGCCAGC CCCTGCCTCA ACGGTGGCAC CTGTGATCTG
    CTCACGCTCA CTGAGTACAA GTGCCTGTGC ACCCCGGGCT GGTCAGGGAA GACGTGCCAG
    CAGGCCGACC CCTGCGCCTC CAACCCCTGT GCCAACGGTG GCCAGTGCCT GCCCTTTGAG
    GCGTCGTATA TCTGCCACTG CCCGCCCGGC TTCCACGGCC CCACGTGCAG GCAGGACGTC
    AACGAGTGCA GCCAGAGCCC CGGGCTCTGC CACCACGGCG GCACCTGCCT CAACGAGGTC
    GGCTCCTACC GCTGCGTCTG CCGCCCCACC CACACCGGCC CCCACTGCGA GCTGCCCTAC
    GTGCCCTGCA GTCCCTCCCC CTGCCAGAAC GGGGGCACCT GCCGCCCCAC GGGGGACACC
    ACCCACGAGT GCGCCTGCCT GCCAGGCTTC ACTGGCCAGA ACTGTGAGGA AAACATTGAC
    GACTGTCCGG GGAACAGCTG CAAGAACGGA GGCGCCTGTG TGGACGGCGT GAACACCTAC
    AACTGCCGCT GCCCCCCGGA GTGGACAGGT CAGTACTGCA CAGAGGACGT GGACGAGTGC
    CAGCTCATGC CCAACGCCTG CCAGAACGGC GGGACCTGCC ACAACACCCA CGGCGGCTAC
    AACTGCGTGT GCGTCAACGG CTGGACGGGC GAGGACTGCA GCGAGAACAT CGACGACTGC
    GCCAGCGCCT CCTGCTTCCA GGGCGCCACC TGCCACGACC GCGTCGCCTC CTTCTACTGC
    GAGTGTCCCC ACGGCCGCAC GGGTCTGCTG TGCCACCTGA ACGATGCCTG CATCAGCAAC
    CCCTGCAACG AGGGCTCCAA CTGCGACACC AACCCCGTCA ACGGCAAGGC CATCTGCACC
    TGCCCCTCGG GGTACACGGG GCCGGCCTGC AGCCAGGATG TGGACGAGTG CTCGCTGGGC
    GCCAACCCCT GTGAGCACGC GGGCAAGTGC ATCAACACGC TGGGCTCGTT CGAGTGCCAG
    TGTCTGCAGG GCTACACCGG CCCGCGCTGT GAGATCGACG TCAACGAGTG TGTATCGAAC
    CCGTGTCAGA ACGACGCCAC TTGTCTGGAT CAGATCGGGG AGTTCCAGTG TATCTGCATG
    CCTGGCTACG AGGGCTTGCA CTGCGAGGTG AACACGGACG AGTGTGCCAG CAGCCCCTGC
    CTGCAGAACG GCCGCTGCCT GGACAAGATC AACGAGTTCG TGTGCGAGTG CCCCACTGGC
    TTCACCGGGC ACTTGTGCCA GTATGACGTG GACGAGTGTG CAAGCACACC CTGCAAGAAC
    GGCGCTAAGT GCCTGGACGG GCCCAACACC TACACCTGCG TGTGCACAGA AGGCTACACG
    GGGCCCCACT GCGAGGTGGA CATCGACGAG TGTGACCCCG ACCCCTGCCA CTACGGGTCC
    TGCAAGGACG GCGTGGCCAC CTTCACCTGC CTGTGCCAGC CGGGCTACAC GGGCCACCAC
    TGTGAGAGCA ACATTAACGA GTGCCACAGC CAGCCCTGCC GCCACGGGGG CACCTGCCAG
    GACCGCGACA ACGCCTATCT CTGCTTCTGC CTCAAGGGGA CCACAGGACC CAACTGCGAG
    ATCAACCTGG ATGACTGCGC AAGCAACCCT TGCGACTCCG GCACGTGTCT GGACAAGATT
    GACGGCTACG AGTGTGCCTG TGAGCCAGGC TACACAGGGA GTATGTGTAA CATCAACATT
    GACGAGTGTG CGGACAGCCC TTGCCACAAC GGGGGCACCT GCGAGGATGG CATCAACGGC
    TTCACCTGTC GCTGCCCCGA GGGCTACCAC GACCCCACCT GCCTGTCTGA GGTCAACGAG
    TGCAGCAGCA ACCCCTGCAT CCATGGGGCC TGCCGCGACA GCCTCAACGG GTACAAGTGT
    GACTGTGACC CCGGGTGGAG TGGGGCCAAC TGTGACGTCA ACAACGACGA GTGTGAGTCG
    AACCCCTGCA TCAACGGGGG CACCTGCAAG GACATGACCA GCGGCTACGT GTGCGCCTGC
    CGCGAGGGCT TCAGTGGGCC CAACTGCCAG ACCAACATCA ACGAGTGCGC ATCCAACCCA
    TGTCTGAACC AGGGCACGTG TATCGATGAC GTGGCGGGGT ACAAATGCAA CTGCCTCTTG
    CCCTACACAG GGGCCACGTG TGAGGTGGTG CTGGCCCCAT GTGCCCCCGG GCCCTGCAGA
    AACGGCGGTG AGTGTCGGGA GTCAGAGGAC TATGAGAGCT TCTCCTGTGC CTGCCCCGCG
    GGCTGGCAGG GGCAGACCTG CGAGATCGAC ATCAACGAGT GCGTGAAGAG CCCGTGCCGC
    GCGGGCGCCT CCTGCCAGAA CACCAACGGC AGCTACCGCT GCCACTGCCA GGCGGGCTAC
    ACGGGGCGCA ACTGCGAGAC GGACATCGAC GACTGCCGGC CCAACCCGTG CCACAATGGG
    GGCTCCTGCA CAGATGGCAT CAACACGGCC TTCTGCGACT GCCTGCCCGG CTTCCAGGGC
    GCCTTCTGCG AGGAGGACAT CAACGAGTGC GCCAGCAGCC CCTGCCGCAA CGGCGCCAAC
    TGCACCGACT GTGTGGACAG CTACACCTGC ACCTGCCCCA CGGGCTTCAG CGGCATCCAC
    TGCGAGAACA ACACGCCCGA CTGCACGGAG AGCTCCTGCT TCAACGGCGG TACCTGCGTG
    GATGGCATCA ACTCCTTCAC CTGCCTGTGT CCACCTGGCT TCACGGGCAG CTACTGCCAA
    CATGACGTCA ACGAGTGTGA CTCTCGGCCC TGCCTGCACG GCGGCACCTG CCACGACAGC
    TACGGCACCT ACACGTGCAC CTGCCCGCAG GGCTACACAG GCCTCAACTG CCAGACCCTC
    GTGCGCTGGT GCGACTCGTC CCCCTGCAAG AATGACGGCC GGTGCTGGCA GACCAACGCG
    CTCTACCGCT GTGAGTGCCA CAGCGGCTGG ACCGGCCTCT ACTGTGACGT GCCCAGCGTC
    TCCTGCGAGG TGGCCGCGCG GCAGCAAGGC GTCAACGTGA CCCACTTGTG CCGGAACGGG
    GGCCTCTGCA TGAATGCGGG CAACACACAC CGCTGCCACT GCCAGGCCGG CTACACGGGC
    AGCTACTGCG AGGAGCAGGT GGACGAGTGC TCGCCCAGCC CCTGCCAGAA CGGGGCCACC
    TGCACCGACT ACCCCGGCGG CTACTCCTGT GAGTGTGTGG CCGGCTACCA CGGGGTGAAC
    TGCTCCGAGG AGGTGAACGA GTGCCTGTCC CAGCCCTGCC GGAACGGAGG CACCTGCATC
    GACCTCACCA ACACCTACAA GTGTTCCTGC CCCCGCGGCA CACAGGGCGT GCACTGCGAG
    ATCAACGTGG ACGACTGCAA CCCCCCCATC GACCCCGTGT CCCGGGGCCC CAAGTGCTTC
    AACAACGGCA CCTGCGTGGA CCAGGTGGGC GGCTACAGCT GCAGCTGCCC GCCCGGCTTC
    GTGGGTGAGC GCTGCGAGGG GGATGTCAAC GAGTGCCTGT CCAACCCCTG CGACGCCCGT
    GGCACCCAGA ACTGCGTGCA GCACGTCAAC GCCTTCCACT GCGAGTGCCG CGCCGGCCAC
    ACCGGACGCC GCTGCGAGTC AGTCATCAAC GGCTGCAAAG ACAGGCCCTG TAAGAATGGG
    GGTAGCTGTG CAGTGGCCTC CAACACGGCC CGTGGGTTCA TCTGCAAGTG CCCAGCGGGC
    TTCGAGGGCG CCACGTGCGA GAACGACGCC CGCAGCTGCG GGAGCCTGCG GTGCCTGAAC
    GGCGGCACGT GCATCGCGGG CCCGCGCAGC CCCACCTGCC TGTGCCTGGG CCCCTTCACC
    GGCCCCGAGT GCCAGTTCCC GGCCAGCAGC CCCTGCGTGG GCGGCAACCC CTGCTACAAC
    CAGGGCGTCT GTGAGCCCAC CGCCGAGAGC CCCTTCTACC GCTGCCGCTG CCCCGCCAAG
    TTCAACGGGC TCCTGTGCCA CATCCTGGAC TACAGCTTCG GGGGCGGCGT GGGCCTCGAC
    ATCCCCCCGC CACAGATCGA GGAGACCTGC GAGCTCCCTG GCTGCCGCGA GGAGGCCGGC
    AACAAGGTCT GCAGCCTGCA GTGCAACAGC CACGCGTGCG GCTGGGACGG CGGCGACTGC
    TCCCTCGACT TCGACGACCC TTGGCAGAAC TGCACGCAGT CCCTGCAGTG CTGGAAGTAC
    TTCAGCAACG GCCGCTGCGA CAGCCAGTGC AACTCGGCCG GCTGCCTCTT CGACGGCTTC
    GACTGTCAGC GCGCAGAAGG CCAGTGCAAC CCCCTGTACG ACCAGTACTG CAAGGACCAC
    TTCAGGGACG GGCACTGCGA CCAGGGCTGC AACAGTGCGG AGTGCGAGTG GGACGGGCTG
    GACTGTGCGG AGCATGTGCC CGAGCGGCTG GCGGCCGGCA CGCTGGTGCT GGTGGTGCTC
    ATGCCGCCCG AGCAGCTGCG CAACCGCTCC CTGCACTTCC TGCGCGAGCT CAGCCGCCTG
    CTGCACACCA ATGTGGTCTT CAAGCGTGAT GCCAGTGGCC AGCAGATGAT CTTCCCCTAC
    TACGGCCGCG AGGAGGAGCT GCACAAGCAC CCCGTCAGGC GCTCCGTGGA CGGTGGGGCC
    CCACTGCTCC TGGGCGGCGC GGGCAGACGC CAGCGCAGGG AGCTGGACCC CATGGACATC
    CGCGGGTCCA TCGTCTACCT GGAGATCGAC AACCGGCAGT GCGTCCAGTC ATCCTCCCAG
    TGCTTCCAGA GCGCCACGGA CGTGGCCGCC TTCTTGGGCG CGCTGGCCTC GCTGGGCAGC
    CTTAACATCC CCTACAAGAT CGAGGCTGTG CAGAGTGAGA CGGTGGAGCC GCCCCCGCCG
    CCGCCGCTGC ACTTCATGTA TGTGGCTGTG GTGGCCTTCG TGTTGCTGTT CTTCGTGGGC
    TGCGGGGTGC TGCTGTCGCG GAAGCGCCGG CGGCAGCATG GCCAGCTCTG GTTCCCTGAG
    GGCTTCAAGG TGTCGGAGGC CAGCAAGAAG AAGCGGCGCG AGCCCCTCGG CGAGGACTCC
    GTGGGCCTCA AGCCCCTGAA GAACTCCTCG GACGGCGCCC TCATGGACGA CAACCAGAAC
    GAGTGGGGCG ACGAGGGCCT GGAGGCCAAG AAGTTCCGGT TCGAGGAGCC CGTGGTCCTC
    CCTGACCTGG ACGACCAGAC GGACCACCGG CAGTGGACCC AGCAGCACCT GGACGCCGCC
    GACCTGCGCG TGTCTGCCAT GGCCCCCACG CCTCCCCAGG GCGAGGCGGA TGCCGACTGC
    ATGGACGTGA ACGTCCGCGG GCCGGACGGC TTCACGCCCC TCATGATCGC CTCCTGCAGC
    GGAGGGGGCC TAGAGACTGG CAACAGCGAG GAGGAGGAGG ACGCGCCCGC GGTCATCTCG
    GACTTCATCT ACCAGGGCGC CAGCCTGCAC AACCAGACGG ACCGCACCGG CGAGACCGCC
    CTGCACCTGG CCGCCCGCTA CTCGCGCTCC GACGCGGCCA AGCGCCTGCT GGAGGCCAGC
    GCGGACGCCA ACATACAGGA CAACATGGGG CGCACCCCGC TGCACGCGGC CGTGTCTGCC
    GACGCACAGG GCGTTTTCCA GATCCTGATC CGGAACCGAG CCACCGACCT GGATGCCCGC
    ATGCACGACG GCACGACCCC GCTGATCCTC GCTGCCCGCC TGGCCGTGGA GGGCATGCTG
    GAGGACCTCA TCAACTCTCA CGCCGACGTC AACGCTGTGG ATGACCTGGG CAAGTCCGCC
    CTGCACTGGG CAGCCGCGGT GAACAACGTG GAGGCTGCTG TCGTGCTGCT GAAGAACGGG
    GCCAACAAGG ACATGCAGAA CAACAAGGAG GAGACGCCCT TGTTCCTGGC CGCCCGGGAG
    GGCAGCTACG AGACCGCCAA GGTGCTGCTG GACCATTTTG CCAACCGGGA CATCACAGAC
    CACATGGACC GCCTGCCTCG CGACATCGCG CAGGAGCGTA TGCACCACGA CATCGTGCGG
    CTGCTGGACG AGTACAGCCT GGTGCGCAGC CCCCCGCTGC ATGGCGCCAC CCTGGGCGGC
    ACGCCCACCC TGTCGCCCCC GCTCTGCTCG CCCAACGGCT ACCTGGGCAA CCTCAAGCCC
    CCCATGCAGG GCAAGAAGGC CCGCAAGCCC AGCACCAAGG GCCTGGCCTG CGGCGGCAAG
    GAGCCCAAGG ACCTCAAGGC TCGGAGGAAG AAGTCCCAAG ATGGCAAGGG CTGTCTGCTG
    GACAGCGGGA GCGTGATGTC GCCCGTGGAC TCCCTCGAGT CCCCCCACGG CTACCTGTCA
    GACGTGGCCT CCCCACCCCT CCTGCCCTCC CCTTTCCAGC CGTCTCCCTC CGTGCCCCTC
    AACCACCTGC CCGGGATGCC CGAGACCCAC CTGGGCGTCA GCCACCTGAG CGTGGCGGCC
    AAGCCCGAGA TGGCGGTGCT GAGCGGGGGC AGCCGGCTGG CCTTCGAGGC AGGGCCACCA
    CGCCTCTCCC ACCTGCCCGT GGCCTCCAGC ACCAGCACGA TCCTGGGTTC GGGCGGCAGT
    GGGGGCAGTG GGGCTGTGAA TTTCACCGTG GGTGGAGCTG CGGGCTTGAA TGGCCAGTGC
    GAGTGGCTGT CCCGGCTGCA GAACGGCCTG GTGCCCAACC AGTACAACCC GCTGCGAGGG
    GGCGTGACGC CGGGCACCCT GAGCACGCAG GCTGCTGGCC TCCAGCATGG CACGGTGGGC
    CCGCTGCACG CCCCCGCCCT GTCCCAGGTG ATGACCTACC AGGCCCTGCC CAGCACGCGG
    CTGGCCTCCC AGCCTCACCT GGTGCAGCCG CAGCAGAACT TACAGATGCA GCCGCCGAGC
    ATGCCGCCGC AGCCAAACCT GCAGCCGCAC CTCGGCGTAA GCTCGGCCGC CAGCGGCCAC
    CTGGGCCGGA GCTTCCTGGG AGGGGAGCTG AGCCAGGCGG ACATGCAGCC GCTGGGGCCC
    GGCAACCTGG CGGCGCACAC CGTCCTGCCG CAGGACGGCC AGGTCCTGCC CACATCGCTG
    CCGTCCACGC TGGCCCCGCC CACGATGGCC CCGCCCATGA CCACCGCCCA GTTCCTGACG
    CCCCCCTCCC AGCACAGCTA CTCGTCCTCC CCCGTGGACA ACACCCCCAG CCACCAGCTA
    CAGGTGCCCG AGCACCCCTT CCTCACCCCG TCCCCGGAGT CCCCCGACCA GTGGTCCAGC
    TCCTCGCCAC ATTCCAACAT CTCGGATTGG TCCGAGGGCA TCTCCAGCCC GCCCACCAGC
    GTGCCGTCCC AGATCGCCCA CGTCCCGGAG GCATTCAAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220990
    Clone ID Related Accession (Same CDS sequence) XM_024999644.1
    Accession Version XM_024999644.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neurogenic locus notch homolog protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGCAGGCCC TGCCCACAGG TCAGTACTGC ACAGAGGACG TGGACGAGTG CCAGCTCATG 
    CCCAACGCCT GCCAGAACGG CGGGACCTGC CACAACACCC ACGGCGGCTA CAACTGCGTG
    TGCGTCAACG GCTGGACGGG CGAGGACTGC AGCGAGAACA TCGACGACTG CGCCAGCGCC
    TCCTGCTTCC AGGGCGCCAC CTGCCACGAC CGCGTCGCCT CCTTCTACTG CGAGTGTCCC
    CACGGCCGCA CGGGTCTGCT GTGCCACCTG AACGATGCCT GCATCAGCAA CCCCTGCAAC
    GAGGGCTCCA ACTGCGACAC CAACCCCGTC AACGGCAAGG CCATCTGCAC CTGCCCCTCG
    GGGTACACGG GGCCGGCCTG CAGCCAGGAT GTGGACGAGT GCTCGCTGGG CGCCAACCCC
    TGTGAGCACG CGGGCAAGTG CATCAACACG CTGGGCTCGT TCGAGTGCCA GTGTCTGCAG
    GGCTACACCG GCCCGCGCTG TGAGATCGAC GTCAACGAGT GTGTATCGAA CCCGTGTCAG
    AACGACGCCA CTTGTCTGGA TCAGATCGGG GAGTTCCAGT GTATCTGCAT GCCTGGCTAC
    GAGGGCTTGC ACTGCGAGGT GAACACGGAC GAGTGTGCCA GCAGCCCCTG CCTGCAGAAC
    GGCCGCTGCC TGGACAAGAT CAACGAGTTC GTGTGCGAGT GCCCCACTGG CTTCACCGGG
    CACTTGTGCC AGTATGACGT GGACGAGTGT GCAAGCACAC CCTGCAAGAA CGGCGCTAAG
    TGCCTGGACG GGCCCAACAC CTACACCTGC GTGTGCACAG AAGGCTACAC GGGGCCCCAC
    TGCGAGGTGG ACATCGACGA GTGTGACCCC GACCCCTGCC ACTACGGGTC CTGCAAGGAC
    GGCGTGGCCA CCTTCACCTG CCTGTGCCAG CCGGGCTACA CGGGCCACCA CTGTGAGAGC
    AACATTAACG AGTGCCACAG CCAGCCCTGC CGCCACGGGG GCACCTGCCA GGACCGCGAC
    AACGCCTATC TCTGCTTCTG CCTCAAGGGG ACCACAGGAC CCAACTGCGA GATCAACCTG
    GATGACTGCG CAAGCAACCC TTGCGACTCC GGCACGTGTC TGGACAAGAT TGACGGCTAC
    GAGTGTGCCT GTGAGCCAGG CTACACAGGG AGTATGTGTA ACATCAACAT TGACGAGTGT
    GCGGACAGCC CTTGCCACAA CGGGGGCACC TGCGAGGATG GCATCAACGG CTTCACCTGT
    CGCTGCCCCG AGGGCTACCA CGACCCCACC TGCCTGTCTG AGGTCAACGA GTGCAGCAGC
    AACCCCTGCA TCCATGGGGC CTGCCGCGAC AGCCTCAACG GGTACAAGTG TGACTGTGAC
    CCCGGGTGGA GTGGGGCCAA CTGTGACGTC AACAACGACG AGTGTGAGTC GAACCCCTGC
    ATCAACGGGG GCACCTGCAA GGACATGACC AGCGGCTACG TGTGCGCCTG CCGCGAGGGC
    TTCAGTGGGC CCAACTGCCA GACCAACATC AACGAGTGCG CATCCAACCC ATGTCTGAAC
    CAGGGCACGT GTATCGATGA CGTGGCGGGG TACAAATGCA ACTGCCTCTT GCCCTACACA
    GGGGCCACGT GTGAGGTGGT GCTGGCCCCA TGTGCCCCCG GGCCCTGCAG AAACGGCGGT
    GAGTGTCGGG AGTCAGAGGA CTATGAGAGC TTCTCCTGTG CCTGCCCCGC GGGCTGGCAG
    GGGCAGACCT GCGAGATCGA CATCAACGAG TGCGTGAAGA GCCCGTGCCG CGCGGGCGCC
    TCCTGCCAGA ACACCAACGG CAGCTACCGC TGCCACTGCC AGGCGGGCTA CACGGGGCGC
    AACTGCGAGA CGGACATCGA CGACTGCCGG CCCAACCCGT GCCACAATGG GGGCTCCTGC
    ACAGATGGCA TCAACACGGC CTTCTGCGAC TGCCTGCCCG GCTTCCAGGG CGCCTTCTGC
    GAGGAGGACA TCAACGAGTG CGCCAGCAGC CCCTGCCGCA ACGGCGCCAA CTGCACCGAC
    TGTGTGGACA GCTACACCTG CACCTGCCCC ACGGGCTTCA GCGGCATCCA CTGCGAGAAC
    AACACGCCCG ACTGCACGGA GAGCTCCTGC TTCAACGGCG GTACCTGCGT GGATGGCATC
    AACTCCTTCA CCTGCCTGTG TCCACCTGGC TTCACGGGCA GCTACTGCCA ACATGACGTC
    AACGAGTGTG ACTCTCGGCC CTGCCTGCAC GGCGGCACCT GCCACGACAG CTACGGCACC
