TUT1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TUT1
Entrez Gene ID	616339
Full Name	terminal uridylyl transferase 1, U6 snRNA-specific
Synonyms	RBM21
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001080322.1	NP_001073791.1	speckle targeted PIP5K1A-regulated poly(A) polymerase
XM_024987333.1	XP_024843101.1	speckle targeted PIP5K1A-regulated poly(A) polymerase isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TUT1	XP_005631681.1
Rattus norvegicus (Norway rat)	Tut1	NP_001029073.1
Homo sapiens (human)	TUT1	NP_073741.2
Xenopus tropicalis (tropical clawed frog)	tut1	XP_002941502.2
Bos taurus (cattle)	TUT1	NP_001073791.1
Danio rerio (zebrafish)	tut1	NP_001025359.1
Mus musculus (house mouse)	Tut1	NP_932110.1
Pan troglodytes (chimpanzee)	TUT1	XP_508491.3

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TUT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TUT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa42193	NM_001080322.1 Latest version!	Bos taurus terminal uridylyl transferase 1, U6 snRNA-specific (TUT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa56013	XM_024987333.1 Latest version!	Bos taurus terminal uridylyl transferase 1, U6 snRNA-specific (TUT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TUT1 ( NM_001080322.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001073791.1 Bos taurus terminal uridylyl transferase 1, U6 snRNA-specific (TUT1), mRNA.

Price & Availability↑

CloneID	OBa42193
Clone ID Related Accession (Same CDS sequence)	NM_001080322.1
Accession Version	NM_001080322.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Bos taurus(cattle)
Product	speckle targeted PIP5K1A-regulated poly(A) polymerase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BT025417.1. On Jan 16, 2007 this sequence version replaced XM_868335.2. ##Evidence-Data-START## Transcript exon combination :: BT025417.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGCAG TGGATTCAGA TATCGAGCCG CTGCCTCGTG GGGGTTTCCG CTGCTGCCTC 
