RGS22 cDNA ORF clone, Bos taurus(cattle)

The following RGS22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RGS22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa56232 XM_025001877.1
Latest version!
Bos taurus regulator of G protein signaling 22 (RGS22), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa92564 XM_015474518.2
Latest version!
Bos taurus regulator of G protein signaling 22 (RGS22), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa92564 XM_015466376.1
Latest version!
Bos taurus regulator of G-protein signaling 22 (RGS22), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa07980 XM_005215633.4
Latest version!
Bos taurus regulator of G protein signaling 22 (RGS22), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa07980 XM_015466375.1
Latest version!
Bos taurus regulator of G-protein signaling 22 (RGS22), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa26089 XM_015466374.1
Latest version!
Bos taurus regulator of G-protein signaling 22 (RGS22), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa26089 XM_002692801.5
Latest version!
Bos taurus regulator of G protein signaling 22 (RGS22), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OBa26089 XM_002692801.4 Bos taurus regulator of G-protein signaling 22 (RGS22), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa92564 XM_015474518.1 Bos taurus regulator of G-protein signaling 22 (RGS22), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa07980 XM_005215633.3 Bos taurus regulator of G-protein signaling 22 (RGS22), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa56232
    Clone ID Related Accession (Same CDS sequence) XM_025001877.1
    Accession Version XM_025001877.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3675bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulator of G-protein signaling 22 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGTC TATGGAGTTT TTGAAGTAGT TAACAATGCT
    CCACAACTTC TGGAAAAGCA GTTGAAAAAA ATTGTACTAA ACCAGCGACC TCGAAATCCT
    ATCTATGATG TTGTAAGGAA AGGAAAGAGT GATGTTAAAC CTGTTCAAGT GAACGCCCCT
    CACGAAGATG AGACCATTAA TGTCAACTAC AATATCATGT GTCTCAATCG TGAACAAGGT
    ATTCACTGGG TCAAAAAAGA AAGACTTCCA ACTTTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGGTTAG CCAAATTGAT TTCACAAGTA AGATGGAGTG AATCAGGCAT GAATATCATA
    TTAGGTACAG AGTTTTCACC CTGGGTCCTG AAAAAACCAC CCAGTCCACC ACCTCCTCCT
    GTTGAAGAAG ATGATGTTAT AATTATGAAG AAGTTCTACA TTTCTCTGGG CGAGGCCTCC
    TATACTCAAA CAAAAGATTG GTTTGCCTTA GCAAAACAAT GTCAGCAAAC AGTACCAACC
    TTTTCACTAC CATCTTGTGT ATTCCACAAC AAAATTGAAT CACCAGTTGT TTCATCTGCT
    TCTGAGAGTT TTATCTTCGA TGATGGAGTT CACCCCAGGA CGAAAAAGGG CCTATTAAAA
    ACCACTAGAT TAACCTCCGA ATTTGAAGAA GATGGGTCTG TATCTCTACA GGACACTCCT
    TCTCAAGCTC TTCTGAGGAT ATACCTTGAA AAGCAACAGA ATGTTGATGG AAGTCTGACA
    CTGCACTTCT CAACATGTGA CGAGTTTTTA AGATCTTATG TCTCCTTTAT CTTGAGAGTG
    GCGATTCATC AAATTCTTGG AGCAACACTT AGAGAAAGCC CAGATTACGT AAATTTCAGA
    AACGTAACAG AAGTGGTATT TGATGACATT GGGGGGCCTA CCTCTGGCAA GAATGTTTTG
    TTCAATGAAA TGCTAAGGGA AGAGTCGGGA GACATGTTAG AACCAGCAGT TTCAAAGCCT
    GACAGCATTG GACTCGACAG CAGGGCTGAT TGGTGTATTT CTCACAAAAC TTATGACATT
    GGTAATAGAA AGGAGTTTGA AAGATTTAAG AAATTTTTAA AAGGTACCTT AGGGGAGAAA
    TATTGGTGGC TATGGATGGA TATTGAGAGG TTAAAAGTTC TTAAGAATCC TGGAAGACAT
    CAAAGACATA TTGAGAAGAT GAAAAAATGC TTTCTAGTGA GCAGTGGAGA TTACTACCTT
    TCTGCAGAAG TCTTATCAAA ATTTAAACTA CTAGATGGAT CTCAGTGGAA TGAAGAACAC
    TTAAAAAAAA TTCAGACTGA GGTTTTAAAG CCCTTACTTC TTTATTGGGC ACCAAGATTC
    TGTGTTACTC ACTCATCCTC AACAAAACAT GCTAGTGCCG AGCTGAAATT CTGGCACCTT
    CGTCAGGAGC AGCCGAGGAA GGATGTTGAC CCTTTCCCAC AAATGGCAAC CCTCTTGCCG
    TTAAGACCCA AATCTTGCAT TCCACAGCTA CCTGAGATGC AGAAAGAAGA ATCCAGTTTA
    CTACAACTTC CTAAATCTCC CAAGAAATCA CCTAGAGTAA AGACAGCAGT TTATAAACCT
    TGGAAGAGTG AGTCTTTGAA TCCAGTTTCT TCTAAGGATG ATGTGATTGA GAAAGGGTCA
    AGGCACATGT CAGAAAGCAG CAAAGTTATT CGTTTAACAT CTTTTACTGA CATTTCTGAA
    TGTCTGAAGC CCCAGCTGGA TAGAAGATAT ACCTATACAG AAGAACCTTT GATTAAAACC
    ATGGGTGATG GTACCTTGGG AGGATTTGAC ATGGAGAATT TGTTGCAATC TTTGTATGTA
    GAAAACAGAG CTGGATTCTT CTTTACTAAA TTCTGCGAGA ACTCAGGGAA CAAGTTGTGG
    AAGAACTGTG TGTACTTTTG GTTTGACCTA CAAGCTTATC ATCAGCTTTT CTACCAAGAA
    ACCCTTCACC CTTTTAAAGT ATGCAAGCAA GCTCAATATC TTTTTGCTAC ATACATTGCT
    CCTTCAGCCA CTCTTGACAT TGGACTTCAA CAGGAAAAGA AAAGAGAAAT TTATATGAAA
    ATACAACCAC CATTTGAAGA CCTTTTTGAT GCAGCAGAAG AATATATCCT CCTCCTCCTT
    CTTGAGCCTT GGACAAAGAT GATAAAATCA GACCAAGTTG CTTACAGAAA GGTGGAGCTG
    GTGGAAGAAA CTCGACAGCT GGATTCCACA TACTTGAGAA AGCTACAGGC TTTGCATAAA
    GAGACAGTTT TCAAGAAAGC TGAGGACACT ACTTCTGAAA TTGGAACTGG TATTTCACCA
    CTTCCTCATG TCTCTAAAAA CACAGAATAC TGGAATATGG TTCCTGAAGA ATATAGGCAT
    TTTAATTTTA ATGAACTGCT TAACAACAAA CTGGAGTTTG AACATTTCCG TCAGTTTCTT
    GAGGCTCATT CTTCAAGCAT GGACCTTATG TGCTGGACAG ACATTGAACA GTTCCGAAGA
    ATAACTTACA GAGATCGAAA GCAAAGGGAG GCAAAATCTA TATATATAAA AAACAAATAC
    CTCAATAAAA AGTATTTCTT TGGGCCCAAC AGTCCAGCTT CTGTGTATCA ACAGGACCAG
    ATAATGAATT TAAGTGGTGG CTGGGGAAAG ATTCTCCATG AGCAACTTGA TGCAGCTGTT
    CTAATCGAAA TTCAGAAGCA TGTCCTAAAT AGACTAGAAA ATGTTTGGTT GCCGTTGTTT
    CTTGCAAGTG AACAGTTTGC GGCACGTCAA AAGATAAAGG TACAGATAAA AGACATAGCT
    GAAGAGCTTT TACTACAAAA GCGTGAAAGG AAAATTGGAG TCTGGAAGCC TGTGGAGAGT
    AAGTGGATAT CTTCGTCTTG TGAAATCATT GCTTTCCGTA AAGCTTTATT GAATCCAGTT
    ACTGCAAGAC AATTTCAACG CTTTGTGGCT CTAAAAGGAG ATTTATTGGA AAATGGGGTG
    CTCTTTTGGC AAGAAGTACA AAAATATAAG GACTTGTGCC ATTCTCATTG TGATGAGTCT
    ATCATACACA AGAAGATTAT GACTATTATC AACTGCTTTA TTAATTCCAG CATCCCCCCA
    GCTTTACAAA TTGACATTCC AGTAGAGCAA GCCCAGAAGA TTATTGAACA CAGGAAGGAG
    TTGGGACCAT ATGTATTCAG AGAGGCACAG ATGACAATTT TTGGAGTTCT GTTTAAATTT
    TGGCCTCAGT TCTGTGAGTT TAGGAAGAAC TTAACTGATG AAAAAATTAT GAGTGTTTTG
    GAGAGAAGAC AAGAATATAG TAAGCAGAAA AAGAAAATGG CAGTCATAGA AGATGAAAAA
    TTTGTAAAGC CAACCTGGTG CTACTCAAAG TACATAGAAG CCTTAGAACA GGAAAGGATT
    CTTCTTAAAA TTCAAGAGGA ACTAGAAAGA AAGTTGTTTG CAGCCACACC ATCTTTTACA
    AATTTCAAGT CTACTGCTTC CACTCTGTCT TTGAAAAAGA CTGTGTCCTC CCACAACATC
    CAGAGAATTC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857645.1
    CDS77..3751
    Translation

    Target ORF information:

    RefSeq Version XM_025001877.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulator of G protein signaling 22 (RGS22), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001877.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGTC TATGGAGTTT TTGAAGTAGT TAACAATGCT
    CCACAACTTC TGGAAAAGCA GTTGAAAAAA ATTGTACTAA ACCAGCGACC TCGAAATCCT
    ATCTATGATG TTGTAAGGAA AGGAAAGAGT GATGTTAAAC CTGTTCAAGT GAACGCCCCT
    CACGAAGATG AGACCATTAA TGTCAACTAC AATATCATGT GTCTCAATCG TGAACAAGGT
    ATTCACTGGG TCAAAAAAGA AAGACTTCCA ACTTTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGGTTAG CCAAATTGAT TTCACAAGTA AGATGGAGTG AATCAGGCAT GAATATCATA
    TTAGGTACAG AGTTTTCACC CTGGGTCCTG AAAAAACCAC CCAGTCCACC ACCTCCTCCT
    GTTGAAGAAG ATGATGTTAT AATTATGAAG AAGTTCTACA TTTCTCTGGG CGAGGCCTCC
    TATACTCAAA CAAAAGATTG GTTTGCCTTA GCAAAACAAT GTCAGCAAAC AGTACCAACC
    TTTTCACTAC CATCTTGTGT ATTCCACAAC AAAATTGAAT CACCAGTTGT TTCATCTGCT
    TCTGAGAGTT TTATCTTCGA TGATGGAGTT CACCCCAGGA CGAAAAAGGG CCTATTAAAA
    ACCACTAGAT TAACCTCCGA ATTTGAAGAA GATGGGTCTG TATCTCTACA GGACACTCCT
    TCTCAAGCTC TTCTGAGGAT ATACCTTGAA AAGCAACAGA ATGTTGATGG AAGTCTGACA
    CTGCACTTCT CAACATGTGA CGAGTTTTTA AGATCTTATG TCTCCTTTAT CTTGAGAGTG
    GCGATTCATC AAATTCTTGG AGCAACACTT AGAGAAAGCC CAGATTACGT AAATTTCAGA
    AACGTAACAG AAGTGGTATT TGATGACATT GGGGGGCCTA CCTCTGGCAA GAATGTTTTG
    TTCAATGAAA TGCTAAGGGA AGAGTCGGGA GACATGTTAG AACCAGCAGT TTCAAAGCCT
    GACAGCATTG GACTCGACAG CAGGGCTGAT TGGTGTATTT CTCACAAAAC TTATGACATT
    GGTAATAGAA AGGAGTTTGA AAGATTTAAG AAATTTTTAA AAGGTACCTT AGGGGAGAAA
    TATTGGTGGC TATGGATGGA TATTGAGAGG TTAAAAGTTC TTAAGAATCC TGGAAGACAT
    CAAAGACATA TTGAGAAGAT GAAAAAATGC TTTCTAGTGA GCAGTGGAGA TTACTACCTT
    TCTGCAGAAG TCTTATCAAA ATTTAAACTA CTAGATGGAT CTCAGTGGAA TGAAGAACAC
    TTAAAAAAAA TTCAGACTGA GGTTTTAAAG CCCTTACTTC TTTATTGGGC ACCAAGATTC
    TGTGTTACTC ACTCATCCTC AACAAAACAT GCTAGTGCCG AGCTGAAATT CTGGCACCTT
    CGTCAGGAGC AGCCGAGGAA GGATGTTGAC CCTTTCCCAC AAATGGCAAC CCTCTTGCCG
    TTAAGACCCA AATCTTGCAT TCCACAGCTA CCTGAGATGC AGAAAGAAGA ATCCAGTTTA
    CTACAACTTC CTAAATCTCC CAAGAAATCA CCTAGAGTAA AGACAGCAGT TTATAAACCT
    TGGAAGAGTG AGTCTTTGAA TCCAGTTTCT TCTAAGGATG ATGTGATTGA GAAAGGGTCA
    AGGCACATGT CAGAAAGCAG CAAAGTTATT CGTTTAACAT CTTTTACTGA CATTTCTGAA
    TGTCTGAAGC CCCAGCTGGA TAGAAGATAT ACCTATACAG AAGAACCTTT GATTAAAACC
    ATGGGTGATG GTACCTTGGG AGGATTTGAC ATGGAGAATT TGTTGCAATC TTTGTATGTA
    GAAAACAGAG CTGGATTCTT CTTTACTAAA TTCTGCGAGA ACTCAGGGAA CAAGTTGTGG
    AAGAACTGTG TGTACTTTTG GTTTGACCTA CAAGCTTATC ATCAGCTTTT CTACCAAGAA
    ACCCTTCACC CTTTTAAAGT ATGCAAGCAA GCTCAATATC TTTTTGCTAC ATACATTGCT
    CCTTCAGCCA CTCTTGACAT TGGACTTCAA CAGGAAAAGA AAAGAGAAAT TTATATGAAA
    ATACAACCAC CATTTGAAGA CCTTTTTGAT GCAGCAGAAG AATATATCCT CCTCCTCCTT
    CTTGAGCCTT GGACAAAGAT GATAAAATCA GACCAAGTTG CTTACAGAAA GGTGGAGCTG
    GTGGAAGAAA CTCGACAGCT GGATTCCACA TACTTGAGAA AGCTACAGGC TTTGCATAAA
    GAGACAGTTT TCAAGAAAGC TGAGGACACT ACTTCTGAAA TTGGAACTGG TATTTCACCA
    CTTCCTCATG TCTCTAAAAA CACAGAATAC TGGAATATGG TTCCTGAAGA ATATAGGCAT
    TTTAATTTTA ATGAACTGCT TAACAACAAA CTGGAGTTTG AACATTTCCG TCAGTTTCTT
    GAGGCTCATT CTTCAAGCAT GGACCTTATG TGCTGGACAG ACATTGAACA GTTCCGAAGA
    ATAACTTACA GAGATCGAAA GCAAAGGGAG GCAAAATCTA TATATATAAA AAACAAATAC
    CTCAATAAAA AGTATTTCTT TGGGCCCAAC AGTCCAGCTT CTGTGTATCA ACAGGACCAG
    ATAATGAATT TAAGTGGTGG CTGGGGAAAG ATTCTCCATG AGCAACTTGA TGCAGCTGTT
    CTAATCGAAA TTCAGAAGCA TGTCCTAAAT AGACTAGAAA ATGTTTGGTT GCCGTTGTTT
    CTTGCAAGTG AACAGTTTGC GGCACGTCAA AAGATAAAGG TACAGATAAA AGACATAGCT
    GAAGAGCTTT TACTACAAAA GCGTGAAAGG AAAATTGGAG TCTGGAAGCC TGTGGAGAGT
    AAGTGGATAT CTTCGTCTTG TGAAATCATT GCTTTCCGTA AAGCTTTATT GAATCCAGTT
    ACTGCAAGAC AATTTCAACG CTTTGTGGCT CTAAAAGGAG ATTTATTGGA AAATGGGGTG
    CTCTTTTGGC AAGAAGTACA AAAATATAAG GACTTGTGCC ATTCTCATTG TGATGAGTCT
    ATCATACACA AGAAGATTAT GACTATTATC AACTGCTTTA TTAATTCCAG CATCCCCCCA
    GCTTTACAAA TTGACATTCC AGTAGAGCAA GCCCAGAAGA TTATTGAACA CAGGAAGGAG
    TTGGGACCAT ATGTATTCAG AGAGGCACAG ATGACAATTT TTGGAGTTCT GTTTAAATTT
    TGGCCTCAGT TCTGTGAGTT TAGGAAGAAC TTAACTGATG AAAAAATTAT GAGTGTTTTG
    GAGAGAAGAC AAGAATATAG TAAGCAGAAA AAGAAAATGG CAGTCATAGA AGATGAAAAA
    TTTGTAAAGC CAACCTGGTG CTACTCAAAG TACATAGAAG CCTTAGAACA GGAAAGGATT
    CTTCTTAAAA TTCAAGAGGA ACTAGAAAGA AAGTTGTTTG CAGCCACACC ATCTTTTACA
    AATTTCAAGT CTACTGCTTC CACTCTGTCT TTGAAAAAGA CTGTGTCCTC CCACAACATC
    CAGAGAATTC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92564
    Clone ID Related Accession (Same CDS sequence) XM_015466376.1 , XM_015474518.1 , XM_015474518.2
    Accession Version XM_015466376.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulator of G-protein signaling 22 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646230.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCATGT
    CTCAATCGTG AACAAGGTAT TCACTGGGTC AAAAAAGAAA GACTTCCAAC TTTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGGTTAGCC AAATTGATTT CACAAGTAAG ATGGAGTGAA
    TCAGGCATGA ATATCATATT AGGTACAGAG TTTTCACCCT GGGTCCTGAA AAAACCACCC
    AGTCCACCAC CTCCTCCTGT TGAAGAAGAT GATGTTATAA TTATGAAGAA GTTCTACATT
    TCTCTGGGCG AGGCCTCCTA TACTCAAACA AAAGATTGGT TTGCCTTAGC AAAACAATGT
    CAGCAAACAG TACCAACCTT TTCACTACCA TCTTGTGTAT TCCACAACAA AATTGAATCA
    CCAGTTGTTT CATCTGCTTC TGAGAGTTTT ATCTTCGATG ATGGAGTTCA CCCCAGGACG
    AAAAAGGGCC TATTAAAAAC CACTAGATTA ACCTCCGAAT TTGAAGAAGA TGGGTCTGTA
    TCTCTACAGG ACACTCCTTC TCAAGCTCTT CTGAGGATAT ACCTTGAAAA GCAACAGAAT
    GTTGATGGAA GTCTGACACT GCACTTCTCA ACATGTGACG AGTTTTTAAG ATCTTATGTC
    TCCTTTATCT TGAGAGTGGC GATTCATCAA ATTCTTGGAG CAACACTTAG AGAAAGCCCA
    GATTACGTAA ATTTCAGAAA CGTAACAGAA GTGGTATTTG ATGACATTGG GGGGCCTACC
    TCTGGCAAGA ATGTTTTGTT CAATGAAATG CTAAGGGAAG AGTCGGGAGA CATGTTAGAA
    CCAGCAGTTT CAAAGCCTGA CAGCATTGGA CTCGACAGCA GGGCTGATTG GTGTATTTCT
    CACAAAACTT ATGACATTGG TAATAGAAAG GAGTTTGAAA GATTTAAGAA ATTTTTAAAA
    GGTACCTTAG GGGAGAAATA TTGGTGGCTA TGGATGGATA TTGAGAGGTT AAAAGTTCTT
    AAGAATCCTG GAAGACATCA AAGACATATT GAGAAGATGA AAAAATGCTT TCTAGTGAGC
    AGTGGAGATT ACTACCTTTC TGCAGAAGTC TTATCAAAAT TTAAACTACT AGATGGATCT
    CAGTGGAATG AAGAACACTT AAAAAAAATT CAGACTGAGG TTTTAAAGCC CTTACTTCTT
    TATTGGGCAC CAAGATTCTG TGTTACTCAC TCATCCTCAA CAAAACATGC TAGTGCCGAG
    CTGAAATTCT GGCACCTTCG TCAGGAGCAG CCGAGGAAGG ATGTTGACCC TTTCCCACAA
    ATGGCAACCC TCTTGCCGTT AAGACCCAAA TCTTGCATTC CACAGCTACC TGAGATGCAG
    AAAGAAGAAT CCAGTTTACT ACAACTTCCT AAATCTCCCA AGAAATCACC TAGAGTAAAG
    ACAGCAGTTT ATAAACCTTG GAAGAGTGAG TCTTTGAATC CAGTTTCTTC TAAGGATGAT
    GTGATTGAGA AAGGGTCAAG GCACATGTCA GAAAGCAGCA AAGTTATTCG TTTAACATCT
    TTTACTGACA TTTCTGAATG TCTGAAGCCC CAGCTGGATA GAAGATATAC CTATACAGAA
    GAACCTTTGA TTAAAACCAT GGGTGATGGT ACCTTGGGAG GATTTGACAT GGAGAATTTG
    TTGCAATCTT TGTATGTAGA AAACAGAGCT GGATTCTTCT TTACTAAATT CTGCGAGAAC
    TCAGGGAACA AGTTGTGGAA GAACTGTGTG TACTTTTGGT TTGACCTACA AGCTTATCAT
    CAGCTTTTCT ACCAAGAAAC CCTTCACCCT TTTAAAGTAT GCAAGCAAGC TCAATATCTT
    TTTGCTACAT ACATTGCTCC TTCAGCCACT CTTGACATTG GACTTCAACA GGAAAAGAAA
    AGAGAAATTT ATATGAAAAT ACAACCACCA TTTGAAGACC TTTTTGATGC AGCAGAAGAA
    TATATCCTCC TCCTCCTTCT TGAGCCTTGG ACAAAGATGA TAAAATCAGA CCAAGTTGCT
    TACAGAAAGG TGGAGCTGGT GGAAGAAACT CGACAGCTGG ATTCCACATA CTTGAGAAAG
    CTACAGGCTT TGCATAAAGA GACAGTTTTC AAGAAAGCTG AGGACACTAC TTCTGAAATT
    GGAACTGGTA TTTCACCACT TCCTCATGTC TCTAAAAACA CAGAATACTG GAATATGGTT
    CCTGAAGAAT ATAGGCATTT TAATTTTAAT GAACTGCTTA ACAACAAACT GGAGTTTGAA
    CATTTCCGTC AGTTTCTTGA GGCTCATTCT TCAAGCATGG ACCTTATGTG CTGGACAGAC
    ATTGAACAGT TCCGAAGAAT AACTTACAGA GATCGAAAGC AAAGGGAGGC AAAATCTATA
    TATATAAAAA ACAAATACCT CAATAAAAAG TATTTCTTTG GGCCCAACAG TCCAGCTTCT
    GTGTATCAAC AGGACCAGAT AATGAATTTA AGTGGTGGCT GGGGAAAGAT TCTCCATGAG
    CAACTTGATG CAGCTGTTCT AATCGAAATT CAGAAGCATG TCCTAAATAG ACTAGAAAAT
    GTTTGGTTGC CGTTGTTTCT TGCAAGTGAA CAGTTTGCGG CACGTCAAAA GATAAAGGTA
    CAGATAAAAG ACATAGCTGA AGAGCTTTTA CTACAAAAGC GTGAAAGGAA AATTGGAGTC
    TGGAAGCCTG TGGAGAGTAA GTGGATATCT TCGTCTTGTG AAATCATTGC TTTCCGTAAA
    GCTTTATTGA ATCCAGTTAC TGCAAGACAA TTTCAACGCT TTGTGGCTCT AAAAGGAGAT
    TTATTGGAAA ATGGGGTGCT CTTTTGGCAA GAAGTACAAA AATATAAGGA CTTGTGCCAT
    TCTCATTGTG ATGAGTCTAT CATACACAAG AAGATTATGA CTATTATCAA CTGCTTTATT
    AATTCCAGCA TCCCCCCAGC TTTACAAATT GACATTCCAG TAGAGCAAGC CCAGAAGATT
    ATTGAACACA GGAAGGAGTT GGGACCATAT GTATTCAGAG AGGCACAGAT GACAATTTTT
    GGAGTTCTGT TTAAATTTTG GCCTCAGTTC TGTGAGTTTA GGAAGAACTT AACTGATGAA
    AAAATTATGA GTGTTTTGGA GAGAAGACAA GAATATAGTA AGCAGAAAAA GAAAATGGCA
    GTCATAGAAG ATGAAAAATT TGTAAAGGAT GGAATCAAAC AATATTCGAG TACTTCAGGA
    TCTGTTATCA AAGCAGCTGC TTTAACTAGT GACCCAGGTT TAGGCATGCA AGCGTACGGC
    CGACAGCCAA CCTGGTGCTA CTCAAAGTAC ATAGAAGCCT TAGAACAGGA AAGGATTCTT
    CTTAAAATTC AAGAGGAACT AGAAAGAAAG TTGTTTGCAG CCACACCATC TTTTACAAAT
    TTCAAGTCTA CTGCTTCCAC TCTGTCTTTG AAAAAGACTG TGTCCTCCCA CAACATCCAG
    AGAATTCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321862.1
    CDS46..3597
    Translation

