ELFN1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ELFN1
Entrez Gene ID	618774
Full Name	extracellular leucine rich repeat and fibronectin type III domain containing 1
General protein information	Preferred Names extracellular leucine rich repeat and fibronectin type III domain containing 1 Names protein ELFN1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001206549.2	NP_001193478.1	protein ELFN1 precursor
XM_005225229.2	XP_005225286.1	protein ELFN1 isoform X1
NM_001206549.1	NP_001193478.1	protein ELFN1 precursor
XM_005225229.3	XP_005225286.2	protein ELFN1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ELFN1	XP_005621225.1
Xenopus tropicalis (tropical clawed frog)	elfn1	XP_002938886.1
	ELFN1	XP_001085829.1
Danio rerio (zebrafish)	LOC562570	XP_005164251.1
Danio rerio (zebrafish)	elfn1b	XP_001920383.1
Bos taurus (cattle)	ELFN1	NP_001193478.1
Mus musculus (house mouse)	Elfn1	NP_780731.1
Rattus norvegicus (Norway rat)	Elfn1	NP_001099383.1
Gallus gallus (chicken)	ELFN1	XP_004945192.1
Homo sapiens (human)	ELFN1	NP_001122108.1
Pan troglodytes (chimpanzee)	ELFN1	XP_001137798.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ELFN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ELFN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa230430	NM_001206549.2 Latest version!	Bos taurus extracellular leucine rich repeat and fibronectin type III domain containing 1 (ELFN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa230430	XM_005225229.3 Latest version!	Bos taurus extracellular leucine rich repeat and fibronectin type III domain containing 1 (ELFN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa01963	NM_001206549.1 Latest version!	Bos taurus extracellular leucine-rich repeat and fibronectin type III domain containing 1 (ELFN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa33325	XM_005225229.2 Latest version!	Bos taurus extracellular leucine-rich repeat and fibronectin type III domain containing 1 (ELFN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ELFN1 ( NM_001206549.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193478.1 Bos taurus extracellular leucine rich repeat and fibronectin type III domain containing 1 (ELFN1), mRNA.

Price & Availability↑

CloneID	OBa230430
Clone ID Related Accession (Same CDS sequence)	NM_001206549.2 , XM_005225229.3
Accession Version	NM_001206549.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-23
Organism	Bos taurus(cattle)
Product	protein ELFN1 precursor
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from NKLS02000025.1. On Apr 24, 2019 this sequence version replaced NM_001206549.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGGCTGGGT GCGGGTGGGA GGCGCCGTGG GTGTGGGTGT GTGTGGCGGC CGCCCTGCTG 
CACGCGGGCG GGCTGGTGCG CGGAGACTGC TGGCTCATCG AGGGTGACAA GGGCTTCGTG 
TGGCTGGCCA TCTGCAGCCA GAACCAGCCG CCCTACGAGG CCATCCCGCA GCAGATCAAC 
AGTACCATCG TGGACCTGCG GCTCAACGAG AACCGCATCC GCAGCGTGCA GTACGCGGCG 
CTGAGCCGCT TCGGCAACCT CACGTACCTG AACCTCACCA AGAACGAGAT CGGCTACATC 
GAGGACGGCG CCTTCTCGGG CCAGTTCAAC CTGCAGGTAC TGCAGCTCGG CTACAACCGG 
CTGCGCAACC TGACGGAGGG CGTGCTGCGC GGCCTGGGCA AGCTCGAGTA CCTGTACCTG 
CAGGCCAACC TTATTGAGGC GGTGGCGCCC GGCGCCTTCT GGGAGTGCCC CAACATCGTC 
AACGTGGACC TGTCCATGAA CCGCATCCAG CGTCTGCACA GCGCCACCTT CGCGGGCCTG 
GCGCGGCTCT CGGTGTGTGA ACTCTACAGC AACCCCTTCT ACTGCTCCTG CGAGCTGCTG 
GGCTTCCTGC GCTGGCTGGC GGCCTTCGCC AACGCTACGC AGGCCCACGA CCGCGTGCAG 
TGCGAGTCCC CGCCGCTCTA CTCCGGCTAC TTCCTGCTGG GCCAGGGCCG CCACGGCCAG 
CGCAGCATCC TGGGCAAGCT GCAGTCTGTG TGCACTGACG GCCCGTATGC GGCGGAGACC 
CGCCCGGCGC CCGGCCGCCC CCCGCCCGGC CGCTCGCCGC CGCCGCCCCC CGTGCCGCCC 
GCGGAGCCCA GCGAGGCCCC CTGCGCTGAG GACGAGTGCT TCTCCGGCGA CGGCACCACG 
CCGCTGGTGG CGCTGCCCAC GCTGGCCCCG CAGGCCGAGG CCCGCCCGCT CATCAAGGTC 
AAGCAGCTGA CGCAGAACTC GGCCACCATC ACGGTGCAGC TGCCCAGCCC CTTCACCCGC 
ATGTACACGC TGGAGCACTT CAACAACAGC AAGTCGTCCA CCGTGTCCAG GCTGACCCGG 
GCCCAGGAGG AGATCCGCCT GACCAACCTG TACGCGCTCA CCAACTACAC CTATTGTGTG 
GTGTCCAGCA GCTCGGGCCT GCACCACAAC CACACCTGCC TCACCATCTG CCTGCCGCAG 
CCGCCTAGCC CGCCGGGCCC CGTGCCCAGC CCCTCCACGG CCACCCACTA CATCATGACC 
GTCCTGGGCT GCCTCTTCGG CATGGTGGTG GTGCTGGGCG CCGTCTACTA CTGCCTGCGG 
CGGCGGCGGC GGCGGGAGGG CAAGCAGAAG CAGGCGGCGG CAGCGGCCGG CAGCCTCAAG 
AAGACCATCA TCGAGCTCAA GTACGGGCCT GAGCTGGAGG CGCCAGGCCT GGCCCCGCTG 
TCCCAGGGCC CGCTGCTGGG CCCCGAGGCG GTGACCCGCA TCCCATACCT GCCCGCCGCC 
CCCGGGGAGG TGGAGCAGTA CCAGCTGGCG GAGGGCAGCG GGACACCCAA GGGCAGCAAG 
GGCGGCTACA TGGAGGTGCG TGCGGCAGAG CAGGCAGAAC GCAGGGAGGG GGAGCAGGGC 
CGGCCGGGCC CCGACAGCCA GAGCTCGGTG GCTGAGATCT CCACCATCGC CAAGGAGGTG 
GACAAGGTCA ACCAGATCAT CAACAACTGC ATCGACGCCC TCAAGGCCGA GGCCACCTCC 
TTCCAGGGCG GCAAGCCGGG CGCCGTGTCC ACGGCCGAGC CGCTGGCCGG CAAGCGCGGC 
TTCCTGGCGC CCGCCTACCA GGACGCCTGC GGCCTGCAGC GGCACCACAG CCTGGAGGCC 
GCCCCCGGGG CCCCGCGGGC CAGCTCCTCG TCCAGCGGCT CCGCCCGCAG CCCGCGGCCC 
TACCGCGCCG AGCCCCCTGC GCTGCACGAG GCCAAGTACA TCGAGAAGGG CTCGCCCGCG 
GCGGAGGCCA TCCTCACGGT GACGCCCGCG GCAGCCGTGC TGCGGCCCGA GCCCGAGAAG 
GGCCGGCAGT ACGGGGAGCA CCGGCACTCG TACCCCGGGC CCCACCCTGT GGAGCCCCCC 
GCGCCGCCCG CGCCCCCCGA GAGCCTGGGC GGCCGCAAGG CCTCCATCCT GGAGCCGCTG 
ACGCGGCCGC GCCCCCGCGA CCTGGCCTAC GCGCCGCTCT CGCCGCAGTA CCGCCACCTG 
AGCTACGCCT CCAGCCCCGA GTACGCCTGC CGCGCCTCCC GCAGCCTCTG GGCCCGCTTC 
AGACTGAGCC GCCCGCGGCA CCGGGACGCC GGGGAGTGCG TGGCGGCCGG CCACGCGCTG 
CGCCGGAAGG TGCAGTTCGC CAAGGACGAG GACCTGCACG ACATCCTGGA CTACTGGAAG 
GGCGTGTCTG CGCAGCACAA GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193478.1
CDS	223..2649
Translation	MAGCGWEAPWVWVCVAAALLHAGGLVRGDCWLIEGDKGFVWLAICSQNQPPYEAIPQQINSTIVDLRLNENRIRSVQYAALSRFGNLTYLNLTKNEIGYIEDGAFSGQFNLQVLQLGYNRLRNLTEGVLRGLGKLEYLYLQANLIEAVAPGAFWECPNIVNVDLSMNRIQRLHSATFAGLARLSVCELYSNPFYCSCELLGFLRWLAAFANATQAHDRVQCESPPLYSGYFLLGQGRHGQRSILGKLQSVCTDGPYAAETRPAPGRPPPGRSPPPPPVPPAEPSEAPCAEDECFSGDGTTPLVALPTLAPQAEARPLIKVKQLTQNSATITVQLPSPFTRMYTLEHFNNSKSSTVSRLTRAQEEIRLTNLYALTNYTYCVVSSSSGLHHNHTCLTICLPQPPSPPGPVPSPSTATHYIMTVLGCLFGMVVVLGAVYYCLRRRRRREGKQKQAAAAAGSLKKTIIELKYGPELEAPGLAPLSQGPLLGPEAVTRIPYLPAAPGEVEQYQLAEGSGTPKGSKGGYMEVRAAEQAERREGEQGRPGPDSQSSVAEISTIAKEVDKVNQIINNCIDALKAEATSFQGGKPGAVSTAEPLAGKRGFLAPAYQDACGLQRHHSLEAAPGAPRASSSSSGSARSPRPYRAEPPALHEAKYIEKGSPAAEAILTVTPAAAVLRPEPEKGRQYGEHRHSYPGPHPVEPPAPPAPPESLGGRKASILEPLTRPRPRDLAYAPLSPQYRHLSYASSPEYACRASRSLWARFRLSRPRHRDAGECVAAGHALRRKVQFAKDEDLHDILDYWKGVSAQHKS