TGCCACATCA CTACAGCCAA CCAACCCAGC CTTGATGCCC ACCTGGGAGG CCGGAAGCAC 
CGGCACCTGG TGGAACTACG AGCTACTCGA AAGGCCCAAG GACTTCGAAG CGTGTTTGTC 
AGTGGCTTTC CCCGGGATGT GGATTCTACT CAGCTCTCTG AGTACTTCCA GGCATTTGGA 
CCTGTGGCCA GTGTTGTCAT GGACAAGGAC AAGGGTGTGT TTGCCATCGT GGAGATGGGG 
GACCTGGGTG CCCGGGAAGC TGTTTTGTCA CAGCCCCAGC ACAGCCTGGG AGGACGTCGC 
CTGAGGGTCC GGCCACGGGA GCAGATAGAG TTCCAGAGCC CAGCCTCCAG GTCCCCCAAA 
AGAGTGGCCC CCGACAGTCA CCAGCTGATC AAAGCACTCG CCGAGGCCCC GGACGTGGAG 
GCACAAATGG TGAAGCTCGT GGGGCTGAGG GAGCTCTCCG AGGCTGAGCG GCAGCTTCGG 
AGCCTTGTGG TGGCCCTAAT GCAGGAAGTC TTCGCAGAGT TCTTCCCTGG CTGTGTGGTC 
CATCCTTTTG GCTCCTCCAT AAACAGCTTT GACGTCCATG GCTGTGATCT CGACCTCTTC 
CTGGATCTGG GGGACTTGGA CGAGCCCCAG CCAGCCCCAA AGGCTCCAGA ATCTCCCTCC 
TTGGACTCGG CCCTGGCATC CCCACTGGAT CCTCAAGCCC TGGCCTGCAC CCCAGCCTCC 
CCTCCAGACT CACAGCCTCC AGCTTCTCCT CAAGACTCGG AAGCCCTGGA CTTTGAAGCC 
CCTTCCTCCT CACTGGCACC TCGGACTCCA GACTCTGCTT TGGCCTCTGA GACCCTCGCC 
TCCCCCCGGT CCCTGCCTCC AGCCTCGCCG CTGCAGGAGG ACCAGGGAGA CGGGGACCAG 
GGGAAGGCGG TGGAACTGGC AGAGGCCCTG AAGGGGGAGA AAGCAGAAGG GGGAGCCATG 
CTGGAGCTGG TGGGATCCAT CCTTCGGGGC TGTGTTCCTG GAGTGTACCG AGTCCAAACC 
GTGCCCTCTG CCCGGTGCCC TGTGGTCAAG TTCTGCCATC GGCCTTCAGG TCTCCATGGT 
GACATCTCCC TCAGTAACCG GCTGGCCCTG CACAACTCCC GCTTCCTGAG TCTCTGCTCT 
GAGCTGGATG GGCGAGTACG GCCCCTTGTG TACACTCTGC GCTGCTGGGC TCAGGGTCGA 
GGGCTGTCAG GGAGTGGCCC ACTTCTCAAT AACTACGCCC TGACCTTGCT CGTGATCTAT 
TTTCTTCAGA CCAGGGACCC TCCTGTGTTG CCCACTGTGT CCCAGCTCAC CCAGAAAGCA 
GGCGAACAGG TGGAGGTTGA TGGCTGGGAC TGTAGTTTCC CCAGAGATGC CTCAAGACTG 
GAGCCCAGCA CCAATAAGGA GCCTCTGAGT TCCCTGCTGG CCCAGTTCTT CTCTTGCGTC 
TCTTGTTGGG ATCTTCGTGG CTCCCTGCTG TCCCTGCGGG AGGGTCAGGC ACTATCTGTG 
GCAGGGGGCC TGCCCTCTAA TCTCTCAGAG GGCCTGCGCC TCGGCCCCAT GAATCTCCAG 
GACCCCTTTG ACCTGAGTCA CAATGTGGCA GCCAACGTGA CCAGCCGGGT GGCCGGGAGG 
CTACAGAACT GTTGCCGAGC GGCAGCCAAT TACTGTCGAA GCCTCCAGTA CCAGCGGCGG 
TCTTCCCGGG GTCGGGACTG GGGCCTGCTT CCCCTTCTGC AGCCCAGCTC CCCCAGCTCC 
ATCCTCTCTG CAACACCTAT CCCCTTACCC CCTGCTTCCT TCACCCAGCT CACTGCTGTG 