    TACACGTGCA CCTGCCCGCA GGGCTACACA GGCCTCAACT GCCAGACCCT CGTGCGCTGG
    TGCGACTCGT CCCCCTGCAA GAATGACGGC CGGTGCTGGC AGACCAACGC GCTCTACCGC
    TGTGAGTGCC ACAGCGGCTG GACCGGCCTC TACTGTGACG TGCCCAGCGT CTCCTGCGAG
    GTGGCCGCGC GGCAGCAAGG CGTCAACGTG ACCCACTTGT GCCGGAACGG GGGCCTCTGC
    ATGAATGCGG GCAACACACA CCGCTGCCAC TGCCAGGCCG GCTACACGGG CAGCTACTGC
    GAGGAGCAGG TGGACGAGTG CTCGCCCAGC CCCTGCCAGA ACGGGGCCAC CTGCACCGAC
    TACCCCGGCG GCTACTCCTG TGAGTGTGTG GCCGGCTACC ACGGGGTGAA CTGCTCCGAG
    GAGGTGAACG AGTGCCTGTC CCAGCCCTGC CGGAACGGAG GCACCTGCAT CGACCTCACC
    AACACCTACA AGTGTTCCTG CCCCCGCGGC ACACAGGGCG TGCACTGCGA GATCAACGTG
    GACGACTGCA ACCCCCCCAT CGACCCCGTG TCCCGGGGCC CCAAGTGCTT CAACAACGGC
    ACCTGCGTGG ACCAGGTGGG CGGCTACAGC TGCAGCTGCC CGCCCGGCTT CGTGGGTGAG
    CGCTGCGAGG GGGATGTCAA CGAGTGCCTG TCCAACCCCT GCGACGCCCG TGGCACCCAG
    AACTGCGTGC AGCACGTCAA CGCCTTCCAC TGCGAGTGCC GCGCCGGCCA CACCGGACGC
    CGCTGCGAGT CAGTCATCAA CGGCTGCAAA GACAGGCCCT GTAAGAATGG GGGTAGCTGT
    GCAGTGGCCT CCAACACGGC CCGTGGGTTC ATCTGCAAGT GCCCAGCGGG CTTCGAGGGC
    GCCACGTGCG AGAACGACGC CCGCAGCTGC GGGAGCCTGC GGTGCCTGAA CGGCGGCACG
    TGCATCGCGG GCCCGCGCAG CCCCACCTGC CTGTGCCTGG GCCCCTTCAC CGGCCCCGAG
    TGCCAGTTCC CGGCCAGCAG CCCCTGCGTG GGCGGCAACC CCTGCTACAA CCAGGGCGTC
    TGTGAGCCCA CCGCCGAGAG CCCCTTCTAC CGCTGCCGCT GCCCCGCCAA GTTCAACGGG
    CTCCTGTGCC ACATCCTGGA CTACAGCTTC GGGGGCGGCG TGGGCCTCGA CATCCCCCCG
    CCACAGATCG AGGAGACCTG CGAGCTCCCT GGCTGCCGCG AGGAGGCCGG CAACAAGGTC
    TGCAGCCTGC AGTGCAACAG CCACGCGTGC GGCTGGGACG GCGGCGACTG CTCCCTCGAC
    TTCGACGACC CTTGGCAGAA CTGCACGCAG TCCCTGCAGT GCTGGAAGTA CTTCAGCAAC
    GGCCGCTGCG ACAGCCAGTG CAACTCGGCC GGCTGCCTCT TCGACGGCTT CGACTGTCAG
    CGCGCAGAAG GCCAGTGCAA CCCCCTGTAC GACCAGTACT GCAAGGACCA CTTCAGGGAC
    GGGCACTGCG ACCAGGGCTG CAACAGTGCG GAGTGCGAGT GGGACGGGCT GGACTGTGCG
    GAGCATGTGC CCGAGCGGCT GGCGGCCGGC ACGCTGGTGC TGGTGGTGCT CATGCCGCCC
    GAGCAGCTGC GCAACCGCTC CCTGCACTTC CTGCGCGAGC TCAGCCGCCT GCTGCACACC
    AATGTGGTCT TCAAGCGTGA TGCCAGTGGC CAGCAGATGA TCTTCCCCTA CTACGGCCGC
    GAGGAGGAGC TGCACAAGCA CCCCGTCAGG CGCTCCGTGG ACGGTGGGGC CCCACTGCTC
    CTGGGCGGCG CGGGCAGACG CCAGCGCAGG GAGCTGGACC CCATGGACAT CCGCGGGTCC
    ATCGTCTACC TGGAGATCGA CAACCGGCAG TGCGTCCAGT CATCCTCCCA GTGCTTCCAG
    AGCGCCACGG ACGTGGCCGC CTTCTTGGGC GCGCTGGCCT CGCTGGGCAG CCTTAACATC
    CCCTACAAGA TCGAGGCTGT GCAGAGTGAG ACGGTGGAGC CGCCCCCGCC GCCGCCGCTG
    CACTTCATGT ATGTGGCTGT GGTGGCCTTC GTGTTGCTGT TCTTCGTGGG CTGCGGGGTG
    CTGCTGTCGC GGAAGCGCCG GCGGCAGCAT GGCCAGCTCT GGTTCCCTGA GGGCTTCAAG
    GTGTCGGAGG CCAGCAAGAA GAAGCGGCGC GAGCCCCTCG GCGAGGACTC CGTGGGCCTC
    AAGCCCCTGA AGAACTCCTC GGACGGCGCC CTCATGGACG ACAACCAGAA CGAGTGGGGC
    GACGAGGGCC TGGAGGCCAA GAAGTTCCGG TTCGAGGAGC CCGTGGTCCT CCCTGACCTG
    GACGACCAGA CGGACCACCG GCAGTGGACC CAGCAGCACC TGGACGCCGC CGACCTGCGC
    GTGTCTGCCA TGGCCCCCAC GCCTCCCCAG GGCGAGGCGG ATGCCGACTG CATGGACGTG
    AACGTCCGCG GGCCGGACGG CTTCACGCCC CTCATGATCG CCTCCTGCAG CGGAGGGGGC
    CTAGAGACTG GCAACAGCGA GGAGGAGGAG GACGCGCCCG CGGTCATCTC GGACTTCATC
    TACCAGGGCG CCAGCCTGCA CAACCAGACG GACCGCACCG GCGAGACCGC CCTGCACCTG
    GCCGCCCGCT ACTCGCGCTC CGACGCGGCC AAGCGCCTGC TGGAGGCCAG CGCGGACGCC
    AACATACAGG ACAACATGGG GCGCACCCCG CTGCACGCGG CCGTGTCTGC CGACGCACAG
    GGCGTTTTCC AGATCCTGAT CCGGAACCGA GCCACCGACC TGGATGCCCG CATGCACGAC
    GGCACGACCC CGCTGATCCT CGCTGCCCGC CTGGCCGTGG AGGGCATGCT GGAGGACCTC
    ATCAACTCTC ACGCCGACGT CAACGCTGTG GATGACCTGG GCAAGTCCGC CCTGCACTGG
    GCAGCCGCGG TGAACAACGT GGAGGCTGCT GTCGTGCTGC TGAAGAACGG GGCCAACAAG
    GACATGCAGA ACAACAAGGA GGAGACGCCC TTGTTCCTGG CCGCCCGGGA GGGCAGCTAC
    GAGACCGCCA AGGTGCTGCT GGACCATTTT GCCAACCGGG ACATCACAGA CCACATGGAC
    CGCCTGCCTC GCGACATCGC GCAGGAGCGT ATGCACCACG ACATCGTGCG GCTGCTGGAC
    GAGTACAGCC TGGTGCGCAG CCCCCCGCTG CATGGCGCCA CCCTGGGCGG CACGCCCACC
    CTGTCGCCCC CGCTCTGCTC GCCCAACGGC TACCTGGGCA ACCTCAAGCC CCCCATGCAG
    GGCAAGAAGG CCCGCAAGCC CAGCACCAAG GGCCTGGCCT GCGGCGGCAA GGAGCCCAAG
    GACCTCAAGG CTCGGAGGAA GAAGTCCCAA GATGGCAAGG GCTGTCTGCT GGACAGCGGG
    AGCGTGATGT CGCCCGTGGA CTCCCTCGAG TCCCCCCACG GCTACCTGTC AGACGTGGCC
    TCCCCACCCC TCCTGCCCTC CCCTTTCCAG CCGTCTCCCT CCGTGCCCCT CAACCACCTG
    CCCGGGATGC CCGAGACCCA CCTGGGCGTC AGCCACCTGA GCGTGGCGGC CAAGCCCGAG
    ATGGCGGTGC TGAGCGGGGG CAGCCGGCTG GCCTTCGAGG CAGGGCCACC ACGCCTCTCC
    CACCTGCCCG TGGCCTCCAG CACCAGCACG ATCCTGGGTT CGGGCGGCAG TGGGGGCAGT
    GGGGCTGTGA ATTTCACCGT GGGTGGAGCT GCGGGCTTGA ATGGCCAGTG CGAGTGGCTG
    TCCCGGCTGC AGAACGGCCT GGTGCCCAAC CAGTACAACC CGCTGCGAGG GGGCGTGACG
    CCGGGCACCC TGAGCACGCA GGCTGCTGGC CTCCAGCATG GCACGGTGGG CCCGCTGCAC
    GCCCCCGCCC TGTCCCAGGT GATGACCTAC CAGGCCCTGC CCAGCACGCG GCTGGCCTCC
    CAGCCTCACC TGGTGCAGCC GCAGCAGAAC TTACAGATGC AGCCGCCGAG CATGCCGCCG
    CAGCCAAACC TGCAGCCGCA CCTCGGCGTA AGCTCGGCCG CCAGCGGCCA CCTGGGCCGG
    AGCTTCCTGG GAGGGGAGCT GAGCCAGGCG GACATGCAGC CGCTGGGGCC CGGCAACCTG
    GCGGCGCACA CCGTCCTGCC GCAGGACGGC CAGGTCCTGC CCACATCGCT GCCGTCCACG
    CTGGCCCCGC CCACGATGGC CCCGCCCATG ACCACCGCCC AGTTCCTGAC GCCCCCCTCC
    CAGCACAGCT ACTCGTCCTC CCCCGTGGAC AACACCCCCA GCCACCAGCT ACAGGTGCCC
    GAGCACCCCT TCCTCACCCC GTCCCCGGAG TCCCCCGACC AGTGGTCCAG CTCCTCGCCA
    CATTCCAACA TCTCGGATTG GTCCGAGGGC ATCTCCAGCC CGCCCACCAG CGTGCCGTCC
    CAGATCGCCC ACGTCCCGGA GGCATTCAAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855412.1
    CDS224..6976
    Translation

    Target ORF information:

    RefSeq Version XM_024999644.1
    Organism Bos taurus(cattle)
    Definition Bos taurus notch 1 (NOTCH1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999644.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGCAGGCCC TGCCCACAGG TCAGTACTGC ACAGAGGACG TGGACGAGTG CCAGCTCATG 
    CCCAACGCCT GCCAGAACGG CGGGACCTGC CACAACACCC ACGGCGGCTA CAACTGCGTG
    TGCGTCAACG GCTGGACGGG CGAGGACTGC AGCGAGAACA TCGACGACTG CGCCAGCGCC
    TCCTGCTTCC AGGGCGCCAC CTGCCACGAC CGCGTCGCCT CCTTCTACTG CGAGTGTCCC
    CACGGCCGCA CGGGTCTGCT GTGCCACCTG AACGATGCCT GCATCAGCAA CCCCTGCAAC
    GAGGGCTCCA ACTGCGACAC CAACCCCGTC AACGGCAAGG CCATCTGCAC CTGCCCCTCG
    GGGTACACGG GGCCGGCCTG CAGCCAGGAT GTGGACGAGT GCTCGCTGGG CGCCAACCCC
    TGTGAGCACG CGGGCAAGTG CATCAACACG CTGGGCTCGT TCGAGTGCCA GTGTCTGCAG
    GGCTACACCG GCCCGCGCTG TGAGATCGAC GTCAACGAGT GTGTATCGAA CCCGTGTCAG
    AACGACGCCA CTTGTCTGGA TCAGATCGGG GAGTTCCAGT GTATCTGCAT GCCTGGCTAC
    GAGGGCTTGC ACTGCGAGGT GAACACGGAC GAGTGTGCCA GCAGCCCCTG CCTGCAGAAC
    GGCCGCTGCC TGGACAAGAT CAACGAGTTC GTGTGCGAGT GCCCCACTGG CTTCACCGGG
    CACTTGTGCC AGTATGACGT GGACGAGTGT GCAAGCACAC CCTGCAAGAA CGGCGCTAAG
    TGCCTGGACG GGCCCAACAC CTACACCTGC GTGTGCACAG AAGGCTACAC GGGGCCCCAC
    TGCGAGGTGG ACATCGACGA GTGTGACCCC GACCCCTGCC ACTACGGGTC CTGCAAGGAC
    GGCGTGGCCA CCTTCACCTG CCTGTGCCAG CCGGGCTACA CGGGCCACCA CTGTGAGAGC
    AACATTAACG AGTGCCACAG CCAGCCCTGC CGCCACGGGG GCACCTGCCA GGACCGCGAC
    AACGCCTATC TCTGCTTCTG CCTCAAGGGG ACCACAGGAC CCAACTGCGA GATCAACCTG
    GATGACTGCG CAAGCAACCC TTGCGACTCC GGCACGTGTC TGGACAAGAT TGACGGCTAC
    GAGTGTGCCT GTGAGCCAGG CTACACAGGG AGTATGTGTA ACATCAACAT TGACGAGTGT
    GCGGACAGCC CTTGCCACAA CGGGGGCACC TGCGAGGATG GCATCAACGG CTTCACCTGT
    CGCTGCCCCG AGGGCTACCA CGACCCCACC TGCCTGTCTG AGGTCAACGA GTGCAGCAGC
    AACCCCTGCA TCCATGGGGC CTGCCGCGAC AGCCTCAACG GGTACAAGTG TGACTGTGAC
    CCCGGGTGGA GTGGGGCCAA CTGTGACGTC AACAACGACG AGTGTGAGTC GAACCCCTGC
    ATCAACGGGG GCACCTGCAA GGACATGACC AGCGGCTACG TGTGCGCCTG CCGCGAGGGC
    TTCAGTGGGC CCAACTGCCA GACCAACATC AACGAGTGCG CATCCAACCC ATGTCTGAAC
    CAGGGCACGT GTATCGATGA CGTGGCGGGG TACAAATGCA ACTGCCTCTT GCCCTACACA
    GGGGCCACGT GTGAGGTGGT GCTGGCCCCA TGTGCCCCCG GGCCCTGCAG AAACGGCGGT
    GAGTGTCGGG AGTCAGAGGA CTATGAGAGC TTCTCCTGTG CCTGCCCCGC GGGCTGGCAG
    GGGCAGACCT GCGAGATCGA CATCAACGAG TGCGTGAAGA GCCCGTGCCG CGCGGGCGCC
    TCCTGCCAGA ACACCAACGG CAGCTACCGC TGCCACTGCC AGGCGGGCTA CACGGGGCGC
    AACTGCGAGA CGGACATCGA CGACTGCCGG CCCAACCCGT GCCACAATGG GGGCTCCTGC
    ACAGATGGCA TCAACACGGC CTTCTGCGAC TGCCTGCCCG GCTTCCAGGG CGCCTTCTGC
    GAGGAGGACA TCAACGAGTG CGCCAGCAGC CCCTGCCGCA ACGGCGCCAA CTGCACCGAC
    TGTGTGGACA GCTACACCTG CACCTGCCCC ACGGGCTTCA GCGGCATCCA CTGCGAGAAC
    AACACGCCCG ACTGCACGGA GAGCTCCTGC TTCAACGGCG GTACCTGCGT GGATGGCATC
    AACTCCTTCA CCTGCCTGTG TCCACCTGGC TTCACGGGCA GCTACTGCCA ACATGACGTC
    AACGAGTGTG ACTCTCGGCC CTGCCTGCAC GGCGGCACCT GCCACGACAG CTACGGCACC
    TACACGTGCA CCTGCCCGCA GGGCTACACA GGCCTCAACT GCCAGACCCT CGTGCGCTGG
    TGCGACTCGT CCCCCTGCAA GAATGACGGC CGGTGCTGGC AGACCAACGC GCTCTACCGC
    TGTGAGTGCC ACAGCGGCTG GACCGGCCTC TACTGTGACG TGCCCAGCGT CTCCTGCGAG
    GTGGCCGCGC GGCAGCAAGG CGTCAACGTG ACCCACTTGT GCCGGAACGG GGGCCTCTGC
    ATGAATGCGG GCAACACACA CCGCTGCCAC TGCCAGGCCG GCTACACGGG CAGCTACTGC
    GAGGAGCAGG TGGACGAGTG CTCGCCCAGC CCCTGCCAGA ACGGGGCCAC CTGCACCGAC
    TACCCCGGCG GCTACTCCTG TGAGTGTGTG GCCGGCTACC ACGGGGTGAA CTGCTCCGAG
    GAGGTGAACG AGTGCCTGTC CCAGCCCTGC CGGAACGGAG GCACCTGCAT CGACCTCACC
    AACACCTACA AGTGTTCCTG CCCCCGCGGC ACACAGGGCG TGCACTGCGA GATCAACGTG
    GACGACTGCA ACCCCCCCAT CGACCCCGTG TCCCGGGGCC CCAAGTGCTT CAACAACGGC
    ACCTGCGTGG ACCAGGTGGG CGGCTACAGC TGCAGCTGCC CGCCCGGCTT CGTGGGTGAG
    CGCTGCGAGG GGGATGTCAA CGAGTGCCTG TCCAACCCCT GCGACGCCCG TGGCACCCAG
    AACTGCGTGC AGCACGTCAA CGCCTTCCAC TGCGAGTGCC GCGCCGGCCA CACCGGACGC
    CGCTGCGAGT CAGTCATCAA CGGCTGCAAA GACAGGCCCT GTAAGAATGG GGGTAGCTGT
    GCAGTGGCCT CCAACACGGC CCGTGGGTTC ATCTGCAAGT GCCCAGCGGG CTTCGAGGGC
    GCCACGTGCG AGAACGACGC CCGCAGCTGC GGGAGCCTGC GGTGCCTGAA CGGCGGCACG
    TGCATCGCGG GCCCGCGCAG CCCCACCTGC CTGTGCCTGG GCCCCTTCAC CGGCCCCGAG
    TGCCAGTTCC CGGCCAGCAG CCCCTGCGTG GGCGGCAACC CCTGCTACAA CCAGGGCGTC
    TGTGAGCCCA CCGCCGAGAG CCCCTTCTAC CGCTGCCGCT GCCCCGCCAA GTTCAACGGG
    CTCCTGTGCC ACATCCTGGA CTACAGCTTC GGGGGCGGCG TGGGCCTCGA CATCCCCCCG
    CCACAGATCG AGGAGACCTG CGAGCTCCCT GGCTGCCGCG AGGAGGCCGG CAACAAGGTC
    TGCAGCCTGC AGTGCAACAG CCACGCGTGC GGCTGGGACG GCGGCGACTG CTCCCTCGAC
    TTCGACGACC CTTGGCAGAA CTGCACGCAG TCCCTGCAGT GCTGGAAGTA CTTCAGCAAC
    GGCCGCTGCG ACAGCCAGTG CAACTCGGCC GGCTGCCTCT TCGACGGCTT CGACTGTCAG
    CGCGCAGAAG GCCAGTGCAA CCCCCTGTAC GACCAGTACT GCAAGGACCA CTTCAGGGAC
    GGGCACTGCG ACCAGGGCTG CAACAGTGCG GAGTGCGAGT GGGACGGGCT GGACTGTGCG
    GAGCATGTGC CCGAGCGGCT GGCGGCCGGC ACGCTGGTGC TGGTGGTGCT CATGCCGCCC
    GAGCAGCTGC GCAACCGCTC CCTGCACTTC CTGCGCGAGC TCAGCCGCCT GCTGCACACC
    AATGTGGTCT TCAAGCGTGA TGCCAGTGGC CAGCAGATGA TCTTCCCCTA CTACGGCCGC
    GAGGAGGAGC TGCACAAGCA CCCCGTCAGG CGCTCCGTGG ACGGTGGGGC CCCACTGCTC
    CTGGGCGGCG CGGGCAGACG CCAGCGCAGG GAGCTGGACC CCATGGACAT CCGCGGGTCC
    ATCGTCTACC TGGAGATCGA CAACCGGCAG TGCGTCCAGT CATCCTCCCA GTGCTTCCAG
    AGCGCCACGG ACGTGGCCGC CTTCTTGGGC GCGCTGGCCT CGCTGGGCAG CCTTAACATC
    CCCTACAAGA TCGAGGCTGT GCAGAGTGAG ACGGTGGAGC CGCCCCCGCC GCCGCCGCTG
    CACTTCATGT ATGTGGCTGT GGTGGCCTTC GTGTTGCTGT TCTTCGTGGG CTGCGGGGTG
    CTGCTGTCGC GGAAGCGCCG GCGGCAGCAT GGCCAGCTCT GGTTCCCTGA GGGCTTCAAG
    GTGTCGGAGG CCAGCAAGAA GAAGCGGCGC GAGCCCCTCG GCGAGGACTC CGTGGGCCTC
    AAGCCCCTGA AGAACTCCTC GGACGGCGCC CTCATGGACG ACAACCAGAA CGAGTGGGGC
    GACGAGGGCC TGGAGGCCAA GAAGTTCCGG TTCGAGGAGC CCGTGGTCCT CCCTGACCTG
    GACGACCAGA CGGACCACCG GCAGTGGACC CAGCAGCACC TGGACGCCGC CGACCTGCGC
    GTGTCTGCCA TGGCCCCCAC GCCTCCCCAG GGCGAGGCGG ATGCCGACTG CATGGACGTG
    AACGTCCGCG GGCCGGACGG CTTCACGCCC CTCATGATCG CCTCCTGCAG CGGAGGGGGC
    CTAGAGACTG GCAACAGCGA GGAGGAGGAG GACGCGCCCG CGGTCATCTC GGACTTCATC
    TACCAGGGCG CCAGCCTGCA CAACCAGACG GACCGCACCG GCGAGACCGC CCTGCACCTG
    GCCGCCCGCT ACTCGCGCTC CGACGCGGCC AAGCGCCTGC TGGAGGCCAG CGCGGACGCC
    AACATACAGG ACAACATGGG GCGCACCCCG CTGCACGCGG CCGTGTCTGC CGACGCACAG
    GGCGTTTTCC AGATCCTGAT CCGGAACCGA GCCACCGACC TGGATGCCCG CATGCACGAC