Target ORF information:

RefSeq Version	NM_001206549.2
Organism	Bos taurus(cattle)
Definition	Bos taurus extracellular leucine rich repeat and fibronectin type III domain containing 1 (ELFN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206549.2

ORF Insert Sequence:

ATGGCTGGGT GCGGGTGGGA GGCGCCGTGG GTGTGGGTGT GTGTGGCGGC CGCCCTGCTG 
CACGCGGGCG GGCTGGTGCG CGGAGACTGC TGGCTCATCG AGGGTGACAA GGGCTTCGTG 
TGGCTGGCCA TCTGCAGCCA GAACCAGCCG CCCTACGAGG CCATCCCGCA GCAGATCAAC 
AGTACCATCG TGGACCTGCG GCTCAACGAG AACCGCATCC GCAGCGTGCA GTACGCGGCG 
CTGAGCCGCT TCGGCAACCT CACGTACCTG AACCTCACCA AGAACGAGAT CGGCTACATC 
GAGGACGGCG CCTTCTCGGG CCAGTTCAAC CTGCAGGTAC TGCAGCTCGG CTACAACCGG 
CTGCGCAACC TGACGGAGGG CGTGCTGCGC GGCCTGGGCA AGCTCGAGTA CCTGTACCTG 
CAGGCCAACC TTATTGAGGC GGTGGCGCCC GGCGCCTTCT GGGAGTGCCC CAACATCGTC 
AACGTGGACC TGTCCATGAA CCGCATCCAG CGTCTGCACA GCGCCACCTT CGCGGGCCTG 
GCGCGGCTCT CGGTGTGTGA ACTCTACAGC AACCCCTTCT ACTGCTCCTG CGAGCTGCTG 
GGCTTCCTGC GCTGGCTGGC GGCCTTCGCC AACGCTACGC AGGCCCACGA CCGCGTGCAG 
TGCGAGTCCC CGCCGCTCTA CTCCGGCTAC TTCCTGCTGG GCCAGGGCCG CCACGGCCAG 
CGCAGCATCC TGGGCAAGCT GCAGTCTGTG TGCACTGACG GCCCGTATGC GGCGGAGACC 
CGCCCGGCGC CCGGCCGCCC CCCGCCCGGC CGCTCGCCGC CGCCGCCCCC CGTGCCGCCC 
GCGGAGCCCA GCGAGGCCCC CTGCGCTGAG GACGAGTGCT TCTCCGGCGA CGGCACCACG 
CCGCTGGTGG CGCTGCCCAC GCTGGCCCCG CAGGCCGAGG CCCGCCCGCT CATCAAGGTC 
AAGCAGCTGA CGCAGAACTC GGCCACCATC ACGGTGCAGC TGCCCAGCCC CTTCACCCGC 
ATGTACACGC TGGAGCACTT CAACAACAGC AAGTCGTCCA CCGTGTCCAG GCTGACCCGG 
GCCCAGGAGG AGATCCGCCT GACCAACCTG TACGCGCTCA CCAACTACAC CTATTGTGTG 
GTGTCCAGCA GCTCGGGCCT GCACCACAAC CACACCTGCC TCACCATCTG CCTGCCGCAG 
CCGCCTAGCC CGCCGGGCCC CGTGCCCAGC CCCTCCACGG CCACCCACTA CATCATGACC 
GTCCTGGGCT GCCTCTTCGG CATGGTGGTG GTGCTGGGCG CCGTCTACTA CTGCCTGCGG 
CGGCGGCGGC GGCGGGAGGG CAAGCAGAAG CAGGCGGCGG CAGCGGCCGG CAGCCTCAAG 
AAGACCATCA TCGAGCTCAA GTACGGGCCT GAGCTGGAGG CGCCAGGCCT GGCCCCGCTG 
TCCCAGGGCC CGCTGCTGGG CCCCGAGGCG GTGACCCGCA TCCCATACCT GCCCGCCGCC 
CCCGGGGAGG TGGAGCAGTA CCAGCTGGCG GAGGGCAGCG GGACACCCAA GGGCAGCAAG 
GGCGGCTACA TGGAGGTGCG TGCGGCAGAG CAGGCAGAAC GCAGGGAGGG GGAGCAGGGC 
CGGCCGGGCC CCGACAGCCA GAGCTCGGTG GCTGAGATCT CCACCATCGC CAAGGAGGTG 
GACAAGGTCA ACCAGATCAT CAACAACTGC ATCGACGCCC TCAAGGCCGA GGCCACCTCC 
TTCCAGGGCG GCAAGCCGGG CGCCGTGTCC ACGGCCGAGC CGCTGGCCGG CAAGCGCGGC 
TTCCTGGCGC CCGCCTACCA GGACGCCTGC GGCCTGCAGC GGCACCACAG CCTGGAGGCC 
GCCCCCGGGG CCCCGCGGGC CAGCTCCTCG TCCAGCGGCT CCGCCCGCAG CCCGCGGCCC 
TACCGCGCCG AGCCCCCTGC GCTGCACGAG GCCAAGTACA TCGAGAAGGG CTCGCCCGCG 
GCGGAGGCCA TCCTCACGGT GACGCCCGCG GCAGCCGTGC TGCGGCCCGA GCCCGAGAAG 
GGCCGGCAGT ACGGGGAGCA CCGGCACTCG TACCCCGGGC CCCACCCTGT GGAGCCCCCC 
GCGCCGCCCG CGCCCCCCGA GAGCCTGGGC GGCCGCAAGG CCTCCATCCT GGAGCCGCTG 
ACGCGGCCGC GCCCCCGCGA CCTGGCCTAC GCGCCGCTCT CGCCGCAGTA CCGCCACCTG 
AGCTACGCCT CCAGCCCCGA GTACGCCTGC CGCGCCTCCC GCAGCCTCTG GGCCCGCTTC 
AGACTGAGCC GCCCGCGGCA CCGGGACGCC GGGGAGTGCG TGGCGGCCGG CCACGCGCTG 
CGCCGGAAGG TGCAGTTCGC CAAGGACGAG GACCTGCACG ACATCCTGGA CTACTGGAAG 
GGCGTGTCTG CGCAGCACAA GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ELFN1 ( XM_005225229.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005225286.2 Bos taurus extracellular leucine rich repeat and fibronectin type III domain containing 1 (ELFN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa230430
Clone ID Related Accession (Same CDS sequence)	NM_001206549.2 , XM_005225229.3
Accession Version	XM_005225229.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein ELFN1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225229.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGT GCGGGTGGGA GGCGCCGTGG GTGTGGGTGT GTGTGGCGGC CGCCCTGCTG 