CTGGCCCAGG TGTTAAGGGA AGCCTTAGGG TGCCATATAG AACAGGGAAC CAAGAGACTG 
CGGTCAGAGG GAGGTGGCCC TGGGGAGCCC CCCCAGGGAG GGACAAGCAA GAGAGCAAAA 
CTAGATGGAC AGAAAAAAAG CTGTGAGGAG GGGCCAGAGG AGCAGCAGGG ATGTGCAGGG 
GAGCATGGTG AAGATGGCGT GGAAGAGATG GTCATAGAGG TTGGAGAGTC GGTGCAGGAC 
TGGGTGATGC GGAGCCCTGG GCAGCTGGGG GAGCTGCCCC TGATGACCGG AAAGCATCTA 
GCCACCAGAG AAGAGGGGCA GTCAGGCACC GCAGCGCTGG CCAAGCAGGG GCCCAGAGGA 
CCTGAGGCAG CCTGCGAAGG GTCTCAGGCC GAGGCCGAGA AGCGGGTGTC GCTCACAGTG 
AGCTGGCGCT GCGCCTTGTG GCACCGTGTG TGGCAGGGGC GGAGGCGTGC CCGGAGACGC 
CTGCAGCAAC AAATCAAGGA AGGAGGTGGA TCTGGTGCTG GCTCGGGAGC AGAGTGGCTG 
GCAACTGAGG CTCAGGTCAC CCGGGAGCTG CGGGGACTGA GCAGTACTGA ACAGAGGCCA 
GAGGCTGAGC CACTCCTGAC CTTCGTGGCA TCCACCTCCC AGGCTGACCA GAGTCTCACT 
GTGACCCCAC TCCAGGATTC TCAAGGCCTG TTCCCTGATC TCCATCATTT CTTACAGGTT 
TTCCTCCCTC AAGCACTTCG AAACCTGCTT AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073791.1
CDS	34..2649
Translation	MAAVDSDIEPLPRGGFRCCLCHITTANQPSLDAHLGGRKHRHLVELRATRKAQGLRSVFVSGFPRDVDSTQLSEYFQAFGPVASVVMDKDKGVFAIVEMGDLGAREAVLSQPQHSLGGRRLRVRPREQIEFQSPASRSPKRVAPDSHQLIKALAEAPDVEAQMVKLVGLRELSEAERQLRSLVVALMQEVFAEFFPGCVVHPFGSSINSFDVHGCDLDLFLDLGDLDEPQPAPKAPESPSLDSALASPLDPQALACTPASPPDSQPPASPQDSEALDFEAPSSSLAPRTPDSALASETLASPRSLPPASPLQEDQGDGDQGKAVELAEALKGEKAEGGAMLELVGSILRGCVPGVYRVQTVPSARCPVVKFCHRPSGLHGDISLSNRLALHNSRFLSLCSELDGRVRPLVYTLRCWAQGRGLSGSGPLLNNYALTLLVIYFLQTRDPPVLPTVSQLTQKAGEQVEVDGWDCSFPRDASRLEPSTNKEPLSSLLAQFFSCVSCWDLRGSLLSLREGQALSVAGGLPSNLSEGLRLGPMNLQDPFDLSHNVAANVTSRVAGRLQNCCRAAANYCRSLQYQRRSSRGRDWGLLPLLQPSSPSSILSATPIPLPPASFTQLTAVLAQVLREALGCHIEQGTKRLRSEGGGPGEPPQGGTSKRAKLDGQKKSCEEGPEEQQGCAGEHGEDGVEEMVIEVGESVQDWVMRSPGQLGELPLMTGKHLATREEGQSGTAALAKQGPRGPEAACEGSQAEAEKRVSLTVSWRCALWHRVWQGRRRARRRLQQQIKEGGGSGAGSGAEWLATEAQVTRELRGLSSTEQRPEAEPLLTFVASTSQADQSLTVTPLQDSQGLFPDLHHFLQVFLPQALRNLLK

Target ORF information:

RefSeq Version	NM_001080322.1
Organism	Bos taurus(cattle)
Definition	Bos taurus terminal uridylyl transferase 1, U6 snRNA-specific (TUT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080322.1