    GGCACGACCC CGCTGATCCT CGCTGCCCGC CTGGCCGTGG AGGGCATGCT GGAGGACCTC
    ATCAACTCTC ACGCCGACGT CAACGCTGTG GATGACCTGG GCAAGTCCGC CCTGCACTGG
    GCAGCCGCGG TGAACAACGT GGAGGCTGCT GTCGTGCTGC TGAAGAACGG GGCCAACAAG
    GACATGCAGA ACAACAAGGA GGAGACGCCC TTGTTCCTGG CCGCCCGGGA GGGCAGCTAC
    GAGACCGCCA AGGTGCTGCT GGACCATTTT GCCAACCGGG ACATCACAGA CCACATGGAC
    CGCCTGCCTC GCGACATCGC GCAGGAGCGT ATGCACCACG ACATCGTGCG GCTGCTGGAC
    GAGTACAGCC TGGTGCGCAG CCCCCCGCTG CATGGCGCCA CCCTGGGCGG CACGCCCACC
    CTGTCGCCCC CGCTCTGCTC GCCCAACGGC TACCTGGGCA ACCTCAAGCC CCCCATGCAG
    GGCAAGAAGG CCCGCAAGCC CAGCACCAAG GGCCTGGCCT GCGGCGGCAA GGAGCCCAAG
    GACCTCAAGG CTCGGAGGAA GAAGTCCCAA GATGGCAAGG GCTGTCTGCT GGACAGCGGG
    AGCGTGATGT CGCCCGTGGA CTCCCTCGAG TCCCCCCACG GCTACCTGTC AGACGTGGCC
    TCCCCACCCC TCCTGCCCTC CCCTTTCCAG CCGTCTCCCT CCGTGCCCCT CAACCACCTG
    CCCGGGATGC CCGAGACCCA CCTGGGCGTC AGCCACCTGA GCGTGGCGGC CAAGCCCGAG
    ATGGCGGTGC TGAGCGGGGG CAGCCGGCTG GCCTTCGAGG CAGGGCCACC ACGCCTCTCC
    CACCTGCCCG TGGCCTCCAG CACCAGCACG ATCCTGGGTT CGGGCGGCAG TGGGGGCAGT
    GGGGCTGTGA ATTTCACCGT GGGTGGAGCT GCGGGCTTGA ATGGCCAGTG CGAGTGGCTG
    TCCCGGCTGC AGAACGGCCT GGTGCCCAAC CAGTACAACC CGCTGCGAGG GGGCGTGACG
    CCGGGCACCC TGAGCACGCA GGCTGCTGGC CTCCAGCATG GCACGGTGGG CCCGCTGCAC
    GCCCCCGCCC TGTCCCAGGT GATGACCTAC CAGGCCCTGC CCAGCACGCG GCTGGCCTCC
    CAGCCTCACC TGGTGCAGCC GCAGCAGAAC TTACAGATGC AGCCGCCGAG CATGCCGCCG
    CAGCCAAACC TGCAGCCGCA CCTCGGCGTA AGCTCGGCCG CCAGCGGCCA CCTGGGCCGG
    AGCTTCCTGG GAGGGGAGCT GAGCCAGGCG GACATGCAGC CGCTGGGGCC CGGCAACCTG
    GCGGCGCACA CCGTCCTGCC GCAGGACGGC CAGGTCCTGC CCACATCGCT GCCGTCCACG
    CTGGCCCCGC CCACGATGGC CCCGCCCATG ACCACCGCCC AGTTCCTGAC GCCCCCCTCC
    CAGCACAGCT ACTCGTCCTC CCCCGTGGAC AACACCCCCA GCCACCAGCT ACAGGTGCCC
    GAGCACCCCT TCCTCACCCC GTCCCCGGAG TCCCCCGACC AGTGGTCCAG CTCCTCGCCA
    CATTCCAACA TCTCGGATTG GTCCGAGGGC ATCTCCAGCC CGCCCACCAG CGTGCCGTCC
    CAGATCGCCC ACGTCCCGGA GGCATTCAAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa56852
    Clone ID Related Accession (Same CDS sequence) XM_010810569.1
    Accession Version XM_010810569.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7617bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurogenic locus notch homolog protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGCCGCCGC TCCTGGCGCC CCTGCTTTGC CTGGCGTTGC TGCCTGCGCT CGCCGCACGA 
    GGCCTGCGCT GCTCCCAGCC CGGTGAGACC TGCTTGAACG GTGGGAAGTG TGAAGTGTTC
    CCCAACGGCA CCGAGGCCTG CATCTGCGGC GGGGCCTTCG CGGGCCAGCA ATGCCAGGCC
    CCCAACCCCT GCCTCAGCGC CCCCTGCAAG AACGGCGGGA CGTGCCACAC GACGGAGCGA
    GAAGGCCTGG TGGACTATGT CTGCGGCTGC CGCCTGGGCT TCTCGGGGCC ACTGTGCCTG
    ACGCCCCGCG ACCACGCTTG CCTCGCCAGC CCCTGCCTCA ACGGTGGCAC CTGTGATCTG
    CTCACGCTCA CTGAGTACAA GTGCCTGTGC ACCCCGGGCT GGTCAGGGAA GACGTGCCAG
    CAGGCCGACC CCTGCGCCTC CAACCCCTGT GCCAACGGTG GCCAGTGCCT GCCCTTTGAG
    GCGTCGTATA TCTGCCACTG CCCGCCCGGC TTCCACGGCC CCACGTGCAG GCAGGACGTC
    AACGAGTGCA GCCAGAGCCC CGGGCTCTGC CACCACGGCG GCACCTGCCT CAACGAGGTC
    GGCTCCTACC GCTGCGTCTG CCGCCCCACC CACACCGGCC CCCACTGCGA GCTGCCCTAC
    GTGCCCTGCA GTCCCTCCCC CTGCCAGAAC GGGGGCACCT GCCGCCCCAC GGGGGACACC
    ACCCACGAGT GCGCCTGCCT GCCAGGCTTC ACTGGCCAGA ACTGTGAGGA AAACATTGAC
    GACTGTCCGG GGAACAGCTG CAAGAACGGA GGCGCCTGTG TGGACGGCGT GAACACCTAC
    AACTGCCGCT GCCCCCCGGA GTGGACAGGT CAGTACTGCA CAGAGGACGT GGACGAGTGC
    CAGCTCATGC CCAACGCCTG CCAGAACGGC GGGACCTGCC ACAACACCCA CGGCGGCTAC
    AACTGCGTGT GCGTCAACGG CTGGACGGGC GAGGACTGCA GCGAGAACAT CGACGACTGC
    GCCAGCGCCT CCTGCTTCCA GGGCGCCACC TGCCACGACC GCGTCGCCTC CTTCTACTGC
    GAGTGTCCCC ACGGCCGCAC GGGTCTGCTG TGCCACCTGA ACGATGCCTG CATCAGCAAC
    CCCTGCAACG AGGGCTCCAA CTGCGACACC AACCCCGTCA ACGGCAAGGC CATCTGCACC
    TGCCCCTCGG GGTACACGGG GCCGGCCTGC AGCCAGGATG TGGACGAGTG CTCGCTGGGC
    GCCAACCCCT GTGAGCACGC GGGCAAGTGC ATCAACACGC TGGGCTCGTT CGAGTGCCAG
    TGTCTGCAGG GCTACACCGG CCCGCGCTGT GAGATCGACG TCAACGAGTG TGTATCGAAC
    CCGTGTCAGA ACGACGCCAC TTGTCTGGAT CAGATCGGGG AGTTCCAGTG TATCTGCATG
    CCTGGCTACG AGGGCTTGCA CTGCGAGGTG AACACGGACG AGTGTGCCAG CAGCCCCTGC
    CTGCAGAACG GCCGCTGCCT GGACAAGATC AACGAGTTCG TGTGCGAGTG CCCCACTGGC
    TTCACCGGGC ACTTGTGCCA GTATGACGTG GACGAGTGTG CAAGCACACC CTGCAAGAAC
    GGCGCTAAGT GCCTGGACGG GCCCAACACC TACACCTGCG TGTGCACAGA AGGCTACACG
    GGGCCCCACT GCGAGGTGGA CATCGACGAG TGTGACCCCG ACCCCTGCCA CTACGGGTCC
    TGCAAGGACG GCGTGGCCAC CTTCACCTGC CTGTGCCAGC CGGGCTACAC GGGCCACCAC
    TGTGAGAGCA ACATTAACGA GTGCCACAGC CAGCCCTGCC GCCACGGGGG CACCTGCCAG
    GACCGCGACA ACGCCTATCT CTGCTTCTGC CTCAAGGGGA CCACAGGACC CAACTGCGAG
    ATCAACCTGG ATGACTGCGC AAGCAACCCT TGCGACTCCG GCACGTGTCT GGACAAGATT
    GACGGCTACG AGTGTGCCTG TGAGCCAGGC TACACAGGGA GTATGTGTAA CATCAACATT
    GACGAGTGTG CGGACAGCCC TTGCCACAAC GGGGGCACCT GCGAGGATGG CATCAACGGC
    TTCACCTGTC GCTGCCCCGA GGGCTACCAC GACCCCACCT GCCTGTCTGA GGTCAACGAG
    TGCAGCAGCA ACCCCTGCAT CCATGGGGCC TGCCGCGACA GCCTCAACGG GTACAAGTGT
    GACTGTGACC CCGGGTGGAG TGGGGCCAAC TGTGACGTCA ACAACGACGA GTGTGAGTCG
    AACCCCTGCA TCAACGGGGG CACCTGCAAG GACATGACCA GCGGCTACGT GTGCGCCTGC
    CGCGAGGGCT TCAGTGGGCC CAACTGCCAG ACCAACATCA ACGAGTGCGC ATCCAACCCA
    TGTCTGAACC AGGGCACGTG TATCGATGAC GTGGCGGGGT ACAAATGCAA CTGCCTCTTG
    CCCTACACAG GGGCCACGTG TGAGGTGGTG CTGGCCCCAT GTGCCCCCGG GCCCTGCAGA
    AACGGCGGTG AGTGTCGGGA GTCAGAGGAC TATGAGAGCT TCTCCTGTGC CTGCCCCGCG
    GGCTGGCAGG GGCAGACCTG CGAGATCGAC ATCAACGAGT GCGTGAAGAG CCCGTGCCGC
    GCGGGCGCCT CCTGCCAGAA CACCAACGGC AGCTACCGCT GCCACTGCCA GGCGGGCTAC
    ACGGGGCGCA ACTGCGAGAC GGACATCGAC GACTGCCGGC CCAACCCGTG CCACAATGGG
    GGCTCCTGCA CAGATGGCAT CAACACGGCC TTCTGCGACT GCCTGCCCGG CTTCCAGGGC
    GCCTTCTGCG AGGAGGACAT CAACGAGTGC GCCAGCAGCC CCTGCCGCAA CGGCGCCAAC
    TGCACCGACT GTGTGGACAG CTACACCTGC ACCTGCCCCA CGGGCTTCAG CGGCATCCAC
    TGCGAGAACA ACACGCCCGA CTGCACGGAG AGCTCCTGCT TCAACGGCGG TACCTGCGTG
    GATGGCATCA ACTCCTTCAC CTGCCTGTGT CCACCTGGCT TCACGGGCAG CTACTGCCAA
    CATGACGTCA ACGAGTGTGA CTCTCGGCCC TGCCTGCACG GCGGCACCTG CCACGACAGC
    TACGGCACCT ACACGTGCAC CTGCCCGCAG GGCTACACAG GCCTCAACTG CCAGACCCTC
    GTGCGCTGGT GCGACTCGTC CCCCTGCAAG AATGACGGCC GGTGCTGGCA GACCAACGCG
    CTCTACCGCT GTGAGTGCCA CAGCGGCTGG ACCGGCCTCT ACTGTGACGT GCCCAGCGTC
    TCCTGCGAGG TGGCCGCGCG GCAGCAAGGC GTCAACGTGA CCCACTTGTG CCGGAACGGG
    GGCCTCTGCA TGAATGCGGG CAACACACAC CGCTGCCACT GCCAGGCCGG CTACACGGGC
    AGCTACTGCG AGGAGCAGGT GGACGAGTGC TCGCCCAGCC CCTGCCAGAA CGGGGCCACC
    TGCACCGACT ACCCCGGCGG CTACTCCTGT GAGTGTGTGG CCGGCTACCA CGGGGTGAAC
    TGCTCCGAGG AGGTGAACGA GTGCCTGTCC CAGCCCTGCC GGAACGGAGG CACCTGCATC
    GACCTCACCA ACACCTACAA GTGTTCCTGC CCCCGCGGCA CACAGGGCGT GCACTGCGAG
    ATCAACGTGG ACGACTGCAA CCCCCCCATC GACCCCGTGT CCCGGGGCCC CAAGTGCTTC
    AACAACGGCA CCTGCGTGGA CCAGGTGGGC GGCTACAGCT GCAGCTGCCC GCCCGGCTTC
    GTGGGTGAGC GCTGCGAGGG GGATGTCAAC GAGTGCCTGT CCAACCCCTG CGACGCCCGT
    GGCACCCAGA ACTGCGTGCA GCACGTCAAC GCCTTCCACT GCGAGTGCCG CGCCGGCCAC
    ACCGGACGCC GCTGCGAGTC AGTCATCAAC GGCTGCAAAG ACAGGCCCTG TAAGAATGGG
    GGTAGCTGTG CAGTGGCCTC CAACACGGCC CGTGGGTTCA TCTGCAAGTG CCCAGCGGGC
    TTCGAGGGCG CCACGTGCGA GAACGACGCC CGCAGCTGCG GGAGCCTGCG GTGCCTGAAC
    GGCGGCACGT GCATCGCGGG CCCGCGCAGC CCCACCTGCC TGTGCCTGGG CCCCTTCACC
    GGCCCCGAGT GCCAGTTCCC GGCCAGCAGC CCCTGCGTGG GCGGCAACCC CTGCTACAAC
    CAGGGCGTCT GTGAGCCCAC CGCCGAGAGC CCCTTCTACC GCTGCCGCTG CCCCGCCAAG
    TTCAACGGGC TCCTGTGCCA CATCCTGGAC TACAGCTTCG GGGGCGGCGT GGGCCTCGAC
    ATCCCCCCGC CACAGATCGA GGAGACCTGC GAGCTCCCTG GCTGCCGCGA GGAGGCCGGC
    AACAAGGTCT GCAGCCTGCA GTGCAACAGC CACGCGTGCG GCTGGGACGG CGGCGACTGC
    TCCCTCGACT TCGACGACCC TTGGCAGAAC TGCACGCAGT CCCTGCAGTG CTGGAAGTAC
    TTCAGCAACG GCCGCTGCGA CAGCCAGTGC AACTCGGCCG GCTGCCTCTT CGACGGCTTC
    GACTGTCAGC GCGCAGAAGG CCAGTGCAAC CCCCTGTACG ACCAGTACTG CAAGGACCAC
    TTCAGGGACG GGCACTGCGA CCAGGGCTGC AACAGTGCGG AGTGCGAGTG GGACGGGCTG
    GACTGTGCGG AGCATGTGCC CGAGCGGCTG GCGGCCGGCA CGCTGGTGCT GGTGGTGCTC
    ATGCCGCCCG AGCAGCTGCG CAACCGCTCC CTGCACTTCC TGCGCGAGCT CAGCCGCCTG
    CTGCACACCA ATGTGGTCTT CAAGCGTGAT GCCAGTGGCC AGCAGATGAT CTTCCCCTAC
    TACGGCCGAG CCCCACTGCC GGCAGGGGAG CGCTCCGAGG AGTGTCGGTG TGAGCACCAC
    GCATGCCCTG CTGGTGCTGG ACAAGGCGAG CCCTCAGGGC CACTGTGCAC CTCCAGACCT
    GGGCTCTCCC CCTACCTCAG GTCCATCGTC TACCTGGAGA TCGACAACCG GCAGTGCGTC
    CAGTCATCCT CCCAGTGCTT CCAGAGCGCC ACGGACGTGG CCGCCTTCTT GGGCGCGCTG
    GCCTCGCTGG GCAGCCTTAA CATCCCCTAC AAGATCGAGG CTGTGCAGAG TGAGACGGTG
    GAGCCGCCCC CGCCGCCGCC GCTGCACTTC ATGTATGTGG CTGTGGTGGC CTTCGTGTTG
    CTGTTCTTCG TGGGCTGCGG GGTGCTGCTG TCGCGGAAGC GCCGGCGGCA GCATGGCCAG
    CTCTGGTTCC CTGAGGGCTT CAAGGTGTCG GAGGCCAGCA AGAAGAAGCG GCGCGAGCCC
    CTCGGCGAGG ACTCCGTGGG CCTCAAGCCC CTGAAGAACT CCTCGGACGG CGCCCTCATG
    GACGACAACC AGAACGAGTG GGGCGACGAG GGCCTGGAGG CCAAGAAGTT CCGGTTCGAG
    GAGCCCGTGG TCCTCCCTGA CCTGGACGAC CAGACGGACC ACCGGCAGTG GACCCAGCAG
    CACCTGGACG CCGCCGACCT GCGCGTGTCT GCCATGGCCC CCACGCCTCC CCAGGGCGAG
    GCGGATGCCG ACTGCATGGA CGTGAACGTC CGCGGGCCGG ACGGCTTCAC GCCCCTCATG
    ATCGCCTCCT GCAGCGGAGG GGGCCTAGAG ACTGGCAACA GCGAGGAGGA GGAGGACGCG
    CCCGCGGTCA TCTCGGACTT CATCTACCAG GGCGCCAGCC TGCACAACCA GACGGACCGC
    ACCGGCGAGA CCGCCCTGCA CCTGGCCGCC CGCTACTCGC GCTCCGACGC GGCCAAGCGC
    CTGCTGGAGG CCAGCGCGGA CGCCAACATA CAGGACAACA TGGGGCGCAC CCCGCTGCAC
    GCGGCCGTGT CTGCCGACGC ACAGGGCGTT TTCCAGATCC TGATCCGGAA CCGAGCCACC
    GACCTGGATG CCCGCATGCA CGACGGCACG ACCCCGCTGA TCCTCGCTGC CCGCCTGGCC
    GTGGAGGGCA TGCTGGAGGA CCTCATCAAC TCTCACGCCG ACGTCAACGC TGTGGATGAC
    CTGGGCAAGT CCGCCCTGCA CTGGGCAGCC GCGGTGAACA ACGTGGAGGC TGCTGTCGTG
    CTGCTGAAGA ACGGGGCCAA CAAGGACATG CAGAACAACA AGGAGGAGAC GCCCTTGTTC
    CTGGCCGCCC GGGAGGGCAG CTACGAGACC GCCAAGGTGC TGCTGGACCA TTTTGCCAAC
    CGGGACATCA CAGACCACAT GGACCGCCTG CCTCGCGACA TCGCGCAGGA GCGTATGCAC
    CACGACATCG TGCGGCTGCT GGACGAGTAC AGCCTGGTGC GCAGCCCCCC GCTGCATGGC
    GCCACCCTGG GCGGCACGCC CACCCTGTCG CCCCCGCTCT GCTCGCCCAA CGGCTACCTG
    GGCAACCTCA AGCCCCCCAT GCAGGGCAAG AAGGCCCGCA AGCCCAGCAC CAAGGGCCTG
    GCCTGCGGCG GCAAGGAGCC CAAGGACCTC AAGGCTCGGA GGAAGAAGTC CCAAGATGGC
    AAGGGCTGTC TGCTGGACAG CGGGAGCGTG ATGTCGCCCG TGGACTCCCT CGAGTCCCCC
    CACGGCTACC TGTCAGACGT GGCCTCCCCA CCCCTCCTGC CCTCCCCTTT CCAGCCGTCT
    CCCTCCGTGC CCCTCAACCA CCTGCCCGGG ATGCCCGAGA CCCACCTGGG CGTCAGCCAC
    CTGAGCGTGG CGGCCAAGCC CGAGATGGCG GTGCTGAGCG GGGGCAGCCG GCTGGCCTTC
    GAGGCAGGGC CACCACGCCT CTCCCACCTG CCCGTGGCCT CCAGCACCAG CACGATCCTG
    GGTTCGGGCG GCAGTGGGGG CAGTGGGGCT GTGAATTTCA CCGTGGGTGG AGCTGCGGGC
    TTGAATGGCC AGTGCGAGTG GCTGTCCCGG CTGCAGAACG GCCTGGTGCC CAACCAGTAC
    AACCCGCTGC GAGGGGGCGT GACGCCGGGC ACCCTGAGCA CGCAGGCTGC TGGCCTCCAG
    CATGGCACGG TGGGCCCGCT GCACGCCCCC GCCCTGTCCC AGGTGATGAC CTACCAGGCC
    CTGCCCAGCA CGCGGCTGGC CTCCCAGCCT CACCTGGTGC AGCCGCAGCA GAACTTACAG
    ATGCAGCCGC CGAGCATGCC GCCGCAGCCA AACCTGCAGC CGCACCTCGG CGTAAGCTCG
    GCCGCCAGCG GCCACCTGGG CCGGAGCTTC CTGGGAGGGG AGCTGAGCCA GGCGGACATG
    CAGCCGCTGG GGCCCGGCAA CCTGGCGGCG CACACCGTCC TGCCGCAGGA CGGCCAGGTC
    CTGCCCACAT CGCTGCCGTC CACGCTGGCC CCGCCCACGA TGGCCCCGCC CATGACCACC
    GCCCAGTTCC TGACGCCCCC CTCCCAGCAC AGCTACTCGT CCTCCCCCGT GGACAACACC
    CCCAGCCACC AGCTACAGGT GCCCGAGCAC CCCTTCCTCA CCCCGTCCCC GGAGTCCCCC
    GACCAGTGGT CCAGCTCCTC GCCACATTCC AACATCTCGG ATTGGTCCGA GGGCATCTCC
    AGCCCGCCCA CCAGCGTGCC GTCCCAGATC GCCCACGTCC CGGAGGCATT CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808871.1
    CDS255..7871
    Translation

    Target ORF information:

    RefSeq Version XM_010810569.1
    Organism Bos taurus(cattle)