CACGCGGGCG GGCTGGTGCG CGGAGACTGC TGGCTCATCG AGGGTGACAA GGGCTTCGTG 
TGGCTGGCCA TCTGCAGCCA GAACCAGCCG CCCTACGAGG CCATCCCGCA GCAGATCAAC 
AGTACCATCG TGGACCTGCG GCTCAACGAG AACCGCATCC GCAGCGTGCA GTACGCGGCG 
CTGAGCCGCT TCGGCAACCT CACGTACCTG AACCTCACCA AGAACGAGAT CGGCTACATC 
GAGGACGGCG CCTTCTCGGG CCAGTTCAAC CTGCAGGTAC TGCAGCTCGG CTACAACCGG 
CTGCGCAACC TGACGGAGGG CGTGCTGCGC GGCCTGGGCA AGCTCGAGTA CCTGTACCTG 
CAGGCCAACC TTATTGAGGC GGTGGCGCCC GGCGCCTTCT GGGAGTGCCC CAACATCGTC 
AACGTGGACC TGTCCATGAA CCGCATCCAG CGTCTGCACA GCGCCACCTT CGCGGGCCTG 
GCGCGGCTCT CGGTGTGTGA ACTCTACAGC AACCCCTTCT ACTGCTCCTG CGAGCTGCTG 
GGCTTCCTGC GCTGGCTGGC GGCCTTCGCC AACGCTACGC AGGCCCACGA CCGCGTGCAG 
TGCGAGTCCC CGCCGCTCTA CTCCGGCTAC TTCCTGCTGG GCCAGGGCCG CCACGGCCAG 
CGCAGCATCC TGGGCAAGCT GCAGTCTGTG TGCACTGACG GCCCGTATGC GGCGGAGACC 
CGCCCGGCGC CCGGCCGCCC CCCGCCCGGC CGCTCGCCGC CGCCGCCCCC CGTGCCGCCC 
GCGGAGCCCA GCGAGGCCCC CTGCGCTGAG GACGAGTGCT TCTCCGGCGA CGGCACCACG 
CCGCTGGTGG CGCTGCCCAC GCTGGCCCCG CAGGCCGAGG CCCGCCCGCT CATCAAGGTC 
AAGCAGCTGA CGCAGAACTC GGCCACCATC ACGGTGCAGC TGCCCAGCCC CTTCACCCGC 
ATGTACACGC TGGAGCACTT CAACAACAGC AAGTCGTCCA CCGTGTCCAG GCTGACCCGG 
GCCCAGGAGG AGATCCGCCT GACCAACCTG TACGCGCTCA CCAACTACAC CTATTGTGTG 
GTGTCCAGCA GCTCGGGCCT GCACCACAAC CACACCTGCC TCACCATCTG CCTGCCGCAG 
CCGCCTAGCC CGCCGGGCCC CGTGCCCAGC CCCTCCACGG CCACCCACTA CATCATGACC 
GTCCTGGGCT GCCTCTTCGG CATGGTGGTG GTGCTGGGCG CCGTCTACTA CTGCCTGCGG 
CGGCGGCGGC GGCGGGAGGG CAAGCAGAAG CAGGCGGCGG CAGCGGCCGG CAGCCTCAAG 
AAGACCATCA TCGAGCTCAA GTACGGGCCT GAGCTGGAGG CGCCAGGCCT GGCCCCGCTG 
TCCCAGGGCC CGCTGCTGGG CCCCGAGGCG GTGACCCGCA TCCCATACCT GCCCGCCGCC 
CCCGGGGAGG TGGAGCAGTA CCAGCTGGCG GAGGGCAGCG GGACACCCAA GGGCAGCAAG 
GGCGGCTACA TGGAGGTGCG TGCGGCAGAG CAGGCAGAAC GCAGGGAGGG GGAGCAGGGC 
CGGCCGGGCC CCGACAGCCA GAGCTCGGTG GCTGAGATCT CCACCATCGC CAAGGAGGTG 
GACAAGGTCA ACCAGATCAT CAACAACTGC ATCGACGCCC TCAAGGCCGA GGCCACCTCC 
TTCCAGGGCG GCAAGCCGGG CGCCGTGTCC ACGGCCGAGC CGCTGGCCGG CAAGCGCGGC 
TTCCTGGCGC CCGCCTACCA GGACGCCTGC GGCCTGCAGC GGCACCACAG CCTGGAGGCC 
GCCCCCGGGG CCCCGCGGGC CAGCTCCTCG TCCAGCGGCT CCGCCCGCAG CCCGCGGCCC 
TACCGCGCCG AGCCCCCTGC GCTGCACGAG GCCAAGTACA TCGAGAAGGG CTCGCCCGCG 
GCGGAGGCCA TCCTCACGGT GACGCCCGCG GCAGCCGTGC TGCGGCCCGA GCCCGAGAAG 
GGCCGGCAGT ACGGGGAGCA CCGGCACTCG TACCCCGGGC CCCACCCTGT GGAGCCCCCC 
GCGCCGCCCG CGCCCCCCGA GAGCCTGGGC GGCCGCAAGG CCTCCATCCT GGAGCCGCTG 
ACGCGGCCGC GCCCCCGCGA CCTGGCCTAC GCGCCGCTCT CGCCGCAGTA CCGCCACCTG 
AGCTACGCCT CCAGCCCCGA GTACGCCTGC CGCGCCTCCC GCAGCCTCTG GGCCCGCTTC 
AGACTGAGCC GCCCGCGGCA CCGGGACGCC GGGGAGTGCG TGGCGGCCGG CCACGCGCTG 
CGCCGGAAGG TGCAGTTCGC CAAGGACGAG GACCTGCACG ACATCCTGGA CTACTGGAAG 
GGCGTGTCTG CGCAGCACAA GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005225286.2
CDS	673..3099
Translation	MAGCGWEAPWVWVCVAAALLHAGGLVRGDCWLIEGDKGFVWLAICSQNQPPYEAIPQQINSTIVDLRLNENRIRSVQYAALSRFGNLTYLNLTKNEIGYIEDGAFSGQFNLQVLQLGYNRLRNLTEGVLRGLGKLEYLYLQANLIEAVAPGAFWECPNIVNVDLSMNRIQRLHSATFAGLARLSVCELYSNPFYCSCELLGFLRWLAAFANATQAHDRVQCESPPLYSGYFLLGQGRHGQRSILGKLQSVCTDGPYAAETRPAPGRPPPGRSPPPPPVPPAEPSEAPCAEDECFSGDGTTPLVALPTLAPQAEARPLIKVKQLTQNSATITVQLPSPFTRMYTLEHFNNSKSSTVSRLTRAQEEIRLTNLYALTNYTYCVVSSSSGLHHNHTCLTICLPQPPSPPGPVPSPSTATHYIMTVLGCLFGMVVVLGAVYYCLRRRRRREGKQKQAAAAAGSLKKTIIELKYGPELEAPGLAPLSQGPLLGPEAVTRIPYLPAAPGEVEQYQLAEGSGTPKGSKGGYMEVRAAEQAERREGEQGRPGPDSQSSVAEISTIAKEVDKVNQIINNCIDALKAEATSFQGGKPGAVSTAEPLAGKRGFLAPAYQDACGLQRHHSLEAAPGAPRASSSSSGSARSPRPYRAEPPALHEAKYIEKGSPAAEAILTVTPAAAVLRPEPEKGRQYGEHRHSYPGPHPVEPPAPPAPPESLGGRKASILEPLTRPRPRDLAYAPLSPQYRHLSYASSPEYACRASRSLWARFRLSRPRHRDAGECVAAGHALRRKVQFAKDEDLHDILDYWKGVSAQHKS