    Target ORF information:

    RefSeq Version XM_015466376.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulator of G-protein signaling 22 (RGS22), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466376.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCATGT
    CTCAATCGTG AACAAGGTAT TCACTGGGTC AAAAAAGAAA GACTTCCAAC TTTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGGTTAGCC AAATTGATTT CACAAGTAAG ATGGAGTGAA
    TCAGGCATGA ATATCATATT AGGTACAGAG TTTTCACCCT GGGTCCTGAA AAAACCACCC
    AGTCCACCAC CTCCTCCTGT TGAAGAAGAT GATGTTATAA TTATGAAGAA GTTCTACATT
    TCTCTGGGCG AGGCCTCCTA TACTCAAACA AAAGATTGGT TTGCCTTAGC AAAACAATGT
    CAGCAAACAG TACCAACCTT TTCACTACCA TCTTGTGTAT TCCACAACAA AATTGAATCA
    CCAGTTGTTT CATCTGCTTC TGAGAGTTTT ATCTTCGATG ATGGAGTTCA CCCCAGGACG
    AAAAAGGGCC TATTAAAAAC CACTAGATTA ACCTCCGAAT TTGAAGAAGA TGGGTCTGTA
    TCTCTACAGG ACACTCCTTC TCAAGCTCTT CTGAGGATAT ACCTTGAAAA GCAACAGAAT
    GTTGATGGAA GTCTGACACT GCACTTCTCA ACATGTGACG AGTTTTTAAG ATCTTATGTC
    TCCTTTATCT TGAGAGTGGC GATTCATCAA ATTCTTGGAG CAACACTTAG AGAAAGCCCA
    GATTACGTAA ATTTCAGAAA CGTAACAGAA GTGGTATTTG ATGACATTGG GGGGCCTACC
    TCTGGCAAGA ATGTTTTGTT CAATGAAATG CTAAGGGAAG AGTCGGGAGA CATGTTAGAA
    CCAGCAGTTT CAAAGCCTGA CAGCATTGGA CTCGACAGCA GGGCTGATTG GTGTATTTCT
    CACAAAACTT ATGACATTGG TAATAGAAAG GAGTTTGAAA GATTTAAGAA ATTTTTAAAA
    GGTACCTTAG GGGAGAAATA TTGGTGGCTA TGGATGGATA TTGAGAGGTT AAAAGTTCTT
    AAGAATCCTG GAAGACATCA AAGACATATT GAGAAGATGA AAAAATGCTT TCTAGTGAGC
    AGTGGAGATT ACTACCTTTC TGCAGAAGTC TTATCAAAAT TTAAACTACT AGATGGATCT
    CAGTGGAATG AAGAACACTT AAAAAAAATT CAGACTGAGG TTTTAAAGCC CTTACTTCTT
    TATTGGGCAC CAAGATTCTG TGTTACTCAC TCATCCTCAA CAAAACATGC TAGTGCCGAG
    CTGAAATTCT GGCACCTTCG TCAGGAGCAG CCGAGGAAGG ATGTTGACCC TTTCCCACAA
    ATGGCAACCC TCTTGCCGTT AAGACCCAAA TCTTGCATTC CACAGCTACC TGAGATGCAG
    AAAGAAGAAT CCAGTTTACT ACAACTTCCT AAATCTCCCA AGAAATCACC TAGAGTAAAG
    ACAGCAGTTT ATAAACCTTG GAAGAGTGAG TCTTTGAATC CAGTTTCTTC TAAGGATGAT
    GTGATTGAGA AAGGGTCAAG GCACATGTCA GAAAGCAGCA AAGTTATTCG TTTAACATCT
    TTTACTGACA TTTCTGAATG TCTGAAGCCC CAGCTGGATA GAAGATATAC CTATACAGAA
    GAACCTTTGA TTAAAACCAT GGGTGATGGT ACCTTGGGAG GATTTGACAT GGAGAATTTG
    TTGCAATCTT TGTATGTAGA AAACAGAGCT GGATTCTTCT TTACTAAATT CTGCGAGAAC
    TCAGGGAACA AGTTGTGGAA GAACTGTGTG TACTTTTGGT TTGACCTACA AGCTTATCAT
    CAGCTTTTCT ACCAAGAAAC CCTTCACCCT TTTAAAGTAT GCAAGCAAGC TCAATATCTT
    TTTGCTACAT ACATTGCTCC TTCAGCCACT CTTGACATTG GACTTCAACA GGAAAAGAAA
    AGAGAAATTT ATATGAAAAT ACAACCACCA TTTGAAGACC TTTTTGATGC AGCAGAAGAA
    TATATCCTCC TCCTCCTTCT TGAGCCTTGG ACAAAGATGA TAAAATCAGA CCAAGTTGCT
    TACAGAAAGG TGGAGCTGGT GGAAGAAACT CGACAGCTGG ATTCCACATA CTTGAGAAAG
    CTACAGGCTT TGCATAAAGA GACAGTTTTC AAGAAAGCTG AGGACACTAC TTCTGAAATT
    GGAACTGGTA TTTCACCACT TCCTCATGTC TCTAAAAACA CAGAATACTG GAATATGGTT
    CCTGAAGAAT ATAGGCATTT TAATTTTAAT GAACTGCTTA ACAACAAACT GGAGTTTGAA
    CATTTCCGTC AGTTTCTTGA GGCTCATTCT TCAAGCATGG ACCTTATGTG CTGGACAGAC
    ATTGAACAGT TCCGAAGAAT AACTTACAGA GATCGAAAGC AAAGGGAGGC AAAATCTATA
    TATATAAAAA ACAAATACCT CAATAAAAAG TATTTCTTTG GGCCCAACAG TCCAGCTTCT
    GTGTATCAAC AGGACCAGAT AATGAATTTA AGTGGTGGCT GGGGAAAGAT TCTCCATGAG
    CAACTTGATG CAGCTGTTCT AATCGAAATT CAGAAGCATG TCCTAAATAG ACTAGAAAAT
    GTTTGGTTGC CGTTGTTTCT TGCAAGTGAA CAGTTTGCGG CACGTCAAAA GATAAAGGTA
    CAGATAAAAG ACATAGCTGA AGAGCTTTTA CTACAAAAGC GTGAAAGGAA AATTGGAGTC
    TGGAAGCCTG TGGAGAGTAA GTGGATATCT TCGTCTTGTG AAATCATTGC TTTCCGTAAA
    GCTTTATTGA ATCCAGTTAC TGCAAGACAA TTTCAACGCT TTGTGGCTCT AAAAGGAGAT
    TTATTGGAAA ATGGGGTGCT CTTTTGGCAA GAAGTACAAA AATATAAGGA CTTGTGCCAT
    TCTCATTGTG ATGAGTCTAT CATACACAAG AAGATTATGA CTATTATCAA CTGCTTTATT
    AATTCCAGCA TCCCCCCAGC TTTACAAATT GACATTCCAG TAGAGCAAGC CCAGAAGATT
    ATTGAACACA GGAAGGAGTT GGGACCATAT GTATTCAGAG AGGCACAGAT GACAATTTTT
    GGAGTTCTGT TTAAATTTTG GCCTCAGTTC TGTGAGTTTA GGAAGAACTT AACTGATGAA
    AAAATTATGA GTGTTTTGGA GAGAAGACAA GAATATAGTA AGCAGAAAAA GAAAATGGCA
    GTCATAGAAG ATGAAAAATT TGTAAAGGAT GGAATCAAAC AATATTCGAG TACTTCAGGA
    TCTGTTATCA AAGCAGCTGC TTTAACTAGT GACCCAGGTT TAGGCATGCA AGCGTACGGC
    CGACAGCCAA CCTGGTGCTA CTCAAAGTAC ATAGAAGCCT TAGAACAGGA AAGGATTCTT
    CTTAAAATTC AAGAGGAACT AGAAAGAAAG TTGTTTGCAG CCACACCATC TTTTACAAAT
    TTCAAGTCTA CTGCTTCCAC TCTGTCTTTG AAAAAGACTG TGTCCTCCCA CAACATCCAG
    AGAATTCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92564
    Clone ID Related Accession (Same CDS sequence) XM_015466376.1 , XM_015474518.1 , XM_015474518.2
    Accession Version XM_015474518.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulator of G-protein signaling 22 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104399.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCATGT
    CTCAATCGTG AACAAGGTAT TCACTGGGTC AAAAAAGAAA GACTTCCAAC TTTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGGTTAGCC AAATTGATTT CACAAGTAAG ATGGAGTGAA
    TCAGGCATGA ATATCATATT AGGTACAGAG TTTTCACCCT GGGTCCTGAA AAAACCACCC
    AGTCCACCAC CTCCTCCTGT TGAAGAAGAT GATGTTATAA TTATGAAGAA GTTCTACATT
    TCTCTGGGCG AGGCCTCCTA TACTCAAACA AAAGATTGGT TTGCCTTAGC AAAACAATGT
    CAGCAAACAG TACCAACCTT TTCACTACCA TCTTGTGTAT TCCACAACAA AATTGAATCA
    CCAGTTGTTT CATCTGCTTC TGAGAGTTTT ATCTTCGATG ATGGAGTTCA CCCCAGGACG
    AAAAAGGGCC TATTAAAAAC CACTAGATTA ACCTCCGAAT TTGAAGAAGA TGGGTCTGTA
    TCTCTACAGG ACACTCCTTC TCAAGCTCTT CTGAGGATAT ACCTTGAAAA GCAACAGAAT
    GTTGATGGAA GTCTGACACT GCACTTCTCA ACATGTGACG AGTTTTTAAG ATCTTATGTC
    TCCTTTATCT TGAGAGTGGC GATTCATCAA ATTCTTGGAG CAACACTTAG AGAAAGCCCA
    GATTACGTAA ATTTCAGAAA CGTAACAGAA GTGGTATTTG ATGACATTGG GGGGCCTACC
    TCTGGCAAGA ATGTTTTGTT CAATGAAATG CTAAGGGAAG AGTCGGGAGA CATGTTAGAA
    CCAGCAGTTT CAAAGCCTGA CAGCATTGGA CTCGACAGCA GGGCTGATTG GTGTATTTCT
    CACAAAACTT ATGACATTGG TAATAGAAAG GAGTTTGAAA GATTTAAGAA ATTTTTAAAA
    GGTACCTTAG GGGAGAAATA TTGGTGGCTA TGGATGGATA TTGAGAGGTT AAAAGTTCTT
    AAGAATCCTG GAAGACATCA AAGACATATT GAGAAGATGA AAAAATGCTT TCTAGTGAGC
    AGTGGAGATT ACTACCTTTC TGCAGAAGTC TTATCAAAAT TTAAACTACT AGATGGATCT
    CAGTGGAATG AAGAACACTT AAAAAAAATT CAGACTGAGG TTTTAAAGCC CTTACTTCTT
    TATTGGGCAC CAAGATTCTG TGTTACTCAC TCATCCTCAA CAAAACATGC TAGTGCCGAG
    CTGAAATTCT GGCACCTTCG TCAGGAGCAG CCGAGGAAGG ATGTTGACCC TTTCCCACAA
    ATGGCAACCC TCTTGCCGTT AAGACCCAAA TCTTGCATTC CACAGCTACC TGAGATGCAG
    AAAGAAGAAT CCAGTTTACT ACAACTTCCT AAATCTCCCA AGAAATCACC TAGAGTAAAG
    ACAGCAGTTT ATAAACCTTG GAAGAGTGAG TCTTTGAATC CAGTTTCTTC TAAGGATGAT
    GTGATTGAGA AAGGGTCAAG GCACATGTCA GAAAGCAGCA AAGTTATTCG TTTAACATCT
    TTTACTGACA TTTCTGAATG TCTGAAGCCC CAGCTGGATA GAAGATATAC CTATACAGAA
    GAACCTTTGA TTAAAACCAT GGGTGATGGT ACCTTGGGAG GATTTGACAT GGAGAATTTG
    TTGCAATCTT TGTATGTAGA AAACAGAGCT GGATTCTTCT TTACTAAATT CTGCGAGAAC
    TCAGGGAACA AGTTGTGGAA GAACTGTGTG TACTTTTGGT TTGACCTACA AGCTTATCAT
    CAGCTTTTCT ACCAAGAAAC CCTTCACCCT TTTAAAGTAT GCAAGCAAGC TCAATATCTT
    TTTGCTACAT ACATTGCTCC TTCAGCCACT CTTGACATTG GACTTCAACA GGAAAAGAAA
    AGAGAAATTT ATATGAAAAT ACAACCACCA TTTGAAGACC TTTTTGATGC AGCAGAAGAA
    TATATCCTCC TCCTCCTTCT TGAGCCTTGG ACAAAGATGA TAAAATCAGA CCAAGTTGCT
    TACAGAAAGG TGGAGCTGGT GGAAGAAACT CGACAGCTGG ATTCCACATA CTTGAGAAAG
    CTACAGGCTT TGCATAAAGA GACAGTTTTC AAGAAAGCTG AGGACACTAC TTCTGAAATT
    GGAACTGGTA TTTCACCACT TCCTCATGTC TCTAAAAACA CAGAATACTG GAATATGGTT
    CCTGAAGAAT ATAGGCATTT TAATTTTAAT GAACTGCTTA ACAACAAACT GGAGTTTGAA
    CATTTCCGTC AGTTTCTTGA GGCTCATTCT TCAAGCATGG ACCTTATGTG CTGGACAGAC
    ATTGAACAGT TCCGAAGAAT AACTTACAGA GATCGAAAGC AAAGGGAGGC AAAATCTATA
    TATATAAAAA ACAAATACCT CAATAAAAAG TATTTCTTTG GGCCCAACAG TCCAGCTTCT
    GTGTATCAAC AGGACCAGAT AATGAATTTA AGTGGTGGCT GGGGAAAGAT TCTCCATGAG
    CAACTTGATG CAGCTGTTCT AATCGAAATT CAGAAGCATG TCCTAAATAG ACTAGAAAAT
    GTTTGGTTGC CGTTGTTTCT TGCAAGTGAA CAGTTTGCGG CACGTCAAAA GATAAAGGTA
    CAGATAAAAG ACATAGCTGA AGAGCTTTTA CTACAAAAGC GTGAAAGGAA AATTGGAGTC
    TGGAAGCCTG TGGAGAGTAA GTGGATATCT TCGTCTTGTG AAATCATTGC TTTCCGTAAA
    GCTTTATTGA ATCCAGTTAC TGCAAGACAA TTTCAACGCT TTGTGGCTCT AAAAGGAGAT
    TTATTGGAAA ATGGGGTGCT CTTTTGGCAA GAAGTACAAA AATATAAGGA CTTGTGCCAT
    TCTCATTGTG ATGAGTCTAT CATACACAAG AAGATTATGA CTATTATCAA CTGCTTTATT
    AATTCCAGCA TCCCCCCAGC TTTACAAATT GACATTCCAG TAGAGCAAGC CCAGAAGATT
    ATTGAACACA GGAAGGAGTT GGGACCATAT GTATTCAGAG AGGCACAGAT GACAATTTTT
    GGAGTTCTGT TTAAATTTTG GCCTCAGTTC TGTGAGTTTA GGAAGAACTT AACTGATGAA
    AAAATTATGA GTGTTTTGGA GAGAAGACAA GAATATAGTA AGCAGAAAAA GAAAATGGCA
    GTCATAGAAG ATGAAAAATT TGTAAAGGAT GGAATCAAAC AATATTCGAG TACTTCAGGA
    TCTGTTATCA AAGCAGCTGC TTTAACTAGT GACCCAGGTT TAGGCATGCA AGCGTACGGC
    CGACAGCCAA CCTGGTGCTA CTCAAAGTAC ATAGAAGCCT TAGAACAGGA AAGGATTCTT
    CTTAAAATTC AAGAGGAACT AGAAAGAAAG TTGTTTGCAG CCACACCATC TTTTACAAAT
    TTCAAGTCTA CTGCTTCCAC TCTGTCTTTG AAAAAGACTG TGTCCTCCCA CAACATCCAG
    AGAATTCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330004.1
    CDS46..3597
    Translation

    Target ORF information:

    RefSeq Version XM_015474518.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulator of G-protein signaling 22 (RGS22), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474518.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCATGT
    CTCAATCGTG AACAAGGTAT TCACTGGGTC AAAAAAGAAA GACTTCCAAC TTTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGGTTAGCC AAATTGATTT CACAAGTAAG ATGGAGTGAA
    TCAGGCATGA ATATCATATT AGGTACAGAG TTTTCACCCT GGGTCCTGAA AAAACCACCC
    AGTCCACCAC CTCCTCCTGT TGAAGAAGAT GATGTTATAA TTATGAAGAA GTTCTACATT
    TCTCTGGGCG AGGCCTCCTA TACTCAAACA AAAGATTGGT TTGCCTTAGC AAAACAATGT
    CAGCAAACAG TACCAACCTT TTCACTACCA TCTTGTGTAT TCCACAACAA AATTGAATCA
    CCAGTTGTTT CATCTGCTTC TGAGAGTTTT ATCTTCGATG ATGGAGTTCA CCCCAGGACG
    AAAAAGGGCC TATTAAAAAC CACTAGATTA ACCTCCGAAT TTGAAGAAGA TGGGTCTGTA
    TCTCTACAGG ACACTCCTTC TCAAGCTCTT CTGAGGATAT ACCTTGAAAA GCAACAGAAT
    GTTGATGGAA GTCTGACACT GCACTTCTCA ACATGTGACG AGTTTTTAAG ATCTTATGTC
    TCCTTTATCT TGAGAGTGGC GATTCATCAA ATTCTTGGAG CAACACTTAG AGAAAGCCCA
    GATTACGTAA ATTTCAGAAA CGTAACAGAA GTGGTATTTG ATGACATTGG GGGGCCTACC
    TCTGGCAAGA ATGTTTTGTT CAATGAAATG CTAAGGGAAG AGTCGGGAGA CATGTTAGAA
    CCAGCAGTTT CAAAGCCTGA CAGCATTGGA CTCGACAGCA GGGCTGATTG GTGTATTTCT
    CACAAAACTT ATGACATTGG TAATAGAAAG GAGTTTGAAA GATTTAAGAA ATTTTTAAAA
    GGTACCTTAG GGGAGAAATA TTGGTGGCTA TGGATGGATA TTGAGAGGTT AAAAGTTCTT
    AAGAATCCTG GAAGACATCA AAGACATATT GAGAAGATGA AAAAATGCTT TCTAGTGAGC
    AGTGGAGATT ACTACCTTTC TGCAGAAGTC TTATCAAAAT TTAAACTACT AGATGGATCT
    CAGTGGAATG AAGAACACTT AAAAAAAATT CAGACTGAGG TTTTAAAGCC CTTACTTCTT
    TATTGGGCAC CAAGATTCTG TGTTACTCAC TCATCCTCAA CAAAACATGC TAGTGCCGAG
    CTGAAATTCT GGCACCTTCG TCAGGAGCAG CCGAGGAAGG ATGTTGACCC TTTCCCACAA
    ATGGCAACCC TCTTGCCGTT AAGACCCAAA TCTTGCATTC CACAGCTACC TGAGATGCAG
    AAAGAAGAAT CCAGTTTACT ACAACTTCCT AAATCTCCCA AGAAATCACC TAGAGTAAAG
    ACAGCAGTTT ATAAACCTTG GAAGAGTGAG TCTTTGAATC CAGTTTCTTC TAAGGATGAT
    GTGATTGAGA AAGGGTCAAG GCACATGTCA GAAAGCAGCA AAGTTATTCG TTTAACATCT
    TTTACTGACA TTTCTGAATG TCTGAAGCCC CAGCTGGATA GAAGATATAC CTATACAGAA
    GAACCTTTGA TTAAAACCAT GGGTGATGGT ACCTTGGGAG GATTTGACAT GGAGAATTTG
    TTGCAATCTT TGTATGTAGA AAACAGAGCT GGATTCTTCT TTACTAAATT CTGCGAGAAC
    TCAGGGAACA AGTTGTGGAA GAACTGTGTG TACTTTTGGT TTGACCTACA AGCTTATCAT
    CAGCTTTTCT ACCAAGAAAC CCTTCACCCT TTTAAAGTAT GCAAGCAAGC TCAATATCTT
    TTTGCTACAT ACATTGCTCC TTCAGCCACT CTTGACATTG GACTTCAACA GGAAAAGAAA
    AGAGAAATTT ATATGAAAAT ACAACCACCA TTTGAAGACC TTTTTGATGC AGCAGAAGAA
    TATATCCTCC TCCTCCTTCT TGAGCCTTGG ACAAAGATGA TAAAATCAGA CCAAGTTGCT
    TACAGAAAGG TGGAGCTGGT GGAAGAAACT CGACAGCTGG ATTCCACATA CTTGAGAAAG
    CTACAGGCTT TGCATAAAGA GACAGTTTTC AAGAAAGCTG AGGACACTAC TTCTGAAATT
    GGAACTGGTA TTTCACCACT TCCTCATGTC TCTAAAAACA CAGAATACTG GAATATGGTT
    CCTGAAGAAT ATAGGCATTT TAATTTTAAT GAACTGCTTA ACAACAAACT GGAGTTTGAA
    CATTTCCGTC AGTTTCTTGA GGCTCATTCT TCAAGCATGG ACCTTATGTG CTGGACAGAC
    ATTGAACAGT TCCGAAGAAT AACTTACAGA GATCGAAAGC AAAGGGAGGC AAAATCTATA
    TATATAAAAA ACAAATACCT CAATAAAAAG TATTTCTTTG GGCCCAACAG TCCAGCTTCT
    GTGTATCAAC AGGACCAGAT AATGAATTTA AGTGGTGGCT GGGGAAAGAT TCTCCATGAG
    CAACTTGATG CAGCTGTTCT AATCGAAATT CAGAAGCATG TCCTAAATAG ACTAGAAAAT
    GTTTGGTTGC CGTTGTTTCT TGCAAGTGAA CAGTTTGCGG CACGTCAAAA GATAAAGGTA
    CAGATAAAAG ACATAGCTGA AGAGCTTTTA CTACAAAAGC GTGAAAGGAA AATTGGAGTC
    TGGAAGCCTG TGGAGAGTAA GTGGATATCT TCGTCTTGTG AAATCATTGC TTTCCGTAAA
    GCTTTATTGA ATCCAGTTAC TGCAAGACAA TTTCAACGCT TTGTGGCTCT AAAAGGAGAT
    TTATTGGAAA ATGGGGTGCT CTTTTGGCAA GAAGTACAAA AATATAAGGA CTTGTGCCAT
    TCTCATTGTG ATGAGTCTAT CATACACAAG AAGATTATGA CTATTATCAA CTGCTTTATT
    AATTCCAGCA TCCCCCCAGC TTTACAAATT GACATTCCAG TAGAGCAAGC CCAGAAGATT
    ATTGAACACA GGAAGGAGTT GGGACCATAT GTATTCAGAG AGGCACAGAT GACAATTTTT
    GGAGTTCTGT TTAAATTTTG GCCTCAGTTC TGTGAGTTTA GGAAGAACTT AACTGATGAA
    AAAATTATGA GTGTTTTGGA GAGAAGACAA GAATATAGTA AGCAGAAAAA GAAAATGGCA
    GTCATAGAAG ATGAAAAATT TGTAAAGGAT GGAATCAAAC AATATTCGAG TACTTCAGGA
    TCTGTTATCA AAGCAGCTGC TTTAACTAGT GACCCAGGTT TAGGCATGCA AGCGTACGGC
    CGACAGCCAA CCTGGTGCTA CTCAAAGTAC ATAGAAGCCT TAGAACAGGA AAGGATTCTT
    CTTAAAATTC AAGAGGAACT AGAAAGAAAG TTGTTTGCAG CCACACCATC TTTTACAAAT
    TTCAAGTCTA CTGCTTCCAC TCTGTCTTTG AAAAAGACTG TGTCCTCCCA CAACATCCAG
    AGAATTCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92564
    Clone ID Related Accession (Same CDS sequence) XM_015466376.1 , XM_015474518.1 , XM_015474518.2
    Accession Version XM_015474518.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulator of G-protein signaling 22 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474518.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCATGT
    CTCAATCGTG AACAAGGTAT TCACTGGGTC AAAAAAGAAA GACTTCCAAC TTTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGGTTAGCC AAATTGATTT CACAAGTAAG ATGGAGTGAA
    TCAGGCATGA ATATCATATT AGGTACAGAG TTTTCACCCT GGGTCCTGAA AAAACCACCC
    AGTCCACCAC CTCCTCCTGT TGAAGAAGAT GATGTTATAA TTATGAAGAA GTTCTACATT
    TCTCTGGGCG AGGCCTCCTA TACTCAAACA AAAGATTGGT TTGCCTTAGC AAAACAATGT
    CAGCAAACAG TACCAACCTT TTCACTACCA TCTTGTGTAT TCCACAACAA AATTGAATCA
    CCAGTTGTTT CATCTGCTTC TGAGAGTTTT ATCTTCGATG ATGGAGTTCA CCCCAGGACG
    AAAAAGGGCC TATTAAAAAC CACTAGATTA ACCTCCGAAT TTGAAGAAGA TGGGTCTGTA
    TCTCTACAGG ACACTCCTTC TCAAGCTCTT CTGAGGATAT ACCTTGAAAA GCAACAGAAT
    GTTGATGGAA GTCTGACACT GCACTTCTCA ACATGTGACG AGTTTTTAAG ATCTTATGTC
    TCCTTTATCT TGAGAGTGGC GATTCATCAA ATTCTTGGAG CAACACTTAG AGAAAGCCCA
    GATTACGTAA ATTTCAGAAA CGTAACAGAA GTGGTATTTG ATGACATTGG GGGGCCTACC
    TCTGGCAAGA ATGTTTTGTT CAATGAAATG CTAAGGGAAG AGTCGGGAGA CATGTTAGAA
    CCAGCAGTTT CAAAGCCTGA CAGCATTGGA CTCGACAGCA GGGCTGATTG GTGTATTTCT
    CACAAAACTT ATGACATTGG TAATAGAAAG GAGTTTGAAA GATTTAAGAA ATTTTTAAAA
    GGTACCTTAG GGGAGAAATA TTGGTGGCTA TGGATGGATA TTGAGAGGTT AAAAGTTCTT
    AAGAATCCTG GAAGACATCA AAGACATATT GAGAAGATGA AAAAATGCTT TCTAGTGAGC
    AGTGGAGATT ACTACCTTTC TGCAGAAGTC TTATCAAAAT TTAAACTACT AGATGGATCT
    CAGTGGAATG AAGAACACTT AAAAAAAATT CAGACTGAGG TTTTAAAGCC CTTACTTCTT
    TATTGGGCAC CAAGATTCTG TGTTACTCAC TCATCCTCAA CAAAACATGC TAGTGCCGAG
    CTGAAATTCT GGCACCTTCG TCAGGAGCAG CCGAGGAAGG ATGTTGACCC TTTCCCACAA
    ATGGCAACCC TCTTGCCGTT AAGACCCAAA TCTTGCATTC CACAGCTACC TGAGATGCAG
    AAAGAAGAAT CCAGTTTACT ACAACTTCCT AAATCTCCCA AGAAATCACC TAGAGTAAAG
    ACAGCAGTTT ATAAACCTTG GAAGAGTGAG TCTTTGAATC CAGTTTCTTC TAAGGATGAT
    GTGATTGAGA AAGGGTCAAG GCACATGTCA GAAAGCAGCA AAGTTATTCG TTTAACATCT
    TTTACTGACA TTTCTGAATG TCTGAAGCCC CAGCTGGATA GAAGATATAC CTATACAGAA
    GAACCTTTGA TTAAAACCAT GGGTGATGGT ACCTTGGGAG GATTTGACAT GGAGAATTTG
    TTGCAATCTT TGTATGTAGA AAACAGAGCT GGATTCTTCT TTACTAAATT CTGCGAGAAC
    TCAGGGAACA AGTTGTGGAA GAACTGTGTG TACTTTTGGT TTGACCTACA AGCTTATCAT
    CAGCTTTTCT ACCAAGAAAC CCTTCACCCT TTTAAAGTAT GCAAGCAAGC TCAATATCTT
    TTTGCTACAT ACATTGCTCC TTCAGCCACT CTTGACATTG GACTTCAACA GGAAAAGAAA
    AGAGAAATTT ATATGAAAAT ACAACCACCA TTTGAAGACC TTTTTGATGC AGCAGAAGAA
    TATATCCTCC TCCTCCTTCT TGAGCCTTGG ACAAAGATGA TAAAATCAGA CCAAGTTGCT
    TACAGAAAGG TGGAGCTGGT GGAAGAAACT CGACAGCTGG ATTCCACATA CTTGAGAAAG
    CTACAGGCTT TGCATAAAGA GACAGTTTTC AAGAAAGCTG AGGACACTAC TTCTGAAATT
    GGAACTGGTA TTTCACCACT TCCTCATGTC TCTAAAAACA CAGAATACTG GAATATGGTT
    CCTGAAGAAT ATAGGCATTT TAATTTTAAT GAACTGCTTA ACAACAAACT GGAGTTTGAA
    CATTTCCGTC AGTTTCTTGA GGCTCATTCT TCAAGCATGG ACCTTATGTG CTGGACAGAC
    ATTGAACAGT TCCGAAGAAT AACTTACAGA GATCGAAAGC AAAGGGAGGC AAAATCTATA
    TATATAAAAA ACAAATACCT CAATAAAAAG TATTTCTTTG GGCCCAACAG TCCAGCTTCT
    GTGTATCAAC AGGACCAGAT AATGAATTTA AGTGGTGGCT GGGGAAAGAT TCTCCATGAG
    CAACTTGATG CAGCTGTTCT AATCGAAATT CAGAAGCATG TCCTAAATAG ACTAGAAAAT
    GTTTGGTTGC CGTTGTTTCT TGCAAGTGAA CAGTTTGCGG CACGTCAAAA GATAAAGGTA
    CAGATAAAAG ACATAGCTGA AGAGCTTTTA CTACAAAAGC GTGAAAGGAA AATTGGAGTC
    TGGAAGCCTG TGGAGAGTAA GTGGATATCT TCGTCTTGTG AAATCATTGC TTTCCGTAAA
    GCTTTATTGA ATCCAGTTAC TGCAAGACAA TTTCAACGCT TTGTGGCTCT AAAAGGAGAT
    TTATTGGAAA ATGGGGTGCT CTTTTGGCAA GAAGTACAAA AATATAAGGA CTTGTGCCAT
    TCTCATTGTG ATGAGTCTAT CATACACAAG AAGATTATGA CTATTATCAA CTGCTTTATT
    AATTCCAGCA TCCCCCCAGC TTTACAAATT GACATTCCAG TAGAGCAAGC CCAGAAGATT
    ATTGAACACA GGAAGGAGTT GGGACCATAT GTATTCAGAG AGGCACAGAT GACAATTTTT
    GGAGTTCTGT TTAAATTTTG GCCTCAGTTC TGTGAGTTTA GGAAGAACTT AACTGATGAA
    AAAATTATGA GTGTTTTGGA GAGAAGACAA GAATATAGTA AGCAGAAAAA GAAAATGGCA
    GTCATAGAAG ATGAAAAATT TGTAAAGGAT GGAATCAAAC AATATTCGAG TACTTCAGGA
    TCTGTTATCA AAGCAGCTGC TTTAACTAGT GACCCAGGTT TAGGCATGCA AGCGTACGGC
    CGACAGCCAA CCTGGTGCTA CTCAAAGTAC ATAGAAGCCT TAGAACAGGA AAGGATTCTT
    CTTAAAATTC AAGAGGAACT AGAAAGAAAG TTGTTTGCAG CCACACCATC TTTTACAAAT
    TTCAAGTCTA CTGCTTCCAC TCTGTCTTTG AAAAAGACTG TGTCCTCCCA CAACATCCAG
    AGAATTCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330004.1
    CDS77..3628
    Translation

    Target ORF information:

    RefSeq Version XM_015474518.2
    Organism Bos taurus(cattle)
    Definition Bos taurus regulator of G protein signaling 22 (RGS22), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474518.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCATGT
    CTCAATCGTG AACAAGGTAT TCACTGGGTC AAAAAAGAAA GACTTCCAAC TTTTCTGGAA
    AGTGATTGTT ACTTTGAATA CAGGTTAGCC AAATTGATTT CACAAGTAAG ATGGAGTGAA
    TCAGGCATGA ATATCATATT AGGTACAGAG TTTTCACCCT GGGTCCTGAA AAAACCACCC
    AGTCCACCAC CTCCTCCTGT TGAAGAAGAT GATGTTATAA TTATGAAGAA GTTCTACATT
    TCTCTGGGCG AGGCCTCCTA TACTCAAACA AAAGATTGGT TTGCCTTAGC AAAACAATGT
    CAGCAAACAG TACCAACCTT TTCACTACCA TCTTGTGTAT TCCACAACAA AATTGAATCA
    CCAGTTGTTT CATCTGCTTC TGAGAGTTTT ATCTTCGATG ATGGAGTTCA CCCCAGGACG
    AAAAAGGGCC TATTAAAAAC CACTAGATTA ACCTCCGAAT TTGAAGAAGA TGGGTCTGTA
    TCTCTACAGG ACACTCCTTC TCAAGCTCTT CTGAGGATAT ACCTTGAAAA GCAACAGAAT
    GTTGATGGAA GTCTGACACT GCACTTCTCA ACATGTGACG AGTTTTTAAG ATCTTATGTC
    TCCTTTATCT TGAGAGTGGC GATTCATCAA ATTCTTGGAG CAACACTTAG AGAAAGCCCA
    GATTACGTAA ATTTCAGAAA CGTAACAGAA GTGGTATTTG ATGACATTGG GGGGCCTACC
    TCTGGCAAGA ATGTTTTGTT CAATGAAATG CTAAGGGAAG AGTCGGGAGA CATGTTAGAA
    CCAGCAGTTT CAAAGCCTGA CAGCATTGGA CTCGACAGCA GGGCTGATTG GTGTATTTCT
    CACAAAACTT ATGACATTGG TAATAGAAAG GAGTTTGAAA GATTTAAGAA ATTTTTAAAA
    GGTACCTTAG GGGAGAAATA TTGGTGGCTA TGGATGGATA TTGAGAGGTT AAAAGTTCTT
    AAGAATCCTG GAAGACATCA AAGACATATT GAGAAGATGA AAAAATGCTT TCTAGTGAGC
    AGTGGAGATT ACTACCTTTC TGCAGAAGTC TTATCAAAAT TTAAACTACT AGATGGATCT
    CAGTGGAATG AAGAACACTT AAAAAAAATT CAGACTGAGG TTTTAAAGCC CTTACTTCTT
    TATTGGGCAC CAAGATTCTG TGTTACTCAC TCATCCTCAA CAAAACATGC TAGTGCCGAG
    CTGAAATTCT GGCACCTTCG TCAGGAGCAG CCGAGGAAGG ATGTTGACCC TTTCCCACAA
    ATGGCAACCC TCTTGCCGTT AAGACCCAAA TCTTGCATTC CACAGCTACC TGAGATGCAG
    AAAGAAGAAT CCAGTTTACT ACAACTTCCT AAATCTCCCA AGAAATCACC TAGAGTAAAG
    ACAGCAGTTT ATAAACCTTG GAAGAGTGAG TCTTTGAATC CAGTTTCTTC TAAGGATGAT
    GTGATTGAGA AAGGGTCAAG GCACATGTCA GAAAGCAGCA AAGTTATTCG TTTAACATCT
    TTTACTGACA TTTCTGAATG TCTGAAGCCC CAGCTGGATA GAAGATATAC CTATACAGAA
    GAACCTTTGA TTAAAACCAT GGGTGATGGT ACCTTGGGAG GATTTGACAT GGAGAATTTG
    TTGCAATCTT TGTATGTAGA AAACAGAGCT GGATTCTTCT TTACTAAATT CTGCGAGAAC
    TCAGGGAACA AGTTGTGGAA GAACTGTGTG TACTTTTGGT TTGACCTACA AGCTTATCAT
    CAGCTTTTCT ACCAAGAAAC CCTTCACCCT TTTAAAGTAT GCAAGCAAGC TCAATATCTT
    TTTGCTACAT ACATTGCTCC TTCAGCCACT CTTGACATTG GACTTCAACA GGAAAAGAAA
    AGAGAAATTT ATATGAAAAT ACAACCACCA TTTGAAGACC TTTTTGATGC AGCAGAAGAA
    TATATCCTCC TCCTCCTTCT TGAGCCTTGG ACAAAGATGA TAAAATCAGA CCAAGTTGCT
    TACAGAAAGG TGGAGCTGGT GGAAGAAACT CGACAGCTGG ATTCCACATA CTTGAGAAAG
    CTACAGGCTT TGCATAAAGA GACAGTTTTC AAGAAAGCTG AGGACACTAC TTCTGAAATT
    GGAACTGGTA TTTCACCACT TCCTCATGTC TCTAAAAACA CAGAATACTG GAATATGGTT
    CCTGAAGAAT ATAGGCATTT TAATTTTAAT GAACTGCTTA ACAACAAACT GGAGTTTGAA
    CATTTCCGTC AGTTTCTTGA GGCTCATTCT TCAAGCATGG ACCTTATGTG CTGGACAGAC
    ATTGAACAGT TCCGAAGAAT AACTTACAGA GATCGAAAGC AAAGGGAGGC AAAATCTATA
    TATATAAAAA ACAAATACCT CAATAAAAAG TATTTCTTTG GGCCCAACAG TCCAGCTTCT
    GTGTATCAAC AGGACCAGAT AATGAATTTA AGTGGTGGCT GGGGAAAGAT TCTCCATGAG
    CAACTTGATG CAGCTGTTCT AATCGAAATT CAGAAGCATG TCCTAAATAG ACTAGAAAAT
    GTTTGGTTGC CGTTGTTTCT TGCAAGTGAA CAGTTTGCGG CACGTCAAAA GATAAAGGTA
    CAGATAAAAG ACATAGCTGA AGAGCTTTTA CTACAAAAGC GTGAAAGGAA AATTGGAGTC
    TGGAAGCCTG TGGAGAGTAA GTGGATATCT TCGTCTTGTG AAATCATTGC TTTCCGTAAA
    GCTTTATTGA ATCCAGTTAC TGCAAGACAA TTTCAACGCT TTGTGGCTCT AAAAGGAGAT
    TTATTGGAAA ATGGGGTGCT CTTTTGGCAA GAAGTACAAA AATATAAGGA CTTGTGCCAT
    TCTCATTGTG ATGAGTCTAT CATACACAAG AAGATTATGA CTATTATCAA CTGCTTTATT
    AATTCCAGCA TCCCCCCAGC TTTACAAATT GACATTCCAG TAGAGCAAGC CCAGAAGATT
    ATTGAACACA GGAAGGAGTT GGGACCATAT GTATTCAGAG AGGCACAGAT GACAATTTTT
    GGAGTTCTGT TTAAATTTTG GCCTCAGTTC TGTGAGTTTA GGAAGAACTT AACTGATGAA
    AAAATTATGA GTGTTTTGGA GAGAAGACAA GAATATAGTA AGCAGAAAAA GAAAATGGCA
    GTCATAGAAG ATGAAAAATT TGTAAAGGAT GGAATCAAAC AATATTCGAG TACTTCAGGA
    TCTGTTATCA AAGCAGCTGC TTTAACTAGT GACCCAGGTT TAGGCATGCA AGCGTACGGC
    CGACAGCCAA CCTGGTGCTA CTCAAAGTAC ATAGAAGCCT TAGAACAGGA AAGGATTCTT
    CTTAAAATTC AAGAGGAACT AGAAAGAAAG TTGTTTGCAG CCACACCATC TTTTACAAAT
    TTCAAGTCTA CTGCTTCCAC TCTGTCTTTG AAAAAGACTG TGTCCTCCCA CAACATCCAG
    AGAATTCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07980
    Clone ID Related Accession (Same CDS sequence) XM_015466375.1 , XM_005215633.3 , XM_005215633.4
    Accession Version XM_015466375.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulator of G-protein signaling 22 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646230.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGTC TATGGAGTTT TTGAAGTAGT TAACAATGCT
    CCACAACTTC TGGAAAAGCA GTTGAAAAAA ATTGTACTAA ACCAGCGACC TCGAAATCCT
    ATCTATGATG TTGTAAGGAA AGGAAAGAGT GATGTTAAAC CTGTTCAAGT GAACGCCCCT
    CACGAAGATG AGACCATTAA TGTCAACTAC AATATCATGT GTCTCAATCG TGAACAAGGT
    ATTCACTGGG TCAAAAAAGA AAGACTTCCA ACTTTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGGTTAG CCAAATTGAT TTCACAAGTA AGATGGAGTG AATCAGGCAT GAATATCATA
    TTAGGTACAG AGTTTTCACC CTGGGTCCTG AAAAAACCAC CCAGTCCACC ACCTCCTCCT
    GTTGAAGAAG ATGATGTTAT AATTATGAAG AAGTTCTACA TTTCTCTGGG CGAGGCCTCC
    TATACTCAAA CAAAAGATTG GTTTGCCTTA GCAAAACAAT GTCAGCAAAC AGTACCAACC
    TTTTCACTAC CATCTTGTGT ATTCCACAAC AAAATTGAAT CACCAGTTGT TTCATCTGCT
    TCTGAGAGTT TTATCTTCGA TGATGGAGTT CACCCCAGGA CGAAAAAGGG CCTATTAAAA
    ACCACTAGAT TAACCTCCGA ATTTGAAGAA GATGGGTCTG TATCTCTACA GGACACTCCT
    TCTCAAGCTC TTCTGAGGAT ATACCTTGAA AAGCAACAGA ATGTTGATGG AAGTCTGACA
    CTGCACTTCT CAACATGTGA CGAGTTTTTA AGATCTTATG TCTCCTTTAT CTTGAGAGTG
    GCGATTCATC AAATTCTTGG AGCAACACTT AGAGAAAGCC CAGATTACGT AAATTTCAGA
    AACGTAACAG AAGTGGTATT TGATGACATT GGGGGGCCTA CCTCTGGCAA GAATGTTTTG
    TTCAATGAAA TGCTAAGGGA AGAGTCGGGA GACATGTTAG AACCAGCAGT TTCAAAGCCT
    GACAGCATTG GACTCGACAG CAGGGCTGAT TGGTGTATTT CTCACAAAAC TTATGACATT
    GGTAATAGAA AGGAGTTTGA AAGATTTAAG AAATTTTTAA AAGGTACCTT AGGGGAGAAA
    TATTGGTGGC TATGGATGGA TATTGAGAGG TTAAAAGTTC TTAAGAATCC TGGAAGACAT
    CAAAGACATA TTGAGAAGAT GAAAAAATGC TTTCTAGTGA GCAGTGGAGA TTACTACCTT
    TCTGCAGAAG TCTTATCAAA ATTTAAACTA CTAGATGGAT CTCAGTGGAA TGAAGAACAC
    TTAAAAAAAA TTCAGACTGA GGTTTTAAAG CCCTTACTTC TTTATTGGGC ACCAAGATTC
    TGTGTTACTC ACTCATCCTC AACAAAACAT GCTAGTGCCG AGCTGAAATT CTGGCACCTT
    CGTCAGGAGC AGCCGAGGAA GGATGTTGAC CCTTTCCCAC AAATGGCAAC CCTCTTGCCG
    TTAAGACCCA AATCTTGCAT TCCACAGCTA CCTGAGATGC AGAAAGAAGA ATCCAGTTTA
    CTACAACTTC CTAAATCTCC CAAGAAATCA CCTAGAGTAA AGACAGCAGT TTATAAACCT
    TGGAAGAGTG AGTCTTTGAA TCCAGTTTCT TCTAAGGATG ATGTGATTGA GAAAGGGTCA
    AGGCACATGT CAGAAAGCAG CAAAGTTATT CGTTTAACAT CTTTTACTGA CATTTCTGAA
    TGTCTGAAGC CCCAGCTGGA TAGAAGATAT ACCTATACAG AAGAACCTTT GATTAAAACC
    ATGGGTGATG GTACCTTGGG AGGATTTGAC ATGGAGAATT TGTTGCAATC TTTGTATGTA
    GAAAACAGAG CTGGATTCTT CTTTACTAAA TTCTGCGAGA ACTCAGGGAA CAAGTTGTGG
    AAGAACTGTG TGTACTTTTG GTTTGACCTA CAAGCTTATC ATCAGCTTTT CTACCAAGAA
    ACCCTTCACC CTTTTAAAGT ATGCAAGCAA GCTCAATATC TTTTTGCTAC ATACATTGCT
    CCTTCAGCCA CTCTTGACAT TGGACTTCAA CAGGAAAAGA AAAGAGAAAT TTATATGAAA
    ATACAACCAC CATTTGAAGA CCTTTTTGAT GCAGCAGAAG AATATATCCT CCTCCTCCTT
    CTTGAGCCTT GGACAAAGAT GATAAAATCA GACCAAGTTG CTTACAGAAA GGTGGAGCTG
    GTGGAAGAAA CTCGACAGCT GGATTCCACA TACTTGAGAA AGCTACAGGC TTTGCATAAA
    GAGACAGTTT TCAAGAAAGC TGAGGACACT ACTTCTGAAA TTGGAACTGG TATTTCACCA
    CTTCCTCATG TCTCTAAAAA CACAGAATAC TGGAATATGG TTCCTGAAGA ATATAGGCAT
    TTTAATTTTA ATGAACTGCT TAACAACAAA CTGGAGTTTG AACATTTCCG TCAGTTTCTT
    GAGGCTCATT CTTCAAGCAT GGACCTTATG TGCTGGACAG ACATTGAACA GTTCCGAAGA
    ATAACTTACA GAGATCGAAA GCAAAGGGAG GCAAAATCTA TATATATAAA AAACAAATAC
    CTCAATAAAA AGTATTTCTT TGGGCCCAAC AGTCCAGCTT CTGTGTATCA ACAGGACCAG
    ATAATGAATT TAAGTGGTGG CTGGGGAAAG ATTCTCCATG AGCAACTTGA TGCAGCTGTT
    CTAATCGAAA TTCAGAAGCA TGTCCTAAAT AGACTAGAAA ATGTTTGGTT GCCGTTGTTT
    CTTGCAAGTG AACAGTTTGC GGCACGTCAA AAGATAAAGG TACAGATAAA AGACATAGCT
    GAAGAGCTTT TACTACAAAA GCGTGAAAGG AAAATTGGAG TCTGGAAGCC TGTGGAGAGT
    AAGTGGATAT CTTCGTCTTG TGAAATCATT GCTTTCCGTA AAGCTTTATT GAATCCAGTT
    ACTGCAAGAC AATTTCAACG CTTTGTGGCT CTAAAAGGAG ATTTATTGGA AAATGGGGTG
    CTCTTTTGGC AAGAAGTACA AAAATATAAG GACTTGTGCC ATTCTCATTG TGATGAGTCT
    ATCATACACA AGAAGATTAT GACTATTATC AACTGCTTTA TTAATTCCAG CATCCCCCCA
    GCTTTACAAA TTGACATTCC AGTAGAGCAA GCCCAGAAGA TTATTGAACA CAGGAAGGAG
    TTGGGACCAT ATGTATTCAG AGAGGCACAG ATGACAATTT TTGGAGTTCT GTTTAAATTT
    TGGCCTCAGT TCTGTGAGTT TAGGAAGAAC TTAACTGATG AAAAAATTAT GAGTGTTTTG
    GAGAGAAGAC AAGAATATAG TAAGCAGAAA AAGAAAATGG CAGTCATAGA AGATGAAAAA
    TTTGTAAAGG ATGGAATCAA ACAATATTCG AGTACTTCAG GATCTGTTAT CAAAGCAGCT
    GCTTTAACTA GTGACCCAGG TTTAGGCATG CAAGCGTACG GCCGACAGCC AACCTGGTGC
    TACTCAAAGT ACATAGAAGC CTTAGAACAG GAAAGGATTC TTCTTAAAAT TCAAGAGGAA
    CTAGAAAGAA AGTTGTTTGC AGGAATTCTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321861.1
    CDS47..3739
    Translation

    Target ORF information:

    RefSeq Version XM_015466375.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulator of G-protein signaling 22 (RGS22), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466375.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGTC TATGGAGTTT TTGAAGTAGT TAACAATGCT
    CCACAACTTC TGGAAAAGCA GTTGAAAAAA ATTGTACTAA ACCAGCGACC TCGAAATCCT
    ATCTATGATG TTGTAAGGAA AGGAAAGAGT GATGTTAAAC CTGTTCAAGT GAACGCCCCT
    CACGAAGATG AGACCATTAA TGTCAACTAC AATATCATGT GTCTCAATCG TGAACAAGGT
    ATTCACTGGG TCAAAAAAGA AAGACTTCCA ACTTTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGGTTAG CCAAATTGAT TTCACAAGTA AGATGGAGTG AATCAGGCAT GAATATCATA
    TTAGGTACAG AGTTTTCACC CTGGGTCCTG AAAAAACCAC CCAGTCCACC ACCTCCTCCT
    GTTGAAGAAG ATGATGTTAT AATTATGAAG AAGTTCTACA TTTCTCTGGG CGAGGCCTCC
    TATACTCAAA CAAAAGATTG GTTTGCCTTA GCAAAACAAT GTCAGCAAAC AGTACCAACC
    TTTTCACTAC CATCTTGTGT ATTCCACAAC AAAATTGAAT CACCAGTTGT TTCATCTGCT
    TCTGAGAGTT TTATCTTCGA TGATGGAGTT CACCCCAGGA CGAAAAAGGG CCTATTAAAA
    ACCACTAGAT TAACCTCCGA ATTTGAAGAA GATGGGTCTG TATCTCTACA GGACACTCCT
    TCTCAAGCTC TTCTGAGGAT ATACCTTGAA AAGCAACAGA ATGTTGATGG AAGTCTGACA
    CTGCACTTCT CAACATGTGA CGAGTTTTTA AGATCTTATG TCTCCTTTAT CTTGAGAGTG
    GCGATTCATC AAATTCTTGG AGCAACACTT AGAGAAAGCC CAGATTACGT AAATTTCAGA
    AACGTAACAG AAGTGGTATT TGATGACATT GGGGGGCCTA CCTCTGGCAA GAATGTTTTG
    TTCAATGAAA TGCTAAGGGA AGAGTCGGGA GACATGTTAG AACCAGCAGT TTCAAAGCCT
    GACAGCATTG GACTCGACAG CAGGGCTGAT TGGTGTATTT CTCACAAAAC TTATGACATT
    GGTAATAGAA AGGAGTTTGA AAGATTTAAG AAATTTTTAA AAGGTACCTT AGGGGAGAAA
    TATTGGTGGC TATGGATGGA TATTGAGAGG TTAAAAGTTC TTAAGAATCC TGGAAGACAT
    CAAAGACATA TTGAGAAGAT GAAAAAATGC TTTCTAGTGA GCAGTGGAGA TTACTACCTT
    TCTGCAGAAG TCTTATCAAA ATTTAAACTA CTAGATGGAT CTCAGTGGAA TGAAGAACAC
    TTAAAAAAAA TTCAGACTGA GGTTTTAAAG CCCTTACTTC TTTATTGGGC ACCAAGATTC
    TGTGTTACTC ACTCATCCTC AACAAAACAT GCTAGTGCCG AGCTGAAATT CTGGCACCTT
    CGTCAGGAGC AGCCGAGGAA GGATGTTGAC CCTTTCCCAC AAATGGCAAC CCTCTTGCCG
    TTAAGACCCA AATCTTGCAT TCCACAGCTA CCTGAGATGC AGAAAGAAGA ATCCAGTTTA
    CTACAACTTC CTAAATCTCC CAAGAAATCA CCTAGAGTAA AGACAGCAGT TTATAAACCT
    TGGAAGAGTG AGTCTTTGAA TCCAGTTTCT TCTAAGGATG ATGTGATTGA GAAAGGGTCA
    AGGCACATGT CAGAAAGCAG CAAAGTTATT CGTTTAACAT CTTTTACTGA CATTTCTGAA
    TGTCTGAAGC CCCAGCTGGA TAGAAGATAT ACCTATACAG AAGAACCTTT GATTAAAACC
    ATGGGTGATG GTACCTTGGG AGGATTTGAC ATGGAGAATT TGTTGCAATC TTTGTATGTA
    GAAAACAGAG CTGGATTCTT CTTTACTAAA TTCTGCGAGA ACTCAGGGAA CAAGTTGTGG
    AAGAACTGTG TGTACTTTTG GTTTGACCTA CAAGCTTATC ATCAGCTTTT CTACCAAGAA
    ACCCTTCACC CTTTTAAAGT ATGCAAGCAA GCTCAATATC TTTTTGCTAC ATACATTGCT
    CCTTCAGCCA CTCTTGACAT TGGACTTCAA CAGGAAAAGA AAAGAGAAAT TTATATGAAA
    ATACAACCAC CATTTGAAGA CCTTTTTGAT GCAGCAGAAG AATATATCCT CCTCCTCCTT
    CTTGAGCCTT GGACAAAGAT GATAAAATCA GACCAAGTTG CTTACAGAAA GGTGGAGCTG
    GTGGAAGAAA CTCGACAGCT GGATTCCACA TACTTGAGAA AGCTACAGGC TTTGCATAAA
    GAGACAGTTT TCAAGAAAGC TGAGGACACT ACTTCTGAAA TTGGAACTGG TATTTCACCA
    CTTCCTCATG TCTCTAAAAA CACAGAATAC TGGAATATGG TTCCTGAAGA ATATAGGCAT
    TTTAATTTTA ATGAACTGCT TAACAACAAA CTGGAGTTTG AACATTTCCG TCAGTTTCTT
    GAGGCTCATT CTTCAAGCAT GGACCTTATG TGCTGGACAG ACATTGAACA GTTCCGAAGA
    ATAACTTACA GAGATCGAAA GCAAAGGGAG GCAAAATCTA TATATATAAA AAACAAATAC
    CTCAATAAAA AGTATTTCTT TGGGCCCAAC AGTCCAGCTT CTGTGTATCA ACAGGACCAG
    ATAATGAATT TAAGTGGTGG CTGGGGAAAG ATTCTCCATG AGCAACTTGA TGCAGCTGTT
    CTAATCGAAA TTCAGAAGCA TGTCCTAAAT AGACTAGAAA ATGTTTGGTT GCCGTTGTTT
    CTTGCAAGTG AACAGTTTGC GGCACGTCAA AAGATAAAGG TACAGATAAA AGACATAGCT
    GAAGAGCTTT TACTACAAAA GCGTGAAAGG AAAATTGGAG TCTGGAAGCC TGTGGAGAGT
    AAGTGGATAT CTTCGTCTTG TGAAATCATT GCTTTCCGTA AAGCTTTATT GAATCCAGTT
    ACTGCAAGAC AATTTCAACG CTTTGTGGCT CTAAAAGGAG ATTTATTGGA AAATGGGGTG
    CTCTTTTGGC AAGAAGTACA AAAATATAAG GACTTGTGCC ATTCTCATTG TGATGAGTCT
    ATCATACACA AGAAGATTAT GACTATTATC AACTGCTTTA TTAATTCCAG CATCCCCCCA
    GCTTTACAAA TTGACATTCC AGTAGAGCAA GCCCAGAAGA TTATTGAACA CAGGAAGGAG
    TTGGGACCAT ATGTATTCAG AGAGGCACAG ATGACAATTT TTGGAGTTCT GTTTAAATTT
    TGGCCTCAGT TCTGTGAGTT TAGGAAGAAC TTAACTGATG AAAAAATTAT GAGTGTTTTG
    GAGAGAAGAC AAGAATATAG TAAGCAGAAA AAGAAAATGG CAGTCATAGA AGATGAAAAA
    TTTGTAAAGG ATGGAATCAA ACAATATTCG AGTACTTCAG GATCTGTTAT CAAAGCAGCT
    GCTTTAACTA GTGACCCAGG TTTAGGCATG CAAGCGTACG GCCGACAGCC AACCTGGTGC
    TACTCAAAGT ACATAGAAGC CTTAGAACAG GAAAGGATTC TTCTTAAAAT TCAAGAGGAA
    CTAGAAAGAA AGTTGTTTGC AGGAATTCTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07980
    Clone ID Related Accession (Same CDS sequence) XM_015466375.1 , XM_005215633.3 , XM_005215633.4
    Accession Version XM_005215633.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulator of G-protein signaling 22 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104399.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215633.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGTC TATGGAGTTT TTGAAGTAGT TAACAATGCT
    CCACAACTTC TGGAAAAGCA GTTGAAAAAA ATTGTACTAA ACCAGCGACC TCGAAATCCT
    ATCTATGATG TTGTAAGGAA AGGAAAGAGT GATGTTAAAC CTGTTCAAGT GAACGCCCCT
    CACGAAGATG AGACCATTAA TGTCAACTAC AATATCATGT GTCTCAATCG TGAACAAGGT
    ATTCACTGGG TCAAAAAAGA AAGACTTCCA ACTTTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGGTTAG CCAAATTGAT TTCACAAGTA AGATGGAGTG AATCAGGCAT GAATATCATA
    TTAGGTACAG AGTTTTCACC CTGGGTCCTG AAAAAACCAC CCAGTCCACC ACCTCCTCCT
    GTTGAAGAAG ATGATGTTAT AATTATGAAG AAGTTCTACA TTTCTCTGGG CGAGGCCTCC
    TATACTCAAA CAAAAGATTG GTTTGCCTTA GCAAAACAAT GTCAGCAAAC AGTACCAACC
    TTTTCACTAC CATCTTGTGT ATTCCACAAC AAAATTGAAT CACCAGTTGT TTCATCTGCT
    TCTGAGAGTT TTATCTTCGA TGATGGAGTT CACCCCAGGA CGAAAAAGGG CCTATTAAAA
    ACCACTAGAT TAACCTCCGA ATTTGAAGAA GATGGGTCTG TATCTCTACA GGACACTCCT
    TCTCAAGCTC TTCTGAGGAT ATACCTTGAA AAGCAACAGA ATGTTGATGG AAGTCTGACA
    CTGCACTTCT CAACATGTGA CGAGTTTTTA AGATCTTATG TCTCCTTTAT CTTGAGAGTG
    GCGATTCATC AAATTCTTGG AGCAACACTT AGAGAAAGCC CAGATTACGT AAATTTCAGA
    AACGTAACAG AAGTGGTATT TGATGACATT GGGGGGCCTA CCTCTGGCAA GAATGTTTTG
    TTCAATGAAA TGCTAAGGGA AGAGTCGGGA GACATGTTAG AACCAGCAGT TTCAAAGCCT
    GACAGCATTG GACTCGACAG CAGGGCTGAT TGGTGTATTT CTCACAAAAC TTATGACATT
    GGTAATAGAA AGGAGTTTGA AAGATTTAAG AAATTTTTAA AAGGTACCTT AGGGGAGAAA
    TATTGGTGGC TATGGATGGA TATTGAGAGG TTAAAAGTTC TTAAGAATCC TGGAAGACAT
    CAAAGACATA TTGAGAAGAT GAAAAAATGC TTTCTAGTGA GCAGTGGAGA TTACTACCTT
    TCTGCAGAAG TCTTATCAAA ATTTAAACTA CTAGATGGAT CTCAGTGGAA TGAAGAACAC
    TTAAAAAAAA TTCAGACTGA GGTTTTAAAG CCCTTACTTC TTTATTGGGC ACCAAGATTC
    TGTGTTACTC ACTCATCCTC AACAAAACAT GCTAGTGCCG AGCTGAAATT CTGGCACCTT
    CGTCAGGAGC AGCCGAGGAA GGATGTTGAC CCTTTCCCAC AAATGGCAAC CCTCTTGCCG
    TTAAGACCCA AATCTTGCAT TCCACAGCTA CCTGAGATGC AGAAAGAAGA ATCCAGTTTA
    CTACAACTTC CTAAATCTCC CAAGAAATCA CCTAGAGTAA AGACAGCAGT TTATAAACCT
    TGGAAGAGTG AGTCTTTGAA TCCAGTTTCT TCTAAGGATG ATGTGATTGA GAAAGGGTCA
    AGGCACATGT CAGAAAGCAG CAAAGTTATT CGTTTAACAT CTTTTACTGA CATTTCTGAA
    TGTCTGAAGC CCCAGCTGGA TAGAAGATAT ACCTATACAG AAGAACCTTT GATTAAAACC
    ATGGGTGATG GTACCTTGGG AGGATTTGAC ATGGAGAATT TGTTGCAATC TTTGTATGTA
    GAAAACAGAG CTGGATTCTT CTTTACTAAA TTCTGCGAGA ACTCAGGGAA CAAGTTGTGG
    AAGAACTGTG TGTACTTTTG GTTTGACCTA CAAGCTTATC ATCAGCTTTT CTACCAAGAA
    ACCCTTCACC CTTTTAAAGT ATGCAAGCAA GCTCAATATC TTTTTGCTAC ATACATTGCT
    CCTTCAGCCA CTCTTGACAT TGGACTTCAA CAGGAAAAGA AAAGAGAAAT TTATATGAAA
    ATACAACCAC CATTTGAAGA CCTTTTTGAT GCAGCAGAAG AATATATCCT CCTCCTCCTT
    CTTGAGCCTT GGACAAAGAT GATAAAATCA GACCAAGTTG CTTACAGAAA GGTGGAGCTG
    GTGGAAGAAA CTCGACAGCT GGATTCCACA TACTTGAGAA AGCTACAGGC TTTGCATAAA
    GAGACAGTTT TCAAGAAAGC TGAGGACACT ACTTCTGAAA TTGGAACTGG TATTTCACCA
    CTTCCTCATG TCTCTAAAAA CACAGAATAC TGGAATATGG TTCCTGAAGA ATATAGGCAT
    TTTAATTTTA ATGAACTGCT TAACAACAAA CTGGAGTTTG AACATTTCCG TCAGTTTCTT
    GAGGCTCATT CTTCAAGCAT GGACCTTATG TGCTGGACAG ACATTGAACA GTTCCGAAGA
    ATAACTTACA GAGATCGAAA GCAAAGGGAG GCAAAATCTA TATATATAAA AAACAAATAC
    CTCAATAAAA AGTATTTCTT TGGGCCCAAC AGTCCAGCTT CTGTGTATCA ACAGGACCAG
    ATAATGAATT TAAGTGGTGG CTGGGGAAAG ATTCTCCATG AGCAACTTGA TGCAGCTGTT
    CTAATCGAAA TTCAGAAGCA TGTCCTAAAT AGACTAGAAA ATGTTTGGTT GCCGTTGTTT
    CTTGCAAGTG AACAGTTTGC GGCACGTCAA AAGATAAAGG TACAGATAAA AGACATAGCT
    GAAGAGCTTT TACTACAAAA GCGTGAAAGG AAAATTGGAG TCTGGAAGCC TGTGGAGAGT
    AAGTGGATAT CTTCGTCTTG TGAAATCATT GCTTTCCGTA AAGCTTTATT GAATCCAGTT
    ACTGCAAGAC AATTTCAACG CTTTGTGGCT CTAAAAGGAG ATTTATTGGA AAATGGGGTG
    CTCTTTTGGC AAGAAGTACA AAAATATAAG GACTTGTGCC ATTCTCATTG TGATGAGTCT
    ATCATACACA AGAAGATTAT GACTATTATC AACTGCTTTA TTAATTCCAG CATCCCCCCA
    GCTTTACAAA TTGACATTCC AGTAGAGCAA GCCCAGAAGA TTATTGAACA CAGGAAGGAG
    TTGGGACCAT ATGTATTCAG AGAGGCACAG ATGACAATTT TTGGAGTTCT GTTTAAATTT
    TGGCCTCAGT TCTGTGAGTT TAGGAAGAAC TTAACTGATG AAAAAATTAT GAGTGTTTTG
    GAGAGAAGAC AAGAATATAG TAAGCAGAAA AAGAAAATGG CAGTCATAGA AGATGAAAAA
    TTTGTAAAGG ATGGAATCAA ACAATATTCG AGTACTTCAG GATCTGTTAT CAAAGCAGCT
    GCTTTAACTA GTGACCCAGG TTTAGGCATG CAAGCGTACG GCCGACAGCC AACCTGGTGC
    TACTCAAAGT ACATAGAAGC CTTAGAACAG GAAAGGATTC TTCTTAAAAT TCAAGAGGAA
    CTAGAAAGAA AGTTGTTTGC AGGAATTCTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215690.1
    CDS47..3739
    Translation