    Definition Bos taurus notch 1 (NOTCH1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810569.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGCCGCCGC TCCTGGCGCC CCTGCTTTGC CTGGCGTTGC TGCCTGCGCT CGCCGCACGA 
    GGCCTGCGCT GCTCCCAGCC CGGTGAGACC TGCTTGAACG GTGGGAAGTG TGAAGTGTTC
    CCCAACGGCA CCGAGGCCTG CATCTGCGGC GGGGCCTTCG CGGGCCAGCA ATGCCAGGCC
    CCCAACCCCT GCCTCAGCGC CCCCTGCAAG AACGGCGGGA CGTGCCACAC GACGGAGCGA
    GAAGGCCTGG TGGACTATGT CTGCGGCTGC CGCCTGGGCT TCTCGGGGCC ACTGTGCCTG
    ACGCCCCGCG ACCACGCTTG CCTCGCCAGC CCCTGCCTCA ACGGTGGCAC CTGTGATCTG
    CTCACGCTCA CTGAGTACAA GTGCCTGTGC ACCCCGGGCT GGTCAGGGAA GACGTGCCAG
    CAGGCCGACC CCTGCGCCTC CAACCCCTGT GCCAACGGTG GCCAGTGCCT GCCCTTTGAG
    GCGTCGTATA TCTGCCACTG CCCGCCCGGC TTCCACGGCC CCACGTGCAG GCAGGACGTC
    AACGAGTGCA GCCAGAGCCC CGGGCTCTGC CACCACGGCG GCACCTGCCT CAACGAGGTC
    GGCTCCTACC GCTGCGTCTG CCGCCCCACC CACACCGGCC CCCACTGCGA GCTGCCCTAC
    GTGCCCTGCA GTCCCTCCCC CTGCCAGAAC GGGGGCACCT GCCGCCCCAC GGGGGACACC
    ACCCACGAGT GCGCCTGCCT GCCAGGCTTC ACTGGCCAGA ACTGTGAGGA AAACATTGAC
    GACTGTCCGG GGAACAGCTG CAAGAACGGA GGCGCCTGTG TGGACGGCGT GAACACCTAC
    AACTGCCGCT GCCCCCCGGA GTGGACAGGT CAGTACTGCA CAGAGGACGT GGACGAGTGC
    CAGCTCATGC CCAACGCCTG CCAGAACGGC GGGACCTGCC ACAACACCCA CGGCGGCTAC
    AACTGCGTGT GCGTCAACGG CTGGACGGGC GAGGACTGCA GCGAGAACAT CGACGACTGC
    GCCAGCGCCT CCTGCTTCCA GGGCGCCACC TGCCACGACC GCGTCGCCTC CTTCTACTGC
    GAGTGTCCCC ACGGCCGCAC GGGTCTGCTG TGCCACCTGA ACGATGCCTG CATCAGCAAC
    CCCTGCAACG AGGGCTCCAA CTGCGACACC AACCCCGTCA ACGGCAAGGC CATCTGCACC
    TGCCCCTCGG GGTACACGGG GCCGGCCTGC AGCCAGGATG TGGACGAGTG CTCGCTGGGC
    GCCAACCCCT GTGAGCACGC GGGCAAGTGC ATCAACACGC TGGGCTCGTT CGAGTGCCAG
    TGTCTGCAGG GCTACACCGG CCCGCGCTGT GAGATCGACG TCAACGAGTG TGTATCGAAC
    CCGTGTCAGA ACGACGCCAC TTGTCTGGAT CAGATCGGGG AGTTCCAGTG TATCTGCATG
    CCTGGCTACG AGGGCTTGCA CTGCGAGGTG AACACGGACG AGTGTGCCAG CAGCCCCTGC
    CTGCAGAACG GCCGCTGCCT GGACAAGATC AACGAGTTCG TGTGCGAGTG CCCCACTGGC
    TTCACCGGGC ACTTGTGCCA GTATGACGTG GACGAGTGTG CAAGCACACC CTGCAAGAAC
    GGCGCTAAGT GCCTGGACGG GCCCAACACC TACACCTGCG TGTGCACAGA AGGCTACACG
    GGGCCCCACT GCGAGGTGGA CATCGACGAG TGTGACCCCG ACCCCTGCCA CTACGGGTCC
    TGCAAGGACG GCGTGGCCAC CTTCACCTGC CTGTGCCAGC CGGGCTACAC GGGCCACCAC
    TGTGAGAGCA ACATTAACGA GTGCCACAGC CAGCCCTGCC GCCACGGGGG CACCTGCCAG
    GACCGCGACA ACGCCTATCT CTGCTTCTGC CTCAAGGGGA CCACAGGACC CAACTGCGAG
    ATCAACCTGG ATGACTGCGC AAGCAACCCT TGCGACTCCG GCACGTGTCT GGACAAGATT
    GACGGCTACG AGTGTGCCTG TGAGCCAGGC TACACAGGGA GTATGTGTAA CATCAACATT
    GACGAGTGTG CGGACAGCCC TTGCCACAAC GGGGGCACCT GCGAGGATGG CATCAACGGC
    TTCACCTGTC GCTGCCCCGA GGGCTACCAC GACCCCACCT GCCTGTCTGA GGTCAACGAG
    TGCAGCAGCA ACCCCTGCAT CCATGGGGCC TGCCGCGACA GCCTCAACGG GTACAAGTGT
    GACTGTGACC CCGGGTGGAG TGGGGCCAAC TGTGACGTCA ACAACGACGA GTGTGAGTCG
    AACCCCTGCA TCAACGGGGG CACCTGCAAG GACATGACCA GCGGCTACGT GTGCGCCTGC
    CGCGAGGGCT TCAGTGGGCC CAACTGCCAG ACCAACATCA ACGAGTGCGC ATCCAACCCA
    TGTCTGAACC AGGGCACGTG TATCGATGAC GTGGCGGGGT ACAAATGCAA CTGCCTCTTG
    CCCTACACAG GGGCCACGTG TGAGGTGGTG CTGGCCCCAT GTGCCCCCGG GCCCTGCAGA
    AACGGCGGTG AGTGTCGGGA GTCAGAGGAC TATGAGAGCT TCTCCTGTGC CTGCCCCGCG
    GGCTGGCAGG GGCAGACCTG CGAGATCGAC ATCAACGAGT GCGTGAAGAG CCCGTGCCGC
    GCGGGCGCCT CCTGCCAGAA CACCAACGGC AGCTACCGCT GCCACTGCCA GGCGGGCTAC
    ACGGGGCGCA ACTGCGAGAC GGACATCGAC GACTGCCGGC CCAACCCGTG CCACAATGGG
    GGCTCCTGCA CAGATGGCAT CAACACGGCC TTCTGCGACT GCCTGCCCGG CTTCCAGGGC
    GCCTTCTGCG AGGAGGACAT CAACGAGTGC GCCAGCAGCC CCTGCCGCAA CGGCGCCAAC
    TGCACCGACT GTGTGGACAG CTACACCTGC ACCTGCCCCA CGGGCTTCAG CGGCATCCAC
    TGCGAGAACA ACACGCCCGA CTGCACGGAG AGCTCCTGCT TCAACGGCGG TACCTGCGTG
    GATGGCATCA ACTCCTTCAC CTGCCTGTGT CCACCTGGCT TCACGGGCAG CTACTGCCAA
    CATGACGTCA ACGAGTGTGA CTCTCGGCCC TGCCTGCACG GCGGCACCTG CCACGACAGC
    TACGGCACCT ACACGTGCAC CTGCCCGCAG GGCTACACAG GCCTCAACTG CCAGACCCTC
    GTGCGCTGGT GCGACTCGTC CCCCTGCAAG AATGACGGCC GGTGCTGGCA GACCAACGCG
    CTCTACCGCT GTGAGTGCCA CAGCGGCTGG ACCGGCCTCT ACTGTGACGT GCCCAGCGTC
    TCCTGCGAGG TGGCCGCGCG GCAGCAAGGC GTCAACGTGA CCCACTTGTG CCGGAACGGG
    GGCCTCTGCA TGAATGCGGG CAACACACAC CGCTGCCACT GCCAGGCCGG CTACACGGGC
    AGCTACTGCG AGGAGCAGGT GGACGAGTGC TCGCCCAGCC CCTGCCAGAA CGGGGCCACC
    TGCACCGACT ACCCCGGCGG CTACTCCTGT GAGTGTGTGG CCGGCTACCA CGGGGTGAAC
    TGCTCCGAGG AGGTGAACGA GTGCCTGTCC CAGCCCTGCC GGAACGGAGG CACCTGCATC
    GACCTCACCA ACACCTACAA GTGTTCCTGC CCCCGCGGCA CACAGGGCGT GCACTGCGAG
    ATCAACGTGG ACGACTGCAA CCCCCCCATC GACCCCGTGT CCCGGGGCCC CAAGTGCTTC
    AACAACGGCA CCTGCGTGGA CCAGGTGGGC GGCTACAGCT GCAGCTGCCC GCCCGGCTTC
    GTGGGTGAGC GCTGCGAGGG GGATGTCAAC GAGTGCCTGT CCAACCCCTG CGACGCCCGT
    GGCACCCAGA ACTGCGTGCA GCACGTCAAC GCCTTCCACT GCGAGTGCCG CGCCGGCCAC
    ACCGGACGCC GCTGCGAGTC AGTCATCAAC GGCTGCAAAG ACAGGCCCTG TAAGAATGGG
    GGTAGCTGTG CAGTGGCCTC CAACACGGCC CGTGGGTTCA TCTGCAAGTG CCCAGCGGGC
    TTCGAGGGCG CCACGTGCGA GAACGACGCC CGCAGCTGCG GGAGCCTGCG GTGCCTGAAC
    GGCGGCACGT GCATCGCGGG CCCGCGCAGC CCCACCTGCC TGTGCCTGGG CCCCTTCACC
    GGCCCCGAGT GCCAGTTCCC GGCCAGCAGC CCCTGCGTGG GCGGCAACCC CTGCTACAAC
    CAGGGCGTCT GTGAGCCCAC CGCCGAGAGC CCCTTCTACC GCTGCCGCTG CCCCGCCAAG
    TTCAACGGGC TCCTGTGCCA CATCCTGGAC TACAGCTTCG GGGGCGGCGT GGGCCTCGAC
    ATCCCCCCGC CACAGATCGA GGAGACCTGC GAGCTCCCTG GCTGCCGCGA GGAGGCCGGC
    AACAAGGTCT GCAGCCTGCA GTGCAACAGC CACGCGTGCG GCTGGGACGG CGGCGACTGC
    TCCCTCGACT TCGACGACCC TTGGCAGAAC TGCACGCAGT CCCTGCAGTG CTGGAAGTAC
    TTCAGCAACG GCCGCTGCGA CAGCCAGTGC AACTCGGCCG GCTGCCTCTT CGACGGCTTC
    GACTGTCAGC GCGCAGAAGG CCAGTGCAAC CCCCTGTACG ACCAGTACTG CAAGGACCAC
    TTCAGGGACG GGCACTGCGA CCAGGGCTGC AACAGTGCGG AGTGCGAGTG GGACGGGCTG
    GACTGTGCGG AGCATGTGCC CGAGCGGCTG GCGGCCGGCA CGCTGGTGCT GGTGGTGCTC
    ATGCCGCCCG AGCAGCTGCG CAACCGCTCC CTGCACTTCC TGCGCGAGCT CAGCCGCCTG
    CTGCACACCA ATGTGGTCTT CAAGCGTGAT GCCAGTGGCC AGCAGATGAT CTTCCCCTAC
    TACGGCCGAG CCCCACTGCC GGCAGGGGAG CGCTCCGAGG AGTGTCGGTG TGAGCACCAC
    GCATGCCCTG CTGGTGCTGG ACAAGGCGAG CCCTCAGGGC CACTGTGCAC CTCCAGACCT
    GGGCTCTCCC CCTACCTCAG GTCCATCGTC TACCTGGAGA TCGACAACCG GCAGTGCGTC
    CAGTCATCCT CCCAGTGCTT CCAGAGCGCC ACGGACGTGG CCGCCTTCTT GGGCGCGCTG
    GCCTCGCTGG GCAGCCTTAA CATCCCCTAC AAGATCGAGG CTGTGCAGAG TGAGACGGTG
    GAGCCGCCCC CGCCGCCGCC GCTGCACTTC ATGTATGTGG CTGTGGTGGC CTTCGTGTTG
    CTGTTCTTCG TGGGCTGCGG GGTGCTGCTG TCGCGGAAGC GCCGGCGGCA GCATGGCCAG
    CTCTGGTTCC CTGAGGGCTT CAAGGTGTCG GAGGCCAGCA AGAAGAAGCG GCGCGAGCCC
    CTCGGCGAGG ACTCCGTGGG CCTCAAGCCC CTGAAGAACT CCTCGGACGG CGCCCTCATG
    GACGACAACC AGAACGAGTG GGGCGACGAG GGCCTGGAGG CCAAGAAGTT CCGGTTCGAG
    GAGCCCGTGG TCCTCCCTGA CCTGGACGAC CAGACGGACC ACCGGCAGTG GACCCAGCAG
    CACCTGGACG CCGCCGACCT GCGCGTGTCT GCCATGGCCC CCACGCCTCC CCAGGGCGAG
    GCGGATGCCG ACTGCATGGA CGTGAACGTC CGCGGGCCGG ACGGCTTCAC GCCCCTCATG
    ATCGCCTCCT GCAGCGGAGG GGGCCTAGAG ACTGGCAACA GCGAGGAGGA GGAGGACGCG
    CCCGCGGTCA TCTCGGACTT CATCTACCAG GGCGCCAGCC TGCACAACCA GACGGACCGC
    ACCGGCGAGA CCGCCCTGCA CCTGGCCGCC CGCTACTCGC GCTCCGACGC GGCCAAGCGC
    CTGCTGGAGG CCAGCGCGGA CGCCAACATA CAGGACAACA TGGGGCGCAC CCCGCTGCAC
    GCGGCCGTGT CTGCCGACGC ACAGGGCGTT TTCCAGATCC TGATCCGGAA CCGAGCCACC
    GACCTGGATG CCCGCATGCA CGACGGCACG ACCCCGCTGA TCCTCGCTGC CCGCCTGGCC
    GTGGAGGGCA TGCTGGAGGA CCTCATCAAC TCTCACGCCG ACGTCAACGC TGTGGATGAC
    CTGGGCAAGT CCGCCCTGCA CTGGGCAGCC GCGGTGAACA ACGTGGAGGC TGCTGTCGTG
    CTGCTGAAGA ACGGGGCCAA CAAGGACATG CAGAACAACA AGGAGGAGAC GCCCTTGTTC
    CTGGCCGCCC GGGAGGGCAG CTACGAGACC GCCAAGGTGC TGCTGGACCA TTTTGCCAAC
    CGGGACATCA CAGACCACAT GGACCGCCTG CCTCGCGACA TCGCGCAGGA GCGTATGCAC
    CACGACATCG TGCGGCTGCT GGACGAGTAC AGCCTGGTGC GCAGCCCCCC GCTGCATGGC
    GCCACCCTGG GCGGCACGCC CACCCTGTCG CCCCCGCTCT GCTCGCCCAA CGGCTACCTG
    GGCAACCTCA AGCCCCCCAT GCAGGGCAAG AAGGCCCGCA AGCCCAGCAC CAAGGGCCTG
    GCCTGCGGCG GCAAGGAGCC CAAGGACCTC AAGGCTCGGA GGAAGAAGTC CCAAGATGGC
    AAGGGCTGTC TGCTGGACAG CGGGAGCGTG ATGTCGCCCG TGGACTCCCT CGAGTCCCCC
    CACGGCTACC TGTCAGACGT GGCCTCCCCA CCCCTCCTGC CCTCCCCTTT CCAGCCGTCT
    CCCTCCGTGC CCCTCAACCA CCTGCCCGGG ATGCCCGAGA CCCACCTGGG CGTCAGCCAC
    CTGAGCGTGG CGGCCAAGCC CGAGATGGCG GTGCTGAGCG GGGGCAGCCG GCTGGCCTTC
    GAGGCAGGGC CACCACGCCT CTCCCACCTG CCCGTGGCCT CCAGCACCAG CACGATCCTG
    GGTTCGGGCG GCAGTGGGGG CAGTGGGGCT GTGAATTTCA CCGTGGGTGG AGCTGCGGGC
    TTGAATGGCC AGTGCGAGTG GCTGTCCCGG CTGCAGAACG GCCTGGTGCC CAACCAGTAC
    AACCCGCTGC GAGGGGGCGT GACGCCGGGC ACCCTGAGCA CGCAGGCTGC TGGCCTCCAG
    CATGGCACGG TGGGCCCGCT GCACGCCCCC GCCCTGTCCC AGGTGATGAC CTACCAGGCC
    CTGCCCAGCA CGCGGCTGGC CTCCCAGCCT CACCTGGTGC AGCCGCAGCA GAACTTACAG
    ATGCAGCCGC CGAGCATGCC GCCGCAGCCA AACCTGCAGC CGCACCTCGG CGTAAGCTCG
    GCCGCCAGCG GCCACCTGGG CCGGAGCTTC CTGGGAGGGG AGCTGAGCCA GGCGGACATG
    CAGCCGCTGG GGCCCGGCAA CCTGGCGGCG CACACCGTCC TGCCGCAGGA CGGCCAGGTC
    CTGCCCACAT CGCTGCCGTC CACGCTGGCC CCGCCCACGA TGGCCCCGCC CATGACCACC
    GCCCAGTTCC TGACGCCCCC CTCCCAGCAC AGCTACTCGT CCTCCCCCGT GGACAACACC
    CCCAGCCACC AGCTACAGGT GCCCGAGCAC CCCTTCCTCA CCCCGTCCCC GGAGTCCCCC
    GACCAGTGGT CCAGCTCCTC GCCACATTCC AACATCTCGG ATTGGTCCGA GGGCATCTCC
    AGCCCGCCCA CCAGCGTGCC GTCCCAGATC GCCCACGTCC CGGAGGCATT CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94696
    Clone ID Related Accession (Same CDS sequence) XM_015465548.1
    Accession Version XM_015465548.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurogenic locus notch homolog protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGCCGCCGC TCCTGGCGCC CCTGCTTTGC CTGGCGTTGC TGCCTGCGCT CGCCGCACGA 
    GGCCTGCGCT GCTCCCAGCC CGGTGAGACC TGCTTGAACG GTGGGAAGTG TGAAGTGTTC
    CCCAACGGCA CCGAGGCCTG CATCTGCGGC GGGGCCTTCG CGGGCCAGCA ATGCCAGGCC
    CCCAACCCCT GCCTCAGCGC CCCCTGCAAG AACGGCGGGA CGTGCCACAC GACGGAGCGA
    GAAGGCCTGG TGGACTATGT CTGCGGCTGC CGCCTGGGCT TCTCGGGGCC ACTGTGCCTG
    ACGCCCCGCG ACCACGCTTG CCTCGCCAGC CCCTGCCTCA ACGGTGGCAC CTGTGATCTG
    CTCACGCTCA CTGAGTACAA GTGCCTGTGC ACCCCGGGCT GGTCAGGGAA GACGTGCCAG
    CAGGCCGACC CCTGCGCCTC CAACCCCTGT GCCAACGGTG GCCAGTGCCT GCCCTTTGAG
    GCGTCGTATA TCTGCCACTG CCCGCCCGGC TTCCACGGCC CCACGTGCAG GCAGGACGTC
    AACGAGTGCA GCCAGAGCCC CGGGCTCTGC CACCACGGCG GCACCTGCCT CAACGAGGTC
    GGCTCCTACC GCTGCGTCTG CCGCCCCACC CACACCGGCC CCCACTGCGA GCTGCCCTAC
    GTGCCCTGCA GTCCCTCCCC CTGCCAGAAC GGGGGCACCT GCCGCCCCAC GGGGGACACC
    ACCCACGAGT GCGCCTGCCT GCCAGGCTTC ACTGGCCAGA ACTGTGAGGA AAACATTGAC
    GACTGTCCGG GGAACAGCTG CAAGAACGGA GGCGCCTGTG TGGACGGCGT GAACACCTAC
    AACTGCCGCT GCCCCCCGGA GTGGACAGGT CAGTACTGCA CAGAGGACGT GGACGAGTGC
    CAGCTCATGC CCAACGCCTG CCAGAACGGC GGGACCTGCC ACAACACCCA CGGCGGCTAC
    AACTGCGTGT GCGTCAACGG CTGGACGGGC GAGGACTGCA GCGAGAACAT CGACGACTGC
    GCCAGCGCCT CCTGCTTCCA GGGCGCCACC TGCCACGACC GCGTCGCCTC CTTCTACTGC
    GAGTGTCCCC ACGGCCGCAC GGGTCTGCTG TGCCACCTGA ACGATGCCTG CATCAGCAAC
    CCCTGCAACG AGGGCTCCAA CTGCGACACC AACCCCGTCA ACGGCAAGGC CATCTGCACC
    TGCCCCTCGG GGTACACGGG GCCGGCCTGC AGCCAGGATG TGGACGAGTG CTCGCTGGGC
    GCCAACCCCT GTGAGCACGC GGGCAAGTGC ATCAACACGC TGGGCTCGTT CGAGTGCCAG
    TGTCTGCAGG GCTACACCGG CCCGCGCTGT GAGATCGACG TCAACGAGTG TGTATCGAAC
    CCGTGTCAGA ACGACGCCAC TTGTCTGGAT CAGATCGGGG AGTTCCAGTG TATCTGCATG
    CCTGGCTACG AGGGCTTGCA CTGCGAGGTG AACACGGACG AGTGTGCCAG CAGCCCCTGC
    CTGCAGAACG GCCGCTGCCT GGACAAGATC AACGAGTTCG TGTGCGAGTG CCCCACTGGC
    TTCACCGGGC ACTTGTGCCA GTATGACGTG GACGAGTGTG CAAGCACACC CTGCAAGAAC
    GGCGCTAAGT GCCTGGACGG GCCCAACACC TACACCTGCG TGTGCACAGA AGGCTACACG
    GGGCCCCACT GCGAGGTGGA CATCGACGAG TGTGACCCCG ACCCCTGCCA CTACGGGTCC
    TGCAAGGACG GCGTGGCCAC CTTCACCTGC CTGTGCCAGC CGGGCTACAC GGGCCACCAC
    TGTGAGAGCA ACATTAACGA GTGCCACAGC CAGCCCTGCC GCCACGGGGG CACCTGCCAG
    GACCGCGACA ACGCCTATCT CTGCTTCTGC CTCAAGGGGA CCACAGGACC CAACTGCGAG