Target ORF information:

RefSeq Version	XM_005225229.3
Organism	Bos taurus(cattle)
Definition	Bos taurus extracellular leucine rich repeat and fibronectin type III domain containing 1 (ELFN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005225229.3

ORF Insert Sequence:

ATGGCTGGGT GCGGGTGGGA GGCGCCGTGG GTGTGGGTGT GTGTGGCGGC CGCCCTGCTG 
CACGCGGGCG GGCTGGTGCG CGGAGACTGC TGGCTCATCG AGGGTGACAA GGGCTTCGTG 
TGGCTGGCCA TCTGCAGCCA GAACCAGCCG CCCTACGAGG CCATCCCGCA GCAGATCAAC 
AGTACCATCG TGGACCTGCG GCTCAACGAG AACCGCATCC GCAGCGTGCA GTACGCGGCG 
CTGAGCCGCT TCGGCAACCT CACGTACCTG AACCTCACCA AGAACGAGAT CGGCTACATC 
GAGGACGGCG CCTTCTCGGG CCAGTTCAAC CTGCAGGTAC TGCAGCTCGG CTACAACCGG 
CTGCGCAACC TGACGGAGGG CGTGCTGCGC GGCCTGGGCA AGCTCGAGTA CCTGTACCTG 
CAGGCCAACC TTATTGAGGC GGTGGCGCCC GGCGCCTTCT GGGAGTGCCC CAACATCGTC 
AACGTGGACC TGTCCATGAA CCGCATCCAG CGTCTGCACA GCGCCACCTT CGCGGGCCTG 
GCGCGGCTCT CGGTGTGTGA ACTCTACAGC AACCCCTTCT ACTGCTCCTG CGAGCTGCTG 
GGCTTCCTGC GCTGGCTGGC GGCCTTCGCC AACGCTACGC AGGCCCACGA CCGCGTGCAG 
TGCGAGTCCC CGCCGCTCTA CTCCGGCTAC TTCCTGCTGG GCCAGGGCCG CCACGGCCAG 
CGCAGCATCC TGGGCAAGCT GCAGTCTGTG TGCACTGACG GCCCGTATGC GGCGGAGACC 
CGCCCGGCGC CCGGCCGCCC CCCGCCCGGC CGCTCGCCGC CGCCGCCCCC CGTGCCGCCC 
GCGGAGCCCA GCGAGGCCCC CTGCGCTGAG GACGAGTGCT TCTCCGGCGA CGGCACCACG 
CCGCTGGTGG CGCTGCCCAC GCTGGCCCCG CAGGCCGAGG CCCGCCCGCT CATCAAGGTC 
AAGCAGCTGA CGCAGAACTC GGCCACCATC ACGGTGCAGC TGCCCAGCCC CTTCACCCGC 
ATGTACACGC TGGAGCACTT CAACAACAGC AAGTCGTCCA CCGTGTCCAG GCTGACCCGG 
GCCCAGGAGG AGATCCGCCT GACCAACCTG TACGCGCTCA CCAACTACAC CTATTGTGTG 
GTGTCCAGCA GCTCGGGCCT GCACCACAAC CACACCTGCC TCACCATCTG CCTGCCGCAG 
CCGCCTAGCC CGCCGGGCCC CGTGCCCAGC CCCTCCACGG CCACCCACTA CATCATGACC 
GTCCTGGGCT GCCTCTTCGG CATGGTGGTG GTGCTGGGCG CCGTCTACTA CTGCCTGCGG 
CGGCGGCGGC GGCGGGAGGG CAAGCAGAAG CAGGCGGCGG CAGCGGCCGG CAGCCTCAAG 
AAGACCATCA TCGAGCTCAA GTACGGGCCT GAGCTGGAGG CGCCAGGCCT GGCCCCGCTG 
TCCCAGGGCC CGCTGCTGGG CCCCGAGGCG GTGACCCGCA TCCCATACCT GCCCGCCGCC 
CCCGGGGAGG TGGAGCAGTA CCAGCTGGCG GAGGGCAGCG GGACACCCAA GGGCAGCAAG 
GGCGGCTACA TGGAGGTGCG TGCGGCAGAG CAGGCAGAAC GCAGGGAGGG GGAGCAGGGC 
CGGCCGGGCC CCGACAGCCA GAGCTCGGTG GCTGAGATCT CCACCATCGC CAAGGAGGTG 
GACAAGGTCA ACCAGATCAT CAACAACTGC ATCGACGCCC TCAAGGCCGA GGCCACCTCC 
TTCCAGGGCG GCAAGCCGGG CGCCGTGTCC ACGGCCGAGC CGCTGGCCGG CAAGCGCGGC 
TTCCTGGCGC CCGCCTACCA GGACGCCTGC GGCCTGCAGC GGCACCACAG CCTGGAGGCC 
GCCCCCGGGG CCCCGCGGGC CAGCTCCTCG TCCAGCGGCT CCGCCCGCAG CCCGCGGCCC 
TACCGCGCCG AGCCCCCTGC GCTGCACGAG GCCAAGTACA TCGAGAAGGG CTCGCCCGCG 
GCGGAGGCCA TCCTCACGGT GACGCCCGCG GCAGCCGTGC TGCGGCCCGA GCCCGAGAAG 
GGCCGGCAGT ACGGGGAGCA CCGGCACTCG TACCCCGGGC CCCACCCTGT GGAGCCCCCC 
GCGCCGCCCG CGCCCCCCGA GAGCCTGGGC GGCCGCAAGG CCTCCATCCT GGAGCCGCTG 
ACGCGGCCGC GCCCCCGCGA CCTGGCCTAC GCGCCGCTCT CGCCGCAGTA CCGCCACCTG 
AGCTACGCCT CCAGCCCCGA GTACGCCTGC CGCGCCTCCC GCAGCCTCTG GGCCCGCTTC 
AGACTGAGCC GCCCGCGGCA CCGGGACGCC GGGGAGTGCG TGGCGGCCGG CCACGCGCTG 
CGCCGGAAGG TGCAGTTCGC CAAGGACGAG GACCTGCACG ACATCCTGGA CTACTGGAAG 
GGCGTGTCTG CGCAGCACAA GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ELFN1 ( NM_001206549.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193478.1 Bos taurus extracellular leucine-rich repeat and fibronectin type III domain containing 1 (ELFN1), mRNA.