ORF Insert Sequence:

ATGGCTGCAG TGGATTCAGA TATCGAGCCG CTGCCTCGTG GGGGTTTCCG CTGCTGCCTC 
TGCCACATCA CTACAGCCAA CCAACCCAGC CTTGATGCCC ACCTGGGAGG CCGGAAGCAC 
CGGCACCTGG TGGAACTACG AGCTACTCGA AAGGCCCAAG GACTTCGAAG CGTGTTTGTC 
AGTGGCTTTC CCCGGGATGT GGATTCTACT CAGCTCTCTG AGTACTTCCA GGCATTTGGA 
CCTGTGGCCA GTGTTGTCAT GGACAAGGAC AAGGGTGTGT TTGCCATCGT GGAGATGGGG 
GACCTGGGTG CCCGGGAAGC TGTTTTGTCA CAGCCCCAGC ACAGCCTGGG AGGACGTCGC 
CTGAGGGTCC GGCCACGGGA GCAGATAGAG TTCCAGAGCC CAGCCTCCAG GTCCCCCAAA 
AGAGTGGCCC CCGACAGTCA CCAGCTGATC AAAGCACTCG CCGAGGCCCC GGACGTGGAG 
GCACAAATGG TGAAGCTCGT GGGGCTGAGG GAGCTCTCCG AGGCTGAGCG GCAGCTTCGG 
AGCCTTGTGG TGGCCCTAAT GCAGGAAGTC TTCGCAGAGT TCTTCCCTGG CTGTGTGGTC 
CATCCTTTTG GCTCCTCCAT AAACAGCTTT GACGTCCATG GCTGTGATCT CGACCTCTTC 
CTGGATCTGG GGGACTTGGA CGAGCCCCAG CCAGCCCCAA AGGCTCCAGA ATCTCCCTCC 
TTGGACTCGG CCCTGGCATC CCCACTGGAT CCTCAAGCCC TGGCCTGCAC CCCAGCCTCC 
CCTCCAGACT CACAGCCTCC AGCTTCTCCT CAAGACTCGG AAGCCCTGGA CTTTGAAGCC 
CCTTCCTCCT CACTGGCACC TCGGACTCCA GACTCTGCTT TGGCCTCTGA GACCCTCGCC 
TCCCCCCGGT CCCTGCCTCC AGCCTCGCCG CTGCAGGAGG ACCAGGGAGA CGGGGACCAG 
GGGAAGGCGG TGGAACTGGC AGAGGCCCTG AAGGGGGAGA AAGCAGAAGG GGGAGCCATG 
CTGGAGCTGG TGGGATCCAT CCTTCGGGGC TGTGTTCCTG GAGTGTACCG AGTCCAAACC 
GTGCCCTCTG CCCGGTGCCC TGTGGTCAAG TTCTGCCATC GGCCTTCAGG TCTCCATGGT 
GACATCTCCC TCAGTAACCG GCTGGCCCTG CACAACTCCC GCTTCCTGAG TCTCTGCTCT 
GAGCTGGATG GGCGAGTACG GCCCCTTGTG TACACTCTGC GCTGCTGGGC TCAGGGTCGA 
GGGCTGTCAG GGAGTGGCCC ACTTCTCAAT AACTACGCCC TGACCTTGCT CGTGATCTAT 
TTTCTTCAGA CCAGGGACCC TCCTGTGTTG CCCACTGTGT CCCAGCTCAC CCAGAAAGCA 
GGCGAACAGG TGGAGGTTGA TGGCTGGGAC TGTAGTTTCC CCAGAGATGC CTCAAGACTG 
GAGCCCAGCA CCAATAAGGA GCCTCTGAGT TCCCTGCTGG CCCAGTTCTT CTCTTGCGTC 
TCTTGTTGGG ATCTTCGTGG CTCCCTGCTG TCCCTGCGGG AGGGTCAGGC ACTATCTGTG 
GCAGGGGGCC TGCCCTCTAA TCTCTCAGAG GGCCTGCGCC TCGGCCCCAT GAATCTCCAG 
GACCCCTTTG ACCTGAGTCA CAATGTGGCA GCCAACGTGA CCAGCCGGGT GGCCGGGAGG 
CTACAGAACT GTTGCCGAGC GGCAGCCAAT TACTGTCGAA GCCTCCAGTA CCAGCGGCGG 