    ATCAACCTGG ATGACTGCGC AAGCAACCCT TGCGACTCCG GCACGTGTCT GGACAAGATT
    GACGGCTACG AGTGTGCCTG TGAGCCAGGC TACACAGGGA GTATGTGTAA CATCAACATT
    GACGAGTGTG CGGACAGCCC TTGCCACAAC GGGGGCACCT GCGAGGATGG CATCAACGGC
    TTCACCTGTC GCTGCCCCGA GGGCTACCAC GACCCCACCT GCCTGTCTGA GGTCAACGAG
    TGCAGCAGCA ACCCCTGCAT CCATGGGGCC TGCCGCGACA GCCTCAACGG GTACAAGTGT
    GACTGTGACC CCGGGTGGAG TGGGGCCAAC TGTGACGTCA ACAACGACGA GTGTGAGTCG
    AACCCCTGCA TCAACGGGGG CACCTGCAAG GACATGACCA GCGGCTACGT GTGCGCCTGC
    CGCGAGGGCT TCAGTGGGCC CAACTGCCAG ACCAACATCA ACGAGTGCGC ATCCAACCCA
    TGTCTGAACC AGGGCACGTG TATCGATGAC GTGGCGGGGT ACAAATGCAA CTGCCTCTTG
    CCCTACACAG GGGCCACGTG TGAGGTGGTG CTGGCCCCAT GTGCCCCCGG GCCCTGCAGA
    AACGGCGGTG AGTGTCGGGA GTCAGAGGAC TATGAGAGCT TCTCCTGTGC CTGCCCCGCG
    GGCTGGCAGG GGCAGACCTG CGAGATCGAC ATCAACGAGT GCGTGAAGAG CCCGTGCCGC
    GCGGGCGCCT CCTGCCAGAA CACCAACGGC AGCTACCGCT GCCACTGCCA GGCGGGCTAC
    ACGGGGCGCA ACTGCGAGAC GGACATCGAC GACTGCCGGC CCAACCCGTG CCACAATGGG
    GGCTCCTGCA CAGATGGCAT CAACACGGCC TTCTGCGACT GCCTGCCCGG CTTCCAGGGC
    GCCTTCTGCG AGGAGGACAT CAACGAGTGC GCCAGCAGCC CCTGCCGCAA CGGCGCCAAC
    TGCACCGACT GTGTGGACAG CTACACCTGC ACCTGCCCCA CGGGCTTCAG CGGCATCCAC
    TGCGAGAACA ACACGCCCGA CTGCACGGAG AGCTCCTGCT TCAACGGCGG TACCTGCGTG
    GATGGCATCA ACTCCTTCAC CTGCCTGTGT CCACCTGGCT TCACGGGCAG CTACTGCCAA
    CATGACGTCA ACGAGTGTGA CTCTCGGCCC TGCCTGCACG GCGGCACCTG CCACGACAGC
    TACGGCACCT ACACGTGCAC CTGCCCGCAG GGCTACACAG GCCTCAACTG CCAGACCCTC
    GTGCGCTGGT GCGACTCGTC CCCCTGCAAG AATGACGGCC GGTGCTGGCA GACCAACGCG
    CTCTACCGCT GTGAGTGCCA CAGCGGCTGG ACCGGCCTCT ACTGTGACGT GCCCAGCGTC
    TCCTGCGAGG TGGCCGCGCG GCAGCAAGGC GTCAACGTGA CCCACTTGTG CCGGAACGGG
    GGCCTCTGCA TGAATGCGGG CAACACACAC CGCTGCCACT GCCAGGCCGG CTACACGGGC
    AGCTACTGCG AGGAGCAGGT GGACGAGTGC TCGCCCAGCC CCTGCCAGAA CGGGGCCACC
    TGCACCGACT ACCCCGGCGG CTACTCCTGT GAGTGTGTGG CCGGCTACCA CGGGGTGAAC
    TGCTCCGAGG AGGTGAACGA GTGCCTGTCC CAGCCCTGCC GGAACGGAGG CACCTGCATC
    GACCTCACCA ACACCTACAA GTGTTCCTGC CCCCGCGGCA CACAGGGCGT GCACTGCGAG
    ATCAACGTGG ACGACTGCAA CCCCCCCATC GACCCCGTGT CCCGGGGCCC CAAGTGCTTC
    AACAACGGCA CCTGCGTGGA CCAGGTGGGC GGCTACAGCT GCAGCTGCCC GCCCGGCTTC
    GTGGGTGAGC GCTGCGAGGG GGATGTCAAC GAGTGCCTGT CCAACCCCTG CGACGCCCGT
    GGCACCCAGA ACTGCGTGCA GCACGTCAAC GCCTTCCACT GCGAGTGCCG CGCCGGCCAC
    ACCGGACGCC GCTGCGAGTC AGTCATCAAC GGCTGCAAAG ACAGGCCCTG TAAGAATGGG
    GGTAGCTGTG CAGTGGCCTC CAACACGGCC CGTGGGTTCA TCTGCAAGTG CCCAGCGGGC
    TTCGAGGGCG CCACGTGCGA GAACGACGCC CGCAGCTGCG GGAGCCTGCG GTGCCTGAAC
    GGCGGCACGT GCATCGCGGG CCCGCGCAGC CCCACCTGCC TGTGCCTGGG CCCCTTCACC
    GGCCCCGAGT GCCAGTTCCC GGCCAGCAGC CCCTGCGTGG GCGGCAACCC CTGCTACAAC
    CAGGGCGTCT GTGAGCCCAC CGCCGAGAGC CCCTTCTACC GCTGCCGCTG CCCCGCCAAG
    TTCAACGGGC TCCTGTGCCA CATCCTGGAC TACAGCTTCG GGGGCGGCGT GGGCCTCGAC
    ATCCCCCCGC CACAGATCGA GGAGACCTGC GAGCTCCCTG GCTGCCGCGA GGAGGCCGGC
    AACAAGGTCT GCAGCCTGCA GTGCAACAGC CACGCGTGCG GCTGGGACGG CGGCGACTGC
    TCCCTCGACT TCGACGACCC TTGGCAGAAC TGCACGCAGT CCCTGCAGTG CTGGAAGTAC
    TTCAGCAACG GCCGCTGCGA CAGCCAGTGC AACTCGGCCG GCTGCCTCTT CGACGGCTTC
    GACTGTCAGC GCGCAGAAGG CCAGTGCAAC CCCCTGTACG ACCAGTACTG CAAGGACCAC
    TTCAGGGACG GGCACTGCGA CCAGGGCTGC AACAGTGCGG AGTGCGAGTG GGACGGGCTG
    GACTGTGCGG AGCATGTGCC CGAGCGGCTG GCGGCCGGCA CGCTGGTGCT GGTGGTGCTC
    ATGCCGCCCG AGCAGCTGCG CAACCGCTCC CTGCACTTCC TGCGCGAGCT CAGCCGCCTG
    CTGCACACCA ATGTGGTCTT CAAGCGTGAT GCCAGTGGCC AGCAGATGAT CTTCCCCTAC
    TACGGCCGCG GGAGGAGCTG CACAAGCACC CCGTCAGGCT CCGTGGACGG TGGGGCCCCA
    CTGCTCCTGG GCGGCGCGGG CAGACGCCAG CGCAGGGAGC TGGACCCCAT GGACATCCGC
    GGGTCCATCG TCTACCTGGA GATCGACAAC CGGCAGTGCG TCCAGTCATC CTCCCAGTGC
    TTCCAGAGCG CCACGGACGT GGCCGCCTTC TTGGGCGCGC TGGCCTCGCT GGGCAGCCTT
    AACATCCCCT ACAAGATCGA GGCTGTGCAG AGTGAGACGG TGGAGCCGCC CCCGCCGCCG
    CCGCTGCACT TCATGTATGT GGCTGTGGTG GCCTTCGTGT TGCTGTTCTT CGTGGGCTGC
    GGGGTGCTGC TGTCGCGGAA GCGCCGGCGG CAGCATGGCC AGCTCTGGTT CCCTGAGGGC
    TTCAAGGTGT CGGAGGCCAG CAAGAAGAAG CGGCGCGAGC CCCTCGGCGA GGACTCCGTG
    GGCCTCAAGC CCCTGAAGAA CTCCTCGGAC GGCGCCCTCA TGGACGACAA CCAGAACGAG
    TGGGGCGACG AGGGCCTGGA GGCCAAGAAG TTCCGGTTCG AGGAGCCCGT GGTCCTCCCT
    GACCTGGACG ACCAGACGGA CCACCGGCAG TGGACCCAGC AGCACCTGGA CGCCGCCGAC
    CTGCGCGTGT CTGCCATGGC CCCCACGCCT CCCCAGGGCG AGGCGGATGC CGACTGCATG
    GACGTGAACG TCCGCGGGCC GGACGGCTTC ACGCCCCTCA TGATCGCCTC CTGCAGCGGA
    GGGGGCCTAG AGACTGGCAA CAGCGAGGAG GAGGAGGACG CGCCCGCGGT CATCTCGGAC
    TTCATCTACC AGGGCGCCAG CCTGCACAAC CAGACGGACC GCACCGGCGA GACCGCCCTG
    CACCTGGCCG CCCGCTACTC GCGCTCCGAC GCGGCCAAGC GCCTGCTGGA GGCCAGCGCG
    GACGCCAACA TACAGGACAA CATGGGGCGC ACCCCGCTGC ACGCGGCCGT GTCTGCCGAC
    GCACAGGGCG TTTTCCAGAT CCTGATCCGG AACCGAGCCA CCGACCTGGA TGCCCGCATG
    CACGACGGCA CGACCCCGCT GATCCTCGCT GCCCGCCTGG CCGTGGAGGG CATGCTGGAG
    GACCTCATCA ACTCTCACGC CGACGTCAAC GCTGTGGATG ACCTGGGCAA GTCCGCCCTG
    CACTGGGCAG CCGCGGTGAA CAACGTGGAG GCTGCTGTCG TGCTGCTGAA GAACGGGGCC
    AACAAGGACA TGCAGAACAA CAAGGAGGAG ACGCCCTTGT TCCTGGCCGC CCGGGAGGGC
    AGCTACGAGA CCGCCAAGGT GCTGCTGGAC CATTTTGCCA ACCGGGACAT CACAGACCAC
    ATGGACCGCC TGCCTCGCGA CATCGCGCAG GAGCGTATGC ACCACGACAT CGTGCGGCTG
    CTGGACGAGT ACAGCCTGGT GCGCAGCCCC CCGCTGCATG GCGCCACCCT GGGCGGCACG
    CCCACCCTGT CGCCCCCGCT CTGCTCGCCC AACGGCTACC TGGGCAACCT CAAGCCCCCC
    ATGCAGGGCA AGAAGGCCCG CAAGCCCAGC ACCAAGGGCC TGGCCTGCGG CGGCAAGGAG
    CCCAAGGACC TCAAGGCTCG GAGGAAGAAG TCCCAAGATG GCAAGGGCTG TCTGCTGGAC
    AGCGGGAGCG TGATGTCGCC CGTGGACTCC CTCGAGTCCC CCCACGGCTA CCTGTCAGAC
    GTGGCCTCCC CACCCCTCCT GCCCTCCCCT TTCCAGCCGT CTCCCTCCGT GCCCCTCAAC
    CACCTGCCCG GGATGCCCGA GACCCACCTG GGCGTCAGCC ACCTGAGCGT GGCGGCCAAG
    CCCGAGATGG CGGTGCTGAG CGGGGGCAGC CGGCTGGCCT TCGAGGCAGG GCCACCACGC
    CTCTCCCACC TGCCCGTGGC CTCCAGCACC AGCACGATCC TGGGTTCGGG CGGCAGTGGG
    GGCAGTGGGG CTGTGAATTT CACCGTGGGT GGAGCTGCGG GCTTGAATGG CCAGTGCGAG
    TGGCTGTCCC GGCTGCAGAA CGGCCTGGTG CCCAACCAGT ACAACCCGCT GCGAGGGGGC
    GTGACGCCGG GCACCCTGAG CACGCAGGCT GCTGGCCTCC AGCATGGCAC GGTGGGCCCG
    CTGCACGCCC CCGCCCTGTC CCAGGTGATG ACCTACCAGG CCCTGCCCAG CACGCGGCTG
    GCCTCCCAGC CTCACCTGGT GCAGCCGCAG CAGAACTTAC AGATGCAGCC GCCGAGCATG
    CCGCCGCAGC CAAACCTGCA GCCGCACCTC GGCGTAAGCT CGGCCGCCAG CGGCCACCTG
    GGCCGGAGCT TCCTGGGAGG GGAGCTGAGC CAGGCGGACA TGCAGCCGCT GGGGCCCGGC
    AACCTGGCGG CGCACACCGT CCTGCCGCAG GACGGCCAGG TCCTGCCCAC ATCGCTGCCG
    TCCACGCTGG CCCCGCCCAC GATGGCCCCG CCCATGACCA CCGCCCAGTT CCTGACGCCC
    CCCTCCCAGC ACAGCTACTC GTCCTCCCCC GTGGACAACA CCCCCAGCCA CCAGCTACAG
    GTGCCCGAGC ACCCCTTCCT CACCCCGTCC CCGGAGTCCC CCGACCAGTG GTCCAGCTCC
    TCGCCACATT CCAACATCTC GGATTGGTCC GAGGGCATCT CCAGCCCGCC CACCAGCGTG
    CCGTCCCAGA TCGCCCACGT CCCGGAGGCA TTCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321034.1
    CDS255..7853
    Translation

    Target ORF information:

    RefSeq Version XM_015465548.1
    Organism Bos taurus(cattle)
    Definition Bos taurus notch 1 (NOTCH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465548.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGCCGCCGC TCCTGGCGCC CCTGCTTTGC CTGGCGTTGC TGCCTGCGCT CGCCGCACGA 
    GGCCTGCGCT GCTCCCAGCC CGGTGAGACC TGCTTGAACG GTGGGAAGTG TGAAGTGTTC
    CCCAACGGCA CCGAGGCCTG CATCTGCGGC GGGGCCTTCG CGGGCCAGCA ATGCCAGGCC
    CCCAACCCCT GCCTCAGCGC CCCCTGCAAG AACGGCGGGA CGTGCCACAC GACGGAGCGA
    GAAGGCCTGG TGGACTATGT CTGCGGCTGC CGCCTGGGCT TCTCGGGGCC ACTGTGCCTG
    ACGCCCCGCG ACCACGCTTG CCTCGCCAGC CCCTGCCTCA ACGGTGGCAC CTGTGATCTG
    CTCACGCTCA CTGAGTACAA GTGCCTGTGC ACCCCGGGCT GGTCAGGGAA GACGTGCCAG
    CAGGCCGACC CCTGCGCCTC CAACCCCTGT GCCAACGGTG GCCAGTGCCT GCCCTTTGAG
    GCGTCGTATA TCTGCCACTG CCCGCCCGGC TTCCACGGCC CCACGTGCAG GCAGGACGTC
    AACGAGTGCA GCCAGAGCCC CGGGCTCTGC CACCACGGCG GCACCTGCCT CAACGAGGTC
    GGCTCCTACC GCTGCGTCTG CCGCCCCACC CACACCGGCC CCCACTGCGA GCTGCCCTAC
    GTGCCCTGCA GTCCCTCCCC CTGCCAGAAC GGGGGCACCT GCCGCCCCAC GGGGGACACC
    ACCCACGAGT GCGCCTGCCT GCCAGGCTTC ACTGGCCAGA ACTGTGAGGA AAACATTGAC
    GACTGTCCGG GGAACAGCTG CAAGAACGGA GGCGCCTGTG TGGACGGCGT GAACACCTAC
    AACTGCCGCT GCCCCCCGGA GTGGACAGGT CAGTACTGCA CAGAGGACGT GGACGAGTGC
    CAGCTCATGC CCAACGCCTG CCAGAACGGC GGGACCTGCC ACAACACCCA CGGCGGCTAC
    AACTGCGTGT GCGTCAACGG CTGGACGGGC GAGGACTGCA GCGAGAACAT CGACGACTGC
    GCCAGCGCCT CCTGCTTCCA GGGCGCCACC TGCCACGACC GCGTCGCCTC CTTCTACTGC
    GAGTGTCCCC ACGGCCGCAC GGGTCTGCTG TGCCACCTGA ACGATGCCTG CATCAGCAAC
    CCCTGCAACG AGGGCTCCAA CTGCGACACC AACCCCGTCA ACGGCAAGGC CATCTGCACC
    TGCCCCTCGG GGTACACGGG GCCGGCCTGC AGCCAGGATG TGGACGAGTG CTCGCTGGGC
    GCCAACCCCT GTGAGCACGC GGGCAAGTGC ATCAACACGC TGGGCTCGTT CGAGTGCCAG
    TGTCTGCAGG GCTACACCGG CCCGCGCTGT GAGATCGACG TCAACGAGTG TGTATCGAAC
    CCGTGTCAGA ACGACGCCAC TTGTCTGGAT CAGATCGGGG AGTTCCAGTG TATCTGCATG
    CCTGGCTACG AGGGCTTGCA CTGCGAGGTG AACACGGACG AGTGTGCCAG CAGCCCCTGC
    CTGCAGAACG GCCGCTGCCT GGACAAGATC AACGAGTTCG TGTGCGAGTG CCCCACTGGC
    TTCACCGGGC ACTTGTGCCA GTATGACGTG GACGAGTGTG CAAGCACACC CTGCAAGAAC
    GGCGCTAAGT GCCTGGACGG GCCCAACACC TACACCTGCG TGTGCACAGA AGGCTACACG
    GGGCCCCACT GCGAGGTGGA CATCGACGAG TGTGACCCCG ACCCCTGCCA CTACGGGTCC
    TGCAAGGACG GCGTGGCCAC CTTCACCTGC CTGTGCCAGC CGGGCTACAC GGGCCACCAC
    TGTGAGAGCA ACATTAACGA GTGCCACAGC CAGCCCTGCC GCCACGGGGG CACCTGCCAG
    GACCGCGACA ACGCCTATCT CTGCTTCTGC CTCAAGGGGA CCACAGGACC CAACTGCGAG
    ATCAACCTGG ATGACTGCGC AAGCAACCCT TGCGACTCCG GCACGTGTCT GGACAAGATT
    GACGGCTACG AGTGTGCCTG TGAGCCAGGC TACACAGGGA GTATGTGTAA CATCAACATT
    GACGAGTGTG CGGACAGCCC TTGCCACAAC GGGGGCACCT GCGAGGATGG CATCAACGGC
    TTCACCTGTC GCTGCCCCGA GGGCTACCAC GACCCCACCT GCCTGTCTGA GGTCAACGAG
    TGCAGCAGCA ACCCCTGCAT CCATGGGGCC TGCCGCGACA GCCTCAACGG GTACAAGTGT
    GACTGTGACC CCGGGTGGAG TGGGGCCAAC TGTGACGTCA ACAACGACGA GTGTGAGTCG
    AACCCCTGCA TCAACGGGGG CACCTGCAAG GACATGACCA GCGGCTACGT GTGCGCCTGC
    CGCGAGGGCT TCAGTGGGCC CAACTGCCAG ACCAACATCA ACGAGTGCGC ATCCAACCCA
    TGTCTGAACC AGGGCACGTG TATCGATGAC GTGGCGGGGT ACAAATGCAA CTGCCTCTTG
    CCCTACACAG GGGCCACGTG TGAGGTGGTG CTGGCCCCAT GTGCCCCCGG GCCCTGCAGA
    AACGGCGGTG AGTGTCGGGA GTCAGAGGAC TATGAGAGCT TCTCCTGTGC CTGCCCCGCG
    GGCTGGCAGG GGCAGACCTG CGAGATCGAC ATCAACGAGT GCGTGAAGAG CCCGTGCCGC
    GCGGGCGCCT CCTGCCAGAA CACCAACGGC AGCTACCGCT GCCACTGCCA GGCGGGCTAC
    ACGGGGCGCA ACTGCGAGAC GGACATCGAC GACTGCCGGC CCAACCCGTG CCACAATGGG
    GGCTCCTGCA CAGATGGCAT CAACACGGCC TTCTGCGACT GCCTGCCCGG CTTCCAGGGC
    GCCTTCTGCG AGGAGGACAT CAACGAGTGC GCCAGCAGCC CCTGCCGCAA CGGCGCCAAC
    TGCACCGACT GTGTGGACAG CTACACCTGC ACCTGCCCCA CGGGCTTCAG CGGCATCCAC
    TGCGAGAACA ACACGCCCGA CTGCACGGAG AGCTCCTGCT TCAACGGCGG TACCTGCGTG
    GATGGCATCA ACTCCTTCAC CTGCCTGTGT CCACCTGGCT TCACGGGCAG CTACTGCCAA
    CATGACGTCA ACGAGTGTGA CTCTCGGCCC TGCCTGCACG GCGGCACCTG CCACGACAGC
    TACGGCACCT ACACGTGCAC CTGCCCGCAG GGCTACACAG GCCTCAACTG CCAGACCCTC
    GTGCGCTGGT GCGACTCGTC CCCCTGCAAG AATGACGGCC GGTGCTGGCA GACCAACGCG
    CTCTACCGCT GTGAGTGCCA CAGCGGCTGG ACCGGCCTCT ACTGTGACGT GCCCAGCGTC
    TCCTGCGAGG TGGCCGCGCG GCAGCAAGGC GTCAACGTGA CCCACTTGTG CCGGAACGGG
    GGCCTCTGCA TGAATGCGGG CAACACACAC CGCTGCCACT GCCAGGCCGG CTACACGGGC
    AGCTACTGCG AGGAGCAGGT GGACGAGTGC TCGCCCAGCC CCTGCCAGAA CGGGGCCACC
    TGCACCGACT ACCCCGGCGG CTACTCCTGT GAGTGTGTGG CCGGCTACCA CGGGGTGAAC
    TGCTCCGAGG AGGTGAACGA GTGCCTGTCC CAGCCCTGCC GGAACGGAGG CACCTGCATC
    GACCTCACCA ACACCTACAA GTGTTCCTGC CCCCGCGGCA CACAGGGCGT GCACTGCGAG
    ATCAACGTGG ACGACTGCAA CCCCCCCATC GACCCCGTGT CCCGGGGCCC CAAGTGCTTC
    AACAACGGCA CCTGCGTGGA CCAGGTGGGC GGCTACAGCT GCAGCTGCCC GCCCGGCTTC
    GTGGGTGAGC GCTGCGAGGG GGATGTCAAC GAGTGCCTGT CCAACCCCTG CGACGCCCGT