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CloneID	OBa01963
Clone ID Related Accession (Same CDS sequence)	NM_001206549.1
Accession Version	NM_001206549.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-31
Organism	Bos taurus(cattle)
Product	protein ELFN1 precursor
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AAFC03041043.1. On or before May 4, 2011 this sequence version replaced XM_871098.4, XM_002698233.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGGCTGGGT GCGGGTGGGA GGCGCCGTGG GTGTGGGTGT GTGTGGCGGC CGCCCTGCTG 
CACGCGGGCG GGCTGGTGCG CGGAGACTGC TGGCTCATCG AGGGTGACAA GGGCTTCGTG 
TGGCTGGCCA TCTGCAGCCA GAACCAGCCG CCCTACGAGG CCATCCCGCA GCAGATCAAC 
AGTACCATCG TGGACCTGCG GCTCAACGAG AACCGCATCC GCAGCGTGCA GTACGCGGCG 
CTGAGCCGCT TCGGCAACCT CACGTACCTG AACCTCACCA AGAACGAGAT CGGCTACATC 
GAGGACGGCG CCTTCTCGGG CCAGTTCAAC CTGCAGGTAC TGCAGCTCGG CTACAACCGG 
CTGCGCAACC TGACGGAGGG CGTGCTGCGC GGCCTGGGCA AGCTCGAGTA CCTGTACCTG 
CAGGCCAACC TTATTGAGGC GGTGGCGCCC GGCGCCTTCT GGGAGTGCCC CAACATCGTC 
AACGTGGACC TGTCCATGAA CCGCATCCAG CGTCTGCACA GCGCCACCTT CGCGGGCCTG 
GCGCGGCTCT CGGTGTGTGA ACTCTACAGC AACCCCTTCT ACTGCTCCTG CGAGCTGCTG 
GGCTTCCTGC GCTGGCTGGC GGCCTTCGCC AACGCTACGC AGGCCCACGA CCGCGTGCAG 
TGCGAGTCCC CGCCGCTCTA CTCCGGCTAC TTCCTGCTGG GCCAGGGCCG CCACGGCCAG 
CGCAGCATCC TGGGCAAGCT GCAGTCTGTG TGCACTGACG GCCCGTATGC GGCGGAGACC 
CGCCCGGCGC CCGGCCGCCC CCCGCCCGGC CGCTCGCCGC CGCCGCCCCC CGTGCCGCCC 
GCGGAGCCCA GCGAGGCCCC CTGCGCTGAG GACGAGTGCT TCTCCGGCGA CGGCACCACG 
CCGCTGGTGG CGCTGCCCAC GCTGGCCCCG CAGGCCGAGG CCCGCCCGCT CATCAAGGTC 
AAGCAGCTGA CGCAGAACTC GGCCACCATC ACGGTGCAGC TGCCCAGCCC CTTCACCCGC 
ATGTACACGC TGGAGCACTT CAACAACAGC AAGTCGTCCA CCGTGTCCAG GCTGACCCGG 
GCCCAGGAGG AGATCCGCCT GACCAACCTG TACGCGCTCA CCAACTACAC CTATTGTGTG 
GTGTCCAGCA GCTCGGGCCT GCACCACAAC CACACCTGCC TCACCATCTG CCTGCCGCAG 
CCGCCTAGCC CGCCGGGCCC CGTGCCCAGC CCCTCCACGG CCACCCACTA CATCATGACC 
GTCCTGGGCT GCCTCTTCGG CATGGTGGTG GTGCTGGGCG CCGTCTACTA CTGCCTGCGG 
CGGCGGCGGC GGCGGGAGGG CAAGCAGAAG CAGGCGGCGG CAGCGGCCGG CAGCCTCAAG 
AAGACCATCA TCGAGCTCAA GTACGGGCCT GAGCTGGAGG CGCCAGGCCT GGCCCCGCTG 
TCCCAGGGCC CGCTGCTGGG CCCCGAGGCG GTGACCCGCA TCCCATACCT GCCCGCCGCC 
CCCGGGGAGG TGGAGCAGTA CCAGCTGGCG GAGGGCAGCG GGACACCCAA GGGCAGCAAG 
GGCGGCTACA TGGAGGTGCG TGCGGCAGAG CAGGCAGAAC GCAGGGAGGG GGAGCAGGGC 
CGGCCGGGCC CCGACAGCCA GAGCTCGGTG GCTGAGATCT CCACCATCGC CAAGGAGGTG 
GACAAGGTCA ACCAGATCAT CAACAACTGC ATCGACGCCC TCAAGGCCGA GGCCACCTCC 
TTCCAGGGCG GCAAGCCGGG CGCCGTGTCC ACGGCCGAGC CGCTGGCCGG CAAGCGCGGC 
TTCCTGGCGC CCGCCTACCA GGACGCCTGC GGCCTGCAGC GGCACCACAG CCTGGAGGCC 
GCCCCCGGGG CCCCGCGGGC CAGCTCCTCG TCCAGCGGCT CCGCCCGCAG CCCGCGGCCC 
TACCGCGCCG AGCCTCCTGC GCTGCACGAG GCCAAGTACA TCGAGAAGGG CTCGCCCGCG 
GCGGAGGCCA TCCTCACGGT GACGCCCGCG GCAGCCGTGC TGCGGCCCGA GCCCGAGAAG 
GGCCGGCAGT ACGGGGAGCA CCGGCACTCG TACCCCGGGC CCCACCCTGT GGAGCCCCCC 
GCGCCGCCCG CGCCCCCCGA GAGCCTGGGC GGCCGCAAGG CCTCCATCCT GGAGCCGCTG 
ACGCGGCCGC GCCCCCGCGA CCTGGCCTAC GCGCCGCTCT CGCCGCAGTA CCGCCACCTG 
AGCTACGCCT CCAGCCCCGA GTACGCCTGC CGCGCCTCCC GCAGCCTCTG GGCCCGCTTC 
AGACTGAGCC GCCCGCGGCA CCGGGACGCC GGGGAGTGCG TGGCGGCCGG CCACGCGCTG 
CGCCGGAAGG TGCAGTTCGC CAAGGACGAG GACCTGCACG ACATCCTGGA CTACTGGAAG 
GGCGTGTCTG CGCAGCACAA GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193478.1
CDS	223..2649
Translation	MAGCGWEAPWVWVCVAAALLHAGGLVRGDCWLIEGDKGFVWLAICSQNQPPYEAIPQQINSTIVDLRLNENRIRSVQYAALSRFGNLTYLNLTKNEIGYIEDGAFSGQFNLQVLQLGYNRLRNLTEGVLRGLGKLEYLYLQANLIEAVAPGAFWECPNIVNVDLSMNRIQRLHSATFAGLARLSVCELYSNPFYCSCELLGFLRWLAAFANATQAHDRVQCESPPLYSGYFLLGQGRHGQRSILGKLQSVCTDGPYAAETRPAPGRPPPGRSPPPPPVPPAEPSEAPCAEDECFSGDGTTPLVALPTLAPQAEARPLIKVKQLTQNSATITVQLPSPFTRMYTLEHFNNSKSSTVSRLTRAQEEIRLTNLYALTNYTYCVVSSSSGLHHNHTCLTICLPQPPSPPGPVPSPSTATHYIMTVLGCLFGMVVVLGAVYYCLRRRRRREGKQKQAAAAAGSLKKTIIELKYGPELEAPGLAPLSQGPLLGPEAVTRIPYLPAAPGEVEQYQLAEGSGTPKGSKGGYMEVRAAEQAERREGEQGRPGPDSQSSVAEISTIAKEVDKVNQIINNCIDALKAEATSFQGGKPGAVSTAEPLAGKRGFLAPAYQDACGLQRHHSLEAAPGAPRASSSSSGSARSPRPYRAEPPALHEAKYIEKGSPAAEAILTVTPAAAVLRPEPEKGRQYGEHRHSYPGPHPVEPPAPPAPPESLGGRKASILEPLTRPRPRDLAYAPLSPQYRHLSYASSPEYACRASRSLWARFRLSRPRHRDAGECVAAGHALRRKVQFAKDEDLHDILDYWKGVSAQHKS