TCTTCCCGGG GTCGGGACTG GGGCCTGCTT CCCCTTCTGC AGCCCAGCTC CCCCAGCTCC 
ATCCTCTCTG CAACACCTAT CCCCTTACCC CCTGCTTCCT TCACCCAGCT CACTGCTGTG 
CTGGCCCAGG TGTTAAGGGA AGCCTTAGGG TGCCATATAG AACAGGGAAC CAAGAGACTG 
CGGTCAGAGG GAGGTGGCCC TGGGGAGCCC CCCCAGGGAG GGACAAGCAA GAGAGCAAAA 
CTAGATGGAC AGAAAAAAAG CTGTGAGGAG GGGCCAGAGG AGCAGCAGGG ATGTGCAGGG 
GAGCATGGTG AAGATGGCGT GGAAGAGATG GTCATAGAGG TTGGAGAGTC GGTGCAGGAC 
TGGGTGATGC GGAGCCCTGG GCAGCTGGGG GAGCTGCCCC TGATGACCGG AAAGCATCTA 
GCCACCAGAG AAGAGGGGCA GTCAGGCACC GCAGCGCTGG CCAAGCAGGG GCCCAGAGGA 
CCTGAGGCAG CCTGCGAAGG GTCTCAGGCC GAGGCCGAGA AGCGGGTGTC GCTCACAGTG 
AGCTGGCGCT GCGCCTTGTG GCACCGTGTG TGGCAGGGGC GGAGGCGTGC CCGGAGACGC 
CTGCAGCAAC AAATCAAGGA AGGAGGTGGA TCTGGTGCTG GCTCGGGAGC AGAGTGGCTG 
GCAACTGAGG CTCAGGTCAC CCGGGAGCTG CGGGGACTGA GCAGTACTGA ACAGAGGCCA 
GAGGCTGAGC CACTCCTGAC CTTCGTGGCA TCCACCTCCC AGGCTGACCA GAGTCTCACT 
GTGACCCCAC TCCAGGATTC TCAAGGCCTG TTCCCTGATC TCCATCATTT CTTACAGGTT 
TTCCTCCCTC AAGCACTTCG AAACCTGCTT AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TUT1 ( XM_024987333.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843101.1 Bos taurus terminal uridylyl transferase 1, U6 snRNA-specific (TUT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa56013
Clone ID Related Accession (Same CDS sequence)	XM_024987333.1
Accession Version	XM_024987333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	speckle targeted PIP5K1A-regulated poly(A) polymerase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACC TGGGTGCCCG GGAAGCTGTT TTGTCACAGC CCCAGCACAG CCTGGGAGGA 
CGTCGCCTGA GGGTCCGGCC ACGGGAGCAG AAAGAGTTCC AGAGCCCAGC CTCCAGGTCC 
CCCAAAAGAG TGGCCCCCGA CAGTCACCAG CTGATCAAAG CACTCGCCGA GGCCCCGGAC 
GTGGAGGCAC AAATGGTGAA GCTCGTGGGG CTGAGGGAGC TCTCCGAGGC TGAGCGGCAG 
CTTCGGAGCC TTGTGGTGGC CCTAATGCAG GAAGTCTTCG CAGAGTTCTT CCCTGGCTGT 
GTGGTCCATC CTTTTGGCTC CTCCATAAAC AGCTTTGACG TCCATGGCTG TGATCTCGAC 
CTCTTCCTGG ATCTGGGGGA CTTGGACGAG CCCCAGCCAG CCCCAAAGGC TCCAGAATCT 
CCCTCCTTGG ACTCGGCCCT GGCATCCCCA CTGGATCCTC AAGCCCTGGC CTGCACCCCA 