    GGCACCCAGA ACTGCGTGCA GCACGTCAAC GCCTTCCACT GCGAGTGCCG CGCCGGCCAC
    ACCGGACGCC GCTGCGAGTC AGTCATCAAC GGCTGCAAAG ACAGGCCCTG TAAGAATGGG
    GGTAGCTGTG CAGTGGCCTC CAACACGGCC CGTGGGTTCA TCTGCAAGTG CCCAGCGGGC
    TTCGAGGGCG CCACGTGCGA GAACGACGCC CGCAGCTGCG GGAGCCTGCG GTGCCTGAAC
    GGCGGCACGT GCATCGCGGG CCCGCGCAGC CCCACCTGCC TGTGCCTGGG CCCCTTCACC
    GGCCCCGAGT GCCAGTTCCC GGCCAGCAGC CCCTGCGTGG GCGGCAACCC CTGCTACAAC
    CAGGGCGTCT GTGAGCCCAC CGCCGAGAGC CCCTTCTACC GCTGCCGCTG CCCCGCCAAG
    TTCAACGGGC TCCTGTGCCA CATCCTGGAC TACAGCTTCG GGGGCGGCGT GGGCCTCGAC
    ATCCCCCCGC CACAGATCGA GGAGACCTGC GAGCTCCCTG GCTGCCGCGA GGAGGCCGGC
    AACAAGGTCT GCAGCCTGCA GTGCAACAGC CACGCGTGCG GCTGGGACGG CGGCGACTGC
    TCCCTCGACT TCGACGACCC TTGGCAGAAC TGCACGCAGT CCCTGCAGTG CTGGAAGTAC
    TTCAGCAACG GCCGCTGCGA CAGCCAGTGC AACTCGGCCG GCTGCCTCTT CGACGGCTTC
    GACTGTCAGC GCGCAGAAGG CCAGTGCAAC CCCCTGTACG ACCAGTACTG CAAGGACCAC
    TTCAGGGACG GGCACTGCGA CCAGGGCTGC AACAGTGCGG AGTGCGAGTG GGACGGGCTG
    GACTGTGCGG AGCATGTGCC CGAGCGGCTG GCGGCCGGCA CGCTGGTGCT GGTGGTGCTC
    ATGCCGCCCG AGCAGCTGCG CAACCGCTCC CTGCACTTCC TGCGCGAGCT CAGCCGCCTG
    CTGCACACCA ATGTGGTCTT CAAGCGTGAT GCCAGTGGCC AGCAGATGAT CTTCCCCTAC
    TACGGCCGCG GGAGGAGCTG CACAAGCACC CCGTCAGGCT CCGTGGACGG TGGGGCCCCA
    CTGCTCCTGG GCGGCGCGGG CAGACGCCAG CGCAGGGAGC TGGACCCCAT GGACATCCGC
    GGGTCCATCG TCTACCTGGA GATCGACAAC CGGCAGTGCG TCCAGTCATC CTCCCAGTGC
    TTCCAGAGCG CCACGGACGT GGCCGCCTTC TTGGGCGCGC TGGCCTCGCT GGGCAGCCTT
    AACATCCCCT ACAAGATCGA GGCTGTGCAG AGTGAGACGG TGGAGCCGCC CCCGCCGCCG
    CCGCTGCACT TCATGTATGT GGCTGTGGTG GCCTTCGTGT TGCTGTTCTT CGTGGGCTGC
    GGGGTGCTGC TGTCGCGGAA GCGCCGGCGG CAGCATGGCC AGCTCTGGTT CCCTGAGGGC
    TTCAAGGTGT CGGAGGCCAG CAAGAAGAAG CGGCGCGAGC CCCTCGGCGA GGACTCCGTG
    GGCCTCAAGC CCCTGAAGAA CTCCTCGGAC GGCGCCCTCA TGGACGACAA CCAGAACGAG
    TGGGGCGACG AGGGCCTGGA GGCCAAGAAG TTCCGGTTCG AGGAGCCCGT GGTCCTCCCT
    GACCTGGACG ACCAGACGGA CCACCGGCAG TGGACCCAGC AGCACCTGGA CGCCGCCGAC
    CTGCGCGTGT CTGCCATGGC CCCCACGCCT CCCCAGGGCG AGGCGGATGC CGACTGCATG
    GACGTGAACG TCCGCGGGCC GGACGGCTTC ACGCCCCTCA TGATCGCCTC CTGCAGCGGA
    GGGGGCCTAG AGACTGGCAA CAGCGAGGAG GAGGAGGACG CGCCCGCGGT CATCTCGGAC
    TTCATCTACC AGGGCGCCAG CCTGCACAAC CAGACGGACC GCACCGGCGA GACCGCCCTG
    CACCTGGCCG CCCGCTACTC GCGCTCCGAC GCGGCCAAGC GCCTGCTGGA GGCCAGCGCG
    GACGCCAACA TACAGGACAA CATGGGGCGC ACCCCGCTGC ACGCGGCCGT GTCTGCCGAC
    GCACAGGGCG TTTTCCAGAT CCTGATCCGG AACCGAGCCA CCGACCTGGA TGCCCGCATG
    CACGACGGCA CGACCCCGCT GATCCTCGCT GCCCGCCTGG CCGTGGAGGG CATGCTGGAG
    GACCTCATCA ACTCTCACGC CGACGTCAAC GCTGTGGATG ACCTGGGCAA GTCCGCCCTG
    CACTGGGCAG CCGCGGTGAA CAACGTGGAG GCTGCTGTCG TGCTGCTGAA GAACGGGGCC
    AACAAGGACA TGCAGAACAA CAAGGAGGAG ACGCCCTTGT TCCTGGCCGC CCGGGAGGGC
    AGCTACGAGA CCGCCAAGGT GCTGCTGGAC CATTTTGCCA ACCGGGACAT CACAGACCAC
    ATGGACCGCC TGCCTCGCGA CATCGCGCAG GAGCGTATGC ACCACGACAT CGTGCGGCTG
    CTGGACGAGT ACAGCCTGGT GCGCAGCCCC CCGCTGCATG GCGCCACCCT GGGCGGCACG
    CCCACCCTGT CGCCCCCGCT CTGCTCGCCC AACGGCTACC TGGGCAACCT CAAGCCCCCC
    ATGCAGGGCA AGAAGGCCCG CAAGCCCAGC ACCAAGGGCC TGGCCTGCGG CGGCAAGGAG
    CCCAAGGACC TCAAGGCTCG GAGGAAGAAG TCCCAAGATG GCAAGGGCTG TCTGCTGGAC
    AGCGGGAGCG TGATGTCGCC CGTGGACTCC CTCGAGTCCC CCCACGGCTA CCTGTCAGAC
    GTGGCCTCCC CACCCCTCCT GCCCTCCCCT TTCCAGCCGT CTCCCTCCGT GCCCCTCAAC
    CACCTGCCCG GGATGCCCGA GACCCACCTG GGCGTCAGCC ACCTGAGCGT GGCGGCCAAG
    CCCGAGATGG CGGTGCTGAG CGGGGGCAGC CGGCTGGCCT TCGAGGCAGG GCCACCACGC
    CTCTCCCACC TGCCCGTGGC CTCCAGCACC AGCACGATCC TGGGTTCGGG CGGCAGTGGG
    GGCAGTGGGG CTGTGAATTT CACCGTGGGT GGAGCTGCGG GCTTGAATGG CCAGTGCGAG
    TGGCTGTCCC GGCTGCAGAA CGGCCTGGTG CCCAACCAGT ACAACCCGCT GCGAGGGGGC
    GTGACGCCGG GCACCCTGAG CACGCAGGCT GCTGGCCTCC AGCATGGCAC GGTGGGCCCG
    CTGCACGCCC CCGCCCTGTC CCAGGTGATG ACCTACCAGG CCCTGCCCAG CACGCGGCTG
    GCCTCCCAGC CTCACCTGGT GCAGCCGCAG CAGAACTTAC AGATGCAGCC GCCGAGCATG
    CCGCCGCAGC CAAACCTGCA GCCGCACCTC GGCGTAAGCT CGGCCGCCAG CGGCCACCTG
    GGCCGGAGCT TCCTGGGAGG GGAGCTGAGC CAGGCGGACA TGCAGCCGCT GGGGCCCGGC
    AACCTGGCGG CGCACACCGT CCTGCCGCAG GACGGCCAGG TCCTGCCCAC ATCGCTGCCG
    TCCACGCTGG CCCCGCCCAC GATGGCCCCG CCCATGACCA CCGCCCAGTT CCTGACGCCC
    CCCTCCCAGC ACAGCTACTC GTCCTCCCCC GTGGACAACA CCCCCAGCCA CCAGCTACAG
    GTGCCCGAGC ACCCCTTCCT CACCCCGTCC CCGGAGTCCC CCGACCAGTG GTCCAGCTCC
    TCGCCACATT CCAACATCTC GGATTGGTCC GAGGGCATCT CCAGCCCGCC CACCAGCGTG
    CCGTCCCAGA TCGCCCACGT CCCGGAGGCA TTCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94697
    Clone ID Related Accession (Same CDS sequence) XM_015465549.1
    Accession Version XM_015465549.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurogenic locus notch homolog protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGCAGGCCC TGCCCACAGG TCAGTACTGC ACAGAGGACG TGGACGAGTG CCAGCTCATG 
    CCCAACGCCT GCCAGAACGG CGGGACCTGC CACAACACCC ACGGCGGCTA CAACTGCGTG
    TGCGTCAACG GCTGGACGGG CGAGGACTGC AGCGAGAACA TCGACGACTG CGCCAGCGCC
    TCCTGCTTCC AGGGCGCCAC CTGCCACGAC CGCGTCGCCT CCTTCTACTG CGAGTGTCCC
    CACGGCCGCA CGGGTCTGCT GTGCCACCTG AACGATGCCT GCATCAGCAA CCCCTGCAAC
    GAGGGCTCCA ACTGCGACAC CAACCCCGTC AACGGCAAGG CCATCTGCAC CTGCCCCTCG
    GGGTACACGG GGCCGGCCTG CAGCCAGGAT GTGGACGAGT GCTCGCTGGG CGCCAACCCC
    TGTGAGCACG CGGGCAAGTG CATCAACACG CTGGGCTCGT TCGAGTGCCA GTGTCTGCAG
    GGCTACACCG GCCCGCGCTG TGAGATCGAC GTCAACGAGT GTGTATCGAA CCCGTGTCAG
    AACGACGCCA CTTGTCTGGA TCAGATCGGG GAGTTCCAGT GTATCTGCAT GCCTGGCTAC
    GAGGGCTTGC ACTGCGAGGT GAACACGGAC GAGTGTGCCA GCAGCCCCTG CCTGCAGAAC
    GGCCGCTGCC TGGACAAGAT CAACGAGTTC GTGTGCGAGT GCCCCACTGG CTTCACCGGG
    CACTTGTGCC AGTATGACGT GGACGAGTGT GCAAGCACAC CCTGCAAGAA CGGCGCTAAG
    TGCCTGGACG GGCCCAACAC CTACACCTGC GTGTGCACAG AAGGCTACAC GGGGCCCCAC
    TGCGAGGTGG ACATCGACGA GTGTGACCCC GACCCCTGCC ACTACGGGTC CTGCAAGGAC
    GGCGTGGCCA CCTTCACCTG CCTGTGCCAG CCGGGCTACA CGGGCCACCA CTGTGAGAGC
    AACATTAACG AGTGCCACAG CCAGCCCTGC CGCCACGGGG GCACCTGCCA GGACCGCGAC
    AACGCCTATC TCTGCTTCTG CCTCAAGGGG ACCACAGGAC CCAACTGCGA GATCAACCTG
    GATGACTGCG CAAGCAACCC TTGCGACTCC GGCACGTGTC TGGACAAGAT TGACGGCTAC
    GAGTGTGCCT GTGAGCCAGG CTACACAGGG AGTATGTGTA ACATCAACAT TGACGAGTGT
    GCGGACAGCC CTTGCCACAA CGGGGGCACC TGCGAGGATG GCATCAACGG CTTCACCTGT
    CGCTGCCCCG AGGGCTACCA CGACCCCACC TGCCTGTCTG AGGTCAACGA GTGCAGCAGC
    AACCCCTGCA TCCATGGGGC CTGCCGCGAC AGCCTCAACG GGTACAAGTG TGACTGTGAC
    CCCGGGTGGA GTGGGGCCAA CTGTGACGTC AACAACGACG AGTGTGAGTC GAACCCCTGC
    ATCAACGGGG GCACCTGCAA GGACATGACC AGCGGCTACG TGTGCGCCTG CCGCGAGGGC
    TTCAGTGGGC CCAACTGCCA GACCAACATC AACGAGTGCG CATCCAACCC ATGTCTGAAC
    CAGGGCACGT GTATCGATGA CGTGGCGGGG TACAAATGCA ACTGCCTCTT GCCCTACACA
    GGGGCCACGT GTGAGGTGGT GCTGGCCCCA TGTGCCCCCG GGCCCTGCAG AAACGGCGGT
    GAGTGTCGGG AGTCAGAGGA CTATGAGAGC TTCTCCTGTG CCTGCCCCGC GGGCTGGCAG
    GGGCAGACCT GCGAGATCGA CATCAACGAG TGCGTGAAGA GCCCGTGCCG CGCGGGCGCC
    TCCTGCCAGA ACACCAACGG CAGCTACCGC TGCCACTGCC AGGCGGGCTA CACGGGGCGC
    AACTGCGAGA CGGACATCGA CGACTGCCGG CCCAACCCGT GCCACAATGG GGGCTCCTGC
    ACAGATGGCA TCAACACGGC CTTCTGCGAC TGCCTGCCCG GCTTCCAGGG CGCCTTCTGC
    GAGGAGGACA TCAACGAGTG CGCCAGCAGC CCCTGCCGCA ACGGCGCCAA CTGCACCGAC
    TGTGTGGACA GCTACACCTG CACCTGCCCC ACGGGCTTCA GCGGCATCCA CTGCGAGAAC
    AACACGCCCG ACTGCACGGA GAGCTCCTGC TTCAACGGCG GTACCTGCGT GGATGGCATC
    AACTCCTTCA CCTGCCTGTG TCCACCTGGC TTCACGGGCA GCTACTGCCA ACATGACGTC
    AACGAGTGTG ACTCTCGGCC CTGCCTGCAC GGCGGCACCT GCCACGACAG CTACGGCACC
    TACACGTGCA CCTGCCCGCA GGGCTACACA GGCCTCAACT GCCAGACCCT CGTGCGCTGG
    TGCGACTCGT CCCCCTGCAA GAATGACGGC CGGTGCTGGC AGACCAACGC GCTCTACCGC
    TGTGAGTGCC ACAGCGGCTG GACCGGCCTC TACTGTGACG TGCCCAGCGT CTCCTGCGAG
    GTGGCCGCGC GGCAGCAAGG CGTCAACGTG ACCCACTTGT GCCGGAACGG GGGCCTCTGC
    ATGAATGCGG GCAACACACA CCGCTGCCAC TGCCAGGCCG GCTACACGGG CAGCTACTGC
    GAGGAGCAGG TGGACGAGTG CTCGCCCAGC CCCTGCCAGA ACGGGGCCAC CTGCACCGAC
    TACCCCGGCG GCTACTCCTG TGAGTGTGTG GCCGGCTACC ACGGGGTGAA CTGCTCCGAG
    GAGGTGAACG AGTGCCTGTC CCAGCCCTGC CGGAACGGAG GCACCTGCAT CGACCTCACC
    AACACCTACA AGTGTTCCTG CCCCCGCGGC ACACAGGGCG TGCACTGCGA GATCAACGTG
    GACGACTGCA ACCCCCCCAT CGACCCCGTG TCCCGGGGCC CCAAGTGCTT CAACAACGGC
    ACCTGCGTGG ACCAGGTGGG CGGCTACAGC TGCAGCTGCC CGCCCGGCTT CGTGGGTGAG
    CGCTGCGAGG GGGATGTCAA CGAGTGCCTG TCCAACCCCT GCGACGCCCG TGGCACCCAG
    AACTGCGTGC AGCACGTCAA CGCCTTCCAC TGCGAGTGCC GCGCCGGCCA CACCGGACGC
    CGCTGCGAGT CAGTCATCAA CGGCTGCAAA GACAGGCCCT GTAAGAATGG GGGTAGCTGT
    GCAGTGGCCT CCAACACGGC CCGTGGGTTC ATCTGCAAGT GCCCAGCGGG CTTCGAGGGC
    GCCACGTGCG AGAACGACGC CCGCAGCTGC GGGAGCCTGC GGTGCCTGAA CGGCGGCACG
    TGCATCGCGG GCCCGCGCAG CCCCACCTGC CTGTGCCTGG GCCCCTTCAC CGGCCCCGAG
    TGCCAGTTCC CGGCCAGCAG CCCCTGCGTG GGCGGCAACC CCTGCTACAA CCAGGGCGTC
    TGTGAGCCCA CCGCCGAGAG CCCCTTCTAC CGCTGCCGCT GCCCCGCCAA GTTCAACGGG
    CTCCTGTGCC ACATCCTGGA CTACAGCTTC GGGGGCGGCG TGGGCCTCGA CATCCCCCCG
    CCACAGATCG AGGAGACCTG CGAGCTCCCT GGCTGCCGCG AGGAGGCCGG CAACAAGGTC
    TGCAGCCTGC AGTGCAACAG CCACGCGTGC GGCTGGGACG GCGGCGACTG CTCCCTCGAC
    TTCGACGACC CTTGGCAGAA CTGCACGCAG TCCCTGCAGT GCTGGAAGTA CTTCAGCAAC
    GGCCGCTGCG ACAGCCAGTG CAACTCGGCC GGCTGCCTCT TCGACGGCTT CGACTGTCAG
    CGCGCAGAAG GCCAGTGCAA CCCCCTGTAC GACCAGTACT GCAAGGACCA CTTCAGGGAC
    GGGCACTGCG ACCAGGGCTG CAACAGTGCG GAGTGCGAGT GGGACGGGCT GGACTGTGCG
    GAGCATGTGC CCGAGCGGCT GGCGGCCGGC ACGCTGGTGC TGGTGGTGCT CATGCCGCCC
    GAGCAGCTGC GCAACCGCTC CCTGCACTTC CTGCGCGAGC TCAGCCGCCT GCTGCACACC
    AATGTGGTCT TCAAGCGTGA TGCCAGTGGC CAGCAGATGA TCTTCCCCTA CTACGGCCGC
    GGGAGGAGCT GCACAAGCAC CCCGTCAGGC TCCGTGGACG GTGGGGCCCC ACTGCTCCTG
    GGCGGCGCGG GCAGACGCCA GCGCAGGGAG CTGGACCCCA TGGACATCCG CGGGTCCATC
    GTCTACCTGG AGATCGACAA CCGGCAGTGC GTCCAGTCAT CCTCCCAGTG CTTCCAGAGC
    GCCACGGACG TGGCCGCCTT CTTGGGCGCG CTGGCCTCGC TGGGCAGCCT TAACATCCCC
    TACAAGATCG AGGCTGTGCA GAGTGAGACG GTGGAGCCGC CCCCGCCGCC GCCGCTGCAC
    TTCATGTATG TGGCTGTGGT GGCCTTCGTG TTGCTGTTCT TCGTGGGCTG CGGGGTGCTG
    CTGTCGCGGA AGCGCCGGCG GCAGCATGGC CAGCTCTGGT TCCCTGAGGG CTTCAAGGTG
    TCGGAGGCCA GCAAGAAGAA GCGGCGCGAG CCCCTCGGCG AGGACTCCGT GGGCCTCAAG
    CCCCTGAAGA ACTCCTCGGA CGGCGCCCTC ATGGACGACA ACCAGAACGA GTGGGGCGAC
    GAGGGCCTGG AGGCCAAGAA GTTCCGGTTC GAGGAGCCCG TGGTCCTCCC TGACCTGGAC
    GACCAGACGG ACCACCGGCA GTGGACCCAG CAGCACCTGG ACGCCGCCGA CCTGCGCGTG
    TCTGCCATGG CCCCCACGCC TCCCCAGGGC GAGGCGGATG CCGACTGCAT GGACGTGAAC
    GTCCGCGGGC CGGACGGCTT CACGCCCCTC ATGATCGCCT CCTGCAGCGG AGGGGGCCTA
    GAGACTGGCA ACAGCGAGGA GGAGGAGGAC GCGCCCGCGG TCATCTCGGA CTTCATCTAC
    CAGGGCGCCA GCCTGCACAA CCAGACGGAC CGCACCGGCG AGACCGCCCT GCACCTGGCC
    GCCCGCTACT CGCGCTCCGA CGCGGCCAAG CGCCTGCTGG AGGCCAGCGC GGACGCCAAC
    ATACAGGACA ACATGGGGCG CACCCCGCTG CACGCGGCCG TGTCTGCCGA CGCACAGGGC
    GTTTTCCAGA TCCTGATCCG GAACCGAGCC ACCGACCTGG ATGCCCGCAT GCACGACGGC
    ACGACCCCGC TGATCCTCGC TGCCCGCCTG GCCGTGGAGG GCATGCTGGA GGACCTCATC
    AACTCTCACG CCGACGTCAA CGCTGTGGAT GACCTGGGCA AGTCCGCCCT GCACTGGGCA
    GCCGCGGTGA ACAACGTGGA GGCTGCTGTC GTGCTGCTGA AGAACGGGGC CAACAAGGAC
    ATGCAGAACA ACAAGGAGGA GACGCCCTTG TTCCTGGCCG CCCGGGAGGG CAGCTACGAG
    ACCGCCAAGG TGCTGCTGGA CCATTTTGCC AACCGGGACA TCACAGACCA CATGGACCGC
    CTGCCTCGCG ACATCGCGCA GGAGCGTATG CACCACGACA TCGTGCGGCT GCTGGACGAG
    TACAGCCTGG TGCGCAGCCC CCCGCTGCAT GGCGCCACCC TGGGCGGCAC GCCCACCCTG
    TCGCCCCCGC TCTGCTCGCC CAACGGCTAC CTGGGCAACC TCAAGCCCCC CATGCAGGGC
    AAGAAGGCCC GCAAGCCCAG CACCAAGGGC CTGGCCTGCG GCGGCAAGGA GCCCAAGGAC
    CTCAAGGCTC GGAGGAAGAA GTCCCAAGAT GGCAAGGGCT GTCTGCTGGA CAGCGGGAGC
    GTGATGTCGC CCGTGGACTC CCTCGAGTCC CCCCACGGCT ACCTGTCAGA CGTGGCCTCC
    CCACCCCTCC TGCCCTCCCC TTTCCAGCCG TCTCCCTCCG TGCCCCTCAA CCACCTGCCC