    Target ORF information:

    RefSeq Version XM_005215633.3
    Organism Bos taurus(cattle)
    Definition Bos taurus regulator of G-protein signaling 22 (RGS22), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215633.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGTC TATGGAGTTT TTGAAGTAGT TAACAATGCT
    CCACAACTTC TGGAAAAGCA GTTGAAAAAA ATTGTACTAA ACCAGCGACC TCGAAATCCT
    ATCTATGATG TTGTAAGGAA AGGAAAGAGT GATGTTAAAC CTGTTCAAGT GAACGCCCCT
    CACGAAGATG AGACCATTAA TGTCAACTAC AATATCATGT GTCTCAATCG TGAACAAGGT
    ATTCACTGGG TCAAAAAAGA AAGACTTCCA ACTTTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGGTTAG CCAAATTGAT TTCACAAGTA AGATGGAGTG AATCAGGCAT GAATATCATA
    TTAGGTACAG AGTTTTCACC CTGGGTCCTG AAAAAACCAC CCAGTCCACC ACCTCCTCCT
    GTTGAAGAAG ATGATGTTAT AATTATGAAG AAGTTCTACA TTTCTCTGGG CGAGGCCTCC
    TATACTCAAA CAAAAGATTG GTTTGCCTTA GCAAAACAAT GTCAGCAAAC AGTACCAACC
    TTTTCACTAC CATCTTGTGT ATTCCACAAC AAAATTGAAT CACCAGTTGT TTCATCTGCT
    TCTGAGAGTT TTATCTTCGA TGATGGAGTT CACCCCAGGA CGAAAAAGGG CCTATTAAAA
    ACCACTAGAT TAACCTCCGA ATTTGAAGAA GATGGGTCTG TATCTCTACA GGACACTCCT
    TCTCAAGCTC TTCTGAGGAT ATACCTTGAA AAGCAACAGA ATGTTGATGG AAGTCTGACA
    CTGCACTTCT CAACATGTGA CGAGTTTTTA AGATCTTATG TCTCCTTTAT CTTGAGAGTG
    GCGATTCATC AAATTCTTGG AGCAACACTT AGAGAAAGCC CAGATTACGT AAATTTCAGA
    AACGTAACAG AAGTGGTATT TGATGACATT GGGGGGCCTA CCTCTGGCAA GAATGTTTTG
    TTCAATGAAA TGCTAAGGGA AGAGTCGGGA GACATGTTAG AACCAGCAGT TTCAAAGCCT
    GACAGCATTG GACTCGACAG CAGGGCTGAT TGGTGTATTT CTCACAAAAC TTATGACATT
    GGTAATAGAA AGGAGTTTGA AAGATTTAAG AAATTTTTAA AAGGTACCTT AGGGGAGAAA
    TATTGGTGGC TATGGATGGA TATTGAGAGG TTAAAAGTTC TTAAGAATCC TGGAAGACAT
    CAAAGACATA TTGAGAAGAT GAAAAAATGC TTTCTAGTGA GCAGTGGAGA TTACTACCTT
    TCTGCAGAAG TCTTATCAAA ATTTAAACTA CTAGATGGAT CTCAGTGGAA TGAAGAACAC
    TTAAAAAAAA TTCAGACTGA GGTTTTAAAG CCCTTACTTC TTTATTGGGC ACCAAGATTC
    TGTGTTACTC ACTCATCCTC AACAAAACAT GCTAGTGCCG AGCTGAAATT CTGGCACCTT
    CGTCAGGAGC AGCCGAGGAA GGATGTTGAC CCTTTCCCAC AAATGGCAAC CCTCTTGCCG
    TTAAGACCCA AATCTTGCAT TCCACAGCTA CCTGAGATGC AGAAAGAAGA ATCCAGTTTA
    CTACAACTTC CTAAATCTCC CAAGAAATCA CCTAGAGTAA AGACAGCAGT TTATAAACCT
    TGGAAGAGTG AGTCTTTGAA TCCAGTTTCT TCTAAGGATG ATGTGATTGA GAAAGGGTCA
    AGGCACATGT CAGAAAGCAG CAAAGTTATT CGTTTAACAT CTTTTACTGA CATTTCTGAA
    TGTCTGAAGC CCCAGCTGGA TAGAAGATAT ACCTATACAG AAGAACCTTT GATTAAAACC
    ATGGGTGATG GTACCTTGGG AGGATTTGAC ATGGAGAATT TGTTGCAATC TTTGTATGTA
    GAAAACAGAG CTGGATTCTT CTTTACTAAA TTCTGCGAGA ACTCAGGGAA CAAGTTGTGG
    AAGAACTGTG TGTACTTTTG GTTTGACCTA CAAGCTTATC ATCAGCTTTT CTACCAAGAA
    ACCCTTCACC CTTTTAAAGT ATGCAAGCAA GCTCAATATC TTTTTGCTAC ATACATTGCT
    CCTTCAGCCA CTCTTGACAT TGGACTTCAA CAGGAAAAGA AAAGAGAAAT TTATATGAAA
    ATACAACCAC CATTTGAAGA CCTTTTTGAT GCAGCAGAAG AATATATCCT CCTCCTCCTT
    CTTGAGCCTT GGACAAAGAT GATAAAATCA GACCAAGTTG CTTACAGAAA GGTGGAGCTG
    GTGGAAGAAA CTCGACAGCT GGATTCCACA TACTTGAGAA AGCTACAGGC TTTGCATAAA
    GAGACAGTTT TCAAGAAAGC TGAGGACACT ACTTCTGAAA TTGGAACTGG TATTTCACCA
    CTTCCTCATG TCTCTAAAAA CACAGAATAC TGGAATATGG TTCCTGAAGA ATATAGGCAT
    TTTAATTTTA ATGAACTGCT TAACAACAAA CTGGAGTTTG AACATTTCCG TCAGTTTCTT
    GAGGCTCATT CTTCAAGCAT GGACCTTATG TGCTGGACAG ACATTGAACA GTTCCGAAGA
    ATAACTTACA GAGATCGAAA GCAAAGGGAG GCAAAATCTA TATATATAAA AAACAAATAC
    CTCAATAAAA AGTATTTCTT TGGGCCCAAC AGTCCAGCTT CTGTGTATCA ACAGGACCAG
    ATAATGAATT TAAGTGGTGG CTGGGGAAAG ATTCTCCATG AGCAACTTGA TGCAGCTGTT
    CTAATCGAAA TTCAGAAGCA TGTCCTAAAT AGACTAGAAA ATGTTTGGTT GCCGTTGTTT
    CTTGCAAGTG AACAGTTTGC GGCACGTCAA AAGATAAAGG TACAGATAAA AGACATAGCT
    GAAGAGCTTT TACTACAAAA GCGTGAAAGG AAAATTGGAG TCTGGAAGCC TGTGGAGAGT
    AAGTGGATAT CTTCGTCTTG TGAAATCATT GCTTTCCGTA AAGCTTTATT GAATCCAGTT
    ACTGCAAGAC AATTTCAACG CTTTGTGGCT CTAAAAGGAG ATTTATTGGA AAATGGGGTG
    CTCTTTTGGC AAGAAGTACA AAAATATAAG GACTTGTGCC ATTCTCATTG TGATGAGTCT
    ATCATACACA AGAAGATTAT GACTATTATC AACTGCTTTA TTAATTCCAG CATCCCCCCA
    GCTTTACAAA TTGACATTCC AGTAGAGCAA GCCCAGAAGA TTATTGAACA CAGGAAGGAG
    TTGGGACCAT ATGTATTCAG AGAGGCACAG ATGACAATTT TTGGAGTTCT GTTTAAATTT
    TGGCCTCAGT TCTGTGAGTT TAGGAAGAAC TTAACTGATG AAAAAATTAT GAGTGTTTTG
    GAGAGAAGAC AAGAATATAG TAAGCAGAAA AAGAAAATGG CAGTCATAGA AGATGAAAAA
    TTTGTAAAGG ATGGAATCAA ACAATATTCG AGTACTTCAG GATCTGTTAT CAAAGCAGCT
    GCTTTAACTA GTGACCCAGG TTTAGGCATG CAAGCGTACG GCCGACAGCC AACCTGGTGC
    TACTCAAAGT ACATAGAAGC CTTAGAACAG GAAAGGATTC TTCTTAAAAT TCAAGAGGAA
    CTAGAAAGAA AGTTGTTTGC AGGAATTCTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa07980
    Clone ID Related Accession (Same CDS sequence) XM_015466375.1 , XM_005215633.3 , XM_005215633.4
    Accession Version XM_005215633.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulator of G-protein signaling 22 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215633.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGTC TATGGAGTTT TTGAAGTAGT TAACAATGCT
    CCACAACTTC TGGAAAAGCA GTTGAAAAAA ATTGTACTAA ACCAGCGACC TCGAAATCCT
    ATCTATGATG TTGTAAGGAA AGGAAAGAGT GATGTTAAAC CTGTTCAAGT GAACGCCCCT
    CACGAAGATG AGACCATTAA TGTCAACTAC AATATCATGT GTCTCAATCG TGAACAAGGT
    ATTCACTGGG TCAAAAAAGA AAGACTTCCA ACTTTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGGTTAG CCAAATTGAT TTCACAAGTA AGATGGAGTG AATCAGGCAT GAATATCATA
    TTAGGTACAG AGTTTTCACC CTGGGTCCTG AAAAAACCAC CCAGTCCACC ACCTCCTCCT
    GTTGAAGAAG ATGATGTTAT AATTATGAAG AAGTTCTACA TTTCTCTGGG CGAGGCCTCC
    TATACTCAAA CAAAAGATTG GTTTGCCTTA GCAAAACAAT GTCAGCAAAC AGTACCAACC
    TTTTCACTAC CATCTTGTGT ATTCCACAAC AAAATTGAAT CACCAGTTGT TTCATCTGCT
    TCTGAGAGTT TTATCTTCGA TGATGGAGTT CACCCCAGGA CGAAAAAGGG CCTATTAAAA
    ACCACTAGAT TAACCTCCGA ATTTGAAGAA GATGGGTCTG TATCTCTACA GGACACTCCT
    TCTCAAGCTC TTCTGAGGAT ATACCTTGAA AAGCAACAGA ATGTTGATGG AAGTCTGACA
    CTGCACTTCT CAACATGTGA CGAGTTTTTA AGATCTTATG TCTCCTTTAT CTTGAGAGTG
    GCGATTCATC AAATTCTTGG AGCAACACTT AGAGAAAGCC CAGATTACGT AAATTTCAGA
    AACGTAACAG AAGTGGTATT TGATGACATT GGGGGGCCTA CCTCTGGCAA GAATGTTTTG
    TTCAATGAAA TGCTAAGGGA AGAGTCGGGA GACATGTTAG AACCAGCAGT TTCAAAGCCT
    GACAGCATTG GACTCGACAG CAGGGCTGAT TGGTGTATTT CTCACAAAAC TTATGACATT
    GGTAATAGAA AGGAGTTTGA AAGATTTAAG AAATTTTTAA AAGGTACCTT AGGGGAGAAA
    TATTGGTGGC TATGGATGGA TATTGAGAGG TTAAAAGTTC TTAAGAATCC TGGAAGACAT
    CAAAGACATA TTGAGAAGAT GAAAAAATGC TTTCTAGTGA GCAGTGGAGA TTACTACCTT
    TCTGCAGAAG TCTTATCAAA ATTTAAACTA CTAGATGGAT CTCAGTGGAA TGAAGAACAC
    TTAAAAAAAA TTCAGACTGA GGTTTTAAAG CCCTTACTTC TTTATTGGGC ACCAAGATTC
    TGTGTTACTC ACTCATCCTC AACAAAACAT GCTAGTGCCG AGCTGAAATT CTGGCACCTT
    CGTCAGGAGC AGCCGAGGAA GGATGTTGAC CCTTTCCCAC AAATGGCAAC CCTCTTGCCG
    TTAAGACCCA AATCTTGCAT TCCACAGCTA CCTGAGATGC AGAAAGAAGA ATCCAGTTTA
    CTACAACTTC CTAAATCTCC CAAGAAATCA CCTAGAGTAA AGACAGCAGT TTATAAACCT
    TGGAAGAGTG AGTCTTTGAA TCCAGTTTCT TCTAAGGATG ATGTGATTGA GAAAGGGTCA
    AGGCACATGT CAGAAAGCAG CAAAGTTATT CGTTTAACAT CTTTTACTGA CATTTCTGAA
    TGTCTGAAGC CCCAGCTGGA TAGAAGATAT ACCTATACAG AAGAACCTTT GATTAAAACC
    ATGGGTGATG GTACCTTGGG AGGATTTGAC ATGGAGAATT TGTTGCAATC TTTGTATGTA
    GAAAACAGAG CTGGATTCTT CTTTACTAAA TTCTGCGAGA ACTCAGGGAA CAAGTTGTGG
    AAGAACTGTG TGTACTTTTG GTTTGACCTA CAAGCTTATC ATCAGCTTTT CTACCAAGAA
    ACCCTTCACC CTTTTAAAGT ATGCAAGCAA GCTCAATATC TTTTTGCTAC ATACATTGCT
    CCTTCAGCCA CTCTTGACAT TGGACTTCAA CAGGAAAAGA AAAGAGAAAT TTATATGAAA
    ATACAACCAC CATTTGAAGA CCTTTTTGAT GCAGCAGAAG AATATATCCT CCTCCTCCTT
    CTTGAGCCTT GGACAAAGAT GATAAAATCA GACCAAGTTG CTTACAGAAA GGTGGAGCTG
    GTGGAAGAAA CTCGACAGCT GGATTCCACA TACTTGAGAA AGCTACAGGC TTTGCATAAA
    GAGACAGTTT TCAAGAAAGC TGAGGACACT ACTTCTGAAA TTGGAACTGG TATTTCACCA
    CTTCCTCATG TCTCTAAAAA CACAGAATAC TGGAATATGG TTCCTGAAGA ATATAGGCAT
    TTTAATTTTA ATGAACTGCT TAACAACAAA CTGGAGTTTG AACATTTCCG TCAGTTTCTT
    GAGGCTCATT CTTCAAGCAT GGACCTTATG TGCTGGACAG ACATTGAACA GTTCCGAAGA
    ATAACTTACA GAGATCGAAA GCAAAGGGAG GCAAAATCTA TATATATAAA AAACAAATAC
    CTCAATAAAA AGTATTTCTT TGGGCCCAAC AGTCCAGCTT CTGTGTATCA ACAGGACCAG
    ATAATGAATT TAAGTGGTGG CTGGGGAAAG ATTCTCCATG AGCAACTTGA TGCAGCTGTT
    CTAATCGAAA TTCAGAAGCA TGTCCTAAAT AGACTAGAAA ATGTTTGGTT GCCGTTGTTT
    CTTGCAAGTG AACAGTTTGC GGCACGTCAA AAGATAAAGG TACAGATAAA AGACATAGCT
    GAAGAGCTTT TACTACAAAA GCGTGAAAGG AAAATTGGAG TCTGGAAGCC TGTGGAGAGT
    AAGTGGATAT CTTCGTCTTG TGAAATCATT GCTTTCCGTA AAGCTTTATT GAATCCAGTT
    ACTGCAAGAC AATTTCAACG CTTTGTGGCT CTAAAAGGAG ATTTATTGGA AAATGGGGTG
    CTCTTTTGGC AAGAAGTACA AAAATATAAG GACTTGTGCC ATTCTCATTG TGATGAGTCT
    ATCATACACA AGAAGATTAT GACTATTATC AACTGCTTTA TTAATTCCAG CATCCCCCCA
    GCTTTACAAA TTGACATTCC AGTAGAGCAA GCCCAGAAGA TTATTGAACA CAGGAAGGAG
    TTGGGACCAT ATGTATTCAG AGAGGCACAG ATGACAATTT TTGGAGTTCT GTTTAAATTT
    TGGCCTCAGT TCTGTGAGTT TAGGAAGAAC TTAACTGATG AAAAAATTAT GAGTGTTTTG
    GAGAGAAGAC AAGAATATAG TAAGCAGAAA AAGAAAATGG CAGTCATAGA AGATGAAAAA
    TTTGTAAAGG ATGGAATCAA ACAATATTCG AGTACTTCAG GATCTGTTAT CAAAGCAGCT
    GCTTTAACTA GTGACCCAGG TTTAGGCATG CAAGCGTACG GCCGACAGCC AACCTGGTGC
    TACTCAAAGT ACATAGAAGC CTTAGAACAG GAAAGGATTC TTCTTAAAAT TCAAGAGGAA
    CTAGAAAGAA AGTTGTTTGC AGGAATTCTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215690.1
    CDS77..3769
    Translation