GCCTCCCCTC CAGACTCACA GCCTCCAGCT TCTCCTCAAG ACTCGGAAGC CCTGGACTTT 
GAAGCCCCTT CCTCCTCACT GGCACCTCGG ACTCCAGACT CTGCTTTGGC CTCTGAGACC 
CTCGCCTCTC CCCAGTCCCT GCCTCCAGCC TCGCCGCTGC AGGAGGACCA GGGAGACGGG 
GACCAGGGGA AGGCGGTGGA ACTGGCAGAG GCCCTGAAGG GGGAGAAAGC AGAAGGGGGA 
GCCATGCTGG AGCTGGTGGG ATCCATCCTT CGGGGCTGTG TTCCTGGAGT GTACCGAGTC 
CAAACCGTGC CCTCTGCCCG GTGCCCTGTG GTCAAGTTCT GCCATCGGCC TTCAGGTCTC 
CATGGTGACA TCTCCCTCAG TAACCGGCTG GCCCTGCACA ACTCCCGCTT CCTGAGTCTC 
TGCTCTGAGC TGGATGGGCG AGTACGGCCC CTTGTGTACA CTCTGCGCTG CTGGGCTCAG 
GGTCGAGGGC TGTCAGGGAG TGGCCCACTT CTCAATAACT ACGCCCTGAC CTTGCTCGTG 
ATCTATTTTC TTCAGACCAG GGACCCTCCT GTGTTGCCCA CTGTGTCCCA GCTCACCCAG 
AAAGCAGGCG AACAGGTGGA GGTTGATGGC TGGGACTGTA GTTTCCCCAG AGATGCCTCA 
AGACTGGAGC CCAGCACCAA TAAGGAGCCT CTGAGTTCCC TGCTGGCCCA GTTCTTCTCT 
TGCGTCTCTT GTTGGGATCT TCGTGGCTCC CTGCTGTCCC TGCGGGAGGG TCAGGCACTA 
TCTGTGGCAG GGGGCCTGCC CTCTAATCTC TCAGAGGGCC TGCGCCTCGG CCCCATGAAT 
CTCCAGGACC CCTTTGACCT GAGTCACAAT GTGGCAGCCA ACGTGACCAG CCGGGTGGCC 
GGGAGGCTAC AGAACTGTTG CCGAGCGGCA GCCAATTACT GTCGAAGCCT CCAGTACCAG 
CGGCGGTCTT CCCGGGGTCG GGACTGGGGC CTGCTTCCCC TTCTGCAGCC CAGCTCCCCC 
AGCTCCATCC TCTCTGCAAC ACCTATCCCC TTACCCCCTG CTTCCTTCAC CCAGCTCACT 
GCTGTGCTGG CCCAGGTGTT AAGGGAAGCC TTAGGGTGCC ATATAGAACA GGGAACCAAG 
AGACTGCGGT CAGAGGGAGG TGGCCCTGGG GAGCCCCCCC AGGGAGGGAC AAGCAAGAGA 
GCAAAACTAG ATGGACAGAA AAAAAGCTGT GAGGAGGGGC CAGAGGAGCA GCAGGGATGT 
GCAGGGGAGC ATGGTGAAGA TGGCGTGGAA GAGATGGTCA TAGAGGTTGG AGAGTCGGTG 
CAGGACTGGG TGATGCGGAG CCCTGGGCAG CTGGGGGAGC TGCCCCTGAT GACCGGAAAG 
CATCTAGCCA CCAGAGAAGA GGGGCAGTCA GGCACCGCAG CGCTGGCCAA GCAGGGGCCC 
AGAGGACCTG AGGCAGCCTG CGAAGGGTCT CAGGCCGAGG CCGAGAAGCG GGTGTCGCTC 
ACAGTGAGCT GGCGCTGCGC CTTGTGGCAC CGTGTGTGGC AGGGGCGGAG GCGTGCCCGG 
AGACGCCTGC AGCAACAAAT CAAGGAAGGA GGTGGATCTG GTGCTGGCTC GGGAGCAGAG 
TGGCTGGCAA CTGAGGCTCA GGTCACCCGG GAGCTGCGGG GACTGAGCAG TACTGAACAG 
AGGCCAGAGG CTGAGCCACT CCTGACCTTC GTGGCATCCA CCTCCCAGGC TGACCAGAGT 