    GGGATGCCCG AGACCCACCT GGGCGTCAGC CACCTGAGCG TGGCGGCCAA GCCCGAGATG
    GCGGTGCTGA GCGGGGGCAG CCGGCTGGCC TTCGAGGCAG GGCCACCACG CCTCTCCCAC
    CTGCCCGTGG CCTCCAGCAC CAGCACGATC CTGGGTTCGG GCGGCAGTGG GGGCAGTGGG
    GCTGTGAATT TCACCGTGGG TGGAGCTGCG GGCTTGAATG GCCAGTGCGA GTGGCTGTCC
    CGGCTGCAGA ACGGCCTGGT GCCCAACCAG TACAACCCGC TGCGAGGGGG CGTGACGCCG
    GGCACCCTGA GCACGCAGGC TGCTGGCCTC CAGCATGGCA CGGTGGGCCC GCTGCACGCC
    CCCGCCCTGT CCCAGGTGAT GACCTACCAG GCCCTGCCCA GCACGCGGCT GGCCTCCCAG
    CCTCACCTGG TGCAGCCGCA GCAGAACTTA CAGATGCAGC CGCCGAGCAT GCCGCCGCAG
    CCAAACCTGC AGCCGCACCT CGGCGTAAGC TCGGCCGCCA GCGGCCACCT GGGCCGGAGC
    TTCCTGGGAG GGGAGCTGAG CCAGGCGGAC ATGCAGCCGC TGGGGCCCGG CAACCTGGCG
    GCGCACACCG TCCTGCCGCA GGACGGCCAG GTCCTGCCCA CATCGCTGCC GTCCACGCTG
    GCCCCGCCCA CGATGGCCCC GCCCATGACC ACCGCCCAGT TCCTGACGCC CCCCTCCCAG
    CACAGCTACT CGTCCTCCCC CGTGGACAAC ACCCCCAGCC ACCAGCTACA GGTGCCCGAG
    CACCCCTTCC TCACCCCGTC CCCGGAGTCC CCCGACCAGT GGTCCAGCTC CTCGCCACAT
    TCCAACATCT CGGATTGGTC CGAGGGCATC TCCAGCCCGC CCACCAGCGT GCCGTCCCAG
    ATCGCCCACG TCCCGGAGGC ATTCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321035.1
    CDS249..6998
    Translation

    Target ORF information:

    RefSeq Version XM_015465549.1
    Organism Bos taurus(cattle)
    Definition Bos taurus notch 1 (NOTCH1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465549.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGCAGGCCC TGCCCACAGG TCAGTACTGC ACAGAGGACG TGGACGAGTG CCAGCTCATG 
    CCCAACGCCT GCCAGAACGG CGGGACCTGC CACAACACCC ACGGCGGCTA CAACTGCGTG
    TGCGTCAACG GCTGGACGGG CGAGGACTGC AGCGAGAACA TCGACGACTG CGCCAGCGCC
    TCCTGCTTCC AGGGCGCCAC CTGCCACGAC CGCGTCGCCT CCTTCTACTG CGAGTGTCCC
    CACGGCCGCA CGGGTCTGCT GTGCCACCTG AACGATGCCT GCATCAGCAA CCCCTGCAAC
    GAGGGCTCCA ACTGCGACAC CAACCCCGTC AACGGCAAGG CCATCTGCAC CTGCCCCTCG
    GGGTACACGG GGCCGGCCTG CAGCCAGGAT GTGGACGAGT GCTCGCTGGG CGCCAACCCC
    TGTGAGCACG CGGGCAAGTG CATCAACACG CTGGGCTCGT TCGAGTGCCA GTGTCTGCAG
    GGCTACACCG GCCCGCGCTG TGAGATCGAC GTCAACGAGT GTGTATCGAA CCCGTGTCAG
    AACGACGCCA CTTGTCTGGA TCAGATCGGG GAGTTCCAGT GTATCTGCAT GCCTGGCTAC
    GAGGGCTTGC ACTGCGAGGT GAACACGGAC GAGTGTGCCA GCAGCCCCTG CCTGCAGAAC
    GGCCGCTGCC TGGACAAGAT CAACGAGTTC GTGTGCGAGT GCCCCACTGG CTTCACCGGG
    CACTTGTGCC AGTATGACGT GGACGAGTGT GCAAGCACAC CCTGCAAGAA CGGCGCTAAG
    TGCCTGGACG GGCCCAACAC CTACACCTGC GTGTGCACAG AAGGCTACAC GGGGCCCCAC
    TGCGAGGTGG ACATCGACGA GTGTGACCCC GACCCCTGCC ACTACGGGTC CTGCAAGGAC
    GGCGTGGCCA CCTTCACCTG CCTGTGCCAG CCGGGCTACA CGGGCCACCA CTGTGAGAGC
    AACATTAACG AGTGCCACAG CCAGCCCTGC CGCCACGGGG GCACCTGCCA GGACCGCGAC
    AACGCCTATC TCTGCTTCTG CCTCAAGGGG ACCACAGGAC CCAACTGCGA GATCAACCTG
    GATGACTGCG CAAGCAACCC TTGCGACTCC GGCACGTGTC TGGACAAGAT TGACGGCTAC
    GAGTGTGCCT GTGAGCCAGG CTACACAGGG AGTATGTGTA ACATCAACAT TGACGAGTGT
    GCGGACAGCC CTTGCCACAA CGGGGGCACC TGCGAGGATG GCATCAACGG CTTCACCTGT
    CGCTGCCCCG AGGGCTACCA CGACCCCACC TGCCTGTCTG AGGTCAACGA GTGCAGCAGC
    AACCCCTGCA TCCATGGGGC CTGCCGCGAC AGCCTCAACG GGTACAAGTG TGACTGTGAC
    CCCGGGTGGA GTGGGGCCAA CTGTGACGTC AACAACGACG AGTGTGAGTC GAACCCCTGC
    ATCAACGGGG GCACCTGCAA GGACATGACC AGCGGCTACG TGTGCGCCTG CCGCGAGGGC
    TTCAGTGGGC CCAACTGCCA GACCAACATC AACGAGTGCG CATCCAACCC ATGTCTGAAC
    CAGGGCACGT GTATCGATGA CGTGGCGGGG TACAAATGCA ACTGCCTCTT GCCCTACACA
    GGGGCCACGT GTGAGGTGGT GCTGGCCCCA TGTGCCCCCG GGCCCTGCAG AAACGGCGGT
    GAGTGTCGGG AGTCAGAGGA CTATGAGAGC TTCTCCTGTG CCTGCCCCGC GGGCTGGCAG
    GGGCAGACCT GCGAGATCGA CATCAACGAG TGCGTGAAGA GCCCGTGCCG CGCGGGCGCC
    TCCTGCCAGA ACACCAACGG CAGCTACCGC TGCCACTGCC AGGCGGGCTA CACGGGGCGC
    AACTGCGAGA CGGACATCGA CGACTGCCGG CCCAACCCGT GCCACAATGG GGGCTCCTGC
    ACAGATGGCA TCAACACGGC CTTCTGCGAC TGCCTGCCCG GCTTCCAGGG CGCCTTCTGC
    GAGGAGGACA TCAACGAGTG CGCCAGCAGC CCCTGCCGCA ACGGCGCCAA CTGCACCGAC
    TGTGTGGACA GCTACACCTG CACCTGCCCC ACGGGCTTCA GCGGCATCCA CTGCGAGAAC
    AACACGCCCG ACTGCACGGA GAGCTCCTGC TTCAACGGCG GTACCTGCGT GGATGGCATC
    AACTCCTTCA CCTGCCTGTG TCCACCTGGC TTCACGGGCA GCTACTGCCA ACATGACGTC
    AACGAGTGTG ACTCTCGGCC CTGCCTGCAC GGCGGCACCT GCCACGACAG CTACGGCACC
    TACACGTGCA CCTGCCCGCA GGGCTACACA GGCCTCAACT GCCAGACCCT CGTGCGCTGG
    TGCGACTCGT CCCCCTGCAA GAATGACGGC CGGTGCTGGC AGACCAACGC GCTCTACCGC
    TGTGAGTGCC ACAGCGGCTG GACCGGCCTC TACTGTGACG TGCCCAGCGT CTCCTGCGAG
    GTGGCCGCGC GGCAGCAAGG CGTCAACGTG ACCCACTTGT GCCGGAACGG GGGCCTCTGC
    ATGAATGCGG GCAACACACA CCGCTGCCAC TGCCAGGCCG GCTACACGGG CAGCTACTGC
    GAGGAGCAGG TGGACGAGTG CTCGCCCAGC CCCTGCCAGA ACGGGGCCAC CTGCACCGAC
    TACCCCGGCG GCTACTCCTG TGAGTGTGTG GCCGGCTACC ACGGGGTGAA CTGCTCCGAG
    GAGGTGAACG AGTGCCTGTC CCAGCCCTGC CGGAACGGAG GCACCTGCAT CGACCTCACC
    AACACCTACA AGTGTTCCTG CCCCCGCGGC ACACAGGGCG TGCACTGCGA GATCAACGTG
    GACGACTGCA ACCCCCCCAT CGACCCCGTG TCCCGGGGCC CCAAGTGCTT CAACAACGGC
    ACCTGCGTGG ACCAGGTGGG CGGCTACAGC TGCAGCTGCC CGCCCGGCTT CGTGGGTGAG
    CGCTGCGAGG GGGATGTCAA CGAGTGCCTG TCCAACCCCT GCGACGCCCG TGGCACCCAG
    AACTGCGTGC AGCACGTCAA CGCCTTCCAC TGCGAGTGCC GCGCCGGCCA CACCGGACGC
    CGCTGCGAGT CAGTCATCAA CGGCTGCAAA GACAGGCCCT GTAAGAATGG GGGTAGCTGT
    GCAGTGGCCT CCAACACGGC CCGTGGGTTC ATCTGCAAGT GCCCAGCGGG CTTCGAGGGC
    GCCACGTGCG AGAACGACGC CCGCAGCTGC GGGAGCCTGC GGTGCCTGAA CGGCGGCACG
    TGCATCGCGG GCCCGCGCAG CCCCACCTGC CTGTGCCTGG GCCCCTTCAC CGGCCCCGAG
    TGCCAGTTCC CGGCCAGCAG CCCCTGCGTG GGCGGCAACC CCTGCTACAA CCAGGGCGTC
    TGTGAGCCCA CCGCCGAGAG CCCCTTCTAC CGCTGCCGCT GCCCCGCCAA GTTCAACGGG
    CTCCTGTGCC ACATCCTGGA CTACAGCTTC GGGGGCGGCG TGGGCCTCGA CATCCCCCCG
    CCACAGATCG AGGAGACCTG CGAGCTCCCT GGCTGCCGCG AGGAGGCCGG CAACAAGGTC
    TGCAGCCTGC AGTGCAACAG CCACGCGTGC GGCTGGGACG GCGGCGACTG CTCCCTCGAC
    TTCGACGACC CTTGGCAGAA CTGCACGCAG TCCCTGCAGT GCTGGAAGTA CTTCAGCAAC
    GGCCGCTGCG ACAGCCAGTG CAACTCGGCC GGCTGCCTCT TCGACGGCTT CGACTGTCAG
    CGCGCAGAAG GCCAGTGCAA CCCCCTGTAC GACCAGTACT GCAAGGACCA CTTCAGGGAC
    GGGCACTGCG ACCAGGGCTG CAACAGTGCG GAGTGCGAGT GGGACGGGCT GGACTGTGCG
    GAGCATGTGC CCGAGCGGCT GGCGGCCGGC ACGCTGGTGC TGGTGGTGCT CATGCCGCCC
    GAGCAGCTGC GCAACCGCTC CCTGCACTTC CTGCGCGAGC TCAGCCGCCT GCTGCACACC
    AATGTGGTCT TCAAGCGTGA TGCCAGTGGC CAGCAGATGA TCTTCCCCTA CTACGGCCGC
    GGGAGGAGCT GCACAAGCAC CCCGTCAGGC TCCGTGGACG GTGGGGCCCC ACTGCTCCTG
    GGCGGCGCGG GCAGACGCCA GCGCAGGGAG CTGGACCCCA TGGACATCCG CGGGTCCATC
    GTCTACCTGG AGATCGACAA CCGGCAGTGC GTCCAGTCAT CCTCCCAGTG CTTCCAGAGC
    GCCACGGACG TGGCCGCCTT CTTGGGCGCG CTGGCCTCGC TGGGCAGCCT TAACATCCCC
    TACAAGATCG AGGCTGTGCA GAGTGAGACG GTGGAGCCGC CCCCGCCGCC GCCGCTGCAC
    TTCATGTATG TGGCTGTGGT GGCCTTCGTG TTGCTGTTCT TCGTGGGCTG CGGGGTGCTG
    CTGTCGCGGA AGCGCCGGCG GCAGCATGGC CAGCTCTGGT TCCCTGAGGG CTTCAAGGTG
    TCGGAGGCCA GCAAGAAGAA GCGGCGCGAG CCCCTCGGCG AGGACTCCGT GGGCCTCAAG
    CCCCTGAAGA ACTCCTCGGA CGGCGCCCTC ATGGACGACA ACCAGAACGA GTGGGGCGAC
    GAGGGCCTGG AGGCCAAGAA GTTCCGGTTC GAGGAGCCCG TGGTCCTCCC TGACCTGGAC
    GACCAGACGG ACCACCGGCA GTGGACCCAG CAGCACCTGG ACGCCGCCGA CCTGCGCGTG
    TCTGCCATGG CCCCCACGCC TCCCCAGGGC GAGGCGGATG CCGACTGCAT GGACGTGAAC
    GTCCGCGGGC CGGACGGCTT CACGCCCCTC ATGATCGCCT CCTGCAGCGG AGGGGGCCTA
    GAGACTGGCA ACAGCGAGGA GGAGGAGGAC GCGCCCGCGG TCATCTCGGA CTTCATCTAC
    CAGGGCGCCA GCCTGCACAA CCAGACGGAC CGCACCGGCG AGACCGCCCT GCACCTGGCC
    GCCCGCTACT CGCGCTCCGA CGCGGCCAAG CGCCTGCTGG AGGCCAGCGC GGACGCCAAC
    ATACAGGACA ACATGGGGCG CACCCCGCTG CACGCGGCCG TGTCTGCCGA CGCACAGGGC
    GTTTTCCAGA TCCTGATCCG GAACCGAGCC ACCGACCTGG ATGCCCGCAT GCACGACGGC
    ACGACCCCGC TGATCCTCGC TGCCCGCCTG GCCGTGGAGG GCATGCTGGA GGACCTCATC
    AACTCTCACG CCGACGTCAA CGCTGTGGAT GACCTGGGCA AGTCCGCCCT GCACTGGGCA
    GCCGCGGTGA ACAACGTGGA GGCTGCTGTC GTGCTGCTGA AGAACGGGGC CAACAAGGAC
    ATGCAGAACA ACAAGGAGGA GACGCCCTTG TTCCTGGCCG CCCGGGAGGG CAGCTACGAG
    ACCGCCAAGG TGCTGCTGGA CCATTTTGCC AACCGGGACA TCACAGACCA CATGGACCGC
    CTGCCTCGCG ACATCGCGCA GGAGCGTATG CACCACGACA TCGTGCGGCT GCTGGACGAG
    TACAGCCTGG TGCGCAGCCC CCCGCTGCAT GGCGCCACCC TGGGCGGCAC GCCCACCCTG
    TCGCCCCCGC TCTGCTCGCC CAACGGCTAC CTGGGCAACC TCAAGCCCCC CATGCAGGGC
    AAGAAGGCCC GCAAGCCCAG CACCAAGGGC CTGGCCTGCG GCGGCAAGGA GCCCAAGGAC
    CTCAAGGCTC GGAGGAAGAA GTCCCAAGAT GGCAAGGGCT GTCTGCTGGA CAGCGGGAGC
    GTGATGTCGC CCGTGGACTC CCTCGAGTCC CCCCACGGCT ACCTGTCAGA CGTGGCCTCC
    CCACCCCTCC TGCCCTCCCC TTTCCAGCCG TCTCCCTCCG TGCCCCTCAA CCACCTGCCC
    GGGATGCCCG AGACCCACCT GGGCGTCAGC CACCTGAGCG TGGCGGCCAA GCCCGAGATG
    GCGGTGCTGA GCGGGGGCAG CCGGCTGGCC TTCGAGGCAG GGCCACCACG CCTCTCCCAC
    CTGCCCGTGG CCTCCAGCAC CAGCACGATC CTGGGTTCGG GCGGCAGTGG GGGCAGTGGG
    GCTGTGAATT TCACCGTGGG TGGAGCTGCG GGCTTGAATG GCCAGTGCGA GTGGCTGTCC
    CGGCTGCAGA ACGGCCTGGT GCCCAACCAG TACAACCCGC TGCGAGGGGG CGTGACGCCG
    GGCACCCTGA GCACGCAGGC TGCTGGCCTC CAGCATGGCA CGGTGGGCCC GCTGCACGCC
    CCCGCCCTGT CCCAGGTGAT GACCTACCAG GCCCTGCCCA GCACGCGGCT GGCCTCCCAG
    CCTCACCTGG TGCAGCCGCA GCAGAACTTA CAGATGCAGC CGCCGAGCAT GCCGCCGCAG
    CCAAACCTGC AGCCGCACCT CGGCGTAAGC TCGGCCGCCA GCGGCCACCT GGGCCGGAGC
    TTCCTGGGAG GGGAGCTGAG CCAGGCGGAC ATGCAGCCGC TGGGGCCCGG CAACCTGGCG
    GCGCACACCG TCCTGCCGCA GGACGGCCAG GTCCTGCCCA CATCGCTGCC GTCCACGCTG
    GCCCCGCCCA CGATGGCCCC GCCCATGACC ACCGCCCAGT TCCTGACGCC CCCCTCCCAG
    CACAGCTACT CGTCCTCCCC CGTGGACAAC ACCCCCAGCC ACCAGCTACA GGTGCCCGAG
    CACCCCTTCC TCACCCCGTC CCCGGAGTCC CCCGACCAGT GGTCCAGCTC CTCGCCACAT
    TCCAACATCT CGGATTGGTC CGAGGGCATC TCCAGCCCGC CCACCAGCGT GCCGTCCCAG
    ATCGCCCACG TCCCGGAGGC ATTCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94698
    Clone ID Related Accession (Same CDS sequence) XM_015465550.1
    Accession Version XM_015465550.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neurogenic locus notch homolog protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645923.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCGCCGC TCCTGGCGCC CCTGCTTTGC CTGGCGTTGC TGCCTGCGCT CGCCGCACGA 
    GGCCTGCGCT GCTCCCAGCC CGGTGAGACC TGCTTGAACG GTGGGAAGTG TGAAGTGTTC
    CCCAACGGCA CCGAGGCCTG CATCTGCGGC GGGGCCTTCG CGGGCCAGCA ATGCCAGGCC
    CCCAACCCCT GCCTCAGCGC CCCCTGCAAG AACGGCGGGA CGTGCCACAC GACGGAGCGA
    GAAGGCCTGG TGGACTATGT CTGCGGCTGC CGCCTGGGCT TCTCGGGGCC ACTGTGCCTG
    ACGCCCCGCG ACCACGCTTG CCTCGCCAGC CCCTGCCTCA ACGGTGGCAC CTGTGATCTG
    CTCACGCTCA CTGAGTACAA GTGCCTGTGC ACCCCGGGCT GGTCAGGGAA GACGTGCCAG
    CAGGCCGACC CCTGCGCCTC CAACCCCTGT GCCAACGGTG GCCAGTGCCT GCCCTTTGAG
    GCGTCGTATA TCTGCCACTG CCCGCCCGGC TTCCACGGCC CCACGTGCAG GCAGGACGTC
    AACGAGTGCA GCCAGAGCCC CGGGCTCTGC CACCACGGCG GCACCTGCCT CAACGAGGTC
    GGCTCCTACC GCTGCGTCTG CCGCCCCACC CACACCGGCC CCCACTGCGA GCTGCCCTAC
    GTGCCCTGCA GTCCCTCCCC CTGCCAGAAC GGGGGCACCT GCCGCCCCAC GGGGGACACC
    ACCCACGAGT GCGCCTGCCT GCCAGGCTTC ACTGGCCAGA ACTGTGAGGA AAACATTGAC
    GACTGTCCGG GGAACAGCTG CAAGAACGGA GGCGCCTGTG TGGACGGCGT GAACACCTAC
    AACTGCCGCT GCCCCCCGGA GTGGACAGGT CAGTACTGCA CAGAGGACGT GGACGAGTGC
    CAGCTCATGC CCAACGCCTG CCAGAACGGC GGGACCTGCC ACAACACCCA CGGCGGCTAC
    AACTGCGTGT GCGTCAACGG CTGGACGGGC GAGGACTGCA GCGAGAACAT CGACGACTGC
    GCCAGCGCCT CCTGCTTCCA GGGCGCCACC TGCCACGACC GCGTCGCCTC CTTCTACTGC
    GAGTGTCCCC ACGGCCGCAC GGGTCTGCTG TGCCACCTGA ACGATGCCTG CATCAGCAAC
    CCCTGCAACG AGGGCTCCAA CTGCGACACC AACCCCGTCA ACGGCAAGGC CATCTGCACC
    TGCCCCTCGG GGTACACGGG GCCGGCCTGC AGCCAGGATG TGGACGAGTG CTCGCTGGGC
    GCCAACCCCT GTGAGCACGC GGGCAAGTGC ATCAACACGC TGGGCTCGTT CGAGTGCCAG
    TGTCTGCAGG GCTACACCGG CCCGCGCTGT GAGATCGACG TCAACGAGTG TGTATCGAAC
    CCGTGTCAGA ACGACGCCAC TTGTCTGGAT CAGATCGGGG AGTTCCAGTG TATCTGCATG
    CCTGGCTACG AGGGCTTGCA CTGCGAGGTG AACACGGACG AGTGTGCCAG CAGCCCCTGC
    CTGCAGAACG GCCGCTGCCT GGACAAGATC AACGAGTTCG TGTGCGAGTG CCCCACTGGC