Target ORF information:

RefSeq Version	NM_001206549.1
Organism	Bos taurus(cattle)
Definition	Bos taurus extracellular leucine-rich repeat and fibronectin type III domain containing 1 (ELFN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206549.1

ORF Insert Sequence:

ATGGCTGGGT GCGGGTGGGA GGCGCCGTGG GTGTGGGTGT GTGTGGCGGC CGCCCTGCTG 
CACGCGGGCG GGCTGGTGCG CGGAGACTGC TGGCTCATCG AGGGTGACAA GGGCTTCGTG 
TGGCTGGCCA TCTGCAGCCA GAACCAGCCG CCCTACGAGG CCATCCCGCA GCAGATCAAC 
AGTACCATCG TGGACCTGCG GCTCAACGAG AACCGCATCC GCAGCGTGCA GTACGCGGCG 
CTGAGCCGCT TCGGCAACCT CACGTACCTG AACCTCACCA AGAACGAGAT CGGCTACATC 
GAGGACGGCG CCTTCTCGGG CCAGTTCAAC CTGCAGGTAC TGCAGCTCGG CTACAACCGG 
CTGCGCAACC TGACGGAGGG CGTGCTGCGC GGCCTGGGCA AGCTCGAGTA CCTGTACCTG 
CAGGCCAACC TTATTGAGGC GGTGGCGCCC GGCGCCTTCT GGGAGTGCCC CAACATCGTC 
AACGTGGACC TGTCCATGAA CCGCATCCAG CGTCTGCACA GCGCCACCTT CGCGGGCCTG 
GCGCGGCTCT CGGTGTGTGA ACTCTACAGC AACCCCTTCT ACTGCTCCTG CGAGCTGCTG 
GGCTTCCTGC GCTGGCTGGC GGCCTTCGCC AACGCTACGC AGGCCCACGA CCGCGTGCAG 
TGCGAGTCCC CGCCGCTCTA CTCCGGCTAC TTCCTGCTGG GCCAGGGCCG CCACGGCCAG 
CGCAGCATCC TGGGCAAGCT GCAGTCTGTG TGCACTGACG GCCCGTATGC GGCGGAGACC 
CGCCCGGCGC CCGGCCGCCC CCCGCCCGGC CGCTCGCCGC CGCCGCCCCC CGTGCCGCCC 
GCGGAGCCCA GCGAGGCCCC CTGCGCTGAG GACGAGTGCT TCTCCGGCGA CGGCACCACG 
CCGCTGGTGG CGCTGCCCAC GCTGGCCCCG CAGGCCGAGG CCCGCCCGCT CATCAAGGTC 
AAGCAGCTGA CGCAGAACTC GGCCACCATC ACGGTGCAGC TGCCCAGCCC CTTCACCCGC 
ATGTACACGC TGGAGCACTT CAACAACAGC AAGTCGTCCA CCGTGTCCAG GCTGACCCGG 
GCCCAGGAGG AGATCCGCCT GACCAACCTG TACGCGCTCA CCAACTACAC CTATTGTGTG 
GTGTCCAGCA GCTCGGGCCT GCACCACAAC CACACCTGCC TCACCATCTG CCTGCCGCAG 
CCGCCTAGCC CGCCGGGCCC CGTGCCCAGC CCCTCCACGG CCACCCACTA CATCATGACC 
GTCCTGGGCT GCCTCTTCGG CATGGTGGTG GTGCTGGGCG CCGTCTACTA CTGCCTGCGG 
CGGCGGCGGC GGCGGGAGGG CAAGCAGAAG CAGGCGGCGG CAGCGGCCGG CAGCCTCAAG 
AAGACCATCA TCGAGCTCAA GTACGGGCCT GAGCTGGAGG CGCCAGGCCT GGCCCCGCTG 
TCCCAGGGCC CGCTGCTGGG CCCCGAGGCG GTGACCCGCA TCCCATACCT GCCCGCCGCC 
CCCGGGGAGG TGGAGCAGTA CCAGCTGGCG GAGGGCAGCG GGACACCCAA GGGCAGCAAG 
GGCGGCTACA TGGAGGTGCG TGCGGCAGAG CAGGCAGAAC GCAGGGAGGG GGAGCAGGGC 
CGGCCGGGCC CCGACAGCCA GAGCTCGGTG GCTGAGATCT CCACCATCGC CAAGGAGGTG 
GACAAGGTCA ACCAGATCAT CAACAACTGC ATCGACGCCC TCAAGGCCGA GGCCACCTCC 
TTCCAGGGCG GCAAGCCGGG CGCCGTGTCC ACGGCCGAGC CGCTGGCCGG CAAGCGCGGC 
TTCCTGGCGC CCGCCTACCA GGACGCCTGC GGCCTGCAGC GGCACCACAG CCTGGAGGCC 
GCCCCCGGGG CCCCGCGGGC CAGCTCCTCG TCCAGCGGCT CCGCCCGCAG CCCGCGGCCC 
TACCGCGCCG AGCCTCCTGC GCTGCACGAG GCCAAGTACA TCGAGAAGGG CTCGCCCGCG 
GCGGAGGCCA TCCTCACGGT GACGCCCGCG GCAGCCGTGC TGCGGCCCGA GCCCGAGAAG 
GGCCGGCAGT ACGGGGAGCA CCGGCACTCG TACCCCGGGC CCCACCCTGT GGAGCCCCCC 
GCGCCGCCCG CGCCCCCCGA GAGCCTGGGC GGCCGCAAGG CCTCCATCCT GGAGCCGCTG 
ACGCGGCCGC GCCCCCGCGA CCTGGCCTAC GCGCCGCTCT CGCCGCAGTA CCGCCACCTG 
AGCTACGCCT CCAGCCCCGA GTACGCCTGC CGCGCCTCCC GCAGCCTCTG GGCCCGCTTC 
AGACTGAGCC GCCCGCGGCA CCGGGACGCC GGGGAGTGCG TGGCGGCCGG CCACGCGCTG 
CGCCGGAAGG TGCAGTTCGC CAAGGACGAG GACCTGCACG ACATCCTGGA CTACTGGAAG 
GGCGTGTCTG CGCAGCACAA GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ELFN1 ( XM_005225229.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005225286.1 Bos taurus extracellular leucine-rich repeat and fibronectin type III domain containing 1 (ELFN1), transcript variant X1, mRNA.