CTCACTGTGA CCCCACTCCA GGATTCTCAA GGCCTGTTCC CTGATCTCCA TCATTTCTTA 
CAGGTTTTCC TCCCTCAAGC ACTTCGAAAC CTGCTTAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843101.1
CDS	190..2511
Translation	MGDLGAREAVLSQPQHSLGGRRLRVRPREQKEFQSPASRSPKRVAPDSHQLIKALAEAPDVEAQMVKLVGLRELSEAERQLRSLVVALMQEVFAEFFPGCVVHPFGSSINSFDVHGCDLDLFLDLGDLDEPQPAPKAPESPSLDSALASPLDPQALACTPASPPDSQPPASPQDSEALDFEAPSSSLAPRTPDSALASETLASPQSLPPASPLQEDQGDGDQGKAVELAEALKGEKAEGGAMLELVGSILRGCVPGVYRVQTVPSARCPVVKFCHRPSGLHGDISLSNRLALHNSRFLSLCSELDGRVRPLVYTLRCWAQGRGLSGSGPLLNNYALTLLVIYFLQTRDPPVLPTVSQLTQKAGEQVEVDGWDCSFPRDASRLEPSTNKEPLSSLLAQFFSCVSCWDLRGSLLSLREGQALSVAGGLPSNLSEGLRLGPMNLQDPFDLSHNVAANVTSRVAGRLQNCCRAAANYCRSLQYQRRSSRGRDWGLLPLLQPSSPSSILSATPIPLPPASFTQLTAVLAQVLREALGCHIEQGTKRLRSEGGGPGEPPQGGTSKRAKLDGQKKSCEEGPEEQQGCAGEHGEDGVEEMVIEVGESVQDWVMRSPGQLGELPLMTGKHLATREEGQSGTAALAKQGPRGPEAACEGSQAEAEKRVSLTVSWRCALWHRVWQGRRRARRRLQQQIKEGGGSGAGSGAEWLATEAQVTRELRGLSSTEQRPEAEPLLTFVASTSQADQSLTVTPLQDSQGLFPDLHHFLQVFLPQALRNLLK

Target ORF information:

RefSeq Version	XM_024987333.1
Organism	Bos taurus(cattle)
Definition	Bos taurus terminal uridylyl transferase 1, U6 snRNA-specific (TUT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987333.1

ORF Insert Sequence:

ATGGGGGACC TGGGTGCCCG GGAAGCTGTT TTGTCACAGC CCCAGCACAG CCTGGGAGGA 
CGTCGCCTGA GGGTCCGGCC ACGGGAGCAG AAAGAGTTCC AGAGCCCAGC CTCCAGGTCC 
CCCAAAAGAG TGGCCCCCGA CAGTCACCAG CTGATCAAAG CACTCGCCGA GGCCCCGGAC 
GTGGAGGCAC AAATGGTGAA GCTCGTGGGG CTGAGGGAGC TCTCCGAGGC TGAGCGGCAG 
CTTCGGAGCC TTGTGGTGGC CCTAATGCAG GAAGTCTTCG CAGAGTTCTT CCCTGGCTGT 
GTGGTCCATC CTTTTGGCTC CTCCATAAAC AGCTTTGACG TCCATGGCTG TGATCTCGAC 
CTCTTCCTGG ATCTGGGGGA CTTGGACGAG CCCCAGCCAG CCCCAAAGGC TCCAGAATCT 
CCCTCCTTGG ACTCGGCCCT GGCATCCCCA CTGGATCCTC AAGCCCTGGC CTGCACCCCA 
GCCTCCCCTC CAGACTCACA GCCTCCAGCT TCTCCTCAAG ACTCGGAAGC CCTGGACTTT 
GAAGCCCCTT CCTCCTCACT GGCACCTCGG ACTCCAGACT CTGCTTTGGC CTCTGAGACC 
CTCGCCTCTC CCCAGTCCCT GCCTCCAGCC TCGCCGCTGC AGGAGGACCA GGGAGACGGG 
GACCAGGGGA AGGCGGTGGA ACTGGCAGAG GCCCTGAAGG GGGAGAAAGC AGAAGGGGGA 