    TTCACCGGGC ACTTGTGCCA GTATGACGTG GACGAGTGTG CAAGCACACC CTGCAAGAAC
    GGCGCTAAGT GCCTGGACGG GCCCAACACC TACACCTGCG TGTGCACAGA AGGCTACACG
    GGGCCCCACT GCGAGGTGGA CATCGACGAG TGTGACCCCG ACCCCTGCCA CTACGGGTCC
    TGCAAGGACG GCGTGGCCAC CTTCACCTGC CTGTGCCAGC CGGGCTACAC GGGCCACCAC
    TGTGAGAGCA ACATTAACGA GTGCCACAGC CAGCCCTGCC GCCACGGGGG CACCTGCCAG
    GACCGCGACA ACGCCTATCT CTGCTTCTGC CTCAAGGGGA CCACAGGACC CAACTGCGAG
    ATCAACCTGG ATGACTGCGC AAGCAACCCT TGCGACTCCG GCACGTGTCT GGACAAGATT
    GACGGCTACG AGTGTGCCTG TGAGCCAGGC TACACAGGGA GTATGTGTAA CATCAACATT
    GACGAGTGTG CGGACAGCCC TTGCCACAAC GGGGGCACCT GCGAGGATGG CATCAACGGC
    TTCACCTGTC GCTGCCCCGA GGGCTACCAC GACCCCACCT GCCTGTCTGA GGTCAACGAG
    TGCAGCAGCA ACCCCTGCAT CCATGGGGCC TGCCGCGACA GCCTCAACGG GTACAAGTGT
    GACTGTGACC CCGGGTGGAG TGGGGCCAAC TGTGACGTCA ACAACGACGA GTGTGAGTCG
    AACCCCTGCA TCAACGGGGG CACCTGCAAG GACATGACCA GCGGCTACGT GTGCGCCTGC
    CGCGAGGGCT TCAGTGGGCC CAACTGCCAG ACCAACATCA ACGAGTGCGC ATCCAACCCA
    TGTCTGAACC AGGGCACGTG TATCGATGAC GTGGCGGGGT ACAAATGCAA CTGCCTCTTG
    CCCTACACAG GGGCCACGTG TGAGGTGGTG CTGGCCCCAT GTGCCCCCGG GCCCTGCAGA
    AACGGCGGTG AGTGTCGGGA GTCAGAGGAC TATGAGAGCT TCTCCTGTGC CTGCCCCGCG
    GGCTGGCAGG GGCAGACCTG CGAGATCGAC ATCAACGAGT GCGTGAAGAG CCCGTGCCGC
    GCGGGCGCCT CCTGCCAGAA CACCAACGGC AGCTACCGCT GCCACTGCCA GGCGGGCTAC
    ACGGGGCGCA ACTGCGAGAC GGACATCGAC GACTGCCGGC CCAACCCGTG CCACAATGGG
    GGCTCCTGCA CAGATGGCAT CAACACGGCC TTCTGCGACT GCCTGCCCGG CTTCCAGGGC
    GCCTTCTGCG AGGAGGACAT CAACGAGTGC GCCAGCAGCC CCTGCCGCAA CGGCGCCAAC
    TGCACCGACT GTGTGGACAG CTACACCTGC ACCTGCCCCA CGGGCTTCAG CGGCATCCAC
    TGCGAGAACA ACACGCCCGA CTGCACGGAG AGCTCCTGCT TCAACGGCGG TACCTGCGTG
    GATGGCATCA ACTCCTTCAC CTGCCTGTGT CCACCTGGCT TCACGGGCAG CTACTGCCAA
    CATGACGTCA ACGAGTGTGA CTCTCGGCCC TGCCTGCACG GCGGCACCTG CCACGACAGC
    TACGGCACCT ACACGTGCAC CTGCCCGCAG GGCTACACAG GCCTCAACTG CCAGACCCTC
    GTGCGCTGGT GCGACTCGTC CCCCTGCAAG AATGACGGCC GGTGCTGGCA GACCAACGCG
    CTCTACCGCT GTGAGTGCCA CAGCGGCTGG ACCGGCCTCT ACTGTGACGT GCCCAGCGTC
    TCCTGCGAGG TGGCCGCGCG GCAGCAAGGC GTCAACGTGA CCCACTTGTG CCGGAACGGG
    GGCCTCTGCA TGAATGCGGG CAACACACAC CGCTGCCACT GCCAGGCCGG CTACACGGGC
    AGCTACTGCG AGGAGCAGGT GGACGAGTGC TCGCCCAGCC CCTGCCAGAA CGGGGCCACC
    TGCACCGACT ACCCCGGCGG CTACTCCTGT GAGTGTGTGG CCGGCTACCA CGGGGTGAAC
    TGCTCCGAGG AGGTGAACGA GTGCCTGTCC CAGCCCTGCC GGAACGGAGG CACCTGCATC
    GACCTCACCA ACACCTACAA GTGTTCCTGC CCCCGCGGCA CACAGGGCGT GCACTGCGAG
    ATCAACGTGG ACGACTGCAA CCCCCCCATC GACCCCGTGT CCCGGGGCCC CAAGTGCTTC
    AACAACGGCA CCTGCGTGGA CCAGGTGGGC GGCTACAGCT GCAGCTGCCC GCCCGGCTTC
    GTGGGTGAGC GCTGCGAGGG GGATGTCAAC GAGTGCCTGT CCAACCCCTG CGACGCCCGT
    GGCACCCAGA ACTGCGTGCA GCACGTCAAC GCCTTCCACT GCGAGTGCCG CGCCGGCCAC
    ACCGGACGCC GCTGCGAGTC AGTCATCAAC GGCTGCAAAG ACAGGCCCTG TAAGAATGGG
    GGTAGCTGTG CAGTGGCCTC CAACACGGCC CGTGGGTTCA TCTGCAAGTG CCCAGCGGGC
    TTCGAGGGCG CCACGTGCGA GAACGACGCC CGCAGCTGCG GGAGCCTGCG GTGCCTGAAC
    GGCGGCACGT GCATCGCGGG CCCGCGCAGC CCCACCTGCC TGTGCCTGGG CCCCTTCACC
    GGCCCCGAGT GCCAGTTCCC GGCCAGCAGC CCCTGCGTGG GCGGCAACCC CTGCTACAAC
    CAGGGCGTCT GTGAGCCCAC CGCCGAGAGC CCCTTCTACC GCTGCCGCTG CCCCGCCAAG
    TTCAACGGGC TCCTGTGCCA CATCCTGGAC TACAGCTTCG GGGGCGGCGT GGGCCTCGAC
    ATCCCCCCGC CACAGATCGA GGAGACCTGC GAGCTCCCTG GCTGCCGCGA GGAGGCCGGC
    AACAAGGTCT GCAGCCTGCA GTGCAACAGC CACGCGTGCG GCTGGGACGG CGGCGACTGC
    TCCCTCGACT TCGACGACCC TTGGCAGAAC TGCACGCAGT CCCTGCAGTG CTGGAAGTAC
    TTCAGCAACG GCCGCTGCGA CAGCCAGTGC AACTCGGCCG GCTGCCTCTT CGACGGCTTC
    GACTGTCAGC GCGCAGAAGG CCAGTGCAAC CCCCTGTACG ACCAGTACTG CAAGGACCAC
    TTCAGGGACG GGCACTGCGA CCAGGGCTGC AACAGTGCGG AGTGCGAGTG GGACGGGCTG
    GACTGTGCGG AGCATGTGCC CGAGCGGCTG GCGGCCGGCA CGCTGGTGCT GGTGGTGCTC
    ATGCCGCCCG AGCAGCTGCG CAACCGCTCC CTGCACTTCC TGCGCGAGCT CAGCCGCCTG
    CTGCACACCA ATGTGGTCTT CAAGCGTGAT GCCAGTGGCC AGCAGATGAT CTTCCCCTAC
    TACGGCCGCG GGAGGAGCTG CACAAGCACC CCGTCAGGCT CCGTGGACGG TGGGGCCCCA
    CTGCTCCTGG GCGGCGCGGG CAGACGCCAG CGCAGGGAGC TGGACCCCAT GGACATCCGC
    GGGTCCATCG TCTACCTGGA GATCGACAAC CGGCAGTGCG TCCAGTCATC CTCCCAGTGC
    TTCCAGAGCG CCACGGACGT GGCCGCCTTC TTGGGCGCGC TGGCCTCGCT GGGCAGCCTT
    AACATCCCCT ACAAGATCGA GGCTGTGCAG AGTGAGTGGC CAGCCCTGCT CCCCCAGCTG
    CACTCCCACG GGAGCTCTGG ACTGTCCTGG GAGGGGTTGT GCATGGGAGA CGGTCTCCCA
    GCTGCACCCC CACCCCGGGA TGCTCTTGTT CTTTGCGCCA GTTGCCACCC ACGGGGGCGT
    GTGTCACGCC ATCCAGAGTT CGGGGAGCGC TTTTCTGGGG TTTCCCACCG GGCACGCTCT
    CTCTCCCGTA GCAACAGCTG TCAGCGAGGC TGGCCCAAGG GAGTCCACAC ATCCACCAGC
    AAATGTTGGA GGGGAGACTT TTCTCGCCCC ACCCCTTCCT CCCGATTTTT CTTTGTTGGG
    TATTATTTCA AAATCATTAC AGTTTTTTTT TTTTTTTTAA AGAGAGAGAA AGCAAAGAAT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321036.1
    CDS255..5777
    Translation

    Target ORF information:

    RefSeq Version XM_015465550.1
    Organism Bos taurus(cattle)
    Definition Bos taurus notch 1 (NOTCH1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465550.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCGCCGC TCCTGGCGCC CCTGCTTTGC CTGGCGTTGC TGCCTGCGCT CGCCGCACGA 
    GGCCTGCGCT GCTCCCAGCC CGGTGAGACC TGCTTGAACG GTGGGAAGTG TGAAGTGTTC
    CCCAACGGCA CCGAGGCCTG CATCTGCGGC GGGGCCTTCG CGGGCCAGCA ATGCCAGGCC
    CCCAACCCCT GCCTCAGCGC CCCCTGCAAG AACGGCGGGA CGTGCCACAC GACGGAGCGA
    GAAGGCCTGG TGGACTATGT CTGCGGCTGC CGCCTGGGCT TCTCGGGGCC ACTGTGCCTG
    ACGCCCCGCG ACCACGCTTG CCTCGCCAGC CCCTGCCTCA ACGGTGGCAC CTGTGATCTG
    CTCACGCTCA CTGAGTACAA GTGCCTGTGC ACCCCGGGCT GGTCAGGGAA GACGTGCCAG
    CAGGCCGACC CCTGCGCCTC CAACCCCTGT GCCAACGGTG GCCAGTGCCT GCCCTTTGAG
    GCGTCGTATA TCTGCCACTG CCCGCCCGGC TTCCACGGCC CCACGTGCAG GCAGGACGTC
    AACGAGTGCA GCCAGAGCCC CGGGCTCTGC CACCACGGCG GCACCTGCCT CAACGAGGTC
    GGCTCCTACC GCTGCGTCTG CCGCCCCACC CACACCGGCC CCCACTGCGA GCTGCCCTAC
    GTGCCCTGCA GTCCCTCCCC CTGCCAGAAC GGGGGCACCT GCCGCCCCAC GGGGGACACC
    ACCCACGAGT GCGCCTGCCT GCCAGGCTTC ACTGGCCAGA ACTGTGAGGA AAACATTGAC
    GACTGTCCGG GGAACAGCTG CAAGAACGGA GGCGCCTGTG TGGACGGCGT GAACACCTAC
    AACTGCCGCT GCCCCCCGGA GTGGACAGGT CAGTACTGCA CAGAGGACGT GGACGAGTGC
    CAGCTCATGC CCAACGCCTG CCAGAACGGC GGGACCTGCC ACAACACCCA CGGCGGCTAC
    AACTGCGTGT GCGTCAACGG CTGGACGGGC GAGGACTGCA GCGAGAACAT CGACGACTGC
    GCCAGCGCCT CCTGCTTCCA GGGCGCCACC TGCCACGACC GCGTCGCCTC CTTCTACTGC
    GAGTGTCCCC ACGGCCGCAC GGGTCTGCTG TGCCACCTGA ACGATGCCTG CATCAGCAAC
    CCCTGCAACG AGGGCTCCAA CTGCGACACC AACCCCGTCA ACGGCAAGGC CATCTGCACC
    TGCCCCTCGG GGTACACGGG GCCGGCCTGC AGCCAGGATG TGGACGAGTG CTCGCTGGGC
    GCCAACCCCT GTGAGCACGC GGGCAAGTGC ATCAACACGC TGGGCTCGTT CGAGTGCCAG
    TGTCTGCAGG GCTACACCGG CCCGCGCTGT GAGATCGACG TCAACGAGTG TGTATCGAAC
    CCGTGTCAGA ACGACGCCAC TTGTCTGGAT CAGATCGGGG AGTTCCAGTG TATCTGCATG
    CCTGGCTACG AGGGCTTGCA CTGCGAGGTG AACACGGACG AGTGTGCCAG CAGCCCCTGC
    CTGCAGAACG GCCGCTGCCT GGACAAGATC AACGAGTTCG TGTGCGAGTG CCCCACTGGC
    TTCACCGGGC ACTTGTGCCA GTATGACGTG GACGAGTGTG CAAGCACACC CTGCAAGAAC
    GGCGCTAAGT GCCTGGACGG GCCCAACACC TACACCTGCG TGTGCACAGA AGGCTACACG
    GGGCCCCACT GCGAGGTGGA CATCGACGAG TGTGACCCCG ACCCCTGCCA CTACGGGTCC
    TGCAAGGACG GCGTGGCCAC CTTCACCTGC CTGTGCCAGC CGGGCTACAC GGGCCACCAC
    TGTGAGAGCA ACATTAACGA GTGCCACAGC CAGCCCTGCC GCCACGGGGG CACCTGCCAG
    GACCGCGACA ACGCCTATCT CTGCTTCTGC CTCAAGGGGA CCACAGGACC CAACTGCGAG
    ATCAACCTGG ATGACTGCGC AAGCAACCCT TGCGACTCCG GCACGTGTCT GGACAAGATT
    GACGGCTACG AGTGTGCCTG TGAGCCAGGC TACACAGGGA GTATGTGTAA CATCAACATT
    GACGAGTGTG CGGACAGCCC TTGCCACAAC GGGGGCACCT GCGAGGATGG CATCAACGGC
    TTCACCTGTC GCTGCCCCGA GGGCTACCAC GACCCCACCT GCCTGTCTGA GGTCAACGAG
    TGCAGCAGCA ACCCCTGCAT CCATGGGGCC TGCCGCGACA GCCTCAACGG GTACAAGTGT
    GACTGTGACC CCGGGTGGAG TGGGGCCAAC TGTGACGTCA ACAACGACGA GTGTGAGTCG
    AACCCCTGCA TCAACGGGGG CACCTGCAAG GACATGACCA GCGGCTACGT GTGCGCCTGC
    CGCGAGGGCT TCAGTGGGCC CAACTGCCAG ACCAACATCA ACGAGTGCGC ATCCAACCCA
    TGTCTGAACC AGGGCACGTG TATCGATGAC GTGGCGGGGT ACAAATGCAA CTGCCTCTTG
    CCCTACACAG GGGCCACGTG TGAGGTGGTG CTGGCCCCAT GTGCCCCCGG GCCCTGCAGA
    AACGGCGGTG AGTGTCGGGA GTCAGAGGAC TATGAGAGCT TCTCCTGTGC CTGCCCCGCG
    GGCTGGCAGG GGCAGACCTG CGAGATCGAC ATCAACGAGT GCGTGAAGAG CCCGTGCCGC
    GCGGGCGCCT CCTGCCAGAA CACCAACGGC AGCTACCGCT GCCACTGCCA GGCGGGCTAC
    ACGGGGCGCA ACTGCGAGAC GGACATCGAC GACTGCCGGC CCAACCCGTG CCACAATGGG
    GGCTCCTGCA CAGATGGCAT CAACACGGCC TTCTGCGACT GCCTGCCCGG CTTCCAGGGC
    GCCTTCTGCG AGGAGGACAT CAACGAGTGC GCCAGCAGCC CCTGCCGCAA CGGCGCCAAC
    TGCACCGACT GTGTGGACAG CTACACCTGC ACCTGCCCCA CGGGCTTCAG CGGCATCCAC
    TGCGAGAACA ACACGCCCGA CTGCACGGAG AGCTCCTGCT TCAACGGCGG TACCTGCGTG
    GATGGCATCA ACTCCTTCAC CTGCCTGTGT CCACCTGGCT TCACGGGCAG CTACTGCCAA
    CATGACGTCA ACGAGTGTGA CTCTCGGCCC TGCCTGCACG GCGGCACCTG CCACGACAGC
    TACGGCACCT ACACGTGCAC CTGCCCGCAG GGCTACACAG GCCTCAACTG CCAGACCCTC
    GTGCGCTGGT GCGACTCGTC CCCCTGCAAG AATGACGGCC GGTGCTGGCA GACCAACGCG
    CTCTACCGCT GTGAGTGCCA CAGCGGCTGG ACCGGCCTCT ACTGTGACGT GCCCAGCGTC
    TCCTGCGAGG TGGCCGCGCG GCAGCAAGGC GTCAACGTGA CCCACTTGTG CCGGAACGGG
    GGCCTCTGCA TGAATGCGGG CAACACACAC CGCTGCCACT GCCAGGCCGG CTACACGGGC
    AGCTACTGCG AGGAGCAGGT GGACGAGTGC TCGCCCAGCC CCTGCCAGAA CGGGGCCACC
    TGCACCGACT ACCCCGGCGG CTACTCCTGT GAGTGTGTGG CCGGCTACCA CGGGGTGAAC
    TGCTCCGAGG AGGTGAACGA GTGCCTGTCC CAGCCCTGCC GGAACGGAGG CACCTGCATC
    GACCTCACCA ACACCTACAA GTGTTCCTGC CCCCGCGGCA CACAGGGCGT GCACTGCGAG
    ATCAACGTGG ACGACTGCAA CCCCCCCATC GACCCCGTGT CCCGGGGCCC CAAGTGCTTC
    AACAACGGCA CCTGCGTGGA CCAGGTGGGC GGCTACAGCT GCAGCTGCCC GCCCGGCTTC
    GTGGGTGAGC GCTGCGAGGG GGATGTCAAC GAGTGCCTGT CCAACCCCTG CGACGCCCGT
    GGCACCCAGA ACTGCGTGCA GCACGTCAAC GCCTTCCACT GCGAGTGCCG CGCCGGCCAC
    ACCGGACGCC GCTGCGAGTC AGTCATCAAC GGCTGCAAAG ACAGGCCCTG TAAGAATGGG
    GGTAGCTGTG CAGTGGCCTC CAACACGGCC CGTGGGTTCA TCTGCAAGTG CCCAGCGGGC
    TTCGAGGGCG CCACGTGCGA GAACGACGCC CGCAGCTGCG GGAGCCTGCG GTGCCTGAAC
    GGCGGCACGT GCATCGCGGG CCCGCGCAGC CCCACCTGCC TGTGCCTGGG CCCCTTCACC
    GGCCCCGAGT GCCAGTTCCC GGCCAGCAGC CCCTGCGTGG GCGGCAACCC CTGCTACAAC
    CAGGGCGTCT GTGAGCCCAC CGCCGAGAGC CCCTTCTACC GCTGCCGCTG CCCCGCCAAG
    TTCAACGGGC TCCTGTGCCA CATCCTGGAC TACAGCTTCG GGGGCGGCGT GGGCCTCGAC
    ATCCCCCCGC CACAGATCGA GGAGACCTGC GAGCTCCCTG GCTGCCGCGA GGAGGCCGGC
    AACAAGGTCT GCAGCCTGCA GTGCAACAGC CACGCGTGCG GCTGGGACGG CGGCGACTGC
    TCCCTCGACT TCGACGACCC TTGGCAGAAC TGCACGCAGT CCCTGCAGTG CTGGAAGTAC
    TTCAGCAACG GCCGCTGCGA CAGCCAGTGC AACTCGGCCG GCTGCCTCTT CGACGGCTTC
    GACTGTCAGC GCGCAGAAGG CCAGTGCAAC CCCCTGTACG ACCAGTACTG CAAGGACCAC
    TTCAGGGACG GGCACTGCGA CCAGGGCTGC AACAGTGCGG AGTGCGAGTG GGACGGGCTG
    GACTGTGCGG AGCATGTGCC CGAGCGGCTG GCGGCCGGCA CGCTGGTGCT GGTGGTGCTC
    ATGCCGCCCG AGCAGCTGCG CAACCGCTCC CTGCACTTCC TGCGCGAGCT CAGCCGCCTG
    CTGCACACCA ATGTGGTCTT CAAGCGTGAT GCCAGTGGCC AGCAGATGAT CTTCCCCTAC
    TACGGCCGCG GGAGGAGCTG CACAAGCACC CCGTCAGGCT CCGTGGACGG TGGGGCCCCA
    CTGCTCCTGG GCGGCGCGGG CAGACGCCAG CGCAGGGAGC TGGACCCCAT GGACATCCGC
    GGGTCCATCG TCTACCTGGA GATCGACAAC CGGCAGTGCG TCCAGTCATC CTCCCAGTGC
    TTCCAGAGCG CCACGGACGT GGCCGCCTTC TTGGGCGCGC TGGCCTCGCT GGGCAGCCTT
    AACATCCCCT ACAAGATCGA GGCTGTGCAG AGTGAGTGGC CAGCCCTGCT CCCCCAGCTG
    CACTCCCACG GGAGCTCTGG ACTGTCCTGG GAGGGGTTGT GCATGGGAGA CGGTCTCCCA
    GCTGCACCCC CACCCCGGGA TGCTCTTGTT CTTTGCGCCA GTTGCCACCC ACGGGGGCGT
    GTGTCACGCC ATCCAGAGTT CGGGGAGCGC TTTTCTGGGG TTTCCCACCG GGCACGCTCT
    CTCTCCCGTA GCAACAGCTG TCAGCGAGGC TGGCCCAAGG GAGTCCACAC ATCCACCAGC
    AAATGTTGGA GGGGAGACTT TTCTCGCCCC ACCCCTTCCT CCCGATTTTT CTTTGTTGGG
    TATTATTTCA AAATCATTAC AGTTTTTTTT TTTTTTTTAA AGAGAGAGAA AGCAAAGAAT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.