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CloneID	OBa33325
Clone ID Related Accession (Same CDS sequence)	XM_005225229.2
Accession Version	XM_005225229.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein ELFN1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005225229.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGT GCGGGTGGGA GGCGCCGTGG GTGTGGGTGT GTGTGGCGGC CGCCCTGCTG 
CACGCGGGCG GGCTGGTGCG CGGTGACTGC TGGCTCATCG AGGGTGACAA GGGCTTCGTG 
TGGCTGGCCA TCTGCAGCCA GAACCAGCCG CCCTACGAGG CCATCCCGCA GCAGATCAAC 
AGTACCATCG TGGACCTGCG GCTCAACGAG AACCGCATCC GCAGCGTGCA GTACGCGGCG 
CTGAGCCGCT TCGGCAACCT CACGTACCTG AACCTCACCA AGAACGAGAT CGGCTACATC 
GAGGACGGCG CCTTCTCGGG CCAGTTCAAC CTGCAGGTAC TGCAGCTCGG CTACAACCGG 
CTGCGCAACC TGACGGAGGG CGTGCTGCGC GGCCTGGGCA AGCTCGAGTA CCTGTACCTG 
CAGGCCAACC TTATTGAGGC GGTGGCGCCC GGCGCCTTCT GGGAGTGCCC CAACATCGTC 
AACGTGGACC TTTCCATGAA CCGCATCCAG CGTCTGCACA GCGCCACCTT CGCGGGCCTG 
GCGCGGCTCT CGGTGTGTGA ACTCTACAGC AACCCCTTCT ACTGCTCCTG CGAGCTGCTG 
GGCTTCCTGC GCTGGCTGGC GGCCTTCGCC AACGCTACGC AGGCCCACGA CCGCGTGCAG 
TGCGAGTCCC CGCCGCTCTA CTCCGGCTAC TTCCTGCTGG GCCAGGGCCG CCACGGCCAG 
CGCAGCATCC TGGGCAAGCT GCAGTCTGTG TGCACTGACG GCCCGTATGC GGCGGAGACC 
CGCCCGGCGC CCGGCCGCCC CCCGCCCGGC CGCTCGCCGC CGCCGCCCCC CGAGCCCAGC 
GAGGCCCCCT GCGCTGAGGA CGAGTGCTTC TCCGGCGACG GCACCACGCC GCTGCCCACG 
CTGGCCCCGC AGGCCGAGGC CCGCCCGCTC ATCAAGGTCA AGCAGCTGAC GCAGAACTCG 
GCCACCATCA CGGTGCAGCT GCCCAGCCCC TTCACCCGCA TGTACACGCT GGAGCACTTC 
AACAACAGCA AGTCGTCCAC CGTGTCCAGG CTGACCCGGG CCCAGGAGGA GATCCGCCTG 
ACCAACCTGT ACGCGCTCAC CAACTACACC TATTGTGTGG TGTCCAGCAG CTCGGGCCTG 
CACCACAACC ACACCTGCCT CACCATCTGC CTGCCGCAGC CGCCTAGCCC GCCGGGCCCC 
GTGCCCAGCC CCTCCACGGC CACCCACTAC ATCATGACCG TCCTGGGCTG CCTCTTCGGC 
ATGGTGGTGG TGCTGGGCGC CGTCTACTAC TGCCTGCGGC GGCGGCGGCG GCGGGAGGGC 
AAGCAGAAGC AGGCGGCGGC AGCGGCCGGC AGCCTCAAGA AGACCATCAT CGAGCTCAAG 
TACGGGCCTG AGCTGGAGGC GCCAGGCCTG GCCCCGCTGT CCCAGGGCCC GCTGCTGGGC 
CCCGAGGCGG TGACCCGCAT CCCATACCTG CCCGCCGCCC CCGGGGAGGT GGAGCAGTAC 
CAGCTGGCGG AGGGCAGCGG GACACCCAAG GGCAGCAAGG GCGGCTACAT GGAGGTGCGT 
GCGGCAGAGC AGGCAGAACG CAGGGAGGGG GAGCAGGGCC GGCCGGGCCC CGACAGCCAG 
AGCTCGGTGG CTGAGATCTC CACCATCGCC AAGGAGGTGG ACAAGGTCAA CCAGATCATC 
AACAACTGCA TCGACGCCCT CAAGGCCGAG GCCACCTCCT TCCAGGGCGG CAAGCCGGGC 
GCCGTGTCCA CGGCCGAGCC GCTGGCCGGC AAGCGCGGCT TCCTGGCGCC CGCCTACCAG 
GACGCCTGCG GCCTGCAGCG GCACCACAGC CTGGAGGCCG CCCCCGGGGC CCCGCGGGCC 
AGCTCCTCGT CCAGCGGCTC CGCCCGCAGC CCGCGGCCCT ACCGCGCCGA GCCCCCTGCG 
CTGCACGAGG CCAAGTACAT CGAGAAGGGC TCGCCCGCGG CGGAGGCCAT CCTCACGGTG 
ACGCCCGCGG CAGCCGTGCT GCGGCCCGAG CCCGAGAAGG GCCGGCAGTA CGGGGAGCAC 
CGGCACTCGT ACCCCGGGCC CCACCCTGTG GAGCCCCCCG CGCCGCCCGC GCCCCCCGAG 
AGCCTGGGCG GCCGCAAGGC CTCCATCCTG GAGCCGCTGA CGCGGCCGCG CCCCCGCGAC 
CTGGCCTACG CGCCGCTCTC GCCGCAGTAC CGCCACCTGA GCTACGCCTC CAGCCCCGAG 
TACGCCTGCC GCGCCTCCCG CAGCCTCTGG GCCCGCTTCA GACTGAGCCG CCCGCGGCAC 
CGGGACGCCG GGGAGTGCGT GGCGGCCGGC CACGCGCTGC GCCGGAAGGT GCAGTTCGCC 
AAGGACGAGG ACCTGCACGA CATCCTGGAC TACTGGAAGG GCGTGTCTGC GCAGCACAAG 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005225286.1
CDS	468..2873
Translation	MAGCGWEAPWVWVCVAAALLHAGGLVRGDCWLIEGDKGFVWLAICSQNQPPYEAIPQQINSTIVDLRLNENRIRSVQYAALSRFGNLTYLNLTKNEIGYIEDGAFSGQFNLQVLQLGYNRLRNLTEGVLRGLGKLEYLYLQANLIEAVAPGAFWECPNIVNVDLSMNRIQRLHSATFAGLARLSVCELYSNPFYCSCELLGFLRWLAAFANATQAHDRVQCESPPLYSGYFLLGQGRHGQRSILGKLQSVCTDGPYAAETRPAPGRPPPGRSPPPPPEPSEAPCAEDECFSGDGTTPLPTLAPQAEARPLIKVKQLTQNSATITVQLPSPFTRMYTLEHFNNSKSSTVSRLTRAQEEIRLTNLYALTNYTYCVVSSSSGLHHNHTCLTICLPQPPSPPGPVPSPSTATHYIMTVLGCLFGMVVVLGAVYYCLRRRRRREGKQKQAAAAAGSLKKTIIELKYGPELEAPGLAPLSQGPLLGPEAVTRIPYLPAAPGEVEQYQLAEGSGTPKGSKGGYMEVRAAEQAERREGEQGRPGPDSQSSVAEISTIAKEVDKVNQIINNCIDALKAEATSFQGGKPGAVSTAEPLAGKRGFLAPAYQDACGLQRHHSLEAAPGAPRASSSSSGSARSPRPYRAEPPALHEAKYIEKGSPAAEAILTVTPAAAVLRPEPEKGRQYGEHRHSYPGPHPVEPPAPPAPPESLGGRKASILEPLTRPRPRDLAYAPLSPQYRHLSYASSPEYACRASRSLWARFRLSRPRHRDAGECVAAGHALRRKVQFAKDEDLHDILDYWKGVSAQHKS