GCCATGCTGG AGCTGGTGGG ATCCATCCTT CGGGGCTGTG TTCCTGGAGT GTACCGAGTC 
CAAACCGTGC CCTCTGCCCG GTGCCCTGTG GTCAAGTTCT GCCATCGGCC TTCAGGTCTC 
CATGGTGACA TCTCCCTCAG TAACCGGCTG GCCCTGCACA ACTCCCGCTT CCTGAGTCTC 
TGCTCTGAGC TGGATGGGCG AGTACGGCCC CTTGTGTACA CTCTGCGCTG CTGGGCTCAG 
GGTCGAGGGC TGTCAGGGAG TGGCCCACTT CTCAATAACT ACGCCCTGAC CTTGCTCGTG 
ATCTATTTTC TTCAGACCAG GGACCCTCCT GTGTTGCCCA CTGTGTCCCA GCTCACCCAG 
AAAGCAGGCG AACAGGTGGA GGTTGATGGC TGGGACTGTA GTTTCCCCAG AGATGCCTCA 
AGACTGGAGC CCAGCACCAA TAAGGAGCCT CTGAGTTCCC TGCTGGCCCA GTTCTTCTCT 
TGCGTCTCTT GTTGGGATCT TCGTGGCTCC CTGCTGTCCC TGCGGGAGGG TCAGGCACTA 
TCTGTGGCAG GGGGCCTGCC CTCTAATCTC TCAGAGGGCC TGCGCCTCGG CCCCATGAAT 
CTCCAGGACC CCTTTGACCT GAGTCACAAT GTGGCAGCCA ACGTGACCAG CCGGGTGGCC 
GGGAGGCTAC AGAACTGTTG CCGAGCGGCA GCCAATTACT GTCGAAGCCT CCAGTACCAG 
CGGCGGTCTT CCCGGGGTCG GGACTGGGGC CTGCTTCCCC TTCTGCAGCC CAGCTCCCCC 
AGCTCCATCC TCTCTGCAAC ACCTATCCCC TTACCCCCTG CTTCCTTCAC CCAGCTCACT 
GCTGTGCTGG CCCAGGTGTT AAGGGAAGCC TTAGGGTGCC ATATAGAACA GGGAACCAAG 
AGACTGCGGT CAGAGGGAGG TGGCCCTGGG GAGCCCCCCC AGGGAGGGAC AAGCAAGAGA 
GCAAAACTAG ATGGACAGAA AAAAAGCTGT GAGGAGGGGC CAGAGGAGCA GCAGGGATGT 
GCAGGGGAGC ATGGTGAAGA TGGCGTGGAA GAGATGGTCA TAGAGGTTGG AGAGTCGGTG 
CAGGACTGGG TGATGCGGAG CCCTGGGCAG CTGGGGGAGC TGCCCCTGAT GACCGGAAAG 
CATCTAGCCA CCAGAGAAGA GGGGCAGTCA GGCACCGCAG CGCTGGCCAA GCAGGGGCCC 
AGAGGACCTG AGGCAGCCTG CGAAGGGTCT CAGGCCGAGG CCGAGAAGCG GGTGTCGCTC 
ACAGTGAGCT GGCGCTGCGC CTTGTGGCAC CGTGTGTGGC AGGGGCGGAG GCGTGCCCGG 
AGACGCCTGC AGCAACAAAT CAAGGAAGGA GGTGGATCTG GTGCTGGCTC GGGAGCAGAG 
TGGCTGGCAA CTGAGGCTCA GGTCACCCGG GAGCTGCGGG GACTGAGCAG TACTGAACAG 
AGGCCAGAGG CTGAGCCACT CCTGACCTTC GTGGCATCCA CCTCCCAGGC TGACCAGAGT 
CTCACTGTGA CCCCACTCCA GGATTCTCAA GGCCTGTTCC CTGATCTCCA TCATTTCTTA 
CAGGTTTTCC TCCCTCAAGC ACTTCGAAAC CTGCTTAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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TUT1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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