    Target ORF information:

    RefSeq Version XM_005215633.4
    Organism Bos taurus(cattle)
    Definition Bos taurus regulator of G protein signaling 22 (RGS22), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215633.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGTC TATGGAGTTT TTGAAGTAGT TAACAATGCT
    CCACAACTTC TGGAAAAGCA GTTGAAAAAA ATTGTACTAA ACCAGCGACC TCGAAATCCT
    ATCTATGATG TTGTAAGGAA AGGAAAGAGT GATGTTAAAC CTGTTCAAGT GAACGCCCCT
    CACGAAGATG AGACCATTAA TGTCAACTAC AATATCATGT GTCTCAATCG TGAACAAGGT
    ATTCACTGGG TCAAAAAAGA AAGACTTCCA ACTTTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGGTTAG CCAAATTGAT TTCACAAGTA AGATGGAGTG AATCAGGCAT GAATATCATA
    TTAGGTACAG AGTTTTCACC CTGGGTCCTG AAAAAACCAC CCAGTCCACC ACCTCCTCCT
    GTTGAAGAAG ATGATGTTAT AATTATGAAG AAGTTCTACA TTTCTCTGGG CGAGGCCTCC
    TATACTCAAA CAAAAGATTG GTTTGCCTTA GCAAAACAAT GTCAGCAAAC AGTACCAACC
    TTTTCACTAC CATCTTGTGT ATTCCACAAC AAAATTGAAT CACCAGTTGT TTCATCTGCT
    TCTGAGAGTT TTATCTTCGA TGATGGAGTT CACCCCAGGA CGAAAAAGGG CCTATTAAAA
    ACCACTAGAT TAACCTCCGA ATTTGAAGAA GATGGGTCTG TATCTCTACA GGACACTCCT
    TCTCAAGCTC TTCTGAGGAT ATACCTTGAA AAGCAACAGA ATGTTGATGG AAGTCTGACA
    CTGCACTTCT CAACATGTGA CGAGTTTTTA AGATCTTATG TCTCCTTTAT CTTGAGAGTG
    GCGATTCATC AAATTCTTGG AGCAACACTT AGAGAAAGCC CAGATTACGT AAATTTCAGA
    AACGTAACAG AAGTGGTATT TGATGACATT GGGGGGCCTA CCTCTGGCAA GAATGTTTTG
    TTCAATGAAA TGCTAAGGGA AGAGTCGGGA GACATGTTAG AACCAGCAGT TTCAAAGCCT
    GACAGCATTG GACTCGACAG CAGGGCTGAT TGGTGTATTT CTCACAAAAC TTATGACATT
    GGTAATAGAA AGGAGTTTGA AAGATTTAAG AAATTTTTAA AAGGTACCTT AGGGGAGAAA
    TATTGGTGGC TATGGATGGA TATTGAGAGG TTAAAAGTTC TTAAGAATCC TGGAAGACAT
    CAAAGACATA TTGAGAAGAT GAAAAAATGC TTTCTAGTGA GCAGTGGAGA TTACTACCTT
    TCTGCAGAAG TCTTATCAAA ATTTAAACTA CTAGATGGAT CTCAGTGGAA TGAAGAACAC
    TTAAAAAAAA TTCAGACTGA GGTTTTAAAG CCCTTACTTC TTTATTGGGC ACCAAGATTC
    TGTGTTACTC ACTCATCCTC AACAAAACAT GCTAGTGCCG AGCTGAAATT CTGGCACCTT
    CGTCAGGAGC AGCCGAGGAA GGATGTTGAC CCTTTCCCAC AAATGGCAAC CCTCTTGCCG
    TTAAGACCCA AATCTTGCAT TCCACAGCTA CCTGAGATGC AGAAAGAAGA ATCCAGTTTA
    CTACAACTTC CTAAATCTCC CAAGAAATCA CCTAGAGTAA AGACAGCAGT TTATAAACCT
    TGGAAGAGTG AGTCTTTGAA TCCAGTTTCT TCTAAGGATG ATGTGATTGA GAAAGGGTCA
    AGGCACATGT CAGAAAGCAG CAAAGTTATT CGTTTAACAT CTTTTACTGA CATTTCTGAA
    TGTCTGAAGC CCCAGCTGGA TAGAAGATAT ACCTATACAG AAGAACCTTT GATTAAAACC
    ATGGGTGATG GTACCTTGGG AGGATTTGAC ATGGAGAATT TGTTGCAATC TTTGTATGTA
    GAAAACAGAG CTGGATTCTT CTTTACTAAA TTCTGCGAGA ACTCAGGGAA CAAGTTGTGG
    AAGAACTGTG TGTACTTTTG GTTTGACCTA CAAGCTTATC ATCAGCTTTT CTACCAAGAA
    ACCCTTCACC CTTTTAAAGT ATGCAAGCAA GCTCAATATC TTTTTGCTAC ATACATTGCT
    CCTTCAGCCA CTCTTGACAT TGGACTTCAA CAGGAAAAGA AAAGAGAAAT TTATATGAAA
    ATACAACCAC CATTTGAAGA CCTTTTTGAT GCAGCAGAAG AATATATCCT CCTCCTCCTT
    CTTGAGCCTT GGACAAAGAT GATAAAATCA GACCAAGTTG CTTACAGAAA GGTGGAGCTG
    GTGGAAGAAA CTCGACAGCT GGATTCCACA TACTTGAGAA AGCTACAGGC TTTGCATAAA
    GAGACAGTTT TCAAGAAAGC TGAGGACACT ACTTCTGAAA TTGGAACTGG TATTTCACCA
    CTTCCTCATG TCTCTAAAAA CACAGAATAC TGGAATATGG TTCCTGAAGA ATATAGGCAT
    TTTAATTTTA ATGAACTGCT TAACAACAAA CTGGAGTTTG AACATTTCCG TCAGTTTCTT
    GAGGCTCATT CTTCAAGCAT GGACCTTATG TGCTGGACAG ACATTGAACA GTTCCGAAGA
    ATAACTTACA GAGATCGAAA GCAAAGGGAG GCAAAATCTA TATATATAAA AAACAAATAC
    CTCAATAAAA AGTATTTCTT TGGGCCCAAC AGTCCAGCTT CTGTGTATCA ACAGGACCAG
    ATAATGAATT TAAGTGGTGG CTGGGGAAAG ATTCTCCATG AGCAACTTGA TGCAGCTGTT
    CTAATCGAAA TTCAGAAGCA TGTCCTAAAT AGACTAGAAA ATGTTTGGTT GCCGTTGTTT
    CTTGCAAGTG AACAGTTTGC GGCACGTCAA AAGATAAAGG TACAGATAAA AGACATAGCT
    GAAGAGCTTT TACTACAAAA GCGTGAAAGG AAAATTGGAG TCTGGAAGCC TGTGGAGAGT
    AAGTGGATAT CTTCGTCTTG TGAAATCATT GCTTTCCGTA AAGCTTTATT GAATCCAGTT
    ACTGCAAGAC AATTTCAACG CTTTGTGGCT CTAAAAGGAG ATTTATTGGA AAATGGGGTG
    CTCTTTTGGC AAGAAGTACA AAAATATAAG GACTTGTGCC ATTCTCATTG TGATGAGTCT
    ATCATACACA AGAAGATTAT GACTATTATC AACTGCTTTA TTAATTCCAG CATCCCCCCA
    GCTTTACAAA TTGACATTCC AGTAGAGCAA GCCCAGAAGA TTATTGAACA CAGGAAGGAG
    TTGGGACCAT ATGTATTCAG AGAGGCACAG ATGACAATTT TTGGAGTTCT GTTTAAATTT
    TGGCCTCAGT TCTGTGAGTT TAGGAAGAAC TTAACTGATG AAAAAATTAT GAGTGTTTTG
    GAGAGAAGAC AAGAATATAG TAAGCAGAAA AAGAAAATGG CAGTCATAGA AGATGAAAAA
    TTTGTAAAGG ATGGAATCAA ACAATATTCG AGTACTTCAG GATCTGTTAT CAAAGCAGCT
    GCTTTAACTA GTGACCCAGG TTTAGGCATG CAAGCGTACG GCCGACAGCC AACCTGGTGC
    TACTCAAAGT ACATAGAAGC CTTAGAACAG GAAAGGATTC TTCTTAAAAT TCAAGAGGAA
    CTAGAAAGAA AGTTGTTTGC AGGAATTCTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26089
    Clone ID Related Accession (Same CDS sequence) XM_015466374.1 , XM_002692801.4 , XM_002692801.5
    Accession Version XM_015466374.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulator of G-protein signaling 22 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646230.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGTC TATGGAGTTT TTGAAGTAGT TAACAATGCT
    CCACAACTTC TGGAAAAGCA GTTGAAAAAA ATTGTACTAA ACCAGCGACC TCGAAATCCT
    ATCTATGATG TTGTAAGGAA AGGAAAGAGT GATGTTAAAC CTGTTCAAGT GAACGCCCCT
    CACGAAGATG AGACCATTAA TGTCAACTAC AATATCATGT GTCTCAATCG TGAACAAGGT
    ATTCACTGGG TCAAAAAAGA AAGACTTCCA ACTTTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGGTTAG CCAAATTGAT TTCACAAGTA AGATGGAGTG AATCAGGCAT GAATATCATA
    TTAGGTACAG AGTTTTCACC CTGGGTCCTG AAAAAACCAC CCAGTCCACC ACCTCCTCCT
    GTTGAAGAAG ATGATGTTAT AATTATGAAG AAGTTCTACA TTTCTCTGGG CGAGGCCTCC
    TATACTCAAA CAAAAGATTG GTTTGCCTTA GCAAAACAAT GTCAGCAAAC AGTACCAACC
    TTTTCACTAC CATCTTGTGT ATTCCACAAC AAAATTGAAT CACCAGTTGT TTCATCTGCT
    TCTGAGAGTT TTATCTTCGA TGATGGAGTT CACCCCAGGA CGAAAAAGGG CCTATTAAAA
    ACCACTAGAT TAACCTCCGA ATTTGAAGAA GATGGGTCTG TATCTCTACA GGACACTCCT
    TCTCAAGCTC TTCTGAGGAT ATACCTTGAA AAGCAACAGA ATGTTGATGG AAGTCTGACA
    CTGCACTTCT CAACATGTGA CGAGTTTTTA AGATCTTATG TCTCCTTTAT CTTGAGAGTG
    GCGATTCATC AAATTCTTGG AGCAACACTT AGAGAAAGCC CAGATTACGT AAATTTCAGA
    AACGTAACAG AAGTGGTATT TGATGACATT GGGGGGCCTA CCTCTGGCAA GAATGTTTTG
    TTCAATGAAA TGCTAAGGGA AGAGTCGGGA GACATGTTAG AACCAGCAGT TTCAAAGCCT
    GACAGCATTG GACTCGACAG CAGGGCTGAT TGGTGTATTT CTCACAAAAC TTATGACATT
    GGTAATAGAA AGGAGTTTGA AAGATTTAAG AAATTTTTAA AAGGTACCTT AGGGGAGAAA
    TATTGGTGGC TATGGATGGA TATTGAGAGG TTAAAAGTTC TTAAGAATCC TGGAAGACAT
    CAAAGACATA TTGAGAAGAT GAAAAAATGC TTTCTAGTGA GCAGTGGAGA TTACTACCTT
    TCTGCAGAAG TCTTATCAAA ATTTAAACTA CTAGATGGAT CTCAGTGGAA TGAAGAACAC
    TTAAAAAAAA TTCAGACTGA GGTTTTAAAG CCCTTACTTC TTTATTGGGC ACCAAGATTC
    TGTGTTACTC ACTCATCCTC AACAAAACAT GCTAGTGCCG AGCTGAAATT CTGGCACCTT
    CGTCAGGAGC AGCCGAGGAA GGATGTTGAC CCTTTCCCAC AAATGGCAAC CCTCTTGCCG
    TTAAGACCCA AATCTTGCAT TCCACAGCTA CCTGAGATGC AGAAAGAAGA ATCCAGTTTA
    CTACAACTTC CTAAATCTCC CAAGAAATCA CCTAGAGTAA AGACAGCAGT TTATAAACCT
    TGGAAGAGTG AGTCTTTGAA TCCAGTTTCT TCTAAGGATG ATGTGATTGA GAAAGGGTCA
    AGGCACATGT CAGAAAGCAG CAAAGTTATT CGTTTAACAT CTTTTACTGA CATTTCTGAA
    TGTCTGAAGC CCCAGCTGGA TAGAAGATAT ACCTATACAG AAGAACCTTT GATTAAAACC
    ATGGGTGATG GTACCTTGGG AGGATTTGAC ATGGAGAATT TGTTGCAATC TTTGTATGTA
    GAAAACAGAG CTGGATTCTT CTTTACTAAA TTCTGCGAGA ACTCAGGGAA CAAGTTGTGG
    AAGAACTGTG TGTACTTTTG GTTTGACCTA CAAGCTTATC ATCAGCTTTT CTACCAAGAA
    ACCCTTCACC CTTTTAAAGT ATGCAAGCAA GCTCAATATC TTTTTGCTAC ATACATTGCT
    CCTTCAGCCA CTCTTGACAT TGGACTTCAA CAGGAAAAGA AAAGAGAAAT TTATATGAAA
    ATACAACCAC CATTTGAAGA CCTTTTTGAT GCAGCAGAAG AATATATCCT CCTCCTCCTT
    CTTGAGCCTT GGACAAAGAT GATAAAATCA GACCAAGTTG CTTACAGAAA GGTGGAGCTG
    GTGGAAGAAA CTCGACAGCT GGATTCCACA TACTTGAGAA AGCTACAGGC TTTGCATAAA
    GAGACAGTTT TCAAGAAAGC TGAGGACACT ACTTCTGAAA TTGGAACTGG TATTTCACCA
    CTTCCTCATG TCTCTAAAAA CACAGAATAC TGGAATATGG TTCCTGAAGA ATATAGGCAT
    TTTAATTTTA ATGAACTGCT TAACAACAAA CTGGAGTTTG AACATTTCCG TCAGTTTCTT
    GAGGCTCATT CTTCAAGCAT GGACCTTATG TGCTGGACAG ACATTGAACA GTTCCGAAGA
    ATAACTTACA GAGATCGAAA GCAAAGGGAG GCAAAATCTA TATATATAAA AAACAAATAC
    CTCAATAAAA AGTATTTCTT TGGGCCCAAC AGTCCAGCTT CTGTGTATCA ACAGGACCAG
    ATAATGAATT TAAGTGGTGG CTGGGGAAAG ATTCTCCATG AGCAACTTGA TGCAGCTGTT
    CTAATCGAAA TTCAGAAGCA TGTCCTAAAT AGACTAGAAA ATGTTTGGTT GCCGTTGTTT
    CTTGCAAGTG AACAGTTTGC GGCACGTCAA AAGATAAAGG TACAGATAAA AGACATAGCT
    GAAGAGCTTT TACTACAAAA GCGTGAAAGG AAAATTGGAG TCTGGAAGCC TGTGGAGAGT
    AAGTGGATAT CTTCGTCTTG TGAAATCATT GCTTTCCGTA AAGCTTTATT GAATCCAGTT
    ACTGCAAGAC AATTTCAACG CTTTGTGGCT CTAAAAGGAG ATTTATTGGA AAATGGGGTG
    CTCTTTTGGC AAGAAGTACA AAAATATAAG GACTTGTGCC ATTCTCATTG TGATGAGTCT
    ATCATACACA AGAAGATTAT GACTATTATC AACTGCTTTA TTAATTCCAG CATCCCCCCA
    GCTTTACAAA TTGACATTCC AGTAGAGCAA GCCCAGAAGA TTATTGAACA CAGGAAGGAG
    TTGGGACCAT ATGTATTCAG AGAGGCACAG ATGACAATTT TTGGAGTTCT GTTTAAATTT
    TGGCCTCAGT TCTGTGAGTT TAGGAAGAAC TTAACTGATG AAAAAATTAT GAGTGTTTTG
    GAGAGAAGAC AAGAATATAG TAAGCAGAAA AAGAAAATGG CAGTCATAGA AGATGAAAAA
    TTTGTAAAGG ATGGAATCAA ACAATATTCG AGTACTTCAG GATCTGTTAT CAAAGCAGCT
    GCTTTAACTA GTGACCCAGG TTTAGGCATG CAAGCGTACG GCCGACAGCC AACCTGGTGC
    TACTCAAAGT ACATAGAAGC CTTAGAACAG GAAAGGATTC TTCTTAAAAT TCAAGAGGAA
    CTAGAAAGAA AGTTGTTTGC AGCCACACCA TCTTTTACAA ATTTCAAGTC TACTGCTTCC
    ACTCTGTCTT TGAAAAAGAC TGTGTCCTCC CACAACATCC AGAGAATTCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321860.1
    CDS47..3820
    Translation

    Target ORF information:

    RefSeq Version XM_015466374.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulator of G-protein signaling 22 (RGS22), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466374.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGTC TATGGAGTTT TTGAAGTAGT TAACAATGCT
    CCACAACTTC TGGAAAAGCA GTTGAAAAAA ATTGTACTAA ACCAGCGACC TCGAAATCCT
    ATCTATGATG TTGTAAGGAA AGGAAAGAGT GATGTTAAAC CTGTTCAAGT GAACGCCCCT
    CACGAAGATG AGACCATTAA TGTCAACTAC AATATCATGT GTCTCAATCG TGAACAAGGT
    ATTCACTGGG TCAAAAAAGA AAGACTTCCA ACTTTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGGTTAG CCAAATTGAT TTCACAAGTA AGATGGAGTG AATCAGGCAT GAATATCATA
    TTAGGTACAG AGTTTTCACC CTGGGTCCTG AAAAAACCAC CCAGTCCACC ACCTCCTCCT
    GTTGAAGAAG ATGATGTTAT AATTATGAAG AAGTTCTACA TTTCTCTGGG CGAGGCCTCC
    TATACTCAAA CAAAAGATTG GTTTGCCTTA GCAAAACAAT GTCAGCAAAC AGTACCAACC
    TTTTCACTAC CATCTTGTGT ATTCCACAAC AAAATTGAAT CACCAGTTGT TTCATCTGCT
    TCTGAGAGTT TTATCTTCGA TGATGGAGTT CACCCCAGGA CGAAAAAGGG CCTATTAAAA
    ACCACTAGAT TAACCTCCGA ATTTGAAGAA GATGGGTCTG TATCTCTACA GGACACTCCT
    TCTCAAGCTC TTCTGAGGAT ATACCTTGAA AAGCAACAGA ATGTTGATGG AAGTCTGACA
    CTGCACTTCT CAACATGTGA CGAGTTTTTA AGATCTTATG TCTCCTTTAT CTTGAGAGTG
    GCGATTCATC AAATTCTTGG AGCAACACTT AGAGAAAGCC CAGATTACGT AAATTTCAGA
    AACGTAACAG AAGTGGTATT TGATGACATT GGGGGGCCTA CCTCTGGCAA GAATGTTTTG
    TTCAATGAAA TGCTAAGGGA AGAGTCGGGA GACATGTTAG AACCAGCAGT TTCAAAGCCT
    GACAGCATTG GACTCGACAG CAGGGCTGAT TGGTGTATTT CTCACAAAAC TTATGACATT
    GGTAATAGAA AGGAGTTTGA AAGATTTAAG AAATTTTTAA AAGGTACCTT AGGGGAGAAA
    TATTGGTGGC TATGGATGGA TATTGAGAGG TTAAAAGTTC TTAAGAATCC TGGAAGACAT
    CAAAGACATA TTGAGAAGAT GAAAAAATGC TTTCTAGTGA GCAGTGGAGA TTACTACCTT
    TCTGCAGAAG TCTTATCAAA ATTTAAACTA CTAGATGGAT CTCAGTGGAA TGAAGAACAC
    TTAAAAAAAA TTCAGACTGA GGTTTTAAAG CCCTTACTTC TTTATTGGGC ACCAAGATTC
    TGTGTTACTC ACTCATCCTC AACAAAACAT GCTAGTGCCG AGCTGAAATT CTGGCACCTT
    CGTCAGGAGC AGCCGAGGAA GGATGTTGAC CCTTTCCCAC AAATGGCAAC CCTCTTGCCG
    TTAAGACCCA AATCTTGCAT TCCACAGCTA CCTGAGATGC AGAAAGAAGA ATCCAGTTTA
    CTACAACTTC CTAAATCTCC CAAGAAATCA CCTAGAGTAA AGACAGCAGT TTATAAACCT
    TGGAAGAGTG AGTCTTTGAA TCCAGTTTCT TCTAAGGATG ATGTGATTGA GAAAGGGTCA
    AGGCACATGT CAGAAAGCAG CAAAGTTATT CGTTTAACAT CTTTTACTGA CATTTCTGAA
    TGTCTGAAGC CCCAGCTGGA TAGAAGATAT ACCTATACAG AAGAACCTTT GATTAAAACC
    ATGGGTGATG GTACCTTGGG AGGATTTGAC ATGGAGAATT TGTTGCAATC TTTGTATGTA
    GAAAACAGAG CTGGATTCTT CTTTACTAAA TTCTGCGAGA ACTCAGGGAA CAAGTTGTGG
    AAGAACTGTG TGTACTTTTG GTTTGACCTA CAAGCTTATC ATCAGCTTTT CTACCAAGAA
    ACCCTTCACC CTTTTAAAGT ATGCAAGCAA GCTCAATATC TTTTTGCTAC ATACATTGCT
    CCTTCAGCCA CTCTTGACAT TGGACTTCAA CAGGAAAAGA AAAGAGAAAT TTATATGAAA
    ATACAACCAC CATTTGAAGA CCTTTTTGAT GCAGCAGAAG AATATATCCT CCTCCTCCTT
    CTTGAGCCTT GGACAAAGAT GATAAAATCA GACCAAGTTG CTTACAGAAA GGTGGAGCTG
    GTGGAAGAAA CTCGACAGCT GGATTCCACA TACTTGAGAA AGCTACAGGC TTTGCATAAA
    GAGACAGTTT TCAAGAAAGC TGAGGACACT ACTTCTGAAA TTGGAACTGG TATTTCACCA
    CTTCCTCATG TCTCTAAAAA CACAGAATAC TGGAATATGG TTCCTGAAGA ATATAGGCAT
    TTTAATTTTA ATGAACTGCT TAACAACAAA CTGGAGTTTG AACATTTCCG TCAGTTTCTT
    GAGGCTCATT CTTCAAGCAT GGACCTTATG TGCTGGACAG ACATTGAACA GTTCCGAAGA
    ATAACTTACA GAGATCGAAA GCAAAGGGAG GCAAAATCTA TATATATAAA AAACAAATAC
    CTCAATAAAA AGTATTTCTT TGGGCCCAAC AGTCCAGCTT CTGTGTATCA ACAGGACCAG
    ATAATGAATT TAAGTGGTGG CTGGGGAAAG ATTCTCCATG AGCAACTTGA TGCAGCTGTT
    CTAATCGAAA TTCAGAAGCA TGTCCTAAAT AGACTAGAAA ATGTTTGGTT GCCGTTGTTT
    CTTGCAAGTG AACAGTTTGC GGCACGTCAA AAGATAAAGG TACAGATAAA AGACATAGCT
    GAAGAGCTTT TACTACAAAA GCGTGAAAGG AAAATTGGAG TCTGGAAGCC TGTGGAGAGT
    AAGTGGATAT CTTCGTCTTG TGAAATCATT GCTTTCCGTA AAGCTTTATT GAATCCAGTT
    ACTGCAAGAC AATTTCAACG CTTTGTGGCT CTAAAAGGAG ATTTATTGGA AAATGGGGTG
    CTCTTTTGGC AAGAAGTACA AAAATATAAG GACTTGTGCC ATTCTCATTG TGATGAGTCT
    ATCATACACA AGAAGATTAT GACTATTATC AACTGCTTTA TTAATTCCAG CATCCCCCCA
    GCTTTACAAA TTGACATTCC AGTAGAGCAA GCCCAGAAGA TTATTGAACA CAGGAAGGAG
    TTGGGACCAT ATGTATTCAG AGAGGCACAG ATGACAATTT TTGGAGTTCT GTTTAAATTT
    TGGCCTCAGT TCTGTGAGTT TAGGAAGAAC TTAACTGATG AAAAAATTAT GAGTGTTTTG
    GAGAGAAGAC AAGAATATAG TAAGCAGAAA AAGAAAATGG CAGTCATAGA AGATGAAAAA
    TTTGTAAAGG ATGGAATCAA ACAATATTCG AGTACTTCAG GATCTGTTAT CAAAGCAGCT
    GCTTTAACTA GTGACCCAGG TTTAGGCATG CAAGCGTACG GCCGACAGCC AACCTGGTGC
    TACTCAAAGT ACATAGAAGC CTTAGAACAG GAAAGGATTC TTCTTAAAAT TCAAGAGGAA
    CTAGAAAGAA AGTTGTTTGC AGCCACACCA TCTTTTACAA ATTTCAAGTC TACTGCTTCC
    ACTCTGTCTT TGAAAAAGAC TGTGTCCTCC CACAACATCC AGAGAATTCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26089
    Clone ID Related Accession (Same CDS sequence) XM_015466374.1 , XM_002692801.4 , XM_002692801.5
    Accession Version XM_002692801.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulator of G-protein signaling 22 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104399.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002692801.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGTC TATGGAGTTT TTGAAGTAGT TAACAATGCT
    CCACAACTTC TGGAAAAGCA GTTGAAAAAA ATTGTACTAA ACCAGCGACC TCGAAATCCT
    ATCTATGATG TTGTAAGGAA AGGAAAGAGT GATGTTAAAC CTGTTCAAGT GAACGCCCCT
    CACGAAGATG AGACCATTAA TGTCAACTAC AATATCATGT GTCTCAATCG TGAACAAGGT
    ATTCACTGGG TCAAAAAAGA AAGACTTCCA ACTTTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGGTTAG CCAAATTGAT TTCACAAGTA AGATGGAGTG AATCAGGCAT GAATATCATA
    TTAGGTACAG AGTTTTCACC CTGGGTCCTG AAAAAACCAC CCAGTCCACC ACCTCCTCCT
    GTTGAAGAAG ATGATGTTAT AATTATGAAG AAGTTCTACA TTTCTCTGGG CGAGGCCTCC
    TATACTCAAA CAAAAGATTG GTTTGCCTTA GCAAAACAAT GTCAGCAAAC AGTACCAACC
    TTTTCACTAC CATCTTGTGT ATTCCACAAC AAAATTGAAT CACCAGTTGT TTCATCTGCT
    TCTGAGAGTT TTATCTTCGA TGATGGAGTT CACCCCAGGA CGAAAAAGGG CCTATTAAAA
    ACCACTAGAT TAACCTCCGA ATTTGAAGAA GATGGGTCTG TATCTCTACA GGACACTCCT
    TCTCAAGCTC TTCTGAGGAT ATACCTTGAA AAGCAACAGA ATGTTGATGG AAGTCTGACA
    CTGCACTTCT CAACATGTGA CGAGTTTTTA AGATCTTATG TCTCCTTTAT CTTGAGAGTG
    GCGATTCATC AAATTCTTGG AGCAACACTT AGAGAAAGCC CAGATTACGT AAATTTCAGA
    AACGTAACAG AAGTGGTATT TGATGACATT GGGGGGCCTA CCTCTGGCAA GAATGTTTTG
    TTCAATGAAA TGCTAAGGGA AGAGTCGGGA GACATGTTAG AACCAGCAGT TTCAAAGCCT
    GACAGCATTG GACTCGACAG CAGGGCTGAT TGGTGTATTT CTCACAAAAC TTATGACATT
    GGTAATAGAA AGGAGTTTGA AAGATTTAAG AAATTTTTAA AAGGTACCTT AGGGGAGAAA
    TATTGGTGGC TATGGATGGA TATTGAGAGG TTAAAAGTTC TTAAGAATCC TGGAAGACAT
    CAAAGACATA TTGAGAAGAT GAAAAAATGC TTTCTAGTGA GCAGTGGAGA TTACTACCTT
    TCTGCAGAAG TCTTATCAAA ATTTAAACTA CTAGATGGAT CTCAGTGGAA TGAAGAACAC
    TTAAAAAAAA TTCAGACTGA GGTTTTAAAG CCCTTACTTC TTTATTGGGC ACCAAGATTC
    TGTGTTACTC ACTCATCCTC AACAAAACAT GCTAGTGCCG AGCTGAAATT CTGGCACCTT
    CGTCAGGAGC AGCCGAGGAA GGATGTTGAC CCTTTCCCAC AAATGGCAAC CCTCTTGCCG
    TTAAGACCCA AATCTTGCAT TCCACAGCTA CCTGAGATGC AGAAAGAAGA ATCCAGTTTA
    CTACAACTTC CTAAATCTCC CAAGAAATCA CCTAGAGTAA AGACAGCAGT TTATAAACCT
    TGGAAGAGTG AGTCTTTGAA TCCAGTTTCT TCTAAGGATG ATGTGATTGA GAAAGGGTCA
    AGGCACATGT CAGAAAGCAG CAAAGTTATT CGTTTAACAT CTTTTACTGA CATTTCTGAA
    TGTCTGAAGC CCCAGCTGGA TAGAAGATAT ACCTATACAG AAGAACCTTT GATTAAAACC
    ATGGGTGATG GTACCTTGGG AGGATTTGAC ATGGAGAATT TGTTGCAATC TTTGTATGTA
    GAAAACAGAG CTGGATTCTT CTTTACTAAA TTCTGCGAGA ACTCAGGGAA CAAGTTGTGG
    AAGAACTGTG TGTACTTTTG GTTTGACCTA CAAGCTTATC ATCAGCTTTT CTACCAAGAA
    ACCCTTCACC CTTTTAAAGT ATGCAAGCAA GCTCAATATC TTTTTGCTAC ATACATTGCT
    CCTTCAGCCA CTCTTGACAT TGGACTTCAA CAGGAAAAGA AAAGAGAAAT TTATATGAAA
    ATACAACCAC CATTTGAAGA CCTTTTTGAT GCAGCAGAAG AATATATCCT CCTCCTCCTT
    CTTGAGCCTT GGACAAAGAT GATAAAATCA GACCAAGTTG CTTACAGAAA GGTGGAGCTG
    GTGGAAGAAA CTCGACAGCT GGATTCCACA TACTTGAGAA AGCTACAGGC TTTGCATAAA
    GAGACAGTTT TCAAGAAAGC TGAGGACACT ACTTCTGAAA TTGGAACTGG TATTTCACCA
    CTTCCTCATG TCTCTAAAAA CACAGAATAC TGGAATATGG TTCCTGAAGA ATATAGGCAT
    TTTAATTTTA ATGAACTGCT TAACAACAAA CTGGAGTTTG AACATTTCCG TCAGTTTCTT
    GAGGCTCATT CTTCAAGCAT GGACCTTATG TGCTGGACAG ACATTGAACA GTTCCGAAGA
    ATAACTTACA GAGATCGAAA GCAAAGGGAG GCAAAATCTA TATATATAAA AAACAAATAC
    CTCAATAAAA AGTATTTCTT TGGGCCCAAC AGTCCAGCTT CTGTGTATCA ACAGGACCAG
    ATAATGAATT TAAGTGGTGG CTGGGGAAAG ATTCTCCATG AGCAACTTGA TGCAGCTGTT
    CTAATCGAAA TTCAGAAGCA TGTCCTAAAT AGACTAGAAA ATGTTTGGTT GCCGTTGTTT
    CTTGCAAGTG AACAGTTTGC GGCACGTCAA AAGATAAAGG TACAGATAAA AGACATAGCT
    GAAGAGCTTT TACTACAAAA GCGTGAAAGG AAAATTGGAG TCTGGAAGCC TGTGGAGAGT
    AAGTGGATAT CTTCGTCTTG TGAAATCATT GCTTTCCGTA AAGCTTTATT GAATCCAGTT
    ACTGCAAGAC AATTTCAACG CTTTGTGGCT CTAAAAGGAG ATTTATTGGA AAATGGGGTG
    CTCTTTTGGC AAGAAGTACA AAAATATAAG GACTTGTGCC ATTCTCATTG TGATGAGTCT
    ATCATACACA AGAAGATTAT GACTATTATC AACTGCTTTA TTAATTCCAG CATCCCCCCA
    GCTTTACAAA TTGACATTCC AGTAGAGCAA GCCCAGAAGA TTATTGAACA CAGGAAGGAG
    TTGGGACCAT ATGTATTCAG AGAGGCACAG ATGACAATTT TTGGAGTTCT GTTTAAATTT
    TGGCCTCAGT TCTGTGAGTT TAGGAAGAAC TTAACTGATG AAAAAATTAT GAGTGTTTTG
    GAGAGAAGAC AAGAATATAG TAAGCAGAAA AAGAAAATGG CAGTCATAGA AGATGAAAAA
    TTTGTAAAGG ATGGAATCAA ACAATATTCG AGTACTTCAG GATCTGTTAT CAAAGCAGCT
    GCTTTAACTA GTGACCCAGG TTTAGGCATG CAAGCGTACG GCCGACAGCC AACCTGGTGC
    TACTCAAAGT ACATAGAAGC CTTAGAACAG GAAAGGATTC TTCTTAAAAT TCAAGAGGAA
    CTAGAAAGAA AGTTGTTTGC AGCCACACCA TCTTTTACAA ATTTCAAGTC TACTGCTTCC
    ACTCTGTCTT TGAAAAAGAC TGTGTCCTCC CACAACATCC AGAGAATTCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002692847.2
    CDS47..3820
    Translation

    Target ORF information:

    RefSeq Version XM_002692801.4
    Organism Bos taurus(cattle)
    Definition Bos taurus regulator of G-protein signaling 22 (RGS22), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002692801.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGTC TATGGAGTTT TTGAAGTAGT TAACAATGCT
    CCACAACTTC TGGAAAAGCA GTTGAAAAAA ATTGTACTAA ACCAGCGACC TCGAAATCCT
    ATCTATGATG TTGTAAGGAA AGGAAAGAGT GATGTTAAAC CTGTTCAAGT GAACGCCCCT
    CACGAAGATG AGACCATTAA TGTCAACTAC AATATCATGT GTCTCAATCG TGAACAAGGT
    ATTCACTGGG TCAAAAAAGA AAGACTTCCA ACTTTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGGTTAG CCAAATTGAT TTCACAAGTA AGATGGAGTG AATCAGGCAT GAATATCATA
    TTAGGTACAG AGTTTTCACC CTGGGTCCTG AAAAAACCAC CCAGTCCACC ACCTCCTCCT
    GTTGAAGAAG ATGATGTTAT AATTATGAAG AAGTTCTACA TTTCTCTGGG CGAGGCCTCC
    TATACTCAAA CAAAAGATTG GTTTGCCTTA GCAAAACAAT GTCAGCAAAC AGTACCAACC
    TTTTCACTAC CATCTTGTGT ATTCCACAAC AAAATTGAAT CACCAGTTGT TTCATCTGCT
    TCTGAGAGTT TTATCTTCGA TGATGGAGTT CACCCCAGGA CGAAAAAGGG CCTATTAAAA
    ACCACTAGAT TAACCTCCGA ATTTGAAGAA GATGGGTCTG TATCTCTACA GGACACTCCT
    TCTCAAGCTC TTCTGAGGAT ATACCTTGAA AAGCAACAGA ATGTTGATGG AAGTCTGACA
    CTGCACTTCT CAACATGTGA CGAGTTTTTA AGATCTTATG TCTCCTTTAT CTTGAGAGTG
    GCGATTCATC AAATTCTTGG AGCAACACTT AGAGAAAGCC CAGATTACGT AAATTTCAGA
    AACGTAACAG AAGTGGTATT TGATGACATT GGGGGGCCTA CCTCTGGCAA GAATGTTTTG
    TTCAATGAAA TGCTAAGGGA AGAGTCGGGA GACATGTTAG AACCAGCAGT TTCAAAGCCT
    GACAGCATTG GACTCGACAG CAGGGCTGAT TGGTGTATTT CTCACAAAAC TTATGACATT
    GGTAATAGAA AGGAGTTTGA AAGATTTAAG AAATTTTTAA AAGGTACCTT AGGGGAGAAA
    TATTGGTGGC TATGGATGGA TATTGAGAGG TTAAAAGTTC TTAAGAATCC TGGAAGACAT
    CAAAGACATA TTGAGAAGAT GAAAAAATGC TTTCTAGTGA GCAGTGGAGA TTACTACCTT
    TCTGCAGAAG TCTTATCAAA ATTTAAACTA CTAGATGGAT CTCAGTGGAA TGAAGAACAC
    TTAAAAAAAA TTCAGACTGA GGTTTTAAAG CCCTTACTTC TTTATTGGGC ACCAAGATTC
    TGTGTTACTC ACTCATCCTC AACAAAACAT GCTAGTGCCG AGCTGAAATT CTGGCACCTT
    CGTCAGGAGC AGCCGAGGAA GGATGTTGAC CCTTTCCCAC AAATGGCAAC CCTCTTGCCG
    TTAAGACCCA AATCTTGCAT TCCACAGCTA CCTGAGATGC AGAAAGAAGA ATCCAGTTTA
    CTACAACTTC CTAAATCTCC CAAGAAATCA CCTAGAGTAA AGACAGCAGT TTATAAACCT
    TGGAAGAGTG AGTCTTTGAA TCCAGTTTCT TCTAAGGATG ATGTGATTGA GAAAGGGTCA
    AGGCACATGT CAGAAAGCAG CAAAGTTATT CGTTTAACAT CTTTTACTGA CATTTCTGAA
    TGTCTGAAGC CCCAGCTGGA TAGAAGATAT ACCTATACAG AAGAACCTTT GATTAAAACC
    ATGGGTGATG GTACCTTGGG AGGATTTGAC ATGGAGAATT TGTTGCAATC TTTGTATGTA
    GAAAACAGAG CTGGATTCTT CTTTACTAAA TTCTGCGAGA ACTCAGGGAA CAAGTTGTGG
    AAGAACTGTG TGTACTTTTG GTTTGACCTA CAAGCTTATC ATCAGCTTTT CTACCAAGAA
    ACCCTTCACC CTTTTAAAGT ATGCAAGCAA GCTCAATATC TTTTTGCTAC ATACATTGCT
    CCTTCAGCCA CTCTTGACAT TGGACTTCAA CAGGAAAAGA AAAGAGAAAT TTATATGAAA
    ATACAACCAC CATTTGAAGA CCTTTTTGAT GCAGCAGAAG AATATATCCT CCTCCTCCTT
    CTTGAGCCTT GGACAAAGAT GATAAAATCA GACCAAGTTG CTTACAGAAA GGTGGAGCTG
    GTGGAAGAAA CTCGACAGCT GGATTCCACA TACTTGAGAA AGCTACAGGC TTTGCATAAA
    GAGACAGTTT TCAAGAAAGC TGAGGACACT ACTTCTGAAA TTGGAACTGG TATTTCACCA
    CTTCCTCATG TCTCTAAAAA CACAGAATAC TGGAATATGG TTCCTGAAGA ATATAGGCAT
    TTTAATTTTA ATGAACTGCT TAACAACAAA CTGGAGTTTG AACATTTCCG TCAGTTTCTT
    GAGGCTCATT CTTCAAGCAT GGACCTTATG TGCTGGACAG ACATTGAACA GTTCCGAAGA
    ATAACTTACA GAGATCGAAA GCAAAGGGAG GCAAAATCTA TATATATAAA AAACAAATAC
    CTCAATAAAA AGTATTTCTT TGGGCCCAAC AGTCCAGCTT CTGTGTATCA ACAGGACCAG
    ATAATGAATT TAAGTGGTGG CTGGGGAAAG ATTCTCCATG AGCAACTTGA TGCAGCTGTT
    CTAATCGAAA TTCAGAAGCA TGTCCTAAAT AGACTAGAAA ATGTTTGGTT GCCGTTGTTT
    CTTGCAAGTG AACAGTTTGC GGCACGTCAA AAGATAAAGG TACAGATAAA AGACATAGCT
    GAAGAGCTTT TACTACAAAA GCGTGAAAGG AAAATTGGAG TCTGGAAGCC TGTGGAGAGT
    AAGTGGATAT CTTCGTCTTG TGAAATCATT GCTTTCCGTA AAGCTTTATT GAATCCAGTT
    ACTGCAAGAC AATTTCAACG CTTTGTGGCT CTAAAAGGAG ATTTATTGGA AAATGGGGTG
    CTCTTTTGGC AAGAAGTACA AAAATATAAG GACTTGTGCC ATTCTCATTG TGATGAGTCT
    ATCATACACA AGAAGATTAT GACTATTATC AACTGCTTTA TTAATTCCAG CATCCCCCCA
    GCTTTACAAA TTGACATTCC AGTAGAGCAA GCCCAGAAGA TTATTGAACA CAGGAAGGAG
    TTGGGACCAT ATGTATTCAG AGAGGCACAG ATGACAATTT TTGGAGTTCT GTTTAAATTT
    TGGCCTCAGT TCTGTGAGTT TAGGAAGAAC TTAACTGATG AAAAAATTAT GAGTGTTTTG
    GAGAGAAGAC AAGAATATAG TAAGCAGAAA AAGAAAATGG CAGTCATAGA AGATGAAAAA
    TTTGTAAAGG ATGGAATCAA ACAATATTCG AGTACTTCAG GATCTGTTAT CAAAGCAGCT
    GCTTTAACTA GTGACCCAGG TTTAGGCATG CAAGCGTACG GCCGACAGCC AACCTGGTGC
    TACTCAAAGT ACATAGAAGC CTTAGAACAG GAAAGGATTC TTCTTAAAAT TCAAGAGGAA
    CTAGAAAGAA AGTTGTTTGC AGCCACACCA TCTTTTACAA ATTTCAAGTC TACTGCTTCC
    ACTCTGTCTT TGAAAAAGAC TGTGTCCTCC CACAACATCC AGAGAATTCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26089
    Clone ID Related Accession (Same CDS sequence) XM_015466374.1 , XM_002692801.4 , XM_002692801.5
    Accession Version XM_002692801.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product regulator of G-protein signaling 22 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002692801.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGTC TATGGAGTTT TTGAAGTAGT TAACAATGCT
    CCACAACTTC TGGAAAAGCA GTTGAAAAAA ATTGTACTAA ACCAGCGACC TCGAAATCCT
    ATCTATGATG TTGTAAGGAA AGGAAAGAGT GATGTTAAAC CTGTTCAAGT GAACGCCCCT
    CACGAAGATG AGACCATTAA TGTCAACTAC AATATCATGT GTCTCAATCG TGAACAAGGT
    ATTCACTGGG TCAAAAAAGA AAGACTTCCA ACTTTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGGTTAG CCAAATTGAT TTCACAAGTA AGATGGAGTG AATCAGGCAT GAATATCATA
    TTAGGTACAG AGTTTTCACC CTGGGTCCTG AAAAAACCAC CCAGTCCACC ACCTCCTCCT
    GTTGAAGAAG ATGATGTTAT AATTATGAAG AAGTTCTACA TTTCTCTGGG CGAGGCCTCC
    TATACTCAAA CAAAAGATTG GTTTGCCTTA GCAAAACAAT GTCAGCAAAC AGTACCAACC
    TTTTCACTAC CATCTTGTGT ATTCCACAAC AAAATTGAAT CACCAGTTGT TTCATCTGCT
    TCTGAGAGTT TTATCTTCGA TGATGGAGTT CACCCCAGGA CGAAAAAGGG CCTATTAAAA
    ACCACTAGAT TAACCTCCGA ATTTGAAGAA GATGGGTCTG TATCTCTACA GGACACTCCT
    TCTCAAGCTC TTCTGAGGAT ATACCTTGAA AAGCAACAGA ATGTTGATGG AAGTCTGACA
    CTGCACTTCT CAACATGTGA CGAGTTTTTA AGATCTTATG TCTCCTTTAT CTTGAGAGTG
    GCGATTCATC AAATTCTTGG AGCAACACTT AGAGAAAGCC CAGATTACGT AAATTTCAGA
    AACGTAACAG AAGTGGTATT TGATGACATT GGGGGGCCTA CCTCTGGCAA GAATGTTTTG
    TTCAATGAAA TGCTAAGGGA AGAGTCGGGA GACATGTTAG AACCAGCAGT TTCAAAGCCT
    GACAGCATTG GACTCGACAG CAGGGCTGAT TGGTGTATTT CTCACAAAAC TTATGACATT
    GGTAATAGAA AGGAGTTTGA AAGATTTAAG AAATTTTTAA AAGGTACCTT AGGGGAGAAA
    TATTGGTGGC TATGGATGGA TATTGAGAGG TTAAAAGTTC TTAAGAATCC TGGAAGACAT
    CAAAGACATA TTGAGAAGAT GAAAAAATGC TTTCTAGTGA GCAGTGGAGA TTACTACCTT
    TCTGCAGAAG TCTTATCAAA ATTTAAACTA CTAGATGGAT CTCAGTGGAA TGAAGAACAC
    TTAAAAAAAA TTCAGACTGA GGTTTTAAAG CCCTTACTTC TTTATTGGGC ACCAAGATTC
    TGTGTTACTC ACTCATCCTC AACAAAACAT GCTAGTGCCG AGCTGAAATT CTGGCACCTT
    CGTCAGGAGC AGCCGAGGAA GGATGTTGAC CCTTTCCCAC AAATGGCAAC CCTCTTGCCG
    TTAAGACCCA AATCTTGCAT TCCACAGCTA CCTGAGATGC AGAAAGAAGA ATCCAGTTTA
    CTACAACTTC CTAAATCTCC CAAGAAATCA CCTAGAGTAA AGACAGCAGT TTATAAACCT
    TGGAAGAGTG AGTCTTTGAA TCCAGTTTCT TCTAAGGATG ATGTGATTGA GAAAGGGTCA
    AGGCACATGT CAGAAAGCAG CAAAGTTATT CGTTTAACAT CTTTTACTGA CATTTCTGAA
    TGTCTGAAGC CCCAGCTGGA TAGAAGATAT ACCTATACAG AAGAACCTTT GATTAAAACC
    ATGGGTGATG GTACCTTGGG AGGATTTGAC ATGGAGAATT TGTTGCAATC TTTGTATGTA
    GAAAACAGAG CTGGATTCTT CTTTACTAAA TTCTGCGAGA ACTCAGGGAA CAAGTTGTGG
    AAGAACTGTG TGTACTTTTG GTTTGACCTA CAAGCTTATC ATCAGCTTTT CTACCAAGAA
    ACCCTTCACC CTTTTAAAGT ATGCAAGCAA GCTCAATATC TTTTTGCTAC ATACATTGCT
    CCTTCAGCCA CTCTTGACAT TGGACTTCAA CAGGAAAAGA AAAGAGAAAT TTATATGAAA
    ATACAACCAC CATTTGAAGA CCTTTTTGAT GCAGCAGAAG AATATATCCT CCTCCTCCTT
    CTTGAGCCTT GGACAAAGAT GATAAAATCA GACCAAGTTG CTTACAGAAA GGTGGAGCTG
    GTGGAAGAAA CTCGACAGCT GGATTCCACA TACTTGAGAA AGCTACAGGC TTTGCATAAA
    GAGACAGTTT TCAAGAAAGC TGAGGACACT ACTTCTGAAA TTGGAACTGG TATTTCACCA
    CTTCCTCATG TCTCTAAAAA CACAGAATAC TGGAATATGG TTCCTGAAGA ATATAGGCAT
    TTTAATTTTA ATGAACTGCT TAACAACAAA CTGGAGTTTG AACATTTCCG TCAGTTTCTT
    GAGGCTCATT CTTCAAGCAT GGACCTTATG TGCTGGACAG ACATTGAACA GTTCCGAAGA
    ATAACTTACA GAGATCGAAA GCAAAGGGAG GCAAAATCTA TATATATAAA AAACAAATAC
    CTCAATAAAA AGTATTTCTT TGGGCCCAAC AGTCCAGCTT CTGTGTATCA ACAGGACCAG
    ATAATGAATT TAAGTGGTGG CTGGGGAAAG ATTCTCCATG AGCAACTTGA TGCAGCTGTT
    CTAATCGAAA TTCAGAAGCA TGTCCTAAAT AGACTAGAAA ATGTTTGGTT GCCGTTGTTT
    CTTGCAAGTG AACAGTTTGC GGCACGTCAA AAGATAAAGG TACAGATAAA AGACATAGCT
    GAAGAGCTTT TACTACAAAA GCGTGAAAGG AAAATTGGAG TCTGGAAGCC TGTGGAGAGT
    AAGTGGATAT CTTCGTCTTG TGAAATCATT GCTTTCCGTA AAGCTTTATT GAATCCAGTT
    ACTGCAAGAC AATTTCAACG CTTTGTGGCT CTAAAAGGAG ATTTATTGGA AAATGGGGTG
    CTCTTTTGGC AAGAAGTACA AAAATATAAG GACTTGTGCC ATTCTCATTG TGATGAGTCT
    ATCATACACA AGAAGATTAT GACTATTATC AACTGCTTTA TTAATTCCAG CATCCCCCCA
    GCTTTACAAA TTGACATTCC AGTAGAGCAA GCCCAGAAGA TTATTGAACA CAGGAAGGAG
    TTGGGACCAT ATGTATTCAG AGAGGCACAG ATGACAATTT TTGGAGTTCT GTTTAAATTT
    TGGCCTCAGT TCTGTGAGTT TAGGAAGAAC TTAACTGATG AAAAAATTAT GAGTGTTTTG
    GAGAGAAGAC AAGAATATAG TAAGCAGAAA AAGAAAATGG CAGTCATAGA AGATGAAAAA
    TTTGTAAAGG ATGGAATCAA ACAATATTCG AGTACTTCAG GATCTGTTAT CAAAGCAGCT
    GCTTTAACTA GTGACCCAGG TTTAGGCATG CAAGCGTACG GCCGACAGCC AACCTGGTGC
    TACTCAAAGT ACATAGAAGC CTTAGAACAG GAAAGGATTC TTCTTAAAAT TCAAGAGGAA
    CTAGAAAGAA AGTTGTTTGC AGCCACACCA TCTTTTACAA ATTTCAAGTC TACTGCTTCC
    ACTCTGTCTT TGAAAAAGAC TGTGTCCTCC CACAACATCC AGAGAATTCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002692847.2
    CDS77..3850
    Translation

    Target ORF information:

    RefSeq Version XM_002692801.5
    Organism Bos taurus(cattle)
    Definition Bos taurus regulator of G protein signaling 22 (RGS22), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002692801.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCCCGAGA GGCGGCTTAC CGTCGAGCCA CCAACTATTA CAGAAGAAGG ATTTGAAGAT 
    TCTTTGGCGA CAGATGATTT CCTTGTGGAC TACTTTAATG AATTCCTAAG CCTTCCAACC
    TTTTCAGAGG CAGTTAGATT TAATGTGGTC TATGGAGTTT TTGAAGTAGT TAACAATGCT
    CCACAACTTC TGGAAAAGCA GTTGAAAAAA ATTGTACTAA ACCAGCGACC TCGAAATCCT
    ATCTATGATG TTGTAAGGAA AGGAAAGAGT GATGTTAAAC CTGTTCAAGT GAACGCCCCT
    CACGAAGATG AGACCATTAA TGTCAACTAC AATATCATGT GTCTCAATCG TGAACAAGGT
    ATTCACTGGG TCAAAAAAGA AAGACTTCCA ACTTTTCTGG AAAGTGATTG TTACTTTGAA
    TACAGGTTAG CCAAATTGAT TTCACAAGTA AGATGGAGTG AATCAGGCAT GAATATCATA
    TTAGGTACAG AGTTTTCACC CTGGGTCCTG AAAAAACCAC CCAGTCCACC ACCTCCTCCT
    GTTGAAGAAG ATGATGTTAT AATTATGAAG AAGTTCTACA TTTCTCTGGG CGAGGCCTCC
    TATACTCAAA CAAAAGATTG GTTTGCCTTA GCAAAACAAT GTCAGCAAAC AGTACCAACC
    TTTTCACTAC CATCTTGTGT ATTCCACAAC AAAATTGAAT CACCAGTTGT TTCATCTGCT
    TCTGAGAGTT TTATCTTCGA TGATGGAGTT CACCCCAGGA CGAAAAAGGG CCTATTAAAA
    ACCACTAGAT TAACCTCCGA ATTTGAAGAA GATGGGTCTG TATCTCTACA GGACACTCCT
    TCTCAAGCTC TTCTGAGGAT ATACCTTGAA AAGCAACAGA ATGTTGATGG AAGTCTGACA
    CTGCACTTCT CAACATGTGA CGAGTTTTTA AGATCTTATG TCTCCTTTAT CTTGAGAGTG
    GCGATTCATC AAATTCTTGG AGCAACACTT AGAGAAAGCC CAGATTACGT AAATTTCAGA
    AACGTAACAG AAGTGGTATT TGATGACATT GGGGGGCCTA CCTCTGGCAA GAATGTTTTG
    TTCAATGAAA TGCTAAGGGA AGAGTCGGGA GACATGTTAG AACCAGCAGT TTCAAAGCCT
    GACAGCATTG GACTCGACAG CAGGGCTGAT TGGTGTATTT CTCACAAAAC TTATGACATT
    GGTAATAGAA AGGAGTTTGA AAGATTTAAG AAATTTTTAA AAGGTACCTT AGGGGAGAAA
    TATTGGTGGC TATGGATGGA TATTGAGAGG TTAAAAGTTC TTAAGAATCC TGGAAGACAT
    CAAAGACATA TTGAGAAGAT GAAAAAATGC TTTCTAGTGA GCAGTGGAGA TTACTACCTT
    TCTGCAGAAG TCTTATCAAA ATTTAAACTA CTAGATGGAT CTCAGTGGAA TGAAGAACAC
    TTAAAAAAAA TTCAGACTGA GGTTTTAAAG CCCTTACTTC TTTATTGGGC ACCAAGATTC
    TGTGTTACTC ACTCATCCTC AACAAAACAT GCTAGTGCCG AGCTGAAATT CTGGCACCTT
    CGTCAGGAGC AGCCGAGGAA GGATGTTGAC CCTTTCCCAC AAATGGCAAC CCTCTTGCCG
    TTAAGACCCA AATCTTGCAT TCCACAGCTA CCTGAGATGC AGAAAGAAGA ATCCAGTTTA
    CTACAACTTC CTAAATCTCC CAAGAAATCA CCTAGAGTAA AGACAGCAGT TTATAAACCT
    TGGAAGAGTG AGTCTTTGAA TCCAGTTTCT TCTAAGGATG ATGTGATTGA GAAAGGGTCA
    AGGCACATGT CAGAAAGCAG CAAAGTTATT CGTTTAACAT CTTTTACTGA CATTTCTGAA
    TGTCTGAAGC CCCAGCTGGA TAGAAGATAT ACCTATACAG AAGAACCTTT GATTAAAACC
    ATGGGTGATG GTACCTTGGG AGGATTTGAC ATGGAGAATT TGTTGCAATC TTTGTATGTA
    GAAAACAGAG CTGGATTCTT CTTTACTAAA TTCTGCGAGA ACTCAGGGAA CAAGTTGTGG
    AAGAACTGTG TGTACTTTTG GTTTGACCTA CAAGCTTATC ATCAGCTTTT CTACCAAGAA
    ACCCTTCACC CTTTTAAAGT ATGCAAGCAA GCTCAATATC TTTTTGCTAC ATACATTGCT
    CCTTCAGCCA CTCTTGACAT TGGACTTCAA CAGGAAAAGA AAAGAGAAAT TTATATGAAA
    ATACAACCAC CATTTGAAGA CCTTTTTGAT GCAGCAGAAG AATATATCCT CCTCCTCCTT
    CTTGAGCCTT GGACAAAGAT GATAAAATCA GACCAAGTTG CTTACAGAAA GGTGGAGCTG
    GTGGAAGAAA CTCGACAGCT GGATTCCACA TACTTGAGAA AGCTACAGGC TTTGCATAAA
    GAGACAGTTT TCAAGAAAGC TGAGGACACT ACTTCTGAAA TTGGAACTGG TATTTCACCA
    CTTCCTCATG TCTCTAAAAA CACAGAATAC TGGAATATGG TTCCTGAAGA ATATAGGCAT
    TTTAATTTTA ATGAACTGCT TAACAACAAA CTGGAGTTTG AACATTTCCG TCAGTTTCTT
    GAGGCTCATT CTTCAAGCAT GGACCTTATG TGCTGGACAG ACATTGAACA GTTCCGAAGA
    ATAACTTACA GAGATCGAAA GCAAAGGGAG GCAAAATCTA TATATATAAA AAACAAATAC
    CTCAATAAAA AGTATTTCTT TGGGCCCAAC AGTCCAGCTT CTGTGTATCA ACAGGACCAG
    ATAATGAATT TAAGTGGTGG CTGGGGAAAG ATTCTCCATG AGCAACTTGA TGCAGCTGTT
    CTAATCGAAA TTCAGAAGCA TGTCCTAAAT AGACTAGAAA ATGTTTGGTT GCCGTTGTTT
    CTTGCAAGTG AACAGTTTGC GGCACGTCAA AAGATAAAGG TACAGATAAA AGACATAGCT
    GAAGAGCTTT TACTACAAAA GCGTGAAAGG AAAATTGGAG TCTGGAAGCC TGTGGAGAGT
    AAGTGGATAT CTTCGTCTTG TGAAATCATT GCTTTCCGTA AAGCTTTATT GAATCCAGTT
    ACTGCAAGAC AATTTCAACG CTTTGTGGCT CTAAAAGGAG ATTTATTGGA AAATGGGGTG
    CTCTTTTGGC AAGAAGTACA AAAATATAAG GACTTGTGCC ATTCTCATTG TGATGAGTCT
    ATCATACACA AGAAGATTAT GACTATTATC AACTGCTTTA TTAATTCCAG CATCCCCCCA
    GCTTTACAAA TTGACATTCC AGTAGAGCAA GCCCAGAAGA TTATTGAACA CAGGAAGGAG
    TTGGGACCAT ATGTATTCAG AGAGGCACAG ATGACAATTT TTGGAGTTCT GTTTAAATTT
    TGGCCTCAGT TCTGTGAGTT TAGGAAGAAC TTAACTGATG AAAAAATTAT GAGTGTTTTG
    GAGAGAAGAC AAGAATATAG TAAGCAGAAA AAGAAAATGG CAGTCATAGA AGATGAAAAA
    TTTGTAAAGG ATGGAATCAA ACAATATTCG AGTACTTCAG GATCTGTTAT CAAAGCAGCT
    GCTTTAACTA GTGACCCAGG TTTAGGCATG CAAGCGTACG GCCGACAGCC AACCTGGTGC
    TACTCAAAGT ACATAGAAGC CTTAGAACAG GAAAGGATTC TTCTTAAAAT TCAAGAGGAA
    CTAGAAAGAA AGTTGTTTGC AGCCACACCA TCTTTTACAA ATTTCAAGTC TACTGCTTCC
    ACTCTGTCTT TGAAAAAGAC TGTGTCCTCC CACAACATCC AGAGAATTCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.