Target ORF information:

RefSeq Version	XM_005225229.2
Organism	Bos taurus(cattle)
Definition	Bos taurus extracellular leucine-rich repeat and fibronectin type III domain containing 1 (ELFN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005225229.2

ORF Insert Sequence:

ATGGCTGGGT GCGGGTGGGA GGCGCCGTGG GTGTGGGTGT GTGTGGCGGC CGCCCTGCTG 
CACGCGGGCG GGCTGGTGCG CGGTGACTGC TGGCTCATCG AGGGTGACAA GGGCTTCGTG 
TGGCTGGCCA TCTGCAGCCA GAACCAGCCG CCCTACGAGG CCATCCCGCA GCAGATCAAC 
AGTACCATCG TGGACCTGCG GCTCAACGAG AACCGCATCC GCAGCGTGCA GTACGCGGCG 
CTGAGCCGCT TCGGCAACCT CACGTACCTG AACCTCACCA AGAACGAGAT CGGCTACATC 
GAGGACGGCG CCTTCTCGGG CCAGTTCAAC CTGCAGGTAC TGCAGCTCGG CTACAACCGG 
CTGCGCAACC TGACGGAGGG CGTGCTGCGC GGCCTGGGCA AGCTCGAGTA CCTGTACCTG 
CAGGCCAACC TTATTGAGGC GGTGGCGCCC GGCGCCTTCT GGGAGTGCCC CAACATCGTC 
AACGTGGACC TTTCCATGAA CCGCATCCAG CGTCTGCACA GCGCCACCTT CGCGGGCCTG 
GCGCGGCTCT CGGTGTGTGA ACTCTACAGC AACCCCTTCT ACTGCTCCTG CGAGCTGCTG 
GGCTTCCTGC GCTGGCTGGC GGCCTTCGCC AACGCTACGC AGGCCCACGA CCGCGTGCAG 
TGCGAGTCCC CGCCGCTCTA CTCCGGCTAC TTCCTGCTGG GCCAGGGCCG CCACGGCCAG 
CGCAGCATCC TGGGCAAGCT GCAGTCTGTG TGCACTGACG GCCCGTATGC GGCGGAGACC 
CGCCCGGCGC CCGGCCGCCC CCCGCCCGGC CGCTCGCCGC CGCCGCCCCC CGAGCCCAGC 
GAGGCCCCCT GCGCTGAGGA CGAGTGCTTC TCCGGCGACG GCACCACGCC GCTGCCCACG 
CTGGCCCCGC AGGCCGAGGC CCGCCCGCTC ATCAAGGTCA AGCAGCTGAC GCAGAACTCG 
GCCACCATCA CGGTGCAGCT GCCCAGCCCC TTCACCCGCA TGTACACGCT GGAGCACTTC 
AACAACAGCA AGTCGTCCAC CGTGTCCAGG CTGACCCGGG CCCAGGAGGA GATCCGCCTG 
ACCAACCTGT ACGCGCTCAC CAACTACACC TATTGTGTGG TGTCCAGCAG CTCGGGCCTG 
CACCACAACC ACACCTGCCT CACCATCTGC CTGCCGCAGC CGCCTAGCCC GCCGGGCCCC 
GTGCCCAGCC CCTCCACGGC CACCCACTAC ATCATGACCG TCCTGGGCTG CCTCTTCGGC 
ATGGTGGTGG TGCTGGGCGC CGTCTACTAC TGCCTGCGGC GGCGGCGGCG GCGGGAGGGC 
AAGCAGAAGC AGGCGGCGGC AGCGGCCGGC AGCCTCAAGA AGACCATCAT CGAGCTCAAG 
TACGGGCCTG AGCTGGAGGC GCCAGGCCTG GCCCCGCTGT CCCAGGGCCC GCTGCTGGGC 
CCCGAGGCGG TGACCCGCAT CCCATACCTG CCCGCCGCCC CCGGGGAGGT GGAGCAGTAC 
CAGCTGGCGG AGGGCAGCGG GACACCCAAG GGCAGCAAGG GCGGCTACAT GGAGGTGCGT 
GCGGCAGAGC AGGCAGAACG CAGGGAGGGG GAGCAGGGCC GGCCGGGCCC CGACAGCCAG 
AGCTCGGTGG CTGAGATCTC CACCATCGCC AAGGAGGTGG ACAAGGTCAA CCAGATCATC 
AACAACTGCA TCGACGCCCT CAAGGCCGAG GCCACCTCCT TCCAGGGCGG CAAGCCGGGC 
GCCGTGTCCA CGGCCGAGCC GCTGGCCGGC AAGCGCGGCT TCCTGGCGCC CGCCTACCAG 
GACGCCTGCG GCCTGCAGCG GCACCACAGC CTGGAGGCCG CCCCCGGGGC CCCGCGGGCC 
AGCTCCTCGT CCAGCGGCTC CGCCCGCAGC CCGCGGCCCT ACCGCGCCGA GCCCCCTGCG 
CTGCACGAGG CCAAGTACAT CGAGAAGGGC TCGCCCGCGG CGGAGGCCAT CCTCACGGTG 
ACGCCCGCGG CAGCCGTGCT GCGGCCCGAG CCCGAGAAGG GCCGGCAGTA CGGGGAGCAC 
CGGCACTCGT ACCCCGGGCC CCACCCTGTG GAGCCCCCCG CGCCGCCCGC GCCCCCCGAG 
AGCCTGGGCG GCCGCAAGGC CTCCATCCTG GAGCCGCTGA CGCGGCCGCG CCCCCGCGAC 
CTGGCCTACG CGCCGCTCTC GCCGCAGTAC CGCCACCTGA GCTACGCCTC CAGCCCCGAG 
TACGCCTGCC GCGCCTCCCG CAGCCTCTGG GCCCGCTTCA GACTGAGCCG CCCGCGGCAC 
CGGGACGCCG GGGAGTGCGT GGCGGCCGGC CACGCGCTGC GCCGGAAGGT GCAGTTCGCC 
AAGGACGAGG ACCTGCACGA CATCCTGGAC TACTGGAAGG GCGTGTCTGC GCAGCACAAG 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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ELFN1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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